LOCUS       DASWXU010000001         6531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_921595, whole genome shotgun sequence.
ACCESSION   DASWXU010000001 DASWXU010000000
VERSION     DASWXU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921595"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1754)
                     /locus_tag="VGE48_00005"
     CDS             complement(81..1754)
                     /locus_tag="VGE48_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY1041795.1"
                     /translation="MIFGRFRKRRRGLELNPDDIFLDASNLSGFDQSRLEGRLERPVT
                     ERTFATIGLVVGLLFIALIAQAANLQIMKGKEYAERSERNRLRPEVLFAERGAIVDRN
                     GTALVYNEQGEDGFVRRVYEYPGFSHLLGYVSYPKKDSSGNFYETEIKGLNGVEEAMD
                     TVLAGENGTLLVEEDAHGDLQSSGTVKPARKGETVTLSIDARAQRAFANAVSELADRI
                     PFMGGSAILMDVHTGEIHALVSYPEYDPNVLSSGGPSDVIASYQTNSRRVYLDRAVSG
                     LYTPGSILKPMVAAGALTDGIVSPSWGIVSTGSISIPNPYDPSKPSIFKDWKAHGWVD
                     ARRAIAVSSDVYYYAIGGGYGDVKGLGIERLKYWYDAFGLTSPTGIELSGEATGFVPT
                     PAWKEERFGDPWRLGDTYNTSIGQYAMQVTPLGAARAVAAVANGGMLVKPTLVKDAPL
                     QGQTIQISQSALQVAREGMRMGATEGTSIGLNALPFVKVAGKTGTAQLGVNNEFYNSW
                     SVGYFPYDKPKYAWVVVMERGPSTNAIGGIYVTYQALSELHRTAPEYFE"
     gene            complement(1751..2002)
                     /locus_tag="VGE48_00010"
     CDS             complement(1751..2002)
                     /locus_tag="VGE48_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041796.1"
                     /translation="MRVMSIILALIALFIFPWQVALFAVFGASLLTPLSGVALGIIAD
                     LVYYVPGAAFAPYFSLFGLIATGIGFLVQRFVKTRIIGE"
     gene            complement(2002..2790)
                     /gene="mreC"
                     /locus_tag="VGE48_00015"
     CDS             complement(2002..2790)
                     /gene="mreC"
                     /locus_tag="VGE48_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY1041797.1"
                     /translation="MKKRFSRRHNSIVGAYPWLGPVALGLFVLVLIFGMLRMFLPSAL
                     VVLGTPLWATGSALSAGVGNVSGFFANKPSLLEERDRLLSENAALYAKSATLEAKVAD
                     LTRLLGDRTEAGSGVLAGVLARPPVSPYDTLIVDQGERADVSVGMRAEGPGGMPLGTV
                     ESVSGGSARIILYSAPGKETESWIGEARIPVTLVGESAGAMSAVVAREAGIVPGDLVY
                     VAGSGARPVGTVVSVANDPSLPRSRVDIRPLANPFSITWVTLVP"
     gene            complement(2762..3823)
                     /locus_tag="VGE48_00020"
     CDS             complement(2762..3823)
                     /locus_tag="VGE48_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY1041798.1"
                     /translation="MAKKKRFSKNSLFSTDIGIDLGTGNTLVYVRGRGIVIAEPSVAT
                     VNEKTGQLVAVGKDAKAMLGKTPPHIRAVRPLSHGVISDFEVTEELLAYFINKAQGGR
                     SRLFGPRVVVGVPTGVTNVESRAVRDAAMTAGAREVFILEEPMAGAIGAKLPVMEAVG
                     TMVLDIGAGTTDIAVIALGGSVAGKSSQVAGDRFNDNIIDYVRSEFQLAIGEKTAEDT
                     KVAIGAVLPLAESLEKAVRGRDLATGLPREIILTDSHVREALAPSMEALMEAMKEVIE
                     RTPPELLSDVLERGVTVFGGGALLRGLPELLAKMLGVPVHVASDPLTAVVRGTGVVTE
                     DPRPWQDAFMHEEAILPKT"
     gene            complement(3826..5067)
                     /locus_tag="VGE48_00025"
     CDS             complement(3826..5067)
                     /locus_tag="VGE48_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEY1041799.1"
                     /translation="MRQSQLFTRTRREAPKDEEAKNAQLLIRAGFVHKELAGVYTYLP
                     LGLKTLNNINRIIREEMNAIGGQEIAMTALQDKQVWEKTDRWDDAKVDNWFKTEFKSG
                     GETGLGITHEEPITRMMSEHIASYRDLPAYAYQFQTKFRNELRAKSGIMRGKEFLMKD
                     LYSFSKDQEEHQAFFAKARDAYLKIFQRCGVGEQTYVTFASGGIFSAFSEEFQTLSDA
                     GEDTIYVDEEKRLAVNKEVMTDENLATLGLSREKLVEKKAIEAGNIFSLGTRFSEPLG
                     LTFMDEKGENKPVVMGSYGIGPTRLMGIIAEALSDEKGLVWPEEVAPFTYHLVSLAGS
                     YGNLQKIADELYADLMAQGKTVLYDDRDARAGEKFADADLIGLPYRIVVGKEALASGK
                     LEVVNRRTGEVSMLLKDELLK"
     gene            5270..5407
                     /locus_tag="VGE48_00030"
     CDS             5270..5407
                     /locus_tag="VGE48_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041800.1"
                     /translation="MHILGRYRVIRVRKEHQNPYCPYDPEELPEKQEMNPAQLDFLLA
                     A"
     gene            complement(5421..6521)
                     /locus_tag="VGE48_00035"
     CDS             complement(5421..6521)
                     /locus_tag="VGE48_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY1041801.1"
                     /translation="MTILIFALVLIALIVVHEFGHFVAAKWSGMRVDEFGLGYPPKAY
                     GKKIGETEYTLNWLPFGGFVKIYGEDPLPGQAPAPRSFTSRPRILQALVLVAGIAMNL
                     FFAYVLIAVTLGMGTTRALTEEEARMVPDARLAVASVLPESPAAEAGFKQGDLILSAS
                     ADDATFDGNDATAFTAFVAEEGVGSELSFVVQGQDGVERTLAATPEAGVVASAPERAA
                     LGVGLAVVGTHATPWWQAPIESAVLTWELTKQTAIALLQFFGSIFTLSADLAQVSGPV
                     GIAGAVGNASEDGIASLLTITALISINLALINLLPIPALDGGRLLFVIIESIIRRPIP
                     PTFANAVNGIGFLALILLMLVVTASDIFKIVG"
BASE COUNT         1355 a         2026 c         1823 g         1327 t
ORIGIN      
        1 tgctcggtca tgggtgtaat gaatgaaata tgaacgctgt tggttagggg tattgtacgg
       61 gaccccaacc gggcgtcaat ctattcaaaa tattcaggcg ccgtgcggtg caattctgag
      121 agcgcctggt acgtcacata aataccgccg atcgcgtttg tagacggacc gcgctccatc
      181 accaccaccc atgcgtactt cggtttgtcg tatgggaagt accccaccga ccacgaattg
      241 tagaactcgt tgttcacgcc gagctgcgcc gtaccggtct ttccggcaac cttcacgaac
      301 ggaagcgcgt tcagtccgat cgacgttcct tcagtagcgc ccatgcgcat gccttcgcgt
      361 gcaacctgga gcgctgactg gctgatctgt atcgtctgtc cctgaagcgg cgcgtccttc
      421 actagagttg gcttcacgag cattccgccg tttgcaaccg ccgcaaccgc gcgtgcagca
      481 ccaagcggcg taacctgcat cgcgtactgc ccgatcgacg tgttgtacgt gtcgccaagt
      541 cgccacgggt ccccaaagcg ttcctccttc catgcaggcg ttggtacgaa gccggtcgct
      601 tctcctgaga gctcgatacc agtagggctt gtgagaccga acgcatcgta ccagtatttg
      661 agccgttcga taccgagacc cttcacatcg ccgtacccgc cgccgatagc gtagtagtac
      721 acgtcggaag agactgcgat ggcacggcgt gcatctaccc agccgtgcgc cttccagtct
      781 ttgaagattg agggtttcga agggtcgtac gggttcggga tcgagatgga accagtcgaa
      841 acaatacccc acgagggcga gacgataccg tccgtaagcg caccggctgc caccataggc
      901 ttcaagatcg aaccaggggt gtagagacca gataccgctc gatcaaggta cacgcggcgg
      961 ctgttcgtct ggtatgacgc aatcacatca gacggacctc ccgaggaaag tacgttcgga
     1021 tcgtactcgg gatacgacac aagcgcatgg atctcccctg tgtgtacgtc catgagaatc
     1081 gccgagccgc ccatgaacgg gatgcggtcc gcaagctcgg atactgcgtt tgcaaatgcg
     1141 cgttgcgccc gtgcatcgat cgaaagcgta accgtctcac ctttgcgtgc aggcttcacg
     1201 gtacctgaag actgcagatc cccgtgcgca tcctcctcaa caaggagcgt gccgttttct
     1261 ccagccaaga ctgtatccat ggcttcctcc acaccattca gtcccttgat ctccgtctca
     1321 tagaaattgc ctgaggagtc cttcttcggg tacgacacat acccaaggag atgcgaaaat
     1381 ccagggtatt cgtacacgcg ccgcacgaac ccgtcctcac cctgctcgtt gtatacgagc
     1441 gccgtaccgt ttcggtcaac gatcgcgccg cgctcagcga acagtacctc aggacgtagg
     1501 cggttgcgct ccgagcgctc cgcgtattcc ttacccttca tgatctgcag gttcgctgcc
     1561 tgcgcgataa gcgcgataaa gaggagacct acgacgagtc cgatcgtcgc aaacgtgcgc
     1621 tcagtcacag gacgctccaa gcgcccttca agcctgctct gatcgaaccc tgacaggttc
     1681 gatgcgtcga ggaatatgtc gtcagggttg agctcgagcc cgcgcctgcg cttcctaaat
     1741 cgcccgaaga tcattcccct atgatacgcg tcttcacgaa acgctgcacg aggaatccga
     1801 tgccagtggc gatgaggccg aagagagaga aatagggcgc aaatgccgct cctgggacgt
     1861 agtacacgag atcagcgatg atgccgagcg cgacacctga gagcggcgtg agcagggatg
     1921 cgccgaacac cgcgaacaag gctacctgcc atgggaatat gaagagcgcg atcagcgcga
     1981 ggatgatcga catgacacgc attacggcac gagagtcacc catgtaattg aaaaggggtt
     2041 ggcgagcggc cgaatgtcga cgcgcgagcg cggcagcgac gggtcgttcg cgaccgagac
     2101 gaccgtcccg accggacggg cgccagaacc cgcgacatac acgaggtctc ctggaacgat
     2161 gcctgcttcg cgcgctacta ccgcactcat cgcacctgcg ctctcgccta ccaatgtaac
     2221 cgggatccgt gcctccccga tccaactctc ggtctcttta cccggagctg agtagaggat
     2281 gatgcgtgct gatccgcctg acacgctctc aaccgtgcca agcggcatac cgcccggtcc
     2341 ttctgcacgc ataccgaccg atacgtctgc acgctcgcct tgatctacga tcagcgtatc
     2401 gtacggggac accggaggac gggcgagtac tccggcgagc acaccgcttc ctgcttcagt
     2461 acggtctccg aggaggcgcg tgaggtccgc tacctttgcc tctaaagtcg cactcttcgc
     2521 gtagagagcc gcgttctcag acaagaggcg gtcacgctct tccagcaagg aaggcttgtt
     2581 cgcaaaaaat ccgcttacgt tccctactcc ggccgaaagc gccgaccccg tagcccagag
     2641 cggtgttcca agcactacaa gtgcgctcgg gagaaacatt cgaagcatcc caaatatcag
     2701 tacgagaacg aacaggccga gcgctaccgg tccgagccag ggatacgccc ctactatgga
     2761 gttatgtctt cgggagaatc gcttcttcat gcatgaatgc gtcctgccat ggacgcggat
     2821 cttccgttac tacgcccgtg ccgcgcacca ccgccgtaag cggatcgctc gcgacgtgta
     2881 ctggcacacc gagcatcttc gcaagaagct cggggaggcc acgcaacagc gcgccaccac
     2941 caaacactgt tacaccgcgc tcaagcacgt ccgagagaag ttcgggtggc gtcctctcaa
     3001 tgacctcctt catcgcttcc ataagcgcct ccatggacgg cgcgagcgcc tcgcgcacgt
     3061 gggaatccgt gagaatgatc tcgcgcggaa ggccggttgc gaggtcgcga ccgcgcaccg
     3121 ccttctcaag ggattctgcg agcgggagca ccgcgccaat agccaccttc gtgtcttcag
     3181 cggtcttctc gccgatcgcg agctggaact cgctcctcac atagtcaatt atgttgtcat
     3241 tgaaccggtc tcctgccacc tgagagctct tcccggcgac cgagcctcca agtgcgatga
     3301 ccgcgatatc ggtcgtgcct gcgccgatat caagtaccat ggtacccact gcctccatga
     3361 ccgggagctt cgcgccgata gcgccggcca taggctcttc gagtatgaat acttcgcgcg
     3421 cgccggccgt catcgccgcg tcgcgcaccg cccggctctc cacgttcgtg acgccagtag
     3481 gaacccccac caccacacgc gggccgaaca gccgtgagcg cccgccctga gccttgttta
     3541 tgaaatacgc gaggagctct tccgtaacct cgaaatcaga gatcacgccg tgcgagagag
     3601 gacgcaccgc gcggatatgc ggcggcgtct tgccgagcat cgcttttgca tcctttccca
     3661 ccgcaaccag ctgtcccgtc ttctcgttca cggtcgcgac cgacggctcc gcgatcacga
     3721 tgccgcgtcc gcgcacgtat acaagcgtgt tgccggtacc gaggtcgata ccgatgtcag
     3781 ttgaaaacag agagtttttc gagaatcgct tcttctttgc catacctact tcaggagctc
     3841 gtccttgaga agcatcgata cctctccagt cctgcgattc acgacttcga gcttgccgct
     3901 tgcgagcgcc tccttcccta ccacgatgcg gtacggcagg ccgatgaggt ccgcgtccgc
     3961 gaacttttct cccgcgcgtg cgtcgcgatc atcgtagagc acggtcttgc cttgtgccat
     4021 aaggtcagcg tagagctcgt cagcgatctt ctgtagattc ccgtagctcc ctgcaagcga
     4081 gacgaggtga tatgtgaacg gcgcaacctc ttccggccat acaaggcctt tctcatccga
     4141 gagcgcctcc gcgataatac ccatgagacg ggtcgggccg atgccgtagc tacccatgac
     4201 caccggcttg ttctctcctt tctcgtccat gaacgtgagg ccgagtggct cggagaagcg
     4261 ggtgccgagg ctgaagatgt ttccggcttc gatagccttc ttctcaacga gcttctcgcg
     4321 cgaaaggccg agcgtcgcga ggttctcgtc cgtcatgacc tccttgttaa cagcgagccg
     4381 tttctcttca tctacgtaaa tagtgtcctc gcctgcatcc gacagcgtct ggaactcctc
     4441 agagaacgcg ctgaaaatgc ccccggacgc gaacgtgacg tacgtctgct ccccgacgcc
     4501 gcagcgctgg aagatcttta ggtacgcgtc gcgcgctttc gcgaagaacg cctggtgctc
     4561 ctcctggtcc ttcgagaacg agtagaggtc cttcataagg aactccttgc cgcgcatgat
     4621 gccggacttc gcacgcagct cgtttcggaa cttcgtctgg aactggtacg cgtacgccgg
     4681 gagatcgcga tacgaggcaa tgtgctcgga catcatgcgc gtgatcggct cctcgtgcgt
     4741 gataccaaga ccagtctcgc ctcctgattt gaattcggtc ttgaaccagt tgtcgacctt
     4801 cgcgtcgtcc cagcggtcgg tcttctccca gacctgcttg tcctgaagcg ccgtcatggc
     4861 gatctcctgc ccgccgatcg cattcatctc ttctcggatg atgcggttga tgttgttgag
     4921 ggtcttgagg ccaagcggca gatacgtgta gacgccggcg agttccttgt gcacgaaacc
     4981 ggctcgaata agaagctgcg cgttctttgc ttcctcatcc ttaggggcct cgcgtctcgt
     5041 tcgcgtaaaa agctgggatt gccgcatagt tccatcagta tacggtttca attacctggg
     5101 gggaagccat acttgaaata attaataagt attgtacagt gatctcgtac acaggagttc
     5161 cccaatgccg atggttgcct acgacttgca tgtcgcgcga gtccggcatt gctgcgactt
     5221 gtgtgatcag ccgatctttc ccggagacca gtacgcaagg atcgtgtcct tgcacatact
     5281 cgggcgctat cgggtcatac gggtgcgcaa ggaacaccag aacccgtatt gcccgtacga
     5341 cccggaagaa cttccagaaa agcaggagat gaatcctgca cagctcgact tcctcttggc
     5401 cgcctagtgc ggcctttctc ttatccgact atcttgaaga tatcgctggc cgtaaccacc
     5461 aacataagga ggatgagcgc gagaaatccg atgccgttaa ccgcgttcgc aaatgtcggc
     5521 gggatcggac gacggatgat ggattcgatg ataacgaaga ggaggcgtcc gccatcgagc
     5581 gccgggatcg gcaacaggtt tatgagcgcg aggtttatag agatgagcgc agtgatagta
     5641 agaagcgatg cgattccgtc ctccgacgcg ttcccaaccg ctcctgcgat acccacggga
     5701 cccgagacct gcgcgaggtc cgcagagagc gtaaatatac tgccgaagaa ctggagaagc
     5761 gcgatcgcgg tctgcttcgt cagctcccac gtaaggactg cgctctcgat aggcgcctgc
     5821 caccacggcg ttgcgtgcgt gccgacaact gcaagtccga cgccgagtgc tgcacgctca
     5881 ggtgccgacg cgacaactcc cgcttcaggc gtcgctgcga gcgtgcgctc gacaccgtcc
     5941 tgaccttgca ccacgaatga gagttcgcta ccaacaccct cttcagctac gaacgcggta
     6001 aatgcggtcg catcgttccc gtcgaacgtc gcgtcgtctg ctgacgcaga gaggatgagg
     6061 tctccctgct tgaagcccgc ctctgctgcc ggagattccg gaagcacgct tgcgaccgca
     6121 agacgcgcgt ctggcaccat gcgcgcctcc tcttctgtga gtgcgcgggt agtacccatg
     6181 ccgagcgtga cagctataag gacgtatgcg aagaagagat tcatcgcgat gccagcaacg
     6241 agcacgagcg cctggaggat gcgtggtcgc gaagtgaagg aacgcggcgc aggagcctgt
     6301 cccggaagcg gatcctcgcc gtagatcttc acgaaacccc cgaacgggag ccagttaagg
     6361 gtgtattccg tctccccgat cttcttcccg tatgcttttg gcggatagcc gagtccgaac
     6421 tcgtccacac gcatgcctga ccacttcgcc gcaacgaagt gcccgaactc gtgcacgaca
     6481 atgagggcaa taaggacgag cgcgaagata aggatagtca tgtgctagat g
//
LOCUS       DASWXU010000002         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_210162, whole genome shotgun sequence.
ACCESSION   DASWXU010000002 DASWXU010000000
VERSION     DASWXU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210162"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VGE48_00040"
     CDS             complement(<1..616)
                     /locus_tag="VGE48_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY1041802.1"
                     /translation="MPKKAKQEEAPIEPRVNVVDQSITSEMRTSYLDYAMSVITSRAL
                     PDVRDGLKPVHRRILYAMRQTGLTPTAKFRKSATVVGEVLGSYHPHGDASVYDAMVKL
                     AQDFATRYLLVQGQGNFGSVDGDPPAAYRYTEAKMSRLADEMLRDLDKETVEWNPNFD
                     GSKQEPSVLPAAVPNLLLNGTLGIAVGMATNIPPHNLREVVAATT"
     gene            complement(622..999)
                     /locus_tag="VGE48_00045"
     CDS             complement(622..999)
                     /locus_tag="VGE48_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041803.1"
                     /translation="MATERIIEHLDRLREKPQHVRHQIAMATAFGVTSLVALGWVAAL
                     ATSGTLALKDAPAQADGADQALAASVQESSSAFSNLMGAAGAAFTATSTEAALRIIDT
                     RTSSTLDARSEPANATSKTVIPF"
     gene            complement(1003..1644)
                     /locus_tag="VGE48_00050"
     CDS             complement(1003..1644)
                     /locus_tag="VGE48_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1041804.1"
                     /translation="MVITHHGGQCFKVTLGDLTLVFDPIAKGASLPAVRFGADLALVT
                     RNHKDMNGTDEVSFGGKEPFAINGPGEYEHSGVTVQGFLTKSQYGLPKGQLDAVNTVY
                     AVSLEGMTLVHLGALSDPMLSHDAREAIDDIDVLFVPVGGDGVLSPAEAAKIAVTLEP
                     KIVIPMHFEGMGEAKALQSFLKESGKDCETVDKLTLKKKDAADKDGAIIVITA"
BASE COUNT          362 a          642 c          638 g          418 t
ORIGIN      
        1 tcgtggtcgc cgcgacgacc tcacgcaggt tatgcggcgg gatgttggtt gccataccca
       61 ccgcaatacc gagcgtgcca ttgaggagca ggttcggcac ggctgccggc agcaccgacg
      121 gttcctgctt cgagccgtcg aagttcggat tccactcgac ggtctcctta tccaggtcgc
      181 ggagcatctc gtcggcaagc cgagacatct tcgcctcggt atagcggtat gccgcaggcg
      241 ggtctccatc gacggagccg aagtttccct ggccctgcac caagaggtag cgggtcgcga
      301 aatcctgcgc gagcttcacc atagcgtcgt acacagacgc atcgccgtgc gggtgatagc
      361 taccgagcac ctcgcccacc accgtcgctg acttgcggaa cttcgccgtt ggcgtgagac
      421 cggtctggcg catcgcgtag aggatgcggc gatgcaccgg ctttaagccg tcgcgcacat
      481 ccgggagcgc gcggctcgtg atcacggaca tcgcataatc gagatacgac gtgcgcatct
      541 cgctcgtgat cgactgatcc accacgttca cgcgcggttc aatcggtgct tcttcctgtt
      601 tcgctttctt tggcatggac actagaacgg aatcacggtc ttcgacgtcg cgttcgctgg
      661 ctcgctgcgc gcatcaagcg tgcttgaagt gcgggtatcg ataatgcgga gcgccgcctc
      721 ggtgctcgtt gcggtaaacg cagctcccgc ggcgcccatg aggtttgaaa aggcagacga
      781 cgattcctgc accgacgccg cgagcgcctg gtctgcgcca tctgcctgcg ctggcgcatc
      841 cttaagcgca agcgtcccag aggtggcgag cgcggctacc cagccgagcg ccacgagcga
      901 ggtgacgccg aacgcggtcg ccatcgcgat ctggtgccgg acgtgctgcg gcttctcgcg
      961 cagacggtcc aggtgctcga tgatgcgttc ggtggccata ggctatgcgg ttatcacgat
     1021 gatagcaccg tctttgtccg cggcatcctt cttcttgagc gtgagcttgt ccacggtctc
     1081 gcagtccttc ccgctttcct tgaggaagct ctgaagcgcc ttcgcttcgc ccatgccctc
     1141 gaagtgcatc gggataacga tcttcggttc gagcgttacg gcaatcttcg ccgcttccgc
     1201 aggcgacagg acgccgtccc cgcccacggg cacgaacagc acgtcgatgt cgtcgatcgc
     1261 ctcgcgtgcg tcatgcgaga gcatggggtc tgagagcgcg ccgaggtgca cgagcgtcat
     1321 gccctcgagc gacacagcat ataccgtgtt caccgcatcg agctgcccct tcggaagccc
     1381 gtactggctc ttcgtaagga agccctggac cgtgacgcct gagtgctcat actcgcccgg
     1441 cccgttgatc gcgaacggct ccttgccgcc gaaggagact tcgtcggtac cgttcatgtc
     1501 tttgtggttg cgcgtgacaa gcgcgagatc cgcgccgaag cgtaccgcag gcagcgacgc
     1561 gcccttcgcg atcggatcga agaccagggt gaggtctccg agggttactt taaagcactg
     1621 cccgccgtgg tgggtgatga ccatgttctc ttcattatac cgcagaacgg ggtgctgtgc
     1681 acaggcagga gggcctgagg aagcagctgc ttgctagagt aaaggggcgc tacgcgcagg
     1741 gagctggcag caagacgagg gccgggtttt ctccgggtta ccgaccgcgt gcagccagct
     1801 ccttccttcc ctcacccgcc actttggcgg gttttgtttt atattgacaa ataaagtgta
     1861 tgatggtatc atcatatacg gaaagggttg cacacgcagc cctatgtgta ttgaaaagga
     1921 actgtgccat gtccgcgaga acctcgtgga ccacaccgat ttcgtcaccg cccacccggc
     1981 gcagtccctg caaggggttc gcgcgccgca gctgggcatc aaggaccgtc cggtctcctg
     2041 ctatcggctg gagggcggcg
//
LOCUS       DASWXU010000003         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_210257, whole genome shotgun sequence.
ACCESSION   DASWXU010000003 DASWXU010000000
VERSION     DASWXU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210257"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..988)
                     /locus_tag="VGE48_00055"
     CDS             complement(194..988)
                     /locus_tag="VGE48_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY1041805.1"
                     /translation="MRYFDAHCHVQFDPYDEDRVAVLNRMIEQEVGGMVVGVDLDSSV
                     KALRLVETLPNFYAAAGLHPNYVLDERFDVDSFRALLRHPKMVAVGECGLDNFRPEDV
                     AVAKVEQKKVFEQHVELAIEADKPMMIHSRPSKGTQDSYRDMIDILRSYKQEHGDRLR
                     GDIHFFVGGVEEAKGFMDLGFTLSFTAVLTFARDYDEVVRYVPLSSIITETDAPYIAP
                     LRIRGKRNEPTSVIDVVEAIAGIREEDQELVREAVLSNAKDLFKLS"
     gene            complement(988..2430)
                     /gene="metG"
                     /locus_tag="VGE48_00060"
     CDS             complement(988..2430)
                     /gene="metG"
                     /locus_tag="VGE48_00060"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY1041806.1"
                     /translation="MARYLTTTLPYVNADPHIGFALEIVQADALARSWRLQGEEVFFS
                     TGTDEHGQKIFQAAEKAGKEVQEYVDHYAKEFEKLKGALSITNDAFIRTTDLSHIRAA
                     QEMWRRCAASGDIYKKSYTGRYCVGCESFKVEKDLSEDGHCLIHTNLVAEEITEENYF
                     FRFSKYQDQLLSYLSKENVIQPDWRRQEAINFVSGGLEDFSISREKARLSWGIPVPDD
                     ESQVMYVWFDALTNYISTLGWPEEGGKFKAFWEDGNVIQVAGKDQVRFQSLMWQAMLM
                     SANVKNTDTIFYHGFINSGGQKMSKSLGNVISPYELVEKYGTEATRYMLLRHVHPSED
                     SDVTWERMDEWYTANLVNGLGNLVARVMKLAGDNLEGPVTLESSDTTIDEAFSHALDA
                     FRFNDAMDYVFAIIAECDAYMTAKEPFRKVKSEDRGIQEEGREDLRFLVRKLALIATH
                     LVPLMPQTADTVLKAVETNTKPENLFPRLS"
     gene            complement(2437..3495)
                     /locus_tag="VGE48_00065"
     CDS             complement(2437..3495)
                     /locus_tag="VGE48_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY1041807.1"
                     /translation="MQKNVSITITPGTIIMALLIGAAAYALWLLRDIALLVLTAIVIA
                     SAIEPGVTFFTRRRIHRVVAVTVMYLVVFGALFGLVYAFVPPVLEEAQGFLSSAPQYL
                     DTLNLPTSLTGVAETARTATAGQSQSIFDTIFAFQSAFADTSGGVVRVVSTFFGGIFS
                     LFLVIVLSFYFAVQSTGVEDFIRLLSPAKHEQYAVGLWLRAQRKIGQWMQGQLLLSLI
                     SGTITYLGLLILGVPYALLLAVIAAVMNLVPVFGSLIAGVFATVVAFGAGGVTLALIV
                     AGLFLIINQFEGNLLYPLVVNKVVGIPPLLVILALLVGGSLAGFLGVLLSIPLAAAFR
                     EFLSDIERNKRLRAAHEA"
     gene            complement(3988..>4464)
                     /locus_tag="VGE48_00070"
     CDS             complement(3988..>4464)
                     /locus_tag="VGE48_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY1041808.1"
                     /translation="SLTYLDEEAKEKIVPHVIEPSLGLERTMLAVLASAYDEDELGGE
                     KRVVLRLSPKVAPVKAMVSPLLKNKPELVAKAKEVRAALKKAHPAIAWDDNGNIGKRY
                     RRQDEIGTPFCVTVDFDTLGETPELLGTVTLRHRDTGEQERLTVEEVIERITSALS"
BASE COUNT         1014 a         1327 c         1201 g          922 t
ORIGIN      
        1 tggtacgtct tcttcacctc ttcctgcggc aattcaggat cgatatggaa gccaagctcg
       61 tagacgcggg tctcgttatg acctgcggcg agctcaagct cggtgctgcc tacaccgtgt
      121 tcgatgtcgt tattcatgac ccgctgactg taccacgcaa gacaaaacct agcaatgaag
      181 ccgatttttg ggcttatgag agtttgaaga ggtccttcgc gtttgaaagc actgcttcgc
      241 ggaccagctc ctgatcttct tcacgaattc ctgcaatagc ctcaaccaca tcaataaccg
      301 acgtcggctc attccgcttc ccgcggatgc gtagaggagc gatatagggc gcatcggtct
      361 ccgtaataat gctcgagagc ggcacgtacc gcaccacctc gtcatagtca cgcgcgaacg
      421 taaggaccgc cgtgaatgag agcgtaaacc caagatccat gaaacccttc gcctcctcta
      481 ccccacccac gaagaaatga atgtctcccc gaagacgatc tccgtgctcc tgcttgtacg
      541 agcggagaat gtcgatcatg tcgcgatacg aatcctgagt tcctttggat ggccgcgagt
      601 ggatcatcat aggcttatcc gcttcaatag cgagttcgac gtgctgctcg aatactttct
      661 tctgttctac tttcgcaacc gcaacgtcct ctgggcggaa gttatcaagt ccgcattccc
      721 ccaccgctac catcttgggg tggcgtagta aggcacggaa cgaatctaca tcgaagcgct
      781 cgtccagcac atagttcggg tgtagtcccg cggccgcata gaaattcggg agcgtctcca
      841 ccaaccgaag cgccttcacc gaagagtcca gatccactcc cacgaccatg ccacccactt
      901 cctgctcgat catgcggtta agcaccgcta cgcggtcctc gtcatatgga tcgaactgaa
      961 catgacagtg cgcgtcgaag tatcgcatta ggaaaggcgc gggaagagat tctcgggctt
     1021 cgtattggtc tctaccgcct taaggaccgt atccgcggtc tgtggcatga ggggcacgag
     1081 atgggtggct atgagcgcga gcttgcgcac aaggaaccga agatcctccc tgccctcttc
     1141 ctgtattcct ctgtcctcgc tcttcacctt tctaaaaggc tcctttgcgg tcatgtacgc
     1201 atcgcactcg gcgataatcg caaacacgta atccatagcg tcattaaagc ggaacgcatc
     1261 aagcgcatgt gagaacgctt cgtcgatggt cgtgtctgaa gactcgagcg taactggtcc
     1321 ctcaagattg tctcctgcaa gcttcatgac gcgtgcgaca agattcccaa gaccattcac
     1381 gagattcgcg gtataccact catccatgcg ctcccacgta acatcggagt cctcgctcgg
     1441 gtgcacgtgc cgcagaagca tatagcgcgt cgcctcagta ccgtactttt ccacgagttc
     1501 atatgggcta atcacgttcc caagagactt cgacatcttc tgtccgcccg aattaatgaa
     1561 gccatgatag aagatcgtgt ccgtattctt cacgttcgcg gacataagca tcgcctgcca
     1621 cataagggac tggaagcgca cctggtcctt cccggctacc tgaatcacat tgccatcttc
     1681 ccagaacgcc ttgaacttgc cgccctcctc tggccaaccc agggttgaaa tgtagttcgt
     1741 gagcgcatca aaccagacat acatgacctg gctctcgtcg tccggcacgg gaatgcccca
     1801 tgagaggcgc gccttctcgc gcgagatgct gaaatcctcg agtcctcctg atacgaagtt
     1861 gattgcctcc tgacgacgcc agtccggctg tatgacgttc tccttcgaga gatatgaaag
     1921 gagctggtcc tgatacttcg agaaacggaa gaagtagttc tcttccgtta tctcctccgc
     1981 tacgaggttc gtatggatga ggcagtgtcc gtcttcagaa agatcctttt caaccttgaa
     2041 cgactcgcag cccacgcagt atctgccggt gtagctcttc ttatagatgt cgccagacgc
     2101 ggcacagcgg cgccacatct cctgcgcggc acgaatatgg gagagatccg tagtgcggat
     2161 aaacgcgtca ttggttatgg aaagcgctcc cttaagcttc tcgaactcct tagcgtagtg
     2221 gtccacgtat tcctgtacct ccttccctgc tttctcagca gcctggaaga tcttctggcc
     2281 gtgctcgtcg gtaccagtcg agaagaacac ctcctcaccc tgaaggcgcc acgagcgcgc
     2341 aagcgcatca gcctgcacga tctcaagcgc aaacccgata tgcgggtccg cattcacgta
     2401 tggaagcgtt gtggttaggt agcgagccat gggcgattac gcctcgtgcg cagcccggag
     2461 gcgcttattg cgctcgatat cagagaggaa ctcacggaat gccgcagcaa gtggaattga
     2521 gagaagcacg ccgaggaacc cggcgagact gccgcccaca agaagcgcga ggataacgag
     2581 aagcggcggg ataccgacca ccttgttcac cacaagcgga tacagcaagt tgccttcgaa
     2641 ctggttgatg atgaggaaga gtcccgcgac gatgagcgcg agcgtaacgc cccctgcccc
     2701 aaacgcaacg acggtagcga acacgccagc gatgagagaa ccgaataccg gcacgaggtt
     2761 catcacggcc gcgataaccg caaggagaag tgcatacgga accccaagaa taaggagacc
     2821 gagatacgtg atggttcctg aaatgagaga caggagaagc tgtccttgca tccactgccc
     2881 tatcttgcgc tgggcgcgca gccagagacc caccgcgtac tgctcatgct ttgctggaga
     2941 aaggagacgg atgaagtctt cgacacccgt ggactgcacc gcgaagtaga acgaaagcac
     3001 gatcacgagg aacaaggaga agatgccgcc gaagaatgtg gacacgacgc gcaccacgcc
     3061 gcctgaggta tctgcgaacg cagactggaa cgcgaagatc gtatcgaaga tggactgcga
     3121 ctgtcctgca gtcgccgtgc gtgctgtctc tgcaacgccc gtgagcgatg tcggaagatt
     3181 aagcgtatcg aggtactgtg gcgcacttga gaggaagccc tgcgcttcct cgagtaccgg
     3241 aggcacgaac gcgtacacga gcccgaagag cgctccgaag accacgaggt acatgaccgt
     3301 aacggctacg acgcgatgga tgcgacgacg tgtaaagaat gtcacgcctg gctcgattgc
     3361 ggacgcgata acaatcgccg tgagtacgag aagtgcgata tctcgcagca accagagcgc
     3421 gtaggctgcc gctcctatga ggagcgccat gatgatcgtg cccggggtga tggttatgga
     3481 gacgttcttt tgcatgagga aaatggtacc acgaaaccga aaagcggcag ccgtattcat
     3541 ggctgccgct cgcctgcgcg ccagggcgtg cagaattggg accggactcc tggccggacg
     3601 aggtcgaagg acctggcctc gggaaaggag cacacaagca caatgaacgg atcgctccca
     3661 cgtacccgct cgaccgcctc gtcgagccct tcctttggaa ttaccatccc cgtgaggtcg
     3721 atgaagaacg ggatgtctcc ctcgctaccc cgctcatgta tccggaaagc cacgacctca
     3781 gtagccgggt tatgttccgg aattacagga gttggatccc aaaccatggg cttccctggt
     3841 ggcgtagtta tggtgagcgc ttcctgcgcc cgagcacctg taccccaaag gagcacagcc
     3901 gtgcccaaca gcagcatgca tagacgcatc tcgcttctcc tctggaacaa aagactagag
     3961 ggagcatgcg ctccctcttt cttctcgtca agaaagcgct gaagtgatgc gctctatgac
     4021 ttcctcaacc gtaaggcgct cctgttcgcc ggtatccctg tggcggagcg ttacagttcc
     4081 cagaagttcc ggtgtctccc cgagcgtatc gaaatcaacc gtaacgcaaa acggcgtgcc
     4141 gatttcgtcc tggcggcggt agcgcttccc gatgttgccg ttgtcgtccc acgcgattgc
     4201 cggatgcgcc ttcttgagtg ccgcacgcac ctccttcgct ttcgcgacca gttccggctt
     4261 gttcttgaga agcggcgaca ccatcgcctt cacaggcgcc accttcggag agagacggag
     4321 caccacgcgc ttctctcccc cgagctcgtc ctcgtcatac gcggacgcga gtaccgcgag
     4381 catcgtgcgc tcaaggccaa gcgatggctc gatcacgtgc ggcacgatct tctccttcgc
     4441 ctcctcatca agatacgtga gcga
//
LOCUS       DASWXU010000004        10187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_213637, whole genome shotgun sequence.
ACCESSION   DASWXU010000004 DASWXU010000000
VERSION     DASWXU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213637"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..828
                     /locus_tag="VGE48_00075"
     CDS             103..828
                     /locus_tag="VGE48_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041809.1"
                     /translation="MDAAVRTTKKYGYPKAYTGLKPLSEQIDTLASFAGRLGFEIDPA
                     PVHAVAARTESIALPEGAEGWGAILSPGLVGSERREREVMIELLRMQGPYDIDVRFEH
                     SEFFEEEYFRRDPLTQKMYETLSEQQPGDIHILPVQFGQRYAEVSDGEAIKNFGGPFG
                     EFGLGLLAGASLIATHPDRIGEYADLGVSLPGDQYHYRHSSKKFSRLCLVKQLGQRVT
                     LMHFKRDAEWMRDHGAASGFIWE"
     gene            complement(874..1572)
                     /locus_tag="VGE48_00080"
     CDS             complement(874..1572)
                     /locus_tag="VGE48_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009916 - alternative oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HEY1041810.1"
                     /translation="MNEEEFRAHEALVKELNDPVLRAEYARTYDTYKPGIVARVLGTI
                     LVGSGTLVYGKKPSYSKFKAIEVIARIPYQSWEVATYSLLTALYANEERAIELTKTSR
                     FSREAQDNETMHVVVLAHICKRLKCDGPIRHALIPLLFAFFYFWTIYVLYMLSRRSAL
                     ELNYLFENHAYHQYQEFLTREGERLRDTPVLSDFLAFYGRYPRNEYELFESIRNDELI
                     HRNRSIRELQAHAK"
     gene            complement(1592..3241)
                     /locus_tag="VGE48_00085"
     CDS             complement(1592..3241)
                     /locus_tag="VGE48_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1041811.1"
                     /translation="MTDTRLKDRITASIKGDVADDAKSRALFARDTSVFTRTPSVVVY
                     PKDADDVATLVRTVAGAKRDGIDASITARAAGTDMSGGPLTDSVVAVFTKYLNTVEVR
                     EGYAYTEPGAYYRDFEKATLQEGRQLMPSYPASRELCAMGGIVNNDSGGERSLEYGKT
                     RDYVLEMDVVLSDGSKATFKELSKEEVDKKVSEDTLEGRIYRDIRALITENRATIEAA
                     RPKVSKNSAGYALWDVMQEDGSMNLAKLLCGAQGTLALMTRAKLRLVKEQGYRSMLVV
                     FLDDITILPEIVRRVLPFNPESFESYDDHTFELAMKFLPQMLSQMGLAGAVKLGLSFL
                     PELGMILRGGVPKLVLMAEFSEDSREQALFQARKAELSLADLPVQTRIARDEQAAAKY
                     WKVRRESFALLRKNAKGLYAAPFIDDFVVPPSSYPEFMPKLNALLAEYDLLYTIAGHI
                     GNGNFHIIPLMNMKDERTRKIILELSPRVYDLVIQYGGTTTGEHNDGIIRTPYLTQLF
                     GSEMVALFARTKEIFDPNTIFNPGKKVGGTFEDIERSMIST"
     gene            complement(3262..4254)
                     /locus_tag="VGE48_00090"
     CDS             complement(3262..4254)
                     /locus_tag="VGE48_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HEY1041812.1"
                     /translation="MLKFFSMPIAVSVIILGLIWFYLGFGAFVTALLLMILEVTLSFD
                     NAVVNARVLADMDEKWRNRFLTWGILIAVFGTRLVLPVLIVAASVLVSPWAVAELALR
                     DPGAYGELLSGAKYGIYAFGSAFLLMVSLKYFFNSAKTTHWLRVVEHHLSKWGRIEAI
                     EIAVALSAIGVISLFVPAGTEATVLVAGIIGIVLFIVMQGITSAFSIEVDEGVVAKSG
                     LALFMYLEVLDAAFSLDSVVAAFALTTSLPIIVAGLGIGAWFVRSITIFLVRKRTLET
                     LTFLEHGAHWAILGLALCMFAGLVTHVPELVTGTIGLAFILAAYWSSIAHRKRA"
     gene            complement(4314..4553)
                     /locus_tag="VGE48_00095"
     CDS             complement(4314..4553)
                     /locus_tag="VGE48_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041813.1"
                     /translation="MYHITRLERPAHIVLAWGFLGLVCRYLAIVSAVFSVRCLSEPYP
                     FPVGRINSVEAGVAAIIVAMLCAWIYIWLRETDRC"
     gene            complement(4606..5010)
                     /locus_tag="VGE48_00100"
     CDS             complement(4606..5010)
                     /locus_tag="VGE48_00100"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY1041814.1"
                     /translation="MPNARALRRFMKYVLVGGSTFAFDLLLIWLMTEFLSVPYWLSTA
                     LGFAIAVSINYFVSRRFVFKGTARRIDHGYLFFILFAVGGSVFIAGAVALLVTLFAMH
                     YLAARILVAGIVGIGNYLFNLHFNFKVAGHHP"
     gene            5058..6002
                     /locus_tag="VGE48_00105"
     CDS             5058..6002
                     /locus_tag="VGE48_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1041815.1"
                     /translation="MTDTAPAILVTDLRKTYRKDGKTAGTEALKGVSLEIPKGSFFAL
                     LGPNGAGKSTLISILSGLVKKSEGEITVSGISASQHPEQTRAKIGLVPQELNLDIFGK
                     VLNIVVEQAGYYGIPRKEALVRAEEVLKSLSLWEKKDAKVQALSGGMKRRVMIARALM
                     HDPEILLLDEPTAGVDVELRRGMWDFLRELNQKGTTIVLTTHYLEEAELLADRVAIIN
                     GGEIIEEGTVKGLLTKLNFVTLLLDTTEPVKDAALVLLRELNIERVDENTLKLTLKPG
                     ETVNDAIRKISATGIQIANIRNTGSRLEEVFVNLIEKK"
     gene            6004..6777
                     /locus_tag="VGE48_00110"
     CDS             6004..6777
                     /locus_tag="VGE48_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009602132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1041816.1"
                     /translation="MTRSPALIGYYTLVRRDVFRLFRIWTQTLLPPAITMALYFAIFG
                     TFIGSQVGAISGYSYIQYIVPGLIMMSVITTTYLHVDMTFYFAKWQRTIEEQLVSPMP
                     GWVIIASFVSGGIIRAFLTGAIVIAVSLFFTHLSIFSLGVLFGAAFLTALLFSLLGLI
                     NGVFAKSFDDISIVPTFVLTPLTYLGGVFYSVEQLPELFRNLSLANPILYMVNAFRYG
                     FLGVSDISLAVCFGVMGGLVLVAFGVTVWLFKTGAALKQ"
     gene            complement(6839..7474)
                     /locus_tag="VGE48_00115"
     CDS             complement(6839..7474)
                     /locus_tag="VGE48_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041817.1"
                     /translation="MSSFGAAVAASTEQRPSVVRRETLWSGGIVAIRIPAGVTLGSYP
                     KDGKDIPFLQFSRCVRGGCVNLFVHTDDMSLRGPIVAGVSVMKKTLSDGREYLYIDLV
                     PVENDVAVTHFLDIVSRGEREPSPDDPVFETLGHIDGLVILTPPKAPKPPKPRKPKST
                     GDSQLDRLLNQGWEIDTETPTVVRLFKFVGETMKTMQHHKPKQKPLSKRKR"
     gene            complement(7843..8976)
                     /locus_tag="VGE48_00120"
     CDS             complement(7843..8976)
                     /locus_tag="VGE48_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1041818.1"
                     /translation="MQTHPSSKPSAAWATDLIDRWRGNLFLKTATYVVGLQLALALLT
                     IVLFVWGVGFVQDRTISAIGERIIQGIFNDTPASNESIAEAVLAVSWETYFVLASVLI
                     LLNVIFGYLIARFALWPTRNSLQFQKRFIGNIAHEIRTPLAIIKTNTEVALFDPALPE
                     NVKETFTDTLAELDRISETINNLLSFDTLTRPRRMTMEPTDVGDIAETALARHQALAT
                     SRNVTLESSIEGSRMVHGNAVALEQVVTNLVKNAINYTPKDKRGTVSVCVGPGETSGT
                     VKVTVRDTGIGIEQKDLFHIFEPFYRADTSRARGVGTGTSGLGLAIVNEIVRLHKGAI
                     SIRSTPGVGTEISVTLPAAKNGDLPDPLSDGTQGPNEVSFNFS"
     gene            complement(8918..9592)
                     /locus_tag="VGE48_00125"
     CDS             complement(8918..9592)
                     /locus_tag="VGE48_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1041819.1"
                     /translation="MRILIVEDEAKLAEGLKRGLESKGFAVDWIAESEKAKTRILLYR
                     DEYDLILLDLMLPGIDGATITRDARAEGVKTPIIILTARDETEYKVELLNSGADDYLA
                     KPFSFEELVARINAVLRRPEQSLPTKMTVRDIELDSSARTVTKAGKPVSLTLKEYSLL
                     EYFMRHPDEALTRESILDHVWSFDFPGFSNVLDVHMKNLRKKLDTYADAPLFETIRGV
                     GYRLNR"
BASE COUNT         2197 a         2855 c         3001 g         2134 t
ORIGIN      
        1 agaggggata aggaagaggt ctgtctagta gattttgaca cataccaatt tgtgttataa
       61 tatacataca gacattttgt cctatacata cggagctttc ccatggacgc agcagtgcgc
      121 accacgaaga aatacggcta ccccaaggca tacacgggcc tgaagcccct ttcggagcag
      181 atcgacaccc tcgcctcctt cgctggccgt ctcggcttcg agatcgatcc ggcgccggta
      241 cacgcggtcg ctgcacgcac cgagtcgatc gcgcttcctg aaggcgcgga aggctggggc
      301 gcgatcctca gtccgggcct ggtcggctcg gagcgtcgcg aacgcgaagt catgatcgag
      361 ctcctgcgaa tgcagggtcc ctacgatatc gacgtccgtt tcgaacacag tgagttcttc
      421 gaggaagagt atttccggag ggatccgctt acgcagaaga tgtacgagac cctgtccgag
      481 cagcagcccg gcgacattca catcctgccg gtccagttcg gacagcgtta cgccgaagtc
      541 tctgatgggg aagccatcaa gaacttcggc ggaccttttg gtgagttcgg cctcggcctg
      601 ctcgccggcg catcgctgat cgcgacccat cctgaccgca tcggcgagta cgccgacctt
      661 ggtgtgagcc tgcctggcga ccagtaccat taccgccaca gctcgaagaa gttcagcagg
      721 ctctgcctgg tcaagcagct gggccagagg gtgacgctca tgcacttcaa gcgtgacgcg
      781 gaatggatgc gtgaccatgg tgcagccagc ggcttcatct gggagtgatt ccccgaagcg
      841 acaaggccgc ctctccggag gcggcctttt ccattacttc gcgtgcgcct gaagctcgcg
      901 tatcgaacgg ttccggtgaa tgagctcgtc gttacgaatg gactcgaaga gctcgtactc
      961 gttgcgcgga tacctgccgt agaacgcgag gaagtcggat aagaccggcg tatcgcgcag
     1021 acgctcaccc tcacgcgtca gaaactcttg gtactggtgg tacgcatggt tctcgaagag
     1081 atagttgagc tcgagcgcag agcgtcgcga gagcatgtag aggacataga tcgtccagaa
     1141 gtagaagaac gcaaacagga gcggaataag cgcgtgacgg attgggccat cgcactttaa
     1201 gcgcttacaa atatgcgcga gcaccacaac gtgcatggtc tcgttgtcct gtgcttcacg
     1261 ggagaagcgg gaggtcttcg taagttcgat agctcgctcc tcgttcgcgt agagcgcggt
     1321 caggagggaa tacgtcgcca cttcccagga ctggtatggg atgcgtgcga taacctcaat
     1381 agctttgaac tttgagtacg atggcttctt gccgtacacg agcgtacctg agcctacgag
     1441 gatagtgccg aggactcgcg caacaatacc gggtttgtac gtgtcgtacg tacgcgcgta
     1501 ctcggcacgc agtactgggt cattcaattc cttcaccaga gcttcgtgtg ctcggaactc
     1561 ctcttcgttc atatgcgtaa tgtatcacgc gttatgtgct gatcatggag cgttctatgt
     1621 cctcgaatgt tccaccgacc ttctttccgg gattgaagat agtgttcgga tcgaatatct
     1681 ccttcgtccg cgcaaagagc gctaccatct cgctcccaaa aagctgcgtt aagtacggcg
     1741 tgcggatgat gccgtcgtta tgttcgcctg tggtggtgcc gccgtactgt atgacgaggt
     1801 cgtacacacg agggctaagc tcgaggatga tctttctcgt cctctcgtcc ttcatgttca
     1861 taagcgggat gatgtggaag ttgccgttcc caatgtgtcc ggcgatggtg taaaggagat
     1921 cgtactcagc aagcagcgcg ttcaatttgg gcatgaactc aggataggag gacggaggta
     1981 ctacgaagtc gtcgatgaac ggtgcagcgt aaaggccctt cgcgttcttt cgaagaagtg
     2041 cgaacgattc gcggcgtacc ttccagtact ttgcggccgc ctgctcatcg cgtgcgatgc
     2101 gcgtctggac tgggaggtct gctagcgaga gctctgcctt cctcgcctgg aacaaggcct
     2161 gctcgcgtga gtcctctgag aactcggcca tgaggacgag ctttggtacg ccgccacgga
     2221 gtatcatgcc gagctcaggt aggaaggaaa gtccgagctt cacggctccc gcgagtccca
     2281 tctgcgagag catctgcggc aaaaacttca tcgcgagctc gaaagtatgg tcgtcatagg
     2341 actcgaacga ttcgggattg aaggggagta cacgccttac gatctccgga aggatcgtga
     2401 tgtcgtctag gaataccacg agcatgctcc gatatccttg ctccttcacg agacgcagct
     2461 tcgctcgcgt cataagggcg agcgtgccct gtgcgccgca cagcagcttc gcaaggttca
     2521 tgctcccgtc ctcctgcatc acgtcccaaa gcgcatagcc ggcggagttc ttcgagacct
     2581 ttggacgcgc cgcttctatg gttgcacgat tctccgtgat gagcgcgcgg atgtcgcgat
     2641 agatacggcc ttcgagcgta tcctctgaga ctttcttatc tacttcctct ttcgagagtt
     2701 ccttgaatgt cgctttcgat ccgtcagaga gcaccacgtc catctcaagc acgtagtctc
     2761 gcgtcttccc atactcaagg gagcgctcgc cgccagagtc gttgttcacg atgccgccca
     2821 tggcacagag ctcccgtgaa gcagggtatg acggcatgag ctggcggcct tcttggagtg
     2881 ttgctttctc gaagtcgcgg taatacgcac cgggctccgt gtatgcgtag ccttcgcgta
     2941 cttccaccgt attcaggtac ttcgtgaaca ctgctacgac cgaatccgta agcggaccac
     3001 ccgacatatc ggtgcctgca gcgcgcgccg taatcgacgc atcgatgccg tctcgtttgg
     3061 cgcctgctac ggtacgcacg agtgtcgcga cgtcatccgc atctttcgga tacacgacta
     3121 cagacggtgt tcgcgtaaag acgcttgtgt cgcgcgcaaa gagcgcacga ctcttcgcgt
     3181 cgtctgcgac gtctcctttg attgatgccg taatgcgatc cttgagtctc gtgtcagtca
     3241 ctagactcga gtatagcgcg actacgcgcg cttgcggtgc gcgatcgagg accagtacgc
     3301 ggcgaggatg aacgcgagac cgatagtacc ggtcacaagc tcaggcacgt gtgtcacgag
     3361 acctgcgaac atacagagcg ccaagccaag gatcgcccag tgcgcgccgt gttcaaggaa
     3421 tgtgagcgtc tcgagggtgc gcttgcgcac gaggaagatg gtgatggagc gcacgaacca
     3481 cgcgccgatg ccgagacccg cgacgatgat agggagcgag gtcgtgagcg cgaacgcggc
     3541 cactacgcta tcgagagaga acgccgcgtc gagcacctcg agatacatga agagcgcaag
     3601 tccactctta gctaccacac cctcatccac ctcgatggag aacgcgctcg tgatgccctg
     3661 catcacgatg aagagcacga tgccgatgat gcccgcgaca agcactgtcg cttccgtgcc
     3721 tgcgggaacg aacagggaaa tcacgccgat tgcagagagc gcgaccgcaa tctcgattgc
     3781 ctcaatgcgg ccccacttgg agagatggtg ctccacgacg cgcagccagt gcgtggtctt
     3841 tgcactattg aagaagtact tcagagaaac catgaggagg aacgcgctgc cgaaggcgta
     3901 gatgccgtac tttgctccgg agaggagttc tccgtacgca cccggatcgc gaagcgcgag
     3961 ctccgctacc gcccacggac tcaccaacac gctcgcggcc acaatgagga ccggaagcac
     4021 gaggcgggtg ccgaacaccg cgatgagtat gccccaagtg aggaagcggt tgcgccactt
     4081 ctcgtccatg tccgcgagca cgcgcgcatt caccaccgcg ttatcgaacg agagcgtgac
     4141 ttcgaggatc ataaggagaa gcgcggtcac gaaggcgccg aacccgagat agaaccagat
     4201 gagccctagg atgatcacgg acaccgcgat cggcatggaa aagaatttca gcatatcgga
     4261 agattctagc agatacagat agggcccggc gtagtgccgg gccctctttc gcgctaacag
     4321 cgatcagtct ctcgaagcca tatgtagatc cacgcgcaga gcatcgccac gatgatagcc
     4381 gcaacgccag cttcaaccga gtttatcctt ccgaccggga acggatatgg ctcgctgaga
     4441 cagcgcacgc tgaacaccgc agatacaatc gcgaggtagc gacacacgag tccgaggaat
     4501 ccccatgcaa gcacgatgtg agcgggccgc tcaagccggg tgatgtgata catccgttcc
     4561 tcccatcctg ccggcactct acagtacgag gggacttatg caaccctacg gatgatgccc
     4621 agcaaccttg aaattgaagt ggagattgaa gagatagttt ccgatgccga cgataccggc
     4681 aacgagtatg cgcgccgcga ggtagtgcat cgcaaagagc gtaacgagga gcgccacggc
     4741 gccggcgatg aacacggatc ctcccacggc gaacaggatg aagaacaggt agccgtgatc
     4801 aatcctccgg gccgtgccct tgaacacgaa ccggcgtgag acgaagtagt ttatggagac
     4861 cgcgatagcg aacccgagcg cggtcgagag ccagtagggg acggacagga actccgtcat
     4921 gagccagatg aggaggaggt cgaacgcgaa ggtcgagccg cccacaagga cgtacttcat
     4981 gaagcggcgg agcgcgcgag cgttcggcat gtccccatga tagcccggaa aagagtcggt
     5041 ggtatactgc ccgagctatg actgataccg cgcctgccat cctcgttacc gacctcagga
     5101 agacctatag gaaagacggg aagacggcgg ggacggaagc tctaaagggc gtgtcgctcg
     5161 agattccaaa gggcagcttc ttcgccctcc ttggcccgaa cggcgctggc aagtctacct
     5221 tgatcagcat cctctcaggt ctcgtgaaga agtcagaagg ggagataacc gtctcaggca
     5281 tctcggcgag ccagcaccct gagcagacca gggcgaagat cggtctcgtg ccgcaggagc
     5341 tcaatctcga tatcttcggg aaggtactaa acattgttgt tgagcaggcc ggatactacg
     5401 gcatcccgcg caaggaagca ctcgtgaggg cagaggaagt cttgaagagt ctcagtctct
     5461 gggagaagaa ggacgcgaag gtgcaggcgc tttcaggggg catgaagcgt cgcgtgatga
     5521 ttgcgcgtgc tcttatgcac gaccccgaga tcctgcttct ggacgagcca acggccggcg
     5581 tcgatgtgga gctgcgccgc ggcatgtggg atttccttcg tgaactcaat caaaagggca
     5641 cgactatcgt gctcacgact cattaccttg aggaagcgga actccttgct gatcgcgtag
     5701 ccataatcaa cggcggtgag atcatcgagg aaggaacggt gaaaggtctc ctcacgaagc
     5761 tcaacttcgt gacgctcctt cttgatacga ccgagccggt gaaggacgcg gcgctcgttc
     5821 tgttgcgcga gctcaacatc gagcgcgtcg atgagaacac gctcaagctg accctcaagc
     5881 cgggcgagac cgtgaacgac gcgatcagga agataagcgc gaccggcatc cagatcgcga
     5941 acatccgcaa tacgggtagc cgccttgagg aagtgttcgt gaacctaatc gagaagaagt
     6001 aacatgaccc gctcacccgc actcatcggc tattacaccc tggttcgcag ggacgtgttc
     6061 cgtctcttta ggatctggac gcagacactg cttccgccag ctatcacgat ggcgctctac
     6121 ttcgccatct tcggtacgtt catcggaagc caggtgggtg cgatttcagg gtattcctac
     6181 atccagtaca tagtgccagg ccttatcatg atgtcggtca tcaccacaac ttatttgcac
     6241 gtggacatga ctttctattt cgcgaagtgg cagcgcacca tcgaggaaca gctcgtatct
     6301 ccgatgccgg gctgggtaat aattgcgagc ttcgtctccg gcggtatcat ccgcgcgttc
     6361 cttacgggcg ctatcgtgat cgcggtctcg ctcttcttca cgcacctctc gatcttctcg
     6421 ctcggtgtcc ttttcggagc agccttcctt acggccctcc tgttctcgct cctaggtctc
     6481 atcaatggcg tattcgcgaa gtccttcgac gatatctcta tcgttccgac ctttgtgctc
     6541 actccgctca cgtacctcgg cggcgtcttt tactcggtgg agcaactccc ggagctcttc
     6601 cggaacctct ccctcgctaa ccctatcctc tacatggtta atgcattccg gtacgggttc
     6661 ctcggcgtgt ccgacatttc gcttgcggtg tgcttcgggg tgatgggcgg actcgtcctc
     6721 gtcgcgtttg gagtcacggt ctggctcttc aagacaggag cggcgctcaa acaataagga
     6781 atacataaag aaagggcccg tccggatcgg acagggccct tgtggcagtt tttacgcttc
     6841 agcgtttgcg cttggacagc ggcttctgct tcggcttgtg gtgctgcatg gtcttcatcg
     6901 tttcaccgac gaatttgaac aggcgtacca cggtcggcgt ctcggtgtcg atttcccacc
     6961 cttgattgag caggcggtcg agctgcgagt cgccggtgga cttcggcttc cgcggtttcg
     7021 ggggcttcgg tgccttgggc ggagtcagga tgacgagacc gtcgatgtga cccagcgtct
     7081 cgaacaccgg gtcgtccggg gacggctcgc gctcgccgcg gctcacgatg tcgaggaagt
     7141 gcgtcaccgc gacgtcgttt tcgacgggga cgaggtcgat gtacaggtac tcgcgtccgt
     7201 cactcagcgt cttcttcatc acgctcacgc ctgcgacgat gggcccgcga agactcatgt
     7261 cgtcggtgtg cacgaagagg ttgacgcatc cacctcgcac gcagcgcgag aattgcagaa
     7321 agggaatgtc tttcccgtcc ttcgggtagc tcccgagggt tacgccggcc ggaatcctga
     7381 tggcgacgat gccgccggac cacagggttt cgcgcctcac cacagacggg cgttgttcag
     7441 tcgaggccgc aacagcggcg ccaaaactcg acatggcagt tctccagaat ccaaaagggc
     7501 aaaagtaccc ggtatatata ctacattata tatagaataa gtcaagaaac atgcaggcct
     7561 agccataaag agccggggcc ccgctaacga tttacgcagc gaggccccgg catcaatcct
     7621 tatgtttccg tatcatacag gcgcttcttg gcggttagcg tctggtaagg attcttcccg
     7681 tcgcatgccg tgcatcgtct ccgtggggat gcgcggcgta aataaggaga aggaaagaac
     7741 acccgaggcg gtgataggaa cacgcgtacg cgttccgtat catcctcgga ttagtactgt
     7801 acgcaaagcg aaatgaaccc gttatgaacg ggttcatgaa gctcaagaaa aattaaagga
     7861 aacctcgttc ggcccttggg taccgtcaga gaggggatca ggaagatcgc cgttcttagc
     7921 agcaggaagc gtgacgctta tctccgtacc aacgccaggc gtactgcgaa tggagattgc
     7981 acctttatgc aagcgaacga tctcgttcac aatagcgagg ccgaggcctg aagtgcccgt
     8041 tcccaccccg cgcgcgcgcg aggtgtccgc gcgatagaac ggctcgaaga tatggaagag
     8101 atccttctgc tcgatgccta tgccagtatc gcgtactgtc accttcacag tgcctgaggt
     8161 ctctcctggt ccgacgcata ccgacacggt cccgcgtttg tctttcgggg tgtagttgat
     8221 ggcattcttc acgaggttcg tgactacctg ctcgagtgct accgcgttgc cgtgcaccat
     8281 gcgcgaaccc tctatcgagg attcgagcgt tacgttacgg gaggtagcaa gcgcctgatg
     8341 gcgcgcgagc gcggtctccg cgatatcgcc cacgtcggta ggctccatag tcatgcggcg
     8401 tggacgcgtg agggtatcga aggacaggag gttgtttatg gtctctgaga tacggtcgag
     8461 ttccgcaagc gtatccgtga atgtttcttt cacgttctcg gggagcgcag gatcaaagag
     8521 cgccacttcc gtattcgtct tgatgatggc gagtggggtg cggatctcgt gcgcgatgtt
     8581 gccaataaag cgcttctgga actgcaggga atttcgggta ggccagagtg caaagcgggc
     8641 gatgaggtac ccgaagatga cgttgaggag tatgaggacc gaggcgagca cgaagtacgt
     8701 ctcccaggac accgcgagca cggcctcggc gatactctcg ttgcttgccg gggtgtcgtt
     8761 gaatatgccc tggattatcc gctcgccgat cgcggagatg gtccgatcct gcacgaatcc
     8821 tacgccccat acgaagagga cgatcgtaag gagcgcgagc gccagctgaa gacccacgac
     8881 atacgtcgcg gtcttcagaa acaggttgcc gcgccaccta tcgattaagt cggtagccca
     8941 cgccgcggat ggtttcgaag aggggtgcgt ctgcataggt atcgagcttc ttcctgagat
     9001 tcttcatatg tacatcaagc acgttcgaga agccagggaa gtcaaagctc cacacgtgat
     9061 cgaggatact ttcgcgcgtg agtgcctcgt ccggatggcg catgaagtac tcaagcagag
     9121 agtactcctt gagagtgagt gataccggct tcccagcctt cgttacggtg cgtgccgacg
     9181 agtcgagctc gatgtcgcga accgtcatct tcgtcgggag gctctgctcc gggcggcgaa
     9241 gcacggcatt gatgcgcgca acaagctcct cgaaggaaaa gggctttgca aggtagtcgt
     9301 cagcgcccga gttgagaagc tctaccttgt actcggtctc gtcacgggcc gtgaggataa
     9361 tgatcggcgt cttcacgccc tcagcgcgcg cgtcgcgcgt gatggtcgcg ccatcgatgc
     9421 ctgggagcat aagatcgagc aggatgagat cgtactcatc cctatagagg aggatgcggg
     9481 tcttggcttt ctcactctcg gcgatccagt ccacggcgaa tcccttggat tcgagaccgc
     9541 gcttgaggcc ttcggcgagc tttgcttcgt cttcaacgat gagtatgcgc atagcagggg
     9601 agttacggct aagaattagg tgtacgaaga ctgtatctag taaagcatga agcgttcatg
     9661 aaatggcatt gaaatgaaga aacccgccat aactgcgggt cattcggtgc gcgacgcggc
     9721 gcgagggaat ggtcttgaag gggattcaag agaccgttct ctcgcgcctg cgatttacgc
     9781 tttcagcggt tctgcatgct tcctcctacg gaacgttgaa aggacaccac cggtgggcgt
     9841 cgcccggcaa aagccttgcg gcgagtcatt cggccctcgg tacggggaat gtagcacgta
     9901 cttgtctgat gtctacgggc actcgattct gttcggggaa ctgttttata ccgaatcagg
     9961 tatcgccatt tatgctcccc aaacgcccag tatgtatgcc atatttcaaa cttgtgtctt
    10021 gcgcgaattc tcatatgttt cgtgctccac tgtccacacg gaagattcga tgaagagcat
    10081 ttagaataag ggaacaaccc agccgaatgc agtcgagagg gcgggtggac atgccaaggc
    10141 atcccacaca cgcgctcgaa gtggtccgaa gcaccctgcc agcgtcc
//
LOCUS       DASWXU010000005         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_671619, whole genome shotgun sequence.
ACCESSION   DASWXU010000005 DASWXU010000000
VERSION     DASWXU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671619"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VGE48_00130"
     CDS             complement(<1..1497)
                     /locus_tag="VGE48_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY1041820.1"
                     /translation="MQHLEGLNDAQREAVLATDGPISVLAGAGSGKTRVLTTRIYHLI
                     KNGTPAERILAVTFTNKAAREMKERLAHRLGDTPLPMVATFHGLGREILQRYGSRIGV
                     PRYFTIFDRDDSKDAVKQVLKEMDIDPKELSPGFVLSRISKEKGEGRTAEEFRESDGR
                     RSFQMRVVSEVWPRYEAIVRKEKALDFDDLICLPVHLLRDHEDIRREAEALYSYIHVD
                     EFQDTNGLQGRLAGYLAAKHKNLFVVGDIDQTIYTWRGATIENLLKFEETYPNAKTIV
                     LAHNYRSTKTIVAAGNQIIEKNENRKEKRAVTDNPDGERIKLHLARSAESEARYIARE
                     ARKALREGVPAEEIAVLFRTNFQSRALEEAFLGEGVPYKVLGTRFLDRREVRDVLAWV
                     RLALDPAREADRIRAAQAPSRGIGKVTLGKLAAGDREALRPAERAKVAAFEEVVASLH
                     EAALTLTPSEFVRLVIEKSGLETSLQKGGEEDQERLENAKELATLASRF"
     gene            1782..2801
                     /locus_tag="VGE48_00135"
     CDS             1782..2801
                     /locus_tag="VGE48_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1041821.1"
                     /translation="MLLPNAAGAFWPFSVTNASDGGSSVPMVHDSSLDLLEAATNPDP
                     NPAKGRGGVSMTGGTALIAYSGPEGTIADIDRASNNGKISTYVVREGDSLSGIADMFD
                     VSGNTILWANNIGKNDLRPGMSLIILPVSGVRYTVASGDTLASIAKKYSSDASEIASY
                     NGISSVDELVKGTTVIIPGGELASAPAAKAKTVAKSSSSAAKIKTGGSIGSVKSTGNT
                     SDSSFSGSFTNPAPAGRLSQGIHGWNGVDIAAPSGSPIYAAAGGTVIVSRVGGWNGGY
                     GNYVVIDHGNGVQTLYAHMSTDTVSVGETVSRGQSIGTVGNTGQSTGYHLHFEVRGAK
                     NPFGS"
     gene            complement(2860..3525)
                     /locus_tag="VGE48_00140"
     CDS             complement(2860..3525)
                     /locus_tag="VGE48_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEY1041822.1"
                     /translation="MGTLLVKGALSYGWNTFRARPWLFVQAGILLLLINLGVSLVQSV
                     FEAGAELGDGAVVAIVGLASAAFGMAVSFFVSMGETAFFLKAHDDVQKAALKDLWHPK
                     PFWNFVGASILAGIAIILGFIALIIPGIILGILFMFVGYLVIDQDLKPIDALKRSWEL
                     TKGNRWKLFFLSLALIGINILGLLALVVGLLVSVPVSFLAVTHAYRTLSGKAPETVED
                     TVE"
     gene            complement(3550..3651)
                     /locus_tag="VGE48_00145"
                     /pseudo
     CDS             complement(3550..3651)
                     /locus_tag="VGE48_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002145149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
     gene            complement(3925..>4585)
                     /locus_tag="VGE48_00150"
     CDS             complement(3925..>4585)
                     /locus_tag="VGE48_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041823.1"
                     /translation="TVPSIIVADEYEELSGTGGELMRALATSAGGAVVPGNVIVTYIL
                     EDMTEEGGGIVKTPAGGAAFLRALQVPAPDILLRNIARESAVGVVNAGSESSVFFALR
                     VDSYERTYAGMLTWEPLMLRDLSVLYPLYPIPAAPEPVASTTATSTAATSTPIMLDPV
                     PASALSRFSDAVVANHDVRILRDTSGRSLLLYGYADKRTLIIARDEAAFEALLPRLKA
                     E"
BASE COUNT          997 a         1323 c         1251 g         1014 t
ORIGIN      
        1 gaagcggctt gcgagtgtcg caagctcctt cgcgttctca aggcgttcct gatcttcctc
       61 cccgcccttt tggagcgatg tctcaagtcc cgacttctcg ataacaagac gtacgaactc
      121 agacggagtg agtgtgagtg ccgcttcgtg gagcgacgct acaacttcct caaacgcagc
      181 aaccttagcg cgctccgcag gtcgaagcgc ttccctgtcc cctgccgcaa gctttcctag
      241 ggttaccttc ccaatgccgc gacttggcgc ttgggcggca cggatacgat ctgcttcacg
      301 tgcaggatca agagcaagac gcacccatgc aagtacatcg cgcacttccc tgcgatccaa
      361 aaagcgcgta cctaagacct tgtacggcac tccttccccg agaaacgcct cttcgagtgc
      421 tcgagactgg aagttcgtgc gaaacagcac agcaatttct tctgctggaa cgccctcacg
      481 aagtgccttg cgtgcttcac gtgcgatgta gcgcgcttca ctctcggcgc tgcgcgcaag
      541 atgcagcttg atgcgttccc catcagggtt gtctgttacc gcgcgcttct ccttacggtt
      601 ctcattcttc tcaatgatct ggttgcccgc agcaacgatt gtcttcgtag atcggtagtt
      661 gtgtgcgagc acgatagtct ttgcgttcgg ataggtctcc tcgaacttaa gcaagttctc
      721 gatggtggcg ccacgccagg tgtagatggt ctgatcgata tcccccacca caaacaggtt
      781 cttatgcttg gctgcgaggt atccggcgag ccttccctgt agcccattcg tgtcctgaaa
      841 ctcatcgaca tgaatgtatg aatagagtgc ttccgcttcc cgacggatgt cttcgtgatc
      901 acgaagaaga tgcaccggaa ggcagatgag gtcatcgaag tcgagagcct tctccttacg
      961 cacgatcgct tcgtagcgtg gccacacctc agacaccacg cgcatctgga agctgcgacg
     1021 cccgtctgac tcacggaatt cttctgcggt ccttccctcc cccttctctt ttgagatacg
     1081 cgagagcacg aacccgggtg agagttcctt cgggtcgatg tccatctcct tgagtacctg
     1141 cttcaccgca tcctttgagt cgtcgcgatc gaagatggtg aagtaccgcg gcacgccgat
     1201 gcggctcccg taccgctgga ggatttcgcg gccaagtcca tggaacgtgg ctaccattgg
     1261 aagcggggtg tctccgagcc tgtgtgcgag gcgctctttc atttcgcgag ccgctttgtt
     1321 cgtgaaggta acggccaaaa tgcgctcggc aggggtgccg ttcttgatga ggtggtagat
     1381 acgggtggta agaacgcggg tttttccgga tcctgcgccc gcgagcaccg atataggacc
     1441 atccgtcgca agaactgctt cccgttgcgc gtcgttaagt ccttcgaggt gctgcatgcc
     1501 ttgccaacta tacccgaata tcgctctcaa caccctattt tgagggcggt tgacccctcc
     1561 ctcagagcgt ttatactagc ttcatcgagt gagtggctga cacgcaagat atggcttaga
     1621 tacgccatat ttgtattctg aagaaacgct gaaatacccc tggttcccct atttctgacc
     1681 ctcttaaacc ttccgatccc atcgccttag cgaacgatcg aaatgcatac tggattcctg
     1741 gcgctctaat cgccgcaagc ctccttctgg ggataggaat cgtgcttctg ccgaatgccg
     1801 cgggcgcatt ctggccattt tccgtaacaa acgcgtcaga cggaggttct tccgtgccta
     1861 tggtgcatga cagcagcctc gacctccttg aggcggctac aaacccggac ccgaaccctg
     1921 caaagggccg cggcggcgta tccatgaccg ggggcaccgc ccttattgcc tattctgggc
     1981 ccgagggaac cattgccgat atcgatcggg caagcaataa cggcaagatc tctacgtacg
     2041 tagttcgcga gggcgactcc ctctccggca tcgcggacat gttcgatgtg tccgggaaca
     2101 ccattctctg ggcaaacaac atcggcaaga acgaccttcg ccctggcatg tcgctcatca
     2161 tcctcccggt ttcaggcgtc aggtacacgg tagcttctgg cgacaccctc gcttctattg
     2221 cgaagaagta cagctcagac gcgagcgaaa tcgcctcata caacggtatt tcctcggtag
     2281 acgagctggt gaagggcacc actgtcatca ttccgggagg tgagcttgca agtgcgccgg
     2341 ctgcaaaggc aaagaccgta gcaaagtctt cttcctcagc agcaaagatt aagactggcg
     2401 gttccattgg tagcgtgaag tccaccggta acacgtcaga ctcatccttc tcaggctcgt
     2461 tcacgaatcc ggctccggca ggacgccttt cccagggcat ccatggctgg aacggagttg
     2521 atattgccgc accgtcaggc tcccctattt acgcagccgc agggggtacg gttatcgtct
     2581 cgcgtgtagg tggctggaac ggaggctacg gtaactacgt ggttatcgac cacggaaacg
     2641 gcgtccagac cctgtacgcg catatgagca cggacacggt atcggttggc gagacagtat
     2701 ctcgtggtca gtcgattggt acggtgggca acactggtca gtccaccggc taccatctcc
     2761 acttcgaggt acgcggtgcg aagaatccat tcggatctta gacctaacaa aaaagcaaaa
     2821 gccctccaag cggagggctt tttgctttta agactcttgc tattcaacgg tatcctccac
     2881 agtctcaggc gcttttcctg agagggtgcg gtacgcgtgc gtaaccgcaa ggaacgaaac
     2941 cggaaccgac acgagaagac ccacaacaag cgccagaaga ccgagaatgt tgataccaat
     3001 cagcgcgagc gagaggaaga acagtttcca gcggttcccc ttagtaagct cccagctgcg
     3061 cttgagcgca tcgataggct tcaagtcctg atcgatcacg aggtacccca caaacatgaa
     3121 gaggatgcca aggatgatgc ctgggatgat aagcgcgatg aatccaagga ttatcgcgat
     3181 acctgcaaga atcgatgcgc cgacaaagtt ccagaatggc tttgggtgcc agaggtcttt
     3241 gagtgccgct ttctgtacat cgtcatgcgc cttgagaaag aacgcagtct ctcccatcga
     3301 tacgaagaac gataccgcca taccgaacgc tgccgaagca aggccaacaa ttgctaccac
     3361 tgctccgtct ccgagctcag cgcccgcttc gaacacggac tgcacaaggc ttacgccaag
     3421 gtttatgaga agcaggagta tacctgcctg aacaaaaagc cacggacgcg cacggaaggt
     3481 gttccaaccg tatgagagcg cgcccttaac caagagcgta cccatagact atccgttgag
     3541 gtgtgccttg caggtgcgtg ctgcagggtc tgcttcaaga cgtgcctcct cgatctcttc
     3601 tccagaaacc tcgcagacgc cgtaggttcc ctcttcaaga cgcgcgagtg cggcgaggac
     3661 atcgttgtag cgggtctcaa ggtctacaag aatggcattg ttattaccaa actcctccat
     3721 ctggtctgcg cgatcattcg gatcggcttc gagaccggtc tcttcaggga cggcttccca
     3781 gtcgttcggg ttgcttgggt tcttgcggcc cacggtagcg agctcagcct caagacgggc
     3841 cttctcttcc gtaaggcggg tgcggaaatg ttcggtgttc atgccggtac tctagcatga
     3901 atcgaaccga gaaaacctaa tcccctactc agccttaagt cgcggaagaa gggcttcaaa
     3961 cgctgcttcg tcgcgggcaa taatgagcgt acgtttgtcg gcataaccgt agaggagtaa
     4021 ggaacggccc gaagtatcac gcaggatacg tacatcgtga tttgctacca ccgcatctga
     4081 gaatcgggaa agcgcgcttg caggtacagg atcgagcatt atcggcgtcg aggtcgcggc
     4141 ggtcgaggtt gcggttgtgg atgctaccgg ctcaggagct gctggtattg gatagagcgg
     4201 atacagtacc gaaaggtcgc gtagcatgag tggttcccat gtaagcatgc ctgcataggt
     4261 gcgctcatac gaatcaacac gaagcgcaaa gaatacgctt gactcgctac ctgcgttcac
     4321 gacaccaacc gcgctttcgc gagcgatgtt tcgaaggagg atgtctggag ctggtacctg
     4381 gagcgcacga aggaacgcgg cgccaccggc cggggtcttc acaatacccc cgccctcttc
     4441 ggtcatgtcc tcaaggatgt aggttacgat cacgtttcct gggaccactg cgccgcctgc
     4501 gctcgttgca agcgcgcgca taagctcgcc accggtgcca gagagctctt catattcgtc
     4561 agcaacgatg atcgacggga ccgtg
//
LOCUS       DASWXU010000006         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_522162, whole genome shotgun sequence.
ACCESSION   DASWXU010000006 DASWXU010000000
VERSION     DASWXU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522162"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..2132)
                     /gene="rnr"
                     /locus_tag="VGE48_00155"
     CDS             complement(165..2132)
                     /gene="rnr"
                     /locus_tag="VGE48_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY1041824.1"
                     /translation="MAIQKDTVYEGPITIIKTGKGFFTYDPNEEDMFIAGENLGGAFP
                     GDTVKVQFVGNDTDKRTGKVRPAGRVIEVTERARETFVGKLVENKEENLIMMIPDWKK
                     MYVPFVVKGESLPIGHKVVMRFKGWAAHQPYPWGELEEIIGPAGEHETEMRALALSSG
                     FRSDFPPGVMQEAKYLEEHGRRMLKDGADEGIANGTREDFRGTTTFTIDPFDAKDFDD
                     ALSVKELPNGNYEIGVHIADVSHFVRPGTQIDNEARKRATSVYLVDRTIPMLPHILST
                     DLCSLNPNEDRLAMSAVFELDKEAGIVSRWYGPTVIHSDKRFTYEEAQEVLDKQDGIL
                     LNELNILKTLADKIRTRRTNKGAIAFDTPEVKILLDETGKPTEIVLKERRDTNLLIED
                     FMLLANEAIAEFLTDEMKKARIVGGTIYRVHDQPDADRIENLASFLRVMGYQLPVQAG
                     KVKGEDLNALLKEVAGKPEEYLIKTAALRSMAKAVYSTKNIGHFGLAFEFYTHFTSPI
                     RRYPDLLVHRLAKHYTVEGVFDKSEYAELDQLATRSSEREAAAVEAERDSIKMKQVEY
                     MSEHIGEEFDAVISGVSDRGIFVELTESRAEGMIRVRELGDDYFVYDEKRYRLIGEKS
                     KTEFALGDPIRVRLVDARLAERELDFAIVRK"
     gene            2287..>2524
                     /gene="rpsP"
                     /locus_tag="VGE48_00160"
     CDS             2287..>2524
                     /gene="rpsP"
                     /locus_tag="VGE48_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY1041825.1"
                     /translation="MLMIRFQRIGRKNDAAFRMAVLEKTAGPKAGKYVDLVGTYNPKT
                     KAITYKADRIKDWMSKGAQVSPSLKNLLIKEGVLE"
BASE COUNT          485 a          723 c          747 g          569 t
ORIGIN      
        1 agactgcggt gtacagccat ttggaaaagg aaagaatgca gacggtagcg atgcaatcgc
       61 aatcaacctc cgcacgctcg atggagtcga ctcagagaca ctgaaccgag tcccgtacga
      121 cgggaagaac gcgtaagaga aaaggcgcct tcgggcgcct ttttctattt tcgtacgatc
      181 gcgaagtcga gttctcgttc tgcgagccgc gcatcaacga gacggactcg gatagggtcg
      241 ccgagcgcaa attcggtctt cgacttctct ccaatgagac ggtaccgctt ctcgtcatag
      301 acgaagtagt cgtctccaag ctctcgcacc cggatcatgc cctcggcgcg agattccgta
      361 agctccacga agatgccgcg atcggagacg cccgagataa ccgcatcgaa ctcctcgccg
      421 atgtgctcac tcatgtactc cacctgcttc atcttaatgg agtcgcgctc tgcttctacg
      481 gcagctgctt cgcgctcaga tgagcgggtc gcgagttgat cgagctctgc gtactcgctc
      541 ttatcgaaga cgccttcaac ggtgtagtgc ttcgcgaggc gatgcacgag gaggtccggg
      601 tagcggcgga tcggggaagt gaagtgcgtg tagaactcga aggcgaggcc gaagtgcccg
      661 atgttcttgg tcgagtacac cgccttcgcc atcgaacgga gcgctgcggt cttgatgagg
      721 tattcctcag gcttgcctgc gacttccttt agaagtgcat taagatcctc gcccttaacc
      781 tttccggcct ggaccgggag ctggtatccc atgacgcgca ggaacgaagc aaggttctcg
      841 atgcggtccg cgtccggctg gtcatgcacg cggtatatcg tgccacctac gatacgtgcc
      901 ttcttcatct cgtccgtgag gaactcggcg atcgcctcat tggcgaggag catgaagtcc
      961 tcgatgagga ggttggtatc gcggcgctcc ttgaggacga tctctgtcgg cttaccagtc
     1021 tcgtcgagaa ggatctttac ctcaggcgta tcaaacgcga tggcgccctt gttcgtacgg
     1081 cgcgtgcgga tcttgtccgc gagcgtcttc aggatattga gctcgttcaa gagaatgccg
     1141 tcctgcttgt cgaggacttc ctgcgcttcc tcgtacgtga agcgcttgtc cgagtggatg
     1201 acggtagggc cgtaccagcg ggagacgata cctgcctcct tgtcgagctc gaataccgcg
     1261 gacatcgcga gtcggtcctc gttcgggttg agggagcaga ggtccgtaga gaggatgtgg
     1321 ggaagcatcg ggatggtgcg gtccacgaga tacaccgagg tagcgcgctt cctggcctcg
     1381 ttgtcgatct gggtgcccgg gcgcacgaaa tgagagacgt ccgcgatatg cacgccgatc
     1441 tcgtaattgc cgttcggaag ctccttcacg gagagcgcgt catcgaagtc cttcgcgtcg
     1501 aatgggtcga tagtgaaggt ggtggtgccg cggaagtcct cgcgggtccc gttcgcgatg
     1561 ccttcgtctg cgccgtcctt gagcatcctt cggccatgtt cctcgaggta tttcgcttcc
     1621 tgcatcacgc caggagggaa gtcggagcgg aagccgcttg aaagcgcgag cgcacgcatc
     1681 tcagtctcgt gttctccggc cgggccgatg atctcctcga gctcgcccca tggatacggc
     1741 tgatgcgccg cccaaccctt gaagcgcatc acgaccttgt gcccgatagg gagggactcg
     1801 cccttcacga cgaagggcac atacatcttc ttccagtccg ggatcatcat gatgaggttc
     1861 tcctccttgt tctccacgag ctttccgacg aaggtctcgc gggcgcgctc ggttacctca
     1921 atcacgcgtc cggcagggcg cacctttccg gttcgcttgt cggtgtcgtt cccgacgaac
     1981 tgcaccttta cggtgtctcc ggggaaggcg ccgccgaggt tctctccggc gatgaacata
     2041 tcctcctcgt tcgggtcgta ggtgaagaat cctttcccgg tcttgataat ggtgatggga
     2101 ccctcgtaga cggtgtcctt ctgtatagcc atgtgtagaa ctctagcaga catacctgta
     2161 tttgctatga tttttaggtc gacgttctcc gttgacacag ggtgatgagg actcggggcg
     2221 gctccggccg cccctatttt atttctttga cggaaaagcc gctatttggt acagttccgc
     2281 ctacatatgt tgatgatccg attccagcgc attggccgca agaacgacgc tgcgttccgc
     2341 atggccgtac tcgagaagac ggccggtcct aaggccggca agtacgtcga tctcgtaggt
     2401 acgtacaatc cgaagaccaa ggctattacc tacaaggcag accgcatcaa ggactggatg
     2461 tctaagggcg cccaggtatc cccgtccctc aagaacctcc tcatcaagga gggcgtgctc
     2521 gagg
//
LOCUS       DASWXU010000007         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_424557, whole genome shotgun sequence.
ACCESSION   DASWXU010000007 DASWXU010000000
VERSION     DASWXU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424557"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VGE48_00165"
     CDS             <1..354
                     /locus_tag="VGE48_00165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY1041826.1"
                     /translation="YALALSRQLQAGIDEKKLVDGLVAHLKAEGRMKLLPGIVRELKA
                     LDARRQSLAPSIEVASEAEKASALSSAKALGIEAENISVNPSLIRGWRARSGSSLVDR
                     SAKRGLVDLYQRITN"
     gene            357..1913
                     /gene="atpA"
                     /locus_tag="VGE48_00170"
     CDS             357..1913
                     /gene="atpA"
                     /locus_tag="VGE48_00170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY1041827.1"
                     /translation="MESIIKRIEAEIASFPGTAASAGAEETGIVRATGDGIAEIDGLS
                     NAIMTEMVLFDPTFDRSLEDALKDADPVYGLVLNLEEDGVRAVILGDASKVYEGMLVR
                     RVGRLLSIPVGDSLVGRVVNPLGEAVDGKGPTKANTFRPIEKEAYGVIDRAPVNTPLH
                     TGIKAIDAMTPIGRGQRQLIIGDRGTGKTTIAVDTILNQKSEDSATRPICVYVAIGQK
                     ESKTAKIVAQLAEAGAMDYTIVVTAPASSPAAFQFLAPFAGAAIAEHFMEQGKDALVI
                     YDDLSKQAVAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSKEKGGGSITALP
                     IIETQENDVSAYIPTNVISITDGQIFLETDMFNKGFRPAMNVGISVSRVGSAAQTKAM
                     KKVSGKIKLELAQFRELEAFMQFSSDLDENTKKQIDGGRRMTEILKQGNGKPLPFELQ
                     AAIIFAATNGYFDGFAPEDTAAAEERLQDFLNREATGILAAIRTTREIGETTEKDLRA
                     ALDTFVSRSA"
     gene            1920..2828
                     /gene="atpG"
                     /locus_tag="VGE48_00175"
     CDS             1920..2828
                     /gene="atpG"
                     /locus_tag="VGE48_00175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY1041828.1"
                     /translation="MALKDIKTKIRATERMHKVTRAMEAVSAVKMRKTQAAALQGRAY
                     ARAALSILTRLAGTRHVKEHPLGMDRAGHKLALVVITSDKGLAGALNSGVLKRAVEAL
                     EGREKEDVTIYAYGRKGDEFFSRRGYRVAKAVMNKQDEIPLSDMEELVSELTDGFLEG
                     AFDKVIAVYPNFKSTFEQIPTMRRLLPLSLPAINDIVNGITPVKGKWAETPEIEAPAS
                     YEVEPSGGEVLSLLIPKLVAVALYHMLLEAKASEHSARMVAMKNASDKSKEVTRDLNR
                     VYNKARQAAITREVSEIVGGREALSV"
BASE COUNT          618 a          809 c          851 g          582 t
ORIGIN      
        1 tacgcactcg cgctttcacg gcagcttcaa gcaggtatcg acgagaagaa acttgtcgac
       61 ggccttgttg cgcatctgaa ggcagagggg cgtatgaagc tccttccggg catcgttcgc
      121 gagcttaagg ctcttgatgc tcgtaggcag tcactcgcac cttctatcga ggtagcgagc
      181 gaggctgaga aggcgtccgc gctttcttct gccaaggctc tcggcatcga ggcagagaac
      241 atatcagtga atccgtccct cattcgcggg tggcgcgcac gcagcggaag ctcgctcgta
      301 gaccgttccg caaaaagagg cttagtagac ctttaccaga gaattacgaa ctagttatgg
      361 agagcatcat caagcgcatc gaggcagaaa ttgcctcatt cccaggtacc gcagcatctg
      421 cgggagctga agagaccggc atcgtccgtg cgaccggaga cggtatcgcg gagatcgacg
      481 gactttcgaa cgccatcatg acggagatgg tgctcttcga tcctaccttc gaccgttctc
      541 tcgaggatgc gttgaaggat gcggatccgg tatacggtct cgtgttgaac ctcgaggagg
      601 acggtgttcg tgccgttatt cttggggacg cctccaaggt ctacgaaggc atgttagttc
      661 gccgtgtggg tcgcctccta tccatcccgg taggtgactc attggtagga cgcgttgtga
      721 atccgctcgg cgaggcggtt gacggcaagg gcccgacgaa ggcaaacacc ttccgtccaa
      781 tcgagaagga agcatacggt gttatcgatc gtgctccggt gaacaccccg ctccataccg
      841 gtatcaaggc gatcgatgcc atgactccga ttggccgtgg tcagcgccag ctcatcatcg
      901 gtgaccgtgg tacgggtaag accaccatcg cggtcgacac catcctcaac cagaagtctg
      961 aggactcagc gacgcgtccg atctgcgtgt atgtggctat cggacagaag gagtcgaaga
     1021 ccgcgaagat cgttgcacag cttgcagaag cgggtgcgat ggactacacc atcgtcgtga
     1081 ccgctccagc gtcatccccg gcagcgtttc agttcctcgc gccatttgca ggtgctgcga
     1141 ttgcggagca cttcatggag cagggcaagg atgcgctcgt aatctacgac gacctttcga
     1201 agcaggccgt cgcctaccgc cagctctccc ttcttctccg ccgcccgcca ggacgcgagg
     1261 catacccggg agatgtgttc tacctccact cacgtctcct tgagcgtgcc gcgaagctct
     1321 ctaaggagaa gggtggcggt tctatcaccg cgctcccgat catcgagacg caggagaacg
     1381 acgtgtccgc atacatccca acgaacgtga tttctatcac ggacggccag atattccttg
     1441 agaccgacat gttcaacaag ggcttccgcc cggcaatgaa cgtgggtatc tccgtgtccc
     1501 gcgtgggctc agccgcgcag accaaggcta tgaagaaggt ctcgggcaag atcaagctcg
     1561 agctcgcgca gttccgcgaa cttgaggcgt tcatgcagtt ctcctcggat ctcgatgaga
     1621 acacgaagaa gcagatcgac ggtggtcgcc gcatgacgga gatcttgaag cagggcaacg
     1681 gtaagccgct tccgtttgag cttcaggcag cgattatctt cgccgctacg aacgggtact
     1741 ttgacggctt tgcgccggaa gataccgcag cagctgagga gcgtcttcag gacttcctta
     1801 accgcgaggc taccggcatc cttgccgcta tccgcaccac gcgtgagatc ggcgagacta
     1861 cggagaagga tcttcgtgct gcgctcgata cgttcgtatc ccgctccgca taacgactca
     1921 tggcgcttaa ggacatcaag acgaagatca gggcgaccga gcgcatgcac aaagtgacac
     1981 gcgctatgga ggccgtgtca gctgtaaaga tgcgtaagac gcaggccgca gcccttcagg
     2041 gccgcgcgta tgcgcgtgca gcgctctcaa ttcttacgcg tcttgccgga acgcgtcatg
     2101 tgaaggagca tccgcttggt atggaccgtg cagggcacaa gctcgcgctc gtcgttatca
     2161 cgagcgataa gggccttgct ggcgcgctta actcaggagt tcttaagcgt gcggttgaag
     2221 cgctcgaggg tcgcgagaaa gaggacgtaa ccatctatgc gtatggccgt aagggcgacg
     2281 agttcttcag ccgtcgcggc tatagggttg cgaaggccgt gatgaacaag caggacgaga
     2341 tcccgctttc tgatatggaa gaactcgtgt ctgagcttac cgacggattc ctcgagggag
     2401 ccttcgacaa ggtgatcgcg gtatacccga acttcaagtc caccttcgag cagatcccga
     2461 ccatgcgtcg cctgcttccg ctttcccttc cggcaatcaa cgatatcgtg aacggcatca
     2521 cgccggtgaa gggcaagtgg gcagagacgc ctgagatcga agcgccagct tcgtacgagg
     2581 tggagccgtc cggaggcgag gtgctttcac ttctcatccc gaagctcgtt gcggttgcct
     2641 tgtaccacat gctgctcgag gccaaggcat ccgagcattc ggcacgtatg gtggcgatga
     2701 agaacgcatc agataagtca aaggaagtga cccgcgacct caaccgcgtc tataacaagg
     2761 cgcgtcaggc cgcgattacc cgcgaggtct cggaaatcgt gggcggccgc gaggcgttat
     2821 cagtttaggt actgggcgaa tttataacca aatcattatg
//
LOCUS       DASWXU010000008         6490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_678830, whole genome shotgun sequence.
ACCESSION   DASWXU010000008 DASWXU010000000
VERSION     DASWXU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678830"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..828
                     /locus_tag="VGE48_00180"
     CDS             73..828
                     /locus_tag="VGE48_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041829.1"
                     /translation="MALDWAGKRRFYIIGGAALIALVLLAGILIAVMYEAPSCMDGKQ
                     NQDENGIDCGGSCAYLCSADVEPARVPFARALMNLSGRTDLIAYVENRNALAEAKNAS
                     YVAEIFDAQGRLLGTREGIVDLPARAIVPVFVPGIGAGGAARAFLTIAETTKWRLPSN
                     VKEPLAVGTVSMIQGEKARVTAPVENLSPRDEFDRTVIAVVFNANGEAVAATQTVLRV
                     VSAFGKTDAVFTWNEPFPEGALRAEVRIVPRLP"
     gene            825..1964
                     /locus_tag="VGE48_00185"
     CDS             825..1964
                     /locus_tag="VGE48_00185"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY1041830.1"
                     /translation="MKGLVGKTPRTSWLAEWPLLLIPVALSLLGIVTMHTFGGEGSLA
                     PRQLIWLVIAIGVFVACASADVRFMRRTPVILVGYAISVFLLLLLLVTAYSIQGARSW
                     FDLGFFTLQPSDPIKLVFIALMAKYFSRRHMEIGDFRHIIVSGAYAAGLILLILVEPD
                     LGTAAIFSIVWFGMILVSGISKKHLAIIVTAAAIVISGLWVFALRDYQRDRIVSFLNP
                     AADIHGAGYNAYQATVAVGSGEVTGKGIGYGTQSKLQFLPEYETDFIFASYAEEWGFV
                     GIVLVLLLYGILLARLLAIARRSATNFDAFFTIGVALLFTAHVFIHAGINLGLLPVTG
                     TTIPFMSYGGSHLIVEFAALGIVASLARDARSVPRERAGNEYLGG"
     gene            complement(1961..3124)
                     /locus_tag="VGE48_00190"
     CDS             complement(1961..3124)
                     /locus_tag="VGE48_00190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1041831.1"
                     /translation="MYDRPEVAGPARPLRILYVITKANWGGAQRYVFDLATAAKRAGH
                     DVLVVSGNSGPLTERLEAAGVASESIGSMQRDIKIRAEIDSFLALRTIVRDFGPDVIH
                     GNSSKAGALAAIIGRIERVPHIVFTAHGWAFNESRPKWQKALIAVFHYLTVLLSHNTI
                     AVSKAIQRDARFMPLVGKRMRVIHNGVSPVSFLSRTEARQALVPEFVRSHPDALWIGV
                     IAELHPTKGLDTLIEAFAKVAREDVPVVLVIMGEGQSYAWLSKLAQVFDLPDRIYLAG
                     FVPDAGRYLPALDIFALPSRTEALGYAFLEAGLAKLPCVGSEVGGIPEVLEHEVTGLL
                     VPSNHSKALATALHTLIHDSALRERLGQALEEKVRTKFSIERMIERTLALYRN"
     gene            3148..4506
                     /locus_tag="VGE48_00195"
     CDS             3148..4506
                     /locus_tag="VGE48_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY1041832.1"
                     /translation="MVYGGKRAPALLFIGDIAVFCVSLWLTLFLRTGALPTTALLADH
                     VRPFAILFALWALVFYMSGLYGKGIVLLKSHLPQALLRTQVANILLAALFFFAIPGIG
                     IAPKTNLAIYLVVSLLLIFFWRLALYPRLTVHRERGKAILIAAGEDANLLQEEVNGNP
                     RYPMRFIASWTPASFMALSEAERAQATEGIDLVVADIAACSAHGLLPVLYDLPGGIGT
                     YELVDFTDLYEEVFDRIPLTLLDQSWFLEHVTERDPLWYTVPKRAIDIVGGVAMGLVT
                     LIVLPFVWIALRLEGPGPLLIAQERMGKRGSTMTVYKFRSMAKVDGGAWKGETENRVT
                     KVGSILRQISIDEFPQFWNIIRGDISLIGPRNDLLSLGQRLEEALPYYVMRYAVTPGL
                     TGWAQINQQYEPGNLSPQSIQETTVRLAYDFYYLKHRSLGLDLVIALKTIKRMFFRLS
                     TW"
     gene            4517..5584
                     /locus_tag="VGE48_00200"
     CDS             4517..5584
                     /locus_tag="VGE48_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY1041833.1"
                     /translation="MKHTVFITGAAGYVGTMLVRRFAAREDVERVIGLDKEPVPDLIK
                     DEPKLTYLETNTADEGWEEQVRALNPDIVIHTAWQIREIYGNRPLTWKWNIEGSDRVF
                     DFAYGTPSVKRLVHFSTVASYGAFASNTLEHRFTEDDPFRKTDYLYAEEKRITEEHLK
                     ERFDRASRKVPTAIVRPAAITGPRGRFMRIRFGLQAALAGQLKDSFVYRIVSLMTRFV
                     PVTPKWLRQFIHEDDVVNLVELLALSEAKGDYEVFNICPPGDVVLGKDMAAAVGKKAI
                     MIQPWMARIPFAVLWHGTRGKVPTAKGSWKGYSYPIAVDGSKLTKVYGYEYRYPSLDA
                     FRYTDGEYECFVPEPLRNPKP"
     gene            5581..6408
                     /locus_tag="VGE48_00205"
     CDS             5581..6408
                     /locus_tag="VGE48_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY1041834.1"
                     /translation="MIVDGRSIASDILREVKAGIDRTVVVRAVVVSPSPATESYLSIK
                     AARAEEAGMHLEVVRLDGHATEAEITEWVTSPGCDAVIVQLPLPDHMDQNAVLDAIPV
                     ERDADVLSQEAYARFVQGDGVVPPVASAIAQILIREGIDVSGKRAVVVGQGKLVGKPA
                     AVLLTALGATVETVTKESGDLSLLKDADIVVTGAGVPGLIRPEHLKEGVVLIDAGTSE
                     SDGAIVGDADPACMAVASVFTPVPGGVGPVAVACLFRNVSALLGTPLQDAPKGVDSE"
BASE COUNT         1274 a         1763 c         1969 g         1484 t
ORIGIN      
        1 gcaggagccg tccgaaagga cggtttttgc ttttttatac ctacgctgct tttcctatat
       61 actggtacta ctatggcgct tgattgggca gggaagcgaa ggttctacat cattggcggt
      121 gcggcgctta tcgcgctcgt attgctcgcg gggattctga ttgcggttat gtacgaggcg
      181 ccctcgtgca tggacgggaa gcagaatcag gatgagaacg ggatagactg cggcggctca
      241 tgtgcgtatc tctgtagcgc agacgtcgag cctgcgcgcg taccatttgc gcgtgcgctc
      301 atgaatctct ccggacgtac cgacctcatc gcatacgtag agaaccgcaa cgcgcttgcg
      361 gaggcaaaga acgcttcgta tgtggccgag atattcgacg cgcagggacg cctgctcggg
      421 acgcgcgaag gcatcgtgga tcttccggcg cgggcaatcg ttccggtatt tgttccggga
      481 atcggcgcag ggggcgctgc gcgcgcgttt cttacgatcg cagagactac gaagtggcgg
      541 ttgccaagca atgtgaagga gccgcttgcg gtaggcaccg tatcgatgat ccagggagag
      601 aaggcgcggg tcacggcgcc ggtagagaac ctgagtccac gggacgagtt cgataggacg
      661 gttatcgccg tcgtgttcaa tgccaatgga gaagcggttg ctgcgacgca gaccgtgctt
      721 cgcgtcgtat ccgcgttcgg aaagaccgat gcggtgttca cttggaacga accatttcct
      781 gagggtgccc tacgcgctga ggtacgcatc gtgccgcggc taccatgaag ggactcgtag
      841 ggaagacacc aaggacctct tggcttgccg aatggccgct tctgttgata ccggtggcgc
      901 tctcgctgct tggtatcgtc acgatgcaca cgttcggggg tgaaggctcg ctcgcgccga
      961 ggcagctcat ctggcttgta atcgcgattg gggtattcgt tgcgtgtgcg tctgcggacg
     1021 tgcgattcat gcggcgtaca cccgtgattc tggtggggta cgcgatatcg gtgttcctcc
     1081 ttcttttgct tctggtgacg gcgtattcga tccagggtgc tcgctcgtgg ttcgatctcg
     1141 gcttcttcac gctccagccg tcggacccaa tcaagcttgt gttcatagcg ctcatggcga
     1201 agtacttctc caggcgtcac atggagattg gggatttccg gcacatcatc gtctcaggtg
     1261 cgtatgccgc aggactcatc cttctcattc ttgttgagcc ggatctcggt acggcagcga
     1321 tcttcagtat cgtatggttc ggcatgatcc ttgtgtctgg tatctcgaag aagcaccttg
     1381 cgattatcgt taccgcagcc gccatcgtta tctcagggct ctgggtgttc gcgctccgcg
     1441 attaccaaag ggaccgtatc gtgtccttcc ttaaccctgc cgctgatatc catggagcag
     1501 ggtacaacgc gtaccaggct acggttgcgg tgggttctgg agaggtaact gggaagggca
     1561 tcggatacgg gacgcagtct aagttgcagt tcctccctga gtatgagacc gactttatct
     1621 tcgcgtccta tgccgaagag tggggatttg tcggcatcgt gctcgtactt ttgctttacg
     1681 gcatcctgct tgctcggttg cttgcgattg cgcgtcgatc ggcgacgaac ttcgacgcgt
     1741 tctttacgat tggcgtggcg ctccttttca cagcacacgt gttcatccat gcgggcatca
     1801 acctgggact acttccagtg acgggtacta ctattccgtt catgagctac ggcggatcac
     1861 acctcattgt tgagttcgcg gcgctcggca tcgtcgcgtc acttgcgcgt gatgcgcgca
     1921 gcgtgccaag ggagcgcgct gggaacgagt atctgggcgg ctagttccga tagagcgcaa
     1981 gagttcgctc tatcatgcgc tcaattgaga acttggtccg taccttctct tcaagcgcct
     2041 ggccaagacg ctctcgcagt gcgctgtcgt gtatgagcgt gtgaagcgcg gtagcaagtg
     2101 cctttgaatg gttcgagggt actagaaggc ccgtcacctc atgctcaagc acttcaggaa
     2161 tgccgcccac ctcgcttccc acacacggga gctttgcaag tcccgcttca aggaatgcgt
     2221 acccgagagc ctctgtgcga gacgggagtg caaagatgtc gagcgcagga agataccgtc
     2281 cggcgtccgg gacaaagccg gcgaggtaga tgcgatcagg aagatcgaac acctgagcga
     2341 gttttgagag ccaggcatac gactggcctt cacccatgat gacgagcacc accggtacgt
     2401 cctcacgcgc tacttttgcg aacgcttcga taagcgtatc gagtcctttt gtaggatgga
     2461 gctctgcgat gacgccgatc cagagcgcgt ccgggtggct acgcacgaat tccggaacga
     2521 gtgcttgtcg cgcttccgta cgagaaagaa atgagacagg ggagacgcca ttatggatga
     2581 cccgcatgcg ctttccgaca agcggcataa aacgcgcatc gcgctgtatg gctttcgaga
     2641 ccgcaatggt gttatgcgag aggagaacgg ttaggtagtg gaagacggcg atgagtgcct
     2701 tctgccactt cggccgagac tcgttgaatg cccagccatg cgccgtgaat acgatatgcg
     2761 gcacgcgctc gatgcgaccg atgattgcgg cgagcgcccc agccttcgag ctgttcccgt
     2821 ggatcacatc cgggccgaaa tcacgcacga tggtgcggag cgcgaggaac gagtcgatct
     2881 cggcccgaat cttaatgtcg cgctgcatgc tgccgatcga ttcggaagcc acacccgccg
     2941 cctcaagccg ctcggtcagg ggacccgagt ttccgcttac cacgagcacg tcgtgtccgg
     3001 cccgcttcgc agcggtcgct aggtcaaaga cgtagcgctg cgcaccgccc cagttagcct
     3061 tggtaataac gtagaggata cggagcggcc gagcggggcc tgctacttcc gggcggtcat
     3121 acatgttcta tagtataaag gaagctcatg gtatatggcg gcaagcgcgc cccggcgctc
     3181 ctcttcatcg gcgatatcgc cgtattttgc gtttccctct ggctaacgct cttcctgcgt
     3241 actggagctc tcccgacaac cgcacttctt gcagatcacg tgcgaccgtt tgcgatcctg
     3301 ttcgcgctct gggcgctcgt gttttatatg tccggcctct atggaaaggg catcgtgctt
     3361 ctcaagagtc atctcccgca ggccctcctc aggacccagg tagcgaacat tcttcttgca
     3421 gcactcttct tctttgcaat tcctgggata ggaatcgcgc cgaagacgaa cctcgcgatc
     3481 tatctcgtgg tctcgcttct tcttatattc ttctggcgcc ttgcactcta tcctcgtctt
     3541 acggtgcatc gagagcgagg gaaggcgatc cttatcgctg caggcgaaga tgcgaatttg
     3601 ctgcaagagg aagttaatgg gaacccacgc tatccgatgc gcttcatagc ttcttggacg
     3661 cccgcgtcgt tcatggcgct ttctgaggcg gaacgtgctc aagcgaccga gggtatcgat
     3721 ctcgtagttg cggacattgc tgcatgcagc gcgcacggac tccttccggt gttgtatgac
     3781 cttcctggag ggataggtac ctatgagctc gtggacttca cggatctcta tgaggaggtg
     3841 ttcgaccgta tcccgctcac acttttggat caaagctggt tcctggaaca tgtcacggag
     3901 cgtgatccgc tctggtacac cgttcctaag cgcgcaatcg acatcgtcgg aggtgtcgcc
     3961 atgggactcg taactctcat agtacttccg ttcgtatgga tcgcgcttag gctcgaggga
     4021 ccagggccgc ttcttatcgc gcaggagcgt atgggtaagc gcggctctac catgacggtt
     4081 tacaagttcc gcagcatggc gaaagtggat ggtggcgctt ggaagggaga gaccgagaac
     4141 cgtgttacga aagtgggatc gatcctgcgc cagatctcga tagacgagtt cccgcaattc
     4201 tggaacatca ttcgtggaga catctcgctc attggtcctc gtaacgacct cttgtcactc
     4261 ggacagcggc tcgaggaggc gcttccgtat tatgtgatgc gctacgccgt tacacctggt
     4321 ctcaccgggt gggcgcagat caatcagcag tacgagccgg ggaatctctc gccgcagtcg
     4381 atacaggaga ccacggtgcg cctcgcgtac gatttctact acttgaagca ccgctcgctc
     4441 ggccttgatc tcgtgatcgc gctcaagacc atcaagagga tgttcttcag gctttcgacc
     4501 tggtagtctc atttatatga agcacacggt attcatcacg ggcgcggcag ggtacgttgg
     4561 tacgatgctc gtgcgcaggt tcgcggcgcg cgaggatgtc gagcgtgtga tcggccttga
     4621 taaagagccg gtgccagacc ttatcaagga cgagccgaag cttacctacc tagagacgaa
     4681 tactgcagac gaagggtggg aggagcaggt tcgggcgctt aaccctgaca ttgtcatcca
     4741 tactgcgtgg cagatccgcg agatctacgg caaccgtccg cttacctgga agtggaatat
     4801 cgagggctcg gaccgcgtat tcgatttcgc gtacggcaca ccgagcgtga agcgcctcgt
     4861 acacttctcg accgttgcct cgtacggcgc tttcgcatcg aacacgctcg agcatcgttt
     4921 taccgaggac gacccattcc gaaagaccga ctacctgtac gcggaagaga agcgcataac
     4981 cgaagagcat cttaaggagc gctttgatag agcatcgcgc aaggttccaa ctgcaattgt
     5041 gcgtcctgca gctatcacgg gtcctcgtgg tcgcttcatg cgtatccgct tcgggctcca
     5101 ggcagcgctc gccggacagc tcaaagactc gttcgtatac cgcatcgtgt ctcttatgac
     5161 acgctttgta ccggttacgc cgaagtggct tcgtcagttc atccatgagg atgatgtggt
     5221 gaacctcgtc gagttgctcg cgctttctga agcaaaggga gactacgagg tcttcaatat
     5281 ctgccctccg ggagacgtcg tactcggcaa ggatatggca gcagccgtag ggaagaaggc
     5341 gatcatgata cagccgtgga tggctcgtat accgttcgca gtactctggc acggaacgcg
     5401 cggcaaggtt cctacggcca aagggagctg gaaggggtac tcgtacccga ttgcggtaga
     5461 tgggtctaag ctcacgaagg tatacggata cgaatatcga tacccgagcc tcgacgcatt
     5521 ccgctatacg gacggagagt atgagtgctt cgtgcctgag ccgctgcgca atccgaagcc
     5581 atgatcgtag acggacgcag cattgcttcc gacatcttga gggaagtgaa ggcaggcatc
     5641 gatcgcaccg tcgtggtgcg tgccgtcgtg gtatctccgt ccccggcaac tgagtcctat
     5701 ctctcaatca aggcagcgcg tgctgaggaa gctgggatgc atcttgaggt agtgagactc
     5761 gatggtcatg ctacggaagc ggagatcacg gagtgggtga cgtcccctgg atgcgacgct
     5821 gttatcgtgc agctgccgct tccggaccac atggatcaga atgcagtact tgatgccatt
     5881 cccgttgaac gggatgcaga cgtgctttcg caggaagcgt acgcgcgttt cgtgcaaggc
     5941 gacggagtag tgccgccggt cgcctcagcc atagcacaaa tccttatacg tgaaggaatc
     6001 gatgtgtccg gaaagagggc ggtcgtagta gggcagggta aactcgtagg gaagcctgca
     6061 gcggtgctcc ttacggcgct cggcgctacc gttgagacgg taactaagga atcaggggac
     6121 ctgtcgcttc tgaaggatgc ggacatcgtg gtcacgggcg cgggagtgcc ggggctcata
     6181 cgtccggagc atttgaagga aggggtggtg cttattgatg caggcacctc agaatcggac
     6241 ggtgctatcg taggggatgc ggatccggcg tgtatggcgg tagcgtccgt gttcacgccc
     6301 gttccagggg gcgtcggtcc ggtcgcggtt gcatgcctat ttcgcaatgt aagcgccctt
     6361 ctgggaaccc cgttgcagga cgctccaaag ggcgtagact ctgagtaata catcatttat
     6421 atgcgctaga tacctgctct cgtggtgctc gtcgtgtctg gcattctcgg ctggtacgtt
     6481 ctctctccgg
//
LOCUS       DASWXU010000009         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_731785, whole genome shotgun sequence.
ACCESSION   DASWXU010000009 DASWXU010000000
VERSION     DASWXU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731785"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1322
                     /locus_tag="VGE48_00210"
     CDS             285..1322
                     /locus_tag="VGE48_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041835.1"
                     /translation="MPVVLRHRGDLSVHDIERLAREQLVRVTPHVGNWTPARLALPQL
                     GVSILCVDHTIGERDVNAHPHLLIRGGSAELLSEHGDRLTTHAYVSSDAPNIPFTQGT
                     SLSAVHVGAIRSLYPGTEIITHVEHMRENHELLLTVLEIVTALSRTATWWRRVDRHGM
                     VTAYRGKDIPRTWDAILPDIFPIMNPEEGWLVHNRVSILFDLIQQSRMGQEPEIYHLS
                     GPDMVRYLGQEIETVSRMYDTVRARLNLKQETVTYNLVPFASFRFATRTAQASACERL
                     CESLMRNEPILLRECVRETSDVLAQSETKSYFTQHDCHAQGERIVLCDTARAWSMDTC
                     AAYLACVRAAA"
     gene            1431..1946
                     /locus_tag="VGE48_00215"
     CDS             1431..1946
                     /locus_tag="VGE48_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY1041836.1"
                     /translation="MQQLHPRAIWLFFFNNVVLGAGAFLALAFACVGFVLARADDGVA
                     FDFALPIMLVVFAAVVYLLAAYGWALLTVRFYRYELREDGFRKEHGVIWKKYITIPYD
                     RIQNVDIHRGILARLLGLSDLQIQTAGAITAGSYGAFSEGRLPGIDRALAEEIRDQLV
                     ARVRGGTTQGL"
     gene            complement(1943..2674)
                     /locus_tag="VGE48_00220"
     CDS             complement(1943..2674)
                     /locus_tag="VGE48_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY1041837.1"
                     /translation="MQIRIVVANIQSGIGVTKGYRQYLTSSWRYILPHDGPGALGAGA
                     FLRAEDADIALLTEVDAGSRRSRGISQIDAIALEAGLSHARFFPTRSVGTSINEGNAV
                     LSRFPILSTRAHPLRSGVNPRVLGEAILDADGTPLTVLVAHLALGTRLRESQIAEIAA
                     RVKDVNGPLILGGDFNERDQSAFRLLLRAGLASVAVPSYPSWKPRHALQMLFHTRDIR
                     IESAAVPPDSRFSDHLPLIVAATLD"
     gene            2785..2857
                     /locus_tag="VGE48_00225"
     tRNA            2785..2857
                     /locus_tag="VGE48_00225"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2818..2820,aa:Thr,seq:ggt)
     gene            3231..>3520
                     /locus_tag="VGE48_00230"
     CDS             3231..>3520
                     /locus_tag="VGE48_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041838.1"
                     /translation="MQFIRTSLENLTIPRLLPLLFTVLYTAFAAFHFVSIGNSEFLGY
                     IAVIVLLVVLGGCILSHQCVPAWLLWLLSSVGLLHMLGAAMLVNGDVLYNYVP"
BASE COUNT          746 a          999 c          973 g          802 t
ORIGIN      
        1 tccgtacccg ctttacctgg acgacttccc gaggaaggcg cgtgtatgct gttgcgtaag
       61 taagaaactc caattgcaaa agcacccgtc ttcctcggaa gacgggtgct tttcttctct
      121 ctatgacttt tccctcacac ctctcaaata ctgcatggcg ccgctttcgt cgcgtatttg
      181 ctaccgaact tcggtggtcg ctttgtagta cttgatgtaa gagatccgat caagaacgca
      241 caggggccac ggaaggcccc gttgttcttt gaaaggagat catcatgcct gtagtgcttc
      301 gccatcgagg cgacctgtca gtacacgaca tcgagcgtct ggctcgcgag cagctcgtac
      361 gagtcacgcc gcatgtcggc aattggacgc cagctcggct cgcgctccct caattgggag
      421 tatcgatcct gtgcgtcgac cacacgatcg gagagcggga tgtgaacgcg cacccgcacc
      481 tcctaattcg gggtgggagc gcagagctgc tttcagagca cggagaccgt ctcacgactc
      541 atgcatatgt atcaagtgat gctccaaaca ttcccttcac tcaaggcaca tcgctttctg
      601 cggtgcatgt tggagcgatc cggtctctct atccagggac tgagatcatc acgcacgtcg
      661 agcatatgcg tgagaaccat gaactgcttc ttacggtgct tgagatcgtt acggcgctct
      721 cgcgcacggc aacgtggtgg aggagggtgg accgacacgg gatggtcacc gcgtatcggg
      781 ggaaagatat ccctcggaca tgggacgcta tcctgccgga catcttcccg atcatgaatc
      841 cagaggaagg gtggctggta cacaaccggg tatccatcct gttcgatctc atccagcagt
      901 ctcggatggg tcaggagccc gagatctacc atctctcagg tcctgatatg gttcggtatc
      961 tcgggcagga gatcgagacc gtgtctcgca tgtacgacac ggtgcgagcg cgactcaacc
     1021 tcaagcaaga gaccgtcaca tacaacctgg taccgttcgc atcgttccgg ttcgcaacca
     1081 gaacggcgca ggcatcagca tgtgaacgcc tttgcgaatc gctcatgcgc aacgaaccaa
     1141 tacttctcag agagtgtgta cgtgagacat ccgacgttct cgcgcaatca gaaaccaagt
     1201 cgtacttcac acagcatgat tgtcatgcgc aaggagaaag gatcgtactc tgcgataccg
     1261 cacgtgcttg gtctatggac acctgtgcag cgtatcttgc gtgtgtacga gcagctgcat
     1321 gaaccttcct gtaaccgccc ccagtttggg gcggttttct tttgcgtatg tgcatacgct
     1381 tcctatatct cttcacgtac tgctcagcag taaatgctag acttacctat atgcaacaac
     1441 ttcatcctcg ggcgatctgg ctcttctttt tcaacaatgt ggtgctcggt gcaggagcat
     1501 tccttgcact cgcatttgct tgtgttggat tcgtccttgc acgtgcggat gacggcgtag
     1561 cattcgactt cgcgctcccg atcatgctcg tggtcttcgc agcggtcgtg tatttgcttg
     1621 cagcgtatgg gtgggcgctg ctcacagtac gtttctaccg ctacgagctg cgggaagacg
     1681 ggtttcgaaa agagcacggt gtgatctgga agaagtacat caccatcccg tacgaccgca
     1741 ttcagaatgt cgatattcat cgcggcatcc tcgcacgctt gcttggtctc tcggatctcc
     1801 agatacagac tgcaggcgct attacggcag gaagttatgg cgcgttctct gagggcagac
     1861 tccctggtat agaccgtgcg cttgctgaag agattcgaga tcagcttgtt gcgcgagtgc
     1921 gaggcggaac aacgcaagga ctttaatcga gtgttgccgc tacgataagc ggaaggtggt
     1981 cagagaaccg cgaatccggg ggcacggccg cgctctcaat cctgatatcc ctcgtgtgaa
     2041 agagcatttg gagtgcatgg cgtggcttcc acgagggata actcggcacc gccacagagg
     2101 cgaggccagc tcgcagtaat agcctgaagg cgctctgatc ccgctcatta aagtctccgc
     2161 caagaatgag cggcccattc acgtctttta ctcgtgcggc aatttccgcg atctgcgact
     2221 cgcgcaggcg cgtgccaagc gctaggtgcg ccacaagcac ggtgaggggt gtaccgtctg
     2281 cgtcgagaat cgcttctccg agcactcttg gattcacacc actgcgtaat ggatgcgctc
     2341 tggtactgag gattgggaag cgggacaaca ccgcgtttcc ctcatttata gaagtaccga
     2401 cgcttcttgt aggaaagaag cgggcgtgcg agagccctgc ctcaagagcg atagcatcga
     2461 tctgggagat gccgcgggat ctgcgtgaac ccgcatcaac ttctgtgaga agcgcgatat
     2521 ccgcgtcctc agcacgtaga aatgcccctg cgccgagcgc cccggggccg tcatggggga
     2581 gaatatagcg ccagctgctc gtgaggtact gccggtaccc cttagtcacg ccaatgcccg
     2641 attggatgtt cgctacgact atccgtatct gcattccgta cattgtacaa agtggtttcg
     2701 aaacggagcg tgaagcagcc ctggcctcgg ttgaaaaccg cagagaaatg ccgtactctc
     2761 aggaagtccg cttcggcgga cattgccgat gtagctcagt cggtagagcg cgtccatggt
     2821 aaggacgagg tccccggttc aatcccgggc atcggctccg acacagtgca taccgccgct
     2881 ttgcaaaagg gcgaataggt gcaagagtga tggcttcgca gcaatgcgaa gcctgacctt
     2941 ttcaaccaga tcctaaggaa ggatttctga tgaccgagac gacaaaccgc agggacgggt
     3001 ctgacccgac gggacagcac tccgtcggcg agatccagcc aaggaccacg ggccgtgtgc
     3061 gtccggacga ctatcagccg gcctcgcgca gacaacccgg cgacgacctc taccaggacg
     3121 acagtcatcc tcgggacgat tgaccacaag cagaagcttt cgagggcgcg gaccataccg
     3181 cgccctttag ctttatgaac ccttcatata ggagtcggta tcttcacgtc atgcaattca
     3241 tacgaacctc gcttgagaac ctcactatcc cgcgcctcct cccgcttctt tttacggtcc
     3301 tctacacagc attcgccgcg ttccactttg tctccatagg gaatagcgag tttctcggat
     3361 acatagccgt tatcgtgcta ctcgttgtcc tcggcggctg tattctttcg catcagtgcg
     3421 ttccggcatg gctcttgtgg ctcttatcct ccgtagggct cctgcacatg cttggggcag
     3481 cgatgcttgt taatggcgac gtgctgtaca actacgtgcc
//
LOCUS       DASWXU010000010         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_880407, whole genome shotgun sequence.
ACCESSION   DASWXU010000010 DASWXU010000000
VERSION     DASWXU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880407"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="rplB"
                     /locus_tag="VGE48_00235"
     CDS             <1..831
                     /gene="rplB"
                     /locus_tag="VGE48_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY1041839.1"
                     /translation="KPTTKSRRHMTVVTYRGVLSGDKPYKALLSTKKNQAGRNNAGRI
                     TMRHQGGGHKRRLRDVDFKLDKRDIPARIETIEYDPYRTAFIARTVFADGERRYVTVP
                     KDMKVGDTFLISEKAPVKTGNRLPLGKIPAGTFVYNVELRPGAGARIARSAGNYAEIV
                     AHDAGYAQLKMPSSEIRKVIESAWASVGTVGNEEQRLQNYGKAGKSRWKGIRPTVRGT
                     AMNPVDHPHGGGEGVQGRGLRRAKSMWGKPTGKGQKTRTPKKYSNKFIVSRRKVGKKR
                     "
     gene            complement(916..1257)
                     /locus_tag="VGE48_00240"
     CDS             complement(916..1257)
                     /locus_tag="VGE48_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041840.1"
                     /translation="MSQNKTCKRVFQAISRTLAQLDDAMGTDLDEVARHIRMHAKFET
                     LSRKVYKSLVIEATEISNLVREVVVKITRVSGHFQLEESDSSQEIVHEIEQTIRLENQ
                     HGRWTRVSAYG"
     gene            1347..1973
                     /locus_tag="VGE48_00245"
     CDS             1347..1973
                     /locus_tag="VGE48_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041841.1"
                     /translation="MKLPLAVLSVCALFLAASAGVFVYMSAPKAAPEALVEEVPMVDV
                     TATTSEPVATLPEEPRCTLGVPGPASSPLTDVITPAMKDAAVDAVIAREKLFLNADAD
                     CLRAYSLAVAVSPEQKEEIAKQDDKTIRATALMMALIMDLPEEKEVRRLMLKPDAEWK
                     YKEGEVSVSVTIDNPPEEVVGHSDTGEPVYGTSNGWGTTIYYRNGRWY"
     gene            complement(1970..2785)
                     /locus_tag="VGE48_00250"
     CDS             complement(1970..2785)
                     /locus_tag="VGE48_00250"
                     /inference="COORDINATES: protein motif:HMM:NF023166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HEY1041842.1"
                     /translation="MIDLTSPSARRAELLGAAALITIILGFTLWYMATHQAPEPYSPV
                     PETEEVAESLPPETYEEHGQYFDIEATYPAETPLKATAGEDADTAAVALMEDFVDETI
                     ADFKRQGNFDNLTPEDVQIMGLSDTRKESLTLIYEERMGDDTITYVFTLHVDTLGAHP
                     NTFYRSFTFDRVSGEELKIQDLFVSGSGYLTRLSAIAEFELSKILGEFADLDYIRQGV
                     AAEAINFQSYAIEGKDLILIFPPYQVAPYAAGTQTVAIPLAQLSEILRPEYRP"
     gene            complement(2837..>3191)
                     /locus_tag="VGE48_00255"
     CDS             complement(2837..>3191)
                     /locus_tag="VGE48_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041843.1"
                     /translation="DFQRFLEDRVPLEEKPRVPNICEQILDRLAKRFPRLHQEVLASE
                     VLRKAQESLLQALRRRENVAIERLAGGHFREIRENEKHELLETVNQASGVWVFMFAHD
                     NLYADSPGPCVLCYM"
BASE COUNT          699 a          877 c          954 g          661 t
ORIGIN      
        1 aagccaacca cgaagtcccg tcgtcacatg accgtcgtca cgtaccgtgg cgtactctcc
       61 ggtgacaagc cgtacaaggc gcttctttcc acgaagaaga accaggccgg ccgcaacaac
      121 gcaggacgca tcaccatgcg ccatcagggc ggtggtcata agcgccgtct ccgcgatgtc
      181 gacttcaagc tcgacaagcg cgacatcccg gcacgcatcg agaccatcga gtacgatccg
      241 taccgcaccg cgttcatcgc gcgtaccgtg ttcgcagacg gcgagcgccg ctacgtaacg
      301 gttcctaagg acatgaaggt aggggacacc ttcctcatct ccgagaaggc accggtaaag
      361 accggtaacc gccttccact cggcaagatc cctgcaggta ccttcgtgta caacgtagag
      421 ctccgcccgg gtgcaggcgc acgcatcgcg cgttccgccg gcaactacgc tgagatcgtt
      481 gcgcatgacg caggctacgc gcagctcaag atgccttctt cggagatccg caaggttatc
      541 gagtccgcat gggcatccgt cggtaccgtc ggcaacgagg agcagcgtct ccagaactac
      601 ggtaaggcag gtaagagccg ctggaagggt attcgcccaa ccgtccgcgg tactgccatg
      661 aacccggtag atcacccaca cggaggtggt gagggcgttc agggccgcgg tctccgacgt
      721 gccaagagca tgtggggtaa gccaactggc aagggccaga agacccgtac tccaaagaag
      781 tactcgaaca aatttatcgt tagccgccgc aaggtcggaa agaagcgata ggagaaggcc
      841 ccgcgagagc ggggcttttt ctttgggtag agcgaagggg tccccaagcg ggaacccctc
      901 atcggctgcg ttctgctagc cgtacgcact cacgcgagtc cagcgaccat gctggttttc
      961 caaacgaatc gtttgttcga tttcgtggac gatttcctgc gatgagtcgg attcttcgag
     1021 ttggaagtgt cctgagactc gggtgatctt caccacgacc tcccgcacca gattcgagat
     1081 ctccgtggcc tcgatgacga ggctcttgta gaccttcctc gagagcgtct cgaacttcgc
     1141 gtgcatccgg atgtgacggg cgacctcgtc gagatcggtc cccatggcat catccagctg
     1201 cgcgagcgtt cgggagatcg cttggaagac ccgcttgcag gtcttgttct ggctcatgac
     1261 tgtaacccct ctctgttgtg caacctgaaa tcgtttatag cagaaactta tctgtgtgtc
     1321 gcgcaattag gtgatacgct acctgcatga agcttccgct cgccgtgctc tcggtgtgcg
     1381 cactttttct tgctgcgtct gcgggggtct tcgtatacat gagtgcgcca aaagcagcgc
     1441 cagaagcact cgttgaagaa gttccgatgg tagacgttac tgctaccact tcagaaccag
     1501 tagcaacgct ccctgaggaa ccacggtgca cgctcggtgt tccgggacca gcgtcctcgc
     1561 cgcttacgga cgtaataaca cccgcgatga aagatgcggc agtagatgct gttattgctc
     1621 gagagaagct ctttctgaac gcagacgctg attgtctgcg cgcctacagt cttgcggttg
     1681 cggtaagtcc ggagcagaag gaagagatcg cgaaacagga cgataagacc atccgggcta
     1741 ccgcgcttat gatggcgctc attatggacc ttcccgagga gaaggaggta cgacgtttga
     1801 tgctcaagcc ggatgctgag tggaagtaca aggaaggcga ggtgagtgtt tccgttacaa
     1861 tcgataaccc tcctgaggaa gtggtggggc acagcgatac cggagaaccc gtttacggca
     1921 ccagcaacgg ctggggcacc acgatctact accggaacgg gcggtggtat taggggcgat
     1981 attcaggcct caagatctcc gaaagctgcg caagagggat cgcaacagtc tgcgtacctg
     2041 ctgcgtatgg cgctacctgg tacggcggga atatgaggat gagatccttg ccctcaatag
     2101 cgtagctctg gaagtttatg gcctcagcgg ctacgccctg gcggatgtag tcgaggtcgg
     2161 cgaactcgcc gaggatcttc gagagctcga actcagcgat tgcagagagg cgcgtaaggt
     2221 atccggaacc ggagacgaaa aggtcctgga tcttgagctc ctcgcctgag acccggtcga
     2281 aggtgaagga gcggtagaag gtgttcgggt gcgcgccaag cgtgtccacg tggagcgtaa
     2341 acacgtaggt gatggtgtcg tctcccatgc gctcctcata gatgagagta agggactctt
     2401 tgcgggtgtc tgagagaccc atgatctgca cgtcctcagg ggtgaggttg tcgaaattgc
     2461 cctggcgctt gaagtccgcg atagtctcgt ccacgaagtc ctccatgagc gctacggccg
     2521 cggtgtccgc atcttcgcct gcggttgcct tcagtggggt ctcggcaggg taggtggcct
     2581 caatatcgaa gtactggccg tgctcttcgt aggtctctgg cgggagtgac tcagctacct
     2641 cttcggtctc aggaaccggg gaatacggct caggagcctg gtgcgtggcc atataccaga
     2701 gcgtaaagcc gagaatgatg gttatgaggg ctgcagcacc gagaagttct gcacgtctag
     2761 cagacgggga ggtgagatcg atcatatgag gaaagtatag cgtaaggaat aggccccgcc
     2821 ggagcggggc cacaaatcac atatagcaga ggacgcaggg tccaggtgaa tcggcgtaca
     2881 ggttgtcatg tgcaaacatg aatacccaca cccccgatgc ctgattaacg gtttctaaga
     2941 gctcatgctt ctcgttttct cgtatttccc gaaaatgtcc gcctgccagg cgttcaattg
     3001 caacattttc tcggcggcga agcgcttgaa gcagggattc ttgagcctta cgaagaacct
     3061 cgctcgctaa cacctcttgg tgcaatcggg gaaacctttt tgcgagccga tcaaggattt
     3121 gctcacagat gttgggaacc cttggctttt cctccaacgg gacccggtct tctaagaagc
     3181 gctggaaatc c
//
LOCUS       DASWXU010000011         6408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_472481, whole genome shotgun sequence.
