LOCUS       DASWYB010000001        10392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_359924, whole genome shotgun sequence.
ACCESSION   DASWYB010000001 DASWYB010000000
VERSION     DASWYB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359924"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1255)
                     /gene="dnaN"
                     /locus_tag="VGE59_00005"
     CDS             complement(140..1255)
                     /gene="dnaN"
                     /locus_tag="VGE59_00005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY1074072.1"
                     /translation="MNFSVTQENLVKGLQVVNRIATTRATLPVLGNILLTTDKGRLKL
                     VSTDLELAIDTYIGAKVEIEGAITVPARTFTDFVTNNSDTTIDFSLNDTVLNAKSAHY
                     TAEIKGIDATEFPTIPEIGGTNVIHLAAPLFKEAIQQTVFATTQDETRPVLSGVALFI
                     SGKELKLVATDSYRLAEKRIPIEEGEANDVTVIVPARALLELSRLIHDDVARVSLSID
                     QHQMMAVCGESHLVSRLIEGTFPAYEAIIPKELATTVTLNRSEFISSLKMASLFSRDS
                     AYNVTFTVGERDITLKALSAQIGASTSTVTAAVSGPAVSIAFNARFVLEVLQVMSCDA
                     IQFQLQQPHDNQWYPGIVKSTQDQNYQYVIMPLRTEV"
     gene            complement(1738..3102)
                     /gene="dnaA"
                     /locus_tag="VGE59_00010"
     CDS             complement(1738..3102)
                     /gene="dnaA"
                     /locus_tag="VGE59_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY1074073.1"
                     /translation="MTDTGIWQAVLGELEVTTSKAHFQTWLKRTKLHSINESEAVISV
                     PDVFVRSWVENHYKDQFLISFHKHGQTIESVRFLIDGSTAVPVVEMEITDHPKENQIK
                     YEQTRTPTLDEAPSSINPSRIFETYVEGNNNRLAYAVCKAVAQSPGTLYNPLFIYGGV
                     GMGKTHLMHAIANEVLKQDKSKKVLYVSSEKFASDYITGLASKSINKFKQTYRSLDLL
                     LIDDIQFIANKEGTQEEFFNTFNTLHQSNRQIVIAADRVPKAISGLEERLTSRFGWGM
                     VVDIQPPNFETRLAILKSKCTEKGVTLPEDALEYIATQIQSNIRELEGTLNRVVTYCM
                     MTQVPFSLESVRMALSGLITPVDRKTLSVETVLSVIAEHFGSTVDNLLGKRRNKELVY
                     PRHITMYILREQLDMSFPVIGRELGGKDHTTVMHGYNKICNDLENPRVQSDISRIKER
                     LASI"
     gene            3330..3479
                     /gene="rpmH"
                     /locus_tag="VGE59_00015"
     CDS             3330..3479
                     /gene="rpmH"
                     /locus_tag="VGE59_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY1074074.1"
                     /translation="MATKGIRTFRPKSDRRRVRVHGFFARVRANAGRILKARRLKGRK
                     RLTVA"
     gene            3484..3855
                     /gene="rnpA"
                     /locus_tag="VGE59_00020"
     CDS             3484..3855
                     /gene="rnpA"
                     /locus_tag="VGE59_00020"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY1074075.1"
                     /translation="MFKREQRLTDKRLFATLFRRGTWVRGRIMSFILMSTPHRGKIGF
                     VITKKVAKSAVVRNRTKRRIRATFIDMLKLPEYKEFLLDTTVAVMIHRTIDELSYEQL
                     KSETVHLFEKLKKADPKSKLS"
     gene            3852..4082
                     /gene="yidD"
                     /locus_tag="VGE59_00025"
     CDS             3852..4082
                     /gene="yidD"
                     /locus_tag="VGE59_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY1074076.1"
                     /translation="MKKIIIGSIRLYQRTLSPDHGIFRRPTCRFTPTCSEYTIEAIEN
                     HGIIAGVALGVRRISKCHPLTEGGYDPVPPRK"
     gene            4085..4852
                     /locus_tag="VGE59_00030"
     CDS             4085..4852
                     /locus_tag="VGE59_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEY1074077.1"
                     /translation="MKEFFKVILYQPLFNLFIAILAITPGHSVAVAIIIITFLIRLLL
                     TPIKYKSIESQLKQREIQPEIKRIQEKYKDDRQAQSTAMMQLYKDKGVNPASGCLPMI
                     VQLALLISLYSVFRHGWGPSQESVLYSFVHLPPYINSAFFWVKDLTQADHTLVLPVLA
                     GIAQFFYTRSMMASMPTPVSTGSDDMSAIMSKQMMYLGPAFTILIGRTLPAALTLYWL
                     VGTLVDWYQQVHVAKRVNHNLGNKVSVSVRTKKKETL"
     gene            4849..5298
                     /locus_tag="VGE59_00035"
     CDS             4849..5298
                     /locus_tag="VGE59_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY1074078.1"
                     /translation="MTELQLHEIVTEMLAHLGLAINAEISQDGDVYLINLKGSDCRFL
                     ERGKDNRQGAFVTLVKLIAKQRYDSDPRLILDFNGQRSQRLENIAQLARKKAEMVRVS
                     GQEEEMPPMTPAERRAVHVALKEMTGIRTESRGVEPHRRIVIILDEE"
     gene            5371..5462
                     /locus_tag="VGE59_00040"
     tRNA            5371..5462
                     /locus_tag="VGE59_00040"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5407..5409,aa:Ser,seq:cga)
     gene            complement(5679..6020)
                     /locus_tag="VGE59_00045"
     CDS             complement(5679..6020)
                     /locus_tag="VGE59_00045"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEY1074079.1"
                     /translation="MKAISLKQPYANWIAEGKKTLETRTWATKHRGNLLIVSSRLPAL
                     EPAGYAVAVVEVIACRPMTEQDEQAACVSLYPKAWVWELAHIHRIKPIPLKGSLGIYS
                     VPIEISDLEYI"
     gene            complement(6017..7642)
                     /locus_tag="VGE59_00050"
     CDS             complement(6017..7642)
                     /locus_tag="VGE59_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY1074080.1"
                     /translation="MTLKRKTRFHYLLRVTVPSLFFIFVVATLSKNIPPLAATTSRIL
                     TVSPGGSTTSTVCSTSIPCRTIQQAIDLSTPGDTIEILPGVYKAFKVMNKSDLTIRAQ
                     SSDVTVLADPNLYSGALVVRIDNSHHITLEGISATNAASAGGMNGRGAFITLSNYITL
                     RNNTFLNNEDDGVTTGNSHNTLIEGNTFFNSTHHGLYISNSSDNITIRKNKLYNIAGS
                     ALQVNAAENGLSGEDGISTNVLIEQNTIFNSGQGMNLLGIQDSTIRNNLLWGNVGTGI
                     VLSRDDDACARAPEGPKNINVFHNTVIMGGGARYPIQIKESLGPIRIKNNIFSSIDTN
                     RGFLDIYNKTLTCQKTPSQDIGNTVSDYNIFTGPTARIALNDWQTFKSLAEWQALGKD
                     THSQTFSDTASLFSDGTSYRLSMSSPARNSGTTDAGVVTDIDDETRPSGSAPDSGSDE
                     FMEPDLTPLSITKVTSRSVVRSGKIISITLRYQNSNQVTVPSVTITDTIPSGFNYIPN
                     SSSGVLQGATLAWNLGSLAPQAEGTLTYQLINP"
     gene            complement(7645..8817)
                     /locus_tag="VGE59_00055"
     CDS             complement(7645..8817)
                     /locus_tag="VGE59_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1074081.1"
                     /translation="MRWIDIFRISVRMLRTNGLRSILTVLGIGVAISLIVILIGIGYG
                     LQNITIGSIVESKTLLSLDIQPPPNDVIPLTDETVKMVQSLPGIRDASPVITTVGEIR
                     IDNKLGSVTIVAAEPSYLDMEGIELKSGDAFSETGADVIVTPQVLELLDLGEGGIVGA
                     PAKLTYTDPSNESQSKAIDRITIVGIANNDTATIYVPYKLLASGGGVKFNLIKAVAND
                     RGSLVTARDALVQKGLLVETLIETLDQARTVFRWVTIGLTVFGTIALVVAAIGMFNTL
                     TISLMERTREIGIMKSIGVTNSSVKKLFLAESAILGLCGGLAGVLIGLALDQLLELFI
                     NQVAIRYGGVVLELFQYPQFFLLSMVLYPIILSVLTGLYPAMRASRLNPLRALRYE"
     gene            complement(8826..9842)
                     /locus_tag="VGE59_00060"
     CDS             complement(8826..9842)
                     /locus_tag="VGE59_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074082.1"
                     /translation="MKHPLISIQMVSKTYGEDALDFKNLVLKNVYLDIHEGEFLIIFG
                     PSGSGKSTLLNLMAGLEFPTAGRVMVRRKDLAKFTSEELARYHRLRMGMVFQQFNLIK
                     SLSVWENVALPQTASGVRYGLRKKRALKLLKTLNVDHYADRHPNEISGGEQQRVAIAR
                     ALINNPYFLLVDEPTGNLDSKSAEEVMKILEELHQTHRHTIVLVTHNPNHLRYATRIV
                     YVEDGKIVIEENREQLPDATASSNRELLNGDAPVTKPSAKTNHTAEEILQPIKNTTAA
                     QSDTSTTTPSTDTPAIVDDSSKDDIAHSSKNNPITSPSDKTAPIKVANKVATTPTSHP
                     AGKK"
     gene            complement(9847..>10392)
                     /locus_tag="VGE59_00065"
     CDS             complement(9847..>10392)
                     /locus_tag="VGE59_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074083.1"
                     /translation="AQDEKELSAGDTTLQGDHAVTLRNLYGLTDYTFKVLATDAQGKQ
                     VISSERKFTTLKDTTPPEIKDFKVSVTRSGEELVLTATWKTNEPTKSNVFYNPKTNTE
                     QVTELPESSAYLTEHVIVSSGLIPSTPYSLKAISSDFFGNQAEETINFVSPGIRKSIF
                     QLILDNILNQLGWVSKVFDRN"
BASE COUNT         2901 a         2134 c         2322 g         3035 t
ORIGIN      
        1 ttttcccgct gccattgggg ccaataaaaa gcgtgtgctt gcctaaagga aagccctgat
       61 ccgtatagtt acggtactgg atgagctgaa tatgtttcaa agatgaaatc ggaaccctcc
      121 caccgaacct aaatcttgtc tagacttcag tccggagtgg catgataacg tactgataat
      181 tttgatcttg agtcgacttt acaataccag gataccattg gttgtcgtgt ggctgctgaa
      241 gctgaaactg aatcgcgtcg caactcatga cttgcaacac ttctaagaca aatcgggcat
      301 taaaggcaat cgagactgcc ggaccactaa ccgcagccgt aaccgtcgac gtacttgccc
      361 caatttgagc tgataacgct ttaagggtaa tatctcgttc gcctaccgta aacgttacgt
      421 tataggctga atcacgcgaa aagagacttg ccattttaag actactgata aactccgacc
      481 ggttaagcgt cactgttgtt gcgagttctt tcggaataat agcctcgtaa gccgggaagg
      541 tcccttcgat aagccgagaa actaaatgac tttctccaca aactgccatc atttggtgct
      601 gatcaattga aagagaaaca cgagccacgt catcatgaat caatcggctc aattctaaga
      661 gagcacgagc aggcacgata accgttacat catttgcttc cccttcttcg ataggaattc
      721 ttttttctgc gaggcgataa ctatcggttg cgacaagttt taattcttta ccagagataa
      781 agagagctac accacttaag acaggacgag tttcatcttg ggttgttgca aacacggttt
      841 gctgaattgc ctctttaaaa agaggagcgg cgagatgaat aacattggtt ccaccgattt
      901 cgggaatagt aggaaactct gttgcatcaa ttccttttat ttctgccgta taatgggcac
      961 tttttgcatt gagaacagta tcgttcaaag aaaaatcaat tgtcgtatca ctattattgg
     1021 taacaaaatc ggtaaaagtg cgagcaggaa cggtaattgc tccttctatt tctactttcg
     1081 ccccaatata tgtatcgatt gctaattcta agtcagttga aactaatttc aatctgcctt
     1141 tatctgttgt gagtaaaata tttcccaata cagggagtgt ggctcgcgtc gtggcaattc
     1201 tatttacgac ttggagacct ttgaccaagt tttcttgggt tacagaaaag ttcataacaa
     1261 cctccgcttc tctcctccct ttctatcata ggtatgccat agatgctact tttccacaag
     1321 ccatacaaac acacctcgtg ttttatatta tgtaaatgtt aattaacaaa aacatattag
     1381 taatttataa atagatagta gtaataataa gggctgtgga aaacgtgaat aatcgtattt
     1441 tgaaccttat aaagatatgt gtatagctac taatatatgt ggggatgatt ttggaaaaga
     1501 atggggacaa aactaactgt ggaaaagtta tcgttagtta ttcacattct ttgtgcaatt
     1561 ctcccccatg ttttccacag gcttttaaca taatttatcc ccagaatgtg tggaaaacta
     1621 tctgtggata agtatgggga taagtgggaa aacttcttgg taacgaacca agaaagaaaa
     1681 agagatacac aaaagagtag gtgttcccta ctcttttact ttctttgatg gatatattta
     1741 gatacttgcg agtcgttctt ttatgcgcga gatatcactt tgaacacgag ggttttcaag
     1801 gtcgttgcat attttattgt atccatgcat caccgtggtg tggtcttttc cccctaattc
     1861 tcggccaata actggaaatg acatgtcgag ctgttctcga aggatgtaca ttgtaatgtg
     1921 acgagggtag acaagctctt tatttcgacg ctttccaagc aaattatcta ccgtggagcc
     1981 aaaatgctct gcaataaccg acagaacagt ttcaactgaa agagtcttcc ggtcgacggg
     2041 agttatgagg cccgatagag ccatacgaac tgactccaaa gaaaaaggaa cctgtgtcat
     2101 catacagtac gtaacaactc tatttaaagt accctcgagt tcacggatat tgctttgaat
     2161 ttgggttgca atgtattcta acgcatcttc cggtagcgtt acgccctttt ctgtacactt
     2221 actttttaaa atagcgagtc gagtttcgaa gttagggggt tgaatatcaa ccaccatacc
     2281 ccacccgaaa cgtgaggtga gtcgctcctc tagtccggaa attgctttcg ggactctatc
     2341 ggccgcaatg acgatctggc gattcgattg atgaagtgta ttaaaggtat taaaaaattc
     2401 ttcttgcgta ccttctttgt ttgcaataaa ttgaatgtca tcgatcagga gtaaatcaag
     2461 tgaacgatac gtttgtttaa atttgttaat actctttgaa gcaagaccgg taatgtagtc
     2521 gctcgcaaac ttttcgctcg agacataaag tactttctta gatttatctt gtttgagtac
     2581 ttcgttggca atagcgtgca taaggtgcgt ttttcccatc ccgactcctc cataaataaa
     2641 aaggggatta tagagagtac cgggactttg ggcgacggct ttgcatacgg cgtaggctaa
     2701 acggttatta tttccttcga catacgtttc aaatattcgc gacgggttga tagaagaggg
     2761 agcttcgtct agggtcggtg ttcttgtctg ttcgtatttt atttggtttt ctttcggatg
     2821 gtcggtgatt tccatttcta ccacgggcac tgccgttgaa ccatcaatta aaaagcgtac
     2881 ggattcaatg gtttgcccat gtttatggaa ggaaattaaa aactgatctt tataatggtt
     2941 ttctacccaa cttcgtacaa aaacatcggg gacgctaata acagcttccg actcgtttat
     3001 tgaatgaagt tttgtgcgtt taagccatgt ttggaagtga gccttgctcg tagttacttc
     3061 gagctcacct aggacagctt gccaaattcc tgtgtcagtc acgatcctcc ttgcggtcgt
     3121 ctcctctact tcatagcagg ttctcataaa gttatccaca gtcgatacca ccatgccatg
     3181 aaagtttttc ttccgtcaag cagatatgga gaaaagcaag caattgccct gtggaaaagt
     3241 tgtaagaata tgtaaagtaa ggtaaaataa gacagatata ataaggaaat ttccttagtc
     3301 tttcacgcaa aaacatagag gagcgtagta tggcaactaa aggtatccgt accttccgcc
     3361 caaaatcaga ccgtcgtcgc gttcgtgtgc atgggttctt tgctcgtgtt cgcgccaacg
     3421 ctggtcgtat tcttaaagcc cgtcgtctca aaggacgcaa gcgcttaacc gtcgcttaat
     3481 tatatgttca agcgcgaaca acgtctcact gataaacgtt tgtttgcgac tctctttcga
     3541 cgtggaacat gggtgcgggg tcgtattatg agttttattc tcatgtcgac tcctcatcgc
     3601 ggcaaaattg gattcgtgat cacaaaaaaa gtagccaagt cagctgtcgt acggaatcgc
     3661 actaaacgac ggattcgggc tacttttatt gatatgctta aactgcctga gtataaagaa
     3721 tttctcttag atacgaccgt agcagttatg attcaccgaa ctattgatga attatcctat
     3781 gagcaactta aaagtgagac ggtacatctt tttgagaaat taaagaaagc agatccaaaa
     3841 tcaaagctgt catgaaaaaa atcattatag gaagtattcg tctgtatcaa cgaacacttt
     3901 ctccagacca tggcatattt cggcgaccta cctgtcgttt tacccccacg tgctccgagt
     3961 acacaattga agcaatcgag aatcatggta ttattgcagg agtcgctctg ggtgttcgtc
     4021 gtataagtaa atgtcatcca ctcacagaag ggggttacga tcctgtccct cctagaaagt
     4081 aagtatgaaa gaatttttta aagttatcct ttatcagcca ctcttcaatc tttttattgc
     4141 tattttagcg attaccccgg gacacagtgt ggcggtagct atcataataa tcacgttcct
     4201 gattcgtctt ctcttaacac cgattaagta caaatctatt gaatcgcaac ttaaacaacg
     4261 cgagattcaa ccagaaatca aacgaattca agaaaaatat aaagatgatc gccaagccca
     4321 aagcacggcg atgatgcagc tctataagga taaaggagtc aatccggcga gtggctgttt
     4381 accaatgatc gtacagttag ctctccttat ttctctgtac tcagtattcc gtcatggatg
     4441 ggggccgtcg caagaatcag ttttatattc cttcgtccac cttcccccat atattaatag
     4501 tgcttttttc tgggtaaaag atttaacgca ggctgatcac acactggtac ttcctgtttt
     4561 agcaggtatt gctcagttct tttatacacg cagcatgatg gcatcgatgc cgacaccggt
     4621 gagtacgggc agtgacgata tgtcggccat tatgagtaag caaatgatgt acctcggacc
     4681 agccttcacc attttaattg gacgaacact cccagccgcc cttactcttt actggttagt
     4741 cggaactctt gttgattggt atcaacaggt tcacgttgca aaacgagtca atcacaatct
     4801 tggtaacaaa gtatctgtct cggtccgtac gaagaagaag gagaccctat gacagaatta
     4861 cagcttcatg aaatcgtaac cgagatgctg gctcatttag gtttagcgat caacgccgag
     4921 atttctcaag atggggatgt ctaccttata aacttaaagg gtagtgattg tcgttttttg
     4981 gaacgaggaa aagataaccg tcagggagca tttgtcacac tcgtaaaact aattgctaaa
     5041 cagcggtatg atagtgatcc tcgcctgatc cttgatttta atggtcagcg ttcacaacgc
     5101 ttagaaaata ttgctcagct cgcgcgtaaa aaagctgaga tggtccgggt gagtggacaa
     5161 gaagaagaaa tgccaccgat gacccctgct gaacgccgcg cggttcatgt tgccctcaaa
     5221 gagatgactg ggatacgaac agaaagccga ggagtcgaac ctcatcgacg aattgtgatt
     5281 attctcgatg aagaataaga atgccccccg ttttgccggg gcattttcaa tctgagggaa
     5341 aaagggtata atttgcagag ataatactct ggagaggtcg catagcccgg ttgattgcgc
     5401 cacactcgaa atgtggtata cccgcaaggg tatcgtgggt tcaaatccca ctctctccgc
     5461 caaaacaaag acgccctcaa aagggcgttt ttgttttggt aataaatagt caggggattt
     5521 gaagggtatc cgaagcgctg ccgggaaggg cagatgaagt gaggaacccc ggttgcgaag
     5581 gaggtgcaat tacttgagcc ggacgagcaa aaatcccact ctctccgcca gacgaaaata
     5641 tcccctaaag ggatattttt cgtttatacg gtaagctttt aaatatattc aaggtccgag
     5701 atttcaatgg gtacagaata aattcctaat gaacccttga gaggaatggg tttaatccga
     5761 tgaatatgtg ccagttccca tacccatgct ttagggtaaa gggagacaca tgcagcttgt
     5821 tcgtcttgct ctgtcattgg acggcaagct ataacttcga caacggcaac tgcgtaaccg
     5881 gctggttcta acgcaggtaa tcgcgacgat acaatgagta agttgccgcg gtgttttgtg
     5941 gcccacgtcc tggtctccag tgtcttcttt ccttcggcaa tccagttggc atacggctgt
     6001 tttaaagata tggctttcat gggtttatta gttggtaggt gagtgtccct tcggcttgag
     6061 gggcgagtga ccctaggttc cacgctaagg tagcgccttg aaggacgcca ctacttgagt
     6121 taggaatata attaaatcct gaagggatcg tatcggtaat tgtaacactt ggcacagtta
     6181 cctgattgga attttggtat ctgagggtaa tggagataat ttttccacta cggacaactg
     6241 atcggcttgt aacttttgtg attgagaggg gagtaaggtc tggttccata aactcatcac
     6301 tgccactatc cggggcgctg cctgaggggc gtgtttcatc atcaatatcc gtcacgacac
     6361 ctgcatcagt agtaccgcta tttcgagcgg gggaactcat gcttaatcga tatgaagtgc
     6421 catccgaaaa gagcgaggct gtatcactaa aggtttggct atgcgtatct ttcccgaggg
     6481 cctgccattc agccaaagat ttaaatgttt gccaatcgtt caaagcaatg cgagcagtgg
     6541 gtccggtaaa tatattgtaa tcactgacag tatttccgat atcttgagaa ggagtttttt
     6601 gacaagtaag agttttgttg taaatatcta aaaatccacg gttcgtgtcg atacttgaga
     6661 aaatattatt ttttatacga atagggccaa gtgactcttt tatttgaatt gggtaacgtg
     6721 ctccccctcc cataataacc gtgttatgaa agacatttat atttttaggt ccttcggggg
     6781 cccgggcaca agcatcatca tctcgagaca atacaattcc ggtccctaca tttccccata
     6841 ataaattatt tcgaatggtt gaatcttgaa ttcccagcaa gttcattcct tggcctgaat
     6901 tgaagatggt attttgttca atcagaacat ttgttgaaat accgtcttca ccactaaggc
     6961 cattttctgc agcgtttact tggagagctg atccggctat attgtaaagc ttatttttac
     7021 gaatagtaat attatcgctg ctattactaa tatagagtcc gtggtgcgtg ctattaaaaa
     7081 aagtgtttcc ttcaataaga gtgttatgcg aatttcctgt tgtgacacca tcgtcttcat
     7141 tattgaggaa tgtgttattt cgaagagtga tgtaattact taatgtaatg aacgcaccgc
     7201 gtccgttcat acccccagcc gatgctgcgt tagtggcact aattccttca agggtaatat
     7261 gatgtgaatt atcaatgcgg acaacgaggg cgccactata aaggttcgga tcagcaagaa
     7321 cagttacgtc agaactttga gctcttatag tgaggtcact cttattcata accttaaaag
     7381 ctttatagac cccaggaaga atttcaattg tatcgcctgg tgttgataga tcgatagcct
     7441 gctgaatggt tcgacagggg atcgaagtag aacacacagt gcttgtcgtt gaaccacctg
     7501 gtgacaccgt gagtattcgg cttgttgttg ctgctagagg cggaatgttt ttagatagtg
     7561 tcgctacaac aaaaataaag aaaagagaag gaaccgtgac gcgtaagagg tagtgaaaac
     7621 gcgtttttcg ttttaacgtc ataattactc ataacgcagt gctcgcagcg gattgagacg
     7681 agaagcacgc attgctggat agagtcctgt tagaacagac aatataatgg ggtagagaac
     7741 catactaagc aagaagaact gaggatattg gaaaagttcc aatacgactc ccccatagcg
     7801 gattgccact tggttaatga aaagctcaag taactggtcc agggcaaggc caatgagtac
     7861 tccggccagt cctccgcaaa ggccaaggat agcggattca gctaagaaaa gttttttaac
     7921 ggaactgttg gttaccccaa tggatttcat aattccaatt tcacgggtcc gctccatcag
     7981 ggagatagtt agagtgttaa acattccaat tgcggcgacc acgagggcaa tggtaccaaa
     8041 tactgttagt ccaatagtta cccaacgaaa aacagttctg gcttggtcaa gcgtctcaat
     8101 taatgtttca actaagagac ctttttgcac taaagcgtcc cgggcggtga ctaatgatcc
     8161 tcgatcattt gcaacggctt taataaggtt aaatttaacc cctcctccac tagcaagaag
     8221 tttataaggt acataaattg ttgcggtatc attattggcg ataccgacaa tggttattct
     8281 gtcaatcgct tttgattgac tttcattact tgggtcagta taggtcagtt tagcaggagc
     8341 acctacaatg cctccttccc ctaaatcaag aagttcaaga acttgaggcg taacaatcac
     8401 gtctgctcct gtttcgctaa atgcgtcacc gctctttagc tcaatccctt ccatgtctaa
     8461 gtaagatggt tcggccgcaa caatggtaac agaaccaagt ttattatcga tgcgaatttc
     8521 ccctacagtt gttatgacag gagaagcgtc tcgaatgcca ggtaaggact gaaccatttt
     8581 gacggtttcg tcagtaagag gaataacatc atttggcggt ggttgaatat cgagcgacag
     8641 aagtgttttt gattcaacaa ttgaaccgat tgtaatattt tggaggccat aaccaatacc
     8701 aattaagata acaatcaaac taatcgcaac tccaatacct aagaccgtaa gaatacttcg
     8761 taacccattt gtacggagca ttcgcacgct gatgcggaaa atatcaatcc atctcatagt
     8821 aaaccttact tcttccctgc gggatgggat gtgggagtcg tcgctacttt atttgctacc
     8881 ttaatcggtg ctgttttatc agacggcgag gttatagggt tatttttgct agaatgggca
     8941 atatcatctt tcgaagaatc atcaacgatg gcgggtgtat cagttgaagg agtagtcgtt
     9001 gacgtatctg attgtgcggc ggtagtgttt ttgattggtt gtagaatttc ttcggcggtg
     9061 tggtttgttt ttgcagacgg ctttgtgaca ggagcatcac cattaagaag ttccctgtta
     9121 cttgatgcag tagcgtcagg aagttgttca cgattctctt cgatcacaat tttcccatct
     9181 tcgacgtaca caattcgggt tgcgtatcgc aggtggttag ggttatgggt aaccaacaca
     9241 atggtatggc gatgggtttg atgaagctct tcaaggatct tcataacttc ttcggcagat
     9301 ttactatcaa ggtttcctgt tggttcatcg actaaaagaa agtacgggtt attaataagg
     9361 gcgcgggcaa tcgcaacacg ttgttgttcc cccccagata tttcattggg atgccggtcg
     9421 gcgtagtgat caacgttaag ggttttaagt agtttaagag cccgcttttt tcgcaagccg
     9481 tatcgaacac cactggcggt ttgagggagt gccacatttt cccacacaga gagcgattta
     9541 ataaggttaa attgttgaaa taccataccc attcgtaatc ggtggtaacg agccagttct
     9601 tcggaagtaa acttcgctaa atctttacga cgcaccataa cacggccagc tgtggggaac
     9661 tcgagtccag ccataaggtt aagaagggta gacttaccac ttccggaagg cccgaagatg
     9721 atcaggaatt caccttcatg aatatctagg tagacgtttt ttaaaaccaa gtttttgaaa
     9781 tccaaagcat cttcgccgta ggttttcgat accatctgaa ttgaaataag tgggtgtttc
     9841 ataggattag tttcggtcga agactttact gacccatccg agctggttta agatattatc
     9901 caaaatcaat tggaaaattg attttcggat accaggcgat acaaagttaa tggtttcttc
     9961 ggcttgatta ccaaagaagt ccgaagaaat tgctttgaga gagtaggggg tagaggggat
    10021 taacccactc gaaacaatca cgtgctccgt caagtatgcg cttgattcag gaagttcagt
    10081 cacttgctcg gtattggttt ttggattata aaacacgtta ctctttgttg gttcgttggt
    10141 tttccaagtt gcagttaaaa ctaattcttc gccagaacga gttacgctga ctttaaaatc
    10201 ttttatctca ggcggagttg tgtctttaag agttgtgaat tttctttcgg aagaaataac
    10261 ttgttttcct tgtgcatcag tggcaaggac tttgaatgta tagtcggtta aaccatacag
    10321 gttacgtagt gttacggcgt gatctccttg gagtgtcgta tctccggcag aaagttcttt
    10381 ctcgtcttga gc
//
LOCUS       DASWYB010000002         5051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_54770, whole genome shotgun sequence.
ACCESSION   DASWYB010000002 DASWYB010000000
VERSION     DASWYB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54770"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VGE59_00070"
     CDS             complement(<1..1428)
                     /locus_tag="VGE59_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEY1074084.1"
                     /translation="MLFKRKPAHPPAPHGKPMPHPPVQGGQVRRPLSPGAPRPASVTN
                     QPGRAPQAVQPMQRPVAVPQTAQAIPRPIAINRPQAPRVAQPHAPVPQRKDVRQERRR
                     LLAQQTEARHIFQEGLSTILDLIAPASLIVTPTHLRLNNRYVRTLFVYTYPRYVETNW
                     LSSVINYDVEFDISMFIYPQDTSQVMSSLRRRATQLESTETVEQERGLIRDPELETAI
                     TDIEQLRDVLQRGELKLFHFGLYISVYANSEADLNEIVKHMESILGGMLIYTKQSLLQ
                     MEQGFNSTLPLMQDQLKITRNLDTASLSTAFPFTSSSLTSNEGVLYGINRHNNSLVLF
                     DRFNLENANSVVFAKAGAGKSYTVKLEALRSLMVGVDVIIIDPENEYEKLAEAVGGSY
                     IRLSVDSPTRINPFDLPASTGVNMEGDGLRSTVAMLHGMVSLMVGGLSPEEDALLDHA
                     LYETYALKDISTDPATQKNPPPLLTD"
     gene            complement(1418..2110)
                     /locus_tag="VGE59_00075"
     CDS             complement(1418..2110)
                     /locus_tag="VGE59_00075"
                     /inference="COORDINATES: protein motif:HMM:NF022580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HEY1074085.1"
                     /translation="MASTPKPASTQKHLSIAQVRDSLVVMKDGTFRAVLLVSAVNFAL
                     KSQQEQDAIIYQFQSFINGMTHPLQIVVQSRQLDLNRYLLHMADLAQNQSNELLRFQM
                     VDYIDFVSRLVTLANIMEKRFYVVIPHYTVQTGGHGLLDMLTGGRKTVPHYSQEQFNH
                     YKQEITERVNVVVSGLSSIGLRAVQLNTQELVEFYYSIYNPEEATEEKLIDIEELHAS
                     IIQRKPGGSHAV"
     gene            complement(2113..2616)
                     /locus_tag="VGE59_00080"
     CDS             complement(2113..2616)
                     /locus_tag="VGE59_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEY1074086.1"
                     /translation="MQFKVPQNIDMADRIVGPLTLIQFLYLLIGGLIVYTLFSVIAPV
                     NTVLFVVTAGPIALLTLALTFLKVQDQPFPRFALAFILFLFSPKMRVWHREGPDPLLV
                     ITADPAKAKPQAPHKKIEKSELEKLSQILDTGGSKPAQAPELPHAVSPPNYLKRSRPV
                     LDAMKRK"
     gene            complement(2707..3615)
                     /locus_tag="VGE59_00085"
     CDS             complement(2707..3615)
                     /locus_tag="VGE59_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074087.1"
                     /translation="MSLMPAEQEFLEHTERSADEYPLEWREHIEAWPEEHRDHLVKIL
                     EANAERGDFSPELYRELGVTFEAVDKDEEMTYRGFEGRYKAAEAYTRSRRVRVFPELQ
                     RYSERMVGHVLRHELGHFLGEHCIDPSQYFSFVSHRPNWANSRYIRQVSQGDETLTVG
                     QIMHESSPELFLHEMLADDIADYLGAKTPEEMLASRLERAGSQADIEMWAQTYPEDFA
                     YLEEEASDLFLFFQESFSRSAEERLADAPGLAAEWRRFNEVEDDDDEDPSVAVTSEVA
                     QPLWSPPAPRPPKKEGTGFFSWLFGL"
     gene            complement(3618..>5051)
                     /locus_tag="VGE59_00090"
     CDS             complement(3618..>5051)
                     /locus_tag="VGE59_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074088.1"
                     /translation="NTLTEMNRSQETTTSTTSTSTSEAERRILTAREMPWAANTQGDK
                     PEDTIGGNPEAAPGGEKPKIDLANDLSGLVVQGNATVQGDAHFDNPAVAESFAKLAAM
                     YGGPGEDAFRMMQQFYGGQKTPDQKELAQEAPRPWMAALKEGIDAAAEAAMNAGRDHG
                     FEEEEMANAMQLVRDRSDVGTEVSAEDLKAIADGKEWGMVMPELSSYGKKYGDYKKGI
                     DMAIPEDMQITEKAALELVPELDFDHTKFAESLENSRRFLDDSLRRLANDTLERDGLR
                     AIKDRLGRIYKPAALIPDDVDNVGMQIQLRKMATRTRDAVSLLQDKTMTDAFSRGVKG
                     QDLKVALRNTVRVRAIEDKTVLDTANLVDKIKRDIGETDPIKVATNQDVIHSVSANIK
                     TMVDVHPQLRASIAQVSPSQHDMIHQQAAQEFTQAFAKQVQQSDSALKSVYPTETRQQ
                     IGSQLGEIVSRNIQQAIRTNGMQPAVA"
BASE COUNT         1287 a         1228 c         1195 g         1341 t
ORIGIN      
        1 atctgtcagc agtggcggcg ggttcttttg ggtagccgga tcagtcgaaa tatctttgag
       61 cgcgtaggtt tcataaagcg cgtggtcaag taaggcatct tcttccgggg aaagtccccc
      121 gaccatcaaa gagaccatgc cgtgcaacat tgcgaccgtc gaacgcaatc catcgccttc
      181 catatttacc ccagtacttg cgggtaaatc gaaagggtta atccgggtag gagaatcgac
      241 agacagacgg atataactgc ctccgaccgc ttcggccagt ttttcgtatt cgttttctgg
      301 gtcgatgata attacatcta caccgaccat taaactacgt aacgcctcaa gttttacggt
      361 gtacgatttt ccagctccag ctttggcgaa aacaactgag ttagcatttt ctaagttaaa
      421 ccggtcaaag agaacgagtg aattattatg gcggttgata ccgtagagaa ctccttcatt
      481 agaggttaaa gagctggagg taaacggaaa cgccgttgaa agcgaggctg tatcaaggtt
      541 gcgagtaatc ttaagctgat cctgcatcag tggcagagtg ctattaaacc cttgttccat
      601 ctgcagaagc gattgcttgg tgtaaatgag cataccaccc aatattgatt ccatgtgctt
      661 cacgatttca tttaagtcgg cttcgctatt agcatagaca gaaatatata atccaaagtg
      721 aaacagcttt aactcacccc gctgcaagac atcacgcaat tgctcaatat ccgtaatcgc
      781 cgtttcaagt tctgggtcac gaattaatcc tcgttcttgc tcgacggttt cggtcgattc
      841 aagttgagtt gcccgtcggc gcaaactcga catcacctgg ctcgtatcct gcggataaat
      901 aaacatactg atatcaaact caacatcata gttaataact gaagacaacc agtttgtctc
      961 gacgtaccga ggataggtat acacaaacag ggtgcgaaca taccggttat taaggcggag
     1021 atgggtcggc gtcacaatta aagaagccgg agcaatcaaa tccagaattg tcgacaaccc
     1081 ttcttggaaa atgtgacgcg cctcggtttg ctgagcgaga agtcttcgac gctcttggcg
     1141 cacatcttta cgctgtggga caggggcatg aggttgtgcc acccggggag cttggggacg
     1201 gttaattgca atcggccggg gtatcgcttg ggcagtttgg ggaaccgcta ccgggcgttg
     1261 cattggctgc accgcctgag gagcacggcc tggctgattc gtcaccgagg caggacgtgg
     1321 tgcccccgga gaaagaggac gccgtacctg gccaccctga acaggaggat ggggcatagg
     1381 ctttccatgg ggagccggag ggtgggcagg cttccgttta aacagcatgg gaacctcctg
     1441 gttttcgttg aataatgctg gcgtgaagct cttctatatc tatcaatttt tcttcggttg
     1501 cttcttcagg gttataaatt gagtaataaa actctactaa ttcttgggtg ttcagttgaa
     1561 ccgcacgtaa gccgatactg gaaaggccac tcaccacaac attcacccgt tccgtaattt
     1621 cctgtttgta gtgattaaac tgttcttggg aatagtgggg aaccgtttta cgtccaccag
     1681 tcagcatatc taagagtccg tggccgcccg tctgaacggt atagtgagga ataacaacat
     1741 aaaaacgttt ttccatgata ttggctaagg tgacaagtcg gcttacaaaa tcaatatagt
     1801 caaccatctg aaaacgaagc agctcattcg actgattttg agccaagtct gccatatgaa
     1861 gtaagtagcg gtttaagtct aactggcggg attgcaccac aatctgtagc ggatgcgtca
     1921 ttccgttaat aaagctctga aactgataaa taattgcatc ttgttcctgc tgtgacttaa
     1981 gggcaaagtt aaccgcactc actaagagga cggctcgaaa cgtgccgtct ttcatgacga
     2041 cgagagaatc gcgcacttga gcgatggaga gatgtttttg cgtactggca ggtttagggg
     2101 tagaagccat acttatttcc ttttcatcgc atcgagaacg ggccgtgagc gtttgaggta
     2161 gtttggaggc gaaacagcgt gtggcaactc aggggcttga gctggtttgg agcctcccgt
     2221 atcaagaatc tgagacaact tttcaagttc agatttttca atttttttat gaggcgcctg
     2281 tggctttgcc ttggctgggt cagccgtaat cactagtaac gggtcaggtc cctcacgatg
     2341 ccacacgcgc atcttgggcg aaaagaggaa gagaataaat gccagtgcaa aacggggaaa
     2401 cggctgatcc tgtactttca aaaatgtcag tgcaagcgtt aaaagagcaa tcggtccagc
     2461 agtcactaca aaaagcaccg tattaactgg agcgataact gaaaaaaggg tataaacaat
     2521 aagcccacca atcaagaggt acagaaattg tatgagtgtc agaggcccca caatacggtc
     2581 ggccatgtca atattttgcg gtaccttaaa ctgcacaacc ctccttcgtt tctcgcgata
     2641 attgcaagaa attccgtagt tctaccataa cacgaactac tcaggtgaaa caatcgatgc
     2701 acaaagctat aggccaaaga gccaagaaaa gaatccagta ccttcttttt ttggtgggcg
     2761 aggggcagga ggagaccata acggctgtgc cacttctgaa gtaaccgcaa cggaaggatc
     2821 ttcgtcgtca tcatcttcga cttcgttaaa ccggcgccat tctgcagcca aacctggtgc
     2881 gtcagccaga cgctcttcgg ctgaacggct gaatgattct tgaaagaaga ggaaaaggtc
     2941 actcgcttcc tcttctaaat aggcaaaatc ctccgggtat gtttgagccc acatttcaat
     3001 gtctgcttga ctccccgcgc gctccaaacg agatgctaac atttcttcgg gtgtttttgc
     3061 gcccaaataa tcagcaatat catcagccag catttcatgc aaaaatagct cggggctcga
     3121 ttcgtgcatt atttgaccaa ccgtgagcgt ttcgtcgcct tgactcacct ggcgaatata
     3181 gcgactattc gcccaattcg gccgatgtga aacaaaagaa aaatattggg aaggatcaat
     3241 gcagtgctca ccgaggaaat ggcccagctc atgccgaagc acatggccga ccattcgctc
     3301 actgtagcgt tgcaattccg gaaatacccg gacacgacgg gatcgtgtat aggcttcggc
     3361 cgctttataa cgaccttcga aaccacggta ggtcatttct tcgtctttat cgacagcttc
     3421 gaaagtaacc ccaagttctc gatataattc aggcgaaaag tcgccccgtt cagcatttgc
     3481 ctcaagaatc ttcaccaaat gatcacgatg ttcttccggc cacgcctcaa tgtgttcccg
     3541 ccactctaag gggtactcgt cagcagaccg ttcggtatgt tcgagaaatt cttgttcagc
     3601 gggcatcaga gacattttta agctactgct ggctgcattc cattcgtgcg gatagcttgc
     3661 tggatattgc gcgagacaat ttcacccaat tgtgaaccta tctgttgccg agtttctgtt
     3721 ggatagactg attttaaggc cgaatcgctc tgttgcactt gcttagcaaa tgcttgtgta
     3781 aattcttgcg ctgcctgctg gtggatcata tcgtgctgac tcgggctaac ctgggcaatg
     3841 ctggctcgaa gttgagggtg gacatcaacc atcgttttaa tattcgccga cacgctatga
     3901 ataacatctt ggtttgttgc gaccttaatc ggatcggttt caccaatatc tcgcttaatt
     3961 ttatcgacta agttggcggt atcgagcacc gttttatctt caatcgcacg aacgcggacc
     4021 gtgttacgta acgctacttt caaatcctgc cctttgacac cgcggctaaa ggcatctgtc
     4081 attgttttgt cttgtaagag agaaaccgca tctcgtgttc gtgtcgccat ttttcgcaat
     4141 tgaatctgca taccaacgtt gtcaacatca tcgggaataa gcgcagctgg tttatagata
     4201 cgtcctaaac ggtctttaat tgcccttaac ccatcacgtt ccaacgtatc attggccaaa
     4261 cgacgtaatg aatcatcaag gaaacggcgg ctattttcga gagattctgc aaacttcgta
     4321 tgatcaaaat caagttcggg aacgagttca agagcagcct tttccgtaat ttgcatgtct
     4381 tcagggattg ccatgtcgat accttttttg tagtccccat atttcttgcc atatgaggaa
     4441 agctctggca taaccatacc ccattcttta ccatcggcaa ttgccttaag gtcttcagcc
     4501 gaaacttccg ttcccacatc cgaacggtca cgcactaatt gcattgcatt tgccatctct
     4561 tcttcttcaa atccatggtc ccgacccgca ttcatggccg cttctgccgc cgcatcaata
     4621 ccttctttga gtgccgccat ccacggccga ggcgcttctt gtgccagttc tttttgatca
     4681 ggcgtctttt ggcctccata aaactgctgc atcatacgga atgcatcttc gcctgggccg
     4741 ccatacattg ccgccaattt ggcaaaactc tccgcgacag ccggattatc aaagtgcgcg
     4801 tcaccctgaa cggtcgcatt gccttgcacc acaagtccag acaaatcatt cgccaaatca
     4861 atttttggtt tttcgccacc aggagccgcc tctgggtttc cgccaattgt atcttctggc
     4921 ttgtctcctt gtgtattagc agcccatggc atttctcgag cggtgagaat acgacgttcg
     4981 gcttcactcg tcgaagtaga ggtagttgac gtagttgttt cttgggagcg attcatttca
     5041 gtaagggtat t
//
LOCUS       DASWYB010000003        10382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_311627, whole genome shotgun sequence.
ACCESSION   DASWYB010000003 DASWYB010000000
VERSION     DASWYB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311627"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VGE59_00095"
     CDS             complement(<1..637)
                     /locus_tag="VGE59_00095"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEY1074089.1"
                     /translation="MPISPFMAAINQIADEKGLPKDSVIEAVEAALAAAYRKDYAHPE
                     NVIRARLSEDSEDVAMSQVFTVVTDEEFENEFHQMTLEEARKHNPKAEIGDEIEIAME
                     TQRDFGRIAAQTAKQVIIQRIREAERELLFSEFKDKEHQVVTGSVQQVDGDSVYVNLG
                     KISALMMQSDQLPSEQYYVGQRLKVFVTGVEETPRGPRVLVSRAHPELVSQL"
     gene            complement(829..1080)
                     /locus_tag="VGE59_00100"
     CDS             complement(829..1080)
                     /locus_tag="VGE59_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013327338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY1074090.1"
                     /translation="MAWFTYILECRDGTLYTGITTDLEKRVIKHNDGTAAKYTATRRP
                     VKMLWHESCADRSTATKRELEIKSLSRQEKLKLLSSGAE"
     gene            complement(1235..1543)
                     /locus_tag="VGE59_00105"
     CDS             complement(1235..1543)
                     /locus_tag="VGE59_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074091.1"
                     /translation="MLSTGYMIEVLFSLGGAMVILLHFIRKGEGGKRHQGLLKLTLVM
                     TISGWLYLAGLVLHRYDIVSHGVYLACSIASLSVMAAMAIAVIFCAFSAYQEPANLSA
                     "
     gene            complement(1682..2026)
                     /locus_tag="VGE59_00110"
     CDS             complement(1682..2026)
                     /locus_tag="VGE59_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074092.1"
                     /translation="MPDATVIVAMAGTFGFFLAACAMLLFVWECQMKKENQSAIWIAR
                     MALFLFTAVCLLSSVLLFILTNNEQRWEYGVLAILSSVASVIAGAASWLLHINYRAAY
                     RKPQPTPAAGKA"
     gene            complement(2077..2889)
                     /locus_tag="VGE59_00115"
     CDS             complement(2077..2889)
                     /locus_tag="VGE59_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY1074093.1"
                     /translation="MHKKFLDIPIDWVDPDALERKIKLFVTTKQPHHIVTVNPEFIVV
                     SRGNETFRTALQEADLSLPDGTGIVLGQTFFDKPYRKSKLTRLGQYLALCFQFVLYPH
                     SFTYKRITGVDLTDRLLQMSAAHGWRVYLLGANPGIAAKAAEVWTSVYPELKIVGTSS
                     SDPEDKNTIPDVKAAQPDILIVAYGSPKQELFIYKNKAALKVPVMVGVGGTFDTAAGT
                     RYNPPKWIKFLGFEWLTYLITQPKRYRRIWRSTITFSNLIISSKSLDKKLEI"
     gene            complement(2882..3982)
                     /locus_tag="VGE59_00120"
     CDS             complement(2882..3982)
                     /locus_tag="VGE59_00120"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY1074094.1"
                     /translation="MLIGIDASRALRKKRTGTELYCAEIIKYLTTIDHINRYRLYSSK
                     KPTGDMAQVKGSNIDWRVMPFTLGWTLIRLSLEILFHAPDVLFMTAHSIPLLPGKKSV
                     VIMHDIGFNHFPEIYKWIVKLYHRSTAIWIKHFATHIIVPTEFTKQDIHNQYGISLNR
                     ITVVPLGFEPKEYRPASKGETPPLDTPYFYYIGRIEMRKNISRLLEAFAEFKRQTGLP
                     HKLVLAGGPAYGYASIQATYENLGEYKKDVIMLGYTPQSKALEYMRHAEALVFPSLFE
                     GFGLPVLEAFASDTPVITSNTTSLPEVAGDAAILVTPTKTNEIADAMIKIAQDKEFRN
                     ELIQKGREQYKQFSWERTARETLAVLEKVGNA"
     gene            complement(4043..4936)
                     /locus_tag="VGE59_00125"
     CDS             complement(4043..4936)
                     /locus_tag="VGE59_00125"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY1074095.1"
                     /translation="MYADSHIEFIHHPTDLADLPNAAFLKTQINPTLINQIQPELLFT
                     AVYVDPGPNAWERTEWVIATYQECIRDNGWILVQSKADLNKLGIKIILHIEDLHMIGD
                     DLTKIKSLYDRGIRSIGLTHNHANQFAGGSLSPEVGLTDFGREAIKQLTELGMILDFA
                     HLSTQSFRDVATSFELAPFVSHTGLHGVYPTPRNFDDSLLSIVRDRNGYIGVGVAGSF
                     LGEKSARIQDVAQHILYAIEKTSPLNVGIGSDLGGTISFVPDGLTNISEVTNLLEYGI
                     NADVLGKNLIHFLARSPLRSE"
     gene            complement(5020..6396)
                     /locus_tag="VGE59_00130"
     CDS             complement(5020..6396)
                     /locus_tag="VGE59_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074096.1"
                     /translation="MKPIRIGVRTQDGLALATVESLLTGAPPRQDGSDGGPRRPRIFE
                     KINETTWRGEIHYGEGQYRVPVEVIIAPNGSYPTADWYVLSAGHIPCARQFTMHPRAT
                     RFLSDIPPGKHLVLSQQTHRGTYRDLDDPEKADTVPTPKVHKGQVASKAGRTLRQHFE
                     RLARNAANLLAAELTSQNVWRPYVLSFPYGDGARHFRTVAGWIQQETPLHLMSGICLH
                     ATECRSQIVQRLNERVIELLCRTAKCAFDPFPSIQGWTLSTFGPEDYEWLGDLGLSKV
                     LLPYMWDAPRGKVVADLKQNMALTQQVAQRLNGALRFPVTTAPLSSELGDIDLEWLTQ
                     AAEERAHVMLEAFGDSKPEIFKLMSPADQIARLRMEAFLYLLDTCRFGQVAGENRPAH
                     LYIGLEVHGGYWQKGNLSEGPKGEYLPLAWFNKCVGQHWGEWVLARNSTRQKEMMRLG
                     TMLQHLGY"
     gene            complement(6700..7020)
                     /locus_tag="VGE59_00135"
     CDS             complement(6700..7020)
                     /locus_tag="VGE59_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074097.1"
                     /translation="MTKKEQHPLWIQLGYSKSISAYLIGVFAGVQSYYAMSSVGFYLI
                     GGLVVALVAALIIALKSRTESNTWHDAWQEGITYFVLYVFSLTGTFLIAFILSNFLLP
                     FILK"
     gene            complement(7151..7438)
                     /locus_tag="VGE59_00140"
     CDS             complement(7151..7438)
                     /locus_tag="VGE59_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074098.1"
                     /translation="MTEKSEPPIDAVKHAKIWAGAFGASCGICSYIYTMPGWFIVAIG
                     AMALTLFGAWRFWNNAPETAQRYLPYIMMSLAFASFMAVRYVGSMFRIWIP"
     gene            complement(7577..7987)
                     /locus_tag="VGE59_00145"
     CDS             complement(7577..7987)
                     /locus_tag="VGE59_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074099.1"
                     /translation="MERKISVRMRGRVFGAPSLLYNNCLAPYRAVSRIDEFTYEGDPE
                     GLASAYAAWGQQDVEVWLPDRTRGTCDRRFLLEPDVEVTLNGNVLEGSIVRGYVHGDG
                     LGAFDFDAFLEAAAQPEGGAFMFRNLHLHPAKAA"
     gene            complement(8057..9064)
                     /locus_tag="VGE59_00150"
     CDS             complement(8057..9064)
                     /locus_tag="VGE59_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY1074100.1"
                     /translation="MVLRRLGIDLGTATVLVHVPQKGIVIQEPSVVAIETIDDKIVAI
                     GTAAKQMLGRTPDSIIAAHPLKDGVIANFRITEAMLRHYLNQLSGRIRLFKPDIMISA
                     PAGITSTERRAVMDACLGAGAKHAYLIKEPIAAALGANIPIASPSGNMIVDIGAGTTE
                     VAVVALGDIVASTSVRIGGNRFDHDIANYIRKKYNLVIGDQTAEDVKIKIGSVLPLDK
                     DLTLEISGSNAISGLPESILVTTSDVVNAIKEALNEIIGALKIVLQKTPPELASDIMD
                     KGIVMTGGSSKFRKIDELMTKVTGVPCQVADDPELCVVRGTGIAVEHLDEYRRSMLWT
                     K"
     gene            complement(9107..>10382)
                     /gene="murA"
                     /locus_tag="VGE59_00155"
     CDS             complement(9107..>10382)
                     /gene="murA"
                     /locus_tag="VGE59_00155"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY1074101.1"
                     /translation="KARLIMSVKYVIEGGHKLRGEITVNGSKNAALPMLAATLLTSEK
                     CTLHRVPMIRDIHVMLDILRHLGATVEIDGNTVSVETPHLVTTQIPDDLASKLRASVL
                     LLGPIIARAGKITLRHPGGCVLGKRPVGVHFDALQALGATFQQDEIKYYGQAAQLIGS
                     RMYLGEASVTATENAMMAAALAHGTTIIEPAACEPHIVSLGKMLNDMGAHVEGLGTHT
                     ITIRGAKSLNGTTAHVNADEIETGTLAVAFAITRGSGTIVGVEQENLSSIIYKLNQFG
                     VITEFEGNKLHIRSRETYKPTNIQINIWPLFPTDLQPQMTILATQAEGNSLIHDWMYD
                     RRLLYIDELVKMGGTIMLCDPHRALVTGPTKFSGKTIISPDIRAGMAFVLAGLIASGK
                     TTIEQAHLIERGYENIHERLNSLGAKIERIEN"
BASE COUNT         2450 a         2481 c         2726 g         2725 t
ORIGIN      
        1 agagctggga tacgagttct ggatgcgcgc gagaaactaa aacgcgaggt ccacgtggtg
       61 tttcttcgac gccagttaca aagactttaa ggcgttgacc aacatagtac tgctcgcttg
      121 gtaattgatc ggactgcatc atgagagcag aaattttacc aaggttcaca tagacggagt
      181 caccatccac ctgttggact gagccagtaa caacttggtg ttctttgtct ttgaattctg
      241 agaataagag ttctcgctct gcttcacgaa tgcgttgaat aataacttgc tttgcggttt
      301 gagcagcaat gcggccaaag tcgcgctggg tttccatggc gatttcaatc tcgtcaccaa
      361 tctcggcttt agggttgtgc ttgcgggctt cttctaaagt catttggtgg aattcgtttt
      421 caaactcttc gtccgtgaca acagtaaaaa cttggctcat tgcaacgtct tcggagtctt
      481 cagaaagacg ggcgcgaatg acgttttcag gatgagcgta atctttgcgg taggcagcgg
      541 ctaatgcagc ttcaacagct tcgataactg agtctttcgg aagacccttc tcatcggcga
      601 tttggttaat ggctgccata aatgggctaa taggcatagt gcgatcctct ctttaaatta
      661 tctttctgtc atctcgagct acgcgagaga tctgagttgg gtgtttttgg cctccagcct
      721 ggggtaaggg acaagggggc ttaattgcga agcagccccc tatccctcac cactcccaac
      781 ccctgcacgc taaagccaaa ttcaatacaa attaattcga caagcttctc actcagctcc
      841 agatgacagt aattttaact tttcttgtct cgatagactt tttatctcta gctctcgttt
      901 tgttgccgtt gagcggtcgg cgcagctttc gtgccacagc atttttacag gccgccgtgt
      961 tgccgtgtat ttggcggctg tgccgtcatt atgttttata acgcgctttt cgagatcagt
     1021 ggtaatgccg gtgtagagtg tgccatctcg gcattccaag atgtacgtaa accaggccat
     1081 tgacctcctg agggaacaaa aaaagcaagt gaaccatacc gtcacttact ctaaagaaat
     1141 aataccacgc ttttaaaaaa agtgaaaggc ggcatccgtg cgaatgagca cgaacaccgc
     1201 ctagagtctg gacatgaggt catggcctta cgatttaggc cgacaggttt gctggctctt
     1261 gatacgccga gaaggcgcag aagataacag caatcgccat tgcggccatc acgcttaggc
     1321 ttgcgatact gcacgcaaga tatacgccgt gagatacgat gtcgtaacgg tgcagcacta
     1381 gcccggcgag gtacagccag ccactgattg tcatcacgag cgtcagtttg agcagccctt
     1441 gatgacgctt ccctccttct cctttacgaa tgaagtggag aagtattacc attgctcctc
     1501 ctaatgagaa gagaacctca atcatgtatc cggttgacag catgttgctc ccccgtgtgt
     1561 cctcgatcaa ctcgcttacg gtatatttta tgtttcgttg actttgatgt caataaagtg
     1621 aaaggcggcg tccgtgcgaa tgagcacgaa caccgccttg gtttctgggg caggcgtggg
     1681 tttacgcctt accggcagca ggggttggtt gcggctttcg atatgccgct cggtagttga
     1741 tgtggagtag ccagctcgcg gctccagcaa ttacggatgc aacgctagag aggatggcga
     1801 ggacgccgta ctcccaacgc tgctcgttgt tggtgaggat gaaaagaagt acgctggaga
     1861 gcaagcagac ggccgtaaat aagaacagcg ccattcttgc aatccatatc gccgattggt
     1921 tttccttctt catctgacac tcccacacga acaggagcat cgcgcaagcg gcgaggaaga
     1981 acccaaaggt tcctgccatc gccacgataa cggtcgcgtc gggcatacag tttctccttt
     2041 gtggcgagcc caaacagtga cgttagtaaa tatatatcat atctcgagtt ttttgtcgag
     2101 tgatttgctg gaaatgatga ggttagaaaa agtaattgta gaacgccaaa tgcggcggta
     2161 gcgcttgggt tgcgtaatta agtaggtgag ccattcaaag ccgagaaact taatccactt
     2221 tggcgggttg tagcgagtgc cagctgccgt atcgaaggtt cctccgactc ccaccataac
     2281 gggtacctta agggcggcct tgttcttata gataaataat tcttgtttag gagatccgta
     2341 ggccacgatc aaaatatcag gttgagctgc tttgacgtca gggatggtgt ttttatcttc
     2401 cgggtcagac gaagaagtac ccacgatttt aagttcgggg tatacgctcg tccagacttc
     2461 ggccgctttg gctgcaatcc ctgggtttgc gcctaataag taaacgcgcc agccgtgagc
     2521 agcgctcatc tgtaggagtc tatcggttaa gtccacacca gtaatgcgtt tataggtaaa
     2581 tgaatgtgga tacaacacaa attgaaagca gagagctaag tattgcccta atcgggtaag
     2641 cttactttta cgatagggtt tatcaaaaaa agtttgccca agcacaatgc cggtgccgtc
     2701 aggtaaggag aggtctgctt cctgtagagc ggtacgaaac gtttcgttgc ctcggctcac
     2761 gacgataaat tctgggttaa cggttacaat gtgatgcggt tgttttgttg ttacaaaaag
     2821 cttgattttg cgttcaagtg catctgggtc aacccagtcg ataggaatat ctaaaaactt
     2881 tttatgcatt gcctaccttc tccaatacgg ctaacgtttc cctggccgtt ctttcccaag
     2941 aaaattgttt atattgctct cggccctttt gaatcaactc gtttcggaat tctttatctt
     3001 gggcaatttt aatcatggca tcagcaattt cattcgtttt ggtgggtgtt accagaatag
     3061 ccgcatcccc tgctacttcg ggcaggctgg ttgtattcga ggtaataact ggtgtatccg
     3121 aagcaaacgc ttcaagaacg ggcaagccaa atccttcgaa taaactggga aacacaaggg
     3181 cttcggcatg tcgcatatat tccagggctt tactctgggg cgtgtaaccc agcatgatca
     3241 catctttttt atattcacct aagttctcgt aggttgcttg gatgctggca taaccgtagg
     3301 ctggtccacc agctaaaacg agtttatgcg gtaatccagt ctggcgttta aattcggcaa
     3361 atgcttcgag tagccgagaa atgtttttgc gcatttcaat ccggccaatg tagtaaaagt
     3421 acggtgtgtc caaaggaggt gtttcccctt ttgaggctgg ccggtattct tttggctcga
     3481 atccgagtgg taccactgtt atacggttga gtgaaatgcc gtattgatta tgaatatctt
     3541 gcttggtgaa ttcagttggc acaataatat gagtggcaaa gtgtttaatc cagatagcgg
     3601 tagatcgatg atatagcttc acgatccatt tataaatttc gggaaagtgg ttaaacccaa
     3661 tatcgtgcat aatcacgacc gattttttgc cgggtaagag tgggattgag tgagcggtca
     3721 tgaacaatac gtcgggggca tgaaagagta tttccaagga caaacggatt aatgtccaac
     3781 ccagggtaaa gggcatgacc cgccagtcaa tatttgaccc ttttacctgt gccatatctc
     3841 cggttggctt tttgctggaa tacaagcgat agcgattaat atgatcaatc gtcgtaaggt
     3901 atttaataat ttcggcacag tagagttctg taccagtccg ttttttacga agtgcccggg
     3961 aggcatcaat cccaattaac atagatctat tttaacagaa gaaaaacaca aaagcactga
     4021 atattcgtga gtgactttgg agttattcag atctcagtgg agagcgcgcg agaaagtgaa
     4081 tgagattctt gcccaataca tcagcattga tgccgtattc gagtaagttc gtgacttcgc
     4141 taatattagt aagtccgtca ggaacaaacg atatcgtgcc gcctaggtcg ctgccgattc
     4201 ctacgttcaa tgggcttgtc ttctcgatcg cgtacaatat gtgttgcgca acatcttgaa
     4261 tacgagctga cttttcgccg aggaatgatc ccgctacgcc aactccaata tatccatttc
     4321 tgtcacgcac aatcgatagc aacgagtcat caaagttccg gggtgttggg tatacgccgt
     4381 ggagtcccgt atgggataca aacggagcaa gttcgaagga tgtggctaca tcacgaaaac
     4441 tttgcgtgct taagtgggca aagtcgagga tcattccgag ctcagtaagt tgtttgattg
     4501 cttcacggcc aaaatcagtc aggccaactt cggggctaag gctaccgccg gcaaactggt
     4561 tggcgtggtt gtgcgttagg ccgattgacc ggatgcctcg atcataaaga gatttgatct
     4621 ttgtcaggtc atcaccaatc atgtgtaggt cttcgatgtg gaggattatt tttatgccta
     4681 gtttgtttaa gtccgctttc gactgtacca ggatccagcc gttatctcgg atgcattctt
     4741 ggtacgtagc gatcacccat tctgtgcgtt cccatgcatt tggacccgga tccacgtaca
     4801 cagccgtaaa caaaagttcg ggctgaattt ggttaatcag ggttggattt atttgggttt
     4861 ttaaaaaggc tgcatttggt aaatcagcta aatcggttgg atggtgaata aattcaatgt
     4921 gagaatcagc gtacataata gttccttatg agtataacga aaaaagagtt ggcctgctcc
     4981 ccgaagggag caggcgcaaa cgaagggatg ggcgtactgc tagtagccca gatgttgcag
     5041 catcgtaccg agccgcatca tctctttttg gcgggtagag ttgcgggcga gtacccactc
     5101 cccccagtgt tggccaacgc acttgttgaa ccacgccaga ggcaggtact ctcccttcgg
     5161 tccttccgaa aggtttcctt tctgccagta gccaccgtgc acctcgagac cgatgtacag
     5221 gtgggccggc cgattctccc ctgctacctg gccgaatcgg caggtgtcca ggagatacag
     5281 gaacgcttcc atacggagcc gcgcgatctg gtcggcggga ctcatgagct tgaagatctc
     5341 cggcttgcta tcgccaaagg cttcgagcat tacgtgagcc cgttcttcgg cggcttgcgt
     5401 cagccactcc agatcaatgt ctcccagctc ggatgagagc ggggcggtag tgaccgggaa
     5461 gcgcagggcg ccgttgagtc gctgtgcgac ctgctgggtg agcgccatgt tctgcttgag
     5521 gtccgctacg accttgccgc gtggcgcatc ccacatgtag ggaagcaaga ccttggaaag
     5581 cccaaggtct ccgagccact cgtagtcttc cggcccgaag gtcgacagcg tccagccctg
     5641 gattgacggg aatggatcga aagcgcactt tgccgtccga caaaggagct cgatcactcg
     5701 ctcgttgaga cgctgaacga tctgcgaacg acactccgtg gcgtgcaggc agatgccaga
     5761 catgaggtgg aggggtgttt cctgctggat ccaccctgcc acggtgcgga agtgacgcgc
     5821 cccgtccccg taggggaacg aaagcacgta cggccgccac acattctggc tggtgagctc
     5881 ggctgctaac aggttagccg cattgcgcgc aagccgttcg aagtgctggc gcagtgtgcg
     5941 tccggccttg cttgctactt gtcccttatg cactttgggt gtagggacgg tatcggcctt
     6001 ctcgggatcg tccaggtcgc ggtaggtgcc tcggtgcgtc tgttgactta agacgaggtg
     6061 cttccccgga gggatgtctg acaggaaacg cgttgcgcga ggatgcatag tgaactgccg
     6121 ggcacacggt atgtggcctg ccgacagtac gtaccagtcg gccgtcgggt aggacccgtt
     6181 tggcgcgatg attacctcga ccgggacacg gtactgtccc tcgccgtagt ggatttctcc
     6241 tcgccatgtc gtctcattga ttttctcgaa gatacgagga cgacgcggtc ctccgtcgga
     6301 accatcctgc cggggcggtg ccccagtgag gagtgattca accgttgcaa gagctaatcc
     6361 gtcttgagtg cggactccga tgcgaatcgg tttcaaggta cattccccct atgaaagtag
     6421 cgttggtccg gataaaggct ttaggcctcg ccccggttag taataaagaa gtctaaaaaa
     6481 agaaccgcag gtggctgcgg ttctcagtcg gtctactttg ataacgggga cgttatttta
     6541 tagatacatc aagaaaaacc gcagacatcg tgcgcttcca aataaaatgg cgatacatag
     6601 tagctgtggt tttgcttgat agcatggtag tagtataagc gaggtttttt acgctgtcaa
     6661 atttttgcct gttgagactg tatacgaggg tatacaatac tatttgagaa tgaaaggcaa
     6721 taaaaagtta cttaagataa aagcaataag aaacgtcccc gtgaggctaa agacatagag
     6781 gacgaaatag gtaatccctt cttgccaagc gtcatgccac gtgttggatt ccgttctgct
     6841 cttcagcgca atgataagtg ctgcaacgag cgcaacaacg agaccaccaa taagataaaa
     6901 tcccacagaa gacatagcat aataggattg aacccctgca aatacaccaa tgagatacgc
     6961 ggagattgat ttagagtatc ccagttgaat ccataatggg tgttgttctt tttttgtcat
     7021 cgtatctccc tgcctatttg ttccactata gagggattat ctgggcgagt ctaggagata
     7081 caaagtttac ggtcgtagta tgaaaagtgg gtgggagcgt agtgctccca cccacctggt
     7141 tgagatcctg ctagggtatc cagatcctga acatgctgcc gacatagcgt actgccatga
     7201 agctggcgaa ggccaatgac atcattatgt acggcaggta tcgttgcgct gtttcgggcg
     7261 cgttgttcca gaatcgccat gcaccgaaga gcgttaatgc cattgctccg atggcaacga
     7321 tgaaccatcc tggcatggtg taaatgtatg agcagatgcc gcaacttgca ccaaatgctc
     7381 ctgcccatat cttggcgtgt tttaccgcgt ctattggcgg ttcggatttt tccgtcatga
     7441 ccctatctcc ccttattttc gtggtcagcc agatgaggaa agtatatggg atgtggccac
     7501 acttgtccag agatgaaaac tggcagcata cggaatcatc cgcatactgc cagtcttggt
     7561 tcggggtaga gcgtacctac gccgccttag cgggatgcag gtggaggttc cggaacatga
     7621 acgctcctcc ttcgggctgc gccgctgctt ccaggaacgc atcgaagtcg aatgctccga
     7681 gtccgtctcc gtggacgtag ccccggacaa tactgccctc gagcacgttt ccgttcagcg
     7741 tgacctcgac atccggctcc aggaggaacc gtcggtcgca ggttccccgg gtgcggtccg
     7801 ggagccagac ttccacgtcc tgctggcccc acgctgcgta tgcgctcgcc agcccctccg
     7861 ggtcgccttc gtaggtgaac tcatcaatcc gcgacaccgc acggtacggt gccaggcagt
     7921 tgttgtacag aaggctcgga gccccgaaca ctctgcctcg catacggaca ctgatcttcc
     7981 gctccatctg tcttcctccc ttgcgatcgt cagatattgt gtgcatccta gcacactttt
     8041 tacaaaaagt caatactcac tttgtccaga gcatggagcg gcggtactca tcgaggtgtt
     8101 caactgcaat gccggtaccg cgcacaacgc agagttctgg gtcgtcggcg acttggcacg
     8161 ggacgccagt aactttggtc atcagctcat cgatcttacg gaacttgctt gagccgcccg
     8221 tcatcacaat tcccttgtcc atgatgtcgg aggcaagttc tggcggagtt ttttggagga
     8281 caattttgag ggcgccgatg atttcgttta aggcttcctt gatggcgttc acgacgtctg
     8341 aagtagtaac taaaatactt tcgggtaagc cagaaatagc gttacttccg gaaatctcca
     8401 gggtgagatc tttgtctaag gggagcacac tcccgatttt tattttgacg tcttcggcgg
     8461 tttggtcccc aatcacgagg ttatattttt tacggatata gttggcaatg tcgtggtcga
     8521 agcggttgcc accaatacgt acgctcgtgg aggcaacaat atcccctagt gcaaccacgg
     8581 ccacttcagt ggtaccggcg ccaatgtcga cgatcatatt ccccgatggt gaggcaatcg
     8641 gaatatttgc gcccagtgcg gccgcaattg gctctttaat taaataggca tgtttggcac
     8701 cggcacctaa gcaggcgtcc atcacagcac gtcgttcggt tgaagtaatt ccagctgggg
     8761 ccgaaatcat aatatcgggt ttaaacaacc ggatacggcc ggagagctgg tttaagtaat
     8821 gtcgcagcat agcttcagta atccgaaaat tggcaatcac gccatctttt aacggatggg
     8881 cagcaataat tgagtcgggt gtgcggccga gcatttgttt ggccgctgtc ccgattgcta
     8941 cgattttatc gtcgattgtt tcaatggcca cgacacttgg ttcttgaatc acaatcccct
     9001 tttgggggac gtgaaccaaa acggttgccg tgcctaaatc aatgccaagt ctgcgtaaga
     9061 ccatgaatct actttccatt gttatgagaa attgttgcgg cgcggattaa ttttcgatcc
     9121 gctcaatttt agcgccgagg ctgttgagtc gttcgtggat gttttcgtaa ccgcgctcaa
     9181 tcaggtgcgc ctgctcaata gttgttttac cactggcgat aagtcctgcc aagacaaagg
     9241 ccataccagc acgaatgtca gggctgataa ttgtcttgcc gctaaatttg gttgggccag
     9301 taacaagggc gcgatgtggg tcgcagagca tgattgttcc acccattttt acgagttcgt
     9361 cgatatacaa aagacgacgg tcgtacatcc agtcatgaat gagtgagttg ccttcagctt
     9421 gtgtggcgag aatcgtcatt tgtggctgga ggtcggttgg gaataaaggc caaatgttaa
     9481 tttggatatt cgtgggctta tacgtctctc ggctgcggat gtggagttta ttaccctcaa
     9541 attcggttat aactccaaac tgattcagct tgtaaataat gctcgagagg ttttcttgtt
     9601 ctacgcctac gattgtgcct gatccgcgcg taatggcaaa tgcaaccgcc aatgtcccag
     9661 tttctatttc gtcggcgtta acatgtgcag tggtgccatt gagtgatttt gcgccacgta
     9721 tcgtaatcgt gtgtgtgcct aatccttcca cgtgtgcgcc catgtcgttt aacatctttc
     9781 cgaggcttac aatgtgtggc tcgcaggcgg ctggctcaat gatggttgtt ccatgagcta
     9841 gagccgcagc catcatggcg ttttcggtag cggtgaccga ggcttctcct aggtacatac
     9901 gggaaccaat cagctgagca gcttggccgt agtacttaat ttcgtcttgt tgaaacgttg
     9961 ctcctaaagc ttggagggcg tcaaagtgca caccaacagg acgtttccca aggacgcagc
    10021 cgcctgggtg acggagagtg attttgcctg cacgggcaat gatcggtcca agcagtaaaa
    10081 cggatgcgcg aagtttagac gccaggtcgt cggggatctg ggttgtcaca aggtgaggtg
    10141 tttctacgct gaccgtgttg ccatcaatct ctactgtggc gccaaggtgg cgcaggatgt
    10201 cgagcattac gtggatgtca cggatcattg gtactcggtg gagggtacat ttctcggaag
    10261 tcaaaagagt tgctgccagc attggcagtg cggcattctt tgaaccgtta acggtaatct
    10321 ctcctcgaag cttatgacca ccttcaatga cgtacttcac gctcataatc aacctcgctt
    10381 tt
//
LOCUS       DASWYB010000004        12792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_923604, whole genome shotgun sequence.