ACCESSION   DASWXU010000011 DASWXU010000000
VERSION     DASWXU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472481"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..428)
                     /locus_tag="VGE48_00260"
     CDS             complement(282..428)
                     /locus_tag="VGE48_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041844.1"
                     /translation="MADADELERDADREEERAGAHTESAELLRKQAEEQREDQRREEE
                     AHED"
     gene            complement(497..883)
                     /gene="msrB"
                     /locus_tag="VGE48_00265"
     CDS             complement(497..883)
                     /gene="msrB"
                     /locus_tag="VGE48_00265"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY1041845.1"
                     /translation="MEKTEEEWKETLKPEEYRVLRESGTEAPFVGKYVNNHEDGTYRC
                     RACGVVLFSSDTKFDSGSGWPSFTDPAVAENVGTREDESHGMRRTEVFCRNCGSHLGH
                     VFDDGPKEAGGKRYCINSIALDFEKQ"
     gene            950..1402
                     /locus_tag="VGE48_00270"
     CDS             950..1402
                     /locus_tag="VGE48_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY1041846.1"
                     /translation="MNRTTLLAIVIALAFLGMADSWYLFQSAVTDTALTCDIGGALDG
                     CNIVAQSPYSQFLGLPLALYGVGFFALIFALALGIFVLPKRILFGALYALSWFGAAAS
                     LVFVFIQFALIKALCIYCLFSAVIAFLVLAAAHVLWTRYAPVKLIPVL"
     gene            1440..2603
                     /locus_tag="VGE48_00275"
     CDS             1440..2603
                     /locus_tag="VGE48_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041847.1"
                     /translation="MACFRFLAVILLIGTLFPAEASAATFWNARSLVISESPEGNAYV
                     AGTDVAVVAPLSADLVGAAGTLTVAGPVEGDVLAAGGTVTIERAVQGDVRALGAQVSV
                     TSPVTGDLVLAGGTVVASSSAAETRILGGTVRMNGGGAKTVIYGSDIQLSGIFEGDVE
                     VLATDRLTLQEGTVISGELRYDAPQEADIPSTATVSGGVFYTGASSYLPSVEEAQTFA
                     IAGAGVFLIVRIISLLIGAALLAGLFPQFSQRVADRTLARSPGKFALLALLGFAVVFA
                     APVLIILLLISFVGMAVAFLLLVAYLLLLMLGYLYAGVIAGAALGRGLLKQEQVTWKL
                     AILGMLVLCLVGMVPVIGKLILFLLFLAATGAIVAIAYRFAFSRTDALETETL"
     gene            2605..3072
                     /gene="bcp"
                     /locus_tag="VGE48_00280"
     CDS             2605..3072
                     /gene="bcp"
                     /locus_tag="VGE48_00280"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY1041848.1"
                     /translation="MLPSPGTSAPDFTLLDQDGVSHTLSAHKGTRVLLYFYPKDDTPG
                     CTKQACALRDAMPDLAKLDAIVFGISPDSVKSHKKFAEKYGLPFTLLADEGHRVADAY
                     GVWGRKKFMGREYDGVFRTSFLIGRDGNIEIVFENVKPEKHADEVLVYLKSHA"
     gene            3065..3736
                     /gene="nth"
                     /locus_tag="VGE48_00285"
     CDS             3065..3736
                     /gene="nth"
                     /locus_tag="VGE48_00285"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY1041849.1"
                     /translation="MPKVSTYEERKARAKKILSYLKKTYPVPETELNYKTPFQLVTAV
                     MLSAQSTDKRVNLVTETLFKQYKSVNDFADADLATFTEQISSITFYQNKARHILATAK
                     IVRDQFKGRVPKTEVELVKLPGIAYKTAHVILGELHDVWEGIPTDTHVRRFALKFDLT
                     DRTELTKISKDLEELIPKKDWKYVNNGLVLYGRYVCPALPHACENHPLTKLWPPAAGR
                     WPKAK"
     gene            complement(3792..4238)
                     /locus_tag="VGE48_00290"
     CDS             complement(3792..4238)
                     /locus_tag="VGE48_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041850.1"
                     /translation="MTATLAGVANDDARADTRLVEALKRHGFDATLEDAGFCDGDVSA
                     PCIRVFKFRGEGEHSRLTELVLEFYESTEVPMLDRDTQLRVNAFDKTVDGELEFDYEL
                     SFDGLETLVLMPREIFEIHPDSKRSIEERYFAKIERFADFLENRDP"
     gene            4344..4592
                     /locus_tag="VGE48_00295"
     CDS             4344..4592
                     /locus_tag="VGE48_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY1041851.1"
                     /translation="MEKKLYRSRTNRMIAGVMGGLGEFFGVDPALLRLGYLILTVFTG
                     FVPGIVGYVLAIIIVPEATLVTPAEPVAEKGSDDAEAV"
     gene            4573..5382
                     /locus_tag="VGE48_00300"
     CDS             4573..5382
                     /locus_tag="VGE48_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY1041852.1"
                     /translation="MTPKRFRNLVYAWYEKEGRTHLPWRKTRDPYRILVSEIMLQQTQ
                     VERVIPFYESFLESFPDVKALASAPLSRVLQKWQGLGYNRRAKMLHEAAKVAVREHKG
                     KIPKDVFALESLPGIGHYTARAVATFSWNQDVVFVETNLRTAVIHHFFSTKEGVRDAE
                     VLAILEKTLPKGEARNWYAALMDYGSYLKRSGVRINAKSASYTKQAAFRGSGREARGA
                     ILRALARKPEKETYLIGILGPDREEQVKEQLQKLLKEGLVEKARGMYRLPS"
     gene            complement(5379..>6408)
                     /locus_tag="VGE48_00305"
     CDS             complement(5379..>6408)
                     /locus_tag="VGE48_00305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1041853.1"
                     /translation="SLGALITLCVFPFVPRLVRRYSSRTLAITFAFSQAVLLSVLAAA
                     PAPVFAIICIALIFATAPFISYQLDLLLECASTDESSAGRVRTLFITAASLAYVVAPL
                     LVAFLIDGTDAYWKIFLAAALSLTPFIALLIFEKLPEGEPPELHAMLATCSCLWKDKD
                     LRAATLAGAVLQFFFHLAPLYVPLYLHTVLGFPWDELGWIFALMLVPFLFVEYPAGYI
                     ADRWIGDKELLITGIVIMGAAFAAIGFIIATTPLFVIAAILFATRIGAALVEAMTEGH
                     FFRRVSGKDATTISLFRMTRPFGALVAPLFASVLLLTGNFVLFFLVSGALIVALGIVF
                     TIPLKDVR"
BASE COUNT         1425 a         1759 c         1820 g         1404 t
ORIGIN      
        1 agcacccttg aggaatgcta cggccgtacg ttctatttcg gtaagaactc cagggaggag
       61 gagcttgagg acgatgatgg cgaccccgag tccgatggct ttcgtgaatg ccataggcgt
      121 atgtgtgaac gaataaatac acactggacg gggaagagtg ctgggcaccc ttgccgaatc
      181 atcctttcaa agtcctttcc ctctgcccat tagaacctgt acgaagtccc taaggttaca
      241 cgaaaccgcc cggggtttcc ggacggtttg ttagctcaag gctagtcttc gtgcgcttcc
      301 tcttcgcgcc tttggtcctc gcgctgttcc tcagcctgtt tgcggaggag ttctgcgctc
      361 tcagtgtgcg cgccagcccg ctcctcttcg cgatccgcat cacgctcaag ctcatctgca
      421 tcagccataa gaaatatcag ggttacctgc taggaacccc atacttgcag atagcacatg
      481 gagcattcat gaagcgctac tgcttctcga aatcaagcgc gatggagttg atgcagtacc
      541 gcttgccgcc cgcctccttg gggccgtcgt caaatacgtg acccagatga ctcccgcagt
      601 ttctgcagaa gacttcagta cggcgcatgc cgtgactctc atcctccctg gtgcccacgt
      661 tttccgctac ggcagggtcc gtgaatgaag gccagccgct gcctgaatca aacttggtgt
      721 cggaggaaaa gagcaccaca ccgcatgctc ggcagcgata ggtgccgtcc tcgtggttgt
      781 tcacgtactt tcccacaaat ggggcttcgg tcccggattc ccggagcacc ctatattctt
      841 cgggcttcaa cgtctctttc cactcctctt cggtcttttc catataccca tactagcgcc
      901 tttgcatgaa ggttgccggt acgggcggag gcagggtagg attatgttca tgaaccgcac
      961 taccttgctt gcgattgtaa tcgcactcgc gtttcttggc atggcggact cctggtacct
     1021 ctttcagagt gcggtaactg ataccgcact cacctgtgat atcggcggcg cgcttgatgg
     1081 ttgtaacatc gtggcacaga gtccgtactc acagttcctc ggactcccgc ttgcgcttta
     1141 tggcgtcggg ttctttgcgc tcatattcgc gctcgcgctc ggcatattcg tattgccgaa
     1201 gcgcatactc tttggtgctc tgtacgcgct ctcctggttc ggagctgcgg catcgctcgt
     1261 atttgtattc atacagttcg cacttatcaa ggcgctttgt atctactgtc tcttttcggc
     1321 agttatcgcg ttccttgtcc tcgctgcagc ccatgtcctc tggacccggt acgcgcccgt
     1381 aaagctcatt ccggtgctct aggttgacgc atgggccctc gtggtatgat ggcggcatca
     1441 tggcctgctt cagattcctc gcggtcattc tcctcatagg cacgcttttc ccagcggaag
     1501 cgtcagcagc gaccttctgg aatgcacggt ctcttgtcat atccgagtcc ccggaaggca
     1561 acgcctacgt cgcaggtact gacgttgcgg tggtcgcacc gctctcggcg gacctcgttg
     1621 gtgccgcggg cacgctcacg gtagccggcc ctgtcgaggg tgacgttctt gctgcaggag
     1681 gcactgttac catcgagcgc gcggttcaag gcgatgttcg cgcgctcggt gcacaggtat
     1741 cggtaaccag tcccgtgacg ggagaccttg tcctggcagg aggaacggtc gtagcttcgt
     1801 cctcagccgc agagacgcgc attcttggcg gcacagtgcg catgaacggg ggcggcgcaa
     1861 agaccgttat ctacggatca gatatccagc tctctggaat ctttgaggga gatgttgagg
     1921 tactcgcaac cgaccgactt acgcttcaag agggtacggt tatctcgggc gagcttcggt
     1981 atgacgcacc gcaggaagca gatattcctt caacggctac ggtttcggga ggcgtctttt
     2041 ataccggtgc gtcctcatac cttccaagcg tggaggaggc gcagaccttt gcgattgcag
     2101 gcgcaggggt attcctcatc gtgcgcatca tatcgctcct catcggcgca gcgctgcttg
     2161 cgggtctttt cccgcagttt tcacagcgag tcgcggaccg aacgctcgcg cgttctccag
     2221 gcaagtttgc gctcttggcg ctccttgggt ttgcggtggt gttcgcggca ccggtactta
     2281 tcatcttgct tctaatctcg ttcgtgggaa tggcggtagc gttccttctg cttgtcgcgt
     2341 atctactgct tctcatgctc gggtacctgt acgcgggcgt gattgcgggt gcagcactcg
     2401 gtagagggct cttgaagcag gagcaggtga cctggaagct tgcgatcctc ggcatgcttg
     2461 tactctgtct tgtcggtatg gtgccagtga ttggcaagct catactgttc ctcttgttcc
     2521 ttgccgctac cggagctatc gtcgctatcg cataccgctt cgcgttctcg cgcactgatg
     2581 cgctcgagac agagactctc tagtatgctc ccgtctcccg gaacatccgc gcctgatttc
     2641 acgctcctcg accaggatgg tgtatcgcac acactctcgg cgcacaaagg aacacgcgtc
     2701 ctactctatt tctatccaaa agacgataca ccaggctgca caaagcaggc gtgcgcgttg
     2761 cgtgatgcga tgccggatct agctaagctc gacgccatcg tgttcggcat ctcgccggac
     2821 tcggtgaaga gccataagaa gttcgcggag aagtacgggc tcccattcac gctgcttgcc
     2881 gacgaaggac atagggtagc tgacgcgtac ggcgtctggg gcaggaagaa gtttatgggg
     2941 cgtgagtatg atggtgtatt ccgcacctcg ttccttatcg gccgagacgg gaacatcgag
     3001 atcgtattcg agaatgtgaa gccggaaaag catgccgacg aggtgctcgt gtacctcaag
     3061 agtcatgcct aaggtctcaa cctacgaaga gcgcaaggca cgcgccaaaa agatactttc
     3121 atatctcaag aaaacctatc cagttcctga gacggagctc aactataaaa ccccgttcca
     3181 gctcgttacg gccgtgatgt tgtcagcgca gtctaccgac aagcgcgtga acctcgtaac
     3241 cgagactttg tttaagcagt acaaaagcgt gaatgatttc gcagatgcgg acctagcgac
     3301 cttcacggag cagatatcat cgataacgtt ctatcagaac aaggctcggc atatcctcgc
     3361 aaccgcgaaa atagtccggg atcaattcaa gggaagggta ccgaagaccg aggtcgagct
     3421 cgtgaagctc ccgggaatcg cgtacaagac cgcgcatgtg atcctgggag agctccatga
     3481 cgtatgggaa ggaatcccta cggatactca cgtccggaga ttcgcgctca agttcgacct
     3541 caccgaccga actgagctca cgaagatatc aaaggatctg gaggagctca ttcctaagaa
     3601 ggattggaaa tacgtgaaca acggcctcgt gctctatggt cggtacgtgt gtccggcgct
     3661 cccacatgcg tgcgagaatc atccgcttac gaagctctgg ccgcctgcgg cagggaggtg
     3721 gccgaaggca aaatgaacaa gggccgcatc gtaccgaaag ggtacgaggc ggcccaagtg
     3781 acgacgaagc gtcagggatc gcggttttca aggaaatcgg cgaatcgctc gatcttcgcg
     3841 aaatagcgct cctcgatgct gcgtttgctg tccgggtgga tttcgaagat ctcgcgcggc
     3901 atgagcacga gggtctcgag gccgtcgaag gagagctcgt agtcgaattc gagctcgccg
     3961 tcgacggtct tgtcgaacgc gttcacccgc agctgcgtgt cgcgatcgag catcgggact
     4021 tcagtggact catagaactc gagcacgagt tcggtgaggc gcgaatgctc tccctcaccc
     4081 cggaacttga atacccggat gcagggcgcc gacacgtctc cgtcgcagaa ccctgcgtct
     4141 tccagggttg cgtcgaatcc atgtcgcttc agcgcctcca ccagtcttgt gtcggcgcgt
     4201 gcgtcgtcat tggcgactcc agcgagtgtc gcggtcatac ttttctccct ccgagatagg
     4261 aacaatctcc caaaaatatt attgcaatta aaaatataaa agtcaataaa tccctgcacg
     4321 gtaaaggggt atactagaca catatggaaa agaaactcta caggagccgc accaatcgca
     4381 tgattgccgg cgtcatgggc ggactcggtg aattcttcgg ggttgaccca gcactccttc
     4441 gtctcggtta cctcatcctt acagtcttca ctggcttcgt tccaggaatc gtcggatacg
     4501 tgctcgcgat cataatcgtc cctgaagcca ccctcgtaac gccggctgaa ccggttgcag
     4561 agaaggggag cgatgacgcc gaagcggttt aggaatctcg tatacgcttg gtacgagaag
     4621 gagggcagga cacatctgcc gtggcgtaag acgcgtgatc cgtaccggat ccttgtatca
     4681 gagatcatgc ttcagcaaac gcaggtagag cgggtaatcc cgttctacga atcgtttctc
     4741 gaatcgtttc ctgacgttaa ggcgctcgca agcgcaccgc tttcacgcgt actgcagaag
     4801 tggcaggggc tcgggtataa ccgcagggcg aagatgctgc acgaagcagc aaaggtcgcg
     4861 gttcgcgaac acaagggaaa aattccgaaa gatgtttttg ctctcgaatc cctgccgggt
     4921 atcgggcact acactgctcg cgcggttgcc acgttctcct ggaaccaaga cgtggtattc
     4981 gtagagacga atctgcggac cgcggtgata caccatttct tttctaccaa ggaaggggtg
     5041 cgggatgccg aagtgctcgc gatacttgag aagacgcttc caaaaggcga ggcacgcaac
     5101 tggtacgcgg cgctcatgga ttacggctca tacctcaagc gctcgggcgt acgcattaac
     5161 gcaaagagcg cgagctatac gaagcaagct gcgttccgcg gttcaggtcg cgaggcgcgc
     5221 ggcgcaatac tacgagcgct tgcgaggaag ccagagaaag aaacctatct aataggtatt
     5281 ctcgggccgg atcgagagga acaggtaaaa gaacaactac agaaactcct gaaggaaggc
     5341 ctcgtagaga aggcgcgcgg aatgtatcgg ctcccgagct agcgcacgtc cttgagcggt
     5401 atcgtgaata cgatgccaag cgcaactatg agtgcgcccg agacaaggaa gaagagcacg
     5461 aagtttccgg tcagaaggag cacactcgcg aacaggggag cgacaagcgc accaaacgga
     5521 cgtgtcatac ggaatagact tatcgtagtc gcatctttac ccgagactct cctgaaaaaa
     5581 tgtccttcgg tcatggcttc tacaagcgcc gcgccgatac gcgttgcaaa cagaattgct
     5641 gcgataacaa agagtggcgt agttgctatg atgaatccaa tagcggcaaa tgccgcgccc
     5701 atgataacga tgccggtgat aagaagttcc ttgtcgccga tccagcggtc tgcaatgtat
     5761 cctgcagggt attccacaaa caggaacggc acgagcataa gcgcgaatat ccagccgagc
     5821 tcgtcccagg ggaatccgag gaccgtatgg agatagagcg gaacatacaa gggcgcaagg
     5881 tggaagaaga actgcagtac tgctcctgcg agtgtcgcag ccctcaggtc cttgtctttc
     5941 cagaggcacg aacaggtagc aagcatcgcg tgcagttccg gaggttcgcc ctcaggaagc
     6001 ttctcgaata tgagaagcgc tatgaaggga gtaagggaga gcgctgccgc taggaaaatc
     6061 ttccagtacg catcagtacc atcaatgaga aatgctacca agagcggagc aaccacatac
     6121 gcgaggcttg cagccgtaat gaatagcgtg cggacccttc cagcagagct ttcgtccgtg
     6181 ctcgcgcact caagaagaag atcaagttga tacgaaataa atggcgcggt agcgaatatg
     6241 agcgcgatac agatgatcgc aaacaccggt gcaggcgctg cagcaagtac ggaaaggagt
     6301 accgcctgcg agaacgcgaa tgttatggcg agcgtccgtg atgaatacct gcgcacaagt
     6361 cggggtacga acgggaacac acagagggtg atgagcgcgc cgagagat
//
LOCUS       DASWXU010000012         4999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_627830, whole genome shotgun sequence.
ACCESSION   DASWXU010000012 DASWXU010000000
VERSION     DASWXU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627830"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGE48_00310"
     CDS             complement(<1..308)
                     /locus_tag="VGE48_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HEY1041854.1"
                     /translation="MASFDFSPLDKNIKETEEWLVRELSSIRTGRATPAILDNVKPEA
                     YGARQAVNQMAAITIEDARTLRIVPWDKSLTKNIERAIQDADLGVGLATDDQGLRVTF
                     P"
     gene            complement(331..1020)
                     /locus_tag="VGE48_00315"
     CDS             complement(331..1020)
                     /locus_tag="VGE48_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041855.1"
                     /translation="MAHHAYLYVGGKEDGIEAVRAYAARELGLEGVEHPDIAIFSYDG
                     LFSVDDARAVNRFALQAPIAGDRKLVAIQTGRIYHEAQNALLKTFEEPQEGTTLVLIV
                     PTEGIVLPTLRSRLVPFPGRKRTVSKEAQALISASKAEREKLVTKLLARAKSDKDLEK
                     QAARMEAARIIEGLLVAAYEARAGAAGQSAKELDLLLSDLSRFMPIMHQSSAPLKLIF
                     EHLLLVLPAKL"
     gene            1077..1535
                     /locus_tag="VGE48_00320"
     CDS             1077..1535
                     /locus_tag="VGE48_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041856.1"
                     /translation="MNRNVVLGIVVVIALAVLGWWIFAFMMPTPGTPLNTETTSEDVT
                     SPTAEEPFSLSGTWRSTQDPRFVRVFAADGSVTDYYEGEEGATTMGRWNVVEDANAEQ
                     PEFATLSGMRTIKIEFPEEVLYFAVVTLTDEKLELSYLARGNTLSFTRER"
     gene            complement(1575..2585)
                     /locus_tag="VGE48_00325"
     CDS             complement(1575..2585)
                     /locus_tag="VGE48_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY1041857.1"
                     /translation="MPLFSPRIGIDLGTTNVLVFVPGKGVVLNEPSVVAVSTDTNKVL
                     AIGAEAKAMVGRTPDSIQAYRPMKDGVIADYRVTEAMLRYFIRKALGRNLFKPTVLVS
                     VPAGVTSTEKRAVIEAAVKAGASDAYVVKEPILAAIGAGIPIQEALGRMVADIGGGTI
                     DVAVISLGGIVASTSVKVAGNKLDRAIIDYVKKQYNLSIGDKTAEEIKITIGSAVPVT
                     EELVMSVKGRDLVSGLPRTIELKTNEVVQAMNRELREMMNAVRKVLQDTPPELASDII
                     DNGIILTGGTSQLRHLPELVYRRTGVQAKLADDAYYCVARGTGIALNHLDTYRKSIIS
                     KK"
     gene            complement(2663..3157)
                     /locus_tag="VGE48_00330"
     CDS             complement(2663..3157)
                     /locus_tag="VGE48_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041858.1"
                     /translation="MDAFEPFVRLRLGTCRYPETLAMILRERGYKHTLLNRIRTGDIV
                     NLQEEIEVDLVACTNHQLGMETSGWYHQARDAGLALDLELCPQETFLQLLLQHEDSIL
                     GLCERHRRITVSVMSDPISDHRDSYVMQFRVYEGRVLIEPRSLRYGKQYTWQGPFVFM
                     KPRG"
     gene            3256..4566
                     /locus_tag="VGE48_00335"
     CDS             3256..4566
                     /locus_tag="VGE48_00335"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY1041859.1"
                     /translation="MTQSGENNFVVEGGHPLSGSITTNCSKNGAVAMLAASLLNKGTT
                     TLRKVPKIEEVHRLIEVLRSIGVSVEWEGKDVVITPPETIALGDIDRAAAVRTRSILM
                     FIGSLVHLFPSFEIPQSGGCTLGLRSVRSHLWALEKFGISIETLTDHYQVSHDGLSAA
                     EIVLYESSDTATINALLAAARIEGTSVIKYASANYQVQEVCGFLRELGVTIEGLGTTT
                     LTVTGVGQIRKDVSYAIGEDPIDAMFFIALAATTNSALTIRNAPIEFLEVELEVLSKM
                     GLNFSLTDHAISDNGITKLADIHIEPSELTAFPEKIHARPYPGLNIDNLPFFAVIAMK
                     AEGETLIHDWVYEKRAIYYTELDRLGANTLLLDPHRIQLHGPVHLRGAEIVAPPALRP
                     ATILLIGMLAADGRSTLRNVYSINRGYEDLVNRLKSIGAQIEHA"
     gene            4589..4879
                     /locus_tag="VGE48_00340"
     CDS             4589..4879
                     /locus_tag="VGE48_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041860.1"
                     /translation="MKSIGDILPKDFGDQKPSRKTERGELMRFFVRHLNYSRAEDGLP
                     KLTMARMGRILEGIPTDDLYYLKTVCSRAKNFSKKFWWEIDPEKHKEPDTPF"
BASE COUNT         1031 a         1472 c         1428 g         1068 t
ORIGIN      
        1 gggaacgtga cacggagccc ctgatcatcg gtggcaagac ccacgccgag atcggcgtcc
       61 tggatggcgc gctcaatgtt ctttgtgagc gacttgtccc acgggacgat gcggagcgta
      121 cgtgcgtctt cgatggtaat agctgccatc tggttcacgg cctgtcgagc gccatacgcc
      181 tcaggcttca cgttatcgag aatagccggc gtcgcgcggc cggtgcggat cgaagagagc
      241 tcgcgcacga gccattcctc ggtctccttg atgttcttgt cgagcggtga gaaatcgaat
      301 gatgccataa tgctccaaag tgtagcactt ctagagtttc gcgggcagta cgagaaggag
      361 gtgctcgaat ataagcttca gaggtgcaga gctctgatgc atgatcggca tgaagcggct
      421 gagatctgag agaagcaggt cgagttcctt ggcgctctgt cctgcagccc ctgcgcgtgc
      481 ctcataggcg gcaacgagga gcccttcgat gatgcgtgcc gcctccatgc gagcagcctg
      541 cttttcaagg tccttgtcgg acttcgcgcg agccaatagc ttcgtcacca acttctcgcg
      601 ttcggctttc gaagccgaga tgagtgcctg tgcctcctta gatacggttc gcttccttcc
      661 tgggaatggc accagccttg aacggagcgt cggaagcacg ataccctcag tcggtacgat
      721 gaggacgagc gtggtcccct cttgcggctc ctcgaaggtc ttgaggagcg cgttctgcgc
      781 ttcgtgatag atgcggccgg tttgaatcgc gacgagcttt cgatcgcctg caattggtgc
      841 ctggagtgca aatcggttca ccgcacgcgc atcatccacg gagaataagc cgtcgtagga
      901 gaagatcgca atgtcaggat gctcgacacc ctcgagtccg agctcgcgcg ctgcgtacgc
      961 gcgaacggct tcaataccgt cttccttccc tcctacatag agatacgcat gatgcgccat
     1021 atccgtagtc taccccggtg ctagactagg ctccatacga gctagcccat gagcctatga
     1081 acaggaatgt cgttttaggg atcgtagtcg taattgcact tgccgttctc gggtggtgga
     1141 tcttcgcgtt catgatgcct acgccgggca ccccactgaa tacggagacc acctccgagg
     1201 atgtgacgag cccgaccgcg gaagagccgt tctcgctttc agggacctgg cgcagcacgc
     1261 aggacccccg gttcgtccgc gtcttcgctg cagacgggtc cgtgaccgat tactatgaag
     1321 gcgaagaggg cgctacaacc atgggccgct ggaacgtagt cgaggacgcg aacgccgagc
     1381 aacctgagtt tgcgacgctc tccggcatgc gcaccataaa gatagagttc cctgaggagg
     1441 ttctgtactt cgcggtggtg acgctcaccg acgagaaact cgagttgtca taccttgcgc
     1501 gcggaaatac actttcgttc acgcgggaac gctagcaaat agaatgctaa aagcctcgga
     1561 attctccgag gcttttactt ctttgagata atgctctttc ggtaggtatc caaatggttg
     1621 agcgcgatac cagtgcctcg cgcaacgcag tagtacgcgt catcggcgag ctttgcctgg
     1681 actccggtgc ggcggtacac gagctccgga aggtggcgga gctgcgaggt tcctcctgta
     1741 aggatgatgc cgttgtcgat tatgtctgag gcgagttcgg gcggcgtgtc ctgaagcacc
     1801 ttgcgcaccg cattcatcat ttctcttagc tcgcggttca tggcctgtac gacttcgttg
     1861 gtcttgagct cgatggtgcg cggaagtccc gagacgaggt ccctgccctt cacactcatg
     1921 acgagttcct cggtcacggg aaccgcggat ccgatcgtga tcttgatctc ttctgcggtc
     1981 ttgtcgccga tggagaggtt gtactgtttc ttcacgtagt cgatgatggc gcgatcaagt
     2041 ttgtttcctg cgaccttcac agacgtggac gccacgatgc caccgagaga aattacggcc
     2101 acgtcgatgg tgccgccgcc aatatcagcg accatgcgac cgagtgcctc ctggatcggg
     2161 atacccgcgc cgatagcggc gagaatcggc tccttcacga catacgcgtc agacgcgccc
     2221 gccttcacgg ccgcttcgat cacggcgcgc ttctcagtcg aggttacgcc cgctggtacc
     2281 gatacgagta cagttggctt gaagagattg cgacccagcg ccttccggat gaagtaacgg
     2341 agcatcgctt ccgtgacgcg gtaatccgca atcacgccgt ccttcatcgg gcggtacgct
     2401 tggatggaat ccggggtacg tcccaccatc gccttggctt ccgcaccgat agcaagaacc
     2461 ttgtttgtgt cggttgatac cgccactacg gacggctcgt tgaggaccac cccctttcct
     2521 ggcacaaaca cgagtacatt tgtggtccca aggtctatcc cgatgcgcgg agaaaagaga
     2581 ggcatgtggc ccgtatcata cctcgctttc cataaggctt caaaaagccc gaccagggat
     2641 ctggccgggc tttcctgcgg attcacccgc ggggcttcat gaacacgaag ggaccctgcc
     2701 aggtgtactg tttgccatac cgcaacgacc tcggctcaat cagtaccctg ccctcgtaga
     2761 ccctgaactg catgacatag gaatctctat gatcactgat cgggtcggac atgacgctga
     2821 cggtgatgcg gcggtgccgc tcacacaggc ccaggatcga gtcctcatgc tgcaacagga
     2881 gctgcaggaa ggtctcttgg ggacacagct cgaggtcgag cgcgagtcct gcatcgcgag
     2941 cctgatggta ccaaccggat gtttccatgc cgagctggtg gttagtgcag gcgacgaggt
     3001 cgacctcgat ctcctcttgc aggttcacga tgtcgccggt gcgaatgcgg ttgagaagcg
     3061 tgtgcttgta gccgcgctcg cgcagaatca tcgcgagtgt ctcggggtag cgacacgtcc
     3121 ccaggcgcaa gcgcacgaag ggttcgaaag catccatggc attcctccaa agaagtctgt
     3181 ttggacctta cgcctgcctg aggttgctgt aaaggcagat atcaggaacg tgtctcggct
     3241 gttacaatag cggccatgac tcaatccgga gagaacaact tcgtcgtaga gggtggccat
     3301 ccgctctcgg gctctattac aaccaactgc tcgaagaacg gtgccgtagc gatgctcgcg
     3361 gcctccctac tcaacaaggg caccaccacg ctccgcaagg tgccgaagat cgaggaggtg
     3421 caccgcctca tcgaagtact ccgttccatc ggcgtgtcgg tggaatggga gggcaaggac
     3481 gtggttatca cgccgccgga gacgatcgca ctcggagata tagaccgcgc tgcggcagta
     3541 cgcacacgct ccatcctcat gttcattggt tctctcgtac acctcttccc ctccttcgag
     3601 atcccgcaat ctgggggctg caccttgggc ctgcgttccg tgcgctcgca tctctgggcg
     3661 ctcgagaagt tcggcatatc aatcgagacc ctcaccgacc attaccaggt ctcgcatgac
     3721 gggctctcag ctgcggagat cgtgctctac gaatcgagcg acaccgcgac tattaatgcg
     3781 ctccttgcag cagcgcgcat cgaaggaacc tcggttatca agtacgcgag cgcgaactac
     3841 caggtgcagg aggtatgcgg gttccttcga gagctcggcg taaccatcga aggactcggc
     3901 acgactaccc tcaccgtgac cggcgtcggg cagatccgga aagatgtgtc ctacgcaatc
     3961 ggtgaggatc caatcgatgc gatgttcttc attgcgcttg cggcaaccac aaactcggcg
     4021 ctcaccatcc ggaacgcgcc gattgagttc cttgaagtag agcttgaagt gctctcgaag
     4081 atgggactca acttctccct tacggatcat gctatttccg acaacggtat tacaaagctc
     4141 gcggacatcc atatcgagcc ttcagagctc acggcgttcc cggagaagat ccatgcgcgc
     4201 ccgtacccag gcctcaacat cgacaacctg ccgttcttcg cggttatcgc catgaaggcc
     4261 gagggcgaga ccctgatcca tgactgggtg tacgagaagc gcgctatcta ctacaccgag
     4321 ctcgatcgct tgggcgcgaa cacgcttctc ctcgatccgc atcgtatcca gctccatgga
     4381 ccggtacacc ttcgcggtgc tgagattgtg gcgcctcctg cgcttcgtcc tgcaaccatc
     4441 ctccttatcg gcatgcttgc cgcggacggg cgctcgacgc tcaggaatgt ctacagtatc
     4501 aatcgcggat acgaggacct cgtgaacagg ctcaagtcta tcggcgctca gatagagcac
     4561 gcatagtcac gtttgctaca ctgtcgccat gaagagcatc ggcgacatcc tccctaagga
     4621 tttcggcgac cagaagccga gcaggaagac cgagcgcggc gaactgatgc gctttttcgt
     4681 caggcacctc aactactcgc gcgccgagga cggactcccg aaactcacta tggcgcgtat
     4741 gggccgcatc ctcgaaggca ttcccacgga cgacctctac tatctaaaga cggtatgcag
     4801 ccgcgcgaag aatttcagca aaaagttctg gtgggagatt gatcctgaga agcacaaaga
     4861 gccagacacg ccgttctagg agttaattga cagaatctct atttctgata taaaaacatc
     4921 gtgcctcgga gccctgtctc cttcgcacac aagcgttgcc cgcttggcgt tcccgaggga
     4981 cctcccagtg actagtgcg
//
LOCUS       DASWXU010000013        10472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_777203, whole genome shotgun sequence.
ACCESSION   DASWXU010000013 DASWXU010000000
VERSION     DASWXU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777203"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VGE48_00345"
     CDS             <1..585
                     /locus_tag="VGE48_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1041861.1"
                     /translation="FDWNKGFKFSTYATWWIRQAITRALADQSRTIRIPVHMVETIAK
                     YKQVSRRLAQVLGRDPQPEEIAIEMAVEVEKIYQIEKINQDTLSLENPIGSDDDDRST
                     LGDFIADDKIPSPIQESSERILGEQVRDILEDLSPKERKILEMRHGLLDGVYHTLEEV
                     GKEFGVTRERIRQIEAKALEKIRTHEKSRRLKSY"
     gene            complement(676..1014)
                     /locus_tag="VGE48_00350"
     CDS             complement(676..1014)
                     /locus_tag="VGE48_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041862.1"
                     /translation="MQHSGGSIVEDTQYEGVIVSIDPERNFGFARSPNFPVDVYFRLD
                     EGQRILFDPGRNEIIGCIDAQPETPIEVQKVIVFRAKEVDKKLRATHWGMKRDFVHAC
                     RAAGIKPRNS"
     gene            1082..3922
                     /locus_tag="VGE48_00355"
     CDS             1082..3922
                     /locus_tag="VGE48_00355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY1041863.1"
                     /translation="MTDQTAFLEAYTRLNEAQKKAVDTVEGPVLVVAGPGTGKTHILT
                     LRIANILRTTTDTSPENILVLTFTDSAARTVSRRLGTFIGDEAARKVNVFTFHGFAGE
                     ILRRHPEAFPEFADQRLMGEVEQILVWRAVLENATDSKLRTPKSPFHYLKDLKSLEDS
                     LTRECVSTDDYRAWLDREEQSLKDDPKLVYTKGDRAGDLNAAGQKRAERLIKGREAAD
                     LIDAYRTLKEELGVYGYTDILRIVTDALKEDEALRAELQERYQYVLADEHQDANALQH
                     ALLDALAFDEHPNLFIVGDEKQAVYGFQGADTTHFETFTSLYPRTTVIPLTENYRSFQ
                     DILDLSHGLLADLPSSTGGHVELSGFRGKGGTAHILAATDALAERDQIATLVQEAISE
                     GVPPHEIAIITLKNRTADLFALHLRARGIAALRAGDIDLEGRPAIRFLLSLMDAAADP
                     NAPHALREALLAPWWQAPLEERARFLRTHGDWEIEESLKEAFPEIAVIVAGLREKVLV
                     VPPLNAFSYILSDSGARSYFLSSGETVHEDLPLVRQLMTHIEELATRDPNASFKEVME
                     SFGKAREHEVGAIKTSRTQQEGLVTVITAHKAKGMEFERVFVTALSAREWEGRGRSSL
                     VPSPFSASREREELIRLFYVALTRAKDRLVLSFPRESADGKEEAPMSLLPQGLPEIRV
                     SKDPLPKLHAVVDAPKLVCELTLAYLTHEGLSPSAFNEYLESPATFFAKRVLRLREPE
                     NRSIVLGNAVHAAIAAYLRAKDASEEEREAAAYAELTRSFRKSLLPRGDTFAALERQA
                     RTYLTAYLSSDLLGREAVAVEETFRLERTVGEQNILLKGKVDAVFKGDTGECIVDFKT
                     SSTIDKKDQEKFERQIAFYDLLLRNNGHQTEDGLIIQVGGEITEYPVALNDETRASFE
                     ACLKDVLEELISGKWREGEPSPYDDLLGLLGE"
     gene            3937..4779
                     /locus_tag="VGE48_00360"
     CDS             3937..4779
                     /locus_tag="VGE48_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041864.1"
                     /translation="MNEGFGSRSMHRLDRSFETRPAMIEAHAKALSAFEADSIDLEEF
                     RDLYGSEVIDADLAKVARLKSNFAPEDRNKFASEIMEGIIYDQSERSDWLGPYAQTIR
                     ASEYDDYVNGIDLVVEFDEPERSRSHLALGVDATFSRTNIEKKLRRVKDEIDSGSLAS
                     IKYFRSQNGRFMGRLTNVPRVVTGMEKDRLLELATIWNQDDNKTLAAHPAQRLLLAQI
                     AQQLKVFKGYAERTGKHQFVRPFDETLATIRGVSEKKTSIDVSSVRDDTVHKEIVSCL
                     EMFK"
     gene            complement(4783..5478)
                     /locus_tag="VGE48_00365"
     CDS             complement(4783..5478)
                     /locus_tag="VGE48_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY1041865.1"
                     /translation="MNIQNIRRDYLLPGAIVLAGLLLALTVYAVRTDKSPKIGAGDPD
                     MVKPVTPADRIIGNPKAPVTIIEYADIDSMYSKQFQATMQQLMSEYAAGGQVAWVYRH
                     FPLLDQHAGAASHAEAAECAGFLGDDDAFWRFIDALQSAAPGESEFPVAGYESIARGL
                     GLPEAEFMQCLTNGRFTKKVYEDTQNALDAGANGAPYTIVMVEGQEPVAIQGALTYED
                     MKRVIDESIAKVR"
     gene            complement(5537..5821)
                     /locus_tag="VGE48_00370"
     CDS             complement(5537..5821)
                     /locus_tag="VGE48_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041866.1"
                     /translation="MRAYLQIIADLSEPEQDSLLVGFLEEFPGLSLHPVPGASVSPGS
                     IICTLTGVRDESLTRSVADFLWQKVSNRNVSRVKVRNELLPERDVLLLAA"
     gene            complement(5890..6468)
                     /locus_tag="VGE48_00375"
     CDS             complement(5890..6468)
                     /locus_tag="VGE48_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY1041867.1"
                     /translation="MREITFDIETANVLPTMARQDVTRLDLAVVAIHDSETGEFSSYS
                     QEELPKLWPIIEQADTLIGFNSDSFDIPLLNKYYPGDLTKIRSIDLLVEVQKALGRRI
                     RLQSLAEATLKVGKSGDGLQSVRWWQEGLYDKVREYCIMDVKITRQLYDYALKNGALK
                     FKDLNQARDLKMDTSKWQEPPEAAAFTHTLGL"
     gene            complement(6470..7756)
                     /gene="hisS"
                     /locus_tag="VGE48_00380"
     CDS             complement(6470..7756)
                     /gene="hisS"
                     /locus_tag="VGE48_00380"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY1041868.1"
                     /translation="MTDRKPLSTESYKGVHDYYPEDWAKVQAAFDTIRGTLRSYGFEE
                     YQASPLERAEIYEAKTSEEIVSQQTYTFEDRGGRRVTLRPEMTPTLARMIAGKRRELV
                     FPARWFSIGNRFRYERPQKGRSREFYQADVDIVGIKGPMAEGEAVVMAYEIFKAFGAK
                     TKDFRIRIGSRTLMNTAGAVLGLSPEQVTEYLSLVDRKDKMMPEAFEEARATYRRNGT
                     DPLELVEARSDMGVDSVYQELLGLIEAFKERGMDNVELDPTIARGFLYYTGLVFEVFD
                     TNPENPRALLGGGRYDGLVALFGGEEIPAVGFAIGIETLMDFLTTHNLLPKVLPGADL
                     FVGTPSEDDIARAQQFADQMRADGLNVLVHTGGRALGDQVKEAVRRGIPYFVAIGEEE
                     ALYKRAKLKELASGTEVEIGTDLIATHIYKVRFDGN"
     gene            complement(8039..8611)
                     /locus_tag="VGE48_00385"
     CDS             complement(8039..8611)
                     /locus_tag="VGE48_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY1041869.1"
                     /translation="MEEFQLEQGERVIRTVRKHWFALLLSLLPYALLAFLPTLLISFL
                     TGMTAANPGTAPLLQSLTPANPWFRLFLGLWWLSLWIGAFTSFTQYYLNHWVITTLRI
                     VRIRQHGFFAREVASFHLIRVQDVTTEVNGIFADLLGYGHVRVQTAGTASKNFVMDGI
                     DDPQGMRDLIMSEIAAQYREKSATFIGMHT"
     gene            complement(8615..8854)
                     /locus_tag="VGE48_00390"
     CDS             complement(8615..8854)
                     /locus_tag="VGE48_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041870.1"
                     /translation="MQEPLNPLPVPAFLAGANIESVISIILLALAAVWLVYTVVSAYH
                     WLRYGHRSAVAIPALITHVAVSWLLASYAGSGLPL"
     gene            complement(8854..9594)
                     /locus_tag="VGE48_00395"
     CDS             complement(8854..9594)
                     /locus_tag="VGE48_00395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEY1041871.1"
                     /translation="MTDQDLLIRDKYAGDRNADLTEDLARLKNGEPLAYVIGWIPFLG
                     LRIGLDSRPLIPRPETEWWTEALCAHLEERFGDRPFSFLDLCAGSGVIGLSVLARFPN
                     AKVTFAELVEAHTEQINRNIEANGLDAARAVALRSDLFEALSQMKFDIIATNPPYIPS
                     ERTLDASVISYEPKEALFASEDGLSVIRRIAEDAPAHISKGGELWMECDVDNIDIAKD
                     LILEHGAKSAEIRTDLYGRPRVAVGYYE"
     gene            9646..10080
                     /locus_tag="VGE48_00400"
     CDS             9646..10080
                     /locus_tag="VGE48_00400"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEY1041872.1"
                     /translation="MHAIWNLFFTVFFVWLLTMSFFWLSDQGLLIADIPAFHLVLLSI
                     ATFRLVRLFTYDHIMEPIRSWLSGFQERTFLGTGAALLKCPWCTGMWFAFLAYVGYAA
                     IPNVVLPIALILSIAGVASLFQLLANLIGWSAEEKKQRVERM"
     gene            complement(10125..>10472)
                     /locus_tag="VGE48_00405"
     CDS             complement(10125..>10472)
                     /locus_tag="VGE48_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY1041873.1"
                     /translation="VGGIPYRTTEDELRTAFEEAGAVTSASIIMDRMTGRSRGFGFVE
                     MASEAEAQAAIDRWDGKELDGRMLSVSMARPQGDRPAGDRGGRGFGGGDRGGFGGGRG
                     FGGGSNGGYQGGY"
BASE COUNT         2254 a         3068 c         2997 g         2153 t
ORIGIN      
        1 ttcgactgga acaagggctt caagttctcg acctacgcta cctggtggat ccgccaggct
       61 atcacgcgcg cgctcgcaga ccagtcccgc accattcgta tcccggtgca catggttgag
      121 accatcgcca agtacaagca ggtatcgcgc cgcctcgcac aggtgcttgg tcgcgacccg
      181 cagccggagg agatcgctat cgagatggcg gtcgaggttg agaagatcta ccagatcgag
      241 aagatcaacc aggacacgct ttcacttgag aatccgatcg gttccgatga tgacgatcgc
      301 tctaccctcg gtgacttcat cgcggacgat aaaatcccgt ctcctatcca ggagtcttct
      361 gagcgtatcc tcggagagca ggtgcgcgat attcttgagg acctttctcc taaggagcgc
      421 aagatccttg agatgcgcca cgggcttctc gatggcgtgt atcacacgct tgaggaggtg
      481 ggtaaggagt tcggcgttac gcgcgaacgt atccgtcaga tcgaagcgaa ggcgcttgag
      541 aagatcagaa cgcacgagaa gtcacgaagg cttaagagtt actaaacaaa agacccgtcc
      601 tacgcgggtc ttttgcatta taagcacatc actatgctta aaggccggcg attactcgcc
      661 ggccctgctc acacatcatg aattgcgagg ctttatgccc gccgcgcggc acgcgtgcac
      721 gaaatcgcgc ttcatgcccc agtgtgtggc acgaagcttc ttgtcgactt ccttcgcccg
      781 aaagacaatc actttttgca cctcgatcgg tgtttcgggc tgagcatcga tgcagccgat
      841 gatctcgttc cgtccagggt caaagagaat gcgttgaccc tcgtcgagac gaaagtagac
      901 atcgacaggg aagttcggcg atctcgcaaa gccgaagttc cgttccgggt cgattgagac
      961 aatgacccct tcgtactgcg tgtcctccac gatacttcct ccgctgtgtt gcactatctg
     1021 cagtgaaaat agcacattca ttaagaatgt acatgaatcc ttcgtatcgt agaatgaaga
     1081 catgaccgac cagaccgcct tcttagaagc ctatacccgt ctcaacgagg cccagaagaa
     1141 ggcggtagat accgtagagg gtcctgtgct cgtggtggca ggtccgggaa ccggcaagac
     1201 ccatatcctc acacttcgga tcgcgaatat tctccggacc acgactgaca cctcacccga
     1261 gaacattctc gtcctcacgt tcaccgattc cgcagcacgc accgtctcaa gacgtcttgg
     1321 aacctttatc ggcgacgaag ccgcgcgcaa ggtgaacgtc tttaccttcc acggattcgc
     1381 tggggaaata cttcgaaggc acccggaagc cttccctgag ttcgcggacc aacggctcat
     1441 gggggaagtg gagcagatac tcgtgtggcg cgcggtgctc gagaacgcaa ccgactcgaa
     1501 gctccgcacc ccgaagtccc cattccatta cctaaaggat ctcaagtctc tcgaggactc
     1561 gctcacgcgc gagtgcgtat caactgacga ctaccgcgcc tggctcgacc gagaagagca
     1621 gtccttgaag gacgacccga agctcgtgta tacgaaagga gatcgcgcag gggacctgaa
     1681 cgcagccgga cagaagcgcg cggagcgcct cattaaaggt cgtgaagccg cagaccttat
     1741 cgatgcgtac cgcacgctga aagaggagct cggcgtgtac ggatacaccg acatcctccg
     1801 gatcgtgacg gatgcgctca aggaagacga ggccttgcgc gcagagcttc aggagcgcta
     1861 ccagtacgtg ctcgcggacg aacaccagga tgcgaacgca ctgcagcatg ccttgctaga
     1921 cgcgctcgcg ttcgacgagc acccgaacct cttcattgtg ggagatgaga agcaggccgt
     1981 gtacggcttc cagggagcgg acaccacgca ctttgagacg ttcacgagcc tgtacccgag
     2041 gacaaccgtt atcccgctca cggagaacta ccgttccttc caggacattc tcgatctctc
     2101 gcacggactg ctcgctgatc tcccgtcctc tactggaggc catgtagagc tttctggctt
     2161 ccgcgggaag ggtggaaccg cccatatcct tgccgcaacc gacgcgctcg cagaacgcga
     2221 ccagatagcc actctagtcc aagaggcgat tagtgagggt gtcccgcccc atgagatcgc
     2281 cataatcacg ctgaagaacc gcaccgcgga cctcttcgcg ctccatctgc gtgcccgcgg
     2341 tatcgcggcg cttcgtgcgg gggatataga cctcgaaggc aggcctgcga ttcggttcct
     2401 cctctcctta atggatgcgg cagccgaccc taacgcgcct catgcgctgc gtgaggcgct
     2461 gcttgcgccg tggtggcagg cgcccttgga agagcgtgcc cggttcctgc gcacgcatgg
     2521 ggattgggaa atcgaggaat cattgaagga agcgttccca gagatcgccg tcatcgtagc
     2581 ggggctgcgc gagaaggtac tcgtggtgcc gcctttgaac gcattctcat acatcctctc
     2641 agactcaggc gcccgctcgt acttcctctc ctcaggagag accgtgcacg aggacctgcc
     2701 tttggtacgt cagctcatga cccatatcga ggagctcgca acccgtgatc cgaatgcttc
     2761 cttcaaggaa gttatggaaa gcttcgggaa agcgcgtgag catgaggttg gagccataaa
     2821 gacctctcgc acccagcaag agggactggt cacggttata accgcgcata aagcgaaggg
     2881 catggagttc gagcgggtgt ttgtaaccgc cttgtccgcg cgtgaatggg aagggcgcgg
     2941 caggtcctct cttgtaccgt ccccgttctc agcgagtcgc gagcgtgaag aacttatacg
     3001 cctcttctac gtagcgctca cacgcgccaa ggaccggctt gtcctctcgt tcccccgcga
     3061 gagcgcggac gggaaggagg aagcgcccat gtccctgttg ccgcaaggac ttccggagat
     3121 ccgggtatca aaggacccgc ttccgaagct ccatgcggtt gtggacgctc caaagctcgt
     3181 ctgcgaactc actctcgcgt acctcacgca cgaaggcctc tcgccatccg cattcaacga
     3241 atacctcgag tctccggcga ccttcttcgc taagcgcgtc ctgcgcctcc gagagccgga
     3301 gaaccgcagc atcgtgcttg gcaacgctgt acatgccgct atcgccgcgt acctgcgcgc
     3361 gaaggatgcg tcggaagaag agcgtgaagc ggcggcatac gcggaactca cgcgttcctt
     3421 caggaaatcc ctcctcccgc gcggagacac cttcgcagcg ctcgagcgcc aggcacgcac
     3481 ctaccttacg gcataccttt caagcgatct cttagggcgc gaggctgttg ccgtagagga
     3541 gacgttccgt ctcgagcgaa cggtcggcga gcagaacata cttctcaagg gtaaagtaga
     3601 tgctgtattc aagggagata cgggtgagtg catcgtagac ttcaagacct cctccaccat
     3661 agacaagaag gatcaggaga agtttgaacg ccagatcgcg ttttacgatc ttctcctgcg
     3721 caataacggg caccagactg aggacggcct catcatccag gtggggggcg agataacgga
     3781 gtatcctgta gcgcttaatg acgagacccg cgcgtccttt gaggcgtgtc tcaaggacgt
     3841 gctcgaagag ctcatttcgg ggaaatggcg ggaaggggag ccaagtccgt acgacgatct
     3901 actcgggctc ttaggcgaat aagctatact tttcctatga atgaaggatt tggttctcgc
     3961 tcgatgcaca gattggaccg ctcgttcgag acacgacccg caatgataga ggcgcacgcg
     4021 aaggcactca gtgcattcga ggcagattct attgatcttg aggaattcag ggacctgtac
     4081 ggaagcgagg taatcgatgc ggatcttgcg aaagtcgctc gacttaaatc gaactttgcc
     4141 cctgaggatc gcaacaaatt cgcatcagaa atcatggagg gaatcatcta tgatcagtca
     4201 gaacgttcgg attggcttgg tccatatgca cagaccatta gggcgagtga gtatgacgat
     4261 tatgtgaacg gtatagacct tgtggtcgaa ttcgatgagc ctgaacgttc gcgtagtcac
     4321 cttgcccttg gtgtagacgc gactttcagt agaacgaata tcgagaagaa gctgcgacgc
     4381 gttaaggatg agatcgattc tggctcgctt gcgagcataa agtacttccg ttcgcaaaac
     4441 ggcaggttca tgggaagact cacaaacgtc ccacgcgtgg tgacaggtat ggagaaggat
     4501 cgactgcttg aactcgccac catttggaat caggatgaca acaagaccct tgcggcacat
     4561 ccggcacaac gcctcctgct cgcgcagata gcgcaacagc tcaaggtctt caaaggatac
     4621 gcagagcgaa ccggcaagca tcagttcgta agacccttcg acgagacgct cgcgaccata
     4681 cgcggtgttt cagagaaaaa gacgtctatc gacgtctctt ccgtgcggga cgatacggtt
     4741 cacaaggaga tcgtatcttg tcttgagatg ttcaagtagg aattaccgca ccttcgcgat
     4801 cgactcgtcg attacgcgct tcatgtcttc gtaggtgagt gctccctgga tagcaaccgg
     4861 ctcctggccc tcgaccatca cgatcgtgta cggcgctcca ttagccccag catcaagcgc
     4921 attctgtgtg tcttcgtaca ccttctttgt gaagcggccg ttcgtgaggc actgcataaa
     4981 ttcggcttct ggaagcccaa gtcctcgtgc gatcgattca tagcccgcaa caggaaactc
     5041 gctctctcca ggagcagcgc tctggagcgc gtcaatgaag cgccagaacg cgtcatcgtc
     5101 cccgaggaat cctgcgcact cggcagcctc cgcgtgtgat gcggcgcccg catgctggtc
     5161 taggagcgga aagtggcggt acacccacgc gacctggccg ccggcagcgt actcgctcat
     5221 gagctgttgc attgtggcct ggaactgctt gctgtacatg gaatcgatat ccgcgtactc
     5281 gattatagtg acaggtgcct ttgggttgcc gatgatgcga tctgccggag taacgggctt
     5341 aaccatatct ggatctccag ccccgatctt tgggcttttg tcggtgcgta ccgcatatac
     5401 ggtgagggca agcagtaatc ccgcaagcac gatggcgccc gggagcagat agtcgcgacg
     5461 aatattctgg atgttcatga aaaaagcata gcatgcaaac atgaacaacc ccgcgcgagc
     5521 gcggggttgt gccagttcag gcggcgagga gcaatacgtc cctttcgggc aacagctcgt
     5581 tgcggacctt cacgcggctg acattcctgt tggaaacctt ctgccacagg aaatctgcga
     5641 cgctccttgt caggctctca tcgcgcacgc cggtgagcgt gcagatgatc gagcctgggg
     5701 aaaccgaggc tccagggacc ggatgcaagc tgagccccgg aaattcctcg aggaagccga
     5761 cgagcaggct gtcttgctcg ggttcgctga ggtcagcgat gatctgaaga taggcgcgca
     5821 tgtaaccccc tatggatagc gcaaatggga cacctgcgat gaaaatagca cgaaaacgat
     5881 ataaatcaac tacagcccca gggtatgcgt aaacgcggct gcctcaggcg gctcctgcca
     5941 cttactcgta tccatcttaa ggtcccgggc ctggttgaga tctttgaatt taagggcgcc
     6001 gttcttaagc gcgtaatcat acagctggcg cgtgatcttc acgtccatga tgcagtactc
     6061 gcgtaccttg tcgtagagtc cttcctgcca ccagcgcact gactgaagac cgtctccaga
     6121 cttaccaacc ttgagggttg cttccgcaag ggactgaagc cgtatccttc tcccgagcgc
     6181 cttctgcacc tcgaccagga ggtcgataga acggatcttc gtgaggtcgc ccggatagta
     6241 cttattcaga agcgggatat cgaaggaatc cgagttgaac ccgatcaggg tatcggcttg
     6301 ctcgatgata ggccagagct tcgggagctc ttcctgggaa taggaagaga attcgccggt
     6361 ctccgagtca tgtatggcaa cgaccgcaag gtcaagacgc gtcacgtcct ggcgcgccat
     6421 ggtagggagg acgttggccg tctcgatatc gaacgtgatc tcgcgcatgc tagttgccgt
     6481 cgaaccgaac cttgtagata tgggtggcga tgagatcagt gccgatttcg acctccgtcc
     6541 cgcttgcaag ctccttcagc ttcgcgcgct tatagagcgc ctcttcctcg ccaatcgcga
     6601 cgaagtacgg aatgccgcgg cgcaccgctt ccttcacctg atccccaagc gcgcggcctc
     6661 cggtgtgcac gagcacattc aatccgtccg cacgcatctg atccgcgaac tgctgcgccc
     6721 gcgcgatgtc atcctccgat ggtgtaccta caaacaagtc cgcacccgga agcaccttcg
     6781 gaaggagatt gtgtgttgta agaaaatcca tcagggtctc gatgcctata gcgaacccta
     6841 cggccgggat ctcttcgccg ccgaagagtg ccacgagccc gtcgtagcgg cctccgccca
     6901 gaagcgcgcg cggattctcc gggtttgtgt cgaacacctc gaacacgaga ccggtgtaat
     6961 agaggaaacc tcgtgcgatc gtaggatcta gttcgacgtt atccatgccg cgctccttga
     7021 acgcctcgat aaggccgagg agctcctggt acacggaatc gacgcccatg tctgaacgag
     7081 cttcgacaag ttcgagcgga tcggtgccgt tcctgcggta cgtagcgcgc gcctcctcga
     7141 acgcttccgg catcatcttg tccttgcggt ccacgaggga gagatattcc gtaacctgtt
     7201 ctggagagag tccgagcacg gcgcctgccg tattcatgag cgtacgggag ccgatacgga
     7261 tcctaaagtc cttcgtcttc gcaccaaacg ccttaaagat ctcgtacgcc ataactacgg
     7321 cctcgccttc ggccatcggg cccttgatgc ccacgatatc gacatcggcc tggtagaact
     7381 cacgggaacg acccttctgc gggcgctcgt accggaagcg gttgccgatg gagaaccagc
     7441 gggcagggaa cacgagctcg cggcgcttcc cggcaatcat acgcgcgagc gttggcgtca
     7501 tctcagggcg cagcgtcacg cggcgcccgc cacgatcctc gaaggtatag gtctgctgcg
     7561 agacgatctc ctcgctggtc ttcgcctcgt agatctctgc gcgctccaag ggagatgcct
     7621 ggtattcctc aaagccatac gagcggagcg tgccgcggat ggtatcgaac gcggcttgga
     7681 ccttcgccca gtcctcgggg tagtagtcat gcacgccctt ataggattcg gtggaaaggg
     7741 gcttacggtc ggtcatgcat cgattctagc aaacgaaacg ccccgccgac acagtggtcg
     7801 acgggacgcg ggtgggtttc aggcgcaggc tgcgcctccg agcatctgat tcagtctcag
     7861 ttcttgagac ggattcctgt tggcacgcgc aaccgcgtga caatcccggg tcaggggacc
     7921 cttcgggtcc agaggaaacg gacgttgccg actgctcaag gcggccggct tctggtgcga
     7981 acctatcact ttctttttct cccttgctgg ttccgttggt gattatagca cggcatcgct
     8041 aggtatgcat gccgatgaag gtggcgctct tctcgcggta ctgcgccgcg atctcgctca
     8101 tgatgaggtc gcgcatgccc tgcggatcgt caatgccgtc catgacgaag ttcttggacg
     8161 cggtgcccgc ggtctgcact cgcacgtgtc cgtacccgag taagtcggcg aaaattccgt
     8221 tcacctcagt ggtgacgtcc tgtacacgga tcagatggaa gctcgctact tcgcgtgcga
     8281 agaagccgtg ctgcctgatg cgcacgatgc gcagggtcgt gatgacccag tggttgaggt
     8341 agtactgcgt gaacgaggtg aacgcgccta tccacaacga cagccaccac aatccgagga
     8401 agagacgaaa ccacgggttc gcaggcgtga ggctctgaag caacggcgcg gtacccggat
     8461 ttgccgcggt cattcctgta aggaaggaga tgagtagggt tggtaggaag gcgagaagcg
     8521 cgtacgggag tagggacaat agaagcgcga accagtgctt cctcacagtc cgtatcacgc
     8581 gctcaccctg ttctagttga aattcttcca tagactatag aggaaggccg cttccggcat
     8641 agcttgcaag tagccatgac accgctacgt gcgtgataag cgcggggatc gcgaccgcag
     8701 agcgatgccc gtagcgcagc cagtggtacg cggacacgac cgtataaacg agccataccg
     8761 ccgcaagcgc gagcaggatg atcgatatga cgctttctat attggcgccc gcaaggaacg
     8821 cgggtacggg aagcggattc aggggctcct gcatcactcg tagtatccca cggccacacg
     8881 ggggcggcca tacaagtccg tgcgtatctc agcgctcttc gcgccgtgct caagaatcaa
     8941 gtccttcgcg atatcgatgt tatcgacatc gcattccatc cagagttcac cacccttact
     9001 tatatgagcc ggtgcgtctt ctgcgatgcg acggataacc gaaaggccgt cttcgctcgc
     9061 gaagagcgct tccttgggtt cgtacgaaat gacgctcgca tcaagcgttc tctcggaagg
     9121 gatgtatggg ggattggtag cgatgatgtc gaacttcatt tgtgaaagcg cttcgaacag
     9181 atcgcttcga agcgcgactg cacgagccgc atcgaggccg ttcgcctcga tgttcctatt
     9241 gatctgttct gtgtgcgctt ccacgagctc cgcgaacgta accttcgcat tcgggaatcg
     9301 cgcgagcacg gaaagcccta tgacacccga acccgcgcac aggtcgagga aggagaatgg
     9361 acggtccccg aaacgttcct caagatgcgc acagagcgcc tccgtccacc attccgtctc
     9421 agggcgggga atcaagggac gcgagtcgag cccaatgcga agacctaaga agggaatcca
     9481 gccgataaca taggcgagcg gctcgccgtt cttgaggcgc gcgaggtctt ccgtgagatc
     9541 tgcgtttcgg tctccggcgt acttgtcgcg gataaggagg tcctggtcgg tcatataccc
     9601 actctaccgg attcgatacc tacgggaagg gtatagtgtc tccatatgca tgcaatctgg
     9661 aaccttttct tcacggtctt cttcgtctgg ctcctcacga tgtccttctt ctggctttcc
     9721 gatcaagggc tcctcatagc agatattccg gccttccatc tcgtgctcct ctcgatcgcc
     9781 accttccgtc tggtgcgtct cttcacctac gaccacatca tggagcctat tcgctcctgg
     9841 ctctccggat tccaggaacg cacgttcctc ggcacgggtg ccgcacttct taagtgcccg
     9901 tggtgcacgg gtatgtggtt cgcgttcctc gcgtacgtag ggtatgcggc gatcccgaac
     9961 gttgtgcttc caatcgcgct catcctctcg atcgcaggtg tcgcttcctt gttccagctc
    10021 ctcgcgaacc tcatcgggtg gagcgcggaa gagaagaagc aacgggtaga gcgcatgtaa
    10081 aaggaaaagg cccctcaagc gaggggcctt ttccgttagg gagactagta gccgccctgg
    10141 tagccaccgt tggagccgcc gccgaagccg cgaccaccac cgaagccacc acggtcgccg
    10201 ccgccgaagc cgcgtccacc gcgatcaccc gctgggcgat caccctgagg acgtgccatg
    10261 gagaccgaga gcatgcgccc gtcgagctcc ttgccgtccc agcggtcgat agcagcctgg
    10321 gcctcagcct cagatgccat ctccacgaag ccgaagccac gggaacgacc ggtcatgcgg
    10381 tccatgataa tgcttgcgga cgtcactgcg cctgcctcct cgaaggcggt gcggagctca
    10441 tcctcggtcg tgcggtacgg gatacctccg ac
//
LOCUS       DASWXU010000014         4313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_830024, whole genome shotgun sequence.
ACCESSION   DASWXU010000014 DASWXU010000000
VERSION     DASWXU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830024"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGE48_00410"
     CDS             complement(<1..198)
                     /locus_tag="VGE48_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1041874.1"
                     /translation="MKTYEGACHCRNVRYEVELDLSQPAMECNCSHCQIKGFILQFTS
                     PDKFKILQGEEELKAYRFNKHV"
     gene            complement(208..1428)
                     /locus_tag="VGE48_00415"
     CDS             complement(208..1428)
                     /locus_tag="VGE48_00415"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006072036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY1041875.1"
                     /translation="MAAFAYFKHNVGKAADLLNLTDEERASFMNPDRVLRATLSVAMD
                     DGNTKEFPAYRVQFDNSRGPYKGGIRFHPDADEDEVSALAAMMAIKCAVVDIPFGGAK
                     GGVTVDPKHLSEGEIDRLSRAYVQAFSEHLGPDTDVPAPDVYTNPHIMAIMLDEYEKI
                     IGASAPAMITGKPLENGGVVGRDTATADGALYVLEAVLEDQHRSLEGVEVSIQGAGNA
                     GAQAARLVVKDGGKVVALADSRGTLVNEEGIDVDAVLALKEDGKSVHDATAYGSPGNA
                     EDVIAAACDVLVPAALEDAVTEKNAGSVMATVVLEIANGPVTPEADDLLTARGIGVIP
                     DVLANAGGVTVSYFEWLQNRSGETWDRARVTERLKATMRDAYRDVADFAHERGITMRE
                     AAYALALTRILEAR"
     gene            complement(1439..1981)
                     /locus_tag="VGE48_00420"
     CDS             complement(1439..1981)
                     /locus_tag="VGE48_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY1041876.1"
                     /translation="MTEDQLIGKKVPDVTFKTRVRDESVGGENPFRWQDVTTTELFAG
                     KKVVLFGLPGAFTPTCSSTHAPGYEAKYEEFQAAGVDDVICLSVNDAFTMYQWGQKLG
                     LSKVRMLPDGNGEFTQKLGMLVKKENLGFGNRSWRYSLLVEDGEIKKAFIEPGFSDNC
                     EEDPFEVSDAETMLRYLQSA"
     gene            complement(2061..4283)
                     /locus_tag="VGE48_00425"
     CDS             complement(2061..4283)
                     /locus_tag="VGE48_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1041877.1"
                     /translation="MLKRLEIAGFKSFAKKTTLDFSSSVTSVVGPNGSGKSNVAEAFR
                     FALGEQSMKSMRGKRSEDLIWSGSNMAPRANRAAVTIYFDNTKRIFPKLEFDEVSVER
                     AVFRDGASEYAINGSKVRLRDVQELLASANIGETGHHIISQGEADRILTAQPRERRSM
                     LEDALGLRVFEFKKQEAERKLERTEENVAQVEALRREIAPHIRFLAKQVERLDRAEEL
                     AKELRAASQTYFAIEEAYLREERRETEGKLSVVKEKLAALEAELAVFEERVTTDAEGM
                     RRGEAVRKAERELDRLTSERSELAREIGRIEGALNAAKQRSAMVARDPYVKVPREDLK
                     ALREEVERQADAVENAEPGAIHAALKTLRAAVFAFFERYGTGNDDILLEEEQAAHDLE
                     AKRAELSVRDNALEDAVSQARSALTKAREASMAYEETGREQNRRLLELADEKATAESE
                     RARLSARMGELDYLSEEMEQGRREIVALVGVNALSYEALERLPEEDRRVQEDRKRSME
                     RLKIRVEEAGGGGEDVRIEYKEATEREAFLASELEDLAKSAAGLKELIADLDAELQKS
                     FADGLQKVNASFGEFFSLMFGGGGARLVLEELSQPGSEDEDEEAFEEEAPKRGKYQKP
                     GIEISVNLPKKRVQALMQLSGGERALTSIALIFAMSQVNPPPFLILDETDAALDEANS
                     RRYGDMIESLSKKSQLIVITHNRETMSRAGILYGVTMGADGISKLLSVKFEEAMAVAK
                     "
BASE COUNT          799 a         1389 c         1149 g          976 t
ORIGIN      
        1 cacatgcttg ttaaagcggt acgccttgag ctcctcctcg ccctggagga tcttgaactt
       61 gtccggactc gtaaactgaa ggatgaatcc cttgatttgg cagtgtgagc agttgcactc
      121 catcgcgggc tgcgagaggt cgagctcgac ttcgtagcgt acgttccggc aatggcaggc
      181 tccttcgtat gttttcatgg gactatccta gcgcgcttca agaatacgtg taagcgcgag
      241 cgcataggct gcttcgcgca tagtgatgcc acgctcgtgc gcaaaatctg cgacgtctcg
      301 gtacgcgtca cgcatcgtgg ccttgagacg ctccgtgacg cgagcacggt cccaggtctc
      361 gcccgagcgg ttctgcagcc attcgaagta cgagactgtc acgccgcccg cgttcgcgag
      421 cacgtccggg atcacgccga tgccgcgggc cgtaaggagg tcatccgctt ccggcgtaac
      481 cggaccgttc gctatttcaa gcaccacagt cgccatgacc gagcctgcat tcttttcagt
      541 aaccgcgtcc tcaagtgccg ccggcacgag cacgtcgcac gctgcggcta tcacgtcttc
      601 cgcgttccca ggcgagccgt atgcagtcgc gtcatgcact gacttcccgt cttctttcag
      661 cgcgagcacc gcgtcgacat caatgccctc ttcgttcacc agagtgccgc ggctgtccgc
      721 gagcgcgacg accttacctc cgtccttaac cacgagccgc gcagcctggg cacctgcatt
      781 ccctgcaccc tggatcgata cctcgacccc ttcgagactc cggtgctgat cctcaagcac
      841 cgcttcaagc acatacaggg cgccgtctgc ggttgctgta tcacgcccaa caacaccgcc
      901 attctcaagc ggctttcccg tgatcatggc aggggctgat gcgccaatta tcttctcgta
      961 ctcgtcgagc atgatggcca tgatatgtgg gttcgtatat acgtcgggcg ccggcacgtc
     1021 cgtgtcagga cccaagtgct ccgagaatgc ctgaacatat gcacgcgata gcctatcgat
     1081 ttctccttct gagagatgtt tcgggtctac ggttacgcca cccttcgctc ctccgaatgg
     1141 aatgtccacg actgcacact taatagccat catcgctgcg agcgcagata cttcgtcttc
     1201 atccgcgtcg ggatggaaac ggattccgcc cttgtacgga ccgcgagaat tgtcgaactg
     1261 cacgcggtat gccgggaatt ccttggtgtt cccatcatcc atcgcgaccg acaaggtagc
     1321 gcgtagcacc ctgtccggat tcataaacga ggcacgttcc tcgtccgtga gattgaggag
     1381 atctgctgcc ttcccgacgt tgtgcttgaa gtatgcgaac gcagccatgg caaggatctt
     1441 aggcgctctg gagatacctg agcatggtct cggcgtccga cacctcaaac ggatcctcct
     1501 cacagttgtc ggagaagcct ggctcgatga atgccttctt aatttcgcca tcctccacaa
     1561 ggagcgagta gcgccatgaa cgattgccga agccgaggtt ctctttcttc acgagcatgc
     1621 cgagcttctg ggtaaactcg ccgttgccat ctggaagcat acgcaccttg gagaggccga
     1681 gcttctggcc ccactgatac atcgtgaacg catcgttgac ggacaagcag atcacatcgt
     1741 ccacgccggc agcctggaac tcctcgtact tcgcttcgta gcccggagca tgcgtcgagg
     1801 agcaggtcgg cgtgaatgcg cccggaagac caaatagcac taccttctta cctgcaaaaa
     1861 gctccgtggt ggtcacgtct tgccagcgga acgggttctc tccgcccacg gactcgtcac
     1921 ggacacgggt cttgaacgtt acgtctggta cctttttccc gatcaattgg tcttcagtca
     1981 tacgtaatag ctagttagta caaaaagcat acgccttcct caggaggcgt ccatggggat
     2041 aagaatatcg atctgagagg ctatttcgct accgccatcg cctcctcgaa cttcacggaa
     2101 aggagcttcg agataccgtc tgcacccata gtgacgccgt agagtatgcc cgcgcggctc
     2161 atggtctcgc ggttgtgcgt aataacgatg agctgggact tcttcgacag gctctcgatc
     2221 atgtcgccgt agcggcggct gttcgcctcg tcgagcgccg cgtcggtctc gtcgagaatc
     2281 aaaaatggcg gcggattcac ctggctcata gcaaagatga gcgcgatgga agtaagcgcg
     2341 cgctcaccgc ctgagagctg catgagtgcc tgtacgcgct tcttcgggag gttcaccgat
     2401 atctcgattc ctggcttctg atacttgccg cgctttggtg cttcttcctc gaacgcttcc
     2461 tcatcttcat cttcagatcc tggctgagac aattcttcga gtaccaaacg cgcgccgccc
     2521 ccgccgaaca tgagcgagaa gaactcgccg aaggacgcgt tcaccttctg caaaccgtcc
     2581 gcgaacgact tctgtagctc ggcatcgagg tccgcgatga gttccttgag ccctgccgcg
     2641 gacttcgcga ggtcttcgag ctcagacgcg aggaacgcct cgcgctcggt agcctccttg
     2701 tactcgatac gcacgtcctc gccgcctccc cctgcttcct cgacacgaat cttgagccgc
     2761 tccatggagc gcttccggtc ttcctgcacg cgccgatctt cttcaggcag tctctcaagc
     2821 gcttcgtatg aaagggcgtt gacgccaacc aaagcgacaa tctcgcgtct gccctgctcc
     2881 atctcttcag aaagataatc aagctctccc atgcgcgcgg acagtctcgc acgctcgctc
     2941 tcggcagtag ccttctcatc cgcgagctca aggagtctgc ggttctgttc gcgtccggtc
     3001 tcctcgtatg ccatggacgc ttcgcgtgcc tttgtgagcg cgcttcgtgc ctgagacacc
     3061 gcgtcctcga gcgcattgtc ccgcacggaa agctccgcgc gcttcgcttc caaatcatgc
     3121 gctgcctgtt cttcttcaag gagaatgtcg tcgttaccgg tgccgtaccg ctcgaagaat
     3181 gcgaataccg cagcacgaag cgtcttgagc gccgcatgga tcgctcccgg ttctgcgttc
     3241 tcaactgcgt cggcctggcg ctctacctcc tcgcgaagcg ccttcaagtc ctcgcgcggc
     3301 accttcacat acggatcgcg cgctaccatg gcactccttt gcttcgccgc gttcaaggcg
     3361 ccctcgatgc gtcctatctc gcgtgcgagt tccgaacgct cgctcgtcag acggtcgagc
     3421 tcgcgctccg ccttccgtac ggcttctccg cgacgcatgc cctcggcgtc cgtggttacg
     3481 cgctcctcga acacggcgag ctccgcttca agcgccgcca gcttttcctt aactacgctg
     3541 agcttccctt cagtctcccg gcgttcttcc cgaaggtacg cttcctcgat cgcgaaatat
     3601 gtctggctcg ctgcgcgcaa ttcctttgcg agctcctctg cacgatcaag gcgctctacc
     3661 tgcttggcta gaaatctaat atgcggcgca atctctcttc gaagcgcttc aacctgcgcg
     3721 acgttctctt cggtgcgctc gagctttctt tccgcttcct gcttcttgaa ctcgaatacg
     3781 cggagaccga gcgcatcctc gagcatcgag cggcgctcgc gcggctgcgc cgtgaggatg
     3841 cggtccgctt cgccttgaga aatgatatgg tgcccggtct cgccgatgtt tgcagaggcc
     3901 aagagctctt gcacgtcgcg aaggcgcacc ttcgagccgt tgatcgcata ctcgctcgcc
     3961 ccgtcgcgga acaccgcgcg ctccacagac acctcgtcga actcgagctt cgggaagatg
     4021 cgcttcgtat tgtcgaagta gatggttacg gcggcgcggt tcgcgcgcgg cgccatgttg
     4081 gaaccggacc agatgaggtc ttcgcttcgc tttccgcgca tcgatttcat ggactgctca
     4141 ccgagcgcga aacggaacgc ttccgcgacg ttactctttc ccgagccgtt cggacccacg
     4201 acgctcgtga cgctcgacga gaagtcgagc gtagtcttct tcgcaaagga cttgaatccc
     4261 gctatctcaa gacgcttcag catttcaaaa acagtatagc gtatggaaaa gcc
//
LOCUS       DASWXU010000015         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_222494, whole genome shotgun sequence.
ACCESSION   DASWXU010000015 DASWXU010000000
VERSION     DASWXU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222494"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGE48_00430"
     CDS             <1..183
                     /locus_tag="VGE48_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEY1041878.1"
                     /translation="PPKGVDIVREVGKHPKTGKSIVLYKSKAGLFLKKGLRRIYLPES
                     QDAEKLTPEEAAEYLK"
     gene            423..2015
                     /locus_tag="VGE48_00435"
     CDS             423..2015
                     /locus_tag="VGE48_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY1041879.1"
                     /translation="MTTVKEILAQTSGFSPEAEALVEKAYAFAENAHKDHKRYSGEPY
                     FTHPAAVAKQLAEFGMDASTIAAALLHDSIEDVGVTPEEIDREFGPEVLFLVEGVTKL
                     GKHKYQGGERHAESLRRLLVATASDVRVLIVKLADRHHNMTTLEHVPEEKRRRIALET
                     LEIYAPIADRLGMGALKKELEDLAFPAIDPDAYQHAIEVRKLKNKETEQGLARAQKNL
                     QHALVKKGLTDFSTSVRMKGLWSLHQKLKRKGDDITRIYDIAALRVIVPTLDDCYTAL
                     GVIHALWKPLPGEFKDYISFPKPNGYQSLHTTVMTGEAGIVEMQVRTRDMHQRAQFGI
                     ASHMSYKSLEKSAGKRAFQRLSFPWIRDLVPALLNVKKTTPQNHDAPDVPRWLTDLKE
                     AHAEATETDEFVEGLKSDFFSHRIFVFTPNGDVIDLPTESTPIDFAYAIHSDLGDHMQ
                     GAKANGKLVSFDTKLRNGDVIEVLKSKSAHPTAKWLDYARTSMARRHIRNALDVEARG
                     RPHAESVKPKRSPKERKQKKAK"
     gene            complement(2016..>2898)
                     /locus_tag="VGE48_00440"
     CDS             complement(2016..>2898)
                     /locus_tag="VGE48_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY1041880.1"
                     /translation="LAESIRQYGVLQPLVVTRKEVEKSDGGIQSAYELIAGERRLRAS
                     KLAGLKEVPVVIRAGEQSDKMKLELAIIENLQREDLNAIDRAKALAQLVETFGLSHAE
                     VGAKIGRSREYVSNSIRLLLLPEAIQQAVVGKELSEGHARALLMLNDKPEERDTLFKE
                     IVLKKTSVRMVEQMARRLAQDKIRKHDKTPEMMALEKSLTETLGTRVLIENRPNGGRL
                     LIEFFSPDDLSHIAAMLATREAALLAEAEQPIADPSEPTPAEPVEEAPILPSAAETPQ
                     SPQQPEEDLYSVRNFTV"
BASE COUNT          712 a          797 c          800 g          589 t
ORIGIN      
        1 ccaccgaagg gagtcgatat cgtgcgcgag gtcggtaaac atccaaaaac cgggaagtcg
       61 atcgtgttgt acaagtcaaa ggctggactc ttcttgaaga aaggcctccg tcgtatctat
      121 cttccggaaa gccaggacgc agagaagctt acgcccgaag aggcggcaga atacttgaaa
      181 tagagaacgg ccccgaaatc ggggccgctt ctatacgcat tacgcgcaga gtcgttcagg
      241 agccgcaagt acagcggcgg attgccgagg tttgagctcg agcggatgct ggcagcggtc
      301 ggtattgatt gagcgaccac gcctatgaaa aaccctgcaa aaaggacatg gggacacaaa
      361 aggcagtacc atctacttgc tccctccgtt gcgtgaatct catcaaacag tatccttacg
      421 ctatgaccac cgtcaaggaa atccttgcgc agacaagtgg gttctcgcct gaggcggaag
      481 cgctcgtaga gaaggcgtat gcatttgcag aaaacgcaca caaggatcac aagcggtatt
      541 caggtgaacc ttatttcacc caccccgcgg cagtagccaa gcaactcgct gagtttggca
      601 tggacgcgtc caccattgcc gcggcactcc ttcatgattc tattgaagac gttggcgtaa
      661 ctcctgaaga gatcgatcga gaattcggcc ccgaggtgct ctttttggtc gaaggcgtca
      721 ccaagctcgg aaagcacaag tatcagggcg gagaacgcca cgcagagagt ctgagacgcc
      781 ttttggttgc aaccgcaagt gatgttcgcg tactcatcgt aaaactcgcg gaccgtcacc
      841 acaacatgac gactctggaa cacgtgcctg aagagaagcg tcgtcgcatc gctcttgaga
      901 cgcttgagat ctacgcacct atcgcggatc gtcttggaat gggtgcgctc aagaaagagt
      961 tagaggatct tgcgttccct gccatagacc cagacgcgta tcagcacgcg attgaggtta
     1021 ggaagctcaa gaacaaggaa actgagcaag gccttgcgcg tgcgcaaaaa aatctccagc
     1081 acgcgctggt aaagaaaggg ctcacggact tctcaacgtc cgttcgtatg aaaggactct
     1141 ggagtctcca ccagaaacta aagcgtaagg gcgacgatat cacgcgcatc tatgacatcg
     1201 cggcgcttcg cgtaattgtg cccacgctcg atgactgcta caccgcactc ggcgtaatcc
     1261 atgcgctctg gaagccgctc ccaggagagt tcaaggacta catttcgttc cctaagccga
     1321 acggctacca gtccctccat accaccgtta tgacgggaga ggcaggtatc gtggaaatgc
     1381 aggtacgcac tcgtgacatg catcagcggg ctcagttcgg tatcgcgtcc catatgtcat
     1441 ataagtcgct tgagaaaagt gcgggtaagc gcgccttcca gcgactttcc ttcccgtgga
     1501 tcagggatct cgtgccagcg cttcttaacg ttaagaaaac cactccccag aatcacgacg
     1561 cgccagacgt tccgcgatgg ctcacggacc taaaggaagc gcatgcagaa gcaaccgaga
     1621 cagatgagtt cgtagaagga ctgaagtctg acttcttctc acaccgcatt tttgtattca
     1681 caccaaacgg ggacgttatc gaccttccaa cagagtccac acccatagac tttgcctacg
     1741 cgatacatag cgaccttggc gaccatatgc agggagcgaa agcgaacggc aaactcgtat
     1801 cgtttgatac caagctcagg aatggcgatg ttatcgaagt cttgaaatca aaatcagcac
     1861 atccaaccgc taagtggctt gattacgctc gcacgagcat ggcgcgacgt catatacgaa
     1921 atgcgctcga tgtagaagca cggggtcgcc cgcacgcaga aagcgtaaaa ccgaagcgct
     1981 ccccgaaaga gcgcaaacag aagaaggcga agtagttaga ccgtgaagtt gcggaccgag
     2041 tagaggtcct cttcaggctg ctgaggagat tgcggagtct ctgcagcact cggaagtatc
     2101 ggagcctcct cgaccggctc tgcgggtgtt ggttcagaag ggtccgcgat gggctgctcc
     2161 gcctcagcga gaagcgctgc ttcacgtgtc gcaagcatcg ccgcaatatg cgaaagatcg
     2221 tccggagaga aaaactcgat gaggaggcgt ccgccgttcg ggcggttctc aataaggaca
     2281 cgagtaccga gtgtctcggt aagggacttt tcgagagcca tcatctccgg tgtcttgtcg
     2341 tgcttacgga tcttgtcctg cgcaagacga cgcgccatct gctctaccat acgcaccgag
     2401 gtcttcttga gaacaatttc cttgaacagg gtgtcgcgct cttcaggctt atcgttgagc
     2461 atgaggagcg cacgagcatg gccttctgag agttccttac ccacaaccgc ctgctggata
     2521 gcctccggaa ggaggagaag gcggatggag ttcgatacgt actcgcgtga gcggccgatc
     2581 ttcgcaccca cttccgcatg cgagagcccg aaggtctcga cgagctgcgc gagtgccttg
     2641 gcgcgatcta tcgcgttgag gtcctcgcgc tggaggttct cgatgatagc gagctcgagc
     2701 ttcattttgt cactctgctc gccggcacgg ataactaccg gtacctcctt gagtcctgcg
     2761 agcttggatg cgcgcagacg acgctctcca gcaataagct cgtacgcgga ctggataccg
     2821 ccgtctgact tctctacttc cttcctcgta accacgagtg gctggagcac gccgtactgg
     2881 cgaatagact ccgcaagc
//
LOCUS       DASWXU010000016        11799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_882307, whole genome shotgun sequence.
ACCESSION   DASWXU010000016 DASWXU010000000
VERSION     DASWXU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882307"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VGE48_00445"
     CDS             <1..1452
                     /locus_tag="VGE48_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1041881.1"
                     /translation="RHKCRQSPGALRESVLYERGTFVPRSYYTECMFTPEANKRFGRM
                     RLAPVLLAVLMVLIGAVSAFYVFSQVDRAGREHILERVATISVAVPQEELRELSGTED
                     DLGTPSYETLKLFLTKMRSVNGDARFLYVIGKNAEGNLFFYADSEPAESADYSPPGQV
                     YYEATPAMQALFEDGIRRTEGPDQDRWGLWISGYAPVVDEAGTVVAMLGMDLPASSYL
                     GDAMAYASLPLLISLLLMLGIIATERTRRRETAYLEQKAEFLSIASHEIRTPLTGIRW
                     AIEGLLKRQNPPLNPKTRTVLALVHESCLGLIGRVNNLLDLTALEGGNTARLRPEAID
                     LRDFVEDIADSLALSAQERHVSIDVDGSFAEAGTLTADRQMLHHALFNLLTNAVKYTR
                     EGSVVRIRYARVSGMHQLSVVDQGKGIAAEDQQKIFAGYHRTKEAIRSGAYGTGLGLY
                     LVKKAAELHGGTVTVSSVLGEGSTFTLSLPLNA"
     gene            complement(1449..1826)
                     /locus_tag="VGE48_00450"
     CDS             complement(1449..1826)
                     /locus_tag="VGE48_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1041882.1"
                     /translation="MSTPKKVLIVEDDTLLLVTLTHQFEDAGYTVLSATNGEDGVSLY
                     LSEKPDAVVMDVMMPRKDGVEMLEEIREKAPDHKAPVIVLSNANDMDYVARAMSQGAV
                     AYLLKSDRQIDSVVKLVNERLEH"
     gene            1963..2664
                     /locus_tag="VGE48_00455"
     CDS             1963..2664
                     /locus_tag="VGE48_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041883.1"
                     /translation="MTKFGTLVLLAAFGFGAAGAVHAQGVNLDVGTGIDVSATSSVME
                     ADVMADTETSLSSDAGTSTEAGSATLAEFSIDRNDLTEGTVYSVTRAEDVMTPASLES
                     YVGSLVERDDRVEEIELANGELVIRYKAEAKFLGFIPGSITIRAEVSPEGEVRVSYPW
                     YSFLMAKAESRADLEARLNHDLDVIADDLTEDIEIGSGANVQTVPMNDSRRMALIVER
                     LRSSLYAQGSAEART"
     gene            complement(2708..5803)
                     /locus_tag="VGE48_00460"
     CDS             complement(2708..5803)
                     /locus_tag="VGE48_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1041884.1"
                     /translation="MTKFWTFFLAKHHFTILLAVVLILGGLYAVLAIPKESSPEVQIP
                     MGVVTTVLPGASAEDIETLITNKVEDRVLGIEGVSKVTSSSGDGVSSVTVEFNANADI
                     DKSIQLLKDEVDTVRPELPEEANDPVVSDVNFADQPILIISIAGELAPAELTELGETV
                     ADEIERVRGVSSVSVSGVRERQVQVVVEQAKLRQYGISISDVTNALRASGVAAPAGAI
                     TVDGIRYSVRLEAGLTDTAQVGAIALAGPGGTPIRIADVATVVDGLADPATYSRVSVD
                     GAPSAPALTLSVFKSRGGNILETGSDVKKLLEDLETGPLSGTETVITYDAADDIAHDL
                     AELSKVGLETVVLVMIMLFLTLGWRESLVAAASIPLSFLIAFIGLAASGNTINFISLF
                     SLILAIGILVDSGIVVVEAMHTRLMQLGDKHKAAVAAIKEYAWPLIAGTMTTVAVFVP
                     LFFLSGIVGKFIASIPFTVIFVLLASIFVALGLVPLLALYVIKKEPSALEKRQEEYNE
                     RAKAWYEKYLRSMLGNRQKENKFLWTMAGLFVLSLLLPITGLVKVVFFPAEDMDFVTI
                     AIEKPQGTALTDTDLAVREVEELLYEDPRIESFVSDVGTASFFSSTGGAGSHIANITV
                     NLAEDRKQTSGEIVTDLRKTLASVTSARIVVSEPAGGPPTGAAISVTFVGDDLAALGT
                     AVDQGARVLSEVPGTTNVDTSMKDDGAEFVVRLDSAKAAELGVSPLAVADTLRSALFG
                     TDATDIRAGANDIEVRTKLDLNPGFRDPSETTNATIDAVRSLTVPGSRGPVPLSSIAT
                     IAYEPANAVIRHEDGTRIATLTADAATGGNPIQIASTFTEKMESVELPEGVEMKIGGE
                     SEDVNQSFAEMGIALLAGAALMLAILVLEFNSFRHSLYLLLIIPLSLVGVLAGLFITN
                     SALSFPSMLGIIALAGVIINHAIILMDSIARIGSERTDASLTDVVVEAASTRFRPIIL
                     TTVVTVVGMIPLSYASGIWGPLAFSIMFGLAWSMILTLVLIPILYHRWPGKEVRARFT
                     K"
     gene            complement(5809..7359)
                     /locus_tag="VGE48_00465"
     CDS             complement(5809..7359)
                     /locus_tag="VGE48_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1041885.1"
                     /translation="MNIDALIKRLRTLASLVSVRSKALWRRYRGLKRWQQIVIAVALA
                     ALLIAGISLARSSAKPETLPDGRVVSLATVGELSGTGGSVDLIGSVRSVTEANILAKN
                     GGTVTNVRTRIGASVPAGFVIAELENAAERAAVLQAEGAYDAAVAARSAQSLPDTQES
                     ARDEYRDAYTTLDTIVENDIDAFFGGPTAVGPDLLIYPNANTATRLSRERARIDSLMR
                     SFEANLATANSRSPQALLDEAESIARQVQAFTNELAAAANERDSRATAAQLSALASAR
                     TGVNSVLSSIASARAGLRSGTVSATASADASVKQALGSLRAAQAALERTVVRAPIGGT
                     VNFLPIRVGDYVTPMQHVATVAQNGALEIVAYVSEDNRDLLVAGGEVMIDGAYEGIVT
                     SVAPALDPTTKQIEVRIAVTGETELVSGQSVHIALPDLAPTETAAEPEGPVLLPLAAV
                     KLRAGDRIVFSVGEDSRLVAHQVEVGEVRGDRIEIRTDLPKDLRIVEDARGLSEGEAV
                     RVESETGI"
     gene            7442..8266
                     /locus_tag="VGE48_00470"
     CDS             7442..8266
                     /locus_tag="VGE48_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041886.1"
                     /translation="MKNFHKHVSSHHSFMYLWVVGAMLVLGGLIWSYVASHPTGIIDG
                     QHEGDVRSTVAAFGNQLSAVSLLSPDAADQIRAAYGPYVSEELLASWIENPGTAPGRA
                     TSSPWPDHIEVERVTMNDTDAYDVSGRIILTASTGDAGSVPVFLTVANTEHGYRITAY
                     AEGTNDPVPVVSEGRVTLALGETQTLSGVSITPVRVIEDSRCPMDAMCVWQGTARVEI
                     RLEDGMGQSVMPIELSGEPVTSEIATIWLESVEPYPMASQPTEDASYRFSFRVEAR"
     gene            8271..9929
                     /locus_tag="VGE48_00475"
     CDS             8271..9929
                     /locus_tag="VGE48_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1041887.1"
                     /translation="MTQSEALSILQTGANVFLTGEPGSGKTHTVNAYVAWLRARGIEP
                     SITASTGIAATHVGGMTLHAWSGIGIAERMSEELLDAISSKEHVAKRLQKAKVLIIDE
                     ISMLSGNVLSMVDEVLRTVRHSDRAFGGLQVVLVGDFFQLPPVSRTEVSFAFDAAAWK
                     ALNPVVCYLEEQHRQDDTTFLSVLSAIRAGEWDHTHVSLITAREAEYDELAEGVPQLF
                     THNADVDRINQTELAKLPGSPHTFVMESAGGKVLIESLKRGCLSPESLVLKEGAVVMA
                     TKNNPVAGYANGTLGTVIGFERGTGYPIIETRDGREIAIAPQDWAVEEGGKVRAKISQ
                     VPLRLAWAITVHKSQGQSLDAAAMDLSRAFEYGQGYVALSRVRTLSGVHLLGWSENAL
                     AIHPRVAAFDKELKDASEAAQRAFAALEASGERIPLERNFITAMGGTLEAAPVGERPE
                     KRTTYDETLALLEEGRSLMDIAKVRKLTLGTIADHVGKLIEAGRLDTESLEEQLPGRL
                     RGALPKIHEAFDALGAEKLTPVHSFLKGKYSFDELKLARALYRG"
     gene            9951..10967
                     /gene="mltG"
                     /locus_tag="VGE48_00480"
     CDS             9951..10967
                     /gene="mltG"
                     /locus_tag="VGE48_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY1041888.1"
                     /translation="MIVPYFESVRMRRFTRRELIIGGIALVLVLAGSLLAYARYFGPV
                     SATAQPATEFIVPPDADFDIVTRELKELGYIKSMTAFRIAYLSSGGMREIREGGYMLS
                     SSMDAWTVAELLSRPPYLAWITFPPGWRKEQIAEYLAKKLNWDPEEKRHFIEVDTAPS
                     PELVEGVYFGDTYLIPSDQDPAQVAARLRDRFNEVFAPLASQTIEKNVTWTETLIMAS
                     LIEREAAKNDKRLVSGILWNRINDGMRLQVDASLQYIRGQEGNWWPVPRPEDKDLDSP
                     FNTYRNSGLPPHPIANPSLESIEAALNPEDTDCLYYLHDNDGEIHCSATYAGHRANVE
                     RYLR"
     gene            complement(11034..11462)
                     /locus_tag="VGE48_00485"
     CDS             complement(11034..11462)
                     /locus_tag="VGE48_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041889.1"
                     /translation="MGAGVLLGLWLLFITCFYSLEPTEFGISRNMLTGTVSQQSRTGY
                     HMKPPWVFVSVIDTRPTRVCLTSSAHAAFNCRLVRFVGSEWRAFVRTEGFRLYWWSNR
                     LSFNSGYREEYRGMKDIMRGYAFSTEHYPFLEIIREYQTQ"
     gene            complement(11495..>11799)
                     /locus_tag="VGE48_00490"
     CDS             complement(11495..>11799)
                     /locus_tag="VGE48_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041890.1"
                     /translation="SIGTVDTRPMQVCINANARVLNCKLVEFNPDGLPVFLSWHGRKY
                     WESASLDEILKSYAFDGTGRSYPFLTILRELKNEEVDVTQLDRMAPVLPATPAPAQ"
BASE COUNT         2568 a         3301 c         3518 g         2412 t
ORIGIN      
        1 cgtcataagt gccgacagtc tccgggtgct ctaagagaga gtgtgctcta cgagcgcggg
       61 accttcgtcc cgcgctcgta ctatactgag tgcatgttta ctcctgaggc taacaagagg
      121 tttggtcgca tgcgtctcgc gcccgtgctc cttgcggtgc ttatggtgct tattggcgct
      181 gtatctgcct tctacgtttt ctctcaggta gaccgtgcag ggcgcgagca catccttgag
      241 cgtgtagcca cgatttcagt agccgtaccg caggaagagc tgcgagagct ctcaggaacc
      301 gaggacgatc ttggcacgcc ttcgtacgag acgctcaaac tattccttac aaagatgcgc
      361 tccgtaaacg gagacgcgcg gttcttgtac gtgattggaa agaatgcgga aggtaatctc
      421 ttcttctatg cagactcaga gcctgccgaa tccgctgact attcgccgcc cggtcaggtc
      481 tactacgagg cgacccccgc gatgcaggcg ctctttgaag acggcatacg gcgtaccgaa
      541 ggtcctgatc aggatcgctg ggggctatgg atctcaggat acgcaccggt tgtagatgag
      601 gcgggtaccg tagttgcgat gcttggcatg gatcttcctg ccagcagcta ccttggagat
      661 gcaatggcat acgcgtcact cccgcttctc atctcgcttc tccttatgct cggcatcata
      721 gccaccgagc gtacccgtag gcgcgagacc gcatacctcg agcagaaggc agaattcctc
      781 tcgatcgcat cacatgagat tcgtacgccg ctcacgggta ttcgctgggc gatcgagggc
      841 cttttgaagc gtcagaaccc acctcttaac ccaaagactc gcacggtact cgccctcgta
      901 catgaaagct gtctcggtct catcggccgt gtgaacaatc tcctggacct tactgcgctt
      961 gagggtggga atactgcgag gcttcgtccc gaggctatcg acctgcgtga tttcgtagaa
     1021 gatattgcgg atagtcttgc gctctcagca caagaacgac acgtatctat tgatgtggac
     1081 ggttcattcg ctgaagcagg tactttgacg gctgatcgtc agatgctcca tcacgcgctg
     1141 ttcaatctcc ttacgaatgc ggtgaagtat acccgcgaag ggagtgtcgt acgtatccgg
     1201 tacgctagag tttctggcat gcaccagctc tcagttgttg accaaggaaa gggaatagcc
     1261 gcggaggatc agcagaagat attcgccggg taccaccgca ccaaggaagc gatccgttca
     1321 ggagcatacg gcactggtct cgggctctat cttgttaaaa aggcggcaga gcttcatggg
     1381 ggtaccgtaa ccgtatcctc cgttctgggg gagggatcaa cgttcactct gtcacttccc
     1441 ttgaacgcct agtgctcgag gcgctcgttg accagcttca caacactgtc gatctggcgg
     1501 tccgacttca agaggtacgc aacggcccct tggctcatgg cccgcgcgac gtagtccatg
     1561 tcgttcgcgt tgctgagaac gatgacggga gctttatggt caggcgcttt ctcacggatc
     1621 tcttcgagca tttccacgcc gtccttgcga ggcatcatca cgtccatcac aaccgcatcc
     1681 ggcttctcag agagatagag cgaaaccccg tcctctccgt tggttgctga aagcaccgta
     1741 tagcccgcgt cttcgaactg atgcgtgagc gtgacgagca gaagcgtatc gtcttctaca
     1801 ataaggactt ttttgggtgt gctcatatgc cagaagtgta ccaccgggcc tctatccccg
     1861 gattttcata tggggaagtt ttcttcatga ggtcttaatc ggtaccatgg gaaggttctt
     1921 ccgtagcggc ggacattcgg ccgccttaac tttctagaaa ccatgacaaa attcggtact
     1981 ctcgttcttt tagcagcgtt tggctttggt gcggcaggcg ccgtacatgc gcagggcgta
     2041 aatcttgatg tcggaaccgg gattgatgtc tccgccacca gctccgtaat ggaggctgat
     2101 gtgatggcag acaccgaaac cagcctttcg agcgatgccg gtacctcgac cgaggcagga
     2161 agcgcaacac ttgctgaatt ctccatcgac cgcaacgacc ttacagaagg taccgtgtac
     2221 tcggttactc gcgcggagga cgtcatgacc ccggcaagcc ttgagtcgta cgtcggctcg
     2281 ctcgttgaac gcgatgatcg ggtagaggaa attgagcttg ctaacggtga gcttgtgatc
     2341 cgttacaagg cggaggcgaa gttcctcggc ttcattccgg gcagcatcac catccgcgcg
     2401 gaagtatctc ctgagggcga ggtacgtgtg tcgtatccgt ggtactcgtt cctcatggca
     2461 aaggctgaga gtcgcgcgga cctcgaggcg cgtctcaacc atgaccttga tgtgatcgca
     2521 gacgacctta ctgaggatat tgagatcggt tctggagcaa acgtccagac cgtgccaatg
     2581 aacgattcgc gccgcatggc acttatcgtc gagcgccttc gctcatcgct ctacgcgcag
     2641 ggaagcgcag aggctcgtac ctaacagagt tctaaatgca aaaggcccgc atcatgcggg
     2701 ccttttgcta ttttgtgaac ctagcgcgta cctcctttcc tggccagcgg tggtagagga
     2761 tcgggatgag gacgagcgtg agaatcatcg accacgcaag gccgaacatg atggagaacg
     2821 cgaggggtcc ccagatgcct gatgcgtatg agagcgggat catacccacc actgtcacca
     2881 cggtggtgag gatgattggg cggaagcgcg tcgaggctgc ttcgaccacc acgtctgtga
     2941 gggacgcgtc tgtgcgctcg cttccaatac gcgcgatcga atccataagg ataatcgcat
     3001 gattgataat cacacctgca agcgcgatga tgccgagcat cgaagggaac gagagcgcgg
     3061 agttcgtgat gaagagtcct gcgagcacgc ccaccaagga aagcgggatg atgaggagta
     3121 agtagagcga gtgacggaac gaattgaact caagcacgag gatcgcgagc atgagcgccg
     3181 ctccagcaag aagggcgata cccatctcgg caaacgactg gttcacgtcc tcgctctcgc
     3241 cgccgatctt catctcaaca ccttcaggaa gctccacgga ctccatcttt tcggtgaacg
     3301 tagaggcgat ctgaattggg tttccgcctg ttgcggcatc tgcggtaagg gtcgcgatac
     3361 gggtaccgtc ctcgtgacgg ataaccgcgt tcgcgggctc atatgcgatg gtcgcaatcg
     3421 atgagagcgg taccggaccg cgcgagcctg gaaccgtgag gctacggacc gcatcaatgg
     3481 tggcgtttgt ggtctcggat ggatcgcgga agccaggatt aagatcgagc ttagtgcgta
     3541 cctcgatgtc gttcgcgcct gcacggatat cggttgcgtc cgtgccaaag agggcactgc
     3601 ggagcgtatc tgcgactgca agcggcgaca caccaagctc agcagccttt gcagaatcga
     3661 gacgtaccac gaactcagca ccatcgtcct tcatggaggt gtcgacgttg gttgtaccag
     3721 gaacctcaga gagtacgcgt gcgccctggt cgaccgcggt gccaagcgct gcgagatcgt
     3781 caccaacgaa ggtaacggag atagcggcac ctgttggcgg gcctcctgca ggctcggaca
     3841 caacgatacg cgcggacgtc acggacgcga gcgtctttcg taggtccgta acgatctcgc
     3901 cgcttgtctg cttccggtcc tcagcaaggt ttacggttat gtttgcgata tgcgagccag
     3961 cgccgccggt gctgctgaag aagcttgccg taccgacatc agatacgaag gattcgatgc
     4021 gcgggtcttc gtacaggagc tcctcaactt cgcggactgc gaggtctgta tccgtaaggg
     4081 cggtaccctg cggcttctcg atggcgatgg tcacgaagtc catatcttca gcagggaaga
     4141 acaccacctt cacgagtcca gtgataggaa gaaggaggga gagcacgaag aggccggcca
     4201 tggtccagag gaacttgttc tccttctggc gattcccgag catagagcgg agatacttct
     4261 cgtaccatgc cttcgcgcgc tcgttgtatt cctcctggcg cttctcaagc gcggacggct
     4321 ccttcttgat cacgtagagc gccaagagcg gcacgaggcc gagtgccacg aagatcgatg
     4381 cgaggagcac gaagatgacc gtgaatggga tcgaagcaat gaacttaccc acgatgcctg
     4441 agaggaagaa caagggcacg aataccgcga ccgtggtcat ggtgcccgcg ataagcggcc
     4501 acgcgtactc cttgatagca gccacggccg ccttgtgctt gtcgcccagc tgcatgagac
     4561 gagtgtgcat ggcctcgact accacgatgc ccgagtccac aaggatgccg atcgcgagga
     4621 tgagcgagaa cagcgaaata aagttgatgg tgttgccact tgcggcaagg ccgatgaacg
     4681 caatcaagaa cgaaagcggg atcgatgctg ctgctaccaa cgactctctc catccgagtg
     4741 taagaaagag catgatcatg acgagcacca cggtctcaag gccgaccttc gaaagctcgg
     4801 ctaggtcgtg tgcgatatcg tctgcggcgt cgtaggtgat caccgtctca gtgcctgaca
     4861 gcggtcccgt ctcaaggtcc tcaagcagct tcttcacgtc gctaccagtt tcaaggatgt
     4921 tgccgccgcg gctcttgaac acggaaaggg tgagtgctgg agcggatggg gcaccatcaa
     4981 ccgacacgcg cgaataggtt gcagggtcgg cgagtccgtc cacgaccgtg gcgacgtccg
     5041 cgatgcgtat cggggtgccg cctgggcctg caagcgcaat cgcgcctacc tgggcagtgt
     5101 cggtgagacc cgcttcaaga cgtaccgagt aacggatacc gtcaacggtg atagcacctg
     5161 caggagcggc aacgccggac gcacgaagtg cgttcgtgac gtcgctaatc gagatgccgt
     5221 actgacgaag ctttgcctgc tcaaccacca cctgcacctg acgctcgcga acgccggaaa
     5281 cagagacgga agatacgccg cgtacgcgct cgatctcatc ggctaccgtc tcaccgagct
     5341 cggtaagctc agctggcgcg agttcgcccg cgatagagat gataagaatt ggctggtccg
     5401 cgaagttcac gtcagacacc accgggtcgt tcgcttcttc aggaagctca ggacgcacgg
     5461 tatccacctc gtccttgagt agctgaatag acttatcgat gtcagcgttt gcgttgaact
     5521 ctactgtgac ggaagaaaca ccgtctccag aagacgaggt aaccttcgat acaccttcga
     5581 tgccaagtac acggtcctcg accttgttcg tgataagagt ttcgatgtct tcagctgacg
     5641 cacccgggag tacggtcgtg accacaccca tggggatctg cacttcaggc gatgattcct
     5701 tcgggatcgc gagtacggca tagaggccgc ccagaataag cacgaccgca aggaggatcg
     5761 tgaaatggtg cttcgcgagg aagaatgtcc agaacttcgt cataagggtt agatgccggt
     5821 ctcagactcg acacgcactg cctcgccttc agagagtcca cgtgcatcct cgacgatgcg
     5881 caggtccttt gggaggtctg tacggatctc gatgcgatcc ccacgcactt cgccaacctc
     5941 aacctgatgt gcaaccaggc ggctatcctc tcctacagag aacacgatgc ggtctccggc
     6001 acgaagcttc acggcagcaa gcggaaggag tactggtcct tcaggttcag ctgcagtctc
     6061 ggttggtgca agatccggga gcgcgatatg gacggactgg ccgctcacaa gctcggtttc
     6121 gcccgtaacc gcgatgcgca cctcgatctg cttggtggtc gggtcgagtg caggcgccac
     6181 ggatgtgacg ataccctcgt aggcgccatc gatcataact tcgcctcctg caacaagaag
     6241 gtcacgatta tcctcggaca catatgcgac gatctcaagc gcgccattct gcgcgacggt
     6301 cgcaacgtgc tgcattgggg tgacgtaatc accgacacga ataggaagga agttcacggt
     6361 gccgccgatc ggcgcgcgca ctacagtgcg ctcgagcgcc gcctgtgccg cacgcaggga
     6421 accaagcgcc tgcttcacgc tcgcgtctgc gcttgcggtt gcggacacgg tgccgctcct
     6481 aagacctgcg cgagcactcg cgatacttga aagcacactg ttcacgccgg tgcgggcaga
     6541 agcgagcgcg ctcaactgag ctgctgttgc gcgcgagtca cgctcgttcg ctgcagctgc
     6601 gagttcgtta gtgaaggcct gcacctgacg cgcgatggac tcggcttcat caagaagtgc
     6661 ctgcgggctg cgcgagtttg cggtcgcgag gtttgcttcg aaggagcgca taagtgaatc
     6721 gatacgtgcg cgctcacgcg aaagacgcgt agcggtgttc gcgttcgggt agataaggag
     6781 gtcaggaccg actgctgtag gaccgccgaa gaacgcatca atatcgttct cgacgatagt
     6841 atcaagtgtc gtgtaggcgt cgcggtattc gtcacgagct gattcctgcg tgtccggaag
     6901 ggactgcgcg ctgcgcgctg ctacggctgc gtcatacgca ccttctgcct gaaggacggc
     6961 tgcgcgctcg gcagcgttct caagctctgc gatgacgaat ccagccggaa cgctcgcacc
     7021 gatgcgggtg cgaacgtttg tcacggtgcc gccgttcttc gcgaggatgt ttgcctcagt
     7081 cacggagcgg acggagccga tgaggtccac cgagcctccg gtgcccgaaa gctcgcccac
     7141 ggtagcgagc gacacaacac gaccatccgg aagcgtctcg ggctttgcgc tgctgcgggc
     7201 aagcgagata cctgcgatca aaagcgccgc aagtgctacg gcgatcacga tttgctgcca
     7261 gcgcttgagg ccgcggtatc ggcgccaaag cgccttcgaa cgtacggaaa cgagagaagc
     7321 gagcgtgcga agacgcttta taagtgcatc gatattcata ggcataagat tataacacaa
     7381 actacgacat gtcaacaata tgcggtcaag ggagtgccct ggaagtgcta tgctaagacg
     7441 catgaaaaac ttccataagc acgtgtcctc gcaccactcg ttcatgtacc tctgggtcgt
     7501 cggtgccatg ctcgtccttg ggggacttat ctggtcctac gtggcatcac accctacagg
     7561 aatcatcgac gggcagcatg agggtgatgt ccgttctaca gtagcggcct tcgggaatca
     7621 gctgagcgcg gtatcccttt tgtcaccgga cgcagcagac cagatacgtg ctgcatacgg
     7681 gccatacgtc tcagaggagc tgcttgcttc gtggatagag aatccgggta ctgcaccagg
     7741 acgcgctaca tcgagcccgt ggcctgacca tatagaggta gagcgcgtca caatgaacga
     7801 cacggatgcg tacgatgtgt ccggacgcat catacttacc gcaagtaccg gagacgcggg
     7861 aagtgttccg gtattcctaa cggttgccaa caccgagcat ggctatcgca tcacggcgta
     7921 tgcagaaggc acaaacgatc cagtgccagt cgtttctgaa ggtcgcgtaa cgctcgcgct
     7981 tggcgagacg cagacgcttt caggcgtctc gataacaccg gtccgtgtta tcgaagacag
     8041 tcgttgtccg atggatgcga tgtgtgtgtg gcagggaaca gcgcgtgtcg agatcagact
     8101 cgaggacggg atgggacaga gtgtcatgcc aatcgagctt tccggcgaac cggttacgag
     8161 cgagattgcg accatttggc tcgagagtgt ggagccgtat ccgatggcaa gccagccgac
     8221 cgaagacgca tcgtatcgat tctcgttccg cgtagaggcg cgctagctgc atgacgcagt
     8281 ccgaagcgct ttccatattg cagacgggcg cgaacgtctt cttaacggga gagcctggga
     8341 gcggcaagac gcataccgtg aacgcgtatg tcgcgtggct cagggcgcgc ggcatcgagc
     8401 cgtccataac cgcatcaacc ggcatcgctg cgacgcatgt gggcggtatg acgcttcatg
     8461 cgtggagcgg catcggcatc gcggagcgca tgtctgaaga attgctcgac gcgatatcga
     8521 gcaaagagca tgttgcaaag cgcctccaga aggcgaaggt gcttatcatc gatgagatat
     8581 cgatgctctc agggaacgtg ctgtcgatgg tggacgaggt tctacgcacc gtacgtcata
     8641 gcgaccgtgc gttcggagga ttgcaggtgg tgctcgttgg agattttttc cagcttccgc
     8701 ccgtgtcacg caccgaggtc tcttttgcgt tcgacgctgc tgcgtggaaa gcgctcaatc
     8761 cggtcgtgtg ctatctggaa gaacagcatc gccaggacga tacgacgttc ctgtcggtct
     8821 taagcgcaat cagggcggga gagtgggatc atacgcacgt atcgctcata acggcacgcg
     8881 aagccgagta tgacgagctc gcagaaggcg tcccgcagct cttcacacat aacgccgacg
     8941 tggatcggat aaatcagacc gaacttgcaa agctccccgg atcgccacac acattcgtca
     9001 tggagagcgc aggcgggaag gtgcttatcg agtcattgaa gcgcggatgc ctctcacctg
     9061 agtctttggt gctcaaggag ggggcggtcg taatggctac gaagaataat ccggtcgcag
     9121 gctatgccaa cggaacgctt ggcacggtca tcggatttga acgcggcacg gggtatccga
     9181 tcatcgagac gcgtgacgga cgcgagatcg caatcgcgcc ccaggactgg gcggtcgagg
     9241 aaggaggaaa ggtgcgcgcg aagataagcc aggtgccact tcgtcttgca tgggctatca
     9301 cggtccataa gagtcaggga cagagccttg atgccgcagc catggatctc tcgcgggcct
     9361 tcgagtacgg gcaggggtac gttgcgctct ctcgcgtgcg cacactctcg ggcgtacatc
     9421 ttctcggatg gagcgagaac gcgcttgcga tacatccgcg tgtcgcagcg tttgataagg
     9481 agctgaagga tgcatcagaa gcagcgcaac gggcatttgc agcactagaa gcatccggag
     9541 agcggatacc gcttgagcgg aacttcataa ctgctatggg cggtacgctc gaggctgctc
     9601 ccgtcggaga gcgtcctgag aagcgcacga cgtatgacga gacgctcgcg cttctcgaag
     9661 agggacgctc gctcatggat atcgcgaagg ttcgcaaact cacgcttggc actatcgcgg
     9721 accatgtagg aaagctcatc gaagccggac gtctcgatac agagagcctc gaagagcagc
     9781 tcccgggcag gcttcgcggt gcgctcccga agatacacga agcgttcgat gcgctcggcg
     9841 ccgagaagct cacaccagtg cattcgttcc tcaaaggaaa gtactcgttt gatgaactga
     9901 agctcgcacg agcgctctac cgaggatagg gctggtacaa tactgactac atgatcgttc
     9961 cgtatttcga gtccgtccgt atgcgccgct ttacgcggcg cgaacttatc atcggcggaa
    10021 ttgcgctcgt gcttgtactt gcaggatcct tgcttgcata cgcccggtat ttcggtccgg
    10081 tctccgctac tgcacagccg gcgaccgagt tcatcgttcc gccggatgcg gacttcgaca
    10141 ttgtgaccag ggaactgaag gagcttggct atatcaagag catgacagcg ttccgcatcg
    10201 catacctgag ctcaggcggc atgcgcgaga tccgcgaggg aggatacatg ttgagttctt
    10261 ccatggatgc atggacggtt gcagagcttc tttctcgtcc tccgtatctc gcatggatca
    10321 cgttcccgcc tggctggcgc aaggagcaga tcgcggagta tctcgcaaag aaactcaact
    10381 gggacccaga ggagaagcgg cattttatag aagttgatac cgcgccgtct ccagaacttg
    10441 tcgaaggcgt ctattttggt gacacctatc tcatcccttc ggaccaagac cccgcacagg
    10501 tggctgcacg tctgcgtgac cgattcaacg aagtgttcgc gccactcgcg tcacagacca
    10561 ttgagaagaa cgtgacctgg accgagaccc ttatcatggc gtccttgatc gagcgcgaag
    10621 ccgcgaagaa cgacaagcgt ctcgtctcag gcatcctctg gaaccgcatc aatgacggca
    10681 tgcgccttca ggtggacgca tcgctgcagt acatacgcgg acaggagggg aattggtggc
    10741 cagtaccacg tccggaagat aaggatctcg attcgccgtt caatacgtat agaaacagcg
    10801 gactcccgcc gcatccgatt gcaaatcctt cgctcgaatc gatcgaagca gcactgaacc
    10861 ctgaggacac agattgtctc tactatctgc atgacaacga tggtgagata cactgctcgg
    10921 cgacctatgc gggacatcgg gcgaacgtgg aacggtattt gcggtaaata taaaagaata
    10981 atgccactaa atcagaaggg ccccgcgatt tgactcgcgg ggccctgaga cgcttactga
    11041 gtttgatact cccggattat ctccaggaac ggatagtgtt cggtactgaa cgcgtatccg
    11101 cgcatgatgt ccttcatacc ccggtattcc tcgcggtatc cggagttgaa ggacagacgg
    11161 ttcgaccacc agtacaggcg gaagccttcc gttcttacga acgcgcgcca ctcgcttccg
    11221 acgaatcgga caagcctgca gttgaaggcg gcatgcgccg acgacgtcag gcaaacgcga
    11281 gttgggcggg tgtcgatgac ggacacgaag acccacggcg gcttcatatg atagccggtg
    11341 cggctctgct gagacaccgt cccggtgagc atattcctcg agatgccgaa ttcggtcggc
    11401 tcgagggagt agaagcaggt gatgaacaga agccagagcc ccaagaggac ccctgctccc
    11461 atgaacacct gcgccttgct cggccgaagc gacgtcattg cgccggcgcc ggcgtggccg
    11521 gcaagaccgg cgccatccgg tccagctgtg tcacgtcgac ctcctcgttc ttgagctccc
    11581 gcaggatggt caggaatgga tagctccggc ccgtaccgtc gaacgcgtag ctcttgagaa
    11641 tttcatcgag cgacgccgac tcccagtact tacggccgtg ccaggagagg aacaccggca
    11701 ggccgtccgg attgaactcg acgagcttgc agttcagtac ccgcgcgttc gcattgatgc
    11761 agacctgcat gggccgagtg tcgacggtgc cgatggact
//
LOCUS       DASWXU010000017         9752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_882318, whole genome shotgun sequence.
ACCESSION   DASWXU010000017 DASWXU010000000
VERSION     DASWXU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882318"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /gene="rpsB"
                     /locus_tag="VGE48_00495"
     CDS             <1..651
                     /gene="rpsB"
                     /locus_tag="VGE48_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY1041891.1"
                     /translation="GAHFAQVKSRRHPTMRPFVLGAKSNVEIFDLKKTDEQLAKAKSV
                     MAALARDGKVVLVVGGKREAQDGVKAIGKRLGVPFVAGRWLGGTLTNFTEIKKRIDRL
                     ADLSQKRDNGELAKLYTKLERIRIDREIEKLTVRLSGLTSLQRRPDAMVIVDTRHEAH
                     ASREARALGIPVIGVMSSDCDLKDATYPVVVNDASRDTIALVLDELASAYESGLKG"
     gene            704..1285
                     /gene="tsf"
                     /locus_tag="VGE48_00500"
     CDS             704..1285
                     /gene="tsf"
                     /locus_tag="VGE48_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEY1041892.1"
                     /translation="MDISLDTVKVLRDKTGVSIMQCRKALEEAEGDMDKAEVILKKRS
                     GAAADKKADRELGAGAIGTYVHDGVVGAMVLLSSETDFVSKNEEFGDLARAIAMHVAA
                     TNPIFLSSDEISEDKKAAALAIFQEEVKDKPAEMQEKILEGKMASYFKDQVLLEQSYI
                     RDDSKTVRDLLNEASQKFGERVEISKFARFSAR"
     gene            1319..1735
                     /locus_tag="VGE48_00505"
     CDS             1319..1735
                     /locus_tag="VGE48_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041893.1"
                     /translation="MIHLIFIGVSVLLLGGFFLLIALERNTEKRVLGSLRTKLDRRVS
                     RAAFIATHVDWGAFARHLAGTALERVLHDLAHTILQMVRATERILTRTVKTLRERRGM
                     PVAEEENRDAARPLARGIDRVRTALRNARKAPKNKA"
     gene            complement(1781..2200)
                     /locus_tag="VGE48_00510"
     CDS             complement(1781..2200)
                     /locus_tag="VGE48_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY1041894.1"
                     /translation="MRACCIIKPSAVTYRDEIVAALRSEGISIISEKTVTYTEPLVHA
                     LYDHMDTKAREAITHRLKGKDGIALLLEVATIARLLEVVGHDSDPRRCHPRSLRARFA
                     EDASEERMGDASWWENAVHRPIDEREALRDLSLLFPI"
     gene            2301..2373
                     /locus_tag="VGE48_00515"
     tRNA            2301..2373
                     /locus_tag="VGE48_00515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2334..2336,aa:Thr,seq:tgt)
     gene            complement(2428..3111)
                     /locus_tag="VGE48_00520"
     CDS             complement(2428..3111)
                     /locus_tag="VGE48_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041895.1"
                     /translation="MPLATRNALLALAITVAIVGTIVAAINHLDRQRIAELDAIQAQL
                     ATDTLSIETQFALLEDAPCEDLSVGTDLTQEVSLLGDRLGATESRLGSDNPEVIRIKK
                     QYTLLQIRDYLLTKRIADTCDVEPVTALYFYSNEPGACESCDRASYALSYLRQTYPGL
                     RVYSFDYNLDLGALKTLIAVEKIDERFPAFVIEGERSYGFEDLETFEERFPEELFEMA
                     SSTDAEGEE"
     gene            complement(3135..4373)
                     /locus_tag="VGE48_00525"
     CDS             complement(3135..4373)
                     /locus_tag="VGE48_00525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1041896.1"
                     /translation="MNLEVLAYPFVFLGLYFEVFMLLTFLSQPAKERRLRGAATNTPK
                     VAIIVPCWNEQDTIGGTVNSLLALDYPKDKLEIILVNDGSTDNTKEVMDAYVGNPQIT
                     VIHKENGGKHTAINLGIEHAKDAEFVGCLDADSFVAPDALREIIGSFDSPKVAAATPA
                     MSVFQPKNLLEQMQNAEYIIGIAARHALSAVNGIYVTPGPFSFYRRSVIEEIGGFRHA
                     HQAEDMEMALRMQRAGYEIENAPRARVYTKVPRSVPKLIKQRTRWTTGFLRNVMTDYR
                     DLVGNPKYGTLGLMVLPLGFVAIVGGAFLFFVALLALVQGVYRSLSLTSGIPLTYTLT
                     PRVALDWFYVPVTFILLASLVVGVLSISLVLIGRHVSNTPAKVALGIVGYTFIYAFIA
                     PFWLMRSMYDVLTGKRRAWR"
     gene            4450..5178
                     /locus_tag="VGE48_00530"
     CDS             4450..5178
                     /locus_tag="VGE48_00530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY1041897.1"
                     /translation="MDSGRLHPLSADIREIAAIFARMGFGIAYGPELEDEHHNFDALR
                     VPADHPARDMQDTFWTKEETPRAPRTHTTSVTIRCLEEMAKNGQTEARMIAPAKVFRN
                     EATDATHEAQFYQLDGFAVGPKGTVTLAHLKGTFDYFYRAYLGEDAKVRFRPSFFPFV
                     EPGVEIDVWYEVPGKKGRWLETGGAGMLHPEVIKNAGLDPEKIGGFAFGGGIERLLMV
                     KYGIPDVRAFHNGDIRFTYGFDPK"
     gene            5182..7086
                     /locus_tag="VGE48_00535"
     CDS             5182..7086
                     /locus_tag="VGE48_00535"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY1041898.1"
                     /translation="MKVSKKWLEEYFDSALPDTEALSNALTFHAAEIEESTDDMLDVK
                     VLPDRAAYLMSHRGVALELGAALGMPLKSDPLRKELPVFPETDELAVSLDEEGKCNRY
                     MGALVKGVKVGASPAWLKEALESVGQRSINNVVDATNYVMLNIGQPLHAFDAKKLSVR
                     DGTYAIAVRGASEGEAFTSLTGEEYALPEGTLVVADGNNNAVLGVAGVKGGKEAEVDE
                     QTMDLIIEAANFDGTQVRRTAQKLKLFTDASSRFQNRPSPALAAYGMRDVLTLITDIA
                     GGEVTGVTDIYPNPEQPETVSASLFEVNAVLGTSFSMDEVRAALIRLSFSFEEVEDRF
                     IVLVPFERRDLSIPEDLIEEIGRTIGYDSIAGIQLPVLAGEPEQARYRGIERVKDFLV
                     ERGYAEVSTPSFAGEGEVLLANPLQEEKPYLRASLIENVKDALTRAAQLAPRVIGPEQ
                     FVRLFEVGSVFTAKGEHLVLSMGAVPLSGKPAKAAELLQQDVLALSQDLVGAPLAPRY
                     SLDGQVMELDLGALNLEKLGEDYAPTAVHMGAFRPYSQYPFALRDIAVWTPEGTAESE
                     VANAIVKDAGEFLVRLDCFDRFEKEGRISYAFRLVFESMERTLADTDLDPAMQRVADS
                     LNAKEGWEVR"
     gene            7575..9680
                     /locus_tag="VGE48_00540"
     CDS             7575..9680
                     /locus_tag="VGE48_00540"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HEY1041899.1"
                     /translation="MAKAGEEKPKKAAAPAAAKASKEKAYDASSISVLEGLEPVRKRP
                     GMYIGTTGPDGLHHLIWEIFDNSRDEAMGGFADDIEVALLPGGYVRVVDNGRGIPVGI
                     HPKTKVSALETIMTTLHAGGKFGGEESGYKVSGGLHGVGASVVNALSTHTKVIVHKDG
                     GIHMQEYEIGKAKAKVKQIGKTDKHGTIVMFRPDESIFKDGINWNWEKIVAHLRQQAY
                     LVKGTRITVIDAREVTVLNAEEAYYLRHEDIDCPSMSFFFEGGLRSLIGFYNKHQTPV
                     HKNVFYVDAAEGDVQVEVAFQYVDDISPRLMAFANNIYNPEHGTHVTGFKTALTRTLN
                     SYGRSAGILKESEENLTGDDVLEGITAVVSVKMPEIQFEGQTKGKLGSVEATGATASV
                     FGTALAMFLEENPDDGKAIVNKGILALKARKAAKAAKDSVLRKGALDGMTLPGKLADC
                     QTKAAEDSELFIVEGDSAGGTAKTGRDRRTQAILPLRGKILNIERARLDKMLASEQIK
                     NLVVALGTAIGDIFDLNKLRYHKIIIATDADVDGAHIRTLLLTLFYRHFRPVIDGGFI
                     YIAQPPLYKIKKGKETFYAYSDEEKFKILGDDAAAAVEEADEEQGTEEEGGEAPEEEV
                     TTKKGAKVSIQRYKGLGEMNASELWETTMDPGNRVLKRVTVEDGAEADRIFDILMGTD
                     VASRKSFIQSNAKLANLDV"
BASE COUNT         2138 a         2696 c         2949 g         1969 t
ORIGIN      
        1 ggcgctcatt ttgcgcaggt gaagagccgt cgccatccga ccatgcgtcc gttcgtgctc
       61 ggcgcgaagt ccaatgtcga gatcttcgac cttaagaaga ccgatgagca gctcgcgaag
      121 gcgaagtccg taatggcagc actcgcacgt gacggtaagg tcgtacttgt cgttggcggc
      181 aagcgtgagg ctcaggacgg tgttaaggct atcggcaagc gcctcggcgt tccgttcgtt
      241 gcgggtcgct ggctcggcgg tacgcttacg aacttcacgg agatcaagaa gcgtatcgat
      301 cgcctcgctg acctctcgca gaagcgcgac aatggcgagc tcgcaaagct ctacacgaag
      361 ctcgagcgca tccgcattga tcgcgagatc gagaagctca ccgttcgcct ttctggtctt
      421 acgtcacttc agcgtcgtcc ggatgcaatg gttatcgtcg atacccgcca tgaggcacat
      481 gcgtcccgcg aggcccgcgc gctcggcatt ccggttatcg gcgtgatgag ctctgactgc
      541 gaccttaagg acgcgaccta cccggtggta gtaaacgatg catcccgcga caccatcgcg
      601 cttgtgctcg acgagctcgc atcagcgtac gagtctggcc tcaagggcta atctccgttc
      661 ccgctcaggt tctcttactt acattcaata gcgctttata cctatggaca tctctctcga
      721 taccgttaag gtcctccgcg acaagactgg cgtttccatc atgcagtgcc gcaaggcgct
      781 tgaggaggcc gagggcgata tggacaaggc cgaggttatc ctgaagaagc gctctggtgc
      841 agcggctgac aagaaggcag atcgcgagct cggtgcgggt gctattggca cctacgtcca
      901 tgacggcgtg gtaggtgcaa tggtgcttct ttcgtctgag actgatttcg tatcgaagaa
      961 cgaggagttc ggcgatcttg cgcgcgctat cgcgatgcat gtcgctgcta ctaatccaat
     1021 cttcctctcg tctgacgaga tttctgagga caagaaagca gcggcgcttg caatcttcca
     1081 ggaggaagtt aaggacaagc cggcagagat gcaggagaag atcctcgagg gcaagatggc
     1141 gtcctacttc aaggaccagg tgctgctcga gcagtcctac atccgcgacg actctaagac
     1201 ggtccgcgac ctccttaacg aggcttcgca gaagtttggc gagcgcgtcg agatttccaa
     1261 gttcgcgcgc ttctcagctc gctagccgaa ggccgggaga ccggccttct cgcgtaccat
     1321 gatccacctc attttcattg gcgtctcagt ccttcttctc ggaggattct tcctgctcat
     1381 cgcgcttgag aggaacacgg aaaagcgcgt tctaggctcg cttcgtacga agcttgaccg
     1441 tagggtctcg cgtgccgcgt ttatcgcaac ccatgtcgat tggggtgcct tcgcaagaca
     1501 cttggcaggc actgcgctcg agcgtgtgct ccatgacctt gcgcacacca tcctccagat
     1561 ggtccgtgca accgagagga tccttacgag gactgtgaag accttgcgtg aacgccgcgg
     1621 catgccggtt gccgaagaag agaatcggga tgcggcacgt ccccttgctc ggggtatcga
     1681 tcgcgttcgc acggcactca ggaacgcacg aaaggcgcca aagaataaag cataagacac
     1741 aaaacacgga cccgacatcg ggtccgtgtt tgcatcagtg tcagataggg aagagaagcg
     1801 agagatcgcg cagcgcctca cgttcgtcga tagggcggtg caccgcgttc tcccaccacg
     1861 atgcgtctcc catgcgctct tctgacgcat cttccgcgaa ccgtgcgcgc aaggagcgcg
     1921 ggtggcagcg acgtggatcg ctgtcatgtc ctacgacctc aaggagtcgc gcaatggttg
     1981 cgacttcaag gagaagcgca ataccatcct tgcctttaag acgatgggtg atagcctcgc
     2041 gcgccttcgt atccatatgg tcgtagagcg cgtgtacgag tggctcggtg taggtgaccg
     2101 tcttctcgct gataatcgag atcccttcgc tcctgagcgc agcgacgatt tcgtccctgt
     2161 aagtgactgc tgaaggcttg atgatgcagc acgcacgcat ggcttctctc ctagcgaacg
     2221 ggtgtccgca ggaactatac gaagcagctg atagaacttt cctggattaa acataaagtt
     2281 tccggtatcg tagcctagac gccgggatag ctcagttggt agagcaacgg ttttgtaaac
     2341 cgtaggtcgc gagttcaagc ctcgctcccg gctcagacaa aaggaaaggc gcccgaggtt
     2401 acctcgggcg cctttcgtat ttctttctta ctcttcgcct tcagcgtctg tcgagctcgc
     2461 catctcgaac agctcctcag ggaagcgctc ctcgaacgtc tcaagatcct cgaatccgta
     2521 tgagcgctcg ccctcgataa cgaacgccgg gaagcgctca tcgatcttct caaccgcgat
     2581 aagggtcttg agcgcaccca gatcgaggtt gtaatcgaag gagtacacgc ggagtcctgg
     2641 gtacgtctgg cggaggtagg agagggcgta gcttgcgcgg tcgcagctct cgcaggcccc
     2701 tggctcgttc gagtagaagt agagcgcggt tacgggctct acgtcgcagg tgtccgcgat
     2761 gcgcttcgtg aggagataat cgcggatctg aagcagcgtg tactgcttct taatgcgaat
     2821 aacctcagga ttgtcgcttc caaggcgcga ctcagttgcg ccgaggcgat cgccaaggag
     2881 cgacacttcc tgcgtcaggt ctgtgcctac cgaaaggtct tcacacggtg cgtcctcaag
     2941 gagcgcgaac tgcgtctcga tggagagggt atcagtcgca agctgcgcct ggatcgcgtc
     3001 gagctcggca atacgctgac gatcgagatg attgatcgcc gcgacaatcg taccgacgat
     3061 cgcgacggta atcgcaaggg caaggagagc gtttctggta gcgagaggca taaatctaca
     3121 attacgtata aggactagcg ccaggcacga cgcttgccgg taagcacatc atacatggaa
     3181 cgcatgagcc agaatggggc aataaaggcg tagatgaagg tgtatcccac aataccgagt
     3241 gctacctttg caggggtgtt cgagacgtgg cgaccgatga gaacgaggga aatagagaga
     3301 actcctacta cgagactcgc aaggagaatg aacgtgacgg gtacgtagaa ccaatcgagc
     3361 gcgacgcgag gcgtaagtgt gtaggtaagc gggatgccgc tcgtaagcga gagcgaacgg
     3421 tacacgccct gcacgagcgc aaggagcgcg acgaagaaca ggaatgcgcc gcccacgata
     3481 gccacgaagc caagcggaag aaccatgagg ccgagggtgc cgtactttgg gttgcctacg
     3541 aggtcccggt aatcggtcat cacgtttcga aggaatccgg tagtccagcg ggtgcgctgc
     3601 ttaatcagtt tcggcaccga gcgcgggacc ttggtataga cacgtgcacg cggagcgttc
     3661 tcgatctcgt agcctgcgcg ctgcatacgg agcgccatct ccatgtcctc ggcctgatgc
     3721 gcgtggcgga agccgccgat ctcctcgatc acggaacggc ggtagaagga gaacggccct
     3781 ggggtcacgt agatgccgtt cacggcagac aacgcgtggc gcgcggcaat gccgatgatg
     3841 tactcagcgt tctgcatctg ctcgagcaga ttcttcggct ggaacacgga catggcagga
     3901 gtagccgctg ccaccttcgg gctgtcgaag gagccgatga tctcgcggag cgcgtccggc
     3961 gctacgaatg agtctgcatc aagacagccc acgaactccg catccttcgc atgctcgatg
     4021 ccgagattga tcgcagtgtg ctttccgccg ttctccttat gaatcacggt tatctgtggg
     4081 ttgccgacat acgcgtccat cacctccttc gtgttgtcag tagacccgtc gttcacgagg
     4141 atgatctcga gcttgtcctt cggatagtcg agcgccaaga gcgagttcac ggtcccaccg
     4201 atggtgtcct gctcgttcca gcaagggaca atgatcgcta ctttcggggt gtttgttgcg
     4261 gcaccacgaa gccgacgctc cttcgctggc tgcgagagga aggtgagaag catgaagacc
     4321 tcaaaataga ggccgaggaa cacgaacggg taggcgagta cctcgaggtt catagatgcc
     4381 agagcgttcc cataagcata gcaaatacgc ctgttttttc taggttccag gctgtatact
     4441 gcgggaacaa tggattcggg acgcttgcat ccgctctcag cagatatacg cgagattgcc
     4501 gccatattcg cgcgcatggg gttcggtatc gcctacggtc ctgagctcga agacgagcat
     4561 cacaacttcg atgcgctgcg cgttcctgcg gatcatccgg cgcgcgatat gcaggacacg
     4621 ttctggacaa aggaagaaac gccccgtgca ccacgcacgc acaccacgtc tgtaacgatc
     4681 cgctgccttg aggagatggc gaagaacggg cagaccgaag cgcgcatgat cgctccggcg
     4741 aaagtgttcc gaaacgaagc aaccgatgcg acgcacgagg cgcagttcta tcagctcgac
     4801 ggatttgcgg ttggtccgaa gggaacggtg acgctcgcgc atctcaaagg aaccttcgac
     4861 tacttctatc gggcatacct cggcgaggac gcgaaagtgc gcttccgtcc gagcttcttc
     4921 ccgttcgttg agccaggcgt cgagatcgac gtctggtacg aggtcccggg aaagaaggga
     4981 cgatggcttg agaccggtgg cgccggcatg ctgcatccgg aagttatcaa gaatgctggc
     5041 ctcgatccgg agaagatcgg tggtttcgcg ttcggtggag gcatagagcg ccttcttatg
     5101 gtgaagtacg gcatcccgga tgttcgtgcc ttccataacg gcgacatccg cttcacatac
     5161 gggttcgatc ccaaatagca tatgaaggta tccaagaagt ggctcgagga atatttcgat
     5221 tcagcgctcc cagacacgga ggcgctttca aacgcgctca cgttccatgc ggcagagatc
     5281 gaagagagca cagatgacat gctcgacgtg aaggtgcttc cggatcgagc agcatacctc
     5341 atgtcgcaca ggggcgtcgc gcttgagctc ggtgctgcgc tcgggatgcc tctcaagtct
     5401 gatccacttc gaaaggagct tccagtgttc ccggagacag atgagcttgc agtctcgctc
     5461 gacgaagaag ggaaatgcaa tcgctacatg ggcgccctgg tgaagggcgt taaggtcggt
     5521 gcgtctccag catggctcaa ggaagcgctc gagtccgtag gacagcgctc catcaacaac
     5581 gtcgtggacg ccacgaacta cgtgatgctc aacatcgggc agccgctcca tgcgttcgac
     5641 gcgaagaagc tctccgtgcg cgacggcacg tatgccatcg ctgtccgcgg cgcttctgaa
     5701 ggagaagcgt tcacgtcgct tacgggagag gagtatgcgc ttccggaggg cacgctcgtt
     5761 gtcgctgacg gaaacaacaa cgcggtgctc ggtgttgctg gtgttaaagg tggaaaggaa
     5821 gccgaagtag atgaacagac gatggatctc atcatcgagg cagcaaactt cgacggtacg
     5881 caggtgcgtc gcactgcgca gaaactaaag ctcttcacgg atgcgtcctc acgcttccag
     5941 aatcgtccat ctcctgcgct tgctgcctac ggtatgcgtg acgtactcac gctcataaca
     6001 gatatcgctg gcggcgaagt aacgggcgtt acggacattt acccgaatcc tgaacagcca
     6061 gaaacggtgt cggcttcgct gtttgaggtt aacgcggtgc ttggcacatc gttctccatg
     6121 gacgaggtgc gtgcagcact catacgcctc tcgttctcct tcgaagaagt cgaggaccgg
     6181 ttcatcgtgc ttgtgccgtt tgagcgcagg gacctttcta tacctgaaga cctgatcgaa
     6241 gagatcggcc gtacgattgg atacgactcc attgccggga tacagctccc ggtactcgca
     6301 ggcgagccgg agcaggcacg ctatcgcggc atagagcgcg tgaaggactt ccttgtcgaa
     6361 cgcggatatg ccgaagtgtc cacgccatcg ttcgcaggag agggtgaggt cctgctcgcg
     6421 aacccgctcc aggaagagaa accatacctg cgagcctcac tcatcgagaa cgtgaaggat
     6481 gcgcttacgc gcgccgcaca gctcgcaccg cgcgtgatag ggccggagca gttcgtgcgc
     6541 ctctttgaag tggggagtgt gttcaccgca aagggtgagc atctcgttct ttcgatgggt
     6601 gcggtaccgc tctcaggaaa gcctgctaag gcagcagaac tccttcagca ggacgtgctc
     6661 gcgctttcgc aggaccttgt aggtgctccg ctcgcgccgc gctacagcct cgacgggcag
     6721 gttatggagc ttgatctcgg ggcgctcaat ctcgagaagc tcggggagga ctacgcaccg
     6781 acggcggttc atatgggagc gttccgcccg tattcacagt atccgttcgc gcttcgcgat
     6841 atcgcggtgt ggaccccgga gggaacggct gagagcgaag tagcgaacgc tatcgtgaag
     6901 gatgcgggag agttcctcgt acgtctcgat tgtttcgatc ggtttgagaa ggagggtagg
     6961 atctcctacg cgttccgtct cgtattcgag tccatggagc gcaccctagc tgatacggat
     7021 ctcgatcctg cgatgcagcg tgttgccgac agcctgaacg cgaaggaagg ctgggaggtg
     7081 cgctaacaag aaaccgcccg acctttgagg ccaggcggca ctcgtagtcg ggcggtcagt
     7141 attcgcagcg gatttcccgt agggtgatag tgaattcttt gggtgcgatc accatcatcg
     7201 cccttccatc gctcatgtgt accatgcgca gtgaggacga atcgagtgtg gtcgggacgg
     7261 gtgaacccgg attcagcgga agggccatcg tcgtcctgag gctcggagaa tagaagcagt
     7321 gaacgttctc aacgttcctc aggtgttcag gaacctccat cgacgatggg ctgcagcccg
     7381 ccgttagaag gcaggcggta aggccgatca atctcagcat gggctttctc ctttcaatgc
     7441 accaagtttg gcacaggctt tctaatgttt atgatgtcat ttatacatca atttgtcaag
     7501 atttagaatc ctaaacaatg aggcataaac tgccccctgg agccctatca tcgtgctata
     7561 ctgaagctaa cgatatggcc aaggcagggg aggaaaaacc taagaaagcg gctgctcctg
     7621 cggccgctaa ggcgtcaaag gaaaaggcat acgacgcgtc ctcgatctcc gtgctcgagg
     7681 gtcttgagcc ggtccgtaag cgaccaggca tgtacatcgg taccacgggt cccgatggtc
     7741 ttcatcacct catctgggag atcttcgata actcgcgcga cgaggccatg ggcgggttcg
     7801 cggacgacat cgaagtcgcg ctccttccgg gaggctatgt gcgcgtcgtg gataacggac
     7861 gcggtatccc ggtaggtatc catccgaaga caaaggtctc agcgctcgag acgatcatga
     7921 ccacgctcca tgcgggcggc aagttcggcg gcgaggagtc cggctacaag gtctcgggcg
     7981 gtctgcacgg cgtgggtgcg tcggtagtga acgcgctctc gacccacacg aaggtcatcg
     8041 tccacaagga tggaggcatt cacatgcagg agtacgagat cggtaaagct aaggcgaagg
     8101 tgaagcagat cggcaagacc gataagcacg gcaccatcgt catgttccgt ccagacgaat
     8161 ctatcttcaa ggatggcatc aactggaact gggagaagat tgtcgcgcat ctgcgccagc
     8221 aggcgtacct cgtgaaaggg acgcgtatca cggttatcga cgcgcgcgag gtaacggtcc
     8281 tcaatgccga agaggcgtac tacctccgcc atgaggacat tgattgtcct tccatgtcct
     8341 tcttcttcga aggaggcctt cgttccctca tcggtttcta taacaagcac cagaccccgg
     8401 tgcacaagaa cgtcttttac gtagatgcgg cggaggggga cgtgcaggtt gaggtagcgt
     8461 tccagtatgt ggacgacatc tcgccgcgcc tcatggcgtt tgcgaacaac atctacaacc
     8521 cggagcacgg tacgcacgtg acgggcttta agactgcgct cacgcgtacg ctcaattcct
     8581 acggccgctc cgcaggtatc ctcaaggaga gcgaggagaa cctcactggc gacgacgtgc
     8641 tcgagggcat cacggccgtg gtgtccgtta agatgccgga gatccagttc gagggtcaga
     8701 ccaagggcaa gctcggctcg gtagaggcta cgggtgcaac tgcgtcagtc tttggtacgg
     8761 cgcttgccat gttcctcgag gagaatccgg acgacgggaa ggcgattgta aacaagggta
     8821 tcctcgccct taaggctcgc aaggccgcga aggccgcgaa ggattccgtg ctccgcaagg
     8881 gcgcgcttga cggcatgacg ctgccgggca agctcgccga ctgccagaca aaggcagcag
     8941 aggattccga gctcttcatt gtggagggag attccgcagg aggcactgcc aagaccggcc
     9001 gcgaccgccg tacccaggcc atcctcccgc ttcgcggcaa gatcctcaac attgagcgcg
     9061 cgcgtctcga caagatgctc gcgagcgagc agatcaagaa tctcgtggtg gcgctcggca
     9121 cggcgatcgg cgacatcttc gacctcaaca agctccgcta ccacaagatc atcatcgcga
     9181 ccgatgccga tgtggacggc gcccatatcc gcacgcttct ccttacactt ttctatcgcc
     9241 acttccgtcc ggttattgac ggcgggttca tctacatcgc gcagccacct ctctacaaga
     9301 taaagaaggg caaggagacg ttctacgcat actcggacga ggagaagttt aagatccttg
     9361 gagacgacgc agcggctgcg gtcgaagaag cggatgagga gcagggcacc gaagaggagg
     9421 gcggcgaggc acctgaggaa gaggtaacca cgaagaaggg tgcgaaggtc tccatccagc
     9481 gctacaaggg tctcggagag atgaatgcgt cagagctctg ggagaccacc atggacccgg
     9541 gtaatcgcgt gctgaagcgc gtcacggttg aagacggcgc agaagcagac cgtatcttcg
     9601 atatcctcat gggtactgat gtggcgagcc gcaagtcctt cattcaatcg aacgcgaagc
     9661 tcgcgaatct cgacgtgtag gttcctaata ctagaaaagc cccgctcata ggagcggggc
     9721 ttttctgtaa ggagaaggga aacgacgtcc ta
//
LOCUS       DASWXU010000018         5042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_830819, whole genome shotgun sequence.