ACCESSION   DASWYB010000004 DASWYB010000000
VERSION     DASWYB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923604"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..789)
                     /gene="map"
                     /locus_tag="VGE59_00160"
     CDS             complement(22..789)
                     /gene="map"
                     /locus_tag="VGE59_00160"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY1074102.1"
                     /translation="MTIRPKTSEELDIMRESGKRLNAVLKAVNDFITVGKSLQEIDAY
                     AYDLIIKSGAKPSFLGYLDYPASTCLSVNDAVVHGIPDDYILKEGDLIGVDIGVCYEG
                     YHTDAAFTKGIGAISKEAKKLLQVTQEALGIALEATIAGNTVGDIGSAVEAYVKSQGK
                     YGIVRDLSGHGVGKNLQESPEILNYAAKNPTPLVNGMTLAIEPMINLGDWRVVVDDDD
                     WTVRTRDGMMSAHFETTVIVQDNDPEVLVDFPLDFPC"
     gene            complement(764..1318)
                     /locus_tag="VGE59_00165"
     CDS             complement(764..1318)
                     /locus_tag="VGE59_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY1074103.1"
                     /translation="MIYIFVGPPASGKSTQAKLLAKELGYPYVSVSSLLRERVAREPH
                     LREVMKEGDLLPSYIVEEALDHYFTKHPDNVVIDGAFRRGKEVNTLARIWKEAEKVAI
                     LMVTSHDIIRHRALGRNEGREDDDPTVVEHRIRLYDKTVPSIIEHVEANDIPLIRVDG
                     NQSIEAIHKNIVGKLIHDHTTKDE"
     gene            complement(1315..2019)
                     /locus_tag="VGE59_00170"
     CDS             complement(1315..2019)
                     /locus_tag="VGE59_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY1074104.1"
                     /translation="MDPRTANWIFGVITVGALAFAGITYVNKSNSQLPQPTISVVPTG
                     SSSPQTSATMSPKATATPVPSASPANSRKFTTSQGLSLPNEVETIGTGGSLSTIAGEH
                     NMTATELAQLNGIADANTIYAGQTIIIPDDIDTGTYTILYILNTTRLTKEKSKIESGA
                     SSLYSESVTAAQTDLKGIFSLKADSPFSKSNETEKNVTLSTTSEDKIFTATMEKHESG
                     LWIVKKLVVKETKATP"
     gene            complement(2028..3302)
                     /gene="secY"
                     /locus_tag="VGE59_00175"
     CDS             complement(2028..3302)
                     /gene="secY"
                     /locus_tag="VGE59_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY1074105.1"
                     /translation="MWNAIIRFWQLKDLRRKVIVTIFLLAIARTLIYIPLPLIQTSAL
                     KELFAQNAFLGFLNIFSGGGLENFSIITMGVGPYITASIIMQLMGVIIPSLEELQQEG
                     EYGQKKINQYTRLLTLPLAIIQAYSVLLLLKQNGVILDWTPVNVVVTLIMMATGTLFV
                     MWLGEIISERGIGNGISLLITLGIIGALPLHIQNMVSTAQTSGNYTMILSLLAITLLS
                     IVFVIIVNEAKRLIPIAYARRIRTGGFGSVPSYLPIKVNTAGVVPIIFAIAILTVPAF
                     LGQFLSQARSEWLAKSAQWLTTTFQPSSNPYNIVYFLLVFIFTFFYTYIIFKPEKIAE
                     NLQKQGGYIPGVRPGTETIRYLQKIVNRITLFGALFIALIAILPVLLQYQFPSQAVLL
                     SGTSLLIVISVVLETGRQIQSQYIMRTYDAYA"
     gene            complement(3453..3851)
                     /gene="rplO"
                     /locus_tag="VGE59_00180"
     CDS             complement(3453..3851)
                     /gene="rplO"
                     /locus_tag="VGE59_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006304558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY1074106.1"
                     /translation="MMMHEIQPSTPRKSGNRVGRGISAGQGKTAGRGTKGQKSRSGYN
                     IPRKFEGGQTPLILRLPKVRGFKHHRLETQLVDRSLINKHFTDGEVVSPTTLAQKGLI
                     KSLDLPVKVLGSEKVTINVTYEDVKQTKVK"
     gene            complement(3861..4466)
                     /gene="rpsE"
                     /locus_tag="VGE59_00185"
     CDS             complement(3861..4466)
                     /gene="rpsE"
                     /locus_tag="VGE59_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY1074107.1"
                     /translation="MAKTKEEHDIMNKDVEKEIAVVADVPAAPAEQVESTPLEERVVA
                     IDRVSRTQKGGRRMRFRATVVVGDRNGSVGLGIGKANEITSAVAKATYQAKKNIVTVP
                     IVRHTIPHEIIGKFDTAIVLLKPAQVGTSIIAGGAVRPILELAGVENVVSKCLSRTTN
                     KLNNAYATLKALTTLKDIKEVAPRHYVKAKKAVKPTVKATA"
     gene            complement(4490..4834)
                     /gene="rplR"
                     /locus_tag="VGE59_00190"
     CDS             complement(4490..4834)
                     /gene="rplR"
                     /locus_tag="VGE59_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY1074108.1"
                     /translation="MDKKQQLRLKRHTKIRHRISGTAERPRLSLYRGLRHLYAQLIDD
                     VAGKTILGLSDKTLVEKGTGMERAEALGKELAKRAQDKKITTIVFDRAGFRYHGQVKK
                     VADTLRDAGITV"
     gene            complement(4837..5382)
                     /gene="rplF"
                     /locus_tag="VGE59_00195"
     CDS             complement(4837..5382)
                     /gene="rplF"
                     /locus_tag="VGE59_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY1074109.1"
                     /translation="MSRIGSRLISIPSGVTANITDGIFTAQGPKGQHTVVVHPRVNVA
                     VEGETITVSRGGNDKQARALHGLMRMLVANAVTGVSQGFTKKLEMHGIGYRAQTDGNQ
                     LTLSVGFTHPVVFTAPEGISFTVEKNTLVGVHGIDKQVVGQVAANIRAVKKPEPYKGK
                     GIRYEGEYVRRKAGKAAKAGK"
     gene            complement(5408..5800)
                     /gene="rpsH"
                     /locus_tag="VGE59_00200"
     CDS             complement(5408..5800)
                     /gene="rpsH"
                     /locus_tag="VGE59_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY1074110.1"
                     /translation="MVNDPISDMFIRIKNAQAVKKTTVVMPASKLKESIAKVLVENKY
                     VASYERKSAKPCDLLELTLSYTNKEPVISHLRRVSKPGLRRYSGPAEFPRPLSGHGLV
                     IVSTTQGVMSGAQAQKAGIGGEIIATVW"
     gene            complement(5810..5995)
                     /locus_tag="VGE59_00205"
     CDS             complement(5810..5995)
                     /locus_tag="VGE59_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY1074111.1"
                     /translation="MAKKSMIVKAQRKPKFSTRIVRRCQVCGRPRGYLRAFGTCRICF
                     REMASRGEIPGITKASW"
     gene            complement(6004..6552)
                     /gene="rplE"
                     /locus_tag="VGE59_00210"
     CDS             complement(6004..6552)
                     /gene="rplE"
                     /locus_tag="VGE59_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY1074112.1"
                     /translation="MNSLREKYTQNQSAISTTLGLKNVMELPKLKKISVNIGMGELKG
                     NDALQKQVAEILSIITGQKPVQTRAKRAIAGFKLRENDVIGYRVTLRGERMYDFLDRL
                     VTYVFPRLRDFQGISSTGFDRHGNFTFGLREQTVFPEVPYQGAEKTWGMQITLVTSAT
                     DDAGARALLEAFGFPFTKSAAK"
     gene            complement(6562..6867)
                     /gene="rplX"
                     /locus_tag="VGE59_00215"
     CDS             complement(6562..6867)
                     /gene="rplX"
                     /locus_tag="VGE59_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003728539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY1074113.1"
                     /translation="MKLKKGDTVLIIAGKDRGKTGKIEAVLPTKGRVLVAGVNAFKKH
                     VKPSAQNPQGGVVEAFRAIDASNVMLLDPETQKPTRIGYQGNGKDKVRTSRLTNKAI"
     gene            complement(6921..7289)
                     /gene="rplN"
                     /locus_tag="VGE59_00220"
     CDS             complement(6921..7289)
                     /gene="rplN"
                     /locus_tag="VGE59_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY1074114.1"
                     /translation="MTQMLTQFKVADNSGARMIAQITTVKGGKIDAAGLGDTIVGSVK
                     KAIPHGNVSKKAVVKAVIVRTTQPHRRADGSVIRFDDNAVVIVNNDGKPVGTRVFGPI
                     AREIRDKGFREIVSLAPEVL"
     gene            complement(7291..7572)
                     /gene="rpsQ"
                     /locus_tag="VGE59_00225"
     CDS             complement(7291..7572)
                     /gene="rpsQ"
                     /locus_tag="VGE59_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY1074115.1"
                     /translation="MGKVRTGIVISNKMDKTAIVRVDRMVPHKKYGKRFRVSQNFAAH
                     DETNSVQIGDSVTIRETSPFSKTKNWEIVTGEMATKEAPHGAPAGEVKE"
     gene            complement(7574..7807)
                     /gene="rpmC"
                     /locus_tag="VGE59_00230"
     CDS             complement(7574..7807)
                     /gene="rpmC"
                     /locus_tag="VGE59_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY1074116.1"
                     /translation="MKRAEHIAQLGKQSLVDIKKDIHELEKKIQAHKLAISFGKAKDV
                     RTLRNYKRDLARSLTIANQKLVTMSVDTQGSEK"
     gene            complement(7809..8222)
                     /gene="rplP"
                     /locus_tag="VGE59_00235"
     CDS             complement(7809..8222)
                     /gene="rplP"
                     /locus_tag="VGE59_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY1074117.1"
                     /translation="MLAPRKVKHRKAFRGKRGGYSYRGAELSFGTYGIKALDRGWITA
                     RQIESARRAMTRYIKRGGQVWIRVFPDKPVSQLPAEVTMGGGKGAPSHFVAVVRPGRM
                     MFEMAGVSEEVAREAMRLAQHKLPIKTKFIVKEEV"
     gene            complement(8222..8884)
                     /gene="rpsC"
                     /locus_tag="VGE59_00240"
     CDS             complement(8222..8884)
                     /gene="rpsC"
                     /locus_tag="VGE59_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY1074118.1"
                     /translation="MGHKVHPKSARLLLTHDWDSKWFDIKNFATYVVEDARIRSMVAK
                     KLGPQAAIDRIVIERKRGQLTITLHAGRPGVIIGRAGAGINDLRQFVEKQVLKNSANA
                     KNVKILVSQIKIPELSAAFVAQNIGQQITRRINYRRAVKQAIEKTMQRGAKGIKVAIA
                     GRLNGAEIARSEKFIEGKLPLSQFKADIDYALYHTPTTYGVIGVKVWIYKGDMQVTEG
                     DA"
     gene            complement(8887..9270)
                     /gene="rplV"
                     /locus_tag="VGE59_00245"
     CDS             complement(8887..9270)
                     /gene="rplV"
                     /locus_tag="VGE59_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY1074119.1"
                     /translation="MSVTVKQKFIHMAPRKLRLVADSVRGKAADAAYASLTIMPRAAA
                     KPVMHAIKSAVAAAKQQDLGTSLVISAVFVDEGPALKRRIFRSRGRATRMEHRMSHLT
                     LTVTPAQAKVKPAKKAPQNTAEDKE"
     gene            complement(9283..9564)
                     /gene="rpsS"
                     /locus_tag="VGE59_00250"
     CDS             complement(9283..9564)
                     /gene="rpsS"
                     /locus_tag="VGE59_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041304992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY1074120.1"
                     /translation="MSRSLKKGPFIDERVAKKLAQLGGEKKPIKTWARDCTIFPEMVG
                     HTFLVHNGKDHLSVFVVEDMVGHKLGEFSPTRKFRRHGGKIAKEQAKGK"
     gene            complement(9618..10457)
                     /gene="rplB"
                     /locus_tag="VGE59_00255"
     CDS             complement(9618..10457)
                     /gene="rplB"
                     /locus_tag="VGE59_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY1074121.1"
                     /translation="MADIRIVRPTTSARRKMSYLTYEEVTKKTPEKSLLIHLKKHAGR
                     DRNGHISIRHRGGGAKRMYRMVDFSGLKHFGQTATVLAVEYDPNRSAFIALIQYEGGK
                     KAYMIAPQGIEVGQTITTDKTASLTTGNRLPLSEIPTGTPIYNIELTPGKGGQLVRSA
                     GTTATILAKEGKYANVRLPSGEVRMIHVTCMATIGEVSNKAHNTIRIAKAGRTRHMGR
                     RPTVRGKAMNPVDHPHGGGEGHTSIGLKHPKTPQGKPAMGYRTRGRKTSSKFIVRRRG
                     KKR"
     gene            complement(10460..10840)
                     /locus_tag="VGE59_00260"
     CDS             complement(10460..10840)
                     /locus_tag="VGE59_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY1074122.1"
                     /translation="MKTFFVQPLITEKSMAKTADGVYQFVVPTWATKRQIAEFIGRTF
                     NVTVVNVTTALLRGENVRFRQRLGKQSTYKKATVRLVKGDTIGDFSLPVESETAPMGK
                     TEADAVTESKITVRNKKTAQKAGA"
     gene            complement(10842..11504)
                     /gene="rplD"
                     /locus_tag="VGE59_00265"
     CDS             complement(10842..11504)
                     /gene="rplD"
                     /locus_tag="VGE59_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY1074123.1"
                     /translation="MAQLTLPIFDLTGKETSTIELNAAIFGVAANPTLLHQAVVAFLA
                     NKRAATASTKDRSQVSGTNRKPWKQKGTGNARVGSARSPLWRGGGITFGPNADRNYGQ
                     RMPQKMKQSALKVALSSKVAGDGFIVVDSLDALDGKTKTWITAASKLPKAESKKLVVG
                     LAKNDMADRSIRNVQGVKYVSLEGLTIYDVVRYPVVMMTQDAIASLEKRLTNRAKEAV
                     KA"
     gene            complement(11506..12126)
                     /gene="rplC"
                     /locus_tag="VGE59_00270"
     CDS             complement(11506..12126)
                     /gene="rplC"
                     /locus_tag="VGE59_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY1074124.1"
                     /translation="MVTAVFAEKLGMTHVYNDKNRHIPVTILRVAPQVVTALRTQEKD
                     GYAAIQVGATGRKHVAKPQAEELKKSNIELPISHRKEIRIEGEADMTVGQEMNVTIFA
                     AGDKVMITGISKGKGFAGTIKRHNFSRGPETHGSKNVRKPGSIGGGYPQRVVLGKRMS
                     GHMGHETVTTKGHKVVAINEKDNTIAISGAVPGPNKGRVFIQKVEK"
     gene            complement(12456..>12792)
                     /locus_tag="VGE59_00275"
     CDS             complement(12456..>12792)
                     /locus_tag="VGE59_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY1074125.1"
                     /translation="ARAVVKNPTVLVFDEATSSLDSKSERAILQTLDSVSKGRTTISI
                     AHRLSTIVDSDTIFVLQKGKVVEQGRHTELLQKNGVYAHLWELQARTHDENGSVEEKA
                     TSHETSAPS"
BASE COUNT         3200 a         2872 c         3011 g         3709 t
ORIGIN      
        1 taacatactt ccacaggagc gtcaacaggg aaaatcaaga ggaaagtcga caagtacttc
       61 gggatcatta tcctgcacaa tgacagttgt ttcgaagtgg gcactcatca tgccatcacg
      121 tgtacgaact gtccaatcat cgtcatcaac gacaactcgc cagtcgccta agttgatcat
      181 cggctctatg gcgagggtca tgccattcac aagaggcgtt gggttttttg ccgcataatt
      241 tagtatttct ggagattctt gaagattttt accgacgcca tggccagata aatcacgtac
      301 aatcccatat tttccttggg atttcacata tgcttccacg gcgctaccaa tgtcgccgac
      361 tgtatttccg gcaattgttg cttctaatgc gatgcccaag gcctcttgtg ttacctggag
      421 aagctttttt gcctctttgg aaatagcgcc aattcctttc gtaaaagcag cgtctgtatg
      481 gtatccctca taacatacac caatgtcgac accaatcagg tcaccttctt taaggatgta
      541 atcgtccggg ataccgtgca cgactgcgtc attaaccgat aggcaggttg aagcaggata
      601 atctaaatac cctaaaaatg atggttttgc tccgcttttt ataattaagt catacgcgta
      661 ggcatcgatt tcttggagag attttccaac tgtaataaaa tcattgacgg ctttgagaac
      721 ggcgtttagc cgtttgccgc tttcgcgcat gatgtcaagt tcttcactcg tctttggtcg
      781 tatggtcatg aataagtttt cctacaatat ttttatgaat agcttctatg ctttgattgc
      841 catcaacacg gataagcgga atatcgtttg cttcaacatg ctcaatgatt gaggggacgg
      901 ttttgtcata taagcgaatt ctatgttcaa cgacggtcgg atcgtcatct tcccgccctt
      961 catttcttcc caacgcgcga tggcgaatga tgtcatggga agtaaccatg agaatagcaa
     1021 ccttttctgc ttccttccat atccttgcta acgtgttaac ttctttgcct cgtcggaatg
     1081 ccccgtcgat gacaacgttg tcgggatgtt tggtgaaata atggtccagt gcttcttcga
     1141 caatatacga cggcagcaga tccccttctt tcattacttc acggagatgc ggctcacgtg
     1201 caactctttc gcgtagcaga gagctaacac ttacgtaagg ataaccaagt tctttggcta
     1261 agagttttgc ctgggtgctt ttacccgacg caggaggtcc gacgaaaatg taaatcacgg
     1321 agtcgccttt gtttctttta ctacaagctt tttaactatc cataaaccac tttcatgttt
     1381 ttccatcgtc gcggtaaaaa tcttgtcttc agaggtagta gaaagggtaa cattcttttc
     1441 ggtttcattt gacttactaa aaggagagtc ggcttttaag ctaaaaattc ctttaaggtc
     1501 agtttgtgcc gctgtcacac tctcgctgta aagactgctt gctccagatt cgattttgct
     1561 tttctctttt gtgagacgcg tggtattaag gatatacaag atagtatagg tacctgtatc
     1621 gatatcgtcg ggaattataa tggtttggcc agcataaata gtatttgcat cggcaatacc
     1681 gttgagctgg gcgagttctg ttgctgtcat attgtgctcg ccagcaatgg ttgaaagact
     1741 gccgcccgta cctattgttt caacttcatt agggagagaa agcccttggc tcgtagtaaa
     1801 tttacgtgag tttgcaggag aggctgaggg aaccggcgtg gcggtcgctt ttggagacat
     1861 tgttgcgctt gtctgaggag aacttgatcc tgtgggtacg actgaaatag ttggctgcgg
     1921 taactgagag ttcgatttat tgacataggt aattccggca aaagccagag ctcctactgt
     1981 gataacaccg aaaatccaat tagcagttcg aggatccatc gatttcctta agcatacgca
     2041 tcataggtgc gcatgatgta ctgggactga atttggcgac ccgtttcgag aacgacagaa
     2101 ataacgatga gcaagcttgt accacttaag aggacggctt gtgaagggaa ttgatattgc
     2161 aacagcacag gtaagatggc gatcaaagcg ataaacaaag cgccgaataa tgtgatccgg
     2221 tttacgattt tctgtaagta gcgaattgtc tcggtacctg ggcgcactcc agggatatag
     2281 ccgccttgct tttgcaagtt ttcagcaatt ttttcaggct taaagataat ataggtgtag
     2341 aagaatgtga agataaagac taagagaaag tacacaatgt tgtaagggtt gctggaaggt
     2401 tggaaggtgg tggtaagcca ctgtgctgac ttggcgagcc attcgcttct tgcttgggat
     2461 aggaactgac ctaagaaggc aggtaccgtt aatattgcga tagcaaagat aatgggcaca
     2521 acgccagcag tatttacttt aataggaagg tagcttggca cactgccaaa tccgcctgtg
     2581 cgaatacgcc gagcgtaggc tatcgggatc aatcgtttgg cttcgttcac aataataaca
     2641 aagacaatcg aaagaagtgt gattgccaga agcgaaagaa tcatagtgta gttaccactt
     2701 gtctgagctg tgcttaccat gttttggatg tggagtggta atgcgccaat aatacctaaa
     2761 gtaatcaaaa gcgaaatacc attgccaatt cctcgttcac tgataatttc gccaagccac
     2821 ataacaaaga gtgtaccggt tgccatcatg atgagagtta cgacaacgtt tacaggtgtc
     2881 caatcaagaa taacaccatt ctgtttgagg agcagtaaaa cagaataggc ctgaataatt
     2941 gcgagaggaa gtgttaaaag gcgggtgtat tggttgattt tcttttgtcc atactctcct
     3001 tcttgctgga gctcttctaa cgaggggatg ataacgccca ttaactgcat aatgattgag
     3061 gcggtaatgt atggcccaac acccattgtg ataatcgaaa aattttctag tcccccgccc
     3121 gagaaaatat ttaaaaagcc aagaaaggcg ttttgcgcaa aaagttcttt gagagcactc
     3181 gtctgaatga ggggtaaagg aatgtaaatg agtgttcggg cgattgccaa taagaagatc
     3241 gtaacgatca ctttgcgacg taaatcttta agctgccaaa atcgaattat cgcgttccac
     3301 atatactgct attcctttat ttgcaatgac aaaaaaactt ttcaataatg aggttattgt
     3361 accccgagta agggcaaatg gcaaatgaag gatacctggc aagatactaa aagccgccag
     3421 tcaggcggct tttagtatct tgggtaaatc gcttacttta cctttgtttg tttgacgtct
     3481 tcatatgtta cgttgatcgt aactttttca gatccaagga cttttacagg gagatctaaa
     3541 gatttgatca agcctttttg agcgagcgtc gtgggagata ctacttctcc gtcggtgaaa
     3601 tgcttgttaa taagagaccg atcgacgagt tgtgtttcaa ggcggtggtg tttaaagcca
     3661 cgcactttgg gtaaacgaag aataagtggg gtctgtccac cttcaaattt acgtgggata
     3721 ttgtatcccg aacgtgattt ttgccccttg gttccacggc cggcggtttt accttggcca
     3781 gcagaaatac cgcgtccaac gcgattacca ctcttgcgcg gtgttgatgg ttgaatttcg
     3841 tgcatcatca taacgagtcc ttatgccgtt gcttttactg tcggcttaac agcttttttc
     3901 gctttgacgt agtgacgagg agccacttct ttaatgtctt ttaacgttgt taaagctttc
     3961 agtgtagcgt aggcgttatt taatttgtta gttgtacggg aaagacactt actgacaacg
     4021 ttctctactc ctgccagttc taaaatggga cgaactgcgc ctccagcaat aatagaagta
     4081 cctacttggg ctggtttaag aagaacgatc gctgtatcaa atttaccgat aatttcgtga
     4141 ggaatagtat ggcgaacaat aggaacagtt acgatgtttt tcttcgcttg gtacgttgcc
     4201 tttgcaacgg ccgaagtgat ttcgttagct tttccaatac ctaaaccaac gctaccatta
     4261 cggtctccaa ccacaacagt ggcacggaaa cgcatacggc ggccaccttt ttgggtacgg
     4321 cttacacgat caatagcgac cacccgttct tctagtgggg ttgattcgac ttgctcagca
     4381 ggagcagccg gtacgtcggc aacaacagca atttcttttt cgacgtcttt attcatgata
     4441 tcgtgttctt ccttggtttt cgccataaca attttctcct ctgaggggat tagaccgtaa
     4501 ttcccgcgtc acgtaaagta tcagcaacct tttttacttg gccatgataa cggaatcccg
     4561 cgcggtcaaa aacaatagta gtaattttct tatcttgggc acgtttggca agttcttttc
     4621 caagtgcttc ggcccgttcc ataccagttc ctttttcaac gagtgtctta tcggaaagtc
     4681 caagtatagt cttgccggcg acatcgtcga taagttgagc ataaaggtgg cggagcccac
     4741 gataaagact taaacggggg cgctcggccg taccgctaat acggtgacga attttagtat
     4801 gacgcttgag gcgtaattgc tgtttcttat ccatgattat ttccctgctt ttgcggcttt
     4861 accagcttta cggcgcacgt attcaccttc gtagcgaata cctttacctt tgtatggctc
     4921 aggttttttg acggcgcgga tattagcagc cacctggccg acaacttgct tatcgatgcc
     4981 gtgaacacca accagagtat tcttttcaac ggtgaaagaa atgccttctg gggcagtaaa
     5041 gacaactggg tgtgtaaaac caacacttaa agtcagttgg tttccgtcgg tttgagcgcg
     5101 gtatccaata ccgtgcattt caagtttttt ggtgaagcct tggctcacac cagtaactgc
     5161 gttggcgacc aacatacgca ttaatccatg aagggcgcgg gcttgcttat cattgccacc
     5221 acggctaacg gtaatggttt caccttcaac agcaacattt acgcgagggt gtactacaac
     5281 agtatgttgt ccttttggac cctgtgcagt aaaaatgccg tcggtaatgt ttgccgtgac
     5341 accagatggg attgagatta atcgtgagcc aattcgtgac atatgaatcc tttacttcga
     5401 ccgacaatta ccaaacggtt gcgataattt cgccaccgat tcccgctttt tgagcttgtg
     5461 caccgctcat cacaccttgg gtggttgaga cgataacaag tccatggcca cttaatgggc
     5521 gtgggaattc agcaggacca ctgtaacgtc gtaatccagg tttggatact cggcgtaggt
     5581 ggctaataac aggttcttta tttgtgtagc ttaatgtaag ttcgaggagg tcgcatggtt
     5641 ttgcgctctt tcgttcatag ctggcaacat atttgttttc aaccagaact ttcgcaatgc
     5701 tttcctttaa tttggaagca ggcataacca cagtcgtctt tttgacggcc tgggcgttct
     5761 tgatgcggat aaacatatct gaaattgggt cgttaaccat aacttactcc taccaagagg
     5821 cctttgtaat acctgggatt tcgccacgtg aggccatttc gcggaaacaa atacggcacg
     5881 taccaaaggc acggaggtat ccgcgtgggc gaccacaaac ctggcagcga cgtacaatac
     5941 gagtgctgaa tttaggcttg cgctgggctt taacaatcat acttttttta gccatatcaa
     6001 ctcctattta gccgcacttt tggtgaatgg gaacccaaag gcttcaagca gtgctcgagc
     6061 gcctgcgtca tcagttgcac tggtcacgag ggtgatctgc ataccccatg ttttttctgc
     6121 tccctggtaa gggacttcag gaaaaactgt ttgttcacgt aatccaaagg tgaagttacc
     6181 atgtcggtca aatccggtcg atgaaatacc ttggaagtca cgtaagcgag gaaaaacata
     6241 ggtgactaaa cgatcaagaa aatcatacat gcgctcaccg cgaagagtta cacggtatcc
     6301 aatcacatcg ttttcgcgaa gtttaaaacc ggcaatagca cgtttcgcac gagtttgtac
     6361 tggtttttgg ccggtaataa tactgaggat ttcagcaact tgcttttgga gcgcatcgtt
     6421 gcctttaagc tcacccatac caatattaac gctaattttt ttcagcttag gtaattccat
     6481 gacgttttta aggccaaggg tcgtgctaat agcggattgg ttctgagtgt atttttcgcg
     6541 taagctattc ataatctaac cttaaattgc tttattcgta agacgcgagg tacggacttt
     6601 gtccttaccg ttgccctgat acccaatacg ggttggtttt tgagtttctg gatcgaggag
     6661 cattacattg ctcgcatcaa tagcacggaa agcttcgact actccgcctt gagggttctg
     6721 ggcgcttggt ttgacgtgct ttttgaaagc attcacaccg gcgacgagaa cgcgaccctt
     6781 ggttggtaag acagcttcaa tcttaccggt cttaccccgg tctttgcctg caatgattaa
     6841 aactgtatca ccttttttga gtttcatagt ttctccgtgg actctgctag tatacctgtt
     6901 tttaccttat ctgtcgagtg ttaaagcact tcgggcgcga gcgagacgat ttctcggaag
     6961 cctttatcac gaatttctcg agcaattgga ccaaatacgc gggttccaac tggttttccg
     7021 tcgttattga cgattacaac tgcgttatca tcgaaacgga taacgctgcc gtcagcacgt
     7081 cggtgtggct gagtggtgcg aacaataaca gcttttacca ctgctttttt cgagacgtta
     7141 ccgtgaggaa tagctttctt tacgctgcca acaattgtgt cgccaaggcc tgctgcatcg
     7201 attttcccac ccttaacagt cgtgatctga gcgatcatgc gtgcgccgct gttatcggca
     7261 actttgaatt gagtcagcat ctgtgtcatg ttattccttt acctcgccag caggcgctcc
     7321 gtgaggagct tcctttgtgg ccatttcgcc cgttacaatt tcccagtttt tagttttgct
     7381 aaatggactt gtttcgcgaa ttgtgacgga atcgccaatt tgaacgctat tcgtctcgtc
     7441 gtgagccgca aaattttggg atacacggaa acgtttgcca tatttcttgt gtgggaccat
     7501 tcggtcaaca cgaacaatgg cagttttatc catcttattt gagataacaa tacctgttct
     7561 aaccttaccc attttacttc tccgaacctt gagtgtcaac gctcatggtg actaactttt
     7621 ggttagcaat tgtgaggcta cgagcgaggt cacgtttgta attacgtagt gttcgcacat
     7681 ctttggcttt gccaaaagaa atggcgagct tatgtgcttg aattttcttt tcgagctcat
     7741 ggatatcttt tttgatatca acgaggcttt gtttgccgag ctgagcaatg tgttctgcac
     7801 gcttcatatt atacctcttc cttcacgata aactttgttt taataggaag tttatgttgt
     7861 gccaaacgca tagcttcgcg agcaacttct tcgcttactc cagccatttc aaacatcatg
     7921 cgtccgggac ggacaacagc gacaaagtga cttggagcac cttttcctcc acccatcgta
     7981 acttcagcgg gaagctgcga aactggtttg tcggggaaaa cgcggatcca tacttggcca
     8041 ccacgtttaa tataacgggt catagcacgt cgggcagatt cgatctgacg agcagtaatc
     8101 catccgcgat cgagggcttt aataccgtac gtaccaaagg aaagttcggc accacgatat
     8161 gaataaccac cgcgtttgcc acggaaggcc ttacgatgtt taactttgcg tggcgctaac
     8221 attatgcatc tccttcagta acttgcatgt cacctttgta aatccaaacc tttacaccaa
     8281 taaccccata ggtggttggc gtgtgataga gagcgtagtc aatatcagct ttgaattgtg
     8341 aaagaggtaa cttgccttca ataaactttt cgcttcgagc aatttcagct ccgtttaaac
     8401 gaccagcaat ggccacttta atacctttgg cacctcgttg cattgtcttt tcaatggctt
     8461 gcttaacagc gcgtcgatag ttaatacgac gggtgatttg ctgaccaatg ttctgggcga
     8521 caaacgcagc ggaaagttca gggattttaa tttgtgaaac aaggatctta acgtttttag
     8581 cgttagcaga gtttttgaga acttgctttt cgacaaactg acgaaggtcg ttaataccag
     8641 cacctgcacg accaataatc acaccagggc ggcctgcgtg taacgtaatg gttaactgac
     8701 cacgcttacg ttcaataacg atgcggtcga tagcagcctg tggaccaagc ttttttgcaa
     8761 ccattgaacg gatacgagca tcctcaacca catacgtagc gaagttttta atgtcaaacc
     8821 acttgctatc ccaatcatgg gtaaggagga ggcgtgcgct ttttggatgt actttatgtc
     8881 ccataactac tccttatcct cggcggtatt ctgaggggcc ttttttgccg gctttacttt
     8941 tgcctgtgca ggggtaacgg tcaatgttaa atggctcata cggtgttcca tgcgggtggc
     9001 gcggccacgt gaacggaaaa tacgacgttt aagagcaggg ccctcgtcaa caaaaacagc
     9061 actaataaca aggcttgtgc ctaagtcttg ctgcttcgca gcagcaactg ctgatttaat
     9121 ggcatgcata actggcttgg ccgctgctcg gggcataatc gtgagcgagg catacgcagc
     9181 atcagcagct ttcccgcgga ctgaatccgc aactaagcgg agcttccgtg gggccatgtg
     9241 gatgaatttc tgttttacag ttacactcat ggtctttcct tattacttac ctttggcctg
     9301 ctctttggcg atcttaccac catgacggcg gaatttacgg gttggtgaaa attcaccaag
     9361 cttgtgacca accatgtctt cgacaacgaa gacggaaagg tgatctttgc cgttatgaac
     9421 gaggaatgtg tgtccaacca tttccgggaa aatggtgcaa tcgcgagccc aagtcttgat
     9481 tggtttcttc tcaccaccaa gctgagccaa ctttttggca actcgctcgt cgatgaaggg
     9541 tccttttttc agtgaacggc tcatgtatac tttctcctgt acgaattata ccttagataa
     9601 ccttaattag ctagcttcta gcgcttcttg ccgcgtcggc ggacaataaa cttgcttgaa
     9661 gtcttgcgtc cacgggtacg ataacccatg gctggtttac cttgtggggt ctttggatgt
     9721 ttcaaaccaa tcgaggtatg accttctcca ccaccgtgag ggtgatcgac agggttcata
     9781 gctttaccgc gaacggttgg acggcggccc atgtgacggg tacggcctgc tttagcaata
     9841 cggattgtgt tatgggcttt gttagaaact tcaccgatag ttgccatgca agttacgtga
     9901 atcatgcgga cttcaccact tgggagacga acgttggcgt acttaccttc tttggcaaga
     9961 atggttgctg tagtaccagc agaacgaacg agctgaccgc ctttgcccgg agtaagctca
    10021 atattgtaaa ttggcgtacc agtagggatc tcggaaagcg gtaaacggtt tccggtcgtt
    10081 aacgatgcgg ttttatcggt tgtaatagtc tggcccactt caatgccttg tggggcaatc
    10141 atgtacgctt ttttgccgcc ttcatactga atgagggcaa taaaggcgct acggttggga
    10201 tcgtattcaa cggccaatac tgtggcagtc tgcccaaagt gtttaagtcc agaaaagtca
    10261 accatacggt acatccgttt ggcaccacca ccacggtggc gaatcgagat gtgaccattg
    10321 cggtcgcgtc cggcatgttt tttgaggtgg atcaggagtg atttttcagg cgtcttcttt
    10381 gtcacttcct cataggtgag gtaggacatt ttacggcgcg cagaagtggt tggtcgaaca
    10441 atgcggatat cagccataat tatgctccag ccttctgagc cgtcttctta ttgcggacgg
    10501 taatcttgct ttcagttact gcatcagctt cggtttttcc cattggtgca gtctctgatt
    10561 ctacgggcaa actaaagtcg ccaatagtat cacctttaac caggcgaacc gtcgcttttt
    10621 tataagtcga ttgcttgcct aaacgctgac ggaaacgaac gttttcacca cggagtaacg
    10681 cggtcgttac atttacgaca gttacgttaa aagtgcgacc aatgaattca gcaatttgac
    10741 gcttggtagc ccaagttggg actacaaatt ggtagacacc gtcagcggtc ttggccattg
    10801 atttttcggt aataaggggt tgaacaaaga aagttttcat attacgcctt tactgcctct
    10861 ttagcgcggt ttgttaaacg cttttcgaga gaagcgatag cgtcctgggt catcataaca
    10921 acggggtagc ggacgacatc gtaaatagtt aatccttcga ggctcacgta ctttacgcct
    10981 tggacgttac ggatagagcg gtcagccatg tcatttttgg cgaggccaac aacaagtttc
    11041 tttgattcgg cttttggtaa tttgctcgca gcggtaatcc aggtttttgt cttgccatca
    11101 agagcatcca gtgaatcaac cacaatgaat ccatcaccag caactttgct cgaaagggca
    11161 accttaaggg cgctctgctt cattttttgt ggcatacgct gtccgtagtt gcgatcagcg
    11221 tttggaccaa aggtgatacc accaccacgc caaagtggtg aacgggcaga accaacacga
    11281 gcattacctg tacctttttg cttccaaggc ttacggtttg tacccgatac ctgggaacgg
    11341 tctttggtgc tagcggttgc ggcgcgtttg ttggctaaga aagcaacgac tgcttggtgt
    11401 aataatgttg ggtttgcggc aacaccgaat atagcagcgt tgagttcgat cgtgctggtt
    11461 tctttaccgg taagatcaaa aatggggagg gttaactgag ccatattact tctctacttt
    11521 ctggatgaac acacgacctt tgtttggccc tggaacagca ccggaaatgg cgatcgtatt
    11581 gtccttttcg ttgatagcaa cgactttatg acctttggtc gtaacggttt cgtgacccat
    11641 gtggccagac atacgcttac caagtacaac gcgctgtgga tatccaccac cgatggaacc
    11701 gggcttgcgg acgtttttcg aaccatgagt ttctggaccg cgactaaagt tgtgacgttt
    11761 aatggtgccg gcaaaacctt tacctttgct aataccagtg atcatcacct tgtcgcctgc
    11821 ggcgaaaata gttacattca tctcttgacc aacggtcatg tcagcttcgc cttcgatacg
    11881 aatttcttta cggtgcgaaa taggcagctc aatgttgctc tttttaagct cttcagcttg
    11941 aggtttggca acatgtttgc gtcctgtcgc accaacctga atggcagcgt agccatcttt
    12001 ttcttgggta cgaagagcag ttactacctg aggagcaacg cgtaaaatcg tcacagggat
    12061 gtggcgattc ttatcattat aaacgtgggt catgcccaat ttttcagcga agactgctgt
    12121 aaccatataa ctcctatagt catggcagag tgccagagga ctcatttgaa ttccactaga
    12181 aggaggtttg taattcagac tgagaaagtt agcggagatt ttgaggcgaa agccaggatc
    12241 tcgtgaggag acatagccaa agctatgtcg acgaacgaat cgtggctaga agcccaacat
    12301 attcgttaaa tttccaggat gaattataaa tctgcttctt aacatagtgg cataagaaat
    12361 tggcttagaa cttgtccggc atgccggatg ttctccaagc cattactttc tatgactcac
    12421 ttatgatact acatatattc gaagtcgtca acagattagg agggggctga agtctcatga
    12481 gacgtagctt tctcctcgac gcttccattt tcatcgtgtg ttcgagcttg gagctcccac
    12541 agatgggcgt aaacgccgtt tttctgcaat aactcggtgt gacgaccttg ttcgaccact
    12601 ttgccttttt ggagaacaaa gatagtatcg ctatcaacaa ttgtggacaa gcgatgcgca
    12661 atcgaaatgg ttgttctccc tttagaaacc gagtccagcg tttggaggat tgctcgttcg
    12721 ctcttcgagt caaggctgct tgttgcttca tcaaagacca aaacagttgg atttttgaca
    12781 actgcccgag cg
//
LOCUS       DASWYB010000005        12091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_668903, whole genome shotgun sequence.
ACCESSION   DASWYB010000005 DASWYB010000000
VERSION     DASWYB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668903"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VGE59_00280"
     CDS             complement(<1..693)
                     /locus_tag="VGE59_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEY1074126.1"
                     /translation="MTDPIELFVDQFSQMGLYPDFHTIEGGVNEPVCKVNGEDYLMFC
                     SNNYLGMTQHPAVKEAAKQAIDKYGVGPGGSRVISGNIDIIEDLEKAIAELTGTEDCL
                     TFPTGYMANAAVFRAVMDPLFNNMPVLAENSVIFSDEYNHGSIVDGCRLSKAKKVVFK
                     HDDLADLEQKIKENDLPNKLIVTESVFSLDGEIINIPAYVELAKRTGCKLMVDDAHGI
                     GVLGKHGGGVTEH"
     gene            complement(690..2147)
                     /locus_tag="VGE59_00285"
     CDS             complement(690..2147)
                     /locus_tag="VGE59_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1074127.1"
                     /translation="MAEWNKLAYKPIKEWKDIQLTNLRYVSRFLLPFQPFVQNHPEKS
                     KYLSVRSLKDFQKLPFTSKLDLLPTETNPKRSRDFILQPSIELIRKAWPKSAVLSLGL
                     KKLFGTDVKAELSHEFKPVHIHFTTGRSTSQIPFLYTAKDLETLRESGGRVFETIGAT
                     SDDIAINAFPFAPHLAFWLAYNTTTSKNMMTLQTGGGKILGTRKIMDAIENLQATILI
                     IMPGYGYHLLREAVAEKRNFSSLRIIVFGGERVSPGLRRKVKSLLNKLGAKHTKVLST
                     YALTEAKTAWVQCHEESGYHLYPDLEMIELVDRHGNPVKEGSSGEIVYTSLGWRGSVV
                     TRYRTGDFCKKMIITAPCDHCGRTTPRLHSSIERRSDMVELNLTKVKGELVNLNALHS
                     IMHEIPSIEEWQAEVRKKNDDPYEIDELILNIAFTDEKVRENDMALLQSLLQRDMGIS
                     ATINSKPLKEVIQLLGLETELKEKRIVDRRKEIRK"
     gene            complement(2185..2964)
                     /locus_tag="VGE59_00290"
     CDS             complement(2185..2964)
                     /locus_tag="VGE59_00290"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1074128.1"
                     /translation="MTHETKLHSTLEYLHPDAKTGVLLLHGFSSTPGVFSKLTRLLEK
                     QGLSYYAPLLPGHGTTPDDLALYDTEDWIKSADEAYKHLKKYCNNVIILGVSMGGNLA
                     CILANKRKVDGLILAGTPRWLQRGTWIVFYMFVLRFLGIKYYHASFKKELKDGLLIGG
                     PAASYGSLQLKSISDALHLIQDETPFYLKNIKSPTLIIQSDNDGLVNSKSGNYFFQNI
                     PAIDKQLVWISQPHHSLHKDDSVYTYILDFFNRIKNSENAS"
     gene            3072..3473
                     /locus_tag="VGE59_00295"
     CDS             3072..3473
                     /locus_tag="VGE59_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1074129.1"
                     /translation="MGEEGTIGKSVLIVDDDMTLREMYEEYLKASGYLVVTAQDGEEG
                     LAQAKQSKPTAILLDLMMPKMNGIEVLRRLKADEELKEIPVIVFTALIQDLEKQESFA
                     AGASDYVVKTEVTPKSILTKIQELTTSKGEN"
     gene            3488..3748
                     /locus_tag="VGE59_00300"
     CDS             3488..3748
                     /locus_tag="VGE59_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074130.1"
                     /translation="MNDNTPSDRGILRPRRLVIAVLVVLFIGLIVFVSQSIFISADVL
                     TVPDYNPAELKQRYQTTISASPSVTASGSPLAAVSPSPLVTP"
     gene            complement(3775..5526)
                     /gene="lepA"
                     /locus_tag="VGE59_00305"
     CDS             complement(3775..5526)
                     /gene="lepA"
                     /locus_tag="VGE59_00305"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY1074131.1"
                     /translation="MQQVRNFCIIAHIDHGKSTLADRFLEYTGTIEKNKIREQMLDSM
                     DLERERGITIKLQPVHMKYHLDGVDYELNLIDTPGHVDFGYEVSRSLAAVEGAILLVD
                     STQGIQAQTLANLAVAQKHNLTIVPVVNKIDLPAAEPEKVAFELADLLKCDPDSVIPA
                     SGKSGLGLDEILKRVVHAIPQPQQEINAPLRALVFDSQFNSYRGVIAHVRIMEGSIKA
                     GENVVMMANGKTFTVEEVGVMTIDHKPVEKLEAGDIGYIVTGFKRVADCRVGDTITHV
                     SGRATAPLEGYRQPQPMVFASLFHSDGDTTALRTALEKLSANDASITFEPHYSDAFGS
                     GFRVGFLGLLHLEIIKERLEREYNQDLVITTPVVAHTGDAMNGYEEPWVKAEIITQPA
                     YIGGIMELAESHRGIYLSLQYIGEHLEDKNTTAILEYEFPLSEIIINFSDQLKSVSAG
                     YASFSYDLIGYRAGKLVEMNILIAGDKVAPFTQIVPVSQAEARGRELTKRLKELIPKQ
                     MFEISIQATIGARVVARETVSAMRKDVTSKLYGGDVTRKNKLLDKQKEGKKKMKQMGK
                     VSLPTNVFLEALKPKSY"
     gene            complement(5604..6554)
                     /locus_tag="VGE59_00310"
     CDS             complement(5604..6554)
                     /locus_tag="VGE59_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074132.1"
                     /translation="MNKQVIFLSSLIVIILILVTISFTALVKQPSADTLTATTSSPVP
                     NQHTAYEDCLAGDPGRASWCQLLLDRGLLSSPSPTVTVTPTPLVNASPTFSGYDGYYE
                     DCISAGFSASDCAGLPHKFTTPTPTPSPTPSIDLTNYDGTYQDCIAAGFSASQCEHLL
                     KSTTPTPFVSSVTPTPLPSPTPSNNFTNYDGTYEDCIAAGFPASQCDKLPHSTPSPAL
                     SYFESTLANCTGGMTSPPAKPGPMPTPPAAFNYDINAYPDINAYYAALTAYTQSLNSY
                     NTAMTAWQQLSNANMVWQDCITEWNYEHPDAIYLSDGFHL"
     gene            complement(6564..6983)
                     /locus_tag="VGE59_00315"
     CDS             complement(6564..6983)
                     /locus_tag="VGE59_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074133.1"
                     /translation="MSWISRHKKLIYLLAALILFLLFSIALNSYLLETQNKSTSALPP
                     VPELAIPQNVDTKILNSVAVKTCLAPTIAQHPAPLPSVPSSSAATSETAAYVSRVRAN
                     YLEGINWMQDLHTSTQKCYNQQISQQYSDGAPTKVWK"
     gene            complement(7043..8722)
                     /gene="murJ"
                     /locus_tag="VGE59_00320"
     CDS             complement(7043..8722)
                     /gene="murJ"
                     /locus_tag="VGE59_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY1074134.1"
                     /translation="MVSGLKRLWHSEQDLRGAASLLVITMLLSNILGFLRDLILANAI
                     PLHILDTYYAAFRIPDFLFNLFILGAISSAFIPIFLDIKTKENDKAAWELSNNLITTS
                     LIFLGILCVGLYIFMPNLLHFFVPGFSTTDTLQAESGYIISSYAMTVKLARILLFSPI
                     FFAISYVFGGMLNANKRFFSYALAPLVYNASIIVGGLLAPGFTVTIVAWMVVVGALLH
                     ALVQLPALRKLGYKYHFVLNLKDASLRRIIRLMIPRSLSLGMSQIILLAFTRIGSLLP
                     AGAISIYNLTNNFQTTPTAIFAASIGTAVFPMLGAAVSEKDNEKYKSLLTHALKGMFF
                     FMIPSMILLWILRAHIIRLYLALNHQTWEDTIRAINTFSWFILALASQGFNLIMIRAF
                     YAHQDTKRPMYVSLFSGAVAVILAWILANRIGDVWALSMAFALAAILESLILFTLFQS
                     TYRKQLNLRSLLDTITMTLLISLASGLVARVTLSIVSDGVFVGTPGLGTTRIIPLFIN
                     LLSAALTGGLTYLAISLLLKREELQWITPKRAVKNIPLPSSEDTAQNEGLA"
     gene            complement(8769..10613)
                     /gene="ftsH"
                     /locus_tag="VGE59_00325"
     CDS             complement(8769..10613)
                     /gene="ftsH"
                     /locus_tag="VGE59_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY1074135.1"
                     /translation="MKNLLYFLGIIIFASILYTFFGNPNRPKVETVNLTRISEVIKEE
                     KVEKVTVEGNSIKAKLKDGSILRADKEPGISITDYGITPENATLEVDNNSVASFWLSL
                     IPIILPVLLLGGFIFFMYRNAQGGNMKAMSFAKTTARMVSGTQKVTFNDVAGLKNAKQ
                     ELVEVVDFLKHPEKFQKLGAEIPKGVLLVGPAGVGKTLLAKAVSGEAGVPFLTLSASE
                     FVEMFVGVGAARVRDLFAKAKKMAPVVIFIDELDAIGRQRGVGMGGSHDEREQTLNQI
                     LVEMDGFETDSRVIILAATNRPDVLDPALLRPGRFDRKVVLDLPDRSEREEILKVHVR
                     NKPVAKNIDLAVIARQTPGASGSDLKNIVNEAAILAARENKKEVGQQYFTQAIEKVLI
                     GPERKSHLMNTQERSVAAYHEAGHAIVGKNVPNGDPIHKISLVSRGMALGYTWSLPEE
                     ESRMSSKSHFEDEIAQLLAGRAAEDIIFKEVTTGASNDFERATAIARDMVMMYGMSKL
                     GHVVWGERHDSFLGRGAEVRNYSEKQAELIDATVKEIIDEQMARAKKILTEQSALLEA
                     TAQRLLKIETIEGEEFDAMYDEFNGGTKRTVKSEKTAKRTPKSKNADA"
     gene            11011..11391
                     /locus_tag="VGE59_00330"
     CDS             11011..11391
                     /locus_tag="VGE59_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY1074136.1"
                     /translation="MNFQLKHRQVTCPQELVDSIESKVTRFERLIPENSFLEVELIQH
                     AKAHGDGDKEAEVILDIPGVKPVIRFTAIGTTFLEAVDRIFDQLDEELGRRKNREGDH
                     HVAVSPKEWSAEEAHKEAVAPTEY"
     gene            11898..>12091
                     /locus_tag="VGE59_00335"
     CDS             11898..>12091
                     /locus_tag="VGE59_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074137.1"
                     /translation="MIKLFSSLIKDTTRGDVQKLQPVIRAINDAESGIQALSDQELTS
                     QFTALRQEIRTKYEELVAQKP"
BASE COUNT         3259 a         2507 c         2929 g         3396 t
ORIGIN      
        1 gtgctcggtt acgccacctc catgtttccc aagaactcca atcccatgcg catcgtcaac
       61 catcagtttg cagccagtcc tttttgctaa ttcaacgtaa gcggggatat taataatttc
      121 accatcaagg ctaaatacac tttccgttac aattagtttg ttaggcaggt cattttcttt
      181 aatcttttgt tccaagtctg ctaagtcgtc atgtttaaat acgacttttt tagctttgct
      241 taagcgacag ccgtctacga ttgagccgtg gttatactca tcgctgaaaa tgacgctgtt
      301 ttcggctaaa acaggcatgt tgttaaataa aggatccata actgcgcgga agactgccgc
      361 atttgccata taacctgttg gaaatgttaa acaatcctcg gttcctgtta gttctgcaat
      421 ggctttttct aagtcctcaa taatatctat atttccacta ataactcgag atccgcctgg
      481 tcctacacca tatttgtcga tggcttgttt cgcagcctct tttacagcag gatgctgcgt
      541 catgccaaga tagttattcg aacaaaacat aaggtagtct tcgccgttaa ccttacagac
      601 tggttcgttc acgcctcctt cgatagtgtg aaagtcgggg tataacccca tctgtgaaaa
      661 ttgatcaaca aacagttcga ttgggtctgt cattttcgga tttcctttcg gcggtcaacg
      721 atacgttttt cttttagttc ggtttctagc ccgagtagtt ggataacttc tttaaggggt
      781 tttgaattga tggttgcaga aatacccata tcacgctgaa gcaatgattg caaaagtgcc
      841 atatcattct cgcgaacttt ttcatcagtg aatgcgatgt tgagaataag ctcatcaatt
      901 tcataggggt catcattctt cttacgaact tcggcttgcc actcttctat ggaagggatc
      961 tcgtgcataa ttgaatggag ggcgtttaga tttaccaatt ctcctttaac cttcgttagg
     1021 ttaagttcga ccatatcgga acggcgttca atcgaagagt gaagacgtgg tgtcgtacgt
     1081 ccgcagtggt cacaaggggc tgtgataatc atctttttac aaaaatcccc agtacgatac
     1141 cgggtaacca cagaaccacg ccaaccgagc gatgtgtata caatctcacc agaactgcct
     1201 tccttgacgg gattgccgtg acggtcgacc agttcgatca tttccaagtc tggataaaga
     1261 tggtagcctg actcttcatg acattgcacc caagcggttt ttgcttctgt gagagcgtag
     1321 gtagatagaa ctttggtgtg ttttgcgcct agcttattga gaaggctttt tacttttcgt
     1381 cgtaatccgg gacttacgcg ttctccacca aaaactataa ttcgaagtga cgagaagtta
     1441 cgtttttcag ccactgcctc acgaaggaga tggtaaccgt atcccggcat gataataagg
     1501 atggtcgctt gaagattttc gatcgcatcc atgatttttc ttgtgcctaa aatttttcct
     1561 ccaccggttt ggagggtcat catattttta cttgtggttg tgttgtacgc aagccaaaag
     1621 gcgaggtgag gtgcaaaggg aaaggcgtta atagcaatat cgtcggaggt cgctccgatt
     1681 gtttcgaata ctcttccgcc gctttctcgt aatgtttcta aatctttcgc tgtataaaga
     1741 aacggaatct gactcgtgct gcgccccgtt gtaaaatgga tatggaccgg tttaaattcg
     1801 tggctaagct cagcttttac gtcggtacca aataattttt ttaagcccaa gctgagtaca
     1861 gcgcttttcg gccaggcttt tcgaataagt tcaatggaag gctgaagaat aaaatcgcga
     1921 gaacgctttg gatttgtctc tgtgggtagg aggtcgagtt ttgaggtaaa aggtagcttt
     1981 tgaaaatctt tgagggaacg aactgagagg tactttgatt tctctggatg attttgaaca
     2041 aaaggttgaa aagggagtaa aaaacgagaa acgtaacgaa gattggtaag ttggatatct
     2101 ttccattctt tgattggttt atacgccagt ttgttccatt cagccataat atttcctcgg
     2161 gtattttctc gattatatca tatactaaga agcgttttcg ctatttttga tccggttaaa
     2221 aaaatcaagg atgtaggtgt aaacagagtc atctttatgg agtgagtgat gaggttgaga
     2281 aatccatacc agttgcttat caatagctgg aatattttga aaaaagtagt taccgctttt
     2341 actattaacc aacccatcat tgtcagactg gataataaga gttggtgact tgatattctt
     2401 aaggtaaaaa ggtgtttcgt cttggatgag gtggagggca tcactaatac tttttagttg
     2461 gagtgaccca taactagcgg ctggtcctcc gatgagaagt ccatccttaa gttctttttt
     2521 aaaggatgcg tggtaatact taatacctag gaaacgtaat acgaacatat agaaaacaat
     2581 ccatgtccca cgttggagcc atcttggagt tcccgctaaa ataaggccgt caacttttct
     2641 tttattcgcg aggatgcatg ccaagtttcc tcccattgag acgccgagaa tgatcacatt
     2701 gttacagtat ttttttagat gtttataggc ttcgtctgcg cttttgatcc aatcttccgt
     2761 atcatagaga gctaagtcat cgggtgtggt tccgtggcca ggtagaagag gggcgtagta
     2821 actcaatccc tgcttttcaa gaagacgagt gagttttgaa aatacaccag gggtgcttga
     2881 aaatccatgc agtagtaaaa cacctgtttt cgcgtcaggg tggagatatt ctagcgtcga
     2941 gtgaagtttt gtttcatgcg tcataatagt gaatagttat agtctatcaa ttggacggta
     3001 cttactcaag cgtttccttg ccggtttgac agctcttctg aaaagcgttt atataaaata
     3061 aaggagacca tatgggagaa gaaggaacta ttggtaaatc agtattgatc gtcgatgacg
     3121 atatgacctt acgcgaaatg tatgaagaat atcttaaagc ctcgggttac ctagttgtta
     3181 cggctcaaga cggtgaagaa gggttagcgc aggcaaagca gagtaaaccg acagcaattc
     3241 ttttagacct catgatgcca aagatgaacg gaattgaagt tcttcggcgc ttaaaagctg
     3301 atgaagaatt aaaagaaatc cctgtgattg tgtttactgc gttaattcaa gatctggaaa
     3361 agcaagagag ttttgcagcc ggtgcctctg actatgtcgt aaaaacagaa gtaactccaa
     3421 aatctatcct gacaaaaatt caagaactca caacatcaaa gggcgaaaac taaacgaagg
     3481 ggaaatcgtg aacgataata caccctccga tcgcggaata cttcgtccac gcagacttgt
     3541 tattgccgta ttagtggttc tttttattgg acttattgtt tttgtcagtc agtcgatttt
     3601 tatttcggcg gatgtattaa ccgtaccaga ctataatccg gcagagctaa aacaacgtta
     3661 tcaaacgaca ataagtgcca gcccaagcgt tactgcttct ggctccccct tagcggctgt
     3721 ctcgccaagc ccgcttgtaa ctccttaaag acaattaaga agtcaggatt atttttagta
     3781 cgattttggt ttgagggctt caagaaaaac attggtggga aggcttactt tacccatttg
     3841 tttcattttt ttcttgcctt ccttttgttt gtcgagcagt ttattcttcc gagttacgtc
     3901 tcctccgtag agcttagagg taacatcttt acgcatggca gaaactgtct cacgtgccac
     3961 aacccgggcg ccaatcgtgg cttgaatact aatttcgaac atttgttttg gaatcaactc
     4021 ttttaatcgt tttgtaagtt ctctgccacg ggcttctgct tggctaacgg gcacgatttg
     4081 agtgaacggg gctaccttat cgccagcaat aaggatattc atttcgacaa gttttcctgc
     4141 gcgatagccg atcaagtcgt atgaaaatga cgcgtatcca gccgatacgc ttttgagttg
     4201 atcgctaaaa ttgatgatga tttcggagag ggggaattcg tactctaaga tggccgttgt
     4261 atttttgtct tcaaggtgtt caccaatgta ttgtagcgag agatagatcc cacgatggga
     4321 ttcggctagt tccatgatac cgccgatata tgctggctgg gtgataattt ccgctttgac
     4381 ccaaggctct tcatatccat tcatcgcatc accggtgtgg gcgacaactg gggtcgtaat
     4441 gactaaatct tggttatact cacgttctaa acgttcttta ataatctcta agtgtaataa
     4501 acccaagaag ccaacacgaa atcctgaccc gaaggcatca gagtagtgtg gttcaaaagt
     4561 gatagaagca tcattggctg agagcttttc taaggcagtt cgtaaggctg tcgtgtctcc
     4621 gtctgagtgg aagagggagg caaaaaccat tggctgaggt tggcgatatc cttcgagggg
     4681 tgcggtagct cgtccggaga catgggtaat cgtatcaccg acgcggcagt cggctacgcg
     4741 tttaaagccg gttacgatgt acccaatatc acctgcttct agtttttcga cgggcttgtg
     4801 atcaatcgtc atgacaccga cctcttcaac ggtaaaggtt ttgccgttgg ccatcatcac
     4861 aacgttttcg cccgctttta tagatccttc cataatccga acatgagcaa ttactccccg
     4921 ataggaattg aactgggaat caaaaactaa ggcgcgaagg ggcgcattga tttcttgttg
     4981 tggctgagga atcgcatgca cgactcgttt taatatttca tccaaaccga gtccgctttt
     5041 accagatgcg gggataacag aatcagggtc acacttgagc agatctgcca gttcaaaggc
     5101 aactttttct ggttcagctg cgggaaggtc aattttatta acgacgggaa caatcgtaag
     5161 attatgtttc tgggcgactg ctaagttggc taaggtttga gcttgaatgc cttgggttga
     5221 atcaaccagt aaaatagcgc cttcgacagc ggcgagagag cgagaaactt cgtacccaaa
     5281 gtccacgtgt ccaggtgtat cgataagatt gagttcgtaa tcaactccgt ccaggtgata
     5341 cttcatatgg actggttgga gcttaatggt gatgccgcgt tctcgttcta agtccataga
     5401 gtcaagcatt tgctcgcgaa tcttattttt ttcaattgtt ccggtgtatt ccaaaaaacg
     5461 gtcggctaat gtcgattttc catggtcaat atgcgcgata atacaaaagt tacgaacctg
     5521 ttgcataacg ggttaattat accaaattat gtctatggag tcactctagg cctaataagc
     5581 cccaggccga aatcagaact aggttataga tggaatccgt ccgataggta tatcgcgtcg
     5641 gggtgttcgt agttccactc tgtaatacag tcctgccaga ccatatttgc atttgatagt
     5701 tgttgccagg ctgtcatagc ggtattatag ctgtttaagc tctgggtata ggctgtaaga
     5761 gcggcgtagt aagcgtttat gtcgggataa gcgttaatgt cgtaattgaa tgccgctggt
     5821 ggcgttggca ttggtcccgg ttttgcagga ggcgaagtca tcccgccggt acagttagcc
     5881 aaagtgcttt caaagtaact tagggcaggt gagggggtgc tatgaggtaa tttatcgcat
     5941 tgtgaggcag gaaatcctgc ggctatgcag tcttcatagg tcccgtcata gttagtgaag
     6001 ttattagaag gggtgggaga gggtaaagga gttggagtga cagaggacac aaatggtgtt
     6061 ggagtggttg atttgagaag gtgctcacat tgtgaggcag agaatcccgc tgctatacag
     6121 tcttgatacg ttccatcata attagttagg tcaattgaag gagttggtga cggtgtagga
     6181 gtgggagttg tgaatttatg aggtagccct gcgcaatctg aagcagaaaa accagcagag
     6241 atacaatctt catagtatcc atcgtaacca gagaatgttg gagaagcgtt tacaagtggt
     6301 gtaggcgtta cggttaccgt tggactaggg gaagaaagta gtcctctatc aagaaggagc
     6361 tgacaccaac tagcacgtcc tgggtctcct gctaaacagt cttcgtacgc tgtgtgttga
     6421 tttgggactg gggaagacgt cgtagccgtg agtgtgtcgg cacttggttg cttgaccaga
     6481 gcggtaaagc taatggtcac gagtataagg atgattacaa taagggaact aagaaaaatg
     6541 acttgtttat tcataatggt tccttatttc cataccttgg ttggtgcacc atcggagtat
     6601 tgctgggaaa tttgctggtt gtagcacttt tgggtgcttg tgtggagatc ctgcatccag
     6661 ttgatgcctt ccaggtagtt tgcacgaaca cgtgatacgt atgccgctgt ctcgcttgtt
     6721 gctgcactgc tgctaggaac agaagggaga ggggcggggt gttgggcaat tgttggcgcg
     6781 aggcatgttt ttacggcaac actatttaaa atcttagtat caacgttttg agggatagcg
     6841 agctcgggga cgggggggag tgctgaagtg cttttatttt gagtttctaa aagataagaa
     6901 tttaaagcga tgctaaaaag caaaaaaaga ataagcgcag caagcaaata aataagtttt
     6961 ttgtgacgtg aaatccagga catctctcct cctctatata agaatatagt agatgtcttt
     7021 agccgagaag gcaagtataa ctttaggcga ggccttcgtt ctgagccgta tcttcactgg
     7081 aggggagagg aatattttta acagctcgtt tcggtgtaat ccactggagt tcttctcgtt
     7141 tgagaagtag gctgatcgca agataggtta atccgcccgt cagtgccgca gagagtaggt
     7201 taataaagag agggataatt cgtgttgttc caagaccggg agttcccaca aagactccat
     7261 cactcacaat agaaagggtg actcgtgcga caagtcctga ggctaaagaa ataaggaggg
     7321 tcatagtaat tgtatcgagg agggaacgta agttaagttg ttttcgatac gtcgattgga
     7381 aaagagtaaa gagaattaat gactcgagaa tcgcggccag tgcaaatgcc atggagagag
     7441 cccatacgtc gccaatgcga tttgctaaaa tccacgcgag aataacggca actgcgcccg
     7501 aaaagagcga cacatacatc ggacgttttg tatcttgatg agcataaaag gcacgaatca
     7561 taataaggtt aaatccttgg gatgcaaggg caagaataaa ccagctaaat gtgttgatgg
     7621 ctcgaattgt gtcttcccaa gtctgatgat tgagggcgag gtagagacga ataatatgag
     7681 ctcgcagaat ccacagtaag atcatcgaag gaatcataaa gaagaacatg ccctttaatg
     7741 cgtgggtcag aagggattta tacttttcgt tatctttctc acttacggcg gctcctagca
     7801 tggggaacac agctgtgcca atggaagccg cgaaaattgc cgttggggtt gtttggaagt
     7861 tattggtgag attataaata gagatcgctc cagcaggtaa gagagacccg atacgtgtaa
     7921 atgccaagag aataatttgg gacatgccaa ggctgagtga tcgtgggatc attaaacgga
     7981 tgattcggcg taaagaggca tccttaagat tcagtacgaa gtgatatttg tatccgagtt
     8041 tacgtagtgc cggaagttga accaaagcat gtaagagggc tcctactaca accatccaag
     8101 cgacaatcgt aaccgtaaag cctggggcaa gaagtcctcc aacgataatt gaagcgttat
     8161 acacaagagg tgccaacgcg taggagaaga aacgtttgtt ggcattgagc atgccaccaa
     8221 agacatacga gatcgcaaaa aatatgggtg aaaaaagaag aatccgtgct aattttacgg
     8281 tcatcgcgta cgaagaaata atatagcctg attcagcttg gagcgtatcg gtagtggaaa
     8341 agccggggac aaagaagtgc aataggtttg gcatgaagat atacaagcca acacagagaa
     8401 tcccgagaaa aataagcgaa gtggtaatta agttattgga taattcccac gcagctttgt
     8461 cgttttcttt ggttttgata tcgagaaaaa taggaataaa agccgagctg atagctccaa
     8521 ggataaagag attaaaaaga aaatcaggaa tacggaaagc tgcgtaatac gtatcaagaa
     8581 tatgtaacgg gatagcattc gctaaaatca agtcacgtaa aaagcctaag atattactca
     8641 aaagcattgt aataacaagc aggcttgcgg cgccccgcaa gtcctgctca ctatgccata
     8701 atcgtttaag tccagaaacc atacgttcct atcctaactt actttcgcgt gcctgcaaag
     8761 cgcagggatt aggcgtcggc gtttttggat tttggagtac gtttggcggt tttctctgat
     8821 tttactgttc gttttgttcc gccgttaaat tcgtcgtaca tagcgtcgaa ttcctcgcct
     8881 tcgatcgttt cgatcttgag aagacgctgt gcggtagctt cgagaagggc agattgttcg
     8941 gtcaggattt tcttcgcgcg agccatttgt tcatcaataa tttctttaac cgtcgcatca
     9001 attagctcgg cttgcttttc tgagtagttg cgtacttcag cgccgcgtcc aaggaaacta
     9061 tcatgacgtt ctccccatac cacatggccg agtttgctca tgccgtacat catgaccata
     9121 tcacgagcaa tcgctgtggc acgttcgaag tcgtttgaag cgccggtggt aacttcctta
     9181 aaaataatgt cttcggcagc tcgtccggcg aggagttggg caatttcatc ttcaaagtga
     9241 ctcttcgaag acatgcggct ctcttcttcg ggaagactcc atgtataacc aagtgccata
     9301 ccacgagaga cgagcgaaat tttatgaatt gggtcaccgt tgggaacatt tttaccaacg
     9361 attgcatgtc ctgcttcgtg gtaagcggct acactacgtt cctgggtatt catgagatgg
     9421 gatttgcgtt ctggtccgat caagactttt tcaatggctt gggtgaagta ttgctggcca
     9481 acttcttttt tattctcacg cgcggcgaga atagcggctt cgttcacgat atttttaagg
     9541 tcggaacccg aagctcccgg tgtttgtcgg gcgattacag ccagatcaat gtttttcgct
     9601 actggtttat tgcgaacatg aactttaaga atctcttccc gttcggaacg gtcggggagg
     9661 tcgagtacaa ctttacggtc aaatcggcct ggacgaagta aggctgggtc taaaacatca
     9721 ggccggttag tggctgccaa aataataact cgggaatcgg tttcaaatcc gtccatttct
     9781 acgaggatct ggttgagggt ctgttcccgt tcatcgtggg atccgcccat accaacgcca
     9841 cgttggcggc caatggcatc aagctcatcg ataaagataa caacgggagc cattttctta
     9901 gccttggcaa ataagtcgcg tacgcgggca gcaccaaccc cgacaaacat ttcaacgaat
     9961 tcactggcag aaagggttaa aaatggtacg cctgcttcgc cggatacagc ttttgctaag
    10021 agagttttac ccacaccggc tgggccgacg agtaagacgc cctttggaat ctcggcgcct
    10081 agtttttgga atttctcggg atgcttaagg aagtccacaa cctcgacaag ttcttgtttt
    10141 gcgttcttaa gccccgcaac atcgttgaaa gttacctttt gggttccact taccattcgg
    10201 gccgttgtct tggcaaacga catcgctttc atgttcccgc cttgggcatt acgatacata
    10261 aagaagataa atccacccaa gagaagaacc ggtaaaataa ttgggatcaa actaagccag
    10321 aatgacgcaa ctgagttatt atctacttct aaggtagcgt tttcgggtgt aatgccataa
    10381 tcagtaatac taatacccgg ctctttgtcg gcacgaagaa tagagccatc ttttagtttg
    10441 gccttgatcg agttcccctc gaccgttact ttctctacct tctcttcctt aatgacttca
    10501 ctaatacgag taaggttgac ggtttcgact ttgggacggt ttgggttgcc aaaaaacgta
    10561 tagagaattg aggcaaaaat aataataccc aggaaataca gtagattttt cattcaatcc
    10621 cttctcttat gaaacaagtt cttaaatctt ctctttttgt gcattcctac tttatcacaa
    10681 ggctttttaa atgtcatctc agcaaaagca caaaccactt ggcttctggc tctctattat
    10741 taaggaaatg ctactacgac cgacggctgg agcagggtac gtagcgcccg tgaagacggg
    10801 acgggaagta acagtcctaa agccttggct ttcagccgga gtcagagggc aggggactta
    10861 atttccggaa gcagtcccct accctccaac acctcccacc cccgcaccgg acaaggatga
    10921 ccttttatag ttttaaacaa aacgctcgtg aggttgacgc tcgacttttt ttcttaatta
    10981 tgatatgcca taagcaaaca aggaggtagg atgaacttcc agcttaagca ccgtcaggta
    11041 acgtgtcccc aggagcttgt cgactcgatt gaaagcaagg tcacacggtt tgaacggctt
    11101 atcccagaaa actcgtttct cgaagttgaa ttaattcaac atgcgaaggc gcatggagat
    11161 ggggacaaag aggcagaagt cattctagac atcccgggtg taaagcctgt tatacgattt
    11221 acggctatag ggacgacctt cctcgaagca gtagatcgta tttttgatca gctagatgag
    11281 gagttaggaa ggcgtaagaa tcgtgagggg gatcatcacg ttgccgtatc gcctaaagaa
    11341 tggtcagcgg aagaggctca taaggaagcc gtcgccccta ctgagtattg acctcgcgtg
    11401 ctattcgtga tatagtaagt gcaccccatg aagcttgccc agcaagtatg actgaggcgc
    11461 actttataat ccaactcctt ccctttgtgg gaggaggaag tagagcataa cggctctttt
    11521 tccttctctc actcagctct tattgagaga tagttgtgta gtgcgagtac caaatctggc
    11581 aacaagggtg aaaaacgcct aatctgccgt cttggtattc gcttggtccg gagcgcacga
    11641 ggatccattt cctcgtaagc gtgttgactc agcgtgcagc acatgtccac gcacgctcct
    11701 ggaccatgct ccccagcggg tctcgattga tcgctgggtg gagccgccgg gtgaggccag
    11761 attcctccct ctgtcgaggt tggattcttg gcctccccgg ctcatctttc cactctgccg
    11821 cccttttcgg gcggctttcg ttatatatag ggagtatgac tttctttttc aggtaaattc
    11881 ggtacaatgc gatagttatg ataaaactct tctcttccct gataaaagat acaacacgcg
    11941 gtgacgtgca aaaattacaa ccggttattc gagctattaa tgacgccgag tcgggaattc
    12001 aagccttaag cgaccaagaa cttacgagtc aattcacggc tctccgacaa gaaattcgca
    12061 cgaagtatga ggaactagtt gcgcaaaaac c
//
LOCUS       DASWYB010000006        16006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_771502, whole genome shotgun sequence.
ACCESSION   DASWYB010000006 DASWYB010000000
VERSION     DASWYB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16006
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771502"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1024
                     /locus_tag="VGE59_00340"
     CDS             137..1024
                     /locus_tag="VGE59_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074138.1"
                     /translation="MKGQGKTRMLPIGLYFKLEGDKWIATLPDYRESERDRNAQELAA
                     RERVIGVLESCAEVRMDLASHSRLRRPAIEFAIRPFRDGWGYLMGNLGDMWPSHFLFL
                     RAVMENQDGLLQGNAAYAFLKQFYHRFLEHHPRVFPDHEEAPLETQVRYCATQRKLFL
                     KCPRYTGILIGKEGVHIRSVQQAFRSVGVKVEVDSIKPVMVDEARTLQVARLLVETGC
                     RVTSRVERTGGAHWEYSLWDVCRRVDPARGAGNSSEVRHTLAWMIEVCVGVEALHERY
                     PDNDLLDEMAGLTDRLSAE"
     gene            1126..1608
                     /locus_tag="VGE59_00345"
     CDS             1126..1608
                     /locus_tag="VGE59_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074139.1"
                     /translation="MLLVLLQIVAVLLFYRRPSVRLSYHDRDECAVVASMIGDLYTSL
                     KTKQEVPLSSRLYFLAEEYADSENLYGGNAGEAEVCDDTLLARREKRLHQLLVAALWR
                     AGRVQGFRLPVSLGYQSWKIDRVRNQEELLLTAAAQVSQDFVQLGGTLTAAKPQAVQS
                     "
     gene            complement(1757..1948)
                     /locus_tag="VGE59_00350"
     CDS             complement(1757..1948)
                     /locus_tag="VGE59_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074140.1"
                     /translation="MRLGLRLLQSPDDSGAREEYPPRSAPDSATVSESSANLDPVEED
                     SATNGGEEPKGGSDEVAGD"
     gene            complement(2114..2710)
                     /locus_tag="VGE59_00355"
     CDS             complement(2114..2710)
                     /locus_tag="VGE59_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074141.1"
                     /translation="MIQVRLLAMLHSASCFGTFSASAQQKTMQHYLIDSSVVTPLSEL
                     MEQAARMRWCMRRLGSKATVRIAPDQRPTHHQVMGWAWNLAYWTMEAIDEGAIKKAPN
                     QPRWFRRVKGLVTDEDLTPFTCPAETLYLLDLTVVYLSACLNLRYDSNRTWHMTFRGS
                     VSLHKWLGFLVEFGDGPARRVHVAHDMDEAGVRLEKQE"
     gene            complement(2795..3616)
                     /locus_tag="VGE59_00360"
     CDS             complement(2795..3616)
                     /locus_tag="VGE59_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY1074142.1"
                     /translation="MWQRFTERARRVVFFAQEEAARLGENYVGTEHLLLGLIRESDSV
                     AGRILDQLGIPLVRIRADIERQVTRGNGNLGQEMQLTHRGKRVFDFAYEEARLLDDKH
                     VGTEHLLLALIREEDGLAARVLKKLGADLARTRREVYHMQSGAPKVTPLLASPEATRA
                     LIDVYDQLTDDARSRLERGVAFSDIGPELQAAVQNACLNRGPLSPIPDGSVIYLRPER
                     SDEVTTAVEISIQITTVVLATLSPAPKQPEAAAASPREESTVDPDSTEDTAHQTS"
     gene            complement(3749..5035)
                     /gene="eno"
                     /locus_tag="VGE59_00365"
     CDS             complement(3749..5035)
                     /gene="eno"
                     /locus_tag="VGE59_00365"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY1074143.1"
                     /translation="MAQIQDIRAREIFDSRGTPTVEVDLYLNDGSMGRAAVPSGASTG
                     AYEAVELRDEEKAHGGKGVKKAVANVHDILLPALKGKEADQVLIDELMIELDGTHNKG
                     KIGANAILGVSMALSKALAIHRKQPLWEYFRGLSTTVPADYIMPVPMMNILNGGKHAD
                     KSTDIQEFMIMPVGASSFSEALQIGAEIFAALKKVLKKEGFSTTVGDEGGFAPSLKSN
                     KDALEMIKRATEAAGYELGKDVVITLDVASTELHEDGTYHLASEGKKLTSDEMIDTYV
                     EWCNEYPIASIEDALDENDWEGYQKITEKLGNKIQLVGDDLFVTNVERLQRGITDKAG
                     NAILIKLNQIGTVTETVRAIDLARQNGMNAIISHRSGETEDVTIADFAVGLSAGQIKT
                     GSMSRSDRVAKYNQLIRIEEALGDKAVYPGNSIFKK"
     gene            complement(5257..5721)
                     /locus_tag="VGE59_00370"
     CDS             complement(5257..5721)
                     /locus_tag="VGE59_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074144.1"
                     /translation="MPHIHDLIDWTVTAFIVHTDRVLLVHHTALQKWLPVGGHIELHE
                     DPEQALLRIVQKEAGIEFDLLSPRSEVPVSGGRMLPTPNYMDIHAISDTHKHIALGYY
                     ARGLSDQVTLEKDAHSEIKWFTREELNDPKFDLLPNIRINCEEALDKVAATL"
     gene            complement(5793..7007)
                     /locus_tag="VGE59_00375"
     CDS             complement(5793..7007)
                     /locus_tag="VGE59_00375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1074145.1"
                     /translation="MQQTTALTKSAITFSLIKNRHVGQFTEGYPLTNGVTVAVDTLVQ
                     NLPSNFKSTIVAPEAGNQNDPADVLRIPAIGELWEYPIAQPWRTKHLVHYFANNGLDL
                     VHVHHPFLMGMVGAKIAKKFKVPLVAHHHTLYETYGHYVKLPTSISDRIAKYGLGPWL
                     HWYYDIFAECATIIVPSKATETSLRQMGVEASIRVIPTGVPLPKSEDISPAKQQEIRC
                     RYGIKSEDFLLLYVGRLAKEKNLDLALQAFDALANEHTNLHFLFVGSGPEEESLREQA
                     ALSVAADRIHFAGRQPRELLDPFYAAGDLFVFPSTTETQGLVTAEAQVAGTPSVVVNV
                     GGACELVTDGMDGLVTAPTVADLSQSIRSILTNPTLHATLKQGCITNRSKRTPAAMIE
                     QVLDVYESVLAR"
     gene            complement(7117..7530)
                     /locus_tag="VGE59_00380"
     CDS             complement(7117..7530)
                     /locus_tag="VGE59_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074146.1"
                     /translation="MVRYRHRGRERRVGSIIVVEGTERPIPVPDYTEAKRKLGLLLAG
                     GLWPMEKARSAQTYLEWFERNLYVDEYENLRHRACGKRVDNDVRCTSRTCYGLIVVPP
                     PAGLVEALHRLGEVDDSAYAGMTERAVPPTPQHIV"
     gene            complement(7602..9371)
                     /locus_tag="VGE59_00385"
     CDS             complement(7602..9371)
                     /locus_tag="VGE59_00385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY1074147.1"
                     /translation="MTQQSITPTWNLNDLYSGMDDPKIEEDFSAAEALVRELQDYRGK
                     IATLNPSEMLTLIKSQEALALKSNRLGVYASLLSATNVEVPEVTRFEKQLDARMIQLG
                     KETIFIEVEMAQLPEPKWQEFLNAPELNAYKKTLEDTYREAKHTLSEPEEKILAEKSQ
                     TSSQALLHLFSITTDTLMVDWNGEKKPLEEISPYLRDPDKAIRKQAVFAIHAALNTNS
                     KTTPAVFNALIQDKEISDRLRNYDYPEERRFNSDDVEKDTVYALIEAVNKSSHLVERY
                     YTLKKKILGEDTLHWWDRYAPLPKPKKEISPEEGKEMVLAAFEEFSPQVREIANGMFE
                     KAHIDWFPSNKKRGGAFCQFGTPESYPYVLLNYTNDVNDVMTLAHELGHAVHDVLVKD
                     TNVFFQMHPSLALAEIASTFSELLLFDKLLAGDLTKEDKIALLMGQIEDNFATVHRQI
                     AMFQFEQAVHTKRRTDGELSKDQIDDLWHEIIKKPFGDSLVWTDEHKNFWMYIPHVFN
                     WPFYVYSYAFAQLCTLAIYKTYKEKGSEMVENYLDILRRGGSMAPKDNLALAGFDVST
                     PDFWQNGLKMIEDYIAQLEAELA"
     gene            9506..9706
                     /locus_tag="VGE59_00390"
     CDS             9506..9706
                     /locus_tag="VGE59_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074148.1"
                     /translation="MVAALWIATIGIIADHVFIGMELRRCHARFNRTQFWFEIVRTHS
                     CFLHYYPYLSDLWERDGKPELP"
     gene            9830..10105
                     /locus_tag="VGE59_00395"
     CDS             9830..10105
                     /locus_tag="VGE59_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074149.1"
                     /translation="MPYPVKAVVAFALFFVLLLGDLLSALLHWVDPTALNRWGALDGL
                     LRQCEDTYNGLVESIRPSPSKSAQVVACAPSLDKVEVPTEPISFVEF"
     gene            10256..11965
                     /locus_tag="VGE59_00400"
     CDS             10256..11965
                     /locus_tag="VGE59_00400"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY1074150.1"
                     /translation="MATKTIFITGGIVSGLGKGITAASLGALLKARGFSVIPMKTDMY
                     LNVDAGTMNPFQHGEVFVTEDGAETDLDLGHYERFLDSDLSRLSYSTMGSVFQAVTEH
                     ERKGTYLGKTIQIVPHVTDEIQRRFQTISETAQPDFQIIELGGTVGDFEAEVTLEAVR
                     QFQTRNKRDCISIHVVKMDYIYPSDEAKTKPMQHSVIALRRYGINPDILVVRAKRPIS
                     QDEKEKIALFCGIDSDHIISALDVESLYDIPLNMEKEGLARVTLSLLGIKESAPNLAE
                     LEEITRRTKLTEPVIRVGLVGKYTDLSDAYLSVIEAAKHGGIANSVKVQVVPVDSEHL
                     NVQLLKEVDAIIVPGGFGTRGVEGKIKAIQYARENKVPYLGICLGLQTAVIEAARNLA
                     GIKDATSEEFDEKANHPVIAFLPDQKGITRKGGTMRLGSYEAVMADSLISRLYAWWED
                     KKAFDFPSLDEVMKFKPVKTSLERHRHRYEVNPDYHKVLQEAGLRFSGMSPDGSLVEF
                     IEYPREVHPYFVATQAHPEFKSRPYKAHPLFAGLIAAAKEQSQNHTTLAQMPVDSVQT
                     VAS"
     gene            12031..13122
                     /locus_tag="VGE59_00405"
     CDS             12031..13122
                     /locus_tag="VGE59_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY1074151.1"
                     /translation="MSHVSHNYAVIIAGGTGARLWPVSRKSKPKQFHPLLGETSLFQG
                     TYKSLSNCLPTSNIIIQVNQELIPLVKEQIPGIDESNIIFEPVGRDTGPAYLFAAATV
                     AQRDPEANVAIFWSDHIIKNQANFKSALETVFTTLNENPERIVSLGVKPDFPHTGLGY
                     IALGQKFKEQPTGTVFEVERFVEKPAYDKAVEYLATGNYLWNTGYSAFSVAGFESVLQ
                     SADPTLATHYSEMKHLIEQNNVESLREFFESLPRISIDYWLMEKLTNMLAVPAELEWS
                     DVSNWKTVYDLLSEAEGTDMVTRGDHISYDSHNCLVIGNKRLVVTAGVENLILIDTGD
                     VVFALNQNSVHEMKEVVALIKESKFSDLL"
     gene            13234..14310
                     /locus_tag="VGE59_00410"
     CDS             13234..14310
                     /locus_tag="VGE59_00410"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY1074152.1"
                     /translation="MSPLDNRAIIKQLDTDNYLSHLLDAPEQIVDGFHLGEEITIPAL
                     YAQAKQVVLLATGEMLPVALAVQALAVEYARVPVVIVFDYVLPHWVSSDTLVIALDYY
                     GTTEQVLVAFREAASRRARLLAISVGGDLSREARRVRAVHVPLSYGAPARAAFFYAFS
                     CVAYIFRKLDLTELRESTVTESSVLTRSLLESIGPEVAQYQNNAKQLAEKILTRKTIV
                     VGSGPLIGIAKRWQLALAQNSKAVITSSTLNEFNDTIINGLGYFSKPIEAPFVIMLQS
                     KYDHPRNKLQQTLTYQVAQAQKIIYEQIFMHPSGSLFGEIVLAALLGDMTSYYVALLS
                     HHNPSPLDATDYIRGRLKEEKLFE"
     gene            14339..15550
                     /gene="eno"
                     /locus_tag="VGE59_00415"
     CDS             14339..15550
                     /gene="eno"
                     /locus_tag="VGE59_00415"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY1074153.1"
                     /translation="MAVIASVAARVINDSRGQPTIEVTVTDDKGRIATASLPSGSSLG
                     KYEAVTQDAQQVVNSINKVIAPALLRQPLTDQATIDAKLRSFDDTANHEKLGVNSLLG
                     ISLACARLLAQDHNLSLYHYIFTISGGPGLTIPTPMFNLLNGGKHAENNLDFQEYMVI
                     PIGFKTFHEKLAAGKKVFSALGTILSETGHITKIGAEGGYAPHLSTNEEGLGVLVQAI
                     QSAGYEPGKDFVLGVDVAASALPPTYSATVDNYLALYRNFPLFSLEDPFVEDDWESWA
                     KLKVGVDGLSEPGNPRLLVGDDLFVGNKDRLLEGVKKFSANAVLIKINQAGTLTEIIE
                     AIKIAREHNYVHILSHRSGETLDSFVSDLAIGTAAAFIKAGSANEQAPERIVKYERLV
                     EVEEELNIGHI"
     gene            15547..>16006
                     /locus_tag="VGE59_00420"
     CDS             15547..>16006
                     /locus_tag="VGE59_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074154.1"
                     /translation="MKATHIAVITHPRAHNSLAKRLEKTFRVGKLDGIYKLPYSHSPL
                     SPAGTSPYSVLYSLSHPSKELTHLKKLEPVHVRQLRDIAYRCRKSSGTLHIIGVLDDD
                     SPFGKRRMVEALVRHFRPTGLKLVLHLGVWHPTPTEFRYGLKEIRSLCNES"
BASE COUNT         3855 a         3749 c         4073 g         4329 t
ORIGIN      
        1 ttgcgccgcg taaggggggc gcatcaatca gtcagtcagc aacgccgtgg ggacttcggt
       61 tcccacggcc ttaccttact cagcccaact tggactgagt aatagtaagc agtttaaatg
      121 ttattagtac tcggctttga aaggccaagg taaaacgaga atgttgccga tcggtttgta
      181 ctttaagctc gagggcgaca agtggattgc cacgcttccc gactaccgcg agtcagagcg
      241 tgaccgcaac gcccaagagc tggcagctcg ggaacgggtc atcggcgtct tagaatcctg
      301 cgcagaagtg cgcatggatc ttgcgtcgca ttcccgttta cggcggcccg cgattgagtt
      361 tgcgatcagg ccgttcagag atggctgggg gtacctcatg ggtaacctcg gcgacatgtg
      421 gcccagccac ttcctgtttc ttcgagctgt tatggagaac caggatggcc ttctccaagg
      481 caacgccgcc tacgcgttct tgaagcagtt ctaccaccgc ttcctggagc atcatcctcg
      541 agtctttccc gatcatgagg aagcccctct cgagacgcag gtacgctact gcgcaacgca
      601 gcggaagctt ttcctcaagt gcccgcgcta tacgggcatc ttgattggga aagaaggagt
      661 tcacattcgg agcgtccagc aggcgttccg cagtgtgggt gtgaaggtcg aggtggactc
      721 cattaagccg gtcatggtgg atgaagcgcg gacgctgcag gtagcgagac tgctggtcga
      781 gacgggttgc cgcgtaacgt ctcgggtaga acgtacggga ggcgctcatt gggagtactc
      841 cctgtgggat gtttgtcgcc gagtcgatcc agcccgcggg gcgggtaact cgtcggaggt
      901 tcggcacact ctggcgtgga tgatcgaagt ctgcgttgga gtcgaagctc tccatgagcg
      961 ctatcccgat aacgacctcc tggatgagat ggccggcctc acagatcgtc tgtcggccga
     1021 atagggtctt tgaccctgac aggtgcggca acggacaggt tcgcttgtcc gttgccccct
     1081 tacttctctg ctctcaagct cgagagtgga gatggtgagg tttgcatgtt attggtgctg
     1141 ttgcagatag tggcggtgtt gttgttctat cgccgtccga gcgtacgtct cagctaccat
     1201 gatagggacg agtgcgcggt agttgccagt atgatcggcg acctctacac gtcgctgaag
     1261 acaaaacaag aggtgccact ttcgtctcgg ctttacttcc tggcggaaga atatgccgat
     1321 agtgaaaacc tctatggggg aaacgcgggt gaggcggaag tctgcgacga taccttgctc
     1381 gcccgtcggg aaaagcggct ccaccaactg ttggtggccg ctctctggcg ggcaggtcgc
     1441 gtacaaggct tcaggctgcc ggtctctctg ggctaccaat cctggaagat cgatcgggtg
     1501 cgtaatcagg aggagttgct gctgactgcg gctgctcagg tgtcgcaaga cttcgttcag
     1561 ctcggcggaa cgttaacggc tgcgaagcca caggctgtgc agtcgtgata ccgctactat
     1621 gctacgtccg tacactcaaa cgccgcggaa ggtttcgacc tcaccgcggc gtttttgcgt
     1681 ataaaaagag ccggttcgtc ctccccttac atgggaaaaa tgaaccggca ctgagtcgag
     1741 tcaagcttga ggccggttag tcgccggcta cttcgtccga gccccctttc ggttcttctc
     1801 caccgttggt tgccgagtct tcttcaactg gatccaagtt ggcgctcgac tcgctaaccg
     1861 ttgccgaatc gggcgcagag cgtggaggat actcttctcg cgctccagaa tcgtcaggag
     1921 attgcaggag cctgagtcct agtcgcatag tgaattcccc tttcgaacct atctctggaa
     1981 tgttgatatt tatgtcaccg aaactagtat ataataaata aaatataatt caatgcattt
     2041 acataaaaat tggttcgtct ctgccctgta taggtaagaa acgaaccggt tatgaaccaa
     2101 accacgatgc cccttattcc tgtttctcta accgaacccc cgcttcgtcc atgtcatggg
     2161 caacgtggac gcgcctggcc ggtccgtctc cgaactcaac caagaatccg agccacttgt
     2221 ggaggctcac gctccctcga aaggtcatgt gccaggtgcg attggagtcg tacctaaggt
     2281 taagacaggc actgagatac acaaccgtga ggtcgagaag gtataaggtt tctgcgggac
     2341 aagtgaaagg tgtcaagtct tcatccgtga ccaacccctt aacgcggcgg aaccaccttg
     2401 gctgattggg cgccttttta atagcgcctt cgtcaattgc ctccattgtc caataggcca
     2461 aattccaggc ccatcccatc acttggtgat gcgtaggacg ctgatcagga gcgatacgga
     2521 ccgttgcttt ggatccgagc cgtcgcatgc accatctcat gcgtgctgct tgctccataa
     2581 gctcactcag aggagtgacg acgcttgagt cgattaagta gtgttgcatt gtcttttgtt
     2641 gcgcagaagc agagaaagtg ccaaaacagc tggctgagtg gagcatagca agcaagcgaa
     2701 cctgaatcaa tactttatat aaaaagaatc ggtccgttcc cctgcccttg cgggtgagag
     2761 aacgaaccgg ttgtgatgac cagcgcgcgg ctggttagga tgtctggtgg gccgtgtctt
     2821 ccgtgctatc tggatcaacg gtcgactcct ccctaggaga agcggccgct gcttcgggct
     2881 gcttgggcgc ggggcttagg gttgcgagca cgacagtagt tatctgaata ctgatctcta
     2941 cggcagtggt gacttcgtcg gatctttccg gacggaggta gattacggag ccgtcaggga
     3001 tcggactcag tggccccctg ttgaggcatg cgttttgaac ggcggcctgg agttcaggac
     3061 caatgtccga gaatgctact ccccgttcca gcctacttcg cgcatcgtca gtgagctggt
     3121 catatacgtc aatgagagca cgcgttgctt ccggcgaggc aaggagcggg gtaaccttgg
     3181 gtgctcccga ttgcatgtgg tagacttcac gccgcgtgcg cgccaagtct gctccgagct
     3241 ttttgagtac ccgagcggcg agtccgtctt cttcacggat gagtgccagc aggaggtgtt
     3301 cggtgcctac gtgtttgtcg tcgagcaacc gtgcttcttc gtaggcaaag tcgaagactc
     3361 tcttccctcg atgggtgagc tgcatctcct ggccaaggtt cccgtttcca cgagtcactt
     3421 gccgttcgat atctgcccgg atacgaacta gggggatccc tagttgatcc agaatgcgtc
     3481 ctgctactga atctgactct cggatcagtc ccagcagcag atgttcggtc ccaacgtagt
     3541 tttcgcccag gcgtgcggct tcctcttggg caaagaagac aacgcgtcgt gcccgctcag
     3601 tgaaacgttg ccacatcgct gtggcctcct ttcttcacag atcaactaac tgatttagcg
     3661 atagtgtgag tatattattt ttatctgttg ctgtaaatgc aaaaaaccgt gctggtggca
     3721 cggctttttg tttacttaca taactcgatt acttcttaaa gatcgaattt ccagggtaca
     3781 cagctttgtc gccgagggct tcttcgatac gaatgagctg gttgtacttg gcaacacggt
     3841 cggagcggct catggaaccg gtcttgattt gcccagcaga taagccaacg gcaaagtctg
     3901 caattgttac gtcttcggtt tcgcctgaac gatgcgagat aatggcgttc ataccattct
     3961 ggcgagccaa gtcgattgct cgaacggttt cggtaacagt accgatttgg ttgagcttaa
     4021 ttagaattgc attcccggct ttgtcggtaa tgccacgctg aaggcgttcc acgttggtaa
     4081 cgaacaagtc gtcgcccacg agctggattt tattaccgag cttttcggta atcttctggt
     4141 agccttccca gtcgttttcg tctaaggcgt cttcgattga ggcgattggg tattcgttgc
     4201 accattcaac gtaggtgtcg atcatttcgt ctgaagttaa tttcttgcct tcagaagcga
     4261 ggtggtaggt accgtcttcg tggagttcag tcgaagctac gtcgagcgta attacgacat
     4321 ccttgcccaa ttcatagcca gcggcttcgg tagcacgctt aatcatttcg agggcgtctt
     4381 tgttggactt aaggcttgga gcaaatccac cttcgtctcc gacggttgta gagaaacctt
     4441 cttttttgag tacttttttg agggcagcga aaatttcggc cccgatttgc aaggcttcgc
     4501 taaatgagct tgcgccaacc ggcatgatca tgaattcttg gatgtcggtt gatttgtcgg
     4561 cgtgtttgcc gccgtttaga atgttcatca ttggcacggg catgatgtag tcggcaggaa
     4621 ccgtcgtgga caaaccgcgg aagtattccc agagcggctg tttgcggtgg atggccagag
     4681 cttttgaaag agccattgat acgcctagga ttgcgttggc accaattttg cctttgttgt
     4741 gggtgccatc gagttcaatc atgagttcat cgattaacac ttggtcggct tctttaccct
     4801 taagagcagg taacaaaatg tcatggacat tagcaacggc ttttttaaca cctttgccgc
     4861 catgggcttt ttcttcgtcg cgcagttcga cagcttcgta ggcgcccgtt gaggcaccag
     4921 acggaacagc ggcgcgaccc attgagccat catttagata caggtcgact tcgacggttg
     4981 gtgtcccgcg ggagtcgaaa atctcacgag cacgaatatc ttggatttga gccataacat
     5041 cctttctctt ttatgtcatg tcgagctagt cgacgaaaac tctcgtaaag ggatcgtcgc
     5101 tcagcccgag atgacattat tttttgacgg ttctgtgcta tcccgtattc acgggcagct
     5161 ccgaacgacc ctccagctgt cgggccaggt taataatacc aaaccttata gcaaaaaaga
     5221 actcccttgc ggtaaggaag ttctttcttc tttgttctaa agcgttgcgg cgactttatc
     5281 gagggcttct tcgcagttaa tccgaatgtt cggcagaagg tcaaacttcg gatcgttcaa
     5341 ctcttcacgg gtgaaccact taatctcgga gtgagcatct ttttctaagg taacttggtc
     5401 gctcaaaccg cgggcgtagt agccaagggc gatatgttta tgggtgtcac taatcgcgtg
     5461 aatatccatg taattcgggg ttggtaacat tcttccccca ctcactggta cttctgagcg
     5521 aggagaaagt aaatcaaact caattcccgc ttctttttgc acgattcgta agagtgcctg
     5581 ctcaggatct tcgtgcaatt caatatgacc gccaaccggg agccattttt gaagagcggt
     5641 atggtggact aataaaactc ggtccgtatg aacaataaag gctgtaacag tccagtcgat
     5701 caaatcatga atgtgtggca taaaaactcc ttactcgtag tatagcctga gtcaggaaaa
     5761 atcaaagagg gatacttaca aatgaatcag gtttatcgag ctaatactga ttcatacaca
     5821 tcaagcactt gttcgatcat tgctgccggc gtgcgctttg accgattcgt aatacagcct
     5881 tgcttaagag ttgcatggag cgtgggattc gtgagaattg agcggatgga ttgagaaagg
     5941 tcagccactg taggtgctgt gaccaggccg tccatgccat cagtaacaag ttcgcaggct
     6001 cctccaacat ttaccaccac gctgggtgtt cctgcgacct gggcctcggc agtaacaaga
     6061 ccttgcgtct ctgtggttga aggaaataca aataaatctc cggccgcata aaagggatct
     6121 aataactcac gaggttggcg gccagcaaag tggattctat cggcggcaac acttagagcg
     6181 gcttgctcac ggagtgactc ttcttcaggt ccgctgccta caaataaaaa gtgaaggttg
     6241 gtgtgttcgt ttgctaatgc atcaaacgct tgaagggcaa ggtcgaggtt cttctccttg
     6301 gcgagacgtc cgacatacag gagcaaaaaa tcctctgatt taatgccata acgacaacgg
     6361 atctcctgct gttttgcggg agatatgtct tccgacttcg ggaggggtac cccagtagga
     6421 attactcgga tcgatgcctc aacgcccatc tgacggagtg atgtttcggt ggctttagac
     6481 ggcacaatga tagtggcgca ttcggcaaat atatcgtagt accaatgtaa ccatgggcct
     6541 aatccgtact ttgcaatgcg atcgcttatt gaggtaggta atttaacata gtgtccatat
     6601 gtctcataaa gcgtatggtg gtgtgctacg agagggactt taaacttttt agcgattttg
     6661 gcacctacca tccccattaa gaatgggtga tgaacatgaa caagatcaag tccattattt
     6721 gcaaagtagt ggactaagtg ttttgtacgc caaggttgag caatggggta ctcccacaat
     6781 tccccaattg caggaattcg tagtacgtcg gcgggatcat tttgatttcc agcttctggt
     6841 gcgacaatcg ttgatttaaa attggagggt aaattttgaa caagcgtatc aacggcgacc
     6901 gtcactccat ttgtgagggg gtaaccttcg gtaaactgcc caacgtggcg atttttgatt
     6961 agagaaaaag taatagcact ctttgtaagt gcagtagtct gctgcatggg ctttatttta
     7021 caccaagaaa catacgtctg ggaagggatt ggcttactta gcaagaagca aaacggcccg
     7081 tggtgacgct tccgcgctcc actgacggcc tgagttctac acgatgtgtt ggggggtggg
     7141 tggtactgct cgctctgtca ttccggcgta ggctgagtcg tcgacttcgc ctaatcggtg
     7201 aagtgcttca acgagccccg ctggaggagg gacgacgata aggccgtagc aggtacggct
     7261 ggtgcaccgc acgtcattat ccactctctt tccgcaggcc cggtggcgta gattctcgta
     7321 ttcatctacg tagaggttgc gttcgaacca ctccaggtag gtttgagccg aacgagcttt
     7381 ctccatgggc cacaaacctc cagcaagtag caaaccaagt ttccgcttgg cctcagtgta
     7441 gtctgggacg ggaattggtc gttccgtccc ttctacaaca ataatacttc ctacccgacg
     7501 ttctctccca cggtggcgat acctcaccat agttgttatt ctttcttgcg cgcgcaacct
     7561 tagatggata aaaataagta tagttgttta atgcttttct gtcaagctaa ttcggcttcg
     7621 agctgagcaa tatagtcctc aatcattttc aagccgtttt gccaaaagtc gggggtgctg
     7681 acatcaaacc cagcaagtgc gaggttgtct ttcggggcca tcgacccacc gcggcgaaga
     7741 atgtcgaggt agttttcgac catctcgctc cccttctctt tataggtttt gtagatggcg
     7801 agggtgcaaa gctgagcaaa cgcgtatgaa tagacgtaga aaggccagtt aaacacgtgg
     7861 ggaatgtaca tccaaaagtt cttgtgttcg tctgtccaga ccaagctgtc gccaaatggc
     7921 ttcttaatga tttcatgcca taggtcgtcg atttgatctt tggaaagttc tccgtcggta
     7981 cggcgctttg tatgaacggc ttgttcaaac tgaaacatgg caatctgccg gtggacggta
     8041 gcgaagttat cttcaatctg tcccatgagc aaggcgattt tatcttcttt ggttaagtct
     8101 ccagctaaga gtttgtcgaa gaggaggagc tcagagaaag tactggcaat ctcggccaaa
     8161 gcgagggagg gatgcatttg gaagaagaca tttgtgtctt tgacaagtac gtcatgaacg
     8221 gcatggccga gttcatgcgc taaggtcatc acgtcgttca cgtcgtttgt gtagtttaag
     8281 agcacatagg ggtaagattc aggtgtccca aactgacaaa acgcaccacc ccgttttttg
     8341 ttgcttggaa accaatcaat gtgcgctttc tcgaacatac cgttggcgat ctcgcgaact
     8401 tgtggagaaa attcttcaaa tgctgcaaga accatttctt taccctcttc gggggaaatc
     8461 tctttctttg gttttggtaa cggggcatag cgatcccacc agtgcagcgt atcttcgcca
     8521 aggattttct ttttaagggt atagtagcgt tcaactaagt ggctgctctt gttgacagct
     8581 tcgattaagg cgtagacagt atctttctcg acgtcgtcac tattaaaccg tcgctcttca
     8641 gggtaatcat agttgcgaag tcggtcgctt atttctttat cttgaatgag ggcgttaaaa
     8701 accgctggag tcgttttgct atttgtgtta agtgcggcat gaatggcaaa gacggcttgt
     8761 tttcgaattg ctttatctgg atcgcgtaaa tatggtgaaa tttcttcgag cggttttttc
     8821 tctccgttcc agtcaaccat taaggtgtcg gtggtaatac taaataaatg gagtagcgct
     8881 tggctactgg tctggctttt ttcggccagg attttttctt ctggctcaga tagcgtatgt
     8941 tttgcttctc gatacgtgtc ttctaaagtc tttttatatg cgttaagttc aggagcgttt
     9001 aaaaattcct gccatttggg ttcgggcagt tgcgccattt ctacttcaat aaaaatagtc
     9061 tctttgccaa gctgaatcat acgggcgtcg agttgttttt caaaacgggt gacttcgggc
     9121 acttcgacat tggtagccga aagcaggctg gcgtacacgc ccaggcggtt acttttaaga
     9181 gcgagagctt cttggctttt tataagggta agcatttcgc taggattgag ggtggcgatc
     9241 tttcctcggt agtcctgtag ttcacgtacg agggcttcag cggccgaaaa atcttcttca
     9301 attttgggat cgtccatccc gctgtagaga tcgttaaggt tccaggttgg ggtaattgat
     9361 tgctgtgtca tagcgtaagt atagcatagt acctgaagtg ctgattcata ttaagagcga
     9421 acaaacactg gaaaaaagga cgaaactttg ggatactcta ccaaatcact ctctgcagat
     9481 gtctactcaa ggagcgggta gaagcatggt tgcagcactc tggattgcaa cgatcggcat
     9541 aattgccgac catgttttta tagggatgga gctgcggagg tgtcacgcgc gatttaaccg
     9601 aacgcagttt tggtttgaaa tcgtgaggac gcactcttgt tttctccatt actatccgta
     9661 tctctctgac ttatgggaga gagacggtaa gcctgaatta ccgtaatcgt tggcaccggg
     9721 gtcgagcagt tgctcggccc ctctgtttat atacaaaggc ttgacaaaat agctattttg
     9781 tggtatataa ctatttagat agtgagtacc gactttggga gacttttcga tgccataccc
     9841 tgttaaggcc gttgttgctt ttgccctttt cttcgtcctg ttgctgggcg atctcctgtc
     9901 tgccctactg cattgggtgg atccgacggc actcaaccgc tggggcgctc tggacgggct
     9961 gctccgtcag tgtgaggaca cctacaacgg gctcgtggag tccatccggc cttcgccgag
    10021 caagtccgca caggtcgtgg cctgtgctcc gtcgctcgac aaggtagagg taccaaccga
    10081 gcctatcagc ttcgtggagt tctaatccac gtagcgcccg caatgaacgc ggccgaggag
    10141 ctttgctccc cggtcgcgtt ttacgtttat taaagataaa taatagactt gcttaatact
    10201 gtaggtgtgt tactgtccaa gagttgactg acatggagag gagggctttt acgtaatggc
    10261 tacgaaaaca atttttatta ctggtggaat cgtaagcgga cttggaaaag gaattacagc
    10321 cgcgagctta ggagccctgc tcaaagcccg cggtttttct gttataccga tgaaaacaga
    10381 tatgtatttg aatgtcgacg ccggtactat gaaccccttc cagcacggcg aagtatttgt
    10441 gaccgaagat ggtgcagaga cagatttgga tctcggccat tatgaacgct ttttagatag
    10501 tgatttaagt aggctttcct attcaacgat gggatcagtg tttcaagcgg taactgaaca
    10561 cgaaagaaag ggaacgtatt tgggcaagac catccaaatc gtaccgcatg ttaccgatga
    10621 aattcagcga cgcttccaaa ctatctctga gacagctcag cccgattttc aaattattga
    10681 actgggaggt acggtaggag actttgaggc tgaggttacg ttagaggctg ttaggcagtt
    10741 ccaaactcga aacaaacgtg attgcatttc tattcatgtt gtaaagatgg attacattta
    10801 tccttccgat gaagccaaga cgaagccaat gcagcattca gttattgcgt tacgtcgcta
    10861 tgggattaac ccagatattc tcgtggttcg tgcaaagcgg ccaattagtc aggatgaaaa
    10921 agaaaaaata gcgctctttt gcggtattga ttcagaccat attatctccg cattagatgt
    10981 ggagtctctc tacgatattc cgcttaacat ggaaaaagaa ggtctggcac gggtgacatt
    11041 gtccctccta ggtataaaag agagtgctcc taatctcgct gagttagaag agattacccg
    11101 ccgtaccaaa cttacagaac ccgttattcg agttgggtta gttggaaagt atacagatct
    11161 ttccgatgcg tacctctcgg ttatagaggc tgcaaaacac ggaggtatcg ccaactcggt
    11221 gaaagttcaa gtcgtgccag tggactcgga gcatctgaat gtacagttat tgaaggaagt
    11281 tgacgccatt attgtgccag gtggatttgg tactcgtggt gtcgaaggga agattaaagc
    11341 gatacagtat gcgcgcgaaa acaaagtccc ctatctcggt atctgtcttg gcttacagac
    11401 ggctgttatt gaagcagctc ggaatctagc cgggataaaa gacgcgacaa gcgaagagtt
    11461 tgacgagaaa gcaaatcacc ctgttattgc ctttttacca gatcaaaaag gtattactcg
    11521 caaaggtggt acaatgcggc tcggctcata cgaagcagtg atggcagact ctctcatttc
    11581 tcgcctctat gcttggtggg aagataaaaa agcgtttgac ttcccctctc tcgatgaggt
    11641 gatgaaattt aaacctgtta aaacatcatt ggagcgtcac cgtcaccgct atgaagtgaa
    11701 tccagactac cataaagttc ttcaggaagc tgggttgcgt ttttctggca tgtcacccga
    11761 cggctcgctc gtagaattca tcgaatatcc acgagaagtt catccttact ttgtggccac
    11821 gcaggctcat ccagaattta agagtcgacc ttataaagca catcctctct ttgccggttt
    11881 gatcgccgcc gccaaagaac agagtcagaa tcacactact ctagcgcaga tgccagtcga
    11941 ttctgttcaa acagttgcct catgactttt ttgcaaagag tgatatgcta actgggagtt
    12001 tatctatctt cgaaataagg aatgtgaaat atgtctcacg tctcgcataa ctacgctgtt
    12061 attattgctg gtggaacagg tgctcgcttg tggcccgtca gtcgcaaatc aaagcctaag
    12121 cagtttcatc ctctactagg ggaaacgtcc ttgttccaag ggacatataa gtcactctcc
    12181 aattgtctcc cgacgagtaa tattattatc caggttaatc aagaattaat tccgttagta
    12241 aaagagcaaa ttcctggtat cgacgagagt aacattatat ttgagccagt gggacgagat
    12301 actggtccag cctacctctt tgccgccgct acggtcgcac aacgagatcc cgaagcgaat
    12361 gtcgctatct tttggtcgga ccatattatt aagaaccaag ctaactttaa gtcagctctc
    12421 gaaaccgtgt ttactaccct taatgaaaat cctgaacgga ttgtttctct tggggtgaag
    12481 cccgacttcc cgcatacggg attgggctac attgcattag gacaaaagtt taaagagcaa
    12541 cctacgggaa cggtttttga agtggaacgt tttgtagaaa aaccggctta cgacaaagcg
    12601 gtggaatatc tcgcaaccgg aaactattta tggaataccg ggtatagcgc cttctccgtg
    12661 gctggatttg aatcggttct ccagagtgcc gacccaacac tcgccactca ctattcagaa
    12721 atgaagcatt tgatagagca aaacaatgtc gaatctttac gcgaattttt cgagtcattg
    12781 ccacgcattt cgattgatta ttggctcatg gaaaaattaa ccaacatgct tgccgtgcct
    12841 gcggaactag aatggtcgga tgttagtaat tggaaaacag tttatgatct cttgagtgag
    12901 gccgaaggga ctgatatggt tacccgtggt gatcatatct cttatgactc gcataattgt
    12961 ttagttatag ggaataagcg gttagtggta accgctggag tcgagaacct cattctcatc
    13021 gacacgggcg acgtcgtttt tgctttaaat caaaactctg ttcatgaaat gaaagaagtc
    13081 gttgcgttaa tcaaagaatc aaaatttagc gacttacttt aacggctcag agtctatttt
    13141 gcacctcttg ggtacatatg gtatgttggg cgcgagatag tttttttgag tatgatttta
    13201 taatgtctct tgatgcacta taggtaaaat gccatgtcac cactcgataa tcgcgcgatt
    13261 ataaagcagc tagataccga caactactta agccatctct tagatgcgcc ggaacagatt
    13321 gttgatgggt ttcatttagg tgaagaaatt acaatcccag ctttatatgc gcaggctaaa
    13381 caagttgtgc tcttggctac gggggaaatg ttgccggttg ccttggcagt gcaagctctc
    13441 gctgttgagt atgctcgcgt tccggtggtg attgtctttg attatgtttt accccattgg
    13501 gtttcgagtg atactttggt aattgccctg gattattatg gtactaccga gcaagtatta
    13561 gtcgcctttc gcgaagctgc ttcacgtcgt gcccgattac tggcaatctc ggtgggaggc
    13621 gatttatcgc gtgaagcccg ccgagttcgg gctgtgcatg tgccattaag ctacggcgct
    13681 cctgctcggg cagcattttt ctatgcattt agttgtgttg cctatatttt tcgcaagctc
    13741 gatcttacgg aattgcgaga gtcaacggtt acagaatcat cggttcttac ccgttccctt
    13801 ttagaaagca ttggcccgga agtcgctcaa taccagaata acgccaaaca gctggctgag
    13861 aagattctta cccgtaaaac aattgttgtt gggagtgggc ctttgattgg tatcgcgaaa
    13921 cgttggcaat tggctcttgc tcaaaacagt aaagccgtta tcacgtctag caccttgaac
    13981 gaatttaatg atacgattat taatggatta ggatattttt caaagccgat tgaagcgccc
    14041 tttgtgataa tgttgcagtc gaagtacgac caccctcgca ataaactgca gcaaacactc
    14101 acgtatcagg ttgcccaggc tcagaaaatt atttacgagc agatctttat gcacccgagt
    14161 ggatcgctgt ttggagaaat tgtgctcgca gctcttctgg gtgacatgac aagctactat
    14221 gtggcgcttc tcagtcacca taatccttct ccgctcgatg cgaccgacta tatccgaggc
    14281 cgtctaaaag aagaaaaatt atttgaatag tccacaccca tttgggtgaa ggagggacat
    14341 ggcagttatt gcttcggtgg cagctcgagt tattaacgac tcgcgaggtc aaccaactat
    14401 cgaagttacc gttaccgacg acaaaggcag aatcgcaact gcctcgcttc cgagtggtag
    14461 ttcccttgga aaatatgaag ccgtaacaca agatgcgcag caagttgtaa actctatcaa
    14521 caaagtaatc gcccccgctc ttctgcggca gccgttaacc gaccaagcca ctatcgacgc
    14581 caaacttcgt tcttttgacg atacggctaa tcacgagaag ctcggtgtaa acagcctctt
    14641 aggcatttcg ctcgcctgtg ctcgtttatt agcgcaggat cataatcttt ctctctatca
    14701 ttatattttt actatctctg gcggaccggg actaacaatt cccaccccca tgtttaactt
    14761 gcttaacggc ggaaagcacg ccgagaataa tctcgacttc caggaataca tggttattcc
    14821 gatcggtttt aaaacctttc acgaaaaact cgctgctggt aaaaaagttt ttagcgcatt
    14881 aggaaccatt ctttccgaga caggacatat tacgaagata ggagccgaag gtgggtatgc
    14941 acctcatttg tcgaccaacg aagaaggcct gggtgttctg gtacaggcta ttcagtcggc
    15001 cggctacgaa cctgggaagg attttgtact tggggtagat gtggctgcct ctgcattgcc
    15061 tcctacgtat tctgcgactg tcgataacta tctggcgctt taccgaaact ttcctctctt
    15121 ctccctggaa gatccgtttg tagaagatga ctgggagagt tgggccaaat taaaagtcgg
    15181 agtcgatggc ttaagcgaac caggtaatcc tcgattgctc gttggtgacg atctttttgt
    15241 cggcaacaag gaccgtttac tcgagggggt taaaaagttt agtgccaacg ccgtacttat
    15301 taaaattaac caagccggca cgttaacaga aattatcgag gcaattaaaa ttgcccgcga
    15361 acataactac gtccacattc tctcccatcg ttctggcgaa acgctcgatt cattcgtaag
    15421 tgatttagca atcggtactg ccgccgcctt tattaaagct ggttctgcca acgaacaagc
    15481 acccgagcga atcgtaaaat atgaacgtct cgtcgaagtc gaagaagaac tgaatattgg
    15541 tcatatatga aagcaactca cattgcagtt attacccatc cccgcgctca caactcttta
    15601 gccaaacggt tagagaaaac ctttcgagtc ggtaagcttg atggtattta taaactgcct
    15661 tactcacact cccctctctc gcctgctggt acgtcgccgt attcagtgct ctattcgctt
    15721 tcacatcctt caaaagaact aacccatctc aaaaaattag aacctgtcca cgtcagacag
    15781 ttacgcgata ttgcttatag atgtcgtaag tcatcgggaa ccctgcatat tattggtgtt
    15841 ctggatgatg actccccgtt tggaaaacgg cggatggtag aagcgctcgt acgtcatttt
    15901 cggccaacgg gactcaaact tgtcttgcac cttggcgtct ggcatccaac tcctacggaa
    15961 tttcggtatg ggttaaaaga aattcggagc ctatgtaatg aatcta
//
LOCUS       DASWYB010000007         8046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_723093, whole genome shotgun sequence.