ACCESSION   DASWXU010000018 DASWXU010000000
VERSION     DASWXU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830819"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VGE48_00545"
     CDS             <1..758
                     /locus_tag="VGE48_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY1041900.1"
                     /translation="LGLPVAKGGAVSSLRAAKKLYKALTPPVIAKPSSGSASRHTILH
                     IDTEEELARAFKIANQVAPTVVIEEELNGPVYRATVVDGKFAAALRRDQPLVVGDGKR
                     TIAELVEEANKHPGRQGPYFHHMKLDEAAKEELSWQGYTFKSVLPEGEKAILHQKINW
                     SVGGTTCDVTDEVHPDNVALFEETARVLKAPIVGIDFIIPDITRSWKEQERLGILECN
                     SMPFFDNHHLPFEGKPRNVAALIWDMNEPKKRG"
     gene            complement(1047..1970)
                     /locus_tag="VGE48_00550"
     CDS             complement(1047..1970)
                     /locus_tag="VGE48_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1041901.1"
                     /translation="MHDLIIIGGGPAGVAAGVYASRKRLKSVLIAEEIGGQSTVSDGI
                     ENWVGTVRIAGEDLKKSFKAHLEAYKGDVLEVIEGDRVESLTRSEGGFTATTRDGKSF
                     TGRAVLLASGASRKKLLVPGADKFENMGVTYCASCDGPMFSGMDVAVVGGGNAGLETA
                     AQLLAYAKSVTLIHRHPTFKGEAITVEKILQHPNLKTVMNAEPTEVLGEKFVSGLTVK
                     DRTSGEEATLPVTGVFVEIGVIPNTGFAKDLVELDEVGRVVTNARDQHTSVEGVWAAG
                     DCTDELYHQNNIAAGDAVKALEDIYQWLARN"
     gene            complement(2025..3323)
                     /gene="ftsZ"
                     /locus_tag="VGE48_00555"
     CDS             complement(2025..3323)
                     /gene="ftsZ"
                     /locus_tag="VGE48_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY1041902.1"
                     /translation="MSKRVEPEVETFARIRVVGVGGSGGNAVNHMVNSKVKGVEFISV
                     NSDAQDLHRSLAKRKIHIGKNLTRGLGTGMNPELGKRAAEETRQEIQEALQGSDMVFI
                     TCGMGGGTGTGAAPVVARIAKEVGALTVGVVTRPFTFEGLHRKELAERGLHDLKKEVD
                     AFIVIPNDKLLAIVEAQTSAKNAFSMADEILRQAVEGVSDIITTPGEINTDFNDIKAI
                     MEGAGPALMGIGIAEGDRRAQDAAAQAVNSPLLDVSITGAKGVLFVVAGSEDLGILEV
                     QEAAKVVSDSVDPSAKVIFGIMRDEKLKKGQVRIIVIATGFPEGESAHSQGGIERSLF
                     AMGPVEQEEVRGKIYNEAVAQPRPEPTPAPEPTPAPEPAPAPKPVMERRREEPVVTAQ
                     PKPREEATMKTVVPPSAQNQTEDEDEAWGAIPSFLRRHKK"
     gene            complement(3421..4593)
                     /gene="ftsA"
                     /locus_tag="VGE48_00560"
     CDS             complement(3421..4593)
                     /gene="ftsA"
                     /locus_tag="VGE48_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY1041903.1"
                     /translation="MSRRIATGIDIGTHQTKVVVVEEVQGENGAELHIIGTGISESRG
                     MRHGYVVDAEDAAESVRLARMQAEQIARVPIRKGFLAIGGVSLDEVRASGEAIISRAD
                     QEITDLDLEKVLETAREAARPSFLNRTVLHEIPIEYRVDGAKSLGEPIGLKGTRLTAD
                     YLFITCLTQHAEVLTAATEAADIEVIDRMASPLAGSYVTLAKDQKMKGCVLANIGAET
                     VSIVVYDEGLPISVKVFPIGSTNITDDLALGFKVSLEDAERLKLGRLGGAMYPRKKVE
                     EIVTARFRAMFELIDKHLKQLGRRGNLPAGIIISGGASGQGSISDIAKGTLNLPTRIA
                     DLKLSSDTKVKDATWAVAYGLALWGLTGDTETPKKKGMQMGEFATTLQKFFKQFLP"
     gene            complement(4565..4990)
                     /gene="ybeY"
                     /locus_tag="VGE48_00565"
     CDS             complement(4565..4990)
                     /gene="ybeY"
                     /locus_tag="VGE48_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY1041904.1"
                     /translation="MTTLDTRNFTRQTPPTFPFKDALHAALPGWEMSLVFAGETRAQN
                     MNIALRGKNYVPNVLSYESGNKSGEIVICLEVAKRQAPSYGLSYRDFVGLLFIHGCMH
                     LKGMQHGDTMDKRERSLLQRFTRANLSRTSNVPTHRNRH"
BASE COUNT         1062 a         1447 c         1408 g         1125 t
ORIGIN      
        1 agctcggcct cccggttgca aagggcggcg cagtctcgag ccttcgcgcc gcaaagaagc
       61 tctataaggc gcttacacct ccagttattg caaagccctc atcaggctct gcgtcgcgcc
      121 acaccattct ccatatagat accgaagagg agcttgcgcg cgcatttaag atcgcgaacc
      181 aggtagcacc tacggttgtt atagaagagg agctgaatgg tcctgtgtac cgagcaacgg
      241 ttgtggacgg gaagtttgcc gcagcgctca ggcgcgacca gccgctcgta gtcggagacg
      301 gcaagcgcac catcgcggaa ctcgttgaag aagcgaacaa gcacccagga cgccagggcc
      361 cctacttcca tcacatgaag ctcgacgaag cagccaaaga agagctttcc tggcagggct
      421 acacgtttaa gagcgtgctt cctgaaggag agaaggcaat cctccaccag aagatcaact
      481 ggagtgtggg tggcacgacc tgtgacgtga ctgatgaagt gcacccggat aatgttgcgc
      541 tcttcgagga aacggcacgc gtgctcaagg cacctatagt ggggatcgat ttcataatcc
      601 ctgacattac gcgctcttgg aaagagcagg agcgcctcgg catcctcgaa tgcaactcca
      661 tgcccttctt cgacaatcat cacctcccgt ttgaaggaaa gccgcgcaat gtagccgcgc
      721 tcatctggga catgaatgag cccaaaaaga ggggataaac cttcatttcc ttttcagagg
      781 ggtgcttggt aaaatttagg ggttggtcga attgccagcc aattaacttt ggatggctgt
      841 atttttcgct ctgcgaagga tagagccatt ctccaacaca catggatcag gaaatggaca
      901 tgacccagga gggcgcagag gaggaagctc cgatgatgcc tgaggagggt atgggcgaag
      961 aggcagctcc ggctggcgaa gacgaggcag ctgcataagc tctcgtacca aaggaaaaag
     1021 ccgcatcaac tgatgcggct ttttccttag ttccttgcga gccactgata gatatcctcg
     1081 agcgctttaa ctgcgtctcc tgccgcgatg ttgttctggt gatagagctc gtcggtgcag
     1141 tctccggcgg cccagacgcc ttcaactgat gtgtgctggt cacgcgcatt cgtcaccacg
     1201 cgtccaacct catcaagctc tacgagatcc tttgcaaacc cggtgtttgg aatgacgccg
     1261 atctcaacga acacgcctgt aaccgggagc gttgcctctt ctccagaggt gcgatccttc
     1321 acggtaagtc ctgagacgaa cttctcgccg agaacctctg ttggctcagc gttcattacg
     1381 gtcttcaggt tcgggtgctg caatattttc tcaaccgtaa tcgcttcacc cttaaacgtc
     1441 gggtggcgat ggatgagggt aacagatttc gcatatgcaa gtagctgcgc ggccgtctca
     1501 aggccggcgt ttccgccacc cacaaccgcg acatccatac ccgagaacat cgggccatca
     1561 caacttgcac agtatgtgac gcccatattc tcgaacttgt ccgcacctgg caccaaaagt
     1621 ttctttcggc tcgcaccaga agcgagtaat accgcgcgtc cggtaaagga cttaccatcg
     1681 cgcgtagtgg ccgtaaagcc tccctcagag cgcgtaaggc tctcgacacg gtctccttcg
     1741 ataacctcaa gcacgtcgcc cttgtaggcc tctaggtgcg ccttgaagga cttcttcaga
     1801 tcctcgcctg cgatgcgcac cgtgccaacc cagttctcga ttccgtctga gacggtgctt
     1861 tgcccgccga tctcttcggc gatgaggact gatttaaggc gcttgcggga ggcgtagacg
     1921 ccagcggcga cacctgctgg tccgccgccg atgatgatga ggtcgtgcat ggcgtcagta
     1981 tagcaaaatc ccctggacca tgggtccagg ggattttgtt tgcgctactt cttatggcgc
     2041 cttaggaagc ttgggatcgc gccccatgcc tcatcctcgt cctcagtctg gttctgggct
     2101 gaaggcggga ccacggtctt catagttgct tcctcgcgcg gctttggctg cgcggttaca
     2161 accggttcct cgcgacggcg ctccataact ggttttggtg cgggagcggg ctcaggtgcg
     2221 ggagtcggct ctggagccgg agtaggctca gggcgtggct gtgctaccgc ttcgttgtag
     2281 atcttgccgc gcacctcctc ctgctcaacc ggacccatcg caaagagaga gcgctcgatg
     2341 ccgccctggc tgtgcgcgct ctcgccctct gggaagccgg tagcgatgac gatgatgcgg
     2401 acctggccct tcttgagctt ctcgtcgcgc atgataccga agatgacctt tgcggacgga
     2461 tccactgagt cggacaccac cttcgccgcc tcctggactt cgaggatacc gagatcctcg
     2521 gagcctgcga ccacaaagag tacgcccttg gcgccggtga tagatacgtc gaggagcggg
     2581 gaattgacgg cctgtgcagc agcgtcctgt gcgcgacggt cgccctctgc gataccgatg
     2641 cccatgagcg cgggacctgc gccttccatg atggccttga tgtcgttgaa gtcggtattg
     2701 atttctccag gggtggtgat gatgtcagag acaccttcta ctgcctggcg aagaatctcg
     2761 tcggccatgg agaacgcgtt ctttgcgctg gtctgcgcct caacgatcgc aagaagcttg
     2821 tcgttcggaa tcacgatgaa cgcatctacc tccttcttga ggtcgtgcag accgcgctct
     2881 gcgagctcct tgcgatgaag accctcgaag gtgaacgggc gagtcacgac accaacagtg
     2941 agtgcgccca cttctttggc aatccgtgca acgacaggcg ctgcaccggt gccggtgccg
     3001 ccacccatgc cgcaggtgat gaagaccata tcggaaccct ggagtgcttc ctggatttcc
     3061 tgacgagtct cctcagcagc acgcttgcca agttccgggt tcatgccggt accaaggcca
     3121 cgggtaaggt tcttgccgat gtggatcttg cgcttcgcga gggagcggtg gaggtcctga
     3181 gcatcagagt tcacggaaat gaattccacg cccttaacct tggaattcac catgtggttc
     3241 acggcatttc cgccggatcc accgacacct accacgcgga tgcgggcgaa ggtctcgact
     3301 tctggttcaa cacgctttga catatggagc tattaacggg tcccctaata acgtggcctt
     3361 atcatacaag tacaaaatca gcatgcaaat gctgacaaaa cagggaactg tgctcttgtt
     3421 ttatggcaaa aactgcttga agaacttctg cagggtcgtc gcgaactctc ccatctgcat
     3481 gcctttcttc ttcggcgtct cggtgtcgcc cgtaaggccc cagagtgcaa ggccatacgc
     3541 aactgcccag gttgcatcct tcaccttcgt gtcgcttgag agcttcagat ccgcgatacg
     3601 agtaggaagg ttcaaggtac ccttcgcgat gtcgctgata gaaccctggc ccgacgctcc
     3661 gcctgagatg atgatgcctg ctgggaggtt gccgcgacgg ccaagctgct tcaggtgttt
     3721 gtcgataagc tcgaacatgg cgcggaagcg tgccgtaacg atctcttcta ccttcttgcg
     3781 cggatacatt gcgccgccaa gacgtccaag cttcagtcgc tccgcatctt caagggatac
     3841 cttgaaccca agtgcgagat catctgtaat gttagttgaa ccaataggga acacctttac
     3901 ggaaatcgga agaccttcgt catacaccac gatagagacg gtctcggcgc cgatgttcgc
     3961 gagtacacag cccttcatct tctgatcctt cgcgagtgtc acgtaggaac cggcaagtgg
     4021 ggatgccatg cgatcgataa cctcgatgtc cgcagcttcg gtagcggcgg ttagcacctc
     4081 cgcgtgctgc gtgaggcagg tgatgaacag ataatcagcc gttaggcgcg tacctttaag
     4141 accgattggc tctccgagcg attttgcgcc gtcgacacga tactcgatcg gaatctcatg
     4201 cagcactgtc ctatttaaaa agctcgggcg tgctgcttcg cgcgcggtct caagtacctt
     4261 ctcaagatca agatctgtaa tctcttgatc tgcacgcgag atgatcgctt cgcctgatgc
     4321 acgcacctcg tcgagtgaca cgccgccgat agcgaggaat cccttgcgga tcggcacgcg
     4381 tgcgatctgc tccgcctgca tgcgggcgag tcgcacgctt tcagcggcgt cctccgcatc
     4441 aactacgtag ccgtggcgca tgccacggct ctcagagatg cccgtaccga tgatatggag
     4501 ttccgcaccg ttctcgccct gcacctcttc gaccaccacg actttcgtct ggtgtgtgcc
     4561 gatatcaatg ccggttgcga tgcgtcggga cattcgaggt acgagatagg ttagcgcgag
     4621 taaaacgctg cagaagcgag cgttctcgtt tatccattgt atccccgtgc tgcatgccct
     4681 tcaaatgcat gcatccgtgg ataaagagca atccgacgaa gtcgcgatag gaaagtccgt
     4741 atgatggcgc ctggcgcttc gccacctcga gacagattac gatctcgccg ctcttgtttc
     4801 cggattcgta tgacagcacg ttcggaacat agttcttccc gcgaagcgcg atattcatat
     4861 tttgtgcacg ggtctctccg gcgaacacca aagacatttc ccaaccggga agcgctgcat
     4921 gaagggcatc cttgaagggg aaggtaggag gcgtctggcg cgtgaagttt ctcgtatcga
     4981 gcgtagtcat gggggaatga tagcaagaaa gcgccctaag gcgctttctc ttacttcttg
     5041 cc
//
LOCUS       DASWXU010000019        12108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_222950, whole genome shotgun sequence.
ACCESSION   DASWXU010000019 DASWXU010000000
VERSION     DASWXU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222950"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..684
                     /locus_tag="VGE48_00570"
     CDS             76..684
                     /locus_tag="VGE48_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041905.1"
                     /translation="MSFKAYIGAFFALLFFVWAPQAHAKVTNADVLDGVNEWRAAYAT
                     ANHLDAAEILVYERRISIDPPTTLMVCGKSHTFTKGDVVWNVCRKHLEEQAAAAAKPA
                     TEANPVSNAGGTFAAKGDVTDQDAYGKESLDEKSDRMSKALAYILITLVAFAIFIFWL
                     CRESKKSAQRYEAKRVLRGTYRELPLESPYKDSPPQASRSPP"
     gene            939..1469
                     /locus_tag="VGE48_00575"
     CDS             939..1469
                     /locus_tag="VGE48_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041906.1"
                     /translation="MKTQTAAAGAMLVLLTSAMPAKAQEAYNPTNCVASLRYTTGGTI
                     DPQNLRVGETVTDGTFVTLVYPTMTPEAICRIFAPGRLQIQQAEAAAIQAEGLRVAAV
                     TQGEDDRNFRIRAEKDPVRRDPYAAMGAASVATLLAVFVVGLFWRVVMKILKGIFAHL
                     KRRNNRGRFSKRPYAH"
     gene            complement(1529..1954)
                     /gene="rplI"
                     /locus_tag="VGE48_00580"
     CDS             complement(1529..1954)
                     /gene="rplI"
                     /locus_tag="VGE48_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY1041907.1"
                     /translation="MKVVLLKDVKNTGRAGAVIECSDGHALNFLIPRKLAAPATAANV
                     KQAELRAQQDKDRRALDVKLVEERIASLAEERIVIKKKANEKGHLYDAVDAADIAKAT
                     ELPVDAIRIEKPFKETGTFDVPVAAGEGFGKISVVIEAE"
     gene            complement(1993..3243)
                     /locus_tag="VGE48_00585"
     CDS             complement(1993..3243)
                     /locus_tag="VGE48_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY1041908.1"
                     /translation="MQEEEAQATKPAFAKVAKLGVAFALVLAVGFAGGVAAGAEGKSV
                     FSNLPILGDGLDATPDSEADLTDFWKAWNALDQRFVETHATGTPPSIKEKLWGAIQGL
                     AASYGDPYTVFMPPNEAKQFKDDISGNFEGVGMEVGVKDGVLTVISPLKGTPAERAGL
                     RSGDAILTIDGTSTDGLSTDQAVKLIRGKKGTTVVFNILREGSVSEISVVRDTIAVPT
                     IETSQESGVFIISFYSFTANSTQLFSKAMAEFRASGSKLLLIDLRGNPGGYLEAAVTI
                     SSHFLPKGAVVVTEDFKGKQENVVHRSRGIGGLPEDTKVAILIDQGSASASEIMAGAL
                     QDGDAATLIGAKSFGKGSVQELVEINGGALKVTIARWLTPAGQSISDGGLTPDIEVKR
                     TREDAEAERDPQKARAIEFLTTGR"
     gene            3324..4241
                     /locus_tag="VGE48_00590"
     CDS             3324..4241
                     /locus_tag="VGE48_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY1041909.1"
                     /translation="MITRHMRGKTVWVDLESPTEEELALVMREFDIDERIHEEIATPT
                     PYPLTIPFPGYTYLILHFPIAGSEDGTRSQEVDFIVGKQFLITARYEPVESLHNLHRI
                     LEAEELLSIPEHAVKTGAFLERVISRLYASISTEIEQVGKRLERIERDIFSGREQHAV
                     RTISEAGRVLLRFETALARHKEPLADFLAALATPAYFGSSFEEHAAHIEARRTHAAAL
                     VASLRAVAQELRITNDSLLTASQNQVTKTLTVMAFVALPLTLIASVFGMNAENMPLIH
                     HEQGFWIVLGLMALAGLSLFTYFRLKKWL"
     gene            4257..5717
                     /gene="cysS"
                     /locus_tag="VGE48_00595"
     CDS             4257..5717
                     /gene="cysS"
                     /locus_tag="VGE48_00595"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY1041910.1"
                     /translation="MKWLTSLFSPKSEPVTSDAPVLLQNTLTDRKEVFSPIKAGVATM
                     YSCGPTVYSRAHIGNLRSYVFADVLARMLSQAGYQTRRVINITDVGHLTDDADSGEDK
                     MEKGAREAGMSASDIAARYTKLFIEDIRALGVDADDISFPRATDYIEEQIDIVKLLEE
                     KGYTYRIKDGIYFDTRAFPNYGILDHAGDKLREEAFAEIGRRIAANNEKRHPADFALW
                     KFSPLRSSRQQEWNSPWGRGFPGWHIECSAMSRALLGQPFDIHTGGIDHIPVHHTNEI
                     AQSEAAYGKPLARFWMHNAFLTIENRKISKSLGDDVYVSDIKERGFSPLSLRYLFLQA
                     HYASPLSFSWESLEAADAALKRLSRLAYTIKAEADERTRPSEWSRRIMALLRDDLSTP
                     QALALLWEAVKDEDLTREEQLGAIEAAESVLGLGLLSVPRAEIPAHILALVSEREAAR
                     TARDFAKADTLRIHIENSGYAVEDGPSGPVVTPKAG"
     gene            5731..7095
                     /gene="dnaB"
                     /locus_tag="VGE48_00600"
     CDS             5731..7095
                     /gene="dnaB"
                     /locus_tag="VGE48_00600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY1041911.1"
                     /translation="MAADPTLRVPPQSMDSERALLGSLLLKPDAIHDVSDLIRADSFY
                     AEKHRHIFECMRELTERGDPIDMLSLGERLQAKGVLERAGGRAYIAELASSSPTPGNY
                     AHYAELVARKHVMRSLIDVAHEITEAAYDEGGDTAQVLDHAEKSIYAIGNASATHKFV
                     AIKDKVDDAWERIETLSKNGGNVRGVPTGFPDLDNLLSGLHKSDLIILAARPSMGKTS
                     LALDIARNVAVKHDTPVGIFSLEMSSEQLVDRMLSAESFVNSWKMRTGAIHAEEDFGK
                     IRDALETLSKAPIYIDDKPAQNILSMRAVARRLKRERGIGLIVVDYLQLMAPTNTKAS
                     DSMVQQVTEISRSLKQLAREIEVPVIALSQLSRAVEQRGGKPRLSDLRDSGSIEQDAD
                     VVMFIHREDKQNKESDRPNIAEILIEKHRNGPTGKVELYFDEKRTSFMPMDKTGYGEF
                     ADEF"
     gene            7099..7704
                     /locus_tag="VGE48_00605"
     CDS             7099..7704
                     /locus_tag="VGE48_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY1041912.1"
                     /translation="MRDRIDELSSAFNRFPGIGPRQAKRFVYHLLSVPQGDRTRLADL
                     IQNLAADVRQCGLCMRFAPTNHENLCNYCADANRDDSILMVLEKDQDLQAVERAGTYR
                     GRYFVLGGVITLSGKGAIRERDLVRVVEIRAKQGLREIVLALSATSEGEHTADRVREL
                     LAPLRGPITLSELGRGLATGSELEYADSATLSAALQNRKEA"
     gene            complement(7758..8006)
                     /locus_tag="VGE48_00610"
     CDS             complement(7758..8006)
                     /locus_tag="VGE48_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041913.1"
                     /translation="MTSADPERRVAVSKEWLLNELPKEDAEIWVPELERQVRVDRLYD
                     HEFHTVVSAGDVDTILRAPSTTWVDHKDYADEMARNAA"
     gene            complement(8061..8282)
                     /locus_tag="VGE48_00615"
     CDS             complement(8061..8282)
                     /locus_tag="VGE48_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041914.1"
                     /translation="MQKGIELEDEYKDGDLFRLHELAHEFPVEKRPDVIPEGYDYALH
                     FIVPAHVMGRFSEEAGWAEITGCTTCPRE"
     gene            complement(8430..8759)
                     /gene="rplU"
                     /locus_tag="VGE48_00620"
     CDS             complement(8430..8759)
                     /gene="rplU"
                     /locus_tag="VGE48_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY1041915.1"
                     /translation="MELAVIKTGGKQYVVSPGATVTIEKLGRGAATQDVKKGDTVTFD
                     QVLMTDNGTLSLGTPTLSGKTVTGTVQSVGRAKKVEVVKYKAKSNYLKRRGHRQPQIK
                     VKIDKIA"
     gene            complement(8805..9860)
                     /locus_tag="VGE48_00625"
     CDS             complement(8805..9860)
                     /locus_tag="VGE48_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY1041916.1"
                     /translation="MNAITIVIIVAIVMLLQGIGLYIVLRRRENDVPKDDGQGLMLLQ
                     NQMEKLERSLDARMHESSREIHETVRSQLGESSRLIRDITAEITSVKEIGRETGKFAE
                     QLKSLQDILKNPKQRGVLGEYYLETVLKNVLPPGMYKMQHMFENGEIVDAAVYVNDKL
                     VPIDSKFSLENYNRYVQAADADKAMYEKAFVTDLKNRITETAKYIRPEEGTMDFAFMF
                     IPSEGIYYDLLTNQVGAGDDENLIQRAAGKYKVIIVSPTSFLAYLQTVLQGLKALQIE
                     KQAEEIQKRVGELGKHLSAYETYYQKLGTALGTAVSHYNSGYKELGKVEKDVYRIAKE
                     KIGLEVELLDKPGSGEE"
     gene            complement(9857..>12108)
                     /locus_tag="VGE48_00630"
     CDS             complement(9857..>12108)
                     /locus_tag="VGE48_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY1041917.1"
                     /translation="ALATGNAVPADLRLVEVHGLTVNESALSGEWLAVEKDTAEAKED
                     APLVERSGMAYAGTLVAAGAGKGIVVATGGDTELGQIAAELGKKDESVTPLMRDIRQV
                     ARFILIVVCFVLIGIIALGLVRGLPLEETIFTAIALAVASVPEGLPAAVTVVLALGME
                     RILRSGGLVRNLLAAETLGTTSVILTDKTGTLTEGRMTLELFVGRTGYYADAFSGEAH
                     QVLSAAVIASNAYVEERPELPEDEKLVVHGRPIEAAIVLSGLKNGIAQPVLLSVSPRI
                     DELHFDSARRFGGMLVRGGTAYVTGAPELFLTSMRKVADADGEAHALSDKALSHFEDA
                     LSMAAQSGKRVIAVARIPNAGSAFPSEKELPELLAKGELLGLMVFSDVIRAEAKDAIR
                     DMQDAGARVLMLTGDNPETALSIARSVGIATDKDERAYTGAELSGLSDAELLEVLQNH
                     TVFARVAPSEKLRMANVLKDAGEVVAMTGDGVNDAPALRAAAIGIAVGSGTDVAKEAS
                     DLVLLNNGFSVITHAIKEGRRLRDNFKKIFTYMLSTNFSEVALITFSLIFGLPLPILP
                     TQILWSNLVEGGLMNFAFAFEPLYPSAMKRTPKDPETSRVISPKIVRLIMVIGAITSV
                     TLISVYCFLVFATELSLPEIQTLMFVAVSVNCIFMAFSMKSFGTPLWKLPLLSNKFLL
                     LALIASAGLLLAALYVPAIQTIVHVSPPTLLQVGMLVGFGLVNLATIEVAKWLVFIRP
                     QSKSAILPA"
BASE COUNT         2624 a         3634 c         3323 g         2527 t
ORIGIN      
        1 ctgggttaag tggcgcagga attgctgccg cacgggaccc ttgatcattc acactatcgc
       61 aagaaaggga cagtcatgtc cttcaaggct tatatcgggg cgttcttcgc ccttctgttc
      121 ttcgtctggg ctccccaagc ccacgcgaag gtcaccaacg ccgacgtact cgacggcgtc
      181 aacgagtggc gcgccgcata cgcgaccgcc aatcacttag acgccgctga gatactcgtt
      241 tacgagcgtc gcatcagcat cgacccgccg acgaccctca tggtctgcgg caagtctcac
      301 accttcacca aaggcgacgt ggtctggaat gtgtgccgga agcatctcga ggagcaggcg
      361 gccgcagctg ccaagcctgc gacagaagcg aatccggtat caaacgctgg cggcacgttc
      421 gctgccaaag gagacgtgac cgaccaagac gcatacggaa aggaatcgtt ggatgagaag
      481 agcgaccgca tgagcaaagc tctggcgtac atcctgatca ccctcgtggc tttcgccata
      541 ttcatcttct ggttatgccg cgagagtaag aagtctgcgc agcgctacga agcgaagcgc
      601 gtcctccgcg gcacataccg cgagttaccg ctggagagcc cctacaaaga ttcgccgccg
      661 caagcttcgc gctcaccacc ttgaccaatt cagggtttcg agccgccgat ccgcgctacg
      721 tacctgcaac aggtgcgttc ttccggaggc aacgacggag aggagctggc agaggtccag
      781 cataagcagt cttgcgatag agcatttcga aggccgtccc accaggacgg ccttttgacg
      841 ttcatgaagt tgattgagtt tcttagtttt agaggtatag tgtgcgaagt ttgaaccaag
      901 ggacctttag gtcccttctg ctctttgagg gggaatacat gaaaactcag accgcggcag
      961 caggagcaat gctggtgctg ctgacttcgg caatgcctgc gaaagcgcag gaagcctaca
     1021 acccgacgaa ctgcgtcgcg tcgctccggt acacaaccgg cggtacgatt gatccgcaaa
     1081 atcttcgggt aggcgaaacg gtcaccgacg gaacgtttgt gaccctggtc tatccgacca
     1141 tgactcctga agcgatctgc aggatcttcg cccccggacg cttgcagata cagcaggcag
     1201 aagcggctgc gattcaagcg gaaggacttc gcgtggctgc ggttacacag ggcgaagacg
     1261 atcgcaactt caggatccgt gcggagaaag atcctgttcg cagggacccg tatgcggcca
     1321 tgggagcagc ctcagtcgca accctgctcg cggtgttcgt cgtcggcttg ttctggaggg
     1381 ttgtcatgaa gattctgaaa gggatctttg cgcacctcaa gagacgaaac aaccgaggca
     1441 ggttcagcaa aagaccctat gcccactaag cccaaaacaa agcggcgcgc cctaaatgga
     1501 cgcgccgctc gtgcttttta tccaggcttt actcagcctc gataacgacg gaaatctttc
     1561 caaagccctc ccctgccgca accggcacat caaatgttcc ggtctccttg aacggcttct
     1621 caatgcgaat cgcatctact ggaagctcgg tcgccttcgc gatgtcagca gcatcgactg
     1681 cgtcgtacag gtgacccttt tcgttcgcct tcttcttgat gacgatgcgc tcctcagcaa
     1741 gcgaagcgat gcgctcctcg acgagcttta catcaagtgc gcggcgatcc ttgtcctgct
     1801 gcgcacgaag ctctgcctgc tttacgttcg ctgcagttgc tggagcagcg agcttgcgag
     1861 gaataaggaa gttgagggca tggccgtccg agcactcgat aactgcgcca gcacgtcccg
     1921 tgttcttaac gtctttgagt agtacgactt tcatatatag gcatcgtacc ggtaaagccg
     1981 gaacgaggca atttaccgcc cggtcgtgag gaactcgatc gcacgagcct tctgcgggtc
     2041 tcgctctgcc tcagcgtcct cacgagtgcg cttcacctcg atatccggcg taagaccacc
     2101 gtcggagatg gactgccctg ccggcgtgag ccaacgagca attgtcactt tgagtgcgcc
     2161 gccgttgatc tcgacaagct cctgcaccga gccctttccg aaggacttcg cgccgatgag
     2221 ggtagcagca tccccgtcct ggagcgcgcc agccatgatc tcagaggcgg acgcagaacc
     2281 ctgatcgata aggatcgcta ccttcgtgtc ttccggaagg ccaccaatcc cgcggctcct
     2341 atgcacgacg ttctcctgct tgcccttgaa gtcctcagta accacaactg cgcccttcgg
     2401 gaggaagtga cttgagatgg ttaccgccgc ctcaagataa ccgccaggat tgccgcgaag
     2461 gtcgatcaga agaagcttcg agccggaagc acggaactct gccatagcct ttgagaagag
     2521 ctgggtggag ttcgcggtga agctatagaa ggagatgatg aatactccgc tctcctgaga
     2581 ggtctcaatt gttgggaccg cgatggtatc gcgaaccaca gaaatctctg acaccgagcc
     2641 ctcgcgaagg atattgaata cgacggtcgt acccttcttg ccgcggataa gctttacagc
     2701 ctgatcggtc gagagcccat cagtcgaggt gccgtcaatc gtgagaatcg cgtcacccga
     2761 gcgcaggcct gcgcgctcag ccggcgtacc cttgagtggc gagataacgg ttagcacacc
     2821 atccttgacc cccacttcca tgcccacacc ttcaaagttg ccgctaatgt cgtccttgaa
     2881 ctgcttcgct tcgtttggcg gcatgaatac cgtgtatggg tccccgtagg atgccgcaag
     2941 tccctggata gcaccccaaa gcttctcctt gatcgaagga ggtgtgcccg tcgcatgggt
     3001 ttctacgaac cgctgatcaa gcgcattcca tgccttccag aagtcggtga gatccgcttc
     3061 tgaatcagga gtcgcatcaa gcccgtctcc gaggatcgga aggtttgaga acacactctt
     3121 tccttcagcg cctgcggcaa ctccacctgc gaacccaacc gcgagcacga gtgcgaacgc
     3181 aacgccgagc ttcgcgacct tagcaaatgc aggcttcgtt gcctgtgcct cttcttcttg
     3241 catatgcgtg cattctatca cgcacattct atccacggtc atgaagcgca aatgagaagc
     3301 gtacgccggt atactaggaa gtaatgataa cgaggcacat gcgcggcaag accgtctggg
     3361 tagacctcga atccccgacg gaagaggagc tcgcgctcgt gatgcgcgag ttcgatattg
     3421 atgagcgtat ccacgaggag atcgcaacac ctacccctta cccgctcacg atcccatttc
     3481 ctggatacac gtacctcatc ctccattttc cgatagcagg atccgaagac ggtacccgtt
     3541 cgcaggaggt ggacttcatc gttggcaagc agttcctcat caccgctcga tatgagccgg
     3601 tagagtcact tcataacctg catcgcattc ttgaagccga ggagcttctt agcattccag
     3661 aacacgccgt taagaccggt gcgttcctcg agcgtgttat aagtcgcctc tatgcatcga
     3721 tcagtacgga gattgagcag gttggaaagc gtcttgagcg catcgagcgc gatatcttct
     3781 ccggacgtga gcagcatgcg gtgcgcacca tctcagaagc gggacgtgtg ctgcttcgct
     3841 ttgagaccgc ccttgcccga cacaaggaac cgctcgcaga tttccttgca gcgcttgcca
     3901 cgcctgcata ctttggctct tccttcgaag agcatgctgc acatattgag gcgcgccgca
     3961 cccacgcggc ggcgcttgta gcctcactcc gcgccgtcgc acaggagctt cgcataacga
     4021 atgattcact tctcaccgct agtcagaacc aggttacgaa gaccctcacc gttatggcgt
     4081 tcgtggcgct tccacttacc cttatcgcct ccgtgtttgg catgaatgcg gagaatatgc
     4141 cactcatcca tcatgaacag ggcttctgga tcgtgctcgg actcatggcc ctcgcaggac
     4201 tctccctctt cacgtacttc cggcttaaga agtggctcta gggtagaata gggcgtatga
     4261 aatggctcac ctccctcttc tcgcctaaga gcgagcccgt aacgagcgac gcgccggtcc
     4321 ttcttcaaaa tacgcttaca gaccgcaagg aggtcttctc ccccatcaaa gcaggtgtcg
     4381 ccaccatgta ttcctgcggc cctacggtct atagccgcgc gcacatagga aacctccgtt
     4441 cctatgtgtt tgctgacgta ctcgcccgga tgctctcgca agctgggtac cagacccgta
     4501 gggtcataaa catcacggat gtcgggcacc tgactgacga tgcggactct ggcgaagaca
     4561 agatggagaa aggagcgcgc gaagccggca tgagcgcctc agatattgcc gcacggtaca
     4621 ccaagctatt catcgaggac atccgagccc ttggagtgga cgcggacgac attagcttcc
     4681 cgcgcgcgac cgactacatc gaggaacaaa tcgatatcgt gaagctgctc gaggagaagg
     4741 gctacaccta ccgcatcaag gacggcatct atttcgatac tcgtgcgttc ccgaactacg
     4801 gcatactcga tcacgcagga gacaaactcc gcgaagaggc gttcgccgag atcggacgcc
     4861 gcatcgctgc aaataatgag aaacgacatc ctgcggactt cgcgctctgg aagttctctc
     4921 cgttgaggtc cagtcgtcaa caggaatgga acagtccgtg gggtcgcgga ttcccaggct
     4981 ggcacatcga gtgttccgcg atgagtcgcg cacttctcgg ccagccattt gatattcata
     5041 caggtggcat cgatcacatc ccggtgcatc acactaacga aatagcacag tccgaggctg
     5101 cgtacggaaa gccactcgct cgtttctgga tgcataacgc cttccttacg atcgagaatc
     5161 gaaagatatc gaagtcccta ggagacgatg tgtacgtatc tgacatcaag gagcgaggtt
     5221 tctctccgct ttcgctcagg tacctgttcc tccaggcgca ctacgcgtcc ccactgtcct
     5281 tctcctggga atcgcttgaa gctgcggacg cggccctgaa gcggctttcg cgccttgcgt
     5341 acacgataaa agcagaagca gatgagcgga ctcgcccatc agaatggagc cgtcgtatca
     5401 tggcgctcct tcgggatgac ctatcgactc cgcaagcgct cgcgcttctc tgggaagcgg
     5461 ttaaggacga ggaccttacc cgagaggagc agcttggtgc tatcgaggca gcagaaagcg
     5521 tacttggtct tggtctcctc tctgtcccgc gtgcagaaat cccagcacat atactcgccc
     5581 tcgtctctga gcgcgaagcg gcccgaactg cacgtgattt tgcgaaggct gacacactcc
     5641 gtatccacat cgagaacagt ggatatgctg ttgaggacgg gccttccgga cccgtagtca
     5701 ccccgaaggc aggttgatag aatagaggga atggcagctg acccgactct ccgcgtaccg
     5761 cctcagagca tggattctga gcgcgcactg ctcggttccc tcctcttgaa gcctgacgcg
     5821 atccacgacg tctcggacct catccgcgcg gactccttct acgcggagaa gcaccgccac
     5881 atattcgagt gcatgcgtga gctcacggag cgcggtgacc cgatcgatat gctttctctc
     5941 ggagagcgtc tccaggccaa gggcgtcctt gagcgtgcag gtggacgtgc gtatatcgca
     6001 gaactcgcga gctcttcgcc gacccctgga aactacgcgc attacgcgga actcgttgcc
     6061 cgcaaacatg tcatgcgttc tttgattgat gtggctcacg agataacgga agcggcctac
     6121 gacgagggtg gcgacactgc acaggtgctt gatcacgcgg agaagtctat ctacgcgatc
     6181 ggaaacgcat cagccacgca caagttcgtg gctatcaagg acaaggtgga tgacgcctgg
     6241 gaacgcatcg agaccctttc gaagaacggc ggcaacgtgc gcggcgtccc taccgggttc
     6301 ccggacctcg acaatctcct ttcaggcctt cataagtccg acctcatcat tctcgcagcc
     6361 cgtccgtcca tgggtaagac gtcgcttgca ctcgatatcg cccgcaacgt cgccgtaaag
     6421 cacgatacgc cggtaggtat cttctcgctc gagatgagct ccgagcagct cgtagaccgc
     6481 atgctctcag cggagtcgtt cgtgaactca tggaagatgc gcacaggcgc gatccatgca
     6541 gaggaagact ttgggaagat ccgcgacgcg cttgagactc tctcgaaggc tcctatctac
     6601 atagacgata agccagcgca gaacatcctt tccatgcgcg cagttgcaag acgattgaag
     6661 cgcgagcgcg gtatcgggct tatcgtggtg gactacctcc agctcatggc accgactaat
     6721 accaaggcat ccgattctat ggtgcagcag gttacggaaa tctcccgctc cttgaagcaa
     6781 ctcgcccgtg agatcgaagt gccagttatc gcgctctcac agctctcacg cgccgtggag
     6841 cagcgcggcg gcaagccgcg cctctcggac ctccgcgact cgggttccat cgagcaggac
     6901 gcagacgtgg ttatgttcat ccatcgcgag gacaagcaga acaaggagag tgatcgtccg
     6961 aacatcgctg agatcctcat cgagaagcat cgtaacggcc ctaccggcaa agtagagctc
     7021 tacttcgacg agaagcgcac cagcttcatg cctatggaca agaccgggta cggagagttc
     7081 gctgacgagt tctagcctat gcgtgatcgc atcgacgagc tctcaagtgc attcaatcga
     7141 ttcccgggca ttggcccccg gcaggcaaaa cgcttcgtct accacctcct ttccgtgccc
     7201 cagggagacc gcacgcgtct tgcggatttg atacagaacc tcgctgctga tgtgcgacag
     7261 tgcgggctct gcatgcgctt tgcacccacg aatcacgaga acctttgcaa ctactgcgcg
     7321 gacgcgaacc gggacgattc catcctcatg gtgctcgaga aggatcagga cctgcaggcg
     7381 gtcgagcgcg cgggcacgta tcggggacga tacttcgtac ttggcggggt tataaccctc
     7441 tctggcaagg gtgcgatacg cgaacgggac ctcgtacgcg ttgtcgagat acgcgcaaag
     7501 caggggctcc gagaaatcgt gcttgcgctc tcagcgacct ctgaaggcga acacaccgca
     7561 gacagagtcc gggaactcct cgcgccgcta cgaggcccta ttaccctctc agagctcgga
     7621 cgtggccttg caaccggaag cgaacttgag tacgcggatt ccgccaccct ctccgccgcg
     7681 cttcagaacc ggaaggaggc ataacgaaaa aggcccgcgg cgtactgcgc gcgagccctt
     7741 ctgtctgacg ttcactctca ggcggcgttc cttgccatct cgtcggcata atccttgtgg
     7801 tccacccagg tcgtgctggg cgccctcagg atcgtgtcca cgtcgccggc actcacgacc
     7861 gtatggaact catggtcgta gagccgatcg actctcactt ggcgctcgag ttcaggaacc
     7921 cagatctctg cgtcctcctt ggggagctca ttgaggagcc actccttgga caccgcgacg
     7981 cgtcgttccg gatccgcgct cgtcataatc catcgcatcc ttttctctcc cttaatgtct
     8041 gcggatgtgg aaaacgttcg ttactcacga gggcaggtag tgcatcctgt gatttcagcc
     8101 cagcccgcct cttcgctgaa tctgcccatg acgtgggctg gcacgatgaa gtggagggcg
     8161 tagtcgtacc cttccgggat cacgtcggga cgcttctcga ccggaaattc atgagcgagc
     8221 tcatgcaggc ggaacagatc cccgtccttg tactcgtcct cgagctcgat gcccttctgc
     8281 atcccggaca cccctctgcg tgggcggtcc ggaaataccc gtaaaagaac ctgcgacacc
     8341 ggaaactcct ctgctacggt atggctcaga ctagaccata gcgactgaac ggcagtaacg
     8401 caagaaccgc ccagaggggc ggtttcttat taggcgatct tgtcgatctt caccttgatc
     8461 tgcggctggc ggtgtccgcg gcgcttcaag tagttgctct tggccttgta cttgaccacc
     8521 tctaccttct tcgcgcgtcc taccgactgc acggtaccgg ttacggtctt cccggaaagg
     8581 gtcggggtgc cgagcgagag cgtgccgtta tcggtcataa ggacctggtc gaaggtgacg
     8641 gtatcacctt tcttcacgtc ctgagtagcg gcaccgcgac cgagcttctc gatcgttacg
     8701 gttgcgcccg gggagacaac gtactgcttg cctccggtct tgattactgc gagttccata
     8761 gtcccctgat agtaccgaaa taggccctaa aaggcaagag ggccttattc ctcgccgctt
     8821 ccaggcttat ccagcagctc aacctcaagt cctatcttct cctttgctat gcggtatacg
     8881 tccttttcaa ccttcccgag ctccttatac ccactgttgt aatgggagac ggcagttccg
     8941 agtgcagtgc caagcttctg gtaatacgtc tcgtacgcgg aaagatgctt cccaagctcg
     9001 cccacacgct tctggatctc ctctgcctgc ttctcaatct gaagcgcctt cagtccctga
     9061 agcacggtct gaaggtacgc aaggaaggag gtcggagaga cgatgataac cttgtacttc
     9121 cctgctgcac gctggatgag gttctcatcg tcgcccgcac ccacctggtt ggtgaggaga
     9181 tcgtagtaga tgccttctga agggatgaac atgaacgcga agtccatagt cccctcctcc
     9241 ggacggatgt acttagcggt ctccgtgatg cggttcttga ggtcagtaac gaacgccttc
     9301 tcgtacatgg ccttgtcagc gtccgctgcc tggacgtagc gattgtagtt ctcaagggag
     9361 aacttcgaat cgatcggcac gagcttgtcg tttacgtaga ctgctgcatc cacgatctcc
     9421 ccgttctcga acatgtgctg catcttgtac atgcctggcg gaagcacgtt cttgagcacg
     9481 gtctcgaggt aatactcgcc gaggacgccg cgctgcttcg ggttctttag aatgtcctga
     9541 agggacttca actgctctgc gaacttgccg gtctcacgac cgatctcctt cacactcgtg
     9601 atctcagccg tgatatcgcg gataaggcgc gaggactcgc cgagctgcga gcgcaccgtc
     9661 tcgtggatct ctcgtgagga ctcgtgcatg cgcgcgtcga gggagcgctc gagcttctcc
     9721 atctggttct ggaggagcat gagcccctga ccgtcatcct taggaacatc gttctcgcgc
     9781 cggcgaagta cgatatagag accgataccc tgtaggagca tcacgatcgc aacgatgatg
     9841 acgatggtta tggcgttcat gcaggcagta tagcggattt actctgcggc cgtatgaaca
     9901 cgagccactt agcaacctca atcgtcgcga ggttcacaag cccgaatccg acaagcatac
     9961 ctacctggag caatgtcggc ggcgatacgt gcacgatcgt ctggatagcc ggcacgtaca
    10021 aagcggcaag cagaagtccg gcgcttgcaa taagggcaag caagaggaac ttgttcgaaa
    10081 gaagcggaag cttccagagc ggagtcccga agctcttcat ggagaaggcc atgaagatgc
    10141 agttcacgga cacggccacg aacatgaggg tctggatctc agggagtgag agctcggttg
    10201 cgaagacgag gaagcagtag acggagatga gcgtcacgga tgtgatcgcg ccgatgacca
    10261 tgatgagacg tacgatcttc ggcgagatca cacgcgaggt ctccggatcc ttcggcgtgc
    10321 gcttcattgc agacggatac agcggctcga acgcgaacgc gaagttcatc aatcctccct
    10381 caaccaaatt ggaccagaga atctgcgtcg ggaggatcgg aagtggaagc ccgaatatca
    10441 aagagaacgt tatgagggca acctcgctga agttcgtcga gagcatgtac gtaaatatct
    10501 tcttgaagtt gtcccgaagc ctgcgccctt ccttgatggc gtgcgtgata acggagaatc
    10561 cgttattgag gagcacgaga tcggacgctt ctttggcaac atcggtaccc gacccaaccg
    10621 cgataccgat cgctgctgcg cgcaaggccg gcgcatcgtt cacgccgtct cctgtcatag
    10681 ccactacttc cccagcatct ttaagcacat ttgccatgcg gagcttttct gagggtgcta
    10741 cgcgcgcaaa cacggtatgg ttctgaagca cctcgagcag ctcagcgtca gaaagtcctg
    10801 aaagctcagc tcccgtatac gcacgctcat ccttatcggt tgcaataccc accgagcgcg
    10861 cgatagaaag cgccgtttcc ggattgtctc ccgtaagcat aagcacgcgg gcgcccgcgt
    10921 cctgcatgtc ccgtatcgca tccttcgcct cagctcgtat cacatccgag aacaccatga
    10981 gtcccaaaag ctcgcctttg gcaagcagtt ccggaagctc cttctcactc gggaacgcag
    11041 agcctgcgtt tgggatgcgc gctaccgcaa ttacgcgctt accgctctgt gctgccatcg
    11101 agagcgcatc ctcaaaatgg gaaagcgcct tgtctgaaag cgcgtgcgct tccccatcgg
    11161 cgtccgcaac cttgcgcatg ctcgtaagga agagctcagg cgcaccggtg acgtacgcgg
    11221 tccctcctcg gaccagcatg ccgccaaagc ggcgcgcaga atcgaaatga agctcatcaa
    11281 tgcgcggaga cacagagagg agtacaggtt gcgcaatacc attcttaaga ccagaaagca
    11341 cgatagcggc ctcgataggt cggccatgca cgacgagctt ctcgtcttcc ggaagctcgg
    11401 gccgctcttc gacgtatgca ttcgatgcaa tgactgcagc tgaaagcacc tggtgcgctt
    11461 ctcctgagaa cgcgtccgca tagtatccgg tcctgcctac gaaaagctcg agcgtcatgc
    11521 ggccttcggt aagcgtaccg gtcttgtcgg tgaggatcac gctcgtagtg ccgagcgtct
    11581 ctgcagcaag gagattacgc accaaccctc ccgaacgtag gatgcgctcc ataccgagcg
    11641 caagcaccac cgtaacggcc gccggaagac cttctggtac tgacgctacc gcaagtgcga
    11701 tagccgtgaa gatggtctcc tcgagcggaa gcccgcgcac aagaccgagc gcaatgatac
    11761 caataagcac gaaacaaacg acgataagga taaagcgcgc cacctgcctg atatcgcgca
    11821 tgagcggcgt cacggactcg tccttctttc cgagctctgc agcgatctgc ccgagctcgg
    11881 tgtcgcctcc ggttgcaacc acgatacctt tcccggctcc ggctgcgacc agcgttccgg
    11941 catacgccat accggagcgc tccactaacg gcgcgtcttc cttcgcttcg gcggtatcct
    12001 tctcaactgc aagccactct cctgaaagag ctgactcgtt cacggtgagt ccgtgtacct
    12061 ctacgaggcg caggtccgca ggcaccgcgt ttcctgttgc aagcgcta
//
LOCUS       DASWXU010000020         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_986603, whole genome shotgun sequence.
ACCESSION   DASWXU010000020 DASWXU010000000
VERSION     DASWXU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986603"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGE48_00635"
     CDS             <1..538
                     /locus_tag="VGE48_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY1041918.1"
                     /translation="LLRESRRDPSLFSELVDRYEAPFLRKARSILRNPEDAEEVVQDA
                     FTRIYLYADRYEKQEGATFSSWGYAILTRLAFTRYQKLKKHSARNLSLEPEEYERMPE
                     EGTFVEELSLKNEVLMALAKLPESASRVLRLQFLEGKTQEEIAEEEGSTVSAVKTRVH
                     RAKKLFRTALEENETPYV"
     gene            531..851
                     /locus_tag="VGE48_00640"
     CDS             531..851
                     /locus_tag="VGE48_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041919.1"
                     /translation="MSESLISHTVMRRVRAIHAMRLLAAPVTAAVVFLAALWGIGRAV
                     WVAKVFENMPTLSDVPGVLSFYLHAFLGTGILEQVLLLTASAALIAFFVSVMRNTGQE
                     MRFA"
     gene            complement(881..1945)
                     /gene="recA"
                     /locus_tag="VGE48_00645"
     CDS             complement(881..1945)
                     /gene="recA"
                     /locus_tag="VGE48_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY1041920.1"
                     /translation="MPAKKTTKKTVKKAAPEADLPEGAGGADQKQRAIDLAIKEIQTK
                     FGDEAIMAFGTGKPAAIPSIPTGSLGLDAALGIGGLPRGRIIEIFGPESSGKTTLALH
                     VIAEAQKKGGICAFVDAEHALDPEYARKIGVKLPELLISQPDTGEQALEIVESLVRAN
                     SIDVIVVDSVAALTPKDEIEGDMGQAQMGKQARLMSQALRKLTAITAKSNTIIIFINQ
                     IRMQLGVMFGNPETTTGGKALKFYTSVRLDIRRIAQIKKGEEIVGGRVRVKVVKNKVA
                     APFKTTEFDLLYNEGISREGEIIALGEKYDILQKSGSSYKYNEQTLGRGYDATRTFLR
                     ENKPVANEILKQIREKLAQD"
     gene            complement(1953..2279)
                     /locus_tag="VGE48_00650"
     CDS             complement(1953..2279)
                     /locus_tag="VGE48_00650"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041921.1"
                     /translation="MSLRLAAALVLMILVGYAGVAAWPILSGPSLSIASPQDFETLAD
                     GHAVIEGTAHHTETVWLNGAPLLMDESGSFKAALTLPSGGAVVSLTATDRFGREITER
                     RSVFVN"
     gene            complement(2283..>2832)
                     /locus_tag="VGE48_00655"
     CDS             complement(2283..>2832)
                     /locus_tag="VGE48_00655"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY1041922.1"
                     /translation="TGLIKANVPTRLALQVASQIDSRTILDASGAELLLGAGDMLYQS
                     ADMQKPVRLQTAYISEQEVKKVVSHIKQHNAGSLSSLDLGANTNNTGVHEANDAIMMA
                     AEDDDVDDDLFGAAREAVEEAGRASTSYLQRKLRIGYSRAARLMDLLEDKGVIGPADG
                     ARPREVLSRSGGGSGSDEDEDI"
BASE COUNT          536 a          794 c          869 g          633 t
ORIGIN      
        1 gctcctcagg gagagccggc gggatccaag tctcttctcg gagctcgtgg accggtacga
       61 ggccccgttc ctccggaagg cccggagcat cctcaggaac cctgaggatg ccgaggaggt
      121 ggtgcaggac gcgttcacga ggatatacct ctatgcggat cgctacgaga agcaggaggg
      181 cgccacgttc tcgtcctggg gctacgccat cctcacgcgt cttgcgttca cgaggtacca
      241 gaagctcaag aagcactcgg cccggaacct ctcgcttgag cctgaagaat acgagcggat
      301 gcccgaggag ggaaccttcg tagaggagct ttcgctcaag aacgaggtcc tcatggcgct
      361 cgcgaagctt cctgagtctg cttcccgggt actgaggctt caattccttg aagggaagac
      421 ccaggaagag atcgctgaag aggaaggaag caccgtttct gccgtaaaga cccgggtgca
      481 ccgcgcaaag aagcttttca ggaccgcttt agaagaaaac gaaacccctt atgtctgagt
      541 ctctcatttc acatacggtt atgcgccgcg tccgcgctat tcatgcgatg cggctcctcg
      601 ctgcaccggt tacagctgcg gttgtatttc tcgctgcact ctggggtatc ggcagggcag
      661 tgtgggtagc taaagtgttc gagaacatgc ctacgctctc ggacgtgccc ggagtactat
      721 ctttctatct ccatgcgttt cttggcaccg gcattctcga gcaggtactt ttgcttaccg
      781 cttcggcagc gcttatcgcg ttcttcgttt cggttatgag aaacacgggg caagagatgc
      841 gctttgccta aacaaaaggc ccgctcgagc gggccttttg ttaatcctgc gcgagcttct
      901 cgcgaatctg cttcaagatc tcgtttgcaa ccggcttgtt ctccctaagg aaggtcctgg
      961 ttgcgtcgta tcctcgtcca agggtctgct cgttgtactt gtagcttgag cctgacttct
     1021 ggaggatgtc gtacttctcg ccgagtgcga ttatttcgcc ttcacggctg atgccctcgt
     1081 tatagagaag gtcaaactcg gtggtcttga acggagcggc aaccttgttc ttcactacct
     1141 tcacgcggac cctaccgcct acgatctcct cgcccttctt gatctgggcg atacggcgga
     1201 tatcgagacg cacggatgta tagaacttaa gggccttgcc gccggtggtg gtttcaggat
     1261 tgccgaacat cacaccgagc tgcatgcgga tctggttgat gaagatgatg atcgtattgg
     1321 acttcgcggt gatggcggtg agcttacgaa gcgcctgcga catgagacgc gcctgcttac
     1381 ccatctgtgc ctggcccata tcgccttcga tctcgtcctt cggggtgagg gcagcgaccg
     1441 agtccacgac gatgacgtcg atggagttgg cacggacgag cgactcgacg atctcgagcg
     1501 cctgctcgcc ggtgtcaggc tgcgagatga ggagctctgg gagcttcaca ccgatcttgc
     1561 gcgcgtattc cggatcaagc gcgtgttccg catcgacgaa cgcgcagata ccgcccttct
     1621 tctgcgcttc tgcgatcaca tgaagcgcga gcgtggtctt gcccgaggat tccggaccga
     1681 agatctcgat gatacggccg cgggggagtc cgccgatacc aagcgcagca tcaaggccga
     1741 gggaaccggt cgggatagag ggaatcgccg caggctttcc ggtgccgaag gccatgatgg
     1801 cctcgtctcc aaacttcgtc tgaatctcct taatcgcaag gtcgattgcg cgctgctttt
     1861 ggtctgcccc gcctgcgccc tccgggagat cagcctcagg agctgctttc ttcacggtct
     1921 tcttggtggt tttctttgca ggcatacaag tattagttaa cgaatacgga acgacgttca
     1981 gttatctcgc gtccgaaccg gtctgtcgcg gttaaggata ctacagcgcc ccctgaaggg
     2041 agggtgagtg ctgctttgaa ggagccgctc tcgtccataa gaagcggtgc gccgttgagc
     2101 cagacggtct ccgtatggtg tgcggtgcct tcgataacgg catggccgtc agcgagggtc
     2161 tcgaagtctt gaggactcgc tatcgagagg gagggacctg aaaggatagg ccaggctgcg
     2221 acgccggcgt acccgacgag gatcatgagc acgagcgcgg cagcaagacg tagtgacata
     2281 ggctagatat cctcgtcctc gtcgcttccg ctgcctccgc ctgaacggga gaggacttcg
     2341 cgtggacggg cgccgtctgc aggcccgatg acacccttgt cctcaaggag gtccatgagg
     2401 cgagcggcgc gggagtagcc gatacggagc tttcgctgaa ggtatgaagt cgatgcacgt
     2461 cctgcttcct cgacggcctc acgggcagct ccgaagaggt cgtcatccac gtcatcgtcc
     2521 tcggcagcca tcatgatggc gtcgttcgcc tcatgaacac cggtgttgtt tgtgttcgct
     2581 ccgagatcaa gggaagagag ggaaccggcg ttgtgctgct tgatgtggga caccaccttc
     2641 ttcacttcct gctcgctgat gtaggcggtc tggaggcgca ccggcttttg catgtcagca
     2701 gactggtaca gcatgtctcc ggcgccaagg aggagctctg cgcctgaggc gtcgaggatg
     2761 gtgcgcgagt cgatctggct cgctacctgg agcgcgaggc gggtagggac gttcgccttg
     2821 atgaggccgg ta
//
LOCUS       DASWXU010000021         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_884028, whole genome shotgun sequence.
ACCESSION   DASWXU010000021 DASWXU010000000
VERSION     DASWXU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884028"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..660
                     /locus_tag="VGE48_00660"
     CDS             85..660
                     /locus_tag="VGE48_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY1041923.1"
                     /translation="MQSSSFHSVLLTGRPGSGKGTQAKRLAAEFGWTHFSTGERFKQL
                     RDGTGPIAAHVKEAYDAGELLPDWFANYLIEEVALSLPEGQGLVCDGYPRTRQQAERF
                     HAVMEWLERPYIVLDLAVEEEEAISRMKSRALVEDRPDSGTDAQVRARLKVYENYTAP
                     MLDFFREKGVFTELDGMQTPDEVAAAIRASL"
     gene            663..1442
                     /gene="map"
                     /locus_tag="VGE48_00665"
     CDS             663..1442
                     /gene="map"
                     /locus_tag="VGE48_00665"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY1041924.1"
                     /translation="MIVRTLEEREGIIESGRRLGVILGKIEAMVAPGVSTQELEDEAV
                     RLIEEGGDKPAFKGYTPEGAPRPFPAALCVSVNEEVVHGIPNENPRILKEGDIVSLDF
                     GIVHQGYFTDSAVTVPVGKVSKEAAALIAATEYALEEGIKAAKVGGRIGDISAAIGAT
                     FKSSPFSIVKVLGGHAVGRKVHEEPYIANYGHAGTGEELKPGMVLALEPIANAGKGTV
                     IIAPDGHTYRSKDGSISSHSEHTILIEDEGTIVVTRRPSEK"
     gene            1512..2123
                     /locus_tag="VGE48_00670"
     CDS             1512..2123
                     /locus_tag="VGE48_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY1041925.1"
                     /translation="MSNPHPSTERTLVIVKPDGVQRGLVGEVIKRYEQVGLKLVGIKI
                     GVADAEKIEQHYTLDPEWRRVTGEKTIESYQKKGLTPPSEDPLEITAVILQNLKSYMT
                     SGPVVFMVWQGAHAVKIIRKITGGTEPLTSDVGTIRGDYVLDSYQMADLDSRAVRNVI
                     HASGSPEEAEMEIKHWFLPSEIIDYRLVNEQIIYDANLDGILD"
     gene            2184..2678
                     /locus_tag="VGE48_00675"
     CDS             2184..2678
                     /locus_tag="VGE48_00675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1041926.1"
                     /translation="MTDTEPLYIDQGPDAPRPDLPFIERDAVVALVRDPQARKYLGLK
                     WKEVDWETFVTGGIEEGQTAEEAARAEILEETGYKNLRLVADLPRYHTKFYHPPKGVN
                     RFAHFQCFLFELENDDRNPVNEEEAGKHEAIWLTEEDLASFRLTEAQQVLLTHAKGIA
                     WGGE"
BASE COUNT          646 a          786 c          880 g          606 t
ORIGIN      
        1 cgaggttgca ggcgaggtcg cacgtgtcct tggcatcgcg tagcggtgcg cgccagcagc
       61 tttattgata cagtagcgat acatatgcaa tcatcatctt ttcatagcgt gcttcttacc
      121 ggccgccctg gttcagggaa aggtacccag gcaaagcgcc ttgcggccga attcggctgg
      181 acccacttct cgaccggcga gcgcttcaag cagctgcgcg acggtaccgg ccctatcgcg
      241 gcccatgtga aggaggcgta cgacgcgggt gagctccttc cggactggtt cgcgaactac
      301 ctgattgagg aagtagcgct ctcgctgcct gagggtcagg gtctcgtatg cgacggatac
      361 ccgcgcaccc gtcagcaggc ggagcggttc cacgcggtta tggagtggct cgagcgtccg
      421 tacatcgtgc ttgaccttgc cgttgaggaa gaggaagcta tttctcgcat gaagagccgc
      481 gcgctcgtag aggatcgtcc ggatagcggc actgacgctc aggtgcgcgc tcgtctcaag
      541 gtgtacgaga actacaccgc gcccatgctc gacttcttcc gggagaaggg cgtgttcacg
      601 gagctcgacg gtatgcagac cccggacgag gttgccgcgg caatccgtgc ttcactatag
      661 ccatgatcgt acgcacgctt gaggaacgcg agggtatcat cgagtctggt cgcaggctcg
      721 gcgtgatcct cgggaagatc gaggcgatgg tggcgcctgg agtgtctacg caggaactcg
      781 aggacgaggc ggtgcgtctt atcgaggaag gcggcgacaa gcctgcattc aagggctata
      841 cgccagaagg cgcaccgcgc ccattcccgg cggcactctg cgtctcggtg aacgaggaag
      901 tggtccacgg catcccgaac gagaacccgc gcattctcaa ggaaggcgat atcgtatcgc
      961 tcgatttcgg catcgtgcac caggggtact tcacagattc tgcggttacg gtaccggttg
     1021 gaaaggtatc aaaggaagct gctgcgctca tcgcggctac tgagtacgca cttgaggagg
     1081 ggatcaaggc ggcaaaggtt ggcggacgta tcggcgatat ctcggcagcc attggtgcta
     1141 ccttcaagag ttcgccgttt tccatcgtga aggtgcttgg cgggcacgcg gtggggcgca
     1201 aggtgcatga ggagccgtac atcgcgaact acggtcacgc aggcaccgga gaggagctca
     1261 agcctggcat ggtgctcgcg cttgagccga ttgcaaacgc aggaaagggt acggttatca
     1321 tcgcgccgga cggacatacg taccgctcga aggacggttc aatttcctcg cactcagaac
     1381 atacgatcct catcgaagat gagggtacga ttgtggtcac gaggcgtcca agtgagaaat
     1441 agacctcggg cttagaactt gctatagtct gcgtatacag gcgccccagg gcgattttta
     1501 gtatcaaatc catgtctaat ccgcatccaa gcaccgagcg tacgctcgtg atcgtgaagc
     1561 cagacggcgt tcagcgcggt ctcgtaggtg aggttatcaa gcgttacgag caggtgggtc
     1621 ttaagctcgt cggcattaag atcggtgttg cagacgcaga aaagattgag cagcactaca
     1681 ccctcgatcc ggagtggcgc cgcgttactg gcgagaagac catcgagagc taccagaaga
     1741 agggtctcac tcctccatct gaggacccgc ttgagatcac ggccgtaatc cttcaaaatc
     1801 tgaagtccta catgacttca ggtcctgttg tgttcatggt gtggcagggc gcgcacgcgg
     1861 ttaagatcat ccgcaagatc acgggtggta ctgagccgct tacgtcagac gtgggtacga
     1921 tccgcggcga ttacgtgctc gactcgtacc agatggcaga cctcgatagt cgcgcagttc
     1981 gcaacgtgat tcacgcgtcc ggctctcctg aagaggccga gatggagatc aagcactggt
     2041 tcctcccgag cgagatcatc gactaccgcc tcgtaaacga gcagatcatc tacgacgcta
     2101 acctcgacgg cattctcgac taggttctca tccgaatagt gcgcctggta tatcccctcg
     2161 agagagggga tatactgttt tatatgactg ataccgaacc tctatacatc gaccagggtc
     2221 cagatgcgcc tcgtcctgac cttccgttta tcgagcgtga tgcagttgtc gcgctggtgc
     2281 gagaccctca agcccggaaa tatctcggac taaagtggaa ggaggtagat tgggagacat
     2341 ttgtcacagg gggcatagaa gaaggacaga ccgctgaaga ggccgctcgt gcggagattt
     2401 tggaagagac gggatacaag aatttgaggc tcgtagctga tttacctcgc taccacacaa
     2461 agttctatca ccccccgaag ggcgtgaacc gcttcgcgca cttccagtgc ttcttgttcg
     2521 aactcgagaa tgacgatcgg aatccagtaa atgaggaaga agctggaaag cacgaagcaa
     2581 tctggcttac cgaagaagac cttgcctcat tccgccttac ggaagctcag caggtgctcc
     2641 ttacgcacgc gaaaggcatc gcttggggtg gagaatagga aaagggtata ctttgtttag
     2701 agccgtccca aaacgcaact tgctcaattt cagggagcct tatatcccgc gtttccgcaa
     2761 agccctcggg ctttcaagga cgcgcgatgc gggcacccac gtggcatcca aaagttgcca
     2821 ctagggacgg ttcttccgag aaacccgaga cgaaggtctc gggttttcgc gtgctagagt
     2881 gtagccatgc gccttttctg gctccttgct gcgctcgt
//
LOCUS       DASWXU010000022        11928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_15674, whole genome shotgun sequence.
ACCESSION   DASWXU010000022 DASWXU010000000
VERSION     DASWXU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15674"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VGE48_00680"
     CDS             complement(<1..819)
                     /locus_tag="VGE48_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041927.1"
                     /translation="MGLFSFKKSKGTSIAVIDINSASVGGAYVRYEEGTLPVIYYTAR
                     VDVEPRESEPILDSMLRSLSFLEQLMVLEGAPALRRETGDGSVERVLVSVSAPWQETE
                     VATTHLEEKKPFTFTHAHLDAVGNAPLKKDRIRSGRTVIATVLNGYETPNPFGKRVTR
                     ADLVVLSSSLEKEAARQIEASIRKAFHTGNIELVAFSPASYTVFRDLYPHQKDFLLLD
                     VSGSGTDVALVKRGMLVETRTVSYGTHDLERAARKAGQRAHLGADQMLDPEANKT"
     gene            complement(868..2784)
                     /locus_tag="VGE48_00685"
     CDS             complement(868..2784)
                     /locus_tag="VGE48_00685"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY1041928.1"
                     /translation="MKLSDVRSRFLAFYEKRGHAIIPSAPIVPGNDPTTLFTSSGMQP
                     LVPYLLGEVHPAGTRIVDSQLSFRAGDIEEVGDNRHTTFFEMLGNWSLGDYFKEEQLP
                     WVFEFLTSKEEGLGLDPERLYVTVFAGDESTGMGPDMEAVSIWQRLYKEKGIDARFAN
                     IGSEADGYQKGMQGARIFAYDAKKNWWSRAGVPANMPAGEPGGPDSEIFYDFGLPHDT
                     SWGPECHPNCDCGRFVEIGNSVFMQFVKAEDGSFVPLPKQNVDFGGGLERLGMASIGT
                     PDAFLLDVFDAARGVLEARTGKAYGSDEGTTRAYRIILDHMRAASFMLAEDIKPSNTE
                     AGYVLRRLIRRAIREADRLGIKDAVLADVAKGYGEAYADHYPHVLQHADMIREELAKE
                     EEKFRKTLAQGMRELEKLVAKDLLTPDAAYTLFTTYGFPVELTEEIAKERGIVLDMEA
                     VAAKMKEHQNQSRAGAAQKFAGGLADHSEQTVRYHTTHHILLKALQMVLGMEVKQRGS
                     NITAERLRIDFASPGKMTDEQKAEVERIVNDVIYQDLPVTRTVMPREEAEKLGAEMEF
                     GVKYPDMVSVYSVGPAHAVEGNPDIPNAFSLEFCGGPHVTNTREIHEGGMKFRIQKEE
                     AVAAGVRRIKGVLA"
     gene            complement(2796..4841)
                     /locus_tag="VGE48_00690"
     CDS             complement(2796..4841)
                     /locus_tag="VGE48_00690"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY1041929.1"
                     /translation="MNADTILRGDEGMWPLNMIPKDRLKKLHKVSVDDAWIEDVQRAA
                     VRMNNGGSASFVSKDGLIATNHHVAADVLYNLSSKEKDYMKDGFLAKTRAEELKAKQL
                     EVNVLWEIEDVTKQIEESLKGITDLEKRMEKRRAVIASIEKESKDRTGMRSDVVTLYN
                     GGQYHLYRYKKYTDIRLVFAPESAIASFGGDIDNYEYPRYSLDVTFFRVYENGKPLQS
                     PSYFSWSTRAPKLGEPLFVAGHPGSTDRLSTYDRIVLMRNESMPRLMDYVRRQEVLLQ
                     QFAGRSPEHARRADDRLHSIQNVRKRYAGQLASIQTQSFTDALEKREQEIRAAIKKNP
                     AIAKRTGKAWDEIKEALELFRAHRDEYLMFEAGRGFRSELFGIAHTIVRLVEEDTKPN
                     AKRLEEFGDARRDSLLEELYAPTPIYKDLEEHLFADALRYLVETLGFEDKDVKRILGG
                     RSVEVVAKEYVGKSKLDSVAERKKLVKGGKAAVAASKDPFIQLALSIDPRARKVRKLV
                     ETRVDTVMEDAYGRIAQALFSLYGESIYPDATFTLRLTYGKSVPYAVEKVPEPNYTTI
                     GGVFAHAALHKEEFPWKLPASWEKQRAKLVQDKSAFNFITTHDTHGGNSGSPVFTPNK
                     EIVGLLFDGLVHTQGGDSFMYMADEKEHTVCVHSQGIYSVLKTVYKADALARELSGK"
     gene            complement(4869..5534)
                     /locus_tag="VGE48_00695"
     CDS             complement(4869..5534)
                     /locus_tag="VGE48_00695"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY1041930.1"
                     /translation="MEVRMEPSWKEKLAGEFKKPYFAELTKFVKEEYQNGAVYPHPKH
                     IFRAFELTPFDQVKVVILGQDPYHGPGQAIGLSFAVEEGTTLPPSLRNIYKELADDLG
                     TPIDAYGDLSRWAKQGVLLLNATLTVRARTAGSHQGKGWEAFTDAAIKALSEEREHLV
                     FILWGNYARSKGAHIDRAKHLVIESAHPSPFSASNGFFGSKPFSKANAYLMEHGKEPV
                     DWS"
     gene            complement(5538..6320)
                     /locus_tag="VGE48_00700"
     CDS             complement(5538..6320)
                     /locus_tag="VGE48_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY1041931.1"
                     /translation="MADWKRYNPNRSADWNYGGKKWRLSRSKIEFFLECERCFYLDNK
                     LGTKRPGYPEFTLNKAVDQLFKKEFDGHRKAGTRHPLMEQYGIKAVPFSHANIDTWRE
                     NFEGVEYKDPETGFVISGAVDDIWVDENGQLIVVDYKSTSKPGKIETLGDSSWNEQYR
                     RQMGVYQWLLRKNGFDVSDMGYLVYANADGDKEAFDGKLEFEVTVVECEGQTGWIDEV
                     LPRIKAVLDVEAIPPIGDACEFCPYREASGKKLLALHQKNKK"
     gene            complement(6323..7159)
                     /locus_tag="VGE48_00705"
     CDS             complement(6323..7159)
                     /locus_tag="VGE48_00705"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEY1041932.1"
                     /translation="MKPEIIKADGTIEIFDGVKLESSLRRAGASDVTAERIRHTIEAT
                     MGPRAESSDIYRRAFQMLRTDARPAAARYSLRRALFDLGPTGHPFEDFVAELFKKEGW
                     TVEGRRMIPGKCVMHEVDVYAKRGNEHLAAELKYHNSPGYKTDVKVALYVKARFEDIW
                     QCKEEDQKTCPVDIGFLITNTKFTKDAIDFATCSGMQLLGWSYPHGSSLYDRIVASGL
                     FPVTALTTLKKSEKRLLIDQGIVTCQQVREHRDLLRGIGLTPERIGTVVAETNTLCGA
                     NA"
     gene            complement(7160..8179)
                     /gene="mnmA"
                     /locus_tag="VGE48_00710"
     CDS             complement(7160..8179)
                     /gene="mnmA"
                     /locus_tag="VGE48_00710"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY1041933.1"
                     /translation="MSLKGKRVFVGLSGGVDSAVTAALLKKGGATVAGIFIKGWYPPG
                     MPCTWAEDRRDAMRVAARLQIPFLTLDASKEYKQGVIDYLLAEYKAGRTPNPDIMCNR
                     EVKFGAFYRFTKEKGADHIATGHYARVENGRLLRGVDSAKDQSYFLWAVPEEALSMTL
                     MPLGSMKKDETRTLAKRFDLPNARKKDSQGICFLGSISVEDFLRQELGQQEAGIARDT
                     EGNELGLHDGALFHTLGSRIAIEGASQGPWYVVRKDVEKNELVVSKDRVSPPDRIELS
                     QTNWFETPEGEVEAQFRYHGPVIKGTLSGNTFVPNEALPEPAAAGQSLVIYRNDRVIG
                     GGIIG"
     gene            complement(8198..9574)
                     /locus_tag="VGE48_00715"
     CDS             complement(8198..9574)
                     /locus_tag="VGE48_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041934.1"
                     /translation="MSAGGHGGGGLPGLSAIQHWMHKAHEDGGSSVELKPLLIAFCAE
                     LVAFFFFSPSQTITNFQFLLFLSPLWIPALLLHFGLRRFIDANKTAWIAKQETVLLEI
                     KMPRDTRRTPLAMETVFSSMHIAGGENTWYKRIIQGRARPWYSYEIVSIGGRVHYFVW
                     LRTGWRRALESYLYAQYPGVEVIEAMDYSRLVDPSHEPYTMTAFEFRKTKPDPYPLKT
                     YVEYGLDQVQKPEEQIDPLAQMIEIMGSLGPKEQLWLQFVVRVHQGKPWDSKPASWKD
                     SAKEEIKKLREEDKVENEYVDANGVKRTSISYPNPTRAMATRIEAIEKNTGKQAFDVG
                     IRGIYLAPKDVAQGTMGGVLVNIFKPFNSENANSLMPADGRWSNHYNDYPWEDPHGHH
                     TEHMNHMVLLYYRMRAFFHPPYVGDYNIMSVEEMATLYHVPSSSVTTPSLPRIQSTTS
                     EAPSNLPT"
     gene            complement(9644..10753)
                     /locus_tag="VGE48_00720"
     CDS             complement(9644..10753)
                     /locus_tag="VGE48_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY1041935.1"
                     /translation="MAKSAKKPAAASKKVPAAKKAKAPKAASASLSFDTVALVKKLLS
                     EVPERGREVLTLRFGLGTAQRRETLEAIGERWSITRERVRQIEAAGIESIRNSKAFKE
                     SQEAFDELASHIEGQGGIVPEDVLLAGLASDEKAKNRFRFLLVVGSAFFRERETDDFY
                     ARWHVDHKTAEAVHTALSRLYETLDDAEVLSEGDIINRFLEELKGVNEAYQNEEILKR
                     WLTISKTIAKNPLSEWGKAHAPAIRTKGIRDYAYLVIKQKGEPMHFADVASSIVEVFN
                     KKAHVATTHNELIKDSRFVLVGRGLYALAEWGYQPGVVRDVIREVLEKHGPLTRDAII
                     EEVKKVRYVKDNTILVNLGDTRHFKRQKDGTYQVA"
     gene            complement(10802..>11928)
                     /locus_tag="VGE48_00725"
     CDS             complement(10802..>11928)
                     /locus_tag="VGE48_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1041936.1"
                     /translation="YWGCPIPVVYDPEGNPHAIPAEHLPWLLPTDVDFTPSGKSPLAT
                     SKELQERVTRIFGEGWTPEYDTLDTFVDSSWYFLRYLDSKDEHDFSDQSVMKRWMPVN
                     RYSGGSEHTTMHLLYSRFFVKALYDLALVPVAEPFVERFNRGLILGPDGAKMSKSKGN
                     VINPDSVVKELGADAVRMYLAFIGPYNEPGTYPWNLDGAAAMRKFLDRIYALKEKVTE
                     GEAAPAEAKALAQATTKVSEDIERFKYNTAISALMVLVRDLEKQEQVSKEAYGELLQL
                     LAPLAPHLADHLWQELGNEGSVHQASWPTREVPEDDTMTIVVQVNGKKRGEVVLAKTA
                     SEEEVIAAARGLQFVREALTIGAISRAVYVPGRILSLVIG"
BASE COUNT         2465 a         3515 c         3162 g         2786 t
ORIGIN      
        1 cgtcttgttc gcctctggat cgagcatctg gtcagcgcct agatgcgctc gctgtccagc
       61 tttgcgggcg gcacgctcaa ggtcatgggt gccgtagcta accgtacggg tctcaacgag
      121 catgccacgc ttaacgagag ccacgtccgt accggatcct gagacatcga gcagtagaaa
      181 gtccttctgg tgcgggtaca aatcacggaa caccgtatag gaagcaggcg agaacgcgac
      241 aagctcgatg tttccggtat gaaatgcctt acgaatggag gcttcaatct gtcgagccgc
      301 ttctttctca agcgaagaag agagcacgac cagatcagcc ctggttacgc gctttccgaa
      361 cggattcggc gtctcgtatc cgttcaggac ggttgctata acggtcctac ctgagcgaat
      421 gcggtccttt tttaaaggcg cgttccctac cgcgtccaag tgcgcatgcg tgaaggtgaa
      481 cggcttcttc tcctcaagat gcgtggttgc cacctcagtt tcctgccaag gagcggacac
      541 cgatacaagc accctttcta cggacccgtc tcctgtctcg cgcctgagtg ccggcgcgcc
      601 ctcaagtacc atgagctgct caagaaacga gagggaacgc agcatgctgt cgaggatcgg
      661 ctcgctttcc cgtggctcca catcaacgcg tgcggtgtag tagataacag gaagcgttcc
      721 ttcctcgtat cgcacatatg ctccgcctac tgatgcggag ttgatgtcga ttacggctat
      781 ggaagtgccc ttactcttct tgaacgagaa aagacccatg cgcgaagtat agcgcatggg
      841 tcttttcgtt tcgctgtgtt aatgcaccta tgcaagaact ccctttatgc gacgcactcc
      901 cgcagcaacc gcctcctcct tctgaatgcg gaacttcatt ccgccttcgt gtatttcgcg
      961 cgtattcgtg acatgcgggc cgccgcagaa ctcgagcgag aacgcattcg ggatatcagg
     1021 attcccttca accgcatgcg caggacccac tgaatacacg gacaccatgt ctgggtactt
     1081 tacgccaaac tccatttctg ctccgagctt ctcggcttct tcgcgcggca taacggtcct
     1141 cgtaaccgga aggtcctgat agataacgtc gttaacgatc ctctccactt ccgctttctg
     1201 ctcgtctgtc atcttccctg gagacgcgaa gtcgatacgc aggcgctcag ccgtaatatt
     1261 tgagccgcgc tgcttcacct ccatgccaag caccatctgc agcgccttaa gaaggatgtg
     1321 atgcgtggtg tggtagcgaa cggtctgctc cgaatggtcc gcaaggccgc ctgcgaactt
     1381 ctgagctgcg cctgcacgag actggttctg atgctctttc atctttgcag caaccgcctc
     1441 catatcaagc acgataccgc gctctttagc gatctcttct gtgagctcga cagggaagcc
     1501 gtaggtagtg aaaagggtgt acgcagcgtc aggcgtgagt aggtccttcg ctacgagctt
     1561 ctcgagctcg cgcatgccct gcgcaagcgt cttcctaaac ttctcttcct ccttcgcgag
     1621 ttcctcgcga atcatgtccg catgctgaag gacatgcggg tagtgatcgg catacgcttc
     1681 gccgtatccc ttcgcaacgt cggcaaggac ggcatccttg atgccgagcc ggtccgcttc
     1741 gcggatagcg cggcggataa ggcgacggag cacgtaccct gcctccgtgt ttgacggctt
     1801 aatgtcttcc gcaagcatga aggatgctgc gcgcatgtga tcaagaatga tgcggtacgc
     1861 gcgcgttgtg ccctcgtctg agccgtacgc ttttccagta cgtgcttcaa gcacgccgcg
     1921 cgcagcatca aacacgtcga gcaagaatgc gtccggggtg cctatagacg ccatgccaag
     1981 acgctcgaga ccgccaccga aatccacgtt ctgcttcgga agcggcacga agctcccgtc
     2041 ctcagcctta acaaactgca tgaacaccga gttcccaatc tcgacgaacc gaccgcaatc
     2101 gcagttagga tggcactcag ggccccatga ggtgtcgtgc gggaggccga aatcgtagaa
     2161 gatctccgaa tctggtccgc ctggctcgcc tgcaggcatg ttcgcaggaa cccccgcgcg
     2221 gctccaccag ttcttcttcg cgtcgtacgc gaagatgcgc gcgccttgca tgcccttctg
     2281 gtatccgtcc gcttcagaac cgatgttcgc gaaccttgca tcgattccct tctccttgta
     2341 gagacgttgc cagatggata cggcctccat atcaggaccc atgccggtac tctcgtcccc
     2401 agcaaacacc gttacataaa gtctttccgg atcaagcccg agaccttctt cctttgaggt
     2461 tagaaattcg aatacccacg gaagctgctc ttctttgaaa tagtctccga gagaccagtt
     2521 gccgagcatc tcgaagaacg tggtgtggcg gttgtcgcca acctcttcga tgtctccggc
     2581 acgaaaggag agctgcgagt ccacgatacg agtgcctgcc gggtgcactt ctccaagaag
     2641 gtacggcacc aagggctgca ttcccgaaga ggtgaagagg gtggtcggat cgttcccggg
     2701 cacgatcggt gccgaaggga tgatggcgtg cccgcgcttc tcgtagaacg cgaggaaccg
     2761 gctgcgtacg tcgctaagct tcatatacgg ctactttact tgcccgaaag ctcgcgcgct
     2821 agggcatcgg ccttatacac agtcttcaga accgaataga taccctgcga atgtacgcag
     2881 accgtatgtt ccttctcgtc cgccatatac atgaacgaat ctcctccctg cgtatgaaca
     2941 agcccgtcaa aaagaagccc tacgatctcc ttattcggcg taaataccgg actgcctgag
     3001 tttccaccgt gtgtgtcgtg cgtggtaatg aagttgaatg cggacttgtc ttgtacaagc
     3061 ttcgcgcgct gcttctccca ggacgcaggc agcttccatg ggaattcttc cttgtgaagc
     3121 gctgcatgtg cgaacacacc gccaatcgtg gtgtagttcg gttcaggaac cttctcaacc
     3181 gcatacggca cgctcttgcc gtacgtaagg cggagcgtaa aggtcgcatc aggatagatg
     3241 gactctccgt acaggctgaa caaggcttgc gcgattcgtc cgtacgcgtc ttccataact
     3301 gtatcgacgc gtgtctcgac gagcttacgc accttgcgcg cacgcggatc gatagagagc
     3361 gcaagctgga tgaaaggatc cttcgatgcc gctaccgcgg ccttcccacc tttcacgagc
     3421 ttctttcgct cagccaccga atcaagcttt gacttcccta catattcttt ggcaaccact
     3481 tcaacagagc gtcctcccag gatgcgcttc acatccttgt cctcaaagcc aagcgtctcg
     3541 acgaggtatc gaagcgcatc agcaaacaag tgctcttcaa gatccttata tatgggcgtc
     3601 ggcgcgtaca actcctcgag aagcgagtcc cttcgtgcgt cgccgaactc ctcaagtcgc
     3661 ttcgcgttcg gcttcgtatc ctcttcaacg aggcgaacga tggtatgcgc gatgccgaac
     3721 aattccgaac ggaatccgcg tcctgcctcg aacatgaggt attcgtcgcg atgtgcacgg
     3781 aagagctcga gtgcttcctt gatctcgtcc caggccttgc cagtacgctt tgcgatcgcc
     3841 ggattcttct taattgcagc gcgaatctct tgttcgcgct tttcgagcgc gtccgtgaag
     3901 ctctgggtct ggatggaggc gagctgccct gcgtaccgct tccggacgtt ctggatggaa
     3961 tgcaggcgat catcagcgcg acgcgcatgc tccggggacc tgcctgcaaa ttgctgcaag
     4021 agcacctcct ggcgacgaac gtaatccatg aggcgcggca tagactcgtt acgcatgagc
     4081 acgatgcggt cataggtgga aaggcgatcc gtgctgcctg ggtgtcctgc aacgaacaaa
     4141 ggctcgccaa gcttcggcgc gcgtgtagac catgaaaaat aactcggaga ctggagcggc
     4201 ttgccattct cgtacacgcg gaagaatgtc acgtcgagag agtagcgagg atattcgtag
     4261 ttatcgatgt cgccaccgaa cgaggcgata gcactttcag gggcgaacac gagacggata
     4321 tcagtgtact tcttataacg gtacaggtgg tactgtccgc cgttatacag cgtcaccacg
     4381 tcgcttctca tgccggtgcg atccttcgat tccttctcga tagaagcgat cacggcgcga
     4441 cgcttctcca tgcgcttctc aaggtccgta atccccttca gagactcctc tatctgcttc
     4501 gtaacgtcct cgatctccca caacacattc acctcaagct gcttcgcttt gagttcttcc
     4561 gctcgtgtct ttgcgagaaa cccatccttc atgtagtcct tctctttcga gctgaggttg
     4621 tagagaacat cagcagcgac gtgatgattg gtcgcaataa gaccgtcttt tgagacgaac
     4681 gaggcagatc cgccgttatt catgcgcact gccgcgcgct gaacatcctc gatccacgcg
     4741 tcgtctaccg ataccttgtg aagcttctta agacgatcct ttgggatcat gtttaggggc
     4801 cacatgccct catcccctct gagtatggtg tcagcattca tagtacaagg actgtaccac
     4861 acgcccgatt aggaccaatc tacaggctct tttccgtgct ccatgaggta cgcattcgcc
     4921 ttgctaaatg gcttcgaacc aaagaagccg ttggatgctg agaacgggga cgggtgggca
     4981 gactcgataa ccagatgctt ggcgcggtct atgtgcgctc ctttgctgcg cgcatagttg
     5041 ccccagagta tgaaaacgag atgctcgcgc tcttctgaaa gcgccttgat ggccgcgtcc
     5101 gtaaacgctt cccagccctt tccctggtgc gagcctgcgg tgcgggcacg taccgtaagc
     5161 gtggcgttga gaagaagtac accctgcttc gcccaacgag acagatcgcc atacgcgtct
     5221 attggtgttc cgagatcgtc cgcaagctcc ttatagatgt tccgcaggga cggcgggagt
     5281 gtcgtacctt cttctaccgc aaacgagagg ccgatggcct gtccaggtcc gtgatacggg
     5341 tcctgtccga gtatcaccac cttcacctga tcaaatggcg ttagctcaaa cgcgcggaag
     5401 atatgcttcg ggtgcgggta caccgcgcca ttctgatatt cctctttcac gaacttcgta
     5461 agctccgcga aatatggttt tttaaattcc cccgcaagtt tctctttcca agatggctcc
     5521 atgcgcacct ccatacgcta tttcttattc ttctgatgga gcgccagcag cttctttcca
     5581 gacgcttcac gatacgggca gaattcgcac gcgtcgccga taggcggaat agcctccacg
     5641 tcgagtacag ccttaatcct cgggagcact tcgtcaatcc aaccagtctg tccttcgcac
     5701 tcgacaactg ttacctcgaa ctcgagcttc ccgtcgaacg cttccttgtc cccgtccgcg
     5761 ttcgcatata cgaggtatcc catatccgaa acatcgaacc cgttcttccg aaggagccac
     5821 tggtacacgc ccatctggcg acggtactgt tcgttccagc tcgagtctcc cagggtctca
     5881 atctttcctg gcttagaggt ggatttgtaa tcaacgacaa tgagctgtcc gttctcatct
     5941 acccagatat cgtctacggc gcctgaaata acgaatccgg tctccgggtc cttgtactcg
     6001 acgccttcga agttctcgcg ccaggtatct atgttggcgt gactgaacgg caccgccttg
     6061 atgccatatt gttccatcaa aggatgccgc gtacctgctt ttcggtgtcc gtcgaactct
     6121 ttcttgaaga gttggtctac ggccttgttg agcgtgaact ctgggtaccc ggggcgcttg
     6181 gtgccaagct tgttatcaag atagaagcag cgctcgcact cgaggaagaa ctcgatcttg
     6241 gagcgggaga ggcgccattt cttgcccccg tagttccagt ctgcgcttcg gtttgggttg
     6301 tatcgcttcc agtccgccat acctatgcgt tcgctccgca aagcgtgttc gtctcagcaa
     6361 caaccgtacc gatgcgctcg ggcgtgaggc cgatgccgcg caaaaggtcc cggtgctcgc
     6421 gcacctgttg gcaggtgaca ataccctgat ctatcaaaag acgcttttcg ctcttcttaa
     6481 gcgtcgtaag cgcagttaca gggaagagtc ctgacgccac gatgcgatcg tagaggctcg
     6541 aaccgtgcgg atagctccac ccgagaagct gcatgcctga gcaggtagcg aaatctatcg
     6601 cgtccttcgt gaacttggtg ttcgtaatca agaacccgat atcaaccgga caggtcttct
     6661 ggtcctcttc tttgcactgc caaatatctt cgaagcgcgc cttcacgtag agcgccacct
     6721 tcacatcggt cttatagccc gggctgttgt ggtacttgag ctcagccgca aggtgctcgt
     6781 tcccgcgctt cgcgtacaca tccacctcat gcatcacaca cttcccaggg atcatgcgac
     6841 gaccctccac ggtccaaccc tctttcttga agagctccgc cacgaagtcc tcaaacggat
     6901 ggcctgtagg accaaggtca aagagcgcac gacgcaagct gtaccgagcg gccgcaggac
     6961 gcgcgtccgt acgcagcatc tggaaggcac ggcgatagat atctgagctc tctgcacgag
     7021 gccccatagt ggcttcgatc gtatgacgaa tgcgctctgc cgttacatct gacgcgcccg
     7081 cgcgacgcag cgagctctcg agtttcactc cgtcgaagat ctcgatggtt ccgtctgcct
     7141 taatgatctc gggcttcatt taccctatga taccaccccc aataactcgg tcgtttctgt
     7201 agataacgag cgactggccc gctgctgcag gctcaggaag tgcttcatta ggaacgaacg
     7261 tatttcctga aagcgtgcct ttgataaccg gtccgtggta ccggaactgc gcttcaactt
     7321 ctccctcagg agtttcaaac cagttcgtct gtgagagctc gatacgatct ggcggcgata
     7381 ccctatcctt cgatacgact agttcgttct tctcgacatc cttcctaacc acgtaccacg
     7441 gaccctgcga agcgccctct atcgcaatcc gactcccgag tgtatggaag agcgcgccat
     7501 catgtagccc aagttcgttg ccttcagtat cgcgcgcgat tcctgcctcc tgttgtccaa
     7561 gctcctgccg gaggaaatcc tctacggaaa tgcttccgag gaagcagatt ccttggctgt
     7621 ccttcttgcg ggcgtttgga agatcgaaac gtttcgcaag cgtgcgagtc tcatccttct
     7681 tcatgctccc gagcggcata agcgtcatgg aaagcgcctc ttcgggcact gcccagagga
     7741 aatagctctg atccttggca ctatctacgc ctcgaagcag tcttccgttc tcaacgcgcg
     7801 catagtgtcc ggttgcgata tggtccgcgc ctttttcctt cgtgaagcga tagaaagcgc
     7861 cgaactttac ctcccggtta cacatgatgt ccgggttcgg cgttcttccc gccttatatt
     7921 cagcaagaag atagtcgatt acaccctgct tgtattcttt ggaggcgtcg agtgttaaga
     7981 agggaatttg cagccgtgct gcaacacgca tcgcatccct gcgatcttct gcccaggtgc
     8041 agggcatgcc gggcggatac cagcccttta tgaatatgcc cgcgacagta gcacctcctt
     8101 tcttaaggag cgccgcggtt acggcagaat caacgccgcc agataagccg acaaaaaccc
     8161 ttttaccctt cagactcatg cctgaaggta taccacatta ggtaggaaga ttgcttggcg
     8221 cctcactcgt cgtggactgg atgcgtggaa gtgacggcgt cgtaactgag cttgatggca
     8281 catggtacag cgttgccatt tcctcaacgc tcataatgtt gtagtcgccc acgtacggtg
     8341 gatggaagaa tgcgcgcatg cggtaataca agagcaccat atggttcatg tgctctgtgt
     8401 ggtgcccatg cgggtcctcc catggatagt cgttgtagtg attggaccaa cggccgtctg
     8461 caggcataag cgagttcgcg ttctccgagt tgaacggctt gaagatgttt accaagacgc
     8521 cgcccatggt gccctgcgcc acatcctttg gtgcgagata gataccacga ataccgacgt
     8581 cgaacgcctg ctttccggtg ttcttctcaa tggcctcgat acgcgttgcc atcgcacgcg
     8641 tcgggtttgg gtaggaaatt gaagtgcgct tcacgccgtt ggcgtccacg tactcgttct
     8701 ccaccttatc ttcttcacgg agcttcttga tctcttcttt agcagaatct ttccaggacg
     8761 ctggttttga atcccacggc tttccctgat gcacgcgcac cacgaactga agccatagct
     8821 gttccttcgg tcccaaggat cccataatct ctatcatctg cgccaaaggg tcgatctgct
     8881 cttcaggctt ctgtacctga tcaagaccgt actccacgta cgttttcaac gggtacggat
     8941 ccggctttgt cttcctgaac tcgaaggccg tcatggtgta tggctcatgg gacggatcaa
     9001 ccaggcggct gtagtccata gcctcgataa cctcaacgcc agggtactgc gcgtacaggt
     9061 agctctcgag tgcgcggcgc cagccggtgc gcagccatac aaagtaatgc acacgcccgc
     9121 cgatagatac gatctcgtac gagtaccacg gacgtgcacg cccctgaatg atgcgcttgt
     9181 accacgtgtt ctcgcctccg gcgatgtgca tgctcgagaa cacggtttcc attgcaagcg
     9241 gggtgcgcct cgtgtcgcgc ggcatcttga tctcaagcaa cacggtctcc tgcttcgcga
     9301 tccatgcggt cttatttgca tcaatgaatc tgcgcaagcc gaaatgtaac aagagcgccg
     9361 ggatccaaag cggacttaga aagagtagga attggaagtt cgttatagtc tggctcggtg
     9421 aaaagaaaaa gaatgcaacg agctccgcgc aaaacgcgat gagtaacggc ttcagctcaa
     9481 cggagctgcc tccgtcctca tgtgccttat gcatccaatg ctgaatagct gagagtcccg
     9541 ggagtccgcc tccgccgtgt ccgcctgctg acatgcagga agtatagcag cgaaagaaca
     9601 cgagccccgg aagaatccgg ggctcgtgtg agtagagatg ctgttaggcg acctggtagg
     9661 tgccgtcttt ctggcgcttg aagtgacgcg tgtcgccgag gttcacgagg atggtgttgt
     9721 ccttcacata gcgaaccttc ttcacctctt cgatgatcgc gtcgcgcgtg agcggaccat
     9781 gcttctcaag cacctcgcga atcacgtcgc gcacgacgcc cggctggtag ccccactcag
     9841 cgagcgcgta gaggccgcga cccacgagca cgaatcgaga gtccttaatg agctcgttat
     9901 gagtggtcgc cacgtgcgcc ttcttgttga agacctcgac gatagaagac gcaacgtccg
     9961 caaagtgcat cggctcaccc ttctgcttta taacgagata cgcgtagtcg cgaataccct
    10021 tcgtacggat cgccggcgcg tgcgctttgc cccactcaga aagcggattc tttgcgatag
    10081 tctttgaaat ggtgagccag cgcttcagga tctcctcgtt ctggtacgcc tcgttcacgc
    10141 cctttaactc ctcgaggaag cggttgatga tgtcgccttc ggaaaggacc tctgcatcgt
    10201 caagcgtctc gtagaggcga gagagcgcgg tgtgtaccgc ctcggcggtc ttatggtcta
    10261 cgtgccaacg cgcgtagaaa tcgtcggtct cgcgctcacg gaagaatgcg ctgcctacaa
    10321 cgagaaggaa gcggaagcgg ttctttgctt tctcgtctga agcgagacct gcgagaagca
    10381 catcctcagg aacgatgccg ccctggccct cgatatggga cgcgagctcg tcaaaggctt
    10441 cctgagactc cttgaaagcc ttcgaattgc ggatggactc aatacctgcc gcctcgatct
    10501 gacgtacacg ttcacgcgtg atgctccagc gctcgccgat agcctcaagc gtctctcggc
    10561 gctgcgcagt cccaagaccg aaacggaggg tgagcacctc acggccgcgc tccggaacct
    10621 ccgaaaggag cttcttcacg agcgctaccg tgtcgaacga gagggatgcg ctggctgcct
    10681 taggtgcctt cgccttctta gcggctggga ccttctttga agcggctgcc ggcttcttcg
    10741 ctgatttcgc catataatta gtgtggtatt cttataccag attgcctaat aatcgtcaaa
    10801 tctagcctat aaccaggctc agaatccttc ctggcacgta gaccgccctt gagatagctc
    10861 caattgtgag ggcttcacgt acgaattgga gaccccgagc ggctgcgata acctcctctt
    10921 cggacgcagt ttttgcgagt acgacttccc cgcgcttctt cccgttcacc tgcaccacga
    10981 tcgtcatggt gtcgtcttct ggcacctcgc gagtaggcca ggatgcctga tgcactgacc
    11041 cttcgttgcc gagctcctgc cagagatgat cggcaagatg cggcgcaagc ggcgcaagaa
    11101 gttgcaagag ctcgccgtac gcttccttcg acacctgttc ctgcttctcc agatcgcgca
    11161 cgagcaccat gagcgcagat atagcggtgt tgtacttgaa gcgctcgata tcctcggaga
    11221 cctttgtggt tgcctgcgcg agcgcctttg cctcggcagg tgccgcctct ccttccgtta
    11281 ccttctcctt gagcgcatag atgcggtcaa ggaacttacg catggctgcg gcaccatcaa
    11341 ggttccatgg ataggtgccc ggctcgttat acgggccaat aaatgcgagg tacatgcgca
    11401 ccgcgtctgc gccgagctcc ttcacgacgg aatccgggtt gatgacgttg cccttggact
    11461 tcgacatctt cgcgccatca ggaccgagga tgagaccgcg gttgaagcgc tcaacgaatg
    11521 gttccgcgac cggtacgagt gcgaggtcgt agagtgcctt cacaaagaag cgtgagtaga
    11581 ggaggtgcat cgtggtgtgc tcgcttccgc ctgagtagcg gtttacgggc atccatcgct
    11641 tcatgacgga ctgatccgag aagtcgtgct cgtcctttga gtccagatac ctgaggaaat
    11701 accacgaaga atcaacgaac gtatcgagcg tgtcgtactc tggggtccaa ccctcaccaa
    11761 agatgcgcgt cacacgctcc tgaagctcct ttgaggttgc aagtggcgac ttcccggacg
    11821 gcgtgaagtc gacatccgtc gggagaagcc atggtaggtg ttccgccggt atcgcatgcg
    11881 gattgccttc cggatcgtat acgaccggta ttgggcagcc ccagtatc
//
LOCUS       DASWXU010000023         9757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_434035, whole genome shotgun sequence.
ACCESSION   DASWXU010000023 DASWXU010000000
VERSION     DASWXU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434035"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGE48_00730"
     CDS             complement(<1..281)
                     /locus_tag="VGE48_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041937.1"
                     /translation="MQPMRGMSLVDVIVGSALVLIVFVALLGLLRTSLLVSSSAKAKA
                     GATAVATTQMEYIRSLDYATVGTIGGIPAGPVAQYATTTMNGIPYAVRT"
     gene            complement(352..816)
                     /locus_tag="VGE48_00735"
     CDS             complement(352..816)
                     /locus_tag="VGE48_00735"
                     /inference="COORDINATES: protein motif:HMM:NF025717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HEY1041938.1"
                     /translation="MFRHWTEEQRTRLDAVGSLAEAGDLAVEVLSRMKEREQAIIQVC
                     GPMSTGGLGSLEKNMERFAHAIGALQDRGFLVFNQIPFQGAIVRLTDHHAGGEYCIDI
                     LEVFYRKIFECRHISRAMFLPDWESSTGARWEREFVAARGIPVDEFPERWLV"
     gene            complement(877..2088)
                     /locus_tag="VGE48_00740"
     CDS             complement(877..2088)
                     /locus_tag="VGE48_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1041939.1"
                     /translation="MKFRVTAKTSDGSEVHRVIEAPTRFAVYGEMEKEGATVISVEEA
                     KGGNAPAWTQITFGSGIKTDQKITFTKNLAAMLSAGLPLTRALSVIERQATNKYLKKV
                     VAALSEEVKKGSSFHEALAKHPKVFSELFIAMTKAGEESGTLADSLSVVARQMEKAFN
                     LQKKVKGAMIYPMIILSAIVIIGVLMLIYVVPTLSNTFKELGVELPAATKAIIDTSDF
                     MASNALLTMGILIAAFAGIFFFVRSKPGARIVLMLALRMPVIGELVRETMSARAARSL
                     SSLLSSGVEMLSALKISGEVVGDNSFGKVLREAEERVRKGEALSASFIDHPKLYPVFV
                     GDMIAVGEETGKVADMLGQVAQYYETDVEERTKDLSTIIEPLLMLFIGGAVGIFAMAM
                     ITPIYSLSDKI"
     gene            complement(2168..2506)
                     /locus_tag="VGE48_00745"
     CDS             complement(2168..2506)
                     /locus_tag="VGE48_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041940.1"
                     /translation="MKKTVTGLLIAVVIAAAAIWVSGMIPFVSLDNGEGVPGGFWPFA
                     IAMAVAAVLYWWATETTAFVIAVIGLAIALLCRWGGFEMLQDIGWLVLFGGVSAGILL
                     AAGPKPRPGH"
     gene            complement(2597..4351)
                     /locus_tag="VGE48_00750"
     CDS             complement(2597..4351)
                     /locus_tag="VGE48_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY1041941.1"
                     /translation="MQVSESELKEFILDSGLVSRKDVDAAEEEAKRRGQSIGDILVSQ
                     GLLSSDSLRRIKAYVLGIPFVNLKDKKIDLNVLSLIPEPIARTHSIIAFKKEGDSLEV
                     AMLDTEDLPSIDSVRKKTGLKILPRLTDDESLKNVLLQYQKSLKEEFGDLISTEAGRI
                     NLIKDAGAGDDASGEDLKKMAEDLPIVRIVDTLLRHAIIQKASDIHIEPMEQEVLVRY
                     RIDGVLHDAMTLPRTASAGITARIKVLANLKLDEKRLPQDGRFKMETETDKVSFRVSM
                     LPTYYGEKIVMRLLRETGEGFTLDVLGFHGDTMERIHKATKQTTGIILISGPTGSGKT
                     TTLYTILDILNTPDVNISTIEDPIEYQMKRVNQTQVKPEIGFTFANGLRSLVRQDPDI
                     IMVGEIRDGETASLAINAALTGHLVLSTIHTNSAAGAIPRLIDMGVEPFLLVSTLRVI
                     VGQRLVRRLCDDKEQFALDKEERAKVTSDDKFDAVLKALTDEKLVKAGTKIDNVPFHG
                     PKPSSQCEDGYSGRIGIHEVLAVSPAIRDIILHSGTTDAIEAQARKEGMLTMLEDGIY
                     KSARGITSIEEVLRAVSE"
     gene            4841..6250
                     /gene="nusA"
                     /locus_tag="VGE48_00755"
     CDS             4841..6250
                     /gene="nusA"
                     /locus_tag="VGE48_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY1041942.1"
                     /translation="MLDLKTINLVLGEFEERGITKETMIDAIEQAMATAYKKEYGKRG
                     QVIRGALDMNTGTVSFEQVKTVVDETLVRFPEEGEEINELPAHHPHFDREEEVAEGEL
                     PRYDAEKHMLLEDAQRIKRGAQLGEEIIFPLEPQDDFGRIAAQTAKQVVIQKIREAEK
                     LSILDEFGHKKGEVVTGIVQRAERGAIFVDLGRATGVIPYEEQIPGERYRPGERISAY
                     LYAVDEGFRGVYLRLSRSHPRFLTRLFEMEVPELAAGAIEIKGLAREPGSRTKIAIAS
                     NDPHVDPVGSLVGQRGVRVSTVMAALGGERIDIIEWSEDMASYIKESLSPATVLEVEL
                     NEEERRAKVTVSEDQQSLAIGRGGQNVRLAAKLTGWNIDIMSIGGENVADADGQQEET
                     ASAATDESVAEVENTEDMDPAVAAGIDPTETESMVTEDDSTDNSLTVEEETELADDGS
                     PVNPEDRDVTTESQADNQA"
     gene            6265..6789
                     /locus_tag="VGE48_00760"
     CDS             6265..6789
                     /locus_tag="VGE48_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY1041943.1"
                     /translation="MNLLHDIAPGSADQMNVVIEIPNGSKNKYEIDKETGMIALDRVL
                     HTAQSYPFDYGFVPQTLWDDGDALDVVLLTTQPLLPGILVHARPVAILPMVDGGEQDE
                     KVLAVPASDPRFNDIQDLGDVNKHTLKEISHFFETYKKIQNKEVTVGEWQGADAAKAA
                     FARAVEMYQSKKAA"
     gene            6904..7203
                     /locus_tag="VGE48_00765"
     CDS             6904..7203
                     /locus_tag="VGE48_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041944.1"
                     /translation="MENVPGENMQQDTAAPAPAPAPVAEEAKKGAAYGSIIGIVIIVA
                     VLVAGAFYVWGERLNEQQPQVPENGVRGEMLPDGSYPSVEGSLDVESGENGPQPR"
     gene            7282..7893
                     /locus_tag="VGE48_00770"
     CDS             7282..7893
                     /locus_tag="VGE48_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY1041945.1"
                     /translation="MEHTRKNPRASMLVPMVVEKSQFGERGYDIYSRLLKDRIIFLGG
                     PIDNDTANLVIAQLLFLESEDPKKDIYLYINSPGGSVTSTLAMIDTMNHVKPDVATVC
                     VGMAASGGAWTLAAGTKGKRFALPNAEIMIHQPLGGAEGQATDIEITAKQILKLRERL
                     NKLMAGYTGQTVAKIEKDVERDFFLTADEAKKYGLVDVVYKSK"
     gene            8028..9557
                     /gene="rny"
                     /locus_tag="VGE48_00775"
     CDS             8028..9557
                     /gene="rny"
                     /locus_tag="VGE48_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY1041946.1"
                     /translation="MSIKIVLSLLALSGLAGIALGYVLRLLIALGQRGSLELEVKQKM
                     LEAKERANRIIAEAEAKADSIEESRLSPLKEREDKLAKASERLAKREEFLDERERALV
                     AESEDVKNRENEAVNMKKEVERLREERRQELSRIARLSENEAKSLLIKDLEESYEESV
                     LARLQKLETHGRERLDEKARSILLAIIHRMSASVTNDVMTLSVQIPSEEVKGKIIGKE
                     GRNIKAFERATGVDIIIDDAPDRITLSSFDPLRRHTAKLALENLIEDGRIQPAKIEEA
                     VEKAKAEIAETIKQKGEEAAYEVGVVGLDPRLITLLGRLNFRTSYGQNVLRHSIEMAH
                     IAGMLAAEVGADVDIAKAGALLHDIGKALDHEVDGTHVEIGRRILEKYGVDRRVIQAM
                     QSHHEEYPYETPESMVVQAADAISAGRPGARRDTVERYLERLTDLERVALGFPGVEKV
                     YAISAGREIRIFVNPGKVSDIQTHRLARDIAERIQKELKYPGEIKVNVIRENRVVEFA
                     R"
BASE COUNT         2229 a         2804 c         2689 g         2035 t
ORIGIN      
        1 aacgtgcgca ctgcgtacgg gatgccgttc atggtagtag tcgcgtactg cgctaccggt
       61 cctgcaggaa tgccgccgat agttcccacg gtcgcgtagt cgagcgaacg aatgtattcc
      121 atctgcgtag tcgctaccgc agtcgcgcct gccttcgctt tcgcagaaga tgacacgagg
      181 agcgaggtgc gcaggagtcc gaggagtgcg acaaacacaa taagcacgag cgcagacccg
      241 acgataacgt ctacgagcga catgccgcgc atcggttgca tagacgcatg ataccgcacc
      301 aatgtatgtg aaaggccgcg cgaacatatc gcgcggcctt gctgcttgtc gtcacaccaa
      361 ccagcgttcc ggaaactcat ctaccggaat accgcgggct gcaacaaact ctcgctccca
      421 gcgagctccc gtggaacttt cccagtcagg gaggaacatg gcgcgactga tatgacgaca
      481 ctcaaagatc ttccgataaa agacctcgag gatgtcgatg cagtactcgc ccccagcatg
      541 atgatcggtc agccggacga tcgcgccctg gaacgggatc tggttgaaga ccagaaatcc
      601 tcgatcctgg agcgcgccga ttgcgtgcgc aaaccgctcc atgtttttct cgagagatcc
      661 gagccctcca gtcgacatgg gtccacagac ctgaatgatc gcctgttctc gttctttcat
      721 tcgcgaaaga acctctacgg ccagatcccc tgcttccgca agggatccta ccgcgtcgag
      781 ccgcgtgcgc tgctcttcag tccaatgcct gaacaaagta tcctcctcgc tccagttcta
      841 tcccggagca tacgcgctcc aaagtttgct gtcgagctag atcttatcgg agagcgagta
      901 aataggcgta atcatcgcca tcgcaaatat acccacggct cctccgatga agagcatgag
      961 gagcggctcg atgatggtag agaggtcctt ggtgcgctcc tcgacatcgg tttcgtagta
     1021 ctgcgctacc tgcccgagca tgtctgctac ctttccggtc tcttcgccca ccgcaatcat
     1081 gtcgcctacg aatactggat agagcttcgg atgatcgata aacgatgcgg aaagcgcttc
     1141 gcccttacgt acacgttcct ccgcctcccg gagcacctta ccaaaactgt tgtctcccac
     1201 cacctcaccc gagatcttaa gcgcagagag catctcaaca ccggaagaaa gaagcgacga
     1261 caaagagcgt gccgcacgcg cactcatagt ctcgcgcacg agttccccaa taaccggcat
     1321 acgtagtgca agcatgagaa cgatccgtgc gcctggcttc gaacggacga agaagaatat
     1381 gcctgcgaag gccgcgatga ggattcccat ggtaagaagt gcgttcgagg ccatgaaatc
     1441 agacgtgtcg atgatagcct tcgtcgcagc aggaagctcc acgccgagct ccttgaacgt
     1501 atttgaaagg gtcggcacca cgtagatgag catgagtaca ccgatgatca cgatcgcgga
     1561 aaggatgatc atcggataga tcatggcgcc cttaaccttc ttctggaggt taaatgcctt
     1621 ttccatctgg cgcgccacaa ccgagagcga atcagcgagc gtacccgatt cctcgcctgc
     1681 tttcgtcatg gcgatgaaga gttctgagaa cactttcggg tgcttcgcga gcgcctcatg
     1741 gaacgaggaa cctttcttta cctcctcgct gagcgccgcg acgaccttct tcaggtactt
     1801 attagttgct tggcgctcga taacggagag tgcgcgggta agcggaagac ccgctgaaag
     1861 catcgcagcg agattcttcg tgaacgttat cttctggtcg gtcttgatgc ctgagccgaa
     1921 ggttatctgc gtccaggcag gagcgtttcc gcccttcgct tcttcaacgg aaataaccgt
     1981 tgctccttcc ttctccatct cgccatacac tgcaaagcgc gttggtgctt cgataacgcg
     2041 atgcacttcg cttccatcac tagtcttcgc ggttacgcgg aacttcatgc gtgaatcgta
     2101 gcatgcagat atgtgaacgg gccgggttcc ttgggaggaa cccggcccga ggccagacga
     2161 aggccgatca gtggccggga cggggcttcg gccccgcggc caacaagatg ccggccgaga
     2221 cgccgccgaa gagcaccagc cagccgatgt cctgcagcat ctcgaagccg ccccagcggc
     2281 acagcagcgc gatcgccaga ccgatgacgg cgatgacgaa ggcggtggtc tccgtcgccc
     2341 accagtacag cacggccgcg acggccatcg cgatcgcgaa gggccagaag cctccaggca
     2401 cgccctcgcc gttgtccagg gacacgaacg ggatcatgcc ggagacccag atggcagccg
     2461 ccgcgatgac gacggcaatc aggagacctg tgacggtctt tttcatattc actcctccga
     2521 gaacacactg ccggggatcc ggtcagagag gatgtgttcc gcgtccatta tactccccca
     2581 cccttataaa aacaacctac tcggacaccg cacgaagcac ctcttcgatg gaagtaatgc
     2641 cgcgcgcgct cttgtagata ccatcctcga gcatcgtgag catgccctcc ttgcgcgcct
     2701 gcgcttcgat cgcgtcagtg gtgccgctat ggaggatgat gtcgcgaatt gcaggtgata
     2761 ccgcgagcac ttcatgaatg ccgatacggc cagagtaccc gtcctcacac tgagacgacg
     2821 gctttggtcc atggaacggc acgttatcga tcttcgtacc tgcctttacg agcttctcgt
     2881 ccgtaagtgc tttcaacacc gcatcgaatt tgtcgtctga agtgaccttc gcgcgctctt
     2941 ccttgtcgag cgcgaactgt tccttatcgt cgcaaagacg acgcacgaga cgctgaccga
     3001 cgatcacgcg gagcgtagag acaaggagga acggttctac acccatgtcg atgagacgcg
     3061 ggatggcgcc cgctgctgag ttcgtgtgga tggtcgagag cacgaggtgt cccgtgagcg
     3121 ccgcgttgat ggcaagggac gcggtctctc cgtcgcggat ctctcccacc atgatgatgt
     3181 ccgggtcctg acgcacgagg gaacgaaggc cgttcgcaaa cgtgaatcca atctctggct
     3241 tcacctgcgt ctgattcacg cgcttcatct ggtactcgat cgggtcctca atcgtggaga
     3301 tgttcacgtc cggcgtattg aggatgtcga gaatcgtgta caaggtcgtc gtcttaccag
     3361 aacccgtcgg tcccgagatg aggatgatac ccgtggtctg cttcgtcgcc ttatggatgc
     3421 gctccatggt gtcgccatgg aacccgagca catcgagcgt gaagccctcg cccgtctcac
     3481 gaaggagacg catgacaatc ttctcaccgt aataggtcgg cagcatcgac actcggaacg
     3541 acaccttgtc ggtctccgtc tccatcttga agcggccgtc ctgcggaagg cgcttctcgt
     3601 cgagctttag gttcgcgagc actttgatgc gagccgtgat gccagcagac gcggtgcgcg
     3661 gaagtgtcat cgcgtcatgt agcacgccgt cgatgcgata gcgcaccagt acttcctgct
     3721 ccatcggctc gatatggatg tcggatgctt tctggataat ggcgtgacgc agcaaggtat
     3781 ccacaatgcg cacaatcggc aggtcttctg ccatcttctt caggtcctcg cccgacgcat
     3841 catcccctgc ccctgcatcc ttaataaggt tgatgcggcc agcttcagtc gaaataagat
     3901 cgccgaactc ctccttaagc gacttctggt actgcaggag tacgttcttc aaagactcgt
     3961 cgtctgttag gcgcggaaga atcttaagtc ccgtcttctt acgcactgag tctatggacg
     4021 gaaggtcctc cgtgtcgagc atcgctacct cgagcgagtc tccttctttc ttgaacgcga
     4081 tgatggagtg tgtacgcgca attggctcag gaataagcga gaggacgttg agatcaatct
     4141 tcttatcttt gaggttcacg aacggaatac cgagtacgta tgccttgatg cggcggagcg
     4201 agtctgatga aaggagtcct tgggacacga gaatgtcgcc gatggactga ccacggcgct
     4261 tcgcttcttc ttccgctgca tcgacatcct tcctggagac gagtccagag tcgagtatga
     4321 attccttaag ttcgctttca gagacctgca tgccgcttta ttagtaaaag tataacgcgc
     4381 cgatgtaaaa catcgacgcg caacacattc ttgtgagggg attaaacccc aagaggcact
     4441 ggtgctggtt ctccgttcag gaagaccgcg acaccgtccg tgccgacgaa ctgatcacgc
     4501 acataccagc gacacccgca aaacgtgcat tcgagggttg catcagcgtc gaccgggtca
     4561 tgagaaacaa tcctgacctc agactcgtcc cctccgcagg ccgtgcagcc ttcgatagtc
     4621 agccaggcgt ggggcccgga tattggcgac ataagcatgg ttactctccc ctactgctct
     4681 tatcgcatca tacacccgtt tcattgaccc tttacctata tatggtatgc ttcccctatc
     4741 tggaatgtcc gcacccgcgg gcattctcta ttttagagaa acccggatgc gtgcgaccat
     4801 acccttatat acggtgcgaa caatcacata gagaagactt atgttggacc tcaagaccat
     4861 aaacctggtg ctcggcgagt tcgaagagcg cggcatcacg aaagagacga tgatcgacgc
     4921 tatcgagcag gcgatggcaa cggcgtacaa gaaagaatac ggaaagcgtg gacaggtaat
     4981 ccgcggggct cttgatatga acacgggtac cgtgtcgttc gagcaggtga agaccgtcgt
     5041 ggacgagacc ctcgtacgct tccctgagga gggcgaggag attaacgagc tcccggcaca
     5101 tcacccgcac ttcgatcgtg aggaggaggt cgccgaaggc gagcttccac gctacgacgc
     5161 agagaagcac atgctccttg aggacgcgca gcgcatcaag cgcggtgcac agctcggcga
     5221 ggagatcatc ttcccgcttg agccgcagga tgacttcggc cgcatcgcag cccagaccgc
     5281 aaagcaggtt gtaatccaga agatccgcga agcggagaag ctctctatcc ttgatgagtt
     5341 cggccacaag aagggcgagg tcgtaacggg tatcgtgcag cgcgctgagc gcggcgctat
     5401 cttcgttgat cttggtcgcg caactggtgt cattccctat gaggagcaga ttccaggtga
     5461 acgctaccgt ccgggcgagc gcattagcgc atacctctat gccgtagacg aagggttccg
     5521 cggcgtatac ctccgcctct cacgctcaca ccctcgtttc ctcacccgtc tctttgagat
     5581 ggaggtgccg gagcttgcgg cgggcgctat cgagattaag ggtctcgcgc gcgaaccagg
     5641 ttctcgcacg aagatcgcga tcgcctcgaa cgatccgcac gtggatccgg ttggttctct
     5701 cgtaggtcag cgtggcgtac gcgtctcgac cgtgatggca gcactcggcg gcgagcgtat
     5761 cgacatcatc gagtggagcg aggacatggc gtcctacatc aaggaatccc tctcccctgc
     5821 taccgtgctt gaggttgagc ttaacgagga agagcgccgt gcgaaggtaa cggtatccga
     5881 ggatcagcag tcgcttgcga ttggtcgtgg tggccagaat gtgcgccttg ccgcaaaact
     5941 cacgggctgg aacatcgaca tcatgtcgat cggcggcgag aacgtcgctg atgcggacgg
     6001 acaacaggag gagaccgcat ccgctgctac tgacgagtct gttgccgagg tagagaatac
     6061 cgaggacatg gacccagcag tcgcagcagg tatcgatccg accgagactg agtccatggt
     6121 taccgaggac gattcgaccg acaacagcct tacggttgag gaggagaccg aactcgctga
     6181 cgacggctct ccggttaacc cagaggaccg cgacgtcacg accgagtctc aggcagacaa
     6241 ccaggcataa cccacccatc tctcatgaac cttcttcacg acatcgctcc gggctccgca
     6301 gatcagatga acgtggtcat cgagatcccg aacggttcca agaacaagta cgagatcgac
     6361 aaggagaccg ggatgatcgc gcttgatcgc gtgcttcaca ccgcgcagag ctacccgttt
     6421 gactacggct tcgtgccgca gaccctttgg gacgacggtg atgcgcttga tgtggtgctc
     6481 ctcaccaccc agccgcttct tccaggcatt ttggtccacg cacgccctgt agctatcctt
     6541 ccgatggttg atggtggcga gcaggacgag aaggtgcttg cagtgccagc atcggatcct
     6601 cgattcaacg acatccagga tcttggggac gtgaacaagc acactctcaa agagatctct
     6661 cacttcttcg agacgtacaa gaagatccag aataaggaag tgaccgtcgg tgagtggcag
     6721 ggagcagatg ctgcaaaggc agcattcgcc cgcgccgtag agatgtatca gagcaagaag
     6781 gctgcatagc ctcatctctc aggaaaagac cccggaattc cggggtcttt tcctatcccc
     6841 acgaattctt cgggaggcaa ttaggtcacc tatacttaag tacatagcta acccacattc
     6901 atcatggaga acgtaccagg agagaacatg cagcaggata cggccgctcc ggcgcctgct
     6961 cctgccccag tagccgagga ggcgaaaaag ggtgccgcat acggctccat cattggcatc
     7021 gtgatcatcg tcgcggtcct tgttgcaggc gcgttctacg tctggggcga gcgtctcaat
     7081 gagcagcagc cgcaggttcc ggagaacggt gttcgtggcg agatgcttcc agatgggtct
     7141 tacccttctg tcgaaggttc tcttgatgtc gagtccggcg agaacggccc tcagccgcgc
     7201 taacaaacag tgtagtgaaa agcgcccgct gatgcgggcg cttttcttat atatgccgtc
     7261 atttggtact atggccctac tatggaacac acacgtaaga accctcgcgc ctcaatgctc
     7321 gtaccgatgg tggttgagaa atcgcaattc ggcgagcgcg gatatgacat ctattcccgt
     7381 ctcctcaagg accgcatcat cttccttggc ggcccaattg ataacgacac cgcgaatctc
     7441 gtaatcgcac agctcctctt cctcgaatcc gaagatccga agaaggatat ctatctctat
     7501 atcaactccc cgggaggatc cgtcacgtcg acactcgcga tgatcgacac catgaatcat
     7561 gtgaagccag atgttgctac cgtgtgtgtc ggtatggcag catccggcgg tgcatggact
     7621 cttgccgcag gcaccaaggg taagcgtttc gcactcccga acgcagagat catgatccat
     7681 cagccacttg gcggcgctga gggccaggcg accgacattg agatcactgc aaagcagatc
     7741 ctgaagctcc gcgagcgact taataagctc atggcgggtt acaccggcca gacggtcgca
     7801 aagatcgaaa aagacgtcga gcgtgacttc ttccttaccg cggacgaagc aaagaagtac
     7861 ggccttgtgg acgtggtcta caagagtaag tagctcccct ctctccgaaa cggcctcgcc
     7921 ttacgcgagg ccgtttcgtt tgcaaaaacg ctaaaaagtg ttatttttca atggttttca
     7981 ggctccggta cgactgaaac ggctgaaata ctacgcctca cacctatatg tccataaaga
     8041 tcgtcctctc gctcctcgcc ctctccggcc ttgctggaat cgcgctcggc tacgtcctcc
     8101 gtcttctcat cgcactcggc cagcgcggat cacttgagct tgaggtgaag cagaagatgc
     8161 tcgaagcgaa ggagcgcgca aatcgcatca tcgcagaggc agaagccaag gcagactcta
     8221 tcgaggagtc ccgtctctcc cctcttaagg agcgtgagga caagcttgcc aaagcatcag
     8281 agcgccttgc gaagcgcgag gaattcctcg acgagcgcga gcgtgcactc gttgctgaga
     8341 gcgaggacgt aaagaatcgc gagaacgagg ccgttaacat gaaaaaagag gtggaacgcc
     8401 ttcgcgagga gcgccgtcag gaactctcac gcattgcgcg cctctctgag aacgaagcca
     8461 agtctctcct catcaaggac ctcgaggaga gctatgagga aagcgtgctt gctcgtcttc
     8521 agaagctcga gacccacgga cgcgagcgtc ttgatgagaa ggctcgttcc attctcctcg
     8581 caattattca tcgtatgagc gcgagcgtca cgaacgacgt aatgacgctt tccgtgcaga
     8641 ttccgtccga ggaggttaag ggtaagatca tcggcaagga gggacgcaat attaaggcct
     8701 ttgagcgtgc caccggcgtc gacatcatca tcgacgacgc tccggaccgc attaccctct
     8761 cttcgttcga tccactccga cgccataccg caaagctcgc gcttgagaac ctcattgagg
     8821 acggccgcat ccagcctgcg aaaatcgaag aggcggtaga aaaagctaag gcggagatcg
     8881 cagagactat taagcagaag ggcgaggaag cggcgtacga agtaggcgtt gtaggccttg
     8941 atccgcgcct tattacgctt cttggccgcc tcaacttccg cactagttac ggccagaacg
     9001 tgcttcgtca ctcaattgag atggcacaca tcgcgggcat gcttgccgct gaagtgggcg
     9061 cagacgtcga cattgcaaag gctggtgcgc ttcttcacga tatcggcaaa gcacttgatc
     9121 acgaagtaga cggcacacat gtggaaatcg gtcgccgcat cctcgagaag tacggcgtag
     9181 atcgtcgcgt catccaggca atgcagtcgc atcatgagga gtatccatat gaaacgcctg
     9241 aatcaatggt ggtgcaggca gcggatgcta tctctgcagg ccgcccgggc gcacgacgcg
     9301 atactgtcga gcgctatctt gagcgcctta cggatctcga gcgcgttgcg ctcggattcc
     9361 caggggttga gaaggtatac gctatatccg caggtcgcga gatacgcatc ttcgtaaacc
     9421 caggcaaggt ttctgacatc cagacacatc gcctcgcgcg cgatatcgct gagcgcattc
     9481 aaaaggagct taagtatcca ggcgagatca aggtgaacgt gattcgagag aaccgagtcg
     9541 ttgaatttgc gcgataacgt aacttttcta ataaaacagc ctcgctactg cgaggctgtt
     9601 ttcgtaaaaa gtattgattt caccttatcc ccgttatttt tcaaccccta ttgcgtaaaa
     9661 tatggcgttt tctatacttt tcccgtagcc ctctcatagg gcttgtgagc cctaagagga
     9721 ttaaccccta aaagaacgac acatgaacaa ggcagat
//
LOCUS       DASWXU010000024         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_326831, whole genome shotgun sequence.
ACCESSION   DASWXU010000024 DASWXU010000000
VERSION     DASWXU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326831"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGE48_00780"
     CDS             complement(<1..221)
                     /locus_tag="VGE48_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041947.1"
                     /translation="MEHTLYLSQVLGLFLIIVGLAIMLRKRYYVPVVGEFIEDRFMRM
                     VMGILELLGGLFLVLAHNDWSTLPASIISA"
     gene            complement(298..1374)
                     /locus_tag="VGE48_00785"
     CDS             complement(298..1374)
                     /locus_tag="VGE48_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1041948.1"
                     /translation="MSTFKQSLAAFAFIALIATSFPVSVNAQSRSDWEDYYREYLESR
                     GRTRSSVDDDDRRTATRERYSSATQRAINKLDDDPVDEIPIPVMFGVAPRNIYSDFGD
                     PRGGGTREHEGQDIIAPRGTPIASPTDAVVTRTGNGASSGITVTTRNPGGETFIYMHL
                     DEVAEGIKAGTVLEPGDILGFVGDTGNAKGGVTHLHFEIRDGREATDPYPRLDREFTL
                     KERVDGITDYLEELDSDDREEFAELMVKNYRSIFVQARAQSVELPDEIAEALGAVSIV
                     DANAAVRDLTLGSQGDDVTVLQVALIAADKGPAAKALAGAGATGYFGAMTQAALAEYQ
                     RALGITPASGYYGPLTRARMAAGA"
     gene            complement(1658..2080)
                     /locus_tag="VGE48_00790"
     CDS             complement(1658..2080)
                     /locus_tag="VGE48_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY1041949.1"
                     /translation="MRTLITGSYLNRWSGFAPTVLRVVTGIIFALHGWQKLEGGLPMT
                     AGMLEGMGFPMPMVFAVLLIAAELGGGILLILGVLTRISAKFLAIVSLVALFAVHFKN
                     GFFMSTGGYEYILLLLAASVSLMLSGAGKWALGRGMWK"
     gene            complement(2154..2390)
                     /locus_tag="VGE48_00795"
     CDS             complement(2154..2390)
                     /locus_tag="VGE48_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007018575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY1041950.1"
                     /translation="MWYVYLLRCADGTLYTGVTTDIERRMKEHNESREGAKYTRARRP
                     VSLAHQETFPTRSEACAREAAIKKLSKGEKEALL"
BASE COUNT          558 a          681 c          688 g          500 t
ORIGIN      
        1 gccgagatga tacttgccgg aagcgtgctc cagtcgttat gcgcaagcac gaggaagaga
       61 ccaccgagaa gctcgaggat gcccatgacc atgcgcatga agcggtcttc gatgaactcg
      121 cctactaccg gtacgtaata gcgcttacga agcataatcg cgagacccac gatgataagg
      181 aaaagcccga gcacctggga aagataaagg gtgtgttcca tatccttaag tataggattc
      241 ctcaaatgcg aaaatcccgg acctgtgcat acaggtccgg gatttctcga gatgaggcta
      301 cgcgcctgca gccatgcggg cgcgtgtcag tggcccgtag tagccgcttg ccggcgtaat
      361 accgagcgcg cgctgatact cagcgagcgc tgcttgcgtc atcgcgccga agtagccggt
      421 tgcgcctgcg cctgcaagtg cttttgccgc aggacccttg tcagcagcaa taagtgcaac
      481 ttgaagcact gtcacgtcgt ctccttggga acccagcgtg agatcgcgta cggctgcgtt
      541 cgcatcaacg atggatactg cgccaagcgc ttccgcaatc tcgtcaggaa gctcgacgga
      601 ttgtgcgcgg gcctgcacga agatgctgcg gtagttttta accataagct ctgcgaactc
      661 ctcgcgatcg tcgctatcga gctcttccag ataatctgtg atgccatcaa cacgctcctt
      721 gagggtaaac tcgcgatcaa gacgcgggta cgggtcggtc gcctcgcggc cgtcccggat
      781 ctcaaagtga agatgtgtca cccctccctt cgcgttccca gtgtcaccca cgaacccaag
      841 aatatcgccc ggttcaagca cggtccctgc cttgatacct tcagcaacct cgtcgaggtg
      901 catgtagatg aaggtctcgc ctcctggatt tcgtgtggta acggttatgc cgcttgaagc
      961 gccgttaccc gttcgcgtaa cgaccgcgtc tgtcggagac gcaattggcg taccgcgtgg
     1021 cgcgatgatg tcctggcctt cgtgctcacg cgtgcctcct ccacgagggt cgccgaaatc
     1081 ggaatagatg ttcctcggcg cgacaccgaa cataaccggg atcggtattt catcaactgg
     1141 gtcgtcatcg agcttattaa tggcacgctg cgtggcgctc gagtagcgct cgcgtgtcgc
     1201 cgtgcgtcga tcatcgtcgt caactgaaga acgcgtgcgt cctcgagatt cgagatactc
     1261 gcgatagtag tcttcccaat ctgagcgcga ctgtgcgttt actgagacag ggaacgaagt
     1321 tgcgatgagc gcaatgaagg cgaacgccgc aagcgattgt ttgaaggtac tcattagatg
     1381 ttcttacgtg agaaattctg tccgtcagac agctactgca taagacggac ataacctaat
     1441 gcacttacca cgattacgtt cttcatgaag atactaggag atgcgcattg cgcgtgcgag
     1501 acgtctgcgt gcaatgcgcc agacaatacg accaagcacg aggaaggcca gcctgcgtag
     1561 ggatcgaatc atgtacatac gctagcggaa gaaacatgaa gcactcatga cgaaaaaggc
     1621 ccgcatgtgc gggccttttt ctaagagatt cgtacgctta cttccacatg ccgcggccga
     1681 gcgcccactt tccagcgccg gaaagcatga gcgacaccga agcagcgaga aggagaagga
     1741 tgtactcgta cccgccagtc gacatgaaga agccgttctt gaagtgcacc gcaaatagcg
     1801 ccacgagcga cacgatcgcg aggaacttcg cagagatgcg cgtaagcacg ccgaggatga
     1861 ggagaatacc gccaccaagc tctgccgcga taagaagaac cgcgaagacc atcggcatcg
     1921 ggaagcccat accttcgagc atgcctgccg tcatgggaag tccgccctcg agcttctgcc
     1981 aaccatggag ggcgaagatg atacctgtta cgacacgaag cacggtagga gcgaaaccac
     2041 tccagcgatt aaggtatgaa ccggtgataa gagtacgcat agatgatagt gatgctgcta
     2101 acccttagat tgtatcgtct ctccgtgaag ggttcatggg gacagtgcac atcctagagt
     2161 agggcttcct tctccccttt cgagagcttc tttatggcag cttcccgggc acaggcctcg
     2221 ctgcgcgtcg ggaacgtttc ctgatgcgcg agcgataccg gacgccgggc ccgggtgtat
     2281 ttcgcgccct ctcgggattc gttatgctcc ttcatgcggc gctcgatatc ggttgttacg
     2341 cccgtataga gcgtgccgtc ggcgcaccgg aggaggtaca cgtaccacat acgtggagta
     2401 tagcgaaaga aagcccgcgc cgtttcc
//
LOCUS       DASWXU010000025         7630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_384530, whole genome shotgun sequence.
ACCESSION   DASWXU010000025 DASWXU010000000
VERSION     DASWXU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384530"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..536
                     /locus_tag="VGE48_00800"
     CDS             252..536
                     /locus_tag="VGE48_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041951.1"
                     /translation="MCSHAFVALPNPGFLGQDGKVVTVKGFIRASDVDEVRLLLFAKD
                     CTQDKIDREGPWVVIERSVRVASYDGIRRHGANLAADVSKYFAPDEVTMH"
     gene            610..1419
                     /locus_tag="VGE48_00805"
     CDS             610..1419
                     /locus_tag="VGE48_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041952.1"
                     /translation="MSIPYVLLDKKVGQTPLAAIDAWREANPAYVTVSATYAGRLDPM
                     AEGKLLVLLGDECKNQERYRDLDKEYEIEVLLDLSTDTGDGLGIPMYGGTETVPETHA
                     RAKALRAQTGTHSVPYPVFSSKTVAGKPLFQYSLEGALDSIEVPSHKETIYRISELGY
                     AKLSKEALKERIQTMLSLAPRVDAPSKRLGDDFRQDRIRAAWNDLFDVIPDRSFFVLR
                     LRIICASGTYMRTLASRIGNALGTTGMALSIRRTRIGKFSRLFRIWTREFR"
     gene            1467..1685
                     /locus_tag="VGE48_00810"
     CDS             1467..1685
                     /locus_tag="VGE48_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041953.1"
                     /translation="MAEHERDRSLSEPRIRKAALLVSVCVAAVRDGKPIPEHCEVMGF
                     EVEEDGDIRFTSSVNMGAVIQDFEEPPP"
     gene            complement(1742..1813)
                     /locus_tag="VGE48_00815"
     tRNA            complement(1742..1813)
                     /locus_tag="VGE48_00815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1778..1780),aa:Glu,seq:ctc)
     gene            complement(1856..3139)
                     /gene="murF"
                     /locus_tag="VGE48_00820"
     CDS             complement(1856..3139)
                     /gene="murF"
                     /locus_tag="VGE48_00820"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY1041954.1"
                     /translation="MKALVKMLVAWYLATLARAILKKYRPLVVMVTGSVGKTSTKDAT
                     AAVLSNRFNLRASEKSYNSEFGVPLTIIGAKNPWENPIAWIRVFQEALALILLPNHYP
                     KLLVLEVGADRPGDLAKILRFADPDAVVVTRLPEVPVHVEAYASPQAVREEEFAPAYA
                     LTPGEPLIISADDPYAVSMSGRLPAKVSTFGFADGATVHLSGYSFYEEGEDCGMEAAL
                     EIEGKSFTLRVKDALGRSQLYAPAAGIACALALGMSPEDALQGASSYVSPAGRGRILR
                     GKDGALIIDDSYNASPAAVEEALSALSLIPGRSRRIAVLGDMLELGRYSVEEHRRVGT
                     LAATGADVVVAVGIRARAIAEAARDAGLPQDKVLSFDSSKDAAEALAGVVGKGDVVLV
                     KGSQGIRTERVAAALLHDEADRAKLVRQEREWQRR"
     gene            complement(3142..4872)
                     /locus_tag="VGE48_00825"
     CDS             complement(3142..4872)
                     /locus_tag="VGE48_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY1041955.1"
                     /translation="MRRAIRLRLRLLTFGLAAVALLLVTRLYFVQVVHGGEFGLRAER
                     QYMSASQELYDRGSIYFTRKDGSVLSAATLATGFTVAMSPKLVEDPEAAYAAINAIFP
                     IEREPFFQSVARKEDPYEVVAKRVPEDEGRAIEALDLPGISVSRERWRSYPGETRAAQ
                     SIGFVAFDDDDTIAGRFGLERYYEDVLSRGSTGVFGNFFAELFANIDNAVADGRGARE
                     GDLVTSIEPMVQEKLDQVLLSINAQYGSRETGGIIMDPKTGAIIALDTAPSFNLNDFQ
                     NGDSTHFGNPLVEHRYEFGSIVKALTMAAGLDSGVVGPTSTYNDTGCTTLNNKKFCNY
                     DLKARGGGTPMQEVLSQSLNMGAAYIALQVGHERFRGYFKDLGMNTETGIDLPSEIRG
                     DLANIETSPRDIEYATASYGQGIAQTPIAMIRALGALANDGQIVTPHLVSKVRLESGI
                     EKQLSWGMPERVFSSKAAEDTTRMLVEVVDSKLAGGTVKIPEMSVAAKTGTAQIAGPG
                     GKYAEGKYFHSFFGYFPAYEPRFIILLYTREPGGVQYASETLTHPFMELTHFLINYYE
                     IPPDRADYPS"
     gene            complement(4877..5155)
                     /locus_tag="VGE48_00830"
     CDS             complement(4877..5155)
                     /locus_tag="VGE48_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041956.1"
                     /translation="MPSKTIPYLGYTAAALVAAYLMLVAVTVSMAAWKTSLSIEVHET
                     EQDIARLESRFYAMVADIDRADPGSRGLEKPRSVTYATTQAAPTVSIR"
     gene            complement(5148..6098)
                     /gene="rsmH"
                     /locus_tag="VGE48_00835"
     CDS             complement(5148..6098)
                     /gene="rsmH"
                     /locus_tag="VGE48_00835"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY1041957.1"
                     /translation="MEGRHDTVLLEEAVDALDIQASDAVVDATLGGAGHFALILQKLG
                     KDGALMGIDADEDAITRASKVVAEVPESERPNVSLMHGNFRELATMLDEAGMTPDKVL
                     FDLGWSGFHLTRGRGFSFKVDEPLHMTYGDPAEGTTAADLVNHLSEASLADMLWSLGE
                     ERFARSIARAIVAARENGSLETTNDLVAAIEQGVPSWYRHRRIHPATKTFQALRIAVN
                     DELGALREGLSAAMERVPAGGRVAVITFHSIEDRIVKTMFREAVEAGKGSLLTRKPIA
                     PSVQETVRNPRARSAKLRTFVSGPRELKKRINLTSVPAYA"
     gene            complement(6102..6536)
                     /gene="mraZ"
                     /locus_tag="VGE48_00840"
     CDS             complement(6102..6536)
                     /gene="mraZ"
                     /locus_tag="VGE48_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY1041958.1"
                     /translation="MLIGEYRHTLDAKNRLSLPAKFRKELGASVIVTRGLDHCLFVYP
                     KAAWKKEAQKFSAHSSGTAAGRAFARLMLAGASEADVDSAGRILVPDYLKSYATLSGK
                     CVVAGVSDRVELWDEAAWEAYTKDIERDADSFAEALGAKALQ"
     gene            6903..7088
                     /locus_tag="VGE48_00845"
     CDS             6903..7088
                     /locus_tag="VGE48_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041959.1"
                     /translation="MHLSDDHPHTTADRICSTAEPLLGPYQSADDLEIRQHDRQVRQD
                     IVRARIDDLAERPCVGR"
     gene            complement(7138..7608)
                     /gene="greA"
                     /locus_tag="VGE48_00850"
     CDS             complement(7138..7608)
                     /gene="greA"
                     /locus_tag="VGE48_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY1041960.1"
                     /translation="MTEQETVVSQEKFDEMIKELEHLKTVRRTEIAENLEYARSLGDL
                     SENAEYQEARELQAATEERIRKLEELVKRAKIVTDGKKKDVVGFGSTVTIKKDGAGDA
                     HEYIIVGSEEADMRVHKLSHVSPLGAALMGKKKGDVFTFETPNGKQTYTVEKVA"
BASE COUNT         1670 a         2187 c         2097 g         1676 t
ORIGIN      
        1 gaggagagaa tcataaatcg gatttacaca gagtgccggc ttgaagaagc tgctgcacac
       61 gctcttgcga tggaccctga aattcccatc gttactgaaa ttccgatcct ggagtgagaa
      121 gacccggcgg ccagaaatgg ccgccattct tcatgaagac acagctttca ttttgttgca
      181 taagtgctat tctcggcttg gggtttggtg atggtgactt attcattctt gagaaatgag
      241 gagttaccac aatgtgttcc catgcgttcg ttgccctgcc gaatccgggc tttctcggcc
      301 aggacggcaa ggtcgtaacc gtaaagggct tcatccgggc gtctgacgtg gatgaagtcc
      361 gcctcctgct ctttgcaaag gactgcacgc aggacaagat cgatcgagaa ggcccttggg
      421 tcgtcatcga gcgatcggtc cgcgtggctt cgtatgacgg gatccgtcgg cacggcgcca
      481 accttgccgc cgacgtgagc aaatacttcg ctcccgacga agtcactatg cactaggagg
      541 tgcgcatgaa cagaatcgcc ctggacgcag aatgcgtcgg gcggtttttc atttgatacg
      601 atgattgtaa tgagcattcc gtatgtgctt cttgataaaa aagttggcca aacgcccctt
      661 gcggcgatag acgcttggcg agaagctaac cctgcatacg ttactgtttc tgcgacctac
      721 gcaggcaggc tcgatccaat ggctgaaggc aaactcctcg ttctacttgg agacgagtgc
      781 aaaaaccagg aacggtatag agatcttgat aaggagtatg agatcgaggt gttgcttgat
      841 ctttcaacgg acaccggcga cgggctcggt atccctatgt acggagggac cgaaactgtt
      901 ccagaaactc acgcgcgtgc aaaagctctt cgcgcacaga ccggaactca ttcagtccct
      961 tatcctgtat tctcttcaaa gaccgtggca ggcaaaccgc ttttccagta ttcgcttgaa
     1021 ggcgcgctag attcaattga agtcccttcg cataaagaga ctatctatag gatctctgag
     1081 ctcggatacg cgaaactctc caaggaagca ctaaaggaac ggatacagac aatgctttcc
     1141 ctcgcgccgc gtgttgatgc gccctcaaag agacttggcg atgacttccg ccaagaccgg
     1201 atacgcgctg cttggaacga tctgttcgat gtaatccctg atcgctcgtt tttcgtccta
     1261 cgccttcgta ttatttgcgc ttcaggaacc tacatgcgaa ccctcgcatc tcgcatcgga
     1321 aatgcgctcg gaacaacggg tatggcgcta tcgatacgaa ggacacgaat agggaaattc
     1381 tcgagactgt tcaggatctg gacccgagag tttcgatagc acgaaagtcc tgctatgctc
     1441 gtacccagag ccagaaggag ggcgtcatgg ctgaacacga aagggacagg tccctaagcg
     1501 aaccccggat ccggaaagcg gcgcttcttg taagcgtgtg cgtcgcagcc gtgagggatg
     1561 gaaaacccat cccggagcac tgcgaagtca tgggatttga ggtcgaagaa gacggcgaca
     1621 tcaggttcac cagttctgta aacatgggag ccgtcatcca agacttcgag gagccgccac
     1681 cgtaggcggc tcacttcgtt atatgacgca aaaaccgccc aatgggcggt ttttgctcgt
     1741 gtgacctcac ggggaatcga accccgattt gatccgtgag agggatctgt cctagccgtt
     1801 agacgatgag gccggtcagg acttttatac cagaaagccg cgtagggcgc caggcctagc
     1861 ggcgctgcca ctcgcgctcc tgacgtacca gctttgcgcg atccgcctcg tcatgaagga
     1921 gcgcggcggc gacccgttcg gtgcggatgc cctgagaacc tttcacgaga acgacgtctc
     1981 ccttccccac cactcctgca agcgcctcag cggcgtcctt ggaggaatcg aaggaaagca
     2041 ccttatcttg gggaagcccc gcatcacgcg cggcttctgc gatagcgcga gcccggatac
     2101 ccaccgccac gactacatct gcgccggtag cggcaagggt acctacacgt cgatgctctt
     2161 ctacggagta ccgtccaagc tcaagcatgt cgccaaggac cgcaatccgg cgggatcgac
     2221 caggaatgag agagagcgca gaaagggcct cttccacggc tgcaggcgaa gcgttgtagg
     2281 agtcgtcgat gataagagcg ccatccttcc cgcgaaggat ccgaccccgt cctgccggag
     2341 atacgtagct gcttgcgccc tgaagcgcat cttcaggaga cataccaagg gcaagcgcgc
     2401 acgcgatgcc cgctgcaggc gcgtagagct gcgagcggcc aagcgcatct tttactcgga
     2461 gcgtgaagga ctttccttct atctcgagcg ctgcttccat gccgcagtcc tccccttctt
     2521 cataaaacga gtagccggat aggtgcacgg ttgcaccgtc tgcgaatccg aacgtcgaaa
     2581 cttttgctgg gagtcggcca ctcatggata ccgcatacgg atcgtctgca gagatgatga
     2641 gtggttctcc aggagtgagt gcgtaggcag gtgcaaattc ctcttctcgt accgcctgcg
     2701 ggctcgcgta cgcttccacg tgcaccggca cctcaggaag tctcgtaacc acaaccgcgt
     2761 ctggatccgc aaagcgcaga atctttgcaa gatcccctgg cctatctgca ccaacctcaa
     2821 gcacaagaag ttttgggtaa tggtttggaa ggagaataag cgcaagtgct tcctggaaca
     2881 cacggatcca tgcgattgga ttctcccaag gattcttcgc accgattatg gtgagtggta
     2941 ccccgaactc gctgttgtag ctcttctcac ttgcgcgaag gttaaaccgg ttcgaaagga
     3001 ctgcggcggt cgcgtccttg gtcgaggtct tacctacgct tcccgtgacc atcaccacca
     3061 aggggcggta cttctttagg atggcccttg caagggtagc gagataccag gccacgagca
     3121 tctttacgag tgctttcata attacgaagg atagtctgcc cggtcaggcg ggatctcgta
     3181 ataattaatc aggaaatgtg tgagctccat gaaggggtgc gtgagggtct cggaagcgta
     3241 ctgtacccct cctggctccc tcgtgtacag caggatgatg aaccgtggct cataggccgg
     3301 gaaatatcca aagaacgaat ggaagtactt cccttcggca tatttaccgc caggacccgc
     3361 tatctgcgcg gtaccggtct tcgcggcgac actcatttca gggatcttta cagtcccgcc
     3421 agcaagcttt gagtccacca cctcaacgag catgcgcgtg gtatcttccg cagccttcga
     3481 gctgaacacg cgctccggca tgccccagga gagctgcttc tcgatccctg actcaaggcg
     3541 cactttcgat acaaggtgcg gcgtgacgat ctgcccgtcg ttagcaagtg cgcccagcgc
     3601 gcgaatcata gcgataggcg tctgcgcgat gccttgtcca taagacgcgg tggcgtattc
     3661 gatatcacgt ggactggtct caatgtttgc gaggtctcca cgaatctcgc tcgggagatc
     3721 gattccggtc tccgtattca taccaaggtc cttgaagtac cctcggaagc gctcatgccc
     3781 tacctgaagt gcaatgtatg cggcacccat gttaagagac tgcgagagca cttcctgcat
     3841 cggggttccc ccaccgcgcg ccttaagatc gtagttgcag aatttcttgt tgttaagcgt
     3901 ggtgcagcct gtgtcgttat aggtcgaggt cggtcccacg acaccagaat caagtcctgc
     3961 tgccatggtg agtgccttta cgatggagcc gaactcgtac cggtgctcta ccaacgggtt
     4021 cccgaagtgc gtggagtccc cattctggaa gtcattcagg ttaaatgacg gagcggtatc
     4081 aagcgcgatt atcgcccctg tcttcgggtc catgatgatg ccgccggtct cgcgacttcc
     4141 atactgcgca tttatagaaa gcaatacctg atcaagtttc tcctgcacca tcggctcgat
     4201 actggtcacc agatctccct cacgcgcgcc acggccgtct gcgactgcgt tgtcgatgtt
     4261 cgcaaacagt tctgcgaaga agttcccaaa cacgccggtt gagccacgtg aaagtacgtc
     4321 ttcgtagtag cgctcaagcc caaagcgccc cgcgatcgtg tcgtcgtcat cgaacgccac
     4381 gaacccaatg gactgtgctg cgcgggtctc gcctgggtac gagcgccagc gctctcgcga
     4441 cacagagatt cctggcaagt cgagagcctc gatggcacga ccttcgtctt cgggaacgcg
     4501 cttcgcgaca acctcatacg ggtcctcctt gcgcgcgacc gactggaaga acggttcgcg
     4561 ctcaatcggg aagatcgcgt ttatggccgc gtaggcagct tcaggatctt cgacgagctt
     4621 tgggctcatg gcaaccgtaa aacctgtggc caaggttgca gccgaaagca cgcttccatc
     4681 ctttcgtgtg aagtatatgg aaccacgatc atacagctcc tggctcgcgg acatgtactg
     4741 gcgctctgca cgcagaccaa attccccacc atgtactact tgtacaaagt agagacgtgt
     4801 aaccaaaagg agcgctacgg ctgcgagccc gaaggtgaga agcctgagtc tgagccgtat
     4861 cgccctgcgc atagccttag cggatggaga cggtcggtgc agcctgcgtc gtcgcgtagg
     4921 ttacgctccg cggcttctcg aggccacgac ttcctggatc tgcgcgatct atgtctgcca
     4981 ccatggcata gaaacggctt tcaaggcgcg cgatgtcctg ctcagtctcg tgcacctcga
     5041 tggaaagact cgtcttccac gctgccatgg agaccgtaac tgcgacgagc atgaggtacg
     5101 ctgcgacgag cgcggctgcg gtgtacccga ggtatggaat ggtcttgcta ggcataagcg
     5161 gggaccgagg ttaggttgat gcgtttttta agttctctgg gaccagaaac gaacgtgcgg
     5221 agtttcgcgg atctggcccg gggattccgt acggtctctt gaaccgacgg ggctatgggt
     5281 tttctcgtaa gcaggcttcc cttccctgct tctaccgcct cgcggaacat agtctttacg
     5341 atgcggtcct caatgctatg gaaggttatg accgcaacac gaccaccggc cgggacgcgt
     5401 tccattgcag ctgaaagtcc ctcacggagt gctccgagtt cgtcgttcac tgctatgcga
     5461 agcgcttgga aggtcttcgt tgcaggatgg atgcggcgat gacggtacca ggaaggaacc
     5521 ccttgctcga tagccgccac aaggtcattt gtggtttcaa gcgaaccgtt ctctcgtgcc
     5581 gcaacaatgg cgcgtgcgat agaacgcgcg aagcgctcct cgccaagaga ccacaacatg
     5641 tccgctagcg acgcttccga aaggtggttc acgaggtcag cagccgtagt gccctcagcg
     5701 gggtctccat aggtcatatg aagcggctca tccaccttga aggagaatcc acgaccacgc
     5761 gtgagatgga agccactcca tccgagatca aagagcactt tgtctggggt catacccgct
     5821 tcgtcgagca tcgttgcaag ctcacggaag ttgccgtgca tcaaagagac attcggacgc
     5881 tcgctttctg gaacctccgc cactaccttg ctggcgcgcg tgatggcgtc ttcatccgca
     5941 tcaatgccca taagcgcgcc gtccttaccg agcttttgaa gaatgagcgc gaaatgccct
     6001 gctcccccca gagttgcgtc cacgactgca tctgacgcct gtatatcgag tgcatcgacg
     6061 gcttcttcaa ggagaacggt gtcgtggcgg ccttccatat gctattggag tgcctttgca
     6121 ccgagtgcct cagcgaacga gtccgcatca cgctcaatgt ccttggtgta cgcttcccac
     6181 gctgcttcgt cccaaagctc gacacgatcg gagacacccg caactacaca cttccctgaa
     6241 agggtggcgt aagacttcag ataatccggc acgaggatgc ggccggcgct gtcgacgtct
     6301 gcttcggaag cgcctgcgag catgaggcgc gcaaatgcac ggccagctgc agtacctgag
     6361 gagtgtgccg aaaacttctg tgcttccttt ttccaggcgg ccttcggata tacgaagaga
     6421 cagtgatcaa ggccgcgcgt cacaatgacg gatgcaccga gctccttgcg gaacttcgcc
     6481 ggcaacgaaa gccggttctt tgcatcaagc gtgtggcggt attctccgat gagcatggaa
     6541 gtttcggcgg aggataaaag gagcgtactc ccacttcatc ccacgtatac acaagtatat
     6601 gacactacgc cccacttccc ttcatgcttc atacagaaag ttgtgtataa ccagggtttg
     6661 tttgaatcag gcgttcaggc tatgctcata cagcaatgac tcaagcgatc tgcaacttcg
     6721 tgtttaccac cctcgtctac gtcggactca ttcgtccggt cggggtttcg cttgaggttt
     6781 ctagagaaaa gtagacaaag aaacttgaaa cgagcccccg accggaaacg gacgggggct
     6841 cgttcgctag aacggcgaca caaagaagtc ctttgttaac cgcatggaaa aggaggcaag
     6901 ccatgcacct ctcagacgat cacccgcaca cgaccgcaga tcgcatctgc agcaccgccg
     6961 aacccctact cggaccctat cagtctgccg acgatcttga gatcaggcag cacgaccgac
     7021 aggtgcggca ggacatcgtc cgagcacgca tcgatgatct tgccgagcga ccctgcgtcg
     7081 gacggtagag gcatcgtcct caaaaccaaa aggccccgct tttgcggggc ctttcagcta
     7141 tgcaaccttc tctacagtat aggtctgctt cccgttgggt gtttcgaagg tgaagacgtc
     7201 tcccttcttc ttgcccatga gcgcagcgcc gagcggagag acatgcgaga gcttgtgcac
     7261 acgcatgtcc gcctcttcgg aacctacgat gatgtactcg tgcgcatcgc ctgcaccatc
     7321 cttcttgatg gtgacggtcg agccgaatcc gacgacatcc ttcttcttgc cgtccgttac
     7381 gatcttcgcg cgcttcacga gctcctcgag cttcctgatg cgctcctcag ttgcggcctg
     7441 caactctctc gcctcctggt attcagcgtt ctcagaaagg tccccaagtg agcgagcgta
     7501 ctcgaggttc tccgcgatct cggtacgacg aaccgtctta aggtgctcga gctccttaat
     7561 catttcgtcg aacttttcct ggcttacgac agtctcctgc tcggtcatgg gtgtaatgaa
     7621 tgaaatatga
//
LOCUS       DASWXU010000026         8313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_887216, whole genome shotgun sequence.
ACCESSION   DASWXU010000026 DASWXU010000000
VERSION     DASWXU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887216"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGE48_00855"
     CDS             complement(<1..339)
                     /locus_tag="VGE48_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041961.1"
                     /translation="MRIFATIFRYLLPVALVGLVAHYALSDIKDPPRCTEPIAYTVLA
                     PDPRFELSQEAFITAAEEAALVWNEAAGKELFRRGDVGIAVSLVYDERQEALALGDSI
                     EAGQQEYRAMK"
     gene            complement(343..1371)
                     /gene="dnaX"
                     /locus_tag="VGE48_00860"
     CDS             complement(343..1371)
                     /gene="dnaX"
                     /locus_tag="VGE48_00860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY1041962.1"
                     /translation="MAHQSLYRAYRPQSFKDVAGQDQVVKPLEEQIASGKVGHAYLFA
                     GSRGLGKTSIARIFARELKTDERDIFELDAASNNGVDEVRALNESVHTLPYNSERKVY
                     ILDEAHMLSKSAWNALLKTLEEPPTHVVLILATTELEKVPETVRSRCQVFQFKKPVRA
                     GLAALVASVAKKEGYTIEPAAADLIAHLGDGSYRDALSVLEKALASATDKKLSREDAE
                     KATGAPRHALVHALAAALSAGSAENALQTIRTASAQGVDIPLYLSLLLEYLRQVLLMR
                     HASELRKEISEELGEDAYQEALTLAEAKDSKLTHETLRAFLEASARVRFAPVPELPLE
                     LVVLEHSS"
     gene            complement(1384..2349)
                     /locus_tag="VGE48_00865"
     CDS             complement(1384..2349)
                     /locus_tag="VGE48_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY1041963.1"
                     /translation="MESFWNTLPKPFFVLAPMADVTDPAYRSLIASAGKPDVTWTEFV
                     SADGLYHMRDKKGIPDDENPLMRDLQFTSEERPIVAQLFSSNPESMAYAAQLCAELGY
                     DGIDINMGCPDKSIEKQGAGAAMIKNPEKAKEIIRAARTAGIPVSVKTRIGYNQESID
                     EWLPNLLEENLPALTVHLRTRKEMSKVPAHWDLMPHIVALRDRIAPDTKIIGNGDVET
                     IAEARQKAHDTGCDGVMIGRGIFGNPWVFAGRAKEDTPRDDKLAALLSFARDFEKLQP
                     AKSFHLVRKHVKAFTAGFEGSAQLRAALMETQSADDMERVIEEAA"
     gene            2451..2849
                     /locus_tag="VGE48_00870"
     CDS             2451..2849
                     /locus_tag="VGE48_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041964.1"
                     /translation="MDRTVSTVIRTGHGIVVVRSRKPKENQWRLPKVPIAADADWREE
                     NSIFIRKTTGLRIPKGRFLALREDNESILCTAAVSDMDVIRDIISLGEGGIEVHEVSY
                     YLVNNGSSVQKPVRVLLQQQKLNRPPEAGT"
     gene            complement(2870..3793)
                     /locus_tag="VGE48_00875"
     CDS             complement(2870..3793)
                     /locus_tag="VGE48_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041965.1"
                     /translation="MDMRAPARKPAAAPAPVTVPASKKNVTISRKKTAKAEPKEPLKK
                     RRKKAKKALFVALGIVTVLFLALLVYLSWLPALRVSAVQADGPHAEEVKQIVETGLQG
                     THAFVLPRNSLFFIPEEDIRARILDAHPDIEAVSIRAEGLTTLSVSTTARAEAYVWCG
                     ISTEVTFGTCFSANAEGLIFAPVPPEVASTSAALRIYAPIVGQQGESPVRARIESADR
                     IPGVLLFVKAMRTLGADVTALAFRADEADLYTEMGTRITYVLGREEQAAGIAASAFPQ
                     LSLNDGSIQYVDLRFPGKAYFKRRGEVQIEE"
     gene            complement(3849..5117)
                     /gene="serS"
                     /locus_tag="VGE48_00880"
     CDS             complement(3849..5117)
                     /gene="serS"
                     /locus_tag="VGE48_00880"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY1041966.1"
                     /translation="MLDIHFIRENADLIKAGAQKKHIEVDIDRLLQADDERKRLRQEL
                     DSRRAEQNRASQTIALAKGEEKEKLLEAMRHVKEGMAEIEEKYKAVMEEWQTLMLSVP
                     NLPDMTVPEGESDADNVEVKQWGERPTFAFAPKDHVELMSRNGMADFERGAKVAGFRG
                     YFLKGDGVLLANAVSNLALAHFSGKGFTPMIVPSLVRRETFMGTGYLPQGEEDLYKTQ
                     DGEYLAGTGEVATMGYYMDETLEKSQLPAKFLSYSPCFRREAGSHSKDTKGLIRVHEF
                     FKWEQVVLCEASHETSVTLHEELTRNAEEFLEKLGLPYRVVVNCGGDLGLGQVKKYDI
                     EAWMPSQDTYRETHSSSYFHDFQTRRLNLRYRDVDGSLKFAHSLNNTAVAMPRVLTMI
                     VENFQQEDGTVRVPDALVPYVGKEVLGHSA"
     gene            5205..6068
                     /locus_tag="VGE48_00885"
     CDS             5205..6068
                     /locus_tag="VGE48_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY1041967.1"
                     /translation="MADIEEILKEEYEERGIGSLLEMPQPPERLWLRGSLPGSEVKRL
                     AVVGSRALSPYGEAACRYLISGLAGYPISIVSGLALGADACAHRAALEAGLHTIAVPG
                     SGLANKTIAPRTNYALAMYILASGGALLAEHPPEEGARPYYFPSRNRIMVGLTDAVLV
                     IEAAEKSGTLITARMAADYNRDLLCIPHRILDENGQGNHRFLRLGAALVTEPEHILEA
                     LGVTSEEKQQPRPLLSPAEEQLYEVLATPLPRSELIRRSGLLPDEALVALLALDLKGL
                     VKETYGAWRRV"
     gene            6132..>8313
                     /gene="topA"
                     /locus_tag="VGE48_00890"
     CDS             6132..>8313
                     /gene="topA"
                     /locus_tag="VGE48_00890"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY1041968.1"
                     /translation="MAKRLLIVESPAKARTIQQYLGKEYEVLASVGHVRDLPKSNKDA
                     VDIEDGFVPKYVISPDKREVIEKIAKAAEKAEEIYLATDPDREGEAIAWHVKEAAKLK
                     KPQRVVFHEITKTAVEEALAHPRAIDEHLRRAQEARRVLDRLVGYDLSGLIWKKVRYG
                     LSAGRVQSPALRILAEREREIQAFVPETYYTLDAVFKAKTEFPASCVEEPTDPKEAER
                     IVNEGRSASWTVAEVKERSEERQPRPPFTTSTLQQAASTRLGFSPSRTMRAAQKLYEA
                     GHISYMRTDSVNLGTDAKAALARAVEKEFGKDYVEVRAYQTKSKNAQEAHEAVRPTDP
                     SRETAGATGDEQALYTLIRIRAMASQMASAKLQRTSVKTKANCDIPLFSATGSRVTFP
                     GWLALDTRARGEDVELPKLTEGEPLTLISLGSLEKQTEPPNRYTEAGLVKELEKRGIG
                     RPSTYASIMKTIQDRGYVEKTGRTLTPTATGMVVSGWLEEHFADYISDSFTAEMEDEL
                     DEIARGERGYEATLSEFYGPFSKAVKAKDSLPKATSLGAVPEEFTCPLCGSPMEFKLG
                     RGGVFMSCTRYPECEGARQEDGTELKGDEPIGNDPTTGAPIFVKTGRYGPYVEMELPE
                     EEKPVELTKAGKPKKSRGRPKKALKRASIPPGTDLSSVTLADALHYLSLPRELGNHPS
                     TGKPVMANVGRFGPYVGHDGEFRSLKGADDPYTITLDRAIEILAE"
BASE COUNT         1768 a         2481 c         2336 g         1728 t
ORIGIN      
        1 cttcatggcg cggtactcct gctgccccgc ttcgatcgaa tccccgagcg cgagcgcttc
       61 ctgtcgttcg tcgtatacga gcgacacggc tataccgaca tcgcccctac gaaacaactc
      121 cttgcctgcc gcttcattcc agaccaaagc cgcctcttcc gctgcagtta taaacgcctc
      181 ctgagagagc tcgaaacgag ggtcaggagc aagtactgtg tatgcgatag gctcggtaca
      241 gcgcggcgga tccttaatgt ctgagagcgc gtagtgcgcg accagaccga caagcgccac
      301 cggaagtagg tagcggaata tggtcgcaaa tattcgcata cgctaggaag agtgctcaag
      361 caccacaagc tcaagcggaa gctcaggcac cggtgcaaac cgaacacgag cagacgcttc
      421 aaggaacgcg cgaagcgtct cgtgcgtgag cttcgagtct tttgcctcag caagcgtgag
      481 cgcttcctga tacgcatctt ccccaagttc ctccgagatc tctttgcgaa gctcagacgc
      541 gtgacgcatg agaagcacct ggcgaagata ctcgagcagg agtgaaaggt agagtgggat
      601 atctacgccc tgcgcagacg cggttcgaat cgtctggagc gcattttccg cgcttcctgc
      661 agagagcgct gcagcgagcg catgcacgag cgcatgtcgc ggagcgccgg ttgctttctc
      721 cgcatcctca cgcgaaagct tcttatcagt tgcagatgcg agcgccttct caagcacgga
      781 gagtgcatca cggtatgacc cgtctcctag gtgtgcgata aggtctgcgg cagccggctc
      841 tatggtgtac ccctccttct ttgcaactga tgcgacaagc gccgcgagtc ctgcgcgaac
      901 cggcttcttg aattggaata cctggcaacg agaacgtacg gtttccggaa ccttctcaag
      961 ctcagtggta gcgagaataa gtacaacgtg cgttggtggt tcctcaagcg tctttagaag
     1021 cgcattccat gcggacttcg agagcatgtg cgcctcgtcc aggatgtaga ccttccgttc
     1081 tgagttgtac gggagcgtat gcacgctctc gttgagcgca cgtacttcgt ctacaccatt
     1141 attagacgct gcatccagct caaagatgtc gcgttcgtcg gtcttgagct cgcgcgcgaa
     1201 gatgcgtgcg atggaagtct ttccgaggcc acgggatcct gcaaagagat atgcatgccc
     1261 caccttcccg cttgcaatct gctcttcaag cggctttacc acctgatcct gccccgcgac
     1321 atccttgaaa gactgtggcc tataagccct atagagcgat tggtgcgcca tgcgggaagt
     1381 atactacgct gcttcctcga taacgcgctc catatcatca gcactctgcg tctccatgag
     1441 tgcggctctt agctgcgcgg aaccctcgaa tcctgccgtg aacgccttca cgtgtttccg
     1501 cacgaggtgg aatgatttgg caggctgcaa tttctcgaag tcacgcgcaa acgagaggag
     1561 cgccgcgagc ttatcgtcgc gcggagtatc ttccttcgcg cgtcctgcga atacccacgg
     1621 attcccgaag atgcctcgcc caatcatcac gccgtcacag cccgtgtcat gcgctttctg
     1681 acgcgcttca gctatcgtct cgacgtcacc attgccgata atcttcgtat ctggggcgat
     1741 gcgatccctg agcgccacga tatgcggcat gaggtcccaa tgtgccggaa ccttcgacat
     1801 ttccttacgt gtacgtaagt ggaccgtgag cgcaggaaga ttctcctcga gcagattcgg
     1861 aagccattcg tcgatggatt cctggttgta gccgatgcgt gtcttcacag acaccgggat
     1921 tccagcggtg cgggcggcac ggattatctc ctttgctttc tcagggttct tgatcatcgc
     1981 cgcgccggcg ccctgcttct cgatagactt gtccgggcag cccatgttga tatcaatacc
     2041 gtcgtagccg agctccgcgc agagctgcgc ggcataggcc attgattctg gattgctcga
     2101 gaagagctgc gcgacgatcg ggcgctcttc gcttgtaaac tggagatcgc gcattagcgg
     2161 gttctcgtcg tctggaatgc ccttcttgtc acgcatgtga tagagcccgt cagcagacac
     2221 gaactctgtc caggtaacgt ccggcttccc tgcggacgcg atgagcgaac gatacgctgg
     2281 gtcggtcacg tccgccatag gcgcaaggac gaagaagggc ttcgggagcg tattccagaa
     2341 cgattccata gcggcatcct accggctata tgcatattcg acaatgcgga ccgcttcgcg
     2401 taaagtgcga gcggacgcgc ctggttaggc gcagaaacgg agaagtggcc atggacagaa
     2461 cggttagcac cgtcatccgc accggccacg gaatcgtggt cgtgcgcagc aggaaaccca
     2521 aagagaatca gtggaggctg cctaaagtac cgatagcggc cgacgccgac tggcgggaag
     2581 agaattcgat ttttattcgg aagactaccg gacttaggat acccaaaggt aggtttcttg
     2641 cccttaggga agataacgaa agtatcctct gcaccgcggc cgtgtctgac atggatgtga
     2701 taagagacat catctcgctc ggagagggcg gaatcgaagt gcacgaagtc tcgtactatc
     2761 tggttaacaa cggctcatcg gtgcaaaaac cggttcgggt tctgcttcaa caacaaaaac
     2821 tcaacagacc ccctgaggcc ggcacatgat tgtatgcgcc ggccttttgc tattcctcta
     2881 tctgtacttc ccctctcctt ttgaaatacg ctttccccgg gaacctgagg tccacgtact
     2941 gtatggatcc gtcattcagg gagagctgcg ggaacgcact tgccgcgatg ccggctgcct
     3001 gttcttctcg cccgagcacg tatgtgatgc gggtgcccat ctccgtatac agatccgctt
     3061 cgtccgcacg gaacgcaagc gccgtaacat cggcgccgag cgtgcgcatc gccttcacaa
     3121 aaagaagtac gcccggaatg cggtccgcgc tctcgatgcg tgcgcgtacc ggcgactctc
     3181 cctgctgccc tacgattggt gcataaatgc ggagcgctgc cgaggtcgac gctacctctg
     3241 gagggaccgg cgcgaatata agtccttctg cgttcgcact gaagcaagtt ccgaatgtga
     3301 cttcggttga gatgccgcac cacacatagg cttcggcacg agcggtcgta gacaccgaaa
     3361 gcgtcgtaag tccttcggca cggatcgata ccgcctcgat atccggatgt gcatcaagaa
     3421 tgcgcgcacg gatatcctct tccgggataa agaagagcga attgcgcgga agcacgaaag
     3481 cgtgcgttcc ctgaagaccc gtttcaacaa tctgcttaac ctcttctgca tgcgggccgt
     3541 ctgcttgtac cgcagacacc cgaagcgctg gcagccacga gagatacacc aggagcgcga
     3601 ggaaaagtac cgtgacgata ccaagcgcta caaagagcgc cttcttcgct ttcttgcgcc
     3661 gcttcttgag tggctctttc ggttcggcct tcgccgtctt cttgcgagag atagtgacgt
     3721 tcttcttcga ggccggaacg gttacaggag caggcgctgc agcaggcttt ctggcaggcg
     3781 cacgcatgtc catagagcgc ttcgggcgcg ctacgggcgt gagcgcagga gtctgcgctt
     3841 tcctcgcctt aggctgaatg tcccaggacc tcctttccga catacggtac gagcgcgtcc
     3901 gggacgcgaa cagtgccatc ctcctgctgg aaattctcca cgatcatcgt gaggacgcgc
     3961 ggcatagcaa ccgcggtgtt attcaaggag tgagcgaact tgagcgaccc atccacatcg
     4021 cggtatcgga ggttcagtcg acgcgtctgg aaatcatgga aataggaaga agagtgcgtc
     4081 tcgcgatacg tgtcctgaga cggcatccac gcctcgatgt cgtacttctt cacctgaccg
     4141 aggccgaggt ctccgccgca gtttacgacg acgcggtacg ggagcccgag cttctcgaga
     4201 aattcctcgg cgttacgcgt gagctcctca tggagcgtca ctgacgtctc atgcgacgcc
     4261 tcgcacagca cgacctgctc ccacttgaag aactcatgga cgcggatgag tcccttcgta
     4321 tccttgctgt ggcttcccgc ctcacgacgg aagcatggcg aatatgagag gaattttgcg
     4381 ggaagctggc tcttctccaa agtctcgtcc atgtagtacc ccatggtagc cacctctccc
     4441 gttcccgcga gatactcgcc gtcttgggtc ttgtagagat cttcctcacc ctgcggcagg
     4501 tatccggttc ccatgaaggt ctcacggcgc accaaagacg gtacgatcat cggcgtgaag
     4561 ccctttccag agaaatgcgc gagcgcgagg ttcgataccg cgttcgcaag cagaacgcca
     4621 tcacctttca aaaagtaccc gcggaacccg gcgacctttg cgccgcgctc aaagtccgcc
     4681 atgccatttc tgctcatgag ctcgacatgg tccttcggtg caaacgcgaa cgtcgggcgc
     4741 tcgccccact gcttcacctc gacattatcc gcgtcgctct ctccttcagg aaccgtcata
     4801 tcaggaaggt tcggtacaga gagcatgagt gtctgccact cttccataac cgccttgtac
     4861 ttctcctcga tttcggccat gccttctttc acatgacgca tcgcctcgag gagcttctcc
     4921 ttctcctcgc ccttagcgag cgctatggtc tggctcgcgc ggttctgctc ggcgcgtcgg
     4981 ctatcgagct cctgacgtag gcgcttccgt tcgtcatcag cctgaaggag gcgatcaata
     5041 tcgacctcga tatgcttttt ctgggcgccc gccttgatga ggtccgcatt ctcgcggata
     5101 aaatggatat cgagcatgtt cccgccattg tacggcagga ccgcgcctac gaaaagcgga
     5161 ggattttata ttttctttat ctcccttctg tatgcttcct gcatatggcg gacattgagg
     5221 agatcctgaa agaggaatat gaggaacgag gcatcggatc gctccttgaa atgccgcaac
     5281 ctccggagag gctctggctg cgtggctcgc ttcctggttc tgaagtaaaa cggcttgcgg
     5341 tagtgggatc gagagcgctc tcaccctacg gcgaagctgc atgccggtat ctcatatccg
     5401 gactcgcggg ctacccgatc tcgattgtct cggggctcgc gctgggtgct gatgcgtgcg
     5461 cgcaccgggc tgcgctcgaa gcggggctcc acacgatcgc cgttcccggc tcaggactcg
     5521 caaacaaaac tatcgcgccg cgaacgaact acgcacttgc gatgtacatc ctcgcatcag
     5581 gcggtgcgct ccttgcggag cacccgcctg aagagggtgc tcgtccgtat tacttcccct
     5641 cgcgcaatcg gatcatggtg gggctcacgg atgcggtgct ggtgatcgaa gcggcagaga
     5701 agtctggcac gctcataacg gcacgcatgg ccgctgacta taaccgcgac cttctctgta
     5761 tcccacatcg catccttgat gagaatggcc agggcaatca tcgttttctg cgtcttggtg
     5821 ccgcgctcgt aacggaacca gagcatattc ttgaagcgct tggggttact tctgaggaaa
     5881 agcagcagcc gcgcccgctg ctttctcctg ctgaagaaca actctacgaa gtactcgcta
     5941 cgccgctccc acggagcgaa ctcatacgcc gctcaggact tcttccagac gaggcactcg
     6001 tagcgctttt agcgcttgat ctaaagggtc tggtgaagga aacgtacggt gcgtggcgca
     6061 gggtttagag ccttcacgca gcctccacgc ttgaccatat ataggtatgc gcggtatctg
     6121 tgggtacaca catggctaaa cggcttctta tcgtagagtc gcctgcaaag gcgcgcacca
     6181 tccagcagta cctcggcaag gagtacgagg tacttgcatc cgtgggccac gtccgcgatc
     6241 tcccgaagtc caacaaggac gccgtagata tcgaggacgg cttcgtgccg aagtacgtca
     6301 tatcccctga caagcgcgag gttatcgaga agatcgcgaa ggccgcagag aaggccgagg
     6361 agatatatct tgcgaccgac cccgaccgtg aaggcgaggc tatcgcctgg catgtgaagg
     6421 aagccgcgaa actgaagaag cctcagcgcg tggtcttcca tgagatcacg aagactgctg
     6481 tcgaagaagc gctcgcacac ccgcgcgcta tcgacgagca ccttcgccgt gcgcaggaag
     6541 cgcgccgcgt ccttgaccgt ctcgtgggct acgacctctc cggcctcatc tggaagaagg
     6601 tgcgctacgg actttctgca ggacgcgtgc agtcgccggc cttgcgcatc cttgctgaac
     6661 gcgagcgcga gatccaggcg ttcgtccctg agacgtacta cacacttgat gcagtattca
     6721 aagcgaagac cgagttcccc gcttcgtgcg tcgaagaacc aactgacccg aaggaagcag
     6781 aacgtatcgt gaacgaagga cgctccgcgt cctggaccgt tgccgaagta aaggagcgct
     6841 ccgaagagcg ccagccgcgt ccgccgttca ccacctctac cctccaacag gcagcctcca
     6901 cgcgcctcgg attttcccca agccgtacca tgcgcgccgc gcagaaactg tatgaagcgg
     6961 ggcatatctc gtacatgcgt accgactccg tgaatctcgg aactgatgcg aaggcagcgc
     7021 ttgcacgtgc tgtagaaaaa gagtttggta aggactacgt tgaggtgcgc gcgtaccaaa
     7081 ccaagagcaa gaacgcacaa gaagcacacg aggcagtgcg ccctacggac ccgagccgcg
     7141 agaccgcggg cgcaaccggc gacgagcagg ccctgtacac cctcatccgt atccgcgcga
     7201 tggcgagcca gatggcttct gcgaagctac aacgcacgag cgtgaagacg aaagccaact
     7261 gcgacatccc tcttttctct gctaccggtt cccgcgtcac cttccccggt tggcttgcgc
     7321 ttgatacacg ggcacgcggc gaggatgtcg aacttccgaa gcttacggag ggcgaacctc
     7381 tcacactcat ctctctcggg tctctcgaga agcagaccga gccgccaaac cgctacacag
     7441 aagcgggact tgtgaaggaa ctcgagaagc gcggcatcgg acgcccatct acctacgcat
     7501 ccatcatgaa gaccatccag gatcgtggct acgtagagaa gaccggccgt actcttactc
     7561 cgaccgcaac cggcatggtt gtctccggat ggcttgagga acatttcgca gactacatct
     7621 ccgactcgtt caccgcagag atggaggacg agctcgatga gattgcgcgc ggcgagcgcg
     7681 gctatgaagc tacgctctct gagttctacg gcccgttctc gaaagccgta aaggcgaaag
     7741 attctctccc gaaggcgacg tcgcttggcg cagtcccaga ggagttcacc tgcccgctct
     7801 gcggaagccc tatggagttc aagctcgggc gcggcggcgt gtttatgagc tgcactcgct
     7861 acccggagtg cgaaggtgcg cgtcaggaag acggcaccga gcttaagggg gacgagccta
     7921 tcggaaacga ccctaccacc ggcgccccta tcttcgttaa aactggacgc tatggtccgt
     7981 acgtcgagat ggagcttcca gaagaggaaa agccggttga actcacgaag gccggaaagc
     8041 cgaagaagag ccgcggacgc ccgaagaagg cactcaagcg cgcaagcatc ccgccaggta
     8101 ccgacctttc gtccgtgaca cttgcagacg cacttcatta tctctcgctt ccgcgcgagc
     8161 tcggcaatca tccttcgacc ggcaagccgg ttatggcgaa cgtaggtaga ttcggtccat
     8221 atgtgggtca tgacggcgag ttccgttcgc taaagggcgc agatgatcca tatacaatca
     8281 cactcgaccg tgcgattgaa attcttgcag aac
//
LOCUS       DASWXU010000027         7570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_386967, whole genome shotgun sequence.
ACCESSION   DASWXU010000027 DASWXU010000000
VERSION     DASWXU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386967"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1601)
                     /locus_tag="VGE48_00895"
     CDS             complement(222..1601)
                     /locus_tag="VGE48_00895"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY1041969.1"
                     /translation="MAVSLDQIVSLAKRRGFIFPGSEIYGGLAGTFDYGPLGVALKNN
                     IKALWWKRFVEDRDDMYGVDASLIMPARVWEASGHTETFNDPLVSDAKTGERFRLDHV
                     LEAAGIDPAGMTLEDMWSALQTAGLTTPSGNAYEQPTKFNLLFPVTMGASQGSATLAY
                     LRGETAQGMFVNFKNIIDAYHPKLPFGMGQIGKAFRNEIAPREFVFRLRELEQLEVEY
                     FVHPDAWKESFEAWRLEMQAFANDLGLPMDKLHEKEIGDEDRAHYSERTIDFEYEFPT
                     GTKELWGLAYRTDYDLSRHAEYSGVSLTYLDEEAKEKIVPHVIEPSLGLERTMLAVLA
                     SAYDEDELGGEKRVVLRLSPKVAPVKAMVSPLLKNKPELVARAKEVRAALKKAHPAIA
                     WDDNGNIGKRYRRQDEIGTPFCVTVDFDTLGETPELLGTVTLRHRDTGDQERLTVDEV
                     AERIRESLA"
     gene            complement(1614..3230)
                     /locus_tag="VGE48_00900"
     CDS             complement(1614..3230)
                     /locus_tag="VGE48_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041970.1"
                     /translation="MAAYFLVFGGRAVSTERIALTVDGQTAVGSGDVATLLISVENRN
                     PVTILNTSIRAEFPETARSPEDNTRPFVHYEDTMGDVPSGETATRSVRVVLYGGENER
                     VVVPVRFEYRVEGSNATYVKETKHEILISSSPLSIRAEAVSEASVGQPITFAVTVRSN
                     AKEPLENVAVYGQYPFGFTPARGQAAIIPVGTLAPGEERTVTVTGTLSGEDNEERVLR
                     FSAGTRTSPDANLLAVTYATAITPVTLAKPFLATSLSFNRDTSATPVLEAGAPVQGII
                     SWINTTAAGILDAQVTLRFEGNALDTNSIAGYGGFYRSSDRTIIYSRETEMGLARLDP
                     GATGSGSFSFKTKSAEALVGTRSPSVDITVSVAGRRVGEGNVPETINASLTRTLKVGT
                     SLALSGRVSHRAGPLPPIADQESRYAIVLALSNTVNTVADATVTGTLPSYVRFVSSTD
                     PSITYNETSRTVTWKAGEVASGAGFSAGARQGTFEVAVLPSASQRGTSPVLMSSLEVK
                     GVDRYTQGQIQGAGAAITTDTSGQGTGTVR"
     gene            complement(3487..4038)
                     /locus_tag="VGE48_00905"
     CDS             complement(3487..4038)
                     /locus_tag="VGE48_00905"
                     /inference="COORDINATES: protein motif:HMM:NF023393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HEY1041971.1"
                     /translation="MRHKYATQAIVLARTPLKESATLLTLLTEEFGVLRARAEGLRKP
                     GAKLAHALQTLDQCDVTLVRGKEGWRLSGAHLEERWFGKLDRRGRERAGRVNGLLLRL
                     VHGETNDTFLFSIFLRFLEALSGREEEEQDVAECFAALRMLASLGLDAGAIPEDNEYA
                     PLAPDERRELVTRINRGIAASGL"
     gene            complement(4028..7405)
                     /locus_tag="VGE48_00910"
     CDS             complement(4028..7405)
                     /locus_tag="VGE48_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1041972.1"
                     /translation="MADTEKSEVAKREEAILAFWKEKEVFEKTLSKDAPKGEFVFYDG
                     PPFATGLPHSGSLLSSVSKDLIPRYKTMRGYRVRRRWGWDTHGLPIESLVEKKLGLKN
                     KKEILEIGIEKFNETARSMVLEYVHDWKRYVERVGRFVDFDNSYKTMDNTFIESVWWG
                     LKEIHKKGRLYEGRKVLMYCPHCETPLAKAEIAMDNTYKDVTEETATVAFRVLDSKDS
                     GLPENTYLLAWTTTPWTLPGNVALAVGGDIAYGVYEKDGKHLIAAKERAEALGLTNAT
                     REFSGSALVGVTYEPLYEIPKAARHTGKKWEVLSADFVTTTDGTGIVHTAVIYGEDDY
                     QLGVKEGLPMVPLLNPNATYNDDAPLFLRGKYIKKAESEIKEDLESRGRLFAREMHTH
                     SYPHCYRCGTALIYNAVSSWFIDIQAVKERMLAENEKITWVPEHLKHGRFRHIVENAP
                     DWTISRNRFWASPLPIWKGADGSVHVIGSLAELKERTKKSGNRYLAMRHGETENTLIS
                     RLSSDPTKNPLTEKGRERVRARAQELKANPPTRIFASPVLRTRESAEIICEELGLPKD
                     KLVFDERLRELTFGEFEERYLEEFLAWRNAHAYDEAPEGGESGLEAKRRFGEFLYELE
                     RTYANETILIVTHGIGLESFTALVDGLENKEAKELIESSSFSPAEVIEVPFVPLPHNR
                     DFTLDYHLPYIDRVSLVDEAGEPFTRIPEVVDCWVESGSMPFAEYHYPFENKEAFEKR
                     TPGDFISEYIGQTRAWFYYMHAISLELFDHLAFKNVITTGNVLAADGAKLSKSKNNYT
                     DPYLLFEKYGADAFRYTLMSSVVMQAEDLTFRDDDVKDAHARVVNMLRNIYGFFEIYK
                     DELAPNARPSTDHPLDRWIQARLDALVTDVTDSFDRYDVPRGVRAYRDFIDDLSNWYV
                     RRSRDRAKGEDEGDKQRMLAMLRSVLATFSKTIAPVMPFIAEEIYQGVRKDSDPESVH
                     LASWPSVEKGIFDALSGRAKKDAILLEEMKRTRTLASEALRLRQKADLKVRQPLASLS
                     IPGVLSAELAKILAEELNVKEILMNAEEMALDTELTPELIAEGDERAFQRAVAEARKA
                     EGFSPKDRATVTKREDGAHVAELSTGPVRFSLSRDAS"
BASE COUNT         1545 a         2289 c         2105 g         1631 t
ORIGIN      
        1 gacgcgtgaa gaaggttacg cccggctcga tcgcggacgc gataacgatc gccgtgagca
       61 cgagaagcgc gatgtcccga aggagccaga gcgcgtaggc tgccgctcct atgaggagcg
      121 tcatgatgat cgtgcccggg gtgatggtga tggagacgtt cttttgcatg aggaaaatgg
      181 taccacgaaa cgagaaagcg gcagccctgc tgccgctttt cctaggcgag cgactctcgg
      241 atgcgctctg ctacctcatc gaccgtaagg cgctcttggt cgcccgtatc ccggtgccgg
      301 agcgtgacgg ttccaagaag ctccggcgtc tccccgagcg tatcgaaatc aacggtcacg
      361 cagaacggcg taccgatctc gtcctgacgg cgatagcgct taccgatatt accgttgtcg
      421 tcccacgcga ttgccggatg cgccttcttg agtgcggcac gcacctcctt cgctctcgcg
      481 accagttccg gcttgttctt gagaagcggc gacaccatcg ccttcacggg cgcaaccttc
      541 ggggaaagcc ggagcaccac gcgcttctct cccccaagct cgtcctcgtc gtacgcggac
      601 gcgagtaccg cgagcatcgt gcgctcaagg ccaagcgatg gctcgatcac gtgcggcacg
      661 atcttctcct tcgcctcctc atcaagatac gtgagcgaga cgcctgaata ctcagcatga
      721 cgagagaggt cgtagtcggt gcggtacgcg aggccccaca gttccttcgt accggtcggg
      781 aactcgtact cgaagtcgat agtgcgctcg ctgtagtgtg cgcgatcctc gtcgccgatc
      841 tctttctcat gcagcttgtc cataggaagc ccgaggtcgt tggcgaatgc ctgcatctca
      901 agccgccatg cttcaaacga ctccttccat gcatctgggt gcacgaaata ctcaacctcc
      961 agctgctcga gctcacgcag gcgaaacaca aactcgcgcg gcgcgatctc gttacggaac
     1021 gccttcccta tctgccccat accgaatgga agcttcggat ggtacgcgtc gatgatgttc
     1081 ttaaagttca cgaacatgcc ctgtgccgtc tcgccgcgca ggtatgcgag cgtcgcggac
     1141 ccttgggacg ctcccatggt taccgggaac aagaggttga actttgtcgg ctgctcgtac
     1201 gcgtttccag acggggtcgt aaggcccgct gtctgaagcg cgctccacat atcttcaagc
     1261 gtcatgcctg caggatcgat gccagccgcc tcgagcacat ggtcgagacg gaagcgctct
     1321 ccagtcttcg cgtcagacac gagaggatcg ttaaacgtct ccgtgtgtcc ggacgcttcc
     1381 catacacgcg ccggcatgat gagggacgcg tccacgccgt acatgtcgtc acgatcctca
     1441 acaaaccgct tccaccagag cgccttgata ttgttcttga gcgcgacgcc caaggggccg
     1501 taatcaaagg tacctgcaag accgccgtag atctcggagc ctgggaatat gaacccgcgc
     1561 ctcttggcga gcgatacgat ctgatcaagc gatacggcca tagtacgaaa atattagcgc
     1621 acggttcctg tcccctgccc ggaggtgtcg gtggtgatcg ctgcgcctgc gccttggatc
     1681 tggccctgcg tatagcggtc gacaccctta acctcaagcg aggacatgag tacaggactt
     1741 gtaccccgct gtgaagcgct tggcaacacg gcaacctcga aggttccctg acgcgcacct
     1801 gcagagaacc cggctccgga ggcaacttcg cctgccttcc aagtgaccgt gcggctcgtc
     1861 tcgttgtacg tgatcgaagg atccgtgctg cttacgaagc gcacgtaaga cggcagtgtt
     1921 ccggtaacgg tagcgtctgc gaccgtgttc acggtgttcg atagcgcgag cacaatcgcg
     1981 tagcggcttt cctggtccgc gatcggcgga agcgggcctg cgcggtggct tacgcggcca
     2041 gagagcgcaa ggctcgtccc gaccttaagc gtgcgggtta gggacgcatt gatggtctcc
     2101 gggacgttcc cttcgcccac gcgcctgcct gcgaccgata ccgtgatatc taccgaaggg
     2161 ctgcgtgtac ctacgagtgc ctcggcgctc ttggtcttga aactgaacga tccgctgccg
     2221 gtagcgcccg gatcgagacg cgcgagaccc atctcggttt ctcgcgagta gatgatggtg
     2281 cgatcggaag aacgatagaa gcctccgtat cccgcaatgg aattcgtatc aagcgcattc
     2341 ccctcaaagc gcagcgttac ctgcgcgtca aggatgcccg cggcagtcgt atttatccac
     2401 gagatgattc cctgcaccgg tgcacccgct tcaagtaccg gagttgcgct cgtgtcgcgg
     2461 ttgaacgaga ggcttgtggc gaggaacggc tttgcgagcg tcaccggcgt tatcgcggtc
     2521 gcgtacgtga ccgcaagcag gttcgcatcc ggactcgtgc gggtacctgc agagaaccgg
     2581 agcacgcgct cctcgttgtc ttctcctgag agcgtgcccg tcaccgttac ggtgcgctct
     2641 tccccaggcg cgagcgttcc caccggaatg atagccgcct gaccacgagc tggagtgaag
     2701 ccgaatgggt actgcccgta taccgcaacg ttctcaagcg gttccttcgc gttcgatcgc
     2761 accgtgaccg cgaacgttat gggctgccct acggatgctt cggataccgc ctctgcacgg
     2821 atggaaagcg gcgaggagct tatgagaatc tcgtgcttcg tttccttcac gtatgtcgcg
     2881 tttgatccct ctacgcgata ctcgaaccga accggcacca ctacccgctc gttctctccc
     2941 ccatacaaca caacgcgcac agagcgagta gccgtctctc ctgacggcac gtcgcccatg
     3001 gtgtcctcgt aatgcacgaa cgggcgtgta ttgtcttctg gagaacgcgc ggtctccggg
     3061 aactctgcac gaatcgaggt attaaggatc gtgaccgggt tgcgattctc tacggaaata
     3121 aggagtgttg ccacgtcccc gctgcctacc gcagtctgac cgtccaccgt aagtgcgatg
     3181 cgttccgttg agacggcacg gccgccgaag acgaggaagt atgccgccac tacaagcgca
     3241 ataaggaaga aaatccctgc tgctccaagg aagatcgccg gccaagaaaa cttcggcttc
     3301 tttggctctg tgggtgctgg gggcggtgtc cacccttcag gctcgtatgc cggcgcttgc
     3361 ggacgaagcg tcgggacagg agtgctttca ggcgcctggg gggcgtatag tcgctggcgt
     3421 aagcgctcga gcgaacttgt atcgtcagga ccattcggag gcatcgctcc tcagtatagc
     3481 aagtgcttag agccccgagg ccgcgattcc gcggttgatg cgcgtcacga gctcgcgacg
     3541 ttcgtcaggc gcaagaggcg cgtactcgtt atcctccggt atagcacccg catcaagacc
     3601 taacgacgca agcatgcgga gcgccgcaaa acactcggcc acgtcctgct cttcctcttc
     3661 ccttcctgag agcgcctcaa ggaagcgaag aaatatggag aacaggaagg tatcgttcgt
     3721 ctctccgtgc acgagccgaa gcaagagccc gttcacgcgc cccgctcgct cgcgtcctct
     3781 acgatcgagc tttccaaacc agcgttcttc aaggtgcgct cctgaaagcc gccacccttc
     3841 cttgccgcgc acgagcgtga cgtcgcactg gtcaagtgtc tggagcgcgt gtgcgagctt
     3901 cgctccaggc ttccggagcc cttcagcgcg tgcgcgtagc accccgaact cctccgtaag
     3961 caaggtgagc agcgttgccg attccttaag cggcgtgcgt gcgagcacga tggcctgcgt
     4021 cgcgtactta tgacgcatcg cgcgagagcg agaagcgtac cgggcccgta gacagctccg
     4081 cgacatgcgc gccgtcctcc cgcttcgtga cggtagcgcg atctttcggc gagaagcctt
     4141 ctgccttacg tgcttcagca accgcacgct gaaacgcgcg ctcatctcct tcagcaatca
     4201 gctctggggt gagctcggta tcgagcgcca tctcttcagc gttcatgaga atctccttca
     4261 cgttgagctc ctcggcaagg atctttgcga gctcagcaga aagcactcca ggaatcgaaa
     4321 ggctcgcaag cggctgacgc actttcaggt ccgctttctg acgcaagcgg agcgcttcgg
     4381 acgcaagcgt acgggtacgc ttcatttctt caagaagtat tgcgtccttc tttgctcttc
     4441 cactaagcgc atcaaatatg cccttttcaa ctgaaggcca agacgcaaga tgcacgctct
     4501 ctggatccga gtccttacgg acaccctgat atatctcctc agcaataaac ggcatgacgg
     4561 gcgcaattgt ctttgagaag gtagcaagta ccgagcggag cattgcaagc atccgctgct
     4621 tatctccctc atcttctccc ttcgcgcgat cgcgagagcg acgtacgtac cagttagaca
     4681 gatcatctat aaaatcacga tacgcacgca cgccgcgcgg tacgtcatac cgatcaaagg
     4741 aatcagtgac atccgttacg agcgcatcaa gccgtgcctg aatccagcga tcaagaggat
     4801 ggtccgtaga agggcgcgca ttcggagcaa gctcgtcctt gtagatctca aagaatccgt
     4861 agatgttccg cagcatgttt acgacacgcg catgcgcatc cttcacgtcg tcgtccctaa
     4921 acgtgagatc ttccgcctgc atcaccacgc tcgacatgag cgtgtagcgg aaggcatctg
     4981 cgccgtactt ctcgaagaga aggtacggat cggtgtagtt gttcttgctc ttcgagagct
     5041 tcgcgccatc tgcggcaagt acgtttccgg tagtgatcac gttcttgaac gcgagatgat
     5101 cgaaaagctc gaggctaatc gcgtgcatgt agtagaacca ggcgcgcgtc tggccgatgt
     5161 actctgagat gaaatcacct ggagtacgct tctcgaacgc ctccttgttc tcaaagggat
     5221 agtggtactc cgcgaacggc atcgaaccag actcaaccca gcaatccacc acctcaggaa
     5281 tacgggtaaa cggctcgcct gcttcgtcaa cgagcgatac ccgatcaatg tatgggagat
     5341 ggtaatcgag cgtgaagtcg cgattgtgcg ggagcgggac aaacggaact tcaatgacct
     5401 ccgccggaga gaacgagctc gattcgatca gctcctttgc ttctttgttc tctagcccat
     5461 ccacaagcgc cgtgaacgat tcaagaccga taccgtgcgt cactatgaga atggtctcgt
     5521 tcgcgtacgt acgctcaagc tcgtatagga actcaccaaa gcgacgctta gcctcaaggc
     5581 cactctcgcc gccctcggga gcctcgtcat aggcgtgcgc attcctccag gcgaggaatt
     5641 cctcaaggta ccgctcttcg aattctccga acgtgagttc gcgaaggcgc tcgtcgaaca
     5701 ccagcttgtc cttagggaga ccgagctctt cacatataat ctccgcgctc tcgcgggtac
     5761 gcagaaccgg ggacgcgaag atacgtgttg gtggattcgc tttaagctcc tgtgcacggg
     5821 cgcgtacgcg ctcccttccc ttctcggtaa gcgggttctt cgtcgggtcg ctgctcaggc
     5881 gcgaaataag cgtgttctct gtctctccat gacgcatcgc gaggtagcgg ttgccactct
     5941 tcttcgtgcg ctccttgagt tccgcaagag agccgatgac gtgcactgag ccatctgcac
     6001 ccttccagat aggaagcgga ctcgcccaaa agcggttacg cgagatagtc cagtccgggg
     6061 cattctcaac gatatgcctg aagcgtccgt gcttgagatg ctccggcacc cacgtgatct
     6121 tctcgttctc tgcgagcatg cgctctttca ccgcctggat atcgatgaac caagacgaga
     6181 ccgcgttata gatgagcgcg gtgccacaac gatagcagtg cggatatgag tgcgtgtgca
     6241 tctcgcgcgc aaagagacgc ccgcgcgact cgaggtcctc cttgatctcg ctctctgctt
     6301 tcttgatgta tttcccgcgc aggaatagag gcgcgtcatc gttatacgta gcgtttggat
     6361 taaggagcgg caccatcgga agaccctcct tcacgccgag ctgatagtcg tcctctccgt
     6421 agattacggc cgtgtgcaca atgcccgtac cgtccgtggt tgtaacgaag tcagcagaaa
     6481 gcacttccca cttcttgccg gtgtggcgag ccgccttcgg tatttcgtag agaggctcgt
     6541 aggtaacacc tacaagtgcg gaacctgaga actcccgtgt cgcattcgta agaccaagtg
     6601 cttccgctcg ctccttcgcc gcaatgagat gcttcccatc cttctcatac acgccatagg
     6661 caatatcacc tcccaccgcg agcgcgacgt tgcccggaag tgtccatggt gtcgtggtcc
     6721 acgcaagaag ataggtgttc tccgggagtc cgctatcttt tgaatccaga accctgaaag
     6781 caaccgtggc ggtttcctcg gttacgtcct tgtacgtgtt gtccatcgcg atctccgcct
     6841 tagcaagcgg cgtctcgcag tgcgggcagt acatgagtac cttacgacct tcatatagtc
     6901 ttcccttttt atggatctcc ttgagtcccc accacaccga ctcgatgaac gtgttgtcca
     6961 tcgtcttgta tgaattatcg aaatctacaa aacgaccgac gcgctcaacg tagcgtttcc
     7021 agtcatgcac gtattcaagc accatggaac gtgcagtctc attaaatttc tcgatgccta
     7081 tctcaagaat ctctttcttg ttcttcaagc cgagcttctt ctctacgagc gactcgatag
     7141 gaagaccatg cgtgtcccat ccccatcggc ggcggacgcg atacccacgc atggtcttgt
     7201 agcgcggaat gagatccttc gagaccgaag aaaggagcga tccggaatgt gggaggccag
     7261 ttgcaaatgg cgggccatcg tagaacacaa actcgccctt gggcgcgtct ttgcttagag
     7321 tcttctcgaa gacttctttt tccttccaaa acgcaagtat cgcttcctca cgcttcgcga
     7381 cttctgattt ctctgtatca gccatagatt tccttatcct agcgcttcgc atatgaaaag
     7441 gaaaacggcc cgcgcaagcg cggaccgttg aaccaggttc aggacctcag gcggacgcgg
     7501 cctggagatg ctgggcgaag tgggccacct tggggtggat gcggcgggtc tgcttgtagc
     7561 gactcagcca
//
LOCUS       DASWXU010000028         9030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_739616, whole genome shotgun sequence.
ACCESSION   DASWXU010000028 DASWXU010000000
VERSION     DASWXU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739616"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..146)
                     /locus_tag="VGE48_00915"
     tRNA            complement(76..146)
                     /locus_tag="VGE48_00915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Gly,seq:ccc)
     gene            complement(152..517)
                     /locus_tag="VGE48_00920"
     CDS             complement(152..517)
                     /locus_tag="VGE48_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY1041973.1"
                     /translation="MARSSKKHVQKVSEYALAAFLGVVVLILAWAVWGIAQKEERARK
                     AVEDQKAELAALQERKQSLEANLEELSTERGQEATLRQNHGVAKAGEEVIIVVPPKDD
                     GLEPHLPWYKRFLGFFGIW"
     gene            complement(538..2325)
                     /gene="lepA"
                     /locus_tag="VGE48_00925"
     CDS             complement(538..2325)
                     /gene="lepA"
                     /locus_tag="VGE48_00925"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY1041974.1"
                     /translation="MDIRNFSIIAHIDHGKSTLADRLLERTGTIEARKMRDQVLDKMD
                     LERERGITIKMQPARMKYRAKDGKEYILNLIDTPGHIDFSYEVSRALSAVEGVILLVD
                     STQGVQAQTLTTLEMAKNAGCVIIPVLSKIDSPAARPDDVALEIAELLSIDPDTVLSV
                     SGRTGEGVEDLLETIIERVPAPRPGEGGARALIFDFSYSTHRGITVFMRVFDGSFKKG
                     DLLRFAAADERFTALETGIFMPDEVPTDNLSAGEIGYLVTGVKQAGVVNVGDTVIAST
                     GGAQGLEGFARPRPVVWASVYPESQDDLTELRQSLERLRLSDSSLAFEEESSGMLGRG
                     FRCGFLGMLHLEIIVERLKREFDLELVVTIPTIAYTVTDKKGHTEVVYTPSKFPAYGD
                     TESVSEPWVKVVLITPSDTLSSMIQLFHDHEGNVLETETHVDGRIELTVEMPLRELMR
                     GFFDKVKNASSGYASLSYELLDERPADVVKLEIYVAEEPIPAFARVVSRRRVNEEGER
                     MVEKLHEVLPRQQFVTKIQAHAMGRILASKSLSAFRKDVTGYLYGGDITRKMKLLEKQ
                     KKGKKRRGEHAKMDIPQDVFMKMVQDSER"
     gene            complement(2355..4037)
                     /locus_tag="VGE48_00930"
     CDS             complement(2355..4037)
                     /locus_tag="VGE48_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY1041975.1"
                     /translation="MVRKILSSITAPVRGLHQAAYVLAGLTLASQALALLRDRIFAHS
                     FGASDVLDLYYAAFKVPDLVFALVASLVSAYVLIPRIAGGRREESKRLISQTASFLVI
                     AGGVLCAVLAFYARPILFALFPDAIASANADGFVLLAQILLIQPILLGLSGIATSVTQ
                     VKRRFVLFALSPVLYNLGIIAGTVLLYPLYGLPGIGIGVVAGAIAHLVIHIPVLAAER
                     MLPHLVIPSPQVLWEVMRDSVPRSLALAMGSVTALILTILAARTGEGSIAVLSLALNL
                     QAVPLSLIAASYATAAFPVLSETMESKRYDAFKATLSAAARHIVFWSAILSVLILVMR
                     AHIVRIVLGTGAFDWDATRLTAAILALLIVGLMAQGLVLLASRAFYAAKKSWNPLLIQ
                     VAGLVVSVAGALGLLALATTFPGIRYFIEALLRVEDVPGTDVLLIAMGAMLGQVATGV
                     LALVTLQNVAPGVSKSLARPMLEGAAAAILGGASAYGVLSFLGNLAPATTLAYVFTEA
                     LLAGMVGLAVSAAVLALLANKEFKDLMDSLKRLRLSTALKPSGQVLSDRTDA"
     gene            complement(4056..6476)
                     /locus_tag="VGE48_00935"
     CDS             complement(4056..6476)
                     /locus_tag="VGE48_00935"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1041976.1"
                     /translation="MQEIVTRSQGGKRRLSFDMATTWAVVLTFALGAFALIPSATIPF
                     LYTKVTVLALGALVTLVLFILARLSRGNVIVPPLPLLGALWLVPAAYLLSSLFSGAGI
                     ADSFFGTELETDTLGFVLMLSAFATLTALSFRRTNSLRLFFGAGAVITGVVLAAQVLI
                     VLVGRVAPSMISPTVNLVGSFVDLGMFLGLAVVTGLLALRLLSLSSRTRTMLLVGGAV
                     SLVVLALVNSVAVWVLIALVSLALFIEAIMKNRGTGEESDLDGVALLLAEEPEETSTG
                     NRFLGIPLATLAVSLLFLVGGSTIGSSFATALGVNVIDVRPSWQSTFDIGSHTYASSP
                     LFGSGPGTFDTQWTRHKDRSLNDTIFWNVDFTTGIGYIPTSFVTTGLAGALAWLAFAG
                     LFLFIGGRFLLARLPQDPFVRFVSVASFVGSLYVLALMVFAVPGPMVLLAGFLMMGIF
                     VSSMRYGRDARELGIIFARSPKVGFVIVFALTLLLLASILAAYVAIERYLGSVAYAEA
                     ANELAAGNVEGSLNAANRALMFGESDRVYELIAGIGIARMNQIAGDTTLSQADAQAQF
                     QGALSGSIEAALAATRLGPDEYQNWAMLGNVYQTVVPLNIEGAYENAKTAYDQAIALH
                     PTSPALPYVVAQLEIARDNNQAASEALMQAIALKRDYTQAIFLLSQLEAEQGRAREAL
                     EAAEAAAYFAPNDPVVLFQVGILRSANGNTQGAIDALSRAVSANPQYANARFFLGVMY
                     AIAGQFPQAAAELEQVAALSPENAQSVAADLAAVRAGRNPFPPSRLGALGIPQGPLSE
                     SATGTGQR"
     gene            6597..6915
                     /gene="rnpB"
                     /locus_tag="VGE48_00940"
     ncRNA           6597..6915
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGE48_00940"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6954..7412)
                     /locus_tag="VGE48_00945"
     CDS             complement(6954..7412)
                     /locus_tag="VGE48_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY1041977.1"
                     /translation="MKIRTALISSIVALAVIGVLGYFFIPRAETNKQAVFERGSQNEF
                     VFEIADTDSERVQGLSGRKDISSDYGLLFVFPASGSYGFWMKDMHVSIDIAWLSEDGT
                     VLKIEENVSPSTFPAVFYPPRAVRLVLETKAGEMRKQGWDVGTRVPLPKL"
     gene            complement(7415..7705)
                     /locus_tag="VGE48_00950"
     CDS             complement(7415..7705)
                     /locus_tag="VGE48_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041978.1"
                     /translation="MADISRLSREVQWYGEEVRANERRLSDAERQQALYTRTKDEAER
                     KLTEAERMIAEGTRKIDELKRKIEADTRSLRRYESELKSAESVAREGRKRAA"
     gene            complement(7734..7820)
                     /locus_tag="VGE48_00955"
     tRNA            complement(7734..7820)
                     /locus_tag="VGE48_00955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7784..7786),aa:Ser,seq:gga)
     gene            complement(8156..>9030)
                     /locus_tag="VGE48_00960"
     CDS             complement(8156..>9030)
                     /locus_tag="VGE48_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY1041979.1"
                     /translation="KNVMKTWVLMAGFFLVVTAIGWAISWYYDSPAILWGAVIFSVLM
                     NVGSFWFSDKIALSMNGARPASREEFFDLYTITENLSITAGMPMPKLYVIEDPAPNAF
                     ATGRDEKHAVVAATTGLLQILSRTELEGVIAHELSHIRNKDMLVMTVAVVLAGFVAIV
                     ADIFLRMSLFGGGNRDNDSKAGLILAILAVVAIVLAPIAAQIIQLAISRRREFLADSS
                     GALLTRYPEGLASALQKIAGDGRPMRRANHATAHLFIGNPFGAQAGKFMNRIFATHPP
                     VEARVEALLGQKLT"
BASE COUNT         2117 a         2571 c         2539 g         1803 t
ORIGIN      
        1 gtcggggtgg tgtagatggt gacgttctta tccataggtg atgatgtgct tatgcttcta
       61 gtataccaaa ccgggtgcgg gtacggggaa tcgaaccccg atctcaacct tgggaaggtc
      121 gcattctacc actaaactat acccgctttg tctaccaaat tccgaagaac ccgaggaatc
      181 gcttgtacca cggaagatgc ggttcgagcc cgtcatcttt cggcggtacg acgatgataa
      241 cctcttctcc ggccttcgca acgccatgat tctgacgcaa cgtggcttcc tgtccccgct
      301 ccgttgaaag ctcttccagg ttcgcctcaa ggctctgctt gcgctcttgg agcgcggcaa
      361 gctcagcttt ctgatcttct acggccttcc gggcacgctc ctccttctgc gcaattcccc
      421 agaccgccca cgcaaggata agtaccacga caccaagaaa tgcagcgagt gcatactcgc
      481 ttactttctg gacatgcttt ttagaagatc gggccatgcg cccagtataa caagcgctta
      541 gcgttcgcta tcctgtacca ttttcatgaa tacatcctgt ggaatatcca tcttcgcgtg
      601 ctcgccacgg cgcttctttc cctttttctg cttctcaaga agcttcatct ttctcgtaat
      661 atcgcctccg tagaggtagc ccgtaacgtc cttgcggaag gcagacagag actttgaggc
      721 gagaatgcgg cccatggcgt gtgcttggat ctttgtaacg aactgctggc gcgggagtac
      781 ttcatggagc ttctctacca tacgctcacc ttcttcgttc acacggcggc gtgatactac
      841 acgcgcgaac gccgggatcg gttcctcagc aacatagatc tcgagcttca ccacatctgc
      901 aggacgctca tcaagaagtt catacgacaa agatgcgtac cccgaagatg cattcttcac
      961 tttatcgaag aagccgcgca tgagttcgcg aagcggcatc tcaaccgtaa gctcgatacg
     1021 gccgtccacg tgggtctccg tctcaagcac gttcccctca tgatcatgga aaagctggat
     1081 catcgaggac aaggtatcgg agggcgtaat gagtacgacc ttcacccacg gctcggatac
     1141 cgattcagtg tcaccatagg cagggaactt ggatggggta tacaccacct ccgtgtgccc
     1201 tttcttatcg gttacggtgt acgcaatggt agggatcgta accacgagct cgagatcgaa
     1261 ctctcgcttc aggcgctcga cgataatttc aaggtgaagc atgccgagga aaccgcagcg
     1321 gaacccacgc ccgagcatac ctgaggactc ctcctcaaac gccagtgagg aatccgaaag
     1381 acgaagacgt tccagagact ggcgaagctc cgtgagatca tcctgactct ccggatacac
     1441 cgacgcccac accaccggac gaggacgcgc aaagccctca agaccctgcg ctcctcccgt
     1501 cgaagcaatc actgtatcac ccacgttcac gacgcccgcc tgctttacgc cggtcacgag
     1561 gtagccgatc tctcctgcag agagattgtc cgttggtacc tcgtctggca tgaagatgcc
     1621 cgtctcaagt gccgtgaagc gctcgtcagc agcagcaaag cgcagtagat cccccttctt
     1681 aaaggagccg tcaaagacgc gcatgaacac cgtaatgccg cgatgtgtcg agtatgagaa
     1741 atcgaagata agcgcgcgcg cacctccctc gcccggacgc ggcgcaggaa cgcgctcgat
     1801 gatcgtctca agaagatctt caacaccttc gccggtgcga cctgataccg agagcaccgt
     1861 atctgggtct atagaaagga gctccgcaat ctcaagcgca acgtcatccg ggcgcgcagc
     1921 tggggagtcg atcttcgaga gcaccgggat aatcacacag cccgcattct tcgccatctc
     1981 aagcgtcgta agtgtctgtg cctgtacgcc ctgcgtcgag tctacgagaa gaatgacgcc
     2041 ttcaaccgca gaaagcgcac gtgacacttc gtacgagaag tcgatatggc ccggggtgtc
     2101 gatgaggttc aagatgtatt ccttcccgtc cttagcccga tacttcatgc gcgctggctg
     2161 catcttgatc gtgataccgc gctcgcgctc aagatccatc ttgtcgagca cctggtcgcg
     2221 catctttcga gcctcaatgg tcccagtacg ctcaaggagc cgatccgcga gggtcgactt
     2281 tccatgatcg atatgggcaa tgatgctgaa gttacgtatg tccatagaga tgcgggaacc
     2341 cctcccgttc ctctctaagc gtccgtacgg tccgaaagga cctggccgga cggcttgagg
     2401 gcggtggaga gcctgagccg cttgagggag tccatgaggt ccttgaattc cttgttcgca
     2461 aggagcgcga ggaccgccgc ggataccgct agacctacca taccagccag cagggcttcg
     2521 gtgaatacgt aggcgagagt cgtggctgga gcgagatttc caaggaacga gagcacgcca
     2581 taggcgctcg cgcccccgag gatagctgca gcggcaccct cgagcatagg gcgcgcgagc
     2641 gacttcgata cgccaggagc cacattctga agcgtcacga gcgcaagcac gccagtggcc
     2701 acctgtccga gcatcgcgcc catggcaatg agaagcacat ccgtaccagg cacgtcttcc
     2761 acacgaagca gcgcctcgat gaagtaacgg atacccggaa acgtcgttgc aagtgccaga
     2821 aggccaagag cgcctgcgac cgataccacg agtcctgcaa cctgaatcaa aagtggattc
     2881 caagatttct ttgctgcgta gaacgcgcgt gacgcgagca gtacgaggcc ctgtgccatg
     2941 agccctacga tgaggagcgc gaggatggcg gcagtgagtc tcgtcgcgtc ccaatcaaag
     3001 gcaccggtac caagcacgat acggacgata tgtgcacgca tgacgaggat gagcactgag
     3061 aggatcgcgg accagaacac gatatgacgt gcggcggcag atagtgttgc cttgaacgcg
     3121 tcgtagcgct tggattccat ggtctcagac aagaccggga atgcagcggt tgcataggac
     3181 gcggcaatca gtgagagcgg gaccgcttga aggttaagcg caagcgagag caccgcgata
     3241 cttccctcgc cggtgcgtgc tgcaaggatg gttaggataa gcgcagttac cgaacccata
     3301 gcaagcgcaa gggatcgcgg caccgaatcc cgcataacct cccacaacac ctgcggagac
     3361 ggtattacga ggtgcggcag catgcgctca gcagcaagca ccgggatatg aatcacgagg
     3421 tgcgcaattg ctccggcaac aacgcctata ccaatacctg gaagcccgta caagggatac
     3481 aggagcacgg tacccgcgat gatgccgagg ttatatagaa ctggcgaaag cgcgaacaat
     3541 acgaagcggc gcttcacctg cgttacgctc gtcgctattc ctgagagtcc gagcagtatc
     3601 ggctgaatga gaaggatttg tgcgagcaat acgaatccgt cagcattcgc agaggctatt
     3661 gcatccggga agagcgcaaa cagaatggga cgagcgtaga acgcaagcac tgcacagagt
     3721 acgcctcctg cgataactag gaacgatgcg gtctgtgata tgagacgctt cgattcctca
     3781 cgccttcctc ctgcgatgcg cggaataagc acgtacgccg acaccaagga tgccacgagc
     3841 gcaaacacta ggtccggaac tttgaatgct gcgtagtaca ggtcgagcac gtcgcttgct
     3901 ccgaagctat gcgcgaagat gcggtcacga agaagcgcaa gcgcctgaga cgcgagcgta
     3961 agtcctgcaa gcacgtacgc ggcctggtgg aggccgcgta cgggtgcggt tatggagctt
     4021 agtatcttgc ggaccatggt ctcgctagag gataactagc gctgtcctgt accggttgcg
     4081 ctttcgctaa gcggtccctg tgggatgccg agtgctccga ggcgggatgg tgggaacgga
     4141 ttgcgtcctg cacgcaccgc ggcgagatcc gctgctacag actgtgcgtt ctctggagag
     4201 agtgccgcta cctgctcaag ctctgctgct gcctgcggga actgacctgc aatcgcatac
     4261 atgacgccaa ggaagaagcg agcgttcgcg tactgcgggt tcgctgacac agcgcgtgag
     4321 agcgcatcaa tagcaccctg ggtgtttcca ttcgcggagc ggaggatacc tacctggaag
     4381 agcactaccg gatcgttcgg tgcgaagtat gctgccgcct ctgctgcttc aagagcttcg
     4441 cgcgcacgac cctgctctgc ttctagctgc gagaggagga agatagcctg ggtgtaatcc
     4501 cgcttgagtg cgattgcctg cataagagcc tcactcgctg cctgattatt gtcacgagca
     4561 atctcaagct gcgctacgac atatggaagc gctgggctcg ttggatgaag tgcgattgcc
     4621 tgatcgtacg ctgtcttcgc gttctcgtac gcaccctcga tgttgagcgg cacaacagtc
     4681 tggtagacgt ttccgagcat tgcccagttc tggtactcgt ccggtccaag acgggtcgct
     4741 gcaagcgccg cctcaataga tcctgagagc gcgccctgga actgtgcctg cgcatctgcc
     4801 tgagagagcg tggtgtctcc tgcaatctgg ttcatgcgcg cgatgccgat acctgcaatg
     4861 agttcgtaca cacgatcact ctcgccgaac ataagtgcac ggttggcggc gttgagcgat
     4921 ccttcaacat tccctgcagc aagctcgttg gctgcctcag cgtacgcaac acttccgaga
     4981 tagcgctcga tagcgacata tgctgcgagg atcgacgcaa ggaggaggag cgtgagcgca
     5041 aatacgatca cgaatcctac cttcggactg cgtgcgaaga tgatgccgag ctcacgcgca
     5101 tcgcgaccgt agcgcatcga cgacacaaag atacccatca tgaggaatcc ggcgaggagc
     5161 accatgggtc ctggaactgc gaacaccata agtgcgagta cgtagagaga accgacgaac
     5221 gacgctacag atacgaagcg tacgaacgga tcctgtggca gacgtgcaag caggaaccga
     5281 ccaccgatga agaggaagag tcctgcaaat gcgagccacg cgagcgcacc ggcaagaccc
     5341 gtggttacaa acgaggtcgg aatgtagccg ataccagtcg taaagtcgac attccagaag
     5401 atggtgtcat tgaggctgcg atccttgtga cgcgtccact gcgtgtcgaa ggttccaggg
     5461 ccagaaccga agagcggaga ggatgcgtag gtgtgactac caatatcgaa ggtagactgc
     5521 catgacggac gaacatcaat tacgtttaca ccaagcgcgg ttgcaaacga gcttccgatc
     5581 gtgctgccgc ccacaaggaa gaggagcgaa acagcgaggg ttgcgagcgg aatgccaagg
     5641 aagcggtttc cggtagaagt ctcctctggc tcttcagcaa gaagaagcgc aacgccatca
     5701 agatcgcttt cctcgcccgt gccgcgattc ttcatgatgg cctcaatgaa gagcgcaagc
     5761 gatacgagcg caatcagtac ccagacggca actgaattca cgagggcgag caccacaagc
     5821 gagacggcgc cgcctacgag gagcatcgtg cgggtgcgag aggacagcga gaggagacga
     5881 agcgcaagga gacccgttac gaccgcaaga ccgaggaaca tgccaagatc tacgaacgaa
     5941 cctacgagat taaccgtagg cgagatcatg gacggtgcaa cacgaccaac gagaacgatg
     6001 agaacctgag ccgcaagcac cacacccgtt atcaccgcgc cagccccgaa gaacagacga
     6061 aggctgttgg tgcgacggaa ggagagcgcc gtaagggtcg caaatgctga aagcatgagt
     6121 acgaagccga gcgtatcagt ctcaagttcc gtaccgaaga acgaatcagc aatccctgcg
     6181 cccgagaaca aggacgagag aaggtatgcc gcgggcacga gccacagagc accgagaagc
     6241 gggagtggcg gaacgattac gtttccacgc gagagacgcg cgagaataaa gagcacgaga
     6301 gtcacgagcg cgccaagcgc gagcaccgtc accttggtgt acagaaacgg tatcgtggct
     6361 gacggaataa gggcgaacgc gccgagcgcg aacgtaagca ccacggccca tgtagtggcc
     6421 atgtcgaagg aaaggcgtcg cttgcctccc tgcgaacggg taacaatctc ctgcatagta
     6481 ggtaattcca aagctgccct ggtaataggt tccagtatag cccgctcgcc cgctcatttt
     6541 tggaacatgt gtggaagaaa cattgatttt cttgccatat atcgtattgt tatatcggac
     6601 cctggcctga cgaccgctgc cgtaaggtag aggaaagtcc gaacacaggc tcacctcggt
     6661 gagcagtctg gtagcaggta acgcctgtcc gccgtaaggc gcgaggtgcg aacagaaacg
     6721 acggatgccg aaaggctacg tactctcttc ggagagtgtc ctagaggtga ctctaggctg
     6781 aaacggctaa atccttaccg ctgtgcaagg ccgtgcccac cctcgtgtgg gagcgccgct
     6841 tgaggtgtgt ggtgacacac atcccagata gatggtcgtc cacgacagaa ttcggcttat
     6901 gaccaggatc caacctaaat gaaagaaccc gccacaagcg ggttctttca ttatcaaagt
     6961 ttcggaagcg gcacgcgcgt tccgacgtcc catccttgct tgcgcatctc gcctgctttc
     7021 gtctcaagta caagtcgcac cgcacgcggc ggatagaata ccgctggaaa tgtactagga
     7081 gaaacgttct cctctatctt aagtaccgtc ccgtcttctg agagccatgc aatatcaatc
     7141 gatacgtgca tgtctttcat ccagaagccg tatgagccgg acgcagggaa gacgaacaga
     7201 agtccgtagt ccgaagatat atccttgcgg cctgacaaac cctgaacacg ctctgaatcc
     7261 gtatctgcga tctcaaacac aaactcgttc tggctacctc tttcgaatac tgcctgcttg
     7321 ttcgtctctg cgcgtggaat gaaaaaatat cccagcactc ctatgactgc gagcgcaacg
     7381 atacttgaga taagtgcagt tcgtatcttc atagttatgc tgcgcgcttt ctcccttcgc
     7441 gagcaacgct ttcagcagat ttaagttcac tctcatatcg acgaagcgag cgcgtatctg
     7501 cttcgatctt ccgcttcagt tcatcaattt ttcgggtgcc ttcagcgatc atgcgctcag
     7561 cttccgtgag cttgcgctcg gcctcgtctt tagtgcgagt gtataaggcc tgctgtcgtt
     7621 cagcgtctga gagcctgcgt tcattggctc gcacttcctc gccataccac tgcacttcgc
     7681 ggctcagccg gcttatatcc gccataccta atgagtgtag cactcagttg tagcggaggc
     7741 ggagagattc gaactctcga gagcctttcg accctgccac ctttccaaga tggtgcacta
     7801 gaccgctatg cgacgcctcc gtgcgcttcc gttataccac aaaagcttta gggcggcgca
     7861 tcttgcgccg ccccgttcca caacccctac ttgaccctga caccgtaggc gccgcacccg
     7921 cccttatgct tctgcaagcg aggggtgccg acgaattttg atccgctacc atggatctgg
     7981 cgtccgcctt gcgggccgcc gaattgctgg ttacttcggt atcgcctgta agcgcgcgag
     8041 agttcttccc ccgtcggtcc atcgaaagaa tcgatgggat aatcgacacc gttaacgcga
     8101 attgtatcca aaagagcctc ccgttagaac ggaagaacta tacgctaata agaaattaag
     8161 tcaatttctg tccgaggagt gcctcgacgc gtgcctcaac cggcgggtgc gtcgcgaaga
     8221 tgcggttcat gaactttcct gcctgcgcgc cgaacgggtt gccgatgaag agatgtgcgg
     8281 ttgcatgatt tgctcggcgc atcggacgtc cgtcccctgc gatcttctgg agtgctgacg
     8341 cgagtccctc cggatagcgc gtgaggagtg cacccgaaga gtctgcgagg aattcgcggc
     8401 gtcgagagat agcaagctgg atgatctgtg ctgcaatcgg tgcaagcacg atcgctacta
     8461 ccgcaagaat cgcgagaatg agtcctgctt tgctgtcgtt atctcgatta ccgccgccaa
     8521 acagcgacat gcgcaggaag atatctgcga cgatcgcgac gaatcctgcg agcaccactg
     8581 ccaccgtcat gacgagcatg tccttgttgc ggatatgcga gagctcgtgc gctataacgc
     8641 cctcaagctc cgtgcggctg aggatctgaa ggagtcccgt ggtagccgca accaccgcgt
     8701 gcttctcgtc gcgtccagta gcaaacgcgt ttggtgctgg atcctcgatc acgtagagct
     8761 tcggcatcgg catgcctgcc gttatcgaga ggttctccgt gatcgtgtag agatcgaaga
     8821 attcttcgcg tgaagcaggt cgcgcaccgt tcatggagag cgcgatcttg tccgagaacc
     8881 agaagctccc cacgttcatg aggaccgaga agataaccgc accccacagg atggcagggg
     8941 agtcgtagta ccaggagatg gcccagccta tcgctgtaac cacgaggaag aacccggcca
     9001 tgaggaccca ggtcttcatc acgttcttcg
//
LOCUS       DASWXU010000029         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_940396, whole genome shotgun sequence.
ACCESSION   DASWXU010000029 DASWXU010000000
VERSION     DASWXU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940396"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VGE48_00965"
     CDS             <1..1045
                     /locus_tag="VGE48_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY1041980.1"
                     /translation="KKVRHHFYDALLPHERNAYKPLLFTLGGVATVIALALLLQAAYF
                     VQTKVVFKETNFLASVLPGVLATLTNQDRAKAGIPALEEDAHLARAAQLKAEDMAAKG
                     YFSHVDPEGRQPWYWLDHVGYPYSYAGENLAVNFTDSEDVQEAWMNSPTHHANIVKSQ
                     YTRIGIGVAQGTYQGRETTFVVQFFSTPRASATTAAPVETPVEPASAVAAGESGGEEQ
                     VLGVEVGTVVSEPAVEPRDEASPAPEEPVAITPEESAPVSVDAATGFFAQVAASPTHT
                     LTYILAALAVLVGVLLTIAVVFHAKVAYREVLSGGFAILVVSLSLLAYNAMGTSSVKV
                     PSGGQEAAAVEAL"
     gene            1050..1745
                     /locus_tag="VGE48_00970"
     CDS             1050..1745
                     /locus_tag="VGE48_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY1041981.1"
                     /translation="MQAVILAAGRGTRMKELTDSVPKPMLTVHGKSLIEHKLEALPPE
                     VDEVIIIVGYHGDVIQNAFGNSFGGRTIRYAVQENLDGTMGALALAKPYLTDRFVVMM
                     GDDLYAREDVETCLNSPDWSVLVEETEHMGSGGRMVMDDTGHIVAIEEGDHRGKPGLM
                     NTNMLALDPRVFDYPMVPKAEGSDEYGLPQTVLAASKESGIPLWAVPATSWIQITAPE
                     DLRKAEEILSSSR"
     gene            1780..1856
                     /locus_tag="VGE48_00975"
     tRNA            1780..1856
                     /locus_tag="VGE48_00975"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1814..1816,aa:Met,seq:cat)
     gene            1907..1980
                     /locus_tag="VGE48_00980"
     tRNA            1907..1980
                     /locus_tag="VGE48_00980"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1941..1943,aa:Asp,seq:gtc)
     gene            2047..2979
                     /locus_tag="VGE48_00985"
     CDS             2047..2979
                     /locus_tag="VGE48_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1041982.1"
                     /translation="MKEYQLPEEQGHLVSYSEYGNPEGPAILSFHGGPGSGSKPQHAE
                     RFDLTKYRVILFDQRGCGKSTPLGEIENNTTDNLLRDAERIREALGIDTWFVGGSSWG
                     STLALLYALKHPERVRGLLLAAIFLADKDTISWSMNDPKGVAKLMPDVWARRMEFFTK
                     FNVRLENQNEDLLALLEGSDPETQKEVAAGIHNWEHNLFSVESQVSYKAPEEMTEGSI
                     ASAKIFVHYEKHHEFIPENHILDSIETIANIPAVIVHGRYDIVCPIDKAYALQQRMRR
                     SDFVIIPSSGHIPSEEGKVIVKLAFDRFLARLQA"
BASE COUNT          676 a          813 c          883 g          625 t
ORIGIN      
        1 taagaaggtc cgccaccatt tctatgatgc gctgctgccg catgagcgca acgcgtataa
       61 gccgcttctc ttcacgctcg gcggcgtagc aactgtcatc gcgctcgcgc ttctccttca
      121 ggcggcgtac ttcgtgcaga cgaaagttgt cttcaaggag acaaacttcc tggcatcggt
      181 ccttccggga gtgctcgcaa ctcttacgaa ccaggaccgc gcgaaggcag gcatccctgc
      241 gctcgaagag gacgcgcatc ttgcgcgtgc tgcgcagctc aaagcagagg acatggcagc
      301 gaaaggatac ttttcgcacg tggatccaga aggacgacaa ccctggtact ggctcgacca
      361 tgtagggtac ccgtacagct atgcggggga gaacctcgcg gttaacttca cggactctga
      421 agacgtgcag gaggcgtgga tgaactcgcc gacgcatcat gcgaatatcg tgaaatctca
      481 atacacgcgt atcggcatcg gcgttgcgca gggaacctac caggggcgcg agaccacctt
      541 cgtggtgcag ttcttctcaa caccacgcgc aagcgctaca acagcggcgc ctgttgagac
      601 acctgttgag cctgcgagcg cagttgcggc aggagagtca ggtggggaag agcaggtcct
      661 cggcgttgag gtgggcacgg tcgtttcaga acctgcggta gaaccaagag acgaggcgag
      721 cccggctccg gaagagcctg tcgctataac accagaagag agtgctcctg taagtgttga
      781 tgcagcgacc ggcttcttcg cccaggtggc ggcgtctccg acgcatacgc tcacctatat
      841 actcgcggcg cttgcagtgc tcgtaggtgt tttgctcacc attgcagtcg tattccatgc
      901 gaaggtcgca taccgcgaag tattaagtgg cgggttcgcg atcctggtcg tatcgctctc
      961 acttcttgct tataacgcca tgggcacctc ctcggtaaag gtgccaagtg gcggccagga
     1021 agccgctgcg gttgaggcgc tctaatcata tgcaagctgt tattcttgcc gcaggccgcg
     1081 gcacccgcat gaaagagctt acggattccg taccgaagcc catgctcacg gttcatggaa
     1141 agtcgctcat cgagcacaag ctcgaagcgc ttcctcccga agtcgacgag gttatcatca
     1201 ttgtcggcta ccacggtgac gttatccaaa acgcattcgg aaattcgttc ggaggaagaa
     1261 ccatccggta tgcggtgcag gagaacctgg acgggaccat gggcgcactc gcactcgcaa
     1321 agccgtatct tacggaccgg tttgtagtga tgatggggga tgacctgtac gcgcgcgagg
     1381 atgtcgagac ctgcctcaac tctccggact ggagtgtgct tgttgaagag acagagcata
     1441 tgggctcggg cggccgtatg gtgatggacg ataccgggca tatcgtcgct atcgaggaag
     1501 gggaccatcg gggcaagcca ggcctcatga acacgaatat gctcgcgctt gatccacggg
     1561 tctttgatta tcctatggtc ccgaaagcag aagggagcga tgagtacggc ctcccgcaaa
     1621 ccgtgctcgc tgcctcaaag gaaagcggga ttccgctgtg ggctgtcccg gctacgtcct
     1681 ggatccagat aactgcccca gaagacctca ggaaagccga ggaaatcctc tcaagcagcc
     1741 gttgaacccc tgcgctccat atagtatggt gcgtgtattg tcggggtagc tcagttggtt
     1801 agagcgtagc actcataacg ctaaggtcgt gggatcattc cccacccccg acaccagggt
     1861 tgtgtgtggc tttgaaacag gctatatttc gactcgtgcg ctatttgcgg tcgtagctca
     1921 actggttaga gcacccgcct gtcacgcggg aggttgcgag ttcaagtctc gtcgaccgcg
     1981 cacgtcacaa aaccgtccga aagggcggtt ttgtgctttg ggaacgtctc tctatactgg
     2041 attcatatga aggaatacca gcttccagaa gaacaggggc atctcgtgag ctacagcgag
     2101 tacgggaatc ctgaggggcc cgcaattctc agtttccatg gagggccagg ctcgggaagc
     2161 aagccgcagc acgcagagcg cttcgatctt acgaagtatc gcgtgattct gttcgatcag
     2221 agaggctgcg gcaagagcac gccgctcgga gaaatcgaga ataacaccac ggacaacctt
     2281 cttcgtgacg cggaacggat tcgagaagcg ctcggtatcg atacctggtt cgtaggaggt
     2341 tcgagctggg gatccacgct cgccctcctg tacgcgctca agcacccaga acgcgtacgc
     2401 ggactcctac tcgcagcaat cttccttgcg gacaaggata cgatctcatg gtccatgaac
     2461 gacccgaagg gggtggcaaa gctcatgccg gatgtgtggg cgcgtcgcat ggagtttttc
     2521 acgaaattca acgtcaggct cgagaaccag aacgaggatc tccttgcgct gcttgaaggg
     2581 agcgatcctg agacgcagaa ggaggtagcg gcagggatac ataactggga gcacaaccta
     2641 ttctcagtcg agtcgcaggt ttcctataag gcacccgaag agatgaccga aggcagcatc
     2701 gcttcggcga agatcttcgt gcactacgag aagcaccacg agttcattcc tgagaatcac
     2761 atccttgatt caattgagac tattgcgaat atcccagccg tgatcgtgca cgggcggtac
     2821 gatatcgtct gcccgattga taaggcttat gcgctacagc agcgcatgcg tagaagcgat
     2881 ttcgtaataa tcccttcaag cggccatatt ccgtctgaag aagggaaggt gatcgtgaag
     2941 ctcgcgttcg atcggttcct tgcacgtttg caggcatagc gtcaccttgc taggatt
//
LOCUS       DASWXU010000030        20307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_790718, whole genome shotgun sequence.
ACCESSION   DASWXU010000030 DASWXU010000000
VERSION     DASWXU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20307
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790718"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGE48_00990"
     CDS             complement(<1..369)
                     /locus_tag="VGE48_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1041983.1"
                     /translation="MPTAPYTLYLVDDDRFLLDMYAVKFKAAGHTVTAFSGGDELLKV
                     LREGEGPDALLLDIVMPGMTGLEILEVIKRENLKRNTKVIILSNQGQESDIEQAKTLG
                     ADGYIIKASAIPSEVFAETIK"
     gene            complement(381..2129)
                     /locus_tag="VGE48_00995"
     CDS             complement(381..2129)
                     /locus_tag="VGE48_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY1041984.1"
                     /translation="MNLLSLLTEQGLLSKEDAARVAEEARAPGNTLASALDKRGVSVE
                     KAYQAASEHYAIPYLALPQEPAAKETLGYIPEESARHYRFVPLSVADGALSVGIVDPD
                     NIEALDALQFISSRVGMPYKLFLISEADFDRVLKEYENLSGEVGEALSEFEGTTAAPA
                     DGEEKNAAPEAVETTIKADAPVTKIVATILRYAVEGGASDIHIEPTPEKTRVRFRMDG
                     ELHTSLELPAKVHAAVVARIKILSQIRLDEKRKPQDGRFSATVEGRRIDFRVSTFPTQ
                     GGEKVVMRILDSARSVATLETIGLSKADLAQIREIIRQPYGIVLIAGPTGSGKSTTLF
                     SMLSELDREASNVVSIEDPVEYEIPGVAQSQVKPEIGYTFASGLRSILRQDPDIIMVG
                     EIRDKETAQLAIQAALTGHLVFSTIHTNTAAGVIPRLVDMGVDPYLIAPTLIAAIGQR
                     LVRRLCPGAGQPVPLSESMRGLLDKEFADLPQEFHKNLPEFKQFYRASPTADCPNGTR
                     GRLAVFEFLRMDADLEHVVLTKPTEESVYAAARAKGMLTMREDAIIKALAGEIPFEEV
                     NALGGQMLIDAEETPA"
     gene            complement(2253..2561)
                     /locus_tag="VGE48_01000"
     CDS             complement(2253..2561)
                     /locus_tag="VGE48_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041985.1"
                     /translation="MALKKNTLMYIALAIVVGGAALYFLWSSQPAQPDISYMDGGPTS
                     EAQASFLTLAAQLEPIGFDASILSDPRFTALIDIKTAILPEAGGRPDPFSPLPGVAAQ
                     "
     gene            complement(2572..3144)
                     /locus_tag="VGE48_01005"
     CDS             complement(2572..3144)
                     /locus_tag="VGE48_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041986.1"
                     /translation="MISSRLLPLFFIVLAIGIFLAYVQPTFSGSVAVLQDEIESLDSA
                     LMAARQFKEKELALTQERNTLSADQLARLEAFLPDNVDNVQLIVDLNSLAARSGVTLS
                     DFDIEGGETTGTSTQPMMDSGAIPLDASDTTESLELSVSATGSYGAFRTFLAGVEQSL
                     RPLDIVELSVDDSNTGVYTYDITFRLYWLR"
     gene            complement(3141..3848)
                     /locus_tag="VGE48_01010"
     CDS             complement(3141..3848)
                     /locus_tag="VGE48_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041987.1"
                     /translation="MALPPTIPTSFVPKQPVAPSMRQKSGTNIFSVIALVLLGMSIIG
                     ALATFGYEFYLKSARDAKAAELATAQQSVDIDAVEDFVRLRDRLESVETILDKHVTLS
                     EFFDTLEARTLQNVRLSTLSISVADDRTAEIETEGVARSFNALAAQSAALAGERRIKR
                     AIFSNISVNTNGTVSFTLTASLDPRLITAGEVLPGIPDSSAPVENTGAPVQPQAPVQA
                     PATSTRATTTATTTNGL"
     gene            complement(3905..4993)
                     /gene="pilM"
                     /locus_tag="VGE48_01015"
     CDS             complement(3905..4993)
                     /gene="pilM"
                     /locus_tag="VGE48_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY1041988.1"
                     /translation="MGFLSKLFGGGKKGASVIGVDIGSSSLKVVQLRREGGAAVLETY
                     GELALGPYAGGEIGQATNLPAAKIAECLNDLLREANVTSKDAGISIPFSRSLLTLVEL
                     PRRDNPEEQKTVIELEARKYIPVPVSEVQLDWFIIPEAIPEGAAPPSKVQVLLVAVHN
                     DELTLLESVVTTSQLAATFYEIEIFSTIRSVVDEPVKPVLVLDIGASATKVYVVEHGV
                     VALSHNIPRGGQDVTRTIATASGIPVASAEALKKEHGFVDGEGTYDPRSIELVFSRIF
                     EEAKRALGQYQQTKGAQVSGLVLTGGGGVTKALQAYAKSFFNMEVHVANPFGKTQAPA
                     FMRPVLEEIGPEFAVAVGLALRKLEEQG"
     gene            5017..5640
                     /locus_tag="VGE48_01020"
     CDS             5017..5640
                     /locus_tag="VGE48_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041989.1"
                     /translation="MKLLRAILDLLFPPRDAEALVNEATSESLGALARVRTHEDGTVS
                     LLPYRAPLVKACITEAKFRDSENAQKLLAAVLADYLSSFTEDLRDYEAQNSILVPVPL
                     SEARRRERGYNQTERIGARALIGLTDITLDTRILTRTRDTLPQTSLGGRARRKNLRGA
                     FSANIPDSTHTYIVFDDVRTTGTTLTEAMAALRNANAAKIYGISLAH"
     gene            5668..5757
                     /locus_tag="VGE48_01025"
     tRNA            5668..5757
                     /locus_tag="VGE48_01025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5702..5704,aa:Ser,seq:gct)
     gene            5819..6493
                     /gene="nfi"
                     /locus_tag="VGE48_01030"
     CDS             5819..6493
                     /gene="nfi"
                     /locus_tag="VGE48_01030"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622009.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEY1041990.1"
                     /translation="MHAWNVKPRDAIALQKELAGEVKLVPLEREPSYIGGCDVSMNRF
                     AKDGFAGFVTLTYPELETVDHAVHKGVIPFPYIPGLLSFREIPMLLEAWKELEQKPDV
                     LLVDGIGIAHPRRLGIASHLGVLLDIPTVGVAKSVLVGAFEAPGNEPGDTAPLIDQKN
                     GEIIGMALRTKRNVKPMFVSPGHRITLKDAVTLVSSCVRKHRIPEPTRAAHNLMNEYR
                     RKDMVS"
     gene            6530..7387
                     /locus_tag="VGE48_01035"
     CDS             6530..7387
                     /locus_tag="VGE48_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041991.1"
                     /translation="MNKRLKIALVALLILFALIGLLFTGVFVAMQLGLTNVRGSISDR
                     NAFFLGKDTDTSATAIPSQPCVDASATTCAWNETPEWAVVEGGLEKDAAIIARVSAET
                     GVSARLIAASVVPEQIRFFTSEREVFKRYFEPLKILGSLSQFSLGVSGIKQETARLVE
                     QHALDPQSPFYPGPDAARLIAYNDQIGKDEELYRRLTDEKDHYYSYLYTALYLKEVMA
                     QWERAGFPIEDDPYALVTLFNIGFANSVPKADPKPGGAPIRVGGATYTFGQLGGDFYL
                     SNELRDVLP"
     gene            7431..7607
                     /locus_tag="VGE48_01040"
     CDS             7431..7607
                     /locus_tag="VGE48_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041992.1"
                     /translation="MNVPSIESHLSRKQEERTYPIAKRMHDLPLVGIVARSWVRATQS
                     EEPRVWASLPPVVA"
     gene            7655..8149
                     /locus_tag="VGE48_01045"
     CDS             7655..8149
                     /locus_tag="VGE48_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041993.1"
                     /translation="MNAKVIWIVLVLIGIIGIGWLAFGNRADAPAVTETATTTDMTRN
                     VSDPSMAEGEDGGPAFAVVTLTDSGFTPSTLTVNAGETVRFINESSRGMWVGSDDHPT
                     HTHYDGTSTREHCANGMATNGTFDQCTAVNHGDYWDYTFERAGSFGYHNHVGASQTGT
                     IVVR"
     gene            8151..8528
                     /locus_tag="VGE48_01050"
     CDS             8151..8528
                     /locus_tag="VGE48_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041994.1"
                     /translation="MTTNPLHLPAPELLLRVGLAISFLYPALHALTDPYAWIGYFPPF
                     LLSLAGEHTILMLHVWGAIEVVLALWLLFARHVFIPSTIMAIALMAVVIANPAQFPIL
                     FRDVTIALAAGALAWMHRPKHGA"
     gene            8518..8958
                     /locus_tag="VGE48_01055"
     CDS             8518..8958
                     /locus_tag="VGE48_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1041995.1"
                     /translation="MERRARTAGGVVLGDSGTIALVRTKGGGGWTFPKGHIEEGESDE
                     EAARREIEEETGLTDLELIDDLGSYERYRIGRNGSEDDRSELKEIHLYLFSARPHSNL
                     VPAHEIGEAEWVAMPRVMEKLTHPKDRAWFTTVFERVRHAIQRD"
     gene            complement(8991..10139)
                     /locus_tag="VGE48_01060"
     CDS             complement(8991..10139)
                     /locus_tag="VGE48_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY1041996.1"
                     /translation="MPNPKKRTPAQIVAAFKKIGPRIETKARIPLKTKEAFALFYTPG
                     VGAASSHLAARPEDARSMSVKKNSVAVISDGSAVLGLGNIGAYGALPVMEGKALIFRE
                     LAGIDAWPIVLDTQDPDEIIRTVRAIAPSFGGINLEDIKAPQCFEIERKLIESLNIPV
                     MHDDQHGTAIVVLAGLMNAAKVVEKPLKKLRIVISGAGAAGVAIAKLLKLSGIDDIVL
                     LDRGGAIYEGRPDLNHSKQELAVLTNPRKVSGELSVALAGADVFIGVSGPNLLTSAHV
                     SSMAERAIVFALSNPVPEIMPEEAKKGGAAVIATGRSDFPNQINNALVFPGVFRGALD
                     RGVMRITEESKIRAAKALAALVPKPTATKIIPDVLDKRVVPAIAKAIR"
     gene            complement(10159..11679)
                     /locus_tag="VGE48_01065"
     CDS             complement(10159..11679)
                     /locus_tag="VGE48_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY1041997.1"
                     /translation="MKRTVHLSLSVLLIGLLAFSMNVHAASKSYEETISADDLTSAYQ
                     GASAGGEKVRILIMPGHEPMYGGAEFMGYYEREFVVDIAERLKRELETDQNFEVFIAR
                     DASGWSDDFTSYFDRQMRKITRFIEDHKEDYEKFVKRGRIDVFEEQVAHNAAPGDVAT
                     RLYGVSKWANENDIDLVLHLHLNDETGHGPNERGAYSGAAIYVPDAIYGNAGASRAIA
                     EPVLERLKSTTAVSNFGYETKGIVEDRELIAVGAYNTSEAPSLLLEYSYIYEPRITGD
                     GARDAVFADFAFQTAQGVKDFFGSPGRPRLDTKALPYTFSSDLLATSTATSTNNTTGI
                     YALQALLRDLDLYPGSEASLGVCPISGLMNECTTLAVKSFQARNNLEQTGTLGPRTRA
                     ALNAAQGVVSNTPAPSAPTTSPASACAAFASDLSLDVSDADTNGEVTRLQTILARDKQ
                     IYPEGLITGFYGPATDRAVKRFQIKEGIVTASSTAYGLVGPATKAALVAACSTAGN"
     gene            complement(11753..12142)
                     /locus_tag="VGE48_01070"
     CDS             complement(11753..12142)
                     /locus_tag="VGE48_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1041998.1"
                     /translation="MTTNLSRISASFMLAALLLMGLPLAARAATQDIALTVKNITMIP
                     KPPTCEARASKKTMTSGSSVNIVWKSKDAVKMVGLVQGDKEWPADGRQKVTIAVRGKH
                     EFPMTFIGKNGATATCTAKVFVHAKNR"
     gene            12218..14392
                     /locus_tag="VGE48_01075"
     CDS             12218..14392
                     /locus_tag="VGE48_01075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY1041999.1"
                     /translation="MKPADLLTEHFTRLKPDQKKALERLGIKTIRDLLYHFPSRYEAS
                     GPTGTIGNVSPGSEVTLYGTIRKPEAKKAWKSKRPMAEAWLEDASGRIKLRWFSQPYI
                     AKTLQDGMVVKASGKVTGTEGKVYLANPEIDRSPVSPEDVHESLFKQDEATPLIEDTL
                     FAIYPESKGVSSLWLSHAVKRVFESGVHETLVDPIPKDMLDRYSLPTLQTALIWMHAP
                     RKLPDAHAARKRFAFEEVLAIQLTTQRRRLEASKERALPVHADRNRLNEFIASFPFPP
                     TEAQSSSIERIVSDFEKPHPMRRLLEGDVGSGKTAVAAATAYMVATSKPPGRIAGALQ
                     VAYMCPTEILAKQHFSTFVSYFKDHPIPIGLITGTECRKFPSKVRYEESAKVSRAQFA
                     KWVANGEIPIVIGTHALVYKSLSFQNLAYAIIDEQHRFGKAHRQKLARKGTEPMPHLL
                     SMTATPIPRTLALSIYGDLDLSLLDQMPLGRKPIITEVLGPEKRERAYERVREELKKG
                     RQAYVICPRINEPDPSKESAIQAKSVKAEAERLSRDIFPDAVIDILHSKMLPKDKEDV
                     MKRFEKNEVQILVATSVVEVGVNVPNATVILIEGAERFGLAQLHQLRGRVIRSSNQAY
                     CFALPESYGPTTKERLTALTTAKNGFELAEYDLALRGAGELYGAKQWGVSDIAMEALK
                     NVKMVEAARNEAQALIEKDPELKKYPLLATLAKEVDEEVHPE"
     gene            14446..15363
                     /locus_tag="VGE48_01080"
     CDS             14446..15363
                     /locus_tag="VGE48_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042000.1"
                     /translation="MRHSLNTLTLTNAAGMVKTKEEARTLLSVPDLSIVTVGSITLNP
                     RPGNLGETYYFDPESKTALNARGLPNPGLEETLRWLHAFREECNGASKELCVSIAGTS
                     PEEYGELTEALFSSVDILEVNAGCPNVWGPDGRKPIASYDPALLSCILTEVRSMIRDK
                     CRIGVKVSPVPDDLIPGLAGILAHHPEVSMVVATNTMPDQGGNRPDGSQALAFREREG
                     AETKNVGGLSGSPLKAHGLRVVHGLRERLMHTDVRIIGCGGIFEGQDLVDYLDAGAHG
                     VAIGTGFFESGPRIFSDVLAGAYEILEAR"
     gene            15585..16547
                     /locus_tag="VGE48_01085"
     CDS             15585..16547
                     /locus_tag="VGE48_01085"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY1042001.1"
                     /translation="MPNNGPVRTVEEALAYYAELLPFAEQTGRKIDLIMTVYHTELTT
                     PAMIEQIATSSVVRAVKHYPPHKGATTGSGYGIPLEDSHAMLRAMEETGVPLLGHFET
                     VYDASGRELPSHQREAHMVDTVLWRLRDKYPNLRICFEHASTQEAVEFVEADTSGRTV
                     MTVTPQHSLFTKADFEEHGTDLKCMPIVKTPEDRAAIVDFITSGDRRAIAGDDTAPHL
                     SKTKRTAFEDAANGCWLPHAIGLYATAFNRAGALDHRFERFMSLNGPEWWGLPAPDGQ
                     DVITLVRTSSRIPEPTRVPEEDDVVVPLGWKGTPDQMRVEFTVK"
     gene            complement(16592..16665)
                     /locus_tag="VGE48_01090"
     tRNA            complement(16592..16665)
                     /locus_tag="VGE48_01090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16629..16631),aa:Ile,seq:tat)
     gene            16753..16998
                     /locus_tag="VGE48_01095"
     CDS             16753..16998
                     /locus_tag="VGE48_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042002.1"
                     /translation="MSSRIFAPVWVIPAFFGQKGQGWAILAVIELLIFLLGARMAFWP
                     QGDLLGSERFIVLGIAAVIYVILAIIYYRTPTPIRTP"
     gene            complement(17055..19001)
                     /gene="ftsH"
                     /locus_tag="VGE48_01100"
     CDS             complement(17055..19001)
                     /gene="ftsH"
                     /locus_tag="VGE48_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY1042003.1"
                     /translation="MAPTKKPALKASKKGGPKLPKGKVPMPKGGGFMANVISTILILI
                     VLTGIYSLLMGESTKPEQISLSQVAADVKAGSVSAMEISGNEVALTYQDGTKKTSMKD
                     PEASLPETLSAYGVTPEQLSVVSTSIEKESGFAYWFFNLAPILLPILFLAVLFWFLTR
                     QVRGAGMQAFSFGQSKARMIDPADTSQRVMFADVAGAKEAKEELLEIVDFLKNPKKFL
                     DIGARIPKGILLMGAPGTGKTLLARAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDL
                     FLLAKRTSPAIIFVDEIDAVGRVRGTGIGGGNDEREQTLNQILVEMDGFDPTEKVIVM
                     AATNRPDVLDPALLRPGRFDRRVTIDLPDRKDRLEILGVHARKKPLGPDVNLEVIAER
                     TPGFSGAELYSLMNEGAILAARENRKEITQYDLIRSIEKVMLGPERKSHILGKKEKRV
                     TAYHEAGHALVGSVLEHADPIHKISIISRGRAAGYTLNLPFDDKKMQSRKSFLDDIAG
                     TLGGYVAEEMIFGDLTTGPSNDLAVIVALARDMVARYGMSDSLGPVAYAADRYGDAAY
                     SQEVAGKIDAEVARIIEEAKGRAREVLEQHRGALDAIAEKLVEVETIEREEFEKILIA
                     NGITPKAPEPEEEISAPAVGAGHK"
     gene            complement(19059..19784)
                     /locus_tag="VGE48_01105"
     CDS             complement(19059..19784)
                     /locus_tag="VGE48_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042004.1"
                     /translation="MNVRKTEIILFGVFLAMTAVYYYVSFGAENTDRIFITISTFFFS
                     IFTGFFISRQGARYSKLRETIATFDGKLSSVYRASLNVSPEVEVETGKIIKDHYEGIL
                     KARTWEAHFAQETNVISSIHRVLENRIGSEKVESLRNQAIGRMMTGLSDCQVLRKNMV
                     MLYQERIPSFQWFLIVFFLLMLLMSISVIPSIGMLLGSMLKSAFVVSLISVIIILRNL
                     DNLHLFEGFIGESSARDVVVNIR"
     gene            19861..20307
                     /gene="smpB"
                     /locus_tag="VGE48_01110"
     CDS             19861..20307
                     /gene="smpB"
                     /locus_tag="VGE48_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY1042005.1"
                     /translation="MAPLLENKKVRLRFESLETFTAGIELFGFEVKSLRAKLGSLEGS
                     RVVVRGGEAFLTGMTIPPYQMANAPKDYDPERTRRLLLSRKEIAELLDAESKKGLTIV
                     PFEVYNKGRYLKVRVDIVRGKNKADRREDIKRLEAKKEADRAMRAK"
BASE COUNT         4461 a         5959 c         5626 g         4261 t
ORIGIN      
        1 cttgatagtt tctgcgaaca cctcagatgg gattgccgat gctttgatga tgtacccatc
       61 tgcgccgagc gtctttgcct gctcgatatc cgattcctgt ccctgattgg aaaggatgat
      121 tacctttgtg tttcgcttaa ggttctcgcg cttgattacc tcgaggatct caaggcccgt
      181 catgcctggc atcacgatat caaggagaag tgcatcagga ccttcgcctt cgcgaagcac
      241 tttgagaagc tcatcgcctc ctgagaacgc agttaccgta tgccctgccg ccttgaactt
      301 aacggcatac atatcaagga gaaagcgatc gtcatcgacc agatagagcg tgtacggggc
      361 ggtaggcatg attcaggttc ttaggcaggc gtctcttcag catcgatgag catctgtccg
      421 ccgagggcgt tcacttcctc aaacggaatc tctcctgcta acgccttaat gatagcatcc
      481 tcgcgcatcg tcaacatgcc cttagcgcgt gctgccgcat acacactttc ctcggtcggc
      541 tttgtgagaa cgacgtgctc gagatccgca tccatgcgca agaactcgaa caccgcaaga
      601 cgtcctcgcg taccattcgg gcagtctgcg gtagggcttg cgcggtagaa ctgcttgaac
      661 tcggggagat tcttatggaa ttcctgcggg aggtccgcaa actccttatc caggagcccg
      721 cgcatactct ctgaaagcgg tactggctgc cctgccccag gacagagcct tctgacaagg
      781 cgttgaccaa tcgccgcgat aagggtcggc gcaatgagat acggatccac gcccatgtcc
      841 acgagacgcg ggataacgcc cgctgcggta ttcgtgtgaa tggttgagaa cacgaggtgt
      901 cccgtaagcg cggcttggat cgcaagctgg gccgtttcct tatcgcgaat ctctcccacc
      961 atgatgatgt cagggtcctg gcggagaatg gagcgaagac ctgacgcaaa cgtgtaacca
     1021 atctctggct tcacctgcga ctgcgcaaca cccgggatct cgtactcaac cgggtcttca
     1081 atggaaacca cgtttgaggc ttcgcggtcg agctctgaga gcatcgagaa caaagttgtc
     1141 gacttaccag aaccggtcgg tcccgcgata agcacgatgc cgtacggctg gcggataatc
     1201 tcacggatct gtgcgagatc ggctttcgag agaccaatcg tctcgagagt cgcaaccgag
     1261 cgcgcgctat caagaatacg catcactacc ttctcgcctc cctgtgttgg gaaagtagaa
     1321 acacgaaagt cgatacgacg tccttccacg gtcgcagaga agcggccgtc ctgtggcttt
     1381 cgcttctcat caagacgaat ctgcgaaagg atcttgatac gcgctactac ggctgcatgc
     1441 accttcgccg gaagctcgag cgaggtgtgg agctctccat ccatgcggaa tcgcacacgt
     1501 gtcttctctg gggtcggctc aatgtgaata tcagatgcac caccctctac ggcataccgc
     1561 aggatcgtcg caacgatctt cgtgacaggc gcgtccgcct tgatagtggt ttcaactgcc
     1621 tcaggagccg cattcttctc ctctccatca gcgggcgcag cagtcgtacc ctcgaattcc
     1681 gagagtgctt cgcccacctc cccggaaaga ttctcgtact ccttgagtac acgatcgaag
     1741 tccgcctccg agataaggaa gagcttatag ggcatgccca cacgactcga gatgaactgg
     1801 agcgcatcga gtgcttcgat attgtctggg tcaacgatac cgactgagag cgcgccatct
     1861 gctaccgaaa gcgggacgaa acggtagtgc cgcgcggact cttcaggaat ataaccgagc
     1921 gtctctttcg ctgccggttc ctgcgggagt gcgaggtatg ggattgcata gtgctcactt
     1981 gcagcctgat aggccttctc aaccgatacg ccacgcttat ccagcgcaga cgcaagcgta
     2041 ttcccaggcg cacgcgcctc ctcagcaacg cgtgccgcgt cctcctttga aagaagcccc
     2101 tgttccgtaa gcagcgaaag aagattcatc gcgtaggcct tcgcggagga ttgctcctgc
     2161 cgcaacgcat cgttatgcac cctggtatgt acgtagtgta acgcgtaaga ggcacgttgc
     2221 cgtgcctctt catacgaacc tgtgggatat cgctactgtg ccgcgacgcc agggagcggc
     2281 gagaacggat caggacgtcc gcctgcttct ggaagaatcg cggtcttaat atcgataagc
     2341 gcggtaaagc gaggatcgct caggatgctt gcatcaaagc caattggctc gagctgtgcg
     2401 gcaagtgtaa ggaaggaagc ctgtgcctcg ctcgtaggtc ccccatccat gtacgagatg
     2461 tcaggctgtg ctggctggct tgaccagagg aaatagagag cagctccgcc aacaacgatg
     2521 gcgagagcga tatacatgag ggtgttcttc ttaagcgcca taagagtacg gttatcggag
     2581 ccagtagagg cggaacgtta tgtcatacgt gtatacgccc gtattcgagt catcaaccga
     2641 aagttccacg atgtcgagcg gacgcaagct ttgctccacg cccgcaagga acgtgcgaaa
     2701 tgccccgtag cttccggtag cggataccga cagctcgagt gactctgtgg tatcagatgc
     2761 atcaagcggg atcgcaccac tgtccatcat cggctgcgta ctcgtccccg tggtctcccc
     2821 tccctcaata tcaaaatcgg acagtgtcac gcccgaacgt gccgcaagcg agttcaagtc
     2881 gacgatgagc tgcacattat caacgttgtc tggaaggaac gcttcaaggc gcgcgagctg
     2941 gtctgctgaa agcgtattgc gctcctgtgt gagtgcaagc tctttctcct taaactgccg
     3001 cgctgccatg agtgccgaat caaggctctc aatttcatcc tgaagcaccg cgacagaacc
     3061 actgaacgtt ggctgcacgt atgcgaggaa tattcctatc gccaacacta tgaagaagag
     3121 tgggagaagt cgtgaggaga tcataggccg ttggtagtag ttgcggttgt cgtggcacgg
     3181 gtgcttgttg ctggtgcctg tactggagcc tgcggctgca caggagctcc agtattctca
     3241 actggcgccg aggagtcagg gatacccggc aatacttcgc ctgcggttat gaggcgcgga
     3301 tcgagggacg ccgtaagcgt gaatgagacc gtgccattcg tgtttacaga gatattcgag
     3361 aatattgccc gcttgatacg acgctcccct gcgagggccg cactctgtgc cgcgagcgca
     3421 ttgaatgaac gagcgacgcc ttcggtctca atctccgcgg ttctatcgtc cgctactgag
     3481 atagagagcg tgctcaaacg cacgttctgg agcgtgcgag cttcaagcgt atcgaagaac
     3541 tcagaaagcg tgacgtgctt gtcgagaata gtctcgaccg attcgagccg atcacgaagg
     3601 cgcacaaagt cctctaccgc atcgatatcg acgctctgct gcgccgtagc gagctccgct
     3661 gctttcgcat cacgcgcgct cttgagataa aactcgtacc caaatgttgc gagcgcacca
     3721 atgatgctca tgccaagcag cacaagtgca atgacagaaa agatgttcgt accactcttc
     3781 tgtcgcatcg atggtgcaac aggctgtttg ggtacgaatg acgtcggtat ggtcggagga
     3841 agggccacag tagtatcagt atacgaggcg cgggcgcggt ttacgccgct ttatgcacag
     3901 cgcactatcc ttgctcctca agcttacgaa gcgcaagtcc cacagctacc gcaaactcag
     3961 gaccgatttc ttcaaggact ggtcgcatga aggccggagc ctgggtcttc ccgaacgggt
     4021 ttgccacatg cacttccata ttgaagaagc tctttgcata cgcttggagc gccttcgtga
     4081 cgccgccgcc gccagttagt acgagcccgg agacttgggc gcccttagtc tgctggtact
     4141 gccccagggc acgctttgct tcctcgaaga tacgcgagaa cacgagctca attgagcgcg
     4201 ggtcataggt tccttctcca tcaacaaacc cgtgctcttt cttgagcgct tctgcgcttg
     4261 caaccggaat gccgcttgcg gttgcgatag tacgggtcac gtcctgtccg ccgcgtggga
     4321 tgttatgcga gagcgctact acaccgtgtt caaccacata taccttggtt gcagacgcac
     4381 cgatgtcgag caccaatacc ggtttcaccg gctcgtctac tactgagcga atcgtgctga
     4441 agatctcgat ctcgtagaag gtagcggcaa gctgcgatgt tgttacaaca ctctcaagaa
     4501 gggtgagctc gtcattgtgc accgcaacga ggagcacctg caccttggaa ggaggtgcgg
     4561 cgccttctgg gatcgcttca gggatgataa accagtcgag ctgcacctcg gatactggta
     4621 ccggaatgta tttgcgcgct tcaagctcga taaccgtctt ctgctcctcg ggattgtctc
     4681 gacgcggaag ctcaacgagc gtaagcaagg aacgtgagaa cggtatggag ataccggcgt
     4741 ccttagaggt cacgtttgct tcacgtagga ggtcgttcaa gcactcagcg atcttcgccg
     4801 caggaaggtt cgttgcctgc ccaatctcgc caccagcata gggtccgagt gcgagctccc
     4861 cgtaggtctc aagtactgca gcaccgcctt cacgacgcag ctgcaccact ttcaaagacg
     4921 acgagcctat atcgacaccg atcacgctcg cgcctttctt tcctccgccg aagagtttag
     4981 agaggaatcc catcgcgcga attgtatcat gcgtacatga agcttctccg ggcaatcctc
     5041 gaccttctct tcccaccgcg cgatgccgag gcacttgtga acgaagcaac gagcgaatcg
     5101 ctcggagcac tcgcacgcgt ccgcacccat gaggacggaa ccgtgtcatt acttccctat
     5161 cgcgcaccct tggtgaaagc atgcataacc gaagcgaagt tcagggatag cgagaacgcg
     5221 caaaagctcc ttgcagcagt ccttgcagac tacctttcct cctttacgga agacttgcgg
     5281 gactatgaag cgcagaatag tatcctggtg cccgtaccgc tctcagaagc acgtcgcagg
     5341 gaacgcggct ataaccagac agagcgcata ggtgcacggg ctctcatagg actcactgac
     5401 atcacgcttg atacccggat cctgacgcgt acgcgcgata ccttgccaca aacttcgctc
     5461 ggaggccgag cacgcagaaa gaacctaagg ggtgccttta gtgcgaatat cccggattca
     5521 acccatacct atatagtgtt cgatgacgtc aggaccacgg gcaccacgct cacggaggct
     5581 atggcagcgc ttaggaatgc caatgcagca aaaatatacg gcatttcatt ggcgcattag
     5641 ggcggaaatg atactttgat acctacagga gacgtggctg agtggtcgaa agtacctcac
     5701 tgctaacgag gcgagctgta aaaggctccg agggttcgaa tccctccgtc tccgccaaat
     5761 cgaaagcccc attcggagct ttcagccgtt tctcaccttc cgtccgtaca atctctgcat
     5821 gcacgcatgg aacgttaagc ctcgagacgc gatcgcgctc cagaaggagc ttgccggaga
     5881 agtgaaactc gtaccacttg aacgtgagcc ttcgtatata ggtggctgtg acgtatccat
     5941 gaaccgtttc gccaaagacg ggttcgcggg attcgtgaca ctcacgtacc cggaacttga
     6001 aacggtagac catgccgtac acaaaggcgt cattcccttc ccctacatcc caggactcct
     6061 ctcgttccgc gagataccca tgctgcttga agcgtggaag gaactcgaac agaaacctga
     6121 cgtgctgctc gtggacggca tcggcatcgc gcacccgagg cgtctcggta tcgcctcgca
     6181 cctcggcgtg ctccttgata taccgaccgt tggtgtcgcc aagagtgttc tcgtgggagc
     6241 cttcgaagct ccgggcaacg agccaggcga taccgcaccg ctcatagatc aaaagaacgg
     6301 cgagatcatc ggcatggcgc ttcgaacgaa acggaacgtg aagccgatgt tcgtatctcc
     6361 cggacaccgc ataaccctta aggatgcggt tacgctcgtc tcttcctgcg tcaggaagca
     6421 ccgcatcccg gaaccaaccc gcgcggcgca caacctcatg aacgagtacc ggcgtaagga
     6481 tatggtctcc tgattcgtct tcgtacgcga tacctggtat acaacctaca tgaacaagcg
     6541 tctcaagata gcgctcgtag cgctccttat cctctttgcc ttgatcggcc tccttttcac
     6601 tggagtcttc gtcgccatgc agctcggact caccaacgtc cgcggctcga tctcagaccg
     6661 taacgcgttc ttcctcggca aggatacgga cacgtctgct actgcgatac catcccagcc
     6721 ttgcgttgat gcaagcgcaa ctacatgcgc atggaacgag acgcctgagt gggcagttgt
     6781 tgagggcggt ctagagaaag acgctgccat cattgcgcgc gtatccgctg agacgggtgt
     6841 ctcagcgcgc ctcatcgccg caagcgtggt cccagagcag atccgtttct tcacctccga
     6901 acgcgaggta ttcaagcgct acttcgaacc actgaagatc ctcggttcgc tctcccagtt
     6961 ctcgctcggc gtatccggca ttaagcagga aacagcgcgt ctcgtagagc agcatgcgct
     7021 tgatccacaa tctccgtttt acccgggacc ggacgccgcc cgcctcattg cgtacaacga
     7081 ccagattggc aaggatgagg agctctaccg acgccttacc gacgagaagg accactacta
     7141 ttcctacctc tacaccgcgc tctacctaaa ggaagtcatg gcgcagtggg agcgtgcagg
     7201 attcccgatc gaggacgacc cgtacgcgct cgtgacgctt ttcaatatcg gcttcgcgaa
     7261 ctccgtgcca aaagctgacc caaagccagg cggcgcgcct atcagggttg gcggcgcaac
     7321 atacaccttc ggacagctcg gcggcgactt ctacctttcg aacgaactgc gcgacgtcct
     7381 tccgtagctt caccactccg tcatcaatac gtgcgtatag tacggagcgt atgaacgttc
     7441 cgagcatcga gtcacatctc tcacgcaagc aggaggagcg tacgtatccg atcgcaaagc
     7501 gcatgcacga tctaccgctc gttggcatcg ttgcgcgctc gtgggtacgc gccacgcagt
     7561 ctgaagagcc tcgtgtatgg gcaagcctcc ctccggtcgt cgcgtagagc acggtacact
     7621 ataggaagcc aattccggct taacctattt gctcatgaat gccaaagtca tctggatcgt
     7681 gctcgtgctt atcggaatca ttggtatcgg ctggcttgcc ttcgggaacc gtgcggacgc
     7741 gccagctgtc actgaaactg cgacgactac tgatatgacc cgaaacgtga gcgatccctc
     7801 catggctgaa ggcgaggatg gcggacctgc atttgccgtc gtgacgctca ccgattcagg
     7861 attcactccg agcacgctca ccgtaaatgc tggagaaaca gtgcgcttca tcaacgaatc
     7921 gtcccgcggt atgtgggtag gttctgacga ccatccgaca catacccatt acgatggcac
     7981 ttccacccgt gagcactgcg cgaacggcat ggctaccaac ggcaccttcg accaatgcac
     8041 ggccgtgaac catggcgatt actgggacta tacgttcgag cgcgcaggct cattcggata
     8101 ccacaatcat gtgggtgctt cccagaccgg tacgatcgta gtgcgctagc atgactacga
     8161 acccgctcca ccttcctgcg cccgagcttc ttctgcgcgt aggtcttgct atctcgtttc
     8221 tgtatccagc gctccatgcg ctcacagatc cgtacgcatg gatcggctac ttcccgccat
     8281 ttctgctctc gcttgccgga gagcacacta ttctgatgct ccatgtatgg ggcgcaatcg
     8341 aagtagtgct tgcgctttgg ctgttgtttg cgcgacatgt ctttattccg agcaccatta
     8401 tggctatcgc gcttatggcc gttgtgatcg cgaatcccgc gcagttccct atcctcttcc
     8461 gtgacgtgac aattgcgctc gctgctggtg cgctcgcgtg gatgcaccgc ccaaaacatg
     8521 gagcgtagag cacgtaccgc aggaggcgtg gtgcttggag acagtggcac catagcgctc
     8581 gtacgcacca aaggcggagg cggatggacg tttccgaagg gacacatcga ggagggcgaa
     8641 tccgatgagg aagcagcgcg tcgcgagatt gaggaagaaa ccggacttac cgatctcgag
     8701 ctcattgatg acctcggttc ctacgaacgc taccgcatag gaaggaacgg ttcagaagat
     8761 gaccgttctg aactcaaaga gatacatctc tacctatttt cggcacgccc acactcaaat
     8821 cttgtccctg cacacgaaat cggcgaggcc gaatgggtag ctatgccgcg cgtgatggag
     8881 aagctcacgc atccaaaaga ccgcgcgtgg ttcacgaccg tcttcgagcg cgtacgacac
     8941 gcaatccagc gagactaacg aaacaggccc gcgtacgcgg gcctgtttcg ttagcgaatc
     9001 gcctttgcaa ttgcgggcac cacccgctta tccaacacgt cagggataat cttcgttgcc
     9061 gtaggctttg gaacaagcgc cgcaagcgcc ttggccgcac ggatcttaga ttcctctgtg
     9121 attcgcataa cacctcgatc aagtgcgcca cggaacacac ccgggaacac gagggcattg
     9181 ttaatctggt tcgggaaatc tgagcgaccg gtcgcgataa ccgctgcacc tcccttcttt
     9241 gcctcttccg gcatgatctc tgggactgga ttggagagcg cgaacacgat agcgcgctcc
     9301 gccatggacg agacatgtgc actcgtaagg agattcggcc cggagacgcc tatgaagaca
     9361 tctgccccag caagcgccac agagagttca cccgagacct tacgcggatt cgtgagtacg
     9421 gcgagctcct gcttgctgtg attgagatca gggcgtcctt cgtagatagc gccgccgcga
     9481 tcaaggagca cgatgtcatc gatgccagag agcttaagga gctttgcaat agcgacccct
     9541 gcagcgccgg cacctgagat aacgatgcga agtttcttga ggggcttttc tacaaccttc
     9601 gctgcgttca tgagtcctgc gagcacgacg atggcagtgc cgtgctggtc gtcatgcata
     9661 accgggatgt taagcgattc gataagcttc ctctcaatct caaagcactg cggtgccttg
     9721 atgtcctcga gattaatgcc gccgaacgaa ggcgcaatgg cgcgcaccgt gcggatgatc
     9781 tcgtccgggt cctgggtatc gagcacgatc ggccacgcat cgattccggc aagctcgcgg
     9841 aagataagtg ccttcccttc cataaccggg agtgcgccgt atgctccgat attcccgagc
     9901 ccgagtaccg ctgagccgtc tgagataaca gctaccgaat tcttcttaac agacatagaa
     9961 cgcgcatctt ccgggcgtgc cgcaagatgc gatgaggctg cacctacgcc gggtgtgtag
    10021 aagagcgcga acgcttcctt agtcttaagc ggtatgcgcg ccttcgtctc gatgcgcgga
    10081 ccgatcttct taaaggcggc gactatctgc gcaggagtgc gcttcttggg gtttggcatg
    10141 tcgaagagta tcccacgcct agttccccgc tgtcgaacat gccgctacaa gcgcagcctt
    10201 agtggcagga cctacgaggc cgtatgcagt cgaggaggca gtgacaatcc cttccttaat
    10261 ctggaagcgc ttcacggcac ggtccgtagc aggaccgtag aagcctgtaa taagtccctc
    10321 cgggtatatc tgcttatccc tagcaaggat ggtctggagc ctggtaacct cgccgttggt
    10381 atctgcatcc gatacgtcga gcgaaaggtc gctcgcaaag gcggcacatg ccgaggctgg
    10441 cgacgtcgtc ggagcagatg gggccggtgt gttagagaca acgccctgtg ctgcattaag
    10501 tgctgcacgg gtacgaggtc cgagcgtccc cgtctgctca aggttatttc gtgcctggaa
    10561 tgacttcacc gccagagtag tacactcgtt cataagccct gagatcggac acacaccgag
    10621 cgacgcttcg cttcctggat agagatcgag atcccgaagg agtgcctgga gcgcatagat
    10681 accagtagtg ttgttcgtac tggtcgcggt cgaggtcgca agcaggtcgc tactaaaggt
    10741 gtacggaagt gccttcgtat ctaaacgtgg acgtcccggc gaaccgaaga aatcctttac
    10801 gccctgcgca gtctggaatg cgaagtccgc aaacaccgca tcgcgcgcgc cgtccccggt
    10861 tatgcgcggc tcatagatat aactgtactc aagaaggagg ctcggcgctt cactcgtgtt
    10921 gtacgcaccc acagcgataa gttcgcgatc ttcaacaatc cccttagtct cgtaaccgaa
    10981 gtttgatact gcagtcgtcg acttcagacg ctctaacacc ggttctgcta tcgcgcgcga
    11041 cgcacccgcg tttccatata tcgcgtctgg cacgtagatc gcggcgcctg agtatgcccc
    11101 gcgctcgttt ggcccatgtc cggtctcatc gttcagatgg aggtggagca ccaggtcgat
    11161 atcgttctcg ttcgcccact ttgagacgcc gtagagacgg gtcgccacgt ctccaggagc
    11221 agcgttgtgc gctacctgtt cttcgaacac atcgatcctg ccacgcttca cgaacttctc
    11281 gtagtcctcc ttatgatcct caataaagcg agtgatctta cgcatctggc ggtcgaagta
    11341 cgaagtaaag tcgtcgctcc atccgcttgc atcgcgtgcg atgaatacct cgaagttctg
    11401 atcagtctcg agttcgcgct tcaagcgctc tgcgatatcc acgacaaact cacgctcgta
    11461 gtagcccatg aactccgcgc cgccgtacat cggctcatgc cctggcatga tgagtatgcg
    11521 caccttctcc cctccagcag aggctccctg ataggcgcta gtgagatcat ctgcactgat
    11581 cgtttcctca tatgatttag acgcggcgtg cacgttcatg ctgaacgcga gcaggccgat
    11641 taagagaacg gagagggaaa gatgtacagt gcgcttcata aaagtacggt atcacgtata
    11701 caaacaatcc ccagtctcgc tgaccgggga tgatgcgggg atgaagagtc gcttagcggt
    11761 tctttgcgtg cacgaagacc ttggcggtgc aggtcgcggt agcgccgttc ttgccgatga
    11821 aggtcattgg gaactcgtgc ttgccgcgga ccgcgatcgt gaccttctgg cgtccgtctg
    11881 ccggccactc cttgtctcct tgtacgagac cgaccatctt cacggcgtcc ttgctcttcc
    11941 agacaatatt tactgaactg ccggaggtca tggtcttctt cgatgcacgt gcctcgcagg
    12001 ttggcggctt cggaatcata gtgatgttct tcaccgtgag cgcgatgtcc tgagtggcag
    12061 cacgcgctgc gagcggaagc cccatcaaaa ggagcgctgc gagcatgaag gatgcgctga
    12121 tgcgcgatag gttcgtggtc ataggtatat atatgagagc attcttcatc gaaaaatgcc
    12181 agatgccctg actggtcctc gagcgtatac tctatctatg aagcctgcgg acctgctcac
    12241 cgaacacttc acgcgcctca agcctgatca gaagaaggcg cttgagcgcc tcggtatcaa
    12301 gactatccgc gaccttctct accacttccc ctctcgctac gaggcatcag gcccgacagg
    12361 aaccatcggc aatgtctcgc ccggaagcga agtgacccta tacggcacaa tcaggaagcc
    12421 cgaagcgaag aaagcatgga aatcgaagcg tccgatggct gaagcctggc ttgaagacgc
    12481 atcaggtcgc atcaagctgc gctggttctc gcagccgtac atcgcaaaga cccttcagga
    12541 cggcatggta gtgaaggcaa gcggcaaggt aaccggaacc gagggcaagg tgtaccttgc
    12601 gaatcctgag atagatcgtt cgccggtctc gcctgaggac gtacacgaat cgctcttcaa
    12661 acaagacgaa gcgacaccac tcatcgaaga tacgctcttt gcgatctatc ccgaatcgaa
    12721 gggcgtatct tccttatggc tttcgcacgc cgtgaagcgc gtatttgaat caggcgtaca
    12781 cgagacactt gtcgatccga taccgaaaga catgctcgat cggtacagcc tccctacact
    12841 ccagaccgca ctcatctgga tgcatgcgcc ccggaagctt cctgacgcgc acgcggctcg
    12901 caagcgcttc gcgtttgaag aggtgctcgc catccagctc accacccagc gcagaagact
    12961 tgaagcatct aaggaacgtg cgcttccggt gcatgccgat cgcaatcgct tgaacgagtt
    13021 catcgcgtcc ttccccttcc cgccaaccga agcgcagtct tcatcgatcg agcgcatcgt
    13081 gtctgacttc gagaagccgc acccgatgcg ccgcttgctc gagggcgacg taggaagcgg
    13141 caagaccgcg gtagcggcgg caaccgcgta catggtagcg acatcaaagc cgcccggaag
    13201 gatcgccggc gcgctccagg tcgcgtatat gtgcccaact gagattcttg ccaagcagca
    13261 tttctctacc ttcgtctcct atttcaagga ccatccgatc cctatcggtc tcataaccgg
    13321 taccgaatgt aggaaattcc cctcgaaggt ccggtacgag gagtcagcga aagtatcgcg
    13381 tgcgcagttt gctaagtggg tagccaatgg cgagatcccg atcgtgatcg gcacgcacgc
    13441 gctcgtctac aagtcgctct ccttccagaa cctcgcgtac gccatcatcg acgaacagca
    13501 tcgcttcgga aaggcgcacc gccagaagct cgcgcgcaaa ggtacggaac ccatgccgca
    13561 cctcctttcg atgaccgcga cgcctatccc gcgtacgctt gcgctctcaa tctacgggga
    13621 cctcgacctt tccctgctcg atcagatgcc actcggccga aagcccatca tcacggaagt
    13681 cctcggtccc gagaagcgtg agcgcgcata tgagcgcgtg cgcgaggaac tcaagaaggg
    13741 acgtcaggca tacgtcatct gcccgcgtat caatgaaccg gatccctcga aggaatccgc
    13801 catccaagcg aagagcgtga aagcagaagc ggagcgcctt tcgcgcgaca tcttccctga
    13861 cgccgtgata gacatcctgc actcgaagat gcttccaaag gacaaggagg acgtcatgaa
    13921 gcgattcgag aagaacgagg tgcagatact ggttgccaca tctgtagttg aggtgggcgt
    13981 gaacgttccg aacgcaaccg tgatccttat cgaaggcgcc gagcgcttcg ggctcgccca
    14041 gctgcatcag ctgcgcggtc gcgtgatccg ctcctcgaac caggcatact gcttcgcgct
    14101 tcctgagtcc tacggcccga ctacgaagga gcgtctgacc gcgctcacta ccgcaaagaa
    14161 cggcttcgag ctcgccgagt acgatcttgc acttcgcggc gcaggagagc tgtatggcgc
    14221 aaaacaatgg ggagtatccg acatcgccat ggaagcctta aagaacgtga agatggtgga
    14281 ggcggcgcgc aacgaggcac aggcgctcat agagaaagat ccggaactga agaagtatcc
    14341 acttcttgca acactcgcaa aggaagtgga cgaggaggtg catcctgaat aagcctttgt
    14401 tacagtgcgc tcggaagcac cctcaatcat gaaaggagga ctcacgtgcg ccattctctc
    14461 aacaccctca cgctcacgaa tgccgcgggc atggtgaaaa ccaaggaaga agcgcggact
    14521 ctcctctcgg ttcctgatct ctcaatcgtc accgtgggtt caatcaccct gaacccgagg
    14581 cccggcaacc tgggagagac ctactacttc gatccggaat cgaagaccgc actcaacgcg
    14641 cgtggtctcc cgaacccggg gctcgaggag actctgcgct ggcttcatgc gttccgcgag
    14701 gaatgtaacg gcgccagcaa ggagctctgc gtgagcatcg ccggcacctc acccgaggaa
    14761 tacggcgaac tcactgaggc cctgttctct tcggtcgaca ttctcgaggt gaatgccggg
    14821 tgtccgaatg tgtggggacc tgatggacgc aaacccatcg cgtcctatga cccggccctg
    14881 ctgtcgtgca tcctaaccga ggtcagaagc atgatccggg acaagtgtcg gatcggcgtt
    14941 aaggtctcac ccgtcccgga cgatctcatt cctggtcttg ctggaatcct ggctcatcat
    15001 cccgaggtct ctatggtggt tgccaccaac accatgccgg accagggcgg caaccgacct
    15061 gacggatcgc aggcgctcgc gttccgcgaa cgggaaggag cagagaccaa gaatgtcggc
    15121 ggtctaagcg gttctccgct caaggcccat ggcctgcgcg tcgtgcatgg gctcagagaa
    15181 cgccttatgc acacggacgt ccgtatcatc ggatgcggag gcatctttga aggccaagac
    15241 ctcgtggact atctagacgc cggagcccac ggggtcgcta tcggcaccgg attcttcgag
    15301 tccggtccga gaatcttctc cgatgtgctt gccggcgcgt atgaaatcct cgaggcgcgt
    15361 taaccggtct gtgcggcggt cccaagtgac cgccgcattc ccttttcata tccctggtgc
    15421 tacagtccgc gaagacccat tcagcccttt ttgaaaggaa ccgggcgatg caaaaccgaa
    15481 ccacatttcg acgatgctcg aatttccacg cgcatctgag gcgtgatgcc cttatgcggg
    15541 cggtcgcacc tgagatcatg cgccatatgc gatatgtgct ggcgatgccc aacaacggcc
    15601 ccgtccgcac cgtcgaggaa gcgctcgcgt actacgcgga gcttctgcca ttcgccgagc
    15661 aaaccggccg gaagatcgac ctgatcatga cggtctacca cacggaactc acgacgccgg
    15721 ccatgatcga acagatcgcg acctcaagcg tcgtgcgcgc cgtgaagcac taccctcccc
    15781 acaagggagc gaccacgggc agcggctatg gcatcccgct tgaggatagc catgccatgc
    15841 tccgggcgat ggaggaaact ggcgtaccgc tgctcggaca cttcgagacc gtgtacgacg
    15901 cttccgggcg cgagcttcca tcgcaccagc gggaagcgca catggtggac acggtgctct
    15961 ggcgcctgcg cgacaagtac ccgaatcttc ggatctgctt cgagcacgcg agcacccagg
    16021 aggcggtcga attcgtcgaa gcggatacga gcggcaggac ggtcatgacc gtcacgccgc
    16081 agcattcgct gttcacgaag gccgacttcg aggagcacgg caccgacctc aaatgtatgc
    16141 cgatcgtgaa gacgccagaa gatcgcgctg cgatcgtcga cttcatcacg agtggcgacc
    16201 gtcgcgcgat cgccggagac gacactgcgc cgcatctgtc gaagaccaag cgcacggcct
    16261 tcgaggacgc ggcgaacggc tgctggcttc cccacgcgat cggactgtac gcgacggcgt
    16321 tcaatcgtgc cggtgcgctt gatcaccggt tcgagcggtt tatgtcgctc aacggtccgg
    16381 agtggtgggg gcttcccgca cctgacggac aagatgtcat caccctcgtt cggaccagtt
    16441 ccagaattcc ggaacccact cgggtaccgg aagaggacga cgtcgtggtt ccgctcggct
    16501 ggaaagggac acctgaccag atgcgagttg aattcaccgt caagtaatga ggtacgggcg
    16561 caggaggatc tcctgcgtcc gttttcatgt gtgcgtccac caggactcga acctgggacc
    16621 accgaggtat aagctcgggg ctctaaccaa ctgagctatg gacgcggggt aactataccg
    16681 ctcggagaac gcgtgcgcca tgcgcaatga tttggtaagg tgcggctagg tgtaacaaaa
    16741 ggattactga ttatgtcctc acggatcttt gctccggtct gggtgattcc agcattcttc
    16801 ggacagaagg gtcaggggtg ggccatcttg gcagtgatag agctcctgat tttccttctg
    16861 ggcgcaagga tggcgttctg gccccaggga gatcttctgg gctctgagcg cttcatcgtt
    16921 ctgggcatcg cggcggtgat ctacgtcatc ctggcgatca tctactaccg cacacccacc
    16981 ccgatccgga caccctaggc ccttacggcc tggaaaagaa gaacggcgac cctcctgggc
    17041 cgccgttttc tcttttactt atgtcctgcc ccgactgcag gcgccgagat ctcctcttca
    17101 ggctcgggtg ccttcggcgt gatgccattt gcgatgagga tcttctcgaa ctcttcgcgc
    17161 tcgatggtct ccacctcgac gagcttctcc gcgatcgcat cgagcgcacc gcggtgctgc
    17221 tcgagcactt cgcgcgcacg acccttcgct tcctcgatga tacgcgctac ttccgcgtcg
    17281 atcttgccgg ccacttcctg tgagtacgcg gcgtctccgt agcggtccgc agcgtacgct
    17341 accggaccga gcgaatcgct catgccgtag cgcgctacca tatcgcgcgc gagcgccaca
    17401 ataaccgcaa ggtcgttcga gggaccggtc gtgagatcgc cgaagatcat ctcttctgcg
    17461 acgtagccgc ccaaggttcc tgcgatgtcg tcgaggaatg actttctcga ctgcatcttc
    17521 ttatcgtcaa acgggagatt gagcgtatag ccggctgcac ggccgcggct gatgatagag
    17581 atcttgtgga tcggatccgc atgctcgagc acggaaccca cgagcgcatg gcccgcctca
    17641 tggtacgccg taacacgctt ctccttcttt ccgaggatat ggctctttcg ctccggtccg
    17701 agcatcacct tctcgataga gcggatgagg tcgtactgtg tgatctcctt gcgattttcg
    17761 cgagctgcga gaatcgcacc ttcattcatg agcgaataaa gttcggcgcc agagaagccc
    17821 ggggtacgct ccgcgataac ctcaagattt acatccgggc caagcggctt cttgcgcgca
    17881 tgcacaccta ggatctcgag acggtccttg cggtccggaa ggtcgatggt cacgcgacga
    17941 tcgaagcggc ccggacgaag aagcgccgga tcaagcacgt caggacggtt cgtcgccgcc
    18001 ataacgataa ccttctccgt cggatcgaat ccatccatct caacgagaat ttggttgagc
    18061 gtctgttcgc gctcgtcgtt gccgccaccg attcccgtgc cgcggacgcg tcccacggca
    18121 tcaatctcat caacgaagat gatcgccgga gaggtgcgct tcgctaggag gaagaggtcg
    18181 cgcacgcgtg aggcacccac acctacgaac atctccacga actcagaacc tgagatagag
    18241 aagaacggta cgcccgcctc gcccgcaacc gcgcgcgcaa gcaaggtttt tccagttccc
    18301 ggagcaccca tgaggaggat tcccttcggg atacgagcac caatgtcgag gaacttcttc
    18361 gggttcttaa ggaaatccac gatttcaagc aactcctcct tagcttcctt ggcgcctgcg
    18421 acgtccgcga acattacgcg ctgcgaggtg tccgccggat cgatcatgcg cgccttcgac
    18481 tgaccgaagg agaacgcctg catgccggcg cctcgcacct ggcgtgtgag gaaccagaag
    18541 agcaccgcga ggaagaggat cggaaggagg atcggcgcga ggttgaagaa ccagtatgcg
    18601 aacccgctct ccttctcgat ggaggtggac accacggaga gctgctctgg agtaacgccg
    18661 tatgcggaaa gggtctcagg aagcgatgct tctgggtcct tcatagaggt cttcttcgtg
    18721 ccgtcctggt acgtgagtgc cacctcgttg ccgctgatct ccatcgcgga caccgagccc
    18781 gccttcacat cagcagctac ctgcgaaagc gagatctgct cgggctttgt gctctctccc
    18841 ataaggagcg agtagattcc ggtgaggaca atgaggataa ggatcgttga tataacgttc
    18901 gccatgaatc ccccgccctt aggcatcggg accttccctt tcggcagttt cgggccgccc
    18961 ttcttagacg ctttcaacgc gggtttttta gtaggtgcca tagtcgctac agtatacgcg
    19021 agcagcgcat ttgccgaaat cctgccggct caaagcgact accgtatgtt tactaccacg
    19081 tcgcgcgcag aactctcgcc gatgaagcct tcgaacaggt ggagattgtc gagattcctg
    19141 agaatgatga tgaccgagat aagtgacacc acgaatgcgg acttgagcat cgatccaagc
    19201 agcatgccaa tagacggaat aacgctaatc gacatgagaa gcatcagaag gaagaatacg
    19261 atcaagaacc actggaaaga cgggatgcgc tcctgataca gcatcaccat attcttgcgg
    19321 agcacctggc agtccgagag gcccgtcatc atgcgaccga tagcctggtt cctaagtgac
    19381 tctactttct ctgaaccgat tcggttctca agaacgcgat gtatcgacga aatcacgttc
    19441 gtctcctggg cgaaatgcgc ttcccatgta cgagccttaa gtatcccttc gtaatgatcc
    19501 ttgatgatct ttccggtctc aacctcaact tcagggctta cgttaagcga cgcacgataa
    19561 acactcgaga gcttgccatc gaaggtggcg atcgtctcgc gcagcttgct gtagcgcgct
    19621 ccctgtcggg aaatgaagaa tcccgtgaat atggagaaga agaatgtgct gatcgttatg
    19681 aagatcctat cggtattctc cgccccgaac gagacgtagt agtacaccgc ggtcatggca
    19741 aggaagacac cgaacaggat gatttcagtc tttcgtacgt tcatgaagac cagtatacat
    19801 gccacttacc gcctctttgg agaagcgtat ttaggaaaat cctgctacct tttataggtc
    19861 atggctcccc tactggagaa taagaaggtg cggctccgtt tcgagtcgct tgagaccttt
    19921 actgccggaa tcgaactctt cgggttcgag gttaagtccc tgcgcgcaaa actcggcagc
    19981 cttgagggtt cgcgtgtcgt ggtgcgcggc ggcgaagcct tcctcactgg catgacgatc
    20041 ccgccgtacc agatggcgaa cgcgcctaag gactacgatc cagagcgcac tcgtcgcctg
    20101 ctcctttctc ggaaggaaat cgctgagctg ctcgatgcgg aatcgaagaa agggttgaca
    20161 attgtgcctt ttgaagtgta taataaaggg agatatctga aggtgcgtgt cgatattgtg
    20221 cgtggcaaga acaaagctga cagacgcgag gacatcaaac gtctggaagc gaagaaggaa
    20281 gccgaccgtg caatgcgagc caagtag
//
LOCUS       DASWXU010000031         4492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_23187, whole genome shotgun sequence.