ACCESSION   DASWYB010000007 DASWYB010000000
VERSION     DASWYB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723093"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..913)
                     /locus_tag="VGE59_00425"
     CDS             complement(101..913)
                     /locus_tag="VGE59_00425"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY1074155.1"
                     /translation="MKDLLTRYARSAYTVFKQLKLKGLITAFSSGAEFSQLIKPLGSR
                     PLILSGHPGDEVMAMGGTMAWYGKLKAPVAVVTFTAGRRGTNTGRMSKSLGPKRRKEQ
                     IAAFGVLPVEVHPTYWDLDERFPVTEDLIFQLLETVDEINPDIIYVPSLLDIHPDSQA
                     LNRALVSVIERLPSPRLKNLQIAQYELWTPIVPNKILSLDGYEETKQKAIECHESQLL
                     CRNYLDAMLGLNRYRAAMLGAGNYAEAFFVCSAKQYVEFLNIRPAPVIEMLP"
     gene            complement(910..2376)
                     /gene="gltX"
                     /locus_tag="VGE59_00430"
     CDS             complement(910..2376)
                     /gene="gltX"
                     /locus_tag="VGE59_00430"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY1074156.1"
                     /translation="MSKPIRVRIAPSPTGDWHLGNARTALFTYLFARHHGGEFFLRVE
                     DTDQGRLVEGAVDRLLVLMEWLGLTPDLYDGLPYVRQSDRLDLYKEAAEKLIAEGKAY
                     YCFATGEELAHMRSEQEAAKLPPRYDNRWGYRDLPLEQAQARIAAGDSYVIRFKMPQE
                     GETVVVDLIRGEVRFANNLTDDFVMIKADGFPTYHLAHVVDDHLMEISHVTRGDEWLS
                     SVPKHVQLHEALGWDLPEYIHFPVILGPDKGKLSKRHGAKPVFEYEIDGYLPEALINF
                     LALIGWSSGTDEELFSLDELIKKFDVKGLQKSPAVFDATRLDWFNGQYIRRLSSRELS
                     SRLYEYYDVRKDPVILERFDEYSEKMPAIIASVHERLVTLKDFAEHTYFYFVAPEAYD
                     PNLLPAKKQTLEDVSRVLEISVSALSSLEDWSHDSLEQYLRQVGVEHDLKVGDLLWPL
                     RVALTGLPASPSTFDVLAILGKDESLKRIAYAQALLAS"
     gene            complement(2386..2736)
                     /locus_tag="VGE59_00435"
     CDS             complement(2386..2736)
                     /locus_tag="VGE59_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY1074157.1"
                     /translation="MNTRSVGNSGEEAAVLFIEQLGWQVVGRNLHYRFGEIDILATNG
                     THMIVIEVKAKKSSMYGEAVEMVTRSKQATLRKLAKVVEAEYNKPVRIDVITLDNFAT
                     SQASVTHYPFAIGE"
     gene            complement(2801..3355)
                     /gene="rplS"
                     /locus_tag="VGE59_00440"
     CDS             complement(2801..3355)
                     /gene="rplS"
                     /locus_tag="VGE59_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY1074158.1"
                     /translation="MDAIKHFTLSSLKRSVPELRPGDTVRVHQKIREGNKERVQIFEG
                     VVMAVRGGKAINSSFVVRRIASGVGVERTFPLHSPNVVKVERVKSGKVRQARLYYLRE
                     RFGRAARLAGEVKSTDQWHEGQVEDEVVAEVEEPAMESPEESTEAEQSVETEEPTEVE
                     APAEEVAPTEEAADAESEAAENKE"
     gene            complement(3487..4011)
                     /locus_tag="VGE59_00445"
     CDS             complement(3487..4011)
                     /locus_tag="VGE59_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074159.1"
                     /translation="MAVVGTSNSAQAPAEKQPLPGLLGNMAGHDEEFVSSAAQVTVGP
                     VKIVGSDVYFDGMPIRGERITEASLTAKPEGELRKMLPEQLVLLLVTLLFDEDGNELA
                     SAYALRVEYQQYEFLIIEKSKPEEQAASLQGVLLRFELGGLRDYALTRELRARQSRIE
                     HGDAIYEAYQEMSH"
     gene            complement(4033..4317)
                     /locus_tag="VGE59_00450"
     CDS             complement(4033..4317)
                     /locus_tag="VGE59_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074160.1"
                     /translation="MVTCGADDEELLSEGPIGELQISSMAAVVAIGANGKAKVTPPPG
                     FRFYMEGSVLMVRPAVPEEEGIAEEKVLCSRNERVLRVFRLPFFRSRVVS"
     gene            complement(4421..4702)
                     /gene="rpmA"
                     /locus_tag="VGE59_00455"
     CDS             complement(4421..4702)
                     /gene="rpmA"
                     /locus_tag="VGE59_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY1074161.1"
                     /translation="MAHTKGKGSTKNLRDSVAKRLGVKTFGSQTVHAGDILVRQRGSK
                     FYAGENVGRGGDDTLFAKIGGVVRFQVKRVRNFNNQLREKRVVHVDTEV"
     gene            4912..>8046
                     /locus_tag="VGE59_00460"
     CDS             4912..>8046
                     /locus_tag="VGE59_00460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY1074162.1"
                     /translation="MSQFVHLHVHSHYSLLDGLGKIDELIARAKELEMPALALTDHGN
                     MHGAVEFYTKCKKAGIKPIIGVEAYLSPRGMLDRVPKVDSTAYHQVLLAKDDVGYRNL
                     LFLVSEAHTKGMYYKPRIDLPLLAAHSKGIIATSSCLAGMIPEAIMAGDMEKAIALTR
                     QFVDMFEGDFYLEMQHHPSLDKQMRVNEGLKAIAKELSLPLIATSDVHYVRKEDKDTH
                     EVLLAINTGKDLASDDRMSLADVDLFMHDEEFFKEVFPDTPEIIENTAKIAEKCDLKL
                     TLGENIIPDFDCPEGFTPDTYLEKLCNEGLRKRYGDAITDVHTERLSFELATINKMGF
                     ASYFLIVADYVNWAKSQNIYVGPGRGSAAGSIVSYSLGITDMDPLPFGFLFERFLNPD
                     RISMPDIDMDFADDDRDKVIGYVRQKYGEDHVAGMATFGTMMGRAAVRDVGRVLGMTY
                     AEVDEIAKLVPPPVQGKHIPLKLSIQNVPELKQRYEADPRMKKLLDFARKLEGTVRHA
                     SQHACAFVICKEPTVYYTPVQPAQRGREGVVTQYSMKPIEEIGLLKMDFLGLSNLTIM
                     RNAVRIIRKVYGAEIDIHNLPMDDKKTYELLSRGETTGVFQLESDGMKRYIKELKPTE
                     FNDIIAMCALYRPGPMANIPTYIARKHGKEPVQYLHPKLANAFAKTYGIAVYQEQVMQ
                     ASKDLAGFTGGEADTLRKAIGKKIPALMAEMKSKFVEGCKKHSDIDEDLADKIWKDFE
                     EFAAYGFVLAHAGCYALIAYQTAYLKANYPAAFFAALMTSNADNLDKMSIELEEAKTM
                     GLQVLPPDINESFPEFGVVRGEGKEYIRFGLGAIKNVGLGVAESIVEERTINGPYTSL
                     TNMLTRLGPSVLNKRVLEALGMTGAFDRWGERNALVQSVDTMTKFLSNGKKDTNQVGL
                     FGAETPSLVRDIVLMDVPPAEQRIYLEWEKMLLGIYLSGHPLKAIEHIVPQIATKIAT
                     ISVAQEGQNQRVAGMIMDLRAINTKTGDQMAFAKLEDPTGTLELVIFPRTWAEAKIFC
                     QKDRYIVADGRVDTR"
BASE COUNT         2112 a         2147 c         1868 g         1919 t
ORIGIN      
        1 agggcataaa tctaatgtac agcggttcta aaaagacaac aataccagaa ccctaccaaa
       61 ctcttgacca ttttacctga aatagagagt tttgtgaagc ttaggggagc atttcgatca
      121 caggggcagg gcgaatgttc aaaaattcta catactgctt tgctgagcaa acaaagaacg
      181 cttcggcata gtttccagcc cccagcatgg ccgctcggta acgatttaag cccagcatgg
      241 cgtctaagta gttgcggcat aaaagctggc tctcgtgaca ttctatggct ttttgctttg
      301 tttcttcata cccatcaagg ctcaaaattt tattggggac aattggcgtc cataattcat
      361 actgcgcaat ctgtaagttt ttcaggcgag gagaggggag ccgctcaata acagaaacca
      421 aagcccggtt taaagcctgg ctatcagggt gaatatctag gagtgacggc acataaataa
      481 tgtctgggtt aatttcgtct accgtctcta gaagctgaaa aataaggtct tccgtaacgg
      541 gaaagcgttc atccaagtcc caatacgttg gatgaacctc caccggtaat acgccaaaag
      601 cagcgatttg ctccttgcgt cgctttgggc cgagcgattt gctcattcgt cccgtattgg
      661 tgcctcgtcg accggcagta aacgttacga ctgccacagg agctttgagc ttgccgtacc
      721 aggccattgt gccacccata gccataactt catctcccgg gtggccagaa agaataagtg
      781 ggcgagaccc aagcggctta atgagctgag agaattccgc cccgcttgaa aatgcggtaa
      841 tcaaaccttt tagtttaagc tgcttaaaaa ccgtgtacgc gctacgggcg taacgggtta
      901 gtaaatcttt catgaggcaa gtaacgcctg agcgtaagca attcgtttaa gagattcatc
      961 tttccccaga attgccaaga catcaaacgt actgggagaa gcggggagtc ccgttaatgc
     1021 cacccggaga ggccagagca aatcaccgac tttaaggtca tgctctactc caacctggcg
     1081 aagatattgt tcaagcgaat cgtgcgacca atcttcaagc gaagaaagag ccgatacact
     1141 tatctcgagc actcgggaaa catcttcgag cgtctgtttt ttagcaggta acaggttggg
     1201 gtcgtacgcc tcaggagcca cgaagtagaa ataggtatgc tcagcaaagt cctttaaggt
     1261 gacgagtcgc tcatggactg aagcaataat cgcgggcatt ttttctgagt attcatcgaa
     1321 ccgttcgagt ataaccggat cttttcgaac atcgtagtac tcgtaaagcc gactcgagag
     1381 ttctcgagaa gacagacggc ggatatactg tccattaaac cagtcaagcc gggttgcatc
     1441 aaaaacggcc gggctctttt gcagcccttt gacatcaaat ttcttaatta attcatcgag
     1501 agagaagagt tcttcatccg taccactcga ccaaccaatg agtgccaaga agttaatcaa
     1561 ggcttctggc aaatatccat caatttcgta ttcaaatacc ggcttagcac cgtgacgttt
     1621 cgagagcttc cctttatccg gacccagaat aaccggaaaa tggatatatt caggcagatc
     1681 ccaccctaag gcctcatgga gctgaacatg cttgggcact gaagagagcc actcatcgcc
     1741 tcgagttacg tgactaattt ccattaggtg gtcatctacc acgtgcgcta aatgataggt
     1801 tgggaaaccg tcagctttga tcattacaaa atcgtcggtg agattattgg caaatcggac
     1861 ctcaccacga atgagatcaa cgactaccgt ttcaccttct tgtggcatct taaaacggat
     1921 tacataggaa tcacccgccg caattcgcgc ctgcgcttgc tcgagcggca gatcacgata
     1981 cccccaccgg ttatcatagc gaggaggaag cttggccgcc tcttgttcgc tgcgcatatg
     2041 cgctaactct tcacccgttg caaaacaata gtaagctttg ccctcggcga taagtttttc
     2101 cgccgcctcc ttatagagat cgaggcggtc cgattgacgg acatagggca agccgtcgta
     2161 caaatcaggt gtcaagccga gccactccat taaaaccaag agccgatcta ccgctccttc
     2221 taccaggcga ccttgatcag tgtcttcgac gcgtaagaaa aattccccgc catggtgacg
     2281 ggcaaaaaga tacgtaaaaa gggccgtacg ggcgttaccc aaatgccaat caccagttgg
     2341 acttggggca attcggactc tgattggttt tgacatactg actcctcact ctccaatagc
     2401 aaacgggtaa tgggttacgc tcgcttggct ggtggcaaag ttatcgagcg taattacatc
     2461 aatccgaaca ggtttattat actctgcctc gaccactttg gccaacttgc gtagcgtagc
     2521 ttgtttcgac cgggtcacca tctctactgc ctcgccatac atcgacgatt ttttcgcttt
     2581 tacttcaatc acaatcatat gagtgccatt ggttgccaag atatcgatct caccgaaccg
     2641 atagtgcaaa ttccgcccaa ctacctgcca ccccaactgc tcgataaata aaacagcggc
     2701 ttcttcccca ctatttccaa cggaccgagt attcataccc ctatcataca caaatcccgc
     2761 cagtgaggcg ggacttaatg cattcgttta tgaataaaag ttattcttta ttttcagctg
     2821 cttcagattc agcatctgca gcttcttcgg ttggagcaac ttcttctgcg ggagcttcaa
     2881 cttctgttgg ctcctctgtt tcaacagatt gctcagcttc ggttgattct tcaggagatt
     2941 ccattgctgg ttcttcaact tcggcaacaa cttcgtcttc gacttggcct tcatgccact
     3001 gatcggtgct ctttacttcg ccagcgaggc gagcagcgcg cccaaagcgt tcgcggaggt
     3061 agtaaagacg agcctggcgg actttaccgc ttttcacacg ctcaacctta actacgtttg
     3121 gcgaatgaag agggaatgta cgttcaacac caacgccaga ggcaatgcga cgaaccacaa
     3181 aactggagtt aatagcctta ccaccgcgaa cagccataac gacaccttcg aaaatctgaa
     3241 cgcgttcttt gtttccttca cgaattttct ggtgaacacg aacggtgtcc ccagggcgaa
     3301 gttccggaac actgcgcttt aatgatgaga gagtgaaatg tttgattgca tccatagcaa
     3361 attccttcaa ggagatatat tactcaagaa ttataacttt ttttattcaa agaagcaaga
     3421 gacaaaaagg ggccagcgcg gaagcgtccg cgcaagcccc ttctaaagcc tagcacccca
     3481 ggagaatcag tggctcattt cctggtaggc ctcgtatatg gcgtccccgt gctcgatacg
     3541 ggactgccgt gcccgaagct cacgcgtcaa cgcataatct cggagacctc ccagttcgaa
     3601 acgaagcagc accccctgga gggaggccgc ctgttcttcc ggcttcgact tctcgatgat
     3661 caggaattca tactgctggt actccaccct cagcgcgtag gcagaggcaa gctcgttgcc
     3721 gtcctcgtcg aagaggaggg ttacaagcaa gagcacgagc tgctccggaa gcatcttgcg
     3781 caactcgcct tccggcttcg ctgtcaaaga cgcttccgta atacgctcac cgcgaatggg
     3841 catgccgtca aagtagacgt cgcttccgac tatcttgaca ggccccacgg ttacctgcgc
     3901 tgcactactg acgaattcct cgtcatgtcc cgccatgttt cccaggagac ctgggagagg
     3961 ttgcttttct gctggcgctt gcgccgagtt cgatgtgcct actacggcca ttttcaggtt
     4021 ccttacggtt ggttacgata ctacacgtga acggaagaag gggaggcgga agactcgaag
     4081 cactcgttca ttgcgtgaac atagcacctt ctcttcggcg attccttctt cttccggcac
     4141 agccgggcgc accatcagga cgctcccctc catgtagaat ctaaatcccg gaggaggagt
     4201 tacctttgct ttaccgtttg cgccgatagc cactacggca gccatgctgc tgatctgaag
     4261 ttccccaatg ggtccttcac taagcagctc ttcatcatca gccccacacg ttaccacgag
     4321 tctcaccccc ttctcatgcg cgtggttcgg acatactacg tccgaagagg ccaaagtgcc
     4381 tcagagtaca agcgaagtta ttcgcttgca cgagagatac ttaaacctcg gtatcaacgt
     4441 gaacaacacg cttctcgcga agctggttgt taaagttgcg aactcgtttt acctggaaac
     4501 gaacaacgcc accaattttg gcaaaaagag tgtcgtcgcc tccacggccg acgttctcac
     4561 ccgcataaaa tttgctgccg cgctgacgca ctaaaatatc gccagcgtga actgtctgac
     4621 taccaaatgt tttaacgccc aagcgttttg caacgctgtc gcgtaagttt tttgtcgagc
     4681 ctttaccttt tgtatgtgcc atatcacctc tttttcgtgt tagaagagat aagttgttgt
     4741 acttatccct agtgccaagc cttttaaata gtggttacag tatacatgga ttcatcactt
     4801 tgagcaagtc ccataaccga gcaaacacgc ctgtgcgatt atcaggcttg gagataagcg
     4861 gcaccgtagg gtatgctgag ggacgagtta gaaggaaggg aagatctgcc tatgtcacag
     4921 tttgttcatc tgcacgtcca ctcccattac agccttcttg atggcttggg gaaaatcgat
     4981 gaactgattg cccgcgccaa agagctcgag atgcccgctc ttgcattaac cgaccacggc
     5041 aatatgcacg gcgcggttga gttttatact aagtgtaaaa aagctggcat caaacctatt
     5101 attggggtcg aagcttacct ttccccccgt ggcatgctcg acagagtgcc caaagtcgac
     5161 tcgacggctt accaccaagt cctcctcgcc aaagacgacg tcggctaccg aaatctcctc
     5221 tttttagttt ccgaagccca caccaaaggc atgtattaca aaccccgcat cgacctgcca
     5281 cttctggcag cgcactcgaa agggattatc gccacctcca gctgtttagc aggtatgatc
     5341 cccgaagcca ttatggccgg cgacatggaa aaggcgattg ccctcaccag gcaatttgtc
     5401 gacatgttcg aaggggactt ttacttagaa atgcaacacc acccgtcact cgacaagcaa
     5461 atgcgggtca acgaaggcct caaagccatc gccaaagaac tgtcactccc acttatcgcc
     5521 accagcgatg tgcactacgt ccgtaaggag gacaaggaca cgcacgaagt gttactcgcg
     5581 attaacacgg gcaaagattt ggcctccgac gaccgtatga gtttggccga tgttgacctt
     5641 tttatgcacg atgaagagtt ctttaaagaa gtttttcccg acactcctga gattatagaa
     5701 aatacagcca aaatcgccga aaaatgcgat ctcaaactga ctcttggcga gaacattatt
     5761 cccgacttcg actgccccga agggttcacc ccagacacat acctagaaaa actctgtaac
     5821 gaggggttgc gcaaacggta tggcgatgcc attaccgatg ttcacaccga gcgactctcc
     5881 tttgagctgg caacgattaa caagatgggc tttgcctcat acttcttgat tgtggccgac
     5941 tacgtcaact gggcgaagag tcaaaacatt tacgttggtc ctggccgtgg ttcggctgcc
     6001 ggaagcattg tgtcttactc acttggtatt accgacatgg atcctttgcc ctttggattc
     6061 ctgtttgaac gcttcttaaa cccagaccgt atttctatgc cagatatcga tatggacttt
     6121 gccgatgacg accgagacaa agtcattgga tacgtgagac aaaaatacgg cgaagatcac
     6181 gtcgctggca tggctacctt tggaaccatg atgggccgag ctgccgtacg tgacgtaggc
     6241 cgcgttctcg gtatgacgta cgccgaagtc gatgaaattg ccaagctggt cccaccgcct
     6301 gtgcaaggta aacatatccc cctgaagctc tctattcaaa acgtacccga actaaaacag
     6361 cgctacgaag ccgatcctcg gatgaaaaag ctgcttgatt ttgctcggaa gctcgaaggc
     6421 acggtccgcc atgcttcaca gcacgcctgt gcctttgtga tctgtaaaga accaaccgtg
     6481 tactacactc ccgtccagcc agctcaacgc ggccgagaag gcgtggtaac gcagtattcc
     6541 atgaaaccaa tcgaagaaat tggcctcctc aaaatggact tcttgggtct ttctaacctc
     6601 acaatcatgc ggaacgccgt ccggatcatt cgcaaagtgt atggcgcaga aatcgacatc
     6661 cataacctac ctatggacga taagaaaacc tatgaactcc tttctcgagg agaaacaacc
     6721 ggcgtcttcc agcttgaatc agacggcatg aagcgctaca tcaaagaact caagccaact
     6781 gaatttaacg atattattgc catgtgtgcc ctgtaccgcc cgggtccaat ggccaatatc
     6841 cccacctata tcgcccgcaa acacggcaaa gaacccgtgc agtaccttca tccgaaactc
     6901 gccaatgcct ttgccaaaac ctatggtatt gccgtgtatc aggaacaggt catgcaagcg
     6961 tctaaggact tagccggttt tacgggtgga gaagccgaca cactgcgtaa agcaattgga
     7021 aaaaagatcc ctgccctgat ggccgaaatg aaatcaaaat tcgttgaagg ctgtaaaaag
     7081 cactccgaca tcgacgaaga cttagccgat aaaatctgga aagattttga agaattcgcg
     7141 gcgtatggat tcgttctagc ccacgccggc tgttacgcct taatcgccta tcaaaccgct
     7201 tacctcaaag ccaactaccc agctgctttc tttgccgcgc tcatgaccag caacgccgac
     7261 aacctcgata agatgtcgat cgagttggaa gaagctaaaa ccatgggcct tcaagttctt
     7321 ccaccagaca tcaacgaatc ttttccagaa tttggcgtcg tacgtggaga aggcaaggaa
     7381 tacatccgtt ttggactcgg agccataaag aatgtaggtc tcggagttgc cgaatcgatt
     7441 gtcgaggagc gcactattaa cggtccttat accagcctca ccaacatgct tacccggtta
     7501 ggaccaagcg ttttaaataa gcgtgtactc gaagcactag ggatgacggg cgctttcgac
     7561 cgttggggag aacgcaacgc tttagttcag tccgttgaca ccatgactaa attcttatcc
     7621 aacggcaaaa aagacacgaa ccaggttggt ctctttggcg ccgaaacacc aagcttggta
     7681 cgcgacattg tcctcatgga cgttccgcca gccgagcagc gcatctatct cgaatgggaa
     7741 aagatgctgc tggggattta cttaagtggc cacccattaa aagcgattga gcacatcgtg
     7801 ccgcagatcg caacgaagat tgcaaccatc tcagtggcac aagaagggca aaatcaacgt
     7861 gttgcgggta tgattatgga tctgcgagcc attaacacaa aaacaggcga ccaaatggca
     7921 tttgccaagc tcgaagaccc aaccggcacc ctcgaacttg ttattttccc ccgcacgtgg
     7981 gcggaagcca agattttctg ccagaaagac cgctacatcg tggccgacgg ccgggtcgat
     8041 acccga
//
LOCUS       DASWYB010000008         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_162741, whole genome shotgun sequence.
ACCESSION   DASWYB010000008 DASWYB010000000
VERSION     DASWYB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162741"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VGE59_00465"
     CDS             <1..486
                     /locus_tag="VGE59_00465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY1074163.1"
                     /translation="LQAQFQNRTTKKVYNALVFGEPKEHSGTIETFIGRHPKRRQEQA
                     VLPIQVGERARREAITDYIVKAVYFYHGQPISLIEFSPKTGRMHQLRVHAKYIGTPIL
                     GDQTYTIKPAKRMSKEVGLTRQLLHAYRLTIEHPETHSPISLEAPLPDDMVNLLKLID
                     S"
     gene            545..1123
                     /locus_tag="VGE59_00470"
     CDS             545..1123
                     /locus_tag="VGE59_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074164.1"
                     /translation="MGHTYPPIIIVSGAVGSGKGTVIHALAHELGLEWVPTHTTRAHR
                     PDDETLSRHVFDTEPTFLRHLARNEYLETIEYKGERYGLLREDLTQALKINKPVIIEL
                     SVEGGIKIARQYPQVLLIFISTSEEFRTTVTRNQQSRKADLKHSAGEKRKAFEHYDYI
                     IENVEHYPNQAIDAIKDIITTHFPELKKKGII"
     gene            1120..1791
                     /locus_tag="VGE59_00475"
     CDS             1120..1791
                     /locus_tag="VGE59_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEY1074165.1"
                     /translation="MKLKQIPMQDALKKGWELTKENFLFFVGLLIINFIFSYSPYKES
                     GSTSSLFSGRGEYQPIVEVLLTIIRTVVEMGTIRIVLKLIDNKKPSYAEFFNQLNRFL
                     PFFLASILYIVLVGAGLILFIIPGIIFALTYQYYSYLILDKNMGIFEAFSKSAEITRG
                     VRWQLFLFGLLLILVMIAGFLALGVGLFVAMPVTMVAQAYVYRYLVKHSHKEIKEESD
                     EAIVV"
     gene            1920..>2356
                     /locus_tag="VGE59_00480"
     CDS             1920..>2356
                     /locus_tag="VGE59_00480"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074166.1"
                     /translation="MKRPWFTVVWLVIITMIAVAIVKPHGELNFYKFRKQFSFSEGLD
                     LQGGVHLKYILDLSEVAPQDREQATQGVKNVIENRINPLGIAEPVVQTAKVGNDAALI
                     IELPGQKDPEAAKDLIGKTAKLTFRESDEAGENFIETNLTGAD"
BASE COUNT          685 a          525 c          502 g          644 t
ORIGIN      
        1 ctccaggctc agtttcaaaa tcggactacc aaaaaagttt ataacgccct tgtattcggt
       61 gaaccgaaag aacattccgg cactattgaa acctttattg ggcgccatcc caaacggcga
      121 caagaacaag ccgtcctacc aatccaggta ggggagcgag cgcgaagaga agctattacc
      181 gactacattg taaaggcagt ttacttttat catgggcaac caattagttt aattgagttt
      241 tccccgaaga cagggaggat gcatcagctt cgagttcatg caaagtacat cggtacgcct
      301 atcctcggcg accaaacgta cacaattaag ccagccaaac gtatgagtaa agaagttggg
      361 cttactcggc agttactgca tgcatatcgg ctgacgattg agcatcctga aacacactct
      421 ccgatttctt tggaggcccc cttgcctgac gacatggtta accttctcaa actgattgat
      481 tcgtaatcga ttgatttcag actttttatt gtacttatta accgagtcat cgtatacttc
      541 ctatatgggg cacacatatc cgccaattat tattgtttct ggcgctgtcg gtagcggaaa
      601 ggggacggta attcatgcgc tcgcgcatga attagggctt gagtgggttc ctacgcatac
      661 cacccgagct catcgcccgg atgacgaaac attaagcaga cacgtcttcg acactgaacc
      721 aacctttctg cgtcaccttg cacgcaatga ataccttgag acaattgagt ataaaggaga
      781 acggtacggt ttacttcgcg aagaccttac gcaagctctc aaaataaata agccggtgat
      841 tattgaattg agtgttgagg gtggaataaa aattgcccgg cagtatcctc aggttcttct
      901 tattttcata tcgacatctg aggaatttcg cactactgtt acccgcaacc agcagagtcg
      961 aaaagctgat cttaaacact cggcaggaga gaaacgcaaa gcgttcgaac attacgacta
     1021 tattattgag aacgtcgaac actatcccaa ccaggccatt gacgccatca aggacattat
     1081 tactacccat tttcctgaat taaaaaagaa aggaatcata tgaaattaaa acaaatcccc
     1141 atgcaagatg ctttgaaaaa agggtgggaa ctcacaaaag agaattttct ttttttcgtt
     1201 ggcctcttaa ttattaactt tattttttcg tactctccct ataaagaaag tggtagtact
     1261 agctcactct tcagtggaag aggggagtat cagccaatcg tggaagtgtt actcacaatt
     1321 attcgcaccg tcgtagagat gggaactatt cgcatcgttt taaaactgat tgataacaaa
     1381 aagccaagtt atgctgagtt ttttaaccag ctaaaccgtt ttcttccgtt tttcctggca
     1441 agcatattat atattgtttt agtaggggca ggacttatcc tgttcattat tcccggtatt
     1501 atttttgcct taacatatca gtactacagc tacctcattc tcgataaaaa catgggcata
     1561 tttgaagcct tttctaaaag tgcagaaata actcggggag tccgctggca actcttcctg
     1621 tttgggttat tgcttattct cgtcatgata gctggtttcc tcgcgttagg ggtcggtctg
     1681 tttgttgcca tgccagtaac catggtcgcg caagcttacg tatatcgcta cctggttaaa
     1741 cactcacata aagagatcaa ggaagaaagc gacgaagcca ttgtggtgta attaacagct
     1801 tcattgcgtt tccacgaact cacttggttc accgcttcgt gcgagaagta agtgagttct
     1861 ttgcattttc tcgagagttg ggctagaatg agccaagtta caaaaggaag aagcttataa
     1921 tgaaacgacc ttggtttact gtcgtgtggt tagttattat tacgatgatt gccgtcgcta
     1981 ttgtgaaacc ccacggcgaa cttaattttt ataaattccg taaacagttt tcttttagtg
     2041 aaggattgga ccttcaaggt ggtgtccacc ttaaatacat cctcgatctc tcggaagtgg
     2101 caccccaaga tagagaacaa gcaacccagg gtgtcaaaaa tgtgattgaa aaccgaatca
     2161 acccattagg catcgccgaa ccggtcgttc aaaccgctaa ggtgggaaac gacgcagccc
     2221 ttattattga gctacctggc caaaaagatc cggaagccgc caaagactta attggaaaaa
     2281 ccgcgaaact cacctttcga gaaagcgatg aagccggcga aaactttatc gaaacgaatc
     2341 tcaccggcgc tgattt
//
LOCUS       DASWYB010000009        10408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_568489, whole genome shotgun sequence.
ACCESSION   DASWYB010000009 DASWYB010000000
VERSION     DASWYB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568489"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VGE59_00485"
     CDS             <1..804
                     /locus_tag="VGE59_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY1074167.1"
                     /translation="KYVRSRETTSDFDRARRQQEVLKGIKDKAISAQVLTNPKKITDL
                     ITVLGRHILSDFTVSEGEQLLAIAHDFDNPTIRSQVLDTSEGGLLTSTRNDAGSYILV
                     PKAGLNDYSEIQAFIKAYFNAPAINAEKPTITIQNGGITKDQFTRTAKYLEGAGFAVT
                     KNTTSSEAVAKTTLHDFSSKKPISQKFLKDWLDLAPRSEVAPTPTTDFVLILGTDFKK
                     VVQAAEKQTVKSENSTTSPLKVSPSPMPDDSLTFGDGGYSRTSSTTLIP"
     gene            814..1410
                     /locus_tag="VGE59_00490"
     CDS             814..1410
                     /locus_tag="VGE59_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074168.1"
                     /translation="MEKHFVTPKFPLGLTLGLLLVVLVELAVYGVVIAIPALWFALVF
                     GGFYLSYVALLRHDVLPLVFVSLFLTAHHTLFFNYHSDMPMALLFLIIFGVNSIIMWL
                     LLHYATHLKPEYHMAYSLMSGFMIAQILTLFASMAKDWSFRFELASYVSVVFSYSFWR
                     FACLAADSLLTSKQFLRVAVVVIILIVAIILGSPNVQV"
     gene            1425..2405
                     /locus_tag="VGE59_00495"
     CDS             1425..2405
                     /locus_tag="VGE59_00495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1074169.1"
                     /translation="MKSFFVPIFQKIKNSLTSIRIAWIKYWALQRRFAFDNEPMSFRK
                     AFVLFISGILAIYFLIGLPVLGYMIYVRNSEGVPLAIASALYPFPVATVGGDVILLKP
                     YSDRLAYLKFFSKQTQQALPEGDALRKQVIDKLIDEAVIRQWAEKEGISVTRDDINAA
                     YDKIIQDRGSESDVKTVLSQLYNLNDAEFKRLIPDLLYREKIERSLLERVRIKHILVS
                     TETLAKKVKSEVTPENFDEQAKKYSEDKATRDAGGDLGYFDRARAEKLAPELAQVFWD
                     LPTGQVSDPIKTAFGYHILVVTERSGKERKTFAQWLDEKRGQTKIRRLLK"
     gene            complement(2512..2874)
                     /locus_tag="VGE59_00500"
     CDS             complement(2512..2874)
                     /locus_tag="VGE59_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY1074170.1"
                     /translation="MRLSRKSLYAIIGIIVLLFVVAIYRGYQINADVATKNVAISEVT
                     PTSGPTGTEILIRGEGMLTNENTVTIGDKTVTGLTSDDGTSLLFYVPGDLTAGSYTLA
                     VKNSLGQSNTSTFTVTAQ"
     gene            complement(3032..3496)
                     /gene="bcp"
                     /locus_tag="VGE59_00505"
     CDS             complement(3032..3496)
                     /gene="bcp"
                     /locus_tag="VGE59_00505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY1074171.1"
                     /translation="MLTLGSAAPIFTLPNQDGKVVSLSDFLGKWVVVYFYPKDDTPGC
                     TVEACSFRDAESELTDAGITVLGISKDSVASHKKFADKFNLKFHLLADPSTEVIQAYD
                     AWQEKSMYGKKFMGIQRSTVLINPDGKITKIFPKVTPKDHAKEILKAVQESE"
     gene            complement(3593..4105)
                     /locus_tag="VGE59_00510"
     CDS             complement(3593..4105)
                     /locus_tag="VGE59_00510"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEY1074172.1"
                     /translation="MHEMIVAAFPDRSKADKAIEHLENKGYSEKDISVITRQSDTKDV
                     RTVGESDQAADVGSAAVTGGTLGGVAGLLLAAAGIIIVGPIAAALGLAGVAGTVASGA
                     VIGAVAGGLTAALTNLGIPAEQAKEYDRVVQEGGVILAIPVKDLDKDEIRRILEGNGA
                     EGTSEIEMKH"
     gene            4354..4674
                     /locus_tag="VGE59_00515"
     CDS             4354..4674
                     /locus_tag="VGE59_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY1074173.1"
                     /translation="MANQNGNRNKRGSQREDQEREGMSREEAGHLGGEATKEKYGPDF
                     YEEIGRKGGEATAETHDSDFFQEIGHEGGEATKRKHGPDFYREIGSQGGQARWEDQRE
                     ESDQ"
     gene            complement(4778..5128)
                     /locus_tag="VGE59_00520"
     CDS             complement(4778..5128)
                     /locus_tag="VGE59_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEY1074174.1"
                     /translation="MAARYHIIIDVSEVSEPALVSQSGINRLLTDLPAKIDMKILHGP
                     VVIEGIPENPGVTGFVVIDFSHISVHTFTQTSQALVDIFSCKKYSQNEAEDFVLRYFG
                     VTKGQAKIQVVSWG"
     gene            complement(5167..5709)
                     /locus_tag="VGE59_00525"
     CDS             complement(5167..5709)
                     /locus_tag="VGE59_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1074175.1"
                     /translation="MTQVVFTHRALHKKEASLVSKKLRETPYITGYMPDELVCLDNSN
                     IAEVDGRFAGVCITKDISSRWSEIAMMYVLPEFRNQGIGTTLFDQAFQSIRYKKRHLY
                     CVSRNKSVIKLLHSQGFVFRSRIWQLPLPVILHIIRFSLNFYRVKEFVRKLFAFQKQD
                     PFVYALLPYSKPVVRETADR"
     gene            complement(5709..6641)
                     /locus_tag="VGE59_00530"
     CDS             complement(5709..6641)
                     /locus_tag="VGE59_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY1074176.1"
                     /translation="MTPIIITDNENPDLDGTACIYAYTEFLKTQGESPLGAIYGTPQR
                     EALFVLDCFKVSPLPIADQLVTPQSQVILVDTSVLRTIAAEQINPTQVIEIIDHRQFN
                     HAESFPSAKVQIERVGAAATLIAEKFQAVKIKPTYESAGLLYSAIISNTVNFKNNVTT
                     DRDRAMATWLKAGLNLPEDYIDQMFTAKSKLNGTLEEVIRSDVALNELGGKRFGIAQQ
                     EIVQAKNFIEDNKDELHRILQTIKKEKLLDHIFLSCIDVLEGKNYFFFIDPETEALTT
                     RIYNQHGHPIMMRKEIFPAIKQAVEKDPAKQNQA"
     gene            complement(6643..7743)
                     /locus_tag="VGE59_00535"
     CDS             complement(6643..7743)
                     /locus_tag="VGE59_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY1074177.1"
                     /translation="MSKAVINEHQPSMTEWFAAIGEEKESQAFREEDLKKQAKLETLY
                     QTINLTYERPEIWPATEITEENPAFAELLKTRGKELCAFRLVPQKPGLEKIRNRGLSI
                     QDCYETWFKNLTINREDYKVEICPHSDTLLWSTIFVVNKDMVFGEIIQGMHNQLTQGN
                     TISTPYRFQYNYKDWVWSEDNAEAKHQMERTLAMLKVEDIQKQAELKQALNANFSHGY
                     LVGYFETTVWEGDKIHFIDYNRLLPQFFLTPPPMNNEKQSDTTILTGTPAFAGSATGT
                     VVIVPEDKINSVDFPEGSILVCDNTDVRYLPLMKKSAAIVTNRGGMLSHAAIVARELK
                     IPCLVGTKTATKTLSTGMHIQVDASAGTICKA"
     gene            complement(7855..8523)
                     /locus_tag="VGE59_00540"
     CDS             complement(7855..8523)
                     /locus_tag="VGE59_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1074178.1"
                     /translation="MSRSREIVRTATVCTYLFRHGETPTLPDGKREDRLTPRGERQAA
                     LAGMTHLKNLQVLGILSSGTRRSTETIRTACTAAGMEVPGVEVVAAIGFSWIDDQVPP
                     CDFYAIERRLHEEYGGSRRVPIYAWLEAYPHAQATRGRLTEFLHHRAKQEFMEWPLHS
                     MINLVGGSHGPTAATAALNTRETMGLDFADGIRYDVEVVRYDTDEIETKLVRSRVLTC
                     PDCK"
     gene            complement(8671..8865)
                     /locus_tag="VGE59_00545"
     CDS             complement(8671..8865)
                     /locus_tag="VGE59_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY1074179.1"
                     /translation="MQLGDILVAPMTTPEYSPVFSKVKAIVTDEGGVTCHAAVVAREH
                     QIPTIVGTKKPAKLLQIAKK"
     gene            complement(8910..9722)
                     /locus_tag="VGE59_00550"
     CDS             complement(8910..9722)
                     /locus_tag="VGE59_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074180.1"
                     /translation="MNTLEFEPSAFRNWTVAYCQLAYHMWVTEFRRQFGWSLTDMCYE
                     WNGSYTTMYRAPSEHIEKLFHFVAEESQNDPDYFERIAHEAEQESADEATYFSSLQEL
                     DFTAVDSEKLKMLFQDFCAYVLPPMPKFFIVMYFPQALELHPEAQDKFKADNDRLIEA
                     RGKIDKIAAPIGNMLTEKFGHAALARYGLDISLARYLTLSEIESLFANTIDKEQLVDE
                     LSKRQRYCLIAGGEVVEMTIQDYLTSKGWRLVAESEPQQLQGSVAHSGSEKK"
     gene            complement(9719..>10408)
                     /locus_tag="VGE59_00555"
     CDS             complement(9719..>10408)
                     /locus_tag="VGE59_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY1074181.1"
                     /translation="ANLRDLAAFKDVMRAFINKAFFEPASYLSSLFDKLSTQFSVSTK
                     NLNRYLQKDILALYDGAYVADDELSARTHAFVTFTYEDRPFTLSGTDCLEIIRDFEDI
                     IIPDQNLLKGQTANGKSIIQGKVKVIPVDYSDFNRVNAEIEKMEPGDILVAETTAPEL
                     MLACKKAAAIVTDLGGLLSHAAIVSRELGIPCIVNTKHASRVLKDGDIVEIDTTRGVV
                     RRIELIKNTLK"
BASE COUNT         2682 a         2276 c         2433 g         3017 t
ORIGIN      
        1 aaatatgttc gttcccgaga aactacttcc gactttgacc gcgcccgtcg tcagcaagaa
       61 gtgctaaaag gaattaaaga caaagccatt agtgcccaag ttctcaccaa ccccaaaaag
      121 ataactgatt taatcaccgt gcttggtcgc catatccttt cagactttac ggtgagcgaa
      181 ggtgaacagt tgcttgctat cgcccatgat tttgataatc ctactatccg gtcgcaggtt
      241 ctcgacacga gtgaaggtgg cctactcact tcgactcgta atgatgcggg tagttatatt
      301 ttagttccca aggcagggtt aaatgattac tcagaaatcc aggcattcat taaagcctat
      361 ttcaacgctc cagctattaa tgcagaaaaa ccaacaatta cgattcaaaa tggtggtatt
      421 accaaagatc aatttactcg tactgcaaaa tatcttgaag gcgccggctt tgctgtcacc
      481 aaaaatacaa ccagctcaga agctgtcgcc aagacaacct tgcatgactt ctcatccaaa
      541 aaacctatta gccaaaagtt tcttaaagac tggcttgatc tggctccccg ttcagaagtc
      601 gctcctaccc ctacgactga ttttgtgtta atccttggga ctgattttaa aaaggttgtt
      661 caagccgcag aaaaacaaac cgtgaagtcg gaaaattcaa cgacgtctcc gctcaaagtc
      721 tccccttccc caatgcctga tgatagtctc acgtttggag atgggggcta ttcaaggacg
      781 agttccacaa cgcttattcc ctaggatata ggtatggaga aacactttgt cacgccgaag
      841 ttcccacttg gactgactct cggcctgctc cttgtcgttt tagttgaatt agccgtttat
      901 ggtgttgtca ttgctattcc tgcgctgtgg tttgctctcg tatttggtgg attctatctt
      961 agttacgtgg cacttttacg tcatgacgtg ttgcctctcg tttttgtaag tttattctta
     1021 actgcgcatc atacgctctt ttttaactac catagtgata tgcctatggc gctccttttc
     1081 ttaattattt ttggtgtgaa cagtattatt atgtggctac ttttacatta tgcgactcat
     1141 ttaaagcccg agtaccatat ggcctactcg ttaatgagtg gctttatgat cgcccaaatc
     1201 ctgacgcttt ttgcgtcaat ggcgaaagat tggtcgttcc gttttgaact cgcctcatat
     1261 gtctcggttg tttttagtta cagcttctgg cgatttgcct gtttggcggc cgattctctt
     1321 ttgacaagta agcaatttct tcgcgtggcg gtggttgtta ttatcctgat tgttgctatt
     1381 attctcggct ccccaaacgt tcaagtttaa gttatagtaa ggatatgaaa tccttctttg
     1441 ttccgatctt tcagaaaata aaaaatagcc tcacttctat tcgaattgct tggattaagt
     1501 actgggcgct acaacgccga tttgccttcg ataacgaacc gatgagcttt cgtaaagctt
     1561 ttgttctgtt tatctcggga attctcgcaa tttatttttt aattggtctg ccggttctgg
     1621 ggtatatgat ttacgttcgt aactccgaag gcgtgcccct tgcgattgcg tctgctcttt
     1681 atccttttcc tgttgctact gtcggaggag acgttattct cttaaagccc tactctgacc
     1741 gtctggcgta tttaaaattc ttttcaaagc aaacacagca agcgcttcct gaaggtgatg
     1801 ctctccgcaa gcaagttatc gataaactta tagatgaagc agtgattcga cagtgggcag
     1861 aaaaagaagg tattagcgtg actcgtgacg atattaacgc tgcttacgat aaaattattc
     1921 aagatcgggg aagtgaaagt gacgtgaaga cggtactctc acaactctat aacttaaacg
     1981 atgcagaatt taagcggttg attcccgatc ttctttatcg agaaaaaatc gaacggagcc
     2041 ttttggaacg tgtcaggatt aaacatatcc ttgtttcgac cgaaacactc gcgaagaaag
     2101 tgaaaagcga agttactcct gagaattttg acgaacaggc aaaaaagtat agtgaagaca
     2161 aagcaacgcg agacgccggt ggggacctgg gttattttga ccgggctcgg gccgaaaaat
     2221 tagcgccgga gttagcacag gttttctggg acctcccgac cggacaggtt tcagacccga
     2281 ttaaaactgc ctttggttac catattctgg tggttactga gcgttctgga aaagagcgaa
     2341 agacatttgc tcaatggctc gacgagaagc ggggtcagac aaaaattcgc cgattgctaa
     2401 agtagctttg agttgaattt aggcatcgcc ctatacaatc gtgtttcgcc aagtcgagag
     2461 aaaaacaaaa agcccggatt gctccgggct ttttgtttta agcgcttatt tttattgagc
     2521 ggtgaccgta aaggttgaag tgttgctctg acccaggctg ttttttacag ctaaggtgta
     2581 actgccggca gtaaggtcgc caggcacata gaacagcagc gaggtgccgt catcagaggt
     2641 gaggccggta acggttttgt ctccgatggt aactgtgttc tcgtttgtta acatgccttc
     2701 accgcgaatg aggatttctg tgccagttgg gccagaagtc ggtgtgactt cgctgatagc
     2761 aacgttttta gttgcgacat cagcattaat ctggtagcca cggtaaatag caaccacaaa
     2821 aagtaaaaca atgataccga taattgcgta aagtgacttt cgagaaagac gcatccgcgc
     2881 tcctttcgtt cttttataat cgtacctgtc tggacaccca gtgtcaaacc ggctacccaa
     2941 cctccctcct atctattaga gagtgaaaca agtagcaata ttatattcta tttttaaaaa
     3001 aatggcaata gtttgcctat aaaagttgtg gttattcgct ctcttgtact gcttttaaaa
     3061 tctctttggc gtgatctttg ggggttactt tggggaaaat tttggtaatc tttccgtcag
     3121 gattaataag tacggtactg cgttgaatgc ccataaactt tttgccatac atgctttttt
     3181 cttgccacgc gtcataggct tgaattacct cggttgatgg gtcggcaagt aaatgaaatt
     3241 ttagattaaa cttatcggca aactttttgt gtgaggcgac agaatctttg ctgataccaa
     3301 ggactgtaat cccggcatcg gtaagctcac tttctgcatc gcgaaaactg catgcctcga
     3361 cggtacagcc gggcgtgtca tctttggggt aaaagtaaac gacgacccat ttgccaagaa
     3421 aatcggataa cgaaacaact tttccatctt ggtttggaag tgtaaaaatg ggagcagcag
     3481 atccaagtgt cagcatgaag tatctcctga ggtaaggttt atgtgactcc actataccac
     3541 aggcgaagag aacattttaa aaggagccta ggttacaagg ctccttttgt aactagtgtt
     3601 tcatttcgat ttcactagtt ccttcggctc cgttgccttc gaggattctg cggatttcgt
     3661 ctttgtcgag atctttgacg ggaatcgcca gaatgacacc accttcttgg acgacccggt
     3721 cgtattcctt agcctgttcg gcggggatac cgaggttggt aagcgcagcg gttaatcctc
     3781 ctgctaccgc accaataacc gcaccagatg ccactgtccc ggccactcca gcgagaccga
     3841 gagctgccgc aataggtccg acaataataa ttcccgctgc ggcaagaaga agcccggcta
     3901 ctccccctaa cgtcccacct gtgacggcgg cagatccaac atcggcagcc tggtctgatt
     3961 ccccaaccgt tcttacgtcc ttcgtgtcac tctggcgggt aataaccgaa atatcttttt
     4021 cgctataccc tttattttcc agatgctcaa ttgctttatc ggcttttgaa cggtcgggaa
     4081 atgccgcaac aatcatttca tgcatagaac tcctttcgtt aatgcgttgt gatctgtaac
     4141 ataacttctt tctaaaataa gggcattagg agttttccta aggatggttc gtacaaaatg
     4201 aggttattca gtcagacgct acaacagttg tttgtagaac agcgcacaaa gtcttttttt
     4261 tgaagcggaa ggtgtcagct gtcttgccaa tgtattcgcc tccggtatgg cttaccatgg
     4321 gagtgcaagg aagtagagag gaggtgagag agcatggcaa accagaatgg aaatcgtaat
     4381 aagcgaggca gtcaacgtga ggatcaggag cgagaaggga tgtctcggga agaagctggt
     4441 cacttagggg gagaagccac aaaagaaaaa tacggcccag acttttatga ggaaataggt
     4501 cgtaaaggtg gcgaagccac agcggaaact cacgatagtg atttcttcca ggaaattggc
     4561 cacgaaggtg gtgaagccac aaagcgtaag cacggcccag acttttaccg agaaattgga
     4621 agtcaaggtg gtcaggcacg gtgggaagat caacgggaag aaagtgatca ataaagggcg
     4681 acgtagctcc gtagcacaaa acttatacaa aatataaaac gcgatttaaa agagggcttc
     4741 caccaggagc cctcttcgcg atccgggatt cgggttttta tccccaggaa acaacttgaa
     4801 ttttagcctg gccttttgtg actccaaaat acctcaggac aaaatcttct gcttcgtttt
     4861 ggctatattt cttgcaagaa aaaatatcaa cgagagcttg gcttgtttgt gtaaaggtgt
     4921 ggactgaaat atgggaaaaa tcaatgacaa caaagcctgt taccccggga ttttcgggga
     4981 taccttctat taccacaggg ccatggagaa ttttcatatc aatcttagca ggcaggtcgg
     5041 tgaggagccg gttaatcccg ctctgactga caagtgccgg ttcactaact tcggatacgt
     5101 cgataataat atggtaacga gctgccacgc ggtcccttct ttttattcat aaaatcactt
     5161 tctatcttac ctatctgctg tctccctgac aacgggtttt gaatatggga gtaaggcata
     5221 cacaaaggga tcctgttttt gaaaggcaaa aagttttcgt acaaactctt ttacacggta
     5281 aaagtttaaa gaaaatcgga tgatgtggag tattacaggt aaggggagtt gccatatccg
     5341 cgaacggaat acgaaaccct gactatggag cagcttgatc acggatttat tccggctgac
     5401 gcagtacagg tgcctctttt tgtagcgaat agattgaaaa gcttgatcaa aaagagttgt
     5461 tccaatgccc tgattgcgaa attcaggaag gacgtacatc atcgcaattt cagaccaacg
     5521 agaagaaata tctttggtaa tgcaaactcc ggcgaaccga ccatcaactt ctgcgatgtt
     5581 actgttatcc aagcaaacca attcatccgg catgtaaccc gttatgtatg gagtctctct
     5641 taattttttt gatacaagcg aggcttcttt cttatgtagg gctcggtgcg taaatacgac
     5701 ctgtgtcatt atgcttggtt ctgtttggca ggatcttttt cgacggcttg tttaatcgca
     5761 gggaaaattt ctttgcgcat cataatagga tgaccatgtt gattatagat gcgtgttgta
     5821 agagcttcgg tttcggggtc aataaagaag aaatagtttt ttccttccag gacatcaata
     5881 cagctgagaa atatatgatc gagcaacttt tcctttttga tagtctgaag aatacgatgg
     5941 agctcgtctt tgttgtcttc gataaaattt ttagcttgca caatttcttg ttgggcaatg
     6001 ccaaatcgtt tgccacccaa ctcgttgagg gcaacatcgc tccgaatcac ttcttcaaga
     6061 gtgccgttga gttttgattt agcggtaaac atttggtcaa tataatcttc aggaaggtta
     6121 agcccggctt tgagccatgt cgccatggct cggtctcggt cggtggtgac gttattttta
     6181 aaattgacgg tattggaaat aattgccgag tacaataatc cggcggattc atatgtgggt
     6241 tttattttta ctgcttgaaa cttctcggcg ataagagttg cggcagcgcc aacacgttca
     6301 atctgaactt tcgcagaggg gaatgattcg gcgtgattga attgtcggtg gtcgataatt
     6361 tctatcactt gagttgggtt aatctgttcg gctgcaattg tgcgaagcac gctcgtatcg
     6421 acaaggatta cttggctttg aggggtaacg agttgatcgg cgatagggag aggggaaacc
     6481 ttaaaacaat cgaggacaaa aagagcttcc cgttggggag tcccataaat tgctccgaga
     6541 ggtgattccc cctgggtttt taggaattca gtataggcat aaatgcaggc cgttccgtcg
     6601 aggtcggggt tttcgttatc tgtaatgata ataggtgtca tactaggcct tacaaatagt
     6661 accggcggag gcatccactt gaatgtgcat accggttgag agtgtttttg tagcggtctt
     6721 cgtgccaact aaacaaggga ttttaagttc gcgagcgacg atggctgcat gagaaagcat
     6781 gccgccgcga ttggttacaa tggcggcaga ctttttcata agaggcaggt agcgaacgtc
     6841 tgtgttgtcg cagacaagaa tagagccttc gggaaagtca acggagttta ttttgtcttc
     6901 aggcacaatt accaccgtac cagttgcgct cccggcaaag gcgggtgtgc cggttaagat
     6961 tgtcgtgtcg ctttgttttt cgttattcat cggcggcgga gttaagaaaa actgaggtaa
     7021 gaggcggttg tagtcaatga agtgaatttt atcaccttcc caaaccgtag tttcaaaata
     7081 gcctacaaga tagccatgcg aaaagtttgc gttcagagct tgttttaatt ctgcttgttt
     7141 ttggatgtcc tcaaccttaa gcattgccaa ggttcgttcc atttgatgtt tggcttctgc
     7201 gttgtcttca gaccacaccc agtctttata gttatactga aatcggtacg gcgtcgaaat
     7261 ggtgtttcct tgggtaagct gattatgcat accttgaatt atctccccaa aaaccatatc
     7321 cttattgacc acaaagattg tagaccaaag aagcgtgtct gagtgaggac agatttcgac
     7381 tttgtaatct tctcggttaa tggtgaggtt tttaaaccag gtttcgtaac agtcttgaat
     7441 cgagaggcca cggtttcgga ttttttcgag accgggcttt tggggaacca agcgaaacgc
     7501 acagagctcc ttaccgcgtg ttttaaggag ttccgcaaag gccgggttct cttctgttat
     7561 ctccgtggcg ggccaaatct cgggtcgttc atacgtaagg ttgatcgttt ggtaaagggt
     7621 ctcaagtttt gcttgctttt ttaaatcttc ttcgcggaat gcctgcgact ctttttcttc
     7681 gccgatcgcc gcaaaccatt cggtcataga aggttggtgt tcgttaataa cagctttact
     7741 catatctgtt ttgtctcata ttttaagaga aaaggtcaat aagaaaaggg ttccggacgt
     7801 acgcacagct gtgcgtagcc ggaaccctat gatccttgcc tgtcgcaaga atggttactt
     7861 gcagtcgggg caagtgagca cacgtgagcg cacgagtttt gtctcgatct cgtcggtgtc
     7921 ataccgcacg acttccacgt cgtagcgaat gccatcggcg aagtccaagc ccattgtttc
     7981 ccgggtgttg agcgccgcag tggctgccgt gggtccatgc gagcctccca ccaggtttat
     8041 catgctgtgg agtggccact ccatgaattc ctgtttagcg cggtgatgca ggaactccgt
     8101 aagacgtccc cgggtagcct gggcgtgcgg ataggcctcg agccaggcat agattggcac
     8161 tctccgtgat ccgccgtact cttcatgtaa acggcgctcg atggcataga agtcgcatgg
     8221 tgggacttga tcgtcaatcc acgaaaatcc gattgccgct acaacctcaa caccagggac
     8281 ttccattccc gccgccgtgc aagcggtccg gattgtctcg gttgagcggc gtgtaccgct
     8341 cgatagtata ccgagcacct ggagattttt caggtgcgtc atgcccgcca gagcggcttg
     8401 cctctctcct cgtggtgtga ggcggtcttc acgcttaccg tctgggagtg tcggtgtctc
     8461 cccgtgccgg aagaggtagg tacaaacggt cgcggtccgc actatctctc gagatctgct
     8521 cattacgttc cccctagttt ccccaatacg tgagcggttc caaagaacag gtaaaatata
     8581 ccatatttta cctgtttccg tcaatccata gtgatcgtaa atattacttg tttgttaaaa
     8641 tactgactgt gccagatgaa agatccagct ctatttcttg gctatttgta ataacttggc
     8701 tggctttttt gtgccaacaa tagtagggat ttggtgctcg cgtgctacta ctgcggcatg
     8761 acatgtcacg cccccttcgt ctgtcacaat ggctttcacc ttcgaaaaca cgggactata
     8821 ttcgggtgtg gtcatagggg caaccaaaat gtcacctagc tgcacttgat gaagtgatga
     8881 tgtatttaga ataattcgtg cagtcccttt tatttttttt cagaaccaga atgcgcaaca
     8941 ctcccctgga gctgttgggg ttcggattcg gctaccaatc gccatccttt gctcgttagg
     9001 tagtcttgga tagtcatttc cacaacttct ccaccagcga ttaaacagta tctttggcgt
     9061 ttgctcagtt cgtcgacaag ttgttctttg tcgatggtat tcgcaaaaag gctttctatt
     9121 tcacttaacg taagatagcg agccaatgaa atatctaaac cataacgtgc taatgcagca
     9181 tgaccgaatt tttctgtcag catattacca attggcgctg caattttatc gattttgccg
     9241 cgcgcttcaa tgagacgatc attgtcagct ttaaacttgt cttgtgcttc agggtgtagc
     9301 tctaatgctt gcggaaagta cattacaata aaaaatttgg gcattggcgg taaaacataa
     9361 gcacaaaaat cctgaaagag catttttaac ttttctgaat caaccgccgt gaagtctaac
     9421 tcttgtaacg agctaaagta agtagcttca tcggcagatt cttgttctgc ttcatgtgct
     9481 attctctcaa aataatcggg gtcattttgt gactcctccg ccacgaaatg aaagagcttt
     9541 tctatgtgct ctgaaggagc acgatacatc gtggtatagc ttccattcca ctcataacac
     9601 atgtctgtga ggctccatcc aaattgccga cgaaattcag tgacccacat atgataagcg
     9661 agctggcaat aagcaacagt ccagtttcga aaagccgaag gttcgaattc taaggtgttc
     9721 attttaaagt attctttatt agctctatac gtcttacgac gcccctagtt gtatctatct
     9781 caacgatatc tccatcttta agcactcgag atgcgtgctt tgtattgacg atacaaggga
     9841 ttcctaactc gcgcgataca attgcggcat gggaaaggag tccgcctaga tcagttacaa
     9901 tcgcagccgc ctttttgcag gcgagcataa gttcgggggc agtggtttcg gccaccaaaa
     9961 tatcgcctgg ttccattttt tctatttctg cattgacccg attgaagtcg ctgtagtcta
    10021 cgggaataac ttttactttc ccctgaatta tggactttcc attcgcggtt tgtcctttaa
    10081 gaaggttttg atcaggaata atgatgtctt caaagtcccg aatgatctcc aaacaatctg
    10141 ttcctgatag ggtaaaagga cggtcttcgt atgtgaacgt tacgaatgca tgtgttcgtg
    10201 cgcttagctc gtcatcagca acatacgcgc cgtcatagag ggctaaaata tctttttgaa
    10261 gatagcgatt aagattcttt gtactcacag aaaactgagt actgagttta tcgaataagc
    10321 tcgataaata actggcaggt tcaaaaaaag ctttgttgat aaaagcacgc ataacatctt
    10381 taaatgcagc taaatctctt aaattggc
//
LOCUS       DASWYB010000010        11113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_214752, whole genome shotgun sequence.