ACCESSION   DASWXU010000031 DASWXU010000000
VERSION     DASWXU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23187"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1325
                     /gene="secD"
                     /locus_tag="VGE48_01115"
     CDS             27..1325
                     /gene="secD"
                     /locus_tag="VGE48_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY1042006.1"
                     /translation="MRYIPALVVLVVSGLLGWYVLSPGHPVKLGLDLAGGTELIYRAD
                     TSLVQTDTQGALNSLREVIERRVNVFGVAEPLVQIEQSSAVAGAVENRLLVELPGVTD
                     VQAAVDAIGKTPSLEFRLLDPASMIGGEPAFVPTELTGRYLKKADLQFGSQSGAPGIS
                     NEPIVLLTFSDEGAALFEQITRDNVGNMLAIYLDGEPISTPVIREAIPGGVATVSGGF
                     TPEEARELVRNLNFGALPVPIELIGSGTVGPTLGAEAFEEGLVATGIGFALVALFMIL
                     WYRLPGLVASLALVIYVVATVALIKFIPITLTTSGIAGFILSIGMAVDANILIFERMK
                     EELRRGVAAREALRIGFSRAWTAIRDGHFTMIISSVILFWVGTSIVQGFALVFGLGVI
                     ASLITAVFITRVLLLTIVPEEAGKTWRFLLETGLYRHNHK"
     gene            1327..2250
                     /gene="secF"
                     /locus_tag="VGE48_01120"
     CDS             1327..2250
                     /gene="secF"
                     /locus_tag="VGE48_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY1042007.1"
                     /translation="MFVVRHRRFFFILTGLLLVGSVIATAVIPPSLSIEFTGGSLMEV
                     RYEAERPSVETIRERLEPLHLGEPSVRESGESAITLRTRTLSPEERTAVMSALSSAGT
                     ELVTELRFNSIGPSLGNELAIKALYAIGVVTLAIMLYIAWAFRGISRPVPSWGYGIIA
                     VFMLAIDIIVPTGFYAVYAYFTGAQFDSLFVIALLALLGYCINDVIVIFDRVREHLRN
                     NEADEIEEDFEVTVGKSIDETMGRSINTGLTVVLALLALTVIGSEATRNFALVMMIGV
                     AAGTFSSITRSAPLLIPIAAWLEKKRAAKEA"
     gene            2256..2810
                     /locus_tag="VGE48_01125"
     CDS             2256..2810
                     /locus_tag="VGE48_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1042008.1"
                     /translation="MIIGITGSFGAGKGAVVDYLVREKGFADYSARAFITDEIKFREL
                     PVNRDTMAEVANDLRQKHEPTYIIESLYTKAKDRGGDAVIESLRAVAEVRLIKKLGGV
                     VLGIDAPPELRYERAVARGSETDKVPYEEWLQQEIRESNPNDPTKQDIFGSLREADIV
                     IENKGSLQELYEKIDEMLKQQRLG"
     gene            2822..3286
                     /locus_tag="VGE48_01130"
     CDS             2822..3286
                     /locus_tag="VGE48_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1042009.1"
                     /translation="MPHIHEKIDYAGDVFIVNDGAVLLRMHDKYDMWLPPGGHVELDE
                     DIVEAAVREAKEETGLDVTLLGTAPEAVDEGERELLVPRFINRHPVSDTHEHISFIYF
                     GISSTRDLAPAEGEKTDGFKWFTKEDLVDPDNGVTARVRQYALAALEAAATE"
     gene            complement(3276..3986)
                     /gene="pyrH"
                     /locus_tag="VGE48_01135"
     CDS             complement(3276..3986)
                     /gene="pyrH"
                     /locus_tag="VGE48_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY1042010.1"
                     /translation="MRLPRRNKREYVVVSVGGSLLVPDGIDVSFLTAFRSLLRKKTQD
                     GSSFYIVAGGGRLSRNYQEAAAAIRGEEELDREDMDWLGIHSTRLNAHLLRTIFIEDA
                     QARIIKNPTHRFRGRESIVIGAGWKPGWSTDYCAVMAAKTLGAKKLVNLSNIDYVYTA
                     DPRKNPDAQKIEKITWADFRKLIPEEWDPGLSSPFDPVAAREAEALGLEVAIINGAML
                     PEFENYLDGKPFTGTVIR"
     gene            4137..4352
                     /locus_tag="VGE48_01140"
     CDS             4137..4352
                     /locus_tag="VGE48_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042011.1"
                     /translation="MTEREFCLHGKADAGRPVRHWFDAQDPEAAIKKATSYVLDHGLE
                     FDKLERYARRRRRGKKDITIIPVPELE"
BASE COUNT          919 a         1235 c         1293 g         1045 t
ORIGIN      
        1 tagactctga gtaatacatc atttatatgc gctatatacc tgctctcgtg gtgctcgtcg
       61 tgtctggcct tctcggctgg tacgttctct ctccggggca cccggtgaag ctcggtcttg
      121 accttgcagg tggtacagaa ctcatctacc gtgccgacac cagtctcgtg cagactgata
      181 cacaaggcgc ccttaattcg ctccgcgaag ttatcgagcg tcgtgtgaac gtgttcggtg
      241 ttgcagaacc gttggtgcag atcgagcagt cgagcgcagt tgcaggagca gttgaaaacc
      301 gtcttctcgt ggagcttccg ggcgtcacgg acgtacaggc cgcagtagac gcaatcggaa
      361 agacgccgtc cttggagttc aggcttctgg accctgcgtc catgatcggg ggcgagccgg
      421 cgtttgtgcc gaccgagctc accggccggt acctcaagaa ggcggatctc cagttcggca
      481 gccagagcgg ggcaccaggt atttcaaacg agccgatcgt gctccttacc ttctcagacg
      541 agggtgccgc gctcttcgag cagatcactc gcgacaatgt gggtaacatg ctcgcgatct
      601 atcttgatgg cgaaccgatc tccacgccgg tcatccgcga ggcgattccg ggaggcgtag
      661 cgaccgtatc cggtggcttc acgccagaag aagcacgtga gctcgtgcgc aacctcaact
      721 ttggtgcgct tccggtcccg atcgagctca ttggttcggg cacggtgggt ccgacgctcg
      781 gcgcggaagc cttcgaggag ggtctcgtag cgaccggtat cggctttgcg ctcgtggcgc
      841 tcttcatgat cctctggtac cgcctcccgg gccttgtcgc gtcgctcgcc ctcgttatct
      901 acgtggttgc aacggtggcg ctcatcaagt tcatcccgat tacgcttacg acttcaggta
      961 tcgcgggctt catcctctct atcggtatgg ccgttgatgc gaacattctt atcttcgagc
     1021 gtatgaagga ggagcttcgt cgtggcgttg ctgcgcgcga ggcgctccgc atcgggttct
     1081 ctcgcgcctg gactgccatc cgtgacgggc atttcacgat gatcatctcg agcgtgatcc
     1141 tcttctgggt aggcacctca atcgttcagg gcttcgcgct cgtgtttggc ctcggtgtga
     1201 tcgcatcctt gatcacggcc gtgttcatca ctcgagttct cctgcttacg attgttcctg
     1261 aggaagcagg gaagacctgg cgcttcctgc ttgagaccgg gctctatcgc cataaccaca
     1321 agtaatatgt ttgtcgtacg ccaccgccgc ttcttcttta tcctcacggg ccttcttctt
     1381 gtaggttccg tcatcgcgac cgcggtcatc ccaccatcct tgtcaatcga gttcacgggc
     1441 ggctccctca tggaggtccg ctacgaagca gagcgtcctt cggtagagac gattcgtgag
     1501 cgcctcgagc cgcttcacct gggcgagccg tcagtgcgcg agagtggtga gagtgctatc
     1561 acgcttcgta cccgcacgct ctctccggaa gagcgcactg cggttatgtc tgcgctctca
     1621 agtgcaggta ctgagctggt aacggaactt cgtttcaatt ccatcggtcc ctcgcttgga
     1681 aacgagctcg cgatcaaggc gctctacgct atcggtgtcg tgacgctcgc catcatgctg
     1741 tacatcgcct gggcattccg cggcatctcg cgtccggtac catcctgggg ctacggcatc
     1801 atcgctgtgt tcatgctcgc gatcgatatc atcgtgccga cgggcttcta tgcagtctac
     1861 gcgtacttca cgggcgcgca gttcgattcc ttgttcgtga tcgcactctt ggcgcttctt
     1921 ggatactgta ttaacgacgt gatcgttatc ttcgaccgcg ttcgtgagca tctccgtaac
     1981 aatgaagcgg acgagatcga agaggacttt gaggttacgg tcgggaaatc aattgatgag
     2041 actatgggtc gctccattaa caccggtctt acggtggtgc tcgcactcct tgcactcacg
     2101 gttattggaa gcgaggcgac ccgcaatttc gcgctcgtca tgatgatagg tgtggcggcg
     2161 ggtacgttct cctcaattac gcgctccgct ccgcttctca ttccgatcgc tgcgtggctt
     2221 gagaagaagc gcgccgctaa ggaagcctag ggatcatgat tataggcatc accggttcgt
     2281 tcggagcagg aaagggcgcg gtggtggact atctcgtgcg cgagaaggga ttcgcggact
     2341 attccgcgcg tgccttcata accgacgaga ttaagttccg agagcttccg gtgaatcgcg
     2401 acaccatggc agaggttgca aacgacctcc gacagaaaca cgaacctact tacatcattg
     2461 aatctttgta taccaaggcg aaggaccggg gaggagatgc ggttatcgag agcctccgcg
     2521 ctgttgctga agtgcgtctc atcaagaagc tcggaggtgt cgtgcttggc attgatgcgc
     2581 cgcctgagct tcgctatgaa cgtgcggttg cgcgaggaag cgagactgac aaggttccgt
     2641 acgaggaatg gcttcagcag gagatccgtg aatcgaatcc gaacgatcct acgaagcagg
     2701 acatcttcgg ctctcttcga gaggcggaca ttgttataga gaataaaggt tcgcttcagg
     2761 agctttatga aaagatagac gaaatgctta agcagcagcg tcttggataa tacctaaatc
     2821 tatgccgcat attcacgaga agattgatta tgcgggtgac gtatttatcg tgaacgatgg
     2881 tgccgtgctc ctgcgcatgc atgacaagta cgacatgtgg ctgccgccgg gagggcacgt
     2941 agaactcgat gaggatatag ttgaggcagc ggttcgggaa gcaaaggagg agactggcct
     3001 cgatgtaacc ctgctcggta cggctccaga agcggtggac gaaggtgagc gcgaacttct
     3061 cgtgcctcga tttataaata gacatccggt ttcggataca cacgagcaca tctcgttcat
     3121 ttattttggc atctcttcca ccagggatct cgcgccagca gaaggagaga agacagacgg
     3181 gttcaaatgg tttaccaaag aagatctagt cgaccctgat aacggtgtta ctgctcgagt
     3241 acgtcagtac gcgctcgcag cgcttgaagc agcggctacc gaataaccgt tccagtaaac
     3301 ggttttccgt cgagatagtt ctcgaactcc ggcaacatcg cgccgttgat gatcgcaacc
     3361 tcaaggccga gcgcttctgc ttcgcgcgcg gcaaccggat cgaacgggga agagagcccc
     3421 ggatcccatt cttcagggat aagcttcctg aagtcagccc aagtaatctt ctcgatcttc
     3481 tgtgcgtctg ggttcttgcg agggtccgca gtgtatacgt aatcgatatt ggagaggttc
     3541 acgagcttct tcgcacccag cgtctttgcg gccataacgg cgcagtagtc cgtagaccag
     3601 cctggcttcc agccggcacc gataacgatc gattcgcgtc cgcggaagcg gtgcgtcggg
     3661 ttctttatga tgcgtgcctg tgcatcctcg atgaatatag tacgcaggag atgtgcgttg
     3721 agacgggtgg aatgaatgcc aagccagtcc atgtcctcgc gatcgagttc ttcttcgcca
     3781 cggatcgccg ctgccgcttc ctgatagttt cgcgagaggc gtccgccacc tgcgacgata
     3841 taaaaagaag acccgtcctg ggtctttttg cggagtagcg aacggaacgc agtaagaaag
     3901 gagacgtcga tgccatcggg aacgaggagc gagcctccga cggagactac gacatactca
     3961 cgcttgttcc ttctaggcaa acgcatgcaa agcttgacgg ctcctctcat tctacgctca
     4021 tgagtgggga gcgcaatctg gaaaagttca gctttgatgt catagcgact tctcttgacc
     4081 gaatggatca caaagagcta taaccagccc tagaccatca atcagggagt gagacgttga
     4141 ctgagagaga attttgtctg catggcaaag ccgacgcagg acgcccggtg cggcactggt
     4201 tcgacgcgca ggaccctgag gctgcgatca agaaagccac gagctatgtg ctcgaccacg
     4261 ggctcgagtt cgacaagctc gagcggtacg ctcgcaggag aaggcgcggc aagaaggaca
     4321 tcacgatcat tcccgtgccc gaactcgaat agggcagacc cgccgcgact aaccctcgcg
     4381 gcggccttat tttaggccct atattccggc ttgacgctca tttccaggtc ctgtactata
     4441 gccctactgc ctccttgtgg aggcggggtt tgttttcgct cttcgaaaac tg
//
LOCUS       DASWXU010000032         9351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_484867, whole genome shotgun sequence.
ACCESSION   DASWXU010000032 DASWXU010000000
VERSION     DASWXU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484867"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1747)
                     /gene="thrS"
                     /locus_tag="VGE48_01145"
     CDS             complement(11..1747)
                     /gene="thrS"
                     /locus_tag="VGE48_01145"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY1042012.1"
                     /translation="MASLEEKRHTLAHLLAAAVLERYPHAKATIGPAVDTGFYYDFDF
                     SGGDAPKEADLAKLQEAMEELLPTWTSMSGIEVSEKDARDRFKDNPFKLEIIDGIREA
                     GDTITLYTAGAFTDLCRGGHSEKPAHDIKPGSFVLERIAGAYWRGDETRPMLTRIYGL
                     AFDTKEELDAYQAQQDEARKRDHRKLGAELKLFTLSPLVGSGLPLLQPKGALIRREIE
                     EYLWELHKDRGYDRVWTPHLAKEDLYVTSGHASKFGDELFRVSGKEEKFFMKPMNCPH
                     HMQIFGDNQFSYRQLPVRYFEPATVYRDEKTGQLAGLTRVRAITQDDGHLFCRASQIG
                     EEVGAIVSIIKSFYGTMKMLDGYWVRLSVRGPEGQYLGSDEVWEKAEAALEDAAKENE
                     LPYERIEGEAAFYGPKLDFMFKDAIGREWQLATIQCDFNLPERFNLEYTNEEGQKERP
                     VVIHRAISGSLERFMGVMIEHFAGAFPTWLSPEQVRVLPVSEKHEAYADNVLDALKAA
                     GVRAVLDAGDSLGKRIRNAKQQKVPYVLVVGDKETEAGSVSVESRDRGQEGSLTLDEF
                     VSRIGTEIGERQ"
     gene            complement(1814..2077)
                     /locus_tag="VGE48_01150"
     CDS             complement(1814..2077)
                     /locus_tag="VGE48_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042013.1"
                     /translation="MPETLASSDLRAILKACDPQNGLFPAAPLLAPGLSLIAIGLQSV
                     EAALDQVVDRDERLPPELEALVTRFYDRGTTAYATLHARTQGL"
     gene            complement(2122..5265)
                     /gene="dnaE"
                     /locus_tag="VGE48_01155"
     CDS             complement(2122..5265)
                     /gene="dnaE"
                     /locus_tag="VGE48_01155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY1042014.1"
                     /translation="MPGFVHLHAHSHYSFLQALPKVPELVKAAKAEGMDAVGLTDAGN
                     LHGAVEFYKQCEKQGVKPVFGVDAYVAPVSRHDRDHNEKRARIVLLARNNEGYENLMK
                     LVTRSFLEGFNERPRMDREILREHKDGLIALLPSFASDVALAFKAGDPKGAAAALAEY
                     QSIFGTDNVYLEISHHPRVDGHEKRMQEIKQLAKDSGAPLVAQHDVYYLKPDDREATE
                     VLRRIGQGERGRNEDEDFSFVSEKQMREWFKDVPEAVDESGRIADRCSVTMELGKWNF
                     PKVDVPEGSTHADELRKRTYAGISMRGMQQTEEVEKRIEYELDVIISKGFAPYFLAVS
                     DLLTFAKSAGIMSTTRGSAAGSLVSYLNGITNVDPLFYKLPFERFLNPERPKAPDIDM
                     DFADNRRDEMIAYAREKYGENNVAQIGTFGTLMARAAVRDVSRALGHAYRTGDRIAKL
                     IPMGSQGFPMTIERALELEPELKQLYDEDDDVRQIVDLAQRIEGCVRHTGVHAAGVVI
                     APTPLWEWTPVQFDPKNPEKRITQFDMHAVEDAGLLKFDFLGIKNLAILSDAVERVRE
                     TRDIWIDIENIPLDDAKTYAMLARGETEGTFQLNGSGMTRYLKELQPTTIHDINAMVA
                     LYRPGPMETIPQYIERKKNPRLVSYMDERMKDYLDASYGLLVYQDDVLLTAITLGGYS
                     WLEADTLRKAMGKKIPAEMEAQKEKLLKGFKEYGKLSPEKAAKIWQLIEPFAAYGFNK
                     AHAACYGKVAYQTAYMKANYPVEYMAALLTADAGDTEQIAILVAEAERLKIPVLPPDI
                     NESGADFTVAGEDKDTVRFGLTSIKNFGEGISRAIIEERDRGGSFRDLSDFLARVGSK
                     NLNKKSLESLIKSGAMDSMGERSHLLSHIELLLTFHKEATATAAQDSLFAFAAPTLTL
                     PPAHGETSLTDKLSWEKELLGIYVSGHPLDAHTDKTAKAGTTIAQIKEEPKPGFTVIL
                     PVLVDEVKLFLTKKGEKMAFVKFSDKTDSIEGVIFPKVFHEKSGIVVPGNCLLVKANI
                     SGRNGETSLSVENAKLL"
     gene            5314..5799
                     /locus_tag="VGE48_01160"
     CDS             5314..5799
                     /locus_tag="VGE48_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1042015.1"
                     /translation="MAEKRGFTLIELLVVIAIIGILSSVVLASLNTARGRARDSSRQS
                     ELRQMQTAMQAYYLQTGNYPGNPTPGSATYSNSPNWQTIWQPIIDAGFIGSAPTGPTG
                     SDYRYFTYGAGSTQGAMLVTVLEAASPSNGYPGTCRPFSGVNWCRSDIQTREWCLCNP
                     Y"
     gene            5804..6388
                     /locus_tag="VGE48_01165"
     CDS             5804..6388
                     /locus_tag="VGE48_01165"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY1042016.1"
                     /translation="MIVVGVDEAGRGPLAGPVAVGAVAAEEGFDILAAFPGLNDSKRL
                     SEKKREALFVLLQQAIRDGHIRATVTLMSVKEIDGEGVAHAVRHGVWKGVRKLLPNPE
                     DGKVWLDGSLKAPPEYEQDTVIGGDGIVPAIMLASVAAKVTRDRYMQKLDATYPLYGF
                     AVHKGYGTKVHREAIREHGPCEAHRKSFLRNVLK"
     gene            6767..6937
                     /gene="rpmF"
                     /locus_tag="VGE48_01170"
                     /pseudo
     CDS             6767..6937
                     /gene="rpmF"
                     /locus_tag="VGE48_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
     gene            7196..8089
                     /gene="nusB"
                     /locus_tag="VGE48_01175"
     CDS             7196..8089
                     /gene="nusB"
                     /locus_tag="VGE48_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY1042017.1"
                     /translation="MANRHLARSVVLQTLFEWDTSRLSDGEATDALQRNVEEFGGDDT
                     DRPFMETLLAGVLAKRADLDLVITKAAPDWPLERIAPVDRNILRIGLYELLFADRTQV
                     PAKVAINEAIELAKVFGGDSSGRFVNGVLGAVYKELGEPGKDEKGKKDRVRREDLPVE
                     ELGGAIVYAYHEGQYYLALVHDVFGHWTLSKGKIPEGMSVEEGTVDGLKDELGLPAVI
                     KTKIGENEYVASHPEKGKTRKHVSYFLAESPYMELTLEKKGGLDDARWFRLTDILELN
                     FYDDMLPIVTEAVQKLLDTKR"
     gene            8122..8802
                     /gene="rnc"
                     /locus_tag="VGE48_01180"
     CDS             8122..8802
                     /gene="rnc"
                     /locus_tag="VGE48_01180"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009441668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY1042018.1"
                     /translation="MSDFSAHAAALGVSFKNLDLLVEALTHRSYLNEHRTAGNHNERL
                     EFLGDAVLELAVTRFLFERFPTKPEGDLTAYRAALVNTVSLAETSEALGVNDMLLLSK
                     GERKDTGRARENILANAFEAILGAIYLDQGYESAEAFVANHLYPKIDAILATRSWQDG
                     KSHFQEVAQEKRGITPTYKTIREEGPDHDKHFTVGVFLGAEEVARGEGKSKQEAEQAA
                     AQAALDIF"
     gene            8865..9209
                     /locus_tag="VGE48_01185"
     CDS             8865..9209
                     /locus_tag="VGE48_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042019.1"
                     /translation="MNRFAMYWLLITMGLVFAATYAASVVYPLGNVVMWTAVVGGALA
                     GIAACVYGREISESRFIITLLFALLVVFLLSDAYNPGFNPEAAVKLGIALTVASLFAL
                     GWTIGNLIRKPE"
BASE COUNT         1898 a         2630 c         2772 g         2051 t
ORIGIN      
        1 ctatcagttc ttactggcgt tcgccaatct cggtcccgat gcgagataca aactcatcaa
       61 gagtgaggga accttcctgc cctcggtctc ggctttcgac cgatacgctc ccggcttcag
      121 tctccttgtc tccgacgaca aggacgtacg ggaccttctg ctgctttgca tttcgaatac
      181 gcttcccgag cgagtctcct gcatcgagta cggcgcgcac acccgctgcc ttgagcgcgt
      241 cgagcacgtt gtccgcgtac gcctcgtgct tctcggatac cggaaggaca cgcacctgct
      301 caggagagag ccaggtaggg aaggcacctg cgaagtgctc gatcatgacg cccatgaagc
      361 gctcgaggga gcctgagatc gcgcgatgga taactaccgg gcgctccttc tgaccctctt
      421 cgttcgtgta ttcgaggtta aagcgctctg ggagattaaa gtcgcactgg atggtagcaa
      481 gctgccattc gcggccgatc gcgtccttga acatgaaatc gagcttcggt ccgtagaacg
      541 cggcttcacc ctcgatgcgc tcatacggga gctcgttctc tttagcagca tcctcaagcg
      601 cagcctctgc cttctcccac acctcatcag accccaagta ctggccctcg gggccgcgga
      661 ctgagagacg cacccagtac ccgtcgagca ttttcatggt gccgtagaag ctcttgatga
      721 tggagacgat tgcacctacc tcctcgccaa tctgggacgc gcggcagaag aggtgcccat
      781 cgtcctgggt gatagcacgc acgcgcgtga ggcctgcgag ctggccggtt ttctcgtccc
      841 gataaacggt cgccggttca aagtagcgca ctgggagctg gcggtaggag aactgattat
      901 ctccgaagat ctgcatgtgg tgcgggcagt tcattggctt catgaagaat ttctcttctt
      961 tacccgatac gcggaacagc tcatcgccga acttagacgc atgtccgctc gttacataga
     1021 ggtcttcctt agcaagatgc ggcgtccaga cgcgatcgta accacgatcc ttatgaagct
     1081 cccagaggta ttcctcgatc tcgcgtcgga taagggcacc ctttggctgg agaagcggaa
     1141 ggccggaacc tacgagcggg gagagcgtga agagtttaag ttccgcaccg agctttcggt
     1201 gatcgcgctt ccttgcttcg tcctgctggg cctggtacgc gtcaagctct tccttcgtgt
     1261 cgaacgcgag tccgtagatg cgcgtgagca taggcctggt ctcgtcgccg cgccaatatg
     1321 cgcccgcgat gcgctcaagc acgaacgagc ctggcttgat gtcatgcgcc ggcttctcgc
     1381 tgtgccctcc gcgacagagg tccgtaaaag cacctgcggt atagagcgta atcgtatctc
     1441 cggcttcccg gatgccgtcg ataatctcga gtttaaacgg attgtcctta aatcggtctc
     1501 gcgcatcctt ctcggacact tcaattcctg acatgctggt ccaagtcgga aggagttctt
     1561 ccatcgcctc ctggagcttt gcgaggtctg cttccttggg cgcgtctcct cctgagaaat
     1621 caaagtcgta ataaaaaccg gtatcaaccg cagggccaat ggttgctttt gcgtgcgggt
     1681 atcgttcaag cactgcagcg gcaagcaggt gagctagggt gtgacgcttt tcttcaaggg
     1741 aagccatatg cagggaggat agcatgaaac gggtgacggc ggctctgtag ggagccgccg
     1801 ttcaatggat gcttcacaag ccttgggttc gtgcatgaag ggtcgcgtag gccgtggtgc
     1861 cccgatcgta gaaacgggtc accagggcct cgagctcagg cggaagccgc tcatctcgat
     1921 cgacgacctg gtcgagcgct gcttcgacag actggaggcc gatggcgatc agcgatagtc
     1981 ctggagcgag caggggagct gcgggaaaga gcccgttttg tgggtcgcaa gcctttagta
     2041 tggcccgcag gtcggaggag gcgagggtct cgggcatgta aggctccttt cagccaaggt
     2101 tcgataccgt tccttcttat atcacagcag cttcgcgttc tctacggata acgaagtctc
     2161 tccattccta cccgatatat tcgccttcac gaggagacag ttgcccggga ccacgatgcc
     2221 actcttctca tggaacactt tcgggaagat gacaccctcg atgctgtcgg tcttgtcgga
     2281 aaatttcacg aatgccatct tctcaccctt cttggtaaga aagagcttta cctcgtctac
     2341 gagcacaggg aggataaccg tgaagccggg cttcggctct tcctttattt gggcgatggt
     2401 ggtgccagcc tttgcagttt tgtcggtgtg cgcgtcaagc gggtgtccgg atacataaat
     2461 acccagcagc tctttttccc aggagagctt gtcggtgagg gaagtttcgc cgtgtgcagg
     2521 aggaagcgtg agggtgggtg ctgcgaatgc aaagagggaa tcctgtgctg cggtcgccgt
     2581 tgcttcctta tggaaggtga ggagcagctc gatatgggaa agaagatgcg agcgctcgcc
     2641 catggaatcc atggctcctg atttaatcaa agactcgagc gacttcttgt tgaggttctt
     2701 cgagccgacg cgcgcgagga aatccgagag gtcgcggaag cttcctccgc gatcgcgctc
     2761 ctcgatgatg gcgcgcgaga tgccttcgcc gaagttcttg atcgaggtga gtccgaagcg
     2821 caccgtatcc ttatcctctc cggcaaccgt gaagtccgca cctgactcat taatgtctgg
     2881 cggaagcacc gggatcttga ggcgctcggc ttcggctacc aggatcgcga tctgttcagt
     2941 gtctccggcg tcagccgtga ggagcgcggc catgtactcg accgggtagt tcgccttcat
     3001 gtaggcggtc tggtacgcca cctttccata gcaggcagcg tgcgccttgt tgaatccgta
     3061 cgcggcgaac ggctcgatga gctgccagat cttcgcagcc ttctccgggg agagcttgcc
     3121 gtactccttg aagcctttga gaagcttctc cttctgcgct tccatttctg ccgggatctt
     3181 cttacccatg gccttacgga gtgtgtctgc ctcgagccat gaatagccgc cgagcgtgat
     3241 agcggtgagg agcacgtcgt cctggtatac gaggagtccg taggacgcat cgaggtagtc
     3301 cttcatacgc tcatccatgt aactcacgag ccggggattc ttcttgcgct cgatgtactg
     3361 tgggatggtc tccatcggtc ccgggcggta caaggccacc atcgcgttaa tgtcgtggat
     3421 agtggttggc tggagctcct tcaggtagcg ggtcatgcct gagccgttga gctggaaggt
     3481 gccttccgtc tcgccgcgcg cgagcatggc ataggtcttc gcgtcgtcga gcgggatgtt
     3541 ctcgatatcg atccagatgt cgcgcgtctc tcgtacccgc tcaaccgcgt ccgaaaggat
     3601 ggcgaggttt ttgatgccga ggaagtcgaa cttaagcagg cccgcatcct ctaccgcgtg
     3661 catatcgaac tgcgtgatgc gcttctcagg gttcttcgga tcgaactgca ctggggtcca
     3721 ttcccacagt ggcgtaggcg cgataaccac gcctgccgca tgcacgcccg tgtgtcgtac
     3781 gcagccctcg atgcgctgtg cgaggtccac gatctgacgc acgtcgtcat cctcgtcata
     3841 gagctgcttc agctccggct cgagctcgag cgctcgttcg atcgtcatag ggaagccttg
     3901 gctacccata ggaatgagtt ttgcgatgcg atctccggtc cgatacgcgt gtccgagtgc
     3961 acgtgatacg tctcgcacgg ccgcacgcgc catgagggta ccgaaggtgc cgatctgcgc
     4021 aacattattc tcgccgtact tctcacgcgc atacgcgatc atctcgtcgc gcctgttgtc
     4081 ggcgaagtcc atgtcgatat ccggtgcctt cggacgctct gggttgagga atcgctcgaa
     4141 gggaagctta tagaaaaggg gatcgacgtt cgtgatgccg tttagatatg agacaagcga
     4201 gccggctgcg gaccctctcg tggtggacat aattcctgca gatttcgcga aggtgaggag
     4261 gtcggacacc gcgaggaaat acggggcgaa gcctttggag atgatgacgt cgagctcgta
     4321 ctcgatgcgc ttttcgactt cttcggtctg ctgcatgccg cgcatagaga tgcctgcata
     4381 cgtgcgcttt cggagttcgt ccgcgtgcgt gcttccttcc ggaacgtcca ctttggggaa
     4441 gttccacttt ccgagctcca tagtcacgga acagcgatcc gcaatgcggc cggactcgtc
     4501 cacggcttct gggacatctt tgaaccattc acgcatttgc ttttcggaga cgaaggaaaa
     4561 gtcctcatct tcgttcctgc cgcgctcgcc ttgtccgata cggcgcagca cctcggttgc
     4621 ctcacgatcg tctggcttta ggtaatagac gtcatgctgc gccaccaggg gtgcaccgga
     4681 atccttcgcg agctgcttga tctcttgcat ccgcttttcg tgtccgtcca cgcgcgggtg
     4741 atgtgagatc tcaaggtata cgttatcggt accaaagata ctctgatact ccgcgagcgc
     4801 agccgcagca cctttcgggt cgcctgcctt aaatgcaagc gcgacatcgc ttgcgaacga
     4861 gggaaggagt gcaatgagac catctttatg ttcgcgcagg atctcgcggt ccatgcgcgg
     4921 tcgttcgttg aagccttcaa gaaacgagcg cgtcacgagc ttcataaggt tctcgtaccc
     4981 ttcgttattg cgcgcgagca gcacgatgcg cgcgcgcttt tcgttgtggt cgcggtcatg
     5041 acgcgatacc ggcgcaacat acgcgtccac accaaatacg ggcttcacgc cctgcttctc
     5101 gcactgcttg tagaactcaa cggcgccatg caggttcccc gcgtcggtca gtccgacagc
     5161 gtccatgccc tcggcctttg cggccttcac gagctcgggg accttcggaa gggcctgaag
     5221 gaaggagtag tgcgagtgcg cgtggaggtg gacgaatccc ggcatgggat aagaatacca
     5281 gacctgcctg tgtgcgtgtg atatcgtttc tgtatggcag aaaagcgcgg attcacgctt
     5341 attgagcttc tcgtggtgat cgcaatcatt ggcatcctct cgtcggtagt gctcgcatcc
     5401 cttaatacgg cgcgcggccg ggcgcgcgat tcgagtcgcc agtcagagct gcgccaaatg
     5461 cagactgcga tgcaggcgta ctacctgcag actggaaatt atccgggaaa tccaactccc
     5521 ggtagcgcga cctactctaa ctcgcctaat tggcagacca tatggcagcc gattatcgac
     5581 gcagggttca ttgggtcggc cccaacggga ccgaccggaa gtgattatcg ctatttcacc
     5641 tacggtgcag gcagtacgca gggcgcgatg ctggttacgg ttcttgaggc ggcgagcccc
     5701 tcgaacggat acccaggaac ctgccgtccg ttctcaggag tgaactggtg caggagcgac
     5761 atccagacga gggaatggtg tttgtgcaac ccgtactagg ctcatgatcg ttgtcggggt
     5821 cgatgaagca ggacgaggtc ctctagcagg acccgttgcc gtgggcgccg tagctgcaga
     5881 agaaggattc gatattcttg ccgcgtttcc aggactgaac gattcaaaac ggctttccga
     5941 gaagaagcgt gaggcgctgt ttgtgctttt gcagcaagca atccgcgatg gacatatacg
     6001 cgccacggtt acgctcatga gcgttaaaga gatagacggg gagggggtcg cgcacgcggt
     6061 gcggcacgga gtatggaaag gggtacgcaa gctccttccg aatcctgaag acgggaaagt
     6121 gtggctcgac ggctccttga aggcgccgcc tgaatacgaa caggacaccg tgattggagg
     6181 agacgggatc gttcctgcca tcatgctcgc gtctgtcgcg gcgaaggtaa cgagagaccg
     6241 ctacatgcag aaactcgatg ctacatatcc tttatacgga ttcgccgtgc ataagggata
     6301 tggcactaag gtgcatcgag aagcgattcg ggaacacggc ccatgcgaag cacatcggaa
     6361 gagcttcctt aggaacgtac tcaaatagga tagggcgcga cgattgctcg tcgcgccccg
     6421 gaacagcagt gatgctgttt gctcacgaga tctcggccta gaagccgtaa gacgaggtct
     6481 ggtgagaagc gctttgcctc gcgctcatga gggtgatcac cttcgccagc ttcgcaacgg
     6541 gggatggaga agaagatcga gccgtctctc cggcgggtcg gaggaagcct tgcggaacat
     6601 gaggatactc tctcgaggtt gctacaatgc agggccgttg gttggcctaa ggtcccgctt
     6661 aggacttact ggccctgctt cttctctata gcaccggctc cagaaaatac caaggaaaac
     6721 cgtacccctt ccgcaaataa ataccgtatg gtatggtgtc cccactatga ccgtacgaat
     6781 gcgccatacc cgttctcaca cccgcaaccg ccgcagccat cacgcgctgt cggagggcgc
     6841 tatcgtgaag gacaaggagt ccggcaacct ccgcctccca caccgcgtgg atgaagcaac
     6901 cggcatgtac aagggcatgc agatcgctca gcctaaggcg aagaaggcca agggtaagaa
     6961 agagggtcgc ggcgagcgct ctcagatcga ggtagtggac accacgcctg aagttgagaa
     7021 gaaggagcag aagaagacct ctatcctcag caagagcacg aagggtcgct ctacgacccg
     7081 ctctggcttt ggaggcggct caggctcgta ggtttctcca gaaaagatat gcacaggacc
     7141 tgcctctggc gggtcctgtg cttttgccgc tatactgtct tcacgctctt tccatatggc
     7201 caatagacat cttgcgcgct ctgtcgttct ccagactctc ttcgagtggg atacttctcg
     7261 attgtctgac ggggaagcaa ccgacgcgct ccagcgcaac gtcgaagaat tcggcggaga
     7321 cgataccgac cggccattca tggaaactct ccttgcaggc gtacttgcta agcgtgctga
     7381 tctcgatctc gttataacga aagctgcccc ggactggccg cttgagcgca tcgcgccagt
     7441 ggatcgcaat atcctccgta ttggtttgta cgagcttttg tttgcggatc gcacccaggt
     7501 gcctgcgaag gttgctatca acgaggcgat cgaactcgcg aaggtgttcg gcggggattc
     7561 ctcaggccgg ttcgtgaacg gcgtcctcgg cgctgtgtac aaggagcttg gcgagccggg
     7621 caaggacgaa aaaggaaaga aggaccgtgt ccgacgcgag gatcttccgg ttgaagagct
     7681 cggaggcgct atcgtctacg cgtatcacga gggtcagtac tatctcgctc tcgtgcatga
     7741 cgtgttcggg cactggacct tgtccaaagg aaagatccca gagggcatgt cggtagagga
     7801 agggaccgtg gatgggctca aggacgagct cggactccca gccgtcatta agacgaagat
     7861 cggcgaaaac gaatacgtgg cttcgcatcc tgagaagggg aagacccgaa agcacgtgtc
     7921 gtacttcctc gcggagtctc catacatgga gctcacgctc gagaagaagg gcgggcttga
     7981 tgacgcgcgc tggttccgtc tcacggacat cctcgagctc aatttctatg acgacatgct
     8041 tccgatcgtt actgaagcgg tgcagaagct cctcgacacg aagcgttgat tctgcgccat
     8101 attctggtat aatccgcctc aatgtctgat ttctcggcac atgcggcggc gctcggcgtc
     8161 tccttcaaga acctcgacct tctggttgag gcgttgacgc atcgttcgta cctgaacgag
     8221 catcgcaccg cagggaacca caacgagcgt ctcgagttcc ttggagacgc ggtcttggag
     8281 cttgcagtaa cgcgcttcct atttgagcgc ttcccaacga agcctgaagg cgacctcacg
     8341 gcataccgcg ctgcactcgt aaacacggta tcgctcgcgg agacgtccga ggcactcggc
     8401 gtgaatgaca tgctccttct ctcgaagggt gagcgcaagg atacgggtcg cgcacgagag
     8461 aacatcctcg ccaacgcgtt tgaggcgatc ctcggcgcta tctacctcga tcagggatac
     8521 gagagtgcgg aagcgttcgt agcaaatcat ctctacccaa agatcgatgc gatcctcgct
     8581 acacgcagct ggcaggacgg caagagtcat ttccaggagg tggcgcagga gaagcgcggc
     8641 atcacgccta cctacaagac tatccgtgag gaaggcccgg accatgacaa gcacttcacg
     8701 gttggcgtgt tcctcggcgc tgaggaggtt gcgcgcggag agggaaagag caagcaggaa
     8761 gcggagcagg cagccgcgca ggcagctctt gacattttct aaataaaaca atatagtttt
     8821 ccacagagcc tgcgcgcccc tgcgccggta cggagaacct cataatgaac cgtttcgcca
     8881 tgtattggct cctcatcacg atgggcctgg tgttcgcggc gacctatgcc gcttcggtgg
     8941 tctatccgct cggaaacgtc gtgatgtgga cggcggtggt cggcggcgcg ctcgccggaa
     9001 tcgcggcgtg cgtgtatggc cgcgagatca gcgaatcgcg tttcatcatc acgcttctgt
     9061 tcgcgctcct cgtcgtgttc ctgttgagcg acgcctacaa cccggggttc aatccggaag
     9121 cggccgtgaa gctcggcatc gccctcacgg tcgcaagtct gttcgcgctc ggctggacga
     9181 tcggcaacct gatccgcaag cccgagtgaa tctgcacgaa cgtgcacgcc gccccctgtc
     9241 tgcaaaggca cggggcggct tttccatacg ctatactgtt tttgaaatgc tgaagcgtct
     9301 tgagatagcg ggattcaagt cctttgcgaa gaagactacg ctcgacttct c
//
LOCUS       DASWXU010000033         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_23727, whole genome shotgun sequence.
ACCESSION   DASWXU010000033 DASWXU010000000
VERSION     DASWXU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23727"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..227)
                     /locus_tag="VGE48_01190"
     tRNA            complement(156..227)
                     /locus_tag="VGE48_01190"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Asn,seq:gtt)
     gene            316..555
                     /locus_tag="VGE48_01195"
     CDS             316..555
                     /locus_tag="VGE48_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1042020.1"
                     /translation="MILYMKSSCPFSAKVLIEASLLGIEFEERNVKDDKIAQELIEKG
                     GQLQTPYLIDEERGVAMYESDAIIDYLHATFTDGP"
     gene            596..961
                     /locus_tag="VGE48_01200"
     CDS             596..961
                     /locus_tag="VGE48_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY1042021.1"
                     /translation="MQIHFKGTNYELPSRVTALARRKVEALGKYLGRDREYARAYIDL
                     GKESEAHQNGKFWRTDINLDADGNRFYAKAVEESIEKAIDKAVAELASEIKTARKRQQ
                     SLVRKGGGVIKSLMRGFQG"
     gene            complement(939..1808)
                     /locus_tag="VGE48_01205"
     CDS             complement(939..1808)
                     /locus_tag="VGE48_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY1042022.1"
                     /translation="MTHKPFLFFGTPYVARDTLAFLLEKGYRPEAVVTSPDAPKGRGH
                     VLTPCDTKAFALEQGLEVLTPEHIDDAFIDFLSKYDAAYAIVVAYGKILPERLIGSFP
                     QGVLNIHYSLLPKYRGASPVEAALLNGDTETGVSIQRMVYELDAGDVLAAKTLSIQPE
                     ETTRELRPRLIALGSELLVEILPAFESGSLRGVPQDHAHATHTRKIKKESGELSLSGN
                     DTENWNKYRAYAESPGTYFFMEKDGARMRVKVRSAKLESGTFVPLRVVPEGKPETDYT
                     NLCSQGFIPESRA"
     gene            complement(1798..2316)
                     /gene="def"
                     /locus_tag="VGE48_01210"
     CDS             complement(1798..2316)
                     /gene="def"
                     /locus_tag="VGE48_01210"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY1042023.1"
                     /translation="MTPIVQDGEPVLRKIAEPVQDELFGTEELAKILKDMEESLDAQP
                     DGVALAAPQIGISLRIFIVRYDRIVAPEMPPLPADVGVYINPEFVKSSKRRVSMDEGC
                     LSVRGIYGKTERYDRATVRARTFDGATFERGGGGILAQIFQHETDHLNGILFIDHAED
                     LVEVRRDESHDA"
BASE COUNT          603 a          635 c          721 g          487 t
ORIGIN      
        1 ccggaacaca gttacgagag gaagctaagc gcaaagagtc agggaaagca gggcgcgtag
       61 cacagaaggg cggagcacct acatcgaaca aggctagagg agggaaaggt gcgagataaa
      121 agcaaaaaca ggccatgaag gcctgtttta ttttgctccg cgggcaggat tcgaacctgc
      181 gaccaatcga ttaacagtcg accgctctac cgctgagcta ccgcggaatg gtagccgccc
      241 tccgagggag ggctaaggca catagtagcg aaaatccccg attcggcaac agagggcttg
      301 gtatactaga gggtaatgat cctctatatg aaaagcagct gcccgtttag tgcaaaagta
      361 ctgatcgagg cctctctgct tggaatcgag ttcgaggagc gcaatgtaaa agatgacaag
      421 atcgcgcagg aactcataga gaaaggagga cagctccaga cgccgtacct tatagatgag
      481 gagcgaggcg tcgccatgta cgaatcggac gccatcattg actaccttca tgccaccttt
      541 actgacggtc cgtaaagtaa agggatacca ggttctctaa ttttccgaga ctcctatgca
      601 gatccacttc aagggcacga attatgagct tccgagccgc gtaaccgcgc tcgctcgcag
      661 gaaagtggaa gcactcggaa aatacctagg aagagatcgt gaatacgcga gggcatatat
      721 agacctcggg aaagagagcg aggcacacca gaacgggaag ttctggagga ctgacatcaa
      781 tcttgatgcg gatggtaacc gcttctacgc gaaagcggta gaagagagta tcgagaaggc
      841 gattgataag gcggtggccg agcttgcaag cgagatcaag accgcgcgca agcgccagca
      901 aagcctcgtt aggaagggcg gcggcgttat taagtcgctt atgcgcggct ttcagggata
      961 aagccctgcg aacaaaggtt cgtgtagtca gtctcgggct ttccttcagg taccactcga
     1021 agcggcacga acgtgccgct ttcgagtttc gccgagcgta ccttcacgcg catgcgcgca
     1081 ccatccttct ccatgaagaa ataggtgccc ggagattccg cataggcgcg gtacttgttc
     1141 cagttctcgg tatcgttgcc tgaaagagaa agctcaccgg actctttctt gatctttctc
     1201 gtatgcgtcg catgcgcatg gtcctggggg acgcccctaa gcgatcctga ttcaaaggca
     1261 ggaaggatct cgacgagaag ctcggaaccg agcgcgatga gacgggggcg cagttcacgc
     1321 gtggtctctt ccggctgtat ggaaagcgtc tttgctgcga gcacgtcgcc cgcatcaagc
     1381 tcgtacacca tcctctggat ggacacgccg gtctcggtgt caccgttcag aagtgcggcc
     1441 tctactggcg aggcgcctcg gtatttcgga aggagcgagt aatggatgtt gaggacacct
     1501 tgcgggaaag acccaataag cctttcggga aggattttcc cgtacgccac cacgatcgcg
     1561 tacgcggcat cgtactttga aaggaaatcg atgaacgcgt cgtcgatgtg ctctggcgtg
     1621 agcacctcga gtccttgttc aagagcaaac gctttcgtgt cgcaaggtgt gagcacgtgc
     1681 ccacgacctt taggagcgtc tgggctggtt acaacagctt cagggcggta tcccttctca
     1741 agaaggaacg cgagcgtgtc gcgtgcgacg tacggagtac cgaagaaaag gaatggctta
     1801 tgcgtcatgg gactcatcac gacgtacctc aaccaggtcc tcagcgtgat ctatgaaaag
     1861 gatgccgtta aggtgatcgg tttcgtgctg gaagatctgc gcaagaatac cgcccccgcc
     1921 gcgctcgaac gtagcgccgt cgaacgtgcg ggcgcgtacc gtagcgcgat catatcgctc
     1981 cgtctttcca tagatgccac ggaccgagag acacccctca tccatggaga cccggcgctt
     2041 cgagctcttt acgaattcag gattaatgta cacgcctacg tccgctggca acggcggcat
     2101 ctcaggagcc acaatgcggt cgtagcgaac aatgaagatg cgcagagaaa taccaatctg
     2161 cggagccgcg agcgcaacgc cgtctggctg cgcgtcgagc gactcttcca tatccttgag
     2221 tatctttgcg agctcctcgg ttccgaagag ttcgtcttgg accggttctg ctattttgcg
     2281 gagaaccggc tcaccgtctt ggacgattgg cgtcatataa ggactatata gcacaaaaac
     2341 gaaaaacgcc ggtcttgggg tgaaggcaag accggcgttt cgaaagatgc cgcgcaacga
     2401 ggcgccggac tgcggatacg catgccttcc aggggttggg tgacga
//
LOCUS       DASWXU010000034         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_994448, whole genome shotgun sequence.
ACCESSION   DASWXU010000034 DASWXU010000000
VERSION     DASWXU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994448"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="secY"
                     /locus_tag="VGE48_01215"
     CDS             complement(<1..1089)
                     /gene="secY"
                     /locus_tag="VGE48_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY1042024.1"
                     /translation="MFGKAIHKLKVVFSDRVIRNRILFILAAFAVFRFLAAIPIPGVD
                     QVALSQFFANNQFLGLLNIFSGGGLSSLSIVMLGVGPYITASIIMQLATVLIPKVKAM
                     YSEEGDAGRTKFTSIARALTIPLAVLQGVAFLSLLESQGIVRSLTPFDFAVNVFLVAA
                     GSMLLMWIGELITEFGIGNGVSLIIFAGIVAALPAGLSQVLFTATAANIPAYLGFALI
                     GLAIIFAVVLVSEAERSIPIHYARATRGAMSMAPTYLPIRLLQAGVMPIIFALSLLLL
                     PQMVLTGLSAIGVAGTAGALAAYNAFTLNPWAHGAVYFVLVVMFTYFYTSITFEPKRV
                     ADNLQKSGAFVPGVRPGRETETFIGNVVN"
     gene            complement(1094..1543)
                     /locus_tag="VGE48_01220"
     CDS             complement(1094..1543)
                     /locus_tag="VGE48_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HEY1042025.1"
                     /translation="MQSNTLAPKTERITSPRVGRGGKRGKTSGRGTKGQNARAGHRKR
                     PEMRDILKKLPKLRGHGQNRARTVRAKRREYAPVNLATLEAAFAAGDAVTPETLYKKG
                     LIPARGGRIAYVKILGTGSITKALKVSDCTASAAAKAAIEAAGGSLV"
     gene            complement(1555..2151)
                     /locus_tag="VGE48_01225"
     CDS             complement(1555..2151)
                     /locus_tag="VGE48_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY1042026.1"
                     /translation="MTDTTLDTNTEAKAADQQPAAPARDDRRGAPRGRGGMRGRGGDA
                     RGRGPRAPRERSEFEQATLEVRRVARVMAGGRRFSFSVTVVIGDKKGRVGVGLGKGAD
                     TALAIDKAVRDAKKHLVTVNRTADNSIAHDVTVKNSSSIVTIIPSPGRGIVAGSAMRQ
                     VLEHAGVTNVIAKILTRTKNKLTIARATVAALAQAGSR"
     gene            complement(2156..>2384)
                     /locus_tag="VGE48_01230"
     CDS             complement(2156..>2384)
                     /locus_tag="VGE48_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY1042027.1"
                     /translation="SAQLIDDVAGKTLVAAHGKTIGGAQSAQAAGVGKAIAEKAKAAG
                     ISSVVFDRGGYAYAGQVKGLADAARGAGLTF"
BASE COUNT          501 a          695 c          748 g          440 t
ORIGIN      
        1 attaaccacg ttcccgatga aggtctccgt ctcgcgaccc ggacgcacgc ccggaacgaa
       61 agcacccgac ttctggaggt tatccgctac gcgctttggc tcgaaggtaa tcgacgtgta
      121 gaagtacgtg aacatcacga cgagtacgaa atacaccgcg ccgtgtgccc atggattaag
      181 cgtgaaggcg ttgtacgcgg caagcgcgcc agccgtacct gcgaccccaa tcgcggagag
      241 accggtaagg accatctgcg ggagaaggag gagcgagagc gcgaagatga tcggcatcac
      301 gccagcctga aggagacgga tcgggagata tgtcggcgcc atagacatcg cgccgcgtgt
      361 tgcgcgtgcg taatggatcg ggatcgaacg ctcagcctca gacacgagca ccactgcgaa
      421 gatgatcgca aggccgatga gggcgaaccc gaggtatgcc gggatgttcg ccgcggttgc
      481 agtgaagagc acctgcgaga ggcctgccgg aagcgctgcc acgatacccg cgaagatgat
      541 gagcgagacg ccgttgccga tgccgaactc cgtgatgagc tcgccgatcc acataagaag
      601 catggaacca gcagccacga ggaatacgtt caccgcaaaa tcaaacggag taagcgaacg
      661 aacgatgccc tgactctcaa gcaaagagag gaacgctacg ccctgaagca ccgcaagcgg
      721 gatggtgagt gcgcgtgcga tagacgtgaa cttcgtgcgt cctgcgtccc cttcctcgct
      781 gtacatagcc ttcaccttcg gaatgagtac ggttgcgagc tgcatgatga tggatgcggt
      841 gatgtacggt cctacaccga gcatcacgat cgagaggctc gagagtccgc cgcccgagaa
      901 gatattcaaa aggccgagga actggttgtt cgcgaagaac tgcgaaagcg ctacctgatc
      961 gacacccgga atcggaatcg ccgcgaggaa gcggaatacc gcgaatgcgg caaggatgaa
     1021 gaggatccgg ttcctgatga cccgatccga gaagacgacc ttgagcttgt gaatcgcctt
     1081 tccgaacata cgattagacg agagagccgc cggcagcctc gatagctgcc ttcgctgccg
     1141 cagaggccgt gcagtcagaa accttgagcg ccttggtgat agaaccggtg ccgaggatct
     1201 tcacgtacgc gatacggccg ccacgtgctg ggatgagtcc cttcttgtag agggtctctg
     1261 gagttactgc gtcacccgca gcgaacgctg cctcgagggt cgcgaggttg actggagcgt
     1321 actcgcggcg cttagcgcgg acggtgcgag cgcggttctg accgtgaccg cgaagcttcg
     1381 ggagcttctt gaggatgtca cgcatctccg ggcgcttcct gtgaccggca cgagcgttct
     1441 gacccttggt accgcgacct gaggtcttgc cgcgcttacc gccacgacct acgcgaggtg
     1501 aggtgatgcg ctctgtcttc ggggcgagag tgttggactg catggtaaat aggattatcg
     1561 gctacctgcc tgtgcaagcg cggctacggt tgcgcgtgca atggtgagct tgttcttggt
     1621 gcgagtgaga atcttcgcaa tcacgttggt gacaccagcg tgctcgagta cctgacgcat
     1681 agcggaaccg gcgacgatac cgcgacccgg ggacgggatg atggtaacaa tcgaggaaga
     1741 gttcttcacg gtcacgtcgt gcgcaatgga gttgtccgcg gtgcggttaa ccgttacgag
     1801 gtgcttcttc gcatcgcgta ctgccttgtc gatagcaagt gcggtgtctg caccctttcc
     1861 aagaccaaca ccgacacgac ccttcttgtc gccaataact actgttacgg agaaggagaa
     1921 gcggcgaccg ccggccataa cgcgggccac gcggcgaacc tcaagggtag cctgctcgaa
     1981 ctcagagcgc tcgcgcggcg cacgaggacc gcgaccacgc gcgtcgccac cacggccacg
     2041 catgccgcca cgaccgcgag gagcaccacg acgatcgtcg cgcgctggag ctgctggctg
     2101 ctggtctgct gcctttgctt ccgtattcgt gtcgagagtg gtatcagtca tgtgtttaga
     2161 aagtaagccc tgccccgcgt gctgcgtcag cgagtccctt aacctgtcct gcgtacgcgt
     2221 agccgccgcg gtcgaacaca acgctcgaga tgcctgctgc cttcgccttc tcagcgatag
     2281 ccttgccgac acctgctgcc tgtgcgctct gcgcgccgcc gatggtcttg ccgtgcgctg
     2341 cgacgagggt cttccctgct acgtcgtcga taagctgtgc ggac
//
LOCUS       DASWXU010000035         5542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_26024, whole genome shotgun sequence.
ACCESSION   DASWXU010000035 DASWXU010000000
VERSION     DASWXU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26024"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VGE48_01235"
     CDS             <1..960
                     /locus_tag="VGE48_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY1042028.1"
                     /translation="LPPFGTGIIYGREYLNAADVLRKANPGDTISAKVVDAAGLDGYI
                     ELSLKEARQAAIWSEAEQAIAAQTIYTLTVDEANKGGLILSWQGIQGFLPASQLTKEH
                     YPRVPEGDKDKIFGELQKLTGTALSVRIITADAKEGKLIFSERVGDEENEKASLIDKY
                     QVGDVVEGEVTGAVDFGVFVKLEQGLEGLVHISELDWGLVEDPRALFKVGDKVSVKVI
                     EVKDGKVSLSVKALKENPWKTAQDRYKKGMEVSGVIIKYNKHGALASIEEGVAGLVHI
                     SEFASPAELRDTLELGRAYPFTITLFEPKDQRMTLSFAGAKKK"
     gene            complement(1003..1587)
                     /locus_tag="VGE48_01240"
     CDS             complement(1003..1587)
                     /locus_tag="VGE48_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042029.1"
                     /translation="MAYRILKVRSLKSLQKILKQHKVPFETWGQGPTKTLENFWKELQ
                     EGESVLAISGRGKLTRLVERSQALIYYEGPAGRLFLRESMQVFANGARRTRKQDISVS
                     EKRRCGESARSAMVRGISEELKIDLADPSVLERKSGHRYSSSKSSRSYPGLAAKNRMA
                     IFMWLMPIEHFDPEGYVERQRTKTTCFEWFPDKK"
     gene            complement(1640..2401)
                     /locus_tag="VGE48_01245"
     CDS             complement(1640..2401)
                     /locus_tag="VGE48_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1042030.1"
                     /translation="MQGIEIYRTRFANDIVAEYMPAARPSSHKVAILAAGCPGYPGGK
                     QEAMQLLARNGYWSIVPIYRGTWESRGEFLDKSPHEDLIDVMDGVENGFFDLWSGTEH
                     QMRDARFFLIGGSFGGAAALLASRDPRVEKTAVISGVVDWTRQDETLEPLKTMAEYIP
                     SAFGMGYRTHPDAWKKLARGDFYNPVAVKDELDPKKLLFIHSKDDLIVHAKPAEELAR
                     DIGARYVELQKHGHMGAGAIKKPVVWKHVARHFGK"
     gene            complement(2403..3002)
                     /gene="pth"
                     /locus_tag="VGE48_01250"
     CDS             complement(2403..3002)
                     /gene="pth"
                     /locus_tag="VGE48_01250"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY1042031.1"
                     /translation="MAHVIIGLGNPGGEYERTRHNAGRMVVELLAQEEGFPDFKLKKS
                     AEALVSEGSINGQKTVLALPEVYMNHSGKTAAGLVKSKPAAKKLLVVRDDLDLPIGTI
                     KMTTFGRGGGGHKGIESITRALKTKDFAQLKIGISAHTPKGKLKKVAAGEDVVKLVLG
                     KFKPGEDAELKRALTKAAEASRIFVSEGIERAMLYANTK"
     gene            3043..3357
                     /locus_tag="VGE48_01255"
     CDS             3043..3357
                     /locus_tag="VGE48_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEY1042032.1"
                     /translation="MKEYQEEVDAWFKEKGWEYWEPLAITARLYEEGGEFARLVNHVY
                     GPKKKKLDEHVQDMEEELGDIIYTLICFANSHGLSLDSAIRKSLDKVAGRDKDRFTEA
                     KS"
     gene            3388..3939
                     /gene="lepB"
                     /locus_tag="VGE48_01260"
     CDS             3388..3939
                     /gene="lepB"
                     /locus_tag="VGE48_01260"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY1042033.1"
                     /translation="MNVLGTAFREIFTFVVLAVVIVVPIRLFVAQPFVVEGESMHPTF
                     ESGDYLIVDQLSYHLGDPERGDVVVFRYPNDEKVFYIKRIIGLPGETVHVEGGETRVT
                     KADGTEITLDESYVVSEDVTYTLSRTLGPDQYFVMGDNRPKSSDSRTWGALPAHDIMG
                     RAYVRLLPFDEIGVLPGSVTVPE"
     gene            3944..5098
                     /locus_tag="VGE48_01265"
     CDS             3944..5098
                     /locus_tag="VGE48_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY1042034.1"
                     /translation="MPHTLQPSDIVKRAHAVGSYYGFTPLSTLAATKKGQGAKAPYPD
                     TLALDTLDTAAKDVAALLKQVRDAGLTPSAQQPLFFWHTNAAQGRPAPKQIVIQFHVL
                     GVEHAIADAVLIRAVRSLMHDLGKQEPVLRLNSMGDKETRGRLTRELTQLVRKHQHLI
                     PPEVAEHVRQGQPVRAAHLLAQDDNLKHFLPSPTDHLSEASRKHFEAVLEYLESTETP
                     YELSPHLYSRANAWTETCFELAHDADDSLHAWGSRYSELARPFFKGAVHSVGAVVRVN
                     APSRDTVAPIKERGNPRFVFVHIGDEAKRESMKMADELRKARIPLTQAMGVASLTEQM
                     RLAETMNPPYLLIMGRKEALERSVILRERATHTETFIPLNSLIERLKAVA"
     gene            5163..5333
                     /locus_tag="VGE48_01270"
     CDS             5163..5333
                     /locus_tag="VGE48_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042035.1"
                     /translation="MSTLKTIAGVICIGMGVYLGYTMAMTDSVFGGWAGTLAPTGLGA
                     IGALLIKSGFRR"
BASE COUNT         1125 a         1674 c         1547 g         1196 t
ORIGIN      
        1 ctcccgccgt tcggcaccgg tatcatctac ggccgcgagt acctcaacgc cgcagacgtg
       61 ctccgtaagg caaacccagg cgacaccatc tccgcgaagg ttgtcgacgc cgctggcctt
      121 gatggctaca tcgagctctc actcaaggag gctcgtcagg ccgctatctg gagcgaagct
      181 gagcaggcta tcgccgcaca gaccatctac acccttaccg tcgacgaggc gaacaagggc
      241 ggtcttatcc tttcgtggca gggcattcag ggcttcctcc ctgcttccca gcttacgaag
      301 gagcactacc cacgcgttcc tgagggtgac aaggacaaga tcttcggcga gcttcagaag
      361 ctcaccggca ccgcgctttc ggtccgcatc attaccgcgg acgcgaagga gggcaagctt
      421 atcttctctg agcgcgtagg cgacgaggag aacgagaagg catccctcat cgacaagtac
      481 caggttggcg acgtagtaga gggcgaggtt acgggcgctg ttgatttcgg cgtgttcgtg
      541 aagctcgagc agggtctcga gggcctcgtg cacatctcgg agctcgactg gggcctcgtg
      601 gaggatccgc gtgccctctt caaggttggc gacaaggtat ccgtgaaggt tatcgaggtg
      661 aaggacggca aggtatccct ttccgtgaag gccctcaagg aaaacccgtg gaagaccgcg
      721 caggaccgct acaagaaggg catggaggta tccggcgtta tcatcaagta caacaagcac
      781 ggcgcgctcg cttccatcga ggaaggtgtt gccggtctcg tacacatctc ggagttcgcg
      841 tctccagcag agctccgcga cacgctcgag cttggccgcg cgtacccatt taccattacc
      901 ctcttcgagc cgaaggacca gcgcatgacg ctctctttcg ccggcgcgaa gaagaagtaa
      961 cgctacaaac gaaggcaccc gggactcacg tcccgggtgt tttcatttct tgtcaggaaa
     1021 ccattcgaag catgtggtct ttgtcctctg gcgctcgaca tagccctcag gatcgaagtg
     1081 ttcgatcggc atgagccaca tgaagatcgc catgcggttc ttcgcagcaa gccccggata
     1141 cgaccgggac gacttcgaag aagagtaacg gtgcccggac ttgcgctcga gcacgctcgg
     1201 atcagcgagg tcgattttca gttcctcgct gatgccacgg accatggcgg accgagccga
     1261 ttccccacac ctgcgcttct cgcttacgct aatgtcttgc tttcgcgtac gacgtgcgcc
     1321 gttagcgaac acctgcatgg attctctcag gaacaggcgt cccgccggtc cttcgtagta
     1381 gatgagcgct tgcgagcgtt cgacgagtcg cgtcagcttg cctcgtcctg aaatggcgag
     1441 cactgattct ccctcttgaa gctccttcca gaagttctcg agggtcttcg tgggaccttg
     1501 gccccaggtc tcgaaaggaa ccttatgctg cttcaaaatc ttttgaagcg acttgaggct
     1561 ccggactttt agtatgcgat atgccatctg gcacctccct ccgcttccgg agggaatgta
     1621 gcacgctttc ttctaaacgc tacttcccga agtgccgtgc cacgtgcttc cagacaacag
     1681 gcttcttgat ggcgcctgcg cccatgtgcc cgtgcttctg cagctcgacg tagcgagcgc
     1741 ctatgtctct tgcaagctcc tcggcaggct ttgcatgcac gataagatcg tccttggaat
     1801 gtatgaagag gagtttcttc ggatcgagct catccttcac cgcgaccgga ttataaaaat
     1861 ctcccctcgc gagcttcttc caggcgtccg ggtgcgtgcg gtatcccata ccaaatgcgc
     1921 tcgggatgta ctcggccatg gtctttaagg gctcaagcgt ctcgtcctga cgcgtccagt
     1981 ctacgacgcc cgagatgacg gcagtcttct ccacacgggg atcgcgcgac gcaagaagcg
     2041 cggcagcgcc gccaaagctt ccgcctatga gaaagaaccg tgcatcgcgc atctggtgct
     2101 ccgtaccact ccagaggtcg aagaatccgt tctctacgcc gtccatgaca tcgataagat
     2161 cctcgtgcgg actcttgtcg aggaactcgc cccgactctc ccaggtgccg cggtatatgg
     2221 gcacaatgct ccagtagccg ttccgcgcaa ggagctgcat cgcttcttgc ttgccacccg
     2281 ggtatcccgg acaacccgcc gcaagtatcg caaccttgtg cgaagacggg cgcgctgccg
     2341 gcatatattc ggcaacgatg tcgtttgcaa aacgcgtacg atatatctcg attccctgca
     2401 tgctatttcg tgttcgcgta gagcatcgcg cgttcgatac cttctgagac aaaaatgcgc
     2461 gacgcttctg cggccttcgt gagagcacgc tttagttccg cgtcctctcc cggcttgaac
     2521 ttcccgagga cgagcttcac gacgtcctct cctgccgcta ccttcttcag cttccccttc
     2581 ggagtatgcg ccgagatgcc gatcttaagc tgcgcgaagt ccttggtctt gagcgcgcgg
     2641 gtgatgctct cgatgccctt atgtccgcct ccgccgcgcc cgaaagttgt catctttatg
     2701 gtgccgatcg gaagatccag atcatcccgc actacgagca gtttcttcgc tgctggcttg
     2761 ctcttcacga gtcccgctgc ggtctttcct gagtggttca tgtatacctc aggaagcgct
     2821 aggacggtct tctgtccgtt gatagatcct tccgatacga gtgcttcggc gctcttcttc
     2881 agtttgaagt ccgggaagcc ttcttcctgc gcgaggagtt ccaccaccat gcgtccggca
     2941 ttgtgccggg tgcgctcgta ttcgccgccc gggttcccga ggccgatgat gacgtgtgcc
     3001 atggggctat cctagcattt tcgtggtggt accatggttt ctatgaagga ataccaggag
     3061 gaagtcgacg catggttcaa ggagaagggc tgggaatact gggaaccgct tgccataacc
     3121 gcgcgtcttt atgaagaagg cggcgagttc gcgcgtctcg tgaatcacgt atacggcccg
     3181 aagaagaaaa agctcgacga gcacgtacag gacatggaag aggagctcgg cgatatcatc
     3241 tacaccttga tctgctttgc aaactcacac ggactctcgc ttgattccgc tatcaggaag
     3301 agcctcgaca aggttgcggg gcgtgataaa gatcgcttca cagaagcaaa aagttgacgc
     3361 gcagattcag aggaatacaa tgcgcgcatg aacgttcttg ggactgcgtt tcgtgagata
     3421 ttcaccttcg ttgtccttgc ggttgtcatt gtcgtcccaa tccgcctctt cgtggcgcag
     3481 ccgttcgtgg tcgaaggcga aagcatgcac ccgaccttcg agtccggcga ttacttaatt
     3541 gtcgatcaac tctcctatca tctgggcgac cctgagcgcg gcgacgtggt ggtgttccgc
     3601 tacccaaacg acgagaaagt cttctacatt aagcgcatca ttggtcttcc gggcgaaacg
     3661 gtgcatgtcg agggcggtga gacccgcgtt acgaaagccg acggcactga gataacgctt
     3721 gatgagtcct atgtcgttag tgaggacgta acctataccc tctcccgcac gctcggcccg
     3781 gatcagtact tcgttatggg cgacaaccgc ccgaagagct cggactcacg cacctgggga
     3841 gcattgccgg cgcacgacat tatgggccgt gcgtacgtcc gcctgcttcc gttcgatgag
     3901 atcggggtgc ttccgggttc agtaaccgtg cctgagtaga cctatgccac acacgcttca
     3961 gccttcagac atcgttaagc gggcgcatgc agtcggaagc tattacggct ttaccccgct
     4021 ctcaacctta gcggcaacta agaagggcca gggcgcgaaa gctccgtacc cggacacgct
     4081 cgcactcgat acgctcgata cggcagcaaa ggatgttgct gcacttttga aacaagtgcg
     4141 cgatgccggc cttacgccgt ctgcgcagca gcccttgttc ttctggcaca cgaatgctgc
     4201 gcagggtcgc cctgcgccaa agcagatcgt catccaattt cacgtgctcg gcgttgagca
     4261 tgcgatcgcg gacgcagtac tcatccgcgc cgtgcgctcc ttgatgcatg atcttgggaa
     4321 gcaggaaccg gtacttcgcc tcaacagcat gggagacaag gagacccgcg gacgccttac
     4381 ccgcgaattg acccaactcg tgcgtaagca ccagcacctc attccgccgg aagtagcgga
     4441 gcatgtacgt cagggacagc cggtccgcgc tgcacacctc cttgcgcagg acgataacct
     4501 gaaacacttc cttccgtctc caaccgatca cctttccgaa gcaagccgca agcacttcga
     4561 ggcggtgctc gagtaccttg agtctacgga aaccccgtac gagctctcgc cgcacctcta
     4621 ctcacgcgca aacgcgtgga ccgagacctg cttcgagctg gcgcatgacg cggacgatag
     4681 cctccatgcg tggggatctc ggtacagcga gctcgcgcgt ccgtttttca aaggagcggt
     4741 acattctgtt ggcgccgtgg tgcgtgtaaa cgctccgagc cgcgatacgg tcgcgcctat
     4801 caaggagcgc ggaaatcctc gcttcgtgtt cgtgcacatc ggcgacgaag ctaagcgcga
     4861 gagcatgaag atggcggacg agctccgcaa agcccgcatc ccgctcacgc aggccatggg
     4921 cgtcgcgtcc ctaacggagc aaatgcgtct cgcggagacc atgaaccctc cgtatctcct
     4981 catcatgggc cgcaaggagg cgcttgagcg ctccgttatc ctccgagaac gagccaccca
     5041 cacagagaca ttcattccgc ttaactctct tatagagcgg ctgaaggcag ttgcgtagta
     5101 cttgacaaat attttaataa gtgtattatg gtaaaggatc aacgctcaag agggagagac
     5161 caatgagcac cctgaaaacc atcgccggag tcatctgcat cggcatgggt gtctacctgg
     5221 gctacaccat ggccatgacg gattcggtgt tcggcggctg ggccggcacc ctggcgccca
     5281 ccggcctcgg agcgatcggc gccctgctga tcaagtccgg cttcaggcgg taaatcagcc
     5341 ccgtcacgac accagcgagc gcccgcagtc ttcggactcg cgggcgcttt ctcatatcca
     5401 ggaattgcgc ccttttaggc cctatggtat cttttccttc atgcaaaacg cagtacgcgt
     5461 agaggtaagc cgtggcaaga acgagagctc ggcatctctt attcgccgct tttcccgccg
     5521 tggccagggc ctcggtctcg tt
//
LOCUS       DASWXU010000036         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_487541, whole genome shotgun sequence.
ACCESSION   DASWXU010000036 DASWXU010000000
VERSION     DASWXU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487541"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..271)
                     /locus_tag="VGE48_01275"
     tRNA            complement(198..271)
                     /locus_tag="VGE48_01275"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Val,seq:gac)
     gene            complement(370..654)
                     /locus_tag="VGE48_01280"
     CDS             complement(370..654)
                     /locus_tag="VGE48_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042036.1"
                     /translation="MLLMAAAALCVSGCATTGSMNPFTMLSTADDETMYRHAASTIGV
                     SRDQMTISNQTRVGDRTSFTVTTAAGAEYRCTVSVAFTTVNDITCPPSGN"
     gene            complement(754..2076)
                     /locus_tag="VGE48_01285"
     CDS             complement(754..2076)
                     /locus_tag="VGE48_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1042037.1"
                     /translation="MKAFYNILGNTLVASITNFTVWFAITFWIFLETESVFATGMLAG
                     IYLVLTAGTGFWFGSIVDHNRKKNVMLASSVISFVLYGLAFLLFQSVPESVFADVSSV
                     TLWAFVLLMMIGVCAGNLRTIALPTLVTLLIPEDRRDKANGLVGMTTGIGFLITSMIS
                     GFLVAWGGMFYVIVFALSLTALAFLHLSFVAVDETRASGDPEAPKEAKRIDIKGTICV
                     IRGVPGLMALILFATFNNFLGGIFMALMDAYGLSLVSVQTWGVLIGVLSTAFILSGII
                     IAKTGLGKSPLRTLMIVNIIAWTTTSLFTIQSSIVLLAIGFFVWMFISPYAEAAEHTT
                     LQKVVPFERQGRVFGFAQSVEQAASPLTAFLIGPLTQFFFIPLMTTGLGAATIGSWFG
                     TGPERGIALVFTVAGALGLLVTILAFNSKYYRQLSARYLEGAPSKALT"
     gene            complement(2116..2190)
                     /locus_tag="VGE48_01290"
     tRNA            complement(2116..2190)
                     /locus_tag="VGE48_01290"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2155..2157),aa:Glu,seq:ttc)
     gene            2263..2352
                     /locus_tag="VGE48_01295"
     tRNA            2263..2352
                     /locus_tag="VGE48_01295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2297..2299,aa:Ser,seq:cga)
     gene            2360..>2736
                     /locus_tag="VGE48_01300"
     CDS             2360..>2736
                     /locus_tag="VGE48_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042038.1"
                     /translation="MALSLPTPKPCKDCGDGAIFHRITYVSIVLDDLMKPLTRPSMFL
                     RGFASIVYAIERRVTPHLLEGMMNLGWATRVTEPDDDTQLLALMLWNEAKDRGMSVSE
                     FRLFNLPRNIFVAAYPDGRKIAY"
BASE COUNT          660 a          728 c          802 g          546 t
ORIGIN      
        1 gggttgcgaa aacagacagg gcagccagga gaaatagtgc gcccataagc atgaacacac
       61 tcgaagtttt ctcgaagaat gcgtcaaacc cgttttgaaa cgactcaatc atgatgagcc
      121 tccttccatg atccatgcta gcaccaagct aaatacgaaa tggctcccgt acacaggagc
      181 cacttcttct gtctcggtgc gcggtgagga attcgaatcc ccgaccttcc ccacgtcaag
      241 gggacgctct aaccagctga gctaaccgcg cgcgtgttcg cacgataccg catccggaga
      301 taccattcaa gcataagaaa aggccccggc gtagagcgcg gggccttcgt cttgcgttgt
      361 cgattcgggt cagttgcccg agggcggaca ggtgatgtcg ttgaccgtgg tgaaggccac
      421 ggacacggtg cagcggtact cggcgccggc ggccgtcgtc acggtgaagc tcgtccggtc
      481 gccgacacgg gtctggttcg agatggtcat ctggtccctg ctgacgccga tggtgctcgc
      541 cgcgtggcgg tacatggtct cgtcgtcggc tgtggacagc atggtgaagg ggttcatcga
      601 acccgtggtg gcgcagccgg agacgcagag cgccgcggcc gccatgagca gcatacgcat
      661 gggcttttcc ctcaaaaagc gcgccaggat ggcgggccta ccgtgccgaa tcgaagaacc
      721 ggcatctgtt cgcaccgtac ctcaaattaa gcattaggtc aatgcttttg aaggagcgcc
      781 ttcaaggtag cgtgctgaga gctgacggta gtacttcgag ttgaaggcga ggatggttac
      841 caaaagacca agcgcaccag caaccgtaaa cacgagcgca atgcctcgtt caggcccggt
      901 gccaaaccaa ctgccgatag ttgcggcacc aagcccggtt gtcatgagtg ggatgaagaa
      961 gaactgggtc aggggaccga tcaggaatgc cgtgagcggg gatgcagcct gttcgacgct
     1021 ttgcgcgaat ccgaatacgc gaccctgacg ctcaaaaggc accaccttct ggagcgtggt
     1081 atgctcggcc gcttctgcgt atgggctgat gaacatccaa acaaagaagc cgattgcgag
     1141 caagacaata gaggactgga tagtgaagag gctggtagtg gtccacgcga tgatgttcac
     1201 aatcatgaga gtgcgcagcg ggctctttcc gagtccggtc ttcgcgatga tgatgccgga
     1261 gagtatgaat gccgttgaga ggacaccgat gagtacaccc caggtctgga ccgagacgag
     1321 cgataggccg tacgcatcca tgagtgccat gaagatgcca ccgaggaagt tattgaaggt
     1381 ggcaaaaagg atgagtgcca taaggccggg aacgccgcga attacacaga ttgtgccctt
     1441 gatatcgata cgcttcgctt ctttcggtgc ctctgggtct ccgcttgcac gggtctcgtc
     1501 taccgcaacg aacgagaggt gtaggaacgc aagcgcggtg agtgagagcg cgaacacgat
     1561 cacataaaac atgccgcccc aggcgacgag gaatccacta atcatagatg tgatgaggaa
     1621 tccgatacca gtcgtcatac ctacgagtcc gttcgccttg tcgcgccggt cctcgggaat
     1681 aaggagggta acgagcgtcg ggagtgcgat agtgcggagg tttccagcgc acacaccgat
     1741 catcatgaga agcacgaacg cccagagcgt tacgcttgag acatcagcga acacagactc
     1801 gggcacggac tgaaacagga gaaaggcaag tccgtagaga acaaacgaga tcacgctcga
     1861 cgcgagcatc acgttcttct tgcggttatg gtccacgatg cttccgaacc agaagccggt
     1921 tcctgcggta agtacgaggt aaatacctgc gagcatgccg gtagcaaata cggattctgt
     1981 ctcaaggaag atccagaagg tgattgcgaa ccagaccgta aagttggtta tcgacgcgac
     2041 aagtgtgttc ccaaggatgt tatagaaagc tttcatagcg gtatcggacg atactaacat
     2101 cctttcccga acttttggtg accctacggg gaatcgaacc ccgatttacg ccgtgaaagg
     2161 gcgctgtcct agccgttaga cgatagggcc gtctgtatac gcatcctaca tttttgaggc
     2221 cagaaatgct aggatgcgtg agttctcgac ttcaaacctt taggagaggt ggctgagtgg
     2281 tcgaaggcac cgcactcgaa atgcggcgta cgggaaactg taccgggagt tcgaatctcc
     2341 ccctctccgc caaatacgta tggctctttc gcttccaact cctaagcctt gtaaagactg
     2401 cggcgacggc gctattttcc atcgcatcac gtacgtgagc atcgtgttag acgatctcat
     2461 gaagccctta acgcgcccaa gcatgttcct gcgcggattc gctagcatcg tgtacgcgat
     2521 tgagcgcagg gtaacgccgc atcttctcga aggaatgatg aacctcgggt gggcgacgcg
     2581 cgtaacagag cccgacgacg atacccaact acttgcgctt atgctctgga acgaggcgaa
     2641 ggatcgcggt atgtctgtat ccgagttccg cctctttaac cttccgcgca acattttcgt
     2701 agccgcatac ccagatggca ggaagatcgc gtacga
//
LOCUS       DASWXU010000037         4134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_649903, whole genome shotgun sequence.
ACCESSION   DASWXU010000037 DASWXU010000000
VERSION     DASWXU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649903"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VGE48_01305"
     CDS             <1..394
                     /locus_tag="VGE48_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042039.1"
                     /translation="AIIGILSSVVLASLNTARNRGADAAIKSNLANARAEAELYYDGN
                     SNSYGGGTSVTANAGVCAASGTNVIGDNIAAATSAGSGSVQCQASNTLWAAFAQMKSS
                     TSQYYCVDSSGYASTTSSAPTISDGACP"
     gene            494..967
                     /locus_tag="VGE48_01310"
     CDS             494..967
                     /locus_tag="VGE48_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1042040.1"
                     /translation="MNYHSVARGFTLIELLVVIAIIGILSSVVLASLNTARNRGADSS
                     IKSNLANSRSEAELFYDTNNMSYEGVCATTGANRIGDSVNAAENAYDGSPTTYADGTA
                     STWQTGQCHDSTNAWAAWVPLKASASGSIVAFCVDSTGVAKQVTAVLGASSYACP"
     gene            970..1731
                     /locus_tag="VGE48_01315"
     CDS             970..1731
                     /locus_tag="VGE48_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY1042041.1"
                     /translation="MHVIGVIGFALLGGVLASFLGVVAERVYTGQSWIRDRSRCNSCS
                     EPLGWRDLLPVLSFVTTFGRARCCGARLPWTYVASEFLLGVVFALAYLKLGLGVPLLV
                     FLLAILTLAFIILYDLRHTVVPLAASSVLMVLSLGFAWLSSRAIPEFGLTLSVAGFVG
                     LFFFLFYAFSRGRAMGLGDAPVALSLALLVGPYLALPGLLFSFWIGAAVGIAILVGRP
                     KGHRMGIEVPFVPFLAAGFLLAYFTQWNPLLLQLS"
     gene            1701..2423
                     /locus_tag="VGE48_01320"
     CDS             1701..2423
                     /locus_tag="VGE48_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY1042042.1"
                     /translation="MESAPSSAFITSRGFTLVEMVVVLAIIVIITTIALLGQSSFNRS
                     LVLTDISYTIAFSIREAQSYGISSRRSGSTTNAGYGVHFSAGSPNAYTLFADVDPVRP
                     GDVSNPSVCPGHPNLSSANPESKPGNCIQDTATETVRSYSLNNGFRIKSFCGTRASNG
                     QQVCSDSGIVALDILYMRPNTQSVITGITSSAVPPPLSSRIALTDATIRVVSPDGVGE
                     RCVLVTKVGHVSVAQKGDEGCP"
     gene            2420..3004
                     /locus_tag="VGE48_01325"
     CDS             2420..3004
                     /locus_tag="VGE48_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1042043.1"
                     /translation="MTNNTSQRGYSLLELIVSLGIFSVVMLVVTGAYLTLISLDRQAR
                     ANNQLAATLSFATESMARSMRTGSEYACNGNTSSPNCSGGGTSISFCLEGQDCRSGGG
                     TQYLVSYRLKSDGSIGQCTGSVCTDATAVSLTDPRITVNALRFYVRGVGTSGADAYLQ
                     PQVIFVVSGTMVTDSGESTSFSIQTAATQRLIEI"
     gene            3013..3543
                     /locus_tag="VGE48_01330"
     CDS             3013..3543
                     /locus_tag="VGE48_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY1042044.1"
                     /translation="MEQRGFTLVETLVAITIIAVAIIGPLYAVQQALNASRVARDVLI
                     ASSLAQEGVEYVRAVRDGNYITNLISPGSRNWLSGFDGTAGGTATYANCITADCVVDP
                     TQNTISRTITALYLSSAGLYNQQASGQATKFTRRVRLTAVPGSTTEMVVTVTVSWSVL
                     GQTQTVTVTDRLHNWL"
     gene            3546..4007
                     /locus_tag="VGE48_01335"
     CDS             3546..4007
                     /locus_tag="VGE48_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042045.1"
                     /translation="MHPSRGFTLLIAVILTSVLLSVGLALLDISYKQIVLSSTARQSQ
                     YAFYAADSAIECALYWDQKENAFAYATPIGTIQCAGSNISVSSSVSGGTRTSTFATPC
                     VDGVTASVTVYKTTGASCSSGSATSCIYATGYNTCTATDPRRIERGLKVFY"
BASE COUNT          821 a         1166 c         1164 g          983 t
ORIGIN      
        1 cgcgatcatc ggcatcctct cgtctgtggt gctcgcatcg ctcaataccg cacgtaaccg
       61 cggtgcagat gctgccatca agtcgaatct tgccaacgca cgcgcagagg cagagcttta
      121 ctacgacggc aacagcaact cgtacggtgg tggtacctcc gtcactgcga acgcaggcgt
      181 ttgtgctgct tctggcacca acgttatcgg tgacaacatc gccgcagcca cctctgcagg
      241 ctctggttcg gtacagtgtc aggcaagtaa cacgctctgg gcagcatttg cgcagatgaa
      301 gagcagcacc tcgcagtact actgtgtgga ctcctctggc tacgcaagta ccaccagctc
      361 ggctcctacc atctcggacg gtgcctgtcc ataatttccg aatctctcgg atagcaaaag
      421 cgcccagggg gcgcttttgt gtttaataca cagagcgcca ggttgtacgg aaggaagcgt
      481 gtatagtgga gatatgaact atcattccgt tgcaagaggt ttcaccctca tcgagcttct
      541 cgtggtgatc gcgatcatcg gcatcctctc gtctgtggtg ctcgcatcgc tcaacaccgc
      601 acgtaaccgc ggtgcagact cttctattaa gtcgaatctt gcgaactcac gctctgaggc
      661 cgagctcttc tacgacacca acaatatgag ctatgaaggc gtatgcgcga cgacgggcgc
      721 aaatcgtatc ggggacagcg tgaatgctgc ggagaatgcg tatgacggat caccaactac
      781 ttacgctgat ggtacggctt caacctggca gacgggtcag tgccacgact ccacgaatgc
      841 ctgggctgcg tgggttccac tcaaggcatc agcaagcggt tcgattgttg cgttctgtgt
      901 agacagcact ggcgtagcta agcaggtgac tgcagtactt ggagcctcaa gctacgcgtg
      961 cccatagaga tgcacgtcat cggggtcatt gggttcgcgc ttcttggggg cgttcttgca
     1021 agcttcctcg gggtagtagc cgaacgtgta tacacgggac aatcgtggat ccgggatcgg
     1081 tcaaggtgca attcctgcag tgaacctctc ggttggcggg acctgctgcc ggtgctttca
     1141 ttcgtcacaa cctttggcag ggcccgttgc tgcggtgcac ggctcccgtg gacctatgtg
     1201 gcgagcgagt tcctgctcgg tgttgtgttc gcgcttgcgt atctcaaact tggtttgggt
     1261 gttccgcttc tcgtgttcct gctcgcgatc cttacgctcg catttattat cctctatgac
     1321 ctccgtcata ccgtggttcc tcttgcggcg tcttcggttc ttatggttct ctcgcttgga
     1381 tttgcatggc tctcgtcgcg cgcgattcct gagttcggac tgacgctttc agttgccgga
     1441 ttcgtcggac tgttcttctt ccttttctat gccttctctc gggggagggc tatgggtctc
     1501 ggggacgctc cggtggcatt atcgctcgcg cttctcgttg ggccgtatct cgcgcttcct
     1561 ggactgctat tctcgttttg gataggtgca gccgtgggca tagcgatatt ggttggccgc
     1621 cccaaaggcc atagaatggg tattgaggtc ccgttcgtac cgttcctcgc cgcaggcttc
     1681 ctgctcgcgt acttcaccca atggaatccg ctccttcttc agctttcata acgtcgcgag
     1741 gcttcaccct cgtggagatg gtcgtggttc ttgcgattat cgttatcatc acgacgattg
     1801 cgctactcgg ccagagctcg ttcaaccgct cactcgtact tacagacatt tcctacacca
     1861 ttgcgttttc tatacgtgaa gcgcagtcct acggtatctc gagtaggcgc tcggggagca
     1921 ctacgaatgc cggatacggc gtacatttct ccgcaggatc accgaacgcg tatacgcttt
     1981 ttgctgacgt ggaccctgtg cgtcctggcg acgtaagcaa cccttcggtg tgtccaggac
     2041 atccgaatct ttcaagtgcc aacccagagt caaagcccgg caactgtatt caggatacgg
     2101 cgaccgaaac ggttcgaagc tactcgctta ataacgggtt ccgtatcaag agtttctgcg
     2161 gcactcgggc aagcaacgga cagcaggtct gcagcgactc gggcatcgtt gcgctcgata
     2221 tcctctacat gagaccgaac acccagtctg ttatcaccgg cattacttca agtgcggtcc
     2281 cgccaccgct ttcttcgcgc atcgcactta cggacgcaac gatacgtgtg gtatcgccag
     2341 acggagtggg ggagcgctgt gtgctcgtaa cgaaagtagg gcacgtgtcc gttgcacaga
     2401 agggagacga aggatgccca tgacaaacaa cacttctcag cgcggatact cgcttctcga
     2461 gctcatcgta tcgctcggca tcttcagtgt ggtgatgcta gtggttaccg gtgcgtacct
     2521 cacgctcata agtctcgacc gccaggcgcg tgcgaacaac cagcttgcag cgacactgtc
     2581 gtttgcgacg gagagcatgg ctcgctctat gcgtaccggt agcgagtacg cctgcaacgg
     2641 aaatacttcg tcaccaaatt gctctggcgg aggtacgagc atttcattct gtcttgaggg
     2701 acaggactgc agaagtgggg gaggaacgca gtatctcgtt tcctatcgcc tgaagagtga
     2761 cggcagtatc gggcagtgca ccggaagtgt ttgtaccgac gcgaccgccg tgtcgctgac
     2821 agatccgcgc atcacagtga atgcgcttcg cttctacgtg cgcggggtgg gtacctcagg
     2881 agcggacgcg tatctgcagc cgcaggtcat cttcgtagtg tcaggtacca tggtgaccga
     2941 ttccggcgag agtacgagct tctctatcca gactgcagca acgcagcgac ttatcgaaat
     3001 atgatgcacg taatggaaca gcgcgggttc acgcttgtcg agacgctggt tgcgatcacg
     3061 attatcgcgg tcgcaatcat cggtcccttg tatgcggtac aacaggccct gaacgcgtcc
     3121 agggtagcgc gtgacgtgct tatcgcgtcc tctcttgcgc aggaaggagt tgaatatgta
     3181 cgagcggttc gtgatggtaa ttacataacg aatctcatct ctcccggaag tcgcaactgg
     3241 ctttctggat tcgatgggac tgcaggtggg acggctacgt atgcaaactg cattaccgcc
     3301 gactgcgtgg tggatccgac acaaaacacc atttcgagga cgatcacggc gctctacctc
     3361 agttctgccg ggctctacaa ccagcaagca agcggacagg ctacgaagtt cacgcgcagg
     3421 gtacgtctca cggcggtccc tggcagcact accgagatgg tagtgacggt gacggttagc
     3481 tggagcgtgc tcgggcagac acagacggtt accgtgacgg accgtctcca taactggcta
     3541 tgatcatgca tccttctcgc gggttcacac tccttatcgc ggtcatcctg acttcggttc
     3601 tcctttccgt gggtcttgca ctccttgata tctcatacaa gcaaatcgtg ctctcctcga
     3661 cggcacgtca gtcgcagtac gcgttctatg ccgcggactc agctattgag tgcgcgctct
     3721 attgggacca gaaggagaac gcgttcgcgt acgctacccc gataggcact atccaatgtg
     3781 caggctccaa tatttcggtt tcctcgagtg taagcggcgg taccaggact tccacgttcg
     3841 cgactccgtg cgtggacgga gtgaccgcct cggtcaccgt ctataagacc acaggcgctt
     3901 cctgtagctc cggctccgca accagctgta tctatgctac gggatacaac acctgtaccg
     3961 cgacagatcc gcgccgaatc gagcgaggtc ttaaggtctt ctactagcgc tcgggtaggg
     4021 tagagtaagg gtaatggcgc cccggatacc tgaagacgcg aaggaacgag ccgcgaagct
     4081 gcgcgcggct atttctggat atcgggccga atatcacgag aaggacgagt cttc
//
LOCUS       DASWXU010000038         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_195774, whole genome shotgun sequence.
ACCESSION   DASWXU010000038 DASWXU010000000
VERSION     DASWXU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195774"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGE48_01340"
     CDS             <1..484
                     /locus_tag="VGE48_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042046.1"
                     /translation="GLLMTAFVHQAEAREAHKRADGTTLEVSINNAGGVLVRGAEVTA
                     VSGSVITARTEWGASALTWTVDTNGDTDFVSRGNQNLDIDDIDIGDTVSFSGALDVDG
                     SAFNVDADVVKNWSLDTNDTREARDEARVEAKTELKAKWGDWMRGIPLMNWFNKHDNR
                     "
     gene            539..2212
                     /gene="argS"
                     /locus_tag="VGE48_01345"
     CDS             539..2212
                     /gene="argS"
                     /locus_tag="VGE48_01345"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY1042047.1"
                     /translation="MEETLSLAIRTALDEMGAENALFTIEWPADLAHGDFATNAALAA
                     AKPLGRSPREIADELAGKLRDVLGEQVASIEVAGPGFVNVTLARDAIAGAIRSVPAEG
                     WGSGSARSEERIAFEYSCPNPFKEMHVGHLMSTVIGEAASRLIENQGARVLRETYGGD
                     VGPHVAKALWALRRKGNDEPATPEEIGEAYAQGSRAYEESEEAKAEIDALNVSLYQAL
                     AKEEGERSDEDRTLLELWRHGRDASLAAHKAVWDALGTHFDYVFHESETTPIGMEVVT
                     RAREEGVFVESQGAVIYDGEKKGLHTLVFLTSRGTPTYEAKEMGLAFLKEERIGPLDH
                     AYITTAAEQKGHFEVFLAALTDVAPELAQKIEHVPHGFLRLTTGKMSSREGNVITGAG
                     LISEIIDTAREKNQDPFVAKAVAIAAIKYMILRQAAGGDIIFDPEKSLSLDGDSGPYL
                     QYALVRARSVLAQAKKPENIKAPLEPYALERLIIRFPEVARRAEAELAPHKVAQYLTQ
                     LAGEWNSFYANNRIIGGEHEGYKLLLARAFALTMEQGLNLLGIPVPERM"
     gene            2331..2534
                     /locus_tag="VGE48_01350"
     CDS             2331..2534
                     /locus_tag="VGE48_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042048.1"
                     /translation="MDVLAQLARERRMKIDEIFWACAAWLRARGERVKNFTIRNWLSR
                     YKQTRRIHPKVAHFAQHLQAASA"
BASE COUNT          578 a          687 c          820 g          488 t
ORIGIN      
        1 aggacttctc atgactgcct ttgtacatca ggcagaagca cgtgaggcac acaagcgggc
       61 agacggaacg acgcttgagg tctctattaa taacgcaggc ggcgtgctcg ttcgcggcgc
      121 cgaagtcacc gcggtatcag gcagcgtgat cacggcgcgc accgagtggg gcgcaagcgc
      181 actcacctgg accgtagaca cgaatggcga cacggacttc gtatcccgcg gaaaccaaaa
      241 tcttgatatc gacgatatcg atattggtga taccgtgagc ttctcaggcg cgctcgatgt
      301 cgacggctcc gcgttcaatg tggatgctga tgtggtgaag aactggtcgc ttgatacgaa
      361 cgacacccgc gaggctcgcg acgaggctcg cgtcgaagcg aagaccgagc tcaaggctaa
      421 gtggggcgat tggatgcgcg gcatcccgct tatgaactgg ttcaacaagc acgataaccg
      481 ctagcgcttt gaaaaacccc ggctcacaga gccggggttt ttctgttagt atcgcgatat
      541 ggaagagacg ctctcgcttg ctatacggac cgcacttgat gagatgggtg cggagaacgc
      601 actgttcacg atcgagtggc cggcggatct cgcgcatggg gatttcgcta cgaatgccgc
      661 actcgccgct gcaaagcctc ttggcaggtc tccgcgcgag attgcagatg agctcgcggg
      721 aaagcttcgc gatgtgcttg gcgagcaggt cgcttcgatc gaggtagcag gtcccggatt
      781 cgtgaacgtc acgctcgcac gcgacgcgat agcgggtgcg atacggagcg tgccagctga
      841 agggtggggt agcggaagtg ctcgttctga agaacggatt gcattcgagt actcctgccc
      901 gaatccgttc aaggagatgc atgtagggca tctcatgagt acggttatcg gggaagcggc
      961 gtcacggctc attgagaacc agggagcacg cgtgcttcgc gagacgtacg gcggcgatgt
     1021 gggaccacac gtggcaaagg cactctgggc actcagaagg aagggtaatg acgaaccggc
     1081 gacaccagaa gagatcggtg aggcctacgc acagggaagt cgcgcgtacg aggagagcga
     1141 ggaggcgaag gcagagatcg acgcgcttaa cgtgtccttg taccaagcgc ttgcaaagga
     1201 agaaggagag cgcagcgacg aggatcgcac actccttgag ctttggcggc acgggcgcga
     1261 cgcgtcactt gcggctcata aggcggtatg ggacgcgctt ggtacccatt ttgattacgt
     1321 tttccacgag agtgaaacca ctcctattgg catggaggta gtgacgcgcg cacgtgaaga
     1381 aggagtattc gtcgagagtc agggagccgt tatctatgac ggtgagaaga aggggctgca
     1441 cacgcttgtg ttcctcacgt ctcgtggcac gccgacgtac gaagcgaaag agatggggct
     1501 tgcgttcctg aaggaagaac gcattggccc gctcgatcac gcgtacatca ctaccgctgc
     1561 agaacaaaag ggacatttcg aggtattcct cgcagccctc acagacgtcg cgccagagct
     1621 cgcacagaaa atcgagcatg taccacacgg attcctcaga cttacgaccg gaaagatgtc
     1681 gtctcgcgaa gggaacgtga ttaccggcgc cggcctcatt agcgagataa tcgataccgc
     1741 gcgtgagaag aatcaggatc cgtttgtggc gaaggccgta gctatagctg ctattaagta
     1801 catgattctg cgtcaggctg cgggcggcga tattattttc gaccccgaga agtctctttc
     1861 gcttgatgga gattccggac catacttgca gtacgcgctc gtgcgcgcac gatccgtgct
     1921 cgcgcaggcg aagaagccag agaacatcaa ggcgcccctg gagccgtacg cacttgagcg
     1981 actcatcatc cggttcccgg aggtagcgcg tcgtgcagaa gcggagctcg cgccgcataa
     2041 ggtcgcgcag tacctcacgc aactcgccgg ggaatggaat tcgttctacg cgaataaccg
     2101 catcatcggc ggcgagcatg aaggctacaa gcttctcctt gcgcgcgcgt ttgcgctcac
     2161 gatggagcaa ggtctcaacc ttctcggtat tcccgtgccg gagcgaatgt agataagacg
     2221 cttgcgggtt tctatattcg ggggtagggt agcgcttgtc tccatggggg aggcatgaaa
     2281 gatccaagga ggacgctttg tccaaaagaa tcaaggtcag caggaaacgc atggatgttc
     2341 tggcgcagct ggcgcgggag cgccgtatga agatcgatga gatcttctgg gcctgcgccg
     2401 cgtggcttcg ggcccggggg gaacgcgtga agaacttcac gatcaggaac tggctgagtc
     2461 gctacaagca gacccgccgc atccacccca aggtggccca cttcgcccag catctccagg
     2521 ccgcgtccgc ctgaggtcct gaacctggtt caacggtccg cgcttgcgcg ggc
//
LOCUS       DASWXU010000039         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_342634, whole genome shotgun sequence.
ACCESSION   DASWXU010000039 DASWXU010000000
VERSION     DASWXU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342634"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..2117)
                     /locus_tag="VGE48_01355"
     CDS             complement(201..2117)
                     /locus_tag="VGE48_01355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY1042049.1"
                     /translation="MSAAPAPAPAPSSRPKPDARSERPEGAPSRPRGARSRGTRGGQG
                     RARGGRASRDIRRTLGRPGGNDAPREKKQAEYLPAAPGKDTVRIVPLGGVEEVGRNMF
                     TVETNGDIFVFDVGFQFVSEDTQPGVDYVLPNTKYLEVNKDRIRGVFITHGHLDHIGG
                     IPFLMERMGNPTIYTRTLSATMIKKRQEEYPDQAPLNIVEVEAGQQMTIGSTKVRFFQ
                     VTHSIPDSMGISVETPYGDVVATGDLKLDHDNGIPTEREIKVWGELGSRKNLFFIADS
                     TNAERDGFSVPEKKVHETLEEIVRTVKGRLIIGTFASQFERMVRIIEICESLGKKVVT
                     EGRSIKTNLEIAQKTGLLKVKKETIIPSQEIGDYPPDKVVILSTGAQGEEFAALMRIA
                     TKQHKSITLTPRDTVVLSSSVIPGNEISVQRLKDCLYRNGVTLIHYKSSDVHSTGHGN
                     TGELVWMNKQVNAKYFMPAYGYYSMTYSHAKAVEQAGRPRESIVVADNGTVIDIGPDE
                     KLNVHKQKVPSSPLVVDGYSIGDFQEVVMRDRQMLAKDGMFVIIATVNLKTGKLRKSP
                     DIISRGFVYLRENQEMLNQARLLIKKTVEDTTQGMNPVDLDEVKNALADTMAGFLFMR
                     TNKAPMVIPVLIGV"
     gene            complement(2221..3918)
                     /locus_tag="VGE48_01360"
     CDS             complement(2221..3918)
                     /locus_tag="VGE48_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1042050.1"
                     /translation="MRSSPFYVPDQSNSPLIKAQRFLRSLSPGDRAIAAVLGLLLVAS
                     LFSGLVALIRAYQVTVPAYGGSLTEGVIGSPRFINPLLALSDADRDLTSLVYAGLMGV
                     DGDGNMVPVLAERYLVSEDGTEYTFLLRENAKFSDGSPVTAEDVVFTVERAQDPALRS
                     PELANWANIRAEAVDARTVRFTLPQPYAPFLADTTLGILPAKLWRNVPVDEFSFSPQM
                     TEPVGAGPFKVSRVTKDKAGKITSMELSVFKDYAPGRAYLNRITMKFYQDQEALKDAY
                     DGRQIESAYGILAKDMLRAPYSRVFGTFFNSDENPVFAKLEVRKALSFAVNRARITQD
                     LLGGNATPLMGPVPPGSGVTPFPVPEGDPVALATEVLTDAGWERDEETGLWGDGETIL
                     SSVTIKTANVPELKAIALSIEADWEALGVPTEVELYEPGDLASSVIRPRAYEALLFGM
                     VIGRDRDLYAFWDSGERSDPGLNISAYANRAVDQLLESARQELDPEIARQDLEELSTL
                     IAEDYPAAFTHAPDFLYAIPNSVHGVRLPQIAAPSDRFATVAHWYRNTEEVWPVFAK"
BASE COUNT          847 a         1150 c         1252 g          842 t
ORIGIN      
        1 atcacgagtc ctacctgtga ctctggcagg aagagtgcta ggtacggcgc tgcgatgaca
       61 agcacaagcg cgaaatacat cgcgctgaag aagttgcctg tggaaagaac gagacgggga
      121 tgcatcgaac gaattctagc attcaggaaa gagaaaatgc cgcatcgctg cggcattttc
      181 tgtgcatgag gcgtctttcc ttacacgccg ataagaaccg ggatcaccat aggcgccttg
      241 ttagtcctca tgaagaggaa gcccgccatg gtatctgcaa gcgcgttctt cacctcgtcg
      301 agatcaactg ggttcatgcc ctgtgtggtg tcttccacgg tcttcttgat gagaaggcgt
      361 gcctgattga gcatctcctg attctcgcgc aggtatacga atccgcgaga aatgatatcc
      421 ggagacttgc ggagctttcc ggtcttcagg ttcacggtcg cgatgatgac gaacatgccg
      481 tccttcgcga gcatctggcg atcgcgcatc acgacctcct ggaagtcgcc gatggagtag
      541 ccgtccacca cgagtgggga agacggcacc ttctgcttgt gcacgttgag cttctcgtca
      601 ggaccgatat cgatgacggt accgttgtct gctacaacaa tggattcacg cgggcgtcct
      661 gcctgctcga ccgccttcgc atgcgagtag gtcatggagt agtagccgta ggccggcatg
      721 aagtacttcg cgttgacctg cttgttcatc cacacgagct cgccggtgtt gccgtggccc
      781 gtggagtgca cgtcggaaga cttgtagtgg atgagggtaa cgccgttgcg atagaggcag
      841 tccttaaggc gctgcacgga gatctcgttg cccggaatga ccgaggacga aaggaccacg
      901 gtatcgcgcg gcgtaagcgt gatcgacttg tgctgcttgg tcgcgatgcg catgagcgcg
      961 gcgaactcct cgccctgagc gcccgtcgag aggatcacta ccttgtctgg cgggtaatcg
     1021 ccgatctcct gggaaggaat gatcgtctcc ttcttcacct tgagaagacc cgtcttctgg
     1081 gcaatctcga ggttcgtctt gatggagcgg ccttcggtca cgactttctt tccgagagac
     1141 tcgcagatct cgatgatgcg gaccatacgc tcgaactgag acgcgaaggt accgatgata
     1201 aggcggccct tcacggtgcg tacgatctcc tcgagggtct cgtgcacctt cttctcaggg
     1261 accgagaatc cgtcgcgctc tgcgttggta gagtcggcga tgaagaagag gttcttgcgg
     1321 cttccgagtt cgccccagac cttgatctcg cgctcggttg gaatgccgtt gtcgtgatca
     1381 agcttgaggt ctccggttgc gacgacgtcg ccgtacgggg tctcaacaga aatacccatg
     1441 gaatccggga tggagtgcgt tacctggaag aagcgaacct ttgttgagcc gatggtcatc
     1501 tgttggccgg cctcaacctc aacgatattg agaggagcct ggtccgggta ctcttcctgg
     1561 cgcttcttga tcatcgttgc cgaaagggtt cgggtataga tggtcgggtt gcccatacgc
     1621 tccataagga atgggatgcc gccgatgtgg tcgaggtggc cgtgagtgat gaatacgccg
     1681 cggatacggt ccttgtttac ctcaaggtac ttggtgttcg gaaggacata gtcgacgccc
     1741 ggctgcgtgt cttcggacac gaactggaag ccgacgtcga agacgaagat gtcgccgttg
     1801 gtttcaacgg tgaacatgtt gcgtcccact tcctcaacgc ctccaagggg cacgatgcgg
     1861 accgtgtctt ttcctggggc tgcgggaagg tattccgcct gcttcttctc gcgaggggca
     1921 tcgttgccac ctggacggcc gagtgtgcgg cggatatcgc gcgatgcgcg gccgccacga
     1981 gcacgaccct gtccaccgcg ggtgccgcgg ctacgagcgc ctcgtgggcg tgatggtgcg
     2041 ccctcaggac gctctgaacg agcatctggc tttgggcggg agcttggcgc cggagccgga
     2101 gcgggtgctg ctgacataat gattgatagt tatctaatag tttcttttca atgaagcgca
     2161 cgtatgcgca gtaggcagcc tgtatgccga tgtgggcctc taggctatcg actgttcaca
     2221 ctacttcgcg aagacgggcc agacctcctc cgtgtttcgg taccaatgcg cgacggtcgc
     2281 gaatcggtcc gacggggctg ctatctgggg gagacgtacg ccgtggacgc tgttagggat
     2341 agcatacagg aagtccggtg cgtgcgtaaa ggcagcagga tagtcttctg cgatcagggt
     2401 agaaagctcc tcaagatcct gacgcgcaat ctcaggatca agctcctgac gcgcgctctc
     2461 aaggagctga tccaccgcgc ggttcgcata tgcagagata ttaagaccag gatcagagcg
     2521 ctcgcccgag tcccagaacg catagagatc gcgatcacgg ccgataacca tgccgaagag
     2581 cagggcttcg tacgcgcgcg gtcggatcac ggagcttgcg aggtctcctg gctcgtaaag
     2641 ttcgacttcg gtaggaacgc caagcgcttc ccaatctgct tcgattgaaa gcgcaatcgc
     2701 cttgagctca ggcacgttcg cggtcttgat cgtaaccgac gagagaatcg tctcgccgtc
     2761 tccccagagt ccggtttcct catcacgctc ccatccggcg tccgtaagga cctcggtcgc
     2821 gagcgctaca ggatccccct caggaacagg gaagggagtt acgccgcttc ctggcggcac
     2881 cggacccatc aaaggcgtcg cgttccctcc gagaaggtcc tgcgtgatgc gcgcgcggtt
     2941 aaccgcaaag gaaagcgcct tacgcacctc aagtttcgcg aacaccggat tctcgtcact
     3001 gttgaagaac gttccgaaca cgcgggagta cggagcacgg agcatgtcct tcgcgaggat
     3061 gccgtacgcg ctttcgatct gcctaccgtc gtacgcgtcc ttgagtgcct cctgatcctg
     3121 atagaacttc atggtgatgc ggttaaggta cgcgcgtcct ggtgcatagt ccttgaatac
     3181 ggaaagttcc atcgaggtta tcttcccagc cttgtccttc gtcacacgag aaaccttgaa
     3241 gggccccgcg cctaccggct ccgtcatctg cggcgagaac gagaactcgt ccacgggcac
     3301 gttcctccag agcttcgccg gaaggatgcc aagcgtggtg tctgcaagaa acggcgcgta
     3361 tggctgcgga agcgtgaagc gcacggtgcg cgcatccacc gcttcagcgc ggatgtttgc
     3421 ccagttcgcc agctccggag aacggagcgc cgggtcctgc gcgcgctcga cggtgaacac
     3481 cacatcctca gctgttacgg gagagccgtc tgagaacttc gcgttctccc tgaggaggaa
     3541 cgtgtattcg gtaccgtcct cagacacgag atagcgttca gcaagcacgg gaaccatgtt
     3601 cccgtcccca tccacgccca tgagccctgc atacaccaag gacgtgaggt cgcggtcagc
     3661 gtcagagagc gcgagcaggg gattgatgaa acgaggtgac ccaatcacac cctcggtaag
     3721 gcttccgcca tacgccggca ccgtcacctg gtacgcgcga ataagcgcaa cgagccctga
     3781 aaagagagac gcaacgagca agagcccgag cactgccgcg atcgcgcggt ctcctggcga
     3841 aagggaacga aggaagcgct gcgcctttat aagaggcgaa ttgctctgat caggtacgta
     3901 aaaaggggag gaccgcatag gcggaatagg gcacagatgg cgcccagaaa agcaggctag
     3961 tagaagaggc ctgcgactgc agctgccacg aagaggatag cgataacgat ggtcgcgttg
     4021 aacaggaact tctcagcgcc gcggcgcttg tagaaggtag acgcgaagtt gtcgcctccg
     4081 aaggctccac c
//
LOCUS       DASWXU010000040         8407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_747963, whole genome shotgun sequence.