ACCESSION   DASWYB010000010 DASWYB010000000
VERSION     DASWYB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214752"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1876
                     /locus_tag="VGE59_00560"
     CDS             116..1876
                     /locus_tag="VGE59_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074182.1"
                     /translation="MKYTLTNSQAPTPNESMFAVLKKLWPYITNEKLRLVGAFLAILC
                     NSGLTILAPILVGRAVDHYIIRGDYHGVLVYVGYLLATYLGMLLSGYIQTRIMGIVGQ
                     RILFNLRNAIFTRLQELPIAFFNQNKGGDLISRINNDTDKLNQFFSRSLMQFVGNVFV
                     IIGAGIAMLVVNFTLGSAALVPAVVLLVFAQLTTEWVKRKNLENLQTTGGMSAEIAES
                     LQNFKVIIAFNRRDYFRKKFEEVNEKNYHASISSGIANSTYVPTFGLASNVGQMIVIV
                     YGLYLISQGSFTFGLLISYLAYVSRFYDPLRQMAALWSDLQTALAAWERIGEILAMKS
                     NLTVVKASEDAKGKGIIEFKKVGFQYPDGAPVLTNINFVMYPGKTYALVGPTGGGKTT
                     TASLIARLYDPTEGTVYLNGQDIRTYEADVRTAKIGFILQDPFLFTGTVRENILYGNN
                     AYAEYTNEQLVELLEREGLSDLLKRFDQGLDTPVTTSADGISLGQKQLIAFMRALLRH
                     PDILILDEASANIDTVTEQILDEILKRLPAQTARVIIAHRLNTIENADEILFVNGGKI
                     TPAGSLDHAVELLTSGVKSS"
     gene            complement(1953..2447)
                     /locus_tag="VGE59_00565"
     CDS             complement(1953..2447)
                     /locus_tag="VGE59_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074183.1"
                     /translation="MKTKYLFPILGLAALGVVSLGAMHTLAHDTAASDGKTLVQRLSE
                     KFNLKESDVEQVFTEHKSQVQATMEKRIDDKLTQAVTNKKITEEQKQKIIAKQKELQT
                     QMEATKETLKNMTPQERQAEMKKQRDALEQWAKDNGIDTQYLRGLGFKGVHRGGMRGG
                     HRPF"
     gene            complement(2507..3205)
                     /locus_tag="VGE59_00570"
     CDS             complement(2507..3205)
                     /locus_tag="VGE59_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074184.1"
                     /translation="MLEVKDLTKEFHAADIQVKAVDTLSFQVPAGSFAAIVGKSGSGK
                     STLLALLGALERPSHGSIKVDDQDITKLGDSKLIRYRREKIGFIFQHYNLIPNLTALE
                     NVMLPMEFAGLPTKERRERAEHLLNEVGLTDDKQHRRPGRLSGGEQQRVSIARALANK
                     PKLILADEPTGNLDSNTSKMIVELLRHLAKTEQTTILVVTHDLNITKHTDMTFQLKDG
                     KLIHHAGHIKELVS"
     gene            complement(3207..4562)
                     /locus_tag="VGE59_00575"
     CDS             complement(3207..4562)
                     /locus_tag="VGE59_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1074185.1"
                     /translation="MSILVHGARNAFRNTIRTFSITLILALSIGLALIMFLAYRTVSA
                     RIETVKSTIGNTITVNPAGARGFEGGGEPLTVSQINDLSNLEHVNKVAATLSARMTAG
                     TDTSLVTAIEAGTLGLRQNRFNQRDVPTISPGTQGQTRTFTIPIMVTGIKDTSILTTG
                     STSVISGNVFNGDSDDNVALLGKSLAEKNGLMTGSTFTAFGTTITVSGIYDAGNQFAN
                     SSLYMPLKTTQRLSGQTDEVSVATVSVDSVTNLDATVTAIKNKLGEDKADVTSNEESA
                     KQALSPLENVKNISFYSLIGSLAAGAIITLLTMIMIVRERRKEIGVLKAIGASNIRVV
                     AQFITESLVLTVLGSIVGMILGFFLSNPILSALVANSSTTDGSTAGRMIAGPGGGGFM
                     RISQLGSTVQTNLRDIHSVMSYDILLYGLLAALIIAIIGSALPAWLIAKVRPAEVIRG
                     E"
     gene            complement(4661..5716)
                     /locus_tag="VGE59_00580"
     CDS             complement(4661..5716)
                     /locus_tag="VGE59_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1074186.1"
                     /translation="MNSFGTPLFRSASLRLTLWYLLILMVLSGLFSGVLYQVSTQEFN
                     REIRRFESMQQSTLFAPRGFTEFRRLRLEEIAQSKQHILMNLVYFNVVILVAGGGLSY
                     LLAQRTLEPIEESLEAQHRFITDASHELRTPLTAMKTELEVALRDKNLTFEESKQLHA
                     SNLEEVGKLETLANNLLKLAKPPTQAALHAFETCQTGDIIQAAITKIAPLAKSKEIVI
                     DNSVRNSEIEGDERSLTELFTILLDNAIKYSPSHTKVTISNTIKRKQKEIIFHIDDEG
                     IGIKASDFPHIFERFYRADASRSKQIADGHGLGLAIAKKITEMHHGSIDATPRTENGT
                     RFTVTLPISQPSSRLAE"
     gene            complement(5713..6393)
                     /locus_tag="VGE59_00585"
     CDS             complement(5713..6393)
                     /locus_tag="VGE59_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1074187.1"
                     /translation="MRLLVIEDEHKIANALKKGLELESFAVDVVYNGKQGMSYALDES
                     YDLIILDRMLPDITDGMEICRNVRDKGLHTPILMLTAKDQVRDRVGGLNAGADDYLVK
                     PFAFEELLARVRALLRRPQETTHSLLKTDDLRVDTVNFAVTRAGKPISLSTKEYALLE
                     YLMRNKGRTLTKDQIISHVWDYDADILPNTVEVYIGYLRSKVDKPFPSRPLIHTIRGF
                     GYRFGEKA"
     gene            6494..6937
                     /locus_tag="VGE59_00590"
     CDS             6494..6937
                     /locus_tag="VGE59_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY1074188.1"
                     /translation="MSNKSILLTPDTSLKLVISMARAQSIISRYFDGRLGGGIGLNDF
                     LILYYLTSAEGNKLRRVDLAHKMGFTPSGVTRLLAPMEKIGLIKREPSQHDARVSYVS
                     LAPSGQRILQEEFERAELLAQEVLPSSHEVGDFEKFLSVIGKVTS"
     gene            complement(6982..8433)
                     /locus_tag="VGE59_00595"
     CDS             complement(6982..8433)
                     /locus_tag="VGE59_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1074189.1"
                     /translation="MAQGDVIISFDRVSFGYDPLKPLLDEVEFSIRERAKFTIMGQNG
                     AGKSTLFKLIAGQLKPQSGRINIRDDAKIATSLQVVPRDKLNVTVQEFFAGAFKDVPR
                     DLERRITSVLETVNLVAPLTKKMNEFSGGQQARLLLAYGLIQEPDILLLDEPTNNLDP
                     TGIWHLTEFLINYPKTCIVISHDANFLNMFTDGVLHLDVFSRKVTKYLGDYYSAVDAI
                     EAQIERDRRQNARLEKEIQDNKDKVNFFANKGGKMRKLASKLRDSIEEAEENKAEVRR
                     EEKTLGHFTIPFQDFPDAIVTINSVTFLKDHTPVTKPVDIQLRKRQRLLLEGSNGIGK
                     STLLEALARGTQAGAKINPEVKVGYYRQDFSGLNFDATVRESLEEMDPAADNETIYRT
                     AARFLITSDLLKNKVGSLSEGQKGLLCFARFTLQQPGLLILDEPTNHINFRHIPVIAQ
                     AIQDYEGAVVLVSHVPEFVEQIKFTETLDLGKL"
     gene            complement(8499..8813)
                     /locus_tag="VGE59_00600"
     CDS             complement(8499..8813)
                     /locus_tag="VGE59_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY1074190.1"
                     /translation="MATHSTTIDFLLEQIQSAGNVRARKMFGEYALYCNDKVVALVCD
                     DQLFVKQSSISSHFSEFYHEAPPYPNAKMYLQVSSEKWDDADWLSNFIRQTAEALPAL
                     KK"
     gene            8868..9404
                     /locus_tag="VGE59_00605"
     CDS             8868..9404
                     /locus_tag="VGE59_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY1074191.1"
                     /translation="MVYIAFLRGINVGGHTVTMQRLRELFEELGLQNVRTYIQSGNVF
                     FSTSETDRVNLSQIIELHLRSALGYAVPTFLRTCEEVAYSLEVSPFKGIEQPEDKRFF
                     IMFLSTAVSPLTLPIASPKKDYEIVGQTETELFVILDLSVGRPGNPNVFIEKNFQVQT
                     TSRFYHTTRKILDAAQSI"
     gene            9629..10849
                     /locus_tag="VGE59_00610"
     CDS             9629..10849
                     /locus_tag="VGE59_00610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1074192.1"
                     /translation="MYNRSPRPFGSSSQRFSRRPNQRKFTGKYISIDRFIQKASMTTP
                     VEVYVPKHNFADFSLNKILKANILGKGYSTPTAIQDRTIPVSLEGRDVIGIANTGTGK
                     TAAFLIPLIHKVSNHKYDRVLIMTPTRELALQIQQEFKEFARGMNQYATLVIGGAPIG
                     AQIAQLRRNPQFVIGTPGRLKDLVERRVLNLSGFRSVVLDEADRMLDMGFIDDIQLLL
                     SGLSEERQTLFFSATMSPQIQALTRNFLKDPVMISVKSQDTSANVDQDVIHVNHEDDR
                     IEQLHELLVQPSYNKVLIFGQTKYGVESLSNELADRGFRVACIHGDKTQPKRQRALNM
                     FKQEDVQILVATDVAARGLDIPNVSHVINYDIPSTYEDYVHRIGRTGRANNKGYALTF
                     VTAHAQPSHNRYSR"
BASE COUNT         2898 a         2340 c         2536 g         3339 t
ORIGIN      
        1 caaattctcc ttctgatgga aggcgagctc ttggcgaaag gaacgcataa agagcttctc
       61 aagcaatcac cagaatatat gcagatctat acaacccaac agagtaccaa cacctatgaa
      121 gtacacactc actaattccc aagcacctac tcccaacgaa tccatgttcg cagttcttaa
      181 aaaactgtgg ccgtatatta ccaacgaaaa gttacggtta gtcggtgcgt ttctggctat
      241 tttgtgcaac tccggtctaa ctatcctggc acctatttta gtcggacggg cggttgacca
      301 ctatataatt cgtggagact atcacggtgt cttggtttac gttggatatc tcttggcaac
      361 gtatctcggt atgttgctga gtggatacat tcaaactcga attatgggca tagtgggaca
      421 gcgtattctt tttaacctcc gcaacgccat ctttacgcgg cttcaggagt tgccaattgc
      481 tttctttaac caaaataaag gtggcgattt aatctcccgc attaacaatg acacagataa
      541 actcaaccaa ttcttttctc gttccttgat gcagtttgtt ggtaatgtct ttgtcattat
      601 tggggctggg attgccatgc ttgtcgttaa ttttaccttg ggaagcgctg ccttagttcc
      661 tgctgtcgtg ctgctcgtgt ttgcgcagtt aacgacagaa tgggtaaagc gtaaaaacct
      721 agagaatctc cagactacag gtggtatgag tgccgagatt gctgaaagcc ttcaaaattt
      781 taaagtgatt attgctttta accgtcgtga ttacttccgc aaaaagtttg aagaagtaaa
      841 cgaaaaaaat taccatgcgt caatttcgtc gggtattgcc aatagtacgt acgtaccaac
      901 gtttggttta gcttcgaatg tgggccagat gattgttatt gtctacggtc tctacctcat
      961 ttcgcagggg agctttacgt ttgggcttct cattagttac ttagcctacg tctctcgctt
     1021 ttacgatcca cttcgtcaaa tggcagcttt gtggtctgat cttcagacgg cattggcagc
     1081 ttgggagcga atcggtgaaa tcctagctat gaagagtaat ttaaccgtgg ttaaggccag
     1141 cgaagatgcg aagggtaaag gcattattga atttaaaaaa gtcggatttc agtatccgga
     1201 tggcgctcct gttctgacaa atatcaactt tgtgatgtat cctggcaaga cgtacgcatt
     1261 agtgggacct acgggcggcg ggaaaaccac gacagcgtct ctgattgctc gcctgtacga
     1321 tccaacagag ggaacggtct acttgaatgg tcaagatatc cgtacgtatg aagccgatgt
     1381 tcggactgcc aagattggtt ttattttgca agatccattc ttgttcaccg gtacagtccg
     1441 agaaaatatt ctctatggta ataacgcata tgctgagtat accaatgaac aacttgttga
     1501 gctgctcgaa agagagggtt taagcgatct attaaagcga tttgatcaag gactcgatac
     1561 tccggttact acaagcgcgg acggtattag tcttggacag aagcagctta tcgcctttat
     1621 gcgtgctctt ctacgtcacc ccgatatcct tatcctcgat gaagcgtctg caaacatcga
     1681 tacggtaaca gagcagattc tggatgagat cttaaaacgt cttcctgctc aaaccgcacg
     1741 tgtcattatt gcccaccgac tcaatacgat tgagaacgcg gacgagattc tctttgtcaa
     1801 cggagggaag attactccag ctggttctct cgatcatgcg gtcgagctcc tgacgagtgg
     1861 tgtaaaatct agttagaaga ggattgtgtt cccaaaaaaa taaccgtaga cacacgaatc
     1921 tacggttatt ttttataatt tcaaggggtg cgttagaacg gacggtggcc tccacgcatt
     1981 cctcctcgat ggactccttt gaatccaagt ccccgaaggt actgggtatc aatgccatta
     2041 tcttttgccc actgctcgag tgcgtcacgc tgttttttca tttctgcctg acgctcttgg
     2101 ggagtcatgt ttttgagcgt ttctttcgtg gcttccatct gtgtttgaag ctctttttgt
     2161 ttggcgataa tcttttgttt ttgttcttca gtgattttct tgttggtcac tgcttgggtg
     2221 agcttatcgt caatacgctt ctccatcgtg gcctgaacct gagatttgtg ctcggtgaaa
     2281 acctgttcaa cgtcgctttc ttttaagtta aacttttctg aaagcctttg tacaagcgtt
     2341 ttaccgtcac tggccgctgt atcatgagcg agcgtatgca ttgctccaag tgatacgact
     2401 ccaagggctg caagcccaag aattgggaag agatactttg ttttcattgt actcaccacc
     2461 tttcttactc gctaggttac cagccgaaac tgagaatttg cttatattaa gagactaact
     2521 ctttaatatg gccggcatgg tgaattaact tgccatcttt aagctgaaaa gtcatatctg
     2581 tgtgtttcgt aatgttaagg tcatgtgtta ccacaagaat agttgtttgc tcggttttcg
     2641 caaggtggcg aagaagctcg acgatcattt tactcgtgtt gctgtctagg ttgccggtcg
     2701 gctcatcagc taatataagt ttaggtttgt tggccagggc tcgagcaatt gaaaccctct
     2761 gttgttctcc gcccgatagt cttcccggcc ttcggtgttg tttgtcgtca gtgaggccaa
     2821 cttcgtttaa gagatgttcg gctcgttctc ggcgctcttt tgttgggagg ccggcaaatt
     2881 ccatggggag cataacgttc tcgagtgcgg ttaagttagg aataaggttg tagtgttgga
     2941 atataaatcc aatcttttct cgacgatatc gaatgagttt actatcgcca agtttagtaa
     3001 tgtcttgatc gtcaactttt atagatccat ggcttgggcg ttcgagtgct cctagtaatg
     3061 ctaagagcgt acttttaccg ctgccacttt ttccaacgat agcagcaaag gaccctgccg
     3121 gaacctgaaa acttagcgta tcaactgctt ttacttggat gtctgcggcg tgaaactctt
     3181 ttgtgagatc ttttacttca agcatattat tctcctcgta ttacttctgc gggtcgtact
     3241 ttcgcaatga gccaggctgg cagagcgctc ccgataatgg cgattataag tgctgccaac
     3301 aggccataga gtaaaatatc gtagctcatt acggaatgga tatctcgtaa gtttgtttga
     3361 acggtgctcc caagctgaga gatacgcata aatccacctc cgcctggtcc ggcaatcatt
     3421 cttccagctg ttgatccatc ggtagtgctt gagttggcaa caagggcaga aagaatgggg
     3481 ttgctaagga aaaagcctag aatcattccc acaatactgc ccaacacagt cagaaccaat
     3541 gactcggtta tgaattgggc gaccacccgt atatttgagg ctccaatagc cttgagaaca
     3601 ccaatttctt ttcgacgttc tcgtacaatc attatcatgg ttaagagcgt tatgattgct
     3661 ccggccgcga gtgagccaat aaggctgtaa aacgaaatat tttttacgtt ctctaatggg
     3721 gaaagcgctt gcttggctga ttcttcgttc gacgtgacgt cagctttatc ttctcccaat
     3781 ttattcttta tggctgttac ggtggcatcg agattggtaa cagagtctac ggaaaccgtg
     3841 gcgacgctta cttcatctgt ttgtcccgaa agccgctgcg ttgtttttaa aggcatatag
     3901 aggctactat tggcaaattg attgccagca tcataaatgc cactgacggt aattgtcgtg
     3961 ccgaaagcgg taaatgttga tccggtcata aggccatttt tttctgcgag tgattttcca
     4021 aggagcgcga cattatcgtc ggaatcgcca ttaaagacgt ttccacttat taccgaagta
     4081 ctccctgtag taagaatgga tgtgtctttg atgccagtta ccatgatggg aatcgtgaat
     4141 gtacgtgttt gaccttgtgt ccccggagaa atagtaggta catcacgttg gttaaatcga
     4201 ttttgccgta atccaagtgt accggcttcg atagcggtaa ctaatgacgt gtctgttcct
     4261 gcggtcattc gagctgagag agttgccgct actttgttaa catgctcaag gttgcttagg
     4321 tcgtttattt gcgagacagt gagtggctct ccaccacctt caaatccacg ggctccagcg
     4381 ggattaaccg taattgtgtt accgatggtt gattttaccg tttctatacg agccgaaaca
     4441 gttcgataag caagaaacat aatgagggcg agtccgatac taagtgccag aatgagcgtt
     4501 attgaaaaag tacgtatcgt attgcgaaaa gcgtttctgg ctccatgcac gagtatgctc
     4561 ataatcatcc tttcctattt gagggttcag tatgagctta gtgagctagc ctgagaaaga
     4621 gcttagaata tcaatcccgg gaaaagatcg gatagattac ctactcagcg agtcgcgacg
     4681 atggctgaga aataggaagc gttacggtaa aacgtgttcc attttcggtt cgaggagtgg
     4741 catcaatact accatggtgc atttcggtaa ttttcttggc gattgcgaga cccaagccat
     4801 gtccatcggc aatttgttta gaacgagacg catctgctct ataaaaacgt tcaaaaatgt
     4861 gaggaaaatc ggatgcttta attccaatcc cttcatcgtc gatatgaaag attatttctt
     4921 tttgttttcg ttttattgta ttcgatatcg ttactttggt atgagaaggg ctgtatttaa
     4981 ttgcgttatc aagcaagatg gtgaagagct cggtcaaact acgttcgtct ccttcgatct
     5041 cactatttct taccgaatta tcaatcacaa tttcttttga tttggccagc ggggcaattt
     5101 ttgtaattgc agcctgtatg atgtcaccgg tttggcatgt ttcgaaggcg tgtagggctg
     5161 cctgagtggg aggctttgct aacttgagta ggttgttggc taacgtttca agtttgccta
     5221 cctcctccag attgctcgcg tggagttgtt tcgattcttc gaaagtaaga tttttgtcgc
     5281 gtaaagcgac ttcgagctcg gttttcattg cggtcagagg ggtgcgcaat tcatgggacg
     5341 catcggtaat aaaacgatgt tgggcttcaa gagattcttc gattggctca agtgtccttt
     5401 gtgctaacaa gtagctgagt cctccgcccg ccacgagaat aacgacgtta aaataaacca
     5461 gattcattaa aatgtgttgt ttgctttgtg caatctcttc gagtcgtaac cgccgaaatt
     5521 cggtaaaccc tcgaggcgca aagagggttg actgttgcat agactcaaaa cgtcggattt
     5581 cgcgattaaa ctcttgtgtt gatacttgat acagaacacc gctaaataac ccacttaaga
     5641 ccatgaggat aaggaggtac caaagggtta agcgaagtga agccgatcga aagagaggcg
     5701 taccgaatga gttcatgcct tttctccaaa ccgatagcca aagcctctga tagtgtgaat
     5761 aaggggacgg cttggaaaag gcttatcaac tttggagcgg agatagccga tatacacctc
     5821 gacggtgtta gggaggatgt cggcatcgta gtcccataca tgagaaatta tctgatcttt
     5881 cgtgagtgtg cggcctttat tacgcattaa gtactctaaa agagcgtact cttttgttga
     5941 aagagaaata ggctttccgg ctcgagttac ggcaaagtta acggtatcta cgcggaggtc
     6001 atcggttttc agcagggagt gggttgtttc ttgaggacga cgaagcaatg cgcgcacccg
     6061 agctaagagc tcttcaaaag caaaaggttt tactaagtag tcatctgcac cagcgttcaa
     6121 gccaccgacc cgatctctga cttgatcttt tgctgtcagc atcaagatag gggtgtgtaa
     6181 gcctttatca cgaacgtttc gacaaatttc catgccatcg gttatgtcgg gcagcatgcg
     6241 gtcgagaata atcaggtcat aactttcatc gagcgcatag ctcataccct gttttccgtt
     6301 gtaaacaaca tcaacggcga aggactcgag ttcgagtcct ttttttaagg cgttggcaat
     6361 tttatgctca tcttcgataa cgagtaatct catataggta tagtataccg cgatactgag
     6421 aaaaaactga aatttttacg cctcttttta ttacatactt gacatcaatg taccaagttg
     6481 ctatattgtt gacatgtcaa ataaaagcat tcttcttacc cccgacacaa gtttaaagct
     6541 tgttataagt atggcacgag cgcagtctat tatctcccgc tactttgatg gtagattggg
     6601 aggtggaatc ggccttaacg attttttgat tctttactac cttactagtg cggaaggtaa
     6661 taagttacgt cgtgtcgact tagctcacaa gatgggtttt actccttcag gagttacccg
     6721 gctcttagct ccgatggaga aaattggttt aatcaaacgc gaacccagcc aacacgatgc
     6781 tcgggtaagt tatgtatcgc tagctccgag tgggcaacga atcctgcagg aagaatttga
     6841 gcgagctgag ttgcttgccc aagaagtctt gccttcctca cacgaagtag gcgactttga
     6901 aaaattcctt tcagtgattg gaaaagtaac ttcataatca aaataaaaaa tagctcctac
     6961 gagctatttt ttattttagg attagagttt acctaagtct aatgtttcgg tgaatttaat
     7021 ttgttctaca aattccggta cgtgacttac taagacaacc gcgccttcgt aatcctgaat
     7081 agcttgtgca attaccggaa tatggcggaa gttaatatga ttggttggtt cgtcgagtat
     7141 taagagacca ggttgttgaa gcgtaaatcg agcaaagcag agtaatcctt tttgcccctc
     7201 agacaagctg ccgactttgt ttttgagtaa atcagatgta attaagaagc gggctgcagt
     7261 gcgataaatg gtttcattat ctgctgccgg atccatttct tctaaacttt cacgcacggt
     7321 agcgtcaaag tttaagccag aaaaatcttg gcgataatag cctactttaa cttcggggtt
     7381 aatttttgct ccggcttgag tgcctcgtgc cagtgcttcg agtaaggtgc ttttaccaat
     7441 gccattggac ccctcgagga gtaagcgttg tcgcttacgt aactgtatat caacgggttt
     7501 cgtaaccggt gtatgatcct ttaaaaacgt aactgagtta attgtaacga tcgcatccgg
     7561 gaagtcctgg aaaggaatgg taaagtgacc taatgttttt tcttctcgac gaacttccgc
     7621 tttgttctct tctgcttctt cgatagaatc gcgaagtttg ctggccaatt tacgcatctt
     7681 tccaccttta ttggcgaaaa agttcacttt atctttgttg tcttggattt ctttctcaag
     7741 ccgtgcattc tggcggcggt cgcgttcaat ctgtgcttcg attgcatcaa ctgcggagta
     7801 gtagtcgcca aggtactttg ttactttgcg gctaaataca tctaagtgta gaacgccgtc
     7861 agtgaacatg ttcaaaaagt tggcatcgtg ggaaattaca atacaggttt taggatagtt
     7921 aattaaaaat tctgtcagat gccaaatacc tgtagggtcc agattgttgg ttggctcatc
     7981 gagcaaaagg atatctggct cctgaatgag tccgtatgcg agtaagagtc gggcttgctg
     8041 gccaccagaa aactcgttca ttttttttgt gagaggagca acgagattaa ctgtttctaa
     8101 tacgcttgta atgcgacgtt caagatcccg aggtacatct ttaaaagctc cggcaaaaaa
     8161 ctcttggaca gtcacattaa gtttatcgcg aggcacaacc tggagtgatg tggcaatctt
     8221 tgcatcgtct cgaatattaa tccggccgga ttggggtttg agttggcctg caatgagttt
     8281 aaagagagtg ctttttcctg ctccattttg tcccattatt gtgaatttag ctcgctcgcg
     8341 gatgctaaat tcaacctcat ctaacagagg tttgagaggg tcgtacccaa aggatactcg
     8401 atcgaagctg ataataacgt caccttgagc catagatatg tattatacct gtaaatgagt
     8461 ataatttcaa aatagtaaga tggaatgaca ggggaagatt acttttttag tgctggcagt
     8521 gcttcggcag tttgacgaat gaaattacta agccagtctg catcgtccca cttctctgaa
     8581 gatacctgta agtacatttt tgcgttaggg tacggaggtg cttcgtggta gaactcggaa
     8641 aaatgtgaac ttattgagct ttgtttgacg aaaagctgat catcgcacac cagagcgaca
     8701 actttgtcgt tacaatagag ggcatattca ccaaacattt tacgtgccct tacattgcct
     8761 gctgattgaa tttgttcgag aagaaaatca atagttgttg aatgagttgc cataaaacta
     8821 ctttttactc agtatatcaa acgcaacaat ctggtatact gattcgtatg gtatacattg
     8881 ctttcttacg ggggattaac gttggtggtc atacggtaac tatgcaacgt ttacgagaac
     8941 tttttgaaga gctcggattg caaaatgtac gtacgtatat ccaaagcgga aatgtctttt
     9001 ttagtacttc cgaaacagac cgggttaatc tctctcaaat aattgagttg cacttacggt
     9061 cggcgttagg ttatgcggtg cctacctttt tacgtacatg cgaagaagtc gcctattctc
     9121 ttgaggtttc tccgtttaaa gggatagagc agcccgagga taagcggttt tttatcatgt
     9181 ttctttcgac agctgtctct cctttaactc tgccaattgc ttcgccgaaa aaagactacg
     9241 aaattgtggg acaaacagaa acagagcttt ttgtaatttt ggatttaagc gttgggcggc
     9301 cgggaaatcc caatgtcttt attgaaaaaa actttcaagt tcaaacgact tcgcgttttt
     9361 atcatacaac tcgaaaaata ctcgatgcag cccagtctat ttaagtggtt gacaaaagta
     9421 agtatataag ataagatgat ctatgcattg ccaaaccgcc ctgtatgggc gaatgaatgg
     9481 ctcattctct agaactcttg catagtagat gctaacgagt tctctacgtt agcactgcca
     9541 ctactattca tatgaacctc ccggatcaaa gtatcctgta agccataggt tcatacattc
     9601 ccgtggttat ccacggataa aggaatctat gtataatcgt tctcctcgac cgtttggatc
     9661 gagttctcag cgtttttcac gacgcccaaa tcagcgcaaa tttactggca aatacatttc
     9721 gattgaccgc tttattcaaa aagcttcaat gacaacacct gtcgaagtgt atgtacccaa
     9781 acataacttt gctgattttt cgctcaataa aattttgaaa gcaaatatat taggtaaggg
     9841 ttattcaacc ccaaccgcaa tccaggatcg cacgattccc gttagccttg aaggacgtga
     9901 tgttattggt attgcgaata cgggaaccgg taaaacagcc gcttttctca tccctctgat
     9961 tcataaggtt tcgaaccata aatatgaccg cgtcttgatt atgacgccga cgcgtgagtt
    10021 agccttgcag attcaacaag aatttaaaga gtttgctcgt ggtatgaacc aatatgcaac
    10081 acttgttatt ggcggtgcac caattggagc gcagattgct cagctacgtc gtaatcctca
    10141 gtttgtcatt ggaactcctg gtcgcttaaa agacttagtt gaacgccgcg tcttaaacct
    10201 atctggtttt cgctcggtcg tactcgacga agctgaccgt atgttagaca tgggctttat
    10261 cgatgatatt cagttgctct tgtctggtct ttcagaggag cgtcagaccc tctttttctc
    10321 agctactatg tcgcctcaga ttcaggcttt gacacgtaac ttcttgaaag atccagttat
    10381 gatctcagtt aaaagccaag atacgtctgc caatgtagat caagatgtta ttcacgtgaa
    10441 tcacgaagat gatcgtattg agcaactaca cgagttactt gttcagccgt cgtataataa
    10501 ggttttgatc tttggacaga ccaagtatgg tgtcgagtcg ctcagtaacg agttagctga
    10561 ccgtggtttt cgtgtggcgt gtatccatgg cgataaaacc cagccaaaac gtcagcgtgc
    10621 cttaaatatg tttaaacaag aagacgttca gattcttgtt gcgacggatg ttgctgctcg
    10681 tggtctcgac attcctaacg tgtcacacgt tattaactac gatatccctt cgacgtatga
    10741 agactatgtg catcgaatcg gccgaacagg ccgcgcaaat aataaagggt atgctcttac
    10801 gttcgtgact gcccacgccc agcccagtca taatcgctac tcacgttaag gtcagaaacc
    10861 aaaaaagagc tcttggaagc tcttttttgg tcatcagttt tttaaaaaga gaatataaaa
    10921 gtacaagtcc gggttgatgg aatcgaggtt acttacgaaa gcgattgctt ttgtaaattg
    10981 taaaagtgta taaaaatagt gaaccaaagg ctgcttgttc gcgctctttt gcccatgcgt
    11041 tcatgcttgc cggcgcatac aatgaattca tgatgacacc acagaaacga ctcacccttt
    11101 ctcagcggtt ctt
//
LOCUS       DASWYB010000011        26487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_315690, whole genome shotgun sequence.
ACCESSION   DASWYB010000011 DASWYB010000000
VERSION     DASWYB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26487
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315690"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VGE59_00615"
     CDS             complement(<1..439)
                     /locus_tag="VGE59_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074193.1"
                     /translation="MQQLTMPPTRHPAHVLHVSLLQPGAAPVSASFPVDPGRPYQQQI
                     RNTIGPLPGARAQLLKVQIGETETQVSGIEVECDNDVMQARWFSFRWSAVVLITRGEE
                     VIAREYRVPTRTVRVLPYLGRKVQDEYPDAKSFQVFSLSSLCEK"
     gene            complement(701..2166)
                     /locus_tag="VGE59_00620"
     rRNA            complement(701..2166)
                     /locus_tag="VGE59_00620"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(2341..2412)
                     /locus_tag="VGE59_00625"
     tRNA            complement(2341..2412)
                     /locus_tag="VGE59_00625"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(2484..2556)
                     /locus_tag="VGE59_00630"
     tRNA            complement(2484..2556)
                     /locus_tag="VGE59_00630"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2521..2523),aa:Cys,seq:aca)
     gene            complement(3168..3656)
                     /locus_tag="VGE59_00635"
     CDS             complement(3168..3656)
                     /locus_tag="VGE59_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEY1074194.1"
                     /translation="MESNKVTVTNLVLLVNYDEQKILLAMKKRGFGAGRWNGYGGKVK
                     GEESIEGSAVREIQEEANVALQEEDLKKVAHHSFYSPAFGTVEVHTYIAEEWEGEAVE
                     TEEMRPEWYSFKEIPYKDMWQDDKYWLPEVLKGRHLRGEFWFDKDDNLLKYTLNDYIF
                     TA"
     gene            3755..4087
                     /locus_tag="VGE59_00640"
     CDS             3755..4087
                     /locus_tag="VGE59_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074195.1"
                     /translation="MLPWPEDPNFAREIDDWIFGEEDEGEVLNDPSMDLFAATAEELY
                     YETEFALYWEDQQRNQVAKFQRMIEVATVIAQITYLYEYRPEAEGKMHQWPTQVYCFV
                     PPDPPILN"
     gene            4211..4723
                     /locus_tag="VGE59_00645"
     CDS             4211..4723
                     /locus_tag="VGE59_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074196.1"
                     /translation="MPEDVCIVTVLVDLPFEEAECLANRYLPPIACMWKWRSNGQKLP
                     QLVISAPLLGYLLSSRIARRIVEQLPQEKILEIFFIREVTQEEMRSGAIAQNVYGRRE
                     VIVGSLGRSKLTPRDTEWGHLYTGRGMPLPFGRRNEEVDVIEVRAPSLSMIDQLIQKW
                     RDGGILSEDP"
     gene            complement(4798..5391)
                     /locus_tag="VGE59_00650"
     CDS             complement(4798..5391)
                     /locus_tag="VGE59_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074197.1"
                     /translation="MLQECVECTVVFLMRNNRVLLARGKKRMNKGLWNGYGGKKDPNE
                     TIRRTACREAAEEGGVLIQEMDLVYLGVMDFCWPTQGADVGVKGVKVVRSYMFVAHNW
                     EGEPIETDESECPTWYDPEKVPYDTMFPAEQCWLPFLLAGRHFLGSVVYAQKRKWALK
                     FKKSKWGVPVLTVRENTVEVLSDTVWEVPREALPPLR"
     gene            complement(5486..6103)
                     /gene="clpP"
                     /locus_tag="VGE59_00655"
     CDS             complement(5486..6103)
                     /gene="clpP"
                     /locus_tag="VGE59_00655"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY1074198.1"
                     /translation="MDAHNQILVPTVLEKTQFGERAYDIYSRLLKDRIIFIGTPIDDH
                     VANLVTAQLLFLESEDPKKDVTIFINSPGGSVSAGLAMYDTMRYIKPDVSTICIGMAA
                     SMGAFLLAGGTKGKRYSLPNSRIMIHQVMGSVGEHAQATDIDITAREILRLKQLLNEE
                     LSANTGQSLEKVTADTERDFWLSAPAAKEYGIIDAVIERNPAPSK"
     gene            complement(6413..7633)
                     /gene="tig"
                     /locus_tag="VGE59_00660"
     CDS             complement(6413..7633)
                     /gene="tig"
                     /locus_tag="VGE59_00660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY1074199.1"
                     /translation="MQLTIEKLQNSRMKIIVKAPAADLAHAAEHALHHLSAQVAVKGF
                     RPGKAPKPMIVAQLGKGRILSELVDHALPELLEEVVRKEKISLIEAPSYTLEKLCELN
                     DDGTVKEDSTLEFTAEADYAPDVKVGDYTKIKIKPAKVAEVTDTEIDSVLTDLAERRA
                     TYDTVERAAKEGDRVEIDFAGKRNGIPEDRLASKHYPIVIGSKTMIPGFEDQLIGKKA
                     GDAFSFEITFPKDYHAKDMAGEKVVFEVTVHEVHERKLPEINDDFAKEFEQESLDTLK
                     AAIRQEREYSFGEEAKQADEAATLEEFLKLVEVDVPQSLVEREIDRQVEALKQQAAQF
                     GLNFEQYLQHMKKTEEEIRKEMRESAEKAVKIGLGLGEVVEREGLEKDKNAGYVAIEK
                     LVEIATAKTASKKK"
     gene            complement(7712..8872)
                     /gene="pgk"
                     /locus_tag="VGE59_00665"
     CDS             complement(7712..8872)
                     /gene="pgk"
                     /locus_tag="VGE59_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY1074200.1"
                     /translation="MESNSEPLVVPSLKDIDISGKRILLRFDGDVPVNIDGTLADTYR
                     LDSVMDTILYCIDKKAQLTLLAHRGRPKGKVDPALSNQILADYIAAAVKQPVSLVTEM
                     LGPMPATPILMLENLRFHSGEESNDTGFAKLLAKFGEIYCNDAFAVSHRAHASVSALP
                     GLLPHVAGFQLAKELQALTPLRDYGEAPYVAILGGAKASDKSPIIADLLDKVDVVVIG
                     GLIAVTYLAAMSQPTGAHKVDPEQVKFAQECICRMAQDDIPFVVPVDYINQHGEIKPM
                     YALTGEELMLDIGPETVKHFDTYIHKANTVFWNGAMGKSEEPQFSRGTIGVAESIGRS
                     GAETRVSSGGDTVGAIRNHNLQHYFTFLSTGGGASLEFLAGRELPGITALRA"
     gene            complement(8845..10059)
                     /locus_tag="VGE59_00670"
     CDS             complement(8845..10059)
                     /locus_tag="VGE59_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1074201.1"
                     /translation="MQTQPIVIIGGGFAGLRTALDLDRLLGHRLDCPIFLIDPSPVHV
                     YTPGLFDLMHPASPRVSVIPFRDILAGTHIHHIQERVVRIDVERKQVQTEHRRFSYAD
                     LVLTPGSDTVPLPQKGAGQSSLSAHNFEHLIRLREQIQANFSRDKERHLHVLIAGGGL
                     SGVELALGLQSYIESQAARQGASSRGCHITLAEESSRILPMFKPAVSHVVARWLKQHK
                     ISIVTKTKLTPSGARRLETESEDGPIKTVVWAHGRQPSLLAAKTKGLRHDAKGRVLVN
                     AHLEALDEPGVWVGGDSAAVDDSGWKASAIAHGNHIARAITSKRTGRTVSPYLAHTPT
                     AIIQFSPDYAMLVKGSRLSAGISIAWLKQWDDLKYYLSILPIPLAFARWAKRGEQLGG
                     EDSLWKVTQSPL"
     gene            complement(10069..11103)
                     /gene="gap"
                     /locus_tag="VGE59_00675"
     CDS             complement(10069..11103)
                     /gene="gap"
                     /locus_tag="VGE59_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY1074202.1"
                     /translation="MGKIAINGFGRIGRVAFRILYERGLHKNIVAVNDLTDAANLAYL
                     LKYDSNYGVLPADVKGVVVENRAPVTGEISVDDHTFSVTSVKEPQELPWKDLGVDVVI
                     ESTGRFTDEIGMRKHIAAGAKRVILSAPVKDDTVTTVVRGVNEDALGDQELVSNASCT
                     TNCITPVAAVIEEKFGIEKAMMTTIHSYTASQAIQDAPNKDYREGRAAAQNMVPTSTG
                     ATKAAAKALPALQGIFLGLSVRVPTAVGSLADFTFVTKKDTSVEEVKAVLQEAGRQAK
                     YKGILEVTEDQIVLTDIVGNPASAIVDLSLTQVVGGNLVKVVAWYDNEWGYANRLVDQ
                     VVAMAGLGKE"
     gene            complement(11114..11305)
                     /locus_tag="VGE59_00680"
     CDS             complement(11114..11305)
                     /locus_tag="VGE59_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074203.1"
                     /translation="MVQVNAWKQSLWVAIGWFIGSFAYKLLLHPEESLARRFVDSIGP
                     ALLLFVLSVVVYVMLSRRK"
     gene            complement(11330..12079)
                     /gene="tpiA"
                     /locus_tag="VGE59_00685"
     CDS             complement(11330..12079)
                     /gene="tpiA"
                     /locus_tag="VGE59_00685"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY1074204.1"
                     /translation="MKRMVIGNWKMNLTLPEAMALAQGSIKAAEKCHGLEIAIAPSHV
                     WLMPIKDSVRMLPPNFSLVSQAVSQWPEGAYTGDIAAKQLKGLVTYSLVGHSERRQYH
                     HENGKVVSEQIKQLLNQGITPILCFGEMGQATQHTFPPQITIDLRHDLEGLKPEEIKQ
                     CIFAYEPLWAIGTGNPASPDYVAKAIVHVKNWVSEKYGVHVRIIYGGSVTEDNAGALG
                     SIRELDGLLVGGASLQIKRFSQICSDFCSIV"
     gene            complement(12136..12339)
                     /locus_tag="VGE59_00690"
     CDS             complement(12136..12339)
                     /locus_tag="VGE59_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074205.1"
                     /translation="MASRLGKVERALYIVASFLFLTSAGITFFDYMSISEEPHSALNL
                     VYAAGILVIGFFFFKLGTRREKK"
     gene            complement(12345..13796)
                     /gene="proS"
                     /locus_tag="VGE59_00695"
     CDS             complement(12345..13796)
                     /gene="proS"
                     /locus_tag="VGE59_00695"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY1074206.1"
                     /translation="MDENTVKQSSSRITPRHEDFSEWYHDIIAQADLAEHSGIKGSMI
                     IKPYGYALWENIQKVLDAKIKETGHENAYFPLLIPESYLKKEAQHVEGFAPELAVVTH
                     AGGEKLEEPLVVRPTSETIINASFSRWVQSWRDLPLLINQWANVVRWELRPRLFLRTT
                     EFLWQEGHTAHATQQEAQEEQKKMLDVYTDLAENYMAMPVIDGEKSAAERFAGAESTY
                     TIEAMMQDGKALQAGTSHALGQNFAKAFDITFSDKDGTTKHVWTTSWGVSTRLIGGLI
                     MTHSDDDGLVLPPNIAPTQVVIIPIGRESDLEQVVTAANNVAEKLKQAGVTVRIDTRD
                     NYTPGHKFNEWEKKGVPVRLELGPRDLAADQVVVVRRDTKDKETVGLADVTAKVKQLL
                     EDIQKNLYYQARLRRDERTFTIDDYATFQERIEHGAFLYAHWCGSEECEKQIKDETKA
                     TIRCIPFDQPAETGTCIKCGGASHKRALFARAY"
     gene            13864..14388
                     /locus_tag="VGE59_00700"
     CDS             13864..14388
                     /locus_tag="VGE59_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074207.1"
                     /translation="MKEEILLTEYEILRREVIERVGALHRLIALASILAFLSLLGTTF
                     LFSFHATQTQLWTYLLFLPPVFAGITFNYQANQMTLEAVAMFSDKIRKQLGGNAMGWD
                     GFYGRWKKAVQLTSFLKTLPLLMPLAIPFYALSQEQLLKSEPQLVWLTIFDIVLATLV
                     VINFYYKLLRKVAA"
     gene            complement(14391..15503)
                     /locus_tag="VGE59_00705"
     CDS             complement(14391..15503)
                     /locus_tag="VGE59_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY1074208.1"
                     /translation="MLITFLVFIFILGLLVFVHEFGHFWVAKRSGIKVHEFAFGFRPR
                     LFSWKRGDTEYAINLLPLGGYVRLEGEDQDPSQAKKTKDSFAAQPVGVRAAVLVAGIV
                     MNLVLAWALLTATYSVGSLPLSPSFAEHPGLDIQPVIILADVTVGSPAQIAGLRPGDT
                     ITAINGESLTSVQSLVSTIQSQAGKEISLTYIRDGRTVEVHATPRENPPVGEGALGIA
                     PQEVSHVRAAWYRAPFIAIREVGAEIKGSFLGFIGFVQRLVVKQEVSEDVSGIIGVGA
                     LTGTVRRLGVGPLMQFMALISTNLAVINILPILPLDGGHLLFVGLEALRRKPVPEKYR
                     QWVALAGLAAIGLLFVIVTYQDLLRFSIFDHLKSLI"
     gene            complement(15594..16148)
                     /gene="frr"
                     /locus_tag="VGE59_00710"
     CDS             complement(15594..16148)
                     /gene="frr"
                     /locus_tag="VGE59_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY1074209.1"
                     /translation="MTDILSDMKKKMQDVMEHVNHEFSTIRTGRANASLVDTLPVQYY
                     GNTTPLKNMASISVPEPTMILIQPWDRQSIGDIELAIRNSDLGLSPVNEGSQIRLVLP
                     ALTEDRRNQLVKTISQKAEAGKIALRNVRKDAWDEVQKQVKSGELTEDDRYRYEEDLN
                     KAIDTFNKQVDDLVEAKERELKTL"
     gene            complement(16259..16720)
                     /gene="tsf"
                     /locus_tag="VGE59_00715"
     CDS             complement(16259..16720)
                     /gene="tsf"
                     /locus_tag="VGE59_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY1074210.1"
                     /translation="MATFSMDTLKQLREETGFGIMDIKKALEQADGDMAKARDLLKEK
                     SAMVVAKKADRETKQGLIHTYAHLGKIGAMVEVNCETDFVARNDDFKTFVKDLTLHIA
                     SMNPADLDELMSQPFFSDEKVTVRQLLDQMVGKIGENIQIRRFARFELGAE"
     gene            complement(16707..17474)
                     /gene="rpsB"
                     /locus_tag="VGE59_00720"
     CDS             complement(16707..17474)
                     /gene="rpsB"
                     /locus_tag="VGE59_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY1074211.1"
                     /translation="MHPMTIQELYNVGLHYGHKKEHSTPMTRPYVYSVRDGICIFDLE
                     KTLEHLETALEAVKTAAAQNKKVLFVATKKQARGLVTALAKSVDMPYVTNRWPGGMLT
                     NFTTIRKSLDHMLDLEKTAAAPEFAKLKKKEQKRINDEIAKLHRSFDGIKDMTNLPDM
                     IFIIDGKSEQIAIEEANKLGVTTIATIDSNGDPTKVDMFIPCNDDAPRGLKYVLQRVG
                     EAICEGQGIKFEAPEELSVDTSELTTVKKVGTTNGNL"
     gene            complement(17614..17814)
                     /gene="rpmE"
                     /locus_tag="VGE59_00725"
                     /pseudo
     CDS             complement(17614..17814)
                     /gene="rpmE"
                     /locus_tag="VGE59_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            18063..19184
                     /locus_tag="VGE59_00730"
     CDS             18063..19184
                     /locus_tag="VGE59_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074212.1"
                     /translation="MNRSLFRGALALLITAVTLVSAAHAGGPRETLLLPANYQVANLT
                     EDGLGVGAERIGHGRMTSSKPSLFYLAGKKAKSWPYRPQGPGQVYGFAIARDDAGNVL
                     ASVSRDRRGNEWTVVINSSDGSFRPLPPNFIPATMSPSGVIVGTGIDFILEKIYPVRI
                     LRDGTVEYWEGEDAWAVDVTLTGDVLIQLPGEYDEDGNLSKNPVIEYWKADGSVQGVG
                     EGYATACSNSGTYVVADQDGDQVRFNCSTYVDYSAASTFLAPLEPNGSQVNGTYAADV
                     NDTGEVVGSSNGRAAYWPGTISGGVIPLGGPVLRGITEATELGAMIDLPRGYTAQGGY
                     RITGSGAMLVGITRGTGRKAEYRTLYVRPTPIDLPRPLQ"
     gene            19363..20388
                     /locus_tag="VGE59_00735"
     CDS             19363..20388
                     /locus_tag="VGE59_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074213.1"
                     /translation="MRNQLFHRALMALITLSVCAAGFVAAAPESRIVQRKVVTLPKSL
                     MLSKVNDQGVGAGASNPLRPTPVTAKLSGTSAKIHRLTPQNGLAYSLNNAGVSVGVVL
                     NGEKEVPVVFRANGATEPISTAVTVWPSHINDSGIIVGEIDGATYNTSIAARVIAGNV
                     EIYLDTRPMEPGYRTQGSTAIDIASDGTTLVQLYSVRNKVGIEVLPRQVILWHTDGTS
                     TPIAEDAWGSAISDNGRFVVGIQQASPIRWEISGSAVTTTHVTEPSGATYTYGIDINN
                     TGAMVGEAEYTAIYWPTPGEPIAVTQMARFKKSVQALSAISLTNKGGILVQCRKGTTY
                     FQSYLRP"
     gene            20533..21531
                     /locus_tag="VGE59_00740"
     CDS             20533..21531
                     /locus_tag="VGE59_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074214.1"
                     /translation="MITTRNRWLLLIALASLALALPGQTATAKYTFMPLPPLFTANDL
                     SDQREIVGSTRDGAIGSDEEYATSYLASIAGLIELDQLSEPSVYRGISPSGDHIAGWS
                     KNTLGVWVGQVWSWAIPSYSTGPNTFPVAVNNSGRSTGVQVTWGPNNQIATQKVFRSP
                     ATGTTLEILDEWGDPVGISTGGIATYTLTEVLGGRVINRVKNWLPNGSRIVIDEGECF
                     GVSSSGATIAGQKLTQATLWTASGAMNLKAGTNSLALDANDAGTAVGISRGRAYLFER
                     GKRVDLNTMVKLPKEWKKWRLVRAKAINNRGDILVTAQNLTPSQGKPAYVDVALLK"
     gene            21625..22521
                     /locus_tag="VGE59_00745"
     CDS             21625..22521
                     /locus_tag="VGE59_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEY1074215.1"
                     /translation="MINQKLISELRDLETMKQTGSDPEFLTMVEEEMTKIRERIFAED
                     PDNTRPLILEVRSGTGGEEAELFAADLLRMYLRYAEIKNWKTDIVEMNESSIGGVKLA
                     VVGIRGFGAYPLLKLEGGVHRVQRIPKTEKAGRIHTSAASVVVMPEVEEREFDIRPED
                     IRIDVYRAGGKGGQGVNTTDSAVRLTHIPTGMIVTCQDERSQLKNRDKAMKVLRSRLK
                     QLDDERQAQAKGDLRRSMIGSGDRSDKIRTYNFPQDRVTDHRINQSWHSMSRIMGGEI
                     DTITQTMRTTELNEILHAEDSA"
     gene            22521..23372
                     /gene="prmC"
                     /locus_tag="VGE59_00750"
     CDS             22521..23372
                     /gene="prmC"
                     /locus_tag="VGE59_00750"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY1074216.1"
                     /translation="MTLHTWLKNATTRLEAQNISSSRIDSELILAHILEKDRAYLIAH
                     VDDTLSSAEEAHATELLERRLKNEPMAFLLNSREFYNLTFKTDRRALIPRGESEPLVE
                     TAIEWLQNRPESQIVAEIGTGSGALIISVAKAVPTHTFLATEISPEALSLAKENAELH
                     QASINFFEGNLGEPLLTDYAGSIHLLMTNLPYIATDLLTNLDPTVTYYEPNLALDGGG
                     DGLNLYRQFLPHAKQLLTSGGLLLCEHEHDHSEMMRELVREQFPDAVIKTVQDSLGHD
                     RLLVCQT"
     gene            complement(23369..24424)
                     /locus_tag="VGE59_00755"
     CDS             complement(23369..24424)
                     /locus_tag="VGE59_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY1074217.1"
                     /translation="MIQSFKNRFEPHLRDYVRTKIRESQALIRDDFLKEQIESLEDLV
                     MAGGKRVRPYLIDLMYRSAGGTQQCPYGPLIGMELFHVFCLIHDDVMDQGTSRHTVPT
                     FHIQTAQALKAQQRPGDIHRVSESQAILIGDLVHSWAHSCFLQVETEDMAQVYEVNEL
                     VSQMVSEVILGQMIDVDSTTLDVASKDMIERKMNLKTARYTFVGPMKIGRALATQSKA
                     LDQFCEQFGTSLGLAFQIQDDLLDLVTPSTESTKSVLSDLAQRQHTLFTYYIFNHGTA
                     QEIATLRRYFGQPCTDGEEAHIQTLFKNSGAIEYGLTRIRELLAQAQSALNEAPLEET
                     YREQWQELLNYVSERKH"
     gene            complement(24427..25560)
                     /locus_tag="VGE59_00760"
     CDS             complement(24427..25560)
                     /locus_tag="VGE59_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY1074218.1"
                     /translation="MQTNFERQKLVALALACGLLAGVLGGSATLLSLSVLNIPGLSNP
                     NGGITSTTTKKERVVLEESSAVIDAAKKVSPSVVSITTSRNIQDFFSGRTVQEKGGGT
                     GFILTSDGLIVTNKHVVSEGDTEYTVLTSDGKSYTAKVLSTDPFNDLAVLKVDAKGLK
                     PVELGDSNALQVGQWVIAIGNALGEFQNTVTVGVVSAKDRRITASDEVLDSLLQTDAA
                     INPGNSGGPLVNLAGQVVGINTAIAGGAEGIGFAINIDSVKTALDSVQKTGKIVRPWL
                     GVRYLSLNKEIARSAQLPVDHGALVYRGTNPADLPVVPGSPADKAGLAENDIITQING
                     QEINENNSLVSLLQKYKVGEKVTLTVLRKGEEKKIDVLLEEQK"
     gene            complement(25571..26104)
                     /locus_tag="VGE59_00765"
     CDS             complement(25571..26104)
                     /locus_tag="VGE59_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY1074219.1"
                     /translation="MTDFFLTIDVVAPLLEHVKEAIVRLPGALITLFVSYCFLKLIQA
                     IVAASLRRTKITKPMQGILLSVLSVVLWVGLFSLVFQSLGLNQIAIAISSSLAVIAIG
                     VATGANKVVADISAGVYLARSNDFKIGRQVKIGDLEGQIYSLDSRKVRILKKDDTLAI
                     VPNSKFDELVWEVPKGE"
     gene            complement(26187..>26487)
                     /locus_tag="VGE59_00770"
     CDS             complement(26187..>26487)
                     /locus_tag="VGE59_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074220.1"
                     /translation="VASLASPARFSGWLRRETPQAVLISARLSQSNIEQVARTLRLKY
                     PTRRIPLIVVANDEVPNSLLAIKGVGEAFCLHRISLREALDRLTLAIQLTQISRV"
BASE COUNT         6681 a         7129 c         6063 g         6614 t
ORIGIN      
        1 tcttctcgca caaggaggag agggagaaca cctggaagct cttcgcgtcc gggtattcgt
       61 cttggacttt ccggccgaga tacggaagaa ccctaacggt acgggtagga actcgatact
      121 cccgagcgat cacttcttct ccccgggtaa tcagtacgac ggcagaccat cggaaagaga
      181 accatcgagc ttgcatgacg tcgttgtcac actcaacctc aatgccacta acctgcgttt
      241 cggtctcccc tatctgaacc ttgaggagtt gggcacgagc gcccggcaat gggccgatcg
      301 tgttacgaat ctgctgttga tacggacgtc ccggatccac cgggaaagaa gcggaaaccg
      361 gagctgcgcc cggctgaaga agcgatacgt gcagtacgtg tgcaggatgc cttgttggag
      421 gcatggtgag ctgctgcatt cttctctttt cccatacgta acggccgcca ctctttgacg
      481 acttatagac tattttactc gtttttcttg agccaagcaa gccctaaact agttaaatgg
      541 ccatttttta agaaaacaaa acgtcccgac ttgcgtcgag acgttttgag ttaaccgaat
      601 ctctgtggtt gtaagaaatg aagtgcataa atctttttgg tataaatacc tcgaagaccg
      661 acctagccac ttgtataaat acaagagcta atccttttag aaaggaggtg atccatccgc
      721 agattcccct acggatacct tgttacgact tagtgctcct tgctgaccct accgtgttcc
      781 gaataaaaaa tcagaattcg ggcattgcca actcggctca cttgacgggc ggtgtgtaca
      841 aggatcgaga acgtattcac cgtggtatag ctgacccacg gttactagcg attccagctt
      901 catgcaggcg agttgcagcc tgcaatccga actgaggcgc attttgatgg gattagctcc
      961 gtctcgcgac tttgcagacc ctttgtgtgc gccattgtag catgtgtgta gcccaggtcg
     1021 taagggccac gctgacttga cgtcgtcccc accttcctcc cccgaatgag ggcagtccca
     1081 tctgaaaaat ccaacagatg gcgagggttt cgctcgttta cccacttaag ggcacagctc
     1141 acgccacgag ctgacggcag ccgtgcaaca cctgtcattc cgttcccttg cgggcacggc
     1201 cctatttcta gggctttcgg aagatgtcaa gacctggtaa ggttctccgt ttagcgtcga
     1261 attaaaccac atgctccacc gctggtgcga tcccccgtca attcctttga gttttagtct
     1321 tgcgaccgta ctccccaggc ggtgaactta acgcgttagc ttcgctacaa ggagggtcga
     1381 tgctcctgat agctagttca catcgtttac ggcgtggact accggggtat ctaatcccgt
     1441 tcgctcccca cgctttcgtg cctcagcgtc tcatatagcc tagcaagctg ccttcgccat
     1501 tggtattcct ctcgatctca acggatttca ctcctacacc gagaattctg cttgcccctg
     1561 ctatcgacta gtttagcagt aacaacggct ggtcccgagt tgagctcggg gatttaacca
     1621 ttgtcttact gaaccgccta cgcactcttt acgcccaata ataccggata acgctcgggc
     1681 cctacgtatt accgcggctg ctggcacgta gttagcaggc ccttattccc caggtactgt
     1741 cataatcatc cctgggaaaa gaagtttacg acccgaaggc cttcttcctc cacgcggcat
     1801 tgcgtcatca cgctttcgcg cattgtgaac gattcccaac tgctgccacc cgcaggtgta
     1861 tggaccgtgt ctcagttcca ttgtggctga ccatcctctc agaccagcta cccgtcatcg
     1921 ccttggtagg ccattaccct accaactagc tgatgggacg caggcttctc ccaaaccggc
     1981 ttacgccttt accctttcgg gactatggga cattagccaa cctttcggct gggtatttcc
     2041 cagttcgggg caaattccta cgcgttactc acccgttcgc cgctaggcat tgctgcctcg
     2101 ctcgacttgc atgtgttagg catgccgcca gcgttcatcc tgagccagga tcaaactctc
     2161 aatataaaga actcttaccc tccggtagga ggttcgagta ctggttttag aaacacttat
     2221 ttaattgcac agagtcgctg ttttctaata cgaccctttt cttacaacca cacaaactcg
     2281 gttcagtatt cgtataaggg aggagggggc agcgcatagg tcgcgctgcc agggaaacaa
     2341 tgggtaagac aggattcgaa cctgcatgat gctgggggaa aacccagtgc tctgccattg
     2401 agctactcac cctgagcggg ggctggatta ataccagcaa cctccaggtc cagcgcctgg
     2461 tgttctgtat gaactacccc gcaagccacg gaagggattc gaacccctaa cctcttgcct
     2521 tgtggcaagc gctctaccca gctgagctac cgcggcggaa gcgggacaag aacctgccaa
     2581 aaccagggtt ttgtgtaggc gagacggtat tcccgtccgg ttcttgcccg cgtgggacca
     2641 aggaagacag aagtggcagg actatacggc cagaagccgt atccgggttt ggaaggttct
     2701 tatacactcc tggggagtgc cagtgaccaa ccgcaccaat cctacaaaac ttcgttcgcg
     2761 atgtgggcga aggtggaccc gaattcgtcg gtcgcaccca aatgtcaaag cgaactaagt
     2821 atcttcgtct tccttcagca ctcgtgtcgg cgaatccttt acaactcgat ccaacggacg
     2881 atcggctctt ctgggtctaa acaacagtcc cgtctgagtc aattttatgt cgttggttga
     2941 gcgaggtcgt agagtcgcgg cacgagcacc gactatgaga gtataaccct cttcccttat
     3001 acgaattgaa cacgcgattg catagctgcg gtttcgcgta ttcttttttt aagtaaccat
     3061 cagcagaggc cgatataatc gacttctgtg attcactaaa accaatttta cccgacttct
     3121 gttacagtgt caacactttt tatacctgct aatgttcttt ttgcacctta cgctgtaaag
     3181 atataatcgt tcaaggtgta ttttaggaga ttatcgtcct tatcaaacca gaattcaccc
     3241 cgtaaatgac ggccttttaa cacttccgga agccagtatt tgtcgtcctg ccacatgtcc
     3301 ttataaggaa tctccttaaa cgagtaccac tctggtctca tttcttcggt ttcgactgcc
     3361 tcaccttccc actcctccgc tatataggta tgcacctcga ccgtcccaaa agccggacta
     3421 taaaaactgt gatgcgcaac tttctttaag tcttcttctt gaagagcaac gttggcttct
     3481 tcctgtattt cccgtaccgc agatccttca attgattcct cgccttttac cttaccgccg
     3541 tacccattcc accggcctgc cccaaatcca cgcttcttca tagcgaggag aattttttgt
     3601 tcgtcatagt tcacgaggag gactaaattc gtcacggtta ctttgttcga ttccatacgg
     3661 ccatagtagc actctggatt gacaaaacaa gctttttatg gtataaaata cgcattcagc
     3721 aatgacggtg cgactactta gacagaggta ctacatgcta ccgtggcccg aagacccaaa
     3781 cttcgcccgc gagatcgacg actggatttt cggcgaagaa gatgaaggag aggttctcaa
     3841 cgacccttcg atggatctct tcgccgctac ggcagaagag ttgtattacg aaacggaatt
     3901 cgctctctac tgggaagacc agcaacggaa tcaggtcgcc aaattccagc ggatgattga
     3961 ggtagcaact gtgatcgctc agatcaccta cctctatgaa taccggccag aagcggaagg
     4021 gaaaatgcac cagtggccca ctcaggtata ctgcttcgtt ccacccgacc cgccaatcct
     4081 caactgatcc atcggatcaa gaacgccctg ccattcgtgg cagggcttct gtgtataaca
     4141 cacctttatc ttgtccgaat tcccgattga gtatataata cgctgtatca gctcgtaaca
     4201 ggaaagccgt atgcctgaag atgtttgcat cgtaaccgta ctggtagatc ttccgttcga
     4261 ggaagccgaa tgtctcgcga accgttatct cccccctatc gcctgtatgt ggaagtggag
     4321 atctaacggc cagaagctcc ctcagctcgt tatctccgct ccactgttgg gctacctcct
     4381 ttcgtcccgg atcgccagaa ggattgtcga gcagttaccg caagagaaga tcctcgagat
     4441 tttctttata cgcgaggtaa cccaggaaga aatgagaagc ggcgcgatag ctcagaacgt
     4501 gtacgggaga cgggaagtga tcgttgggtc actgggaaga agcaagctga ccccacgaga
     4561 taccgagtgg ggacacctct acaccggacg gggtatgccc cttccctttg gaagacgaaa
     4621 cgaagaagta gatgttatcg aagtacgcgc tccctcactt tcgatgatcg accagcttat
     4681 tcaaaagtgg cgagacggcg gcatcctctc ggaagatccg taactcatca atcactcgca
     4741 accataatgg ccaggatctt tcgggatcct ggccacacgc ataccctcac agggtaatta
     4801 acgaagtgga ggcaatgcct cacgcggcac ttcccacacg gtgtcagaga gaacttccac
     4861 cgtgttctca cgtaccgtca gcactggaac gccccacttt gacttcttga acttaagagc
     4921 ccatttcctt ttctgggcgt acaccaccga tccgagaaag tgcctgcctg caagaagaaa
     4981 cggcaaccag cactgctcgg ccgggaacat ggtatcgtac ggtacttttt ccgggtcata
     5041 ccaagtcggg cactcacttt catcggtctc gattggctca ccttcccagt tatgcgccac
     5101 aaacatataa ctgcgaacta ccttgacccc ttttaccccc acatcagcac cttgcgtagg
     5161 ccaacagaag tccataactc ctaggtagac gagatccatt tcctggatca aaacaccacc
     5221 ttcttcggcg gcttcacggc aggccgtccg gcggatcgtt tcgttcgggt ccttctttcc
     5281 gccgtatccg ttccaaagcc ccttgttcat gcgtttctta cctcgagcca gaagaactcg
     5341 attgttacgc atcaagaaga cgacagtaca ctccacacac tcttgaagca taacgctcct
     5401 aactccaagt cgagaaaaaa actctggccc cactataacc gcttcaccgc ataaaacaag
     5461 agaccggaga tccggtctct gttttttatt tgcttggtgc tggattgcgt tcaatcacgg
     5521 cgtcgataat gccgtattct ttggccgcag gagcactgag ccagaagtca cgctctgtgt
     5581 ctgcagttac cttttctaag gactgaccgg tattggccga caactcttca tttaagagct
     5641 gttttaagcg cagaatttcc cgggcagtaa tatcaatatc tgtggcttgc gcatgctcgc
     5701 ccacacttcc cattacctgg tgaatcataa ttcgggagtt cgggagggag taacgtttgc
     5761 ctttggttcc acccgccaag aggaacgctc ccatcgatgc cgccataccg atacagatgg
     5821 ttgagacatc tggtttaatg tagcgcattg tatcgtacat cgctaagcct gcgctgacgg
     5881 atccccccgg gctattaata aagatcgtga catctttttt gggatcttct gattctaaaa
     5941 agagtagctg cgccgtgaca aggttggcaa cgtgatcatc gataggagtg ccaataaaaa
     6001 taatccggtc tttgagcaaa cgggagtaaa tgtcgtacgc ccgttcccca aactgtgttt
     6061 tttctaatac cgtaggaaca agaatttggt tatgtgcatc catataactc cttatgtgga
     6121 cttaatataa cgaatatcca aaaaaatgta aagaacactt tacataaaag aaacgggacg
     6181 ccacaacggg cgtcccagac atcacgatta tggcggccag tgcatgcgct acttgctgcc
     6241 gttgcactcg tcgctcatga tctgccacgt ttgtaaccgc atgacaggta gcagcagaag
     6301 accaacgagc atagacacga acaacgcaca aacaattccc acaactaacc atatcttgcc
     6361 caacatggtg tcccccttgt cggtcgagca acctgctgtt agtatacgcc ttttattttt
     6421 tcttgctagc cgttttggcc gttgcgatct ctaccaattt ctcgatcgcg acatagccgg
     6481 cgtttttgtc tttttcgaga ccttcgcgct ccactacttc accgagacct aagccgatct
     6541 tgacggcttt ttcggctgat tcccgcatct ccttacgaat ttcttcttcc gttttcttca
     6601 tgtgctgaag atattgttca aagttaagac caaattgagc cgcttgctgc ttcaaagctt
     6661 caacctgacg gtctatctca cgctcaacaa ggctctgggg cacatctact tccaccagct
     6721 ttaaaaactc ttcaagagta gccgcttcgt cggcttgttt ggcctcttca ccaaagctgt
     6781 actcgcgttc ttggcgaatt gctgctttga gagtatcgag actctcttgt tcaaattctt
     6841 ttgcaaaatc atcgttgatc tcaggaagtt tacgttcgtg aacttcgtga acagtaactt
     6901 caaagacaac tttttctcca gccatatcct tggcatggta gtctttcggg aaagtgattt
     6961 caaatgaaaa agcatcgccg gcttttttac caattaactg gtcttcaaag ccagggatca
     7021 tggttttgct gccaatgacg attggataat gtttgctcgc taaacgatct tcgggaatac
     7081 cgtttcgttt tccggcaaaa tcgatctcga cgcgatcgcc ctctttggcc gcacgctcta
     7141 ctgtgtcgta tgttgcacga cgttcggcaa gatcggtgag cacagaatca atttctgtat
     7201 ccgtgacttc agcaactttc gcaggcttga ttttgatctt tgtgtaatca ccaactttaa
     7261 catcaggggc atagtcagct tcggcggtaa attcgagggt cgaatcctct ttcaccgtac
     7321 cgtcatcatt gagttcgcat agtttttcta acgtgtacga aggagcttcg ataagagaaa
     7381 ttttctcttt acgaactact tcttccaaaa gttctggtaa agcgtggtca accagttcgc
     7441 ttaaaatgcg tcccttacct aattgagcca caatcattgg tttaggcgcc ttgcctggac
     7501 gaaatccttt tacggctacc tgagccgaaa ggtggtgcag ggcatgctca gccgcgtgag
     7561 ctaaatcagc ggctggtgcc ttaacaatga tcttcatacg ggaattttgg agtttttcga
     7621 tagttaattg cataggtagt attataggtt aaatctgctg cttaatcaat gaaggggaga
     7681 aaaacctccc cagttcgcct agtaacaaac gctatgcgcg taatgctgta attcctggca
     7741 attctctccc tgctaaaaac tctaaacttg ccccaccacc cgtggaaagg aacgtaaagt
     7801 agtgctgtaa attatggtta cggatagctc ccaccgtgtc cccaccactc gagacgcggg
     7861 tttccgcgcc actgcgacca atcgattcag ctaccccaat ggtgcctcga gagaattgtg
     7921 gctcttcact tttccccatt gccccattcc aaaagactgt gttggcctta tgaatgtacg
     7981 tatcaaaatg tttaactgtc tccggcccga tgtccaacat cagttcttcc ccagttaaag
     8041 cgtacatcgg ttttatttcg ccatgctgat taatgtagtc aaccggaaca acaaacggga
     8101 tgtcgtcttg cgccattcgg caaatacact cttgggcaaa ttttacttgc tcgggatcga
     8161 ctttatgtgc tcccgtcggt tgactcatcg ccgctaagta cgtaacggct atcagtccac
     8221 caatcactac cacatcaacc ttgtcgagca agtcagcaat aatgggcgac ttgtcactcg
     8281 ccttagcacc gccgaggata gccacgtaag gagcctcacc ataatctcgt aacggcgtaa
     8341 gggcctggag ctcttttgca agctgaaacc ctgccacatg cggtaataag ccgggtaacg
     8401 cagacaccga ggcgtgagct cgatgactca ccgcaaaggc gtcgttgcaa tagatttcac
     8461 caaattttgc taacaatttg gcgaagccgg tatcgttaga ctcttctcca ctatgaaaac
     8521 gtaagttctc gagcataagg attggtgttg caggcatagg tcccagcatt tcagtcacta
     8581 agctcaccgg ctgctttacc gctgcggcaa tatagtcggc aaggatttgg ttcgatagag
     8641 ctggatcaac ctttcccttc ggacggccac ggtgggctaa aagagtcaac tgcgcctttt
     8701 tatctataca gtataaaatg gtatccataa ctgaatctag tcgataggta tcggctaacg
     8761 taccgtcaat attcaccgga acatccccgt caaagcgcag aaggatgcgc ttgccgctga
     8821 tgtcgatatc tttcaaagaa gggactacaa ggggctctga gttactttcc ataatgagtc
     8881 ttctcctcca agttgctcgc ctcttttcgc ccaccgggca aacgccaacg gaataggcag
     8941 aattgagagg taatatttta aatcatccca ctgttttagc caggcgatac ttatacccgc
     9001 cgaaagccgg ctccctttta cgagcatggc ataatcagga gaaaactgaa taatggccgt
     9061 gggagtatgc gccaagtaag gagaaaccgt acggccagtc cgtttggatg ttatcgcacg
     9121 ggcgatatgg ttgccatgtg caatcgccga agccttccag ccgctgtcgt caactgcggc
     9181 actgtctccc cccacccata cccctggctc atcgagagct tccaagtgag cattaaccaa
     9241 gacacgccct ttggcatcgt ggcggagacc tttggtcttt gcggcaagca atgagggctg
     9301 acgcccgtgc gcccatacaa ctgttttaat aggaccatcc tctgactcgg tctctaatcg
     9361 gcgggcaccc gaaggcgtaa gcttggtctt ggtcacaatc gaaattttat gttgctttaa
     9421 ccagcgagca acaacatgcg atactgccgg tttaaacatt ggtaaaattc gcgaggattc
     9481 ttcggccagt gtgatatgac agccacgcga agaagctccc tgccgtgctg cctgactctc
     9541 aatatatgat tgaagaccga gagcaagttc gacacccgag agcccaccgc cggcgatgag
     9601 cacatgcaaa tgacgctcct tatccctgga gaaattcgct tgaatttgct cccgtaaacg
     9661 gataaggtgc tcaaagttat gtgcgctaag gctagactgc cctgctcctt tttgcggcaa
     9721 agggactgta tcacttccgg gcgttaatac gaggtctgca tacgagaatc ggcgatgttc
     9781 agtttgtact tgctttcgct caacatcgat tcgtacgact cgttcttgaa tgtgatgaat
     9841 gtgcgtcccg gccaaaatat cgcggaacgg aataacagag actcgaggtg acgccggatg
     9901 catgagatca aaaagacccg gtgtgtagac atgaactggc gaaggatcga tgagaaaaat
     9961 tggacagtca agccgatggc caagaaggcg atctaaatct aatgccgtcc gaaggccggc
    10021 aaagcccccg cctataataa caatcggctg tgtttgcatc agtctttgtt actcttttcc
    10081 taagcctgcc attgcaacaa cttggtctac caaacggtta gcatatcccc attcattatc
    10141 gtaccaagcc acaactttga ctaagttgcc accaacaact tgagttaaac taagatcaac
    10201 gattgctgag gcgggattgc caacgatgtc tgttaagaca atttggtctt cggtaacttc
    10261 aagaatacct ttgtattttg cttggcgacc tgcttcttgt aagacagcct tcacttcctc
    10321 tacgctggtg tcttttttgg tcacaaatgt aaagtcagct aacgacccta ccgcggttgg
    10381 tacacggacc gataaaccaa ggaatattcc ctgaagtgcc ggcaaagcct tggcagccgc
    10441 ttttgtcgca ccggtgctcg ttggcaccat attctgcgct gcagcccggc cttcacggta
    10501 gtccttattg ggggcatcct gaatggcttg gctcgcagta tagctatgga ttgttgtcat
    10561 catcgccttt tcgatcccga atttctcttc gatgacagcc gctacagggg taatacagtt
    10621 cgtcgtgcac gaagcatttg agacaagctc ttggtcgccc agagcatctt cgttgacgcc
    10681 acgaacgacg gttgtcaccg tgtcgtcttt aacaggagcc gaaagaataa cccgtttggc
    10741 tccagcagca atgtgcttcc gcataccaat ttcatcggta aaacgtcccg ttgattcaat
    10801 gacaacgtca acacccaggt ctttccaggg taactcttgg ggttctttga ccgaagtaac
    10861 cgagaaggta tgatcatcaa ctgagatttc acccgttact ggagcacggt tctctacaac
    10921 cacccctttt acgtcggcgg gcagaacacc ataattggaa tcgtatttta aaagatatgc
    10981 cagatttgcg gcgtcagtta gatcatttac cgccacgata ttcttatgga gaccacgctc
    11041 atagagaata cgaaaagcaa cacgtccaat acgtccaaat ccgttaatcg caatttttcc
    11101 catatttctc cttttatttt cgtcgggata gcattacata cactacgaca ctaagtacaa
    11161 aaagcagcaa ggccggacca atactatcta cgaatcgacg agcgagcgat tcctctgggt
    11221 ggagtagtaa cttataggca aaggaaccaa taaaccaccc aatcgcaacc cataatgact
    11281 gtttccatgc gtttacttgt accataatta aacctttcac cttccctcac tatacaatcg
    11341 aacagaaatc gctacaaatc tggctgaaac gttttatttg gagcgaggct ccaccaacga
    11401 gtaacccatc taattcacgg atcgatccaa gcgcaccagc gttatcttct gtcacgctgc
    11461 ctccgtaaat aattcgcaca tgtacaccat acttctcact cacccagttt ttcacgtgaa
    11521 caatcgcttt ggccacataa tcaggagaag ccggattacc agttccaatt gcccacagtg
    11581 gttcataggc aaaaatacac tgtttaattt cttcgggttt tagtccttct aaatcatggc
    11641 ggagatcaat cgtaatttga ggtggaaatg tatgttgcgt tgcttgtccc atttctccaa
    11701 agcaaagaat cggcgtaatt ccctggttta agagctgttt tatttgctca ctcaccactt
    11761 ttccgttctc gtgatgatac tgacggcgtt cagaatgtcc caccaaacta taggtcacga
    11821 gcccttttaa ctgctttgcg gcaatatctc cggtgtatgc cccctccggc cattgactca
    11881 ccgcttggct cacgagagaa aaattcggtg gcagcattcg aaccgagtct ttaattggca
    11941 tgagccagac atgagaggga gcaatcgcaa tttctaaacc atggcatttt tcagcagctt
    12001 taatactccc ctgcgctaat gccatggcct cgggcaaagt taaattcatt ttccagttgc
    12061 cgatcaccat tcgcttcata agcctccttt tactttgatt ttccgaaaaa cgaatcaagg
    12121 tttttctgaa taattctatt ttttctcgcg ccgggttccc agcttaaaga agaagaaacc
    12181 aatcaccaag atacctgctg cgtagacaag gtttagggcc gaatggggct cttctgagat
    12241 cgacatataa tcaaaaaagg taatccccgc tgacgtaagg aacaaaaaac ttgcaacgat
    12301 atagagcgct cgctcaactt ttcctagacg tgaagccata ccacttaata agcgcgagca
    12361 aagagcgccc gtttgtgcga ggctcctcca cacttaatac atgttcccgt ttctgcaggc
    12421 tgatcaaagg gaatacaacg gattgttgct ttggtttcgt ctttaatctg cttctcgcat
    12481 tcttcggaac cgcaccagtg ggcatataaa aacgcaccat gctcaatacg ctcttggaat
    12541 gtagcatagt cgtcgatcgt aaacgtccgc tcatctcgac gcaaacgcgc ctggtagtac
    12601 aggttctttt ggatatcttc caaaagttgc ttcactttgg ccgtgacatc agctaagccc
    12661 actgtttctt tatctttggt gtcacgacgg acaacaacaa cttgatcagc tgctaaatcg
    12721 cgtggcccaa gttcgaggcg gactggtaca ccttttttct cccactcgtt aaacttatgg
    12781 cctggtgtgt agttatcccg ggtatcgatt cgcaccgtaa ccccagcttg ttttaatttt
    12841 tcagccacat tattcgcggc agtaacaact tgttctaaat ctgattcacg accaattgga
    12901 ataatgacga cttgtgttgg cgcaatgtta ggaggcaaga ccaacccatc atcatcactg
    12961 tgtgtcatta taaggccacc gatcaaacgg gtacttacac cccaactggt cgtccaaaca
    13021 tgtttggtcg tcccgtcttt atccgaaaac gtaatatcga acgctttggc aaaattctgg
    13081 cctagcgcat gcgaagtccc tgcctgtaag gccttaccgt cctgcatcat agcttcgatc
    13141 gtgtaggttg attcagctcc ggcaaaccgt tcggcggctg atttttcgcc gtcaatgacg
    13201 ggcatggcca tgtagttctc agccagatcc gtgtacacat cgagcatctt cttttgttct
    13261 tcttgtgctt cctgctgggt ggcatgggct gtatgcccct cttgccataa aaattctgtt
    13321 gttcgtaaaa acaaacgagg ccgaagctcc caccgcacta cattggccca ctggttaatg
    13381 aggagaggaa gatcgcgcca ggactgcacc caacggctaa acgaggcgtt aataatcgtc
    13441 tcagaggttg gccggaccac taatggctct tcaagctttt cgccaccagc gtgcgtaact
    13501 accgccaact caggagcgaa cccttcgaca tgctgcgctt ctttctttaa ataactttca
    13561 ggaataagaa gcgggaaata agcattttca tgccccgttt ctttaatttt tgcatctaaa
    13621 actttttgaa tattttccca gagggcatag ccatacggct taataatcat cgagccctta
    13681 ataccagaat gctcagccaa atcggcctgg gcaataatat catgatacca ttcggaaaag
    13741 tcctcgtggc ggggtgttat acgacttgag ctctgtttta cagtattttc atccatatcc
    13801 gaattctacg tcaacccaag agaaaagtaa acgaacagag gaatagtatg atatactccg
    13861 gccatgaaag aagaaatcct cctcactgaa tacgaaattt tgcgtcgcga agttattgaa
    13921 cgagtaggcg cactccatcg tctgattgca ctcgcttcca tcctcgcttt tctctcttta
    13981 ctcggaacta cctttctatt ctcctttcac gccacgcaaa cacagctgtg gacctacctt
    14041 ctcttcttac cccctgtttt tgccggaatt acctttaact accaagccaa ccaaatgacg
    14101 ctcgaagcag tcgctatgtt tagcgacaaa attcgtaagc agctcggtgg aaatgcaatg
    14161 ggttgggatg gattttatgg acgctggaaa aaagctgtcc agctaacaag ctttttaaaa
    14221 accctcccac tgctcatgcc actcgccatc cctttttatg cgctctcgca ggaacagtta
    14281 ctcaaaagtg agccacagct ggtatggctc accatcttcg atatcgtctt agcaactctc
    14341 gttgttatca acttttatta caaactactg cgcaaagtcg cagcgtaact ctagataagt
    14401 gattttagat ggtcgaaaat cgagaaccgt aacaagtctt gatatgtcac aatcacaaat
    14461 agtaagccaa tcgcggccaa gccggccaag gctacccact gccgatactt ctcgggtact
    14521 ggtttacgac gcaatgcctc caggccaaca aacagcaggt gtcctccatc cagaggtaag
    14581 ataggtaaga tattaataac cgccagattc gtgctaatta gtgccataaa ttgcatgagt
    14641 ggaccgacac ctaagcgtcg gaccgtaccg gttaaagcac ccacaccaat aataccgctc
    14701 acatcttcgc tcacttcttg tttaacaacc aaccgttgaa caaacccaat gaatccaagg
    14761 aacgaacctt taatttccgc accaacttca cgaatcgcaa taaagggagc acgataccac
    14821 gccgcccgaa cgtgactgac ttcctgcggc gcaataccta atgcgccttc accaacaggt
    14881 ggattttctc gaggagtcgc atgaacttca acggtccggc catcacggat ataggttaga
    14941 gaaatttctt tccccgcctg tgactgaatc gttgaaacaa gactttgtac cgaggtaagc
    15001 gattcgccgt ttattgccgt aatggtatct ccaggccgta acccggctat ttgagcagga
    15061 cttccgacag ttacgtcggc cagtataatt acaggttgga tatccaggcc tggatgttct
    15121 gcaaaactcg gagataacgg gagagacccc acactatagg ttgcggtaag aagagcccaa
    15181 gccaggacta agttcataac aatacccgcc actaaaacgg cggcacgtac ccccaccggc
    15241 tgggcggcaa acgaatcttt cgtcttcttt gcctgactcg gatcttgatc ctccccttcc
    15301 aagcggacgt acccgcctaa tggtagtaag ttaatcgcgt attcagtgtc tccccgcttc
    15361 catgaaaata accgtggacg aaaaccaaac gcaaattcgt gtaccttaat acccgatcgc
    15421 ttcgccaccc aaaagtggcc aaattcatga acaaaaacca acaaccctaa gataaaaata
    15481 aacacgagaa atgtaatcag catacagcga ctatagcatg atctgacgac aagcttaaga
    15541 aaaagagacc aaacaatcgg gtgtctggtc tctcatcttt gtatatttat tgcctagagc
    15601 gttttaagtt ctcgctcttt cgcttcgact aagtcatcaa cttgtttatt aaaggtatca
    15661 attgccttat tcaggtcttc ttcatagcga tagcgatcgt cttcagtaag ctcgcctgac
    15721 ttaacctgtt tttgcacttc atcccaagca tctttacgaa cattccgcag tgcgatttta
    15781 cccgcttctg ccttctggct gatcgtttta accagctgat tacgtcgatc ttcggttaga
    15841 gcagggagaa ccaatcgaat ttgtgagcct tcattcaccg gagaaagacc aaggtcgcta
    15901 ttacgaatag ctaactcaat atcgccaatc gactggcggt cccatggttg aatcaaaatc
    15961 attgttggtt cgggcaccga aattgaggcc atatttttca gaggagttgt attgccatag
    16021 tactgtaccg gcaatgtatc aaccaacgaa gcattcgccc ttccggtacg gatcgtcgaa
    16081 aactcgtgat tgacatgctc catcacatcc tgcatttttt tcttcatatc tgagaggata
    16141 tcggtcatag gatcctttct atatatctac gttgactata acaaaacttt gctacgcttt
    16201 cgagggtaac ttaaaccaaa aacgaggcct gagcctcgtt tttgtaagga ggttttcctt
    16261 attcagcgcc gagttcgaat cgagcaaatc gacgaatttg aatgttttcg ccaattttgc
    16321 caaccatttg atcgaggagc tggcgaactg taactttttc atcgctgaag aatggttgag
    16381 acatcaactc atcaaggtca gctggattca tcgaggcaat gtgcaatgtg agatctttca
    16441 caaacgtttt aaagtcgtca ttacgagcca caaagtctgt ttcgcagtta acttcaacca
    16501 tagcaccgat cttacccaag tgggcatagg tatggattaa accttgtttg gtttcgcggt
    16561 cagctttttt cgcaactacc atagcgctct tttctttgag caggtctcgt gctttagcca
    16621 tgtcgccatc agcctgttca agagcttttt tgatatccat aataccaaag ccagtctcct
    16681 cacggagctg ctttagtgta tccatactaa aggttgccat tggtcgttcc tacctttttg
    16741 actgtggtta actctgaggt atcaaccgag agttcttcag gagcttcaaa tttgatgcct
    16801 tggccttcgc aaatggcttc gccaacacgt tggagaacat attttaagcc acgtggcgca
    16861 tcatcgttac aaggaatgaa catatcaacc ttggttgggt cgccattact gtcgatagtc
    16921 gcgatagtgg tgacaccgag cttattcgct tcttcgatgg caatctgttc tgacttacca
    16981 tcaatgatga aaatcatatc aggaagattg gtcatatctt taataccgtc aaatgaacgg
    17041 tgaagtttgg caatttcgtc gttaattcgc ttctgctctt tcttcttgag cttagcaaac
    17101 tcgggggcag cagccgtttt ttcgagatca agcatgtggt ccaaactctt acgaatagtc
    17161 gtaaagttag tcaacatccc accgggccaa cggttggtga catatggcat gtctacagac
    17221 ttagccagag cagtgactaa accacgagcc tgtttttttg ttgcaacgaa aaggactttc
    17281 ttattttgag ctgcagctgt cttgactgct tcaagggcag tttcgaggtg ctcaagggtc
    17341 ttctctaagt caaaaatgca aataccgtcg cggacggaat atacatacgg acgagtcatt
    17401 ggcgtcgaat gctctttttt gtgtccgtag tggagaccta cattataaag ctcttggatc
    17461 gtcattggat gcatactatt cctcttctcc ctcagctgga gttacttctt cgccagcagg
    17521 cgtcccgtga ggggcttctg cttctaaagt aacttcttct ttaacttcaa cttctttcac
    17581 atcttcaacc ttagcctcag tgcgaggttt agcctgagcc atgcgttcac ggaaacggtc
    17641 aacacgacca gccgtgtcga tcagtttctg cgtcccagta taaaatggat gacactcttt
    17701 gcagatttcc actttcaaag attccttcgt gctcccaata gtgtgggtag cgccgcaaga
    17761 acaagtaacg gtggcgttag gataataggt tgggtgaatt ccagcttttg gcatagttga
    17821 gaagtttcct tttatgcata caaaaaaaag acgcgtttat gaacagatta atgataccga
    17881 aaatacgtca gagtgtcaat gtccgagacc aagaaatgcg aaaaatccgc atactcataa
    17941 aaaggtattg acgaattatt ggtttaagta tataattcgc cttgcatagt aacgagtaca
    18001 cgtctttcac tactcgctac cgcacatcgc atcttgccct atgggcacta tcggagaaac
    18061 ccgtgaaccg atcactgttt cgtggagccc tcgcgctcct gatcaccgca gttaccttgg
    18121 tttccgccgc ccacgcgggc ggcccgcgcg aaacgctgct cctgccggcg aactaccagg
    18181 ttgccaacct caccgaggac ggcctgggcg tcggcgccga gcgcatcggc cacggtcgca
    18241 tgacctcgtc gaagccgagc ctgttctacc tggccggcaa gaaggccaaa agctggccgt
    18301 accggccaca ggggccgggg caggtctacg gcttcgccat cgcgcgggac gacgccggca
    18361 acgtgctggc atccgtctcc cgcgaccgtc ggggaaatga gtggacggtc gtgatcaaca
    18421 gttcggacgg gagcttccgc cccctgcctc cgaacttcat accggcaacc atgagcccga
    18481 gcggcgtcat cgtcggcacc ggaatcgact tcatccttga gaagatctac cccgtccgga
    18541 tcctccgcga cgggacggtc gagtactggg aaggagaaga cgcctgggcc gtagacgtta
    18601 ccctcaccgg cgacgttctg atccagctcc cgggcgagta cgacgaagac gggaaccttt
    18661 caaagaaccc cgtgatcgag tactggaagg ccgacggatc cgtccaaggc gtcggagaag
    18721 gttacgctac cgcctgctcg aacagcggca cctatgtcgt agccgaccag gacggtgacc
    18781 aggtgcgttt caactgctcc acctacgtgg actacagcgc cgcatcaacg ttcctggcac
    18841 ccctcgaacc gaacgggtcg caagtgaacg ggacctacgc cgccgacgtc aacgacactg
    18901 gcgaagtcgt cggctccagc aacggccggg cggcctactg gccgggaacc atctccggtg
    18961 gggtcatccc cctcggtggc cccgtgctcc gtgggattac cgaagccacc gagctcggcg
    19021 ccatgatcga tctcccccgc ggctacacag cccagggagg ataccggatc accgggtccg
    19081 gcgcgatgct cgtcgggatc acccgcggca ccggccgcaa ggccgagtac cggacgttgt
    19141 atgtccgacc cactccaatc gaccttcctc gcccgctgca gtaggatctg ctcgggtcgg
    19201 ggacacaccg cccggcactt cgtgtgccgg gcgattcttt tatttaataa ttgacaaaaa
    19261 gcatatataa gtatataatt ttcccacaac ggccaatctt ttcgagacag gccgtagaac
    19321 tttgtacgtt ccccaaaagg gaaccttatc gaggagaaga tcgtgcgcaa ccagctgttc
    19381 cacagagcgc tcatggctct catcacctta tccgtgtgtg ccgcaggatt cgtggcggcg
    19441 gcaccggagt cacgaatcgt gcagcgaaaa gtggtaaccc tcccgaagtc gctgatgctc
    19501 agcaaggtca acgaccaggg cgtaggagcg ggggcgagca atcccctacg acccacccca
    19561 gtcaccgcca aactgagtgg aacctctgcg aagatccatc gcctcacccc ccaaaatggg
    19621 ttggcctaca gcctcaataa tgccggcgta agcgtcggag tcgttctgaa cggagagaaa
    19681 gaggtgccgg tcgtcttccg cgccaacggc gcgaccgaac cgatttccac cgctgtcaca
    19741 gtgtggccca gccacatcaa tgactccggc atcatcgttg gagagatcga cggggcaaca
    19801 tacaacacgt cgatcgccgc ccgcgttatc gctggtaacg ttgagatcta tctcgacacc
    19861 cgaccaatgg agccaggata tcggacgcaa gggtcaacgg cgatcgacat cgcgtcggat
    19921 ggcaccacct tggtgcaact ctactcggtg cgcaacaagg taggaatcga ggttctgccc
    19981 cgccaggtca tcctctggca cacagacgga acctccactc caattgccga ggacgcctgg
    20041 ggcagcgcca tttctgataa cgggcgcttt gttgtcggga tccagcaggc ttcccccatc
    20101 cgttgggaga tatccggctc cgctgtcaca accacccatg tcaccgagcc atctggcgcc
    20161 acttacacct acgggatcga tatcaacaac accggtgcca tggttgggga ggccgagtat
    20221 acggccatct actggccgac ccctggcgag ccgatcgccg tcacccagat ggcccggttc
    20281 aaaaagtcgg tgcaagcgct atcggcaatc tccctgacga acaaaggagg catcctggtg
    20341 cagtgccgca agggcaccac ctacttccaa tcctacctgc ggccataacg acgatacgtc
    20401 aggccgcaga agatccgccc ggtactctcg cagtaccggg caattctttt tattaaagta
    20461 ttgactcata aaccattttt tagtatactt tccccagttg ctttgtacgc accctagagg
    20521 agaatccaga tcgtgataac cacgagaaac cgttggcttt tgttgatcgc cttggcatca
    20581 ctcgccctgg cgttacctgg ccagacggcc accgcgaagt acaccttcat gccgctgccg
    20641 ccgcttttca cggccaacga cctgagcgat cagcgcgaaa tcgttggctc caccagggac
    20701 ggcgcgattg ggagcgatga agagtatgcc acgagctacc tggccagtat cgccggcctg
    20761 atcgagctcg accaactcag cgaacccagc gtctaccgcg gcatctcccc ttctggcgac
    20821 cacatcgccg gctggagcaa gaataccttg ggtgtctggg taggccaggt gtggagctgg
    20881 gcaataccgt cgtactcgac ggggcccaac accttccccg tcgcggtgaa taacagcggc
    20941 cgcagcaccg gagtccaggt cacctgggga ccgaataacc agatcgcaac ccagaaggta
    21001 tttcgcagcc cggccacggg caccactctc gagatcctcg acgaatgggg cgatccggtc
    21061 ggtataagca ccggcggaat cgcgacgtat accctcaccg aagtcctggg gggacgcgtg
    21121 atcaatcggg tcaagaactg gctgccgaac ggcagccgga tcgtgatcga tgaaggagag
    21181 tgcttcggtg tcagctcgag tggcgcaacc atcgccgggc agaaactaac gcaggcaaca
    21241 ctctggactg ccagcggcgc catgaacctc aaggcgggta cgaacagtct cgccctcgac
    21301 gccaatgacg ccggtacggc tgtcggcata agccgaggtc gggcttacct gttcgaacgt
    21361 ggcaagcgcg tcgacctcaa cacgatggtt aagcttccca aagagtggaa gaagtggcgc
    21421 ctcgtccgtg ccaaagccat caacaaccgg ggagatatcc tggtaacggc ccagaacctg
    21481 acacccagcc agggcaagcc ggcctacgtg gacgtcgccc tcctcaaata accgtcgcga
    21541 tcccgcggcg tgaacggtcc tccccttacc gggaggaccg tttttcgtta tgaaactcct
    21601 ccgagctatg gtaaaatcta cctgatgatt aaccagaagc tcatttcaga attacgtgat
    21661 ctcgaaacca tgaaacaaac cggatctgat ccggaatttt taacgatggt cgaagaagag
    21721 atgaccaaaa tccgcgaacg gatctttgct gaagaccctg acaatacccg tccccttatt
    21781 ttagaagtcc gctccggtac gggtggtgaa gaagctgaac ttttcgcggc tgacttactc
    21841 cgcatgtatc ttcgttacgc cgaaatcaag aactggaaga ccgatatcgt tgaaatgaac
    21901 gagtcttcaa tcgggggggt caaactcgca gtggtaggga ttcgtggctt tggcgcgtac
    21961 cctcttttga aattagaagg tggcgtccat cgcgtacaac ggatccccaa aaccgagaaa
    22021 gctggccgaa ttcacacctc ggcggcatca gtcgtcgtaa tgcccgaagt cgaagaacgg
    22081 gaattcgaca ttcgacccga agatattcgg atcgacgtgt accgtgcagg cggtaaaggt
    22141 ggacaaggtg ttaacacgac tgactcagcc gtgcgtttaa cccatattcc aaccggtatg
    22201 atcgtaacct gccaggacga acgctcccag ctcaaaaacc gcgacaaagc catgaaagtt
    22261 cttcgcagcc gcttgaagca gctcgatgac gaacggcaag ctcaagcaaa aggagacctg
    22321 cgtcgaagta tgatcggttc aggcgaccgc tcagataaaa tccgaactta caatttccct
    22381 caagaccgag taactgacca tcggataaac caatcgtggc acagcatgag ccgtattatg
    22441 ggcggtgaga tcgatacgat tacccaaaca atgcgtacaa ccgaactaaa cgaaattctc
    22501 cacgcagaag atagtgcata atgacacttc acacatggct caaaaatgcc accactcggc
    22561 ttgaagccca aaacatctcg agtagtcgca ttgatagcga actcatcctg gctcacattc
    22621 ttgagaaaga ccgggcttac cttattgcac atgtcgacga cactctttct tcggccgaag
    22681 aagctcatgc gaccgaactt cttgaacgtc gtcttaaaaa cgaaccgatg gcgtttttgc
    22741 ttaactcgcg cgaattttat aatctcacgt ttaaaaccga ccgtcgcgca ttaattcccc
    22801 gaggggagag cgagcctctc gtcgaaacag cgattgagtg gcttcaaaat cgccccgaga
    22861 gccagatcgt cgccgaaatc ggcacaggga gcggtgcact cattattagt gttgccaagg
    22921 cagtccctac ccacacattc ctcgctaccg aaataagtcc cgaagcatta agcttagcca
    22981 aagaaaacgc cgaattacac caggcctcta taaacttttt tgaaggaaat ctaggagagc
    23041 cactcctaac agattatgcc ggctcgatac atcttctgat gacaaacctt ccctatatcg
    23101 cgactgactt gctgactaat ttagatccaa ccgtgaccta ctacgaaccc aatttagccc
    23161 tcgacggcgg aggcgacggg ttaaatctat atcgccaatt tcttcctcat gccaaacagc
    23221 tcctcacatc tggcggttta ctcctgtgtg agcacgaaca cgaccacagc gaaatgatgc
    23281 gtgaattagt gcgagaacag tttcctgacg ctgtaataaa aaccgtacaa gatagccttg
    23341 gccacgaccg tctactcgtg tgccaaacct agtgtttccg ctcagaaacg taattgagta
    23401 gttcctgcca ctgctctcga tatgtctcct ctaaaggagc ttcatttaaa gccgattggg
    23461 cttgtgccaa aagctcacga attcgagtga ggccatattc aatcgcacca gaatttttaa
    23521 ataacgtttg aatgtgagcc tcctccccat cagtgcatgg ctgaccaaag taacgtcgca
    23581 aggtagctat ctcctgggcc gttccatgat taaagatgta gtaagtaaaa agcgtatgtt
    23641 gacgctgcgc caggtccgat agcacagatt tcgttgattc cgtacttggc gttaccaaat
    23701 cgagtaagtc atcctgaatt tgaaaagcga gcccaagcga tgtgccgaac tgttcacaaa
    23761 attgatccag cgctttgctt tgggtggcca acgctctccc aattttcata gggccaacaa
    23821 acgtgtaacg ggctgtttta aggttcattt tacgttcgat catatcttta gacgcgacat
    23881 cgagcgtcgt actatcaacg tcaatcattt gtccaagtat aacctcactt accatttgag
    23941 aaaccagttc gttcacctcg tatacctgcg ccatatcttc tgtttctacc tggagaaaac
    24001 atgaatgagc ccacgaatga accaaatcgc ctatcaaaat tgcctgactc tcactcacgc
    24061 ggtgtatgtc accgggccgc tgctgtgcct tcaatgcctg ggctgtttga atatgaaatg
    24121 ttggcaccgt atggcggctc gtcccttgat ccatcacatc atcgtgaata agacaaaaca
    24181 catgaaaaag ctccatccca attaaaggac cgtaaggaca ctgttgcgtc ccccccgcag
    24241 agcgatacat cagatctatc aggtaagggc ggacgcgttt gccgcccgcc atcaccaagt
    24301 cctcaagact ctcgatctgt tcttttaaga agtcatcacg aatcagggcc tgactttcac
    24361 gaatttttgt tcttacatag tcacgcaagt gcggttcaaa acgattctta aaagactgaa
    24421 tcatgactat ttctgttctt cgaggagcac gtctattttc ttctcttcgc ccttccgaag
    24481 aacagtgagt gtaacttttt caccaacttt atacttctgc aacagactca cgaggctatt
    24541 attctcatta atttcttgac cattaatctg ggtaataata tcgttttccg ctaaccctgc
    24601 tttgtctgca ggactgccgg gcacgacagg taaatcagct gggttggtac cacgataaac
    24661 caaggcacca tgatcaacag gcagctgagc tgaacgagct atttctttgt tgagggaaag
    24721 ataacgaacc ccgagccatg gccgaacgat ctttcctgtc ttttgaaccg agtcgagggc
    24781 tgtttttact gagtcgatgt tgatggcaaa tccaataccc tcggcgccac cagcaatagc
    24841 cgtattaata ccaactacct gtccggctaa gttcactaga ggaccgcccg agttacctgg
    24901 gttaatcgcg gcatcggttt gaagaagact gtcgagaact tcatcgctgg cagtaatacg
    24961 acgatctttg gccgagacaa ccccaacggt gacagtattt tggaactcac caagtgcatt
    25021 gccaatcgca atcacccatt gtcctacttg taaggcgttt gagtcaccca actcaaccgg
    25081 ttttagccct ttggcgtcaa cttttaagac cgctaggtca ttaaaggggt ccgtcgagag
    25141 cacttttgcg gtatagctct ttccgtcaga cgttaagaca gtgtattcag tatcgccttc
    25201 gctcacaacg tgtttgttgg tgactatcaa accatcagac gttaagataa agcccgttcc
    25261 gccacccttc tcctgcacag tccggccact gaaaaaatct tgaatattac ggctcgtcgt
    25321 aatactcacc accgaaggac tcaccttctt cgccgcgtca atcacggccg aactttcttc
    25381 gaggaccact cgttcttttt tagtcgtagt cgaagtaatg ccaccgtttg gattcgaaag
    25441 tccgggaata ttaaggacgg aaagactcaa gagagttgcc gatccaccta aaacaccagc
    25501 caacaggccg caggccaaag ctaacgcaac tagtttctgt cgttcaaaat ttgtctgcat
    25561 ggtatctcct ttactcccct tttggcactt cccagaccag ttcatcaaat ttagaattgg
    25621 gaacgattgc tagtgtgtca tcttttttaa gtatccgaac ttttcgacta tcaagggaat
    25681 aaatttgtcc ttccaaatca ccaattttta cctgccgtcc gattttaaaa tcattgcttc
    25741 gggcaagata aactccggcc gaaatatcag ccacgacctt atttgcccct gttgccaccc
    25801 cgattgcaat caccgccaga gaacttgaaa tcgcgatagc aatttgattg aggccaagag
    25861 attgaaacac taacgaaaaa aggccaaccc acaataccac cgacaaaacc gaaagtagaa
    25921 tcccctgcat aggcttcgtg atcttagttc gacggagtga ggcggcaaca atagcttgga
    25981 ttaacttaag gaagcagtaa ctgacaaaga gagtaattaa cgccccgggc aagcgaacaa
    26041 tagcctcttt gacatgttct aaaagcggag cgacaacatc aatggtgaga aaaaagtcag
    26101 tcatataaga tactcatttc caaaatattt ttacaagcta tcactaagta ttccataaag
    26161 cagtcacaaa gttaatgaca gggctcctaa actcgagaaa tttgagttaa ttgaatagcg
    26221 agtgtcaaac ggtccaaagc ttcacgcaat gaaatgcgat ggagacaaaa tgcttctcct
    26281 actcctttta ttgccaggag actgttcggc acttcgtcgt tagcgacgac aataagaggg
    26341 atgcggcggg tgggatattt cagcctcaag gtccgtgcga cctgttctat gttcgattga
    26401 gagagacgag cgctgatcaa tacggcttga ggggtttctc tacgtagcca gccggaaaaa
    26461 cgtgctggag aggcgagaga ggccact
//
LOCUS       DASWYB010000012         5686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_165432, whole genome shotgun sequence.