ACCESSION   DASWXU010000040 DASWXU010000000
VERSION     DASWXU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747963"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGE48_01365"
     CDS             complement(<1..213)
                     /locus_tag="VGE48_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042051.1"
                     /translation="MERVSDTMVQAMLDLGALVFAFAKTDRITRHPDGVSLESDTDHT
                     VMLGIAACAFASRLAPHLDIGKIAQFA"
     gene            complement(216..845)
                     /locus_tag="VGE48_01370"
     CDS             complement(216..845)
                     /locus_tag="VGE48_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY1042052.1"
                     /translation="METTIFTVIVLILSVVIHEVAHGWAANALGDPTARLQGRLSLNP
                     VRHIDPIGSVLIPAVLVLTNSSFLFGWAKPVPYNPYNLKNQRWGEAIVGVAGVATNLL
                     LAVIFALIARFAFGAGMTEFGTLASLVTLVNLSLGLFNLLPIPPLDGYTVLRGVLPYR
                     ASLSLRALEDKVAAGGIMSLVLILFLFSFFFAAPFGMLVRSVFAFLIGA"
     gene            892..1137
                     /locus_tag="VGE48_01375"
     CDS             892..1137
                     /locus_tag="VGE48_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HEY1042053.1"
                     /translation="MARECAVTGKSTQIGGRYSNRTRATQFNPTGKVRRQANLQKRRI
                     FVPELAKTILVDISAKGMRHIKKNGAFATLKKAGVIK"
     gene            complement(1168..2034)
                     /locus_tag="VGE48_01380"
     CDS             complement(1168..2034)
                     /locus_tag="VGE48_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1042054.1"
                     /translation="MRTRIPKPALALLIALLLVGNALLFTAILRPDTSTLAVAFLDVG
                     QGDAIYIESPTGVDLLIDGGKDKSAVRELSKVMGPLDRRIDIVLATHPDADHIGGLPD
                     VLSRYRVGTVLESGKAGDSSQADRFTDSIRREAGAAYGIAKQDMRIHLGEGVYADVLF
                     PEGDAKKLRETNDASVILRLVYGESEFMLTGDAPSWVEERLVQKYGRGLRSDVLKAGH
                     HGSRTSSSDVFLFAVMPQTVVVSAGKDNPYGHPHPEVLDNVREIGATVVSTAESGTIV
                     FRSDGKSVTRVR"
     gene            2100..2690
                     /locus_tag="VGE48_01385"
     CDS             2100..2690
                     /locus_tag="VGE48_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY1042055.1"
                     /translation="MTYPVRTFDLPELTGLSKDQIAVHLALYEGYVKHTNLILDTIKK
                     LREENAEGNAFQINELRRRLGFEFDGMRMHEYYFEQFEGGSKVASAEGNLRTAADEKY
                     GNWDGFIAHIKEVAGTRGIGWTVVYFDTPNGGLHTAWVDDHQLGQFSGVPVLLALDMW
                     EHAFMVDYKPAEKKTYVEAFLDNLNWEVVERRLARC"
     gene            complement(2836..3258)
                     /locus_tag="VGE48_01390"
     CDS             complement(2836..3258)
                     /locus_tag="VGE48_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042056.1"
                     /translation="MRSNARTAKTFDQRLREFLAYRGNQDDIEIAVAVELYLTNPNLG
                     HEAAFKDALEILKYQKEVMPIIEPSDSDPRRATITFAVPGGGQIQMRLHDGVEGCALR
                     STKGRLPERKACGREIKRISSGSEFLKLIAARRRSMSR"
     gene            complement(3331..3747)
                     /locus_tag="VGE48_01395"
     CDS             complement(3331..3747)
                     /locus_tag="VGE48_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HEY1042057.1"
                     /translation="MRFTIYADGGSRGNPGPAGSGAIIRNESGETVATVSEYLGVSTN
                     NVAEYTAVLRALEKLAAMLESTSKEADVEVRMDSMLVVRQMSGEWKIKHENMKPLAAS
                     TNEVIQRFRSVSFAHVYREDNKDADKLANEAMDRGH"
     gene            complement(3764..5506)
                     /gene="recJ"
                     /locus_tag="VGE48_01400"
     CDS             complement(3764..5506)
                     /gene="recJ"
                     /locus_tag="VGE48_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY1042058.1"
                     /translation="MFTLHDPLDESVREALSSYDDLVAALLARRGLKTKEEAEAFLSP
                     SYDEGIHDPMLMTDMPKAAERLARAIGSSEKIAVWSDYDCDGIPGGVLLHDFLKKAGA
                     NFVNYVPHRHNEGYGMNIPGIEQLKKEGVSLIVTVDSGITDSAQVAHANSLGIEVIVT
                     DHHLPSAEGLPPAYAVLNPNAREDETYPYKSLCGAGVAWKLACATLAHGFPGRDTIPE
                     GWEKWLLDMAGMATIADMVPLTGENRVIASYGLLVMRKSPRIGFQKLCRQARVDQKRI
                     TEDDIGFMIAPRVNASSRMGDPRDAFELLSTTDEKKADELSRKLESLNRSRRAAAGAI
                     TRAVHDRLLERKAEGKELPLVIAMGDPDWRPGLLGLVANGIAEEYGRPVFLWGREGNG
                     ANKGSCRSGPGNVSVGSLMAAAGDTFDQFGGHRASGGFTVASHAIFDLEERLARALAS
                     LPEEDAEEETRADALLSPESVTKELLRNLSRLAPFGMGNPRPVIAFHEVRVEQVSWFG
                     KANEHLRLAIGRGHEEFPEAPLEAMTFYAKRQLGEVCERIEKGQQISLLAHIEEDTFT
                     RGQPMRLRIVSVRA"
     gene            5554..5976
                     /locus_tag="VGE48_01405"
     CDS             5554..5976
                     /locus_tag="VGE48_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018073450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY1042059.1"
                     /translation="MELHGENGVFMKIVRRELPAHIVYEDEKTLAFLSTGPVEPGHTL
                     VIPKTPFRNVFDIDQEYFAAVMETVRKIAPAIRDAVGAKGVHINSNHEPEAGQEVFHL
                     HMHIVPRHERQSFEFWPNTKYEDGEAEAVAEKIRNELA"
     gene            complement(5968..7101)
                     /gene="pgk"
                     /locus_tag="VGE48_01410"
     CDS             complement(5968..7101)
                     /gene="pgk"
                     /locus_tag="VGE48_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY1042060.1"
                     /translation="MRTVRDIRKLEGIPVLVRAALNAEAENGRVTNDFRLRAALPTIE
                     YLRERHAKVILAGHITGNGTETLRPMYEAMKVWIPGLVFCDVPSGPKARAMARELSPG
                     GVLMLENLRRDKGEEGNSKEFAASLADLADIFVQDAFDVCHRKHASVVGVPEILPSYV
                     GLLLAHEIEELKKALKPKQPSMAIIGGAKFSTKQPVLNKLLKIYGRVFVGGALANDFM
                     QATGRPVGRSLVSNADKITLKALLQDRKLLLPLDYVVAPVGKGRAEGRIAEIQNVRPD
                     EAILDNGPKTIAMLAEHAKSMKTILWNGPLGNYEHGFVEGTQGLARVIAASHAHSIVG
                     GGDTVAAIESLRLSQGFSFISTGGGAMLDYLAYGTLPGIAALR"
     gene            complement(7104..7853)
                     /gene="tpiA"
                     /locus_tag="VGE48_01415"
     CDS             complement(7104..7853)
                     /gene="tpiA"
                     /locus_tag="VGE48_01415"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY1042061.1"
                     /translation="MLIVGNWKAYVETRAKAKTLYQGAKRLAAKGTHEIVVAPPFPYI
                     GMLAPAKAGKTDPGLASQDVSITVGGAQTGEVAAGLLRGLGVSFVIVGHSERRARGED
                     DATVLEKARHALAHGMTPIICIGERERDDEAHYLKDVRRQVEAIFAHLSPKERLQVVL
                     AYEPIWAIGKTGADAITPTDLGEMVLYIRKVLAGHMPGRANQKVRILYGGSVEATNVR
                     GLAGGSGVDGFLVGHASTDVAGFSALVKALS"
     gene            complement(7877..>8407)
                     /locus_tag="VGE48_01420"
     CDS             complement(7877..>8407)
                     /locus_tag="VGE48_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042062.1"
                     /translation="YQTLTLPSDCALIFVEDHVWIEGIVSQKVTVVSATPTLTNVKTN
                     VMLPGNLRYAATDGSDGLTVISENNILITPNSPQNMTLNGIFVAQGGSFGRNRYACPS
                     SYEPRTSLTILGTTVSNMRTGTQWQNGCWSLFGGYSDAGYQSRVDSFDRKLATDPPPF
                     TPQTSTDYQFVDWREE"
BASE COUNT         1838 a         2540 c         2274 g         1755 t
ORIGIN      
        1 cgcgaactgc gcgatcttgc cgatatcaag atgcggggcg agccggcttg cgaacgcaca
       61 cgccgcgata ccgagcataa ccgtatggtc cgtatccgat tcgagcgaca cgccgtccgg
      121 atggcgcgtg atccggtcgg tcttcgcgaa cgcaaagaca agcgcgccca agtcgagcat
      181 cgcctggacc atcgtgtcag atactcgctc catacctacg cgccaataag gaacgcgaac
      241 acactgcgca ccagcatgcc aaagggcgcc gcaaagaaga acgagaataa gaagagtatg
      301 agaacgaggg acatgatccc gcctgccgct accttgtcct caagcgcccg aagcgaaagg
      361 ctcgcacggt acggaaggac gccgcgaagg accgtatatc cgtcgagcgg tgggatcggg
      421 agaagattga agagaccgag cgaaagattc acgagcgtaa ccagggatgc gagtgtccca
      481 aactcagtca tgcccgcgcc gaacgcaaag cgagcgatga gcgcaaagat aacggcaagg
      541 aggagattcg tcgccacacc cgcgacgcct acgattgcct cgccccaccg ctgattcttc
      601 aggttatacg ggttgtacgg gaccggcttc gcccaaccaa agaggaacga ggaattcgta
      661 aggaccagta ctgccgggat aagcaccgag ccaatcggat ctatatgacg gaccggattg
      721 agggacaggc ggccctgaag gcgcgcggtg gggtccccga gggcgtttgc ggcccatcca
      781 tgggctactt catggataac caccgagaga atgaggacga taacggtgaa aatggtggtt
      841 tccatagaaa agttgcctaa aaacgtatgc gtatgctagc atgcggggac tatggcacgc
      901 gaatgcgcag taaccggaaa gtcgacccag attggcgggc ggtactctaa ccgtacccgt
      961 gctactcagt tcaacccaac cggaaaggta cgccgccagg cgaacctcca gaagcgccgc
     1021 atcttcgtac ctgagcttgc gaaaaccatc ctcgtggaca tcagtgctaa gggcatgcgc
     1081 cacatcaaga agaacggtgc cttcgctact ctcaagaagg caggcgtcat taagtaagaa
     1141 aagagctcca gaaacggagc tcttttctta tcgcacccgc gttactgact ttccgtcgct
     1201 tctgaagact atggtcccgc tctcggcggt tgatacgacc gtagctccaa tctctcgaac
     1261 gttgtcgagt acttccgggt gcgggtgccc atacggattg tccttccctg ccgacacgac
     1321 caccgtctgg ggcatgaccg caaaaagaaa cacgtccgaa gaagaggtgc gcgatccgtg
     1381 atgtcccgcc ttaagcacat cgctccttag tcctctaccg tacttttgta ccagccgctc
     1441 ctctacccac gaaggcgcat ccccggtaag catgaattca ctctctccgt acacgagccg
     1501 gagaataacg gacgcgtcgt tcgtctcccg aagctttttt gcgtccccct caggaaacag
     1561 cacatccgcg tatacaccct ctcccagatg gatgcgcatg tcttgcttag caatcccgta
     1621 tgccgcaccc gcttcgcgcc ggatcgaatc ggtgaaccga tcggcctggc tcgagtcccc
     1681 agcctttcca gactcaagga cggtgcctac gcgataccgc gaaagtacgt ccggaagccc
     1741 gcctatgtga tcggcgtccg gatgtgtcgc gagcacgatg tcgattctcc gatcgagcgg
     1801 tcccatgact ttcgaaagtt cgcgtaccgc tgacttatcc ttcccgccat ctataagcag
     1861 gtcgacgcct gtaggacttt cgatgtatat cgcgtctccc tgacccacat cgagaaacgc
     1921 tacggcaagc gtgcttgtgt caggcctgag aatagcagtg aagaggagcg cattccccac
     1981 caagagtagc gctatcagaa gcgcgagcgc aggcttaggg atccgggtac gcatgctccc
     2041 gatatcatcg catgttcacg accaggcggt atactgcctt caagctaacg aatctatcca
     2101 tgacctatcc cgtccgcacc tttgaccttc ccgaattaac cggcctttcc aaggatcaga
     2161 tcgctgtgca ccttgcgctc tacgaaggct acgtgaagca cacgaatctc atcctcgaca
     2221 ccattaagaa gctccgcgag gagaacgccg aaggaaacgc gttccagatt aacgaattga
     2281 ggcgccggct cggattcgag ttcgacggca tgcgcatgca cgagtactac ttcgagcaat
     2341 tcgagggagg ttcgaaggtt gcgagcgccg agggaaatct acgtaccgca gccgacgaaa
     2401 agtacggaaa ctgggacggc ttcatcgcac acataaagga ggtggctggg acacgcggca
     2461 tcgggtggac cgtcgtgtac ttcgatacac ctaacggagg ccttcacacc gcatgggtag
     2521 acgatcacca gctcgggcag ttctcaggcg taccagtgct tcttgcgctc gatatgtggg
     2581 agcacgcgtt catggttgac tataagccgg cagaaaagaa gacctacgtg gaagccttcc
     2641 tcgacaacct gaactgggaa gttgtcgagc gcagactcgc tcgctgctag ataaattttt
     2701 aaagtcaccg gccacttaca gtggccggtg actttttatt taaacgagaa acgagcggca
     2761 tgctatgccg ctcgcaaatc ccaaaaccag agagaagagg agcgttctgc tcatatgccg
     2821 atatactatc agcccctacc tcgacatact tctgcgcctg gccgcgatga gctttaggaa
     2881 ttctgaacct gaactgattc tcttgatttc cctgccgcac gcctttcgtt ccggaagcct
     2941 tcccttggtc gaacgaagag cgcacccttc gaccccatca tgcagtcgca tctgaatctg
     3001 accaccgcca ggaactgcga acgtgatggt ggcgcgcctt gggtcagagt cagaaggttc
     3061 gatgatcggc atcacctcct tctgatactt cagaatctcc agggcgtcct tgaaagctgc
     3121 ctcgtgaccc aaattggggt tcgtgagata cagctcgacg gcgacggcga tttcgatgtc
     3181 gtcttgattc cctctatacg caaggaactc ccgaagacgt tgatcgaagg tttttgctgt
     3241 acgagcgtta gaacgcatta ctctctccct aagaacagtc ttccggctga agccggttgt
     3301 ttctgaccct acgcctcaaa acaagaggcg tcaatgtccc ctatccattg cctcgttcgc
     3361 gagcttgtca gcgtctttgt tgtcttcgcg gtatacgtgc gcaaatgaga cggagcgaaa
     3421 acgctgtatc acctcatttg tgcttgcagc tagcggcttc atattctcgt gctttatttt
     3481 ccattctccc gacatctgcc gcaccacgag catgctatcc atgcgcactt cgacatctgc
     3541 ttccttggac gtggactcaa gcatcgctgc gagcttctcg agcgcgcgaa gcaccgcggt
     3601 gtactcggca acattgttcg tggagacgcc gagatactcg gaaacggtag cgaccgtttc
     3661 tcctgattcg ttccggataa tcgcaccgga acctgcaggg ccagggttcc ctcgagaacc
     3721 gccatcggca tagatagtga aacgcatgga gataagtata gcgtcaggcg cgcacggaca
     3781 cgatccgaag gcgcatcggc tgcccgcgcg tgaaagtgtc ttcctcgata tgagcaagga
     3841 gcgagatctg ctgtcccttc tcgatgcgct cgcacacctc tccgagctgc cgcttcgcat
     3901 agaaggtcat ggcctcgagc ggagcttcag gaaattcctc gtgtccccga ccaatcgcaa
     3961 ggcgcagatg ttcatttgct tttccaaacc atgacacctg ctcgacgcgc acctcatgga
     4021 acgcaatgac cggccgcgga ttacccatac caaacggtgc gagcctgctc aggttccgta
     4081 acagctcctt agttacggac tctggagaca aaagtgcgtc agcacgcgtc tcttcttccg
     4141 cgtcttcttc tgggagcgac gcgagcgcac gcgcgagccg ctcctcgaga tcaaagatgg
     4201 catgcgacgc taccgtgaac ccgcctgacg cacgatgtcc gccgaactga tcgaacgtat
     4261 ctcctgccgc tgccatcaag gaaccaacac tcacgttgcc cggaccgctc cggcacgatc
     4321 ccttgttcgc gccgtttcct tctcgtcccc ataaaaatac cggccggccg tattcctcgg
     4381 cgatcccgtt tgctacgagc ccgagaagtc ccggccgcca atccggatcc cccatcgcga
     4441 taaccagagg aagctccttg ccttccgcct tccgctcgag cagacgatca tggactgcgc
     4501 gcgtgatagc acctgcggcc gcgcgacgag aacggttaag cgattcaagc ttccgggaga
     4561 gctcgtccgc cttcttctcg tcagtggtcg aaagcagctc gaacgcgtct cgcggatctc
     4621 ccatgcgcga cgacgcgttc acacgaggtg cgatcatgaa tccgatgtcg tcctcggtga
     4681 tcctcttctg atcgacacgc gcctggcgac atagcttctg gaacccaatg cgcggggact
     4741 tccgcataac gagaagtccg tacgaggcga tgacgcggtt ctcccccgta agcggcacca
     4801 tgtcggcgat cgtcgccata cccgccatat caagaagcca cttctcccat ccttccggta
     4861 tagtgtcgcg tcctgggaac ccgtgcgcca aagtcgcgca tgcgagcttc catgcgaccc
     4921 ctgcaccaca gagcgatttg tacgggtacg tctcatcctc gcgcgcgttc gggtttagca
     4981 ccgcgtacgc aggcggaagc ccttctgcgc tcgggaggtg atggtcggtc acgatgacct
     5041 ctatgccgag actgttcgcg tgggccacct gcgcgctgtc ggtgatacct gagtcaaccg
     5101 tcacgatgag cgacaccccc tcctttttga gctgctctat gcctgggatg ttcatcccgt
     5161 acccttcgtt gtgccggtgc ggcacatagt tcacgaagtt cgcgcctgcc ttcttcagaa
     5221 agtcatgtag gagcacgcca cccggtatcc cgtcgcagtc gtaatcgctc catactgcga
     5281 ttttctcgct actcccgata gcgcgagcaa gacgctctgc ggctttaggc atatcggtca
     5341 tgagcattgg gtcatgaatg ccctcgtcgt aggacggaga aaggaatgct tctgcttctt
     5401 ccttggtctt cagacccctg cgcgcaagga gcgctgcgac gaggtcgtca tatgaagaca
     5461 gcgcctcgcg aaccgactca tccaaaggat catggagcgt gaacatgttt gcgcattgta
     5521 tcaaaatcgg cgctcgcggt atccttgacg gatatggaac ttcacggaga gaacggggtg
     5581 tttatgaaga tcgtgcgccg ggaactgcct gcgcacatag tctatgagga cgagaagaca
     5641 ctcgcattcc tttcgaccgg acctgtggag ccgggacaca cgctcgttat cccgaagaca
     5701 ccgttccgca acgtcttcga tatcgatcag gaatattttg ccgccgtcat ggaaaccgtg
     5761 cgtaagatcg ctccagcaat acgcgatgcg gtaggcgcca agggcgtaca cattaactca
     5821 aaccatgagc ctgaggcggg acaggaagtg ttccaccttc atatgcatat tgtcccgcgc
     5881 cacgagcgac aatcgtttga attctggccg aacaccaagt acgaagatgg cgaagcagaa
     5941 gcggttgcgg agaaaatccg caacgagcta gcgtagcgcc gcgatgccgg gaagcgtgcc
     6001 gtatgcgaga tagtcgagca tcgcgccacc gcctgtggag atgaacgaaa agccttgaga
     6061 aagcctgagg ctctcgatag ccgctaccgt atcgccgcct ccgacaatcg agtgcgcgtg
     6121 cgatgccgcg atcacccgcg caagaccctg cgtaccttcg acgaacccgt gttcatagtt
     6181 tccaagaggc ccgttccaga gaatggtctt catacttttc gcatgctccg cgagcatcgc
     6241 gatggtcttc ggtccgttat caagaatcgc ctcgtccgga cgaacgttct ggatctccgc
     6301 gatgcgtcct tctgcgcgac ccttccctac gggagcgaca acataatcaa gcggcaagag
     6361 gagctttctg tcctgaagca gcgctttgag agttatttta tctgcgtttg ataccaagga
     6421 cctccccacg ggacgaccgg ttgcctgcat gaaatcgttc gcgagcgccc cgcccacgaa
     6481 cacgcgtcca tagatcttga ggagcttgtt tagtaccggc tgcttcgtgg agaacttcgc
     6541 gccaccgatg attgccatcg atggctgctt cggcttgagc gcctttttca attcctctat
     6601 ctcgtgcgca agcagcagcc ctacatagct cgggagtatc tctggcaccc ccacaaccga
     6661 ggcgtgcttc cggtgacaga catcgaacgc atcctgtacg aaaatatcag ctaggtccgc
     6721 gaggcttgca gcgaattcct tactgttccc ttcctccccc ttgtcgcgtc tgagattctc
     6781 aagcatcagc acgccgccag gtgaaagctc tcgtgccatt gcgcgcgcct taggtcccga
     6841 tggcacgtcg cagaatacga gtcccggaat ccagaccttc atcgcctcgt acataggacg
     6901 gagggtctcc gtgccgtttc cggttatatg acccgcgagg ataaccttgg cgtggcgctc
     6961 gcgcaggtac tcgatggtag gaagcgcggc acgcagtcgg aagtcgtttg tgaccctccc
     7021 attctccgct tcagcgttaa gcgcagcgcg cactaagacc ggaatgcctt cgagcttccg
     7081 aatgtcacgt actgtacgca ttgctagctg agcgctttta cgagcgcaga gaacccagcg
     7141 acatcagtgg atgcatgtcc cacgaggaac ccatcgacgc cacttccgcc agccagtccg
     7201 cgaacattcg tcgcctcaac cgagccacca tagaggatgc gtaccttctg gttcgcgcgc
     7261 cctggcatgt gtcctgcgag caccttcctg atgtagagca ccatctctcc aaggtcggtg
     7321 ggtgtgatcg catcggcccc ggtcttgccg atggcccaga tcggttcgta tgcgagcaca
     7381 acctggagac gctccttcgg ggaaagatgt gcgaagatcg cttcgacctg tcggcgaaca
     7441 tccttcaaat agtgcgcctc gtcatcccgc tcgcgctctc ctatacaaat gatcggtgtc
     7501 ataccgtgcg cgagcgcatg gcgcgccttc tccagaactg tcgcgtcgtc ctctccgcgc
     7561 gctctacgct ctgagtgtcc gacgatgacg aacgagaccc caaggcccct aagaagccct
     7621 gccgcaacct ccccggtctg cgcgccgcct acggttatag aaacatcctg agacgcgagt
     7681 ccggggtctg tctttccagc cttcgctggc gcgagcatac cgatatacgg aaacggaggt
     7741 gcaaccacga tttcatgagt tccctttgcg gcaagacgct tcgcgccctg atacagcgtt
     7801 tttgcctttg cacgggtctc gacgtacgcc ttccagtttc cgacgatgag catggctcaa
     7861 tcgtagcacg tggcatctat tcctcccgcc agtctacgaa ctggtaatcc gtggatgtct
     7921 gcggggtaaa cggcggcgga tcagttgcaa gcttgcgatc gaaagagtcg acacggctct
     7981 ggtaccccgc atccgaatat cccccgaaca aggaccagca tccgttctgc cactgcgtac
     8041 ccgtacgcat attggagaca gtcgtaccga ggatggtgag cgaggtacgc ggttcgtacg
     8101 aagacgggca ggcataccgg ttgcgcccaa aggaaccacc ctgcgccacg aagataccgt
     8161 tcagtgtcat gttctgcgga gagttcggag ttatgaggat gttgttctcc gagataaccg
     8221 taaggccgtc agatccgtcg gtcgctgcgt accgcaggtt tccaggaagc atcacgttcg
     8281 ttttcacgtt ggttagggtc ggggttgccg agaccacggt caccttctga gagacgatgc
     8341 cttcgatcca gacatggtct tcaacgaaga taagagcgca atcgctcgga agcgtaagcg
     8401 tctggta
//
LOCUS       DASWXU010000041         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_748220, whole genome shotgun sequence.
ACCESSION   DASWXU010000041 DASWXU010000000
VERSION     DASWXU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748220"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..458
                     /locus_tag="VGE48_01425"
     CDS             99..458
                     /locus_tag="VGE48_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042063.1"
                     /translation="MRLFWLLAALVLASLLAYFQYVALEHYLYWRYLWLDTFMHFLGG
                     LTVSTFLIALLDKHRAIVFLSGMFAIAVGWELFELGINAEREANFGFDTSLDLLMDAV
                     GMSLGYLIARYTLWRSA"
     gene            443..1831
                     /locus_tag="VGE48_01430"
     CDS             443..1831
                     /locus_tag="VGE48_01430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1042064.1"
                     /translation="MALRVTFHGGAGAVTGSNFLVEGTNGGKILIDCGLEQGKDFCVP
                     CMYAPFPYDTTKIDALVITHAHLDHIGRAPKLVREGFRGKAYMTEATRDLMALMLTDS
                     VGLLAQEAKRYGQTPLYGEEDVTRLLSLVEILPYHEEREIAPGLSVYLRDTGHILGAG
                     SVRVKDADGTTAAFTGDIGNSPNPYLRDAEPVPDADALIMEAVYGDRVHEHVEERVPA
                     LRDALKRALTRGGTMLMPAFSMERTQLMLYELSNLMEAGELPKFPVFLDSPLAIGVTD
                     IYEQHAAEYFNDAAKAETKKETSIFRFQFLTMTQSREDSETIAGEPDPKLIIAGAGMS
                     HGGRIGKWERKYLPDPDTTLFIVGYQAPGSPGRLLQDGAKKVRIDGQEVKVRAKIESL
                     TSWSAHADREGLLRFAKGSLPRAKAIFVSIGEPSAQRFLAQRIHDFLGAKAVVPSQGE
                     VWEITKEGVKKV"
     gene            complement(1877..2227)
                     /gene="rplT"
                     /locus_tag="VGE48_01435"
     CDS             complement(1877..2227)
                     /gene="rplT"
                     /locus_tag="VGE48_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY1042065.1"
                     /translation="MARVKGGVIAAKKRRSILKRAKGYRHGRSTKVRLAKESLVHAGK
                     NAFNHRRDKKTDFRQLWIVRLNAALRDLGFKSYSTFIAAKAKAGIELDRKVLATLAAT
                     EPEVFTRVAKKIQG"
BASE COUNT          458 a          610 c          703 g          483 t
ORIGIN      
        1 gcgggcaccc acgtggcatc caaaagttgc cactagggac ggttcttccg agaaacccga
       61 gacgaaggtc tcgggttttc gcgtgctaga gtgtagccat gcgccttttc tggctccttg
      121 ctgcgctcgt gctcgcttca cttctcgcgt acttccagta cgtcgcgctc gagcattacc
      181 tgtactggcg gtatctatgg ctcgatacat tcatgcattt tctgggaggc cttacggtct
      241 cgaccttcct tatcgcactc ttggacaagc atcgcgcgat tgtattcctc tcaggaatgt
      301 tcgctattgc ggtggggtgg gagctgtttg agctcggcat caacgctgaa cgggaggcga
      361 atttcggctt tgatacctcg cttgatctct tgatggatgc ggttggtatg tcgcttggat
      421 atttgatagc tcgctacaca ctatggcgct ccgcgtaacc tttcacggcg gcgccggtgc
      481 cgtcaccggc tctaacttct tggttgaagg aaccaatgga gggaagattc tcatagactg
      541 cgggctcgag caggggaagg acttctgtgt tccgtgcatg tacgcgccgt tcccgtacga
      601 caccacaaag atcgatgcgc tcgtcattac gcacgcacat ctcgatcata ttgggcgcgc
      661 tccgaagctc gtgcgtgaag gattccgcgg caaagcatac atgacggaag cgacacgcga
      721 ccttatggcg ctcatgctga ccgatagtgt gggactgctc gcgcaggaag cgaagcgcta
      781 cggacagacg ccgctctacg gagaagagga cgtgacacgg cttctttcgc tcgtcgagat
      841 ccttccgtat cacgaggagc gcgagatagc gccgggactt tccgtgtacc tgcgcgatac
      901 cggacatatc ttgggagccg ggagcgtgcg ggtgaaggac gcggacggca ctacggctgc
      961 gttcacgggc gatataggga attcgccgaa cccgtacctg cgcgatgcgg agccggtacc
     1021 tgacgcggat gcgctcatca tggaggcggt atacggagac cgggtgcacg agcacgtgga
     1081 ggagcgtgtg cctgcgctcc gggacgcgct taagcgagca cttactcgcg gcggcaccat
     1141 gctcatgccg gccttctcca tggagcgcac gcagctcatg ctgtacgagc tctcgaatct
     1201 catggaggca ggagaactcc cgaagttccc agtattcctc gattcgccgc ttgcgatcgg
     1261 ggttacggat atctacgagc agcatgcggc agagtatttc aatgacgccg ccaaggcgga
     1321 gaccaagaag gagacgagca tcttccggtt ccagtttttg accatgacgc agagcaggga
     1381 ggactcagag actatcgcag gcgagcctga cccgaaactc atcatcgctg gagccggcat
     1441 gtctcatggc ggccgcatcg ggaaatggga gcggaagtat cttccggacc ccgacaccac
     1501 gctgtttatc gtgggatacc aggcaccagg ctctccggga cgtctgcttc aggacggggc
     1561 gaagaaggta cgaatcgacg gacaggaggt gaaggtgcgc gcgaagatcg agagccttac
     1621 gagctggtct gcgcacgcgg atagggaagg acttctccgg ttcgcgaagg gttcgcttcc
     1681 gcgcgcaaaa gcaatcttcg tgtctattgg cgagccgagc gcacaaagat tcctcgcaca
     1741 gcgtatccat gacttcctcg gggcgaaggc ggtggtgccg agccagggag aggtgtggga
     1801 gataacgaag gagggagtga agaaggtata agaaaagccc cgctaagtgc ggggcttttc
     1861 ttatgaagga ggggtcttaa ccctggatct tctttgcgac gcgcgtaaat acctcaggct
     1921 cggttgcggc aagcgttgcg agaaccttgc gatcaagctc gatgcctgcc tttgccttag
     1981 cagcgatgaa ggtgctgtag gacttgaagc cgaggtcgcg gagcgctgcg ttaagacgca
     2041 cgatccagag ctgacggaag tcggtcttct tgtcgcggcg gtggttgaac gcgttcttac
     2101 ctgcgtgtac cagcgactcc ttcgcgaggc gaaccttcgt ggagcggccg tggcggtagc
     2161 ccttcgcgcg cttcaaaatg ctgcgacgct tctttgctgc aattacacca ccctttactc
     2221 gtgccatacg gaattagtta ctgaaagtta tgcg
//
LOCUS       DASWXU010000042         8076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_448678, whole genome shotgun sequence.
ACCESSION   DASWXU010000042 DASWXU010000000
VERSION     DASWXU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448678"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="VGE48_01440"
     CDS             complement(<1..1748)
                     /locus_tag="VGE48_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1042066.1"
                     /translation="MARERFDHQEIEKTQQARWQELDLYRADLTDTERDPYYLLFEFP
                     YPSGDLHIGHWYAFALTDIFARMLRMQGKNVLFPMGFDSFGLPAENAAIKYGKDPAAW
                     TNANMETMRTQVRSMGTSVDWSKEVVTSSPEYYKWTQWLFTKLYDHGLAYRKNAPVKW
                     CPKDQTVLANEQVNDGKCERCGTEVEEKNLTQWFMGITKYADRLITDLDPLPWREDIK
                     DAQRAWIGKSEGARIPFRLVFGNEEKDAWRDQDGNPAQVSVFTTRPDTIFGATYLVLA
                     PEHPWVRAIWDTPHDDAILNKDEVRAYVEASQKKTERERQENKEKTGVRLEGISAINP
                     ATQEEIPVFVADYVLASYGTGAVMAVPAHDERDFEFAKRMELPIKRVIDPVTGEEQES
                     PAHKEKIVAVVEHEGKVLTINWKPELGGRLLVGGTVEKNEDPAVTAAREITEETGYID
                     LEFIERADEKVHHGYFAFSKNQATLAHTTLLHFRLKSDACDAQKLEDNEKDKFEVEWV
                     SVEQAEREIHDDQHLYTLARFMRPSAYKGDGLLRNSGQFDARDNREAIDDIIAFVGGE
                     KVTNYRLRDWLVSRQR"
     gene            complement(1750..2703)
                     /locus_tag="VGE48_01445"
     CDS             complement(1750..2703)
                     /locus_tag="VGE48_01445"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEY1042067.1"
                     /translation="MEQAPTRTTPRDFFLWAGAVIALYGSVLSLITLLFEYVNHAFPD
                     PLAYWGDPYGGAVRAAMAGVIVLVPTTIILLRIIRGSITKEPGKANIWVRRWALVLTV
                     FIAIAVILIDLITLITTFLGGELSVRFGLKVAIVLLVALGVFLHFLADLKGYWAAHPK
                     KADLVGIGAGILALVAVVAGFFVIGTPSTIRELRYDEQRVSDLQSIQYQIVNFWQLKE
                     ALPADIAELNDPLSGFMVPVDPDSGAPYRYVRTSDSSFELCATFSQETPETKGQGSFP
                     RGDVSYPSMMPNENWQHGAGEVCFSRTIDPERYPPFGKPVR"
     gene            complement(2743..3333)
                     /locus_tag="VGE48_01450"
     CDS             complement(2743..3333)
                     /locus_tag="VGE48_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1042068.1"
                     /translation="MTSGATRKIEIGCGRKPREGYESCDIRAIPGVDHICEAGKLPFE
                     DGTIDEIYTRHLIEHFTLKEFIEVLAEWNRVLATGGVLYVITPNLLWHAKQLLEGSHE
                     SFYTTQRGQNDRYWAFGSIFGWQQDEHDIHKFGYYYELLKDILHEAGFGDVENRTNTP
                     ESIEQAEHHLEVRALKTSSFTEPHNSRFFSHLNVTH"
     gene            complement(3343..4437)
                     /gene="ychF"
                     /locus_tag="VGE48_01455"
     CDS             complement(3343..4437)
                     /gene="ychF"
                     /locus_tag="VGE48_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY1042069.1"
                     /translation="MKIGIVGLPNVGKSTLFNALTKAAVPAENYPFCTIDPSVGVVGV
                     PDTRLDQLAALSKSEKVVPAAVEFVDIAGLVKGASEGEGLGNQFLSHIREVDAILQMV
                     RFFDDPDVTHVHGDVDPARDIEIINLELILADQQSVLSRREKLVRDVKMNNKDALAED
                     AVLAQIDQALKEGKLARSVSLSEEDAKRIKSLNLLTMKPILYSFNGKAGGHNLSELND
                     GRAQAAYDLVESMGSRYVEVDARTEHELNDVADEDKDMFRSELGVQVDGLDALIRAAY
                     DTLGLITFFTTGPKESRAWTIKRGALAPDAGAAIHNDFKDKFIRAEVVAWDTLLQDGS
                     WSAARDKGDIKTEGKTYEVKDGDVMVFLHS"
     gene            4540..4758
                     /locus_tag="VGE48_01460"
     CDS             4540..4758
                     /locus_tag="VGE48_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042070.1"
                     /translation="MFKVDTKFFFISLVGIALALIFWPFAGMHIFTTFNWWQIPVFWA
                     VINLPGHIGHHILSNLWGRFELKMEARK"
     gene            complement(4952..5536)
                     /locus_tag="VGE48_01465"
     CDS             complement(4952..5536)
                     /locus_tag="VGE48_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY1042071.1"
                     /translation="MRSKRIFILLGHPDQGPEPMSRQLADCYEEAAKVAGHEVRRMNL
                     FDLSFDPTLHKGYRAIQELEPDLVKVQENLSWCEHFVLAYPNWWGGMPAPLKGMWDRM
                     FLPRFAFRMWKNRFGWDALLKGRTARVLITCGNPAILDHLAFGDFTASIKRSLLQFAG
                     FRTWVSAFGHAEHASPAKQERWKRTVSHLAKRAR"
     gene            complement(5539..5769)
                     /locus_tag="VGE48_01470"
     CDS             complement(5539..5769)
                     /locus_tag="VGE48_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042072.1"
                     /translation="MALQDFLLKQAMKWKMKDVPKEQQEQIMALVQKDPELFKRIGEE
                     VEKRVKGGEPQMKATMEVMKKYRTELAALVQK"
     gene            complement(6359..6607)
                     /locus_tag="VGE48_01475"
     CDS             complement(6359..6607)
                     /locus_tag="VGE48_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY1042073.1"
                     /translation="MAKTFHLTIANVGENLFSGEAVSVTLPGVEGVFTVMAGHEAFVT
                     PLQEGIALVKTADGAETSYTLHRGGIAEVSGGQATVLL"
     gene            complement(6607..7983)
                     /gene="atpD"
                     /locus_tag="VGE48_01480"
     CDS             complement(6607..7983)
                     /gene="atpD"
                     /locus_tag="VGE48_01480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY1042074.1"
                     /translation="MNTGTITEIIGPVVDVHFEKERPAIQDALVVDGNDTHGKIVLEV
                     AAHLGLDRVRCISMNETAGLSRGEKATATGAPISVPVGQAALGRLFNVLGEAIDGKAE
                     VPASTERLPIHRMPPTFDEQTTKAEIFETGIKAIDLMIPFIKGGKVGLFGGAGVGKTV
                     TIQELIRNVAQEHGGYSVFAGVGERVREGNDLYHEMEESGVLDKTALVFGQMNEVPGA
                     RARVALTGLTQAEYFRDEGGKDVLFFMDNVFRFIQAGSEVSSLLGRVPSAVGYQPTLA
                     EEMGKLQERITSTKKGSITSIQAVYVPADDLTDPAPATTFAHLDGTVVLSRQLAALGI
                     YPAIDPLASSSTALTPEIVGEEHYKVANQVKQVLQRYKDLQDIIAILGIEELSDEDRL
                     TVARARKLERFLSQPFFVGEVFTGNPGQYVTREETVRGFKEIVEGKHDDKPESAFYMK
                     GGIDQVTG"
BASE COUNT         1748 a         2388 c         2091 g         1849 t
ORIGIN      
        1 tatcgctggc gcgatacaag ccagtcacga agacggtagt tcgtgacctt ttcgccgcca
       61 acaaacgcga tgatgtcatc gattgcctcg cggttgtcgc gagcatcgaa ctgaccggaa
      121 tttctaagaa gcccgtctcc cttatacgca cttgggcgca tgaagcgcgc aagtgtatag
      181 agatgctgat catcatgaat ctcgcgctcg gcctgctcga ccgacaccca ctcaacctca
      241 aacttatctt tctcgttatc ttcgagcttc tgcgcatcgc aggcatcact ctttaatcgg
      301 aaatgcagga gtgtcgtgtg ggcgagtgtt gcctgattct ttgagaaagc gaagtatccg
      361 tgatgtacct tctcatctgc ccgttcgatg aattcaagat cgatataccc tgtctcctct
      421 gtgatctcac gggctgcggt caccgctgga tcctcatttt tttctacagt accaccaacg
      481 agaagacgac cgccaagctc tggcttccaa ttaattgtaa gcaccttacc ttcgtgctct
      541 accacggcaa caatcttctc cttatgggca ggagactcct gctcctctcc ggtcactgga
      601 tcaataactc gctttatcgg aagctccatc ctcttcgcaa actcaaaatc gcgctcgtca
      661 tgcgccggta ctgccataac cgcaccggtg ccgtatgacg cgagcacgta atcagcaaca
      721 aagaccggga tctcttcctg tgtcgcagga ttaatagcgc tgattccctc aagtcgtacg
      781 cccgtcttct ctttattctc ctggcgttcg cgctcagtct tcttctgcga ggcttctacg
      841 tatgcacgaa cctcatcttt attcaaaatg gcatcgtcat gcggggtatc ccagattgca
      901 cgcacccacg ggtgctcggg agcaagcacg agataggtcg caccgaagat ggtgtctggg
      961 cgcgtggtga atacggagac ttgcgccggg ttcccgtctt gatcgcgcca cgcatccttc
     1021 tcctcgttcc cgaacacaag gcggaaaggg atgcgtgcgc cctcagattt tcctatccac
     1081 gcgcgctgcg catccttgat atcttcgcgc cacggaagcg gatcgaggtc cgttatgaga
     1141 cggtccgcgt acttcgtgat gcccatgaac cactgcgtaa ggttcttctc ctcgacttcc
     1201 gtgccgcagc gctcgcactt tccatcgttc acctgctcgt tggcgagcac ggtctggtcc
     1261 ttaggacacc acttcaccgg tgcgttcttc cggtacgcga gcccatgatc atatagcttc
     1321 gtaaacaacc actgcgtcca cttgtagtac tcgggactcg aggtaactac ttccttcgac
     1381 cagtccacgg acgttcccat ggagcgcacc tgtgtgcgca tggtttccat atttgcgttt
     1441 gtccacgctg cgggatcctt cccgtactta atagcggcgt tctccgcagg aagcccgaag
     1501 gaatcgaaac ccatggggaa taggacgttc ttcccctgca tgcgcagcat gcgggcaaag
     1561 atgtctgtga gagcaaaggc gtaccagtgt cctatatgga ggtctcccga agggtacggg
     1621 aactcgaaca ggaggtagta cggatctcgt tcagtatccg taaggtccgc gcgataaaga
     1681 tcgagctcct gccagcgcgc ttgctgcgtc ttctctatct cctgatgatc aaatcgctcg
     1741 cgcgccatgc tagcggaccg gctttccgaa cggcgggtag cgctcagggt cgatggtgcg
     1801 cgagaaacac acctcgcctg ccccgtgctg ccagttctcg tttggcatca tgcttgggta
     1861 cgatacgtcc cctcgcggga atgaaccctg acccttcgtt tctggagtct cctgactaaa
     1921 ggtggcacag agctcgaacg agctgtcgct cgtacggacg tagcgatacg gcgcgccaga
     1981 atccggatct accggcacca tgaagcctga gagcgggtca ttgagctcgg cgatatccgc
     2041 cggaagcgct tccttaagct gccagaagtt cacgatctga tactgaatgg actggagatc
     2101 cgagaccctc tgctcgtcgt agcggagctc gcggatggta cttggtgtgc cgataacgaa
     2161 gaatccggcc accactgcaa cgagcgcgag gatgcccgcc ccgataccta caagatcagc
     2221 tttctttgga tgcgctgccc aatatccctt gaggtctgca aggaagtgga ggaacacgcc
     2281 gagcgctacc aggagtacga tcgctacctt gaggccaaag cgcacggaga gctcgccgcc
     2341 gaggaaggtg gtgatgagcg tgatgagatc aatgaggata accgcaatcg caatgaacac
     2401 cgtgagcacg agcgcccagc ggcgtaccca gatgttcgcc ttgcctggtt ccttcgtaat
     2461 cgatccacgg atgattcgaa gcaggatgat cgtagtcggg acaagcacta tgacgcctgc
     2521 catagcggca cgtaccgcgc caccgtacgg atcaccccag taggcgagcg gatcaggaaa
     2581 cgcgtggttc acgtactcga aaaggagcgt gatgagcgag aggaccgaac cgtagagtgc
     2641 aatgactgcg ccagcccaaa ggaagaaatc acgaggagtc gtacgtgtag gtgcctgttc
     2701 catagtgcgc ggattatacc acaaaagact atatgtgacc cgttaatgcg taacgttcaa
     2761 atggctgaaa aatctcgagt tgtgtggctc tgtaaacgaa cttgtcttaa gtgcccgcac
     2821 ttcgagatga tgctccgcct gctcaatact ttctggggta ttggtgcgat tttcgacatc
     2881 accgaaaccg gcctcatgca gaatatcttt tagtagttcg tagtaatacc cgaacttatg
     2941 gatatcgtgt tcgtcctgct gccacccgaa gatacttcca aatgcccagt agcgatcatt
     3001 ctgacctcgc tgcgttgtgt aaaaactctc atgacttcct tcgagtagtt gctttgcatg
     3061 ccacagtagg ttcggagtaa tgacatacaa caccccgcca gtagctagca cacgattcca
     3121 ttcagcgagg acttcaatga actccttgag cgtaaaatgc tcgattagat gccgagtata
     3181 gatttcatct attgtgccat cctcgaatgg cagcttccct gcttcacaaa tgtgatcgac
     3241 gccaggaatg gctcgtatat cgcatgattc atagccctcc ctgggtttcc tgccgcagcc
     3301 tatttctatc ttccgtgtcg ccccagaggt catgatcctt ggttaactat gcaggaacac
     3361 catcacgtcc ccgtccttca cctcgtacgt cttcccttcg gtctttatgt cacccttgtc
     3421 acgcgcggcg ctccaggagc cgtcctgtag aagcgtgtcc cacgctacaa cctccgctct
     3481 aataaacttg tccttgaaat cgttatgaat agccgctccc gcgtcaggcg cgagcgcacc
     3541 gcgctttatg gtccaggcac gggattcctt agggccggtt gtgaagaagg tgatgaggcc
     3601 gagggtgtcg tatgcagcac ggataagcgc atcgagtccg tccacctgca ccccaagctc
     3661 cgagcggaac atgtctttat cctcgtccgc tacgtcgttc agctcgtgct cggttcgcgc
     3721 atccacctca acgtaacggc tacccatgga ttcaacgaga tcgtatgcag cctgcgcacg
     3781 accgtcattc agttctgaaa gattgtgccc tcccgccttt ccgttaaagg aatagagaat
     3841 cggcttcatc gtaagcagat tcagggactt gatgcgcttc gcatcctcct cggatagcga
     3901 tactgagcgc gcaagctttc cttccttaag cgcctggtcg atctgcgcaa gaaccgcgtc
     3961 ttctgcgagc gcgtccttgt tattcatctt cacgtcgcgc accaatttct cgcgacggct
     4021 caatacggac tgctggtccg caaggatgag ctcgaggttg atgatctcta tatcgcgcgc
     4081 aggatcgaca tccccatgca catgcgtcac gtccggatcg tcgaagaagc gcaccatctg
     4141 aaggatcgca tccacctcgc ggatgtgtga gaggaactgg ttaccaaggc cctcgccctc
     4201 gcttgccccc ttcacgagcc ccgcgatgtc cacgaactca acggcagccg ggactacctt
     4261 ctcgctcttt gagagcgctg cgagctgatc aagccgagta tcaggaacgc caaccacacc
     4321 taccgaggga tcgatagtgc aaaagggata gttttcagcg ggaaccgccg ctttggtgag
     4381 tgcattaaag agagtggact ttccaacatt cgggagccct acgatgccga ttttcatatg
     4441 cgtacagtaa cgggaacatg cgtttaaggc aaaggtatag cttgactcta cctttaaacg
     4501 ctggtattac taacccgact ttgaagaaag gagatttcaa tgttcaaggt cgacactaag
     4561 ttctttttca taagcctggt aggcatagcg ctcgcgctca tattctggcc cttcgccgga
     4621 atgcatatat tcacgacgtt taactggtgg cagatccctg tattctgggc agtcatcaac
     4681 ctacccggcc atataggcca ccatattctc tcgaaccttt ggggacggtt cgaactgaaa
     4741 atggaggcca ggaagtaatg aacttctcag agcctcgtct aaagcacgca cgtcagtacc
     4801 gcccgctttt ccggggctct gatcatttat atacgcaaaa ccgcctcaag aagaggcggc
     4861 ggtaccgggg cacccgcgac gggcgccaag tcgatttgcg taatcaggaa ccaagcgttg
     4921 atgcaggtgc cccggcccca aaagcatgcg cttatcgtgc tctctttgca agatgcgaaa
     4981 ccgttcgctt ccagcgttcc tgctttgcag gtgacgcatg ctcagcgtgc ccgaacgcag
     5041 atacccaggt acggaaccct gcaaactgaa gcagagatcg cttgatagaa gcagtgaagt
     5101 ctccgaacgc gagatgatcc aggatcgccg ggttgccgca ggtaatcaga acgcgtgcgg
     5161 tgcgaccctt caaaagcgca tcccacccaa agcgattctt ccacatacga aacgcgaagc
     5221 gaggaaggaa catgcgatcc cacataccct tcaggggcgc cggcattcct ccccaccagt
     5281 tcggatacgc gagcacgaaa tgctcgcacc atgagaggtt ctcctgaacc ttcacgagat
     5341 ccggctcgag ctcctgaatt gcacggtatc ccttatgaag tgtcggatcg aaggagagat
     5401 caaaaagatt catgcggcgc acctcgtgtc ctgccacttt cgctgcttcc tcgtagcagt
     5461 ccgcgagctg tcggctcatg ggttcaggac cctggtccgg gtgcccgaga aggatgaata
     5521 tgcgcttact ccgcatggct atttctgcac gagcgccgcg agctcggtgc ggtacttctt
     5581 cataacctcc atcgtagcct tcatctgcgg ctcgccgcct tttacgcgct tctcgacttc
     5641 ctcaccgata cgcttgaaga gttccgggtc cttctgcacg agcgccatga tttgctcctg
     5701 ctgttccttc ggaacgtctt tcatcttcca tttcatagcc tgcttaagca ggaagtcctg
     5761 tagtgccata tgagaagagt atagcaaaaa ggcccgcgct tcacagtacg gaccttcgat
     5821 ttgcaactcg catcctcctt aaataccgac gtctagctcc aacgaatcta atcgtctttg
     5881 cccgtacatc cacatcgatc atttctgggt gcaaaatgag cacaacacac tcgctctttg
     5941 gcctttgctc ccaaaacttc tccatgcctc gcattgaaga cttaatctgc aaggggattt
     6001 cgccgacatc ggtgatagca aacgcatcaa tgccgttctg gtcatccgac ctggttccct
     6061 tccggattcc gtagaaccaa tcgggaagag aggtactcgg ttgattgaaa gtggagaaca
     6121 ggcgaccttc agtttgtaag ccacgaagat gagcgagctt tcgcaccctc ttctcttcag
     6181 catgaacttc ttcaaatgtc ctttccgaag attgattctc cttttcccaa tctctacgcc
     6241 tcttgcgaag ccggagtgcc cttctccttt cggtgtttct atttcccaac taccgcctcc
     6301 ttgatggctg ttccaaggag actctacagg ggtgctaaca acaccgcaag caagtgtatt
     6361 agagcagcac cgtagcctgc cctccggaca cctcagcaat gccgccccta tggagggtgt
     6421 acgaagtctc agctccgtcc gcggtcttca caagcgcaat accttcctga agcggcgtaa
     6481 caaatgcctc atgtcctgcc atcaccgtga agacgccttc gacgcccgga agcgtcacgg
     6541 agaccgcctc tcctgagaag aggttctcgc cgacgttcgc gatagtgaga tggaaggtct
     6601 ttgccattat ccggtgacct gatcgatacc gcccttcatg tagaaggcgc tctcaggctt
     6661 gtcgtcatgc tttccttcta caatctcctt gaagccgcgt accgtttcct cacgggtaac
     6721 gtactggcct gggtttccgg tgaagacctc gcccacgaag aacggctgcg agaggaagcg
     6781 ctcgagctta cgcgcacgag caaccgtaag acgatcctca tctgaaagct cctcgatacc
     6841 aaggatggca ataatgtcct ggaggtcctt gtagcgctgc agcacctgct tcacctggtt
     6901 ggcaaccttg tagtgctcct cgcccacgat ctccggcgta agtgcggtcg agctcgatgc
     6961 aagcgggtcg atagctgggt agataccgag cgcagcaagc tggcgggaaa gcaccacggt
     7021 accgtcaagg tgcgcgaagg tggtagccgg agccgggtcc gtaaggtcgt cggccggaac
     7081 gtacactgcc tggatggacg tgatggagcc cttcttcgtc gacgtaatac gctcctggag
     7141 cttacccatc tcttcagcaa gcgtcggctg gtatcccacg gccgaaggca ctcgaccgag
     7201 gagcgaggac acctcggaac ctgcctgaat gaagcggaac acgttgtcca tgaagaagag
     7261 gacgtccttt ccgccttcgt cacggaagta ctcagcctgc gtaagaccgg tgagcgcaac
     7321 gcgcgcacgg gcgcccggca cctcgttcat ctggccgaac acgagcgcgg tcttatcgag
     7381 cacgcctgac tcttccatct cgtgatagag gtcgttaccc tcgcgcacac gctcgcccac
     7441 gcccgcgaac acggagtagc cgccgtgctc ctgcgccaca ttgcggatga gctcctgaat
     7501 ggtcaccgtc ttgcccacgc ccgcgccgcc gaagagaccc acctttcctc ccttgatgaa
     7561 cgggatcatg aggtcgatcg ccttgatacc ggtctcgaag atctcggcct tcgtagtttg
     7621 ctcgtcgaac gtcggcggca tgcggtggat cgggaggcgc tcggtagaag ccggaacctc
     7681 agctttaccg tcgatagcct cgccaagcac gttgaagagg cgaccaagcg cagcctggcc
     7741 cacaggaacg gagatcggtg caccggtagc agtcgccttc tcgccgcgcg agaggcctgc
     7801 ggtctcgttc atcgagatgc agcgcacacg atcgaggccg aggtgtgccg cgacctcgag
     7861 cacgatcttg ccgtgcgtat cgtttccgtc tacgacgagt gcgtcctgaa tagccggacg
     7921 ttccttctcg aagtgcacgt ctacgactgg cccgatgatc tctgtgatgg ttcctgtgtt
     7981 cataatgatt tggttataaa ttcgcccagt acctaaactg ataacgcctc gcggccgccc
     8041 acgatttccg agacctcgcg ggtaatcgcg gcctga
//
LOCUS       DASWXU010000043         4553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_200739, whole genome shotgun sequence.
ACCESSION   DASWXU010000043 DASWXU010000000
VERSION     DASWXU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200739"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VGE48_01485"
     CDS             complement(<1..537)
                     /locus_tag="VGE48_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042075.1"
                     /translation="MALSDTRILEEMKKGDIVIHPFNDDQLATSSYDVTLGEYFYREQ
                     PPKYNHSLYNIWSEEHTRHVWGADDVQRAVTAKEAFKKYNFEWDGIRPDDKVIIMRPG
                     ETILAHTQEFIGGKDHITTMMKARSSTGRQFIEVCKCAGWGDVGYVNRWTMEITNNSK
                     NYIIPLVVGRRIAQIIFFE"
     gene            complement(610..1980)
                     /locus_tag="VGE48_01490"
     CDS             complement(610..1980)
                     /locus_tag="VGE48_01490"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY1042076.1"
                     /translation="MENTMRTLSDLLHHSVPLGDGGEVLVLNTGAIIDAEAAAMLGAL
                     HSRSAGGIRGHLKVLEEKGASNFMANFYVGYGHKSIGDLGDAHIFIEGVSMLAAKAIQ
                     DFPLYNGQEVSTRYVDFSQQRFVDPAGTPESAALLEDLRAFYLAGIEELVRTLPERFP
                     RGPEESEKKYEKAIKARAFDIMRSFLPAGASTNLVWVGPLRQFADRLPTLRNHPLPEV
                     RDLAAALEQSLLSAFPNSFSDKRYEATEAYHELCGSKYAYFDDAGCPEFALTHDALRP
                     ERLADYRDALSERPPKTELPFAARECGTLEFQFLLDFGSYRDLQRHRAVIAPMPLLTG
                     AHGFEPWYLEELPEGLRAKAQSFLTEYEERVAALGLSPELAQYYLPMGFRTANRLTGD
                     LRALVYLVELRAGSTVHATLRRRAIEMARVLEERCTEYGLALHYDQEPDRFDVKRGDQ
                     DIVLKD"
     gene            2126..2830
                     /locus_tag="VGE48_01495"
     CDS             2126..2830
                     /locus_tag="VGE48_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY1042077.1"
                     /translation="MTHAAKGKIIVLDGNDGSGKETQSKLLAERFRKEGLEVYLESFP
                     RYKKSKAGKFIGELLNGEHGSISTLSPELMSVPYALDRVGSSAEILQAVSEGRVVILD
                     RFTSSNQMHQGGKIEDEQERIAFLKWLDELEHEELGIPRSDCVVYLKVPVDISVKLLK
                     EKRAAKNGSLKEGELDQVESDRLYLERSHAMANWLAQREQSWSVIDCTNESGDMRSRE
                     EIAEDIFSSVVRQLEA"
     gene            2966..4267
                     /gene="glyA"
                     /locus_tag="VGE48_01500"
     CDS             2966..4267
                     /gene="glyA"
                     /locus_tag="VGE48_01500"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY1042078.1"
                     /translation="MSQYDHIQNEDQDVFRAIVGEETREAEGLELIPSENYVSTAVRE
                     AMGSVLTNKYSEGYPGKRYYGGQEFTDQVETLAIERAKSLFRADHANVQPLGGAAANI
                     AAYFAWLEPGDTILGMDLAHGGHLTHGSPVTYMAKLYNFVRYGMKDPDTGEIDYDEML
                     RVAKETRPKIVLAGFSGYPRTIDWKRVADIAKEVDAVAMADVAHIAGLIAGGALENPL
                     DHGFHIMTTTTHKTLRGPRGAMILSKGTVSSPLKAPPKAVENLPTLIDRSVFPGFQGG
                     PHMHQIAAKAIAFKEAATPAFREHAAQVIKNAQAMAGVFMDRGVRLIGGGTDNHLILA
                     DVYGSLGVSGGEAETVLDAAGITLNKNSIANDTRKPFDPSGIRFGTPAMTTRGFTEED
                     SKEVANIMLDVLTKRDEETVSAARTRVREIALAHPVPESFI"
BASE COUNT          969 a         1278 c         1408 g          898 t
ORIGIN      
        1 ctcgaagaag atgatctgcg cgatgcggcg tcccaccacg agcgggatga tgtagttctt
       61 cgagttgttc gtgatctcca tggtccagcg gttcacgtag ccgacgtcgc cccagcctgc
      121 gcacttacag acctcgatga actggcggcc ggtagaggag cgggccttca tcatggtggt
      181 gatgtggtcc ttgccgccga tgaactcctg cgtatgcgcg aggatggtct cgcccgggcg
      241 catgatgatc accttgtcgt caggacggat gccgtcccac tcgaagttgt acttcttaaa
      301 ggcttccttt gcggttacgg cgcgctgcac atcgtctgcg ccccagacat ggcgggtatg
      361 ctcctcgctc cagatgttgt agagagaatg gttgtacttt ggtggctgct cgcgatagaa
      421 gtactcgccg agggtcacgt cgtagcttga ggtggcgagc tggtcgtcat tgaaaggatg
      481 aataacgatg tcgcccttct tcatttcttc gaggatacgg gtgtctgaaa gtgccatagt
      541 gccccgatta tacgggtagg gagacataaa aggaaaagtg ccccggatcc tgagatccgg
      601 ggcacccact tagtccttga gcacgatgtc ctgatcgccg cgcttcacgt caaagcgatc
      661 cggctcctgg tcgtagtgga gggccaggcc gtactcggtg cagcgttcct caagcacccg
      721 tgccatctca atcgcacggc gccggagcgt cgcgtgcacg gtgcttccgg cgcgcagctc
      781 cacgagatag acgagggccc gcaggtctcc ggtaagccga ttggcggtcc ggaatcccat
      841 gggaaggtag tactgcgcaa gctccggaga gagcccgagt gcggcaaccc gctcctcgta
      901 ctcggtgagg aacgattgcg ctttcgcgcg aagtccctca ggaagctcct ctagatacca
      961 cggctcgaac ccgtgcgcgc ccgtaagaag cggcatgggc gcgattaccg cgcggtgtcg
     1021 ctgcaggtcg cgatacgagc cgaagtccag caggaactgg aactcgagcg tcccgcattc
     1081 gcgagcggcg aacggcagct ctgtctttgg aggacgttcg gagagcgcgt cgcgatagtc
     1141 tgcgaggcgt tctggccgaa gcgcgtcatg cgtgagggcg aactccgggc agcccgcgtc
     1201 gtcgaagtag gcgtacttag agccgcagag ctcgtgatac gcctcggtcg cttcgtaacg
     1261 cttatccgaa aacgagttcg gaaaggcgct cagaagtgac tgttcaagcg cagccgcgag
     1321 atctcgcacc tccggaagcg gatgattgcg aagggtggga agcctgtccg cgaactggcg
     1381 aagcgggcca acccacacga ggttcgtact cgcgccagcg ggcaggaacg agcgcatgat
     1441 gtcgaacgcg cgggccttga tagccttctc gtacttcttc tcgctctctt ccggtccgcg
     1501 cgggaagcgc tccggaagcg tgcgtaccag ctcctcgatg cctgcgagat agaacgcgcg
     1561 caggtcttca agaagcgctg cggattccgg cgtgcccgcc gggtccacga agcgctgctg
     1621 cgagaagtcg acgtagcggg tcgacacctc ctgcccgttg tagagcggga agtcctggat
     1681 cgccttggcg gcgagcatcg agacgccttc gatgaagatg tgcgcgtctc cgaggtcgcc
     1741 gatcgacttg tgtccgtagc caacatagaa gttggccatg aagttcgacg cgcccttctc
     1801 ttcgaggacc ttgaggtgtc cgcgaatgcc gccagcagag cggctatgga gcgcgccgag
     1861 catcgctgcc gcttccgcgt cgatgatcgc tccggtgttg aggaccagca cttctccgcc
     1921 gtcaccgagc ggaacgctgt ggtgtaaaag atcagacagg gttcgcatgg tgttctccat
     1981 ataatccttg agagtctacc acgcagtcta cgggccctgc ggaggcccgg caaatgagga
     2041 tttttccttg taatgcttgg ggatagggaa gaagtcaagc gtgctacggt tacctgagat
     2101 tattgttcac agggataccg aaatcatgac tcacgccgca aaaggaaaaa tcatcgtcct
     2161 cgacgggaac gatggctccg gcaaggaaac acaatccaag ctccttgccg agcgcttcag
     2221 aaaggagggt ctcgaggtct acctggagag cttcccgcgt tacaagaaaa gcaaagccgg
     2281 aaaattcatc ggcgagcttc tcaatggaga gcacggcagc attagcacgc tgtcgccgga
     2341 gctgatgtcc gtgccgtacg ccctcgatcg ggttggctct tccgctgaaa tcctccaagc
     2401 tgtttctgag ggtagggtgg tgattctcga caggttcacc agctcgaatc agatgcatca
     2461 gggcgggaag attgaggacg agcaggaacg catagcgttc ctcaagtggc tcgacgagct
     2521 cgagcacgaa gagctcggga ttccgcgttc tgactgcgtc gtctacctga aagttcccgt
     2581 agatatatcg gttaagcttc tcaaggagaa gcgcgccgcg aagaacggaa gcctgaagga
     2641 gggcgagctc gatcaggttg agagcgaccg cctctatctc gagcgcagcc acgcaatggc
     2701 taactggctc gcgcaaaggg agcaaagctg gagtgttatc gactgcacga acgaaagtgg
     2761 agacatgcgt tcgcgcgaag aaatcgctga ggatatattc tcaagcgttg ttaggcaact
     2821 tgaagcctga tatgtggggt cccgcttcgg cggggccctt caggtttggg aaggggtacg
     2881 aggaagggta ctatatagat aagagctaac cggagtatga gatagggtca ctggcgagca
     2941 cgcattacca atttttcact cacccatgag ccagtacgac catatacaga acgaagacca
     3001 ggacgtcttc cgcgcgatag taggggagga gacgcgcgaa gccgaggggc tcgaactcat
     3061 cccgtccgag aactacgtct ccacagccgt tcgcgaagcg atgggcagcg tgctcacgaa
     3121 caagtactca gaagggtacc ctgggaagcg ctattacggc ggacaggaat tcacggacca
     3181 ggtagagacg ctcgcgatcg agcgtgcgaa atctctcttt cgcgctgacc atgcaaacgt
     3241 gcagcctcta ggaggcgctg ctgcgaacat cgccgcgtac ttcgcgtggc tcgagccggg
     3301 agacacgatc cttggcatgg atctcgcgca tgggggacac ctcacgcacg gctcacccgt
     3361 gacgtatatg gcgaagctct acaacttcgt gcgctacggc atgaaggatc cggataccgg
     3421 agagatcgat tacgacgaga tgctgcgcgt ggcgaaagag acccgtccga agatcgtgct
     3481 cgcaggattc tctgggtacc cgcgcaccat cgactggaag cgcgtagcgg atatcgccaa
     3541 agaggtggac gcggtcgcga tggcggacgt cgcgcatatt gcaggtctca ttgcaggcgg
     3601 cgcgcttgag aatccgcttg atcacgggtt ccatatcatg acgacaacca cccataagac
     3661 gctccggggt ccgcgtggcg ccatgatcct ctcgaaggga accgtatcga gccctcttaa
     3721 ggcacctccg aaggcggttg agaaccttcc gacccttatc gatcgcagcg tgttccccgg
     3781 gttccagggc ggtccacaca tgcatcagat cgcagcgaag gcgatcgcgt ttaaggaggc
     3841 ggccacgccg gcgttccgcg agcacgcagc gcaggtgatc aagaacgcac aggcaatggc
     3901 cggtgtgttt atggatcgcg gcgtacgcct catcggtggc gggaccgaca accacctcat
     3961 cctcgcggac gtatatggct cactcggagt ctcaggcggg gaagcggaga cggtgctcga
     4021 tgccgcaggc attacgctta ataagaactc gatcgcaaac gacacccgta agccgttcga
     4081 tccgtccggc atccgcttcg gtacgcctgc catgacgaca cgtgggttta cagaagagga
     4141 ttcgaaggaa gtggcgaaca tcatgctcga cgtgcttaca aagcgcgacg aggaaacggt
     4201 ttctgctgca cgcacgcgcg tacgcgagat tgcgctcgcg catcccgttc cggaaagctt
     4261 catctaggcc tcaccgctcc ttcaggcaga gtgcgcttta ctgggttcgt taggtcgaga
     4321 ttacgaatcc taatcgcacc tgtcatatgc gcacgattgg aaggaccctg atcgagtccg
     4381 cagcgatatt cgccgtagga ttcgctatcg tggggtcaat gctcttcgcg ttttctcaga
     4441 atgctgcagc gtatctcgga taatatccgc gcgtaaagcc gctctcatga gagcggcttt
     4501 tgcgtttgta agcgttccta cggacacacg tctactacaa cggtcggata cct
//
LOCUS       DASWXU010000044         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_238316, whole genome shotgun sequence.
ACCESSION   DASWXU010000044 DASWXU010000000
VERSION     DASWXU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238316"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VGE48_01505"
     CDS             complement(<1..307)
                     /locus_tag="VGE48_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042079.1"
                     /translation="MKRRALIELLLALSLLTVVLAAYGIWYVAVGKASAEAAALSEEI
                     KTKSIDSARVASAKEALESLAADEETMRSYLVRKMDVVSFLERLEDAGGSLGTSIEVV
                     "
     gene            complement(304..858)
                     /locus_tag="VGE48_01510"
     CDS             complement(304..858)
                     /locus_tag="VGE48_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042080.1"
                     /translation="MTPNLTNLLPADRTRSLRRVYFVRLAVVSVLLIAGVAVVHGVLL
                     LPSYLFVQHEVNERRTELALLSERLSGTEEKEVSARVEALGNDAEYLASLATSPKASA
                     AVRAVIGLPREGVRLLGFSFMTNAEGASMTVSGIAATRESLRRFEQTLAAQSYIKTAD
                     LPISAYAKERDIPFMITLTGTLTP"
     gene            complement(848..1939)
                     /gene="pilM"
                     /locus_tag="VGE48_01515"
     CDS             complement(848..1939)
                     /gene="pilM"
                     /locus_tag="VGE48_01515"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY1042081.1"
                     /translation="MASNVLSRLARIEFAAPRYLALPAAGIDVSASGVKLAILEERVH
                     GLELTGFGEARLPLGAVTDGELSDRKSVVEAIRSLSKAHRVKFANIALPESRSYLFEA
                     ITQGEDPRAWKTGLEQKLDEYVPLPPTEVAFDVVPTGPVGSETPVVGVGYARRVIEES
                     LSAFDESNVTVRAVESEMFSMARALLPKGDTETVLIIDIGRTTTKLTVVSSRIPRFAT
                     TLDIGGHALTLAVQKHFGVTEEEAKRVKAEHGLVSSLEEDKGYLEAMLSTVSAIREEI
                     VRRLDYWQSHAAKGSAHEPVTRAILIGGNATVRGLPEYLETSMRIPVELGDVFTNLAS
                     RDHWLPPLDYLESLAYATAIGLALRDYDA"
     gene            complement(1989..2333)
                     /gene="metG"
                     /locus_tag="VGE48_01520"
     CDS             complement(1989..2333)
                     /gene="metG"
                     /locus_tag="VGE48_01520"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEY1042082.1"
                     /translation="MTEETNERISIDDFAKIEVKVGTVRVAEIVPDADKLLRLEVDFG
                     SETRQIVSGIRAYVEDPESLVGRQLSFVTNLAPRKLKGLVSDGMLFAVGEGDSFAFLT
                     PDREVPPGTAAH"
     gene            complement(2317..2724)
                     /gene="ruvX"
                     /locus_tag="VGE48_01525"
     CDS             complement(2317..2724)
                     /gene="ruvX"
                     /locus_tag="VGE48_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY1042083.1"
                     /translation="MRHLGIDFGTKKVGLALSDEAGSMGFPHGILPNDGRLIDEILSL
                     IERKGVEKVVMGESRDYQGNENAVAKDARAFARLIESRSRIEVDFEPEILTTQEARRD
                     PEGVRMTGHAPVDASAAALILTSYLSRNHDRRD"
     gene            2771..3043
                     /locus_tag="VGE48_01530"
     CDS             2771..3043
                     /locus_tag="VGE48_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HEY1042084.1"
                     /translation="MHPFAFTEDLFPFLSAVPAGVLFVLFIVLALWTTVIKAFALWHA
                     ARNGQTFWFVALLVVNSLGILELVYLLAFRKDRREFAAVASDAPAA"
     gene            3057..3142
                     /locus_tag="VGE48_01535"
     tRNA            3057..3142
                     /locus_tag="VGE48_01535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3091..3093,aa:Ser,seq:tga)
     gene            3192..>3622
                     /locus_tag="VGE48_01540"
     CDS             3192..>3622
                     /locus_tag="VGE48_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042085.1"
                     /translation="MARAVFFEWSAEGYLFEEKSSDWYWALGIIAVAAAIASVLFGNV
                     ILALLILVASGTLALSTLKRPRMHLFRVTDEGVMIDENLYEYDSIHSFSVLEYIDPNR
                     PPALSLRTHKILAPHLLIPIVGPDPLELYEFFAEHVDEGKH"
BASE COUNT          710 a         1119 c         1023 g          770 t
ORIGIN      
        1 acaccacttc gatgctcgtc ccgagcgagc cgcccgcgtc ctcgagtcgc tcgaggaacg
       61 agaccacgtc catcttgcgc acgagatagc tgcgcatcgt ctcttcgtcg gctgcgagcg
      121 actcgagcgc ctctttggca cttgcgacac gcgccgaatc gatgctcttc gtcttaatct
      181 cttctgaaag cgcggcagcc tccgcgcttg ccttccccac ggcgacgtac cagataccat
      241 aggccgcaag cacgaccgta agcagagaga gtgcgagcag gagctctatg agtgcgcgac
      301 gcttcatggg gttagggtgc cggttagggt gatcatgaac ggaatgtcac gctcctttgc
      361 gtacgcgctg ataggcaggt ctgctgtttt gatgtaggac tgagcagcaa gcgtttgttc
      421 gaagcgccgg agcgactcgc gtgtcgcagc gatgcccgag accgtcatgc tcgccccttc
      481 cgcattcgtc atgaacgaga agccgagaag ccgcacgcct tcgcgcggaa ggccgatcac
      541 agcacgtacc gcagccgagg ctttcggtga ggtcgcgagg ctcgcgaggt attcggcatc
      601 attcccgagc gcctcgacgc gcgcgctcac ctccttctcc tcggtgcctg aaagtcgctc
      661 agaaaggagc gcgagctcag tcctgcgctc attcacctcg tgctgcacga acaggtatga
      721 cgggagcagc agcaccccgt gcaccaccgc aaccccggca attaagagta ccgagactac
      781 cgcgagtcgc acgaagtaca ccctacgcag ggagcgggta cggtctgcgg ggagaaggtt
      841 ggtaaggtta ggcgtcatag tcacgaaggg cgagcccgat ggcggtcgcg tatgcgagcg
      901 actcaaggta gtccaggggc gggagccagt ggtcgcgaga cgcgagattc gtaaagacgt
      961 caccaagctc taccggaatg cgcatgctcg tctcaaggta ttccggaagg ccgcgtaccg
     1021 tcgcgttccc tcctattagg atcgcgcgcg taaccggctc atgcgcgctt ccctttgccg
     1081 catggctctg ccaatagtcc aatcgacgca cgatctcttc acggatcgca gacaccgtcg
     1141 agagcatcgc ttcgagatat cctttgtctt cctctaaaga ggagacgagc ccatgctctg
     1201 ccttcacgcg cttggcttct tcctcggtca cgccgaagtg tttctgcacc gcaagcgtaa
     1261 gcgcgtgtcc accgatatcg agcgtggtgg cgaatcgagg aatccggctg cttaccaccg
     1321 tgagcttcgt ggttgttcga ccgatatcga tgatgagcac cgtctccgtg tcccctttcg
     1381 ggaggagtgc tctggccatg gaaaacattt cactctccac tgcgcgaaca gttacgtttg
     1441 attcatcaaa tgcggagagt gactcctcaa tcacgcgacg cgcgtagccg acgcccacga
     1501 ccggagtctc agacccaact ggccccgtcg ggaccacatc gaacgccact tcagtaggcg
     1561 gcagcggaac gtactcgtcg agcttctgtt cgagtcccgt cttccacgcg cgagggtcct
     1621 ctccctgcgt aatagcctcg aagagatagc tcctcgattc aggaagtgcg atatttgcga
     1681 acttaaccct gtgagccttc gagagtgagc gtatcgcttc tacgacgctc ttgcggtctg
     1741 agagctcgcc gtccgtcacg gctccaagtg gaagccgcgc ctcaccgaaa ccggtgagct
     1801 cgaggccgtg cacgcgctcc tcgagtatcg cgagcttaac cccgctcgcc gatacgtcta
     1861 tacccgctgc tggaagcgcg agataccgag gcgctgcgaa ctcgatacgt gcgagacggg
     1921 aaaggacgtt agaagccatg cgcaagtagg gaaagtatac cctagcgctg ttatatgaag
     1981 cgcgagcgct agtgtgcagc ggtgcccgga ggaacctctc gatccggtgt aaggaacgca
     2041 aacgagtctc cctcccctac cgcaaacaac atgccatctg agacaagtcc tttgagtttt
     2101 cttggcgcga ggttcgtgac gaacgaaagc tgtctgccca cgagcgattc ggggtcttcg
     2161 acgtacgcac ggatgcccga cacaatctgc cgcgtctcgc ttccgaaatc cacctcaagg
     2221 cgcagcagct tatctgcatc tggcacgatt tcagcaacgc gtactgtgcc gaccttcacc
     2281 tcgatcttgg cgaagtcgtc tatggaaatg cgctcgttag tctcttcggt catggttgcg
     2341 tgataaatag cttgtaagga taagtgcagc cgcgctcgcg tccaccggcg cgtgtccggt
     2401 catacgcacg ccttccgggt cacgacgcgc ttcctgggtc gtcaggatct cgggctcgaa
     2461 gtccacctcg atccgagaac gggattctat aagccgcgcg aaggcccgtg cgtctttggc
     2521 gaccgcgttc tcgttccctt ggtagtcacg ggactctccc atgactacct tctccacgcc
     2581 cttgcgctcg atcaaggaaa ggatctcgtc tattaaccgg ccatcgtttg gaagaatgcc
     2641 gtgtgggaat cccattgagc ccgcttcgtc cgagagcgcg agcccgacct tcttcgtgcc
     2701 gaaatctatc ccgaggtgtc gcatgcgcct atgctacacg ccctctggct cacgtgctac
     2761 actcgccccg atgcatccct tcgcgttcac agaggacctc ttcccgtttc tctcggcggt
     2821 tccggccggc gtgcttttcg tgctcttcat cgtgctcgcc ctctggacca ccgtcataaa
     2881 ggcgtttgcg ctctggcacg cagcgagaaa cggtcagacc ttctggttcg tagcgcttct
     2941 cgtggtgaac tcgctcggta tccttgagct tgtgtacctg ctcgcgttca ggaaggaccg
     3001 ccgggaattt gcggcagttg catcagacgc tccagcagcg taaaccatat agacacggag
     3061 gagtgcgtga gtggtcgaaa cgaactgtct tgaaaacagt caggcccaaa aggtctcgtg
     3121 ggttcgaatc ccacctcctc cgcacaatgt aggcagttcg tgcgcatgcg agtgctatga
     3181 ttcccgtatc tatggcacgc gcagtgttct tcgagtggag tgcggaaggc tatctcttcg
     3241 aggagaagag cagcgactgg tattgggcgc tcggcatcat cgcggttgcg gcggcaatcg
     3301 catccgtact ctttggaaac gttatcctcg cgcttctgat cctcgttgca agcggcacgc
     3361 tcgcactctc aaccttgaag cgtccgcgca tgcacctctt ccgcgtcacg gacgagggcg
     3421 tgatgatcga tgagaatctg tacgagtacg acagtatcca ttccttctca gtcctcgagt
     3481 acatcgatcc gaaccgtccc cctgccctgt cgctgcgcac ccacaagatc ctcgcgccac
     3541 atctcctgat tccgatcgta ggaccagacc cgctcgagct gtatgagttc ttcgctgagc
     3601 atgtggacga aggtaagcac ga
//
LOCUS       DASWXU010000045         8825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_806619, whole genome shotgun sequence.
ACCESSION   DASWXU010000045 DASWXU010000000
VERSION     DASWXU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806619"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VGE48_01545"
     CDS             complement(<1..354)
                     /locus_tag="VGE48_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY1042086.1"
                     /translation="MHPELLLIDKPKGITSFGVIARLRKALNTRKMGHAGTLDPLASG
                     LMLVGIGKGTKKLAELIKLPKTYEAEIVLGERRSTGDMEGTILEESVARNIPEDLVRS
                     TLSDMEGIIELQVPAF"
     gene            417..2459
                     /locus_tag="VGE48_01550"
     CDS             417..2459
                     /locus_tag="VGE48_01550"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1042087.1"
                     /translation="MTKTSMISFLRRAVHRHWKIGLLLFFLYLGALWVSSVFLPNIAI
                     LFPASAIAVSVLFFAGARIWPVVYVASFISNLVLGSPLIYLLIMPFAQALQAGFGATI
                     MRRAGVDPLFRRTRDILITLAAAMISSVVVPTFGVATRWLNTAVTGEPFGSLSWLYWY
                     TGTVFCFVLIVPFVLRWFAKPRFARALPETLETLVVFGGLLGVCYAIFIADITAIGPF
                     PLVYTLFIPLFWIALRLRPRFTTLALVLVALAALASLYITPNPEPLNDRIFQVQTFMI
                     VLTGIFLIITSLQEDRRIATRLIRSQMATLENAVSRISSESRAKNDFIAILAHELRNP
                     LAPVVSAIELMKETRPKDHEDQETLLMMEERMQTVRHLLDDLLDISRISEGKLSLKKE
                     PTELNAILRRAIVSTETYIDDRHQTVSVRAPKEALYVMGDPVRLEQVFTNLITNASKY
                     SDPGDPIDITLRKDGNSAEITVMDAGIGIPPEMLDSIFLPFQQIEQGSRTRKGLGIGL
                     ALVHNFVTAHEGSIRVMSEGTGLGSQFIVRLPLVEHATNTVSENETNTPVATHGSGPS
                     VLVVDDNDAAAWGIGKLLSLRGARVAYAYDAGQAIEQALSERPDIALLDLGLPDRDGY
                     SVAETLRAQGYTGRLIALTGFSTEDVRVRGKAAGFDAFLVKPAGLAELMEAIPEIA"
     gene            2521..3138
                     /locus_tag="VGE48_01555"
     CDS             2521..3138
                     /locus_tag="VGE48_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042088.1"
                     /translation="MSDKLTFDPSLSFEEMVAFVAQGKGIDLAAFNTSPKGLPDLHKE
                     VTRREILLVRDTDGLIKRIVKNVKIVIVDPARSRTWIEVARSYPNGTIVTKEQEYAIR
                     ETCKYQEEPFNAAVRGLAKELGYHFGVSEPSAALVLLPIGRKNPDYPSSVYPGIITSA
                     TSYWYVLTITEFMAEDMYKVRKDDEDSDGDPNAWVEIHIVAIDHT"
     gene            3188..3802
                     /locus_tag="VGE48_01560"
     CDS             3188..3802
                     /locus_tag="VGE48_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042089.1"
                     /translation="MNRTFTILIGIVFLIGALGFLYFLTPNQPIPVPETETAEVNTYT
                     SDAHGFSLSYPENLSILEYTDDMASIGTLIEGGIDSTADVRAMVLIGESGESLQDTAA
                     RELANLCAADGPTASFSCTGVKSVMPFITNSGMPGFEIYLTGALTDIPSGAVTEVNRG
                     PFYAFVLETGATATKVLIVHAPLNKSIEEADADTIRTIAKTVSR"
     gene            3838..4113
                     /locus_tag="VGE48_01565"
     CDS             3838..4113
                     /locus_tag="VGE48_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042090.1"
                     /translation="MMRLMNKQTTYLLIGLGAIIGLFAGFAIAHMKDRHQDFEQYIEA
                     REVAPDKEAFDRNFEAMARWLEEYKRQHPGATDADAQKAFEDAWRSR"
     gene            complement(4145..4498)
                     /locus_tag="VGE48_01570"
     CDS             complement(4145..4498)
                     /locus_tag="VGE48_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY1042091.1"
                     /translation="MHPYVAPTTKPLYRGTQIVWYILGVIEVLLAFRFVLRLLGANPD
                     AGFSSFIYGISFPFTYPFSAVFGSTVVSGSVFEWTTLLAMAVYWLVAFGIVKLLSMSR
                     AVTTPEAAQKLESQV"
     gene            4576..5046
                     /locus_tag="VGE48_01575"
     CDS             4576..5046
                     /locus_tag="VGE48_01575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY1042092.1"
                     /translation="MAQLSLYYPIQPHIVSHVWGTARSAHRSHGARRHNGIDIEITEN
                     NEIRSPIDGIVTLSGHHKGSAGVFLTIVSSTPSDFPDGKRAHVELTFLHLSATLVEDG
                     ARVSIGTPIARSGSGHVHLAPKRVRKGFFGFRDIDRNDADNTFDPEPYWNGRYA"
     gene            complement(5043..5585)
                     /locus_tag="VGE48_01580"
     CDS             complement(5043..5585)
                     /locus_tag="VGE48_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEY1042093.1"
                     /translation="MTQLSAPSKIYIDDSVIAGAGRGVFAKETIKEGELIEACPVVAL
                     EDRKDRDRLRKTGLVNYYFLWGEKRDHAAICLGWGAVYNHSFSPNARFEKHLEDNRMD
                     FHAIRDIAEGEEIVVNYNGDPRNTSPLLIPGIPAEAGGMPRYKTPRIIRGILRRIRLA
                     LSWFAERPVGSFALALAAAA"
     gene            complement(5587..5997)
                     /locus_tag="VGE48_01585"
     CDS             complement(5587..5997)
                     /locus_tag="VGE48_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY1042094.1"
                     /translation="MSSSKFTPITVPLLVKRSSAGLGLFAGAPIKKGSCVIEYVGREL
                     SEEEATTSKSKYLFEISKKRTIDGKPKINKAGYINHSCEPNCETEISRGRVFVMAIKN
                     IKEGDELTYDYGEEYCLEHCVPCRCNAKKHLYSK"
     gene            6089..6442
                     /locus_tag="VGE48_01590"
     CDS             6089..6442
                     /locus_tag="VGE48_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1042095.1"
                     /translation="MRILIVDDNRAAADALGKLLTLRGHVVKTAYSGESAIREAPLFQ
                     PKAIILDIGLPDIEGYDVARAIRSDESVKASLIALTGYGQESDLRKAEKAGFSHHLVK
                     PAGLKEIETVLAEIV"
     gene            6446..7324
                     /locus_tag="VGE48_01595"
     CDS             6446..7324
                     /locus_tag="VGE48_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY1042096.1"
                     /translation="MQVRILAGVSIALGILSIGLGIWALSLSGELSTLRASFIAAKAQ
                     HASELAALSEELRAQAQEGTLLAEALEREQQKAGTLEDDLDDAKDTVKTLTKQVSMDP
                     ELLAKYSKVYFLNENYSPPSFSKVPEEFGFESDRTYEVHKDMLPFLEDLLEDSKKEGL
                     SLRVASGYRSFDTQASLKAGYKVRYGSGANAFSADQGYSEHQLGTTVDFTTPTVGGMF
                     VGFDRTKEFEWLENRAWRYGFILSYPKNNGYYQYEPWHWRFVGKDLAERLHDDKKAFY
                     ELDQRVIDGYLGELFD"
     gene            7346..7693
                     /locus_tag="VGE48_01600"
     CDS             7346..7693
                     /locus_tag="VGE48_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042097.1"
                     /translation="MNERIPTPIPANNNEVPVPDAIITPAVKRETRVEVDGIEINGPK
                     LSELLVTLQRVYETSPHLLSRELVDHARQELVAMSQSDLAKLLNNSNPARWESHPHFY
                     QALFEQLFGPDNA"
     gene            complement(7723..>8825)
                     /locus_tag="VGE48_01605"
     CDS             complement(7723..>8825)
                     /locus_tag="VGE48_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY1042098.1"
                     /translation="WRTEAGTKDAKKNLAKMTEVNPFAFSVKADGSLADTLKIEGKDW
                     QRLIRDAEKKDVLVIPTIMWSDTDNIHNVLSNPTTRASHVKAIKDLVEKHDFDGIDID
                     YEGKKAETRVHFSAFLRELNDALGRKQLDCTIEARMPLSARFSGTPPANIEYANDLPE
                     INKVCDRVRIMTYDQQTADVELNAKARAKNDVYAPVADVAWVKKVVDYMGADIDKRKM
                     TLGVATYGYIYQLMPNTNGNGYGYIKMEAFNPKYATDLAKKLKITPERNSAGELSFSY
                     VPKDQPSSLPSHAELVRHAPKGTSSANAAAAGALALAKKEGRQAPVTYVTWSDSVAIK
                     QKVDLAKREGLAGIAIFKIDGGSDPKLWSVLK"
BASE COUNT         1922 a         2517 c         2366 g         2020 t
ORIGIN      
        1 gaacgcgggc acctggagct cgataatgcc ttccatatct gagagtgtgc tgcgtaccag
       61 gtcttcaggg atgttccttg ctaccgattc ttcgaggatc gttccttcca tatctcccgt
      121 gctcctcctt tctccgagca cgatctctgc ctcataggtc ttcgggagct tgatgagttc
      181 cgcgagcttc ttggttccct tcccgatgcc tacgagcata agaccagacg cgagcgggtc
      241 gagggtgcct gcgtgcccca tcttgcgcgt attgagcgcc ttccgaaggc gcgcgattac
      301 gccgaaggac gtaatgccct tgggcttatc tataagcagc agttcgggat gcatggaggt
      361 actctatcag agcctattca tgtagacttc atgaataaaa gcggatagtg cccgcgatga
      421 ctaagacttc catgatctcc ttcctccgca gagccgtgca tcgccattgg aagattggtc
      481 tactcctctt cttcctgtac ctcggcgcac tctgggtctc gtccgtattc ctgccgaaca
      541 ttgccatcct cttccctgcg tccgctattg cggtctcggt actgtttttt gcaggggctc
      601 gcatctggcc tgtagtctac gttgcatctt tcataagcaa cctggtgctt ggctctccgc
      661 ttatatacct cctaatcatg ccgttcgcac aggcgcttca ggcggggttt ggcgcgacta
      721 tcatgcggcg tgccggcgtc gatcccttat tcaggcgcac acgcgacatt ctcattacgc
      781 tcgcagcagc aatgatttcg tcagtggtgg taccgacttt cggggttgcc acacgctggc
      841 tcaacaccgc cgttacgggt gagccgtttg gaagtctctc ctggctctat tggtacacgg
      901 gtacggtgtt ttgctttgta ctcatcgtcc cgttcgttct gcgctggttc gcaaagcctc
      961 gattcgcgcg agcgctcccg gagaccctcg agacgctcgt cgtctttggc gggcttcttg
     1021 gtgtgtgtta cgcgatattc atcgcggaca ttactgccat cggtccattc ccgctcgtct
     1081 acactctctt tattccgctc ttctggatag cactccgcct gcgtccgcgc ttcactacac
     1141 tcgcacttgt cctggttgca ctagccgcgc tagcaagcct ctatattacg ccgaacccgg
     1201 agccgctcaa cgatcgcatc ttccaggtgc aaacgttcat gattgtactc acgggtattt
     1261 tcctcatcat cacctcactc caagaagatc gtcgcatcgc aaccagactc atccgctcgc
     1321 agatggccac cctcgagaac gcggtgtcac ggatctcgtc agagtcccgc gcgaagaatg
     1381 acttcatcgc catacttgcg cacgaactcc gtaatccgct cgcgccggtg gtgagcgcaa
     1441 tcgagctcat gaaggagacc cgtccaaagg accatgaaga tcaagaaacg ctcttaatga
     1501 tggaagagcg catgcagacc gtacgtcatc ttctcgatga tctcctcgac atttcccgca
     1561 tctctgaagg gaagctctcc ctcaagaagg agccaacgga actgaacgcg atcctccgga
     1621 gagcgatcgt ctcgaccgag acgtatatcg atgaccgaca ccagaccgtc tcggttcggg
     1681 ctccgaagga ggcgctctac gtgatgggag atccggtgcg tctcgagcag gtgttcacga
     1741 atctcataac aaatgcctca aagtactcgg atccaggaga ccctatcgac ataacgcttc
     1801 gcaaggatgg taattctgcg gagataacgg tgatggatgc agggataggc atcccgccag
     1861 agatgctcga ttcaatattc cttcccttcc aacaaatcga acaggggtcg cgcacccgaa
     1921 aggggctcgg tatcggactt gcactcgtac ataacttcgt cacagcacac gaaggttcca
     1981 tacgtgtgat gagtgaaggc acgggacttg ggagccagtt tattgtccgt cttccgctcg
     2041 ttgagcatgc gactaacact gtttctgaaa acgagacgaa cactccagtt gcaacgcacg
     2101 gttccggacc atcagtgctc gttgttgacg acaacgatgc agcagcctgg ggtatcggca
     2161 agcttctgtc gctccgcggc gcacgcgttg cctacgcata tgacgcgggg caagctatcg
     2221 aacaggcgct gagcgagcgt cctgacatcg cactgcttga tctaggcctt ccagatcggg
     2281 atggctactc agttgcagaa accttgcgtg cgcagggtta tacaggccga ctcatagcac
     2341 ttaccggatt tagcacggaa gacgtgaggg ttcgcgggaa ggctgctgga ttcgatgcat
     2401 ttctcgtgaa gcctgcagga ctcgccgagc tcatggaggc gattccggag atcgcgtaaa
     2461 gcttgatttc ccttattttt taaggcatag tcaacccgga ccttagaaga ggaggcgtcc
     2521 atgagcgaca agctcacctt cgacccctcc ttgtcttttg aggagatggt cgcattcgtg
     2581 gcgcaaggaa aaggaatcga ccttgccgca ttcaacacaa gcccgaaagg cttgcctgac
     2641 ctccacaagg aagtgacgcg gcgcgagata ctactcgttc gtgacaccga cggactcatc
     2701 aagcggatcg tcaagaacgt gaaaatcgtg attgtcgacc ctgcacgaag ccgcacctgg
     2761 atcgaggttg cgcgcagcta tccgaacgga accattgtca cgaaggaaca ggaatacgcc
     2821 atccgtgaga cgtgcaagta ccaggaagaa cctttcaacg ccgcggttcg cggcctcgcg
     2881 aaagaactcg gatatcactt cggagtctct gaaccttcgg cagctctggt gctgctcccc
     2941 attgggagaa agaacccgga ctacccttcg tccgtctatc ccggaatcat cacttcggcg
     3001 acgtcgtact ggtacgtgct gaccattacg gagtttatgg cagaggatat gtacaaggtt
     3061 cggaaggatg acgaggacag cgatggcgac cctaacgcat gggtcgagat ccacatcgtc
     3121 gcgatcgatc acacatagcg gcctcccatc ctgggtggcc gctatttcct taggtatact
     3181 ggtttgtatg aaccgcacct tcaccatcct catcggtatc gtcttcctta tcggcgctct
     3241 gggctttttg tatttcctaa ccccgaacca gcccataccg gtcccagaga ctgagaccgc
     3301 agaggtcaac acgtatacca gcgacgcaca cgggttctcg ctctcctacc ccgaaaatct
     3361 ttccattctc gaatacacgg acgacatggc gtccataggc acgctgattg aaggcggtat
     3421 cgacagcacc gcagatgtgc gtgcaatggt gctcataggt gagtctggag agtccttaca
     3481 ggacaccgcc gccagagagc tcgcgaatct gtgcgccgcc gacggcccta ccgcctcatt
     3541 ttcatgcacc ggcgtgaaga gcgtcatgcc gttcataacc aattcgggca tgccgggctt
     3601 tgagatatac ctcaccggcg cgctcacgga tataccgtca ggagccgtga ctgaggttaa
     3661 tcgcggcccg ttctacgcat ttgtgcttga gaccggagca accgcaacca aagtccttat
     3721 cgtgcacgca ccgctcaaca agagcatcga ggaggctgat gccgatacta ttcgcactat
     3781 cgcaaaaacg gtttctcgat aatgaaggct tcagcaaggc ttcatgaacg ctagggcatg
     3841 atgcgtctga tgaacaagca gactacctat ctccttatcg gccttggcgc tatcattggc
     3901 ctctttgcag gatttgccat cgcgcacatg aaggatcgtc atcaggactt cgaacagtac
     3961 attgaggcgc gcgaagtagc tcctgacaaa gaggcgtttg ataggaactt cgaagctatg
     4021 gctcgctggc tcgaggaata caagcgtcag cacccaggtg caaccgatgc ggacgcccag
     4081 aaagccttcg aggatgcttg gcgaagtcgc tagcaaaaag ccccaggttc tcctggggct
     4141 tttgctatac ctgagactcg agtttctgtg ctgcctcagg cgtggttacg gcccgactca
     4201 tggagagaag cttcacgata ccaaaagcga cgagccagta taccgccatc gcaagtaacg
     4261 tggtccattc gaacacgctt ccggacacta cggtcgatcc gaacacggca gagaatggat
     4321 acgtgaacgg gaacgagatt ccgtaaatga aggacgagaa acctgcgtct ggatttgcgc
     4381 caaggagacg cagcacaaaa cgaaacgcga gcaacacctc gatgacaccg aggatgtacc
     4441 agacgatctg ggtgccacgg tagaggggct tagttgttgg agcgacgtac ggatgcataa
     4501 atgagcgtta ccgaatacgc gtatagcgtg gcactcccgt gctgaagaca tcatgaatgc
     4561 ggtacgataa gggcaatggc gcaactctcc ctctattacc caattcagcc acatattgtg
     4621 tcgcatgtgt ggggcactgc tcgctctgcg cacagaagtc atggtgcacg aaggcataac
     4681 ggaatcgata ttgagatcac cgagaacaat gagatacgct cacccatcga cggcatcgtg
     4741 acgctttcgg ggcatcataa aggtagcgct ggcgtattcc tcaccatcgt ctcctctact
     4801 ccctcagatt tcccggatgg caagcgtgcc catgtagaac ttacgttctt acatctctcc
     4861 gccacgctcg tcgaggacgg cgcacgcgta tccatcggta ctccaatcgc acgtagcggg
     4921 agtggccatg tgcacctcgc tccaaagcgc gttcgtaagg gattcttcgg attcagggac
     4981 atcgaccgca atgatgcaga caatacgttc gacccagagc cctactggaa cgggcgctac
     5041 gcttaagcgg cggcggcaag cgcgagtgca aatgaaccga ccggtcgctc tgcgaaccac
     5101 gagagcgcga gccgaatcct gcgcaagatg cctcggataa tgcgcggggt cttataccgc
     5161 ggcattcccc cagcttctgc cgggatgccc ggaatcagaa gcgggctcgt gttgcgcggg
     5221 tctccgttgt agttcacaac gatctcttct ccttccgcga tgtcgcggat cgcatggaaa
     5281 tccatccgat tgtcttccag atgcttctcg aagcgtgcgt tcggtgagaa tgagtggttg
     5341 tataccgcac cccatccaag acagatagcc gcatgatctc gtttctctcc ccagaggaag
     5401 tagtaattca caagacccgt cttccgaaga cgatcacggt ctttcctgtc ttcaagcgcc
     5461 actaccgggc acgcttcgat gagctcgcct tctttaattg tctcttttgc aaatacgccg
     5521 cgtccggctc ccgcgatgac tgaatcatca atataaatct ttgagggtgc cgaaagttgc
     5581 gtcatgctac ttgctataga ggtgcttctt cgcgttgcac cggcacggta cgcagtgctc
     5641 gaggcagtac tcctcgccgt agtcgtacgt gagctcgtcc ccttccttga tgttcttgat
     5701 agccatcacg aacactctgc cgcgacttat ctcggtctcg cagttaggct cgcaggaatg
     5761 attgatgtag cctgccttgt tgatcttcgg cttcccgtct atggtgcgct tcttcgatat
     5821 ctcgaagaga tacttactct tgctcgtagt ggcttcctct tcggagagtt cgcggccgac
     5881 gtactcgatg acgcaggagc ctttcttgat aggcgcgccc gcaaagagcc cgagtcccgc
     5941 gcttgatcgc ttaacgagaa gcggcacggt aatcggggtg aatttggaag aagacatagt
     6001 ttccccatac tacggcctct gaggataccc gcgcaagccg tttgcgtctt atcacgttcg
     6061 cgcgcatgat agggatatgg aaagcggtat gaggatcctc atcgttgacg acaaccgggc
     6121 ggcggcggac gcactcggaa agctccttac gttgcgcggg catgtcgtga agaccgcata
     6181 ctccggagag tccgctatcc gcgaggctcc cctgttccag ccaaaagcca ttattctcga
     6241 tatcgggctc cctgatattg aagggtatga cgtggcgcgt gccatccgtt cggacgagtc
     6301 cgtaaaagcc tcgcttatcg cgcttacagg atatggccag gaatcagacc tccgaaaggc
     6361 tgagaaggcc gggttctcgc accacctcgt aaagccagca gggctcaagg agatcgaaac
     6421 ggtgcttgca gagatcgtat agcgtatgca ggtccggata ctcgccggag tttctatagc
     6481 gctcggaatt ctttccattg gactcggtat ctgggcactc tccctgtccg gagagctttc
     6541 tacgcttcgc gcgtcgttca tcgctgcgaa ggcgcagcac gcttccgagc ttgcggcgct
     6601 ctccgaggaa ctgcgcgcgc aggcacagga aggaacgctt ctcgctgaag cgctcgagcg
     6661 cgaacagcag aaagcaggga ccctcgaaga cgatctcgat gacgcgaagg atacagtaaa
     6721 gacccttaca aagcaggtat cgatggaccc cgagctcctc gcaaagtact cgaaggttta
     6781 tttcttgaat gaaaactatt ccccaccatc gttctcaaag gttccggagg aattcggatt
     6841 cgaaagcgac cggacctacg aggtacataa agatatgctt ccctttcttg aggatctgct
     6901 cgaagactca aagaaggaag gcctgtcgct cagggtggca tccggatacc gctcgtttga
     6961 tacacaagca tcactcaagg ccggatacaa ggtccgctac ggctcaggag cgaatgcctt
     7021 ttcagcagac caggggtatt ccgagcatca gctcggtacc acggttgatt tcacgacgcc
     7081 aacggtcggc gggatgttcg tagggttcga tcgcacgaag gagttcgaat ggctcgagaa
     7141 ccgagcgtgg cgctacgggt tcatactctc ctatccgaag aacaacggat actaccaata
     7201 cgagccctgg cactggcggt tcgtaggcaa ggatcttgcc gaaaggctcc acgacgacaa
     7261 gaaggcgttc tatgagctgg atcagcgagt catagacggg tatctgggag agctgtttga
     7321 ttaggaattt tgtatactac accgcatgaa cgagcgtatt ccaactccaa ttcctgctaa
     7381 caacaacgaa gtacctgttc ctgacgcgat tatcacgcca gccgtgaagc gcgaaacgcg
     7441 tgtggaagtt gacggtatcg aaatcaacgg acctaagctt tctgaattgc tcgtgacgct
     7501 tcagcgagtt tacgagacga gtccacactt actatctcga gaattggtag atcatgccag
     7561 gcaggagtta gttgcaatgt cccaatcgga tctggcgaag ctgctcaaca atagcaaccc
     7621 tgcacgatgg gagtcacatc ctcatttcta ccaagcactg tttgaacagc tattcggacc
     7681 ggataacgct taagaaaagc cccgcttaag cggggctttt ctttacttaa ggacgctcca
     7741 aagtttcgga tccgacccac cgtctatctt gaagatagcg ataccagcga gaccctccct
     7801 cttcgcgagg tctaccttct gcttgatagc gaccgaatcg ctccaggtaa cgtacgtaac
     7861 gggtgcctgg cgaccctcct tcttcgcaag cgcgagcgcg ccagcggcag ctgcgtttgc
     7921 gctggaggtt ccctttggtg catggcggac gagctctgcg tgactcggga gcgaggacgg
     7981 ctggtccttc ggcacgtagc tgaaggacag ctctcctgcc gagtttcgct caggagttat
     8041 cttgagcttc ttggcgaggt cggtcgcata cttcgggttg aacgcttcca tcttgatgta
     8101 gccgtatccg ttgccgttcg tgttaggcat gagctgatag atgtagccgt aggtagcaac
     8161 gccgagcgtc atcttacgct tgtcgatatc agcccccatg taatcgacca ccttcttcac
     8221 ccacgcgacg tccgctaccg gcgcgtacac gtcgttcttc gcgcgcgcct tcgcattcag
     8281 ctccacgtcc gcggtctgct ggtcgtaggt catgatgcgc acgcggtcgc acaccttgtt
     8341 gatctcaggg aggtcgttgg cgtactcgat gtttgccgga ggcgtgcccg agaagcgcgc
     8401 ggaaagcggc atacgcgcct cgatcgtaca gtcaagctgc tttctgccga gtgcgtcatt
     8461 aagttcgcga aggaatgcgg agaagtgcac ccgggtctcg gctttcttgc cttcgtagtc
     8521 gatatcgatg ccgtcgaagt catgcttctc gacgaggtcc ttgattgcct tcacgtgcga
     8581 cgcgcgcgtc gtggggttcg agaggacatt gtggatgtta tccgtatcgc tccacatgat
     8641 agtcggaatc acgagcacat ccttcttttc agcgtcccta atgaggcgct gccaatcctt
     8701 gccctcgatc ttgagggtat ctgccaggct accgtccgct ttcacactga acgcgaaagg
     8761 attaacctcc gtcatcttcg cgaggttctt cttcgcgtcc ttggtacccg cttccgtcct
     8821 ccagt
//
LOCUS       DASWXU010000046         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_660421, whole genome shotgun sequence.
ACCESSION   DASWXU010000046 DASWXU010000000
VERSION     DASWXU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660421"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VGE48_01610"
     CDS             <1..131
                     /locus_tag="VGE48_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEY1042099.1"
                     /translation="LLILWLVGVVSSYTLGGFLHLLLVIAIIVVLVRLIQGKNPIA"
     gene            398..1798
                     /locus_tag="VGE48_01615"
     CDS             398..1798
                     /locus_tag="VGE48_01615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1042100.1"
                     /translation="MDQKKNSRSRGNRGGAPMNRGRGGRPAPVSPAFEAGMKFALDNG
                     WKRPATAPVEPKRSRARSGGPRKGGASSARGQRPERGSRGGGRSGGRNYQEMPVDLSR
                     LINKAKPEEKIEEYVPQHAFDGFAVDERLKENIRAKGYSIPTPIQDQSIPHVLLGKDV
                     VGIANTGTRKTASFLIPLIDKVLKFRELGQEERVLVMVPTRELAIQIEEEFFGFAKRL
                     GVTSVTCVGGANIVPQIRVLRRNPAFVIGTPGRLKDLMERGELDLSAFGTVVLDEADR
                     MLDMGFIEDMRFIMGKMRAERHTLFFSATMSKDIQRLIGDFLNDPVHVSVKTRETSHT
                     IDQDVVVAGHHEKFDKLVELLKQPHFEKTLVFGRTKHGVERLAKDLRRAGIKAESIHG
                     NKTHGARQKALIGFKKNEVNVLVATDVAARGLDISGVSHVINYDLPSTYEDYTHRIGR
                     TGRAGKAGQALTFIPQ"
     gene            1883..2572
                     /locus_tag="VGE48_01620"
     CDS             1883..2572
                     /locus_tag="VGE48_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY1042101.1"
                     /translation="METKENAGAGAFFEKYGTALAVLVGALIIGGAFAFGQGGDRSGQ
                     PAEPIAVDIKDVNVDDSPYVGDKNAKAVVAVWFDYQCRFCKQFETTTLKQVIDTYGDR
                     VKIVYKDFQFLGVASNDAALYSRAVWEAYPDRWDEWFAGMFEGGEESTLDVAGMDALS
                     QRLGLDTARIEKLRTDKATEYQQKIDADRAEGQAFGITGTPGTIIGTALLAGAQPFAN
                     VQSLIEAELNK"
     gene            2632..3138
                     /locus_tag="VGE48_01625"
     CDS             2632..3138
                     /locus_tag="VGE48_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042102.1"
                     /translation="MTRKKVTVNDLMQRGYTYVRSEPEGKNFDPEFAPDLTPYEVLKL
                     GAFGGKYMTDCKDEFPDWSEGVKLCHERHIASYNYFGVNASQPLSVWRKKGWIYEEDP
                     RGWFQWYCRYYSGRRIPEEDKRQIKRWKQMVRHVAQVKYGCRKGDHTCRPRQRQALLH
                     WAYDSRKL"
     gene            3141..3551
                     /locus_tag="VGE48_01630"
     CDS             3141..3551
                     /locus_tag="VGE48_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY1042103.1"
                     /translation="MEMSEVEFEQIAEGEWARIPERFRERVKNVALLIEDDVDDEIRK
                     LEGLEEGESLLGLYRGIPLTERGSEYGTGMTLPDTITLYRLPILEEASEMDRAFRDAV
                     RIVVRETIWHEVGHYFGLDEHSINEREGEDTNRF"
BASE COUNT          813 a          987 c         1103 g          777 t
ORIGIN      
        1 tactcctcat cctctggctc gtgggcgtcg tatcctcata cacgcttggc ggcttcctgc
       61 atcttctcct cgtcatcgcc atcatcgtcg tgctcgtccg ccttatccaa ggcaagaatc
      121 ctatagcgta gctacttata cgaagctttc gatatgttct gaaaggcccg gtacatgccg
      181 ggcctttcta cacccctgca ttgcgctatt ctccagattc tggtatatta catccacaac
      241 ggttgctcca cgtacgtcgt cttgaagcgt agtttcagga cgagggaaga gcgcgaatcg
      301 cgcctcctct aacacgctcg agaacggttg agccaacatc ctcgggctct tttatgcgag
      361 gatcgcgcag tacgctgcgc atctagagaa acacacaatg gatcagaaaa agaattcgcg
      421 ttcccgcggc aatcgcggtg gcgcaccaat gaatcgcggt cgcggcggac gtcctgcgcc
      481 cgtaagccct gcgtttgagg caggaatgaa gtttgcgctt gataacggat ggaagcgtcc
      541 ggcaaccgca ccggttgagc cgaagcgctc tcgtgctcgc tctggcggcc ctcgcaaggg
      601 cggcgcatct tccgcacgtg gtcagcgtcc tgagcgcggc tctcgcggcg gcgggcgctc
      661 tggcggccgc aactaccagg agatgccggt agacctctca aggctcatca ataaggcgaa
      721 gcctgaagag aaaattgagg agtacgtacc acagcatgcc tttgatggct ttgcagtgga
      781 cgagcgtctc aaggaaaaca ttcgcgcgaa gggatacagc atccctacgc ctattcagga
      841 tcagtcgatc ccgcacgtgc ttcttggaaa ggatgtggtg ggtattgcga acactggtac
      901 cagaaagacc gcgtccttcc tcattccgct tatcgacaag gtattgaagt tccgcgagct
      961 tggtcaggag gagcgtgtgc tcgtcatggt tccgacccgc gagcttgcta tccagatcga
     1021 agaggaattc tttggattcg caaagcgcct tggcgttacg tcagttacct gtgtgggtgg
     1081 tgcgaacatt gttccgcaga tccgtgtgct tcgcaggaac ccggctttcg tgatcggaac
     1141 cccggggcgt ctcaaggatc ttatggagcg cggcgagctc gacctctctg cgttcggtac
     1201 tgtagtgctc gacgaggcgg atcgtatgct cgacatgggc ttcatcgaag acatgcgctt
     1261 tatcatgggc aagatgcgcg cggagcgtca tacgctcttc ttctcggcga cgatgtcgaa
     1321 ggacatccag cgcctgatcg gtgacttcct caacgatccg gtgcatgtgt cagttaagac
     1381 ccgcgagacc tcgcatacta tcgaccagga tgtcgtggta gcggggcatc atgagaagtt
     1441 cgacaagctc gtcgagctcc tcaagcagcc gcacttcgag aagacgctcg tgttcggtcg
     1501 caccaagcac ggcgtggagc gccttgcgaa ggatctccgt cgtgcgggca tcaaggctga
     1561 gtccatccat ggaaacaaga cccatggcgc gcgtcagaaa gctctcatcg gcttcaagaa
     1621 gaacgaggtg aacgtgctgg tcgcgactga cgtcgccgca cgtggtctcg atatctccgg
     1681 tgtgtcgcac gtgatcaatt acgatctccc atcgacctat gaggattaca cccaccgcat
     1741 cggccgtacc ggccgcgcag gtaaggcagg tcaggctctg accttcatcc ctcagtaata
     1801 ggaatgccga aaaccccggg acagtgtgtc ccggggtttt ggtatactta ggggcatagc
     1861 attaagggca gatacttatc ttatggaaac caaggaaaat gctggcgcag gcgccttctt
     1921 cgagaagtac ggcaccgcgc tcgcagtgct cgtaggcgcg cttatcatcg gcggcgcatt
     1981 tgcgttcggt cagggcggcg atcgttccgg tcagccggct gagccgattg cagtcgatat
     2041 taaggacgtg aatgtcgatg acagtccgta cgtcggagac aagaacgcga aggcagttgt
     2101 agcggtctgg ttcgattacc agtgtcgctt ctgcaagcag ttcgagacca caaccttaaa
     2161 gcaggttatc gacacctacg gtgatcgcgt aaagatcgtc tacaaggact tccagttcct
     2221 tggtgtcgca tcaaacgatg cagccctata ctcgcgtgct gtttgggagg catacccaga
     2281 tcgctgggat gaatggttcg caggtatgtt cgagggtggc gaggaaagca cactcgacgt
     2341 agcaggcatg gatgcactct cgcagcgtct cggtcttgat accgcgcgca tcgagaagct
     2401 ccgcacggac aaggcaaccg agtaccagca gaagatcgat gcggaccgtg ctgagggcca
     2461 ggcgttcggt atcaccggta ccccaggcac tatcatcggt accgcgcttc tcgcaggcgc
     2521 acagccgttc gcaaacgtac agtcgcttat cgaggctgag ctcaacaagt agctctttgc
     2581 atcggtgcga gcaaagccgc ctctttgagg cggctttgct actatatgag catgacgcga
     2641 aagaaggtta ccgtgaacga cctcatgcag cgggggtaca catacgtgcg cagcgaaccc
     2701 gaagggaaga acttcgatcc tgagttcgcg cctgacctca ccccgtacga ggtactcaag
     2761 ctcggcgcgt tcggcgggaa gtacatgacc gactgcaagg acgagttccc tgattggtcc
     2821 gagggcgtaa agctttgtca tgagcggcac atcgcttcgt ataactattt tggcgtgaac
     2881 gcgtcgcagc ctctgtcggt gtggcgcaag aagggatgga tatacgaaga agatccgcgt
     2941 ggctggttcc agtggtactg ccggtactat agtggccgac gcattcctga ggaagacaag
     3001 cgccagatta agcgctggaa acagatggta cgtcatgtgg cgcaggtgaa gtacggatgt
     3061 cggaagggag atcacacctg cagaccgcgc cagcgtcagg cgcttctcca ttgggcgtac
     3121 gatagccgaa agctctaggt atggagatga gcgaagtcga gtttgagcag atcgcagagg
     3181 gcgaatgggc acgcataccg gaacgcttca gggagcgtgt gaagaatgtc gcgctcctta
     3241 ttgaggacga tgttgatgac gagatccgga aactcgaggg tcttgaggaa ggagagagtc
     3301 tgctcgggct ctatcgtggc atcccgctca cggagcgtgg gagcgaatac ggcacgggta
     3361 tgacgcttcc ggatacgatc acgctgtacc gactcccaat acttgaagag gctagtgaaa
     3421 tggatcgcgc gtttcgggac gcagttagga tcgtggtgcg tgagacgata tggcacgaag
     3481 tcgggcacta tttcgggctc gacgagcaca gtattaacga gcgggaaggg gaggacacga
     3541 atcgtttcta aatccaagcc gtcacaggga aaacacaaag ccggacccca ggatccggct
     3601 tgcatcaatt agtacgggta cttcgcttca ggagcgcgcg ggagctcaaa cggttcgttc
     3661 gagatctgta tgctgcctgc
//
LOCUS       DASWXU010000047         5158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_660988, whole genome shotgun sequence.
ACCESSION   DASWXU010000047 DASWXU010000000
VERSION     DASWXU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660988"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VGE48_01635"
     CDS             <1..245
                     /locus_tag="VGE48_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020042664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042104.1"
                     /translation="LSYFFLRRDSQFHAFGLFVFAVLASMGMGAINEIIEFAAKLSVP
                     DSNVGGYYNTAVDLTVNFLGAIIGALVASLLWKPKP"
     gene            281..356
                     /locus_tag="VGE48_01640"
     tRNA            281..356
                     /locus_tag="VGE48_01640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:314..316,aa:Thr,seq:cgt)
     gene            423..1289
                     /locus_tag="VGE48_01645"
     CDS             423..1289
                     /locus_tag="VGE48_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEY1042105.1"
                     /translation="MSEDRIAEIEALMASPDFWKDPASAQRVIREYQNLKEGGSGDGH
                     DADSATIGILAGAGGDDAEDFARMLRRMYEGYASKEGWDTWELHANENDHGGFRNVLI
                     EVSGKGAYGKLKNEAGVHRLVRISPFNANAKRQTSFALVEVLPVLSGNEEIVLAPDDL
                     DIQFARSGGAGGQNVNKRETAVRITHTPSGVSAHVSSERSQQANREKAMDLVRAKLFR
                     IEEDKRLAEAKGRSIAATTANEWGSQMRSYVLHPYKLVKDHESGAESHDPEAVLEGDL
                     DLFIDASREAGV"
     gene            1303..1983
                     /locus_tag="VGE48_01650"
     CDS             1303..1983
                     /locus_tag="VGE48_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1042106.1"
                     /translation="MIYFDKVTKIHKDGTPGLEDITIAIAPKEFVSIVGHSGAGKTTL
                     LKMLLAETQPTSGTVFFESTDVHALPKSKLHHYRRKIGMVFQDFRLIPNKTAYENIAF
                     AMEAAGRPEDEIALDVPHILDLVDLGGKAHHFPSQLSGGEQQRVAIGRAIINQPDVIV
                     ADEPTGNLDPVNTYEIMDILKKINELGTTVLITTHNKGVVDAMGRRVITMDRGRVIRD
                     DQQGHYVL"
     gene            2006..2920
                     /locus_tag="VGE48_01655"
     CDS             2006..2920
                     /locus_tag="VGE48_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY1042107.1"
                     /translation="MMMKRVFTGGAKNFARSGAVSFATVLIMTITLCIVGSLIFLSAI
                     LSNTLAGLEDKVDVNVYFVTDADERDIVSIQRQLEAMPEVASVIYTSREDRLAEFRAR
                     HEDDQLTIQALDELGDNPLGASLAVKAQDPTQYAGIVEFLSTDPSVSPNGPIIDSINY
                     FQNKAVIDRLTAAIDATERAGLVIVILFAVASTIIALATIRLAIYTARDEIAVMRLVG
                     ASNAYIRGPFIVAGIISGLTAAVISLLFFYPVTWYAGQSLSTWLGGFNLFDYYLSHFG
                     MVFAILAGTGVLVGALASWLAVRRYLKV"
     gene            2925..4568
                     /locus_tag="VGE48_01660"
     CDS             2925..4568
                     /locus_tag="VGE48_01660"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY1042108.1"
                     /translation="MALQGHKYIFVLGGVMSGVGKGIASSSIALILKQRGYEVNLVKV
                     DPYLNVDAGTMNPTEHGEVFVLDSGLETDQDMGNYERFLNRDLGPEDYMTSGMVYKTV
                     IENERALKYGGKCIEAIPHVRDEILRRIEAAAAHNGSQISVIEIGGTVGDFQNALFIE
                     AARVMRLTHPDDVTFVMVSYLPVPGTLGEMKTKPTQSAVRDLNAYGVQPDFIIARSKE
                     SMDEKRKEKLAIWCNVRPDRIISAPDVESIYDVPMNFEKDNLGDNLLDALKLSKEKAA
                     DFSEWQKFVDGIKIESPEVKIGIVGKYFDTGDFVLSDAYLSVIEAIKFSGSTLRVKPK
                     IEWLNAKDFEKGGDLSKLSEFNGIIVPGGFGETGIEGKIKAIQYCREQKIPYLGICYG
                     MQLMVIEYARNVLGLEGANTAEINTEAPHLIVDVMPEQKALIAEGKYGGTMRLGTYEE
                     RVKDGTIAREAYGADTVHERHRHRYEINPEYVKRIEDAGLVFSATSPDGVLMEVAELP
                     KDMHPFMLGTQAHPELKARPLSPHPLFTAFLKAASEKAA"
     gene            4608..5036
                     /locus_tag="VGE48_01665"
     CDS             4608..5036
                     /locus_tag="VGE48_01665"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEY1042109.1"
                     /translation="MEVVINYWAVLLSAVVAIILGFIWFGPLFGKIWMRTIKIDMPTE
                     MTKAMKQAMMRSYALVAVAALVMAAVLAHALFYVTEFTGLAGVSTGITTALWCWLGFM
                     VAPLMGPVLWEDRPWKYFFITSGYWLIALCLMGTILSVWR"
BASE COUNT         1105 a         1368 c         1523 g         1162 t
ORIGIN      
        1 tgctctcgta tttcttcttg aggcgtgaca gccagttcca tgcgttcggc cttttcgtgt
       61 tcgcggtcct cgcttccatg ggtatgggag cgatcaacga gatcatcgag tttgcggcga
      121 agctctcggt cccggattcg aatgtcggcg ggtactacaa cacggcggtc gacctcacag
      181 ttaacttcct tggcgctatt attggcgcgc tcgtcgcaag tctgctttgg aagccgaaac
      241 cttgaacgga agagcgtttc ccgctatggt agacgggttc gccaccgtag ctcagttggt
      301 agagcatttc actcgtaacg aaaaggtcgc cagttcaatc ctggccggtg gctccagcgc
      361 gaagcgccta cggaattatg gttccgtagg cgcttcgtcc ttaggtaggg tacactcgtc
      421 ctatgtctga ggaccgcatc gcagagatcg aggccttgat ggcctctcca gatttctgga
      481 aggaccctgc gagcgcgcag cgtgtgatcc gcgaatatca gaatttgaaa gagggaggat
      541 cgggagacgg ccacgatgcc gattccgcta cgataggtat ccttgccgga gccggagggg
      601 acgatgcgga ggatttcgca cgcatgcttc gcaggatgta tgagggctat gcgtcgaaag
      661 aggggtggga cacgtgggaa ctacacgcca acgagaacga tcatggaggg ttccgtaacg
      721 tgcttatcga agtatctgga aaaggagcgt acggaaagct gaaaaacgag gccggagtgc
      781 atcgcttggt gcgtatctcg ccattcaatg cgaacgccaa gcgccagact tcgttcgcgc
      841 tcgtcgaggt gctcccggta ctttcgggga atgaagagat agtccttgcg cctgacgatc
      901 tcgatatcca gttcgcgcgc tcaggcggcg ccggaggaca gaatgtgaac aagcgggaga
      961 cggcggtccg tatcacacac accccttcag gtgtatcagc gcatgtgtct tcagagcgca
     1021 gccagcaggc gaatcgcgaa aaggccatgg accttgttcg ggcaaaactc ttcagaattg
     1081 aagaggataa gcgccttgca gaagcgaagg gccgttctat tgcggccaca accgcaaacg
     1141 agtggggcag tcagatgcgt tcctacgtgc tgcatccgta taaattagtg aaagatcatg
     1201 agtcaggcgc cgaatcgcat gatcctgaag cggtattaga aggtgatctg gacctgttca
     1261 tagacgcttc ccgtgaggct ggggtataat ggccttcgtt atatgatcta tttcgacaag
     1321 gtgacaaaaa tccacaagga cgggaccccc ggactcgagg acatcaccat agcgattgcg
     1381 cctaaggagt tcgtttccat cgtgggacac tcgggtgccg gcaaaaccac cctcctcaag
     1441 atgcttcttg cagagacgca gcccacctca ggcaccgtct tcttcgagtc gaccgacgta
     1501 cacgcgctcc cgaagtccaa acttcatcac tatcgccgca agatcggcat ggtgttccag
     1561 gatttccgcc tcattccgaa caagaccgca tacgagaaca tcgcgtttgc gatggaggct
     1621 gcagggcgtc cggaggatga gatcgcgctt gatgtgccgc acatccttga tcttgtggat
     1681 ctcggcggta aggcacacca cttcccaagc cagctctcag gcggcgagca gcagcgcgtc
     1741 gcgatcggtc gcgccatcat caatcagccg gacgtgatcg tagcggacga gccgaccgga
     1801 aacctcgatc cggtgaacac gtacgagatc atggatatcc tgaagaagat caatgaactt
     1861 ggaaccacgg tcctcattac tacgcataac aagggcgtgg tggacgcgat ggggaggcgt
     1921 gtgatcacga tggatcgagg acgcgtgatc cgagatgatc agcaaggcca ttacgtgctc
     1981 taattctcaa attgtaagta tgtggatgat gatgaagcga gtgttcacgg gaggagcgaa
     2041 gaacttcgcg cgcagcggcg ccgtctcatt cgcaaccgtg ctcatcatga cgatcacgct
     2101 ttgtatcgtc ggttccttga tcttcctctc cgcaattctt tctaatacgc tcgctggtct
     2161 tgaggataag gtggatgtga acgtttactt tgttacggac gcggacgagc gagacattgt
     2221 ttccatacag cgacagcttg aggcaatgcc agaggttgct agcgtcattt acacctcccg
     2281 tgaggatcgc cttgcagagt tccgtgcacg acacgaggat gaccagctca ccatccaagc
     2341 gcttgacgag ctcggggaca atccgctcgg tgcgtctcta gcggtgaagg cgcaggaccc
     2401 gacgcagtac gcaggtatcg tggaatttct ttccactgac ccgtcggtct ctccgaacgg
     2461 cccgatcatc gacagcatca actacttcca aaacaaggcg gttatcgacc gtcttacagc
     2521 agcgatcgac gcgacagagc gcgctggcct cgttattgtg attctgtttg cggtagcgtc
     2581 gaccattatc gcgcttgcga ctattcgtct tgcgatctac accgctcgcg acgagattgc
     2641 cgtcatgcgc ctcgtgggcg cctcaaatgc ctacatccgc ggtccgttca tcgttgccgg
     2701 catcatctcg ggacttacgg ctgcggttat ctcgcttctc ttcttctatc cggttacgtg
     2761 gtatgcgggg cagtctctgt cgacctggct tggaggattc aatctctttg attactacct
     2821 ctcgcatttc ggcatggtgt tcgcgatcct cgcgggtacg ggcgtgctcg ttggtgcgct
     2881 tgcgtcatgg cttgcggtgc gccgctacct aaaggtctag gttcatggcg cttcagggtc
     2941 acaagtacat attcgtccta ggcggggtga tgtccggcgt cggtaagggc atcgcctctt
     3001 cttctatcgc gctcatcctg aagcagcgcg ggtacgaggt gaatctcgtg aaggttgacc
     3061 cgtacctcaa cgtagacgcg ggcaccatga acccaaccga gcacggagaa gtgttcgtgc
     3121 tcgattcggg ccttgagacg gatcaggaca tgggcaacta cgagcgcttc ttgaaccgcg
     3181 atctcgggcc cgaggactac atgacctccg gtatggtcta caagacggtt atcgagaacg
     3241 agcgtgcgct taagtacggc ggcaaatgca tcgaggcgat accgcacgtg cgtgacgaga
     3301 tcctccgccg tatcgaagcg gcagcggcgc ataacggtag ccagatctcg gttattgaga
     3361 tcggtggcac cgtgggcgac ttccagaacg cgctcttcat tgaggcggcg cgcgtcatgc
     3421 gccttacgca cccggacgat gtcacgttcg ttatggtgtc ctaccttcca gttccgggca
     3481 cgctcggtga gatgaagacg aagccgacgc agagcgcggt ccgtgatttg aacgcctatg
     3541 gcgtgcagcc agatttcatc atcgcgcgct cgaaggaatc catggatgag aagcgcaagg
     3601 agaagctcgc catctggtgc aatgtgcgcc cagaccgaat catctcagcg cctgacgtag
     3661 agagtatcta cgacgtgccg atgaatttcg agaaggacaa cctcggcgac aacctccttg
     3721 atgcgctcaa actctcgaag gagaaggctg cggatttctc ggagtggcag aagtttgtag
     3781 acgggataaa gatcgagagc cctgaagtga agataggtat cgttggaaag tattttgata
     3841 cgggtgattt cgtactttct gatgcgtacc tttcggttat cgaggcgatc aaattctcgg
     3901 gctcaacgct ccgcgtgaag ccaaaaattg aatggttgaa cgcgaaggat ttcgagaagg
     3961 gaggagatct atccaagctc tctgagttta acggcatcat cgtgccggga ggattcggtg
     4021 agacgggtat cgaagggaag ataaaagcta ttcagtactg tcgcgaacag aagattccgt
     4081 atttgggtat ctgctatggt atgcagctca tggttatcga gtacgcgcgc aatgtgctcg
     4141 ggctggaggg cgccaatacc gctgagatca atactgaagc gccacacctt atcgttgacg
     4201 ttatgccgga gcagaaggca cttatcgccg aagggaagta cggcggcacc atgcgccttg
     4261 ggacctacga ggagcgcgtt aaggacggta ctatcgcgcg cgaggcatat ggcgcagata
     4321 cggttcacga acgccatcgc catcgctacg agatcaatcc ggagtatgtg aagcggattg
     4381 aggacgcagg actcgtgttc tctgcaacca gccctgacgg cgtgctcatg gaagtggcag
     4441 agcttccgaa ggatatgcat ccattcatgc tcggtaccca ggcgcatccg gagctgaagg
     4501 cgcgtccact atctccgcac ccgctcttca ccgcgtttct taaggcagcg agcgagaagg
     4561 cagcgtaggg gtatactggg aaccgtattc cttaattcgt tttcctcatg gaagttgtaa
     4621 tcaattactg ggccgtgctc ctttccgcgg tggtggcgat catcctcgga ttcatctggt
     4681 tcggtccttt gttcggaaag atctggatgc gcaccatcaa gatcgacatg ccgaccgaga
     4741 tgactaaggc gatgaagcag gcgatgatgc gctcatacgc gctcgtagct gtcgctgcgc
     4801 tcgtcatggc agcggtgctt gcgcacgcac ttttctacgt gactgagttc acgggccttg
     4861 cgggtgtctc caccggcatc acgactgccc tctggtgctg gctcggcttc atggttgcgc
     4921 cgctcatggg tccagtcctt tgggaagatc gcccgtggaa gtacttcttt atcacttccg
     4981 gctactggct tatcgcgctc tgcctcatgg gcacgatact ctcggtctgg cggtagggca
     5041 ttgaaactgc ctgggtttcc aggtattgtg tgcgaaccgg gagcggtttc ggccgctcct
     5101 cggtattgcc gggtcgtcta aaggtaggac agcggtttct ggcaccgtca atcggggt
//
LOCUS       DASWXU010000048         9174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_34937, whole genome shotgun sequence.