ACCESSION   DASWYB010000012 DASWYB010000000
VERSION     DASWYB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165432"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VGE59_00775"
     CDS             <1..618
                     /locus_tag="VGE59_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074221.1"
                     /translation="VNPINTTVSPPVKPRNLTVTNDGTNLLSLRWDSSYGATEYSVFR
                     SPTNNLAGAEQVGTNVATSRATDTLSINDHSTYYYWVRAYKPNAIVGSEAEGPVSITL
                     NATLRTPRFYRVQELSGKKIRLEWETYLEMNGTRANDSWEIWRKEDGASEFTKVYEGM
                     SNTTSKWTDTLPKAKTRYYYKMRAYKTIRGERLYSDFSNETSAKS"
     gene            complement(677..1831)
                     /locus_tag="VGE59_00780"
     CDS             complement(677..1831)
                     /locus_tag="VGE59_00780"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEY1074222.1"
                     /translation="MELTPKQQTLERIKSAEKILLLTHTQPDGDALGSLLALSSALTK
                     LNKKVTAVVDDRFSPSFRFMPGVNDLQTELPLRGDFVISVDIANTGLQKLGYKRDEAN
                     HRVMIVVTPAHGQLQPENVTFEGSGYEVDLIITLDCNSIDRLGDFYNKHATLFYETPL
                     VNIDHHPDNEQFGAVNWVDVTATSTAEILVSLLEALGRDVQLIDADVATNLLTGITTD
                     TSSFKNQNTTPKSLTVAAQLVAAGARQQEIVHHLYKVKPIWTLKLWGKALANIQERPD
                     LRILWAKLSKQDFADAGADVAETKGLIDELLKTAQGVAFVFLLTEKEDGIHISFRSVE
                     KGIPVSEVAKALGGGGHDVAAGALIDDSLSAAEERVLREVTAFWERRRTA"
     gene            2066..2335
                     /gene="rpsT"
                     /locus_tag="VGE59_00785"
     CDS             2066..2335
                     /gene="rpsT"
                     /locus_tag="VGE59_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY1074223.1"
                     /translation="MPISASAKKSLRVGQRRALENKAWKMRVKNTLKKTTAETLSVAF
                     SVLDKAAKRHVIHANKADRLKARLAKRFSTAATETVAAPAKTAKK"
     gene            2365..2919
                     /gene="lepB"
                     /locus_tag="VGE59_00790"
     CDS             2365..2919
                     /gene="lepB"
                     /locus_tag="VGE59_00790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY1074224.1"
                     /translation="MKHNNLFYQAGCLFRLGSTTLVVIALLALVKHFVFDILPLSGVS
                     MYPTYHDRDTYILNKISYVTGQPHRGDVVVLRFPGDPERARYIKRLIGLPGEHLAIKD
                     GKVFINDELLSESYIADDMITEGFNYPDITLGTDQYYLVGDNRPLSSDSRTWGTASKS
                     DFIGKVFYIVWPFNRARPIPSPVY"
     gene            complement(2966..4495)
                     /locus_tag="VGE59_00795"
     CDS             complement(2966..4495)
                     /locus_tag="VGE59_00795"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY1074225.1"
                     /translation="MLPVLREIFKGTTFYYKQLNLFLLCLVTLTGIVGVRYYAESQTP
                     QASTDLPRALAPQTEGSQVSYDIVVHPNGRTVVNGRTVPGAITYSEGRGKFLLVLIPK
                     ADVYTRSVEAKVTLPFSVDSMDTVSPHIYAVHGVGNSRFVSNDRQTVTFYAQDIFEGA
                     ELSIGFTFPEGYLKLSPIGAIQSYASSLSLPFWINFGLFVIPTFTLLVLGFLILKKIK
                     AYKQVSNNQVYPLAPQALPPAVVGALYHGQIGKREIAATLFDLANRGFLIIHHGSDMN
                     VTFSKGGALYGQQANTLRPFEVLLLHQIFGQEGFASRSKSIEAGLESELFSSKIAMTF
                     VNIYDAVVAEGFFIQSPNKYYLKYKIVGMVLFFISLAALIYGSFNLPEPAYILFVWVG
                     AMIASLIIVNMTPGLPRRTDRGNQILKQWMGFRNYLTWKKPIGTSKSSEFFAYLPYAL
                     TFECEHAWIARWENEVLTLPKWFSTEHTPYTAKDYQESIISVVEYLSGHLVAARPPDV
                     A"
     gene            complement(4553..5545)
                     /gene="holA"
                     /locus_tag="VGE59_00800"
     CDS             complement(4553..5545)
                     /gene="holA"
                     /locus_tag="VGE59_00800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY1074226.1"
                     /translation="MDLTRQCYFIHGGDTLSSYRALKAIETRFLKNQDSLSDFIVYDL
                     EDVSLDTLKQSLLTTPFLVTHRMFVLKNVFSASKATQEGIVALLPTISDSTFVVIYES
                     KSPDKRLSLYSWLTKHVKNDEHAVPQGAELIRRIQQIAQNYGVTVAPSASQFLGLHYG
                     HNTWQLDLEIRKLACAGLSRGKQAITDVEIKELSLAQHEEPAFALMDALRDGNLPQAI
                     RLYRSLITHADPMLLAGTLASQIRTWAKIVACLQTGDTRMESIAQRSGVKPFVVKLSL
                     PLARTLSIVSLKRCYRSLIRFDREVKDGSTPGSLGILLLIVRLHDTLNKKAQLS"
BASE COUNT         1642 a         1377 c         1293 g         1374 t
ORIGIN      
        1 gtgaacccca tcaacactac ggttagtcca ccagtgaagc cacgaaatct caccgtcacc
       61 aacgatggca ccaacttact ctcactacgc tgggattcga gttatggcgc cacggagtat
      121 tctgttttcc gtagtccaac caataatctt gccggagcag aacaagtcgg cacgaacgta
      181 gcaacctcgc gagctacgga tacgctaagc attaacgacc actcaaccta ctactattgg
      241 gtgagagctt acaaacccaa tgccatcgtg gggtcagaag cggagggacc ggttagtatt
      301 acgcttaacg ctacgcttcg gacaccgcgt ttttaccgag ttcaagaatt aagcggaaaa
      361 aagattcgtc tagaatggga gacatacctt gaaatgaacg gcacgcgagc caacgacagc
      421 tgggaaatct ggcgcaaaga agatggagcg agcgaattta ctaaagtgta cgaaggcatg
      481 agtaacacaa catcgaaatg gactgacacc ttgcctaagg ctaaaacccg ttactactac
      541 aaaatgcgtg cctacaagac gattcgtggt gaacgccttt actctgactt ctcgaacgag
      601 accagcgcca aaagctaaca tatgcacacc atacaaaaga gcgaccccgt gagaggtcgc
      661 tctttttaga ctcttactat gccgtacggc gacgttccca gaaagcagtg acttctcgta
      721 gtacccgttc ttcggcggcc gagagagagt catcaatgag ggcgccagct gcaacgtcat
      781 ggccaccacc acccaaagct ttagcgactt cagagaccgg gatacctttc tcgactgatc
      841 gaaaactaat atggataccg tcttcttttt cggttaaaag gaaaacaaat gcaacgcctt
      901 gagccgtctt aagcaactca tcaatcaagc ctttcgtctc ggccacatcg gcgccggcat
      961 cggcaaagtc ttgtttagaa agctttgccc aaaggatacg caaatcaggc cgttcctgaa
     1021 tgttggcaag agccttaccc cagagcttaa gtgtccaaat tggctttacc ttatataagt
     1081 gatggacaat ttcttgctga cgggcaccag cggcgacaag ttgtgcggcg acagtaagag
     1141 actttggtgt cgtattttgg tttttgaagc ttgacgtgtc agtcgtaatt ccggtaagca
     1201 agtttgtggc aacatcagcg tcaataagct gaacatcgcg acctaacgcc tcgaggagag
     1261 aaactaaaat ttcagctgta gaagtggcag taacgtcaac ccagttaaca gcaccgaact
     1321 gttcgttatc tgggtggtga tcaatgttaa ccagcggagt ctcgtagaaa agggttgcgt
     1381 gcttattata aaagtcgcct aaccgatcaa tcgaattaca atcgagagta ataataaggt
     1441 caacttcgta gccgctacct tcaaacgtaa cattttcggg ctgaagttgg ccgtgagcag
     1501 gcgtgacaac aatcatcaca cggtgattgg cttcgtctcg tttatagcca agcttttgca
     1561 aacccgtgtt ggcaatatcc accgaaataa caaaatcgcc acggagcggt agttcggttt
     1621 gcaaatcgtt cacaccaggc ataaaacgga acgaaggaga gaaacgatcg tccacaacgg
     1681 ctgtgacttt tttatttaat tttgtcagtg cgctcgaaag agcgagcaaa ctacccaaag
     1741 catcgccatc cggctgggtg tgtgtaagta aaaggatttt ttcagcggac ttaatccgct
     1801 cgagtgtttg ctgtttgggg gtgagctcca tgggaagttc tcctcgtttc ttaagccttt
     1861 tacaccaggc ttctacaccc ctccaagtgt tgatgcgatc ataccacctt ttacgatgtc
     1921 cgtcaaagga ggacttaacg agtgaatggc aggatatccg tctttacaga acaagggaaa
     1981 tcagatacaa tagtggctgt aactacttgc caggaggcat ctcaaaaaga gattgcctat
     2041 ggttttaagt aaaaggaaga cttttatgcc aatcagcgca tcagcaaaga aatcactccg
     2101 tgtcggtcaa cgccgtgccc tcgaaaataa ggcttggaag atgcgtgtaa aaaacacgct
     2161 gaaaaagacc actgctgaaa ctctcagcgt cgcttttagc gttctcgaca aagcagccaa
     2221 acggcacgtg attcatgcca ataaagccga tcgtctcaaa gctcgcttag ccaagcgatt
     2281 tagcactgcc gctaccgaaa ccgtagcagc ccctgctaaa acagctaaga agtaagacga
     2341 ccgaacaatc ctgtaggagg gataatgaaa cacaacaacc tgttctatca agcgggttgt
     2401 ttgtttcgtt taggaagcac aacgctcgtt gtgattgcac ttttagcgtt agtgaaacat
     2461 tttgtcttcg acattttacc cttaagcggc gtgagcatgt atccaacata tcacgaccga
     2521 gacacctata ttttaaataa aattagctac gtcaccggac aaccccaccg tggggacgtt
     2581 gtggtgctcc gtttccccgg cgacccggaa cgggcacgat atattaaacg ccttattggc
     2641 ctaccaggcg agcatctcgc tattaaagat ggcaaagttt ttattaacga cgaactcctt
     2701 tcagaatctt atattgccga cgatatgatc accgaaggat ttaactaccc tgatattacc
     2761 ctcgggaccg atcaatacta cctcgttggc gataatcgtc ctctttccag cgactctcgc
     2821 acttggggga ccgccagtaa aagcgacttt ataggtaaag tcttctatat cgtctggccg
     2881 tttaatcggg cccggccaat accgtcgccc gtctactaaa aaagaaggag cgctcgagcg
     2941 ctccttcttt tttagtaggg agaacctatg ccacgtcggg agggcgagcc gcaacgagat
     3001 gacccgacag atattcaaca accgatataa tcgattcctg ataatcttta gctgtatagg
     3061 gagtatgttc cgtactaaac cacttgggca aggtgagaac ttcattttcc caacgcgcaa
     3121 tccaggcatg ttcgcactca aacgtgagcg catatggtaa gtatgcaaaa aactcactcg
     3181 acttcgatgt cccaattggc tttttccacg tcaggtagtt acggaatccc atccactgct
     3241 ttaaaatttg attacctcga tcagtgcgtc ggggcaaccc tggcgtcata ttcacaatga
     3301 tgagcgacgc aatcattgcc cccacccaca caaagagaat gtatgccggc tcgggcaagt
     3361 taaacgaacc atagatcaga gccgctaaag atataaagaa aagaaccata ccgacaattt
     3421 tatattttaa gtagtactta ttcggggact gaataaaaaa gccttcggca acgaccgcat
     3481 cgtagatgtt gacaaaggtc atcgcaattt tcgagctaaa caattcgctc tctagtcctg
     3541 cttcgattga ttttgaacgg ctggcaaacc cttcttgtcc aaagatttga tgaagcaaga
     3601 gaacctcaaa gggacgaagc gtattagctt gctgaccata gagggcgccc cctttactaa
     3661 aggtaacgtt catgtcggag ccatgatgga taattaaaaa gcctcgattc gccagatcaa
     3721 aaagggtggc ggcaatttct cgcttgccaa tttgaccgtg ataaagtgct cctaccactg
     3781 caggaggaag cgcctgggga gctaatgggt atacttgatt attagagact tgtttatagg
     3841 cttttatttt tttaaggatt aaaaacccta ataccagcaa tgtaaaagta ggaataacga
     3901 agagaccaaa gttaatccag aacggcagcg agagcgacga ggcatacgat tggatagccc
     3961 caatcggact taacttaagg tagccttctg ggaaggtgaa accaatcgaa agttccgccc
     4021 cttcaaagat gtcctgggcg taaaaagtca ccgtctggcg atcattggaa acgaagcggg
     4081 aattacccac cccatgaacc gcgtaaatat gaggagacac agtatccata ctatcgaccg
     4141 aaaaaggcaa agttacttta gcttctacag atcgggtgta cacatcggct ttaggaatga
     4201 ggactaataa aaattttccc cggccttcgg aatacgtaat cgcacccgga accgttcgac
     4261 cattgacgac cgtccgaccg ttagggtgga caacaatatc gtaagaaact tgactcccct
     4321 cggtttgagg cgcgagagcc cgaggcagat cagtcgaagc ttgtggcgtt tgactttcag
     4381 catagtagcg cacgcctact atccctgtaa gcgtgactaa acacagcaaa aaaagattaa
     4441 gctgcttgta ataaaatgtc gtccctttaa aaatttcccg tagtacgggc agcacgtatc
     4501 cctccttcat aagaccggag cggttcacat cgaatcgtgt gagcgaagta tgttaagaca
     4561 gttgagcctt cttatttaag gtatcatgca ggcgcacgat cagcaacaaa attccaaggg
     4621 atcctggcgt tgatccatct ttaacttctc ggtcaaaacg gatcaacgaa cggtaacaac
     4681 gctttaacga cacaatcgaa agagtgcgcg caagcggcaa actcagtttg acgacaaatg
     4741 gcttaacccc cgaacgttga gcaatagatt ccatacgcgt atctcctgtt tgtaaacaag
     4801 caacaatttt tgcccaggta cgaatctgac ttgccaatgt accggctagc agcatcggat
     4861 cagcgtgagt gattaatgat cggtagagac ggattgcttg cggaagattt ccgtcccgca
     4921 gcgcatccat taatgcaaac gccggctctt cgtgttgcgc tagagaaagc tcttttattt
     4981 cgacgtcggt aattgcttgt tttccacgag agagaccggc gcatgcgagt ttgcgaatct
     5041 ctaaatccaa ttgccatgta ttatggccat aatgtagccc caaaaattgg ctcgctgaag
     5101 gggcaaccgt cacgccataa ttctgagcga tttgctgaat tcgccgaatg agttcagccc
     5161 cttgcggaac agcatgttca tcatttttaa catgcttggt aagccacgaa taaagtgaga
     5221 ggcgtttatc aggggatttt gattcatata tcaccacaaa cgttgaatcg gaaatcgttg
     5281 gcaagagtgc gacaatccct tcctgcgttg cctttgacgc tgaaaaaaca ttttttagga
     5341 caaacattcg atgcgtaacc aaaaaaggag tggtgaggag agactgtttg agcgtatcaa
     5401 gcgatacgtc ttcaaggtca tacacaatga aatcactcaa gctatcttgg ttcttcaaaa
     5461 aacgggtttc gatcgccttg agcgcccgat agctacttag cgtatctccc ccatgaataa
     5521 aataacactg ccgagtaaga tccatgcttc ctttatagca catgtctcaa acgaagctcc
     5581 acgtacgctc attcctcctt ctcatcgtag agaggaatga gtacaatgaa cccaccatga
     5641 aacctcgtga accgctcact tatcctcaag cacttcagta tgcact
//
LOCUS       DASWYB010000013         8354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_216300, whole genome shotgun sequence.
ACCESSION   DASWYB010000013 DASWYB010000000
VERSION     DASWYB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216300"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1370)
                     /locus_tag="VGE59_00805"
     CDS             complement(51..1370)
                     /locus_tag="VGE59_00805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY1074227.1"
                     /translation="MKIAIIGTGYVGLVTGACLAESGHMITCIDKDMAKIERLKNGEI
                     PIYEPELDDLVKRNSEKDRLLFSTDLAKTLPDVDVVFLAVGTPPKKDHSADLSAVQAV
                     SKEIGQNLTRHILVVTKSTVPVGTSELVRSTIQKHLKQPIEFDVASNPEFLREGAAVK
                     DFMEPDRIVVGIDSPKSEALLREIYRPFIQKDISFITTTIKSAEVIKYASNAFLATKI
                     SFINEIANFCEASGAKIDDVAMGMGLDKRIGARYLHAGLGYGGSCFPKDVKALVASGE
                     KYGVPFKILHAVEAVNQKQRLRIVEKLEKHLGSLASKRIAVWGLSFKAKTDDIRESPA
                     LTIIDALLKKGATVCAYDPVVKEVPQKISLSPFMYDCCNDADALVITTEWEDFLYPDF
                     REIKKRLRTPLIIDGRNLFDPDTLDPMGITYDSIGRPLPPHNELLSS"
     gene            1473..2195
                     /locus_tag="VGE59_00810"
     CDS             1473..2195
                     /locus_tag="VGE59_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY1074228.1"
                     /translation="MKGIILAGGRATRLRPLTKITSKQLLPIYDKPMIFYPIETLVRA
                     GIKDILIIISPDYAGHFLNLLGSGKDFGAKFSYEIQDSPGGLPEAFVIGENFIGKDNV
                     TLILGDNIFDYDFSDAIRSFDKGGRIFAKEVHDPERFGVVAFDEHNKVYSIEEKPKVP
                     KSNYAIVGLYVYDNRVIEYSKNLAPSARGELEITDLHRKYLKDGELDVRLIDGIWEDA
                     GTFDSLLRANNIMAEKAKKGAA"
     gene            2192..2731
                     /locus_tag="VGE59_00815"
     CDS             2192..2731
                     /locus_tag="VGE59_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY1074229.1"
                     /translation="MTPDLLTADSREVYTIQSYEKAPQIEGVVAKDIACFVDDGGYFM
                     ELGRFDKGISQLFPDFEAKQMSYSQVLPGAVKAFHLHMNQEDVWFIPPHERLLVVLSD
                     QRENSPTKGVTMRFVMGGGKSRLLYIPRGVAHGAANLGDAPASIIYFVNQQFNLEKPD
                     EGRLPWDFLGADIWEMTKG"
     gene            2742..3728
                     /locus_tag="VGE59_00820"
     CDS             2742..3728
                     /locus_tag="VGE59_00820"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY1074230.1"
                     /translation="MRILVTGGAGFIGTNFVYHMVSLGHDVTVLDKLTYAGGRDNLEP
                     LGSKVKLVVGDICDSACVGTAVQGIDWVAHFAAESHNTRSEFDPDVFYRTNVEGTRVM
                     LDESLRANVKRFLHISTDEVYGSIENGYFAEADKIKGDSQATSAYSKSKSLADDLALE
                     YAAKGAPILVSRTTNNFGPWQFPEKALPRWITNLLMGKKIPLWGEGLQVRDWLYAPIH
                     AEALNFVLEHGQLGEAYNIAANHDPEITNKQAALWLCEILHKDPEEMIEYIPDPRPNH
                     DFRYALNTEKIASLGWKPSGKARSQFEETVEWYQSHEAWWRKRKEEAERIYQ"
     gene            3739..4590
                     /gene="rfbD"
                     /locus_tag="VGE59_00825"
     CDS             3739..4590
                     /gene="rfbD"
                     /locus_tag="VGE59_00825"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY1074231.1"
                     /translation="MRIFLTGAQGQLGKACQAEFATHKLFLADIDTCDITDSESCRTA
                     LESFSPDLIIHTAAYTNVKQAEVDPDVCYRVNLDGTYNLARYARAHSCRFVYISTDFV
                     FGGESDKPYNEHDAVNPQSVYARSKLAGELLVQDLVRDHYILRTAWLYGAGKNFVQTI
                     ITLAQTKEELPVVSDQYGTPTYAVDLAHYISQVLQTQTPGIYHATNEGSASWADFARE
                     ILRLKGLTNQVIPITTQEAAIRFSDPTVRPAYSVLNKDKLKRITPVRKWQEALQDYLS
                     APTTRNK"
     gene            complement(4557..6107)
                     /locus_tag="VGE59_00830"
     CDS             complement(4557..6107)
                     /locus_tag="VGE59_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074232.1"
                     /translation="METRRGHYFLFFTVLLGLVFFSYLNQLIFRYSVPPGLDAVPHNE
                     VVRNILNGDIGQIFRYHTLWHILTILLSSVTRLPTITAMAWLGPLLLVTSSISLYIFN
                     RRFFSPLAGYTSLLLLGFTSLQPLQTLYDGSFPNVLAAGTVLPLAFILFENIFHSVKK
                     ARAIFLFVLGFIILAFSHHLTTLYALATMGIFLLVFLTQYCRQKRISWFLILFVLIVS
                     LLLGFLTLNWLLHSPLTENSVFSLANQFMATSFHYPFIHFKGVLDDPNAIWRISLYPQ
                     EIGEAVVYLGIMGFFAGLYFLKKRPADTAWNAVALLIIWSCVLFLGSRMEALAYPVRL
                     ARDLAIPLALLSGVFVHLVWSYIVEHGLPRWLFAVIIILIIFLAYPSTTKRFAIATHP
                     SNLVHHLPVDQLASDYLEKNTPEGSKILIFHDNYYLGFFLPNREVMWQLPTAMIPKVL
                     HLVDLKETLKGIDYVYVEERPDRESSQVNNTDITYNYLRSPVVKLVATFEQPEKKIYL
                     FRVVGADK"
     gene            complement(6148..7521)
                     /locus_tag="VGE59_00835"
     CDS             complement(6148..7521)
                     /locus_tag="VGE59_00835"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY1074233.1"
                     /translation="MTSFQRFSLPVVAACALGALLFLFVPQDPRIAFAAAAGGIVLLG
                     LFAAFRSIRLFPLIIMVSLIIGQLVRLSISSGSSGALLLTDIATGAYVTIGVIHMLLT
                     KKSPPRTLSVVALIGVVGWMFISLLFGSRDLAGTEIIIALFYAIRFTLMIGAIIVTAS
                     FYPQKANLAQLLNWLIVTGIILVLLGFAQITFLPDFRFMARYGWDPHQGRMLSTYFDP
                     NFFGMFLVIIFSLLLSRFFFFPKAQLTIVALLLFIAAAILLTYSRSSYLALLISLGII
                     LTIRSWKLVLIMGIALTVVSLSIPRVKERIIGAITVDETAVDRIESWQETFVIIRSSP
                     WIGVGYNAYGPAQVKYGLKNNLLGRSSQGSDSSALLILATMGVIGLAFFTFLFGSFLY
                     EAVILYRHTKDPFWQSLGLAILGILPALLIHSQFVNSLFYPLILVPLTFLLATLIQGV
                     RRLGTQA"
     gene            7669..>8354
                     /locus_tag="VGE59_00840"
     CDS             7669..>8354
                     /locus_tag="VGE59_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074234.1"
                     /translation="MSIRTHLITLQKWWLFALLFLIPLSLQYLIKTPVAISTLNILSL
                     GFIFLGVLNALTGGVPIRPVQHRSYVWVILLLLGALAYALLFTHPLRNAVGLWTSRLS
                     QPLLVGYFAYQLLANRVITLSEPIRALFYSLVPLLILGGMQLTGFLPYADTDRVTASY
                     FFPNTFARYVGIVLLISLPWILFSIRQYKRLHLGLWILGVLLLLSSKSYGGTVAFFGG
                     LTALLFALPNP"
BASE COUNT         2342 a         1738 c         2019 g         2255 t
ORIGIN      
        1 gtcttttttc tccttagccg ataataacag acaagcagat aggccgcaat ttagcttgaa
       61 agtaactcgt tatgtggggg gagtggtcgg ccgatgctat cataagttat acccatggga
      121 tcaagcgtat ccgggtcaaa aagattacga ccgtcgataa taaggggagt gcggagccgt
      181 ttttttattt ctctaaagtc ggggtagaga aaatcctccc attcagtcgt aatcactaat
      241 gcgtctgcat cattacagca atcgtacatg aaggggctca gagagatttt ttgggggact
      301 tcttttacca cggggtcgta ggcacaaacg gttgcccctt tttttaacaa ggcatcaata
      361 atggttagag cgggagattc tcggatgtca tcggttttgg ccttaaaaga caatccccat
      421 acggcgattc ttttactagc aagtgacccg agatgctttt ctaatttctc gacaattctc
      481 aaccgctgtt tctgattcac tgcttcaacg gcatgaagta ttttgaaagg tacaccatac
      541 ttttctccgc ttgcgactag ggctttaaca tctttgggaa aacagcttcc gccgtatcct
      601 agtcccgcat gaaggtatcg ggcgccgatg cgtttatcta accccatgcc cattgccacg
      661 tcgtcaattt tggctcccga tgcttcgcaa aagttggcga tttcgttgat aaaagagatt
      721 tttgtagcca agaaggcatt cgaagcgtat ttaattactt cggcactttt aattgtggtt
      781 gtgataaaag aaatgtcttt ttggataaaa ggtcggtaaa tctctcgcag taaagcttct
      841 gatttcggac tatcaatacc tacgacaatc cggtctggct ccataaagtc cttgactgca
      901 gctccttccc gtaaaaattc agggttagaa gcaacgtcaa actcaattgg ctgcttgagg
      961 tgtttctgaa tagttgatct tacaagctca gaagtgccca ctggaacggt cgatttggtt
     1021 accacgagga tatgacgagt gagattctga ccgatttctt tgctcacagc ttgaacagca
     1081 gataggtcgg cgctatgatc ttttttcggt ggagtgccga cagcaagaaa aacaacatcc
     1141 acatccggca acgtcttcgc gaggtcagtg gaaaagagga gcctgtcttt ttcagagttt
     1201 cgcttgacta aatcatcgag ttcgggctcg tagatgggga tctctccgtt tttcaagcgt
     1261 tctatctttg ccatgtcttt atcgatacac gtaatcatat ggccagattc tgccaaacac
     1321 gcacccgtaa caagtcctac atagccagta ccaataattg caattttcat acctgcatac
     1381 taacgtgcgg tatcatttcg agcaagggaa ttgtaagggg atgttttgca aacagtgtca
     1441 ggcatgttat tttagacagg taaaggagcc ctatgaaagg tattattctt gctggtggcc
     1501 gagcgaccag actgcgaccg ttaacgaaaa ttacgtcgaa gcagctctta ccgatttatg
     1561 ataaaccgat gattttttat ccgattgaaa cgctcgttcg agcggggata aaagatattc
     1621 tcattatcat ttcacctgac tatgcaggac actttcttaa tttgcttgga tcgggaaaag
     1681 actttggggc aaagtttagt tatgaaattc aagatagccc aggtggcctc ccagaagcct
     1741 ttgtgattgg ggagaacttt attgggaaag acaatgtcac acttattttg ggtgataaca
     1801 ttttcgatta tgatttctcg gatgccattc gttcatttga taaaggcggc cgaatttttg
     1861 ccaaagaagt tcatgatccc gaacgcttcg gtgttgttgc ctttgatgag cataataaag
     1921 tgtattcaat cgaagagaag ccgaaagtgc caaagagcaa ttacgccatt gtggggttat
     1981 acgtctatga taaccgggtt attgagtatt ccaaaaatct cgctccttcg gctcggggag
     2041 agcttgaaat taccgacttg cacagaaagt atctcaagga cggagagtta gatgtacggc
     2101 ttattgacgg aatttgggaa gatgcaggaa cgttcgacag tctcttacgg gctaataata
     2161 ttatggcgga aaaagctaaa aagggagcgg catgacacca gacttgttaa cggctgattc
     2221 tcgagaagtg tacaccattc aatcctacga aaaggctcct caaattgaag gagtcgtggc
     2281 caaagacatc gcttgttttg ttgatgatgg cggatatttt atggagttag ggaggtttga
     2341 taaaggcatc agccagctat ttcccgattt tgaagccaag caaatgagct actcgcaagt
     2401 tttgccgggc gcagtcaaag cgtttcacct tcatatgaat caagaagatg tttggtttat
     2461 tcctcctcat gaacggctgt tagtggttct ctctgatcag cgagaaaatt ctccgaccaa
     2521 gggggttacc atgcgttttg ttatgggggg agggaagtcc cgattactct atatccctcg
     2581 tggtgtggct catggggccg ccaatctcgg cgatgcgccc gcttctatta tttattttgt
     2641 caatcagcag tttaacttag aaaaaccaga tgaaggccgg ctgccatggg attttctcgg
     2701 cgccgatata tgggaaatga ccaaagggta gaaggagttc tatgcgaata cttgtaacgg
     2761 gcggtgctgg ctttattgga acgaacttcg tctaccatat ggtttccctg gggcatgatg
     2821 ttactgtcct tgataaactc acgtatgcgg gtggtcgtga taatttagaa ccactcggtt
     2881 caaaagtaaa gctagtggtg ggcgatattt gtgactcggc atgcgttggt acggcggtcc
     2941 aaggaattga ttgggtcgct cattttgcag ctgaaagtca taataccaga agtgagtttg
     3001 acccggatgt attttaccgt acgaatgttg aaggtacccg agttatgctt gatgaatcgt
     3061 tacgagccaa tgttaaacga ttcttacata tctctactga tgaggtatat ggatcaattg
     3121 aaaacggata ctttgcggaa gctgacaaga taaagggtga cagccaagct acgtcggcat
     3181 attcaaagtc aaagtcactt gctgatgatc tcgccttaga gtatgccgct aaaggtgccc
     3241 ccattttagt gagccgaacg acgaataatt ttggtccgtg gcagttcccc gaaaaagcct
     3301 tgccacgttg gattaccaat cttcttatgg gaaagaaaat tccactatgg ggtgaaggtt
     3361 tacaggttcg cgattggttg tacgctccta ttcacgctga ggctctcaat tttgttttag
     3421 agcatggcca actcggagag gcgtataata tagccgccaa tcatgacccc gaaattacca
     3481 ataaacaggc ggcgttatgg ttatgtgaga tacttcacaa agatccagag gagatgattg
     3541 aatatatccc tgatcctcgg cccaatcatg attttcgtta cgcccttaat actgaaaaga
     3601 tagcgtcctt aggctggaag ccttcgggta aggcccggtc tcagtttgaa gaaactgtcg
     3661 aatggtatca gtctcacgaa gcgtggtgga gaaagcgtaa agaagaagcc gaacgcattt
     3721 atcaataagg aggtgggagt gaggatcttt cttaccgggg cacagggaca attaggaaag
     3781 gcctgccagg cggagtttgc cacgcataag cttttcttgg ctgatataga tacatgcgat
     3841 attacggatt cggaatcgtg tcgtacggct ctggaatcgt tctcgcccga cttaattatc
     3901 cataccgcag cgtatacaaa tgtaaaacag gccgaagtag atccagatgt ctgctaccgg
     3961 gttaacctag acggaaccta taatcttgct cgatatgccc gggcgcatag ctgcagattt
     4021 gtatacatat caacagattt tgtatttggg ggagagtcag ataaacccta taacgagcat
     4081 gatgcagtaa atccacaaag tgtatatgcc cgttccaagc ttgcgggaga actacttgtg
     4141 caagacttgg ttcgtgatca ctatattctt cgcacggcat ggttatatgg cgcaggtaaa
     4201 aactttgttc agacaattat tacattagcc caaacaaagg aagagttgcc agtcgtctct
     4261 gatcagtatg gtacgccaac ctacgctgtg gatttagccc attatatcag ccaggtttta
     4321 cagactcaga caccgggaat ttatcatgcg actaacgaag gcagcgcatc ctgggctgat
     4381 tttgctcgcg aaattcttcg gctcaaaggg ttaacaaatc aggtaattcc tattactacc
     4441 caagaagcgg caattcgttt ttccgatccg accgtacgtc ccgcttactc tgtattgaat
     4501 aaagacaagc tcaaacgtat tacaccggta cggaagtggc aggaagcctt acaggactac
     4561 ttatccgccc ctacgacccg aaataaataa atcttctttt cgggttgctc aaatgtagca
     4621 actaatttta ccacgggtga gcgaagataa ttatatgtaa tatcagtatt attcacttgg
     4681 ctactttctc ggtcaggtct ctcttcaaca tagacataat caatcccttt aagggtttcc
     4741 tttaaatcta ccaagtgaag gactttagga atcatcgccg ttggtaactg ccacatcact
     4801 tctcggtttg ggaggaagaa tccgaggtaa taattatcgt ggaaaatcag aattttcgat
     4861 ccttcaggag tgtttttctc aagataatca gaggccagtt gatcaacagg taggtgatgg
     4921 accaagttac tagggtgagt tgcaattgca aaccgttttg tcgtgcttgg ataggcaagg
     4981 aaaataatca agataataat gactgcaaag agccagcgtg ggagtccatg ctcaacaata
     5041 tagctccata caagatggac aaaaacgcca ctcagcaagg ctaatgggat tgcaaggtcc
     5101 cgcgctaatc gaacgggata tgccagtgct tccatccggg atcccaggaa aagaacacag
     5161 ctccatataa taagaagagc gactgcattc caagctgtgt cggcgggcct ttttttgaga
     5221 aagtagaggc ctgcaaaaaa tcccattatt ccgaggtaga caaccgcttc tccgatttct
     5281 tgaggataaa ggctgatccg ccatattgcg ttgggatcgt caaggactcc tttgaagtga
     5341 ataaaggggt agtgaaagct tgttgccata aactgatttg cgagactaaa gacggagttt
     5401 tcagtgaggg ggctatgtaa cagccagttg agagtgagaa atccgagtag taaagatact
     5461 atcagtacga ataaaataag gaaccaactg attctttttt gacgacagta ttgtgtgaga
     5521 aaaacaagta agaatatgcc catagtagcg agtgcataca gggtcgtgag gtgatgagaa
     5581 aaagcaagaa taataaatcc aaggacaaag agaaaaattg ctcgtgcttt tttaacggag
     5641 tgaaatatgt tttcaaacaa aataaaggct aaagggagta ctgttccggc ggctaaaacg
     5701 ttagggaagc ttccgtcata aagggtctgg aggggctgga gagacgtgaa tcctaaaagt
     5761 aaaaggctcg tgtagccagc gagaggacta aagaagcgac ggttgaaaat atacaggcta
     5821 atggagcttg tcactaagag caatgggccc aaccaagcca tagctgtgat ggttggaagt
     5881 ctggttactg agctcaggag tatggtcaaa atatgccaga gggtatgata acgaaagatc
     5941 tggccaatat cgccgtttag gatgtttctt accacttcat tgtggggtac tgcatctaag
     6001 cctggtggca cgctgtagcg aaaaataagt tgatttaagt aactaaaaaa aacgaggccg
     6061 agcagcaccg tgaaaaacag aaaatagtgt cctctccttg tttccatatt tctatgctat
     6121 caggcaaacg cttacgatcc aagaggctta ggcttgtgtg cctagtcgtc gcactccctg
     6181 tatgagagtt gctaaaagaa atgtgagtgg gactaaaatg agtgggtaaa aaagactatt
     6241 aacaaattgg ctatggataa ggagggcggg taatatcccg agaatagcca atccgagact
     6301 ctgccaaaaa ggatcttttg tatgacgata caggataacg gcttcatata agaaactgcc
     6361 aaagaggaat gtaaaaaatg ctaaccctat tacgcccatt gtggcgagga tgagaagtgc
     6421 gctcgaatca ctgccctgtg atgatcgtcc aagtagattg ttttttagtc cgtacttaac
     6481 ctgtgctggc ccatacgcat tgtatcccac cccaatccaa gggctggagc ggataataac
     6541 gaatgtttct tgccacgatt cgattcggtc aacggctgtt tcatctacgg taatagcacc
     6601 aatgattcgc tctttcaccc gagggatact caaactgacg acggtgaggg ctatccccat
     6661 aataaggaca agtttccagg aacggattgt gagtatgatc cctaggctaa taagaagggc
     6721 gagatagcta ctgcgagaat aggtgagtaa tattgccgct gcgatgaaaa gaagtaatgc
     6781 cacaattgtc agttgggctt tcgggaaaaa gaaaaaacgc gaaagcagga gagaaaaaat
     6841 aatcactaaa aacatcccaa agaaatttgg atcaaagtag gtagaaagca tacggccttg
     6901 atgaggatcc caaccataac gagccataaa cctaaaatct ggaagaaaag taatttgagc
     6961 aaatcctagt aggactaaaa tgattccagt gacgataagc caattgagta actgggcaag
     7021 gtttgctttt tgagggtaga agctggctgt cacgataatc gctcctatca taagggtaaa
     7081 gcgaatggca taaaagagcg caattataat ttcggtgcca gcgagatcac gactaccaaa
     7141 gaggagggag atgaacatcc atcctactac cccgataagg gcaacgaccg agagggtccg
     7201 tgggggagat tttttggtta ggagcatatg tatgacacca atggttacgt aggcaccggt
     7261 agcaatgtca gttaaaagga gtgcacctga ggacccgctt gagatactca gtcgaaccag
     7321 ttggccaata attaaactca ccataataat aagaggaaag aggcgaattg aacggaacgc
     7381 tgcgaataac ccgagcaaaa caatgcctcc tgcagcggct gcaaatgcaa tacgagggtc
     7441 ctgagggacg aacaaaaaaa gaagagctcc taaagcgcag gccgctacca ccggaagaga
     7501 gaatcgctgg aaagatgtca tatgctgact attatagctg ttttttcccc gctatgctaa
     7561 agttgacagg aagcgactct aggagggctc cgtcatccag ttgagctagt aggcgaaact
     7621 gggtaccaac aggacctaga agcgctctct ctttataaac cctttactat gtctatccgt
     7681 actcatctca taacgcttca aaaatggtgg cttttcgccc ttctctttct cattcctttg
     7741 tccttgcaat acctcataaa aacgccggtt gctattagta ccttgaatat tttatcctta
     7801 ggatttatct ttctgggggt gctaaatgcc cttacgggtg gtgtaccgat tcgtcctgtt
     7861 cagcatcgct cgtatgtatg ggttatcctt ttgcttcttg gggccttggc gtatgctctc
     7921 ctctttaccc atcctctacg caacgcagta ggactgtgga cgagccgact gtctcaacct
     7981 ttattggtgg gatattttgc ctatcaacta ctcgctaacc gggttattac tctctctgaa
     8041 ccgattcgtg ctctgttcta ctcgctggtg cctctcctta ttttaggggg gatgcagctg
     8101 accgggtttt taccgtatgc cgataccgac cgagttactg cttcctactt ttttcctaat
     8161 acatttgctc gttatgttgg gattgtgctg cttatttcgc ttccttggat tcttttctca
     8221 atccgccagt ataagcgcct tcatttaggg ctttggattc ttggcgtact cttgcttttg
     8281 agttcgaagt cgtatggggg gaccgtggcg ttcttcggtg ggctcaccgc tctcttgttt
     8341 gctcttccaa atcc
//
LOCUS       DASWYB010000014         5260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_166789, whole genome shotgun sequence.
ACCESSION   DASWYB010000014 DASWYB010000000
VERSION     DASWYB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166789"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VGE59_00845"
     CDS             complement(<1..268)
                     /locus_tag="VGE59_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEY1074235.1"
                     /translation="MRTTVVPAQITSVEDRIAGNFTFAQVLLLIVPLLVGTAIYVGVE
                     PRSDLSTVKTILVCAQFLFFGALAIRYNGKILAQWLVVYFAFYLR"
     gene            complement(265..1533)
                     /locus_tag="VGE59_00850"
     CDS             complement(265..1533)
                     /locus_tag="VGE59_00850"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074236.1"
                     /translation="MKKVIPTLLLAGILLSPAGALAASNTEVANFTRDTLTILMSLAS
                     MAAVFFLIRGAYLYITSTGKPEALDEAKRTIRNALIGLAIVIGAGVFSSILNTAFSEP
                     SAGNLGTAINLSPILPAEETGSLTRILLDAVSGFLQNIIQSATQPIIDGITGFLTNTP
                     SLATNSVVFNFWLLMVGITDSLFAIVIAMLGLHVMSAASFGFEELSLKELLPRIGLAF
                     LLANTSIFLIDWVIALSQTMITAVLHSTGGLGQAWILNAFDPATLLSGGTVLITLIFM
                     VVFVLLAIVLLLFYISRLMLLAFGAVISPLIFLIWLMPRWTDFADSAIKAYMVTIFTL
                     FIHVVIIQLASAFLTVPDQVGTNPFISILIGIALFSILLKSTATTMQLALSSQTTGAL
                     KKFSGQLLNVLSPATAATKVRVISRHGRSA"
     gene            complement(1542..1883)
                     /locus_tag="VGE59_00855"
     CDS             complement(1542..1883)
                     /locus_tag="VGE59_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY1074237.1"
                     /translation="MQRLRSLFGALCLSLMVVALTTNPAFAAESADLSKVQNFIQNII
                     QVLVTLAGLLAAGFLVVGGIGYITSSGNPETLDRSKKTIIYAGLGLAIAIGAFVISNI
                     VTDLAKNAFGG"
     gene            2218..4779
                     /locus_tag="VGE59_00860"
     CDS             2218..4779
                     /locus_tag="VGE59_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEY1074238.1"
                     /translation="MITPIVIVLVALIVISILLTIVRNVVNRKEYAKTFLELTFPSDN
                     TKSAFATEQLYVLLHSRSKSQRIKERLLGQKKVYSFELVSTKDSGIRYIVSVPQKEAE
                     AFQRSMLSFLPDIKIREVEDYVSSEDQKVVRIAELKLSSDFVLPLLDQKALAEHDPMS
                     YLTGHMTKLALNELTAFQLVLTPVLPSLHRRIIQRTHELKRRIGQGLPLSPLIQNRSA
                     YMPNAIKVIFRSLAWLIDGAVRLVISLPSLIFDPTNKSIPILQSTVETHVKDSANPYE
                     KELGEIIKSKLDQHLFEVSLRIYVVANTEQAAQYRTDELVAAFRPFASPQQSIVVRKN
                     YLLSVRNQVRSFESRHLSVGLFIQNPILSSSELSDLYHFPHTKTTRTEDLIKSKSQEL
                     PAPLSLKNNHNLDVVFGVNNYGNSSSDVGLTDDDRSRHMYLIGQTGSGKSTVIYHMAK
                     DDIRKGRGLAVIDPHGDLAEDLVATVPESRIDDLIYFNPFDIKHPIGVNLLELTPDLD
                     EDELELEKELVCESVISIFRRVFNKDENTDAHRIEYILRNTIYTAFNVPDATIFTIYD
                     LLNDPEVRKKTIRTLTDENLINFWKQEFGKAGGYQVVKMVSGVTAKVGRFLFSPTAKR
                     ILEQPKSTIKFDEVLDKSKILICNLAEGKLGEDTSQLLGTMIIAKIQQAAMRRARNER
                     ATRTPFYLFVDEFQNFATSSFTKLLSGGRKFGLRVTIAEQSTAQQSDRNTVNVILANT
                     GTVVCFRTASPIDEELMLSQFSPLVQPGDIGNLPRYHFYMRLAAMEPTDAFSGETLPM
                     NAPKDEAKIEKLVEASRSNYAIVYQKPAPKEVVPDAKPKAKKALTVAQEAVDTLP"
     gene            4882..>5260
                     /locus_tag="VGE59_00865"
     CDS             4882..>5260
                     /locus_tag="VGE59_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEY1074239.1"
                     /translation="MTQLTQTTLTSRQLEILLLLYRFRFLTRSHIQTLLHHKNHAHIL
                     KWLNELTSQNYITQYYTKTIPASPAIYSLAKLGRTYLKAHTDSNTKHLSRLRRDPVYT
                     SKFRNHYLFLADIYISLLELTNKT"
BASE COUNT         1534 a         1228 c         1160 g         1338 t
ORIGIN      
        1 gacgaaggta gaaggcaaag tagacgacaa gccactgagc gagaattttg ccgttgtagc
       61 gaattgccaa tgcaccaaag aagaggaatt gagcgcacac tagtatggtt ttgacagtgc
      121 ttaaatccga tcgcggttct acgccgacgt aaatagcggt gccaacaaga agcggaacaa
      181 tcaggagtaa gacttgagca aaggtgaaat tgccggcgat ccggtcttcg actgatgtaa
      241 tctgagcggg aacaaccgtt gttctcatgc agacctcccg tgtcggctaa tgactcgtac
      301 tttagtagcg gccgtcgctg gacttaagac gttgagtaac tggccactga atttctttaa
      361 ggctccggtg gtttgtgatg acagggctag ctgcatcgtt gtagctgtgc tcttcaaaag
      421 gatggagaac agagcgatac caatgagaat cgagataaac ggattggtac cgacttggtc
      481 aggaacggtt aagaaggcgg aagccagttg aataatcact acatgaataa agagcgtaaa
      541 gattgtcacc atataggctt taatggcgct gtcggcaaag tctgtccaac gcggcatgag
      601 ccagataaga aagatgagtg gcgaaataac tgccccgaac gccagaagca ttaatcggct
      661 aatatagaac agcaatagaa cgatcgccaa caagacaaag acgaccatga agataagtgt
      721 aattaagact gtgccgccac tgagtaacgt tgctggatcg aaggcattaa gaatccaggc
      781 ttggccgagt cctcccgtcg agtgcaatac cgccgtaatc atggtctgag aaagggcaat
      841 aacccagtcg ataagaaaga ttgaggtatt ggcgagtaag aaggctaaac cgattcgagg
      901 taagagttct ttgagactta attcttcgaa tccgaaactg gcagcgctca tcacgtgcaa
      961 accaagcatg gcaatgacga tagcgaagag tgagtcagta atgccgacca ttagcagcca
     1021 gaagttaaag actactgagt ttgtcgcgag agatggtgtg tttgttaaga atccggtaat
     1081 cccatcgata atcggctgag ttgctgactg gataatgttt tgcagaaaac cggaaacggc
     1141 atccaggaga atacgggtca aagaaccagt ttcttcagcc ggaagaattg gactgaggtt
     1201 aatcgccgtt ccaagattgc cagcgcttgg ttctgaaaac gcggtattca agatggacga
     1261 gaacactcca gctccgataa cgatcgctag tccaataagt gcattgcgga tcgtccgttt
     1321 ggcttcatcc aacgcttccg gtttgccggt ggaagttata tacaaatacg ctccgcggat
     1381 cagaaagaac acggccgcca tagaggccag gctcatcaag atcgtcagtg tgtcacgcgt
     1441 aaagttagct acttcagtat tcgaagcagc gagtgcaccg gcgggagaca aaaggattcc
     1501 cgccaagagt aaggtgggaa tgaccttctt cataagtgct cctaaccgcc aaaagcgttt
     1561 ttagccaggt cggtcacaat gttgctgatg acgaaggctc caatagcgat ggccaaacca
     1621 agtccagcgt agatgatggt cttcttcgat cggtcgagag tctccggatt accggaacta
     1681 gtgatgtagc cgatgccgcc gacgactaaa aaaccggctg caagcaggcc ggccaacgtc
     1741 acaagtactt ggataatatt ttgaataaaa ttctgcacct tcgacaagtc ggcagattcc
     1801 gcggcaaacg caggattggt tgtcaaagcc acaaccatga gagataggca caaggcacca
     1861 aaaagggaac ggagtcgttg catgtataac ctcctcccct cccgacgaga cgctgccgcg
     1921 tgactcgtat gtatttgctt aattattaac tagttaagac tttaaaccta gctgaggcct
     1981 ttttatcatg tcgtctgata agctgtcaat agcttggttg gtttattaaa aatatattaa
     2041 attttattgt actcccttta cttctcctgt gtggttgtgt taaacctaac tcaataagga
     2101 gggcgatgat actttctcag gttatctgca ggagatacgc ccataaccga cctcttctca
     2161 ctttcgcata cttggacgct tagtgacctt aactcctttt ggtcgactat tttgctggtg
     2221 ataacaccta tagtaatcgt actcgttgca ctcatcgtaa tttctatact gctcacgatc
     2281 gtacgcaatg tcgtaaaccg aaaagagtac gctaagacat ttttagaatt aacattccct
     2341 tcggacaaca cgaaatccgc ttttgctaca gagcaattgt acgttctact gcatagccga
     2401 tctaagtcgc agcgaataaa ggagcgactg ctcgggcaaa agaaggttta ctcattcgag
     2461 ctagtttcaa caaaagacag tggtatccgg tatatcgtca gtgttcctca aaaagaagct
     2521 gaggcctttc aacggagcat gctttctttt ctgccagata taaagatccg agaagttgaa
     2581 gactatgttt catcagaaga tcagaaagtt gttcgcatcg ctgaactgaa gctatcgtca
     2641 gactttgtct tgccccttct tgatcaaaaa gcactcgcag aacatgaccc gatgtcatac
     2701 ctcactggtc atatgacaaa actggcattg aacgaactaa cagcatttca actggtctta
     2761 actccggtat tgccgtcatt gcatcgccgc atcatccagc gtacacacga actaaagcgt
     2821 agaatcggcc aaggattgcc attgtcgcca ctgattcaga atcgatcagc ttatatgccg
     2881 aatgctatca aggtaatctt ccggtcgttg gcatggctta ttgatggagc cgttaggtta
     2941 gtcatttctt tgccctcttt gatatttgat ccgacaaata aatcgattcc tattcttcaa
     3001 tcgacagttg aaacacacgt taaagacagc gccaatcctt acgaaaaaga acttggtgaa
     3061 ataattaaaa gcaagctcga tcagcacttg tttgaagtgt cgttacgcat ttacgtggtg
     3121 gcaaatacag aacaagcggc tcaatatcga accgatgaac ttgtcgcagc ttttcggcca
     3181 tttgctagcc cgcaacagtc gattgtcgtc cgaaaaaatt acttactatc cgttcgtaac
     3241 caagtaagat cattcgagtc acggcatttg tcagtaggtc tatttattca aaatccaatt
     3301 ctttctagct cagaactatc cgacctctac cacttcccac acacgaagac cactcgaacc
     3361 gaagatctaa ttaaatctaa aagccaagag ctgccagctc cgttgtcgtt aaaaaataac
     3421 cacaacctag atgtcgtatt tggcgtaaat aattacggta attcatcgag cgatgttggc
     3481 ctgacggacg atgaccgctc gcgtcacatg tacctaatcg ggcaaacggg ctcgggtaaa
     3541 tcgaccgtta tttaccacat ggcaaaagat gatattcgca aaggtcgtgg actagcggta
     3601 attgatcctc atggcgactt ggctgaagat ttagttgcta cagttccgga atctcgaata
     3661 gacgatctta tctacttcaa cccgtttgat atcaaacacc caataggcgt gaacttactt
     3721 gagctaacac ctgacctgga tgaagacgag ttggaacttg aaaaagaact tgtctgcgaa
     3781 agcgttatct caatcttccg tcgggtattt aacaaagacg agaatacgga tgcgcatcgt
     3841 atcgaataca ttcttcgcaa tacgatctat accgctttca atgtgccgga tgcgacaatt
     3901 ttcaccatct atgacttact taacgatcct gaagttcgca agaaaacgat tcgcaccctg
     3961 actgatgaaa atctcattaa tttttggaaa caggaattcg gtaaagccgg tggctatcaa
     4021 gtcgtaaaga tggttagtgg cgttaccgct aaagttggac gatttttatt ctcgccaacg
     4081 gctaagagga ttttagagca accaaagtcc actattaagt tcgatgaagt tctagataaa
     4141 agtaagatcc ttatttgtaa tttagctgaa ggtaagctcg gcgaagacac gtcacaactt
     4201 ttaggcacga tgattattgc caagattcaa caagctgcta tgcgtcgagc cagaaacgaa
     4261 cgtgctaccc gtacaccgtt ctatcttttc gtcgatgagt tccaaaactt tgctaccagc
     4321 tcgtttacga agcttctttc cggcggccgc aaattcggct tgcgcgttac tattgctgag
     4381 cagtctactg cgcagcagtc agatcggaat actgtaaacg ttattctggc caataccggc
     4441 acggttgttt gtttccgtac cgctagccca attgacgaag aactcatgct gtctcaattc
     4501 tcaccgcttg tacagcctgg cgacatcggc aatttaccgc gttatcactt ttatatgcgt
     4561 ctcgcggcaa tggagccgac tgatgcattc tccggcgaaa ccttgccgat gaacgctcct
     4621 aaagatgagg caaagatcga aaagttagta gaggcctcgc gtagtaatta cgctatcgtc
     4681 tatcaaaaac ctgcgccgaa agaagttgtt ccagatgcta aaccaaaggc gaaaaaggct
     4741 ctgacggtag ctcaagaggc agttgatacc ctgccgtaaa agctatcagc cgtatgatag
     4801 tattttccgc gctactacag gtttagaaaa taaaataacg tacgcttata tttatatatt
     4861 atatttctct cttctacgct tatgacccag cttacccaaa caactctcac atctcgacaa
     4921 ctcgaaatat tactcctgct ttaccgcttc cgctttttaa cacgctctca tatccaaacc
     4981 ctcctacacc acaagaacca cgcacacatc ctcaaatggc ttaacgaact tacatcccaa
     5041 aactacatca ctcagtacta cacgaaaacc attccagctt ctccagctat ctactcacta
     5101 gccaaactag gccgtaccta tctcaaagcc catacagaca gtaatacgaa acatctcagt
     5161 cggttaagga gggatccggt ctatacgtca aagtttcgca accattatct ctttctggct
     5221 gacatctata tttcgctgct tgaactgaca aataaaacta
//
LOCUS       DASWYB010000015         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_218118, whole genome shotgun sequence.
ACCESSION   DASWYB010000015 DASWYB010000000
VERSION     DASWYB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218118"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VGE59_00870"
     CDS             <1..583
                     /locus_tag="VGE59_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1074240.1"
                     /translation="KDAIERKLSHKMVLFYSNRRPEDAPYLAELEILRQQNPNLTLVA
                     TMTEPEKSAQVWKGETGIINQAMIRKYVSDLERPIYYIAGLSEMVAAMKSLLSTLGID
                     EANVRAEDFSAVKMQIMTLMPGSWRNYAIPAAISIVVILFIFIHVGATISLYNTALLQ
                     NISYLTLGFIVVIFAVKIFAVVKLKHHLTQKRK"
     gene            594..2756
                     /locus_tag="VGE59_00875"
     CDS             594..2756
                     /locus_tag="VGE59_00875"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEY1074241.1"
                     /translation="MQHEHKHHNHQAPLAKETKCELCGMIAEHMVKDQHGVTHHYCSH
                     HLPQASHSAHAEHDGHSEHDKHAGHNPDMFRDRFWLSLALTIPVIFYSHMIQMWLGFT
                     APTFPGSQWLSFVLGTIIFFYGGTVFVKSALAEIKAKLPGMMTLISLAIIAAYGYSVA
                     TTFFIKGSEFFWELATLITIMLLGHWIEMKSVASAQGALKELAKLLPDEAELVQGRET
                     KKVAIAELKASDIVLVRPGAKIPADGEIVEGESTVNEAMITGESKPILKTVGSAVIAG
                     TINESGSLKVKVTKIGENTALAGIMRLVAEAQASHSKAQILADKAAFYLTFIALIAGV
                     GALVGWLTAGAGVNFALESMVGVLIIACPHALGLAVPLVTAISTSKAAQNGLLVRQRV
                     ALEAARNIDVVLFDKTGTLTKGEQGLSDVYSAGKVAPDEILSLIASVEAPSEHSIGRA
                     LVAAAKEKKVKLTDVTGFSALKGKGVTAKVGNDTYFVGGPRMLAEQKAALPTSLEAKI
                     KAADEAGKTIIYLIKEKNIFGAVTIADTVRPESKEAIQSLHNLGVRVAMLTGDSQGVA
                     KWVAKELGVTEYFAEVLPEHKVDKVKELQKDGSKVAMVGDGVNDAPALTQANVGIAIG
                     AGTDVAIESAGIILVKSDPRDVVKIIRLSKATYKKMVQNLVWATGYNVIAIPLAAGIL
                     APWGLILPPALGAGLMSISTVVVALNAQLLRRLQLDNV"
     gene            2771..3175
                     /locus_tag="VGE59_00880"
     CDS             2771..3175
                     /locus_tag="VGE59_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074242.1"
                     /translation="MSKRIIHFTIAITGLIFVATSLLGLASSMQMASDTKEHTCLLMP
                     TEPNCDMTIAEHISLWQSTFTSVASSIFTPLLILLFITVVGTITFFLTRDKDSKPPGF
                     WRDDPESNLFNNLVRAFAQGLIHPKIYPSLTA"
     gene            3214..>3634
                     /locus_tag="VGE59_00885"
     CDS             3214..>3634
                     /locus_tag="VGE59_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074243.1"
                     /translation="MDKGVVIIIGITVVILGSIIGITMNNPQQKLTNSFTGEDNDTIS
                     EQGVHYHPELSIVINGQKQEIPANIGVGAQYASNKFYDPMMKMTDIHTHDPGGVLHWE
                     VMDGPVKKGHVKLGSFFNVWGKTFTKDQIFEAKNADSG"
BASE COUNT         1003 a          843 c          851 g          937 t
ORIGIN      
        1 taaagatgct attgagcgaa aactatcgca taagatggtt cttttctatt caaaccgacg
       61 accagaagat gcaccctatt tagctgaact cgaaatactc cgtcagcaaa atccaaacct
      121 taccttggtg gcaacgatga ctgagcctga gaaatcagcg caggtatgga agggcgaaac
      181 cggaatcatc aatcaagcga tgatcaggaa atatgtgagt gatttggaac ggcccatcta
      241 ttacattgct gggctatcgg aaatggttgc ggcaatgaaa tccttgctca gcacattagg
      301 cattgacgaa gcaaacgtcc gcgccgagga cttttctgcg gtcaagatgc aaatcatgac
      361 tctgatgcca ggctcatggc gaaattacgc tatccctgct gcaatttcga ttgtagttat
      421 tctttttatc tttatacacg tcggtgctac tatctcgctt tataacactg ccctccttca
      481 aaatatttct tacctcactc ttggcttcat tgtcgttatc ttcgctgtta aaatctttgc
      541 cgtcgttaag cttaagcatc acctcacgca aaagagaaaa taagggagca attatgcagc
      601 acgaacataa gcaccacaac catcaggcac ctctagctaa agaaacaaag tgtgagcttt
      661 gcggtatgat cgctgaacac atggttaaag atcaacacgg cgtcacccat cattactgct
      721 cccatcactt accacaagcc agtcattcag ctcacgcaga acatgatgga catagcgagc
      781 acgataaaca cgctggccac aaccccgata tgtttcgtga ccgtttctgg cttagcctag
      841 ccttaaccat tccggtcatc ttttactctc atatgatcca aatgtggctt ggctttacgg
      901 cgccaacctt tcccggctca cagtggctct catttgtcct cggtacaatc atattctttt
      961 acggtgggac agtttttgtt aagagcgcac ttgctgaaat aaaagctaag ctgccgggca
     1021 tgatgactct tatttcgctc gctattatcg cggcctatgg ctatagcgtc gccacaactt
     1081 tcttcattaa gggatcggag ttcttttggg aacttgcaac gcttatcact atcatgctgc
     1141 tgggtcactg gattgagatg aaatcagtcg ccagtgccca aggtgcgcta aaagaactag
     1201 cgaagcttct tccagacgag gcggagcttg ttcaaggcag ggaaacgaaa aaagtagcca
     1261 tagcagagct taaagctagc gatattgtgc tcgttcgtcc cggtgctaag ataccggctg
     1321 atggggaaat cgttgaaggg gaatcaacgg ttaatgaagc catgattacc ggcgaatcaa
     1381 agcctatact gaaaacggtc ggtagtgcgg ttattgctgg taccattaat gaatcggggt
     1441 cactaaaggt aaaagttaca aagatcggtg agaatacagc cttagctggc ataatgcggc
     1501 ttgttgccga agcacaagct tcacactcaa aagcccaaat cctcgccgac aaagctgcct
     1561 tttatcttac ctttatcgcc ttaattgccg gtgttggcgc acttgtcggg tggctgaccg
     1621 caggagctgg agtcaacttc gcgttagaga gcatggttgg cgtccttatt atagcctgtc
     1681 cccacgctct tggtttagcg gtaccacttg tgacggctat atcgacctca aaggctgctc
     1741 aaaatgggtt gctcgttcgt cagcgcgtgg ctctggaagc agcccgtaat atcgatgtag
     1801 tactttttga taaaaccggc accctcacga aaggcgaaca aggcctcagc gatgtctatt
     1861 ctgcaggaaa ggtagcgcca gatgagatcc tcagcctcat tgcttctgtc gaagcaccca
     1921 gtgaacactc aatcggtcgg gccttagtgg ccgccgcaaa agaaaagaag gtcaagctga
     1981 ccgatgtaac cggctttagt gctcttaagg ggaaaggagt cacggccaag gtcgggaacg
     2041 atacctactt tgtcggcgga ccaagaatgt tggctgagca gaaagccgct ctacctacaa
     2101 gcttagaggc aaaaattaag gcagccgatg aagcgggtaa gacaattatt tatctcatta
     2161 aagagaagaa tatcttcggt gccgtcacga ttgctgacac ggttcgccct gaatcaaaag
     2221 aagccattca gtcacttcac aacctcggtg ttcgtgtagc tatgttgacg ggcgactcgc
     2281 aaggtgttgc caaatgggta gcgaaggagc tgggggttac ggaatacttt gcggaagttt
     2341 tacccgaaca taaggtcgac aaagtaaaag aactccagaa agatgggagc aaagtcgcga
     2401 tggttggcga cggcgttaat gacgctccgg ctctgacgca agcgaatgtt ggtatagcga
     2461 ttggggctgg aaccgatgtc gccattgaat ctgctggtat tatcttagtc aagagcgatc
     2521 cgagagacgt ggtaaagatc attcgactct ctaaagcaac atacaaaaag atggttcaaa
     2581 accttgtctg ggcaacggga tataacgtta ttgcaatccc actagccgca ggcatcttag
     2641 ctccctgggg ccttatcttg ccacctgccc ttggtgcagg acttatgtct attagtaccg
     2701 tggttgttgc gctgaatgcc caattattac gtcgacttca actcgataat gtgtaaagat
     2761 aagggaagtt atgagcaaac gtattattca tttcactatc gcaattactg gactcatatt
     2821 tgttgcgacg agtctactcg gattagcctc ctcgatgcaa atggcatcag acacaaaaga
     2881 gcatacgtgc ctgctcatgc ctacagagcc taactgcgat atgaccatag ctgaacatat
     2941 ttcgctttgg cagagtacct ttacgtccgt agcttcaagt atctttaccc ctttgttaat
     3001 tcttctcttt attacagtag ttggaacgat tacctttttc ttgacacgag acaaagactc
     3061 aaagccgccg ggtttttggc gcgacgatcc cgaaagtaac ttatttaaca atcttgtgag
     3121 agcctttgct caaggcctta ttcatcctaa aatctatccc tcgcttactg cttagcacta
     3181 tagttggtac acataaaaaa tatgaggagc gatatggata aaggcgtagt cattattatt
     3241 ggtattaccg ttgtcatttt aggcagcatc attgggatta cgatgaataa tccgcaacag
     3301 aaattgacga actcatttac gggcgaggat aacgatacta tttccgagca gggagtacac
     3361 tatcatcccg aactatcgat tgttattaat gggcaaaaac aagagatacc agctaatata
     3421 ggagtcggcg cccaatatgc cagcaacaag ttctacgacc caatgatgaa aatgaccgac
     3481 attcacacgc acgatcctgg cggggttctg cactgggaag tcatggatgg gcctgttaag
     3541 aaaggtcatg ttaaactagg ttcattcttc aacgtttggg gtaaaacttt cactaaggat
     3601 caaatctttg aggccaaaaa cgccgacagt ggag
//
LOCUS       DASWYB010000016         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_10380, whole genome shotgun sequence.
ACCESSION   DASWYB010000016 DASWYB010000000
VERSION     DASWYB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10380"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VGE59_00890"
     CDS             complement(<1..415)
                     /locus_tag="VGE59_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074244.1"
                     /translation="MTSNRTLSGSAIALAVLGIAAVATFLLGIVQIFPQQVRESQRIV
                     ARDQAEYAAWAGAEHALLELKRSRGESKRFELSAEQQKDPTRGMPYVLLSQKQAACLV
                     NGCLSYRQLLGSLKNASPVSFPLGNDKSAAHYDLLV"
     gene            complement(412..1146)
                     /locus_tag="VGE59_00895"
     CDS             complement(412..1146)
                     /locus_tag="VGE59_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1074245.1"
                     /translation="MKKNAGYTLLELLLTIVVFSAIMGALFSAFLALRQSDTVRAHDS
                     LTIQLAQLAFSPMVSSLKEAEGSEQVKVLNREGEFVCQTVHGFYLNGTQGVKISPPEV
                     MLDLGDTLVALKVEETTNAAGLPTYEWVRYEYSLETKQVKSDPVTMLLQRKYRVFPTE
                     SFQWPNLLSSCDITQLPWDRLPSHVQEKQLTPSDMSVQSLHLRLNSSDAKEPRFASVT
                     FTVLRDGKTVTLHTTVGSSYVQRDAR"
     gene            complement(1143..1817)
                     /locus_tag="VGE59_00900"
     CDS             complement(1143..1817)
                     /locus_tag="VGE59_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074246.1"
                     /translation="MVTNRMHGFSLIEIVVSGALIATTLGALFAVSSQVRKLDEVHSD
                     RVVASGFAREGIEIVRSLRDAQAARQTCEGGVCDDWRVGLIASSGITPQRAVEKLPLA
                     KPIVATEHGYELGDSVVFTHERPCTDFLTINEKGSVVVSEQEPVTNKSVWCRRLLLEP
                     VSLTSGEESTDSMRVRSQVAWKTSPQQSWTVMGSLQPENPCSSHSTTSEWCLEYVTYL
                     TDWKSL"
     gene            complement(1804..2565)
                     /locus_tag="VGE59_00905"
     CDS             complement(1804..2565)
                     /locus_tag="VGE59_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074247.1"
                     /translation="MGFFSICFNYGESYKEDRSIVIGGDRSRMIGASSTDFLLASVII
                     GVVLVIGIPIFRTLQRRQMLARTTQSIQQLISGVHELSIQESTTSGNGGRTIGYSLII
                     EQGSKGSPSFLACGQQSYTSARIGIVRLVYIEDDQSVRPELVTTDGQRLCSSTGAREK
                     IFTLPNAYALQVEEKNPHTLWFVPVVKDSEASLFSQVRQLVATYKGDDPLASNESLTL
                     YISHPASGECRSLVIWHDASGLLSTPQEGCPHGNK"
     gene            complement(2522..3367)
                     /locus_tag="VGE59_00910"
     CDS             complement(2522..3367)
                     /locus_tag="VGE59_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY1074248.1"
                     /translation="MTMNSELFILFVLGLGVGSFLTLVSNRIDTAESILVGRSHCNHC
                     KEILRWWELVPFVSFVLLQGRCIRCKKQIPPLYPFFELITALTFVGLRLAYPATPNYW
                     IFGGELVFVSLLLILFFYDVVHQAFPTSVLFIALGWAVIISIVKELASAPLHLGSSLQ
                     AAGWWFLAEPSHHLLSSLAGGIVGAGLLGLIAFPSKGLWMGYGDVILAGILGIWLGYP
                     FIIITILAAFYSGAIIGGIQLATKRLRPDHRIAFGPFLIVGALITTVWGDGILLYLFQ
                     LWGIV"
     gene            complement(3369..3851)
                     /locus_tag="VGE59_00915"
     CDS             complement(3369..3851)
                     /locus_tag="VGE59_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074249.1"
                     /translation="MTREKTLFQSGSIMLEVVAAVVVAAVLIGIGYMGIGGAIHRSRD
                     AKRQNDLVNIKQALELYYTDNQAYPKELESLKTNGYIREVPRDPKFKDDQKQPATYGY
                     MYEDAAQPQHYALYTRLEYKRAKQTLTGISNCDAPVTEKGNGVVNPTGKQACFRFTND
                     "
     gene            complement(3896..>4901)
                     /locus_tag="VGE59_00920"
     CDS             complement(3896..>4901)
                     /locus_tag="VGE59_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1074250.1"
                     /translation="FFRQMAIMVASGIPFITALTLASEQVESPLLQGILHTMGRDINN
                     GVPFSEAASRFPVVFDEVTVSLLKSGEATGQMQQVLGDLADHTEKSAQFGTRVRAAFL
                     YPTFVVAIMVVVGIIMTVVVVPRLKDSFADTSFTLPVTTQVLVSLSNALIHYWYLIAA
                     GLLMIFLLLRSYFVSVGGHRVLFSIQTTTPFLRQVTFHSYLVRFASLLAMLIRAAVPL
                     PEALRIVGDSFENHIWAKTINSVRQEVERGVPLSAALSRHPIFPEPLVQMIGVGEQTG
                     QMEATLTNMANFYEDQTNASIKALATLIEPVALVVVSLAVGFVVLAVILPIYSITQQL
                     "
BASE COUNT         1425 a         1237 c         1074 g         1165 t
ORIGIN      
        1 atacgagcag atcataatgt gcagcacttt tgtcattccc taagggaaat gaaacagggc
       61 tcgcattttt aagagaaccc aagagctgcc gatacgacaa acatccattc acaaggcacg
      121 ccgcttgctt ttgagaaagg agtacgtacg gcatccctcg ggtaggatcc ttctgctgct
      181 cggcagacaa ctcaaaccgt tttgattccc cccgtgatcg ctttaactca aggagcgcat
      241 gttcagcacc tgcccatgcc gcgtattcag cttgatctct tgcaactatt cgctgactct
      301 ctcgcacttg ttgcggaaaa atttgtacaa ttcctaacaa gaatgtcgcg actgccgcaa
      361 tacctaacac cgccaaagca attgctgatc cagacaatgt tcggttagaa gtcatcgcgc
      421 atccctttgt acatatgagc tccctacggt tgtgtgaagc gtaactgttt tgccgtcccg
      481 tagaacggta aacgtaacac tcgcaaaccg aggttctttt gcatcagaag aatttaaccg
      541 aagatgtagc gactgtacag acatgtccga cggggtaagc tgtttctcct ggacatggct
      601 tggcaaccga tcccatggca gttgcgtaat atcacagctc gaaagaaggt ttggccactg
      661 gaaggattct gtgggaaaca cacggtactt tcgttgtaaa agcattgtaa ccgggtcaga
      721 cttaacctgc ttcgtttcta gcgagtattc ataacgaacc cattcatagg ttggtaaacc
      781 agcggcattt gtcgtctcct cgactttgag agccactaaa gtatcgccca aatctaacat
      841 aacttctggg ggagaaatct tcactccctg tgtgccatta agatagaaac catggaccgt
      901 ttgacacacg aactcccctt ctcggtttaa aactttcact tgttcactcc cctcagcttc
      961 ttttaatgaa ctaaccatgg gagaaaaagc gagttgagcc agctggattg tcagactgtc
     1021 gtgggcgcga actgtatcac tttgccgtag tgctaaaaaa gcggaaaaca aagctcccat
     1081 gatggccgag aagaccacaa tcgtcagtaa gagttctaga agggtgtacc ctgcgttctt
     1141 tttcataggc tcttccaatc agtaaggtaa gtaacatact caagacacca ttctgaagtt
     1201 gtggaatggg aagaacaggg gttttctggc tgcagtgagc ccatcacggt ccacgactgt
     1261 tgtggcgacg tcttccaggc aacttgtgat cggactctca tactatcagt actttcttcc
     1321 ccgcttgtga gagaaactgg ctcaagcaga agccgacgac accacactga cttattcgtt
     1381 acaggctctt gctctgaaac aacaacactt cccttttcat ttatcgtaag gaaatccgta
     1441 cacggccgct cgtgagtaaa gacaacagag tctcccagct catacccatg ttcggttgcc
     1501 acaatcggtt tcgccagagg caatttctct acagcccgct gaggcgtgat ccctgatgaa
     1561 gcaatcagcc ccacccgcca atcatcacat actccccctt cacacgtttg gcgagctgct
     1621 tgagcgtcac gcaaactccg tacaatttcg attccttctc gagcaaaacc actggcgaca
     1681 acccggtcac tatggacttc atcgagcttg cgcacttggg aagatactgc aaatagagca
     1741 cctaaggttg tcgcaattaa agcacctgaa acaactattt caataaggct aaacccatgc
     1801 attctatttg ttaccatggg gacatccttc ttgcggcgta cttaagagac cagatgcatc
     1861 gtgccagatt acaagcgatc ggcattctcc tgaagctggg tgagagatgt acaaggtcag
     1921 cgattcgtta cttgccagtg ggtcatcgcc cttgtacgtg gcgaccaatt gccttacctg
     1981 ggaaaagagg gatgcttctg agtctttcac aacagggaca aaccataacg tatggggatt
     2041 cttttcttcc acttgaagtg cgtatgcatt aggcagggta aaaatcttct cccgagcacc
     2101 cgtggaagag cacaatcgct gcccgtcggt agtaacgagc tccggacgaa ctgattgatc
     2161 atcctcgatg tataccaatc ttacaattcc aatgcgcgct gaggtatatg actgctgacc
     2221 gcacgccaga aacgagggag agccttttga tccctgttca ataatcaagg aataccctat
     2281 ggtacgcccc ccattcccac ttgtagtgga ttcctgtatc gacaactcgt gcactcctga
     2341 tatgagctgt tggatcgatt gagttgtacg agcaagcatc tggcgacgct gcaacgtgcg
     2401 gaagatagga atccctatta ccaatacaac cccaataatt acggaagcga gtaggaaatc
     2461 cgtagagctg gcaccaatca ttcgagaacg atccccacca atcacaatgc ttcgatcctc
     2521 cttatacgat tccccatagt tgaaacagat agagaagaat cccatctccc caaacggtcg
     2581 ttatcaaagc acctactatt aaaaacggac caaaggcaat acgatgatca ggtcgcagcc
     2641 gcttggtggc gagctgaata ccaccgatga tagccccact ataaaatgca gctaaaatag
     2701 taataatgat aaacggatat cctaaccaaa tacctaaaat gcctgctaat ataacatctc
     2761 catagcccat ccataacccc tttgacggga atgctatcag tccgagcaat ccggcgccca
     2821 caatgccacc ggcaagacta gagagaagat gatggcttgg ttctgccaaa aaccaccaac
     2881 cagccgcttg gagtgatgat cctagatgca agggagcact ggctaattcc tttacaatag
     2941 aaatgataac agcccacccc agagcaataa aaagaacaga ggtagggaaa gcctgatgga
     3001 ccacatcata gaaaaacagg ataagaagca acgaaacaaa gacgagctcc cctccaaaaa
     3061 tccagtagtt aggagttgca ggatatgcta gccgaagtcc tacaaatgtg agggcggtaa
     3121 taagttcaaa aaacggataa aggggtggaa tttgcttttt acaacgaata caccgtcctt
     3181 gcaaaagaac aaaactcaca aaggggacaa gctcccacca acgaagaatt tctttacagt
     3241 gattgcagtg agaccgccct accaatatcg actcggccgt atcaatccga ttactgacaa
     3301 gcgttaaaaa cgatcctacg cctaagccca gtacaaagag aataaaaagt tcagagttca
     3361 tcgtcatatt agtcattcgt gaagcggaaa cacgcttgtt tacccgtagg attaaccacg
     3421 ccattacctt tttcggtaac aggagcatca cagttcgaaa taccagtgag cgtttgtttt
     3481 gcacgtttat attctaaacg ggtatagagc gcgtaatgct gtggctgtgc cgcatcttca
     3541 tacatataac cataggtagc aggctgcttt tgatcgtctt taaatttagg gtcacgggga
     3601 acctctcgga tataaccatt ggttttaaga gattcgagct ctttggggta cgcctggtta
     3661 tctgtgtaat aaagttctag ggcttgcttg atgttcacta aatcgttttg ccgtttagcg
     3721 tcgcgagaac gatgaatcgc tccgccaatt cccatataac cgatcccaat gagcaccgct
     3781 gcaacaacga cagctgccac aacttcgagc ataatcgagc cagattgaaa tagtgttttt
     3841 tctcgtgtca tatcttctcc atactagcct acctctccct agattactag cggcgctaga
     3901 gctgttgtgt aatcgagtat atgggcagaa taaccgcaag tacgacaaag ccaactgcga
     3961 gagagacaac aacaagcgcg actggctcaa tgagggtcgc taaagcctta atggaagcat
     4021 tcgtttgatc ttcataaaag ttcgccatat tggtcaaagt ggcttccatt tgacccgttt
     4081 gttcccctac tccaatcatc tgaactaatg gttcaggaaa aatgggatga cgagaaagag
     4141 ccgccgatag aggtactcca cgctctactt cctggcgcac actgttgatt gttttcgccc
     4201 agatatgatt ttcaaatgag tctcccacaa tccgcaaagc ctcagggagt ggaacggcag
     4261 cacggattaa cattgctaag agcgatgcaa agcgcacaag ataactatga aacgtcactt
     4321 gccgaagaaa tggtgtcgtt gtttggatcg aaaagagcac tctgtgccct cctactgaaa
     4381 cgaaatacga acgaagaagt aaaaatatca ttagtagccc agccgcaatt agataccagt
     4441 agtgaatcaa agcattactc aacgacacta atacctgtgt cgtgacaggg agagtgaatg
     4501 aggtatcagc aaaagaatct ttcaaacgtg gcacaaccac aactgtcata ataataccaa
     4561 ccactaccat aatagccacc acaaaggtcg ggtagagaaa agctgcccga acacgggtgc
     4621 caaactgggc actcttttcc gtatgatccg caagatcccc caagacttgc tgcatttgac
     4681 cagttgcttc cccactttta agcagagata ccgtaacttc atcgaagaca accgggaaac
     4741 gagacgcagc ttccgaaaaa gggaccccat tattaatatc ccgtcccatc gtgtggagaa
     4801 tcccctgcaa aagtggactc tctacctgct ctgaggcaag cgtgagagcg gtgataaaag
     4861 gaatgccgct cgccaccata atcgccatct gacgaaaaaa g
//
LOCUS       DASWYB010000017        15923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_61167, whole genome shotgun sequence.