ACCESSION   DASWXU010000048 DASWXU010000000
VERSION     DASWXU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34937"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..472
                     /locus_tag="VGE48_01670"
     CDS             302..472
                     /locus_tag="VGE48_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042110.1"
                     /translation="MTADEVRNAYHEASALVVAEHAARMDALLERARMRVQGTKDQAR
                     IDEVRARLAKGL"
     gene            469..1104
                     /locus_tag="VGE48_01675"
     CDS             469..1104
                     /locus_tag="VGE48_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042111.1"
                     /translation="MSPYERMPEAAELRPSEEALPNESFESFEQAAGDAELALVAKGL
                     STENELMKLATKQNDGETLAALMQEIREAREESRRFREDLARLRSGTALGLALALPEG
                     DAVLPSLISAQALAEHPERARTLLESVEIAAREREDLIPEAAPQLRTLREETSEALMR
                     VQESGDSRYGTLSMKVAEIAARALANAAGLGLGVAGYDIIKEVTKAYRAKA"
     gene            complement(1101..2774)
                     /locus_tag="VGE48_01680"
     CDS             complement(1101..2774)
                     /locus_tag="VGE48_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY1042112.1"
                     /translation="MPAHFDTDAENAKLASLHEQEEEDLAQLLSQKYGLNYTDLTVVP
                     INVDALRLIPEKEAREAEAVAFEKTGKHLSIAFRNPNTSLLRDIEHALESQGFILTRF
                     LVSKKSLERAYGRYAELSFATETKAGVLDIRGDELAKDREELTSLALLRARLDEVLVT
                     GARAQVSRVLEFVLATAFSLKASDIHIEPEEEGIRLRLRLDGALTDAYTFDAHTYQLL
                     SSRIKLLSGLKLNIRDRAQDGRFSVPVGGSEVEIRTSLIPGAYGESFVMRILDPSILG
                     VSFEKLGIRPNLLERLKTEITRPTGMLLVTGPTGSGKTTTLYAFLRHIHTPEIKIVTI
                     EDPIEYHLPGIVQTQTDGKNYTFASGLRSVLRQDPDVIMVGEVRDGEVAETAVQAALT
                     GHFVFSTLHTNNAAGTFPRLVDLGVDPKTFASAVSVAMAQRLVRTLNPEERKQVPATP
                     EQKALMEKVLSTIEDKSLLPASLDMVWVPDPKSPDSTGYKGRTGVHEAIFMDDELGTY
                     LRENPSEGDIAKSVRRQGFLTMAQDGVLKALSGETSLEEVFRVVDLPRD"
     gene            2833..3276
                     /gene="tsaE"
                     /locus_tag="VGE48_01685"
     CDS             2833..3276
                     /gene="tsaE"
                     /locus_tag="VGE48_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY1042113.1"
                     /translation="MRPAATVTDLEALSAEATAFVSELLPKETGATLVTLSGDLGAGK
                     TSFTQAVAKALGVEDHVTSPTFVLAKSYALPEGLAFKELLHIDTYRLEEGKDLVPLGF
                     FESMEDTGTLVFLEWPEIVADKLPKPDVAITIVANEDGSRTITYA"
     gene            3269..5647
                     /locus_tag="VGE48_01690"
     CDS             3269..5647
                     /locus_tag="VGE48_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY1042114.1"
                     /translation="MHKTRLVLLDAHAIIHRAYHALPDFTAPSGEPTGALYGLSSMLL
                     RIATELRPDYVAACYDLPKPTVRHEAYADYKAGRAQTDDSLATQLTTSRRVFDAFAIP
                     IYEKAGFEADDILGTIVEKLKDNDEVEIVIASGDMDTMQLISGKKVQVYTLKKGLNDT
                     ILYDEAAVTERYGFGPERIADFKGLRGDPSDNIKGVPGIGEKTGTELIQKFGSVEDIY
                     DSIEKDENAVLAAGIKPRIVNLLKEHEEDARFSKSLATIRLDAPISFSFPKKFWKDCI
                     DQAKLIALFDELGFRSLKPRVKSLLTQGGGVDEEDEESFEELPEEEDIPKTRLEEAKV
                     MLWLLYSDFTNPSLDDILAYTKAKSFDEAYAALEKGIAETGRLKEVYEEMERPLIGII
                     EEMHQCGVRIDTAVLEDLRKKYRKELTAIEKRIYKAAGREFNISSPKQLGEVLFDELG
                     LVAKGQKKTSTGQRSTKESELEKIKDLHPIVADVLEFRGLQKLLSTYIEALPPLLWDD
                     GRLHAQFLQYGAVTGRMASQNPGLQNIPMRTERGRAIRHAFVPREGFTLVGLDYSQIE
                     LRIAAMLSGDQKLIDIFKSGRDVHQEVAAAVFNVAPENVDREMRRRAKIINFGILYGM
                     GVNALKTQLGTTTSEAHTFYDDYFETFKTLAQYLESTRGFARKHGYTETLFGRRRQFP
                     EMKSPLPYVRAQAERMAINAPIQGTQADITKRAMVSVDRLLKEMGANEDAHLLLQVHD
                     ELVYEIRNSRVAELAEKIRSEMERALPEEYTEGVPVIAEAKAGSDWGSMEAL"
     gene            5644..6300
                     /locus_tag="VGE48_01695"
     CDS             5644..6300
                     /locus_tag="VGE48_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042115.1"
                     /translation="MIYLFHGSDAGKARAKAFQWVAAARAKAPDAAYIRLEGKEITEA
                     ALTEALSTQGLFFSKSLVLIDDPFAAQESGDIVLSLIDALAASENPVGILAPKLLAAQ
                     AKKLEAKAAKIFKEDSAAKPARGFNSALVNALGSKNGVALWKEIVKAFRSGDVPETVH
                     GLLHWKARDLMQKGSPTWTKEEARALSVSLIELVADSRSGDLPLGPALERFVLSFSNS
                     "
     gene            complement(6301..6373)
                     /locus_tag="VGE48_01700"
     tRNA            complement(6301..6373)
                     /locus_tag="VGE48_01700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6338..6340),aa:Lys,seq:ttt)
     gene            complement(6393..7277)
                     /locus_tag="VGE48_01705"
     CDS             complement(6393..7277)
                     /locus_tag="VGE48_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY1042116.1"
                     /translation="MKSDAVFFLGVLVFFFAAWLYSGGPNRPISFAGPYITPITDVDT
                     NQQGYGDGGSFWGDITNIGNGLTGLQGTNDPSPLRGEVRVSGVNNAGTTDPDLEYVTI
                     QNTGTAPIDITGWRLVSGASGKSGRIPEGAALPRSGRVNPTDRIVLQPGDTALVVTGE
                     SPLGVSFKENRCIGYLSRNQKYQPALPLTCPLASEEFAQHYTGNELRDDTCYYRMQST
                     PSCTTPSRSGVSSRCEDLIDEYLTYNGCVEHHRSDANFVGNTWRIYLDENRELWKPTR
                     DSVKLLDASGKTVDLYTY"
     gene            complement(7297..8562)
                     /gene="murE"
                     /locus_tag="VGE48_01710"
     CDS             complement(7297..8562)
                     /gene="murE"
                     /locus_tag="VGE48_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HEY1042117.1"
                     /translation="MYKILNALIEFLKKILPISAVRPLLPFYHRTLAFLLAASYGFPA
                     KKLTVIGVTGTKGKSSVSDMLFSVLRHAGYPAAVAGTIRFAINDESEPNLYKMTMPGR
                     GFIHAFLAKAVKAGAKYAVVEITSESTRQHRHRYLFQNGLIFTNLQKEHIESHGGFEN
                     YALAKLEIGKELARSPKRPRVIIPNGDDPRSAPYLALSVEEKRPFSYEEASDVELAPG
                     RASFTYKDVRFTLQLPGSFSIMNALAVIKAAEFLEVPLPKIAEGLAALERIPGRTERI
                     EEGQNFQAIVDYAHTPDSLKALYEAYPTRRICVLGNTGGGRDTWKRPEMGKIAETYCD
                     VVILANEDPYDEDPHTIVDAMAAGMDTKPEIIMDRRAAIARALSLAKAGDAVLITGKG
                     TDPYIMEANNVKTPWSDAQVVREELRKIL"
     gene            complement(8565..9107)
                     /gene="ruvA"
                     /locus_tag="VGE48_01715"
     CDS             complement(8565..9107)
                     /gene="ruvA"
                     /locus_tag="VGE48_01715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY1042118.1"
                     /translation="MIRQVRGTVAGTEPGGIILDISGWGAFIYMSAPEAYAEGQEIAL
                     KTYLAVKQDGMDLYGFPDEAERRFFELLVGVPGVGPKTALGILRKAPREALEGAIGTR
                     DLDYLTRVIGLGKKAAEKLLVELSEKIGAGRAHDTDDSEVFDTLVALGYTEREARAAL
                     SKVPASVLGKDARLKAALSK"
BASE COUNT         1943 a         2538 c         2772 g         1921 t
ORIGIN      
        1 tgacataaaa ttgcactatg ctagtattct atcacaccgg ctctttacca gggccgattc
       61 tcttttcaga tcgcacatag caccctctag gcaaggcttg acggcttcag ggcgtttccc
      121 caaggaccat ctggtaccgc acttgtttgc ggagcgctgg gttcatcctc aggcgactcg
      181 ttcaccttcc ttaattggaa ggagtgcctg gagggtactg tgtaggatct cgagtgaata
      241 cgcagtgaga aacgcacgcc aaggcgtgcg ttttgcgtta tcggcgcggt actatgagcg
      301 catgaccgcg gacgaagtga ggaacgcgta tcacgaagcg agtgctctcg ttgttgcaga
      361 gcatgccgcg cgcatggatg cgctccttga gcgtgcacga atgagggtgc agggaacaaa
      421 agaccaggcc cgcatcgatg aggtgcgagc gcgcctcgca aaaggtctat gagtccgtac
      481 gaacgaatgc ctgaagcggc agagctacgg ccgtcggagg aggctcttcc caacgagagt
      541 ttcgaatctt tcgagcaggc ggcaggcgat gcagagcttg cgctcgttgc taaggggctc
      601 tcgactgaga acgaactcat gaagcttgcg acaaagcaga atgacggaga gacactcgct
      661 gcgcttatgc aggagattcg ggaggcacgc gaagagagtc gtcggtttcg cgaggatctt
      721 gcgcggctgc gctccggtac ggcgcttggg cttgcactcg cccttccaga aggggacgcg
      781 gttctcccat ctctcatatc tgcgcaagcg cttgcggagc atcctgagcg cgcgcggacc
      841 cttctcgaat ccgtagagat cgcggcacga gaacgggaag atctcattcc ggaagccgca
      901 cctcaactgc gcacactccg ggaagagacg agcgaggcgc tgatgcgggt tcaggaatcg
      961 ggagacagta ggtacgggac actctctatg aaagtggcag agatcgctgc acgagcgctc
     1021 gcgaacgcag cgggcctcgg cctcggcgtt gctgggtacg acatcatcaa agaggtgaca
     1081 aaagcgtacc gcgcgaaagc ctagtctctc gggagatcaa ctacacggaa cacctcttcg
     1141 agcgaggtct ctcctgaaag cgccttcaat acaccgtcct gtgccatggt caaaaagccc
     1201 tggcggcgaa ccgacttcgc aatatcgcct tcgctcgggt tctcgcgcag gtacgtgccg
     1261 agctcgtcat ccatgaatat cgcctcatgt acgccggtcc gtcccttgta tccagtgctg
     1321 tccggagact tcgggtcagg tacccagacc atatcaagcg acgcaggaag aagactcttg
     1381 tcttcaatgg ttgagagcac cttttccata agtgccttct gctcaggagt tgcagggacc
     1441 tgcttccgct cttcgggatt caaggtgcgc acaagacgct gcgccatggc gaccgatacg
     1501 gcgctcgcga atgtctttgg gtcgacacca aggtctacga ggcgcgggaa ggtacctgct
     1561 gcgttattgg tgtgaagtgt tgagaacacg aagtgtccgg tgagggcggc ttggaccgcg
     1621 gtctctgcga cttcgccgtc gcgcacctct ccgaccatga tcacatccgg gtcctggcga
     1681 agcaccgagc ggagtcccga agcaaaggtg taattcttgc cgtcggtctg ggtctgcacg
     1741 atgcccggga ggtgatactc gatcgggtct tcgatcgtga cgatcttgat ctccggggtg
     1801 tggatatggc gcaggaacgc gtacagcgtc gttgtcttac cagatccggt gggtccggtg
     1861 acgagaagca tgccggtagg gcgggtgatc tctgtcttga ggcgctcaag gaggttcggt
     1921 cggataccga gcttctcgaa cgagacgccg aggatagacg ggtcgaggat acgcatcacg
     1981 aacgattcgc cgtatgcgcc cgggataagc gacgtgcgga tctcaacttc gcttccgccg
     2041 accggtactg agaagcgccc atcctgcgca cgatctcgga tgttgagctt cagtcccgaa
     2101 aggagtttta tgcgcgagga gaggagttga tacgtgtgtg catcgaaggt gtatgcgtct
     2161 gtaagtgcgc catcaaggcg aaggcgaagg cgaatacctt cttcctcagg ctctatgtgg
     2221 atgtcggacg ctttgaggga gaacgcggta gcaagcacga actcaagcac gcgtgatacc
     2281 tgcgcgcgtg cgccggtaac cagtacttca tcaagtcggg cacgaagaag cgcaaggctc
     2341 gtgagttctt cgcggtcttt tgcgagctcg tcgccgcgga tatcaaggac gcccgctttg
     2401 gtctcggtcg cgaacgagag ctctgcatag cggccgtacg cgcgctcgag tgacttcttt
     2461 gatacgagga accgggtgag gatgaaccct tgtgactcga gcgcgtgttc aatgtccctc
     2521 aataaggatg tgttcgggtt acggaacgcg attgaaaggt gtttgccggt cttctcgaac
     2581 gccaccgctt ccgcttcccg ggcctctttt tctggtatga gccggagcgc atccacgttt
     2641 atcggtacga ccgtaaggtc ggtgtaattc aggccatact tctgagagag cagctgcgcg
     2701 aggtcttcct cctcctgttc gtgtagggaa gcgagcttcg cgttctcggc gtcagtatcg
     2761 aaatgtgcag gcattacgcc cataataccg cctcgacgcg cgcgttccta ccgcgcttgg
     2821 tacaatttcc ccatgagacc cgcggcaacc gtgaccgatc tcgaagcgct gtctgcagaa
     2881 gcgaccgcat tcgtctctga actcctcccg aaagagacgg gagcaacgct tgtgaccttg
     2941 tctggagacc tgggggcagg gaagacttcc tttacgcaag ctgtggcaaa agcgctcggc
     3001 gtagaagacc atgtaacgag ccctacgttt gtgctcgcga aatcgtatgc gctgccggaa
     3061 gggctcgcct tcaaggagct cctccatatc gacacgtacc gtcttgaaga agggaaggat
     3121 ctcgtgccgc tcggattctt cgagtccatg gaagacacgg gcacgctcgt gttccttgag
     3181 tggccggaga tcgtcgcgga caagctgccg aagccggacg ttgccataac gatcgtggcg
     3241 aacgaggacg ggtcgcgtac catcacatat gcataagacc cgcctggtac tgcttgatgc
     3301 gcacgcgatt attcatcgtg catatcatgc actcccagat ttcacagcac cttccggaga
     3361 gccgacaggc gctctctacg ggctctcctc catgcttctt cgcatcgcga ccgagcttcg
     3421 tcctgattac gtagcggcct gctacgacct tccgaagcca acggtgcgtc acgaagcgta
     3481 tgcggactac aaggcaggac gcgcgcagac ggatgactcg ctcgcaaccc agctcaccac
     3541 ctcgcgccgc gtgttcgatg cgtttgcgat acctatatac gagaaggcag gattcgaggc
     3601 agatgacatt ctcggcacca tcgtcgagaa gctcaaggat aacgacgaag tcgagatcgt
     3661 tatcgcttcc ggcgacatgg acaccatgca gctcatctca ggcaagaagg tgcaggtata
     3721 taccctaaag aaaggactca acgacacgat cctctacgac gaggcggcgg ttacggagcg
     3781 ctacggattt ggtcctgagc gtatcgcgga tttcaagggc ctgcgcggcg acccttcgga
     3841 caacatcaag ggcgttcctg gcatcggcga gaagacgggt accgagctca tccagaagtt
     3901 cgggtcggtc gaagatatct atgactcgat tgagaaggac gagaatgcgg tgcttgcggc
     3961 aggcatcaag ccgcgcatcg taaaccttct gaaggagcac gaggaggatg cccgcttctc
     4021 gaagtccctc gctacgatcc gtctcgacgc gccgatctcg ttctcgttcc caaagaagtt
     4081 ctggaaggac tgcatcgatc aagcaaagtt gattgcactc tttgacgagc tcgggttccg
     4141 ctcgctgaag ccgcgcgtga agagcctact cacacaaggc ggaggggtag acgaagagga
     4201 cgaagaatct tttgaagaac ttcctgaaga ggaagatatc ccgaagaccc gccttgaaga
     4261 ggcaaaggtg atgctctggc tcctgtattc ggatttcacg aacccgtcgc tcgatgacat
     4321 tctcgcgtac accaaagcaa agtcctttga cgaagcatat gcggccctcg agaaggggat
     4381 cgctgagacc gggcgcctga aggaagtcta cgaggagatg gagcgtccct tgatcggcat
     4441 catagaggag atgcatcagt gtggcgtgcg tattgatacg gcggtgctcg aagacctgcg
     4501 caagaaatac cgaaaggagc tcaccgcgat tgagaagcgt atctacaaag cagcaggacg
     4561 cgagttcaat atcagctccc cgaagcagct tggcgaggtc ctgttcgacg agcttggtct
     4621 cgtggcaaag ggacagaaga agacttcgac cgggcagcgc tcgaccaaag agtctgagct
     4681 cgagaagatc aaggatcttc atccaatcgt tgcagacgtg ctcgagttcc gcggacttca
     4741 gaaactcctc tcaacctata tcgaagcgct tccgccgctc ctttgggacg acggcaggct
     4801 ccatgcgcag ttcctgcagt acggcgccgt tacggggcgc atggcgagcc agaacccagg
     4861 gcttcagaac atcccaatgc gtacagagcg cgggcgcgcg attcgtcacg cgttcgtgcc
     4921 gcgcgagggc ttcacgctcg tgggtctcga ttactcacag atcgagctcc gcatcgcggc
     4981 gatgctctca ggggaccaga agctcatcga catcttcaag tcaggccgtg acgtgcatca
     5041 ggaggttgcc gctgcagtct tcaacgtagc tcctgagaat gtggatcgcg agatgagacg
     5101 ccgcgcgaag atcatcaact tcggcatcct ctatggcatg ggcgtgaacg cacttaagac
     5161 ccagcttggt accacgacat ccgaagcgca cacgttctac gacgactact tcgagacctt
     5221 caagacactc gcacagtacc tcgagtccac gcgcggcttc gcgcgaaagc acggatacac
     5281 ggagaccttg tttggacgtc gccgtcagtt cccggaaatg aagtcgccgc ttccgtacgt
     5341 tcgggcgcag gcagagcgca tggctatcaa cgcgccgatc caaggaaccc aggcggatat
     5401 cacgaagcgc gcgatggtat ccgtagacag gcttcttaag gagatgggtg cgaatgagga
     5461 cgcgcacctg cttctgcagg tgcatgacga gctcgtatac gagatcagga acagcagggt
     5521 tgctgagctt gcagagaaga tcaggagcga gatggaacgc gcgcttcctg aggaatacac
     5581 cgaaggtgtg ccagtcatcg cggaagcgaa agctggctct gattggggca gcatggaagc
     5641 gctatgatct acctttttca tggaagcgac gcaggaaagg cacgcgctaa ggcgttccag
     5701 tgggtagcgg ccgcgcgcgc gaaggcgcca gacgcagcgt acatacggct cgaagggaag
     5761 gagataaccg aagcggcgct tacggaagcg ctctcaactc agggtctctt cttctcgaag
     5821 tctctcgtgc ttatagacga tccgttcgcc gctcaggaat cgggcgacat cgtactttcg
     5881 ctgattgatg cgcttgctgc gtctgagaat ccggttggta ttctcgcgcc gaagttgctt
     5941 gcggctcagg caaagaagct ggaagcgaag gctgcaaaga tattcaaaga agattcagca
     6001 gcaaaaccag cgcgagggtt caacagcgcg ctcgtgaatg cgctcggttc gaagaatgga
     6061 gtcgctcttt ggaaggagat agtgaaagcg ttccgttcgg gtgacgtgcc agagaccgtg
     6121 cacggtctgc ttcactggaa ggcgcgcgac ctgatgcaga agggaagtcc tacgtggacg
     6181 aaggaagaag cgagggcgct ttcagtgtcc cttatagagc ttgtggccga ttctcgttcg
     6241 ggagatttac cgctcggtcc tgcgcttgag cgattcgtac tctcattctc taactcttag
     6301 tccgcctacc tgggctcgaa ccagggaccg tctccttaaa agggagctgc tctaccgact
     6361 gagctatagg cggatgtggg cacgttatac cgctaatacg tatacaggtc taccgtcttc
     6421 ccgcttgcgt caagaagctt cacagaatca cgcgtcggtt tccagagctc gcgattctcg
     6481 tccagataga tgcgccacgt gtttccaacg aagttcgcgt cgcttcgatg atgctcgacg
     6541 catccgttat acgtgaggta ttcgtcgata agatcctcgc agcggcttga gacgcctgag
     6601 cgcgatggtg tagtgcagga cggtgtggac tgcatgcggt agtagcaggt gtcgtcacgc
     6661 aactcgttcc cggtgtagtg ctgggcgaac tcttcggacg cgagtggaca cgtaagcgga
     6721 agcgctggct gatacttttg gttgcgtgag aggtacccga tgcagcgatt ctccttgaag
     6781 gagacgccga gcggagactc tcccgtgaca acgagcgcgg tatcgcccgg ttgaagcaca
     6841 atacgatcgg tagggttcac gcgtcctgaa cgaggaagcg ccgctccttc cggaatgcgc
     6901 ccgctctttc cggacgctcc ggacacgagg cgccatccgg ttatgtcgat cggagcggtg
     6961 cctgtgtttt gaatagtgac gtattccaag tcagggtctg tagttcctgc gttattcacg
     7021 cccgagacac gcacttcgcc gcgaagcggg gaagggtcat tcgtgccctg aagcccggta
     7081 agcccgttcc caatatttgt gatgtctccc cagaagctgc ccccgtctcc atatccttgc
     7141 tggttcgtgt ccacgtccgt gatcggcgta atgtacgggc cagcgaatga gatggggcgg
     7201 ttcggcccgc cgctatagag ccatgcggca aagaagaaca cgaggacgcc aaggaagaag
     7261 actgcgtcac ttttcatgct cgtatagtac caagcgctag agaatcttcc ggagctcctc
     7321 gcgcactacc tgtgcatcgc tccacggcgt cttcacattg ttcgcttcca tgatgtacgg
     7381 atcggtaccc ttgccggtta tgaggaccgc gtctccggcc ttcgcgagcg agagtgcgcg
     7441 cgcgattgcc gcgcgacggt ccatgatgat ctctggtttc gtatccatgc ctgcagccat
     7501 cgcgtcgacg atagtgtgcg gatcctcgtc gtaaggatcc tcattcgcga ggataaccac
     7561 gtcgcagtac gtttctgcga tcttgcccat ctcaggacgc ttccaagtgt cgcgcccgcc
     7621 accggtgttc ccaagcacgc agatacggcg cgtagggtat gcctcgtaga gggccttcaa
     7681 ggaatccggc gtatgcgcgt aatcgacgat tgcctggaag ttctggccct cctcgatgcg
     7741 ctcggtgcgc cctgggatac gctcgagtgc tgcaagcccc tcggcgatct tcggaagggg
     7801 aacctcgagg aactcggctg ccttgattac cgcaagcgcg ttcatgatgc tgaagcttcc
     7861 cggaagctgg agcgtaaagc gaacgtcctt gtacgtgaac gaggcgcggc ctggagcgag
     7921 ttcaacgtcg ctcgcttcct cgtatgagaa cggacgcttc tcctcgaccg aaagcgcgag
     7981 atagggggcg cttcgtgggt cgtcgccgtt cgggattatc acgcgcgggc gcttcgggga
     8041 gcgggcgagc tccttgccga tctcgagttt cgcaagcgca tagttctcga atcctccatg
     8101 agactcgatg tgctccttct ggagattcgt gaagatgagt ccgttctgga agaggtagcg
     8161 atggcgatgt tggcgcgtgc tctcggaggt gatctcaacc acagcgtact tcgcacccgc
     8221 cttcacggct tttgcgagga acgcatggat aaaaccgcgt ccgggcatcg tcatcttgta
     8281 gaggtttggc tcagattcgt cgttaatcgc gaatctgata gtgccggcaa ccgcagccgg
     8341 gtaccctgcg tgacggagca ccgagaacag catgtcagac acggaagact ttcccttggt
     8401 gccggttacg ccgataaccg tgagtttttt tgcagggaat ccgtagcttg cggcaaggag
     8461 gaacgcgagc gtgcggtgat agaagggaag gagggggcgt actgcagaga tcggaaggat
     8521 ctttttgagg aactctatga gcgcgttcag tatcttgtac atagctattt cgagagcgct
     8581 gccttgaggc gcgcatcctt tccgagcaca gacgcgggaa cctttgagag agcggcacgt
     8641 gcctcgcgtt cggtgtaccc gagcgcaaca agcgtatcga acacctcaga gtcatctgtg
     8701 tcatgcgccc ttccggcgcc gatcttttct gaaagctcga ccaaaagttt ttccgcagct
     8761 ttcttcccaa ggccgatgac gcgggtgagg tagtcgaggt cgcgggtgcc gatggcgcct
     8821 tcgagcgctt cgcgcggcgc tttccggagg atgccgagcg cggtcttggg tcctacgcca
     8881 gggacaccca cgagaagctc gaagaatcgg cgctccgcct cgtccgggaa tccgtagagg
     8941 tccatgccgt cctgcttcac ggcaagatac gtcttaaggg ctatctcctg tccttcggca
     9001 taggcttccg gggcagacat atatatgaat gcgccccagc cggagatgtc gaggatgatg
     9061 cctcctggct ccgtgcctgc taccgtgccg cgtacctgcc tgatcatagg cgtatcgtag
     9121 catttcttgc gcatattccc ggaatgccgt agtatccgca ctacatggca atca
//
LOCUS       DASWXU010000049         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_294632, whole genome shotgun sequence.
ACCESSION   DASWXU010000049 DASWXU010000000
VERSION     DASWXU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294632"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1676
                     /gene="groL"
                     /locus_tag="VGE48_01720"
     CDS             48..1676
                     /gene="groL"
                     /locus_tag="VGE48_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447862.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY1042119.1"
                     /translation="MAKQIKFGEDARTAIHKGLKEAAEAVKVTLGPRGRNVVLEKSYG
                     GPRITNDGVSIAKEISFKDKFENMGAEMVKEVANKSNDVAGDGTTTTVVLLEAMVSDG
                     MKSLTKGASAMAIRAGMEQARDAAVKELKSMAKPVEGRTDIEQVASISAESEELGRII
                     AETVEKVGKNGVVTVEESQGMELSYEVVEGLQIDKGYASAYMVTNPERMEAEMRDAQI
                     LVTDKKISSVQEILPFLEKLAQSGRKDLVIVADDVEGEALTTFVLNKLRGTFNVLAVK
                     APGFGDRKKEMLADIAAVVGADVVSSDFGHTFENAELSVLGKASRVVAGKDTTVFVGG
                     KGRKSDIDARVGQLQALAEQTTSKFDKEKLEERIAKLSGGVAVIRVGAATETEMKYLK
                     DKIDDAVKATKASIEEGIVPGGGTALAKVARAIRVEDWEFDSQDERTGFAIVGKALEA
                     PLRQILKNAGKDDGSAIVERVQGAKGFAGYDALVGEEVEDMAEAGIIDPLKVTRSAVE
                     HAVSAAAILLTTEAAVADIPEPKPAQPDMGGMDF"
     gene            complement(1743..1883)
                     /locus_tag="VGE48_01725"
     CDS             complement(1743..1883)
                     /locus_tag="VGE48_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042120.1"
                     /translation="MHTMSLSAYELAALLGLALLVPKAREFCSGFCSGLFGWDDYDTS
                     ER"
     gene            1990..2550
                     /locus_tag="VGE48_01730"
     CDS             1990..2550
                     /locus_tag="VGE48_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY1042121.1"
                     /translation="MMTLWIVLGIVAVIVLWVVFAYNGFVSMRNRAKEAWSDIEVQMK
                     RRYDLIPNLIETVKGYAAHERQALESVTQARTQAMGAQTVTEHAEAENMLTGALKSLF
                     AVSEAYPDLKANTNFLELQRELSDTENKIMASRRFYNTTVQTLNTAVEQFPGNVIAKP
                     FGFSQMALFELSEGEEAAREPVKVSF"
BASE COUNT          622 a          680 c          845 g          504 t
ORIGIN      
        1 gatcctttcc taagtcttac ttccctctct aacttcccct ttactctatg gcaaagcaga
       61 tcaagttcgg tgaggacgcg cgtaccgcga tccacaaggg tcttaaggaa gcggctgaag
      121 ccgtgaaggt gaccctcggc cctcgcgggc gtaatgtggt gctcgagaag agctacggcg
      181 gtccccgcat tacgaacgat ggcgtgtcta tcgccaagga aatctccttc aaggacaagt
      241 tcgagaacat gggcgccgag atggttaagg aagtggcgaa caagagtaac gatgtagcgg
      301 gagacggcac cactactacc gtcgtacttc ttgaggcgat ggtaagtgac ggcatgaagt
      361 ccctcacaaa gggtgcgtcc gctatggcga tccgtgcggg catggagcag gcacgcgacg
      421 ctgcagtgaa ggaactcaaa tcgatggcaa agccggtaga gggtcgaacc gatatcgagc
      481 aggtagcatc gatctccgcg gagagcgaag agcttggtcg catcatcgct gagaccgtcg
      541 agaaggttgg aaagaacggc gttgtaacgg ttgaggaaag tcagggtatg gagctctcct
      601 acgaagtcgt tgagggtctt cagatcgaca agggctacgc gtcagcgtac atggtcacga
      661 acccagagcg catggaagcg gagatgcgcg acgcgcagat cctggtaacg gataagaaga
      721 tcagctcggt gcaggagata cttcccttcc ttgagaagct tgcgcagagc ggtcgtaagg
      781 acctcgtgat tgtcgcggat gatgtggaag gcgaggcgct taccaccttc gtcctcaaca
      841 agctccgagg cacgttcaac gtgcttgcgg taaaggcccc gggcttcggc gaccgcaaga
      901 aggagatgct cgcggatatc gcggcggtcg tcggcgccga cgttgtttcg agcgacttcg
      961 gacacacctt cgagaacgca gagctttccg tgcttggtaa ggcgtcgcgt gtggtggcag
     1021 gcaaggacac gacggtgttc gtgggcggca aaggacgaaa gagcgacatc gatgcgcgcg
     1081 tcgggcagct ccaggcgctt gccgagcaga ccacctcgaa gttcgataag gagaagctcg
     1141 aggagcgtat cgcaaaactc tctggcggcg ttgcggttat ccgcgtgggc gcggctaccg
     1201 agaccgagat gaagtacctc aaggataaga tcgacgacgc cgtgaaggcg acgaaggcct
     1261 cgatcgagga aggtatcgtg ccgggcggcg gcaccgcgct tgcgaaagtt gcgcgagcga
     1321 tccgcgtcga ggactgggag ttcgatagcc aggacgagcg cacaggcttt gcgattgtag
     1381 gcaaggcgct cgaggcgccg ctacgtcaga tcctcaagaa cgcaggtaag gacgatggtt
     1441 ccgctatcgt cgagcgtgtg cagggcgcta agggctttgc aggatatgac gcattggtag
     1501 gcgaggaagt ggaagatatg gcagaagccg gcatcatcga cccgttaaag gtaaccagaa
     1561 gcgcggtcga gcatgcggta tcggcagctg caattcttct caccactgag gctgccgtag
     1621 ctgatatccc agagccaaag ccagcgcagc cggatatggg cgggatggat ttctaaggaa
     1681 tcctaaaagg gagaaacgcc gcgactcgag agttgcggcg ttttcgtttg tggctctggt
     1741 cgtcagcgtt cgctggtgtc gtagtcatcc cagccgaaca ggccggaaca gaagcccgag
     1801 cagaattcgc gggccttggg aacgagcagg gccaggccga gcagagcggc cagttcgtaa
     1861 gcagataagc tcatggtgtg catcctccgt ttctcctccg accatactcc ctaagcgtga
     1921 catcggcaat gaaagggata tgaattccct tccttgcgtg aaagggcaga aattctacaa
     1981 tagatacata tgatgactct ttggatcgtg ctcggcattg ttgccgttat cgtgctttgg
     2041 gttgtgttcg catacaacgg attcgtctcg atgcgtaacc gcgcaaagga ggcatggtcc
     2101 gatatcgagg tgcagatgaa gcgtcgctac gacctcatcc cgaacctcat cgagacagtg
     2161 aagggatacg cagcgcatga gcgtcaggca ctcgagagcg tgacgcaggc acgcacgcaa
     2221 gccatgggcg cgcagaccgt aacggagcat gcggaagcag agaatatgct cacgggagca
     2281 ctcaagtcgc tctttgcggt ttccgaggca tacccggacc tcaaggcgaa tacgaacttc
     2341 cttgagcttc agcgcgagct ctctgacacg gagaacaaga tcatggcgag ccgccgtttc
     2401 tacaacacca cggttcagac cctcaatacc gccgttgagc agttcccggg gaacgtgatt
     2461 gcgaagccgt tcggcttctc gcagatggcg ctatttgagc tttccgaagg cgaggaagca
     2521 gcgcgcgagc cggtgaaggt ttcgttctag acagggcaga cgcagctgtg cgtcttagta
     2581 ggtaaggaaa caccacatgg ctaatctcta tcaggagcgc tcgaagaacg tgatgaagac
     2641 ctgggtcctc a
//
LOCUS       DASWXU010000050         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_815312, whole genome shotgun sequence.
ACCESSION   DASWXU010000050 DASWXU010000000
VERSION     DASWXU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815312"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /gene="gltX"
                     /locus_tag="VGE48_01735"
     CDS             <1..1296
                     /gene="gltX"
                     /locus_tag="VGE48_01735"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY1042122.1"
                     /translation="REVEGAIGHMMESLKWLGIQWDESIDVGGPYEPYLQSARVDMHR
                     EYARRLVDAGFAYPDPYTPEEVEAFRAQADAEKRPFLFRDHRPERFEEWDGTRPLRFK
                     VPTVKRFEWEDVTMGTLSAGEEALDDFILIKGDGYPTYNFAHVVDDIEMKVTHVMRGQ
                     EFVSSTPKFLSLYEALGEMPPVFVTLPPILGEGGNKKLSKRDGAKDILDYKREGYLPG
                     AMVNFLALLGWNPGGDRELFLSMHELIQAFDIDRIQRSGAQFDDEKLRHLNREHLRAI
                     PDEAFIRLGNFEGLDETVLLRAVPVLKERAYTLGEAEEMLHGELSCLFKTPAVSRDSL
                     VAKEPTDRPGATKQALTAVIEALEALPAQLPAEAVKMAIMPLADAEEAKGKGGRGAML
                     WPVRYALSGAERSPDPFTLISILGTETSLSRLRAALAIL"
     gene            1309..2649
                     /locus_tag="VGE48_01740"
     CDS             1309..2649
                     /locus_tag="VGE48_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1042123.1"
                     /translation="MRSFVLALFVLLTASFVGGLVPEGVYAQTESQIKGQIEEHEARI
                     KELEREIAEAEAQRAVYGAQAQTLQGLIRELDITRRQTNTKINLAENQISSTNLKLEE
                     LSYNIADTEELIALDQRTLAKSLRNMAQIGETSIVEQVFAAESLTEAWAAVDDAAALN
                     DALRANVANLTMARNELTGQRLDEDAKKQELTAFRADLSNQRQALDVQKSEKDRLLSQ
                     TKSQESVYQDLVTRKKAERALFEQALSALENSLASVGQGAAPQTQTGVLAWPYSASFA
                     ESCKGKAGALGNDKCITQYFGNTAFATANAQIYNGSGHNAIDIGMPIGTPVLAALSGT
                     VLATGNTDATPGCYSFGKWVMLKHANGLATLYSHLSTINVSSGQSVSTGQLVGNSGMT
                     GYATGPHLHFGVYASAGVQIMTLGQFRNTGGSGCSNATMPVAPKDAYLNPMSYL"
BASE COUNT          671 a          861 c          874 g          585 t
ORIGIN      
        1 agggaagtag aaggcgcgat cgggcacatg atggagtcgc tgaagtggct cgggatccag
       61 tgggacgaga gtattgatgt gggtggcccg tatgagccat accttcaatc cgcacgagtc
      121 gacatgcacc gcgagtacgc acgcaggctc gtggatgcgg gttttgcgta tccggacccg
      181 tatacgcctg aagaggtaga agcgttccgc gcgcaggcgg acgcggaaaa gcgtccgttc
      241 ctctttcgtg atcatcgtcc cgagagattc gaagaatggg atggcacacg tcctttacgc
      301 ttcaaggtcc ctaccgtgaa gcgctttgag tgggaagacg tcactatggg cacgctttct
      361 gcaggcgagg aggcgcttga cgatttcatc ctcatcaagg gcgacgggta tccgacgtac
      421 aacttcgcgc atgtcgtcga cgatatcgaa atgaaggtca cgcatgtgat gcgcggccag
      481 gagttcgtat cgtccacgcc gaaatttctc tccctatacg aggcgctcgg cgagatgcct
      541 ccggtgttcg tgactctgcc gccgatcctt ggcgagggcg gaaacaagaa gctctcgaag
      601 cgtgatggcg cgaaggacat cctcgactac aaacgcgagg gataccttcc aggcgccatg
      661 gttaacttcc tcgcgcttct cggatggaac ccgggaggtg accgcgagct cttcctttct
      721 atgcacgaac tgatccaagc gttcgatatc gatcgcattc agcgctcagg agcacagttc
      781 gatgacgaga agctgaggca cctcaaccgc gagcacctcc gtgctattcc ggatgaagcg
      841 ttcatccgac tcggaaactt cgagggtctt gatgagacgg ttctgcttcg cgcggtaccg
      901 gtcctcaagg agcgcgcgta cacactcgga gaggctgagg agatgctcca cggcgagctt
      961 tcgtgcctct ttaagacgcc tgccgtatcc cgggattcgc ttgttgccaa ggagcctaca
     1021 gaccgtccag gagccacaaa acaggccctt acggctgtta tagaggccct agaagccctt
     1081 ccggctcagc ttccggccga ggctgtgaag atggctatca tgccgcttgc ggacgctgag
     1141 gaagccaagg ggaagggagg ccgcggcgct atgctctggc cggtgcgcta cgcgctttcg
     1201 ggcgccgagc gctctccaga cccgttcacc ctgatatcca tactcggaac tgagacgtca
     1261 ctctcccgcc ttcgcgcggc tcttgctata ctctagggga tgcagcattt gcgttccttc
     1321 gttctcgcgc ttttcgtcct ccttaccgct tcattcgtag gcggtttggt gcctgagggc
     1381 gtatacgcgc agacggagtc gcagatcaag ggacagatcg aagaacacga ggcgcgtatc
     1441 aaggagctcg aacgcgagat cgctgaggcg gaagcgcaac gcgcggtata cggcgcgcaa
     1501 gcacagaccc tccagggcct tatccgcgag ctcgatatca cgcgccgcca gaccaatacg
     1561 aagatcaatc tcgcggagaa tcagatctca tccaccaacc tcaagctcga ggagctttca
     1621 tacaacatcg ctgataccga ggagcttata gcgctcgacc aacgcacgct cgcgaagtcc
     1681 ttgcgcaaca tggcgcagat tggcgagacc tcgatcgtcg agcaggtatt cgcggctgag
     1741 agccttacgg aagcatgggc tgctgttgat gatgcggcgg cgcttaacga tgcactacgc
     1801 gcgaacgtag cgaacctcac tatggcgcgc aacgagctta caggacagcg cctcgatgag
     1861 gatgcgaaga agcaggagct caccgcattc cgcgcggacc tatccaacca gcgtcaggct
     1921 ctggacgtcc agaagagcga gaaggaccgg ttgctttccc aaaccaagag ccaggagagc
     1981 gtgtatcagg acctcgtgac tcgtaagaag gcagagcgtg cgctcttcga acaggccctg
     2041 tccgcgcttg agaatagtct cgcatcagtg ggccagggcg cagcgcccca gacgcagacc
     2101 ggcgtgctcg cgtggccgta ttcggcatcg ttcgcggaat cctgcaaagg gaaggcgggt
     2161 gcgctcggaa acgacaagtg catcacgcag tacttcggca acaccgcgtt cgcgactgca
     2221 aatgcccaga tctataatgg ctcgggacac aacgccatcg acatcggcat gccaatcggc
     2281 acgccggtct tagcggcact ttcaggaacc gtgcttgcaa ctggaaacac ggacgcaacg
     2341 ccgggatgct attcgttcgg aaagtgggtg atgctgaagc acgcgaacgg tcttgcgacc
     2401 ctgtactcgc acctctccac catcaacgtg tcttcagggc aaagcgtctc gacaggacag
     2461 ctcgtaggaa actccggtat gaccggatac gcaaccggtc cgcacctcca cttcggcgtg
     2521 tacgcatctg ccggcgttca gatcatgacg ctcggacagt tccgtaacac cggcggctca
     2581 ggctgttcaa acgcaacaat gccggtcgca ccgaaagatg cgtatctgaa tccgatgtcg
     2641 tatctgtaag ttcgatccct agtctgccag caactgaagg cttattgaca gtcacatgaa
     2701 attttatata gtgcccacac cccagacggt agtctcaacc gagctggccg aagtcggcgt
     2761 tacggagaag cgattgcaag cgcagatccg aagaataatg ttgacgggca actaatgaga
     2821 ctagttcgat tcggaaagga atttcgccgg aaagaacgag acgctcttca gtcttcactg
     2881 cttgagcaaa agagagccct actggcaggt gtactgtagt cccaggccgg ttgaagggcc
     2941 gccgacacgc tcggcggccc tttctctatt tcagtacaaa agttccaaca t
//
LOCUS       DASWXU010000051         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_908673, whole genome shotgun sequence.
ACCESSION   DASWXU010000051 DASWXU010000000
VERSION     DASWXU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908673"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGE48_01745"
     CDS             <1..439
                     /locus_tag="VGE48_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY1042124.1"
                     /translation="IGKIAQFALVHDLVEVYAGDVVTSHSTFTEVDQKNKEEREAEAL
                     ARIKREFDDELPWIGDTIESYEKLNTPEARFVKTVDKAMPKIANILNKGITFKAQGHD
                     KESGEVFLSRQREKLSASYASDQPEAMALLEALGKEMIQEIYD"
     gene            432..764
                     /locus_tag="VGE48_01750"
     CDS             432..764
                     /locus_tag="VGE48_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqfO family protein"
                     /protein_id="HEY1042125.1"
                     /translation="MIDTEKRYKIVVYVPSSHADALRAAMGDAGAGRIGDYTHCMFTL
                     TGIGQFKPGEGADPYVGEVGKLNSVEEERIETVCEGGALEAVVRAIRETHPYEEPAFD
                     VYPIALIA"
     gene            822..1394
                     /locus_tag="VGE48_01755"
     CDS             822..1394
                     /locus_tag="VGE48_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEY1042126.1"
                     /translation="MKNQPSRPAQLLSFYKKHRRMPNITELMELFDLASRSSAFYAAE
                     RLVEEGYANKDRTGKLVPAPGLFRVPLVGHIRAGFAAPAEEELADTVTIGEYLLGDIN
                     ASYLLSVEGDSMEGAGIHAGDMVVFERTNDVSPGQIVVALTEDGYTLKYLRKRGKTFY
                     LEAANDAYPDIYPKEGQIIGKVTATFRKYG"
     gene            1387..2682
                     /locus_tag="VGE48_01760"
     CDS             1387..2682
                     /locus_tag="VGE48_01760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY1042127.1"
                     /translation="MDRSASYPRAIVHFDGDSFFASVEQAKDWRLRGKPVVTGGERGA
                     ATSLSIEAKRLGLHRGMPMQEIRRVCPEAVIVAGDYTAYSIYAKRMYAIARRFTPYVE
                     EYSIDECFADITGLDTVLGKSYEEIAESIKSALTQSLGITFGVGLAPSKTLAKVASKR
                     NKPDGFTALPRESIEEILADTEIYDVWGLGGALSSRLRGLGVVTALDYIRKDDGWLRE
                     HGFVKPARDTWLELKGYAISRVHTGAHALPHSVMKTRTFTPPSNDRAFILSQLSKNLE
                     GACAKIRKQGMKARALSFYLKTQEFTYHGVQLDLSVPLDMPPEILKEIDARLDAVYAR
                     GVLYRASGVTLRGLVTESATTPDLFGAYKEIDKRHDVFRAQDVVNRKHGRNTVFLASS
                     MRALTTPDASYRTKVAPGFVAKDRAKKKSLNIPYLGSAR"
     gene            complement(2718..2972)
                     /locus_tag="VGE48_01765"
     CDS             complement(2718..2972)
                     /locus_tag="VGE48_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042128.1"
                     /translation="MCKTHAPLVPELIEKEARRIVPLLDEMNDVSYGLVETLMGEFLK
                     AEEAKRTQRILYANVITKTLGLDFSLEDVIAILKRADELD"
     gene            3084..>4039
                     /locus_tag="VGE48_01770"
     CDS             3084..>4039
                     /locus_tag="VGE48_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY1042129.1"
                     /translation="MNDMRPEIGRALAGAGVLVVAASIALAISTIREGMEALPVAAVN
                     ASSVVSLTNEERAVKNLPALERNALLDLAARLKAEDMATKGYYAHVAPDGTTPMDFVN
                     RSGYRYRIIGENLVVERTSADQVVEAFMGSPGHRANILRKDFTEIGVGTAEGTHKGKD
                     AIFTVQIFAAPLATAQSSKEVVKPTSKVPEGSELPKAPVVTVPRPQAEQKPKTPAPVL
                     ATTTLSIAPLGEKVKEILKPLTMPLEATTTASTSSRPLEVPAYTPSFPITFSEPSVFE
                     DVSRLEVKTPSVAASSTWIEDISFKMKQILLTLHSLVSKFKS"
BASE COUNT          923 a         1039 c         1233 g          844 t
ORIGIN      
        1 tatcggcaag atcgcgcagt tcgcgctcgt gcatgatctg gtcgaggtgt acgcaggaga
       61 cgtagttacc tcgcattcga cgtttacgga agtggatcag aagaataagg aggagcggga
      121 agcggaggcg ctcgcgcgca ttaagcgcga gtttgatgac gaacttccgt ggatcgggga
      181 tacgatcgag tcctacgaga agcttaatac gccagaggcg cggttcgtga agacggttga
      241 taaggcgatg ccgaagatcg caaacatttt aaacaaaggc atcactttca aggcgcaggg
      301 acacgataag gaaagcggag aagtcttcct ctcaaggcag cgcgagaaac tgtctgcgtc
      361 ctatgcttcg gatcagccgg aagccatggc gctacttgag gcgcttggga aggagatgat
      421 acaagaaatt tatgattgat actgagaagc ggtacaagat cgtggtgtat gtcccgtcgt
      481 cacacgccga tgcgctccgt gctgccatgg gagacgctgg agcaggacgg atcggagact
      541 acacacactg catgttcacg ctcaccggaa ttgggcagtt caagcctggg gaaggcgcgg
      601 atccgtacgt gggcgaggtg ggtaagctca actctgtcga ggaggaacgt atcgaaacgg
      661 tgtgcgaagg aggggcgctt gaagctgtcg tgcgtgcgat tcgcgagacg catccgtacg
      721 aagagcctgc attcgatgtg tacccgatag cgcttatcgc gtagcgaggc gttctggggg
      781 tatctttcag aaccgaacat ttgttcggta tactggaagg gatgaaaaac cagccttccc
      841 gcccggcaca gctcctgtct ttttataaga agcatcggcg tatgccgaac ataaccgagc
      901 tcatggagct cttcgatctc gcgtccagga gctccgcatt ctatgccgca gagcgcctgg
      961 tagaggaggg gtatgcgaat aaggatcgga ccgggaagct cgtaccggct ccgggactat
     1021 tccgggtgcc tttggtgggg cacatccgcg ccggattcgc ggcgcccgct gaagaggagc
     1081 tcgccgacac cgtaaccatc ggcgaatacc tgctcgggga catcaatgcg agttacctgc
     1141 tttcggtaga gggagattcc atggaggggg ccgggataca tgcgggagat atggtggtgt
     1201 tcgaacgcac gaatgacgtc tcgcctgggc aaattgtggt cgcgctcacg gaagacggat
     1261 ataccctcaa gtacttaagg aagcgcggga agaccttcta tctcgaagcg gcgaacgatg
     1321 cgtatccgga catctatccc aaggaagggc agatcatcgg gaaggtgacc gccaccttcc
     1381 ggaagtatgg ataggagcgc gtcgtatccg agagccatcg tgcattttga tggcgactcg
     1441 ttcttcgcgt ccgttgagca ggcgaaggac tggcgtctcc ggggcaagcc ggtggttacg
     1501 ggcggagagc gaggtgcggc tacctcgctt tcgatagagg cgaagcgatt aggactacac
     1561 cgcgggatgc ccatgcagga gatccggaga gtgtgtccgg aagcagtgat cgttgcgggt
     1621 gactatacgg catactcgat ctacgcgaag cgcatgtatg cgatcgcgcg taggttcaca
     1681 ccttatgtag aggaatacag catcgacgaa tgcttcgccg atatcaccgg gctcgatact
     1741 gtgctcggga aatcatacga ggagatcgcg gagtctataa aaagtgcgct cacgcagtcg
     1801 ctcggtatca cgttcggcgt ggggctcgcg ccatcgaaga cactcgcaaa agtggcctcg
     1861 aagcggaata agcctgacgg gttcaccgcg cttccgcgag agtctattga agaaatactc
     1921 gctgataccg agatatacga tgtgtggggg ttgggcggcg cgttgtcttc gagattgcgt
     1981 gggctcggtg ttgtcaccgc gctcgattac atacgcaaag acgacggatg gcttcgcgag
     2041 cacgggttcg tgaagcccgc gcgcgatacg tggctcgagc ttaaggggta tgcgatatca
     2101 cgcgtgcaca ccggagcgca cgcgctcccg cactcggtca tgaagacgcg cacgttcacg
     2161 cctccctcaa acgaccgtgc gttcatcctc tcgcagctct cgaagaatct tgaaggcgca
     2221 tgcgcgaaga tcaggaagca aggtatgaaa gcccgagcac tttcttttta tctgaagaca
     2281 caagagttca cgtatcacgg cgtgcagcta gacctgtcgg tgccgcttga tatgccgccc
     2341 gagattctaa aggagatcga tgcgcgactc gatgcggtat atgcgcgtgg cgttctgtat
     2401 cgcgcgagcg gcgtgacgct tcgcggtctc gttaccgaga gcgcgacgac gcctgatctc
     2461 ttcggtgcgt ataaggagat cgacaagagg catgatgtgt tccgcgcaca ggatgtcgtg
     2521 aatcgaaagc atggacgtaa cactgtgttc ctcgcgtcga gcatgcgcgc gcttaccacc
     2581 ccagacgcga gctacagaac gaaagtcgct ccagggttcg tagcaaagga tagagcgaag
     2641 aagaagtcgc tcaacatccc gtacctcggg agcgcgcgct aaaagaagag cggcgccgga
     2701 gcgccgcaga aattctttta gtcgagctcg tccgctcgct tgaggatggc aataacgtct
     2761 tcgagagaaa agtcgagtcc gagagtcttc gtgatcacgt tggcatacag gatgcgctgc
     2821 gtgcgctttg cttcctccgc tttcaggaat tctcccatca gcgtttctac gagtccgtaa
     2881 ctcacgtcgt tcatctcgtc gaggaggggg acgattcttc gagcctcctt ctctatgagc
     2941 tcaggcacga ggggcgcatg cgttttgcac atggccgtac tccatgggaa cccgaaggtg
     3001 cggatccgct cgtagtatcc gggaaatatt cttaggtgca agtaccgttg catctgcgtg
     3061 attagggtgc acactattcg gtaatgaacg atatgcgacc ggagatcggc agggcgctcg
     3121 cgggagcggg cgtgcttgtg gtagccgctt ctatcgcgct cgcgatttcg acgattcgcg
     3181 aaggtatgga ggcgctaccg gttgccgccg tgaatgcctc aagtgtcgtt tcgcttacga
     3241 acgaggaacg ggcagtaaag aaccttccgg cgctcgagag gaacgcgctc cttgaccttg
     3301 cggcgcgcct caaggcggag gacatggcga ccaagggata ctacgcgcat gtcgctccag
     3361 atgggaccac cccgatggat ttcgtgaatc gttccgggta ccgctatcgc atcatcggcg
     3421 agaacctcgt ggtcgagcgc acgagcgcgg accaggtggt tgaggcgttc atgggttctc
     3481 ccggacaccg ggcaaatatc ctcagaaagg atttcacgga gataggtgtg ggtacggcag
     3541 agggaaccca taaaggtaag gatgccatct tcacggtcca gatattcgct gctcctttag
     3601 ctactgctca aagttctaaa gaagttgtga aaccaacatc aaaggttcct gaaggatcgg
     3661 agcttccgaa ggctcccgtt gttacggttc cacggcctca ggccgagcag aagcccaaga
     3721 cacccgcgcc ggtactcgct acgaccacgc tttccatagc gccgttaggc gaaaaagtta
     3781 aagaaatcct aaagccgctc acgatgccgc ttgaggcaac taccactgcg agcacttcat
     3841 caagacctct tgaggttccg gcgtatacgc catccttccc gattacgttc tctgagccat
     3901 cggtttttga agatgtttcc cggttagaag tgaagacacc atccgtggca gcaagttcca
     3961 cttggataga agatattagt tttaaaatga aacaaatcct ccttacgctc cattcgttag
     4021 tgagtaagtt caagtccta
//
LOCUS       DASWXU010000052        13807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_357288, whole genome shotgun sequence.
ACCESSION   DASWXU010000052 DASWXU010000000
VERSION     DASWXU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357288"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VGE48_01775"
     CDS             <1..841
                     /locus_tag="VGE48_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEY1042130.1"
                     /translation="FYVRHTVGGFDLETWAVSDPYRLDVLMWVGLAFLALVFLFGGLQ
                     GIRGLASLAGSLVLIFYLLLPGIYSGGSPILISLGVAGLIIVVGSYVTHGVNKTTTAA
                     MLGMLATILVTGLATYFVIGMASLSGFTSEENAYLNFATSGSLDMVGLLFGGIMIGLL
                     GVLYDSAIGQAVAVEELYTAGTNYTRKQVYLRALRIGREHIGALVNTLAIAYVGASLP
                     LLLLLKQSTASPLYLLNSEVFATEIIRILMGSIGVILAVPITTLIATSLLKGTKPSGI
                     HAH"
     gene            909..1559
                     /locus_tag="VGE48_01780"
     CDS             909..1559
                     /locus_tag="VGE48_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY1042131.1"
                     /translation="MSLSSYAKKHPLAIYGLVWLLSLFIVSLELIGSGVSGSQALLAD
                     VGHVASDTLLALVPLAALLFVRLGWNYRKVAFVSSLAAVAILLWVGVHVGGEAWEALM
                     GEEHHDHEVDGMFLFLFSGVAAIANLIQHRLLSRIDPEHHHGAHQGLHFHVLMDLVKN
                     VALPMLGILIALEFIPDHADLWAALVIGALLIIRALVLLYTTLFQEKRMHAGHDHA"
     gene            1546..1962
                     /locus_tag="VGE48_01785"
     CDS             1546..1962
                     /locus_tag="VGE48_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY1042132.1"
                     /translation="MTTLNHEQILRDAGLRVTPGRIALLHALAKESKPVPVEKLAKSL
                     KGELNQVTLYRALEAFEGANIVTRAGLGHEHAHYELLLGRPHHHHAVCRTCGHVEDIE
                     VPHGPSPEKDAERRAKGFASIDRYSLEFFGTCHSCV"
     gene            2078..2746
                     /locus_tag="VGE48_01790"
     CDS             2078..2746
                     /locus_tag="VGE48_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1042133.1"
                     /translation="MKILVVEDEERISRFLKESLEAEFFAVDVAEDGEQGSYFARTND
                     YDLVVLDNMLPKKDGLQVCKEIRASGKTMPILVVSVKTGTFDKVDLLDAGADDYLAKP
                     FVLEELLARVRALMRRPTVIESDVLRVEDIELNAKTGTVRRGDRNISLTRKEFMLLQY
                     LMRNEGTVLSRGMILEHVWDMSVDIFSNTIESHILSLRKKLGDTKDDRIIQTISGRGY
                     RMGA"
     gene            2828..3499
                     /locus_tag="VGE48_01795"
     CDS             2828..3499
                     /locus_tag="VGE48_01795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1042134.1"
                     /translation="MTDEPGQTKAAYDRMGAAYDALSEDFHQEVAYPALLTLLERGLG
                     SLQNRSILDVGCGSGRLVRRLQQKGANACGIDLSEGMASRGRNLGLPITTGSMLELPY
                     KDDSFDAVVSYHSFNYVPFESQRHAIEEQCRVLKIGGTLLLSLFHTAVSVRVPLSVSS
                     EGEELRLYLRTLDEMRDLLNSCHCIAHEAPIATPAQARLVPESSRAYVTERPYALFIV
                     AHRIV"
     gene            3642..4616
                     /locus_tag="VGE48_01800"
     CDS             3642..4616
                     /locus_tag="VGE48_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY1042135.1"
                     /translation="MRTFTGISLPKGADHNTVYRLLPNAPDDMYYRERTNRNIGWLTE
                     AEQQILRNSVIGIAGCGGMGGMLASLFCRMGIGEVRIADSEVFDLSNVNRQFGASRMS
                     IGQSKALETARLTRAICDDNTLVVFPQGIIEESVQDFARGCHIILDEIEFWAVGSRIL
                     LHQAARALGVPLLNCNAVGFGSRLFRFDPEGFTMEELLGFTYERAKLLQGRIQSGAAT
                     AEEIALVMERMFQGLVPELPEYACEGAGYSTVSAVRARLLTEQRASIVSSTPLLASGF
                     LATHTVFELLRKSAIPRVYQRPPAAPGYLIIDAGFHEARVVTRKGSDL"
     gene            4613..5194
                     /locus_tag="VGE48_01805"
     CDS             4613..5194
                     /locus_tag="VGE48_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042136.1"
                     /translation="MSGELSVFLAEHEEDICEVHSLVERTYLASYGTVPRPAQAYGAV
                     RRGSEVVACLGFDHAEPDGRFAIERIYSLDRTQYPVPVTSDTTAQFGRLVSSEPCLGR
                     LAVHTGIWFALRLGKRLGIVEHNDVIHRVTLGFGLVLLDIPFWEINLGETLPQDRGYY
                     EKREGKPYLMDLEKSLERTAARLSPDVLALFKE"
     gene            5262..7226
                     /locus_tag="VGE48_01810"
     CDS             5262..7226
                     /locus_tag="VGE48_01810"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1042137.1"
                     /translation="MAPSLTPDILLLAIVAAANFAIGVYVFLQRPEDRARQAFFLFVT
                     GGALWVGGFVLLSLTHNFFFDIVTNYGGLLLIAGTLWFAQNFPHPIKLPVRSWLLYFP
                     FLIIAVGVIPQQLIVLDIQPLPDGGFVPIVGPLLPLWSLIAVTFTITATSILVYRFRR
                     STGRERLQLGYVFAGVSTFALSAVVFDMVLPVFGITEFKSIGPLTSIVFVAATAYAIA
                     RHQLMDIRIVIQRGFTYSILLGLIIVCYLSVLSVLSALFPRDASGTTLAASLVTITLG
                     IIGVPYIERWFRRATDRIFFKGTYEYATALETLSAVLHANVELNELVRESEQALERIL
                     RATSVHITLGTSESGTHKNAALLVPIGLDGEDIGCIQVEAKRSGDPYTSEDLQLLKTF
                     AYQAATALSRAQLYADVKEHAKDLEAKVEERTRELSRAQARERQMLNDLSHNLQTPLT
                     VLQTRLDRLKPSLKDHAEVHALEQSLESFSGFVYELLSLARLEGGRRIARVPVDVSAL
                     VRELSDEISVIAENRGIQVESRIEEDLYVKGDERKLREALMNLASNALKYQKSDGDRR
                     IGFALMRESDTAVLEISDTGTGIAPQDLPRVFERFYRGSGLPSEMRGTGLGLAIVKRI
                     IEQHKGTIVAQSTHNEGTTITIHLPLVSEH"
     gene            7487..7690
                     /locus_tag="VGE48_01815"
     CDS             7487..7690
                     /locus_tag="VGE48_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEY1042138.1"
                     /translation="MSALKPVAFILVIVGALNWGLVGLGGFMGSEWNLVNMLFGAWPM
                     VEWIIYVLVGLSAIVLLATKRRM"
     gene            complement(7742..9433)
                     /locus_tag="VGE48_01820"
     CDS             complement(7742..9433)
                     /locus_tag="VGE48_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY1042139.1"
                     /translation="MHRNTAFVAAMLLSLFAIPSTFVHAAYGGLDTAFDTDGSVFFTA
                     AQVGLPDSEPEVTGLLKQPDGKIIFSANSFVGPDPYPSSIVRVTATGALDTSFGNGGS
                     FTFDINDLAVVRDIALQGSQILFVGNYGNTSDSYGGFIGRLSSAGVLDPSFGNHYSES
                     GTTHDGYALLIGDQYSFLAVEVDSSNRIIVLGEDNDQGDSFLARYTQAGVLDPSFGIN
                     GMTTFANTNLTDMVMREDGGFLLGGYVGDDVAVYAVEADGDLDTSFASNGIFTETLPG
                     VDYARSEHLSIASDGSIMLGGYVSDNFDRHAFYVRINEDGEDFDIVTVDDSAVNDQIQ
                     GMVADGDGAVVVGGILFGDQGVLRAYDLEGDPITSFGTNGVIDLAMDVLGEPHLYYGD
                     ERITLAVGQNGTPGVQLFQYISTEPEPAPVVVDEAPSRRTGTSIQSRVKALIRMGNTT
                     RAEELMRQYPRLFPKEDVRTSDTVVPVLPVRDLMTGMSGEDVRALQKLLNANGFIVAN
                     TGPGSPGSETDLFGALTHAALAGYQAAHGIAPAAGYLGPVTREHMKIAGMNGIWW"
     gene            complement(9522..9592)
                     /locus_tag="VGE48_01825"
     tRNA            complement(9522..9592)
                     /locus_tag="VGE48_01825"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9558..9560),aa:Met,seq:cat)
     gene            complement(9633..10229)
                     /locus_tag="VGE48_01830"
     CDS             complement(9633..10229)
                     /locus_tag="VGE48_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042140.1"
                     /translation="MGTTTAPTVHFLNVEYFFRLFYELITSPRGIVFEAGGFMALASQ
                     IWLLVTALSLVISIALLIVFVNAVIRYHQIEHEDEVRHATLHAEHAEEKRDHSRWAHV
                     RELIESPHESDWRQAIIEADIMLDDLLSQLGYPGDSVGEKLKSVDPSRFHTLQNAWDA
                     HKVRNEIAHSGSAYQLEEHMAHRTIALYETVMREHGEI"
     gene            complement(10238..11671)
                     /gene="gatA"
                     /locus_tag="VGE48_01835"
     CDS             complement(10238..11671)
                     /gene="gatA"
                     /locus_tag="VGE48_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY1042141.1"
                     /translation="MTKPLNELSITEAAKGLRAKEFSVRELWDACATAAREKNPELNA
                     YLELFDADEDVIAAAQKRIDEEGERAPMLCGIPLAMKDNILIEGRIASAASRMLENHR
                     AVYDATITKKLKDAGALFIGRTNMDEFAMGSSTENSAYGVARNPIDTTRTAGGSSGGS
                     ASAICADMALGGLGTDTGGSIRQPAAHTGLIGLKPTYGAISRFGLIALGSSLDQAGPI
                     AKTVADAKVLFDAMRGKDANDATTIDEHTYPKPTEHTALRIGVPEHLLKEGLDEDVAA
                     NFRKTLDALKAKGHTLVEVSLPTSGYALPAYYVVMPAEASTNLARFDGIRYGLAERGE
                     TLLEDYVKSKTAGFGRETVRRILLGTFVLSSGYIDAYYRKAERTRDVLRAEYEEAFKS
                     CDVIAFPTTPSPAFRFGEKSDPVSMYLEDVFTVTADLTGMPALSVPMGDVTRDTVSLP
                     TGIHFTAPLGADDVLFRIGADVTGETL"
     gene            complement(11664..11960)
                     /locus_tag="VGE48_01840"
     CDS             complement(11664..11960)
                     /locus_tag="VGE48_01840"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY1042142.1"
                     /translation="MSKASAEDVKRLAALARVEVPEADLPRFAAEFDSILEYVGKLEE
                     LDLPETGRNLSSVRNVLRADENPSAPGTWTDAITAQFPDREGDSLKVKQIISHD"
     gene            complement(11977..>13807)
                     /gene="ligA"
                     /locus_tag="VGE48_01845"
     CDS             complement(11977..>13807)
                     /gene="ligA"
                     /locus_tag="VGE48_01845"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY1042143.1"
                     /translation="TVAGKALPGLKKVHHQVPQWSLDDAFDEAELRAFDERVRKGLAK
                     SFGGSTEPAYTCELKIDGAHIILTYEKGELVLAATRGDGVVGEDVTHTASTIKSIPKH
                     LLRPVDLIVEGEVYMSRKGFEALNRAQEKAGKPLFANPRNIVAGSLRQLDASVAAARP
                     LGAFLYDIEGLSEEIPRTQAEELSYLRSLGLPVNPHEKHASSVEEILAFWKKWQGSAR
                     EKEDYQLDGVVVKVDSRAHQEALGHTGKGPRYAIALKFPAEQVTTIIEDISLQVGRTG
                     VLTPVAHLKPVAVAGTIVARATLHNEDFIRDKDIRVGDTVILQKAGDIIPEIVQVLPE
                     FRTGKEKKWKFPASSPLCGGDGTIERVPGTAARRCAVAGSFDQQLRKLSHFAGKSALD
                     IDGLGEKTVKLLMEHELVSNMDDFFDLTKDELLQLPGFKEKSAQNLIDALADKKQIPL
                     DRLLVGLSIPHVGEETARVIAIEFSTLDALRAAKASAFSDIDGVGEVVAEALSDWFAD
                     PENQSLLDRLSAHLSVSAIEREGTGGPLEGMSVVITGSFESFSREEGEAMVRRAGGKT
                     ASSVSKKTGFVVAGEKPGSKLAKAEELGVSVIDEAEFLRRVGS"
BASE COUNT         2984 a         3943 c         3587 g         3293 t
ORIGIN      
        1 cttctacgtg cgtcacacgg ttggcggatt tgatcttgag acctgggcgg tctcagaccc
       61 gtatcgtctc gacgtgctca tgtgggtagg tctcgcgttc cttgcgctcg tgttcctttt
      121 cggaggactc caaggaatcc gcggactcgc ctcccttgca ggaagcctcg tgctcatctt
      181 ctatctactc ctacccggta tctatagcgg cggctctccg atcctcattt cgcttggcgt
      241 agcaggactc atcattgtcg tgggatctta tgtaacgcat ggcgtgaaca agacaacaac
      301 ggccgcgatg ctcggcatgc tcgcgaccat actcgttacc ggccttgcga cctatttcgt
      361 aatcggtatg gcctccctct caggattcac ctccgaagag aatgcatacc tgaacttcgc
      421 cacaagcggc tcgctcgaca tggtaggcct cctgttcggc ggcatcatga tcggtcttct
      481 cggtgtcctc tatgacagcg cgattggtca ggcagttgcc gtagaagagc tgtataccgc
      541 aggcacgaat tacacccgga agcaggtgta cttacgggcg cttcgcatcg gccgtgagca
      601 tatcggcgcc ttggtgaata cgctcgccat cgcctatgtg ggcgcgtcgc tcccgcttct
      661 acttcttttg aagcaatcta cggcgagtcc cctctacctt cttaatagcg aggtcttcgc
      721 aacggagata atccgcatcc tcatgggaag catcggtgtg attctcgccg tgccgatcac
      781 cacgctcatc gccacgtcgc ttcttaaggg aacaaagcct tctggtatcc acgcgcacta
      841 acgcttgact ctctcccctt ctggtataga gtgctggtat tattaatgca aataaattgc
      901 atttatccat gtctctctct tcgtacgcaa aaaagcatcc gcttgcaata tacgggctcg
      961 tctggctact gagcctcttc atcgtatcgc ttgaactcat cggctcaggc gtgtccggta
     1021 gccaggcact ccttgcggac gtaggacatg tcgcgagcga taccctgctc gcgctcgtac
     1081 cactcgccgc gctcctcttc gtgcgtcttg gctggaacta tcgcaaggtc gcattcgtaa
     1141 gtagcctcgc tgcggtagcg attctcctat gggtaggcgt gcatgtgggc ggcgaagctt
     1201 gggaggcgct catgggagag gagcatcacg accatgaagt agatggtatg ttcctgttcc
     1261 tcttctcggg cgtagcggca attgcaaatc tcattcagca ccgccttctc tctcgcatcg
     1321 atcctgaaca tcaccatggc gcgcatcagg gtctccattt ccatgtgctc atggacctcg
     1381 tgaagaacgt ggcgctgccg atgctcggta tcctaattgc gcttgagttc atccctgacc
     1441 acgcagacct ctgggccgcc cttgtcatcg gcgcgcttct catcatccgt gcactcgtgc
     1501 ttctgtatac gaccctcttc caggagaagc gtatgcacgc cggccatgac cacgcttaat
     1561 cacgagcaga ttctgcgcga cgcaggactc cgtgttaccc cgggccgtat tgccctgctt
     1621 catgcgctcg caaaagaatc aaagcccgta ccggttgaga agcttgccaa gtctctcaag
     1681 ggagaactca accaggtaac cctgtatcga gcactcgagg cgtttgaagg agcaaacatc
     1741 gtaactcgcg cgggtctcgg gcatgagcac gcacactacg aactccttct tggtcgtccg
     1801 catcatcatc atgctgtgtg ccgaacctgc ggacatgtcg aagacatcga agtgccgcat
     1861 ggaccctccc ccgagaagga cgccgagagg cgcgctaagg gattcgcttc catagaccgc
     1921 tattcgcttg agtttttcgg cacctgtcac tcgtgcgtct agcgctatgc acacaggcat
     1981 tgacatagtc tctatgtcgc gttcctggtg ctagaatcta gataagaaat atgaatcctt
     2041 cattcgggaa gtttgtgtct atcccgacat ctcgcgtatg aagatactcg ttgttgagga
     2101 cgaggagcgc atctctcgct tcctgaaaga atcccttgag gctgagttct ttgcggtcga
     2161 cgtcgccgag gacggcgaac agggctcgta cttcgctcga actaatgact atgaccttgt
     2221 cgtgctcgat aacatgcttc cgaagaagga cgggcttcag gtatgcaagg agatccgtgc
     2281 gagcggcaag accatgccga tactcgtggt atccgtaaaa accggaacat tcgacaaggt
     2341 ggatctgctt gacgctggtg ctgacgatta ccttgccaag ccctttgtgc ttgaggagct
     2401 tttagcgaga gtacgcgcgc tcatgagacg acctactgtc atcgagagtg atgttctgcg
     2461 cgtcgaggat atagaactta atgctaagac gggtacggta cgtcgcggtg accgtaatat
     2521 ctcacttacc cgcaaggagt tcatgctctt gcaatacctc atgcggaacg agggtacggt
     2581 gctctcacgt ggaatgatac tagagcacgt gtgggacatg agcgtagata tcttctcgaa
     2641 caccatcgag tcccacatcc tctcgcttcg gaagaagctt ggagatacta aagacgaccg
     2701 cataattcag accatctctg gacgcggata ccggatgggc gcatagtcgc tatctttttt
     2761 tcatattttc gttatctcct tctgagagag tgtgtgaaga ctggtcattt acacacggga
     2821 gatttatatg accgatgaac ccggtcaaac aaaagctgca tatgatcgca tgggcgcagc
     2881 atacgatgca ctttccgagg acttccatca ggaagtcgcc tatcctgcgc tactgacact
     2941 acttgagcga ggtctcggct cgcttcaaaa ccgctcgata ctggacgtgg ggtgcggttc
     3001 tggaagactc gttcggcgcc ttcagcaaaa aggcgcaaat gcatgcggta tcgacctctc
     3061 agagggcatg gcatcgcgtg gcaggaactt gggacttccg atcactaccg gctcaatgct
     3121 cgaacttccc tacaaagacg attcgttcga tgcggtggtt tcgtaccact cattcaatta
     3181 cgttccgttt gaaagccaaa ggcacgcaat tgaagagcag tgccgggtgc tgaaaattgg
     3241 aggtaccctg ctgctttccc tcttccacac cgcagtatcg gtacgtgtac ctctaagtgt
     3301 ttcgagtgaa ggcgaagagc tcaggctcta tctccgcaca ctcgatgaga tgcgcgacct
     3361 actcaattcc tgtcactgca ttgcgcatga agctccgatt gcgacacccg cacaggctag
     3421 gctcgttccc gaatcgtccc gcgcgtatgt taccgaacgt ccctatgcgc tgttcattgt
     3481 tgcgcatcgt atcgtctgaa cttcttcgac atcccgttcc cgcgggatgt cttcttatgc
     3541 aaaattcatc tttagattac tttttcttga ttacgacgct acatcggtct gcgtactctt
     3601 cgctcagcgt gttctttgaa gcatcagaaa aggagtatgc gatgcgcacg ttcactggca
     3661 tctcactgcc caaaggagca gaccataaca cggtctatcg tcttcttccc aatgctccag
     3721 acgatatgta ttatcgcgag cgcactaacc ggaatatcgg ttggcttact gaagccgaac
     3781 aacagatcct tcggaattca gtcattggca ttgcaggctg cggcggcatg ggaggcatgc
     3841 tggcctcact cttttgtcga atgggcatcg gcgaagttcg catcgcggat tctgaggttt
     3901 tcgacctttc gaacgtgaac cggcagttcg gtgcgagccg gatgagtatc ggacaatcaa
     3961 aagcactcga gactgcccgg ctgacccgtg ccatatgcga cgacaatacg cttgtggtgt
     4021 tccctcaggg cattatcgag gaatctgtac aggatttcgc ccgaggatgc catatcatcc
     4081 tcgacgagat cgagttctgg gcggtgggtt cccgcatcct gctgcaccag gccgctcgcg
     4141 cccttggtgt accgcttctc aattgcaatg ctgtcggatt tggatcgcgg ctcttccgct
     4201 tcgatcccga gggattcacc atggaggaac ttctcggctt cacctacgaa cgcgcgaaac
     4261 tcttacaggg gcgcatccag tctggggcgg caacggctga ggagatagcg ctcgtaatgg
     4321 agcgcatgtt ccaaggcctg gtaccggagc tccctgaata tgcgtgcgag ggcgctggct
     4381 acagcacggt gtctgcggta cgcgcgcgac ttctaactga gcagcgtgca tccatcgtgt
     4441 cgtccacccc gctccttgcg tctgggttcc tcgcaacgca taccgtcttc gagctactcc
     4501 gcaaaagcgc tattccgcgg gtatatcaga gaccgcctgc ggcacccggc tacctgatca
     4561 tcgacgcagg cttccatgaa gcccgggtcg ttacccggaa aggatctgac ctatgagcgg
     4621 agaattgagc gtcttcttgg cagaacacga ggaagacatc tgtgaggtgc acagccttgt
     4681 agaacgaacc tacctcgctt cctatggcac cgtgcccaga ccagcacaag cgtatggtgc
     4741 ggttcgtcgc ggaagcgagg tagtggcttg tcttggattc gaccatgcag aaccagatgg
     4801 ccgattcgcg atagagcgta tctactctct ggaccgtacc cagtaccctg tacccgttac
     4861 gagcgacact actgcccagt tcggcagact agtatccagc gagccgtgcc tcggcagact
     4921 cgcggttcat acaggcatat ggttcgctct aaggttaggc aagcggctgg gcatcgtcga
     4981 acacaacgac gtcatccatc gcgtaacctt gggttttggc cttgttctcc ttgatatccc
     5041 attttgggag atcaaccttg gagagacgct tcctcaggac cgagggtact acgaaaagcg
     5101 tgagggtaag ccatacctca tggatctcga gaaatcccta gagcgcacag cggcaagact
     5161 ttcaccggat gttctcgcgc tcttcaagga ataagactct ctaaattacc agactgtggt
     5221 gcgggctgcc tcgcatgccc gcacttttat atacttactc catggcaccg agcctcacac
     5281 ccgacatcct actgcttgcc atcgttgcgg ctgctaactt cgctattggg gtatatgtat
     5341 tcctgcaaag gccagaggat cgcgctcgcc aggctttctt cctctttgtt accgggggtg
     5401 cgctgtgggt aggcggattc gtactgctct cgcttacgca caatttcttc ttcgacatcg
     5461 tgacgaacta cggaggattg cttctcatcg ccggtacgct ttggtttgct cagaactttc
     5521 ctcaccccat caagcttccg gtgcgttcct ggctgctcta ctttcccttc ctcataattg
     5581 cagtaggggt cataccacag cagctcatcg tgcttgatat acaacccctt cctgacggag
     5641 ggtttgttcc gattgttggg ccactgcttc ctctctggag ccttatcgct gttaccttca
     5701 ccattaccgc aacctcaatc cttgtctatc ggttcagaag atcgaccgga cgcgagcgtt
     5761 tgcagctcgg atatgtattc gctggcgtaa gtacgtttgc gctctccgcc gttgtatttg
     5821 atatggtgct gcccgtgttc ggtattacag agttcaaaag tattggtcca ctcacctcta
     5881 tcgtcttcgt agcagccacc gcgtatgcga tagctcgaca tcagctcatg gatattcgca
     5941 tcgttattca gcgaggtttt acgtacagta tcctgctcgg tctcattatt gtctgctacc
     6001 tgagtgtact ctctgtgctt agtgcgctct tcccgcgcga tgcgagcggc accacccttg
     6061 ctgcgtcact cgtaaccata acgctcggga ttatcggcgt gccatacatc gagcgctggt
     6121 tcaggagagc cacagatcgt atcttcttca agggaacgta cgagtacgcg actgcacttg
     6181 agaccctgtc tgcagtactc catgcgaacg ttgaactcaa tgagctcgta cgcgaatcgg
     6241 agcaggcact tgagcgcatt ctgagagcaa cgtcggtgca cattaccctt ggtacaagcg
     6301 agtcaggtac gcataagaat gcggcgcttc tcgtacctat aggtctcgat ggcgaagata
     6361 tcggctgcat tcaggttgaa gcgaaacggt ccggcgaccc gtatacttca gaggacctac
     6421 aacttctaaa gaccttcgca tatcaggcag ctactgcgct ctcacgggct cagctttatg
     6481 cagacgtaaa ggaacacgcg aaggatctcg aggcaaaagt tgaagagcgc acccgcgagc
     6541 tctcgcgagc ccaggcacgc gagcgccaga tgctgaatga cctctcgcac aatctccaaa
     6601 caccgctcac cgtgcttcag accagactcg atcgactgaa accgtccctt aaggatcacg
     6661 cggaagttca cgcactcgag caatcccttg agagtttttc gggattcgtc tatgagctac
     6721 tgtcgcttgc acgtcttgaa ggtggcaggc gcatagcgcg agttccggtt gatgtgagcg
     6781 cgcttgtgcg cgagctttct gacgagatta gcgttattgc tgagaatcgt gggatacagg
     6841 ttgaaagtcg tatagaagag gacctgtacg ttaagggcga cgaaagaaaa cttcgcgaag
     6901 cgctcatgaa tttagcaagc aacgcgctta agtatcagaa gtctgatggt gaccggagaa
     6961 taggttttgc gctcatgcga gagagcgata ctgcggtgct tgagattagt gacacaggca
     7021 caggcatagc tcctcaggat ctgccgcgcg tattcgagcg cttttatcga ggaagcggtc
     7081 ttccaagcga gatgcgcggt acaggtctcg gactagcgat cgtgaagcgt atcattgagc
     7141 agcacaaagg aacgattgtg gcacaaagca cacacaacga aggaacgact atcaccattc
     7201 atctgccact cgtctctgaa cactaatgtg cgtatcccga taccggacca gcttatccga
     7261 ccttagatat aagaccggtg ttgcgcaagc tggcccgatg tcgagaggcg caatgcgtat
     7321 atctctggcc agagtctacg cgagcgccct aaaggagttc taaaatttct atagagcgaa
     7381 gataaagtgg tagttatgca ccaccctccc taaaagcggg tacccagggt ccatacttat
     7441 agagtgtcgc gattatccgg ttccttaaga gttacttcgt atcggtatgt ctgcacttaa
     7501 gcctgtcgcg ttcatcctcg ttatcgtcgg tgccctcaac tggggacttg tcggcctagg
     7561 cggattcatg ggttccgagt ggaatctcgt gaacatgctg tttggcgcat ggccaatggt
     7621 tgagtggatt atttatgtcc tcgtcggcct ctcggcaatc gtccttcttg ctaccaagcg
     7681 caggatgtag cgtcactccc aaagagaagg cgccgcttca gtgcggcgct ttctctattc
     7741 cctaccacca gataccgttc atcccggcta tcttcatatg ctctctcgta accggaccaa
     7801 gataccctgc tgcgggtgca ataccgtgcg ccgcctggta tcccgcgagt gcagcgtgcg
     7861 tgagcgcacc aaagagatca gtctcactcc ctggagaacc agggcccgta ttcgcaacta
     7921 taaatccatt tgcattcaag agcttctgga gcgcccgcac gtcctctcca gacattcccg
     7981 tcataaggtc cctgaccggg agcacaggca ctacggtgtc agatgtgcgt acgtcctcct
     8041 tcgggaaaag cctcgggtac tgacgcatga gctcctctgc acgtgtagta tttcccatcc
     8101 tgatgagtgc tttcacgcga gactggatac tggtgccagt ccgtcgactc ggtgcctcat
     8161 cgacaaccac cggagcgggt tccggttccg tggagatgta ctggaaaagc tgcacgccgg
     8221 gagtaccgtt ctgacctacc gcaagcgtga tccgttcatc cccgtaatag agatgcggct
     8281 ctccgagtac atccatagcg aggtctatca ccccgttcgt cccgaagctc gtaatgggat
     8341 caccttccag gtcatatgcg cgaagcacgc cctgatcccc gaaaagaatg cctcctacca
     8401 cgaccgcacc atcaccatct gcaaccatgc cttggatctg atcattcacg gccgaatcgt
     8461 ccacggtaac aatgtcgaag tcctccccat cctcatttat acgcacatag aacgcatggc
     8521 gatcgaagtt gtcgcttacg tatccgccaa gcatgatgct tccatcgctt gcaatagaca
     8581 gatgctcgct tcgcgcatag tccacgccag gaagggtctc agtgaagatt ccattcgacg
     8641 caaagctcgt gtcgaggtcg ccgtctgctt ctactgcata gactgccaca tcgtctccca
     8701 cgtacccgcc aagcaggaag ccgccgtcct cgcgcatgac catgtcggta aggttcgtat
     8761 tcgcaaacgt agtcatgccg tttatgccga acgaagggtc gagaacgccc gcttgggtat
     8821 agcgcgcaag gaacgagtct ccctgatcgt tgtcctctcc aagcacaatg atgcgattgc
     8881 tgctgtcgac ttcgaccgca aggaagctgt actggtctcc tatcagaagc gcgtacccgt
     8941 catgggtcgt gccgctttcg ctgtaatggt tgccgaacga aggatcgaga acgcccgcag
     9001 aggatagccg cccgatgaag ccgccgtagc tatcacttgt gttgccgtaa tttcccacga
     9061 agaggatctg actcccctgg agcgctatgt ctcgcaccac agcaaggtcg ttgatgtcga
     9121 atgtgaacga gcctccgttg ccaaacgaag tatcaagcgc tccagttgcg gtaacgcgca
     9181 cgatgctcga tggataggga tccgggccca caaaagaatt cgcggagaag atgattttcc
     9241 catcaggctg cttcaggagc cccgttacct caggctcgct atcaggaagt cctacctgag
     9301 ccgcagtgaa gaacacagag ccatcagtat caaatgccgt atcaaggcca ccatacgcag
     9361 cgtgaacaaa tgtagatggg attgcgaaaa gcgatagaag catcgcagca acaaatgcgg
     9421 tatttcggtg cattactacg tatctagaga gcaatggttt tagtatacaa ggccagatag
     9481 cagaaagacc gcttgtgggc ggtctttctg cattacgcgt gttgcggggg ccggaatcga
     9541 accggcgtct ggaggttatg agcctcccga ggtacctctc ctctaccccg ctataggcat
     9601 ataatacgcc atatcctggc gtattgcaag tcctagatct ccccgtgctc gcgcatcacg
     9661 gtctcataga gcgcgattgt acggtgcgcc atatgttcct caagctgata tgcagaacca
     9721 gaatgtgcga tctcattacg taccttatgc gcgtcccacg cgttctgaag cgtgtggaaa
     9781 cgagacggat ccacggattt gagcttctcc cctactgaat cgcccggata cccaagctgt
     9841 gagagaagat cgtcgagcat aatatcggct tcgatgattg cttggcgcca atcactctca
     9901 tgtgggctct cgataagctc tcgcacatgc gcccagcgac tatgatcacg cttctcctca
     9961 gcatgctccg catgaagcgt cgcgtggcgc acttcgtcct catgctcaat ctgatggtac
    10021 ctgatgaccg catttacaaa cacgatgagg agcgcaatag agataactaa cgagagcgct
    10081 gttacgagaa gccagatctg acttgcgagt gccataaagc cacctgcttc aaacacaatt
    10141 ccgcgcggac tcgtgatgag ctcgtagaag agcctgaaga agtactcgac gttgagaaag
    10201 tgtacggtcg gagcggtagt cgttcccatt gagacgatta tagcgtctct ccggtcacat
    10261 cggcgccgat tctaaacagt acgtcgtccg caccgagtgg agcggtgaaa tggataccgg
    10321 tcgggagtga tactgtatcg cgcgtaacat ctcccattgg taccgaaagt gccggcattc
    10381 ccgtgaggtc cgcagtaact gtgaacacgt cttcgagata catagacacc gggtcgctct
    10441 tctctccgaa acggaatgct ggagacggtg tcgtcggaaa tgcgatcaca tcgcaagact
    10501 taaacgcctc ttcgtactcg gcgcgaagca cgtcacgtgt acgctcagcc ttgcggtaat
    10561 acgcatcgat gtatccagag gagagcacaa atgtacctaa aaggatgcgg cgtaccgttt
    10621 ccctgccgaa ccctgcggtc ttggatttca catagtcctc aagcaatgtc tctccgcgct
    10681 cggcaagtcc gtagcggata ccgtcaaagc gtgcgaggtt tgtcgatgct tcagcaggca
    10741 taaccacgta gtacgcaggg agcgcatagc cactcgtagg gagtgatacc tctacgagcg
    10801 tatgaccctt tgctttgagc gcatcaagtg tcttcctgaa attcgctgcc acgtcctcgt
    10861 ccaaaccttc cttgagtaga tgctccggaa caccgatgcg aagcgctgta tgctctgttg
    10921 gcttcgggta ggtatgctcg tcgattgtcg tggcatcgtt cgcatccttt ccacgcatcg
    10981 catcaaaaag aacctttgcg tctgcaactg tttttgcgat aggacccgcc tgatcaagcg
    11041 agcttccaag cgcgatgaga ccgaagcgag aaatcgctcc gtatgtgggc ttgagtccta
    11101 ttagtccggt atgcgccgct ggctggcgta tcgagccacc tgtatcagta ccgagtccgc
    11161 caagcgccat atcggcacag atcgcagatg cagagcctcc ggaagaacct cctgcggtgc
    11221 gagtcgtatc gataggattt cgagcgacgc cataggccga gttctcggtc gaagaaccca
    11281 tagcaaactc atccatattc gtgcgtccga taaagagcgc tcccgcgtcc ttaagtttct
    11341 tcgtgattgt cgcgtcgtac accgcgcgat ggttctcaag cattcgtgaa gctgcgctcg
    11401 cgattcttcc ttcaatgagg atgttgtctt tcattgcaag cggaatgccg cagagcattg
    11461 gtgcacgctc tccttcctca tcaatacgct tctgtgcagc tgctattacg tcctcatcag
    11521 catcaaatag ctcgaggtat gcattcaatt caggattctt ctccctcgca gcggtagcgc
    11581 acgcatccca gagttcgcgc acagaaaact ccttcgcgcg aagccccttc gccgcctcgg
    11641 ttatggaaag ctcgttcaaa ggcttagtca tggctgatga tctgcttcac cttgagcgaa
    11701 tccccttctc gatccgggaa ctgcgcggtt atagcgtcag tccaggtgcc gggagcgctt
    11761 gggttctcgt ctgcgcgtag tacgttgcgc accgaagaaa ggtttctccc ggtctcaggc
    11821 aggtcgagct cctcgagctt tcctacatac tcgaggatgc tatcgaactc ggccgcaaag
    11881 cgcggaagat ccgcttccgg aacctcgaca cgggcgagcg ctgccaggcg cttcacgtct
    11941 tctgcggatg ctttgctcat atataggtta ggtatatcac gaaccgaccc tccggaggaa
    12001 ttccgcctca tcgatcacgg atacgccaag ctcctccgct ttcgcaagct tgcttcctgg
    12061 cttctcgcct gcgaccacga atccggtctt cttcgatacg gaagacgcgg tctttccacc
    12121 cgctctgcgc accattgcct cgccctcttc gcgagagaac gactcgaagg acccggttat
    12181 gacaactgac ataccctcga gcggacctcc ggtgccttcc ctctctattg cagagaccga
    12241 gagatgcgcc gaaagtcggt cgaggagcga ttggttctct ggatcggcga accaatccga
    12301 gagcgcttcc gctaccacct ctcccacgcc atcgatgtcc gagaacgcag aagctttcgc
    12361 tgcacggagc gcatcgagcg tgctgaactc gatagcaatc acgcgagcgg tctcctcacc
    12421 cacgtgcggg atagagagtc ctacgagaag ccggtcgagc gggatctgct tcttatccgc
    12481 aagcgcgtca ataagattct gtgctgactt ctctttgaag ccgggcaact gtagcaactc
    12541 gtccttggtg aggtcgaaga aatcgtccat gtttgagacg agctcatgct ccatgagaag
    12601 ctttacggtc ttctccccga gcccgtcgat atcaagcgcg ctcttcccgg caaagtgcga
    12661 gagcttccgg agctgttggt caaaggatcc agcaaccgcg cagcgccgtg ccgcagtgcc
    12721 cggaacacgt tcgatcgtac cgtctccccc acacaatggg gacgaggctg gaaacttcca
    12781 cttcttctcc ttgcccgtcc ggaactccgg caacacctgc acgatctcag ggatgatgtc
    12841 tccggccttc tgaaggatca cggtatcgcc cacgcgtata tccttgtccc ggatgaaatc
    12901 ctcgttatgc aacgttgcac gcgcaacaat agtacccgca actgcgaccg gcttgaggtg
    12961 cgcgactggg gtgagtacgc ccgtacggcc tacttggagg ctgatgtcct cgataatggt
    13021 tgttacctgc tctgctggaa actttaaagc gatcgcatac cgtggccctt ttccagtgtg
    13081 tccgagggct tcctggtgag cacgactatc gaccttcact actacaccat cgagttgata
    13141 gtcctctttc tctcgagcac tgccttgcca cttcttccag aacgcgagta tctcctcgac
    13201 tgacgaagcg tgcttctcat gcggattaac cgggagtccg agtgaacgca ggtacgaaag
    13261 ctcctctgct tgcgttctcg gtatctcttc agaaagacct tcgatgtcgt agagaaatgc
    13321 cccaagcgga cgcgctgcag ctactgacgc atcgagctgg cgcaaagagc cggccacgat
    13381 atttctcgga tttgcaaaga gcggctttcc agctttctct tgagcacgat taagtgcttc
    13441 aaatcctttc ctgctcatat aaacctctcc ttcgacaatg agatcgactg gacgcaggag
    13501 gtgcttcgga atgctcttga tggttgaagc tgtgtgcgtc acgtcttcac cgacaacacc
    13561 gtctcctctg gtcgctgcaa gcacgagctc gcccttctca tatgtgagga tgatgtgcgc
    13621 tccatcgatc tttagttcgc aggtatacgc aggttctgtc gaaccgccaa aagacttcgc
    13681 aagacctttc cggacccgct catcaaacgc tctgagttca gcttcatcga acgcatcgtc
    13741 cagagaccac tgcggtacct ggtgatggac cttttttaga cccggaagcg ctttgccggc
    13801 aacagtt
//
LOCUS       DASWXU010000053        12612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_1008260, whole genome shotgun sequence.
ACCESSION   DASWXU010000053 DASWXU010000000
VERSION     DASWXU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008260"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VGE48_01850"
     CDS             complement(<1..915)
                     /locus_tag="VGE48_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY1042144.1"
                     /translation="MRGTVVGVLRGGPSREHEVSLKTGHAIIQNLPEDRFTVRDLYID
                     RNGVWHERGRVTDPVRVLPQIDVAVIGLHGEYGEDGEVQKLLERHGVPYTGSDSFASY
                     LAMHKVLTKEKAKELGLLTPRYRFVEQGGDTHAAAMEIVRSFHQPVVIKPVRWGSSVG
                     ISIVGGFQPVYDALEALLKEDAGGVLVEEFIRGTEATAGVVENLRGEPLYALPPIEIV
                     PPQGDFFSYGAKYSGATREIVPGRFAKPVQEELMRQAQVMHEGLGLRHYSRSDFMVTP
                     KGIYFLETNTLPGMTTESLLPKSLGAIGV"
     gene            complement(1288..2550)
                     /locus_tag="VGE48_01855"
     CDS             complement(1288..2550)
                     /locus_tag="VGE48_01855"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042145.1"
                     /translation="MQREDLKKFDLPDCPGVYLFTYGKGKAKKVLYVGKATSLRDRVR
                     SYFDIELIATRGPRIVDMVTKADGISYEATPTVLEALVREAALIREHMPPANAMGKDD
                     KTFLYAVITDEEIPRVLAVRGAEIDMKARRNILTGEAYKAVHGPFPSGYQLREGLKLI
                     RRIFPFYDTQKPVGEGNKHLLAKVEFNRQIGQYPKDMSRSEYLRSIRNVSLYLSGRVK
                     ALRAQLERDMKAFAKEERFEEAAQARKQLFALDHIQDVSLIKEDRGGENVGPRIEAYD
                     TAHISGTNAIGVMTVVENGMPQKKDYRTFRIRGYGGKSTNDDIASLKEILSRRLGHPE
                     WPLPKAFIVDGGKTHKKAAEEVLKEAGIGLPVIAVVKDEKHRPREVLGGMRAGVSDAD
                     AVLANSEAHRFSITKHRQARSRDLRKSS"
     gene            2640..2834
                     /locus_tag="VGE48_01860"
     CDS             2640..2834
                     /locus_tag="VGE48_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042146.1"
                     /translation="MLRRPLDPVFDVIRYHMKADEPSACLDIRIIGRSLKAFARRKQE
                     YCLVIDQQIFTRELILQDAS"
     gene            3007..3222
                     /locus_tag="VGE48_01865"
     CDS             3007..3222
                     /locus_tag="VGE48_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042147.1"
                     /translation="MQTQSTSDLKSVLDSLSERWSHLNGITLRLHGPFWVEVFTNFYG
                     QPPSGDTDRKWDKLFSMGFPLLREREA"
     gene            complement(3256..5916)
                     /gene="uvrA"
                     /locus_tag="VGE48_01870"
     CDS             complement(3256..5916)
                     /gene="uvrA"
                     /locus_tag="VGE48_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY1042148.1"
                     /translation="MAKAKKPHEQHTGEDWIRVKGARTHNLKNVSTEFPRGAMTVVTG
                     LSGSGKSSFAFDTIFAEGQRRYVESLSAYARQFLNQMQKPDVDEITGLAPAISIDQKS
                     RSNNPRSTVATVTEIYDYLRVLYARAGQPYCVNHDVPIEKLSKEEMIRLIQDRVNKRA
                     KGKKDEVMGVAVDKSAVSVYAPVVVGRKGEYYQLLYDLLGKGFERVLIDGKPHSLREK
                     IELDRNKRHDIDAVVDAIYVSEFERNPEAARERLSEALELALKESDGLVKIGHADGSE
                     ETLSAKFICPVDGASFPEVEPRLFSFNSPYGACPTCNGLGTKSLFSDDPCPTCEGKRL
                     RPEALRVYLSTKKKMPVDPDNITDVGTNVVDFVGMSIKEAKDFMDTLELSEMKKEIAL
                     PIVREITSRIDFMLNVGLDYLTLNRSAATLSGGEAQRIRLASQLGTGLVGALYVLDEP
                     TIGLHQRDNDRLIKTLVDLKELGNTILVVEHDEDTIYASDYILDIGPGAGVHGGEVIV
                     SGWLDDLLTAPKNDSGSNTLSYLREETRIELPEERRTGEKGSIKIKGATLHNLKNQNI
                     EVPLGKLVCITGVSGSGKSTFLYDVLHKNVAARLSKRQAEVENAASITGTEYIGRAVL
                     IDQSAIGRTPRSNPATYTGAFTHIRDLFAATPEARARGWKPGRFSFNVAGGRCEACQG
                     NGMIAVEMHFLPTVYVTCDVCNGTRYMKETLEATYRGKSIYDVLQMTIEEAEEFFKDI
                     PAVADRLQSLNATGLQYLTLGQSATTLSGGEAQRVKIASELYRPITMKTLYLLDEPTV
                     GLHYEDVRKLIEILQELVRRGNTVVVIEHNLDVIKSADYLIDFGPEGGTGGGKVIAKG
                     TPEEVAEAEGSHTGEYLAKLLKKHKKPLKK"
     gene            complement(5996..6379)
                     /locus_tag="VGE48_01875"
     CDS             complement(5996..6379)
                     /locus_tag="VGE48_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1042149.1"
                     /translation="MADTKFILVVEDDPILKNLLGHTLAGKYQTLYASDGNEAIALLE
                     ANKPVLVLLDLMLPTMDGFAVLQSIRARTDELKSVPIVVVSNLGAEADIEKAKSLGAN
                     DYLIKAEVAVEEIVAKIESMLTPSA"
     gene            complement(6386..7468)
                     /locus_tag="VGE48_01880"
     CDS             complement(6386..7468)
                     /locus_tag="VGE48_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1042150.1"
                     /translation="MTIRVFTALLLVCVLTLAGLAIFVSGLFLRDQLAPLVTDIEANQ
                     ELMLVILEISVASVVLVLAFISLLFFAVGHFIAKPIRLVANAMRAFAETGQVPAPMTA
                     WAPKEVRELEVAFSGYTGRVDEVHKHDVEISRVKSDFISTAAHQLRTPLTGIRWALEA
                     LQMEQLTESQKALVASAVEKSHQLVSVVGTLLDISSIESGKYKYDMKRTRINDILEEV
                     ARDFAPQAAAAKVSFVFEKSGPESAVVSVDRERIRWVLNNLVENAIRYTPAGGSVRIT
                     SDAAAGRAYVRVHDTGIGIPDTDRANIFTRFYRASNAVSKQNGGNGLGLYIARTIATD
                     HGGDLNFEANANGPGTTFTLSLPVLE"
     gene            complement(7486..9546)
                     /gene="uvrB"
                     /locus_tag="VGE48_01885"
     CDS             complement(7486..9546)
                     /gene="uvrB"
                     /locus_tag="VGE48_01885"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY1042151.1"
                     /translation="MATFKIHSPFPPGGDQPKAIEKLTKGIEKGLRHQTLLGVTGSGK
                     TYTIANVIQNVGKPTLILAHNKTLAAQLAQEYREFFPENAVHYFVSYYDYYQPEAYIA
                     HSDTYIEKEAQINAEIDRLRHAATQALLTRKDVIIVASVSAIYGLGSPAEYEKRHVKL
                     QVGAPEDRAALIRKLVGIYFERTNADLEPGKIRAVGNAVELMPTNERAIYRITFDGPV
                     VGRIECLDPVSRSLTGTPDSLFVFPAKHFVTDEEERARAIEDIRRELDERLDTLKREE
                     KVLEHERLKRRTLHDLAMIRELGYTNGIENYSRHFDGRAPGEPPHTLLSYFPHKKDGS
                     PDFLTVIDESHVTVSQIGGMYAGDRSRKDVLIEHGFRLPSARDNRPLKFEEFEERIGQ
                     VIYTSATPGTYEKEHSKEPEGQVAEQIIRPTGLVDPELVVKPIVEGDGYRGQVADFID
                     EAEVVIKRGGRALATTLTKQMAEDLAEFLRERGMKAEYLHSDVKTIDRIEILTAFRKG
                     DFDVLVGVNLLREGLDLPEVELVGILDADKEGFLRSETSLIQTIGRAARNILGKVVLY
                     ADTMTGSMERAIGETERRRAIQVAYNKEHGIMPATVKKEIRDIAESMRTEHQKTVANL
                     LDLDQKLYKEDPVAFIKDKRQQMADAVEALDFETAAILRDEIYALEAGEAPKKKAPLR
                     RRGR"
     gene            complement(9589..10803)
                     /gene="tgt"
                     /locus_tag="VGE48_01890"
     CDS             complement(9589..10803)
                     /gene="tgt"
                     /locus_tag="VGE48_01890"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY1042152.1"
                     /translation="MRPISFAIDAKEGNARAGVLTTPHGSIKTPAFVPVGTKAGVKGV
                     LPSQLKELGAQVVLANTYHLYLQPGEKTVKDAGGLGSFMAYDGPTMTDSGGFQVFSLG
                     AAFGRTITKIAKGEENARDQGVTVYDEEVQSQHGQLAVIDEEGVTFTSHHDGSLHRFT
                     PERSIEIQHALGADMFFAFDECTSPTESYEYQRDAMRRTHKWADRSLKAHRQNIDANR
                     KQGIFGIVQGGRYEDLREESAKEIASMDFDGFGIGGSFSKDDMMGALQAAVQHLPEEK
                     PRHFLGIGEPGDLLLGIQEGMDTFDCVAATRMGRHGSIYTKRGVINLKNEKFKNEYEP
                     LDAETLVPGTEGFSRAYVAHLIRSGEMLGQIIASMHNLGFIIQLVDGAREAIQENRFS
                     AYKEDFERLYYA"
     gene            10948..11559
                     /locus_tag="VGE48_01895"
     CDS             10948..11559
                     /locus_tag="VGE48_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042153.1"
                     /translation="MITFKTATRGLALAALFAGLAACGTPGAVATNGSAGSGTFMVVR
                     YEGLSSVQGSRPARWTQTEIDQVMELDQVCRNQLDPQFPNKVEAIARGAAENSLWSFL
                     GIGGGSGALPGADPADYGPYGAAAGAASGAFAGSMSYDNARKYAQSYCVSLQVGWAQN
                     EGALHGAGIVPWVSYGRPRIPQAVGDPSAEGAVAGQTNRPPPR"
     gene            complement(11635..12150)
                     /locus_tag="VGE48_01900"
     CDS             complement(11635..12150)
                     /locus_tag="VGE48_01900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011360300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY1042154.1"
                     /translation="MNPTQTGIAVALAVVVALGFLVFGTPILGSFGGMNLAPQETVGT
                     TTNLSELPMDLSLAPGEPLPTDLVITDVVVGSGPEATPGQMVTVNYVGALPDGTIFDA
                     SANHGQPFAFQLGAGQVIRGWDEGVAGMKVGGKRRLVIPPDMAYGNQAVGGVIPANAT
                     LLFEVELLAVQ"
     gene            complement(12153..12458)
                     /locus_tag="VGE48_01905"
     CDS             complement(12153..12458)
                     /locus_tag="VGE48_01905"
                     /inference="COORDINATES: protein motif:HMM:NF039821.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HEY1042155.1"
                     /translation="MNTDYRTILQTLDVGALPEEDQQSLLEELHELVFKGSLVRMIEQ
                     MDDATREEFEALMASDADEETVDTFLKERVPGADKAVEDTMRELAGDILSGTGTNQE"
BASE COUNT         2654 a         3716 c         3370 g         2872 t
ORIGIN      
        1 caccccaata gctcccaagg acttcggaag gagggattca gtggtcatac ccggaagggt
       61 gttcgtttca aggaaataga tgccctttgg ggtaaccatg aagtcagaac gtgagtagtg
      121 acggagtccc agtccttcat gcatgacctg cgcctggcgc atcagctcct cctgtacggg
      181 cttggcgaac ctacccggca caatctcacg cgtagccccg gagtacttag ccccgtacga
      241 gaagaagtct ccttgcggcg gaacgatctc aataggcgga agtgcataca gtggctcccc
      301 gcgaagattc tcaactacac ctgcggttgc ttctgttcct cggataaatt cttcgacgag
      361 tacgccgccc gcgtcttcct taagaagcgc ctcaagggcg tcatataccg gttggaaacc
      421 acccacgata gaaataccta cggacgaacc ccatcgcacc ggctttataa caactggctg
      481 atggaatgaa cgcacgatct ccattgctgc agcgtgcgtg tcgcccccct gctctacgaa
      541 ccgatatcgc ggcgtcagaa gaccaagctc ctttgccttc tcctttgtaa gcaccttgtg
      601 cattgcgaga taggacgcaa atgaatcgga gccagtgtat ggaaccccgt gacgttcaag
      661 gagcttctgg acttccccgt cctctccgta ttcgccatgc agaccgatga cggcaacgtc
      721 gatctgtggc agtactcgga caggatctgt gacacggccg cgctcatgcc acacaccatt
      781 gcgatcaatg tagaggtcac ggaccgtaaa gcggtcttcc ggaaggttct gaatgatggc
      841 atgccccgtc ttgagagaca cctcatgctc cctggaagga ccgcctctaa gaacgccaac
      901 aaccgtgccg cgcatatgcg gaaagtatag caaaggaaaa ggccgtcgct tggagctaaa
      961 cggccttcgc cctcgtggga ctgaatctct ctaaatccct atgggctttt cagggacttg
     1021 cgtccactgt gtagatgata gcgatgtggg taggcactcc atgagtggtg gtgaaggttc
     1081 ccatctccga ggccttatgc gctcaggaat cttcagcacc tccagggcag ttcgccgcta
     1141 tcgcgggtcg ctgctcctga agctccaact tcgcacgcac acgcggaaca acattctttt
     1201 cctcccctgc tgtctcgagc acgccaagcg tgcccggtga ccctcaagac tggtcaccag
     1261 gcgagaatag cactctgtag aaatttgtca cgagcttttc ctcaggtccc tggatcgggc
     1321 ctgccgatgc ttcgttatgg agaaccggtg cgcctcagaa ttcgcgagga ctgcgtcagc
     1381 atcagagacg cctgcacgca tgccgcccag aacctcgcgg ggtctgtgct tctcgtcctt
     1441 gactaccgcg ataaccggaa gaccgatacc cgcctccttg agcacttcct ctgcagcctt
     1501 cttgtgcgtc tttccgccgt ccacgatgaa cgctttcggg agcggccact cgggatgccc
     1561 gagcctgcga gaaagaatct ccttcaagga cgcgatgtcg tcgttcgtgg acttgccgcc
     1621 gtacccacgg atcctgaacg tgcgatagtc cttcttctgc ggcatgccgt tctcgacaac
     1681 cgtcatcacg ccaatcgcat tcgtacctga gatatgggca gtgtcatagg cttcgatgcg
     1741 tgggcccacg ttctctcctc ctctatcctc cttgatgagc gagacgtcct ggatatgatc
     1801 gagcgcgaac agctgcttgc gcgcctgagc cgcttcctcg aagcgctctt ccttggcgaa
     1861 ggccttcata tcgcgctcaa gctgcgcacg gagcgccttc acacgccctg agagatacaa
     1921 cgacacgttt ctaatggaac ggagatactc ggatcggctc atgtccttgg ggtactgacc
     1981 gatctgtcga ttgaactcaa ccttcgccaa gaggtgcttg tttccctcgc caaccggctt
     2041 ctgggtgtca tagaacggga atatacgtcg aatgagttta agaccctctc taagctgata
     2101 cccggacggg aatgggccgt gcactgcctt gtacgcctct cctgtgagaa tgttgcgcct
     2161 tgccttcatg tcgatctcgg caccccgtac cgcgagcaca cgcgggatct cctcgtctgt
     2221 tattacggca tacaggaacg tcttatcatc cttgcccatg gcattcgcag gaggcatgtg
     2281 ctcccggatg agtgccgcct cacgcaccaa agcctcaagc accgtgggtg ttgcttcgta
     2341 ggaaatcccg tctgctttcg taaccatatc cacgatgcgg ggaccgcggg ttgcaatgag
     2401 ctcgatatcg aaataggagc gtacgcggtc tctcaaagac gttgccttac ctacgtatag
     2461 gacctttttt gcctttcctt tcccatacgt gaagagatac acgcccgggc agtccggtag
     2521 gtcgaatttc ttgaggtctt cacgttgcat gtacgtatcc taccctgcag accctaaagg
     2581 gaaagacctg cgcatggcag gtctcccgac ccttaggccg caaggagcga gcggatgaga
     2641 tgctcaggcg cccgctcgat ccagtatttg atgtgatccg gtatcacatg aaggcagacg
     2701 aaccgtcggc atgcctcgat atccgtatca ttggtcgctc tcttaaagcg tttgcgcgca
     2761 gaaagcagga atactgcctc gtcatcgatc agcagatctt cacgagggaa cttatccttc
     2821 aggacgcgtc gtagcgtaga tttgaggtca cggaacttca ccgcgtcccg agagacagca
     2881 gcccagtcga ttcgatgcag tgtaacggtc gatgccatgt gatccctcca agaacatggt
     2941 gcctgaaacc tagcctagca cgagtgctat acttaggctt tggaatagct ccacgggagg
     3001 gttgcgatgc aaactcagtc aacttccgac ctcaagtcag tactcgattc tctgtccgaa
     3061 cgatggagtc acctgaacgg catcacgctt cggctgcacg gaccgttttg ggtagaagtc
     3121 ttcaccaact tctacggcca gcctccttcg ggggatacgg accgcaaatg ggacaaactc
     3181 ttcagcatgg gattcccgct gctgcgcgag cgagaggcat gacagagaag gggacgcgca
     3241 ctgcgtcccc tttccctatt tcttgagtgg tttcttgtgc ttctttaaaa gcttcgcaag
     3301 gtactcgccg gtatgcgagc cctcagcctc agcaacctct tctggcgtac ccttggcgat
     3361 aaccttaccg cctccggtac ccccttcagg accaaagtcg atgagatagt ctgcggactt
     3421 gatgacgtcg aggttatgct caatcaccac aaccgtgttc ccacggcgca cgagctcctg
     3481 gaggatctcg atgagcttgc gaacatcctc ataatgaaga cccactgtag gctcgtcgag
     3541 caagtacagc gtcttcatcg tgatggggcg gtacagctcg ctagctatct tcacgcgctg
     3601 cgcctcgccg cctgaaagcg ttgttgcgga ctgcccgaga gtcaggtact ggagaccggt
     3661 cgcgttaagg ctctgaagcc ggtcagcaac tgcaggaatg tccttgaaaa actcttccgc
     3721 ttcctcgatc gtcatctgca gcacgtcata gatgctcttc ccgcgatacg tcgcctcgag
     3781 tgtttccttc atgtagcgag tgccgttaca gacgtcacag gtcacataca ccgtcggaag
     3841 gaaatgcatc tccaccgcga tcatgccatt gccctggcac gcttcgcagc gtccgccagc
     3901 aacattgaac gagaagcgtc ctggcttcca gccacgcgca cgggcttcag gagttgctgc
     3961 gaataggtcg cggatatgcg tgaacgcacc cgtgtaggtc gccggattcg agcgcggggt
     4021 gcgcccaatc gcagactggt cgataagcac agctcggccg atatattcgg tgcctgtgat
     4081 gcttgctgcg ttctctactt cggcctggcg cttggagaga cgtgctgcca cgttcttatg
     4141 caatacgtca tagaggaatg tggacttgcc cgagccggac acgccggtga tacaaacgag
     4201 cttcccaagt ggcacctcga tattctggtt ctttaggttg tgtaaggtag cgcccttgat
     4261 cttgatcgag cctttctcgc cggtacgacg ctcttcaggc agctcaatac gcgtttcctc
     4321 gcgcaggtac gagagggtat tcgagcccga atcgttcttc ggtgctgtca gaagatcatc
     4381 aagccaccca gagacaataa cctcaccccc atgcacgccc gcacccggac cgatatcaag
     4441 gatgtagtcg gacgcgtaga tcgtatcctc atcatgctcc accacgagga tcgtgtttcc
     4501 aagctccttg aggtccacaa gggttttgat aagacgatcg ttatcacgct ggtgcagacc
     4561 aatggtcggc tcgtcgagta catagagtgc gcccacaagt ccggttccga gctgggacgc
     4621 gaggcggata cgctgcgcct caccaccaga aagggtcgct gccgagcggt taagcgtgag
     4681 gtagtcgaga ccaacgttca gcatgaaatc gatacgactg gtgatctcgc ggacgatcgg
     4741 aagcgcaatc tccttcttca tctcagagag ctcgagcgta tccatgaaat ccttggcctc
     4801 cttgatagac atgcccacga aatcaaccac gttcgttcct acatccgtga tgttgtccgg
     4861 atcgacaggc atcttcttct tggtcgagag gtacacacgg agcgcttccg ggcgcagacg
     4921 cttaccttca cacgttggac acggatcatc cgagaacaac gactttgtac caaggccgtt
     4981 acatgtagga caggcgccgt acggactatt gaaggagaag aggcgtggct caacttcagg
     5041 gaacgacgcg ccatctaccg gacagatgaa ctttgcagaa agcgtctctt cggaaccgtc
     5101 tgcgtgcccg atctttacga gcccgtcact ttctttgagt gcaagctcaa gcgcctcaga
     5161 aaggcgttca cgtgccgctt caggattgcg ctcgaactca gacacataga ttgcatccac
     5221 cacggcgtcg atgtcatggc gcttgttgcg atcgagctcg atcttctcac gaagcgagtg
     5281 tggctttccg tctatgagca cacgctcgaa accctttccg agaaggtcat acagaagttg
     5341 gtaatactcg cctttacgac caaccaccac cggtgcatag accgatacgg ctgatttatc
     5401 cactgccaca cccatgacct cgtccttctt acccttcgcg cgcttattta cacgatcttg
     5461 gatgaggcga atcatctcct ccttcgacag cttttctatg ggtacgtcat gattcacgca
     5521 gtatggctgc cctgcacgcg catagaggac gcggagataa tcgtagatct ccgtcacggt
     5581 cgcaaccgtc gagcgcgggt tattggaacg agacttctgg tctatggaaa tggcaggagc
     5641 gagtcctgta atctcgtcca cgtccggctt ctgcatctga ttaaggaact ggcgcgcgta
     5701 cgctgaaagc gactctacgt atcggcgctg tccctcggcg aagatggtat cgaacgcgaa
     5761 cgatgacttg ccggatcctg agagtcccgt gaccacggtc atagctcccc gcggaaactc
     5821 cgtggagacg ttctttaagt tatgggtgcg cgcacctttc acgcggatcc agtcctcgcc
     5881 cgtatgttgt tcgtgtggtt tcttcgcttt tgccataagt atggaatgag gataccccgt
     5941 ccttggcggg acgggtacac aactattata ccatttatgt taaggaagcg tgaggctacg
     6001 cagaaggcgt aagcatgctc tcgatcttgg ccacaatttc ttcaaccgca acctctgcct
     6061 tgattaagta atcgttcgcg ccgagtgact tcgctttctc gatatccgcc tccgcgccga
     6121 gattcgatac cacgacgatc ggcacgctct tgagctcgtc cgtacgcgct cggatgcttt
     6181 gcagcactgc aaacccgtcc atggtcggga gcatgagatc aaggagcacc aataccggct
     6241 tattcgcttc aaggagcgcg atagcttcgt tcccgtctga cgcatacagc gtctgatact
     6301 tgcctgcaag cgtatgcccg aggaggttct tgaggatcgg atcgtcctcc actacgagaa
     6361 tgaatttggt gtctgccata ccctcctatt ctagcaccgg caaggacaac gtgaacgtgg
     6421 tgcccgggcc gttggcgttt gcctcaaaat taaggtctcc gccgtggtcc gtagcgatag
     6481 tacgggcgat gtacagccca agcccattgc cgccattctg ctttgagacc gcgttagacg
     6541 ctcggtagaa acgcgtaaag atatttgccc tatccgtgtc cggaatgccg atgcccgtgt
     6601 catgcacccg gacatatgca cgtcccgctg cagcgtcgct cgtgatgcgc accgagcctc
     6661 cggctggcgt atagcggata gcgttctcta cgagattatt cagaacccag cggatgcgct
     6721 cacgatctac agaaacgacc gcgctttcag gccctgactt ttcaaacaca aaagacacct
     6781 tcgctgcagc agcctggggt gcaaagtctc gtgctacttc ttcaaggatg tcattgatcc
     6841 gggtacgctt catgtcgtac ttgtacttgc ctgactcaat actcgagata tcaaggagcg
     6901 tccccactac ggacaccagc tgatggctct tttcaaccgc acttgcaacg agcgccttct
     6961 gactttcggt aagctgttcc atctggagtg cttcaagcgc ccaccgtata ccggtaagcg
     7021 gcgtgcgcag ctggtgagcc gcggttgaga tgaagtccga cttcacgcgt gatatttcca
     7081 catcatgctt atgcacttca tccactcgcc cggtatagcc actgaacgcc acctcaagct
     7141 cgcgcacctc cttaggtgcc catgcggtca tgggagcagg cacctgccca gtctccgcaa
     7201 acgcgcgcat tgcgttcgct acgaggcgga tcggcttggc gataaagtgt cccaccgcaa
     7261 aaaacagcag actgataaag gcgagtacga ggacgacaga agccaccgag atttcaagga
     7321 taacgagcat cagctcctgg ttcgcctcga tatccgtaac cagaggtgcg agttggtccc
     7381 taaggaaaag gcctgagaca aaaatagcga gtcccgcgag cgtaagcacg cacacaagaa
     7441 ggagcgcggt aaaaacgcgt atggtcatgg cattatgcta gcacgctagc gcccccgtct
     7501 cctgagcggg gctttcttct tgggtgcttc tcctgcttca agcgcgtaaa tctcgtcgcg
     7561 gaggatagct gcagtttcaa agtcgagtgc ctcaaccgca tctgccattt gctggcgctt
     7621 gtctttaata aaggcaacag gatcttcctt gtaaagcttc tggtcgaggt cgagcaggtt
     7681 cgctaccgtc ttctgatgct ctgtgcgcat ggattcggcg atatcgcgga tctccttctt
     7741 caccgtagcc ggcatgatgc cgtgctcttt gttataggca acctgtatcg cacgacgtcg
     7801 ctccgtctcg ccaatagcac gttccatcga gccggtcatg gtatctgcat acagcaccac
     7861 ctttccaaga atattacgcg cagcacgtcc gattgtctga atgagcgacg tctcggagcg
     7921 taggaaccct tccttgtccg catcaaggat gccgacgagc tccacttccg gaaggtcgag
     7981 accttcgcga agcaggttca cgcccacgag cacgtcaaag tctcccttgc ggaacgcggt
     8041 taggatctcg atgcggtcga tggtcttcac gtcggagtga aggtattccg ccttcatgcc
     8101 acgttctcga aggaattctg cgaggtcctc tgccatctgc ttggtgagcg tggtcgcgag
     8161 tgcacgtcct ccgcgtttga tgactacctc tgcttcgtct atgaagtccg cgacctgacc
     8221 acgataccca tccccttcga cgatcggctt cacgacaagc tctggatcaa cgagccccgt
     8281 aggacgaatg atctgttccg ctacttgacc ttccggctcc ttagagtgtt ctttctcgta
     8341 cgttcctggc gttgctgacg tatagataac ctggccgata cgctcttcga actcctcaaa
     8401 ctttagaggg cggttgtcac gtgcgctcgg caagcgaaac ccgtgctcaa taagcacgtc
     8461 cttgcgagag cggtctcctg cgtacatgcc gccgatctgc gagacggtta cgtgagactc
     8521 gtcgataact gtaaggaaat cgggggaccc gtccttctta tgcgggaaat aggaaagtag
     8581 cgtgtgcggt ggctctcctg gcgctcgtcc atcgaaatgt cttgagtagt tctcaatgcc
     8641 atttgtgtag ccgagctcac gaatcatcgc aagatcatgc aacgtacgac gcttcaggcg
     8701 ctcgtgctcg agcaccttct cctcgcgctt aagcgtatcg agtcgctcat cgagctccct
     8761 cctgatgtcc tcgatagcgc gtgcgcgctc ttcctcgtca gtaacgaaat gcttggcagg
     8821 gaacacaaac agggaatctg gggtgcctgt aagtgagcgt gatacaggat caaggcactc
     8881 gatgcgtcct acgacaggtc catcaaatgt gatgcggtat atcgcgcgct cattggtcgg
     8941 catgagctcg accgcattcc ctacggctcg aatcttccca ggctcaagat ccgcattcgt
     9001 gcgctcaaaa tagataccaa caagtttcct aataagggca gctcggtcct ccggcgcacc
     9061 aacctggagc ttcacgtggc gcttctcgta ctcggcagga gacccaaggc cgtagatagc
     9121 agacacagat gcgacgataa tcacgtcctt gcgggtcagg agtgcctggg ttgccgcgtg
     9181 gcgcaggcgg tcgatctctg cgttgatctg tgcctccttt tcgatatagg tatccgagtg
     9241 ggcaatgtac gcctccggct ggtagtagtc gtagtaggag acgaagtaat ggacggcatt
     9301 ctcagggaag aattcccggt attcctgtgc aagctgcgcg gcgagggtct tgttgtgcgc
     9361 gaggatcagg gtcggcttcc ccacgttctg aataacgtta gcaatcgtat aggtctttcc
     9421 tgaacccgtg acgccaagca gggtctggtg cctcaagccc ttctcgatgc ctttggtgag
     9481 cttctcgata gcctttggct ggtcgcctcc cggcgggaat ggagagtgga tcttgaaggt
     9541 agccattgcg gtcagtgtac ctttcgcaaa tgaattaatg aagaagcgct atgcgtagta
     9601 caaccgttca aaatcctctt tatatgccga gaaccggttc tcctgtatgg cctcgcgtgc
     9661 accatctaca agctggatga tgaatccgag gttatgcatc gatgcgatga tctgccccag
     9721 catctctcct gaacgaatca ggtgtgccac atacgcacgt gagaaccctt cggttccagg
     9781 cacaagagtc tcggcatcaa gtggttcgta ctcattcttg aacttctcgt ttttgagatt
     9841 gatcacgccg cgcttcgtat agatgcttcc gtggcgcccc atgcgcgtag ccgcaacgca
     9901 atcaaacgta tccatgccct cctggattcc aaggagtaga tcccccggct ccccaatgcc
     9961 aaggaagtgt cttggcttct cttctggcag gtgttgtaca gcggcctgca atgcacccat
    10021 catgtcgtcc ttgctaaagg acccgccgat gccgaacccg tcgaagtcca tggacgcaat
    10081 ttccttcgcg ctctcctcgc gcagatcctc gtatcttccg ccctgcacga tgccgaagat
    10141 tccctgcttg cggttcgcgt cgatattctg acggtgcgct tttaaggaac gatcagccca
    10201 cttatgcgtg cggcgcatcg catccctctg gtattcgtag gattcggtag gcgacgtgca
    10261 ctcgtcaaag gcgaagaaca tatctgcacc aagcgcatgc tgtatttcta tagaacgttc
    10321 cggcgtgaag cgatgcaatg agccatcgtg atgcgaggtg aacgtgacac cctcctcgtc
    10381 gataaccgcg agctgcccat gctgtgattg cacctcttcg tcatacacgg tgacaccttg
    10441 gtcgcgtgcg ttctcctcgc ccttggcgat cttcgtgata gtacggccga atgcagcacc
    10501 gagtgaaaat acctggaatc ctcccgaatc agtcatcgtt gggccgtcat atgccataaa
    10561 cgaaccgagt cctccggcgt ctttaactgt tttctcgcct ggctgcaggt aaaggtgata
    10621 ggtattcgcg agtaccacct gcgccccgag ctccttcaac tgcgagggga gcacgccttt
    10681 aacgcctgcc ttggtcccaa caggaacaaa tgcaggtgtc tttatggagc cgtgcggcgt
    10741 ggtaagcaca cctgcgcgcg cgttcccttc tttcgcatct attgcgaacg aaatcggtct
    10801 cataccgcta accgtacggg ccaagagtca tttgagcaag gattttggac gtaccaaggt
    10861 acttgacaat gtattattta tatgtgtata gtatctagga tttgttcttt ccatcctgga
    10921 ttgagcacaa ctcagggaga taccccaatg atcacgttta agacggcgac gcgggggctc
    10981 gccctcgctg ccctgttcgc cggcctcgcg gcctgcggca ccccgggcgc ggtcgccacc
    11041 aacggctccg ccggttcggg caccttcatg gtcgtccggt acgagggctt gtcgagcgtg
    11101 cagggctccc ggcccgcgcg ctggacccag accgaaatcg accaggtgat ggaactcgac
    11161 caggtctgtc ggaaccagct ggatccgcag ttcccgaaca aggtcgaggc gatcgcccgc
    11221 ggcgcggcgg aaaacagcct gtggagcttc ctcggcatcg gcggaggctc cggagcgctg
    11281 ccgggcgccg acccggccga ctatggcccc tacggcgccg cggcaggcgc ggcttcgggc
    11341 gcgttcgcgg gctccatgag ctacgacaac gcgcgcaagt acgcccagag ctactgcgtg
    11401 tcgctgcagg tcggctgggc ccagaacgaa ggcgccctgc acggcgccgg catcgtcccc
    11461 tgggtctcct atggccgtcc gcggatcccg caggccgtcg gcgacccgag cgccgaaggc
    11521 gccgtggcgg gccagaccaa ccggccgccg ccgcgctgac tcaatcctga ctgcgtcaaa
    11581 aacgcacaaa gccccggaga gcgatctccg gggctttttg tatgcgcaca ttttttactg
    11641 caccgcgagc aactctacct caaagagaag tgttgcgttc gctgggatca cgccgcctac
    11701 agcctggttt ccatatgcca tatctggcgg aataaccaga cgacgcttgc cgcccacctt
    11761 catacctgcg acaccctcgt cccatccgcg gataacctgg cctgcgccaa gctggaacgc
    11821 aaacggctgg ccgtggtttg cagacgcatc gaagatcgta ccgtccggaa gggcaccgac
    11881 gtagttaacg gtgaccatct gaccaggggt agcttctggg cctgagccca caacgacgtc
    11941 agtgataaca agatcggttg ggagcggttc tcccggagcg agcgataggt ccataggaag
    12001 ttctgaaagg tttgtagtcg taccgacggt ctcctgagga gcaaggttca tgccgccgaa
    12061 cgagcccagg attggtgtcc cgaacacgag gaaaccgagc gccactacta cggcgagcgc
    12121 aacggcgatt cctgtctgcg ttggattcat agctattctt ggttagtgcc ggtacctgaa
    12181 agtatatcac cagcaagttc gcgcatagtg tcctcaaccg ccttatctgc tcctggcacc
    12241 cgctccttaa ggaacgtgtc taccgtctct tcgtcagcat cagacgccat gagtgcctca
    12301 aactcctccc gtgtagcgtc atccatctgc tcaatcatgc gcacgagtga tcctttaaac
    12361 acaagttcgt gcagctcttc tagaagagat tgctggtcct cttctggaag agctcccaca
    12421 tcaagcgtct gaagtatagt gcggtagtca gtattcatag ctgcagttcc tattgcacgc
    12481 gtctaatgta ctcctcagga cgaacctgca cctcaggatc tgagcttggc atgataccaa
    12541 tgccaccctg tccgtcagga gcgaatccaa ttgctctgcg caccgtgtta ccaagtgcat
    12601 ctatatacga gg
//
LOCUS       DASWXU010000054         9318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_502381, whole genome shotgun sequence.
ACCESSION   DASWXU010000054 DASWXU010000000
VERSION     DASWXU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502381"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..318)
                     /locus_tag="VGE48_01910"
     CDS             complement(22..318)
                     /locus_tag="VGE48_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042156.1"
                     /translation="MIAASAAALALAAMSSQAAATSPLDPLPDAGHASFVNQSQLTSG
                     ALIDDTGHIDRHRTDANIALTATDGLDSDMISDNVNDSIDLGGADGHRGHSAGT"
     gene            682..1323
                     /locus_tag="VGE48_01915"
     CDS             682..1323
                     /locus_tag="VGE48_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1042157.1"
                     /translation="MVITHHGGQCFKVTLGDRSLVFDPIAKGASLPAVRFGADIALVS
                     RKHKDMNGVAEVSFGGTEPFVIDGPGEYEVAGVTVQGFLTKSQYGLPKGETDAVNTVY
                     AVSLEGMTLVHLGALSDPMLSHDAREAIDDIDVLFVPVGGDGVLNAAEAARLAVTLEP
                     KVVIPMHWDGMGEPKALESFLKESGKESEKVDKLTLKKKDAADKDGAIIVITA"
     gene            1327..1704
                     /locus_tag="VGE48_01920"
     CDS             1327..1704
                     /locus_tag="VGE48_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042158.1"
                     /translation="MALDNLKGHIERLQEKPEHVRHQIAMGVAVGVTALVATGWAVAL
                     GTSGALALSNPEPVEGDGRDSLIESFEEPANAFTNLMGAAGAAFNATSTEAALKVIET
                     RTSSTLEGRAQPANNTEKTVIPF"
     gene            1710..4181
                     /gene="gyrA"
                     /locus_tag="VGE48_01925"
     CDS             1710..4181
                     /gene="gyrA"
                     /locus_tag="VGE48_01925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY1042159.1"
                     /translation="MPKKALKEEAPIEPRINVVDQSITSEMRSSYLDYAMSVITSRAL
                     PDVRDGLKPVHRRILYAMRQTGLTPSAKFRKSATVVGEVLGSYHPHGDTSVYDAMVKL
                     AQDFATRYLLVQGQGNFGSVDGDPPAAYRYTEAKMSRLADEMLKDLEKETVDWNPNFD
                     GSKQEPSVLPAAVPNLLLNGTLGIAVGMATNIPPHNLREVVDATTHLIDNPDASVDDL
                     LQHVKGPDFPLGAIAFNQADIKHAYTSGRGGVVVRGEAEIVEEKKGTNIVITSIPYRV
                     NKADLITRIADLVRDKKLEGVKDLRDESAADIRVVVELKGTAHPQAVLNNLYKYTELE
                     STFHYNMLALVDGVPQTLSLKGMLESFILHRQEVVKRRTEYELRKAEEREHILLGLSK
                     ALDHIDEVIKTIRASKDVPSASAALQKKFAFSQIQAAAILEMRLQKLAALERQKIEDE
                     LAEIQELIKRLKKILSSAKNILAVVREELVAIGEKYGDDRRTKIVKGGVKNISVEDLV
                     PDEESVLVLTAGGYVKRTNPDEYKVQKRGGVGVIDMSTKEEDVVTHFVKASAHAELLF
                     FTDFGKAYQLRMFDLPEGKRATKGKSIMNFLPLAGEERVTSVLAIPKGKERDGLSVFL
                     VTEAGTIKKVEAKSFADVRRSGIIAIALKGGDKLVSAHLAAAGDSVSIVTAEGQSIRF
                     DEAETRAMGRTAAGVRGMNVKKGDRVVSAEVVPGEAGDNAALLVIMDKGYGKKTSLSE
                     YKVQGRGGSGIKTADVTAKTGKIIGAKVLIGDAEKDDELVVISKKGQVIRCSAGEIPS
                     LSRATQGVRIMKMREGDNIASMVAL"
     gene            4201..4575
                     /locus_tag="VGE48_01930"
     CDS             4201..4575
                     /locus_tag="VGE48_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042160.1"
                     /translation="MGLEGRQQFGPDALRRVPDKTEGINPNTFEKRSLGERMALTGEE
                     SQAYQRMLMAGSQSELRAAIDAFNAISTPKVASELGFSLDDMQIIDEPVGIRPHDLPL
                     DAEVVATRDMREGVLRFRIKDR"
     gene            4680..5210
                     /locus_tag="VGE48_01935"
     CDS             4680..5210
                     /locus_tag="VGE48_01935"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1042161.1"
                     /translation="MNFSNPQSNVLQLGLREGMKVGDLGVGSGHYAASAAAIVGPEGR
                     VYAVDIQEDILKHVKDHAAGKGLRNVETIWGNFEKLGGTKLRDASLDAVILSNTLFQL
                     DHREGAVAEIKRILKTGGRLLVIDWAGAYGGMGPLPHRVVTEHVAEELFISAGFHKVK
                     DFRAGPHHYSIVFTAP"
     gene            5214..6515
                     /locus_tag="VGE48_01940"
     CDS             5214..6515
                     /locus_tag="VGE48_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1042162.1"
                     /translation="MQNLRPFQIIVMAIFGVIALLGIALFASFQGFSSGVPQVGSVTI
                     WGTLPANEMNMALGEYKQAHQEYGKVSYAQRPQETFDRDLAEAIASGRGPDLVVISQE
                     DLASAQSKLGMIPSSTLSERDFRSTFLPIGELFLTTGGTYGIPFVVDPLVLYYNRPIL
                     ASAGATQPPATWEAVSGLAGTVNREGEGQTVSRSLIALGTYENIDNARAIISLLFMQA
                     GYQITTRTTNGLVSDLDTMKGTNYGIRPTESALNFYTEFANPTKTVYSWNRSLPQSRD
                     AFVAGDLALYLGFASERKALSAANPNLDFDMAPVPSPGTSQTRVTYGKAYAFAIPRTS
                     ANMEGAVRVARALSDAKSVSGFARTLGMAPAARAALAPAKDDLFEPVFYPEALVTRGW
                     LSPAPADIDTIFATMIRNVASGRESVNNAISTASQSINAAY"
     gene            6519..6890
                     /locus_tag="VGE48_01945"
     CDS             6519..6890
                     /locus_tag="VGE48_01945"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042163.1"
                     /translation="MSKILAILTLVLMDVSRVAAQTTPGTGSGTETVSSGGLKNPLSV
                     GSLPEFLTAILTGIVRIGTIVLIMMLVYVGFLFVTAQGKAEELSKAKSALVWTVIGGL
                     ILLGATAIQQVIQGTVNTVLN"
     gene            6893..7198
                     /locus_tag="VGE48_01950"
     CDS             6893..7198
                     /locus_tag="VGE48_01950"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042164.1"
                     /translation="MHRLLPLSLLALPVVAHAASLKEFTQSLVGIVNTAVMPLLYALA
                     ILFFFIGMTRFLFMNGEENRQKGKMFMLWSVIGLVVMFSVWGIVRLFLTLIPGALSV"
     gene            7238..7660
                     /locus_tag="VGE48_01955"
     CDS             7238..7660
                     /locus_tag="VGE48_01955"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY1042165.1"
                     /translation="MILRTAFFSFALFLLSASSVYAQYEISGNTIPSMLQSITRFIGG
                     VLIPLVFALAFLAFIWGLFLYFIAGGADEEKRAQGKQLAIWGVVGFFVMVSVWGLVNI
                     IRGTFNLNNQVPALPGFTPGQGSGNTPNPSINDDETFF"
     gene            7755..8120
                     /locus_tag="VGE48_01960"
     CDS             7755..8120
                     /locus_tag="VGE48_01960"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY1042166.1"
                     /translation="MKKLGYLAAALAFPLFVSAQVTATNAGTIGQGIISLINNVLVPL
                     VFAIAFIVFIWGVFQYFIMGGADEEKRENGKSLMLWGIIGFFVMVSVWGLVNILRGTF
                     QTTNTAPPVGDVTPSVIVN"
     gene            8180..8428
                     /locus_tag="VGE48_01965"
     CDS             8180..8428
                     /locus_tag="VGE48_01965"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042167.1"
                     /translation="MNGFNDFITRVQDAILTPIITLLALGAFVVFMWGIVVFIAGAED
                     EEKRRTGQQHMIWGIIGLVIIFGANAIVSILKATVGGS"
     gene            complement(9095..9175)
                     /locus_tag="VGE48_01970"
     tRNA            complement(9095..9175)
                     /locus_tag="VGE48_01970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9140..9142),aa:Leu,seq:tag)
     gene            complement(9230..9302)
                     /locus_tag="VGE48_01975"
     tRNA            complement(9230..9302)
                     /locus_tag="VGE48_01975"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9267..9269),aa:Lys,seq:ctt)
BASE COUNT         2079 a         2539 c         2668 g         2032 t
ORIGIN      
        1 atctcgccgc ccccttcacc gtcaggtgcc ggccgaatgg ccgcggtggc cgtcggcgcc
       61 gcccaggtcg atcgagtcgt tcacgttgtc cgagatcatg tcgctgtcca ggccgtcggt
      121 ggccgtcagg gcgatgttgg cgtcggtacg gtgccggtcg atgtggccgg tgtcgtcgat
      181 cagggcgccg gaggtcagct ggctctggtt cacgaagctg gcgtggccgg cgtcgggcag
      241 cggatccagc ggcgacgtgg cggcggcctg ggacgacatg gcggcgagcg cgagagcggc
      301 ggccgaagcc gcgatcaaag cggtgattct cactggtggt ctccttctgt cagaggtctg
      361 tggtccatcc cggtggtcgg gcaactcaca cgaccaggaa aaaatacccc aatcttcatc
      421 tattgtcaat ggcctgtgga tatgtcgtaa tgataaatag gaaagacctg ccaggaggca
      481 ggccggaaaa ggagctggac cgcaggtggt cggtaacccg gagaaaaccc ggcacttggt
      541 cctgcggacc agctcccgtg ccgcgcatca ccgcgcacac aggtatcgta cggccttagt
      601 gccaaatctt ctcaaagcgg cctgtgcata ggctcctaat tgctatcaaa gcacttatga
      661 ttcctgtata atgaagggaa catggttatc acccatcacg gcgggcagtg cttcaaagtg
      721 acgctcggag accgcagttt ggtcttcgac ccgatcgcaa agggggcttc gctccccgca
      781 gtgcgcttcg gtgcagatat tgcgctcgtc tcacgaaagc acaaggacat gaacggcgtg
      841 gccgaggtct cgttcggcgg cactgagccg tttgttattg atggaccagg agaatacgag
      901 gtagcaggcg tcacggtcca agggttcctc acgaagagtc agtacggcct tccgaaggga
      961 gagacggatg ctgtgaacac ggtatacgcc gtatcgctcg agggcatgac tctcgtgcac
     1021 ctcggagcgc tctcggatcc catgctctcg catgacgcgc gcgaggcaat cgatgacatc
     1081 gacgtgctgt tcgtgccggt aggcggcgat ggcgtactga acgccgctga agcggcaagg
     1141 cttgcggtga cacttgaacc caaggtagtt atcccgatgc actgggatgg tatgggggag
     1201 ccgaaggcac ttgagagctt ccttaaggaa agtggcaagg agagcgagaa ggtagacaag
     1261 cttacactta aaaagaagga tgctgctgat aaggacggtg ctatcatcgt cataaccgca
     1321 tagcctatgg cactagacaa tctcaaagga cacattgagc gccttcaaga gaagccggag
     1381 cacgtgcgtc accagatcgc tatgggcgtt gcggttggtg taaccgcgct tgtggcaacc
     1441 ggatgggcag ttgcacttgg tacatcggga gcgcttgcac ttagtaatcc cgagcctgtt
     1501 gagggcgacg gacgtgattc gcttattgaa tcgttcgaag aacccgcaaa cgcgttcaca
     1561 aaccttatgg gtgctgcagg tgctgcgttt aatgctacga gcactgaggc tgcgcttaag
     1621 gttatcgaga cccggacctc gtcaaccctt gagggacgcg cacagcccgc aaacaatact
     1681 gagaagaccg tcattccgtt ctagtttcca tgcctaagaa agctctaaaa gaagaagcac
     1741 cgatcgagcc gcgtatcaac gtggtggatc agtccattac gagcgagatg cgctcgtcct
     1801 accttgatta cgcgatgtcc gtcattacat cgcgtgccct tcctgacgtg cgagacggat
     1861 tgaagccagt gcatcgccgc atcctctacg cgatgcgtca gaccggactc acgccttcag
     1921 ctaagttcag gaagtctgca acggtagtgg gagaggtgct cggttcctac catccgcacg
     1981 gcgacacgtc cgtgtacgac gcgatggtga agctcgccca ggacttcgcg acgcgctatc
     2041 tcctcgtgca gggtcagggc aacttcggtt cggtagacgg cgatccacca gcagcatacc
     2101 gctacaccga ggctaagatg tcgcgccttg cagacgagat gctcaaggat ctcgagaagg
     2161 agactgtgga ctggaatcca aacttcgacg gaagcaagca ggagccgagc gttcttccgg
     2221 ctgcggttcc gaatcttctc cttaacggca cgctcggtat cgcggttggt atggcgacga
     2281 acatcccgcc acacaacctg cgtgaagtag tggacgcaac cacgcacttg atcgataacc
     2341 cggacgcgtc cgtggacgac cttctacagc atgtgaaggg tcctgatttc ccgcttggcg
     2401 cgatcgcctt caaccaggct gacatcaaac acgcgtacac ctcaggacgc ggcggcgtgg
     2461 tggtgcgtgg tgaggccgag atcgtcgagg aaaaaaaggg aaccaacatc gtcatcacat
     2521 cgattccgta tcgcgtgaac aaggctgatc tcatcacgcg cattgcggac ctcgtgcgcg
     2581 acaagaagct tgagggcgtg aaggacctgc gcgacgagtc cgctgcggat atacgtgtcg
     2641 tggtggagct caagggtact gcgcatccgc aggcggtttt gaataatctc tacaaataca
     2701 ccgagctcga atcgacgttc cactacaaca tgctcgcatt ggtggacggc gtgccgcaga
     2761 ccctttctct taagggcatg ctcgagtctt ttatcctcca tcgccaggag gttgttaagc
     2821 gtcgcaccga gtacgagctt cgtaaggcag aggaacgtga gcatatcctc cttggtctct
     2881 caaaggcact cgatcacatc gacgaagtaa tcaagactat ccgtgcctcc aaagacgtgc
     2941 caagcgcgtc cgcagcgctc cagaagaagt tcgccttctc acagatccag gcagcggcca
     3001 ttcttgagat gcgtctccag aagcttgcag cgcttgagcg ccagaagatc gaggacgagc
     3061 tcgcagagat tcaggaactt atcaagcgtc tcaagaagat cctctcgagc gcgaagaaca
     3121 tcctcgcggt tgtgcgtgag gagcttgtgg ctatcggcga gaagtacggc gacgatcgcc
     3181 gcacgaagat cgtgaagggt ggcgtgaaga acatctccgt tgaggatctc gttccggacg
     3241 aggagtccgt actcgtgctt accgcaggcg gttacgtgaa gcgtacgaac ccagacgagt
     3301 acaaggtgca gaagcgcggt ggcgttggag tgatcgacat gtctacgaag gaagaggacg
     3361 tggtaacgca cttcgtgaaa gcaagcgcgc acgcagagct cctgttcttc acggacttcg
     3421 gcaaggcgta ccaactccgc atgttcgatc ttccggaagg aaagcgcgcg accaagggca
     3481 agtcgataat gaacttcctt ccgctcgcag gcgaggaacg tgtcacgtcg gtgcttgcga
     3541 tcccgaaggg caaggagcgc gatgggcttt ccgtattcct cgtgactgaa gcaggaacta
     3601 tcaagaaagt ggaagcgaag tcgttcgcag atgtgcgccg atccggcatc atcgcaattg
     3661 cgcttaaggg tggcgacaag ctcgtttcag cgcatcttgc agcagcggga gactcggtat
     3721 cgatcgtgac tgcggaagga cagtcgatcc gtttcgatga ggcagagaca cgtgccatgg
     3781 ggcgcacggc tgcgggcgtt cgcggcatga atgtgaagaa aggagaccgc gtggtctcgg
     3841 cagaagtagt gccaggcgag gcaggggaca acgcggcgct cctcgtcatt atggacaagg
     3901 gctacggcaa gaaaacctcg ctttctgagt acaaggtgca gggtcgtggc ggctcaggca
     3961 tcaagaccgc agacgtaacc gcaaagaccg gaaagatcat tggtgcaaag gtattgatcg
     4021 gtgacgccga gaaggacgac gagctcgtcg tgatctcaaa gaaaggacag gttatccgtt
     4081 gctcggcagg agagattccg agtctctcac gcgcgacgca gggcgtgcgc attatgaaga
     4141 tgcgcgaagg agacaatatc gcgtcgatgg tggcgcttta aacatataac tgcaatatct
     4201 atgggacttg aaggaaggca gcagttcgga ccggatgcgc ttaggagagt ccctgacaaa
     4261 actgagggta tcaatcctaa taccttcgag aagcggtcgc tcggcgaacg gatggcactc
     4321 accggcgagg agtcacaggc ataccaaagg atgctcatgg caggctcgca aagtgagcta
     4381 cgtgcggcta tcgacgcgtt caacgccatc agcacaccga aggtcgcaag cgagctcgga
     4441 ttctcgctcg acgacatgca aatcatcgac gagcctgtcg gtatccgtcc gcatgacctc
     4501 cctctggatg ctgaggtagt agctacccgt gacatgcgag agggtgtact ccgattcagg
     4561 atcaaggaca ggtagacata tatatctgca gcaaatacgc aaagggcgcg ccaaagacgc
     4621 gccctttgcg tttgttgatt gccacagcct aaggtgcgcc tgcttgctat actttgccta
     4681 tgaatttcag caatccgcag agcaacgtgc tgcagctcgg gctccgcgaa ggaatgaagg
     4741 ttggagactt gggtgtcggc tcaggacatt atgcagcaag cgctgcagca atcgttggtc
     4801 ctgagggacg tgtgtatgcg gttgatatcc aagaagacat cctgaagcat gtaaaggatc
     4861 atgcagccgg taagggtttg cgaaatgttg agaccatctg gggcaacttc gagaagctcg
     4921 gtggtacgaa gctgcgcgat gcttcgttag acgccgttat tctctcaaac accctcttcc
     4981 agctcgatca ccgagagggg gctgtagcgg aaattaagcg catccttaag accggcggac
     5041 gtctccttgt cattgattgg gcgggcgcgt acggaggtat gggtccgctt ccgcatcgcg
     5101 tcgtcacaga acatgttgcg gaagagcttt tcatcagcgc cgggttccat aaggtgaagg
     5161 acttcagagc gggtccgcac cattattcga tcgtattcac cgcgccgtag cttatgcaaa
     5221 acctccgccc gttccagatc atcgtgatgg cgatatttgg tgttatcgcc ctgcttggaa
     5281 tcgcactatt tgcaagcttc cagggattct cttctggtgt tccgcaggtg ggttcggtga
     5341 ccatctgggg aaccctgccg gcaaacgaaa tgaacatggc gcttggcgag tacaagcagg
     5401 cgcatcagga atacggaaag gtctcctatg cgcagcgtcc acaagagacc ttcgatcgcg
     5461 atctcgccga agcaattgcg tctgggcggg gtcctgacct cgtcgtcatc tctcaagaag
     5521 accttgcatc ggcgcagtca aagctcggta tgattccatc aagcacgctc tcggaacgag
     5581 attttcgcag caccttcctt ccgataggtg agcttttcct tacgacgggt ggcacctatg
     5641 gcatcccgtt tgtcgttgac ccgctcgttc tttattacaa tcgtcccatt cttgcgtctg
     5701 cgggcgctac acagccgcct gcaacctggg aagcggtctc gggacttgcc ggcacggtga
     5761 atcgggaagg cgagggccag acggtgtccc gaagtcttat cgcgcttgga acctacgaaa
     5821 atattgataa cgcgcgagcc atcatctctc tcctcttcat gcaggcgggg tatcagatca
     5881 ccacaagaac caccaatggg cttgtgtcag atctcgatac gatgaagggc acgaactacg
     5941 gtattcgtcc aacggaaagt gcactgaatt tctacaccga gttcgcaaac ccaacgaaaa
     6001 ccgtgtactc ctggaaccgt tccttgccgc agagcagaga tgcgttcgtt gcaggggacc
     6061 tcgcactgta tcttgggttc gcatctgagc gtaaggcgct ttctgcggca aacccaaatc
     6121 ttgatttcga catggcaccc gtcccgtctc cgggaacaag ccagacacgc gtaacgtatg
     6181 gcaaggcgta cgcgtttgcc atacctcgta cgagcgcgaa tatggagggc gcggtgcgtg
     6241 tggctcgtgc attgtctgat gcgaaaagcg tgtcaggctt tgcgcgcacg ctcggtatgg
     6301 cccctgcagc acgggctgca cttgcgcctg cgaaggacga tctctttgaa cccgtctttt
     6361 atccggaggc tcttgttacg cgcggctggc tctccccagc acctgcagat atcgacacga
     6421 tctttgctac catgattcgt aacgtggcga gtggtaggga atcggtgaac aacgcgatct
     6481 caaccgcaag ccagtccatt aacgccgcct actagcctat gagcaagatt ctcgccatcc
     6541 taacgctcgt cctcatggac gtgagccgtg ttgctgcgca gactaccccg ggtaccggtt
     6601 ctggcacaga gacggtgtcc tcaggaggac ttaagaatcc tttgtccgtt ggatctctgc
     6661 ctgaatttct caccgcgata ctcacaggca tagtccgcat cggcaccatc gtgcttatca
     6721 tgatgctcgt ctacgtcggt tttctgttcg taacagcgca gggtaaggct gaagagcttt
     6781 caaaagctaa gagcgccctc gtatggaccg tgatcggcgg actcatcctt cttggcgcta
     6841 ccgcaattca gcaggtcatt cagggaaccg taaatacggt tctgaactaa ccatgcatcg
     6901 cctcttacct ctttctctgc ttgcactgcc ggttgtcgcg cacgcggcca gcttgaaaga
     6961 attcacacag tccctggtgg gtatcgtgaa cacggcggta atgccgctct tgtacgcact
     7021 cgccatcctc tttttcttta tcggtatgac ccggttcctc tttatgaatg gagaggagaa
     7081 ccgccaaaag gggaagatgt tcatgctctg gagcgtgatc ggactggtgg ttatgttttc
     7141 ggtgtgggga atcgtgcgac tgttcctcac ccttattccg ggggccctta gtgtataacc
     7201 ctcgttgtac gggcagatat tggtatcctt aaggcatatg attctaagaa ctgcattctt
     7261 ctcgttcgcg ctcttcctgc tttcagcttc gtctgtgtac gcacagtatg aaatatcggg
     7321 taataccatc ccgtcgatgc tgcagagcat tacgcgcttc atcgggggcg tacttattcc
     7381 gcttgtattt gcgctcgcgt tcctcgcgtt tatttggggc ctcttcctct acttcattgc
     7441 tggcggtgca gacgaggaaa agcgcgcaca gggaaagcag cttgcgatct ggggcgtggt
     7501 tggcttcttc gtgatggtat ctgtttgggg cctcgtgaac atcattaggg gcacgtttaa
     7561 cctgaataac caggtaccgg ccctcccagg cttcacaccg ggtcagggtt ctggcaatac
     7621 gccgaacccg tcaatcaacg acgacgagac attcttctaa aaagccgctg tccacacgga
     7681 tttcagggct ctattgacgc ttcatcattc cagactatcc ttaaaggtat acgcgttata
     7741 cgtaatcatt atctatgaag aaactcggtt atcttgctgc cgctctcgcg ttcccactct
     7801 tcgtgtccgc acaggtcacg gctacgaacg caggtaccat cggtcagggc atcatcagcc
     7861 ttatcaacaa cgtgctcgtg ccgctcgtat ttgcgatcgc cttcatcgtg ttcatctggg
     7921 gcgtgttcca gtacttcatc atgggcggtg cagacgagga gaagcgcgag aatggaaaga
     7981 gccttatgct ctggggcatc atcggcttct tcgtgatggt atctgtttgg ggtctcgtga
     8041 acattcttcg cggcaccttc cagaccacca acaccgcgcc tccggtcggc gacgtgaccc
     8101 cgtcggtcat cgttaactaa tcctccatcg gcagcacaag agcccgcgcg aatgcgcggg
     8161 ctcttgctat acttacgcca tgaacggatt caacgacttc atcacgagag tgcaggacgc
     8221 gatactcact cctattatta cgttgctcgc gctcggagca ttcgtagtgt tcatgtgggg
     8281 cattgtcgta tttatcgcgg gcgctgaaga cgaggagaag cggagaacgg ggcagcagca
     8341 tatgatttgg ggtatcatcg gcctcgttat tatcttcggt gcaaatgcga tcgtctccat
     8401 cctcaaggcc acggtaggag gaagttagta cgaaaagagc ccgacctatt cagtcgggct
     8461 ccgggcactc agggcccttc gtaggatcac agggaaccag ccagtgcgcc acatagactt
     8521 cgttctcggt tcgagaacgg acttggtagt acgcgtgctc agcacacgaa gtcggggtcc
     8581 acaccgtagg gatcgagcca tctgagagct tgcaccgata ttcgtacggc accttactct
     8641 cgtctttctc agtgctcaga cacgactcga gatcttcctt tgcgacctca cagttcattg
     8701 cccgatatcc gcgagggaca aacacccatt cctctggcca gccatccttg tttggaggcg
     8761 cgaccagcag ggaccgtacg ttgactaccg gttgcggagt aatcgagtct cctctcagtg
     8821 ttccttgtgg tgccatggcc acccggtttg cgtatgagtt gcccggtcga ggagtctgat
     8881 caagaagcac ggcaatcgcg cgcaacacaa tcagaatgat cacgacccag aaaacaaaac
     8941 caaaccagct gtcccaccat ttgcgcttct taggcgccgc tgcatgacca cccatgggtc
     9001 gccctccaaa cgattagatg tgtagcaggt tcgaaatcaa ggacctgcat tcctaacgtg
     9061 agtaaacagt attttatatg gaaagtcaaa tgtgtgctgg agagaggact cgaacctcca
     9121 cccccgaagg gacctgctcc taaggcaggc gcgtatacca tttcgccact ccagcgtatc
     9181 cgtatcctaa cagaaaaaga gagatccgaa gacctctcct tttctctagt ccgcccactt
     9241 ggactcgaac caaggaccaa tcgcttaaga ggcgagtgct ctaccagctg agctataggc
     9301 ggatacaggc gcattcta
//
LOCUS       DASWXU010000055         6961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_503851, whole genome shotgun sequence.