ACCESSION   DASWYB010000017 DASWYB010000000
VERSION     DASWYB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15923
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61167"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VGE59_00925"
     CDS             <1..260
                     /locus_tag="VGE59_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074251.1"
                     /translation="FAFYLRPRFYIFTKNDPVHRTIVLPETKQEELVIPATIKLEQVR
                     QQLSFDEQMRIDRLLESDSLTLSFELAKKGGIDVSLVPIKD"
     gene            232..849
                     /locus_tag="VGE59_00930"
     CDS             232..849
                     /locus_tag="VGE59_00930"
                     /inference="COORDINATES: protein motif:HMM:NF022580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HEY1074252.1"
                     /translation="MSRLSQSKTKGSARTQIGIKEVRDNILILPDNRYRMILTTSSLN
                     FELQSEAEQDALMDSFQSFLNSITTPLQILIRVRELDIERYLEDFQASRANETEAIYK
                     KQLANYSEFIRKLVAGNKILSRRFYVVIPYENRGITDFATIKEQLMLEQEIIIKGLEK
                     LGMTARPLSSLEILDLFYSFYQPEQAKTQPLAQALVRQSNAFPHI"
     gene            851..2620
                     /locus_tag="VGE59_00935"
     CDS             851..2620
                     /locus_tag="VGE59_00935"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1074253.1"
                     /translation="MKTKLNKIRDERWQRKQTLEFHVGDQDQLDLITYSGLEEQRNYL
                     VIDNRYVRTLFVSGYPYTASSGWLNQLINFNYNVDISYHIEPIEATLALPKLNRKITE
                     LTSTKRAMERAGKIIGAELMDPLESAIELRDKIQRGQENLFQVSIYTTLSADSLVELE
                     KTTKLLETVLATRLFYTKYATFQQVEGLQSVLPRGDNLLAQKRNLDSSSTALTFPFIS
                     SELVHESGILYGVNRSNSSLVILDRFALPNANSILFAQSGAGKSYTAKVEILRNLMRG
                     TKVIVIDPEREYRQLAESVKGTYVKLSVHSKEKINPFDLSINAFTEADSFADHVQSLT
                     EIISLMAEGLDAEEKSVVDQAILKTYKKAGWSSKKSRKRDSANYPVLYDFYATLKSLK
                     QHKLCRRLEKYTTGSLAEVFEEQTNIQLDNRLVVFDTKDLPESLREIMHLVVAQFVQN
                     QVKSEPCKRLLVIDEAWLMLQHEESARFIAGLVRRARKYYLGVSIISQQANDFLQNAY
                     GRAIASQSALRILMRQDTTTIRTVAEEFRLSEYEQKFLLTCERGEALIVADQHHVVVK
                     ITASEDEHPLITTDPAENYRPKK"
     gene            2617..3243
                     /locus_tag="VGE59_00940"
     CDS             2617..3243
                     /locus_tag="VGE59_00940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY1074254.1"
                     /translation="MTPQTISAIATAFSMKKEIKYVFITFLVICLIPVFTVIMITEVG
                     LEIVSGALVSRDPQSVQVDIRDPRTGEVVDHIESLGVWPVSGPISLEFGEIDLPYQPF
                     HTGIDIATFTRTVGDPVVAFMAGKVTYADSVSWGYGKHVKIDHGHFTSSVYAHLDSLN
                     VAVGDEVEIGQVIGTRGSTGWSTGPHLHFEIRVFGIPVNPRVFLNGDP"
     gene            complement(3253..3735)
                     /locus_tag="VGE59_00945"
     CDS             complement(3253..3735)
                     /locus_tag="VGE59_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074255.1"
                     /translation="MKNEIERKFFIKELPDLSAREKIEYERYFLYNADGIKLRVQRKG
                     ERFELERKINVSEVERSREKIDVSEEEFALLKSLAKQVIIRDSYIVNDSPSITIKIYH
                     EKFEGLMRAEVEFDSAQELENFVPLAWFGTEITASPLARDADLLKLSEEKFLSLLAQG
                     "
     gene            3885..4100
                     /locus_tag="VGE59_00950"
     CDS             3885..4100
                     /locus_tag="VGE59_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074256.1"
                     /translation="MNLDLFQPDSVQKTGSSATENKFGSQEIISLKECKQYVGEFQLS
                     DQRIIEIRNNMIGIIDQVINAYLRNVG"
     gene            complement(4408..4647)
                     /locus_tag="VGE59_00955"
     CDS             complement(4408..4647)
                     /locus_tag="VGE59_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074257.1"
                     /translation="MNWYHEFAVFAILFELISDWWVKTPISTLNSSFHLLTYLSPVLF
                     TLKLVLSGKYRFYKFTFCTIVELVVKAAEFSLSSS"
     gene            complement(5615..6001)
                     /gene="ssrA"
                     /locus_tag="VGE59_00960"
     tmRNA           complement(5615..6001)
                     /gene="ssrA"
                     /locus_tag="VGE59_00960"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6123..6314
                     /locus_tag="VGE59_00965"
     CDS             6123..6314
                     /locus_tag="VGE59_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074258.1"
                     /translation="MELTIVPGGKKAKKQDPFIELCDGVIHSSTRIPPRTHVGPVIDR
                     RRRKKLPENVVDIRDRFKR"
     gene            complement(6378..7379)
                     /locus_tag="VGE59_00970"
     CDS             complement(6378..7379)
                     /locus_tag="VGE59_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY1074259.1"
                     /translation="MYDFVAIGDATLDVFMQLDDANVMCDIDKHNCKLCLNYGDKIAA
                     KRTDFIIGGNAANAAVGARRLNLSSAFMGTIGNDDTGKQVQNVFHTEGVSTEYLTIEK
                     DQKTNYSVVINFQGERTILLNHIPRTYHWNIAQAPRWIYLTSMGEGFEAIYDKVIGLV
                     REKHTMLAYNPGTHQLKKGLAFLKPSIAAATLFFVNKEEAILLLGADTHITMSELLKR
                     VKALGPEVVVITDGPKGSYVYNGSEMYQLDIFDGPVIERTGCGDSFATAFTVAFAEGK
                     TIPEAMLWGNANSTSVLAHIGPQAGLLDRAGIEKLIAKNPTIQPKVLLAASATVVHS"
     gene            7495..8151
                     /locus_tag="VGE59_00975"
     CDS             7495..8151
                     /locus_tag="VGE59_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074260.1"
                     /translation="MSMLHLRLKQLRRERNMSQEDLARKLGVSRQALIAIEQGASLPS
                     LRVIMALIDALEVTFNDLFGPAWSPFRSSDSDTESESSQLSLYRYQERGKTLPVTMHE
                     DSATFHIYAELAGVQEEDLTVDLSTQHVLIVAIKRSCPPPSNDLLMHIEEINFGPLLR
                     VIAMPSPIAADQARAEFKSGLLHLTLPKLQPEVSRRITFKKETIRPDKIHPKEEYGSE
                     "
     gene            8138..10798
                     /gene="clpB"
                     /locus_tag="VGE59_00980"
     CDS             8138..10798
                     /gene="clpB"
                     /locus_tag="VGE59_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY1074261.1"
                     /translation="MDPNKFTTKAQQALESAFQAATAHGQAADVTHLLISLLQQTEGT
                     TRTILTILEKPIDRILEETEDSRRHAPAQSPFSNTMGQVMITQDLGRVLSQAKQEAES
                     LTDEFISTEHLLIALTQVKTAAQTILDKYGIQREAILRALASIRGGERITDQNPEDKY
                     QALDKYGRDLTKLARENKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVSGDVPNSLKNKRVISLDLGAMIAGSKLRGEFEDRLKAVLNEVNRSNGQVI
                     LFIDELHTLIGAGAAEGAVDAANLLKPALARGELHAIGATTLKEYQKHIEKDAALERR
                     FQPVYVKEPNVEDTTAILRGLKEKYEIHHGVRIKDSAIVAAATLANRYIADRFLPDKA
                     VDLLDEATSALRLEIDSMPTEIDQTKRRIMRLEIEKEALKNEKEDTARKRLRELEKEL
                     ADLKETMVKLEGRWQQEKEALDRIHSANEEIEQAKAEASLYERRGEFSKVAEIQYKRI
                     PELEKQLTEAQQRLAAIPPQERMLQDAISEEQIATVVSRWTGIPVSKMLQSEQRKLFE
                     LEQQLAERVIGQREALEAVSNAIRRSRTGISDPNRPIGSFIFLGPTGVGKTETAKALA
                     STMFDSESAIIRVDMSEYREAHSVSKMIGSPPGYVGFEEGGQLTEQVRRKPYSVILFD
                     EIEKAHPEVFNILLQILDDGRLTDSKGRVVNFTNTLIIMTSNIGSSAISDYTERGNTK
                     RIGYIPNNDPEASEKQMEESVLSELREQFRPEFLNRVDEIIIFHALTKEEIRQIVDLQ
                     LQRVMKRLAEQEITLNITDEVRDYLAENGFDPKYGARPLKRLVQTAILDKVALALLKD
                     EKIRNVTISVQNTEIVINTERTSHPIEIPA"
     gene            10860..10934
                     /locus_tag="VGE59_00985"
     tRNA            10860..10934
                     /locus_tag="VGE59_00985"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10892..10894,aa:Val,seq:gac)
     gene            11053..11832
                     /locus_tag="VGE59_00990"
     CDS             11053..11832
                     /locus_tag="VGE59_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074262.1"
                     /translation="MKNPKIQLGIMLGGIISLLLVTVMLLSASPSSPKGSTPKPVVHQ
                     AAAQQGDSWGIVILIVAAVLMGLAVMRLVWAVINAPPPKKEDVVKYETQKEEYQRKWE
                     EYRRLRDEYETQRKKNHGLPPKDLPKPTQPDIPTPPSTAEKLKPVAVIVVTAIILYLY
                     HVCSSTVEMPFWTTTGASGVLVIAIFVLYISGLYPKHEDKEDTGGGDYPRYAWGRGWY
                     YDRSGLTGDRDDGSILGGMMGDGHDLDGDGEDDFDFDGDND"
     gene            complement(11928..12299)
                     /locus_tag="VGE59_00995"
     CDS             complement(11928..12299)
                     /locus_tag="VGE59_00995"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY1074263.1"
                     /translation="MNKRSGKYVALVGIVAILAGITAVFLRTEISADIQFGGQSGVKV
                     LVNDMVNIPVEEATVTLQTQNGTVQIVKTPDATGFYVSAARPGTYTLTVSAPGYISDT
                     KTIELSQGEHEEVRFFLSAEL"
     gene            complement(12296..13330)
                     /locus_tag="VGE59_01000"
     CDS             complement(12296..13330)
                     /locus_tag="VGE59_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY1074264.1"
                     /translation="MDKPNDVRITIAPQAIISVGLTLLGLWFLYLVRDILFLFLIVVA
                     VVVTFSPIIRSWERYMPRTLAIVTLYVLLFLGVVLVTALLLPSLINQFSDFLVYLDNR
                     FLHGGQADGYIQDIRHSLSLFLQGQSTEGLTRILTQFQGSLGAVYNTTLGFLGGVVAV
                     LTVFVTSFYLLQEEKNLQNFLAGFLPTSQHKRIVSMVNRISAKMSNWLRGQLLLMVIV
                     GILTGTGMAILGVPYPLVLGLFNGLMEAVPYVGPFIGAVPGIIIAFTVLGPVKGFIAL
                     AIYLFIQQIENQFLAPKIMSRALGLSPVIIIFALLTGGKLLGLVGVIIAIPIVAALSA
                     FYEEWRKESE"
     gene            complement(13529..13604)
                     /locus_tag="VGE59_01005"
     tRNA            complement(13529..13604)
                     /locus_tag="VGE59_01005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13569..13571),aa:Arg,seq:cct)
     gene            complement(13631..14416)
                     /locus_tag="VGE59_01010"
     CDS             complement(13631..14416)
                     /locus_tag="VGE59_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY1074265.1"
                     /translation="MRILFIGDIVGRPGRNAVKALLPALKQDYKFDLTLANGENMAAG
                     IGMTYEKYQEMKEVGIDYFTGGNHTLAKKEFWPYLDDPNVSVLRPANYEGALPGRGDV
                     VLDVAGKKVHIINLVGRAFMKGDIGNYLDYADTILEAAEADIRIVDFHAEATSEKIIL
                     ADYLDGKVTAVLGTHTHVPTADTRILPKGTAFQTDIGMTGPLNSSLGAKIEPYYQFAR
                     FNTPALYKVASGPVTFNAVYLEIDDETNKVTKIERIQRQVDAS"
     gene            complement(14511..>15923)
                     /gene="rny"
                     /locus_tag="VGE59_01015"
     CDS             complement(14511..>15923)
                     /gene="rny"
                     /locus_tag="VGE59_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY1074266.1"
                     /translation="LQKTSEDLDTKHKTLLLEAKDEALKIKEKAVKEYEQKEKYLKEL
                     EEKIRTRESSLDKRAEQLDSDKQRLTSQEQVLRTTQNELDELKKQQVGQLEKVAKLTM
                     EKAREQLLASAEKEYAPDILNQLKKQREALKEDADKEARKIIATAINRLATDQIAEST
                     VSAVALPNEEMKGRIIGKEGRNVQAFEKATGVDVIIDETPDSVGLTSFDPVRRAVAKM
                     ALEMLVSDGRIHPTRIEETVTKAKSEINNQIKEAGEAAVEEVGLPALPTDLVKILGRL
                     KFRTSYGQNVLKHSVEVAHIATLLGQEIGANIDVLKRAGLLHDIGKAMSQETPGGHLQ
                     LSVDICRKYGLSDEVIHAVEAHHEDIEAETVEAIIIKAADAMSGARPGARRESLESYV
                     KRLTELENIANSFKGVDKSFAIQAGREIRIIVKPEDVDDLGAFKMARDIAKKIEDNLQ
                     YPGTIRVNVIRETRASEVAK"
BASE COUNT         4616 a         3805 c         3389 g         4113 t
ORIGIN      
        1 actttgcctt ctaccttcgt ccaagattct acatcttcac caaaaacgat ccagttcatc
       61 gcaccatcgt cttgccggaa accaagcagg aagaacttgt cattccagcg actattaagt
      121 tagaacaggt tcgacaacaa ctatccttcg acgaacaaat gcgcattgac cgactcttag
      181 aaagcgactc gctaacgctc tcatttgaac tagccaagaa aggaggcatc gatgtctcgc
      241 ttgtcccaat caaagactaa aggttcagct cgaacgcaaa tcgggatcaa ggaagtaagg
      301 gataatattc tcattcttcc tgacaaccgc taccgcatga ttctcaccac ctcatcgtta
      361 aacttcgaac tccaaagcga agctgagcaa gatgccctca tggattcgtt ccagtcgttc
      421 ttaaactcaa ttaccacacc cctccaaatc cttattcgag tacgagaact ggatattgag
      481 cgctaccttg aagatttcca ggcttcccga gctaacgaaa ccgaagctat ttacaagaaa
      541 cagctagcta attacagcga gtttatacgt aagctcgtgg ccgggaacaa gatcctttct
      601 cgtcgattct acgttgtcat tccctacgag aaccgaggca taaccgactt tgccacgatt
      661 aaagaacagc tcatgcttga gcaggagatt attatcaaag gactggagaa gcttggtatg
      721 actgccaggc cgttgtctag tcttgagatt cttgatctct tctacagttt ctatcagcct
      781 gaacaagcca aaacccagcc attagctcaa gccttagtga gacaatccaa tgccttcccc
      841 catatctaaa ttgaaaacca aattaaataa gattcgcgac gaacgctggc agcgtaaaca
      901 gacgctcgag tttcatgtcg gcgaccaaga ccagcttgac ctgattactt attcagggct
      961 agaagaacag cgtaactatc tcgtcataga taaccgatac gttcgaacat tgtttgtatc
     1021 gggctatcca tacaccgctt ccagcggctg gcttaaccaa ctcattaact ttaactacaa
     1081 tgtggacatc agctaccaca ttgaaccaat cgaagcgacg ttggcgttac ctaaacttaa
     1141 tcggaagatt acggaactaa cttcgacaaa gcgtgccatg gaacgagctg gcaagattat
     1201 tggtgccgag ttaatggatc ctttggaatc ggccattgaa ttacgcgaca aaattcaacg
     1261 cggccaagaa aacctctttc aagtctcgat ctacactacg ctttcggctg actcattggt
     1321 cgagcttgag aaaaccacta agcttcttga gactgtctta gccactcgtt tgttctacac
     1381 caaatacgca acctttcagc aggtcgaagg gttacagtcc gtcttacctc gaggagataa
     1441 tctactcgct caaaagcgta acttagacag ctcctcaact gctctaacct tcccgtttat
     1501 ttcttctgaa ttagtccatg agtccggcat tctctacgga gtgaaccgct ccaatagttc
     1561 actcgttatc cttgatcgct tcgcattacc aaacgctaac agtattttgt ttgcgcaatc
     1621 tggagcgggt aaaagctata cggctaaagt tgagattctt cgtaacttga tgcgtggcac
     1681 gaaggtgatt gttatcgatc ctgaacgaga gtaccgccaa ttggcagaat ccgtgaaggg
     1741 tacctacgtc aaactgtctg ttcactccaa ggaaaagatc aacccgttcg atctttcgat
     1801 caatgctttt accgaagcgg acagctttgc cgatcacgtt caatctctta ccgagatcat
     1861 ttccctcatg gctgaaggtt tggatgcgga agaaaagtcc gttgttgacc aagctattct
     1921 caagacctac aaaaaggctg gctggtcatc taagaaatca cgtaaacgag actcagctaa
     1981 ctatcccgtg ctctatgatt tctatgccac actaaaatct ctcaagcaac ataagctttg
     2041 ccgtcggctt gagaagtaca cgactggctc actcgccgaa gtctttgagg aacaaaccaa
     2101 tattcaatta gataatcgtc ttgttgtttt cgataccaaa gacctaccgg aatctctcag
     2161 ggaaattatg catctggtcg tggcgcaatt cgtgcagaac caagttaaaa gcgagccatg
     2221 taaacggctc ctcgtgatcg acgaagcctg gctaatgctc cagcacgaag aaagtgcaag
     2281 atttattgct ggcctggttc gtcgcgctcg taaatattac ttaggcgtca gtatcatttc
     2341 ccaacaagct aacgactttc tccagaacgc gtacggtcga gctattgcct cgcaaagtgc
     2401 gctccgtatc ttgatgcgcc aagacacaac aacgattcgt actgtcgccg aagagttccg
     2461 tttgagcgaa tatgaacaga agtttctctt aacttgcgag cgcggtgagg ctttgatcgt
     2521 cgccgaccaa catcacgtcg tcgttaagat tactgcttcc gaagacgagc acccactcat
     2581 tacgactgat ccggctgaga attaccgacc gaagaaatga caccccaaac cattagcgct
     2641 attgctaccg ctttctcaat gaagaaagaa attaagtacg tctttattac ttttttagtc
     2701 atctgtctta tcccagtttt taccgtcatc atgattaccg aagttggctt ggagatcgtt
     2761 tccggcgcct tggttagtcg cgacccacaa tctgtccaag tagacattcg agatcctcga
     2821 acaggtgaag tggttgacca tattgaatct cttggtgttt ggccggtctc cgggccgatt
     2881 agccttgagt tcggtgagat cgacttgccc taccagccgt ttcataccgg catagatatt
     2941 gcgaccttta cccgtaccgt cggtgatcca gtcgtggctt ttatggcagg caaagttacc
     3001 tacgctgact ctgtctcatg gggctacggc aagcatgtca aaatagacca cgggcatttt
     3061 acgagttcgg tctacgctca cctggatagc ttgaatgtgg ctgtaggtga tgaagtagag
     3121 attgggcaag ttattggcac acgcggctca acaggatggt ctacggggcc acatctacat
     3181 tttgagatca gggtatttgg tatccccgtc aaccccagag tctttttaaa tggagatcct
     3241 tagagatacc ccttagcctt gcgctagcaa tgagaggaac ttctcttctg aaagtttcaa
     3301 tagatctgca tcacgtgcca atggactcgc tgtaatttca gtaccaaacc aagctagggg
     3361 cacgaaattt tccaattctt gcgctgagtc aaattctacc tctgccctca ttaatccttc
     3421 aaacttttca tgatagattt tgatcgttat tgatgggcta tcattaacaa tatagctatc
     3481 tcgaatgata acttgtttag caagtgattt taacaaggca aattcttctt ctgaaacatc
     3541 tatcttttct cgacttcgtt ctacttctga gacatttatt tttcgttcta gctcaaatct
     3601 ttcgcctttt cgctgaacac gtagctttat tccatcggca ttgtagagaa aataacgttc
     3661 atactcaatt ttttctctag cagaaagatc cggaagttct tttataaaga attttcgttc
     3721 aatttcattt ttcattttta cacctcatgt tagcccccgt atcattattg taccagaaat
     3781 aaggaatcca gatggataac agatcataat ttactgtaat ttcacccact tgacaactat
     3841 ctgtatacag atatattatc gttatataga taacgaggag gggtatgaat ctcgacttat
     3901 tccaacctga tagtgtgcag aaaacaggct catcagcaac tgaaaataag ttcggtagtc
     3961 aggaaataat ttcgcttaaa gaatgtaagc agtatgtcgg tgagtttcaa ttatccgatc
     4021 aacgtattat tgagattaga aataatatga ttggcattat cgaccaggta ataaacgcat
     4081 acctaaggaa cgttgggtaa gaacatggat agagattaca ccaaagcctt aatctattgc
     4141 cgagtctcct ctgagagaca ggttaaggaa ggtggcggct tagacagcca agaacaccga
     4201 tgtcgagctg atgccgcttc aattggcgtt gaagttgagg aagtttttcg tgaagaggga
     4261 atatccggta gcctatttga tcgtccagcg atgatgaacc tattgaaata cttggacgac
     4321 cactgggaaa caaaatatgt cgtaatcttt gatgacctta aacggtttgc aagagaagtt
     4381 gaagttcact ttaaattaaa aaccgagcta cgagctagag aggctaaact ccgctgcctt
     4441 aactaccaat tcgacgatag tgcagaaggt gaatttgtag aaacgatatt tgccgctcag
     4501 aaccagcttg agcgtaaaca aaaccggcga caggtatgtc aaaagatgaa agctcgagtt
     4561 gagcgtggat attggtgttt tcacccacca atcggatatg agttcaaaaa gaatcgcgaa
     4621 cacggcaaac tcatggtacc aattcatgct attactaaca tactctcggt agcgttagtt
     4681 gatttctcag agaacagact actagaacaa actgatgttc ttaactttct cattgctaat
     4741 aatctgcatg gcctactcgg tagagacaaa agtaggatta accatgatta tgttaaacga
     4801 attttaaccg agcctatata tgctgggtta attgattacc ctgaatggag tatttctaga
     4861 aggaaaggtc atcacaaagc cattatttca gaagcggtgt atgacaagat ccaaagcaag
     4921 ctaagaaagc cggaacgtaa accccgagat aacgacaata gagagtttcc actacgaaga
     4981 gttatagatt gcgctttttg taataaaaaa cttaccggta gcacaagccg aggtaaacgt
     5041 ctgtattact cgcactatac atgcaacaac aaggagtgtt tagccaatcc gaaaaacatt
     5101 accgccagta gagttgagga ggattatatt caattacttg gaaatattaa aattgaaaag
     5161 gaggcattag aaatggctaa agtaatggcc atgagaattt ggcagcaaaa gattaaggat
     5221 attaactccg gcaaagccga tagtcagact gaaattaagg cgatagagaa acaaattgat
     5281 gaatatatca accttatccc tgctaccagc tcagagagcc tacgaagccg ctatgaggct
     5341 aagatcgtag aacttgataa tcacatcgtc gagttgaaaa agggtcttga aaataaaaaa
     5401 gagcctaact ttgaagaggc gctcgactta acactaaaat ttcttggaac acctgctgaa
     5461 acgtggcaac tctcgggtaa agagctcaaa actatggtgc ataatatgat atttgaagag
     5521 aacccgaaat actccctaaa agatggtttt ggaacaccaa aattaagcct tccattcggt
     5581 ttgagtgact tatttggtgc gttaaataac aatatggtgg acctgagggg actcgaaccc
     5641 ctgtccagaa gggggcaacc gcaagcgcta cacgtttagt ctgtttgtgt tctttccaaa
     5701 gtgcttgcaa cagacaatca tacactttgg gcagtgtcgt ggcttaactg gcttacgaca
     5761 accacctacg ccagggcagt ccgagtggtt atgcctcgtt tatcgctcgg atcccacgat
     5821 aaggagacat gcttaggttt ttaggcgaaa gcaggggcaa aagcaggagc tgagaacatg
     5881 ttcttagcta cggcagttac ctttgaaaat gcgtttgcat ttgttaggcg cagtttaacg
     5941 aggtacgctc gacgtgctct tacggcctcc cggttcctcc tgtcgaaacc aatgcaggcc
     6001 catatattta cctgttaagg atggatctac actaccagaa atacacagaa aagtcaatgc
     6061 tttcatcgac aaaataaata tttaatatta taatgttgct agtttctcac aggagaatga
     6121 aaatggaact caccattgtt cccggtggca aaaaggccaa gaaacaggat cctttcattg
     6181 agctgtgcga tggagtcatc catagttcta cgagaatccc gcctcgcacc catgtggggc
     6241 ccgtcatcga tcgcaggcga agaaaaaagc tgccggagaa cgttgttgac atccgagatc
     6301 gctttaagcg ttaaacaatc ccgcggccac gaggccgtat cgcaccttct gggccgccct
     6361 tttctgggcg gctctcttca tgaatgaaca actgttgcag aagctgcaag aagaacttta
     6421 ggctgaatcg ttggattttt agcaatgagt ttttcaatcc ctgctcgatc caggagccct
     6481 gcttgtggac cgatatgcgc caaaaccgat gtcgagtttg catttcccca aagcattgcc
     6541 tcgggaattg ttttgccttc ggcaaatgca acggtaaatg cagtcgcaaa agaatcgcca
     6601 cagcccgttc tctcaatcac gggaccgtca aagatatcga gttgatacat ttctgatcca
     6661 ttatatacat aggatccctt aggaccgtcg gtaataacaa caacttcggg accaagcgct
     6721 ttcactctct ttaaaagctc actcatggta atgtgtgtat ccgccccaag tagcagaata
     6781 gcttcttctt tatttacaaa aaatagtgtg gccgccgcaa ttgatggctt aagaaatgca
     6841 agacctttct taagctgatg agttccagga ttatacgcca gcattgtgtg cttttcgcgc
     6901 actaaaccaa taactttgtc gtagattgct tcgaaccctt cgcccatact cgttaaataa
     6961 atccaacgtg gcgcttgcgc aatattccaa tgataggtac gaggaatgtg atttaaaagg
     7021 atcgtccgct ctccctgaaa gttaataacc accgaatagt tggttttctg atctttctca
     7081 atagtgagat attccgtact aaccccttcg gtatgaaaaa cattctgcac ttgctttccc
     7141 gtgtcatcat ttccgatagt acccataaaa gcggacgaga gattcagccg gcgagcgcca
     7201 acagctgcat tggcggcgtt cccgccaatg ataaaatctg ttcgtttggc agcaatttta
     7261 tcgccgtagt ttaaacacaa cttgcaatta tgcttatcaa tatcacacat gacgttcgca
     7321 tcatcaagct gcatgaacac gtcgagggtg gcatcaccaa tggcaacaaa gtcgtacatt
     7381 cccgctcctt atgtatgtct ctatactaat ttattttaga tcaaaacgca cgtgcctttt
     7441 ttgacatttt acagtattga caacgtgtaa agtttacttt acattatagt cactatgtcg
     7501 atgttacacc ttcgtcttaa acaactccgc cgcgaacgaa acatgagcca ggaagattta
     7561 gcccgcaaac ttggcgtgtc tcgtcaagcc ttaattgcca tcgagcaagg cgcttccctt
     7621 ccttcactac gtgtcattat ggcccttata gatgcgttag aagttacttt taatgatctt
     7681 tttgggcctg cctggagccc tttccgatca tctgattccg atacggaatc agaaagctca
     7741 cagctttcct tgtatcggta tcaggagagg ggaaaaacac tccctgtgac aatgcacgaa
     7801 gattcagcta cttttcatat ttatgctgaa cttgcaggag tacaagaaga agatttaaca
     7861 gttgatttaa gcacacagca cgttttaatt gttgcaatta aacgttcatg ccccccccct
     7921 tcaaacgatc tcctcatgca tatagaagaa attaactttg gtccgcttct tcgagttatc
     7981 gcgatgcctt ctcctattgc agccgatcag gcccgagcag agttcaaatc tggattactt
     8041 cacctgacgc ttccgaaact tcaacccgaa gtaagccgac gaatcacgtt taaaaaagaa
     8101 acaatccgac cagacaagat tcatcctaag gaggaatatg gatccgaata aatttaccac
     8161 caaagcgcag caagccctcg aatcggcctt tcaagcggct accgctcatg gccaggccgc
     8221 tgacgttacc cacttactga tttctctttt gcagcaaacc gaaggcacca ctcggactat
     8281 tttgaccatc cttgagaaac cgatcgatag gatattagaa gaaaccgaag attcccgtcg
     8341 gcacgctccc gcccaaagcc ccttttctaa tacaatgggt caggttatga tcactcaaga
     8401 tttgggtcgg gtcttaagcc aagccaaaca agaagccgag tcactcactg atgagttcat
     8461 ttcgacagaa catttactca ttgccttaac ccaggtaaaa accgccgctc aaacaatttt
     8521 ggacaaatat gggatccaac gcgaagccat tctgcgtgct ttagcctcca tacgcggagg
     8581 agaacgtatt acagaccaga atccagaaga taagtaccag gcgctcgata agtacggccg
     8641 cgacttaacc aagcttgctc gtgaaaataa gctagaccca gtgattggcc gtgacgaaga
     8701 aatccgtcga gtcatgcaag tactctcacg acgtacaaaa aataaccccg tgctaattgg
     8761 tgaaccagga gttgggaaga ccgctattgt cgaaggctta gcacaacgta ttgtaagcgg
     8821 tgacgttcct aatagcctca aaaataagcg tgtcatttct cttgatttag gtgcgatgat
     8881 cgccgggtca aagctacgag gtgaattcga agacagactg aaagctgtat taaatgaagt
     8941 aaaccgatct aacggccagg ttattctctt cattgacgaa cttcatacct taatcggagc
     9001 aggtgccgca gaaggtgccg tcgatgcggc caacttactt aaacctgcct tagcacgtgg
     9061 tgaactacat gccattggcg caacaaccct taaagagtac cagaaacaca tcgaaaaaga
     9121 cgccgctctg gaacgtcgat tccaacccgt ctatgtcaaa gaaccaaatg tggaagacac
     9181 aacggccatt cttcgtggtc tcaaagaaaa atacgagatt caccatggcg tgcgcattaa
     9241 agacagtgct atcgtagcgg ccgcaacatt agctaatcgc tatattgcag atcgattcct
     9301 accagataaa gccgttgacc ttctcgacga ggcaacgagc gccctgcgcc ttgagatcga
     9361 ctccatgcca accgaaatcg accaaacaaa acgtcgtatc atgcggctag aaattgaaaa
     9421 agaagcttta aaaaacgaaa aagaagacac ggctcgcaaa cgccttcgcg agcttgaaaa
     9481 agaactcgcc gacctcaaag agacaatggt aaaactggaa ggccgttggc aacaagagaa
     9541 agaagcactc gaccgaattc actcagccaa tgaagaaatc gagcaagcca aagccgaagc
     9601 gagcctttat gagcgacgag gcgagtttag caaagtagcc gagatccaat acaaacgtat
     9661 tccagaacta gaaaaacagc tcaccgaagc ccaacagcga ctcgccgcca ttcctcctca
     9721 agaaagaatg ctccaggatg caattagtga agaacaaatt gcgacggtag tctcccgttg
     9781 gaccggtatt ccggttagca aaatgctaca aagcgaacaa cgtaagctct ttgagcttga
     9841 gcagcaactg gcagagcgcg taattggtca acgcgaagca ctcgaagcgg tgagtaatgc
     9901 cattcgtcgc tcacgtaccg gtatcagcga tcccaaccgg cccattggtt cgtttatttt
     9961 ccttggccca actggcgtag gaaaaacaga aacagccaaa gcattagcca gcactatgtt
    10021 cgacagtgaa tcagccatta ttcgtgtcga tatgtctgaa tatcgcgaag cacactccgt
    10081 ctcgaagatg atcggatctc ctccgggata cgttggattt gaagaagggg gacaattaac
    10141 cgaacaggtc cgtcgcaaac cctattctgt tatcctgttt gatgaaatcg aaaaagctca
    10201 tccggaagta ttcaatatac tccttcagat cttagacgat gggcgcctca ccgactccaa
    10261 aggccgggtc gttaacttca ccaacaccct cataatcatg acatctaata ttggtagctc
    10321 ggcaattagc gactacaccg aacgaggcaa tacaaaacga attgggtata ttcctaataa
    10381 tgatcccgag gcgtcggaga aacaaatgga agagtcagtt ctctccgagc ttcgagaaca
    10441 attccgtcca gagtttctca accgagtcga tgagattatt atcttccatg ctctgacaaa
    10501 agaagaaatc cgacagattg tcgaccttca gttgcaacgt gttatgaaac ggctggccga
    10561 acaagagata actctcaaca tcacagacga agtacgtgat taccttgccg agaacgggtt
    10621 cgatccaaag tacggcgcac ggccactcaa acggctcgtc caaaccgcaa tcttggataa
    10681 agtggccctc gcccttctca aagatgaaaa gatcagaaac gttactatct ctgtccaaaa
    10741 cacggaaata gtaatcaaca cagaaagaac atcgcaccca atcgaaatac ccgcataatc
    10801 cacccatagt atctacccaa ggggcgagag atttgctata ataagcaggt accagccccg
    10861 ggcgattagc tcagtgatag agcgcttcgt tgacatcgaa gaggccactg gtttgatccc
    10921 agtatcgccc accatgaaag gcacacccga aagggtgtgc ctttttgtat gcaaataatc
    10981 taatatcacc attgacaaaa gtatagtttt atggtatggt gccagcaaga tcgctgacac
    11041 cttgagaaca tcatgaagaa cccaaagatc caactaggta tcatgctagg cggaatcatc
    11101 tccctattac tcgtaacggt gatgctactg tctgctagcc cctcctcccc aaagggtagt
    11161 acaccaaaac cagttgtcca tcaggcagcc gctcagcaag gagactcctg ggggatcgta
    11221 atactgatcg tcgccgctgt cctgatggga ctggcggtaa tgcgattagt atgggctgtt
    11281 ataaatgctc ctccgccaaa gaaagaagat gtggtaaagt acgaaaccca gaaggaggag
    11341 tatcagagga agtgggagga gtaccgacgc cttcgggatg agtatgagac ccaaaggaaa
    11401 aagaaccatg gtctccctcc caaggatctt ccgaaaccca ctcaaccgga tatcccgact
    11461 ccgccatcta cagcagaaaa actaaagccg gttgccgtaa tcgtggtcac ggccatcatc
    11521 ttgtaccttt accacgtgtg ctccagtacg gtagaaatgc cattctggac aacaacagga
    11581 gcctccggtg tcctcgttat cgccatcttc gtgctctata tttcgggtct ctatccgaaa
    11641 cacgaagaca aggaagatac gggcggcggc gactatccac gctacgcgtg gggtcgtgga
    11701 tggtactacg accgatccgg actcacggga gaccgtgacg acggcagcat ccttggagga
    11761 atgatgggcg atggccatga cctcgatggt gatggcgaag atgactttga tttcgatgga
    11821 gacaacgact agtcggtctc tccatcttga gactgagcgg gggctgtggc ccccgctctt
    11881 tactttctat aaatttagat actctcttct ctacctgtta tatcaaacta gagctcagca
    11941 cttaaaaaga atctgacttc ttcgtgttct ccctgtgaga gctcaattgt ttttgtatca
    12001 gagatatagc caggagctga aacggtaaga gtatacgtac ccggacgggc agctgaaaca
    12061 taaaagccag ttgcgtcagg agtcttaaca atctgcaccg taccgttttg agtctgaagg
    12121 gtaactgttg cttcttcaac aggaatattt accatgtcat tcacaagaac ctttaccccg
    12181 gactgcccac caaattggat gtctgcagag atctctgttc gaagaaatac ggcggtaata
    12241 ccagccaata tagcgacaat acccactaag gctacatatt tccctgatcg cttattcatt
    12301 cactctcctt acgccattct tcgtaaaaag cggagagagc ggccacaatc ggaatagcaa
    12361 taataacccc tacgagacca agtaatttac caccagtaag taaggcaaag ataataataa
    12421 cgggactcaa ccctaaggcg cggctcataa ttttaggagc aagaaactgg ttttcaatct
    12481 gctggataaa taagtaaatc gccaaagcaa taaatccttt gactggaccc aataccgtaa
    12541 aggcaatgat aatacccgga acggccccta tgaatggacc gacgtacggc acagcttcca
    12601 tgagcccgtt aaaaagaccg agcacgagtg gatacggcac gccaagaata gccataccag
    12661 ttcccgtaag gattcccaca attaccatca ataacaactg accacgtaac cagttgctca
    12721 ttttggcact aattcggtta accatactca caatacgttt atgctgtgaa gtcggtaaaa
    12781 acccagctag aaagttttga agattttttt cttcctgcaa gagataaaat gacgtaacaa
    12841 agactgtgag cacagccaca acaccgccca gaaaaccaag agtcgtgttg tataccgcac
    12901 ctagactccc ttggaactgc gtaaggatac gggtcaaacc ttctgtactc tgtccctgta
    12961 aaaagagaga aagagaatgt cgaatgtctt gaatataccc gtcggcttga ccaccgtgga
    13021 gaaaccgatt atccaaatag actaagaaat cgctaaactg gttgatgagc gatgggagca
    13081 aaagcgctgt aacaagcacc acaccaagaa aaaggagaac gtaaagcgtt acaatggcta
    13141 gagttcgagg catgtatctt tcccaagaac gaataattgg agagaaggta acaacaacgg
    13201 caaccacaat taaaaagaga aacaatatgt cacgcacaag atacaaaaac cataggccta
    13261 aaagggtaag gccaacactg ataatagcct gaggagcaat agtgatacga acgtcgttag
    13321 gtttatccat atattaagaa gtataagaaa atgggtagga gaatgcaatg gagcccccgg
    13381 caggattcaa ttcgtccgac ttcgtcggag tcagggcgaa ttgctccgga gaagtgtcag
    13441 ctccgctgat ttaatagcac ggatcgaacc tggttcgaat ccataatgat tttaagagtg
    13501 agtgttaact ttgctttatt acgtttagtg gagcccccgg caggattcga acctgcgacg
    13561 ccctccttag gacggaggcg ctctatcccc tgagctacga gggcttacgt tggttgtatt
    13621 ataccatgag ctaggaagcg tctacctggc gctgaattcg ttcaattttg gtgaccttat
    13681 ttgtttcgtc gtcaatttct aaatagacag cattaaaagt aaccggcccc gaagcaactt
    13741 tatataatgc aggggtatta aaccgagcaa attggtaata aggttcaatt ttcgcgccga
    13801 gacttgagtt aaggggaccc gtcataccaa tatcagtctg aaaggccgtt cctttcggta
    13861 aaatacgggt atctgccgta ggaacgtggg tatgggttcc taacacagcg gtgactttac
    13921 catcaagata atcagctaga ataatctttt cgctcgttgc ttcagcatga aagtcgacaa
    13981 tccgaatgtc agcttctgcg gcttccaaaa tcgtatcagc atagtccaag taattaccaa
    14041 tatctccctt cataaaggcc cgtccaacaa ggttaataat atgaactttc ttaccagcca
    14101 catcaagcac aacgtctccc cggcctggca aagcaccttc ataattagct gggcgaagca
    14161 ccgaaacatt tggatcgtct aagtaaggcc aaaattcttt ttttgcaaga gtgtgatttc
    14221 cgccggtaaa ataatcaatc ccgacttcct tcatttcctg atatttttcg taagtcatac
    14281 caataccagc agccatgttt tcaccgtttg cgagagtaag atcaaacttg tagtcttgtt
    14341 taagagccgg caataatgcc tttacggcgt tacgtccagg gcgtccaaca atgtcaccaa
    14401 taaataaaat tctcataata accttatttt acctgaatta tacttttact accagcatca
    14461 tagctcgcat ttttcttaaa acgccctctt tcgagggcgt tttaatgata ctattttgct
    14521 acttcacttg ctcgggtttc tcgaatcaca ttgaccctta tagtaccggg atattgcaag
    14581 ttatcttcaa ttttcttggc gatatcacgt gccattttaa aagcaccaag atcatccaca
    14641 tcctctggct tcacaattat acggatctcg cgtccagcct ggatagcaaa cgacttatca
    14701 acccctttga agctattggc gatattttct aattcagtta aacgctttac atatgactct
    14761 aagctctcac gacgagcgcc aggacgagca ccactcatcg catcagcagc tttaataata
    14821 attgcttcga cggtttccgc ttcaatatct tcatggtgcg cttcaacggc atgaataact
    14881 tcatcactta aaccatactt acggcagata tcaactgaaa gctggagatg tcctccggga
    14941 gtttcttggc tcattgcttt tccaatgtcg tggagcaatc cagctcgctt gagaacatca
    15001 atattcgcgc caatttcttg accaagaaga gtagcaatat gcgcgacttc cacagagtgc
    15061 ttgagcacat tttggccata ggaagttcgg aacttaagtc gtccgagaat ttttactaaa
    15121 tcggttggta acgcaggcaa cccaacttcc tcgacggccg cttctccagc ctctttaatc
    15181 tgattattaa tttccgattt ggccttagtc acggtttctt caatacgagt agggtgaatg
    15241 cggccatcgc tcacgagcat ttcaagagcc atttttgcaa ccgcacgccg cactgggtca
    15301 aaacttgtta agccaactga gtcgggagtt tcatcaataa taacgtcaac accggtcgct
    15361 ttttcgaacg cctgaacatt gcgtccttct ttgccaataa tccgaccttt catctcttcg
    15421 tttggtaatg ccacggctga aactgttgat tcagcaatct ggtcggtggc aaggcggtta
    15481 atggctgttg caataatttt acgcgcctct ttatccgcat cttccttaag agcttcccgt
    15541 tgttttttca actggttaag aatatcaggg gcatattctt tttcggccga ggcaagcagt
    15601 tgctcacgcg ctttctccat cgttaattta gccacttttt ctaattggcc aacctgttgt
    15661 tttttaagct catcaagctc attttgagtt gttcttagca cctgctcctg agacgtgagg
    15721 cgctgtttat cggaatcaag ttgctcggca cgcttatcaa gcgacgactc ccgggtacga
    15781 atcttttctt cgagttcctt gagatatttt tctttctgct cgtattcctt tacggccttt
    15841 tcctttatct ttaaggcctc atctttcgct tcaagcagga gtgtcttatg ctttgtgtct
    15901 aagtcttcgc tcgttttctg gag
//
LOCUS       DASWYB010000018         4792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_62327, whole genome shotgun sequence.
ACCESSION   DASWYB010000018 DASWYB010000000
VERSION     DASWYB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62327"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VGE59_01020"
     CDS             complement(<1..1061)
                     /locus_tag="VGE59_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY1074267.1"
                     /translation="MQRLQVVLDKAFREYGAALGIFILSRVLIFITFYAVISYGVGKP
                     SWTSWDAGHYLDIAVQGYTYGGANAKGNLIAFFPLYPLLIKGTSYLLSVPSLVAALLN
                     SFIFGLISTVLLYRYVADWKNKSVGLMAVFLFAFYPMTVFVSAPYTESLFFALTMGVF
                     LLLRRNQLFWASILIGLTSVTRMTGVLLVPLFLYVAFRNRVGWLKFLGYSFLMAYPFL
                     LFLFYQYVAFGTPLAFSLAQHIGWGKTIAGPWVGVITLLNNMPQHIMWQVDVLILAIM
                     CATLILSWRKVDRLLWFFGLEVILLTVSSNWILGTGRYMMLMLPFYVYWAEFLQKRET
                     LRQLVIAVCASWAMFNAAL"
     gene            complement(1186..1770)
                     /locus_tag="VGE59_01025"
     CDS             complement(1186..1770)
                     /locus_tag="VGE59_01025"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEY1074268.1"
                     /translation="MVEIHIRHAEASGEFGWGLTPTGIEQAKLAGDYVRKYFPADFQL
                     ALHSGSRRAVQTAEELGFPDLVWVKDERLREADWLGRPEPQDFLQWKEMSGRVANVCE
                     ELDTRFPTQNRLIVSHGGTMRMVRVYRERLGEDKFSTLFQSPYKYFTNCQLIIYTDED
                     PATGNITPDSLWVKSVCPWDESGRFGHDWIVVQL"
     gene            complement(1897..2343)
                     /locus_tag="VGE59_01030"
     CDS             complement(1897..2343)
                     /locus_tag="VGE59_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074269.1"
                     /translation="MQRNVGKNLITATLIAVGVAIAIPLYMSWWKGPEAITVAYSELL
                     THPSKYEGKLVAVEGWFIPVRTVTEIRPVTCLVGHTGFAPIFGVRNEPQQVTTYVFAP
                     NPDPTQFGPTRFISLDGWRCMRSTTAGGSRVWGLVGIDAAGGCYLH"
     gene            complement(2618..2836)
                     /locus_tag="VGE59_01035"
     CDS             complement(2618..2836)
                     /locus_tag="VGE59_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074270.1"
                     /translation="MKDISPVRVTLHVDREFREKVRKIVEPAATKEKLSEELKEQLVD
                     EVIEWTEILLESYRYHTSQEGQSSKSDE"
     gene            3259..3564
                     /locus_tag="VGE59_01040"
     CDS             3259..3564
                     /locus_tag="VGE59_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HEY1074271.1"
                     /translation="MSVRIVCVTKPSGNLANSHEAIEEYGWVNEKTGKSDITKRVKMV
                     EFLKGGGQAYVTNRQGIRAYCEVRTNQYGTEFLQTVTDGIYTDNLLSLDSCPVSQRQ"
     gene            3592..3840
                     /locus_tag="VGE59_01045"
     CDS             3592..3840
                     /locus_tag="VGE59_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074272.1"
                     /translation="MDDDIKDKLVEYLKVMTWYHSEKKEPTGLFATIGHIETTLSSLN
                     RNLENANNSSTKLSAALNWLTGVLAIAAIAQVVVTLLK"
     gene            complement(4096..4311)
                     /locus_tag="VGE59_01050"
     CDS             complement(4096..4311)
                     /locus_tag="VGE59_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1074273.1"
                     /translation="MDSKEDQLARKKLGKILKEAREKAGLTQAEVAAKAKVNANYYAI
                     VERGEGTYSFANLQRIMKVLHIKSLDL"
     gene            4382..>4792
                     /locus_tag="VGE59_01055"
     CDS             4382..>4792
                     /locus_tag="VGE59_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY1074274.1"
                     /translation="MLANSHQKNTVTSKLYNQTSFYSALANDMLKAKEEIIIYSPFVS
                     KHRTDTFLRLLDKLKDTNINVFIFTRPLREYAPGQRSEITEILGQYEETGATIYYLSG
                     YIHEKVAIIDREILWEGSLNILSQRSCREMMRRIT"
BASE COUNT         1499 a         1172 c          953 g         1168 t
ORIGIN      
        1 aacagggccg cattaaacat tgcccagctg gcacagaccg caatcacaag ttggcgcagt
       61 gtctcccgct tttgcaaaaa ctcggcccag taaacgtaaa acggcaacat aagcatcatg
      121 tatctccctg taccaaggat ccaattggaa ctcacggtaa gcaaaataac ctctaaacca
      181 aagaaccata acaacctgtc aaccttacgc cagcttaata tgagcgttgc acacataata
      241 gcaagtatca gaacatccac ctgccacata atatgctggg gcatattgtt aagtaatgtt
      301 atgactccaa cccacggccc cgcgatagtt ttcccccatc ctatgtgctg agctaacgaa
      361 aaggccaaag gcgtcccaaa ggcaacgtac tgataaaaca aaaaaagtaa aaatggatag
      421 gccattaaaa agctataccc caagaacttt aaccagccta cccgattacg aaatgcaaca
      481 taaagaaaaa gaggcactaa taatactccc gtcatacgag taacagacgt cagaccaatc
      541 agaatagatg cccagaaaag ttgattccga cgtaagagta aaaagacacc catggttaaa
      601 gcgaaaaata aactctctgt gtatggcgct gatacaaata ccgtcatggg ataaaaggca
      661 aataaaaata ctgccattag tccaactgat ttgttcttcc aatcagccac gtaccgatac
      721 aacagcaccg tgctaatcaa gccaaaaata aaagaattta agagtgcggc aacaagcgag
      781 ggcacagaaa gaagataaga tgtacctttt ataagaagcg gataaagcgg aaaaaatgct
      841 atgagattac ccttcgcgtt tgcaccgcca taggtatagc cttgaaccgc aatatccaag
      901 tagtgtcccg cgtcccaact ggtccaactc ggttttccca ctccataact gatcaccgca
      961 taaaaggtaa taaaaataag cactcgtgag agtataaaaa tgcccagtgc cgcaccatac
     1021 tctcgaaatg ccttatctaa aaccacctga agtcgctgca tgctattaca ataacatact
     1081 taaaccaaaa tcacgccgat aaagttaccg tttcttatag ttcttcaagg ttattccaaa
     1141 aaaggttttt agaaccaata caaagagatc cccatattac tcacattaga gttgtactac
     1201 aatccaatca tggccaaacc gtccggattc atcccagggg cacacagatt tcacccataa
     1261 gctgtcaggc gttatgttac ctgtcgcagg atcttcgtct gtataaataa taagctgaca
     1321 atttgtaaaa tatttgtatg gagattgaaa aagcgttgag aatttatctt cccctaacct
     1381 ctcacgatac actcttacca tccgcatagt cccgccatgc gaaacaatta agcggttctg
     1441 ggtaggaaaa cgagtatcta actcttcaca tacgtttgcc acacgcccag acatttcttt
     1501 ccactgcaag aaatcttgcg gttcgggtct tcctaaccaa tcagcctccc ttaaccgctc
     1561 atctttcacc catactaagt caggaaagcc aagctcctct gccgtttgaa ctgctcgacg
     1621 agaaccagag tgcaatgcta actgaaaatc cgcgggaaaa tatttacgta cataatctcc
     1681 cgcaagtttc gcctgttcga ttcccgtagg ggttaatccc caaccaaact ctccactcgc
     1741 ctccgcatga cgaatatgta tctctaccac ggaaccctct tttcttgtat tagcgcaacc
     1801 tcagactacc atacacaaag gaattattcg ataaggagta aagcccgcac agacggttaa
     1861 gtctacgcgg gccaaaagtt agatgatcct cgagcattaa tggagataac agccaccggc
     1921 tgcgtcgata cccacgagtc cccacacccg acttcctccc gccgtcgttg acctcatgca
     1981 ccgccaaccg tcgagcgaaa taaagcgggt aggaccgaac tgcgtagggt ccggattcgg
     2041 ggcaaagacg tacgtcgtaa cctgttgtgg ttcgttgcgt accccaaaaa tgggagcgaa
     2101 tcccgtgtgc cccaccaagc acgtaacggg acgaatttcg gtcaccgtcc gcacgggaat
     2161 gaaccagcct tcaactgcta ccaacttgcc ctcatacttt gacgggtgag tcagaagttc
     2221 ggagtaggct accgtaattg cttcaggtcc cttccaccag ctcatatata gcgggatggc
     2281 gatcgcgacc ccgaccgcta tcaaagtcgc cgttatcagg tttttcccta cgtttcgttg
     2341 cattgtgtcc ccctccacac agtgatgcat cttagcttag cagaaaaata tactccggtc
     2401 aagctacaaa ataaatagcg cccccgctct caggagcggg agcaacgaga agcagaacac
     2461 cacgcacctc ttatcaactc aaatcaacgc agactaaaaa cagccaccta tcggtgacta
     2521 tctttagtct ggtggacctc tcagtcgatc agtggatcca aatctataaa gaagcgcaac
     2581 aattgcgtga catctttgat gaagtcgaag agaccgacta ctcgtccgat ttcgacgact
     2641 gaccttcctg cgatgtgtga tacctgtaag actcaagcag gatctctgtc cactcaatca
     2701 cttcgtccac gagctgctct ttcagctctt ccgagagttt ctctttcgtt gccgcaggtt
     2761 caacgatctt tcgcaccttc tctcggaact cgcgatccac atggagagta actcgaactg
     2821 gtgagatgtc tttcatttat taaggctaac acgaattaca agcgtatttc ttgtattgta
     2881 atacgaaaac ggaaggcaaa atcaagcagt aaactgtagc tcacccataa atgtgtatca
     2941 tttactaaaa tgctaaccta gtgtaaaatc actttaaggg cacatacgcg tatcgcgtcg
     3001 aagatattat ttcagtacca cagaacaaac gacgtgaccg ttcccgcccg tcgttttttt
     3061 tattagttat acaaaaggag gacatttgta taagcagtac taaaacaaga aaccatgcct
     3121 gtggcgcaga catggtcctt ggtaatactt tccgcctctg aggtggaaat caacggtaag
     3181 gaaattggtg cccctccgct tttgatattc atctcatgac ctagttttaa aatcaagcac
     3241 caaggaaaag gtataatcat gtcagtacgt atagtatgtg taactaaacc cagtggtaac
     3301 ctcgcaaatt cccatgaggc aatagaggaa tacggatggg tgaatgaaaa aacaggcaag
     3361 agcgatataa ccaaacgtgt aaagatggtc gaattcctta agggaggggg acaggcatac
     3421 gtaacaaata gacaaggaat tcgagcatat tgtgaagtcc gcacaaacca atatgggaca
     3481 gagtttcttc aaactgtaac agacggaata tacacggata atcttctttc acttgattct
     3541 tgtcctgtct cacagcgcca gtaaaagcag aaataacaat aaaggagcat catggatgat
     3601 gatattaaag ataagcttgt cgaataccta aaagtcatga cttggtatca ttcagagaag
     3661 aaagaaccca ctggattatt tgcaactatt gggcatatcg agaccaccct atcatcgctg
     3721 aatagaaacc tagaaaacgc gaataattca tcgacaaagc tctcagcagc acttaattgg
     3781 cttaccggcg tattagcaat agcagcgatt gcccaagtgg tagtaacgct gttaaagtaa
     3841 gggcctagca atcttcggac tcagaatacc ggatcatatt caggttcgtg gttccaaaaa
     3901 cacaaatgta ccaccagaca ataagctggt ggtacattcg ttaactggag ctgtcaactg
     3961 ccgtttccgg cgtgaaaact tggtggacct agaccaaaaa acttggaaca ccttctttga
     4021 ctacgtaata gagtgggcgc ctaaattaag gcaaatatac gggaaacaac agagcgacaa
     4081 gggtcggtct gattcttata ggtctaagga tttgatatga agaactttca ttattctttg
     4141 cagattagcg aaggagtagg ttccttctcc acgttcaaca atcgcataat aatttgcgtt
     4201 cactttagcc ttggcggcaa cttcagcttg tgtcagacca gccttttcac gagcttcttt
     4261 caagattttg ccaagtttct tacgagcgag ttgatcttct ttgctatcca tataccgaca
     4321 ttataccaat tcgctactca gaattacaga tcaattaagg taaaatggtc taaggagcaa
     4381 tatgctagct aatagtcacc aaaagaatac agtcacaagt aaactctata atcaaacttc
     4441 cttctactcc gccttagcca atgacatgtt aaaggcaaaa gaagagatta ttatttatag
     4501 cccgtttgtc tctaagcacc gtaccgatac gtttcttcgc ctcttggaca aacttaaaga
     4561 caccaatatc aatgtcttca tctttacccg tccgcttcgt gagtatgcgc caggtcaacg
     4621 tagcgaaatc acagagatcc tcggccagta tgaagagacg ggtgcaacca tttattacct
     4681 tagcggttat atccatgaaa aagtcgccat tattgaccgg gaaatacttt gggaagggag
     4741 cttaaatatc ctttcgcaac ggtcttgcag ggagatgatg cgccgtataa cc
//
LOCUS       DASWYB010000019         4817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_375434, whole genome shotgun sequence.
ACCESSION   DASWYB010000019 DASWYB010000000
VERSION     DASWYB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375434"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VGE59_01060"
     CDS             complement(<1..361)
                     /locus_tag="VGE59_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074275.1"
                     /translation="MSDYEKVKELIPEACVSDFKDVFNTETSIVHGDWKHSQLMKVND
                     TYCIFDFGRSFYGPSLLDYGYYFKDEAGAGLEHFGTNKHLFLKAKLVTLIMQLEWFVR
                     CRDNYISYDYNKEISETQ"
     gene            complement(358..714)
                     /locus_tag="VGE59_01065"
     CDS             complement(358..714)
                     /locus_tag="VGE59_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074276.1"
                     /translation="MEIKITNAISLKVLTEWPLSKVELVSIGGKTYILKTVHSDFTNE
                     AQRQQLLSNLLTHPLTVPQVFDVLRTDTETKILMEYIEGTEPSGDDALHVIGLFMTRH
                     RIPIRIISHRITTSIF"
     gene            complement(750..2081)
                     /locus_tag="VGE59_01070"
     CDS             complement(750..2081)
                     /locus_tag="VGE59_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074277.1"
                     /translation="MDYTKLPTFETAQDYLSFQNQLNARMFEDGFHLLNQTECIPVLA
                     SSVILDKAATTDLIHSSKVIIGALQKVAKEYLKSTDLQNYVKIDPLELHLLTSTEQPY
                     GFGVTRLDSFLTDDGYKFVEINTDYPDGLTLFTQFVKTFDAFMEPYDSAYLKSDVNNL
                     ELLYSNLITLYQKNGGTHHNPTIACVGPLDRLWATEYNAHVEFLRSKGHKVFHVEPTE
                     LVFEDGELRYQNHKIDIVRRLCETRYFTKQPEACRAIFEAYKQKTAVFMNSFHDRLLG
                     VKGLFAVLTNSRYAHLFTSEEKEVIKRTIPVTKVVSTIDLHPDEITNICQNKDNYVLK
                     PSDLSEGEKIFVGCETDQHIWDEALSSALDQSDGRWIIQELIRIPKRESLIVENGELK
                     TESLYFDVCLHFFMDKNGDIATGTPFSRSSRSMILNVTKGGGANEVKLTYS"
     gene            2464..2763
                     /locus_tag="VGE59_01075"
     CDS             2464..2763
                     /locus_tag="VGE59_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074278.1"
                     /translation="MSLEVQEYLEEVTFIDEQRTRLAHIEPIPSLLLTHLSDVPAELL
                     QERLDWVPTQEEREQLRLRRMLTEYELEPEKFEIGDAICYRLTPGFQEFIDQQSR"
     gene            complement(3133..3798)
                     /locus_tag="VGE59_01080"
     CDS             complement(3133..3798)
                     /locus_tag="VGE59_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074279.1"
                     /translation="MKRQFWTDLLFAVQILCTLAFGGAQFVRLLATAQGVSTSWYVFW
                     EAFILMNFSLAVSAHRTQPSRVTAQTLVSYLCWMVMITADIAVMAWKQTGEWSLIDTM
                     TSTIATLGIVGTLVWARTRNIGISDPMAKGWLALFFKAVPQLMLAWNIAQRGGAGLTL
                     IAIITGHVTILTRLGQLYASIKEAGWDRNRTGSAFSEAGNEVSWVVVTIAWLVAHTRQ
                     LGG"
     gene            complement(3948..4022)
                     /locus_tag="VGE59_01085"
     tRNA            complement(3948..4022)
                     /locus_tag="VGE59_01085"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3987..3989),aa:Glu,seq:ttc)
     gene            complement(4108..>4817)
                     /locus_tag="VGE59_01090"
     CDS             complement(4108..>4817)
                     /locus_tag="VGE59_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074280.1"
                     /translation="VAIVPTATPTVKPTAIATPSSKVSIQQSTVAFNKTSAVADGVET
                     ITASVTLRTESGEVIKDIVPSIDGLRTTGDTSSPFALTEGGETWESHISSTVPGLITT
                     TITADTVTLATQDLTFIEPVAPADTTTDTNTGMSFTTMLLIGFLLLILLLLLLIFIWR
                     RLRKHDDEEQEGEYTDEAAYTDDQTAVQEDPNAYPVDPATQGNTVPPVIVAPVDQQEQ
                     QPPASGQPPAAPQTPQQ"
BASE COUNT         1286 a         1038 c         1111 g         1382 t
ORIGIN      
        1 attgagtctc ggagatctct ttgttgtaat catatgagat atagttatcc cggcaacgga
       61 caaaccattc taactgcata ataagagtaa cgagctttgc ttttagaaag aggtgtttat
      121 tcgtcccgaa gtgctccaat ccggcgcctg cttcatcttt gaaatagtaa ccataatcta
      181 aaagagacgg tccatagaat gatcgaccaa aatcgaaaat acagtatgtg tcgttaactt
      241 tcatcagctg agaatgtttc caatccccat gcacaatcga agtctcagta ttgaagacat
      301 ctttaaagtc gctcacacaa gcttcaggga ttagctcttt tactttttcg taatcactca
      361 aaaaatgctc gtagttatac gatgggaaat aatccgaatg ggtattctgt gtctggtcat
      421 gaaaaggccg ataacatgta gtgcatcatc tccagaaggt tccgtccctt caatgtattc
      481 cataagaatt ttggtttcag tatcagtacg taggacatca aatacttgag gaaccgtgag
      541 agggtgcgta agcaagttac taagaagctg ttgccgttgt gcttcattgg tgaaatcgga
      601 gtgaaccgtt tttaagatat aagtttttcc ccctattgaa accagttcca ctttagaaag
      661 tggccattca gttaaaactt taagtgatat agcatttgtt atttttattt ccatacatgt
      721 actatactaa aaacaatatt ttatatgaat tatgagtaag ttagtttgac ttcgttagct
      781 ccgccacctt tagtgacatt gagaatcata cttcgagagc ttcgactaaa tggagtgcct
      841 gttgcaatat cgccattttt atccataaag aagtgaagac agacgtcaaa gtagagtgat
      901 tctgtcttaa gttctccatt ttcaactatt aaggactcac gtttcgggat gcgaataagt
      961 tcttgaataa tccaacgtcc atcactttgg tctaaagcag aggaaagtgc ctcatcccaa
     1021 atatgttgat cagtttcaca gccaacaaat attttttcac cttcggataa atcacttggt
     1081 ttaaggacat aattatcttt gttctgacaa atatttgtta tttcatctgg atgaagatcg
     1141 atagtgctca caacttttgt tacggggata gtgcgtttaa tcacttcttt ttcttcactt
     1201 gtaaagagat gagcataacg agagttcgtt aagacggcga ataaaccttt tactcccagt
     1261 aaacgatcgt gaaagctatt cataaataca gcggtttttt gtttgtacgc ttcaaagatt
     1321 gctctgcaag cttctggctg tttggtaaag tatcttgttt cacagagacg acggacaata
     1381 tctattttgt ggttttgata tcgaagttcg ccatcctcaa acacaagttc agtgggttca
     1441 acatgaaaga ccttgtgtcc ctttgatcgc aaaaactcaa catgagcgtt atattccgtt
     1501 gcccataaac ggtcaagtgg cccaacgcat gcaatggtgg gattatgatg tgtaccaccg
     1561 ttcttttggt aaagggttat taagtttgag taaagcaact ctaagttatt gacatcactt
     1621 tttaggtaag cggaatcata gggttccata aatgcatcaa acgtttttac aaactgtgta
     1681 aaaagtgtaa ggccgtcggg atagtcagta ttaatttcaa caaatttata tccatcatca
     1741 gtcagaaaac tatccagacg cgtgacacca aatccatacg gttgctcagt actcgttaaa
     1801 agatgaagct caagaggatc aatttttaca tagttttgta aatcggtact ttttaaatat
     1861 tcttttgcaa ccttttgcaa agctccaata ataacctttg aagaatgtat taagtctgtt
     1921 gttgcggctt tatctaaaat aaccgaagag gctaaaacag gaatgcattc ggtttgattt
     1981 agtaagtgaa acccgtcctc aaacattcgc gcattgagtt gattttgaaa actaaggtag
     2041 tcttgggctg tttcaaatgt tggaagtttt gtgtagtcca tttgtataag tatattcccg
     2101 atggtgtaag aattcaagat ataagataac acgtgggttc gataaatctg gactatggca
     2161 caatgcgtat gttccctctg gcttattaga gtttttgctc tattacgcag taaagttttt
     2221 cgttacgaat aagatagctt catagcttac acacatgttg tattttcagt tggggaggtt
     2281 atcacatcgt gtctcgttac tcacgttcta cggccgaaga gacctggcga agcctgtctg
     2341 agcaagctcg aatagtagcc cagtttattg caacaggggg tggtcatgca ctagagctga
     2401 gcgagctaga agggatggcg caactacctc cagaaagggt gctagatatg caagcgtgcc
     2461 tcgatgagct tagaagttca ggagtattta gaagaggtta cctttataga tgaacaacgc
     2521 actaggttgg cacatatcga gccaattccc tctctgctac tgacgcatct ctcggacgtt
     2581 ccggccgaat tgcttcaaga acgtttagac tgggttccca cgcaggaaga aagagagcag
     2641 ttacggttac gccgtatgct tactgaatac gaattggagc cagagaagtt cgaaattggg
     2701 gatgctattt gctaccgtct tactcctggc ttccaagagt ttatagatca gcaaagtagg
     2761 taacagcggg catcctttta actttttctc cgaattgccg cctgcgggcg gcttttcttt
     2821 ttatataaaa cgaaacccgc cgcgtcagct accaaagcag ctaacgggcg ggtgtatttg
     2881 ctgcgaccag gggtctaatg gaggactctg gcttctccgg ccgggaacgt gatcatctgc
     2941 aatcccttag tgtagaaggt atacgttccg tcccggttca ggcggcctgc ggcctcctcg
     3001 cctgcccatg tggtccgatg ccacttgtag gtagagttgt gttcccccgt ggcataaatc
     3061 aacccacaca caatgccaca ggaggcgaga attggcaaca aggcccttat gagtcttttc
     3121 actttgagct cctcatccgc cgagctggcg ggtatgtgcg acgagccagg cgatcgtgac
     3181 aaccacccag ctaacttcgt ttccagcttc gctgaaagcc gaccctgttc ggttccgatc
     3241 ccacccagcc tctttgatgg acgcatacag ctgtccaagg cgggtgagaa tcgttacatg
     3301 gccagtaatg atcgcaatga gcgttaggcc tgctccacct cgctgagcaa tattccatgc
     3361 cagcataagt tgtggaactg ccttaaagaa cagcgcgagc cacccttttg ccatcgggtc
     3421 gcttatccct atgttacgtg ttctggccca tacgagtgtg ccaactatgc caagggtagc
     3481 aatggttgag gtcatggtgt cgatgagact ccactccccc gtctgcttcc acgccattac
     3541 agcgatatca gcagtaatca tcaccatcca gcataggtag cttacaagcg tttgagcggt
     3601 tacccgagat ggctgtgtgc ggtgtgcgga aactgccaag gaaaagttca tcaggatgaa
     3661 cgcttcccag aatacgtacc aggaagtact tactccctga gccgtggcta ataaccgtac
     3721 aaactgagca cccccgaaag cgagagtaca cagtatctgc accgcgaaca gcaagtccgt
     3781 ccagaactgc cgtttcactt ccgttcctta gagcgtcaga ttattaccaa gttaagctgc
     3841 aataatatta catatatacc acgagtagcc tgttttgtca atccaacgat aagttactaa
     3901 gtattaaaag tagaccaaaa aaccaccttt tcaggtggtc tcttgcttgg tagctcgtag
     3961 gggagtcgaa cccctctttc cgccgtgaaa gggcggcgtc ctagccgata gacgaacgag
     4021 ccatatttct aatataccta catattctat aataattacc gctttgaatc aagatccccg
     4081 agatgtctcg gggatcttga gttatctcta ttgctgcgga gtctggggag cggctggagg
     4141 ttgcccagat gccggtggtt gttgttcttg ttgatctaca ggcgccacaa tgacaggagg
     4201 cactgtattg ccttgcgttg caggatctac aggataggcg ttaggatctt cctgtacggc
     4261 tgtctggtca tcggtgtagg cggcctcgtc ggtgtactcg ccttcttgtt cttcgtcatc
     4321 gtgtttgcgt aagcggcgcc agataaagat gagaaggagg agtaagagga ttagaagcaa
     4381 gaatccaatc aagagcatgg tagtaaacga cataccggta ttcgtatctg ttgttgtgtc
     4441 agcaggtgct acaggttcga tgaacgtaag atcctgggtc gctaatgtca cggtgtcagc
     4501 ggtaatggtt gtggttataa ggccaggtac cgtagatgag atatgtgatt cccatgtctc
     4561 tccaccctcg gtgagagcaa atggacttga ggtgtcaccg gtagtgcgaa gtccatcaat
     4621 gcttggaaca atatctttaa tgacttcacc gctttcggta cggagcgtta cgctggctgt
     4681 aatggtttcg acaccgtcgg caaccgcact tgttttgtta aaggcgacgg ttgactgctg
     4741 aatactaacc tttgacgaag gagttgcaat cgctgttggt tttaccgttg gcgtcgctgt
     4801 tgggacaatg gcgacga
//
LOCUS       DASWYB010000020         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_472223, whole genome shotgun sequence.
ACCESSION   DASWYB010000020 DASWYB010000000
VERSION     DASWYB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472223"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..998
                     /locus_tag="VGE59_01095"
     CDS             246..998
                     /locus_tag="VGE59_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074281.1"
                     /translation="MTTQNDDLITALRGIGFSKNEAFVYLAALELGPSSIWQISKKSG
                     IKRPTCYVLLDELIWRGYAASTNDGKRTIYSVDSPKQLLRAVERRQERFVKSIAQLDA
                     LASKSPQKPTVRLYEGTNGVAEAYEESLSLPKGSEILIYGTAAVKTSYQEYITDYLKR
                     RVQKGIKVRAILPDTPENRAVEELNEKELRKTRYLPQEKFDPHTEINVFSDIITYIAH
                     SEASPFGTVIESPSLAALEKQRFELLWDIAQP"
     gene            complement(995..1729)
                     /locus_tag="VGE59_01100"
     CDS             complement(995..1729)
                     /locus_tag="VGE59_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074282.1"
                     /translation="MDLVQSLQEIGLQEREIKIYLALLELGESTVLPLSKQSGIKRTY
                     CYDILPLLQEKGLVSFIERNGRRHYSAEDPAKIEQLLKGRLERFIGILPDLRSIYGQS
                     PNKPRVRYYEGDDVGKEIVDELARSKSFDAISSPDHLNKYIGKSLLEELAVQMVKRGT
                     VSRELYTRGALPVTWPKLYKKPLQEVRYLPEGVTMQNDILIYGKKLVLISYEQQVHGL
                     VIEGSAIVDAHKQLFELLWKSTPTLG"
     gene            1982..2509
                     /locus_tag="VGE59_01105"
     CDS             1982..2509
                     /locus_tag="VGE59_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074283.1"
                     /translation="MFTWHPGNDLSNETIGEVLSTMVQPDQQEDIPEEVRMVENPEHW
                     FYRMLEIPGGSTLARHDPLHVLLEQGTHINAEAFVLGLSMGADEKAQQWHFSFYIWWV
                     THKTPQPFVFTQEAVDAYWLGVKTAEEMGLKNIHLVPLEEMHNQKINEVRKQMGINME
                     YLRHLYAQHKVLQTK"
     gene            2593..3201
                     /locus_tag="VGE59_01110"
     CDS             2593..3201
                     /locus_tag="VGE59_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1074284.1"
                     /translation="MMITQEPSLDRLTSFEKASWPEHEQASTSDLQQRLNAFPEGVFI
                     LSDNGTDVAQVTLSPKNIVLEDITSFERMRDLPVNRESTSAWVTNLAARTGEEFRGKG
                     YARTLLTEVVRWANRNGINMIAAGVTCAGYAKLLKSGEVTSIEDYLAQNRNPALKTFQ
                     SAAKANGCQCWHSTALANYWEIDEDSHGYGVLVVIDLRKENV"
BASE COUNT          987 a          885 c          762 g          823 t
ORIGIN      
        1 aggagatggg cactgagctg gatttacttg ttaattttct tgcctattgt gcgaatctct
       61 ttaagtagtt ctagacccgc tcgactagga tctctttcgc tccttcatcg tgacgagaca
      121 cattgtaacc caaaaagtct attttgtcaa gtattatgac catagagtaa agctaattta
      181 tttattatat ataaggtata aatattgaaa tatttggtac attttgtata atggtgtcaa
      241 taattatgac tacacaaaat gacgatttaa tcactgcatt gcgaggcatc ggattcagta
      301 aaaacgaagc ctttgtttat cttgcggctc ttgaattagg ccctagttca atttggcaaa
      361 tctcaaaaaa gtcgggcatt aagcgcccaa cctgttatgt tcttctagac gaactcatct
      421 ggcgtggcta tgctgccagc accaacgatg gcaagcgcac gatctactct gtagatagcc
      481 caaaacagct cctacgagcc gtagagcggc gtcaggagcg cttcgttaag tcgatagctc
      541 aattagacgc ccttgccagt aaatcgcccc aaaagccaac cgtacgcctc tacgaaggca
      601 caaatggcgt agcagaagcc tacgaagagt ccctgagctt gccaaaaggc agcgaaatcc
      661 tcatttacgg taccgcagca gtgaaaacct catatcaaga gtacattacc gactacctga
      721 aacgacgggt tcaaaaaggg ataaaagtgc gcgcaatcct ccctgatacc ccagaaaatc
      781 gagccgtaga ggagctaaac gaaaaagaat tacgcaaaac ccgttactta ccccaagaaa
      841 agtttgatcc gcataccgaa attaacgttt ttagcgatat catcacctat attgcccatt
      901 ccgaggcctc accttttggc acggtgatag aaagtccctc tctcgccgca ctcgagaagc
      961 aaagattcga attactctgg gatatcgccc aaccctaacc cagtgttggc gtagatttcc
     1021 agagaagctc aaagagctgt ttatgcgcat ccacgatagc ggacccctct ataacaagcc
     1081 cgtgaacctg ttgctcgtac gaaataagaa ctaatttttt accgtaaata aggatatcgt
     1141 tttgcatggt cacaccctca ggcaagtaac gcacttcctg cagtggtttt ttgtatagtt
     1201 taggccaggt cacaggtaaa gcaccgcggg tatacaattc tcgagaaacc gttccccgtt
     1261 ttaccatttg aaccgccaat tcctctagaa gcgactttcc aatgtattta ttgaggtgat
     1321 ctggagagct gatagcgtca aacgacttgg atcgagctaa ttcatctact atctctttac
     1381 ctacatcatc accttcgtag taccggacac gaggcttatt cggcgactga ccgtatatcg
     1441 aacgcaaatc gggcaaaata cctataaaac gttcgagccg tcccttgagt aattgctcta
     1501 ttttagccgg gtcttcagca gagtaatgcc tccggccatt tcgttcgata aaactaacca
     1561 accccttttc ctgtaacagg ggcaaaatat catagcaata ggtccgtttt attcctgact
     1621 gtttagaaag cggtaaaaca gttgactccc caagctccaa taacgctagg tagattttaa
     1681 tctcacgctc ttgtaggcca atttcctgca gcgattgaac taagtccata cactcctttt
     1741 cgtcactaat atacgacgtt tttatcattt tataaccata ttcacctatt ttcaagcttt
     1801 aaaggcatac tcgtattagt cgcaaacaat cgacaaaata gactttttgg gttacaatgt
     1861 gtctcgttac gctaagcaag caaccaaaag ccccttaaca acttcatcag ttcgacagtc
     1921 ataagtacgg cagcttgtga ctgacgacct ggttatcaac ctgcattacg gagaataaga
     1981 aatgttcact tggcaccctg gcaacgacct tagcaacgaa accatcggag aggttctctc
     2041 tacaatggtt cagcccgatc aacaagaaga catcccagaa gaagtacgca tggtcgagaa
     2101 tcccgaacac tggttctacc ggatgctgga gattccggga ggatcgaccc tcgcccgcca
     2161 cgatccgctg catgtactcc tagaacaggg tacacacatc aacgccgaag cattcgttct
     2221 tggcctgagc atgggagcag acgagaaagc gcagcagtgg catttcagct tctatatctg
     2281 gtgggtcacc cataagactc cccagccatt cgttttcacc caggaagccg tagacgctta
     2341 ttggctgggc gttaagacgg cagaggagat ggggctcaaa aacatccacc tcgttccact
     2401 ggaggagatg cacaaccaaa agatcaacga agttcgcaaa caaatgggaa tcaatatgga
     2461 gtacctccga cacctatacg cccaacacaa agtactccaa acaaagtaac ctccccgagg
     2521 ggtggttggc ctgtcactcg acgggccaaa tcttctcgga aggatcaata atttgagaga
     2581 acgttaggag acatgatgat tacgcaagaa ccaagcctag acagactgac ttccttcgag
     2641 aaggcgagct ggcccgagca cgaacaagcc agcacctctg acctgcaaca acgactcaac
     2701 gcttttccgg aaggcgtttt cattctttcg gacaacggta cagatgtcgc ccaggtaacc
     2761 ctgagtccga aaaacatcgt actcgaagac atcacttcgt tcgaaaggat gcgagacctg
     2821 cccgttaacc gggaaagcac ttcggcctgg gtaaccaacc tcgccgctcg aacgggcgaa
     2881 gagttccgtg gcaaaggata cgctcggaca cttcttaccg aagttgttcg ctgggcgaat
     2941 agaaacggaa taaacatgat tgccgcggga gtaacctgcg ctggatacgc aaagcttctt
     3001 aagtcgggtg aagtaacctc gattgaggat tacttggccc agaatcgcaa cccggcactc
     3061 aaaaccttcc aaagtgctgc caaagccaac ggatgtcagt gctggcattc caccgctctg
     3121 gcaaactact gggagatcga cgaagacagt catggttatg gcgtcctcgt cgtcatcgac
     3181 cttcgaaagg agaatgtcta accaccctga gaggtggtag ccctgacaga agtcggggct
     3241 gctcccctcg gaagacaaca agtccggaac cgaggtccgg cagaaaacta tgttcagcga
     3301 agactacgaa aaggggatca aagcgactct acccgtaacc attctcctcc tcccgatgct
     3361 gatcgtcctc atgatcaaca atcatgtcgc gcagcagcat cctatgatgg cagatctcgc
     3421 gatgggcttc gtgctagcgg catttgtagc tgaggtt
//
LOCUS       DASWYB010000021        12229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_881554, whole genome shotgun sequence.
ACCESSION   DASWYB010000021 DASWYB010000000
VERSION     DASWYB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881554"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..2197
                     /locus_tag="VGE59_01115"
     CDS             23..2197
                     /locus_tag="VGE59_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY1074285.1"
                     /translation="MRRFFALLAGIALAIPLNLLSVQAANLSFLEINFGEPTATLPLD
                     RFLKHKFTIKNTDSKAHTVSFSIRVKDTDQEIVGWPQPQMLYFNAGETLNVQTFLDEN
                     WGKIDKNEVGNHTRTVLWNFEESGQKRSESVTYKYSVIKDADVIGPTTFTGKVVDTKS
                     KPVSGATVSLTTGNFTKSTTSGTDGSFTFKNMPERSDWQIKATNGGQTGTAPAGVGGV
                     GGGAGQAQPGNPGAQCAPPPAPCNPPSGTQPAPNQPVSNQPGAMNFIETAHAASSAAS
                     AGYAFVEPGKTDYTITLKPASLESEYTVSKTVTSNIGYWKGDVDDAEQYVLVVQGMEN
                     WADPNLKSKPKLELYTIDGEKKWAYDMGWEGWGVDLSKDAKYAAFTTSNSTKTLGVID
                     AATGKALWTKTGKEFAGGVKVNNAAEGGGIDSKEVAISSTNKYLGVGHGGGNIFVADL
                     LTGKVAWSTDLKGQVRGIVFDKTDEYVYAGSGDGNAYKLKVSDGSIVWKADIGSWPFT
                     NGFKLSADGKYLGSASKVGEVTVIDTQTGKQLWQFDQQGNASILDFSPDGKYVFAGGG
                     GSGASTLYEAATGKKLWRLDRFSHEGKFSADGKYIMIGDVNIDFYDLNGNRVGGITPS
                     GEKSAASQGQFAFVSKDGTKVVYSRRDMAAEGNALLFATGKMTPVASADSANDTMTTL
                     ATTTESAKKGSSSLIVVLTVVLLAGAAGAIFILWKRKKAVTV"
     gene            2279..3073
                     /locus_tag="VGE59_01120"
     CDS             2279..3073
                     /locus_tag="VGE59_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1074286.1"
                     /translation="MKYAIEVQDLVKRYPNSEKNAVDGISFTVTPGEFFGFLGPNGAG
                     KSTTINMLVNLIKPTSGVVKIAGFDVQHQPDEVYKRVGFAMQEIGLDETATAREMMQL
                     HARLYHLPTENMDKKINDLLEMVELLPVADKYTSTYSGGMKRRFDLALSLIHEPKVLF
                     LDEPTVGLDPHARQLIWKHLKELNKKGMTILLTTHFMEEAEQLCDRIAIVDQGKMVTE
                     GTPKELMKEHKADSIEEVFLKVTGHSLGADEINQHAQDPFSRALPK"
     gene            3079..3846
                     /locus_tag="VGE59_01125"
     CDS             3079..3846
                     /locus_tag="VGE59_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1074287.1"
                     /translation="MNTFFADWFYLSIRNIKQIWRPLLAFVPNFFIPIFFFATNAVSF
                     QAVSKLPGFPTDSYLNFIAPTAIFTSVFFATSNIGIELVLDIASGYFKKLTIMPVNRL
                     AIILARLSEAAVLALIQGSVVLILVLLLGVKIATGFWGVVVMFLILMIFAMGWSCIGL
                     IAALRTQNPRMVQSLFILVFPFQYLTTSQMPIELLPRGFAMAVKLNPVTYILEATRSL
                     MLGSWSDAAINQGLMVAVATCVIMVTLTLLSYRKALK"
     gene            complement(3851..4087)
                     /locus_tag="VGE59_01130"
     CDS             complement(3851..4087)
                     /locus_tag="VGE59_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY1074288.1"
                     /translation="MALQRPRYPLPDDIQQRLTDQGLMDKYRERPEYQQNDYIGWITR
                     AKQDSTREKRLNQMIEELRGGTEYMKMKWHPKHK"
     gene            complement(4090..4506)
                     /locus_tag="VGE59_01135"
     CDS             complement(4090..4506)
                     /locus_tag="VGE59_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074289.1"
                     /translation="MQMSDSPLRTVAGIILGLLLIVGASLLIIRQSTHPIPLPSSSPS
                     TSPKISTFSGTLACLPHKDTKGPQTLECAYGLKMADAYYAIDTTFFPVNIASIPIGEK
                     VIITGDLTPIESMPNASLEKYDIQGTIRLSDIERIK"
     gene            complement(4533..5147)
                     /locus_tag="VGE59_01140"
     CDS             complement(4533..5147)
                     /locus_tag="VGE59_01140"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY1074290.1"
                     /translation="MRLNTIAFFGCADGHPGQDHFDAARAVATEIAKSGRTVLNGGGP
                     GVMLAATVGAKEGHGKTQVVYYKPEMATMFEGQAAVNHADIAFEESNYVERTKKLLEL
                     GDAYIIFNGGTGTISEFAMAWGVARLYFGHHKPLILYGDFWHDIMNTFKHTMLVRPEE
                     YQVFKIVNTPEQAMEHIHHYEHVVAAQHHDKEKCVGKECYLFLS"
     gene            complement(5267..5665)
                     /locus_tag="VGE59_01145"
     CDS             complement(5267..5665)
                     /locus_tag="VGE59_01145"
                     /inference="COORDINATES: protein motif:HMM:NF037921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074291.1"
                     /translation="MDSISLLPSPLNTIVIENKRGEYKDFGLDANDPYPLKGVTYPVD
                     YGYIPGYMGEDDAELDFFVGSDREGLSGSITVSRGKGNPDEHKFYIALTPEEREAILK
                     EFAPVLLQHTPIESKEQLTEAISDFKTTNA"
     gene            complement(5667..6038)
                     /locus_tag="VGE59_01150"
     CDS             complement(5667..6038)
                     /locus_tag="VGE59_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1074292.1"
                     /translation="MNELTGQCMCGAVTFKVTGEGTGIVSCHCKECQQLHGNYNPMWV
                     VNKEDFTVRGEDSLEWYNSSEEKSRGFCKKCGAAMFMRQETGPKMLISVGCIDDTVGM
                     ENIKNVFTQDAGTYYVMSEES"
     gene            complement(6065..6490)
                     /locus_tag="VGE59_01155"
     CDS             complement(6065..6490)
                     /locus_tag="VGE59_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY1074293.1"
                     /translation="MNDLTAHVSTEINAPIEKVWDALTKPELVKQYFFGVDLVTDWVE
                     GSDIIYKGEWEGVPFEEKGTVLKVDKPTQLVSNYWSPSYGLPDTIENRQVITYDLEEN
                     DGKTTVSISQEGSKTKEAKEHSEANWKLVLDGMKELLEK"
     gene            complement(6505..7986)
                     /locus_tag="VGE59_01160"
     CDS             complement(6505..7986)
                     /locus_tag="VGE59_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074294.1"
                     /translation="MKQPKVWIPLALLLVLVWFFRPFFHGIVMSFIVSPLQFVLIVLL
                     VIGAVTAISRLRDTVKVIKVSSDNYRFEASPGARRSLGLYLVLFFLVLFGLFIQNEVR
                     SYLTSRQITFEDRETLPTVDPIRLMPKSVAARYGQDSFQNPQEHLGDSQIVLMNGKLQ
                     RVFPRLPEGGLLYFFKKMSGFATVEVDTLDRKVSLEDQEFALSEGVGIFDNLHYRLPL
                     KRYFVNYTNEPIYLKDDSGKWVTVVPYITYKGFPFTVPQWGGVMVVHADGSMQDYTPQ
                     EAQALSYLKGNRIHPKELAVYYANSYAYKGGILNKWFLHKNETQVVSLPGDEAVIHTA
                     TNDGFKQIIVAEPYGSSYGIYKIFLIDATTGKREVISFSQNSQLTGPVAAADYIKKEF
                     PTFDWTAFSLAEPRPLVTNNKLYWLLSIIPNDSAGIANTVLLDTTTNNVTTVKTEAEL
                     KAFLAGKELPSTDQPAPVDTQQAIKQKIEAIENQLKELKALVQ"
     gene            8108..8845
                     /locus_tag="VGE59_01165"
     CDS             8108..8845
                     /locus_tag="VGE59_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074295.1"
                     /translation="MSSLLLYFTGARPMVLTVAIDGLNRSGKGTQLHLLKRFLQDRGL
                     PAVILRGDGSREGKGLSEGDPCDEWWQRFRSRLADAVTSMQRFACWNEAADRLAEEYL
                     HWRDSLMPQQVQRVNAPCGVILLDRSLLSRLMLAADERGSISLEGLYRTEYHGTPVTW
                     ETMMPDRMLVLVAQQDTLLARLDPDDPKYGFRSGLILEKYSLFQAVLDGLPQELQGVT
                     RHVDADQSIESVWRVCLEEAKCVIARK"
     gene            8910..9335
                     /locus_tag="VGE59_01170"
     CDS             8910..9335
                     /locus_tag="VGE59_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074296.1"
                     /translation="MINTFKVVVSAIIFNNQGEVLLGKRSLHEDVFPGLWGIPGGKIE
                     VEESGSNTVESALIREAKEEMGITIQPDHYIESSCRVKDGEAKLYLIYTALHVDGEPQ
                     ALEDTEAVQWFKIDELKEDELTPHTYHNILTAHRTFRPH"
     gene            complement(9332..10849)
                     /locus_tag="VGE59_01175"
     CDS             complement(9332..10849)
                     /locus_tag="VGE59_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074297.1"
                     /translation="MDNIDPSSTKTSHTELPHITLVETANTASGPVVLVGLGLHAKRI
                     YIHFLKKRHSLPKLIIELESNAAATQSYLDTHNIAAELFLVPDSERDNTELSVVTRDA
                     LKKAVADYGIRKAIISTEPKSHLAYASFFLEQDIDVLIDKPISAPVGASSDREAAHRI
                     YEDYQKLYQLAQKAPTAQCIVQSQRRYHAGYLKVRELVSSVIQQYGVPMTHLYVYHSD
                     GLWSMPNEYGERENHPYKYGYGKLMHSGYHFVDLFCWLTELNNNLPLKKPNELELFHQ
                     ICEPNDCLFQINQQDYARLFPANTLNPFFEKHNETDYQNFGEVDSYTQLQLRRDGKTV
                     LTGALHLLQNGFSRRSWQALPQDTYKSNGRVRHEQITLHVGPLMSIQIHSYESHEVAD
                     PNRITPSTEVGGLDHFEILVFRNVAMIGGEPFERILVTQDSRDNADYIGHNEEARQKC
                     LEDFMKSEKSKKPSESSIERHNATNWLLSQIHRASCQKRQGETPVITISLPEFSN"
     gene            10974..11822
                     /locus_tag="VGE59_01180"
     CDS             10974..11822
                     /locus_tag="VGE59_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074298.1"
                     /translation="MTPREPLYPNQVIPLVITALVCAFGVAIVAGEIFLLNTFVPQTA
                     SISLVIRWPDVLVGLTIYLKTAIDFAIFMGRLMDSFRGWKNRIAIEIGSALGNILGTV
                     LILLIWTLFKEVTLLMVAMIAIASLVLLRMAEEGLEHVKDSGNNATSLVTSAEWLERV
                     LQSINKIFAPLLRYIIPEMKVSGDKNKSWWGLFGLAFSVPFILGLDDFAGYVPLFNII
                     NVFGFATGVFLGHMILNIALFISPDSTIRIVKHPVISFIGSLAFIGLAVWGLYEGYHL
                     LATLWH"
     gene            complement(11888..>12229)
                     /locus_tag="VGE59_01185"
     CDS             complement(11888..>12229)
                     /locus_tag="VGE59_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1074299.1"
                     /translation="VKHMDFTLEGEQFGAMDSAYEHKFTFNEAISFMVHCETQEEIDY
                     YWDKLTAVPESEQCGWLKDKYGVSWQIVPNAMDRMFADGDQEKIARVTEAFLKMKKFD
                     LAKLEAAAEGK"
BASE COUNT         2951 a         2762 c         3110 g         3406 t
ORIGIN      
        1 tttttataga aagaggaggg acatgcgacg attttttgct ctacttgctg ggatagcctt
       61 ggcaattccc cttaaccttt tgtcggttca ggccgccaac ctatcgtttt tagaaataaa
      121 ctttggtgag cctacggcca cattacctct agaccgattt ttaaaacata aattcaccat
      181 taaaaacacg gacagtaaag cccatacggt tagttttagt atccgggtga aagatacgga
      241 tcaagaaatt gtggggtggc cacaacctca gatgctctat tttaatgccg gcgagacatt
      301 gaatgttcag accttcctcg atgaaaattg gggtaaaatc gataagaacg aagttggcaa
      361 tcatacgcgc acggtgcttt ggaattttga agagagtggg caaaagcgta gtgaatccgt
      421 gacgtacaaa tattcggtta ttaaagatgc agatgtgata gggccaacga cttttacggg
      481 gaaagtcgtt gatacaaaga gtaaaccggt atcgggcgca acggtctccc tcacaaccgg
      541 taattttacc aaatcgacaa cctcgggcac tgatggtagt tttaccttta agaatatgcc
      601 cgagcgttcc gactggcaaa taaaagcaac gaacggagga caaaccggaa ctgctccagc
      661 cggtgttggt ggtgtcggtg ggggagctgg acaagctcaa ccaggaaatc ctggcgctca
      721 atgtgcgcct ccgccagctc catgtaatcc gccaagtgga acgcagccag cgccaaatca
      781 gccagtttca aaccagccgg gagccatgaa ctttattgag actgcccatg cagcttcctc
      841 cgctgcttcg gccgggtacg cctttgttga gccaggcaaa acagattaca cgatcaccct
      901 gaagccagct tctttggaat ctgagtatac agtcagtaaa acggttacca gtaacatcgg
      961 ctactggaag ggagacgtgg atgatgccga gcagtacgtg cttgttgttc aaggcatgga
     1021 aaactgggct gaccctaacc tcaagagtaa acccaagctc gaactgtata ccattgatgg
     1081 cgaaaagaaa tgggcctatg acatgggctg ggaaggatgg ggtgtcgacc tctcgaaaga
     1141 tgccaagtat gctgctttta caacgtctaa cagtaccaag acactcggcg tgatcgatgc
     1201 tgccaccggc aaagctctgt ggacgaagac cggtaaagag tttgcaggtg gcgttaaggt
     1261 gaataatgca gccgaaggcg gtgggatcga cagtaaagaa gtcgctatct cgagtacgaa
     1321 caaatatctt ggcgttggac atgggggcgg taatatcttt gtcgcagatc tcttaactgg
     1381 gaaagttgct tggtctaccg atcttaaagg gcaagtccgt gggattgtgt tcgataaaac
     1441 tgacgaatat gtctatgctg gttcgggtga tggaaacgcc tataaactaa aagtctccga
     1501 cggctcgatt gtatggaaag ctgatatcgg ttcatggcca ttcaccaacg gctttaaact
     1561 ttccgctgat ggcaaatatc tgggctctgc ttccaaagtc ggcgaagtca cggttataga
     1621 tacccaaaca ggcaagcagc tttggcagtt tgaccaacag ggcaacgcct caatcctcga
     1681 tttctcgcct gacgggaagt atgtcttcgc cggtggaggt gggtcgggtg cttcgacgct
     1741 ttacgaagcc gcgacaggga aaaagctgtg gcgtttagat cgtttctcgc atgaaggtaa
     1801 attctcggct gatggcaagt acattatgat cggcgacgtg aatatcgact tctatgattt
     1861 aaacggcaac cgagttggag ggattacccc ctcgggagag aaatctgccg cgtcacaagg
     1921 tcagtttgca tttgtctcaa aagatggcac caaagtagtt tattctcgcc gtgatatggc
     1981 agccgagggg aatgcattat tgtttgcaac agggaaaatg actcccgtgg caagcgccga
     2041 tagtgccaac gatacgatga cgacactggc tactacgact gaatcagcta aaaaaggtag
     2101 ctcgtcgctg attgtggtgc taacagtcgt cttgcttgcg ggagcggctg gtgcgatctt
     2161 tattctctgg aagagaaaga aagccgtgac ggtctaggaa atctgtgtga tgctcgccct
     2221 gctctctgaa gcagggcgtt ttttagccaa gtcaacataa tctggtatac tcaaaataat
     2281 gaaatacgcc attgaagttc aagatcttgt gaagcgctat ccaaatagcg aaaaaaatgc
     2341 ggtcgatggg attagtttta ccgttacccc cggtgagttt tttggttttc tcgggccaaa
     2401 cggggccggt aagtcaacga ccattaatat gctcgtcaat ctgattaagc ctacaagcgg
     2461 cgtggtgaag atcgcgggct tcgatgtgca gcatcagccg gatgaagtgt acaagcgggt
     2521 tggatttgcg atgcaagaaa ttggtcttga tgaaaccgca acggcgcgcg aaatgatgca
     2581 gttgcacgcc cggctttatc atctgccaac cgaaaatatg gacaagaaaa ttaatgatct
     2641 cttagaaatg gtggaactac tccctgtggc cgacaagtac acctcgacct attcgggagg
     2701 gatgaaacgc cgtttcgatt tagcgctttc ccttatccac gagccgaaag ttctttttct
     2761 cgacgagcca accgtgggct tagacccaca tgcccgacaa ctgatttgga aacaccttaa
     2821 agaactcaat aaaaaaggga tgaccattct gctcacgacc cacttcatgg aagaagccga
     2881 gcagctctgc gatcgcattg cgattgtgga ccaaggcaag atggtgacag aggggacccc
     2941 caaagagctc atgaaggagc ataaagcaga ctccattgag gaagtcttct taaaagtcac
     3001 tggccactcc ctcggcgctg atgagattaa ccaacacgct caagacccgt tttcgcgggc
     3061 gttaccaaag taggaatcat gaacacattt tttgctgact ggttttatct ttcgattcgt
     3121 aatattaagc aaatttggcg accgttgctg gcgtttgttc ccaacttctt tattccgatt
     3181 ttcttttttg caaccaatgc ggtttctttc caggcggttt ccaaattacc tgggtttccg
     3241 actgattcct acttaaactt cattgcgcca accgctattt ttacctctgt tttttttgcg
     3301 acgagtaata ttggtattga gctcgtgctg gacatcgcgt ctggttactt taaaaagtta
     3361 acaattatgc ctgtgaatcg tttggcgatt attttggccc gtctcagcga agcagccgtg
     3421 ctggcactaa tccaagggag cgtggtgctg attcttgttc ttctactcgg tgtaaaaatc
     3481 gcaacaggct tttggggtgt tgttgtaatg tttctcatcc tgatgatctt tgcaatggga
     3541 tggtcgtgta ttgggttaat tgccgccctt cgaactcaaa atcctcgtat ggtgcagtct
     3601 ctctttattc tggttttccc gtttcaatac ctaacgacct cccagatgcc gattgagctt
     3661 ttgcctcgtg gttttgccat ggctgttaaa cttaacccgg taacatatat tctcgaagcc
     3721 acgcgctcgc tcatgctcgg ctcatggagc gatgcggcca ttaatcaggg gctgatggtt
     3781 gctgttgcaa cgtgtgtgat tatggttacc ctgactcttc tttcgtatcg gaaggcttta
     3841 aaatagcacc ctacttgtgc tttgggtgcc atttcatttt catatattca gtgccgcccc
     3901 ttagctcttc aatcatttga ttgaggcgtt tttcgcgagt gctgtcttgt ttcgcgcggg
     3961 ttatccatcc aatgtagtca ttttgctggt actcgggccg ttctcgatat ttatccatca
     4021 atccttggtc tgttagtctc tgctgaatat catcgggaag aggatagcga ggtcgttgga
     4081 gcgccatgac tattttatcc gttcaatatc cgaaaggcga atagtgcctt gaatgtcata
     4141 tttttctagg cttgcattgg gcattgattc aataggagtg aggtctcctg taattataac
     4201 tttttcgccg atcggaatgg aagcaatgtt aaccggaaag aaagtggtgt cgatggcgta
     4261 ataggcatca gccattttaa gaccatacgc acattcaagc gtttgtggtc cttttgtgtc
     4321 tttatgaggc agacaggcga gtgttcccga aaacgtacta atttttgggc tggtactggg
     4381 gctgcttgag ggcaggggga taggatgtgt tgactgacgg attataagga gactcgcacc
     4441 aacaatcaga agtaacccaa ggataattcc tgctaccgtg cgtaagggag agtcgctcat
     4501 ctgcatgggt atgccgtagg gaagaattta ttttacgaca agaacaagta gcactctttt
     4561 ccaacacact tttctttatc gtggtgttga gctgcaacaa cgtgttcgta atgatggatg
     4621 tgttccatgg cttgttctgg tgtatttacg atcttaaaaa cttggtattc ttcggggcga
     4681 actaacattg tgtgtttaaa ggtgttcata atgtcgtgcc aaaagtcgcc gtagaggatg
     4741 aggggtttgt gatggccaaa ataaagccga gctacgcccc acgccatggc gaattcagaa
     4801 atggtacccg tgccaccgtt aaagataata taggcatccc caagttcgag gagttttttt
     4861 gttctctcga catagtttga ttcttcaaaa gcgatgtcgg cgtggttgac ggcagcttgg
     4921 ccttcgaaca tagtggccat ctcgggcttg tagtacacga cttgtgtctt gccgtggcct
     4981 tctttagcgc cgacagtggc ggcaagcata acgcctggtc ctccgccgtt taaaaccgtc
     5041 cggccagatt ttgcgatttc agtcgcaacg gctcgggcgg catcaaagtg atcctgaccc
     5101 gggtgtccat cggcacaccc aaagaatgca atagtgttga gtctcatata catctacatt
     5161 acggcttccc ggccaacttg gcaaccagca tgtagcagtt tctacaaaag aaaaaccggc
     5221 agcggtctac cggtgaattt ctcgatgcgt cttagttgct tgtaggttat gcgtttgtag
     5281 ttttaaagtc gctaatggcc tcagttagct gctctttact ctcgatagga gtgtgttgca
     5341 gcagaacggg tgcaaattct tttaagatag cttctctttc ttcgggagtg agagcgatgt
     5401 agaatttatg ttcatcggga tttcctttgc cacgggacac cgtaattgat cctgatagcc
     5461 cttctctatc tgagccaaca aagaaatcta gttcggcgtc gtcctcgccc atgtagcccg
     5521 ggatgtaccc gtagtcaacg ggataggtaa ctcccttaag cgggtaaggg tcattggcgt
     5581 ccaggccaaa atctttatat tcgccgcgtt tgttttcgat aacaatcgtg ttgagtggag
     5641 agggcaataa agaaatacta tccatgttag ctttcttcgg acatgacgta gtatgtgcct
     5701 gcatcttggg taaagacatt tttgatattt tccatgccta cggtgtcatc aatgcaaccg
     5761 actgagatta acatttttgg cccggtttct tggcgcataa acatggcagc gccacacttt
     5821 ttacaaaaac cacgggactt ttcttcggag gagttatacc actctaagct atcctcacca
     5881 cgaacggtaa aatcttcttt attaacaacc cacatggggt tgtagttacc gtggagttgc
     5941 tggcactctt tgcagtgaca gcttacaata cccgtgcctt cacccgttac tttaaacgta
     6001 acggcaccgc acatacattg accagttagt tcattcatag gactcctcgt ctgctaattt
     6061 agtgttattt ttcgagtaac tctttcatcc catccaggac gagtttccag ttggcttcgg
     6121 aatgttcttt ggcttccttg gtcttactgc cttcttgaga aatggataca gtggttttcc
     6181 catcgttctc ttctaagtca taggtaataa cttgccggtt ctcgatggtg tcgggtagtc
     6241 catacgatgg tgaccagtag ttgcttacaa gttgtgttgg cttgtcgact tttaagacgg
     6301 ttcctttttc ttcaaatggg accccttccc actcgccttt atagataatg tcgctgcctt
     6361 caacccagtc ggtgacaaga tcaactccaa agaagtactg tttgacgagt tctggtttgg
     6421 taagcgcgtc ccatactttc tcgattggag cgttaatttc ggtgctgaca tgagctgtta
     6481 agtcattcat atgcatcctt tcttttactg cacgagtgct ttgagctctt tgagctggtt
     6541 ctcaattgct tcaatttttt gtttgatggc ttgctgcgta tccacgggag ctggctggtc
     6601 ggttgacgga agttcttttc cggcgagaaa agctttgagt tcggcctctg ttttcacggt
     6661 cgtaacgtta ttcgttgttg tgtcgagtaa tacggtgttc gcaatgcccg cagagtcgtt
     6721 cgggatgatc gagaggagcc aatagagttt gttatttgta accagaggac gtggttcagc
     6781 taagctaaag gctgtccagt caaacgtggg gaattctttt ttgatgtagt cggcggctgc
     6841 aacgggtccg gtgagttggc tattttgcga gaagcttatg acttcccgtt tcccggttgt
     6901 ggcatcgatg aggaaaattt tataaatgcc atatgaggaa ccatacggtt cggccacaat
     6961 tatttgtttg aacccgtcgt ttgtagcagt atggatgacg gcttcgtccc caggcaagga
     7021 aacgacttgc gtttcgtttt tgtgcaagaa ccacttgttg agaatcccac ctttgtaagc
     7081 gtaactattg gcatagtaga cggctaactc tttggggtga atacgattgc ctttgaggta
     7141 cgatagtgcc tgggcttctt ggggcgtata gtcttgcatc gagccgtccg cgtgaactac
     7201 catcacacct ccccattgcg ggacggtgaa aggaaatcct ttataggtaa tgtacggcac
     7261 cacagtaacc cacttcccgg aatcgtcctt gaggtaaatt ggttcattgg tgtaattaac
     7321 aaagtagcgc ttgagaggta gccggtagtg aaggttgtcg aagatgccaa ctccctcaga
     7381 tagtgcaaat tcttggtctt cgaggctcac ctttcgatcg agcgtatcaa cttcaaccgt
     7441 ggcaaatccg ctcatttttt taaagaagta aagcagtcct ccttcgggca agcggggaaa
     7501 aactcgttgg agtttgccgt tcataagaac gatttgcgaa tcgccgaggt gctcctgggg
     7561 gttttggaag ctatcttgcc cataccgggc tgcaacactt ttcggcatca ggcgaatagg
     7621 gtcaacggtc ggaagcgtct cacgatcttc aaaggtaatc tgacgggacg taaggtagga
     7681 acgaacttcg ttttgaataa aaagcccaaa cagaacgagg aagaacagga caagatagag
     7741 ccccaggctg cgtctagcgc cgggtgacgc ctcaaagcga tagttatcac tcgagacttt
     7801 aataactttt accgtatcgc ggagtcggga gatcgcggta acggccccga taaccaacag
     7861 gacaataagt acaaactgga gcgggcttac aataaacgac atcacgatgc catggaaaaa
     7921 tggtcggaaa aaccatacga gcacgaggag cagggcgagg gggatccaaa ctttgggttg
     7981 tttcataaag tatcctctta tctttgcctg attataccat gttgttacgt ccagagttat
     8041 tttaaatatg actatttttg caggcatcgt ccatctctac aatcagaata aagtatagta
     8101 tactgcagtg agtagcttgc ttctctactt tacgggggcc aggccgatgg ttctgacggt
     8161 tgcgattgat ggactcaaca ggagtggtaa aggaactcaa cttcatctcc tgaagcgatt
     8221 tcttcaagat cgaggactcc cagccgttat cctccggggt gacggctcac gtgaagggaa
     8281 ggggttaagt gaaggtgatc catgcgatga gtggtggcag cgattccgat cccggttggc
     8341 tgatgccgta acgtccatgc agcgctttgc ctgctggaac gaagcggctg accgccttgc
     8401 cgaagagtac ttacactggc gagactccct tatgccgcag caggtgcagc gagtcaatgc
     8461 tccatgtgga gttatcttgc tcgatcggtc gcttttgtcg cggttgatgc tggcggctga
     8521 cgaacggggg agcatatccc ttgaaggatt gtatcgaaca gagtaccacg ggacgccggt
     8581 aacgtgggag accatgatgc cggacaggat gctcgtcctc gtggcgcagc aagatacgct
     8641 gcttgcgagg ctcgaccccg acgacccgaa atacggcttc cgttcgggac tcatcctgga
     8701 gaaatacagt ctcttccagg cggtccttga cggattgccg caagagcttc agggagttac
     8761 tcggcacgtg gatgccgatc aaagcattga gagcgtgtgg cgcgtctgcc tggaggaggc
     8821 gaagtgcgtc attgctcgaa aatgagcgaa actgctatga tctgggagcg gggtgactcg
     8881 ctcccagtat ttatatccta tggagtacta tgattaacac atttaaagtt gttgtgagtg
     8941 ccattatttt taataaccaa ggcgaagttc tccttggtaa aagaagttta cacgaggatg
     9001 tttttcctgg tctctggggc atccctggtg gaaaaattga agtcgaagaa agtggctcaa
     9061 acacggtaga atcagccctt atacgtgaag cgaaagaaga aatgggtatt accatccagc
     9121 cagaccacta cattgagagc tcgtgtcggg taaaagacgg agaagccaag ctctatctta
     9181 tttatacggc tcttcatgtc gacggcgagc cacaggcact cgaggacacc gaggccgttc
     9241 agtggtttaa aatagacgaa ctcaaagaag acgagctaac ccctcacacc taccataata
     9301 ttttaactgc tcaccgaacg tttcggcctc actaattcga aaattcaggt aggctgatcg
     9361 taatgacagg tgtctcgccc tggcgttttt gacagctcgc gcggtgtatc tgcgacagga
     9421 gccagttggt ggcattgtgt cgttcaatgc ttgattcaga aggctttttg cttttttctg
     9481 atttcataaa atcttcaagg catttttgcc gtgcttcttc attgtgccca atataatcgg
     9541 cgttatcccg gctatcttgc gttacgagta ttcgttcgaa tggttctccg ccaatcatgg
     9601 cgacatttcg aaataccaaa atctcaaaat ggtcgagtcc acccacctcg gttgatggcg
     9661 ttatccggtt gggatcagcg acttcgtgtg attcgtagct gtgaatttga atactcatga
     9721 gtgggccaac atgaagggtt atctgttcgt ggcgcacccg cccgttgctt ttataggtgt
     9781 cctgcggtaa tgcttgccac gaccgccgag agaaaccgtt ttggagcaaa tggagcgcgc
     9841 cggtgaggac ggttttgcca tcacggcgga gttggagctg cgtgtaagag tcgacttcgc
     9901 caaagttttg gtaatcggtt tcgttgtgtt tctcaaaaaa cgggttgagt gtattggccg
     9961 gaaagagcct ggcatagtcc tgctgattga tctggaaaag acaatcattt ggttcgcata
    10021 tctgatggaa cagctctagc tcgttgggtt tctttaaggg taggttgtta ttgagttcgg
    10081 ttaaccaaca gaataagtct acgaagtggt atccgctatg catcagctta ccgtatccgt
    10141 atttgtacgg atggttttct cgttctccgt actcattggg catgctccac aacccatcgc
    10201 tatggtagac atataaatga gtcatcggga cgccatactg ctgaatcaca gacgagacca
    10261 gttctcgaac tttgaggtaa ccagcatggt accttctctg gctttgaacg atgcactgtg
    10321 cggtcggcgc tttctgggcg agctgataga gcttttgata atcctcatag atacggtggg
    10381 ccgcttctcg gtccgacgaa gctccgaccg gcgcgctgat gggtttgtcg attaaaacat
    10441 caatatcctg ctccaaaaag aaactggcgt aggcgagatg acttttgggt tcagtcgaaa
    10501 taatcgcttt acgaataccg tagtcggcga cggccttttt aagggcgtca cgcgtaacga
    10561 cggataattc ggtgttatcc ctttctgaat ccggaactaa gaaaagctcg gccgctatgt
    10621 tatgcgtgtc taagtagctt tgtgttgccg cggcgtttga ttctaattcg ataatcaatt
    10681 ttggtaatga gtgccgcttt tttaaaaagt gaatataaat ccgtttggca tgaagaccaa
    10741 ggccaacaag aacaaccggg cccgatgccg tattggctgt ttcgacgagt gtaatatgcg
    10801 gaagttcggt atgcgacgtc tttgttgaag aagggtctat attgtccatg ggcatattat
    10861 acgcttaaat tttaaaaaaa gcagggtatg cttgcttttt ttgttgcaaa cgaactcttt
    10921 ttgtatgtct ggctctactc atagagcgta aagctggtat agtgggaaac tgtatgacgc
    10981 cccgtgagcc actttacccc aaccaagtta ttcctcttgt tattaccgcg cttgtgtgtg
    11041 cttttggcgt agcaattgtg gcgggggaga tttttcttct caacaccttt gtgccgcaga
    11101 cggcatctat ttccctggtt atccgctggc cagatgtgtt agtcgggctt accatctact
    11161 taaaaactgc gattgatttt gccattttta tgggccggct catggactcg ttccggggct
    11221 ggaagaaccg gatcgcgatc gaaattggct cagccctggg taatatcctc ggtactgtcc
    11281 tcattctgct tatttggacg ctctttaaag aggtcacgct cttaatggtg gcgatgattg
    11341 ctatcgccag ccttgtcctg ttgcgcatgg ccgaagaagg gctagagcac gtcaaagata
    11401 gtggcaataa tgctaccagc cttgtaacga gtgccgagtg gctcgaacgc gtgttgcaat
    11461 caatcaataa gatctttgcc ccgctccttc gctacatcat ccccgaaatg aaagtttcgg
    11521 gagataagaa taaaagttgg tggggtttat tcggtctggc ttttagcgtg ccctttatct
    11581 taggtctcga cgactttgcc ggctatgtcc cgctctttaa tattattaac gtttttggct
    11641 ttgcgacggg cgtattttta gggcacatga ttttaaatat tgccctcttt atctcaccgg
    11701 attcgacaat ccgtatcgtg aaacatccgg tgatctcgtt tatcggtagc ctcgccttta
    11761 tcgggcttgc cgtgtggggt ctctacgaag gctaccactt gctcgcaacg ctctggcatt
    11821 aaaaacgttt aggatccaaa aagggttact gcaacgtaac ccttctcccc ctctgatagt
    11881 atgatggcta tttgccttcg gctgcggcct cgagtttggc gaggtcgaat tttttcattt
    11941 tgagaaaagc ttcggtgacc cgggcaatct tttcttggtc gccatctgca aacatcctgt
    12001 ccatggcgtt gggaacgatc tgccacgaca caccgtactt gtctttgagc cagccgcact
    12061 gctcagattc tggcacggca gttaacttgt cccagtagta atcaatctct tcttgcgtct
    12121 cgcagtgcac cataaacgaa atggcttcgt tgaaagtaaa cttgtgttca tacgcactgt
    12181 ccatggcgcc aaactgctcg ccttcgagcg taaagtccat gtgcttaac
//
LOCUS       DASWYB010000022         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_932606, whole genome shotgun sequence.
ACCESSION   DASWYB010000022 DASWYB010000000
VERSION     DASWYB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932606"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VGE59_01190"
     CDS             complement(<1..648)
                     /locus_tag="VGE59_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074300.1"
                     /translation="MKRHTPHKYLSMGLCLLLIGIIIGIKALNRQSVPFASTLDSPNS
                     DESLTSAYLQGKVTLTNGSPATGVSIYINNQGAGIHNDGTYAFHADIPSGETSVRFYN
                     PLTGERYRLKGSVESTFSYDPTENRNYNFVIELITNASPSPTPNPTPTPTPLPVIQSF
                     VAEKNSVRQGTSACISWVTNNPVSLQLAVSGATSNIQLSSDSGRKCFTPSQTTTYT"
     gene            complement(645..2141)
                     /locus_tag="VGE59_01195"
     CDS             complement(645..2141)
                     /locus_tag="VGE59_01195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY1074301.1"
                     /translation="MFVSLEGTVTYKESGVGLEGVTIELTQGTKKFTTTTNNKGYYKF
                     PTISGDEFSHLQATKPKFKPYNYDLDLVSGTNTHNFTMRFSNDNEVDGMTYEMDHGAK
                     KPLGGITVVAENVDSTDSDDKTGEYELWDQKFVKGTYKLWAEDQKGKLSDVYTITFTE
                     DTDQVITQDIEIKRKPDNRYVLRVAAFDRGWWVRDGTEPIEGVNVTLEGTLLDGKKQT
                     TTQTTDKKGIATFTDLKEGTYKATGQKVNYWDGKGTITLSGKPDIYGYDLEIFLLVTG
                     VTCEKHSQTSVKEFWFCGKEAIDLYTKRQDLWTLIDNKISQVRGSYPQAQYADMPQQV
                     VIDSISYNNAFFYKNTEKYNGTCPISPLDDDPGFPLTADTIVFTTGALLKESTLSTEH
                     TVTHEMGHNIDWRKGNCKDEYSDSPPFKDVYWLGETLYGDDYFYGLKDSNYGTNETGG
                     HPNDNYHEGFASTVHAYLDHIGEFPKMRTAIDQLKEILLKTASLTGAV"
     gene            2140..2472
                     /locus_tag="VGE59_01200"
     CDS             2140..2472
                     /locus_tag="VGE59_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074302.1"
                     /translation="MVGEGLGDGLGLGVAGDTLGLSVAAWTEDKVTTQNNKKEVRYRR
                     KVRQSRCVIMSILVVFSYYKDCLLVSQVSTSQASLIDKTFSLGYHIATLIRSLSFALC
                     ARRPVVTD"
     gene            2599..>3235
                     /locus_tag="VGE59_01205"
     CDS             2599..>3235
                     /locus_tag="VGE59_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074303.1"
                     /translation="MEENNSSTPHSNEDRRPNRRPQRPMSRAPRMNAPHGHSVGDGNA
                     SSAQKQSNTQDRFMRPSLSRHGARPAGGDRMQQANNRGFVAPSERTQDRSNGFPRSPQ
                     RTPAARNPRLADGMQPQVMRRPNTRRHAPDGQPASAGGNGGEQRLNRRRFSPPRGGDN
                     KYAMVTQITEDAKNSSTKQVVLSSASRKLRIIPLGGAGETGSKNMTVFEYGE"
BASE COUNT          787 a          710 c          800 g          938 t
ORIGIN      
        1 tgtatacgtc gttgtttgag aaggtgtaaa gcattttcgt cccgagtcac tcgagagttg
       61 aatgttgctt gtcgctccag aaactgcgag ttggagtgat acagggttat tggtgaccca
      121 gctaatacag gctgaagtac cttgccgtac agagttcttt tcggccacaa aagactgaat
      181 aacgggaaga ggagttggtg taggcgtagg attaggtgtt ggcgagggag atgcattggt
      241 aataagttca atcacaaagt tataattacg attctcggtc gggtcgtacg aaaaggttga
      301 ctctacgctg cctttgagtc ggtagcgctc acccgtgaga gggttgtaaa accgcacaga
      361 agtttctccg ctcggaatgt cggcatgaaa ggcgtatgtg ccgtcgttat gaatgcctgc
      421 accttggtta ttaatgtaaa tacttactcc cgttgccggt gatccatttg tgagagtgac
      481 ctttccttgt aaataagccg aggtgaggct ttcgtctgaa ttgggtgaat cgagtgttga
      541 tgcaaaagga acgctttgcc tattgagagc tttgatccca atgataatgc caatcaaaag
      601 gagacaaagg cccatactta agtatttgtg aggcgtgtgg cgtttcatac cgctcctgtg
      661 aggctcgccg ttttgagaag aatttctttg agctgatcaa tcgcggtacg catcttggga
      721 aattctccta tgtgatcaag gtaggcgtgg acggttgagg caaatccttc atgatagttg
      781 tcgttaggat gtcctcctgt ttcattggtt ccatagttac tgtccttaag gccatagaag
      841 taatcatctc catacagtgt ttcaccaagc caatacacat ctttgaaggg tgggctatct
      901 gaatattcgt ctttacaatt tcctttgcgc cagtcaatgt tatggcccat ttcatgggtc
      961 acggtatgtt ctgtgctgag agtgctctct tttaagagtg cgccggttgt aaagacaatt
     1021 gtgtcagcag tgagggggaa tccggggtca tcatctagag gagagatagg gcatgtccca
     1081 ttgtattttt cggtattctt atagaaaaag gcattattat acgatattga atcaatgaca
     1141 acttgttgcg gcatatcagc atattgagcc tgtggatagc taccacggac ttggctgatt
     1201 ttgttatcga ttaaagtcca gaggtcttgc cgcttcgtat acagatcgat ggcttctttg
     1261 ccgcagaacc aaaactcttt gactgaagtt tgagaatgtt tctcacaagt aacacctgtt
     1321 actaagagga aaatttcaag atcgtatccg tatatatctg gtttgcctga tagggtgata
     1381 gttcctttac cgtcccagta gttgactttt tggccggtag ctttgtaagt cccttctttt
     1441 aaatctgtga aggtggcaat gccttttttg tcagttgttt gtgtcgttgt ctgttttttt
     1501 ccatctaaaa gagtgccttc cagagtaacg ttgacgcctt cgattggttc agtgccatct
     1561 ctcacccacc aaccgcggtc aaatgcggcg acgcggagta catagcgatt atcgggcttg
     1621 cgcttaatct caatatcttg ggtaataact tggtcggtat cttcggtgaa ggtaatagtg
     1681 tacacgtcac ttagtttgcc cttttggtct tccgcccaaa gcttgtaggt acctttgaca
     1741 aacttctgat cccagagctc gtattctcca gttttatcgt ccgaatctgt cgaatcaaca
     1801 ttctctgcga caacggtaat gccgccaagt ggtttttttg cgccatggtc catttcatag
     1861 gtcatgccat cgacttcatt gtcgttggag aaacgcatcg taaaattatg ggtgttggtc
     1921 ccgcttacta agtccaagtc gtagttatat ggtttaaatt taggttttgt tgcttggagg
     1981 tgggagaact catcgccgct gatggttgga aacttgtagt atcctttgtt attggtcgtt
     2041 gtggtgaact ttttcgttcc ttgagtcagc tcaatagtta ctccttcaag ccccacacca
     2101 ctttctttgt aggtaaccgt cccctctaaa ctgacgaaca tggtgggaga gggactcggt
     2161 gacgggcttg ggctgggagt cgccggggat acgctagggc tgagtgtcgc tgcttggacc
     2221 gaggataagg taacgacaca aaataataag aaagaagtca gatatcggag aaaagtaagg
     2281 caaagtcgct gtgtcatcat gagtatcctg gtagtattct cttactataa agactgcttg
     2341 cttgtttcac aagtaagtac gagtcaagct tctctcattg acaaaacttt ctccctcggg
     2401 tatcatatag ctacacttat tcgttctctc tcatttgcgc tgtgcgcacg acgacctgtc
     2461 gtaaccgatt aactatggat ttacttaacc gactttaatc gcgtcaaagt acctctcctg
     2521 aggatttttt gtgtacgact tgagaaccaa taattatcaa tgaaaacaaa acacgtttta
     2581 tagaaacgaa aggattctat ggaagaaaat aattcttcta cgccgcacag taacgaggat
     2641 cgtcgtccga atcggcgtcc tcagcggcca atgtcccgtg ctccgcgcat gaatgcgcct
     2701 cacgggcact cagttggtga tggaaacgca tcttcagctc aaaaacaatc aaatacccaa
     2761 gaccggttta tgcgtccgag tttaagccga cacggcgccc gccctgccgg tggtgatcgt
     2821 atgcagcagg ctaataaccg cggctttgtt gcgccatcgg aacgaacaca agatcgttcg
     2881 aatggatttc cccgttcacc ccaacgcaca cctgccgccc gtaaccctcg tctggctgac
     2941 ggaatgcagc cccaggtaat gcgacggccg aacacacgtc gtcacgcccc tgatggacag
     3001 cctgcaagtg ccggtggtaa tggtggcgag cagcggctca accgtcgccg tttttctccg
     3061 cctcgcggtg gtgataataa atatgccatg gtaacgcaga tcaccgaaga cgcaaaaaac
     3121 agctcaacga agcaagttgt cttaagttct gcctcccgta agttacgcat tattcctttg
     3181 ggtggtgcgg gtgagacggg ttctaaaaac atgactgttt ttgaatatgg cgaag
//
LOCUS       DASWYB010000023         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_379550, whole genome shotgun sequence.
ACCESSION   DASWYB010000023 DASWYB010000000
VERSION     DASWYB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379550"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            517..602
                     /locus_tag="VGE59_01210"
     tRNA            517..602
                     /locus_tag="VGE59_01210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:550..552,aa:Leu,seq:caa)
     gene            718..1161
                     /locus_tag="VGE59_01215"
     CDS             718..1161
                     /locus_tag="VGE59_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074304.1"
                     /translation="MQLRQISPEDRAEVLRRAIRTQTELDEQKARTDALQEAVEELGI
                     SPDALALAVCQVQRRRARTAWLRRISLSCVAGLIAAVGLTQVKPTSQTTPPTVEQSVA
                     WNPTVDLPDIPGENTVTETNTATVSNDISLSVNTGNNTFVANTGF"
     gene            1271..1966
                     /locus_tag="VGE59_01220"
     CDS             1271..1966
                     /locus_tag="VGE59_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HEY1074305.1"
                     /translation="MPLSHKTQKKYRWYTILAIVEGLFVFTALFLAIFIYANWGAVSN
                     EFAFAFKSPTAAPTVTSTPAATATPVYEPAHIIIEKLGVDTPITWDVSAEDTVEYLNK
                     GVAHLQGSAHLGEVGNVFITGHSSDYAWKKNPYAAVFSLVPKLVPGDKITIREHGKAY
                     VYQVAEVKVVHPDQVEVANETTSPVLTLLTCYPVGTSRDRFVVHASLISSPEKPVEVE
                     QGKSYTLPAIKFR"
     gene            complement(1970..2710)
                     /locus_tag="VGE59_01225"
     CDS             complement(1970..2710)
                     /locus_tag="VGE59_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEY1074306.1"
                     /translation="MARPRSSFRSIFNAFLTVLLFVIISSTLVGLTNGYTYDFSSNQF
                     TQTGIISLTVKQMPVTVSIDGKKKLYKKTPINLTYLVPGQYTVEIRKEGYYPWVKTFR
                     IKAGEVKVNPFVRLYLTEINPQPATSKEADMVANATMVAPDLDIRLYEIWIKPISRTY
                     PFTVTSDRFAFVGRYAKPVHGAIWTEDKTNIIFQVGNEIRLIDRDGTNDHLLVSLSSD
                     QATRFAITSDGNALIYQDGDMMYRRQLQ"
BASE COUNT          797 a          724 c          676 g          749 t
ORIGIN      
        1 cacgctactt tttctgtggg ctgcactcac tatagcatat ctttttgacc atgcaataag
       61 ccgcctgcac aagtgtgtcg aggcggccgt cgtaaagata caaatgtttc gccggaggtg
      121 gagcgagccg ttgggacact tttggtttac acaaaagtga accgtgctca ctccaccccc
      181 gcacggggca gagacaaatc gaggaccgca gtcctccgcc ctttgtcctg cctatgctac
      241 tggactccca atgaatagag ggcgcacgtc tatcaccata ttcaccgccc agcagcggag
      301 aatccgctag tgcttcaaga agctcacgaa gccggtcatc gcacaagacc agctccgaac
      361 taacggagtt acgctcagta cccagtccgc cccgttctga ccccccacca acggactgtt
      421 tacctcgcga tcacaaatga attatactat aaaatatttt attgcacaat gtctttgact
      481 agatttttta ctatcttcag gtataataat ggcattgggc gagtggcgaa atggcagccg
      541 cgatggactc aaaatccatt gtcttcacgg acgtgtgggt tcgactccct cctcgcccac
      601 caacaaacgg cttcggttta ttcgaagccg tttgtttttt atgattattc acattgaact
      661 ctcttcactg aacgagttag catgattgta gcgccaaacg tatgtactgg aggtatcatg
      721 cagcttcgcc agatctctcc tgaagatcgt gctgaagtcc ttaggagggc gattcgcacc
      781 caaactgagc tcgatgagca aaaggctcgg acagatgcgt tacaggaggc agttgaagag
      841 ctagggatat ctccggatgc cttagctttg gcagtctgcc aggtccagag gcgtcgggct
      901 cgcacagcct ggcttcgacg gatcagtctt tcctgtgtag ctggtctcat agcagccgtt
      961 gggctcacgc aagtaaaacc gacaagccaa acaacaccac ctaccgtcga acagtctgta
     1021 gcgtggaatc caaccgtcga cctccccgac ataccagggg agaataccgt gacagaaacc
     1081 aacacggcga cagtgagcaa cgacataagc ctcagcgtta acacagggaa caacacattc
     1141 gttgccaaca ctggtttctg agaaagatgt gccatcctcc tcgtggagaa attgtccctg
     1201 cgctaactaa cggtgcaggg acgtccttta tcctcatgac aattgcttca ctctttgcta
     1261 taattcgtat atgcctttaa gccataagac acaaaagaaa tatcgctggt ataccatttt
     1321 agccattgtt gaagggctgt ttgtttttac ggccttattt ttagccattt ttatatacgc
     1381 gaactggggc gcagtcagca atgaatttgc attcgctttt aaatcaccca cagcggcacc
     1441 aacggttacc tcaactcccg ccgccactgc tacaccagtg tatgaaccag ctcatattat
     1501 cattgaaaaa ctcggtgtcg acacacccat tacctgggat gtctcggccg aagatacggt
     1561 tgaatatctt aataaaggcg tagcacacct ccaggggagt gcccatttgg gagaagttgg
     1621 gaacgtcttt atcactggtc actcatcgga ttatgcttgg aagaaaaacc cctacgctgc
     1681 ggtcttttct cttgtaccaa agttggttcc cggcgataaa atcactattc gcgaacatgg
     1741 gaaagcgtac gtctaccagg tcgcagaagt caaagtcgta caccccgacc aagttgaagt
     1801 agccaacgaa acaacctcac cggtcttaac tcttttaacc tgttatcccg ttggcacatc
     1861 ccgggatcga tttgtagtac atgccagttt aatctccagc ccggagaaac ctgttgaagt
     1921 agaacaaggt aaaagctaca ccctacccgc gattaaattt cgataatatt tactgcaatt
     1981 ggcggcgata catcatgtcg ccatcctgat aaataagtgc attgccatca ctggttattg
     2041 caaaacgggt agcttggtcg ctcgaaagac ttacaagcaa atgatcattc gtaccgtctc
     2101 ggtctatgag acggatttcg ttaccgactt gaaaaataat atttgtcttg tcttcggtcc
     2161 agattgcacc atgaactggc tttgcataac gaccaacaaa ggcaaaccga tccgaggtga
     2221 cagtaaaagg atacgtgcgg gaaattggct tgatccaaat ctcataaaga cggatatcta
     2281 ggtcaggcgc aaccattgtg gcattagcca ccatgtcggc ttctttcgat gttgctggct
     2341 gcggattaat ctctgttaag tagagccgca caaatgggtt aacctttact tcgcctgctt
     2401 taattctgaa tgtcttgacc cagggatagt agccctcttt gcgaatctcg acagtatatt
     2461 ggccaggcac taagtaagtt agattaatag gtgttttttt atacagcttt tttttaccat
     2521 caattgaaac cgttacgggc atttgtttga cggtgagact gataatacct gtctgggtaa
     2581 actgattact actaaaatca taggtgtagc cattcgtaag gcccaccaaa gtagaggaaa
     2641 taatcacaaa aagtagaacc gtaagaaacg cattgaagat cgaacggaat gaggaacgag
     2701 gtcgggccat atgtagtata agtatgcggc atctagcaga gagggtcaac ctcgtttggc
     2761 attcagccga cagagtgcta tggtaggcct ataactaact gattgaaaag cgaggttgat
     2821 tatgagcgtg aagtacgtca ttgaaggtgg tcataagctt cgaggagaga ttaccgttaa
     2881 cggttcaaag aatgccgcac tgccaatgct ggcagcaact cttttgactt ccgagaaatg
     2941 taccct
//
LOCUS       DASWYB010000024         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_734314, whole genome shotgun sequence.
ACCESSION   DASWYB010000024 DASWYB010000000
VERSION     DASWYB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734314"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGE59_01230"
     CDS             complement(<1..281)
                     /locus_tag="VGE59_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074307.1"
                     /translation="MKKILSAFVYISATLFILQTVIKLTTYNVHFVHACRTDDSITNC
                     GTYKATIVFDPMDGRYITSMYDLTTGTTITFNPISHEDSYEQGCWLGTKR"
     gene            complement(278..490)
                     /locus_tag="VGE59_01235"
     CDS             complement(278..490)
                     /locus_tag="VGE59_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1074308.1"
                     /translation="MAIIINLDVMLAKRKMSLTELSEKVGITMANLSILKTGKAKAIR
                     LSTLETICKVLDCQPGDILEYRPEHR"
     gene            complement(502..969)
                     /locus_tag="VGE59_01240"
     CDS             complement(502..969)
                     /locus_tag="VGE59_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEY1074309.1"
                     /translation="MLKQGSTLILKTTVLLIGLVALALCLFVLPIGLRGDSSGYYRPI
                     ILGMYVTAIPFFIALHQAMKLLGSIDTHAAFSNASVQILKNIRNCALTISALYAVGLP
                     YIYTVADKDDAPGVMAVGLIIVFASFVIATFAGVLQKLLKNALEIKSENDLTV"
     gene            1057..1353
                     /locus_tag="VGE59_01245"
     CDS             1057..1353
                     /locus_tag="VGE59_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074310.1"
                     /translation="MTKNPIINAFAALVYIALVASAMYYGSSYAGGEDNVFAPIAMLS
                     LFTLSAAVMGYLFLVGPALLYLEGKKKEGITFFLHTLGAFAVITAAAFLLFVLM"
     gene            1482..3116
                     /locus_tag="VGE59_01250"
     CDS             1482..3116
                     /locus_tag="VGE59_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1074311.1"
                     /translation="MHTSNWKKWGSLVVLSMALAIIVIDTTLLNVSLGTIIREFNTTI
                     QGIQWVITAYALTLSALTITGGRLGDLFGRKRMFMLGAVIFAVGSYVASISHNVPTMI
                     LGESIIEGVGAALMMPATSSLLLSTFEGRERAIAFGVWGAIAAAAAALGPIIGGFLTT
                     HYSWRWGFRINIVVAAILLLGSILVKEARDTKEKPSLDWGGVFLSSIGLLLIVFGVIE
                     ASTYGWWFAKSAFTLGSFSLPTWAGLSVTPYSIILGVILLVLFGLWERYMESKGRTPL
                     VSLGIFQNQQFAAGSATTAVMSLSMTGMIFSLPVFLQAVRGLDAFQTGMSLLPMSLTI
                     LVVAPLSGFLSTKFRPKYMIQLGLFLNMLGVLVLRQSMTVDSTVGHLAPGLMIFGAGL
                     GLVMAQISNLTLSAVPLHLAGEASGINNTLRQLGSTLGSAIIGAVMLSALSANLVNGI
                     TASSVIPTSMKTAVAQEISQQTSAVEFGGGVQTREGIPPVVTAEIHRISNQSMVDAAR
                     VTFVYTALFSFLGFLTAFSLPNIKASSRQGAPTAGH"
     gene            3210..3734
                     /locus_tag="VGE59_01255"
     CDS             3210..3734
                     /locus_tag="VGE59_01255"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY1074312.1"
                     /translation="MITQHQQEQLMREAIHEAKRGEREGEAPIGCVLAVLQDGQVDIV
                     ARGHNQMNRLHCRVAHAEIRAFENATNTSVPAVDLDAEGSILVSTLEPCVMCMGAAIE
                     AGVKYVVFGLSSPADSGTQRVQETVFSPESSQPIVFGNVLERECRKLFADWLPQAKNP
                     SQVLFVSQLLALTS"
     gene            complement(3844..4407)
                     /locus_tag="VGE59_01260"
     CDS             complement(3844..4407)
                     /locus_tag="VGE59_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074313.1"
                     /translation="MESVLRLVIALLAVITTLVLSAFAEWAGHAVMHHKGINRLINLW
                     LPWLPNRHLLEHHGDYMGEAFSHDPPSKYKLSLSVIGVTAIEIALLLILQPILGSLVT
                     KVVIVVSVGYLTCYEGVHYLLHNHCPRLGRLYATDLFHSMEKHHRGHHHRAGVNFGIL
                     PSGRKFDEWFGTIGLENTTPEPPHPST"
     gene            4558..4707
                     /locus_tag="VGE59_01265"
     CDS             4558..4707
                     /locus_tag="VGE59_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074314.1"
                     /translation="MKKNKAWHHANELPDEATLQQQIAWHLAHQKYCACKEIPKTVLK
                     ALKKK"
     gene            4771..5190
                     /locus_tag="VGE59_01270"
     CDS             4771..5190
                     /locus_tag="VGE59_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007389150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1074315.1"
                     /translation="MPTFTLDAYLFFTGNCREAMEFYKEVFGGELSVQTYGAAQSDLP
                     EEMKHMGDKVMHALLNGGLIRLMASDSTRDKFEDSFISLALGGTDEAKLREVFEKLSD
                     GGKVTQPLKKESWGDVFGSLTDKFGVDWMVNISTEKA"
BASE COUNT         1343 a         1103 c         1349 g         1535 t
ORIGIN      
        1 cgttttgtac ccagccaaca gccttgttcg taactgtcct cgtgagagat ggggttaaaa
       61 gtgatagtgg taccggtggt taggtcgtac attgaggtga tataccggcc gtccatagga
      121 tcgaatacaa tggtagcttt gtatgtgcca cagtttgtga ttgaatcatc tgttcggcac
      181 gcatgcacaa aatgaacatt gtaggttgta agtttgataa ctgtttgcaa gatgaataag
      241 gttgccgaga tataaacaaa agcagagagt atttttttca tctatgctcc gggcggtact
      301 ccaggatatc tcctggctgg caatcaagca ctttgcaaat tgtctcgagt gttgataatc
      361 gaatggcttt ggcttttcct gtcttcagaa tggaaaggtt tgccatggtg atgccaactt
      421 tttcggaaag ttccgtcagg ctcattttcc gtttagcgag cataacgtca aggttaatta
      481 taatagccat agttttaatc cttagacagt caggtcgttt tctgatttta tctcgagggc
      541 attttttaga agcttttgta gtacgccggc aaaggttgct atcacgaatg aagcaaagac
      601 aataataagc ccaactgcca tgacacctgg agcatcgtct ttgtcggcaa cagtgtagat
      661 atatggcaag ccaactgcat agagggcgct gatagttagg gcgcaatttc gaatattttt
      721 taagatctga actgacgcat tggaaaaggc ggcgtgtgta tcaatggatc ctaaaagttt
      781 catcgcctga tggagtgcga taaagaatgg tattgcggtc acatacattc ccaatataat
      841 agggcgataa tatcccgaac tgtcgcctcg aagtccaata ggcaatacaa agagacacag
      901 agctagagcc acaagtccga tgagtaaaac agtcgtcttg agaataagag ttgatccttg
      961 tttaagcata atacctcact atttaattgt attgtgctca ttctagtaca aaaattatcg
     1021 atttgcaata aatttatatt gatgtataat atgcccatga caaaaaatcc aattatcaac
     1081 gcgttcgctg ctcttgtgta tatcgccctt gttgcctcgg caatgtatta tggttccagt
     1141 tatgcggggg gcgaggataa cgttttcgct cctattgcca tgctttcgct ttttacgctc
     1201 tcggccgcgg tgatggggta tctttttttg gtgggaccgg ctttattgta cttagaaggt
     1261 aagaaaaaag aaggaatcac ctttttcttg cacacgcttg gggcgtttgc cgttattacg
     1321 gcagcagctt ttctgttgtt tgtgttgatg taacgtttat agggctccat aacttaggaa
     1381 aaacatgaaa tagtgtgagt attttattat gtagtttttt ttcttggcgc tgtgcgattt
     1441 ttcccttaca attaatgagg tgaaagaacg aaaggatagg tatgcataca agtaattgga
     1501 aaaagtgggg aagtttagtg gtattaagta tggcgctggc gattattgtc attgacacta
     1561 ctcttttaaa tgtttcgttg ggcacgatta ttcgtgaatt taatacaacc atccaaggga
     1621 ttcagtgggt tattactgcc tacgcactca cgctgtcggc acttactatt acgggtggcc
     1681 gattgggaga cttatttggt cgtaagcgga tgtttatgct tggagccgtt atttttgccg
     1741 ttggttcgta tgtggcttcg ataagtcaca acgttccaac catgatactc ggcgagtcta
     1801 ttattgaagg agtgggagcg gccttaatga tgccagctac ctcttcatta ttactttcga
     1861 cgtttgaagg gcgtgagaga gcaattgctt ttggagtctg gggcgcgatt gccgccgcgg
     1921 ccgcggcact cgggccaatt attggtggat ttcttaccac acattactcg tggcggtggg
     1981 gattccgtat taacattgtg gtcgcagcga tcctgttgct cgggtctatc ctagtgaaag
     2041 aagcgcgtga cacaaaagag aagccttctc tcgactgggg tggcgtattt ttgtcgtcca
     2101 ttggattgct cctgattgtt tttggggtga ttgaagcttc gacctatggc tggtggtttg
     2161 ctaagtcggc attcacgctt ggttcgttct ctttgccaac ctgggcagga ttatcggtga
     2221 ctccttattc gattattctt ggggtcattt tattggtcct ctttggtctc tgggaacgtt
     2281 acatggaatc aaaaggccgc acacccttag tctctctggg aatctttcag aaccagcagt
     2341 ttgcggctgg ttcggcaaca acggcggtga tgtcactctc gatgacgggg atgatttttt
     2401 ctctaccggt atttcttcag gcggtacgcg gactcgacgc cttccaaacg ggcatgagtt
     2461 tgttgccaat gtcgctcacc attcttgttg tcgctcctct atcaggattc ttaagtacca
     2521 agtttcgtcc gaagtacatg attcaacttg gtcttttcct gaacatgctt ggagtgctcg
     2581 tattgcgaca gtctatgaca gttgattcta cagttggtca cttagctccg ggactgatga
     2641 tctttggtgc tgggctcggt ttggtcatgg ctcaaattag caaccttacc ctttcggctg
     2701 tgcctcttca cttagcaggg gaagcatcgg gtattaataa tacgctgcgt caacttggtt
     2761 caacattagg ctcggcaatt attggcgcgg taatgctttc ggctctctct gctaacttag
     2821 taaatggaat taccgccagt tcagtaatcc caacatcaat gaaaactgct gtcgcacaag
     2881 aaatatccca acagacaagc gccgtagagt ttggcggcgg tgtacaaaca cgggaaggta
     2941 ttcctcccgt tgttactgcc gaaatacatc ggattagtaa tcagtcgatg gtagacgctg
     3001 ctcgggttac ttttgtctac accgcgctct ttagtttcct cggttttctc actgcctttt
     3061 cgctcccaaa tataaaagcc agtagccgtc agggggcgcc aacggcaggg cattaggaag
     3121 tcttccgctc tccttgagaa ttctcgtatt tctaccagaa tggcagcgcc tgtcttagag
     3181 gatagctgct tttttggtat agtgggggca tgattactca gcatcaacaa gaacagttaa
     3241 tgagagaagc aattcatgaa gcgaaacggg gagaaagaga aggcgaagca ccgattggtt
     3301 gtgtgttagc cgtcttacaa gacggacagg ttgatattgt tgcacgaggt cataaccaga
     3361 tgaatcgtct ccattgtcga gttgcccatg ccgaaatcag ggcgttcgag aatgctacta
     3421 atacctctgt ccccgccgta gacttagacg cagaaggttc tattcttgtt tcaaccctag
     3481 agccgtgtgt tatgtgcatg ggcgcggcaa tagaggctgg ggtgaaatat gttgtctttg
     3541 gattatcttc gccggctgat agtggtacgc agcgagttca agaaacggtc tttagtccgg
     3601 aaagttcgca gccaattgtt tttggtaatg tattagagcg ggaatgtcga aaactttttg
     3661 cagactggct gccacaggca aagaatccgt cacaagtatt atttgtgtcg caactgctcg
     3721 ctctaacgtc atagtatggg attctaatgg gacacgagaa taagaaaagc ctccgcagat
     3781 tggctgggag gcttttcttg cggaggcaga attatgggat gcgccctatt ttagggcctt
     3841 ggtctatgtg ctcgggtgag gaggttcagg tgtggtgttt tcgagcccaa tcgtaccgaa
     3901 ccactcgtcg aactttctgc cagacgggag tatcccgaag tttacccccg ctcggtgatg
     3961 atggccccga tggtgtttct ccatggagtg aaataagtct gttgcataga gtcttcccaa
     4021 gcgggggcag tggttgtgga ggaggtagtg taccccttca taacacgtta ggtaccccac
     4081 cgatacgacg ataactacct tagtgaccag tgaccctagt atcggctgga ggataaggag
     4141 taaggcgatc tcaatcgcag ttactccaat gactgaaaga ctgagcttgt acttgctggg
     4201 aggatcatgt gagaatgcct ctcccatata gtctccatga tgctcgagaa ggtggcggtt
     4261 gggaagccaa gggagccaca ggttgataag ccggttgata cccttgtggt gcattactgc
     4321 gtgtcctgcc cactcggcaa atgcagaaag cacgagtgtt gttatcaccg cgagtaatgc
     4381 aataaccaaa cgaagaacag actccatact gagatcctcg gaccgaaagg tctatccaag
     4441 acggatgcat tacatatata ctctatttgg ctgtttttgt caatttgttc ggtgatgcaa
     4501 agtgggtagt tgcgacctag cttcgacggt ggtgagtata tttgatataa tagccatatg
     4561 aaaaagaata aagcttggca tcacgccaat gaacttccag acgaagcaac gctccaacag
     4621 caaattgctt ggcacttagc acaccaaaag tattgtgcat gcaaagaaat tcccaaaaca
     4681 gtgctcaagg cactcaagaa aaaataacgg tgactccacc gttattttat gagatactag
     4741 cggcacaatc taaaaagtag aggagtttgt atgccaacat tcaccttgga tgcgtatcta
     4801 tttttcacag gcaattgccg tgaagcaatg gagttttaca aagaagtatt tggtggagag
     4861 ctttcggtgc agacttacgg ggccgcgcaa tcagatcttc ccgaagaaat gaagcacatg
     4921 ggcgataaag taatgcatgc ccttttaaat ggtggcctta tccgtctcat ggccagcgac
     4981 agtactcgag ataagtttga agatagcttt attagtttag cccttggtgg gaccgatgag
     5041 gccaagctcc gggaagtgtt tgaaaaactt tctgatggcg gtaaagtaac tcagccgctc
     5101 aaaaaagaat cgtggggaga tgtgttcggt agtcttaccg ataaattcgg tgttgactgg
     5161 atggtaaata ttagcaccga aaaagcataa gaacaaaaga ttctaaagaa gcagcatagt
     5221 tgcggcttct ttagaatggt gcgttggaag gagctgttat aaacactgcc gactctcaca
     5281 acactttttc ttatctctca gctccggcaa ggcgagcgct cgcccgtgct
//
LOCUS       DASWYB010000025         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_322713, whole genome shotgun sequence.
ACCESSION   DASWYB010000025 DASWYB010000000
VERSION     DASWYB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322713"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VGE59_01275"
     CDS             <1..690
                     /locus_tag="VGE59_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074316.1"
                     /translation="NFVSAQESSSSPTATVTAQSTASPETEIMTTAVQTPILSEQRKV
                     ERVKRANEVKERAKAMVTELKEAQKTKRLDMQEKISALKDTRKKTQAQKLTTQIAHVN
                     DVLSKRQLTFISSLENILDSIEDRTDKTEYAGGNVSTVRAQLVVTRAALEAVKEKVLT
                     QQEKEYVVDITTEDALGQAYRVTVHQLRDDHRALRTELKSVAQKVRATYTLLKEVSQP
                     LGVSASPSAGL"
     gene            700..951
                     /locus_tag="VGE59_01280"
     CDS             700..951
                     /locus_tag="VGE59_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074317.1"
                     /translation="MNSKVTTVVLILSLVFLVGVVVFAFQRSRVLLRSANQISTTTIV
                     SPSSTPGDSLTEIETDINSTTLDETESDITTLEQEAENF"
     gene            complement(948..2102)
                     /locus_tag="VGE59_01285"
     CDS             complement(948..2102)
                     /locus_tag="VGE59_01285"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY1074318.1"
                     /translation="MCSRLRRDAHYFLIILGLLLIIGVLGFPTLKQTAVQNYFSPAYD
                     ADKENYNWEISEYATGLDVPWDMAFSPDGKLFITERPGNVKLRLSDGTIKTIAHLPQV
                     SAVGESGLTGIALHPHFTENNFVYLYYTYRDDGTILNRVSRYTFQNNQLINETYIVNN
                     LKGGSIHNGGRLRFGPDEKLWVLTGDAARPNLAQDPTAREGKVLRMNEDGSAPEDNPT
                     KGSLVYSLGHRNPQGLDWHPLTEELIVSSHGETAHDELNLVTPNGNYGWPVEKRCNEQ
                     APYIPPLLCSGEETWAPSGIAFWGTDIWRFRYSLFFAGLRSGKLQRIAIIDSKVAEQE
                     TIIDGKYGRLRGLVKGPDGALYVSTSNKDGRGKPETTDDRILRVVPKLIP"
     gene            complement(2172..2247)
                     /locus_tag="VGE59_01290"
     tRNA            complement(2172..2247)
                     /locus_tag="VGE59_01290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2212..2214),aa:Pro,seq:tgg)
     gene            2336..>3152
                     /locus_tag="VGE59_01295"
     CDS             2336..>3152
                     /locus_tag="VGE59_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEY1074319.1"
                     /translation="MKKLTSPKQKIALLVAMAVCLSLIGILVWAVLRYVEIRQDLDFV
                     YSDRDISIPLPGSSQTAMTPDDISSSSVPTTVVTAPSPSLTPVSLQRVNLDVPFTSQA
                     PESNWDSVHEETCEEASLLMAQWWAQGKTGESTEGYMNRIPPAVAEASLQDLVTWQKQ
                     TFGYFEDTTATETLRVAQEKLGLKTARLLTDVTQESLKAELAAGNILLVPAAGQVLKN
                     PYFKQPGPPYHMILVRGYNDTGFITNDPGTRRGEGFVYSYENLLDSIHDWTGDG"
BASE COUNT          874 a          708 c          741 g          829 t
ORIGIN      
        1 aattttgtat ccgcgcagga aagttcttcg tctcctacgg caacggttac cgcccaatct
       61 acggcatctc cagaaaccga gattatgacg acggctgttc aaactcccat cctttcagag
      121 caacgcaaag tcgaacgtgt aaagcgtgcc aacgaggtga aagagcgcgc caaagcaatg
      181 gtaactgaac ttaaagaagc gcaaaaaaca aaacgtctgg acatgcaaga gaaaatttcc
      241 gctcttaagg atactcgtaa aaaaactcaa gcacagaagt tgacaacaca aattgcacat
      301 gtgaatgatg ttctctcaaa aaggcaacta acttttatta gtagtttaga aaatatcctt
      361 gacagcatcg aggatcgaac ggataaaaca gagtatgcgg gtggcaacgt atcgactgtc
      421 cgggcacaat tggtagttac ccgggcggca ttggaagcag taaaagaaaa agttctcacc
      481 cagcaagaaa aggagtatgt tgtcgacatt acgactgaag atgccttggg tcaggcctat
      541 cgagtgactg tccatcagct acgggatgac catcgggcgt tacgaacaga attaaaatca
      601 gttgctcaaa aagtgcgtgc cacgtatacg ctattaaaag aagtttcaca gccacttgga
      661 gtgtctgcgt caccttctgc cgggttataa ggagctacta tgaactctaa agttacgact
      721 gttgtcttaa ttcttagttt agtctttctg gtgggcgttg ttgtctttgc ttttcagcgt
      781 tctcgggtgt tgctacgttc ggctaatcaa atttcgacca caactattgt ttcgccgagt
      841 tcaacgcctg gcgacagtct tacggaaata gaaacagata ttaatagtac aacattagac
      901 gaaacagagt cagatattac gactctcgag caagaggccg aaaactttta agggataagc
      961 ttcggcacta cacgcaggat tctatcgtcg gtagtttcgg gtttgccccg gccgtcttta
     1021 ttagaagtag aaacgtagag agcgccatct ggtcctttga ctaaaccgcg cagtcgtccg
     1081 tattttccat caataatagt ttcttgttcg gcgactttac tgtcaattat ggcgatgcgt
     1141 tgtaatttgc cactgcgtaa tccggcgaaa aagagggagt aacgaaaccg ccaaatatct
     1201 gtgccccaaa aagcgatccc ggaaggagcc catgtttctt ctccactgca cagcagagga
     1261 gggatatagg gcgcttgctc gttacatcgt ttttcgactg gccaaccata attgccgttc
     1321 ggtgtcacaa ggttaagttc gtcatgagcg gtttccccgt gtgaagagac aatgagttct
     1381 tcggtaaggg ggtgccagtc caacccttgt ggattgcggt gtccgagaga atacacaaga
     1441 gacccttttg tggggttgtc ttctggagcg cttccatctt cgttcatacg cagaaccttg
     1501 ccttctcgtg cggttggatc ttgggctaga ttaggacggg ccgcatctcc ggtgagcacc
     1561 cataactttt catcgggacc aaatcggaga cgccccccgt tatgaattga cccacctttt
     1621 aaattgttca caatataagt ttcgtttata agctggttat tttggaaggt ataacgagat
     1681 acacgattga gaatggttcc atcatcgcga taggtgtaat aaaggtaaac aaagttgttt
     1741 tctgtaaaat gagggtgaag cgcaatcccg gttagtccac tttctcccac agctgaaacc
     1801 tggggtagat gggcgattgt tttgattgtc ccgtcagaaa gtcgtagttt tacattccct
     1861 ggccgttcag taataaagag ttttccatca ggtgaaaaag ccatatccca tggtacgtcg
     1921 agtcccgtag cgtattcgct aatttcccaa ttatagttct ccttgtctgc atcgtaggca
     1981 ggcgagaagt aattttgtac ggcagtctgt ttaagagtgg gaaatccaag aactccaata
     2041 attagtaaga ggccaagaat aataaggaaa tagtgggcat cgcggcgaag tctggaacac
     2101 atattccccc tatagcaata aagcagagaa attgcaaaac aaaaaagacg gttttaaacc
     2161 gtcttctctg ctggtcgggg tgaaaggact cgaaccttcg acctcatggt cccaaaccac
     2221 gcgcgctacc aactgcgcca caccccgaaa tagtatccta tcaacagaaa aattatagct
     2281 tatttttcaa atattagcaa ttgtctcggc tcagaaataa agtatgataa agataatgaa
     2341 aaaactcact tcgcccaaac aaaaaatagc tttactcgtc gccatggcgg tctgcttatc
     2401 gcttattggg attcttgtgt gggcagtttt gcgctacgtg gaaattaggc aggatttaga
     2461 ttttgtttac agtgatcgtg acatctcaat tcctcttcct ggatctagcc aaacggcgat
     2521 gactccggat gatatttcgt caagctctgt cccgactacg gtagtaacag ccccctctcc
     2581 ctctcttaca ccagtttctc ttcaacgggt taacttagat gttcccttta cttctcaagc
     2641 tcccgagtcc aattgggata gtgttcacga agaaacgtgt gaggaagcgt cgctccttat
     2701 ggcccaatgg tgggcacaag gtaagacagg cgaatcaaca gaggggtata tgaaccgtat
     2761 ccctcctgcg gttgcggagg cctctcttca agatttggtt acctggcaaa aacaaacctt
     2821 tgggtatttt gaagatacaa ctgccacaga aacgttacgg gtggcgcaag agaagttggg
     2881 tcttaaaacg gctcgattac ttaccgacgt cacacaggaa tcactcaaag cagagctagc
     2941 ggcaggtaat atcctccttg ttcctgctgc tgggcaagta cttaaaaacc cttatttcaa
     3001 gcagccaggg cctccttacc atatgatact ggtacgaggg tataacgata cggggtttat
     3061 taccaatgat cctggtacgc ggcgtgggga agggtttgtc tattcatacg aaaatttgct
     3121 tgactctatt cacgattgga ccggtgacgg tg
//
LOCUS       DASWYB010000026        17434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_323213, whole genome shotgun sequence.