ACCESSION   DASWXU010000055 DASWXU010000000
VERSION     DASWXU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503851"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..137)
                     /locus_tag="VGE48_01980"
     tRNA            complement(63..137)
                     /locus_tag="VGE48_01980"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Cys,seq:gca)
     gene            complement(172..516)
                     /locus_tag="VGE48_01985"
     CDS             complement(172..516)
                     /locus_tag="VGE48_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEY1042168.1"
                     /translation="MSARDDRAVSILLESAGRYIAREAGRSTLITPTRATFSRDRKNA
                     TIYVSVFPEGQDGKALEFLMRHKDLFRNELKKTTRFARLPFIRFELDKGERARQHLDE
                     VTRDLEIPEEDA"
     gene            complement(518..2008)
                     /gene="infB"
                     /locus_tag="VGE48_01990"
     CDS             complement(518..2008)
                     /gene="infB"
                     /locus_tag="VGE48_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY1042169.1"
                     /translation="MARSSRPPIVAVMGHIDHGKSSLLDYIRKANVTAGEAGGITQHV
                     SAYEAEHEHEGSVRKITFLDTPGHEAFRALRARGAQAADIAILVIAAEEGVKPQTMEA
                     FEAISEAGTPLVVAFTKIDKPGADVEKAKISALEAGIYLEGLGGSIAYTGVSSKSGEG
                     IPELLDLVLLTADLAELTADPELPAEGFVLESSQDPKRGLSATLIMKDGTLPVGAFAI
                     AGAALAPVRFIEDFAGKRITAALPSQPIRISGWNQLPPAGTPFSSASSKKDAEKLALE
                     GAAASAAPVRAAVKPANAEHAVLPIVLKADVTGSILAIKHELAKIAHERVTISIIQEG
                     IGAVSEGDVKTAAAANGLIIGFNVGVDSVARDSAERMNVSIDTYTIIYELKERVETLV
                     KERAPRVTIEEILGEAKVLKAFNTAGTKQVLGARWNSGVLSVGDSVKIDRRGLHIGNA
                     KLTNLQVARADVNVIKDEGEFGLQIEGKYEAAPGDTLIAFRMTETQ"
     gene            2077..3228
                     /locus_tag="VGE48_01995"
     CDS             2077..3228
                     /locus_tag="VGE48_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY1042170.1"
                     /translation="MRRTYLDFAAATPVSKEALRAYQKALTVYGNPSSPHTEGRAARE
                     ILEDARVRIARLASVKSDAVIFTSGATEANALVLLGYMRSLAGDREPSSVHCLYLPSA
                     HVSTRGAMEILKVEGFSIEPLRLKDGTIDLVALKSQIRPETALIALEAVCGETGTVFA
                     IRDVRRALPDGSRIRIHVDASQLPRVGPFELTRLGADTLSLDAQKVGGVRGTGVLIVP
                     RSMHIAPLSIAGGQERGLRPGTESHAGAAAFAIALEKAHETRDTFRMRAQRDREMITE
                     TLEKEIPSILFNEGKDQVPHILNISLPTYDTDYLQALLDEAGFAVSTRSACATDAEGS
                     EAVLALTGDQERAASTLRISWGMETSSQDLKRLAAALVQAVRFVDRGTV"
     gene            3238..3981
                     /locus_tag="VGE48_02000"
     CDS             3238..3981
                     /locus_tag="VGE48_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042171.1"
                     /translation="MNIEDLNKTQLLLLTILVNFVTAIATAVMTVSLLDQAPPTVTQT
                     VNRIVERTVETAAETTPLPNILPPKETVVIRDEDLLSGAIRADVSRRVTIHKGATTTP
                     AIAIGTYLPDKRAVVTATGASLPKEATVVFANGASAEASLSKGGATLTIYGFSDTAGL
                     PNVAIPDIVASKDLRQGQTLVSLTGGGAAVSGIISIVEEAGIRANVTGVPAGAAAVNI
                     EGDLIGIATSIPGLFAPAERINVLLAEGA"
     gene            4028..6706
                     /locus_tag="VGE48_02005"
     CDS             4028..6706
                     /locus_tag="VGE48_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1042172.1"
                     /translation="MQPFRNFTTKAKEAIRKAHELAIERGQNHVSPLHLLTALIHQEE
                     SLVFSVLDRMDIDTILLSDTLLETLEIPEASSTLSPSYNLYLTPDLAQALEAAAKIAA
                     RMDDAFVGCEHLFVSILEHPGPAEDILARFRIDEETVLQILKDLKADKDGDAAEPKKF
                     RALSKYTRNLTRLASENALDPVIGRDQEIGRVIQILSRRTKNNPVLIGEPGTGKTTIA
                     EGLALRIAQNDVPESLKGKEILSLDLGLMIAGTKYRGEFEERMKAVMKEVEKSEGGVI
                     LFVDELHTIVGAGAADGALDASNMLKPALARGEMRMIGATTLKEYQQHIEKDGALTRR
                     FQPVYVEEPSIEDATAILRGLRDKYELYHGVRITDGAIVSAVELSARYITDRFLPDKA
                     VDLIDEAASGLRIALENKPPQLEETDRRIRRLEIEREALKKDEESENAKEIAERVSGI
                     DTEIADLREKTSELELKWKNEKEVLESIRSLKAELEKARVEAENAEAMADLGTAAEIR
                     YGRMPSLQKDLETKTKRLKTLQKSRRILHEEVTEQEIAGVVARWTGIPVAKMLEEEAA
                     KLARMEAALKEVVVGQDRAVKAISDAVKRSRVGISDPNRPIGSFLFLGPTGVGKTELS
                     RRLAEFMFNDKESLVRVDMSEFMEKHSVSKLIGAPPGYVGHEEAGSLTERIRHRPYAV
                     VLFDEIEKAHPEVFNILLQVLDSGHLTDGKGRKVNFKNTIVVLTSNIGAEFMDKAANI
                     GFATSEADETAAQERMKEKVMGALKDHFRPEFLNRLDDIVMFDMLGKDMLMKIVDAQV
                     EEVLARLTLKRISLSLDQGVKEWLAEKGYNPQYGARPLKRAVQDHILTPIASLMVGQG
                     IMEGGSVTVSMKNGEPHFEVRKSAQRAARKPKAVAK"
     gene            6735..6887
                     /gene="rpmG"
                     /locus_tag="VGE48_02010"
     CDS             6735..6887
                     /gene="rpmG"
                     /locus_tag="VGE48_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY1042173.1"
                     /translation="MSQDRLIKLKSASGHVRFSRKNKKKVERKIELKKFDPNTRQREV
                     YKEVKK"
BASE COUNT         1532 a         1911 c         2038 g         1480 t
ORIGIN      
        1 atggcattgc tgcctggggt gtttaagacc cctatgtcta ccattccatc accccggcag
       61 tatggaggcc agaacgggaa tcgaacccgt gcatagcggg tttgcaatcc gcagcgttac
      121 cacttcgcca tctggcccta cgaggataat agcgcgtgag gcgcctctgc gctacgcgtc
      181 ttcctcaggg atctcaagat cccgggtaac ctcatcaagg tgctggcgag cacgttcacc
      241 cttatcaagt tcaaagcgaa tgaatgggag acgtgcgaaa cgggtggtct tcttgagctc
      301 attcctgaag agatccttgt ggcgcatcaa gaactcgagc gccttaccgt cctggccttc
      361 aggaaacacg ctcacataga tcgtggcgtt cttacggtcg cgcgagaagg tagcgcgcgt
      421 gggagtgata agcgtagagc gtcccgcctc acgtgcgatg tagcgccctg cgctctctaa
      481 cagtatggat acggctcggt cgtctcgtgc gctcatgtta ttgggtctcg gtcatacgga
      541 atgcgataag tgtatctccc ggcgctgctt cgtacttgcc ctcgatctgg aggccgaact
      601 cgccttcgtc cttgatgacg ttcacgtccg cgcgcgctac ctggaggttc gtgagcttcg
      661 cgttgccgat atggaggcca cggcggtcta tcttcacgga gtcgccaacc gaaagcacgc
      721 cggagttcca gcgtgcaccg agcacctgtt tggtacccgc cgtattaaac gccttaagca
      781 cctttgcttc gccaaggatc tcttcaatag taacgcgcgg tgcacgttcc tttacgagcg
      841 tttctacgcg ctccttaagc tcatagataa tagtgtaggt gtcgatcgac acattcatgc
      901 gctcagctga atcacgtgca acgctatcaa cgcctacgtt gaagccgatg atgagaccgt
      961 ttgcggctgc ggcggtcttc acgtcgccct cggacaccgc gccgatgcct tcctgaatga
     1021 tgcttatggt cacgcgctca tgcgcgatct ttgcgagctc gtgcttgatc gcgaggatac
     1081 tgcccgtcac gtccgcctta agcacgatag ggagcaccgc gtgttccgcg tttgcaggct
     1141 ttaccgcagc acgtaccggt gctgctgatg cggctgctcc ttcaagagca agtttctcag
     1201 cgtctttctt cgaacttgca gaggagaatg gggtgccagc aggagggagc tggttccagc
     1261 ctgaaatgcg aatcggctgc gaaggaagcg ctgccgtgat gcgctttccg gcaaagtctt
     1321 cgatgaagcg aactggagcg agcgcggcac ctgcgatagc aaatgcgcca accggtagcg
     1381 taccgtcctt catgatgagg gtggcagaaa ggccgcgctt cggatcctgg gaagactcaa
     1441 gcacaaagcc ttcggcagga agctctgggt ctgcggtgag ttctgcgagg tctgcagtga
     1501 gaagtacgag atcaagaagc tccgggatac cctcgccaga tttagaagag acaccggtgt
     1561 aagcgataga gccgccgaga ccctcaaggt atatacctgc ctcaagcgcg ctaatctttg
     1621 ccttctcgac atcagcgcct ggcttatcaa tcttcgtgaa tgcgacgacg agtggcgtgc
     1681 ctgcttcgct gatcgcctcg aatgcttcca tggtctgagg cttcacgcct tcttctgcag
     1741 cgatcacgag gatcgcaata tcggcagcct gcgcaccacg agcgcgaagt gcgcggaagg
     1801 cttcgtgtcc tggggtgtcg aggaaggtta tcttgcgaac cgatccttcg tgctcgtgct
     1861 ctgcctcgta tgcggatacg tgctgcgtga tgccacccgc ctcgccagca gttacgttcg
     1921 ccttacggat gtagtccagc aaggaggact tgccgtgatc gatgtgaccc ataacggcca
     1981 cgatgggtgg tcgggagctt cgcgccataa ttgcaaggga ttatctcaca aaatacgcct
     2041 tttaacaaac gggcgttgtc ggcgtagcat ggacgcatga gacgcaccta tcttgacttt
     2101 gctgcagcga cgcccgtatc caaggaggcg cttcgagcgt atcagaaagc actcacggtg
     2161 tatggcaatc cgtcatcccc acatacggaa ggaagggcag cgagagagat ccttgaagac
     2221 gcacgggtgc gcattgcacg actcgcgtcc gttaagagcg atgccgtgat cttcacgtcc
     2281 ggagcgaccg aagcaaacgc actggtactt cttggataca tgcgctcgct cgcaggagat
     2341 cgtgaacctt cctccgtgca ctgcctctat cttccgagcg ctcacgtgtc tacacgcggt
     2401 gcaatggaaa tactgaaggt ggaaggattt tccattgagc cactcaggct caaggacgga
     2461 actatcgatc ttgtagcgct caagagccag attcgcccag agaccgcgct tatagcgctt
     2521 gaggcggtgt gcggagagac cgggacagta ttcgcgattc gcgacgtgcg acgggcactt
     2581 cctgatggat cgcgtatacg catccatgta gatgcgagtc agcttcctcg agtgggtccg
     2641 tttgagctca cgcgcctcgg tgcagatacg ctttcgcttg atgcgcagaa ggtgggtggg
     2701 gtgcgtggca ctggcgtcct gattgtgccg agaagcatgc atatcgcacc gcttagtatc
     2761 gccggtggcc aagagcgagg acttcgccct ggaaccgagt ctcacgcggg tgcggcagcg
     2821 tttgctatag cgcttgagaa ggctcacgag acgcgcgata catttcgcat gcgtgcgcaa
     2881 cgggaccggg aaatgataac ggaaacgctc gagaaggaga ttcccagcat tctttttaat
     2941 gaggggaaag accaagtgcc tcatattctg aatatctcgc tcccaacgta cgacaccgat
     3001 taccttcagg cgctccttga cgaagcgggg tttgccgtct caaccagaag tgcctgtgcc
     3061 actgatgccg agggatcaga agcggtgctt gcgcttacag gtgatcagga gcgtgccgct
     3121 tcgacgctac gcatctcctg ggggatggag acctcgtcac aagacctgaa gcggctggcg
     3181 gctgcccttg tccaagcggt tcgtttcgta gaccggggca cggtataatt tctgcatatg
     3241 aacatcgagg acctcaacaa gacccagctc ctgcttctca cgattcttgt gaatttcgtg
     3301 actgccattg cgactgccgt aatgacggta tcgctcctcg atcaggcgcc tcctacggtg
     3361 acacaaacgg tgaatcgcat tgtcgagcgc acagttgaga ccgccgctga gaccacaccg
     3421 ctcccaaata tcctcccgcc aaaagagacg gtggtgatcc gtgatgagga tcttctttca
     3481 ggcgcgatac gcgcggatgt ctcgcgccgt gtgacgattc ataagggagc gaccacaacg
     3541 cctgcgattg caatcggtac gtacctcccg gacaagcgcg cggttgtgac agcaaccgga
     3601 gcgtcgcttc ctaaagaggc aaccgttgtc tttgcaaacg gtgcgagcgc cgaggcctca
     3661 ctttcaaagg gtggtgccac tctcactatc tacggcttct ccgatacggc cggcctgccg
     3721 aacgttgcga ttccagacat tgtcgcatct aaggacctac gacagggcca gactcttgtg
     3781 tcacttaccg gaggaggtgc cgcagtctct ggcataatct ctatcgttga ggaagcgggt
     3841 atccgcgcta atgtgaccgg tgtgccagca ggagcggccg ctgtaaacat agaaggcgac
     3901 cttataggaa tcgccacctc cattccgggc cttttcgcac cggcagagcg tatcaacgtt
     3961 ctccttgcgg aaggtgcgta aggagcagtt cacggtcccg tcattactag tgtaagattc
     4021 tgtacccatg cagccatttc gtaacttcac cacaaaagcc aaggaggcca tcaggaaggc
     4081 gcacgagctc gccatcgagc gcgggcagaa tcacgtgagc cccctacatc tccttacagc
     4141 gctcatacat caggaagaga gtctcgtatt ctcggtgctc gatcggatgg atatcgatac
     4201 gatccttctt tcagatacgc tcctcgagac gctcgagatt ccggaagcgt cttcgacgct
     4261 ctcgccgtcc tataacctgt atctcactcc cgaccttgcg caggcgcttg aggccgcagc
     4321 aaagattgct gcacgcatgg acgatgcgtt tgtagggtgc gagcacctct tcgtctctat
     4381 tctcgaacac ccaggacccg ctgaggacat cctcgcgcgt ttccgcatcg acgaggagac
     4441 cgtgcttcag atcctcaagg atctcaaggc ggacaaggac ggagatgctg cggagccgaa
     4501 gaagtttcgc gcgctttcca aatacacccg caacctcacc aggctcgcaa gcgagaatgc
     4561 gctcgatcca gttattggtc gcgaccagga gatcggccgc gttatccaga tcctgtcgcg
     4621 ccgcacgaag aacaatccgg tcttgatcgg cgaaccggga accggaaaga ctacgatcgc
     4681 tgagggtctt gcgcttcgca tcgcacagaa cgacgttcct gagtccttga agggcaagga
     4741 gattctctcc ttggacctcg gccttatgat cgcgggcaca aagtatcgtg gcgagttcga
     4801 ggagcgtatg aaggcggtca tgaaggaggt ggagaagtcc gaagggggtg taatactctt
     4861 cgtggacgag ctccatacca tcgttggtgc aggcgctgct gacggcgcgc ttgatgcgtc
     4921 caacatgcta aagccagcac ttgcacgcgg cgagatgcgc atgattggtg caactacgct
     4981 caaggaatac cagcaacaca ttgagaagga tggagcgctc acacgtcgct tccagccggt
     5041 atatgttgaa gaaccttcta ttgaggatgc caccgcgatc ctgcgaggcc ttcgcgacaa
     5101 gtacgagctc tatcacggtg tacgcatcac cgacggcgcc atcgtctctg cagtggagct
     5161 ctctgctcgc tacatcactg atcgtttcct tccggacaag gccgtagatc taatcgacga
     5221 agcggcatct ggacttcgca tcgcgcttga aaataagccg ccgcagcttg aagagacgga
     5281 tcgccgtatc cgtcgccttg agatagagcg cgaagcgctc aagaaggacg aggagagtga
     5341 gaacgcaaag gaaatcgccg agcgtgtctc tggaatcgat acagagatcg cggaccttcg
     5401 cgagaagacg agcgagcttg agctcaagtg gaagaacgag aaggaggtgc ttgaatcgat
     5461 ccgttcgctt aaagcggagc ttgagaaggc tcgcgtcgag gctgagaacg cagaagctat
     5521 ggcagacctc ggtacggctg cagagattcg ctacggccgc atgccgagcc tccagaagga
     5581 tcttgagaca aagacgaagc gcctgaagac gctgcagaag tcacgtcgca tcctccacga
     5641 ggaagttacg gagcaggaga ttgcgggcgt ggttgcgcga tggactggta ttccggttgc
     5701 gaagatgctt gaggaagaag cagcgaagct tgcacgcatg gaggctgcgc ttaaggaggt
     5761 ggttgtgggt caggacaggg ccgttaaggc gatctcagac gctgtgaagc gctctcgggt
     5821 gggcatctca gacccgaatc gtccgatcgg ctcgtttttg ttccttggtc ctacgggcgt
     5881 aggtaagacc gagctctcgc gtcgtctcgc ggagttcatg ttcaacgaca aggagtcgct
     5941 cgtgcgcgtt gatatgtccg agttcatgga aaagcattcc gtatcaaagc ttattggtgc
     6001 acctcctgga tacgtagggc atgaggaagc cggatcactc acggagcgca tccgtcatcg
     6061 cccgtacgcc gtagtccttt tcgatgagat cgagaaggcg cacccagagg tgttcaacat
     6121 tctcctccag gtccttgatt ccggacatct taccgacggc aagggtagga aggttaactt
     6181 caagaacact atcgtggttc ttacttcgaa catcggtgca gagttcatgg acaaggcagc
     6241 gaacatcggg tttgcgacaa gcgaggcgga tgagactgcc gcgcaggaac gcatgaagga
     6301 gaaggttatg ggtgcgctta aggatcactt ccgtccggaa ttcttgaacc gacttgatga
     6361 catcgtcatg ttcgacatgc tcggcaagga tatgctcatg aagatcgtag atgcgcaggt
     6421 agaggaggtg ctcgcacgac tcacgcttaa gcgcatctcg ctttcgcttg atcagggtgt
     6481 gaaggagtgg ctcgcggaga aggggtacaa cccgcagtac ggtgcacgac cccttaagcg
     6541 tgcggtccaa gaccacatcc tcaccccgat cgcgtctctt atggtgggtc agggaatcat
     6601 ggagggcggt tctgtaaccg tgtccatgaa gaacggggag ccgcactttg aagtgaggaa
     6661 gtctgcccag cgcgccgcta ggaagcctaa ggccgttgcg aagtagcggg gcccctgcta
     6721 tagtcctcct atctatgtcc caggatcgtc tcatcaagct caagtcggct tccggccacg
     6781 tgcgcttttc ccgcaagaat aagaagaagg tagagcgcaa gatcgagctc aagaagttcg
     6841 atccgaacac ccgccagcgc gaagtctaca aggaggttaa gaaataagag aagccgcccg
     6901 gaagggcggt ttttcgtttg ggtagctcta tgaaaagcgg aaaacccgtc cgtggacggg
     6961 t
//
LOCUS       DASWXU010000056         5850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_44226, whole genome shotgun sequence.
ACCESSION   DASWXU010000056 DASWXU010000000
VERSION     DASWXU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44226"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGE48_02015"
     CDS             <1..234
                     /locus_tag="VGE48_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY1042174.1"
                     /translation="VMYRDFAQRKATGLKLSGEVQNLKDGTVRVVAEGPREKLERYLQ
                     KLRKGSLLARVERVEHEWRPATGDFTRFVIVYA"
     gene            227..904
                     /gene="uppS"
                     /locus_tag="VGE48_02020"
     CDS             227..904
                     /gene="uppS"
                     /locus_tag="VGE48_02020"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY1042175.1"
                     /translation="MPENPKAIAVIMDGNRRWAREQGLPTLAGHEAGARKMSELVAWA
                     REAGIKEVTVYAFSTENWNRSEEEVQGLMALFRRFIRESAQKLASSGVRIRFVGERPR
                     FAEDIQSAMQEVEEKTEEGDVTLILALSYGGRREILAAANQLLAEGKESVSEEEFSEA
                     LWTRGLLDPDILIRTGGEQRLSGFLPWQSVYSELFFTDTKWPDFSKEEFLEILETYTA
                     RERRRGK"
     gene            920..1645
                     /locus_tag="VGE48_02025"
     CDS             920..1645
                     /locus_tag="VGE48_02025"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY1042176.1"
                     /translation="MNPEIIYEDEDLVAIMKPAGVMTHPDGHNTEETASDWFARVYPA
                     SKDVGEMQRLKDGTEIARPGVVHRLDRDTSGVLVFAKTQGSHARLKDAFQNRHAKKTY
                     LALTYGVPKEPKGIIDFAIGRSRKDFRLRSAQPRARGTMRDALTRYEVLSDIGTHALL
                     KAMPETGRTHQIRVHLKAIHHPVVSDPLYAPGRPNDFKIPRLALHAYQLQLPGKPLLT
                     APVPEDIAPAFRRFPGGEQFIAP"
     gene            1673..2197
                     /gene="rplS"
                     /locus_tag="VGE48_02030"
     CDS             1673..2197
                     /gene="rplS"
                     /locus_tag="VGE48_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY1042177.1"
                     /translation="MNSVITPVKTEERVKLGIKPGDTVRVHQKVVEKGKTRLQVFEGL
                     VLAVKHGTEPGATFTVRATMSGIGVEKIFPLYSPIIDKIEITKRSKVRRAKLYFIREK
                     VAREVRRQLRNMRMVKLSTEDFKEEEKPEEPAAPAEDVTVEVDTVPEGDQDVVTPEEV
                     AAEEAREEKKNEEA"
     gene            2269..2353
                     /locus_tag="VGE48_02035"
     tRNA            2269..2353
                     /locus_tag="VGE48_02035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2303..2305,aa:Leu,seq:gag)
     gene            2424..2496
                     /locus_tag="VGE48_02040"
     tRNA            2424..2496
                     /locus_tag="VGE48_02040"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2457..2459,aa:His,seq:gtg)
     gene            2506..2904
                     /gene="mscL"
                     /locus_tag="VGE48_02045"
     CDS             2506..2904
                     /gene="mscL"
                     /locus_tag="VGE48_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY1042178.1"
                     /translation="MKSFLAEFKKFALRGNAIDLAVGVVIGAAFTAITNSLVTNIITP
                     PLSLLTRGINFADLAFQIPNTDASIQYGLFIQAVITFFITAFALFLLVKFINHLTTIA
                     RREQEEGKTEPAAKSPELQVLEEIRDTLKQ"
     gene            2906..3547
                     /locus_tag="VGE48_02050"
     CDS             2906..3547
                     /locus_tag="VGE48_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042179.1"
                     /translation="MRELLAGVVILFVVGVGAFLYRNTMERPGVIMPEVACTMEAKIC
                     PDGTSVGRMGPTCAFAPCAFPNVEIESANIGFVAPEGYKVAASEDALVRLEKPSAGGW
                     PPHVITVYRYPIAEGETADEILLRVTTYQPSGEQATDFERFSTVSAGGKTYRETTIER
                     FEALIHTSYFLVREGDVLRFDIVEHDVADWMSTTASHTFPEHQALLGLLRSVK"
     gene            3605..4393
                     /locus_tag="VGE48_02055"
     CDS             3605..4393
                     /locus_tag="VGE48_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY1042180.1"
                     /translation="MNQFISELSSNRTARLAAVAVLILLALFLLVSTWDKAFGRDVSD
                     PFNTITVEGTGSSAMVPDTARITFTVMESASQVAAAQEAATAKTDAALAALSEMGIEE
                     RDIKTLSYNVSPKYEYPQPCYGGICTTSAPRIVGYDVSQSVEVKVRETEKAGDVLAAL
                     GGLGVQNISGPEFVVDDETAVKSEARAEAVEEARAKAKELAKQLGVRLGKVVSFSEMA
                     PGDPYYGYGKGGMMMDSAVAQSAPSLPVGENETEITVMITYEIR"
     gene            4512..4700
                     /gene="secE"
                     /locus_tag="VGE48_02060"
     CDS             4512..4700
                     /gene="secE"
                     /locus_tag="VGE48_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY1042181.1"
                     /translation="MKSFFTYLRNVRGELAHVVWPDRKQAILHTFLIVLISAIVAVYL
                     SGIDYVFTGVVNRLIQGY"
     gene            4721..5287
                     /gene="nusG"
                     /locus_tag="VGE48_02065"
     CDS             4721..5287
                     /gene="nusG"
                     /locus_tag="VGE48_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY1042182.1"
                     /translation="MDLMKAPQFEENADQTPRWYTIHTYAGYENAVERNLKQRIESLG
                     MENKIFEVVVPTEKKIRVKAGKRIVEEEKIYPGYILVNMIVDEDSWFVVRNTPRVTGF
                     VGAGNTPVPMEEKEVETLMKRMGAEAPKHRIDLMKDDPVVIADGPFKDLEGKVGEIDE
                     ERGKVKVMVAMFGRETPVELDFLQVRKL"
     gene            5311..5733
                     /gene="rplK"
                     /locus_tag="VGE48_02070"
     CDS             5311..5733
                     /gene="rplK"
                     /locus_tag="VGE48_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY1042183.1"
                     /translation="MAKAIKKIKLQIPGGKATPAPPVGTVLGPAGVNIADFVNKFNEQ
                     TRDQMGVVIPVEMTVYDDRTFTFVLKVSPMSRLILKALGIEKGSSKTPFSKVGTLSRE
                     QVRQIAEQKMPDLSAGSIEAAERIVEGTARSMGVGVAK"
BASE COUNT         1282 a         1642 c         1782 g         1144 t
ORIGIN      
        1 gtcatgtaca gggatttcgc gcagcgaaag gcaaccggcc tcaagctctc aggcgaagta
       61 caaaacctga aagacggtac ggtacgggtc gtagccgagg gtccgcgaga gaagctcgaa
      121 cgataccttc agaagctccg caagggctcg ctccttgcgc gggtcgagag ggtagagcac
      181 gagtggcgtc cggccacagg agacttcacg cgcttcgtga tcgtgtatgc ctgagaatcc
      241 gaaagcgatt gccgtcatca tggacggcaa tcggcggtgg gcgcgcgaac agggactccc
      301 gaccctcgca ggacacgaag ccggtgcgcg gaagatgagt gagcttgtgg cgtgggcacg
      361 cgaggccgga attaaggagg ttacggtata cgcgttctcg accgagaact ggaatcgatc
      421 tgaagaagaa gtgcaaggac ttatggcact cttcaggcgc ttcatacgcg agagtgctca
      481 gaagctcgca tcttcggggg tgcgtatccg gtttgtgggc gagcgaccgc gctttgcaga
      541 agatatacaa agcgcgatgc aagaggtcga agaaaagact gaggagggag acgtcacgct
      601 cattctcgcg ctttcgtatg gaggccgccg cgagatactc gctgcggcga atcagcttct
      661 tgccgaaggg aaggagagcg tctcggaaga agagttctca gaagccttgt ggaccagggg
      721 actgcttgat ccggatatct tgatacgaac cgggggcgaa cagcgcctct ccggcttcct
      781 tccgtggcaa tccgtataca gcgagctctt cttcacggac acgaaatggc cggattttag
      841 caaagaggaa ttcttagaga ttcttgagac ctatacggct cgcgagcgca ggcgggggaa
      901 atagtggtag ggtgtctcca tgaacccgga gatcatctat gaggacgagg atctggttgc
      961 gatcatgaag cctgcgggcg tcatgacgca tccggacggc cataatacgg aagagaccgc
     1021 gagcgactgg ttcgcgcgcg tgtaccctgc gtccaaagac gtaggcgaga tgcagcgcct
     1081 caaggacggg acggagatcg cacgccctgg cgtggtgcat cgccttgatc gcgacacctc
     1141 gggcgtcttg gtctttgcga agacccaagg atcgcatgct cgtctcaagg atgcgttcca
     1201 gaaccggcat gcgaagaaga cctatctcgc cctcacgtac ggcgtaccga aggaaccgaa
     1261 aggcatcatc gacttcgcga tagggaggag ccggaaggac ttcaggctgc gttctgcgca
     1321 gccgcgcgcc cgcggcacca tgcgagacgc gcttacgcgc tacgaggtgc tctctgatat
     1381 cgggacccat gcgctcctga aagcgatgcc ggagaccgga aggacccacc agatccgcgt
     1441 gcaccttaaa gccatacatc atccggtggt atcagatccg ctgtacgctc cgggtcgccc
     1501 gaacgatttc aagatcccgc gcctcgcgct ccatgcgtac cagctccagc ttccagggaa
     1561 gcccctcctt actgccccgg tcccggagga catcgccccg gccttcaggc gattcccggg
     1621 aggcgagcaa tttattgccc cgtaacaggg gctgtggtac agtaccgcgg caatgaattc
     1681 cgtaatcact ccagtaaaga ccgaggagcg cgtgaagctt ggcatcaagc caggcgatac
     1741 cgttcgcgtc caccagaagg tggtcgagaa gggcaagaca cgtctccagg tgttcgaggg
     1801 cctcgtgctc gccgttaagc acggcaccga gccgggtgct accttcacgg tgcgcgctac
     1861 catgtccggc atcggcgtgg agaagatctt cccgctttac agcccgatca tcgacaagat
     1921 cgagatcacg aagcgctcga aggtccgccg cgcgaagctt tacttcatcc gtgagaaggt
     1981 cgcccgagag gtacgccgcc agctccgcaa catgcgcatg gtgaagctct cgaccgagga
     2041 cttcaaggaa gaggagaagc cggaggagcc agctgcccct gcagaggacg tcacggtaga
     2101 ggtagacacc gtaccggagg gcgatcagga cgtagtcact ccggaggagg tcgcagccga
     2161 ggaggcgcgc gaggagaaga agaacgagga ggcgtaagcc ccaaagttga aaatactgga
     2221 aaggcccgct atagtaagcg ggctttcctc gttcgaggtg agacacctgc ccaggtgatg
     2281 aaattggtat acatgcatcc ttgaggtgga tgtgccgcaa ggcgtggagg ttcaagtcct
     2341 ctcctgggca ccaagaaaga tgagccgcag cgatcgctgc ggctcatctt ttttggatta
     2401 ctgtgctata acccttctac atggcggcct tagctcagtt ggtagagcac tcggttgtgg
     2461 tccgagcggt catgggttcg agccccatag gtcgccccta aacatatgaa atccttcctc
     2521 gctgaattca agaagttcgc ccttcgcggc aacgcgatcg accttgcagt gggcgtcgtg
     2581 atcggcgccg cgttcacggc catcacaaac tcgctcgtga cgaacatcat cacaccgccg
     2641 ctgtccttgc ttacgcgcgg catcaacttc gcggacctcg cgttccagat tccgaacacg
     2701 gacgcatcga tccagtacgg cctcttcatc caggcagtga tcacgttctt catcacggcg
     2761 ttcgcgcttt tcctcctcgt gaagttcatc aaccacctca cgacgatcgc acgccgcgaa
     2821 caggaagaag ggaagaccga accggcagcg aagagcccgg agcttcaggt gctcgaggag
     2881 atccgcgaca ccttgaagca gtagcatgag ggagcttctc gccggcgtcg tcatcctctt
     2941 cgtggtcggc gtgggcgcat tcctctatcg caacaccatg gagcgcccgg gtgtgatcat
     3001 gcctgaggtc gcatgcacca tggaagctaa gatctgcccg gacggcacat ctgtcgggag
     3061 gatgggtcca acgtgcgcgt tcgcgccatg cgcgttcccg aacgtagaga tcgagagcgc
     3121 gaacataggg tttgtggcgc ccgagggata caaagtagct gctagcgagg atgcgctcgt
     3181 gcgccttgaa aagccttcgg ccggcgggtg gccgccgcac gtcatcacgg tgtaccgcta
     3241 tccaatcgcg gaaggggaga ctgctgacga gattttgcta cgcgttacta cgtaccagcc
     3301 ttcgggagag caggcgacgg attttgagcg tttctcgacc gtatcagcag gaggaaagac
     3361 ctatcgcgaa actacgatcg agcgcttcga ggctttgata cacacatcct atttcctcgt
     3421 gcgggaagga gatgtgctta ggttcgatat cgtggagcat gatgtggcgg attggatgtc
     3481 taccaccgcg agccacacat ttccagagca ccaggcactc ctcgggctcc ttcgctcagt
     3541 taagtaagaa aagacgggca tgcccgtcgt gctagcataa gggcgaacca ctaacagaat
     3601 attcatgaac cagttcatca gcgagctttc gagcaaccgc accgcccgcc ttgcggctgt
     3661 agcggtcctt atccttcttg cgctcttcct cctcgtctcc acgtgggata aggcgttcgg
     3721 acgcgacgta agcgacccgt tcaacaccat cacggttgag ggtacgggct cttccgctat
     3781 ggtgcctgat accgcacgca tcaccttcac ggtaatggag agcgcgtccc aggtagcagc
     3841 agcgcaggag gcagcgaccg caaagaccga tgcagcactc gccgcgcttt ccgagatggg
     3901 aatcgaggag cgtgacatta agacgctctc gtacaacgta tctccgaagt acgagtaccc
     3961 gcagccgtgc tacggcggca tctgcactac gtccgcaccg cgcatcgttg gatacgacgt
     4021 gtcgcagtcg gttgaggtta aggtccgcga gaccgagaag gcaggtgacg tgctcgccgc
     4081 gctcggcggc ctcggcgtac agaacatctc aggtcctgag ttcgtcgttg acgacgagac
     4141 ggcggtgaag agcgaggctc gcgccgaagc agtagaggag gcgcgcgcga aggcgaaaga
     4201 gctcgcgaag cagctcggcg tccgtcttgg caaggtagtg tccttctccg agatggcccc
     4261 gggtgatccg tactacggct acggcaaggg tggcatgatg atggattcgg cagtcgcgca
     4321 gagtgcaccg tcgctcccgg taggggagaa cgagaccgag atcacggtca tgatcaccta
     4381 cgagatccgc taaggatgac gtgcgaaaag aggccccgct ttgtgcgggg cctctttcgt
     4441 ttgacaccca aaaacctaag tagtatcatg cttttataca aggcccgcgg ggctttttta
     4501 tttggaagac catgaaatca ttcttcacct acctcaggaa cgttcgcggc gagcttgccc
     4561 atgtcgtatg gccggatcgc aagcaggcga tcctccacac ttttctcatc gtcctcattt
     4621 cggcgatcgt ggcggtgtac ctttccggaa tcgactacgt gttcacgggc gtggtgaatc
     4681 gtcttatcca gggctactag ccgcttaacc aaaactcgct atggacctga tgaaagctcc
     4741 tcagttcgaa gagaacgcgg accagacccc gcgctggtac accattcata cctacgcagg
     4801 atacgagaac gctgttgagc gaaacctcaa gcagcgtatc gagtcccttg gcatggagaa
     4861 caagatcttc gaggtggttg ttccaactga gaagaagatc cgcgtgaagg cagggaagcg
     4921 catcgtcgag gaggagaaga tctacccggg ttacattctc gtgaacatga tcgtggacga
     4981 ggattcctgg ttcgtggtgc gcaacactcc gcgcgtaacc ggattcgtag gcgcgggcaa
     5041 caccccggtc ccgatggagg agaaggaggt agagacgctc atgaagcgca tgggcgccga
     5101 ggccccaaag catcgcatcg acctcatgaa ggacgacccg gtagtcatcg cagacggtcc
     5161 attcaaggac cttgagggca aggttggcga gatcgacgag gagcgcggca aggtcaaggt
     5221 catggtggcc atgttcggtc gcgagactcc ggtggagctc gatttcttgc aggttcgcaa
     5281 gctctagcgt agagttctaa tcactatcgt atggcaaagg caatcaagaa aattaagctc
     5341 cagattccgg gtggtaaggc tactccggct cctccggttg gtaccgtgct cggtcctgcc
     5401 ggtgtgaaca tcgctgactt cgttaacaag ttcaacgagc agacccgcga ccagatgggt
     5461 gtcgttatcc cggttgagat gaccgtctac gacgatcgca ccttcacctt tgttctcaag
     5521 gtctccccga tgtcccgtct tatccttaag gcgcttggca tcgagaaggg ttcctcgaag
     5581 accccgttct cgaaggttgg tacgctctcc cgcgagcagg tccgccagat cgcagagcag
     5641 aagatgccgg acctctccgc aggttccatc gaggctgctg agcgcatcgt tgagggtacg
     5701 gcccgctcga tgggcgtagg ggtcgcgaag tagcgcattc cagtttgata agaaaagacc
     5761 tcccgctcgg gaggtctttt cttatttggt cttcttcttg tgccaaggct gcgctttctt
     5821 gatgtcgcgg tcgccccaga gcttcgggag
//
LOCUS       DASWXU010000057         4893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_965059, whole genome shotgun sequence.
ACCESSION   DASWXU010000057 DASWXU010000000
VERSION     DASWXU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965059"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VGE48_02075"
     CDS             complement(<1..227)
                     /locus_tag="VGE48_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042184.1"
                     /translation="MKNLAIIVAAVAIASVPLLSEARTPVMCTMEYAPICGTDVKGAY
                     RTYGNSCTLGAEGGTFKHQGECTEAELSGKGA"
     gene            complement(265..525)
                     /locus_tag="VGE48_02080"
     CDS             complement(265..525)
                     /locus_tag="VGE48_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014875518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY1042185.1"
                     /translation="MKCPIDSSELQMTERQGVEIDYCPQCRGVWLDRGELDKIIERGG
                     VPVQSAPRMPEYPERSYRDDEHSSYGHRKHRRENPLADLFDF"
     gene            complement(538..930)
                     /locus_tag="VGE48_02085"
     CDS             complement(538..930)
                     /locus_tag="VGE48_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1042186.1"
                     /translation="MKGFITDIEKETLENDNFRKVLYTASYMQLVVMSIPAGEDIGEE
                     VHGQDQFIRVEQGSGKAILDDVEHDLRDDTATVIPAGTKHNIVNTGEGALKLYSIYAT
                     PHHEDGTVHKTKADAEHDHEEFLGDTTE"
     gene            complement(1011..1412)
                     /gene="rpsI"
                     /locus_tag="VGE48_02090"
     CDS             complement(1011..1412)
                     /gene="rpsI"
                     /locus_tag="VGE48_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY1042187.1"
                     /translation="MAEQKNYVTAVGRRKTASARVKLTPAKENSVTVNGMSAREYFKT
                     AERESVTGDVFRAGEWKENYAVEAKVEGGGIAAQAEAVRHGIARALTKAEENSRSTVK
                     AAGFLTRDQRSKERKKPGLLKARKRPQWSKR"
     gene            complement(1421..1798)
                     /gene="rplM"
                     /locus_tag="VGE48_02095"
     CDS             complement(1421..1798)
                     /gene="rplM"
                     /locus_tag="VGE48_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY1042188.1"
                     /translation="MTTTKTQNGERTLYTIDAAGQSLGRVASEAASVLLGKKSANYVQ
                     NAVLPVEVAIVNASQLAMTEKRIAGKEYTHYTGYPGGLRITPLARLLERKGVAEALRK
                     AVDGMIPRNKLRKERMKRLTITD"
     gene            complement(1801..2154)
                     /gene="rplQ"
                     /locus_tag="VGE48_02100"
     CDS             complement(1801..2154)
                     /gene="rplQ"
                     /locus_tag="VGE48_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY1042189.1"
                     /translation="MRHHHKGRTFGRTHDQRVALMRSLMRSLIIHERISTTEAKAKEL
                     RPVIEKLVSHARKDTVANRRLVAARLGNDDEATAKLFQTIAPRYAERAGGYTRIVKRA
                     ATATDGRKTAYIAFV"
     gene            complement(2204..3160)
                     /locus_tag="VGE48_02105"
     CDS             complement(2204..3160)
                     /locus_tag="VGE48_02105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY1042190.1"
                     /translation="MEYHITLPSKPRVVSEEGVQGVYEIDSLYPGYGHTLGNSLRRII
                     HASLPGAAITSVKIEGVSHEFATMDGVRESVMEVLLNLKRVHFALHGDEAQTISLSVK
                     GPKTVTAADFKLPSQVEIANPEAHIADISGKISFELEATIERGLGYVPREVLTKEKVD
                     IGTIALDATFTPIRRVNYEVENMRVGDRTDFNRLRILIETNGTITPREALERSIETMI
                     QQLSAVIGFQEAAAEASAPAASMPVAADMPAGKDPAKVKIDELNLGARVETALDEAGI
                     KSVAGLVKKTASQLKELDGIGDKAGADIQDALDQLGLELKGE"
     gene            complement(3204..3818)
                     /gene="rpsD"
                     /locus_tag="VGE48_02110"
     CDS             complement(3204..3818)
                     /gene="rpsD"
                     /locus_tag="VGE48_02110"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY1042191.1"
                     /translation="MKIGPKYKIAKRLGASVFEKTQTQKFALAEERAARAKKGRRSQQ
                     TEYGKQLLEKQKVRVTYGISEKQFSNAVATAMKTHGAPPAQTLHRLLELRLDNVIYRL
                     GLAPTRRAARQMVSHGHITVNGVKTTIPSRAMKVGDRIAVREGSKGLPLFEGFAEKFM
                     ERPTLSSWLSWNPKTMEGGVQEVPTEEAASPAGDLVAVLSFYSR"
     gene            complement(3840..4208)
                     /gene="rpsK"
                     /locus_tag="VGE48_02115"
     CDS             complement(3840..4208)
                     /gene="rpsK"
                     /locus_tag="VGE48_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002827963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY1042192.1"
                     /translation="MSKAVKKRLERGVLHVEATFNNTKAVLTDDKGNAIMSSSAGALG
                     FSGARKSTPYAAAKVGELLGDKALLIGLKEASVVIRGIGAGRESALRAFAGKGIAIRA
                     IKDATPVPHNGPRPPKPRRV"
     gene            complement(4301..4681)
                     /gene="rpsM"
                     /locus_tag="VGE48_02120"
     CDS             complement(4301..4681)
                     /gene="rpsM"
                     /locus_tag="VGE48_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY1042193.1"
                     /translation="MRIAGVTLPDDKQLAYSLPLIFGIGPTRALDILKEAGVEPTRKG
                     SDLSADEEQKIRTLAEGYTIEGELRREIGQNVKRLKDIRSQRGIRHERRLPVRAQRTK
                     TNSRTVRGNTRKTMGSGRRTLEKT"
     gene            complement(4723..4839)
                     /locus_tag="VGE48_02125"
     CDS             complement(4723..4839)
                     /locus_tag="VGE48_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY1042194.1"
                     /translation="MKVRASIKVQQPGDIIVRRRGRLYRINKRNPRRKARQG"
BASE COUNT          939 a         1405 c         1437 g         1112 t
ORIGIN      
        1 gctccttttc ctgagagctc tgcttcggtg cattcgccct ggtgcttgaa tgttccaccc
       61 tctgcgccga gcgtacagct gttcccgtac gtcctgtatg cgcccttcac atccgtgccg
      121 catatcggcg catattccat ggtgcacata acgggcgtgc gagcctcaga caagagcggt
      181 accgaggcta tcgcgactgc tgcgacaatt atcgcgaggt tcttcatctc gcttctgtag
      241 acgcacggag ggcccgttcc ttacctagaa atcaaagaga tcggcgagtg gattctcgcg
      301 gcgatgcttc ctgtgtccgt aggaactgtg ttcgtcgtca cgatatgaac gttccgggta
      361 ctcaggcata cgaggcgctg actgcacagg gacaccgcct cgctcaatga tcttgtcgag
      421 ctcgccgcga tcgagccata cgccgcggca ctgcgggcag tagtcgatct cgactccctg
      481 gcgctcggtc atctgaagct ctgacgaatc gatagggcat ttcatatacg tggtgggtta
      541 ttcggttgta tccccgagga actcctcgtg atcatgctca gcatctgcct tagtcttgtg
      601 caccgtgccg tcctcgtgat gcggggtcgc gtaaatgctg tagagcttga gcgcaccctc
      661 tcccgtattc acgatattgt gtttcgtgcc tgccgggata acggttgcag tatcgtcgcg
      721 aaggtcgtgc tcaacgtcgt cgagtatcgc tttgccgctc ccctgctcga cacggatgaa
      781 ctggtcctgc ccatgtacct cctctccaat gtcctcgccc gcagggatgc tcataactac
      841 gagctgcata taggacgcgg tgtagagtac cttcctgaag ttatcgttct ctagggtctc
      901 cttctcgatg tctgtaatga agcctttcat acatgcattc taccccacat acgctaaacg
      961 cttcatgaaa ggaaaaatcc cgcgaatgcg ggacttctcc ttaagaaatg ctagcgcttg
     1021 gaccactgtg gacgcttgcg ggccttcaaa agacctggct tcttacgctc cttggaacgc
     1081 tggtcgcggg tgaggaagcc tgctgccttt acggtagagc ggctgttctc ttctgctttt
     1141 gtaagggcgc gtgcgatccc gtggcggact gcctcggcct gagccgcgat gccgccgccc
     1201 tcaaccttag cctctaccgc gtagttctcc ttccactcgc ctgcgcggaa tacgtcgccg
     1261 gtgacgctct cgcgctctgc agtcttgaag tactcgcgcg cggacatgcc gttcacggta
     1321 accgagttct ccttcgccgg ggtgagcttc acgcgcgcgg atgcggtctt gcggcgaccg
     1381 acggcggtga cgtagttttt ctgttcagcc ataacgaaga ttaatcggtg atagtaaggc
     1441 gcttcatacg ctccttacgg agcttgttgc gcgggatcat gccgtccact gccttgcgga
     1501 gcgcttcagc aacacccttg cgctcaagaa ggcgcgcgag cggagtgatg cggagaccac
     1561 ctgggtagcc ggtgtagtgc gtgtactcct ttcctgcgat gcgcttctcg gtcatagcga
     1621 gctgagacgc gttcacgatc gcaacctcta ccgggagcac ggcgttctgc acgtagttcg
     1681 cgctcttctt accgaggagg accgaagccg cctcagaagc cacgcggccg agggactgtc
     1741 cggctgcgtc gatggtgtag agcgtgcgct ctccgttctg tgtcttggta gtagtcatgg
     1801 ttatacgaat gcgatgtacg cggtcttgcg gccgtcagtt gcagtagcgg cacgcttcac
     1861 gatgcgggtg tagccaccag cacgctcagc gtagcgaggt gcgatggtct ggaagagctt
     1921 cgcggtagcc tcgtcatcgt tcccaaggcg cgcagctacg aggcggcgat ttgccaccgt
     1981 gtccttacga gcgtgggata cgagcttctc gataaccggg cgaagttcct ttgcctttgc
     2041 ctccgtggtc gagatgcgct cgtgaatgat aagggaacgc atgagggaac gcatgagcgc
     2101 tacgcgctgg tcatgcgtac gtccgaacgt ccttcctttg tgatgatggc gcataaatcc
     2161 tgttttaaca cgattctcga ctggagtcca aatcgcttac ctattactcg cccttgagct
     2221 caagaccaag ctggtcaaga gcatcctgaa tatctgcacc ggccttgtcg ccaataccgt
     2281 cgagctcctt gagctgcgac gcggtcttct ttacgagacc tgcgactgac ttgatgcccg
     2341 cctcatcgag tgcagtctca acgcgtgcgc caagattaag ctcgtcgatc tttaccttcg
     2401 ccggatcctt gcctgctggc atgtcagcgg ctaccggcat ggatgcagcc ggagcggaag
     2461 cctcagcggc tgcttcctgg aaaccaatga ccgcagaaag ctgctggatc atggtctcga
     2521 tcgagcgctc gagcgcctca cgcggggtga tagtgccatt ggtctcaatc aagatgcgga
     2581 gacggttgaa gtccgtacgg tcgcccacgc gcatgttctc tacctcgtag ttaacgcggc
     2641 gaatcggcgt gaaggtcgcg tcaagcgcaa tcgtgccgat atcaaccttt tccttggtaa
     2701 gcacctcgcg cggcacgtag ccgaggccgc gctcaatggt tgcctcaagt tcaaacgaga
     2761 tctttccaga gatgtcagcg atatgcgcct ctgggtttgc aatctcaacc tggctcggaa
     2821 gcttgaagtc cgctgccgtt acggtcttag gacccttaac ggaaagcgag atggtctgcg
     2881 cttcgtcgcc atgaagcgcg aagtgtacgc gcttgaggtt aagaagcacc tccatgacgg
     2941 actcgcgaac gccgtccatg gttgcgaact cgtgagaaac tccctcgatc tttaccgaag
     3001 taatagcggc acccggaagc gacgcatgga tgatgcggcg aagcgagtta ccgagcgtat
     3061 gaccgtaccc tgggtacagc gaatcaatct cgtaaacgcc ctggacgccc tcttcagaga
     3121 cgacacgagg cttagacgga agcgtgatgt ggtattccat gactcaatcg atttagacca
     3181 acaaaatagc tgactactaa agactagcgg ctgtagaacg agagcaccgc gacgagatcg
     3241 cctgcaggtg atgcggcttc ctcggtcggc acctcctgga caccgccctc catcgtctta
     3301 ggattccaag aaagccaaga cgaaagggtt gggcgctcca tgaacttctc cgcgaagccc
     3361 tcgaagagcg ggagaccctt cgaaccctcg cgtaccgcga tgcgatcacc caccttcatc
     3421 gcgcgcgatg ggatggtggt ctttacgccg ttcacggtga tgtgaccatg cgagaccatc
     3481 tgacgagccg cacgacgcgt cggcgcaagg ccgaggcggt agatcacatt gtcgagacga
     3541 agctcaagga ggcggtggag ggtctgtgca ggaggcgcgc catgagtctt cattgcggta
     3601 gctaccgcgt ttgagaactg cttctcggag atgccgtagg taacgcgtac cttctgcttc
     3661 tcaagaagct gcttaccgta ctcggtctgc tgggaacgac gtcccttctt agcacgcgca
     3721 gcacgctcct ctgcgagcgc gaacttctgg gtctgggtct tctcgaagac ggatgcgccg
     3781 aggcgcttcg cgatcttgta cttaggtccg attttcatag gtaagattag gtgagaagac
     3841 tagacgcgac gaggcttagg cggacgcggg ccgttgtgtg gcactggggt cgcgtccttg
     3901 atagcgcgga tagcgatgcc cttgcctgcg aatgcacgaa gcgcggactc gcgaccagca
     3961 ccgatgccgc ggataacaac tgaagcctcc ttgagcccga tgagaagtgc cttgtcgcca
     4021 aggagctcac ccaccttcgc tgctgcgtac ggggtggact tgcgggcgcc ggagaagccg
     4081 agcgcacctg cggaagaaga catgatggca ttacccttgt cgtcggtgag gacagccttc
     4141 gtgttgttga aggtagcctc gacgtgaagc acgccgcgct caaggcgctt cttgacggcc
     4201 ttggacatgg cgcggtcctt gcggccctgg tccatgccct tacctgactt cttgatgatt
     4261 cgtttcttac ccataggaaa ttaatgtaat gcgcagcgga ttatgtcttc tcgagcgtgc
     4321 ggcgaccgct acccatcgtc ttacgggtgt tgccgcgtac ggtacgggag ttggtcttgg
     4381 tacgctgtgc gcgaaccggg aggcgacgtt catggcgaat accgcgctgg gagcggatat
     4441 ccttcaagcg ctttacgttc tggccgatct cacggcgaag ctcgccctcg atggtgtagc
     4501 cctcagcgag cgtacggatc ttctgctcct cgtcagcaga gaggtcgctg cccttacggg
     4561 tcggctcgac gccagcctcc ttgaggatgt cgagggcacg ggtcggacca atgccgaaga
     4621 taagcggcag cgagtacgcg agctgcttgt cgtcagggag cgttacaccg gcgatacgca
     4681 tgggatagaa gaattacgaa tagagtactt aaaaactggc ggctagccct ggcgtgcctt
     4741 gcggcgaggg ttacgcttat tgatgcggta caagcgtccg cgtcggcgga cgatgatgtc
     4801 tccaggctgc tggactttga tggatgcgcg taccttcatc tttacgggtt tgtttagcta
     4861 agccgcctca cgatacgagc acgtcctccg tac
//
LOCUS       DASWXU010000058         6773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_965645, whole genome shotgun sequence.
ACCESSION   DASWXU010000058 DASWXU010000000
VERSION     DASWXU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965645"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3525
                     /gene="rpoC"
                     /locus_tag="VGE48_02130"
     CDS             <1..3525
                     /gene="rpoC"
                     /locus_tag="VGE48_02130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY1042195.1"
                     /translation="PERDYECYCGKYKGIRYKGIVCDKCGVEITRSIVRRERMGHIEL
                     VTPVAHIWFLRSMPSRMALILGVPSADLEKVIYFAGYIVTKVAEDVKKRLLSELENEY
                     KTKHKAANTDAERDSLKERMTAAKNDIESVVVGKVFDEITYHRFSVRYGTLFEAKIGA
                     EAIYDLFRNLKLEDLKASLEERYPKAAAAEREKLAKRISLISAMIASNIRPEWMFMTK
                     IPVIPPALRPMVALEGGRHATSDVNDLYRRVINRNNRLKKLLDIHAPEVILRNEKRIL
                     QEAVDALLDNSIRKGGASGGALTPAQRRPLKSLADYLKGKQGYFRQNLLGKRVDYSGR
                     SVIVVGPDLELDECGLPKHMALELFRPFVIAGLLERELAFNIRGAGRLIEDGVAEVWE
                     ILEEAIQGKHVLLNRAPTLHRQGIQAFRPRLIEGDAIQLHPLVCPAFNADFDGDQMAI
                     HVPLSEEAQWEAANVMSANKNILKPGSGEMIVMTQKPLDIVLGTYWLTKKVDDAKGAG
                     TYFQSPNAAILAHEYGAIDLRAPINVLPVAGKEKYANLGEGTFETTVGRLLFNTVLPS
                     DYPFVNDAISKKVITGIMADCINRYGIDALPGIVNKVKRFGFEYATKSGVTWSLEDVT
                     VPEGKDAIVSEARTKVASIESDYENGLLSEEEKRRMVIEVWHGAKTEVEKLVEDALNP
                     AGSVHDMVRSGARGSIGNLTQMAGMKGLIQNTAGETIEVPIVSSMTEGLNPVEYFMST
                     HGARKGLTDTALGTARAGYLTRRLFDVAQDSIITEEDCGTKRGITINRVSASGIKIDF
                     AETIRGRVLAAEAAGLKKGKYLSAEDAAQIAADESIASVSVRSPMACETRYGICSTCY
                     GMDLSTQQPVDLGEAVGTVAAQAIGEPGTQLTMRTFHAGGTASVGGDITQGLPRIEEV
                     FEKRSPKNAATVSKFDGVVVEIREQNRDKVVVVTPEKGQGKKDGSNTEYVLSYPRQAL
                     VKEGDSVVKGQFLSDGSADLDELFEHAGRAAVQEYIIAETSKIYELQGASVSRKHLEV
                     IVKRMFANVKIVDAGDSDYSVGDVVEHWEFEEEVKAMEAEGKIAPKGKEMVLGIKMSA
                     LSRRSFLSAASFEQTTKILIDASLRGSVDTLRGLKENVILGRLIPAGTGFAGSKKRAA
                     IDQMQEKRAQEMARREAERLAMEEQATA"
     gene            complement(3622..3987)
                     /locus_tag="VGE48_02135"
     CDS             complement(3622..3987)
                     /locus_tag="VGE48_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042196.1"
                     /translation="MTLTEAVRLLLQVIGTITVGSIVASVIRSLNDLRNPNLHPFPSL
                     ARCPICDKRIYVWQKSGYRTFDVPIEGLDPEILESLLIQSTATMLTHMQCEGEPRQQP
                     RHISVFVDAAPRQESRSVH"
     gene            4099..5790
                     /gene="dnaG"
                     /locus_tag="VGE48_02140"
     CDS             4099..5790
                     /gene="dnaG"
                     /locus_tag="VGE48_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY1042197.1"
                     /translation="MDTVEQIKVKLSIVDVVSPYVKLTKSGRNWKGLSPFNKEKTPSF
                     YVNPERGTYYCFSSGQGGDHFEFIQRMEGVDFKGALHILAEKAGVEIVYTGGQSRESK
                     DKKDRLYEAMARAETLYADLLKDGKSVAGSAYAYAKSRGLSEESIASWNLGFAPEAWR
                     MTLEKLQEAGFQNQELIQAGLVKEADEKKGTFYDRFRNRLMFPIRDSAGRTVAFTGRA
                     LDPNDQAKYLNSPETDLYKKSEILFGMDRAKDAIRQRGFALLVEGQMDLLMLHQIGFT
                     NTIALSGTALTPQHLSLIKRYADNLMLALDSDRAGLNASMKNAIAALRAGMRVKAVKL
                     PVGKDPADLAVEDAKDLTKRIADAQPIVEFFLSVLAESEGDQHRLILLAEKTVLPLLA
                     AIESPMEREHFVGIAARALGSTPEAIRAGLVRLPKEAVEAKMETVNLPGKSAPVRTPV
                     QKLGSRLHAIYMQYPDSLVAKRVETEYSRVIGAPFPDEPPPENEIFEAGIDYGEAPGE
                     TDADDLVRAFEKAYIAEQLQAATARLRVAETARDEAAIREAEQECQRLTRLLASF"
     gene            5823..>6773
                     /locus_tag="VGE48_02145"
     CDS             5823..>6773
                     /locus_tag="VGE48_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1042198.1"
                     /translation="MATKKKAAKKPVKKASVKKAVPKKAVKKTAPKKAVKKPVKKAAP
                     KKAVKKAPAKKPAKKEVLKNVVRVAAKGAKAKAEAQKVDKADLLIQKGKERGYITYNE
                     ILKEFPHVEDDIMFLDELYERFTTAGIDILEGGMLADDTDQYLAGRNIKGRDSGAYDS
                     IQIYLREIGQYPLLTASEERELAQRIEKGDDEARNILARSNLRLVVSIAKKYVGRSPD
                     LTLLDLIQEGNLGLFKAVDKFDFKKGFKFSTYATWWIRQAITRALADQSRTIRIPVHM
                     VETIAKYKQVSRRLAQALGRDAQPEEIAIEMGVEVDKIYQI"
BASE COUNT         1537 a         1912 c         2033 g         1291 t
ORIGIN      
        1 cctgagcgcg actacgagtg ttactgcggc aagtacaagg gcatccgcta caagggtatc
       61 gtctgtgaca agtgtggcgt ggagatcacc cgctccatag tgcgccgcga gcgcatgggt
      121 cacatcgagc tcgtgacgcc ggtagcgcac atctggttcc tccgctctat gccttcacgc
      181 atggcgctca tccttggcgt cccatcagcg gacctcgaga aggttattta cttcgcaggc
      241 tacatcgtga ccaaggtcgc agaggacgtg aagaagcgcc tcctttctga gcttgagaac
      301 gagtacaaga ccaagcacaa ggcagcgaac acggacgcgg agcgcgactc tctcaaggag
      361 cgcatgaccg cagcgaagaa tgacatcgag tcggtcgtcg tgggcaaggt gttcgacgag
      421 atcacctacc accgcttctc ggtgcggtac ggcacgctgt ttgaggcgaa gatcggcgca
      481 gaggctatct acgacctctt caggaacctc aagctcgagg acctcaaggc atctcttgag
      541 gagcgttacc cgaaggcagc tgccgctgag cgcgagaagc tcgcaaagcg catctcgctc
      601 atctcggcga tgatcgcgtc gaatatccgc cctgagtgga tgttcatgac gaagatcccg
      661 gttatcccgc cagcgctccg tcctatggtc gcgctcgagg gtggccgtca cgcgacgtct
      721 gacgtgaacg acctctaccg tcgcgtcatc aaccgtaaca accgccttaa gaaactcctc
      781 gacatccacg ctcctgaggt gattctccgt aacgagaagc gcattctcca ggaagcggtc
      841 gacgccttgc ttgataactc catccgtaag ggtggtgcat caggcggtgc gctcacgccg
      901 gcacaacgcc gtccgttgaa gtcgctcgca gactacctta agggtaagca gggctacttc
      961 cgccagaacc ttcttggtaa gcgcgtggac tactcaggcc gttccgtgat cgtggtcggt
     1021 ccggacctcg agcttgatga gtgtggtctt cctaagcaca tggcgctcga gctcttccgt
     1081 ccgttcgtca tcgcaggact tcttgagcgc gagctcgcct tcaacatccg cggtgccggt
     1141 cgtcttattg aggacggcgt tgcagaagtc tgggagatcc tcgaagaggc gatccagggc
     1201 aagcacgtgc ttctcaaccg tgctccgacg ctccaccgcc agggtatcca ggcattccgc
     1261 ccgcgcctta tcgaaggtga cgcgatccag ctccatccgc tcgtatgtcc ggccttcaac
     1321 gccgacttcg acggagacca gatggctatc cacgtgccgc tttcagagga ggcacagtgg
     1381 gaggcagcga acgtcatgtc cgcaaacaag aacatcttga agccaggttc tggcgagatg
     1441 atcgtcatga cgcagaagcc gctcgatatc gtgctcggta cgtactggct cacgaagaag
     1501 gtggacgacg ccaaaggcgc cggcacctac ttccagagtc cgaacgcggc tatcctcgcg
     1561 cacgagtacg gcgctatcga tcttcgcgcg ccgatcaacg tgctcccggt agcaggtaag
     1621 gagaagtacg cgaacctcgg cgagggtacg tttgagacca ccgtgggacg tcttctcttc
     1681 aacaccgtcc ttccgtcgga ctatccgttc gtgaacgacg cgatctcgaa gaaggtgatc
     1741 acgggcatca tggcagactg catcaaccgc tacggcatcg atgcgcttcc gggcatcgtg
     1801 aacaaggtga agcgcttcgg cttcgagtac gcaaccaagt ccggcgtgac ctggtctctt
     1861 gaggacgtga ccgttcctga gggcaaggac gctatcgtgt ccgaggctcg cacgaaggtc
     1921 gcttccatcg agagcgatta cgagaacggt cttctttctg aagaggagaa gcgccgcatg
     1981 gtgatcgagg tctggcacgg cgccaagacc gaggttgaga agctcgttga ggacgcgctc
     2041 aacccggccg gttccgtcca tgacatggtg cgctccggcg cccgcggttc catcggtaac
     2101 ctcacgcaga tggccggtat gaagggtctc attcagaaca ccgccggcga gaccatcgag
     2161 gtgccgattg tgtcctcgat gaccgagggt ctcaacccag ttgagtactt catgtccacc
     2221 cacggtgcac gtaagggtct tacggacacc gcgcttggta cggcacgcgc cggatacctc
     2281 acgcgccgct tgttcgacgt cgcgcaggac tccatcatca cggaagagga ctgtggcacg
     2341 aagcgtggca tcaccatcaa tcgcgtgtct gcgtccggca ttaagatcga cttcgcggag
     2401 accatccgcg gacgcgtgct tgctgcagag gctgccggcc tcaagaaggg taagtacctc
     2461 tccgcagagg acgcagcgca gattgcagcc gacgagtcca tcgcgtccgt atcggtacgc
     2521 tctccgatgg cgtgtgagac gcgctacggc atctgctcca cctgttacgg tatggacctc
     2581 tcaacgcagc agccggttga tctcggcgag gctgtgggta cggtcgccgc acaggctatc
     2641 ggtgagccag gaacccagct cacgatgcgt accttccatg ccggtggtac cgcgtccgta
     2701 ggaggtgaca tcacgcaggg tcttccgcgt atcgaggagg tcttcgagaa gcgctcccca
     2761 aagaacgccg ctaccgtttc caagtttgac ggtgtggtcg tagagatccg cgagcagaac
     2821 cgcgacaagg tcgtcgtagt aactccggag aagggtcagg gcaagaagga cggctcgaac
     2881 accgagtacg tccttagcta cccgcgccag gcactcgtaa aggaaggcga ctccgtcgtg
     2941 aagggtcagt tcctctccga cggttctgcg gacctcgacg agctcttcga gcacgccggc
     3001 cgcgccgcgg tacaggagta catcatcgcg gagaccagta agatctacga gctccaggga
     3061 gcgtccgtat cccgcaagca cctcgaagtc atcgtgaagc gcatgttcgc gaacgtgaag
     3121 atcgtggacg caggcgactc tgactactcg gtaggcgacg ttgtcgagca ctgggagttc
     3181 gaggaggagg tgaaggcaat ggaagcggag ggcaagatcg ctcctaaggg caaggagatg
     3241 gtgctcggca tcaagatgtc ggcgctctcc cgtcgctcgt tcctctcggc cgcatccttc
     3301 gagcagacca cgaagatcct catcgacgcg tcactccgcg gttccgtgga cacgctccgc
     3361 ggtttgaagg agaacgtgat cctcggccgc ctcatcccgg ctggtaccgg cttcgcaggc
     3421 tccaagaagc gcgctgcgat tgaccagatg caggagaagc gtgcgcagga gatggcacgc
     3481 agggaggctg aacgcctcgc gatggaggag caggcaactg cgtaatctat caatgtgtga
     3541 aagcaaagcc cgcagcgatg cgggctttgc tcgtataaaa gaaaaccgcc cagagggacc
     3601 gggcggtctc ttggggcgga atcaatgaac cgacctgctt tcttgccgag gtgcagcgtc
     3661 gacgaacact gagatgtgtc tcggctgctg tctcggctcg ccctcacact gcatatgggt
     3721 gagcatggtg gcagtcgatt gaatgagtag cgattcgagg atctccggat cgagcccctc
     3781 gattggcacg tcaaacgtac gatagccgga cttctgccag acgtagatcc gcttatcgca
     3841 aatcggacag cgcgccaggg aagggaacgg gtgcaggttt ggattgcgca gatcgttgag
     3901 agatctgatg acgcttgcca cgatggaccc gacggtgatg gtgccgatga cctgcaagag
     3961 cagacgtacg gcttcggtga gtgtcatcgg acacctccag tgaattaaga tcagctaaaa
     4021 gtaatataca tatatttata gtaagtgtca attccacgga aaggtgcacg tcctcctgta
     4081 cacgttagaa tgtgacgcat ggacaccgtc gagcagatta aggtcaagct ctctatcgtg
     4141 gacgtggtgt ccccgtacgt gaagctcacg aaatccggga ggaactggaa gggcctttct
     4201 ccgttcaata aggaaaagac cccgtctttc tacgtaaatc ccgagcgcgg cacctactac
     4261 tgtttctctt caggacaagg tggagaccat ttcgagttca ttcaaagaat ggaaggcgtg
     4321 gacttcaagg gagcacttca catcctcgca gagaaggcag gggttgagat cgtatacaca
     4381 ggagggcagt ccagggaatc gaaggataag aaggatcgct tgtacgaggc gatggcgcga
     4441 gccgaaacct tgtatgcgga cttgcttaaa gatgggaaga gcgtcgcagg aagtgcatac
     4501 gcgtacgcta agtcccgcgg actctcggaa gagagtatcg cttcttggaa cttagggttc
     4561 gcccctgaag cctggcgcat gacgctcgag aagcttcagg aagcagggtt ccagaaccag
     4621 gaactcatcc aggcaggact cgtgaaggaa gcggacgaga agaagggaac gttctatgat
     4681 cgcttcagga accggctcat gttcccgatc cgggatagcg caggcaggac cgtggcgttc
     4741 acgggtcgcg cgcttgatcc gaacgatcag gcaaagtacc tgaattctcc tgagaccgat
     4801 ctctacaaga agtcagagat actctttggc atggaccggg cgaaggacgc gatacgccag
     4861 cgcggattcg cgcttttggt tgaagggcag atggatcttc tgatgctgca ccagataggc
     4921 ttcacgaata cgatcgcgct ttctggcact gcgctcacgc cgcagcatct ttcccttata
     4981 aagcgctacg cagacaacct catgttggcc ttggattcgg atagggcagg actgaacgcg
     5041 tccatgaaga acgcaatcgc agcgcttcgt gcgggtatgc gcgtgaaggc ggtgaagttg
     5101 cctgtgggta aggatcctgc agacctcgcg gttgaggatg cgaaagattt aacgaagcgt
     5161 atcgcggacg cgcagcctat cgtagagttc ttcctctcgg tgctcgccga aagcgaaggg
     5221 gatcagcacc gtcttattct cttggcagag aagacggtct taccgctcct tgccgctatt
     5281 gagagtccta tggaacggga gcatttcgtg ggtattgccg cacgcgcact cggctcaacg
     5341 cctgaagcga tccgtgctgg cttagtgcgc ctgccaaagg aggccgtgga agcaaagatg
     5401 gagactgtaa atctcccggg gaagtcagcg ccggttcgca ctccggtcca gaagctcggg
     5461 tcccggctcc atgcgatata catgcagtac cctgacagtc tcgttgccaa gcgagtagaa
     5521 actgagtata gtagggtcat cggcgcaccg ttcccagacg agccccctcc cgagaacgag
     5581 attttcgagg caggaataga ctatggcgag gctcctgggg aaacggacgc ggacgacctc
     5641 gttcgcgcat tcgagaaagc atatatcgcg gagcagctcc aggctgcgac cgcgcgcttg
     5701 cgcgtagctg agaccgcccg cgacgaagcc gccatcagag aggcagagca ggaatgccag
     5761 agactgacga gactccttgc atcgttttga tacatcctta ctaactcata tcggaaccca
     5821 ctatggctac caagaagaag gcggctaaga agcccgttaa gaaagcatcc gtgaagaaag
     5881 ccgttccaaa gaaagcggtg aagaagactg cgcctaagaa ggccgtgaag aagccggtca
     5941 agaaagccgc tccgaagaag gcggttaaaa aggctccggc caaaaagcct gcgaagaagg
     6001 aagttcttaa gaatgtcgtg cgtgtcgcag caaagggtgc gaaagcgaaa gccgaagcac
     6061 agaaggtaga caaagcagac cttctcatcc agaagggcaa ggagcgcggc tacataacgt
     6121 acaacgagat cctcaaggag tttccgcacg tggaggatga catcatgttc ctcgacgagc
     6181 tctatgagcg cttcactacc gctggcatcg acatcctcga gggcggtatg ctcgcggacg
     6241 atacggacca gtacctcgcc ggccgcaaca tcaagggtcg cgactcgggc gcatatgact
     6301 cgatccagat ctaccttcgc gagatcggcc agtacccgct tcttaccgca agcgaagagc
     6361 gcgaactcgc gcagcgtatt gagaagggag acgacgaggc gcgcaacatc ctcgcgcgct
     6421 cgaacctccg tctcgtggtg tctatcgcga agaagtacgt aggccgctcc ccggacctca
     6481 cgcttctcga tctcatccag gaagggaacc tcggcctctt caaggccgtc gataagttcg
     6541 atttcaaaaa gggattcaag ttctcaacgt acgcaacgtg gtggattcgt caggcaatca
     6601 cccgcgcgct cgccgaccag tcccgcacca tccgcattcc ggtgcacatg gtggagacca
     6661 tcgcgaagta caagcaggtc tcgcgccgtc ttgcgcaggc gcttggacgc gacgcacagc
     6721 cagaggagat cgcaattgag atgggtgtcg aggtagataa gatctaccag atc
//
LOCUS       DASWXU010000059        18864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U3693
            k141_970238, whole genome shotgun sequence.
ACCESSION   DASWXU010000059 DASWXU010000000
VERSION     DASWXU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959501
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:35:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 452
            CDSs (total)                      :: 429
            Genes (coding)                    :: 427
            CDSs (with protein)               :: 427
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18864
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970238"
                     /isolate="SMAG_U3693"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..3235
                     /locus_tag="VGE48_02150"
     CDS             29..3235
                     /locus_tag="VGE48_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-like domain-containing protein"
                     /protein_id="HEY1042199.1"
                     /translation="MAARGDIVFASGASGSYANAPERMRILASNGNVGIGSTTPSAKL
                     AIVDGGGSGDTTLALNNRFRFSGDGVMRWGITANQGIMSWDTGVAIIGSQTSNDLAFI
                     TNGSSISSERMRITTGGNVGIGTSTPAEKLVVTGDTRIVSGGSNTPSLYIQNTGTGGR
                     SWRFIASQTGDALNGGFGIYDSTTGAYRVALDSSGNVGIGTTSPQARLQINAASQVLG
                     SSVPTGALLITNTTGSGHGLEFGSDATNLGYIQSRNTSSQTYYQLLLNPSGGNVGIGT
                     TDPKSKLDVQGSPITGGTFTGNTPGIMVLAGDAYTNNDIVAMDFRPTYASLGINTLAR
                     IGAHMTDSGSYLKFGTSNNYSAGITNTALTIDPTGNVGIGDTSPSQRLVVSGAAVVTG
                     GVYGAETSAIDATIWAVSGDYTNWGIFYNEGTPDRIEFREGGNVNSAIGLDGSSYFNV
                     NSGNVGIGTASPNGTYKLDVQGGALNARIQSTSDAVLQLSGTDTYSGILFNDVNGNDP
                     FWYYGASDSFAIGGPGATSNKLNVYGNVAVGVNYNSVGAPTNGMIVEGNVGIGMNNPS
                     VALDVTGDIEYTGTITDVSDRRLKTNIQDINGALDILSAIRGTTYTMIDSGRSEVGFI
                     AQEVQAVLPNTAKVVDPRTGYLGVSYMDFIPYLVEGVNDLNVRTLSLAPAAQNQQAHS
                     LAVSADASVAGKLVVTATGAFGGNVSAQSFTTTGSVKATSFTVTTSDTLPNEVLTAGQ
                     ADLYKMARYAISEIKNLKERTDLLAVRIDEIETRLAALENQSATSTVAGAVGLTASTL
                     KSALESFGIFIENGIAQFNTLVFRQLAVAKDEAGDSSAGSVTILAGNTVTQVQNPYVL
                     PTSKVFVTFTSPVVGAWYISHKEAGSFRVTLAESQPSDVSFDYFILQTEGQLASPGAA
                     GPSQQNQVPTPAPQPFQPVFPQDGGTSTPSTSGDTTPPSISLNGSAAIEVGQGGSWND
                     QGATASDETDGDLTSQISVSGSVDTATPGLYTVTYSVSDAAGNQANVSRIVTVTAAPQ
                     TPTPAPTPAPTPEPAPAPTPEPAPAPEPAPTPAPAPSV"
     gene            3289..4299
                     /locus_tag="VGE48_02155"
     CDS             3289..4299
                     /locus_tag="VGE48_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEY1042200.1"
                     /translation="MAIRVAINGFGRIGRAFVRQTWNNPEIELVAINDLGSAENLAYL
                     LKFDTVYGRAPFPVLWKEGALSINGKEVRFLSEKDPALLPWKDMQVDVVVEATGFFTD
                     YEKANAHVTAGARRVVITGPVKEEHPLGATVLMGLDESKLGTCPITSNASCTTNSASP
                     VIAILDEAIGIEKAVLSTTHAYTASQSIVDGPSKKDLKEGRAAAQNIVPTSTGAAVAV
                     TKAYPSLSGKFDGISLRVPVPAGSIADITFIAKRPTTAEEVNDILRKAAELPRWQGIF
                     RASDEEYVSSDIVGEHAASIADLGMTRVVDGNLVKVLAWYDNEAGYTATLVQHVLKAG
                     SM"
     gene            4302..4559
                     /locus_tag="VGE48_02160"
     CDS             4302..4559
                     /locus_tag="VGE48_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042201.1"
                     /translation="MGVTFEEETQFQTTRSASSVRGLTAWVIQKKLASDEKSANMLLL
                     GVVGACILISIIVFTVLMGDSRQLTDAERLRQERSTPLPRR"
     gene            4563..5024
                     /locus_tag="VGE48_02165"
     CDS             4563..5024
                     /locus_tag="VGE48_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1042202.1"
                     /translation="MTSRGFTLIELLVVIAIIGVLSSVVLASLNSARQRARDAAVKTG
                     VRELSTLMQLEYNDTGSFANLQSGWDYNATDCNNSFAGSYATNARQICTSVVSNGGAM
                     YTGNNTSLSTHFSIMGLLPGAGRYYCRGSSGGTSDTETGSNWLSAGCYANP"
     gene            5084..5473
                     /locus_tag="VGE48_02170"
     CDS             5084..5473
                     /locus_tag="VGE48_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042203.1"
                     /translation="MELDAQTAPTHTRRIVLIILASIGILALVGAAYLFLERPWGPKK
                     LDLGITSITSYVCPDDSHYVVIKKENEISVSGQAFSRIDDVRFGNGGNVEFMIEGTDL
                     VVRNTETDENTRCTEGLPEGGMPPLVL"
     gene            5481..6977
                     /locus_tag="VGE48_02175"
     CDS             5481..6977
                     /locus_tag="VGE48_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEY1042204.1"
                     /translation="MNTYIFFRTAVLQALAVFVLFFALATSVYAGCGIVCTEPRSDGT
                     PERGDFYTNGCLSGTGTLDTSTCDSGGGSDGGGGGGGGGGGGGSFADLTASDVTPVMA
                     TSGEAQTYYATVYNGATNVGNTTTLLQRTENPGDISTPVVSMIATASMNAYSSRTVSA
                     SYTHTGSSFYMRACADSGNQVAESNESNNCGPWTLITVMQPPPQQQADLIAGSVSVSN
                     AVQGAAATLSASASNIGAAASESFPILFQVSETGARINSAYLGGLAAGGSAGGTASYK
                     FPDFGVYNVRACANFDTAWNPIAAESSTANNCGPWAEVTVSPVVTPALSCSVSASSVA
                     VGGSVTYNANPSGGASGPYTWTASDGGSFGTAASVTRSFSAPGTYAMNVDTSSTAVSY
                     CPNVSVVSTACSGAGTNLTITATPSRVRAGQASTISWTASGVNGEGASCTVSGPGISW
                     TSGVTAAPQCSAGGSSNVTIATQSTYTITCAGQSESVVVNVIPQIIEF"
     gene            6981..7472
                     /locus_tag="VGE48_02180"
     CDS             6981..7472
                     /locus_tag="VGE48_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEY1042205.1"
                     /translation="MPKVYIASDHTGFLLKGELVTYLTETLLYEVEDLGAHELNPEDD
                     YPDLITPCAKKVASTPDTLGIVIGGSGQGEAVAANRIPGVRAALFYGEARAVGAIDAE
                     GAPALDGYDIVRLARVHNDANVLSLAARFLDTDTAKEAVRIFVSTAFSGVERHTRRIQ
                     KLG"
     gene            7494..8216
                     /locus_tag="VGE48_02185"
     CDS             7494..8216
                     /locus_tag="VGE48_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042206.1"
                     /translation="MGIIVPAILPVSREDLEQKLDILDGVCEEVQVDIVDGVYASPKT
                     WPYTRDASEPSRMLAEGEFLPKAGRFRIEIDLMSGDPESVAGTWIGLGAARLTIHAQS
                     TRYLPRFLASTRSLYGHDKEFESDLLSLGLALGAETDLSLIEPYIDKIDYVQFMGIRT
                     IGRQGEPFDMRTVAKVRAFRKKYPKTPVQVDGGITLKNAAELLDAGVSRLIVGSAIWR
                     AENPLDAYRALNTLTEQHGLYA"
     gene            8209..8736
                     /locus_tag="VGE48_02190"
     CDS             8209..8736
                     /locus_tag="VGE48_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY1042207.1"
                     /translation="MHNHRGFTLIELLVVIAIIGVLSSVVLASLNQARIKARDTSRIS
                     QLAEIRKALELYYLDNGYYPASNCGWDCNGYRYSYDSSWNTLASDLAPYITLPRDPVN
                     TGCTPWSANCFSYAYGNVGRSTYAPQYDLTAQLEDTNSPYRCAARGYRFYFNNQGWCS
                     ASGGSYSDQIYEASP"
     gene            8742..8984
                     /locus_tag="VGE48_02195"
     CDS             8742..8984
                     /locus_tag="VGE48_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042208.1"
                     /translation="MSNVVFNEEQTPTRQAVSQDPLFVRLLIKYQIVKTRKGAEYVLI
                     AATIVACLVAIIVFPKGRPSPGVNIPVAGPMDTRTR"
     gene            9009..9866
                     /locus_tag="VGE48_02200"
     CDS             9009..9866
                     /locus_tag="VGE48_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY1042209.1"
                     /translation="MALTDTDVQMLEEKAEAIRETILDMLVQAGSGHTAGPLGMSDLF
                     SALYFRVLKHDPKNPDWEERDYLVLSNGHTAPVRYSAMAHAGYFPVEECLTLRKFGSR
                     LQGHPERTKLPGLETTSGPLGSGLSQAAGMALALSMNRKKNRVYCMMSDGEQQEGNTW
                     EAVLFASKYRLGNLTAIMDRNFIQIDGMTEDVMPLDSLKAKYEAFGWEVFEIDGHNME
                     AIVDACEEAKAIVEKPTLIIARTIPGKGVPEIEYDYRWHGKPPTKEEAARFMKELRNG
                     AGRVPHEYA"
     gene            9859..10410
                     /locus_tag="VGE48_02205"
     CDS             9859..10410
                     /locus_tag="VGE48_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY1042210.1"
                     /translation="MRNRGFTLIELLVVIAIIGILSSVVLASLNTARERAKIANAVAQ
                     LRELRNVVAAYYADTGRYPADCGLTCTQSTDPYLNALGVSGWAGPYFSGGVWNLRHPW
                     NGHFSIASADVTGDGTPELYFFLDEDAPGTDSNNNQGVISTESLQRIDQIIDDGNLAT
                     GKARGDGNGFTSAVGELVFLPEL"
     gene            10433..11422
                     /locus_tag="VGE48_02210"
     CDS             10433..11422
                     /locus_tag="VGE48_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1042211.1"
                     /translation="MINPAARLSDALFSKDIERKPTRDGFGHGTVAAGKKDENVVVLC
                     ADLKESTRAEWFEKEFPDRFIEVGVAEQNLATAAAGMAAIGKRPFIASYAAFSPGRNY
                     EQIRTTIALNEQPVVVCGMHAGVSVGPDGATHQMLEDIGLMRMLPGMAVIVPGDAVEA
                     QKAVEYAAAASHPTYIRFGRSPVPTFTTPETPFSYGKAISVWESEKPKAALMATGALT
                     YEALVAAKALAADGIEVTVLHVPFVKPLDEEAVLAAAKRAGRVITIEEHQAAGGFGSA
                     VAEFLSSVYPVPVTRLGIQDQFGQSGEPQELLSHYGLTAPEITAAVRKSLGVY"
     gene            11428..11592
                     /locus_tag="VGE48_02215"
     CDS             11428..11592
                     /locus_tag="VGE48_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042212.1"
                     /translation="MKGKKLSGYAVAFIFFMLGVAATLLILTAANRAESMHEDYDSIN
                     PADLNADFGH"
     gene            complement(11619..12611)
                     /locus_tag="VGE48_02220"
     CDS             complement(11619..12611)
                     /locus_tag="VGE48_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY1042213.1"
                     /translation="MSIDFLAIGDTVIDDFIRLKDARSHCNIDDGSCEICMRFGDKIP
                     FESSTKVVGVGNSANAAVSAARLGLSSSLITNVGKDAYGNEVLESLSSKGVDTTYVTL
                     QEDKKTNYHYVLWFESERTILVKHETYEYAFPKDLPEIKTLYLSSLGEVPDTYYDAIA
                     DFAESRPHMRLVFQPGTFQMAMGTERLKRIYARSDLFFCNKEEAARILKREGEQDIKE
                     LLSGMHGLGLKTVVITDGREGAHASDGTNVYFIPMYPDTREPYERTGAGDSFASTVSV
                     ALTLGKPLEEALLWGPINSMSVVQAVGAQEGLLSREALEAHLEGAPSTYALTKL"
     gene            complement(12634..13080)
                     /locus_tag="VGE48_02225"
     CDS             complement(12634..13080)
                     /locus_tag="VGE48_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1042214.1"
                     /translation="MKSRGFTLIELLVVIAIIGVLSSVVLASLNTARIRAVEAQKKAE
                     LRQIALALNNYYLATGLSPANSPAPSNWNVADTALSSALEPTYMASIPNFSDSSYPYY
                     YYDYGSYFLVASYIGDTYGPGTQGWHCSDAAGGTPGSRYWCIEIPK"
     gene            complement(13077..13331)
                     /locus_tag="VGE48_02230"
     CDS             complement(13077..13331)
                     /locus_tag="VGE48_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042215.1"
                     /translation="MNVTFEEEQTLAPAASRTTRVSGLYALVIRWGLAKDEREASIAL
                     IVIAALAGLLAVTVWLVAGGFEPEGTTHKELIQMQRREGV"
     gene            13410..14408
                     /locus_tag="VGE48_02235"
     CDS             13410..14408
                     /locus_tag="VGE48_02235"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY1042216.1"
                     /translation="MTDLSDIARGMFAPGKGILAADESNESADEKRLKPFGIEPGPEM
                     RRKFRDLLLATPGIEQYLTGVILYKETLEQESLQGIPFPKLLLDKGIAPGIKVDEGLD
                     PLEGSPDEMITKGLLGLPERLIEYRERHQASFTKWRAAIRISGDRLPTKKALLENAKR
                     LASYACEVQRAGMVPMLEPEVLLEGSHSRLRARKVLEESMTILFDTLNEQCADLSGVI
                     VKTSMALSGSETGRMDTPEEVAEDTLGALMATVPAGVGGIVFLSGGQTPDQATENLRA
                     ISRLAKKLKAPWPLTFSYARAFQEEALHIWKGKDENVEAAREAFLARLKKASEALV"
     gene            14418..14651
                     /locus_tag="VGE48_02240"
     CDS             14418..14651
                     /locus_tag="VGE48_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1042217.1"
                     /translation="MDVQFDETSYAPRTTQAARVSTMTRLVIKAGLAKDEKEANKVFL
                     WIIGLCLLIPLGLWLVPALMPDPEIQPIVLPRQ"
     gene            14648..15091
                     /locus_tag="VGE48_02245"
     CDS             14648..15091
                     /locus_tag="VGE48_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1042218.1"
                     /translation="MNHKRGFTLIELLVVIAIIGVLSSVVLASLNTARTKSRDAARVA
                     TMKQIQNALALYHLDRGYYPPMTENECGGTEGYTTSNNNFMQGLVTTGYLPSYPIDPG
                     NLSCGMQYYPSGNNAYYLFYRLEAGPTAPATGCNQPPTWYCIAGG"
     gene            15142..15768
                     /locus_tag="VGE48_02250"
     CDS             15142..15768
                     /locus_tag="VGE48_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY1042219.1"
                     /translation="MQHNLTVSPRDIVGKSSGKLAGEGLIPAVVYGPKQEALSISVPL
                     LPFEAVLRHGGESALIELSGLPKPMQVLVHDLDRDPVTHVPRHVDFYAVEKGAKVQLM
                     VPLSFVGESMALKQGANLVKVLHEVEVECDPSKIPTEIEVDTAALAAEGDQIHVSDIK
                     APSGVAILTPGEEVAAIAQAVEEESEESATLDMDAIEVEKKGKEEEGE"
     gene            15852..17183
                     /locus_tag="VGE48_02255"
     CDS             15852..17183
                     /locus_tag="VGE48_02255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY1042220.1"
                     /translation="MKRIVVVLSVFALLIAGPAAILAQELTASERAELERQLADLERE
                     MQKTQATIDNLSAQGASLQRDIAILDGEIKRARLKVQTAEAEIRALAAGIVVHTRTIG
                     TLSEKMIREQDSLAQILRKTDAIDDYTLVEAVLSGAEISDFFGDLDTFASIKQAMRTS
                     FEELRATKAETQEERAGLESQKTDQEILRSALVLAQREVEAKEAEKKKLLAETKGQES
                     LYRGLKSSQEQTAAQIRAKLFPLRDTSGIQFGDAVRYAQAASARTGVRPALILAILSQ
                     ESDLGKNVGQCLITDLATGDGKGKNTGTPFPGTMKAPRDTVPFERLMKAAGRDPLNTP
                     ISCPLPGGYGGAMGPTQFIPSTWEMYEGRIKAALGVSAADPWNASHAVMATGLYLADV
                     GAAGGTYLAEHTAAARYYAGGNWATSGQGYGNSVMSKAAAFQKDIDFLEAN"
     gene            17242..>18864
                     /locus_tag="VGE48_02260"
     CDS             17242..>18864
                     /locus_tag="VGE48_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEY1042221.1"
                     /translation="MYKTLFLFLVVGMGVAAFFLAPDTANAAACNFTNTNINIAWSPS
                     DPYDQQGGSGSYAYTPGTVVARSYGDLSLNNQTNRNWVTTVTVTNNGVTRTLTNSSCT
                     WADIDNPCYSQTGAQDVGGAMFNLYFEVQHYIKAELTCTPSPDVTPDLVGQVGGARTG
                     QVQQPMTLYGAIANQGGAAGAFENIIQVCDQSCFTLNQILSATTLSSLGAGSSAAISA
                     NYTPPSGMGTSDFMYRVCANTRVVTSKGSSTWSNPATESNYANNCSGWQSLTIQQEPV
                     PPTANLTLTPTLIAYGGASILQWGSTNATSCTGTNFSTGGATSGSLSVTATQDTTYTV
                     TCTGPAGSASDSELLSVGAQPQPDLVAGSVTAGSPQAGVSATLSANATNVGPGTSGAF
                     PILFQVSETGALAQSGYLAALSSGASGSGSASYTFPSAGTYQVRACANYNTSWTAITT
                     ESNYANNCGPWTNVTVASAPQPPALSCSVSQASVTPGGSVTYSANPSGGASGPYTWTA
                     ADGGSYGTGSTASRTFSTPGTYAMNVDTSSTAV"
BASE COUNT         4365 a         5179 c         5316 g         4004 t
ORIGIN      
        1 caactcgtca acgaatataa acctcgccat ggcagcgcgt ggcgacattg tgtttgcttc
       61 aggtgcttct ggttcatatg ccaacgctcc ggagcgtatg cgtattttgg cttcaaacgg
      121 gaacgtcggt ataggttcaa ccacgccatc agcaaagctt gcaattgtcg atggaggtgg
      181 cagtggcgat accacacttg ccctcaataa ccgtttcaga ttctctggag atggcgtaat
      241 gcgttgggga attactgcaa atcaaggaat catgagttgg gatacgggtg ttgcgattat
      301 tggatcgcaa acttcaaatg acctggcgtt cattacgaac ggctcaagca tttcaagtga
      361 acgtatgcgc ataacgacag gtggcaacgt cggtatcggc acctccacac cagcagaaaa
      421 gcttgtagtt actggcgaca cccgcatcgt aagtggagga tcaaatacac catcgctcta
      481 catacagaat actggaacag gaggcaggag ctggcgtttc attgcgagcc agacgggtga
      541 cgcattgaac ggaggatttg gaatctatga ttccacaacg ggagcctatc gcgtcgctct
      601 cgattcatca ggcaacgtcg gtatcggcac gacgtcacct caggctaggt tgcaaattaa
      661 cgcagcgagc caggtccttg ggtcctctgt tccaaccggt gctcttctta taacgaatac
      721 aacgggtagt ggacacggac ttgagtttgg atcggacgca accaacctgg gttacataca
      781 atcaagaaac acctccagcc aaacgtatta ccaattactc cttaacccga gtggtggaaa
      841 tgttggtatc ggcactacgg atccaaaaag taagctcgat gtacagggta gtccaattac
      901 gggcggtaca tttactggca acacgcctgg catcatggtc ttggcgggcg atgcgtatac
      961 caacaacgat atagtcgcca tggatttccg acctacctat gcatcactcg gcatcaacac
     1021 gctcgcgcgc ataggtgcgc acatgaccga ctcaggtagc tatctgaagt ttggtacgag
     1081 caataattat agcgctggta ttactaatac tgcattgacc atcgatccga cgggtaatgt
     1141 tggtatcgga gatacatcgc catctcagag gcttgtggta agtggagcgg cagtcgttac
     1201 gggcggcgtg tacggagccg aaacctcagc tattgatgcg accatctggg cagtctcagg
     1261 tgactatacg aactggggta tcttctataa cgaaggaact ccagaccgaa ttgaattccg
     1321 cgaaggcgga aacgtgaatt cggctatcgg tcttgatgga tcgtcatact ttaacgttaa
     1381 ctcaggtaac gttggtatcg gaactgcgtc tcctaacggt acatataagt tggatgttca
     1441 gggaggagca ctgaacgcga ggattcaaag cacctcggac gccgtacttc agctttcggg
     1501 taccgatacc tattcgggta tcttgttcaa cgatgtaaat ggaaatgatc cattttggta
     1561 ctacggtgca agcgattcat tcgctattgg tgggccaggc gcaaccagca acaagctcaa
     1621 tgtgtacggc aacgtggccg tgggtgttaa ctacaacagt gttggcgcac caacaaacgg
     1681 catgattgtg gagggtaacg tcggtatcgg aatgaacaat ccatcggttg cactcgatgt
     1741 tacgggtgat attgagtaca cgggcactat taccgacgtc tcggatcgtc gactcaagac
     1801 gaacatccag gatatcaacg gcgcactcga cattctctcg gcgatccgcg gtaccaccta
     1861 cactatgatt gatagtggtc gatccgaagt gggcttcatc gctcaggagg tgcaggcagt
     1921 actaccgaac accgcgaagg tggtagatcc tagaaccggc tacctcggtg tgtcttacat
     1981 ggacttcatt ccatatcttg tggaaggtgt gaatgacctc aatgtccgca cactttccct
     2041 ggctccagct gcgcagaacc agcaagcaca ttcgctggca gtctctgccg acgcttcggt
     2101 tgccggaaag ctcgtggtta ctgcaaccgg cgcgttcggg ggtaatgtat cagcacagag
     2161 ctttactacc acaggctcgg ttaaggcaac ttccttcact gtaactacga gcgacacgct
     2221 tccaaacgaa gtgcttactg cgggacaggc agacctctac aagatggcgc gctacgcgat
     2281 ctcagagatc aagaacctta aggagcgcac tgatcttctt gcggttcgta ttgatgaaat
     2341 tgagacccgt ctcgcagcgc ttgaaaacca gagtgcaacc tcaaccgtag ctggcgccgt
     2401 tggtctcacc gcaagcactc ttaagagcgc acttgagtcc ttcggcatct tcatcgagaa
     2461 cggcatcgcg cagttcaata cgctcgtgtt ccgtcagctt gcagttgcta aggacgaagc
     2521 aggcgactct tctgcaggta gcgtcacgat cctcgcgggc aacaccgtta cccaggtaca
     2581 gaacccgtac gtgctcccaa cctcgaaggt attcgtaacc ttcacgagtc cggtagtagg
     2641 tgcgtggtac atcagtcaca aggaagcagg cagcttccgc gtgacgctcg cagaatcgca
     2701 gccgtcagac gtctccttcg actacttcat ccttcagacc gaaggtcagc ttgcaagccc
     2761 gggcgctgca ggaccgagcc agcagaacca ggttccgacg ccagcaccgc agccgttcca
     2821 gccggtattc ccgcaggatg gaggaacctc gacaccatca acttcaggag acactacgcc
     2881 gccatccatc tcccttaacg gaagcgccgc tatcgaggta gggcagggag gctcttggaa
     2941 cgatcagggc gcgactgcgt cagatgagac agatggagat ttgacgagtc agatttcggt
     3001 atcaggctcc gttgatacgg caactccagg tctctacacc gttacctact ctgtatcaga
     3061 cgcggcaggc aatcaggcga acgtatcgcg catcgtgacg gttaccgcag caccacagac
     3121 tccaactccg gctccaactc cggcaccaac tccggaaccg gcaccagccc cgactccaga
     3181 gccagctccg gcacctgaac ctgctccaac tcctgctcca gcaccttccg tatagcggag
     3241 ggtagggatg gtagacttta tccatattca ggtagcgaac gcacatctat ggccattcgg
     3301 gtagcgatta acggtttcgg gcgtatcggc agggcattcg tccgccagac atggaataac
     3361 cctgagatag agctcgtggc cataaatgat cttggctctg cagagaacct cgcatatctt
     3421 cttaagttcg acacggtata cggccgcgcg ccgtttccag tcctgtggaa ggaaggagca
     3481 ctttccataa acggaaagga ggtccgtttt ctttcggaga aggacccagc actccttccg
     3541 tggaaggaca tgcaggtgga cgtggtcgta gaagcaaccg gcttctttac ggactacgag
     3601 aaggcgaacg cacatgtaac ggcaggtgcg cgtcgcgtcg ttatcacggg accggtaaag
     3661 gaggagcatc cgctcggcgc cacggtcctc atgggacttg atgagtcgaa gctcggaacc
     3721 tgcccgataa caagcaacgc gtcttgcacc actaactctg cgtctccggt gatcgcgatc
     3781 ctcgacgagg cgatcggtat cgagaaggcc gtgctctcga ccacgcacgc ctataccgcg
     3841 agccagtcca tcgtggatgg tccgtcgaag aaggacctta aagagggaag ggcggcagcg
     3901 cagaacatcg taccaacctc aaccggtgct gcagttgccg taacgaaggc gtacccgtcg
     3961 ctttccggga agttcgacgg catctcgctt cgcgttccgg tgccagccgg ctccatcgcg
     4021 gacatcacct tcatcgcgaa gcgtccgacc accgcagaag aagtgaacga catcctgcgg
     4081 aaggccgctg agcttccgcg ttggcaaggt atcttcagag cgtcggacga ggaatacgta
     4141 tcaagcgaca tcgtgggcga gcacgcagca tcgatcgcag atcttggtat gacgcgtgtc
     4201 gtcgacggga atctcgtgaa ggtgcttgcg tggtacgaca acgaggccgg gtataccgct
     4261 acactcgtgc agcacgttct caaggcgggg agcatgtagc tatgggagta acctttgaag
     4321 aagaaacgca attccagacc actcgttccg cgtcgagcgt aagaggtctc accgcgtggg
     4381 taattcaaaa gaaactcgct tcagacgaaa agtccgcgaa catgctcctg cttggtgttg
     4441 ttggagcatg tattctcatt agtataatcg tgtttacggt gcttatggga gattcgcgtc
     4501 agctcaccga cgctgaacgc ttgcgacagg agcgatcaac gccactccct cgacgctaat
     4561 gtatgacttc tcgcggattc acactcattg agcttcttgt ggtgatcgcg atcatcgggg
     4621 ttctctcctc cgtcgtgctt gcgtcactta atagcgctcg tcagagagct cgcgatgcag
     4681 cggtgaagac gggagtgcgc gagctctcga cactgatgca gcttgagtac aacgatacgg
     4741 gaagctttgc gaacctgcag tcaggctggg actacaacgc tacagattgt aataacagtt
     4801 ttgccggctc gtatgcgaca aacgctcgac agatatgtac gagcgtagtc tcaaacggcg
     4861 gggcaatgta tacagggaat aacacgagtc tgagcacgca cttttctatc atgggtctcc
     4921 ttcccggtgc cgggaggtac tattgccggg gtagtagcgg tggcacgtct gataccgaaa
     4981 caggaagcaa ctggctttca gctggttgtt acgctaatcc ttagtccttt cgttcacata
     5041 gacaggcgac aattcagact ttgtgcgtgg tatcgtttta tatatggaac tcgacgcaca
     5101 gactgcaccg acgcacacac gaagaatcgt actcattatt cttgcaagta tcggtatact
     5161 cgccctcgtt ggagccgcat atctctttct tgagcgaccg tggggaccga agaagcttga
     5221 tctcggaatc acgtctatta ccagctacgt ctgccctgac gattcgcatt acgtcgtgat
     5281 taaaaaggag aacgagatat ccgtctcggg tcaggcattc tcaagaatcg atgacgtacg
     5341 cttcgggaac ggagggaacg tcgaattcat gattgaaggc acggatctcg tggtgcgtaa
     5401 taccgaaacg gacgagaaca ctcgctgcac cgaaggtctt cctgaaggcg gcatgcctcc
     5461 actagtcctc taatagtcat atgaacacct acatcttctt ccgaaccgca gtacttcagg
     5521 cgcttgctgt gtttgtgctg ttctttgcgc ttgctaccag tgtctacgca gggtgtggca
     5581 tagtctgtac ggagccacgt agtgacggga cgccagagcg cggagatttc tacacaaacg
     5641 gctgtctaag cggcaccggc acccttgata cgagcacctg cgatagcggt ggtggaagtg
     5701 acgggggtgg aggaggagga ggcggaggcg gaggaggtgg cagctttgct gacctcaccg
     5761 caagcgacgt cacaccggtt atggctacga gcggagaggc gcagacctac tacgcgaccg
     5821 tgtataacgg cgccacgaac gtagggaaca ccacgactct tctgcagcgc actgagaacc
     5881 caggggacat aagtacgccg gtagtgagta tgatcgcaac cgcaagcatg aacgcgtatt
     5941 cgtcacgaac ggtctctgcg tcctacaccc ataccggaag ctcgttctac atgcgtgcct
     6001 gtgcggattc ggggaatcaa gtcgcagaga gtaatgaaag caacaactgc ggcccgtgga
     6061 ctcttatcac cgtcatgcag ccacccccac agcagcaggc agatctcatt gcaggaagcg
     6121 taagtgtctc aaacgcagta cagggtgccg cagcgacact gagcgcaagt gcatcaaata
     6181 tcggcgcggc agcttcagag agcttcccaa tcctgttcca ggtatccgag accggagcgc
     6241 gtatcaacag cgcgtatctt ggagggctcg cagcaggcgg ttctgcagga ggcaccgcct
     6301 catacaagtt cccggatttc ggagtttaca acgtgcgtgc gtgtgcaaac ttcgacaccg
     6361 cgtggaatcc tatcgcggcc gaatcaagca ccgcgaacaa ctgcggtccg tgggctgagg
     6421 ttacggtttc gcctgtcgtt acgcctgcgc tctcatgttc ggtgtcagcg tcatcagttg
     6481 cggttggcgg ctcagttacc tacaacgcga atccgtcagg aggtgcatca ggtccctaca
     6541 cctggacggc gtctgatggc ggcagcttcg gcaccgctgc ttcggtaacg cgcagcttca
     6601 gcgcgcctgg tacctacgcg atgaatgtgg acacgtcttc aaccgcagta tcgtactgtc
     6661 cgaacgtgag cgtggtctca acggcatgtt caggagcagg taccaacctt acgattaccg
     6721 caaccccatc acgtgtgcgc gcaggtcagg cctcgaccat ctcctggacc gcgtccgggg
     6781 tgaacggtga aggtgcaagc tgtaccgtat cgggtcctgg aatctcctgg acctcaggcg
     6841 taacggcggc gccgcagtgt tccgcaggcg gctcctcaaa cgttactatc gcgacccaga
     6901 gcacctacac catcacgtgc gccgggcagt ccgagagcgt tgtagtgaac gtgattccgc
     6961 agatcattga gttctaattc atgcctaagg tatatattgc gagcgaccac acgggtttcc
     7021 ttctcaaggg agaactcgta acctatctca ccgagacgct cctgtacgag gtagaggatc
     7081 ttggtgcgca tgaactcaat cctgaggatg attacccaga cctcatcaca ccgtgcgcaa
     7141 agaaggttgc aagcacgcct gatacactcg gcatcgttat tggaggatca ggacagggag
     7201 aggcggtagc agcaaaccga atcccaggag tgcgtgcggc gctcttctat ggagaagcga
     7261 gagctgtagg cgctatagac gcggaaggcg cgcctgcact tgatggctac gatatcgtgc
     7321 gccttgcgcg cgtacataac gacgcgaacg tactttcact cgcggcacgt ttcctggata
     7381 cggataccgc aaaggaagcg gtacgcatat tcgtttcaac tgcgttttca ggcgtcgagc
     7441 ggcacacccg gcgtatacaa aagctcgggt aggagcgcgc tatactaagg tgcatgggaa
     7501 tcatcgtccc ggccatactt ccggtatcgc gcgaggacct cgagcagaag ctcgatattc
     7561 ttgatggtgt ctgcgaagag gtgcaggtag atattgtaga cggcgtctat gccagtccca
     7621 agacctggcc atatacgcgt gatgcttctg aaccgtcacg catgcttgca gaaggggagt
     7681 tcctcccgaa agccgggagg ttccgtatag agatcgatct catgagcgga gatccggaat
     7741 ccgtggcggg aacctggatc gggcttggtg cggcgcgcct tacgatccat gcgcagagca
     7801 cacggtacct accgcgtttc cttgcaagca cccgctctct gtacgggcat gataaggagt
     7861 tcgagtccga cctgctttcg ctcggtcttg cgcttggcgc ggagaccgac ctctcactca
     7921 tcgagccata tatcgacaaa attgattacg tgcagttcat gggtattcgt accataggca
     7981 ggcagggcga gccgtttgat atgcgtaccg ttgcaaaggt gcgggcattc cggaagaagt
     8041 atccgaagac accggtgcag gtggatggag gtataaccct caagaatgcc gcagagctcc
     8101 tcgatgcagg ggtctcgcgt ttgatagtag gttctgcgat ctggcgcgcg gagaatccgc
     8161 ttgatgcgta tcgcgcgctt aacaccctta cggaacagca tgggctttat gcataaccat
     8221 cggggattta ccctcatcga gcttctcgtg gtgatcgcca tcatcggcgt gctctcgtcg
     8281 gtagtccttg caagtctcaa tcaggcgcgc atcaaagcgc gcgatacgtc ccgcatatcg
     8341 cagctcgctg agatacggaa agcgctcgag ctctattacc tcgacaatgg ctactatccg
     8401 gcatcgaact gcggctggga ctgcaacgga taccgctata gctatgacag ctcgtggaat
     8461 acgctcgcga gcgacctcgc tccgtacata accctgcctc gcgatcccgt gaataccgga
     8521 tgcacgccgt ggagcgcgaa ctgcttctcg tacgcgtatg ggaatgtagg gcgtagtaca
     8581 tacgcgccgc aatacgatct tacggctcag cttgaggaca ctaatagccc gtaccgctgc
     8641 gctgcccgtg gctatcggtt ctatttcaat aatcaaggct ggtgcagcgc gtccggcggg
     8701 tcctattcag atcaaattta cgaagcatcg ccataatctg tatgtccaac gtcgtattca
     8761 acgaggaaca aactcccacg agacaggcag taagccagga cccgctcttt gtgcggcttc
     8821 tgatcaaata ccagatagtg aagactcgta agggagcaga gtacgtactt attgcagcga
     8881 ctatcgtcgc atgccttgtc gccatcatcg tgttcccgaa gggaagaccg agtccaggag
     8941 tgaatatccc ggtcgccggt cccatggaca cacgtacacg ctagaagcgc gcggtatact
     9001 gaagagtaat ggcgctaact gacactgacg tacagatgct cgaagagaag gcggaagcta
     9061 tccgcgagac gatactcgac atgctggttc aggcgggaag cgggcatacc gcagggccgc
     9121 ttggcatgag tgatcttttt tctgcgctct atttcagagt gctcaagcac gatccgaaaa
     9181 atccagattg ggaagaacga gactatctcg ttctcagcaa cgggcacact gcaccggtgc
     9241 gctactctgc gatggcgcac gcgggctatt tcccggtaga ggaatgcctc acattgcgca
     9301 agttcggctc acgcctccag gggcacccgg agcgcacgaa gcttccgggg cttgagacta
     9361 catcaggccc ccttggaagc ggcctttcgc aggcggctgg tatggcgctc gcgctttcca
     9421 tgaatcgtaa gaagaatcgt gtgtactgca tgatgtcaga cggcgaacag caggaaggaa
     9481 acacatggga agccgtgctg tttgcgtcca agtaccggct cgggaacctt acggccatca
     9541 tggatcgcaa ctttatccag atagacggca tgactgaaga cgtgatgccg cttgattcgt
     9601 taaaggcgaa gtacgaggcg ttcggctggg aagtgtttga gattgatggg cacaatatgg
     9661 aagccatcgt ggatgcgtgt gaagaagcga aagccattgt agagaaacct accctcatca
     9721 ttgcgcgcac gattccaggc aagggcgtgc cagagatcga atacgattat cgctggcacg
     9781 ggaagccgcc gacgaaagag gaagccgctc ggttcatgaa agagctccga aacggcgccg
     9841 gtcgcgtgcc gcacgagtat gcgtaaccga ggattcacac tcatcgaact attggtagtg
     9901 atcgccatca tcgggatact ctcctcggtg gtgctcgcat cccttaacac cgcccgcgag
     9961 cgcgccaaga tcgcaaacgc ggtagcgcag ctgcgcgagc tccggaacgt ggtcgccgcg
    10021 tactacgctg acaccggcag gtatccagcg gactgcggac tcacctgcac gcagagcact
    10081 gacccgtatc tgaacgcgct tggtgtctct ggctgggcag gaccgtattt ctcaggaggt
    10141 gtttggaatc tgaggcatcc ttggaatgga cacttctcaa tcgcaagtgc tgacgttacg
    10201 ggagacggta ccccggagct ctatttcttc cttgacgagg atgcaccagg aacagactcg
    10261 aacaataacc agggtgtcat ttcgaccgag tccctgcagc gcattgatca gatcattgat
    10321 gacggaaacc ttgccacagg aaaagcgcgc ggggatggca atggttttac atcagcggta
    10381 ggcgaacttg tatttttacc ggagctttaa acaaacgcag tatacttttt ctatgattaa
    10441 cccagccgcc cgtctttcag acgcactgtt ctcaaaggac atagagcgca agcctacgcg
    10501 tgacggcttc ggacacggca cggtagcagc ggggaagaag gacgagaacg ttgtggttct
    10561 ctgtgccgat ctcaaggaat cgacgcgcgc agagtggttc gagaaggagt tcccggatcg
    10621 tttcatcgag gtaggtgtcg ctgaacagaa cctcgcgacc gcagctgctg gcatggcagc
    10681 gatcgggaag cgtccgttca ttgcaagcta cgccgcgttc agtcctggta gaaactacga
    10741 gcagatccgc accaccatcg cgcttaacga gcagccggtc gtagtgtgcg gtatgcatgc
    10801 gggtgtatcg gtgggccctg acggagcaac ccaccaaatg ctcgaagaca tagggcttat
    10861 gcgcatgctt ccgggaatgg ccgttatcgt gccgggggac gcggtagagg cgcagaaggc
    10921 ggtcgagtat gccgctgctg cttcgcaccc aacctacatc cgctttggtc ggtctccggt
    10981 cccaaccttc accactccag aaactccgtt ctcatatggc aaggcgattt cagtatggga
    11041 gagcgagaaa ccaaaggcag cgctcatggc aactggcgca cttacctacg aagcgctcgt
    11101 cgctgccaaa gcactcgcgg ctgacggcat cgaggtaacc gtgctccatg ttccgttcgt
    11161 taagccgctc gatgaggaag cggtgcttgc cgcagcaaag cgcgccggcc gcgtcatcac
    11221 aatcgaggaa catcaggcgg ctggcgggtt cgggagcgcc gtagcggaat tcctttcaag
    11281 cgtatatccg gtaccggtca cgcgtcttgg tatacaggat cagttcggac agtccggtga
    11341 accgcaggaa ctcctttcgc actatggcct cacggctccg gagataaccg ctgcagttcg
    11401 caaatcgctc ggggtatact aatgcgcatg aaaggtaaga agctctcagg atacgccgtc
    11461 gcattcatct tcttcatgct cggggtagca gcaacattgc tcatccttac ggcagcgaac
    11521 cgcgcagaat cgatgcatga ggattatgac tctataaacc ccgctgatct gaatgcggat
    11581 tttggacact agcaaaatgg ccccgagggg ccgttttgct aaagcttcgt aagtgcgtac
    11641 gtagaaggtg cgccctcaag atgagcttca agcgcctcgc gggaaagaag cccctcctgg
    11701 gcacccaccg cctgaacaac agacatcgaa ttaattggtc cccagagaag cgcttcctcg
    11761 agtggcttgc cgagcgtaag cgctactgag acggttgagg caaatgaatc gcccgcacct
    11821 gtgcgctcat acggctcccg agtatctggg tacattggaa taaagtacac attcgtacca
    11881 tctgaagcat gtgcaccctc acgcccgtcg gttataacga ccgttttgag gccaagtccg
    11941 tgcatgccag aaagaagctc tttgatatcc tgctctcctt cgcgcttcaa gatacgagca
    12001 gcctcctcct tattacagaa gaaaagatcg gagcgtgcat agatgcgctt caggcgttca
    12061 gtacccatag ccatctggaa ggtgcccggc tggaagacga ggcgcatatg agggcgtgac
    12121 tcggcaaagt ctgcaatagc gtcgtaatag gtgtccggta cttcgccgag ggaagaaagg
    12181 tagagtgtct tgatctcagg gagatctttc gggaacgcat attcataggt ctcgtgcttt
    12241 acgagaatag tgcgctcgct ctcgaaccag agcacgtagt ggtagttggt cttcttgtcc
    12301 tcctggagcg taacgtacgt agtatcaaca cctttagagg aaaggctctc aagtacttcg
    12361 tttccgtacg catcctttcc tacattggtt ataaggctgc tcgaaagtcc aaggcgagca
    12421 gcagacactg cagcatttgc ggaattgcca acacccacaa ccttcgtgga ggactcgaat
    12481 gggatcttgt caccgaaccg catgcatatc tcgcaactgc catcatctat gttgcagtgt
    12541 gaccgggcgt ccttgagacg gatgaagtcg tcgataacgg tgtcgcctat ggcgaggaaa
    12601 tctatgctca tgccgcctag tgtagcatcc tggctacttc gggatctcaa tgcaccagta
    12661 ccggcttccg ggagtcccgc cggcggcgtc tgagcagtgc catccttggg ttccaggtcc
    12721 gtacgtgtct cctatgtagc ttgcgacaag gaagtaggag ccgtaatcgt agtaatagta
    12781 tggatagctg ctgtcgctga agttcgggat gctcgccata tatgtcggct cgagcgccga
    12841 actcagagcg gtatcagcca cgttccagtt actcggagca ggactgtttg cgggcgagag
    12901 gccggtagcc aggtaatagt tattgagtgc aagcgctatc tgcctgagtt cggctttctt
    12961 ctgggcctcg accgctcgta tgcgtgcggt attaagagag gcgaggacta ctgacgagag
    13021 cacgccgatg atggcgatca ccacgagaag ctcgatgagg gtaaatccgc gggacttcat
    13081 acaccttcac gacgctgcat ttgtataagc tctttatgcg tcgtgccttc aggctcaaag
    13141 ccgcctgcca cgagccatac ggtcactgca aggagtcctg ccaacgctgc gattacaatg
    13201 agcgcaatcg aggcctcgcg ctcatctttc gctaatcccc atcggataac gagcgcgtag
    13261 agcccgctga cccgcgtggt tcgacttgct gccggagcaa gtgtctgttc ctcttcgaag
    13321 gtcacattca tgtactaagt ataccaaccc cgacatcatg ctcgatgaag acgtgtatag
    13381 cgctcacggc cttgtgctac catctctgca tgactgatct ctcagatatt gcccgcggaa
    13441 tgtttgcgcc tggcaagggg atcctcgccg cggatgagag taatgagagt gcggatgaga
    13501 agcgattaaa gccgtttggt atagaaccag gtcctgaaat gcgccggaag ttccgggatc
    13561 ttctcctggc aacgccgggt atcgagcagt acctcacggg tgtgattctc tataaggaga
    13621 cgctcgagca ggagagtctt caggggatcc cgtttccgaa gcttcttctc gataaaggca
    13681 tcgctcccgg catcaaggta gacgagggcc tggacccact tgaggggagt ccggatgaga
    13741 tgataaccaa ggggctcctt ggtttacctg agcgtcttat cgagtaccgt gagcgccatc
    13801 aagcgagctt tacgaaatgg cgcgccgcta tccgcatcag cggagatcgt ctgccgacaa
    13861 agaaagcatt gcttgagaac gcgaagcgtc tcgcctccta cgcatgcgag gtgcagcgag
    13921 caggtatggt acccatgctc gagccagaag tgttgctcga aggaagccat agccgcctgc
    13981 gtgcacgcaa ggtacttgag gagtcgatga ccatactctt cgatacccta aacgaacagt
    14041 gcgctgacct ctccggcgtc atcgtgaaga cgtccatggc gctctctgga agcgaaaccg
    14101 gaaggatgga tactcctgag gaagtggctg aggatacgct tggtgcactc atggcgacgg
    14161 taccggcggg tgttggaggc attgtattcc tttccggggg ccagacgccg gaccaggcaa
    14221 ctgagaatct gcgtgcaatc tcaagactcg cgaagaaact caaggcacca tggccgctca
    14281 cgttcagcta tgcgcgtgcg ttccaggaag aagcgctcca tatatggaaa gggaaggacg
    14341 aaaacgtgga agccgcccgg gaggcgttcc ttgcacgtct taagaaggcg tccgaagcat
    14401 tggtataatt tggaggtatg gacgtccagt ttgatgagac ctcgtacgct cctcggacaa
    14461 cccaggctgc gcgcgtttct accatgacga gacttgttat aaaagcgggt ctcgcaaaag
    14521 atgagaaaga agcaaataaa gtcttcctct ggataatcgg cctatgcctg ctcatacctc
    14581 taggactctg gctcgtgcct gcactcatgc cagaccccga gattcaacct atagttttgc
    14641 caagacaatg aatcacaagc gcggcttcac cctcatcgag cttcttgtcg tgatcgccat
    14701 catcggcgtc ctatcttccg tggtgcttgc gtcacttaat acggcacgca cgaagtctcg
    14761 cgatgctgcc cgcgtggcaa ccatgaagca gatccagaat gccctcgcgc tctaccacct
    14821 tgatcgcggg tactacccgc ccatgaccga gaacgagtgt ggaggtactg aagggtatac
    14881 gacgagcaat aataatttta tgcagggtct tgttacgacc ggatacctac cctcgtatcc
    14941 catcgatccc gggaatctta gctgcggcat gcagtattac ccgagcggta acaacgcata
    15001 ctacttgttc taccggctcg aggctggacc tacagccccg gcaaccggct gtaatcagcc
    15061 tccaacctgg tactgcatcg cgggcggata gggcgtaacc accttgcctc aaggccccat
    15121 ttcaggcata ctccaggaac tatgcagcac aaccttaccg tttccccgcg tgatatcgtc
    15181 gggaagagtt ccggcaagct cgctggcgag ggccttatcc cggctgtcgt ctatgggccg
    15241 aagcaggagg ctctctctat ttccgttccg cttcttccgt ttgaggcagt acttcgccat
    15301 ggcggcgaga gcgccctcat tgagctttcc ggccttccaa agccaatgca ggttctcgtg
    15361 catgaccttg atcgcgatcc ggttacgcat gtaccgcgtc acgtagattt ctacgcggtt
    15421 gagaagggtg ccaaagtaca gcttatggtg ccgctctcat tcgtaggcga gtccatggca
    15481 ctcaagcagg gcgcgaacct cgtgaaggtg ctccatgaag tcgaagtcga gtgtgatcct
    15541 tcaaagatcc cgacggagat tgaggtagac accgcagcgc ttgctgctga gggcgaccag
    15601 atccacgttt ctgatatcaa ggctccttct ggtgtcgcta tcctcacgcc aggcgaggag
    15661 gttgctgcaa ttgcgcaggc ggttgaggaa gagtccgagg agagcgcaac gctcgacatg
    15721 gacgctattg aggtagagaa gaagggtaag gaggaagagg gcgagtaagc ctgaacgctt
    15781 catgtttctg aagaggcccg cgcggtaagt gcgggcctct tcatttcgtc ttctgatata
    15841 ctactaaccc aatgaagcga atcgttgtcg tcctttccgt gttcgccctc cttatcgccg
    15901 gtcccgcggc gatcctcgcg caagaactca ccgcaagcga gcgtgctgag ctcgagcgtc
    15961 agcttgcaga cctcgagcgt gaaatgcaaa agacacaggc gaccatcgac aatctctcgg
    16021 cgcagggcgc ctcgctgcag cgcgacatcg cgattctcga cggagagatc aagcgcgcgc
    16081 gccttaaggt gcagaccgca gaagcagaga tccgcgcgct cgcggccggg atcgtggtac
    16141 acacgcgcac catcggcacg ctttctgaaa agatgattcg tgagcaagat tcacttgcac
    16201 agattttgcg taagactgat gcgatcgacg actacaccct cgtcgaggca gtgctctcag
    16261 gcgccgagat atccgacttc ttcggggatc ttgatacctt cgcgtccatc aagcaggcga
    16321 tgagaacctc attcgaggag cttcgcgcta cgaaagcgga gacgcaggaa gagcgcgcag
    16381 ggctcgagag ccagaaaacg gaccaggaga tcctccgttc tgcgctcgta ctcgcgcagc
    16441 gcgaggttga agcgaaggaa gctgaaaaga agaagctcct tgccgaaacc aagggacagg
    16501 agagtttgta ccgaggactc aagtcctcgc aggagcagac ggccgcgcag atccgtgcga
    16561 agctgttccc gcttcgtgat acttcgggca ttcagttcgg cgacgcggtg cgctacgcgc
    16621 aggcagcaag cgcgcgtacc ggtgtgcgcc cggcactcat cctcgcgatt ctctcgcagg
    16681 aaagcgacct cggaaagaac gtggggcagt gtcttatcac ggaccttgca accggagatg
    16741 gtaagggcaa gaacacgggc acgccattcc caggcaccat gaaggcgcca cgcgataccg
    16801 tgccgttcga gcgcctcatg aaggcagcag ggcgcgatcc tttgaataca ccaatctcat
    16861 gtccacttcc gggcggctat ggcggcgcga tgggtccgac gcagttcatc ccatcaacct
    16921 gggaaatgta cgagggacgc attaaggccg cgctcggtgt gtcagcagct gacccgtgga
    16981 atgcgtcgca tgcggttatg gcaaccggtc tctatcttgc ggacgtgggc gcggcaggag
    17041 gaacgtacct cgcagagcac acggcggcag ctcgctacta cgccggcggc aactgggcta
    17101 cgagtggtca aggatacggc aatagcgtga tgagcaaggc cgctgccttc cagaaggaca
    17161 tcgacttcct cgaagcgaac tagtatcaac acgaaggaga ataggtgcga gtgcggatgc
    17221 tagtcttaag acacagcaga tatgtacaaa acactctttc tcttcttggt tgttggtatg
    17281 ggcgtagcgg cattcttcct cgctcctgat acggctaatg ctgcagcgtg taatttcacg
    17341 aacaccaaca taaacatcgc gtggtcgccg agtgatccct acgaccaaca gggaggttcg
    17401 ggtagttacg catatactcc aggtacggtc gtagctcgtt cctatggcga tctcagtctg
    17461 aacaatcaga caaacaggaa ctgggttaca acagttaccg ttacgaacaa cggagtcaca
    17521 agaacactca cgaacagcag ctgtacctgg gctgatatag acaacccgtg ctattcacag
    17581 acgggcgccc aagacgttgg cggtgctatg ttcaatctct attttgaggt gcagcactac
    17641 attaaggctg agttgacttg tacgccgtct ccggatgtaa cccctgacct cgtcggacag
    17701 gtaggaggcg cacgtaccgg acaggtgcag cagcccatga ccctctatgg cgccatcgca
    17761 aaccagggag gtgccgctgg agcgttcgag aatatcatcc aggtgtgcga tcagagctgc
    17821 tttacgctaa atcagatact ttctgctacc acgctctcat cacttggtgc cggaagctct
    17881 gctgctatat ctgccaacta cacgcctcct tcgggcatgg gaacgtcaga ctttatgtat
    17941 cgcgtgtgcg caaatacacg cgtggtgacg agtaagggat cgagtacgtg gagcaatcca
    18001 gcaactgaat cgaactatgc gaataactgt agtggctggc agtcgctcac catccagcag
    18061 gaaccggttc cgccgacagc gaacctcacg cttacaccaa cgctcattgc ctatggaggc
    18121 gcctcgattc tgcagtgggg ttctaccaac gcgacgtcct gcacgggtac taatttctca
    18181 actggaggcg cgacatcagg cagtctttca gttaccgcga cccaggacac cacctacaca
    18241 gtcacgtgta ccggccctgc aggaagcgca tcagattctg agcttctgag cgtcggcgca
    18301 cagccgcagc cagatcttgt tgcaggctcg gtgacggcag gaagtccgca ggcaggtgtc
    18361 tctgcaacgc tttcagcaaa tgccactaac gttggcccgg gcacctcagg tgcgttccca
    18421 attctattcc aggtatctga gaccggtgca ctcgcacaga gcggatacct cgcagcgctc
    18481 agttcgggag cgtcaggaag cggctctgcc tcctacacat tcccctcggc ggggacctac
    18541 caggttcgtg cgtgtgcaaa ctacaacacg agctggactg ctataacgac ggaatcaaat
    18601 tacgcaaaca actgcggacc gtggacgaat gttaccgttg cctctgcacc ccagccgcca
    18661 gccctttcgt gcagcgtgtc gcaggcttca gttactccgg gaggatcggt aacctatagc
    18721 gcaaaccctt caggcggcgc ttcaggcccc tacacctgga ctgcggccga cggcggcagc
    18781 tacggcaccg gttcgactgc atcgcgaacc ttcagcactc caggcaccta tgccatgaac
    18841 gttgatacgt cctcgactgc ggtg
//