ACCESSION   DASWYB010000026 DASWYB010000000
VERSION     DASWYB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17434
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323213"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1534
                     /locus_tag="VGE59_01300"
     CDS             197..1534
                     /locus_tag="VGE59_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1074320.1"
                     /translation="MSLDLNPQFIRAYELMEQTEKHLFITGKAGTGKSTLLDYFRKQT
                     KKNCAVLAPTGVAAVNVRGQTIHSFFGFKPDITPDLIQKPSSRNGQLYKHLDCIIIDE
                     ISMVRADLLDCIDKFLRLNGRDKYAPFGGVQIIMIGDLYQLPPVVTLQDRELLSRRYR
                     SPYFFDADVFACLRLELVELEIIYRQQDETFIDVLNAIRTNTTTVDHFSRLNSQHQPL
                     GEATDGAITLTTTNALADGVNSDRLAQIAGAPITYEGQSSGNFLEKDAPAPRQLTLKI
                     GAQVMLLNNDSGKRWVNGSIGTITEIKSEIGEPDTIVVMLQDGRHVDVLPYSWESSEF
                     VLDDRSKVVTAKATGTFTQYPLKLAWAVTIHKSQGKTFDQVVIDLGRGTFAHGQLYVA
                     LSRCTTLEGIVLKQLVTRRHIVMDQRVVAFMQQFKETSVFRGEDAYEPAPQLF"
     gene            complement(1531..1986)
                     /locus_tag="VGE59_01305"
     CDS             complement(1531..1986)
                     /locus_tag="VGE59_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY1074321.1"
                     /translation="MQYWLIKSEPSVYSIDDLERDKTTYWDGIRNYQARNFLRDSMKV
                     GDKIIFYHSNTEPPAAVGVAEVSKEGYPDFTAWDKEDPHFDPKSDPSNPTWYMVDIAF
                     VKKFTHPLSLDAMKANPDLAGLMVIQKGARLSVQPVSKEHFERLVQLGS"
     gene            2084..2554
                     /locus_tag="VGE59_01310"
     CDS             2084..2554
                     /locus_tag="VGE59_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074322.1"
                     /translation="MDKGILSIIGVTIIAVGIVFAVAVNDSSKEASIAPRDKCVEHAG
                     LGMHIHPELEIRLEGQTVALPANIGVTSSCMHPLHTHDDSGKVHVEYPQKRDFQLGDF
                     FAVWNQPFSKSEILDKKVDETHTLTVTVDGALNEEYEKLVLKDGQKIVIDYHAK"
     gene            2647..2805
                     /locus_tag="VGE59_01315"
     CDS             2647..2805
                     /locus_tag="VGE59_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEY1074323.1"
                     /translation="MSLIELLAAILVPPLAVWLKVGLTTHFWINVILTLLGYLPGIIH
                     AFWVILTF"
     gene            2876..3145
                     /locus_tag="VGE59_01320"
     CDS             2876..3145
                     /locus_tag="VGE59_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEY1074324.1"
                     /translation="MAHKTYEELAEVLSLAQGQVEVGAKYQHYMSDEKFYTVMAVALQ
                     EETEAPCVVYQTSHSSGLIWVRNLDDWLSTVVKDGKSVPRFRRIE"
     gene            complement(3153..4283)
                     /locus_tag="VGE59_01325"
     CDS             complement(3153..4283)
                     /locus_tag="VGE59_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEY1074325.1"
                     /translation="MLKRFIYSILFVVSLLTPFSLSHAQGETVPSSVFYSVKVTNVSP
                     YTPSTELETLESSDAILISMQVTGGKDKGKFIDALWSNNDQTTRNFDVHSGDTLIATC
                     TLIDKQENCSIVSQQRSTGLILLVILFCIVTWAVTRIHGLRAILSMIIGLAVILFAIV
                     PLIVKGYSPLMVTILGGIIIIVPSLYISHGYKMKSHIALGSIFAMTVVIGLLSLGFSH
                     LLQLIGTGTEEALYLSRDINLQSVLLSAILLGTLGVIDDLALTQISIINELYHSNPSL
                     SASSLYHRAMKIGQDHISSVINTLFLAYAGVSLPLLILIQQSELPAFVALQQEQIATE
                     IMRTIIGTIGLILVVPLASYITAQLIIRKPEKFPAEAHHHSH"
     gene            4382..4813
                     /locus_tag="VGE59_01330"
     CDS             4382..4813
                     /locus_tag="VGE59_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY1074326.1"
                     /translation="MTSAHTILESLKLTGARMTRLRVALVGLFCDDITPVDYGVIANY
                     LLSQGIIVNKTTIYRELEYLLEHNVIQEVDLGERKKRYELVGHHHHHLICTGCEAVEA
                     VHLDDHLHDHELRILKEKKFKVTRHMLEFFGLCGMCQKKGS"
     gene            4815..5570
                     /locus_tag="VGE59_01335"
     CDS             4815..5570
                     /locus_tag="VGE59_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY1074327.1"
                     /translation="MSSTAYAFVSVGIVSLISFVGAVGLVLTRKSLQSSMFLLVSFAV
                     GVLFGDVFIHLLPESFEAFGDSLATSYFVLIGILLFFMLEKFIRWHHCHHGEDDHTHR
                     HHSLATMNLVGDGLHNFIDGLLIGASYLVSIPVGVATTVAVIAHEIPTEIGDFGVLLH
                     SGMSARKALLFNFYSALTAVVGVAVVLLLGNSVESFSAYLIPLTAGAFLYIAGSDLIP
                     ELHHHNPQVKHSIGQLLAIIAGITVMVLLAVFE"
     gene            complement(5605..6222)
                     /locus_tag="VGE59_01340"
     CDS             complement(5605..6222)
                     /locus_tag="VGE59_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074328.1"
                     /translation="MKAHNQKFLASCLATLVILLPILVWGSSLPRPLSQVSLYELFPV
                     FGLLAFSIFWLQLLGLLCKPYLTHLSLRPFFKWSSHAFMTFVIAHPLLLAIAQFNNHL
                     GLPLQSFYDYVPSNLTGYINLAVIAFLVFVITEITGWIGNTPWIQKFAKPLEYVNYLT
                     FFLVFWHSIHLGQHLQSGWLLTVWWFFVITGLPIITTLIIRDIKK"
     gene            complement(6231..6635)
                     /gene="msrB"
                     /locus_tag="VGE59_01345"
     CDS             complement(6231..6635)
                     /gene="msrB"
                     /locus_tag="VGE59_01345"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY1074329.1"
                     /translation="MEKIKKDNDTWKSELTPEQYRVLREKGTEPAFSGELLNNKEVGM
                     YICGACGNELFSSDTKFDSGSGWPSFWESIDSEKIELHHDDAYGMRRTEVVCARCGSH
                     LGHLFDDAPQTPTGQRYCINSASLAFRKKETK"
     gene            complement(6723..7199)
                     /gene="msrA"
                     /locus_tag="VGE59_01350"
     CDS             complement(6723..7199)
                     /gene="msrA"
                     /locus_tag="VGE59_01350"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY1074330.1"
                     /translation="MGKTHISMATFAAGCFWGVQETFDKVKGVKYSTTGYTGGTMEHP
                     TYEEVSSDTSGHAQAVQLSYDPDVVTYETLLTTFWDLHDPTTTNRQGPNIGSEYRSVI
                     FYHSPLQKAAAEKSKAEREALGTYSQPIMTEIVAAGPFWKAEARHQKYNDKHSISG"
     gene            7243..7692
                     /locus_tag="VGE59_01355"
     CDS             7243..7692
                     /locus_tag="VGE59_01355"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY1074331.1"
                     /translation="MEQFEIKHHVREVLNQEILISLATTDMRHQPWASVVHFTANDDF
                     TIYWLSDPKARHSENVETTKRAAGTVTLPLDADGKSIALQLEGTARRIVGDEVNMVRD
                     LTERRRGKPINPALLETRTFYALVPDRIYLLHEPLLGYERVELLLSA"
     gene            7823..9670
                     /locus_tag="VGE59_01360"
     CDS             7823..9670
                     /locus_tag="VGE59_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY1074332.1"
                     /translation="MEQMSKDTLLQESRTHVQSVQKAITQEIADMEQKVVSGEEEWRS
                     QSADDRVIFDTFLAHYQERKGELGQLYPSPYFVRCDVLFEGESEIKTYYFAKFPFSEE
                     QIYSWTVPAATLRYEEPGEVNYTALDGTVYHGTLVRKDQYMIVDGNVVFFASETAGRP
                     RELIYQEHFSTRKSGFVLPEIVAQMEKAQDQVVRAAHQGPFVISGPAGSGKTTLAFHR
                     VAYLMQSPDTASLYPSDSILILVQDTGTRDYFTHLLPELGIKQVAITTFFEWAVGVLE
                     LTGFEATKWSDLPEPSRDSYEYAKLKALREFVARPKKGTVYSWLEEAYASCFFPHHEI
                     LWQQQIKSRKLDRIDITLLLMAQNAEAGGLTHMVETFVKQRNGTYKRKFERQPIRYNL
                     MVVDEFQNYLPEQLTLLQGCRNPRLESTIYVGDMAQQVQLGTVKNWEEAREKIETDRM
                     IVLAKVYRNTQRILEFIRSLGYEVHIPEKIRIGDPVAEVLVESVEEEVAYIEDLLTRD
                     TQGSLGVIAKDSAYLAPFRTAFRGRDRVHIVTMQESQGVEFDRVCLVGITPELLTSHV
                     FEEAIRKEKRHIDRDLLYVALTRAMSEMHVLGNCYLRESIQFLDSSVVT"
     gene            9678..10250
                     /locus_tag="VGE59_01365"
     CDS             9678..10250
                     /locus_tag="VGE59_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074333.1"
                     /translation="MHIDLDPRRYRNWPYKNTTLLILSFLILWLIIDTHPVQRAVSQV
                     SGWGYLGAFLTGIFFVSVFTAAPAAVILFRLADVLNPWEVALFAGIGAMCGDYLIFRY
                     LQDKVFDELKPIFFKIGRTQLGQLFHTPYFAWLLPIVGAAIIASPLPDEAGIGLLGAS
                     QVRGWQFLLLTFVLNSVGIFLIVLLAQTAS"
     gene            10265..10600
                     /locus_tag="VGE59_01370"
     CDS             10265..10600
                     /locus_tag="VGE59_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY1074334.1"
                     /translation="MLLLWIINALTLLLVANYLPGFHVSGFYAALITALVLGLVNAVI
                     RPIVILFTLPINLLTLGLFTFVINGLMIWLVSTVVKGFEVDSFNTALWAALILWLISF
                     VTNALTKKA"
     gene            complement(10646..11941)
                     /locus_tag="VGE59_01375"
     CDS             complement(10646..11941)
                     /locus_tag="VGE59_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1074335.1"
                     /translation="MVRRVIHTYWLLAVVTNAATGFFAPIYTTYLMAKGLSLGQAQMV
                     NFVYFTICLTLEMPTGALADVFGRKRCYVISRIVIACAYFTYATASGVPGFIMAEVLA
                     GIGSCLASGAFQAWLRHSLTHHGYTGELDDITGKSYILNCVTGFATAQIGALSLDHNV
                     IRSVVDTLGKGVLASWIEHHFLVLPWVFGGSLMGCNAVIALVLMQDHHVSHQNLRGEI
                     WDHLQHLGRAMQRAVRYAVSSSDVRLIVCMALLFAVATQAPNMQWQPLMKGQYGFPQA
                     MMGVVWIGISLALASGGALCVRLKSTRFDRRYTLLCSLLVVGVGISTTPYLASPALMM
                     TAFLFNQMGRGVFRPMIDAYANDQIPKDQEDHRATVLSLTSMPSHLGSMLGLAFSARI
                     AEAYTIPVAWRASGMLLVVGACILLFVVTFKKSLATAKN"
     gene            complement(12180..13595)
                     /locus_tag="VGE59_01380"
     CDS             complement(12180..13595)
                     /locus_tag="VGE59_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY1074336.1"
                     /translation="MKAHIIGICGTGMAAVADLLLDHGWEVTGSDEGFYPPISTYLET
                     RPVTMLSPYAASNIPADVDTIIIGKHAKLVPDENEEVKAAFASGKPIKSYPDVIEELT
                     AHTKNFVVAGSFGKSTTTALTAWALSSAEKDPSYFVPALCPNLPNLGHKGGGGMFVLE
                     GDEYPSANWDTSSKFLHYNASTLLLTSCTHDHLNVFPTLESYLAPFEKLLIKLPKDAI
                     IMACLDGEHVTETLAKVKRTALTYSLNNKAADWWAEDITRDEITSFTLINKGKKIARL
                     SSRLLGDHNVLNMVGVAAWLLSNNAVTAESLEESFASFSGIRRRLELRTKKTSIPVYE
                     DYGSSPAKLLAGLTAIRAQFPHKRIRVLFEPHTFSFRNRAILPQYLTLFDQADTVFVF
                     QPPTHGAGTHDQLTQQEIVDQIASTGKKVRAFTTKEELEPFFKHILQPHHDVILMETS
                     GEMGGAMNFTVDYVTEHFSNA"
     gene            complement(13620..14192)
                     /locus_tag="VGE59_01385"
     CDS             complement(13620..14192)
                     /locus_tag="VGE59_01385"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011839601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY1074337.1"
                     /translation="MNKVAALAQVLEELKQLPLYEGANFVPGAGNPDTEILFVGEAPG
                     KKEDELGVPFVGAAGKFLNEMLESIGLKREDIFITNIVKQRPPENRDPTPEEIEKHWP
                     LLIKQIEIINPKIIVMLGRHSMSKLLPGVGTISKLHGKPFRRKDGRIYLPLYHPAAAL
                     YNGGLRQTLLDDFAKIPLIIKKIENKESTE"
     gene            complement(14214..15461)
                     /locus_tag="VGE59_01390"
     CDS             complement(14214..15461)
                     /locus_tag="VGE59_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074338.1"
                     /translation="MIHLPAPRDNQWLFLDMNAFFASCEQHHDPSLSGKPIAVAPSLG
                     PSACIIAASYEAKAHGITTGWRVGEAKQKLPELIVVPGNHRYYTDIHNRILSFLDAEV
                     SPGVKTLSVDEFGIPLDTREQWTPNAHALAIKVKEGISDIFSPALMCSVGVAPNMFLA
                     KVGTEIQKPNGLVVIQQHTIESAFSQLALREIPGINWGMSRRLNAIGIKTTVDFFRAP
                     QDLLHNAFGITGDAWWYNLHGYNLQHTNGFRASERPKSISHSHVLAPALRTKAKGRAV
                     LYKLWIKVADRLREKHLGTSRISIMARSHTDRWEHTLSLSPTQNVFDVFRHVAAAYDT
                     ELSDRFCPKQILVVCHSLTPHTPQPISLFDTTSHKVIDLFEASKNLNHKFGRWTVKPA
                     SLLIAGDAAPNRITFRKPDYEMD"
     gene            complement(15551..15931)
                     /locus_tag="VGE59_01395"
     CDS             complement(15551..15931)
                     /locus_tag="VGE59_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074339.1"
                     /translation="MQTKQALKVLGTFLFLLGVFGYIFPNWKSVHFTNNENLFHVISG
                     LIAITASNLKPRGRTITLGLLIVFYLAWGIWGFTLHAPLDAKILIKNKINLSTQLDTI
                     DNYLHILFGLGFAWSWLNRKHSQS"
     gene            16050..16259
                     /locus_tag="VGE59_01400"
     CDS             16050..16259
                     /locus_tag="VGE59_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074340.1"
                     /translation="MNAVLGRADEHISHWRDLQTAEPLGGVLAIDRLDGSSARAYTKL
                     EDPIFLRVHNPDSSETEEVLVLHSD"
     gene            complement(16374..>17434)
                     /locus_tag="VGE59_01405"
     CDS             complement(16374..>17434)
                     /locus_tag="VGE59_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HEY1074341.1"
                     /translation="GDIESRCIVHKGSFAEIVSLENIHQAWLEFRRGKRRKPDVQSYE
                     RNLEDNLFRLHFNLKRGIFPETWYEQFRINDPKPCLISKAPVECRLLHHAIYRVLYPL
                     YDRTFIFDSYSCRVGKGTHKAFERITEIARKVSKNYSGPCWALKFDIRKFFDSVDHVT
                     LFNLLSSRISDRKLLHLLDNIIGSFNMNEGKGMPLGNLTSQLFANVYLDPLDKFVKHH
                     LKAKYYLRYADDMILLSSDPDELIGYFVEINRFSKERLKLQIHPHKTTLRKLSWGIDF
                     VGYVALPHYTLPRRKTVKRATKRTLAKVKEGPKAVKPTLVSYVGYLCHANAIMVSKNL
                     IDLTVAKVRKNSAWFRNQ"
BASE COUNT         4554 a         3637 c         4311 g         4932 t
ORIGIN      
        1 ataagggaac cgtggaaaaa ccaacccact ttattattaa cgaccctatt tcgggtcgac
       61 tcacttggac agcgagtcaa ctcgcagctg actgggcaaa gtttgataat agcggcgtag
      121 tcgtgtactg ataggttcgg cacagactca tacgtatcgg ttgttattat tgcgttccta
      181 ggttaggata agatccatgt cacttgacct caatcctcaa tttattcgtg cctatgagtt
      241 aatggagcaa acagaaaagc atttgttcat tacgggtaaa gcaggaacag ggaaatcaac
      301 cttgctcgat tatttccgta agcaaaccaa gaaaaattgc gcggtcttag cgcctactgg
      361 cgttgcggcg gttaacgtac gtggtcaaac catacattca ttttttggat ttaaacccga
      421 tataacaccc gatttaatcc aaaaaccgtc cagtcgaaac ggacagctct ataaacatct
      481 cgactgcatt attatcgacg aaatttcgat ggtgcgggca gacctgcttg attgtattga
      541 taagttttta cgtctcaatg gccgtgataa gtacgccccc tttggcggcg tacaaattat
      601 tatgatcggt gacttgtatc agctccctcc tgtggtgaca ttacaagacc gagaattact
      661 ctctcgccga tatcgaagcc cttatttttt tgatgcagat gtttttgctt gtcttcgttt
      721 ggaactagtg gaactggaaa ttatctatcg gcaacaggac gaaactttta tcgatgtctt
      781 gaatgctatc cgaaccaata ccacgaccgt tgatcacttt agtcggctga atagtcagca
      841 ccagccattg ggcgaagcta ctgatggagc tattactttg acgaccacga atgccttagc
      901 ggatggtgtc aacagtgacc gtctggctca aatcgcgggg gcacccatta cctatgaagg
      961 acaatcgagc ggaaatttct tagaaaagga tgcgcctgct ccgcgtcagc ttacccttaa
     1021 aataggggcg caggttatgc tgcttaacaa cgactcgggt aaacgttggg tcaacggaag
     1081 tatcggtacg attacggaaa ttaaaagcga aattggcgag ccagatacga ttgttgtgat
     1141 gctacaagat ggtcgacacg ttgatgtttt accgtatagc tgggagtcga gtgaatttgt
     1201 actcgatgat cggtcgaaag ttgtgacagc caaggcgacg ggaacattta cgcaataccc
     1261 attaaaactc gcctgggcgg tgactattca taagagtcaa ggtaaaacat ttgatcaggt
     1321 tgtgattgat ttaggccgag gaacatttgc tcatggtcag ctctatgttg cactaagccg
     1381 atgtacaaca cttgagggga ttgttttaaa acagttggtg acacgccgcc atatagtaat
     1441 ggatcaacga gtagtggcct ttatgcagca atttaaagag acatctgtat ttcggggtga
     1501 agatgcctac gagccagcgc cacagctatt ttagctaccg agttgaacca atctttcgaa
     1561 gtgttctttt gagacaggct gaactgatag ccgcgctccc ttctggatga ccattaaccc
     1621 agcaaggtct gggtttgctt tcatcgcatc aagcgagagg gggtgggtga attttttaac
     1681 aaaggcaatg tctaccatat accaggttgg atttgacggg tctgatttag gatcaaagtg
     1741 ggggtcctcc ttgtcccaag cggtgaagtc cgggtagcct tcttttgata cttcagccac
     1801 gcctacagcg gcaggtggtt ccgtattaga atggtagaaa ataattttgt ctcctacctt
     1861 catactgtct cgaagaaaat tgcgagcctg ataattacgt ataccatccc agtaggttgt
     1921 tttgtctctc tctaaatcat caatcgaata aaccgaaggt tcacttttaa taagccaata
     1981 ctgcatgagt ggtcctttgt tgttcacctg tacgttcgcg agacttaatt ctggtttatt
     2041 atacgttttt tacattcaaa catgaagctg ttaaaattgc tccatggaca aaggcatact
     2101 cagtattatt ggtgttacga ttattgcggt tggcattgtt tttgccgtgg cagtgaacga
     2161 ttcaagcaaa gaagcaagta tagcgcctcg tgataaatgt gttgaacatg caggtcttgg
     2221 gatgcatatt catcctgaac tggagattcg tcttgagggg caaactgtcg cgctccctgc
     2281 taatatcggg gttacttcaa gttgtatgca tccacttcat acgcacgacg attcaggtaa
     2341 ggttcacgtt gagtatcctc aaaaacgaga ttttcagctg ggtgatttct tcgcagtttg
     2401 gaaccaaccg ttctcgaaaa gtgaaatact cgataaaaaa gttgatgaga cgcatactct
     2461 cactgttacg gtagatggag ctttgaatga agagtacgaa aagcttgttc ttaaagatgg
     2521 acaaaagatc gtcattgatt accacgcaaa gtaaatctct ctaaagccag cttaaaaagg
     2581 aagagaagat ttggtagctt ctctaaggat gtatttcttt ttttagcgta tctttacact
     2641 atgtctattt ctctaataga attgcttgct gcgatacttg tgccgcctct tgcggtgtgg
     2701 ttaaaagttg ggctaacgac ccacttctgg attaacgtta ttttgacgct ccttggctat
     2761 ctgcctggga taattcatgc tttttgggtc atactaacat tttagatgat tcttttattg
     2821 cctagttcct agcctacgca ttaggttttt tgatacccta aagtaggagg tcgtgatggc
     2881 tcacaaaacg tatgaagaac tagcagaagt tttatcttta gcacaggggc aagtcgaagt
     2941 tggtgcgaaa taccagcatt acatgagtga tgaaaaattc tatacggtta tggcagtggc
     3001 tttacaggaa gaaactgaag ctccttgtgt cgtatatcaa acctctcatt cgagtgggct
     3061 catttgggtt cgaaatcttg atgactggtt aagcacggtg gtaaaagacg gtaaatcagt
     3121 cccccgcttt cgtaggatag aatgataggc gtttaatgac tatgatggtg agcttctgct
     3181 gggaacttct ctggcttgcg gataataagc tgggcggtga tataacttgc aagtggaacc
     3241 acgaggataa gtccgatggt gccaataatc gttctcatta tttcggttgc aatttgttct
     3301 tgttgcaacg caacaaaggc cggtaattcg ctttgctgaa ttaagatgag aagcggtagc
     3361 gatacgcctg catatgctaa aaagagcgta ttaataacgg aagatatgtg atcctggccg
     3421 atcttcattg cgcgatgata gagactgctg gcactcaggg atggatttga gtggtagagt
     3481 tcgttaataa tcgaaatttg ggttaaggct aggtcgtcga tgacgccgag ggtaccaagc
     3541 aggatggcgc tcagaagtac ggattgaaga tttatgtccc ggcttaagta aagcgcttct
     3601 tctgtccccg ttccgatgag ctgcagaaga tgactgaacc cgagggagag caacccaatg
     3661 actacggtca tggcaaatat gctcccaagc gcaatatgcg acttcatttt atagccatgt
     3721 gaaatataga gagacggaac aataataatg atccctccca aaattgtgac catgagcgga
     3781 gagtaacctt taacgatcaa cggcacgata gcaaaaagta tgaccgctaa tccgataatc
     3841 attgaaagaa tcgcccgtag tccgtggatg cgcgtgacag cccacgttac gatacaaaag
     3901 agaatcacga gaaggatcag gcctgtactt ctctgttggc ttacgatgct gcaattctct
     3961 tgcttatcta taagtgtgca ggtcgcaatt aatgtatcac cagaatgtac atcaaaatta
     4021 cgtgttgttt ggtcattatt tgaccacagg gcatcaataa attttccttt gtcctttcct
     4081 cctgtcactt gcatagagat gagaattgcg tcacttgatt cgagggtctc tagttctgta
     4141 gaaggggtgt agggacttac gtttgtcact tttacagaat aaaaaacgga gctaggaaca
     4201 gtctctcctt gcgcatggct cagagagaag ggagtgagca acgaaactac aaagaggatg
     4261 gaataaatga agcgtttgag catgacctca tattacatga atcaggaggg taactccacg
     4321 gcgacaatta caatcctggg gcaattgaaa aagcaactaa gttgcgttat aatactacgt
     4381 aatgacatct gctcacacaa ttcttgaatc tcttaagctc actggcgccc gcatgacacg
     4441 tctgcgtgta gccttggtgg ggcttttttg tgacgatatt acaccagtag attacggtgt
     4501 gattgccaac tatttactga gtcagggaat catcgtgaat aaaacgacca tttatcgaga
     4561 acttgagtat cttctcgaac acaacgtcat ccaggaagtg gacttgggtg agagaaagaa
     4621 gcgatacgaa ctggttggtc atcatcacca tcaccttatt tgcaccgggt gtgaggcagt
     4681 agaagcggtg catcttgatg atcacctgca cgatcatgaa ttacgtattt taaaagagaa
     4741 gaaatttaaa gtcacgcgtc atatgctcga atttttcggc ctctgtggta tgtgccaaaa
     4801 gaaagggagc taatatgtct tcgacagcat atgccttcgt gagcgttggg atcgtcagcc
     4861 ttatttcgtt tgtgggagcc gttggacttg tgcttactcg aaagtcactt cagtcctcga
     4921 tgttcttact cgtcagtttt gcggttgggg ttttatttgg tgacgtgttc atccatcttc
     4981 tccccgaatc attcgaagct tttggtgact cattagctac atcttacttt gtattgatcg
     5041 gtattttact tttctttatg cttgaaaagt ttattcgatg gcatcattgc catcatgggg
     5101 aagatgatca cacccatcgc catcacagtt tagcaacgat gaatttagtc ggtgacgggt
     5161 tgcataactt tattgatgga cttttaatcg gtgcttctta tttagtcagt attcccgtgg
     5221 gggttgccac aacggtggcg gtgattgccc atgaaattcc aaccgaaatt ggcgactttg
     5281 gagtgctttt gcatagtggg atgtctgcac gcaaagcatt gctttttaat ttttactctg
     5341 ccctaacggc cgtcgtggga gtggctgttg tcttgcttct cggaaattcg gttgaaagct
     5401 tctcggcgta cttaattcct ttaacagctg gggcattcct ctatatagca gggtcagatt
     5461 tgattcctga gttacatcac cataaccctc aagtaaaaca tagcatcggc cagcttctgg
     5521 ctattattgc tggtattacg gtgatggttc tcctcgcggt ttttgaatag tttcacatta
     5581 aaaaagttgc tgtaagtatc gccactattt cttgatatct cggataatca aggtggtgat
     5641 aattggtagt ccggtaataa caaaaaacca ccagaccgtt agcagccagc ctgattggag
     5701 atgttggccc aggtgaatac tatgccaaaa gacaagaaaa aaggtaagat agtttacata
     5761 ttcgagtggc ttcgcgaatt tttgtatcca tggggtgtta ccaatccatc cagtaatctc
     5821 ggttattaca aaaaccaaaa aggcaatgac agcgagatta atgtagccag taaggttact
     5881 tgggacatag tcgtagaagc tttgaagcgg tagtccaagg tgattattaa attgagcgat
     5941 agccaataag agcggatggg caatcacaaa cgtcataaaa gcatggctcg accatttaaa
     6001 aaatggtctt agggacaagt gtgtcaggta aggtttgcac agtagcccga gcaactgcag
     6061 ccagaatatg gagaaagcaa gtaatccaaa tactggaaaa agttcgtaaa ggcttacttg
     6121 agataaagga cgaggcagtg agcttcccca taccagaatg gggaggagta taacaagtgt
     6181 cgctaaacaa ctcgccaaga acttttggtt atgtgctttc atgaagagtt ttatttcgtt
     6241 tctttttttc gaaaggcaag tgaagctgaa ttaatgcagt agcgttgacc agtcggggtc
     6301 tgaggcgcgt cgtcaaagag gtggcctagg tggctaccac agcgagcgca gacgacttcg
     6361 gttcggcgca tgccatacgc atcgtcatga tgaagttcga tcttttcgct atcaattgat
     6421 tcccaaaagc ttggccagcc ggagcctgaa tcaaatttgg tatctgaaga gaaaagttcg
     6481 ttaccgcagg cgccacatat gtacattcct acttctttat tgttgaggag ttcgccacta
     6541 aaggctggtt ccgttccttt ttcgcgtagt actcgatatt gttcaggggt tagttctgat
     6601 ttccaggtgt cattatcttt ttttattttt tccatatgat cctttttcac cagtatagca
     6661 aatccccact ttaaatcgtg gggattgcca atattctatg gggtctattc tcggcgtatg
     6721 aattagccag atatgctgtg cttgtcgttg tacttttggt gacgggcttc ggctttccaa
     6781 aaaggaccag cggcgacaat ctctgtcata ataggttggc tatacgtgcc caaggcttct
     6841 ctctcggctt tggatttttc agccgccgcc ttttggaggg gtgagtggta aaaaattact
     6901 gaccggtatt ccgatccgat attaggacct tgtcgatttg tggtcgttgg gtcgtggaga
     6961 tcccagaagg ttgtgaggag tgtttcgtag gttacgacat cggggtcgta gctgagttgc
     7021 acagcttgag catggccaga agtgtctgaa gagacttctt cgtaggttgg gtgttccatc
     7081 gttccgccgg tatagccagt ggtcgagtat tttacaccct taactttgtc gaaggtctcc
     7141 tgtacacccc aaaagcaacc ggcggcaaag gttgccatag atatatgtgt ttttcccata
     7201 ttgctattat agcatcctat acaagtgtgt tagaattgtg tcatggaaca attcgaaatc
     7261 aaacatcacg tacgagaagt attaaatcaa gaaattctca tttcactagc tacaacggat
     7321 atgcgacatc aaccctgggc ctccgtcgtt cattttacgg ctaatgatga ttttacgatc
     7381 tactggcttt ctgaccccaa agcacgtcat tcggagaatg tggaaaccac aaagcgggcg
     7441 gccggaactg tcacgttacc gcttgatgca gacgggaaat ctatagcatt acagcttgag
     7501 ggcacagctc gacgtatcgt cggggatgaa gtgaatatgg ttcgtgatct gacggagcgc
     7561 agacggggta agccaatcaa tcctgctctg ttagagacgc ggacttttta cgctcttgtt
     7621 cctgatcgaa tttatcttct ccatgagcct ctcttaggtt atgagcgcgt agaattattg
     7681 ttgtctgctt gacggagttt ataagagtaa gtaaaatgta tgtactatga ataaacggtt
     7741 acacaaaaga aatgattaca cttttgggta cgtctaactg ccggtcatct cgcggcagtt
     7801 ttttctttca tgaggagatt ttatggaaca gatgagcaaa gatacattgt tacaagaatc
     7861 gcgcacccac gttcagtcgg tgcaaaaggc aattactcaa gaaattgccg atatggaaca
     7921 aaaggtcgta agtggtgagg aggagtggcg cagtcagtca gcggatgacc gtgttatttt
     7981 tgatacattc ttagctcact accaagagcg caaaggtgag ttagggcaac tttacccttc
     8041 cccttatttt gtgcggtgtg atgttctgtt tgaaggggaa tcagagatta aaacttacta
     8101 ctttgcaaag tttcccttta gtgaagagca gatttattcg tggacggttc ctgcagcgac
     8161 actacgctac gaggagccgg gagaggtcaa ttacacggct ctcgatggaa ccgtctatca
     8221 tggtaccctt gtgcgcaaag atcaatacat gattgttgat ggtaacgtcg tcttttttgc
     8281 gagtgagacg gccggccggc cacgagaatt gatatatcag gaacatttct ccactcgtaa
     8341 aagtggtttt gtcttaccag aaattgtggc gcaaatggaa aaagcccagg accaggtagt
     8401 tcgcgcggca catcaaggcc cgtttgttat ttccggtcct gctggatcgg gtaaaaccac
     8461 actggccttc catcgtgttg cctatttgat gcaatcgcca gatacggctt ctctctatcc
     8521 ttcagattca attcttattt tggtccaaga tactggaacg cgagactact ttacgcacct
     8581 tttgccggag ctaggaatta agcaagtcgc cattactacg ttctttgagt gggcggtagg
     8641 tgttttagag ttaacgggtt ttgaagcaac gaagtggtct gatttacccg aacccagccg
     8701 tgacagctat gaatatgcca aactcaaagc attgcgagag tttgtggccc ggccaaaaaa
     8761 aggcactgtc tattcatggc tcgaagaagc ctatgcttca tgctttttcc ctcatcatga
     8821 gatattatgg caacagcaaa tcaagagtcg taagcttgat cgaattgata ttacgcttct
     8881 tcttatggct caaaatgcgg aagctggcgg attaactcac atggttgaga cattcgtaaa
     8941 gcagagaaac ggcacgtata aacgaaagtt tgaacgccaa ccaattcgat ataatctcat
     9001 ggttgttgac gaatttcaga actacttacc tgagcagtta acactattgc aggggtgtcg
     9061 aaatcctcgg ttagagtcga caatttatgt tggtgacatg gcgcaacagg ttcaattagg
     9121 gacggtaaaa aactgggagg aagcccggga aaaaattgag acagatcgga tgattgtctt
     9181 ggcaaaggta taccgtaata cgcagcgcat acttgagttc atccgatcgc ttgggtatga
     9241 ggtacatatt cccgagaaga ttcgaatcgg ggatcctgtg gctgaggtgc ttgtcgagtc
     9301 ggttgaggaa gaagttgcat atatcgaaga tcttctaact cgtgatactc aaggaagtct
     9361 tggggttatt gcaaaagaca gcgcatactt ggcgccattt cgaactgctt ttcggggtcg
     9421 agatcgcgta catattgtca ctatgcaaga atctcaagga gtcgaatttg atagggtttg
     9481 ccttgttggg attacgcctg agttattaac ttcccatgtt tttgaggaag ctatacgaaa
     9541 agagaaacgt catattgata gagatttact gtatgtggct cttactcgcg cgatgtcaga
     9601 aatgcatgta ttgggaaatt gttacttgcg ggaaagcatc caatttttag attcttctgt
     9661 ggtaacatga agaagtcatg catatagatt tggacccaag gagatatcgt aattggccat
     9721 ataagaatac aacgcttctc attttaagtt ttcttatact ttggttgatt atcgacactc
     9781 atcctgtaca aagagcagtc tctcaagttt cgggatgggg gtatctgggc gcatttttaa
     9841 caggaatatt ttttgtctct gttttcactg ctgcacccgc ggcagttatc ctctttcgtt
     9901 tagccgatgt cctgaacccc tgggaagtgg cactttttgc tggcattggt gctatgtgtg
     9961 gcgattacct tatatttcgt tacctccagg ataaagtatt cgatgaactg aaaccgattt
    10021 ttttcaaaat tggacgaacg caacttggcc aactctttca taccccttat ttcgcgtggt
    10081 tattaccgat tgttggcgcc gcgattattg cttcgccttt accagacgaa gccgggattg
    10141 ggttattagg cgcttctcag gttcgcggat ggcaattttt gctattaacg tttgtactta
    10201 attccgttgg aatctttttg atagtattat tagcgcagac agcttcttaa acgaaaggaa
    10261 ctcaatgtta ctactttgga ttattaacgc cctcacgctt ttgctcgtag caaactacct
    10321 gcctggcttt cacgtctctg gtttttacgc ggcgctcatt actgcgcttg tactcggtct
    10381 ggtcaacgcg gttattcgtc ccattgtgat tttgtttaca ttaccgatta atttgcttac
    10441 gctcggtttg tttacctttg ttattaatgg ccttatgatc tggttggtat cgactgtagt
    10501 caaagggttt gaagtcgatt cctttaatac ggcgctctgg gcagccttaa ttctttggct
    10561 catcagcttt gtcaccaacg ccttaactaa aaaagcataa aaaacagcgc ccgacctcat
    10621 ggccgggcgc tttcgttcag gatgactagt ttttggcggt agccaaagac tttttgaacg
    10681 tgacgacaaa gagaagaata caagctccta ctacaaggag catccccgaa gccctccatg
    10741 cgacagggat ggtatacgct tcagcgattc gcgcactgaa ggcgagtccg agcatgctcc
    10801 cgagatgact gggcatgctc gtgagtgata ggaccgtggc ccgatgatct tcttgatctt
    10861 tggggatctg gtcgttggcg tatgcatcta tcatcggacg gaatacccct ctccccatct
    10921 gattgaagag gaaggccgtc atcatgagcg cagggcttgc gagatagggt gtcgtactga
    10981 tgccgacacc aaccacgagg agactacaaa ggagtgtata ccttcgatca aaacgagttg
    11041 acttaaggcg tacacataga gcccctcctg aggctaaagc aagtgagata ccaatccata
    11101 ccactcccat catggcctgg gggaagccgt actgtccttt catgagcggt tgccattgca
    11161 tgttgggtgc ctgcgtagca accgcgaaga gcagtgccat acagacaata agccgtacat
    11221 cgctgcttga tactgcgtaa cggacagccc tttgcatggc acgtccgaga tgttgcaggt
    11281 gatcccagat ctcacccctt aggttttgat gcgatacatg gtgatcctgc atgagaacca
    11341 gtgctatcac tgcgttacac cccatcaaac ttcctccgaa cacccaagga agaactaaga
    11401 aatggtgctc gatccaggat gccagtacac cttttccaag tgtatcgaca acagaccgaa
    11461 taacgttgtg gtcaagcgaa agagcgccaa tttgagccgt cgcaaagccg gtaacacagt
    11521 taaggatgta gctcttcccg gtgatgtcgt cgagttctcc agtgtagcca tggtgggtca
    11581 ggctgtgacg aagccacgcc tgaaatgcac cactcgctaa gcatgagccg atcccggcga
    11641 gtacttctgc cataataaac cctgggacgc ctgacgcggt cgcgtacgtg aagtaagcgc
    11701 aagcaatgac gatgcgcgaa atcacgtaac accgcttccg tccaaagacg tcagcgaggg
    11761 cgccggttgg catctcgagg gtgaggcaga tcgtgaagta cacgaagttg accatttgcg
    11821 cttggcccag actgaggccc ttggccatca ggtaggtcgt gtagatcggt gcaaagaaac
    11881 ctgtggctgc gttggtgacg acagcgagca accagtacgt gtgaatgaca cgtcttacca
    11941 tagttttcct ctctcagtga ttcagttgtg aaggttctag aaaagcctaa aaacgcctcg
    12001 gcttttctag ccgaggcgtt tttaattggt aaaaagttac ttgttacaca aaaacactac
    12061 ggctaaaaaa gtcggtgtat acccaaagac cggcctgatc ggctggctgt gagacgtgat
    12121 tcataataaa gttataagtt gtcatagctt gtttagtata gacgatcttg tttaaggagt
    12181 caagcattag agaaatgctc ggtcacgtag tcaaccgtaa agttcatcgc gccgcccatt
    12241 tcgccacttg tttccatgag aattacgtcg tgatggggct ggagaatgtg cttgaagaaa
    12301 ggctccagct cttcttttgt agtgaatgcg cggacttttt tacctgtcga cgcaatttgg
    12361 tcgacgattt cttgctgggt taattgatcg tgagtaccgg ccccatgagt cggtggttga
    12421 aacacaaaga cggtatcggc ttggtcgaaa agagtcaggt attgaggcag aatggctcgg
    12481 tttcgaaaac taaatgtatg tggctcaaac agcacacgaa tccttttatg ggggaattgt
    12541 gcgcggatag cggttaatcc ggctaatagc tttgcaggtg agcttccata gtcttcgtaa
    12601 accgggatgg aagttttttt tgtgcgtaat tcaagtcggc ggcgaatgcc ggaaaatgag
    12661 gcaaaacttt cttcaagtga ttctgcggta actgcattat tgctaagcag ccaagcggcg
    12721 acgcccacca tatttaatac gttgtggtcg cctaagagcc gtgaacttaa gcgggcgatt
    12781 tttttacctt tgtttattaa cgtaaaggag gtgatttcat cacgagtaat atcttcggcc
    12841 caccaatcag ccgctttatt attgaggcta taggttaggg cggttcgttt tactttggct
    12901 agcgtttcgg tgacgtgctc cccatctaaa caggccataa taatggcgtc ttttggaagc
    12961 tttataagta gtttttcaaa aggtgctaga tagctctcga gcgtagggaa aacgtttaaa
    13021 tggtcgtgag tgcaggaagt gagtaagagg gtcgaagcgt tgtagtggag aaatttagaa
    13081 cttgtgtccc aattggcgga agggtattcg tcaccttcca acacaaacat accgcctcca
    13141 cctttatggc caaggtttgg caggttagga cacagagcgg gaacaaagta gctcgggtct
    13201 ttctctgcgc ttgaaagggc ccaggccgta agggcggtcg tggttgattt accaaaacta
    13261 ccggcaacga caaagttttt ggtgtgtgcg gttaattctt cgatgacatc aggataagac
    13321 ttgattggtt tgccgctggc gaaagctgct ttgacctctt cgttctcatc gggaacgagt
    13381 tttgcgtgtt tgccaataat aatagtgtcg acgtcagcgg gaatattact ggctgcgtag
    13441 gggctcagca tagtaacagg gcgcgtttct aaataagtgc taatgggcgg ataaaatcct
    13501 tcgtctgagc cggtgacctc ccagccatga tctagaagaa gatcggccac cgccgccatc
    13561 cctgtaccgc aaataccaat aatgtgtgct ttcatactgc tactgtagca gaggaggtgt
    13621 tactctgtcg attctttgtt ttcgattttc ttaatgatga gtgggatctt ggcaaagtcg
    13681 tctaagagag tttgacgcag acctccgtta taaagagcag cggctgggtg gtagagcggc
    13741 aagtagatgc gcccgtcctt acgacggaat ggcttgccat gcagtttact aatggtgccg
    13801 acccctggta aaagtttgct catggaatga cgaccgagca tgacaataat ctttgggtta
    13861 ataatttcga tttgcttgat taaaagcggc caatgttttt caatctcttc gggggtgggg
    13921 tctcggtttt cgggcggtcg ttgcttgacg atgttggtaa taaaaatgtc ttctcgtttt
    13981 aaaccaatcg actcaagcat ttcgtttaag aatttacccg ctgcgccaac aaatggtacc
    14041 cctagttcat cttctttttt tcctggagct tcgccaacaa agagaatttc ggtatcggga
    14101 tttcctgctc cgggtacgaa attcgctcct tcgtagaggg gaagttgctt gagttcttcc
    14161 aatacttgcg cgagcgctgc gactttattc atgcttgaag tatatcagtt cttctaatcc
    14221 atctcataat ctggcttacg gaaggtgatt ctgttggggg cggcgtcgcc tgctatgagg
    14281 agcgaagcgg gctttaccgt ccagcgacca aacttatggt tcagattctt tgaggcttca
    14341 aagagatcaa ttactttgtg ggatgttgtg tcgaacaaac ttattggctg tggtgtatga
    14401 ggcgtaagac tatgacagac aacgagaatc tgtttggggc aaaaacgatc agataactct
    14461 gtgtcatagg cagctgcaac atgacgaaaa acgtcgaaca cattttgggt gggtgaaagg
    14521 gagagagtgt gttcccatcg gtcggtatgg ctccgcgcca tgatgctgat tctggaagtc
    14581 cccaggtgct tttcgcgtag cctatcggcg actttgatcc agagtttata gagtactgcc
    14641 cgacctttgg cttttgttcg gagtgcggga gctagtacat gagagtggct gatgctttta
    14701 gggcgttctg atgctcggaa cccattcgtg tgttgtaaat tgtatccgtg caagttatac
    14761 caccaggcat cgcccgtgat accaaatgca ttatggagta gatcctgcgg cgcacgaaaa
    14821 aaatcgacgg tagttttgat tcctatagca ttgagccgtc tactcatacc ccagttaatg
    14881 ccggggatct cacgaagagc aagctgagaa aaggcagatt caattgtgtg ctgttggata
    14941 acgactaatc cgttaggttt ttgaatttct gtcccaactt tggccaaaaa catattgggg
    15001 gccactccga cagagcacat gagagctgga ctgaaaatat cagagatacc ttctttaact
    15061 tttatggcga gtgcgtgagc gttgggggtc cattgttcac gggtatccag aggaattcca
    15121 aactcgtcta cgctcagagt ttttacgcca ggtgatacct ctgcgtctaa aaaagagaga
    15181 atgcgattat gaatatcggt gtagtaacgg tggttgccag ggacgacgat taactcgggg
    15241 agtttttgtt tggcttcccc tacccgccaa ccggtcgtga tgccgtgagc cttagcttcg
    15301 taggaggcag caataatgca ggcactcggc ccaagggaag gtgcgacggc aataggtttg
    15361 ccgctcaaag aagggtcatg gtgctgctcg cacgacgcaa agaatgcgtt catatccaga
    15421 aaaagccatt ggttatctcg cggagcaggc aaatggatca taagtccaca ttaccgaaaa
    15481 taagccgaac gagtcaatgg ttgctgttac gaaaaagaga tttcagtccg ccgtttttta
    15541 cgaaaagtaa ttaggattgt gagtgcttcc gattcaacca gctccacgca aaccctagtc
    15601 cgaaaagaat gtggagatag ttatcaatcg tatcaagttg ggttgagaga ttgatcttat
    15661 ttttgataag gattttagca tcaagtggtg cgtgtaaggt gaatccccaa atgccccagg
    15721 cgaggtaaaa aacaatcagg aggccgagtg taatagttct gcccctaggt ttaaggttac
    15781 tggccgtgat ggcgattaat ccgcttataa cgtgaaagag gttttcgtta ttggtaaaat
    15841 gaacgctctt ccaatttgga aaaatataac caaagactcc gaggagaaag aggaaggtgc
    15901 cgagaacttt gagtgcttgt ttggtttgca tattgtttgt atacctgttt ggtttttttg
    15961 tgtaaagagc aaaaatgctt gtttcgcctg tgaaaagggt ataaaatagc gctatctcag
    16021 ttccatttct atccactcag ggggatacga tgaatgctgt attagggcgg gcagatgaac
    16081 atatctctca ctggagggat ctccaaacgg ctgaaccgtt gggtggagtc ctggcgattg
    16141 atcgcctgga tggatcttct gctcgcgcat acacaaagct ggaagatcca atatttcttc
    16201 gggtgcataa ccccgatagc agtgagacgg aagaggttct cgttctccat tctgattagt
    16261 gcctgcttgc gggcaggctg acgtttcgaa tgccccgggt gccttctggc accacggggc
    16321 gttgcttttt tatgggtgac tataccttgg caacaagtag tttgtaagtt gagctactga
    16381 ttacgaaacc aggccgaatt tttacgtact ttagcaacgg tgagatctat taaattcttg
    16441 ctaaccataa tggcgttagc gtgacacaaa tagccaacat atgaaactaa ggtaggctta
    16501 acggcttttg gcccttcttt gaccttggcc agcgttcgtt ttgttgctcg tttaaccgtc
    16561 tttcttcgag gtaacgtgta atgcggcaag gcaacatacc caacgaagtc gatgccccac
    16621 gagagctttc gcagtgttgt tttatgaggg tgaatttgta atttcagacg ctctttggag
    16681 aatctattaa tttcaacaaa ataaccaatt aattcgtcag gatcagaact aagtaagatc
    16741 atgtcgtcgg catagcgaag ataatatttg gccttgaggt gatgttttac gaatttatct
    16801 agtggatcga gataaacatt ggcaaaaagt tgtgaggtca agttgccgag cggcataccc
    16861 ttgccctcgt tcatattaaa gctaccaata atattgtcca aaaggtggag taacttacgg
    16921 tcggaaatcc tagaggagag caagttgaaa agcgtaacat gatcaacgct gtcaaagaac
    16981 tttcggatat caaatttgag cgcccagcaa gggccgctgt aattcttact tactttacgc
    17041 gctatctctg ttatccgctc aaaagcttta tgcgttcctt tgccgacgcg acaggaatag
    17101 ctgtcaaaaa tgaaggtgcg gtcgtagagc ggatagagga cgcggtaaat cgcgtgatgc
    17161 aacagccgac actcgaccgg tgctttgctt attaaacatg gctttgggtc gttaatccgg
    17221 aactgttcat accacgtctc tggaaaaatg cctcgtttca ggttaaaatg caaacgaaac
    17281 aaattatctt ctaagtttcg ttcgtaggat tggacgtcag gcttacggcg ctttccccta
    17341 cggaattcta gccaagcctg gtggatattt tcgagcgaaa caatttcagc aaaggaaccc
    17401 ttgtgaacaa tacatctgct ttcaatgtca cccc
//
LOCUS       DASWYB010000027        18489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_433974, whole genome shotgun sequence.
ACCESSION   DASWYB010000027 DASWYB010000000
VERSION     DASWYB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18489
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433974"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VGE59_01410"
     CDS             <1..485
                     /locus_tag="VGE59_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1074342.1"
                     /translation="FLIYRFDLNHQDLGTFFLYTGLWLIFGQLVTVRLVANKLPEEKL
                     VYLSVFGAAGVMLLYLVPTNWYGLMFVLPFFTNFMAISMANIPALVSKVATSDRQGEY
                     MGINNSVQAVALTIAPILSGYISAQLTPVSIIITAASLMACAALIFAFYASLKKSQYY
                     "
     gene            568..1125
                     /locus_tag="VGE59_01415"
     CDS             568..1125
                     /locus_tag="VGE59_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074343.1"
                     /translation="MRRRHRVLWGFSLGVVVTPEGDRLAENTIATIQQVEVFLNNAKE
                     AGRPYTAILFTGGIFEKDKGQTTAVSELMRDAIGPKDIPVYIGTNSAITRQDITDGIE
                     VLAEYGITLENADIDVVSEFWHTLGIAMLFLRRFGYRVGRVSSGYKISFKEIAGRIAR
                     LAYYAYDPEGKGDQATAVATRRCGR"
     gene            complement(1244..2236)
                     /gene="trpS"
                     /locus_tag="VGE59_01420"
     CDS             complement(1244..2236)
                     /gene="trpS"
                     /locus_tag="VGE59_01420"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY1074344.1"
                     /translation="MKPDYKVDIFTGIRPTGTLTVANYLGAVAPVVSFQNEGKEGLVF
                     VADLHALTDAEPATAEKFTYDIVADYIALGLDPNKIKIFAQSAISNEVNDLMLILARH
                     VTVAELLRIPTLKDKLKGDAQPENANSLLLMYPVMMAADILLQRAKHVPVGDDQMPHL
                     EMTRLLLRRFNSKYAEVFAEPQALKIETLRILSLKGPGKMSKSNPEGAIFLTDSAEMV
                     ADKIKRAQTAAEGEMSDSLKSHIIVAKSLASSESDRQQIDAIIAEHMSGKPVMGQFKQ
                     LLTKIVQEFLTDFQTKRAQITEADIKAILEEGNKIARENAQETIALVKEALYAK"
     gene            complement(2247..3767)
                     /locus_tag="VGE59_01425"
     CDS             complement(2247..3767)
                     /locus_tag="VGE59_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY1074345.1"
                     /translation="MATLQDYRDERLRKLNELKELGINPYPAKAQRSNVLADITNKFS
                     DLEGKQVSVVGRIANIRKFGTIAFLVIRDASGSLQLFLNKKSTGELDPSCNQLGMEQL
                     PLLDTGDFVEAHGPVIKTQTGEISVEVHSLRLLTKSLRPLPSAIEGFTNKEERFRRRY
                     VDTNVNPDVFARYLRRSSFWQSTRNFLLDEGFIELNIPVLELTTGGADAAPFVTHMDA
                     LDQDFYLRISHELPLKRLLVAGYEKIFDIGPRFRNENYSDEHLPEHVAMEWYWAYADW
                     EQGMALTERLVRTIADKTWQKRQFVLANGMEVDLGPDDMAWPRISFVAIMQETYGINV
                     FTSTLDEVKEQLKKHNLQVEKSENRARGIDKLWKKVRTTIAGPAFLVDIPTFLQPLAK
                     TQPDNPELSQQFNILLGGSELCKAYSELNDPLDQLNRFKEQQAMREAGDDEAMMLDID
                     YVEALEYGMPPACGFGYSERLFWVLEGVTAREGVIFPQLRREVDEVTKKIYPDLKL"
     gene            3935..5218
                     /gene="serS"
                     /locus_tag="VGE59_01430"
     CDS             3935..5218
                     /gene="serS"
                     /locus_tag="VGE59_01430"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY1074346.1"
                     /translation="MLDIKIIREEPQKVRDAFISKGHPASLVDDILAADEKLQPLRRK
                     VEDLRAERNKIAAMGPHAAEQGKKIKQELEGISNELTEATEHLDELLLSAPNLPSSDT
                     PVGKDDSENVVVREWGTKPHFDFTPKPHWELGEELDIIDNQRAAKVSGARFTYLKGRL
                     ALLQFALIQLAMQVVTDQDTLQKIIADHKLSVPAKPFTPVVPPVLIRPEAFQRMARLE
                     PKDERYYIPSDDVYLIGSAEHTLGAMHMDEVILESELPIRYVGYSTAFRREAGSYGKD
                     TKGILRLHQFDKVELEAFTTPEQGLEEQNFFVAIQEYLMQQLNIPYQVLLCCTGDQGD
                     PDARHLDINSWMPGQDTYRETHSSDYMTDYQARRLKTRVKRTATENQYVHTNDATAFA
                     IGRTLIAVMENYQQADGSIKVPEVLVPYCGFSEIR"
     gene            5266..5342
                     /locus_tag="VGE59_01435"
     tRNA            5266..5342
                     /locus_tag="VGE59_01435"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5300..5302,aa:Met,seq:cat)
     gene            5434..5508
                     /locus_tag="VGE59_01440"
     tRNA            5434..5508
                     /locus_tag="VGE59_01440"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5466..5468,aa:Met,seq:cat)
     gene            complement(5547..5960)
                     /locus_tag="VGE59_01445"
     CDS             complement(5547..5960)
                     /locus_tag="VGE59_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY1074347.1"
                     /translation="MASPKKTDFKSNPLGVTKTVVWAVIIGYSLFLVGRSTYQNYSMN
                     REISVQKKRVAELQRVKSLQELALIYYKSNAYREVEARRRLNLKGKDEFVVALPQAST
                     EPRLTIASVASGDGGETALSPFQAWWNLLFVSEEE"
     gene            complement(6024..8390)
                     /locus_tag="VGE59_01450"
     CDS             complement(6024..8390)
                     /locus_tag="VGE59_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY1074348.1"
                     /translation="MSELRQRFKRLISQVLIGLVSFMQFAVFMPAQLAHAAVSSTLVI
                     SQVQTNGTTATDEFVELYNKTNSPISLNGWSLQYKGGGSAQFPTAVTSKLNFAVGDSV
                     PAHGYFLIVNASASAPLLLQKDTTYADSAISLSNSGATLFLVNSTTALTDGNSSAIID
                     KVAYGAASGSGALYAEGTATAAPAANGSIVRQPNSTLGNGTDTDVNSADFSVQVTSTP
                     RSSLSDTRPVSSPVLTVDSAKDGKVKVSWPLVIGADGYEYAITPTGQAFGVNTAVSAV
                     VLEHEFTGLTDNTSYDIRVRAMRSGTGVGGVDEYSEYALISATPTPSVVVTPDLLKAS
                     VVYSRNGSSVTPFMEGVITVQATVTSGSVPAGDDLLLTLTRPGFATQVVTLTKTGGSV
                     WSGSYVIQDGSQVTDETISASLKTESGNGVEVTSGATFTVNTNVSNPVVTVMSRCSTA
                     QDSLTAKADSDVTLVYVSTSPNFTLANLEGLVLVAPVTSGSTTAEAFIGDNLFGTLYA
                     YAKDAAGNVSGATQFVNDIVAPSVPTVQIEAGDRKLVVSWAAIADAKEYVFRYKKSGT
                     SVWTEQVVTSNVQTLAVENGQGYDIAVASRDAVCNTSAFISNYAMAVAPQVIATSGRG
                     GISEHEAEILARYAVVVEEKDGNPLDFKSPYSVDEDKDQNGIKDSEEDKDGNGIKDGE
                     DKDGNGIKDSDEDKNGNGIKDSEEQPSTNVRDRSRLIVAIAILLIIAGAAIAAYSWAQ
                     SDNDTTGGAAPAPAPEKKDADEKAETVPAEEPQRKTKRGGKSKRKTRW"
     gene            complement(8716..8791)
                     /locus_tag="VGE59_01455"
     tRNA            complement(8716..8791)
                     /locus_tag="VGE59_01455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8756..8758),aa:Ile,seq:tat)
     gene            complement(9049..9642)
                     /locus_tag="VGE59_01460"
     CDS             complement(9049..9642)
                     /locus_tag="VGE59_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY1074349.1"
                     /translation="MYTEKTFSVMEKEIQGISKQTMEGHYKLYQGYVKKANEILEKLE
                     TVDRDPAKANQTYSDIRELKVEFSFAVGGIKNHEIYFSHLGGDGSAPTGKLAEMIDRD
                     FGSFEAFKQDMKATGIAGRGWVWLAYDHDSGKLFNYIGDTQNTYPIWNATPLVALDVY
                     EHAYWGDYGPARGDYIDVFFANLDWRVIEKSAERILS"
     gene            complement(9704..10255)
                     /locus_tag="VGE59_01465"
     CDS             complement(9704..10255)
                     /locus_tag="VGE59_01465"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY1074350.1"
                     /translation="MKIYTKTGDKGTTGLVDGSRIRKSSLRIEVLGTFDEANAHLGLL
                     ITFLSSDHTSLDLLLKAQSMLFSCGARIAEPSNQSHLKLTLPTADFIGEFEKSIDVMT
                     QDLPPLTQFILPGGGVLSAQVHIARAVIRRCERLLVALEDEGSIVEPEVLQWVNRLSD
                     WLFTLARFYNQEKNIVERVWEQG"
     gene            complement(10255..11229)
                     /locus_tag="VGE59_01470"
     CDS             complement(10255..11229)
                     /locus_tag="VGE59_01470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY1074351.1"
                     /translation="MEPKPKVVVTWPISDKALALLREKYEVAVNEKEEALSPEELLGF
                     VKGAHAILCLLTNKITGEVCDAAGSQLKIVATMSVGFDHIDLAATAQRGIVVTNTPGT
                     SDNAVAEHTITLMLSLAKHIPLADKFVRQGKYSGWDPKIFNGPELSGKVLSIVGLGHI
                     GSRVAHIAGFGLGMAIAYTDIRPNPDFESKFNAKYFSVDEVFSVGDVVTVHVPLLPAT
                     KHLVNAARLSTMKPTAYLVNTSRGPVVDEAALTDALKRKLIAGAAIDVYENEPNITPD
                     LLSLENVIMTPHTASATFEAREAMAQKAVANILAVLSGQPALDLVQAK"
     gene            complement(11243..12094)
                     /locus_tag="VGE59_01475"
     CDS             complement(11243..12094)
                     /locus_tag="VGE59_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY1074352.1"
                     /translation="MLVTLNPVLRAAQHHKFAVGAFNVYNLESVEAVISAAEEMHAPV
                     VVQTSEKAITYAGMSELAAIVLTRAKASFVPVVFHVDHGRTHRVLYDAIEAGYSSIMF
                     DGSHLPYEENVRWTKEMVDRAHRKGISVEAELGRVGGVEDDISAEEGLTDPGMAVEFI
                     RATHCDALAVGIGNNHGKPKPGEKLHLDQLEHIAAAVSLPLVLHGASGTPEDDIRQAI
                     ALGITKINIDTDLRIAFSEAEREVLASDPKLYDPRSILAPGTEAMREVVKQKIALFGG
                     HLKAKYC"
     gene            complement(12094..12453)
                     /locus_tag="VGE59_01480"
     CDS             complement(12094..12453)
                     /locus_tag="VGE59_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074353.1"
                     /translation="MQNKYPLGMVLLWLVAAAWLGAQVAFWVGINIHPVLFFSLMIVF
                     FVGAYLSPSRLALVLTILFVLSRSIIAAGYFAFSGLVYQGIFIGYLNIVYVLLGGLAL
                     LLIVRRREFLRNLRRSK"
     gene            complement(12453..13436)
                     /locus_tag="VGE59_01485"
     CDS             complement(12453..13436)
                     /locus_tag="VGE59_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1074354.1"
                     /translation="MSNYFVKDYLDEKRIEQAPTREGFGKALVAIGEQDERVVGLTGD
                     LSESTRMDGFQKSFPERFFEVGVAEQNMMGIAAGLALSGKIPFITSYATFSPGRNWDQ
                     LRVSVCYSDANVKVIGCHSGLSVGPDGATHQALEDIAITRVLPNLIVVAPADSNEAYQ
                     ATIALAKYEGPAYMRLTREKSPVFTSSSQPFVVGKAIHALAGDDVTIITAGPISYEVL
                     QALEGLSDRDITCDVIFCPTIKPLDQDTILKSVRRTKAVVTVEEHQIDGGLGSAVCEL
                     LAEHHPVPVERVGMPNSFGQSGQPNELWATYGLTRKSITDAVKRAIKRKSS"
     gene            complement(13429..14271)
                     /locus_tag="VGE59_01490"
     CDS             complement(13429..14271)
                     /locus_tag="VGE59_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY1074355.1"
                     /translation="MTTKQLEMRANEIRKAIIKMLVSAGSGHSAGPLGMADVFTALFF
                     AVMEHRPRNPKWEGRDRFILSCGHIAPVYYASLAYAGYFPIRELSSLRKFGTRLHGHP
                     HNLETPGVETSSGPLGQGLSQAIGMALAARLNKERHRVYCVLSDGEQQEGQIWEAVMF
                     AGNYKLNNLTAIMDRNNIQIDGPTESVMALEPIKEKYEAFGWHVIEVDGHNMRQIIDA
                     CDQAKAIVEKPTLILAHTIPGKGVDFMEYDYHWHGKPPKPEEAAIALKELRTLGGKIR
                     SEHE"
     gene            complement(14252..14902)
                     /locus_tag="VGE59_01495"
     CDS             complement(14252..14902)
                     /locus_tag="VGE59_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074356.1"
                     /translation="MIIPALLTTNKRIATERILLAREMSGWIHVDYIDHSLYKFESLS
                     LEDLSSLDFGEMAVEVHCMTDFPLKVLESDLPIDRLIIHVESKNWEKDYISAVNKGVD
                     TWLAIDPFTPLASLQLPPDLSGVVMMGIEPGQMGQEFISATYDRLLFISEHYPDCSVT
                     VDGGVGKENIRQLIAHGADNLVMGSAIFHQTHPVQAFRMYEHLSDPIGGAYDYQAA"
     gene            complement(14899..15522)
                     /locus_tag="VGE59_01500"
     CDS             complement(14899..15522)
                     /locus_tag="VGE59_01500"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074357.1"
                     /translation="MAYQYKIGVSGSAVNNCAPGAYAKSREIGRQIALAGAVCVTGDT
                     VGIPHAAAHGAKAAGGITVGISPATSRTEHERKYRLPKDEMDIIMYTGFQYSGRNLLF
                     IRSCDAIIFICGRIGTLNEFTIAFEDRKPIGILTGTGGVADELEHIMEVSQRGKSKVA
                     IDDNPERLVRKVMELIEMDECMRELKQKKTCEIRLGKRQVHQAKALK"
     gene            complement(15545..15982)
                     /gene="rpiB"
                     /locus_tag="VGE59_01505"
     CDS             complement(15545..15982)
                     /gene="rpiB"
                     /locus_tag="VGE59_01505"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY1074358.1"
                     /translation="MYTIFIGSDHAGYSLKSFLIQELTSKYDVIDCGPERLDKQDDYP
                     DYAQKVCEQVLEHSARGILICDTGIGMSIAANRYEGIRAALVTTEFMAERSRLHNNSN
                     VLCLGEALLSNEENLALVHTWLQTDFIEEERHIRRVEKLDMLG"
     gene            complement(16075..16743)
                     /locus_tag="VGE59_01510"
     CDS             complement(16075..16743)
                     /locus_tag="VGE59_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074359.1"
                     /translation="MTTGHDFWKIVGELSPGDWCLSRGAHEAATACLEGAVPGCSQWR
                     VHESPEEDNALYYLLVDIATKQEKLPPFRVEPANVRFYYDLDRFQKRLQEKPIMARTT
                     FVWYMPDTSDSHLAVVPLQSRIVQVVHREMWYAGEPEAPRSGRYSTFYTLAVCDIDDT
                     YLDVYAAPPGWDQRILRVRDWLRHAGRFEKAQIDLDPTLAGLVQLHGIEAVDRALEPY
                     RTAK"
     gene            complement(16854..>18489)
                     /gene="mutL"
                     /locus_tag="VGE59_01515"
     CDS             complement(16854..>18489)
                     /gene="mutL"
                     /locus_tag="VGE59_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY1074360.1"
                     /translation="EISLDDGGKTFIEMKDNGSGMDREDAVSCFLRHGTSKIKEVDDL
                     LHVTTLGFRGEALAAIGASAVVEIVTGKADSSEATQVVMEHGVLSSPKPHPLVKGTRI
                     QVRDLFSALPARQKFLKSEATEWKATLETLVKQMIVHPEVAFMVKHNGKTIYDLPSHD
                     FSSRVAQVWKVEPQALISVDREVPHMALSGVVAQPMIAHDGKARQFFAVNGHPVNDKM
                     VARAIKDAFGTLLPPSMYPSYALNLVIHPGMVDVNIHPRKDEVRFVNSQEVFRFVMQS
                     VSQSLAGVNNAFTSLPTFSVPSAPYASPSTQLESMPFERIERGGFLESGTSFGGGTQR
                     SSFSSSPFQAMRQFVNEPTEIQSSPIITIDNCFLVAQHERKLLLVDQHAAHERILYNQ
                     LWAQEKQKEVVRQALLLPAELSLSPDERALFHVHKSDFEAIGFAFTAGDDVYLLEVPQ
                     VMKLSDPVKFFRSVLGGLTEDRVEPELTDFKHKLFATMACKAAVKAGDVISEGEKQRL
                     IKDLLEAESFTCPHGRPSHIEISGTDLEKLFKRTGF"
BASE COUNT         4995 a         4830 c         3934 g         4730 t
ORIGIN      
        1 tcttcctaat ctaccgcttt gaccttaacc atcaagacct tggaactttc tttctctaca
       61 ccggcctctg gctcatattt ggccagcttg tgacagtacg gctagtcgcc aataaattac
      121 ctgaagaaaa gctcgtatac ctaagcgtat ttggggcagc aggcgtcatg ctgctttacc
      181 ttgttcctac caactggtat ggcctcatgt ttgttctgcc tttctttact aattttatgg
      241 ccatcagcat ggcgaatatt cctgccttgg taagtaaagt ggcgaccagt gatcggcagg
      301 gagaatacat gggcatcaac aacagcgtcc aagcagtagc acttactatc gcgcccattc
      361 tttctggcta tatatccgct cagttaactc ccgtcagcat cattattacc gccgcaagct
      421 taatggcatg cgccgcgctt atttttgcat tttatgcttc tctaaaaaaa tctcaatact
      481 actaacttcg ttgacgaaag tatattttaa catataatac ccccaagctc acaacggtac
      541 tagttctaga gaaaggggga cgatgacatg cgccgacgtc atcgggtact gtggggattt
      601 agtctaggtg tggtggtaac cccagaaggt gatcgcctcg ccgagaacac catcgcaacg
      661 atccagcagg ttgaagtttt tctgaataac gccaaagagg cagggcggcc atatactgcc
      721 atcctcttta ccggcgggat cttcgagaaa gataaaggcc agaccacggc tgtctcggaa
      781 ctcatgcggg atgcgatcgg accgaaagac ataccagtct acatcgggac aaattccgcg
      841 attacccgcc aggatatcac cgacgggata gaggttctcg cagagtatgg catcacgctc
      901 gagaatgccg acatcgacgt agtaagcgaa ttctggcaca cgttgggaat tgctatgctg
      961 ttccttcgtc gatttggcta ccgggtaggc cgagtatcct ccggttacaa gatctccttc
     1021 aaagagatcg ccggccggat cgcccgactc gcttactacg cctacgaccc ggaaggcaag
     1081 ggagaccaag ccactgccgt ggcaacccga cgctgcggaa ggtaatacct ccgttcacac
     1141 cccacccagc aagcatggtg cttctctggg tgggagcccc agagcttact tttcaagtag
     1201 attctggcag caaagaaaac cgcctcactg aggcggtttt cttttatttt gcgtacaggg
     1261 cttcttttac caatgcgatt gtctcttgag cgttctctcg ggcaatttta ttaccttctt
     1321 ccagaatcgc tttaatatct gcttcggtaa tttgagcccg ctttgtttgg aagtcagtca
     1381 aaaactcctg cacaattttc gttaagagct gcttgaactg acccatcacg ggcttaccag
     1441 acatatgctc agcaattatc gcatcaattt gttgcctgtc cgactccgac gaagctaggc
     1501 tcttagctac aataatgtgg ctcttgaggc tatccgacat ttcgccttct gcagctgttt
     1561 gggcacgttt aattttatca gcgaccattt cggcactgtc ggtaagaaaa atcgcgcctt
     1621 cgggattaga cttgctcatt ttacccggcc ccttaagaga taaaatacga agggtttcga
     1681 ttttaagcgc ttggggctca gcaaagactt cggcatattt actattaaat cgacgtaaca
     1741 gtaaacgagt catttccaag tgcggcatct gatcgtcacc tactggaaca tgttttgcac
     1801 gctggagcaa gatatcggcc gccatcataa ccgggtacat caaaagtaat gaattcgcat
     1861 tttccggctg agcatcgcct tttaacttat cttttaaggt tggaatacga agcagctcgg
     1921 ctaccgtaac atggcgagcc aagataagca tcagatcatt tacctcgtta ctaatcgcgc
     1981 tttgcgcaaa aatcttaatt ttattgggat ccaatccaag ggcaatatag tctgccacaa
     2041 tatcataggt aaatttttcc gctgtcgcag gctctgcatc agttaatgcg tgaaggtcag
     2101 ccacaaagac taacccttct ttaccctcgt tctgaaagct caccacggga gcaactgcgc
     2161 caagataatt agcgacggtt aacgtaccgg ttggccggat tcccgtaaat atatcaactt
     2221 tatagtctgg tttcataatt actcatttat agttttaaat caggataaat cttcttggtt
     2281 acttcatcaa cttctctccg tagctgcggg aaaataacgc cttcacgggc agtaactcct
     2341 tcgagaaccc agaatagtcg ctcagagtag ccaaatccgc aggctggagg cataccgtat
     2401 tcgagagcct cgacgtaatc aatatcgagc atcatcgctt cgtcgtcacc cgcttcacgc
     2461 atggcttgtt gctctttaaa tcgatttaac tgatcaagcg ggtcattcag ctcactatac
     2521 gccttgcaga gctcacttcc gccaagcagg atattaaact gttggctaag ttctgggttg
     2581 tcgggctggg tcttggctaa tggctgcagg aaggtaggaa tatccactaa gaaagcagga
     2641 ccggcaatcg ttgtacggac ttttttccac aacttatcaa taccgcgagc acggttttcc
     2701 gacttttcaa cttgtaaatt atgcttcttt agctgttctt ttacttcgtc gagagtcgag
     2761 gtaaagacat taataccata cgtttcttgc atgattgcga caaagcttat acgtggccac
     2821 gccatgtcat cagggcctaa atcaacttcc ataccattgg caagtacaaa ctgccgcttc
     2881 tgccacgttt tgtccgcaat cgtccggact aagcgttcgg tcaaggccat accttgctcc
     2941 caatccgcgt aagcccaata ccattccatc gctacatgtt caggcaagtg ttcatcagaa
     3001 taattttcgt tacggaaacg gggaccaata tcgaagatct tttcgtagcc tgcaacaagc
     3061 aatcgcttga gaggaagttc atgggaaata cgcaagtaga aatcctgatc aagcgcgtcc
     3121 atatgcgtta caaacggcgc cgcgtcggca ccacccgtag taagttctag gaccgggatg
     3181 tttaattcaa taaatccttc atcgagcaaa aagtttcggg tggattgcca aaatgagctg
     3241 cgacgaagat aacgagcaaa cacatctggg ttcacgttcg tatcaacgta gcgacggcga
     3301 aaacgttctt ctttgttcgt aaaaccttcg atagcactcg gtaatggccg aagagacttt
     3361 gtaagcaaac gcaaactatg aacttcaacc gaaatttcac cggtttgcgt ttttattacc
     3421 ggaccatgag cttcgacaaa gtcacccgta tctaagagtg gtagctgctc catacccaat
     3481 tggttacacg atgggtcgag ctcacctgta gattttttat tcaagaaaag ctgaagactg
     3541 ccagaggcgt cccgaatgac aagaaaagcg atcgtcccaa attttcgaat attggcaatg
     3601 cgtccaacca cactgacttg ctttccttct aggtcgctaa atttatttgt aatatctgcc
     3661 aagacattac ttctctgggc tttggccgga tatggattta taccgagctc tttcagttcg
     3721 tttagtttgc gcagacgttc atcgcgataa tcttgaagtg ttgccataca agtgaaacct
     3781 caactctaaa tacgaaaatt gctttgtttt gttactatct gattatactc tataaatcgc
     3841 ctgagacaca aaaaacgtgg ctgctgcaag tattttttgg taaaatcagg atacgaaaag
     3901 aaaatgtttc cctagagagg gacaaaggag ccgtatgctc gatataaaaa tcatccgcga
     3961 agagccccaa aaagttcgtg acgcctttat ttccaagggt caccctgctt cacttgtcga
     4021 tgatattctc gcagctgacg aaaagctcca gcccttacgc cggaaagtcg aagaccttcg
     4081 cgcagagcgg aacaaaattg cagctatggg accgcacgca gccgaacaag gcaagaaaat
     4141 aaaacaagaa ctcgaaggca tcagcaatga gttgacagaa gcgactgagc acctcgacga
     4201 gcttctcctc tcagcgccca atctcccatc ttccgacacg cccgtcggaa aagatgactc
     4261 agaaaatgtc gtcgtacgag aatggggcac caaaccacac ttcgacttta ctcccaagcc
     4321 tcactgggag ctaggagaag aattagatat tatagataac caacgtgctg ccaaagtttc
     4381 tggtgctcga tttacctatc ttaaagggcg gctcgcgtta ctgcaatttg ctttaattca
     4441 gctggcgatg caggtcgtta cagatcaaga tacgttgcag aaaattatcg ctgatcataa
     4501 gctctctgta cccgccaaac cattcactcc ggtcgtcccc ccagttctta ttcggcccga
     4561 agcgtttcag cgcatggctc ggctggaacc gaaagacgaa cgttactaca ttcccagtga
     4621 tgacgtctac ttaataggaa gcgctgaaca cactcttggc gctatgcaca tggacgaagt
     4681 catcctcgaa agcgaacttc ctatccgcta cgtaggatat tccacagcct tccgaagaga
     4741 agcaggatca tacggaaaag acacaaaagg cattttacga cttcatcagt ttgataaagt
     4801 tgagctggaa gcctttacga ctccagaaca aggccttgaa gaacaaaact ttttcgttgc
     4861 tattcaagaa tatctgatgc aacaactcaa cattccgtat caggtgctcc tatgctgcac
     4921 gggcgatcaa ggcgaccccg acgcacgtca cttggatatt aacagttgga tgcccggtca
     4981 agacacatac cgagaaacgc actcgtctga ctacatgacg gactatcagg cacgccgctt
     5041 aaaaacccgt gtcaaacgaa cagccaccga gaatcaatat gtccacacca acgacgcaac
     5101 cgcatttgca atcggccgta cattaattgc tgttatggaa aactaccagc aagccgacgg
     5161 ctcgattaaa gtaccggaag tactcgtgcc ctactgtggc ttttcggaga tcaggtagta
     5221 catacgttta ttgacaatcc atcccgcatg ccatataata attcatgcgg gatggagcag
     5281 cccggtagct cgtcaggctc ataacctgga ggtcgtaggt tcgaatccta ctcccgcacc
     5341 cacatcaaat gacccctaaa gggtcatttt ttgttcctac tctgttgagc aattaccagg
     5401 aatatggtat aaaatagtgt gtacacaccg catggctgcg tagctcagcg gtagagcacc
     5461 ggactcataa gccgatggtc ggtggttcga atccacccgc agccaccaaa aaatccccag
     5521 cacacgctgg ggattttttg tacgctttat tcttcctcag agacgaacag aaggttccac
     5581 caggcctgga agggagaaag agcagtctct ccaccatccc ctgaggcaac tgaagcaatc
     5641 gtaaggcggg gttccgtcga ggcttgaggc aacgccacga caaactcatc tttcccctta
     5701 aggttaagcc ggcgtcgggc ttcgacctct cgataggcat tacttttata ataaataagg
     5761 gccaactctt gcaaagactt tactcgctgt aactcagcaa ctcgtttctt ttgaacagaa
     5821 atctcccgat tcatcgaata gttctgatag gtacttcgac ctactaaaaa caagctatag
     5881 ccaataataa cggcccagac aaccgtcttg gtcactccaa gtgggttcga tttaaaatct
     5941 gtttttttag gcgacgccat gaatccataa taccataaga gcctcgcctt tcagcgaggc
     6001 tcttcaccct ttttaacgca ctcttaccaa cgagtctttc gctttgattt tccaccacgc
     6061 ttagtcttgc gctgtggttc ttcagctggc actgtttcag ccttttcgtc agcatctttc
     6121 ttttctggag caggagcggg agcggcaccg cctgtagtgt cattatcgct ctgagcccag
     6181 gagtaggcag caatagcagc gcctgcaata ataaggagaa ttgcaatggc gacaatcaaa
     6241 cgagaacggt cgcggacatt ggttgaaggc tgttcttcgc tgtctttgat accgttgcca
     6301 tttttgtctt catcgctatc tttgatccca ttaccatctt tatcttcgcc atctttaatt
     6361 ccgttaccgt ctttgtcttc ttcactgtct ttaataccgt tctggtcctt atcttcgtcg
     6421 actgagtaag gagacttaaa gtcaagaggg ttcccatctt tttcttctac cacaacagca
     6481 taacgagcca aaatctcagc ttcatgctca ctaataccgc cgcggccgga agtagcaatg
     6541 acttgaggag ctacggccat agcgtaattc gagataaagg cactcgtgtt acataccgca
     6601 tcgcgagaag caacggcaat gtcataacct tgtccatttt cgacggcaag cgtctgaaca
     6661 ttacttgtta ccacctgttc agtccaaacc gaagtaccgc tcttcttgta acggaagaca
     6721 tactctttgg catctgcgat agctgcccaa gagacaacga gcttacgatc cccagcttcg
     6781 atctgcactg ttggtacgga aggagcaaca atatcgttca caaattgagt tgccccactg
     6841 acgtttcctg ccgcatcttt cgcataggcg tagagggtgc caaacaaatt atcaccaatg
     6901 aacgcttctg ccgtcgtgct tccgcttgtt acaggagcaa caaggactaa cccttctaag
     6961 ttagctaagg tgaagtttgg cgaggtagaa acgtacacca acgtcacatc actatcagct
     7021 ttcgccgtga gtgaatcctg cgcggtcgag catcggctca tgaccgttac gactggatta
     7081 gaaacgttcg tattgaccgt aaacgtcgca ccagacgtga cttcaacacc gttaccactc
     7141 tcagtcttta acgaagcaga aattgtttca tcggtcactt gggagccgtc ttgaataaca
     7201 taggacccag accatacact accgcccgtc ttggtgagcg tgaccacttg ggtcgcaaag
     7261 ccgggccgag taagcgttag cagtaagtca tcacctgcag gaactgagcc tgatgttacc
     7321 gttgcttgga cagtaatgac cccttccatg aaaggcgtaa ccgagctgcc attacgcgag
     7381 taaacaacag aagccttgag gagatcggga gttacaacaa ctgaaggagt tggtgtcgct
     7441 gaaataagcg catactcgct atattcatca accccaccaa cacccgtgcc actacgcatt
     7501 gcacgcaccc gaatatcata acttgtatta tcagtcagac ccgtaaactc gtgttcaagc
     7561 acaaccgccg aaacagccgt gttgacccca aacgcttgcc ctgttggcgt gatagcatac
     7621 tcatacccgt cagcaccaat aactaacggc cagctaactt tcacttttcc atctttagca
     7681 gaatccaccg tgagaaccgg gctcgacact ggtcgggtat cactcagcga cgaacgtggc
     7741 gtacttgtta cttgaaccga aaagtcagcg ctgttgacat cggtatcagt cccattgcca
     7801 agcgtgctgt ttggctgacg aacaatactg ccattggcag cgggggctgc agtcgcagta
     7861 ccttctgcat agagcgcacc cgaaccactc gctgccccat aggcaacttt atcgataatt
     7921 gcggaactat tcccatcggt taaagcagtg gtgctattga ccaaaaataa tgtcgcaccc
     7981 gagttagaca atgagattgc cgaatcagca tacgtcgtgt ccttctgcaa aagaagagga
     8041 gccgaagcgg acgcattcac aatcagaaag taaccgtgag ctgggacact atctcctacg
     8101 gcaaagttta gtttactggt gactgccgtc gggaactgag ccgaccctcc acctttatat
     8161 tggagcgacc aaccgttaag agaaatcggg gaattcgtct tattatataa ttcaacaaat
     8221 tcatcggtcg cagtcgtgcc atttgtctgc acttggctaa taacaagtgt tgaacttact
     8281 gcagcatgtg ccaactgggc cggcatgaac actgcaaact gcataaacga aacaagccca
     8341 atgaggactt ggcttatgag tcgtttgaat cgctgtcgca attcactcat acaatacctc
     8401 ctgattcact gtatttttgt agataacgct tccggtaaac ggaagagggt gcagagatta
     8461 taaagcgctc tgcgcgcgcc ctcctaccct cctaatgtgc acagcaggtg cactgccctc
     8521 cttatttgct tacgcaaaaa gttactgaga gaaaccgttt accatagccc tgagtgagtc
     8581 agcctgtcga atcctcaaac agcgggtcgc catagtgata ccccgaagct agcaaatcgc
     8641 agatatactt gtcaagagaa cgttaactat ttataaaaac aatctcccct cgcatttaag
     8701 acgaggggag attgttggtg ggcgcgcatg gattcgaacc atggaccggc aaggtataag
     8761 cttgctgctc taaccactga gctacgcgcc cgcaaagggg atatgcaaaa tatgtgctta
     8821 ttctaccagg aacaggggag tgactcaagg aaataacacc aactcccctg ctctcttcgt
     8881 ttttatctca aaagggggga ggggagtcaa aaaaatccct ctcccctttc tcttggtaca
     8941 cccactatta atcaagagca ccaagtgtct gaattaccgt tatctttctc cctctttttt
     9001 tctaaagagg ggatagggga gagaacctac tgtatgtgta cttctctcct acgacagaat
     9061 acgctcggcg gacttctcga ttacccgcca gtcaagattt gcaaaaaaaa catcaatata
     9121 atcccctcga gccgggccat agtctcccca atacgcatgt tcatagacat caagtgctac
     9181 caaaggcgtt gcgttccaga ttggatatgt attttgggta tcgccaatat agttaaagag
     9241 ttttccagag tcatgatcgt acgctaacca aacccaacca cgaccggcaa tgccagttgc
     9301 tttcatatct tgcttaaacg cctcaaacga tccaaaatct cgatcaatca tctcagctaa
     9361 ctttcctgtt ggcgcagaac catcgccacc caaatgacta aagtaaatct cgtgattttt
     9421 gataccacct acggcaaaag aaaattcgac ctttaactcg cgaatatcac tatacgtttg
     9481 gttcgcttta gccggatctc ggtcaacggt ttctaacttt tcaagaattt cgttcgcctt
     9541 tttaacatac ccttggtaga gcttatagtg cccttccatc gtttgctttg atattccttg
     9601 gatttctttt tccataacgg agaatgtctt ttcagtatac atgaatactc ctttcgctag
     9661 cttagctagc atattttatt ttgccgcgcg caagaacata ctatcaccct tgttcccaca
     9721 cccgttcgac tatattcttt tcttggttat agaatcgcgc caaagtaaag agccaatcgg
     9781 agaggcgatt aacccactgc aacacctctg gttcaacaat actcccctca tcttcaagtg
     9841 caactaagag ccgttcacat cgacgaataa ccgcccgagc aatgtgaact tgagctgaaa
     9901 gcacaccccc ccccggaaga ataaactgag ttaaaggagg aagatcttga gtcatgacat
     9961 caatagattt ctcaaactca ccaataaaat cagcggtggg aagcgttagc tttaagtgac
    10021 tttgatttga cggttccgca atccgagccc cacagctaaa taacattgac tgtgctttta
    10081 acagtaagtc taacgacgta tggtccgacg agagaaacgt aatcaacaaa cccaagtggg
    10141 cgtttgcctc atcgaacgtg ccaagaactt caatcctcaa actagattta cggattcgag
    10201 atccgtccac cagtcctgtc gttcccttgt cacctgtctt tgtgtagatc ttcattactt
    10261 ggcttgtacc aaatccaaag ccggttgacc agagagcaca gccaagatat tcgccactgc
    10321 tttttgagcc atagcctctc tggcttcaaa agtagccgat gccgtgtgcg gcgtcatgat
    10381 aacattctcc agcgacaaaa gatcaggagt tatatttggt tcgttctcat acacatcgat
    10441 agccgctccc gcaatcaact tacgcttaag agcatctgtc agcgcagcct catccacaac
    10501 agggccacgc gacgtattca ccagataagc agtcggcttc attgtcgaga gacgtgccgc
    10561 atttactaaa tgtttagtcg ctggcagcaa tggaacatga accgttacta catcaccaac
    10621 agaaaatact tcatcaacag aaaagtattt tgcattaaac tttgattcaa aatctggatt
    10681 cggtcttatg tcagtataag caatcgccat accaagccca aatcccgcaa tgtgcgccac
    10741 gcgcgaccca atatgaccca aaccaactat agaaagtact ttaccggata attctggtcc
    10801 attaaatatt ttcggatccc agccagagta ttttccttgt cgaacaaact tatcagccaa
    10861 cggaatatgc tttgcgagcg acagcataag cgtaatggta tgttctgcta ctgcattatc
    10921 ggaagtacct ggagtattcg taacaacgat accacgttgt gccgtcgccg ctaaatcgat
    10981 atggtcaaaa ccaaccgaca tcgttgccac aatttttaat tggctccccg ctgcgtcaca
    11041 cacttctcca gtaatcttat ttgttaagag gcatagaatc gcatgtgcac ctttcacaaa
    11101 tccgagcagt tcttcaggcg ataaagcctc ttctttctca ttcacagcga cttcgtattt
    11161 ttcccgcaac agtgcgagag ctttgtcgct aattggccag gttaccacta cttttggttt
    11221 tggttccata gagtctcctt acttaacaat attttgcttt taaatgccca ccgaagaggg
    11281 caattttctg ttttaccact tcgcgcatcg cctcggtccc gggagcaaga atactgcgag
    11341 ggtcatacaa ttttggatcc gatgccagca cctcgcgttc agcttccgaa aaagcaatcc
    11401 gaagatccgt atcgatgttt attttcgtaa tccccaaagc aattgcttgg cggatatcat
    11461 cctctggcgt tcctgaagcc ccgtgcaaca caagggggag ggagacagcg gcggcgatat
    11521 gctcaagctg atctaagtga agcttttcac ctggttttgg cttaccgtga ttattcccga
    11581 taccaaccgc cagcgcatca cagtgtgttg cacgaataaa ctcaaccgcc atacctggat
    11641 ccgttaaccc ctcttcggcg gagatatcat cttcgacccc cccgacacga ccaagttccg
    11701 cttccacgga aatccctttg cggtgagcac gatctaccat ttctttagtc catcgtacgt
    11761 tctcttcgta cggcaaatgt gacccatcaa acataattga tgaatatccc gcctcaatcg
    11821 catcgtacaa aacacggtgc gtacggccat ggtcaacatg aaatacaact ggcacaaatg
    11881 atgccttagc acgggtcagt acgatggccg ccaactccga catgcccgca tacgtaatcg
    11941 ccttttcact cgtctgcacc actaccggcg catgcatttc ttcagccgcc gaaataaccg
    12001 cttcgactga ttccaggttg tagacattga acgccccaac agcaaacttg tggtgctgcg
    12061 cagcacgaag aactgggttg agtgtgacaa gcattacttt gacctccgta gattccttaa
    12121 aaactcacgc cgacggacaa ttagtaataa agctaaccca ccgagtaaaa cgtaaacaat
    12181 gttaaggtaa cctataaaaa taccttggta aactaaacct gaaaaagcaa agtatcctgc
    12241 tgcaataata ctccgggaga ggacaaacaa tatcgttagg acaagtgcaa ggcggctcgg
    12301 acttaaatac gctcccacga aaaatacaat cataagactg aagaataaaa caggatggat
    12361 atttatgccc acccaaaaag caacctgagc accaagccaa gcagccgcta ctagccaaag
    12421 aagaaccatg ccgagtggat atttattttg cattacgatg actttctctt tatggcccgc
    12481 tttacagcat cagtaatgga cttacgtgtt aacccataag tcgcccacag ttcattaggc
    12541 tgaccagact gcccaaaaga gtttggcatt ccaacgcgtt caaccggaac cggatggtgc
    12601 tcggctaaga gttcacacac ggcactccca agcccgccat cgatttgatg ctcctccacg
    12661 gtcactaccg cctttgtccg tcgtacactt ttaagaatgg tatcttgatc aagaggttta
    12721 atagtcggac aaaagataac atcgcacgta atatccctat cactcagccc ttctaatgcc
    12781 tgtagtacct cataagagat cggcccggcc gtgataattg taacgtcgtc accagctaag
    12841 gcatggattg ctttcccaac cacgaaaggt tgggaagacg aagtaaagac cggagacttc
    12901 tcacgagtta aacgcatgta agccggcccc tcgtatttag ctaaagctat agtggcttga
    12961 tacgcctcgt tactatcagc tggagcaaca acaataaggt tagggagaac ccgggtaatc
    13021 gcaatatctt caagcgcttg atgggttgct ccatctggcc caaccgataa accagagtga
    13081 cagccaataa cctttacatt ggcatccgag tagcagacac tgacccgaag ttgatcccag
    13141 tttctcccag ggctaaatgt agcgtacgaa gttataaaag gaatttttcc tgataaagcc
    13201 aaaccggctg caattcccat catattttgc tcggctacgc cgacttcaaa gaatcgttca
    13261 gggaaagatt tctggaagcc gtccatccgg gtcgattcgc tcagatcgcc cgtcaatccc
    13321 acgactcttt catcttgttc gccaatcgca accaaggctt ttccaaatcc ttctctggtt
    13381 ggagcctgtt ctatcctttt ctcatcgagg taatctttca cgaaatagtt actcatgctc
    13441 gctccttatc ttaccgccaa gtgtacggag ctccttgaga gcaattgctg cttcttcggg
    13501 tttaggcggc ttaccgtgcc aatgatagtc gtactccata aagtcgacac cttttccagg
    13561 aatcgtatgc gctaagatga gagtcggttt ttcaacaatt gctttggctt gatcgcaggc
    13621 atcaataatt tggcgcatat tatgcccgtc aacttcaatc acatgccaac caaacgcttc
    13681 atatttttcc ttaatcggct caagcgccat aacactttcc gttggtccgt caatttgaat
    13741 attgttgcga tccataattg ctgttaggtt attaagctta taattaccgg cgaacatcac
    13801 tgcttcccag atttgacctt cttgctgttc tccatcagag aggacacagt aaacccgatg
    13861 cctttcttta tttaaacgcg cggccaaggc cataccaatt gcttgcgaga gcccctgacc
    13921 tagagggcca gaacttgtct ctaccccagg cgtttcgaga ttatggggat gaccatgcaa
    13981 gcgggttcca aatttacgaa gcgacgaaag ctcacgaatt ggaaaatatc cagcataggc
    14041 caagctagca tagtacacgg gtgcaatatg cccgcacgaa agtataaaac ggtcacgtcc
    14101 ttcccacttt ggattccgcg gccgatgctc cataacggca aaaaagaggg ccgtaaacac
    14161 atccgccata ccaagtggac cagctgagtg acccgaaccc gccgacacaa gcattttaat
    14221 aatggctttg cggatctcgt ttgctcgcat ttcaagctgc ttggtagtca taggctcctc
    14281 caataggatc acttaaatgt tcatacatac gaaacgcttg gaccggatgc gtttgatgaa
    14341 agatcgcgct tcccatcacc aagttatccg ccccgtgagc aattagctga cggatattct
    14401 ccttgccaac accgccatcc accgtgaccg aacaatcagg ataatgttca gatataaaga
    14461 ggagccgatc atacgttgca gagataaatt cttgtcccat ctgcccaggc tctatcccca
    14521 tcatgactac acccgagaga tctggtggaa gctggagact ggcgagcgga gtaaaggggt
    14581 ctatcgccaa ccaggtatcc actcccttgt tcacagcact aatataatct ttttcccagt
    14641 ttttactctc aacatggata atgagccggt caattggcaa atcagattca agaaccttaa
    14701 gcgggaagtc tgtcatgcag tgaacttcaa ccgccatctc accaaaatca aggctactta
    14761 agtcctcgag agagagagat tcaaatttat agagcgagtg atcaatataa tctacgtgaa
    14821 tccaaccact catttctcgg gccagtaaaa tgcgctcagt agcgattcgc ttattggttg
    14881 tgaggagggc tggaataatc atttaagtgc ctttgcttga tgaacctgac gcttaccgag
    14941 tcgaatttca cacgtctttt tttgtttgag ttcacgcata cattcgtcca tttctatgag
    15001 ctccatcact ttccgtacta aacgttcagg attatcatca attgctactt ttgatttccc
    15061 gcgctgactc acctccataa tatgctcaag ttcatcagca acgcctcctg taccagttaa
    15121 aataccgatt ggctttctat cttcaaatgc aatggtaaat tcatttagcg ttcctatgcg
    15181 cccgcatata aagataatcg cgtcgcatga acgtataaaa aggagatttc tacccgaata
    15241 ttgaaaccca gtatacataa taatatccat ctcatctttc ggcagacggt attttcgctc
    15301 atgttcggtt cggcttgtag cgggagagat accaaccgtt atacccccgg ccgctttagc
    15361 gccatgagcc gccgcgtgag ggatcccaac agtgtcgccc gtaacacaaa ccgcgcccgc
    15421 tagcgcaatc tggcggccga tttcacgcga tttagcatag gcgcccggag cacagttatt
    15481 gacggcagac cctgaaactc caatcttata ttggtatgcc atattccctc ctctcacact
    15541 caattcatcc tagcatatct aatttttcta cgcgacgaat atgccgctct tcctcaataa
    15601 agtctgtttg cagccacgta tgtacaagcg ctagattctc ttcgttactg agaagtgcct
    15661 cacccaaaca gaggacattt gaattgttat gaagccggga cctctctgcc ataaattcag
    15721 tcgtgactaa agcggcccga atgccttcat acctattggc agcaatactc ataccaatac
    15781 ccgtatcgca aataagtatc cctcgcgcac tatgctctaa aacctgctca cacactttct
    15841 gagcatagtc tggataatcg tcctgtttat ctaatctctc tggcccgcaa tcaatcacat
    15901 catactttga tgttagttcc tgtattaaaa aacttttcag agaataaccc gcatgatccg
    15961 agccaataaa aatagtatac ataaaaatat tatctggggt tatgcttcga ttgtcaaaaa
    16021 aataagaggg gtggcccaga ttactctgga ccaccccgga aaaccaagat ccgcttactt
    16081 ggcagttcgg tacggctcaa gtgcgcgatc aacggcttcg attccatgca actgcacgag
    16141 cccagcgagc gtcggatcga gatcaatctg ggctttctca aacctgcccg cgtgcctcag
    16201 ccagtcacga acacggagga tacgctgatc ccatcccggt ggcgccgcgt acacatccaa
    16261 gtacgtatca tcgatgtcac agacagcgag cgtatagaac gtactgtaac gaccggaacg
    16321 tggcgcttca ggttcgccgg cataccacat ctctcggtga accacctgga cgatcctact
    16381 ctgcaatggg actacggcga ggtggctatc cgacgtatcg ggcatatacc acacgaacgt
    16441 agtccgcgcc atgataggct tctcctgcag tcgcttctga aagcggtcga gatcgtaata
    16501 gaacctaacg ttcgccggct ctacccggaa gggagggagc ttctcttgct tggttgcaat
    16561 gtctacaagc aggtagtaga gcgcattatc ttcttcggga gattcgtgaa cgcgccactg
    16621 agagcagcca ggaacggccc cttctaaaca cgccgtagca gcttcatgag ctccacggct
    16681 caagcaccaa tctccaggac taagctcgcc tacgatcttc caaaagtcat gccccgtagt
    16741 cattgttgtc tcctttcgag ctcaccacca ggatgagtga tcgatgcggc actattgcca
    16801 ttttcgaccg aacaacaagt gtaatatact attatattct ttttatttca aggctaaaac
    16861 ccagtccgtt tgaataattt ttctaaatca gtacccgaga tttcaatgtg gcttggtcga
    16921 ccatgcggac aggtaaaaga ttctgcttcg agcaagtctt ttatgaggcg ctgtttttca
    16981 ccctcgctaa taacatcgcc ggccttgact gcggccttac aggccattgt cgcaaacagt
    17041 ttatgcttaa agtctgtcag ttccggctcc acacgatctt ccgtaagccc acctaatact
    17101 gaccgaaaaa atttaaccgg atcactcagc ttcattacct gaggaacttc gagcaaataa
    17161 acatcgtctc ctgcggtaaa agcaaagccg atggcctcaa aatcgctctt atgaacatga
    17221 aacagagccc gttcgtcggg gctgagtgat agctcggctg gcaaaagtaa tgcctgacgc
    17281 accacttctt tctgcttctc ctgtgcccag agctgattat acaaaatccg ctcgtgagca
    17341 gcgtgctgat ctactagcaa gagctttctt tcatgctggg caacgagaaa acagttatca
    17401 attgttatga tcggggaaga ctgaatctcg gtcggctcat taacaaactg ccgcatggct
    17461 tgaaaaggcg aggagctaaa agaagagcgt tgtgtgcctc ctccaaaact cgtccctgat
    17521 tctaaaaacc ctccccgctc aattcgttca aacggcatac tctcgagctg agtagaagga
    17581 gaagcatacg gagccgatgg caccgaaaag gttggcagcg aggtaaacgc attattaacg
    17641 cccgcaagag actgagaaac ggactgcatg acaaaacgaa aaacctcctg ggagttcaca
    17701 aaccggactt cgtctttccg tggatggata ttcacatcta ccataccagg atgaataaca
    17761 aggttaagag cgtacgaagg atacatcgaa ggaggaagaa gtgttccaaa cgcatcctta
    17821 atcgctcgtg caaccatttt atcgttcacg ggatggccat tcacggcgaa aaattgtctc
    17881 gctttgccat catgagcaat cataggctgc gctaccacgc ccgaaagtgc catatgcggc
    17941 acttctcgat ccacggaaat caaagcttgc ggctctacct tccagacttg agcaacacgg
    18001 gaagaaaaat catgcgaggg aagatcgtaa atcgtttttc cgttatgttt taccataaac
    18061 gcaacttcgg gatgcacaat catttgcttt acaagtgttt ctaacgtggc cttccactca
    18121 gtcgcttcac tttttaaaaa tttttgtcgc gctggtaaag ctgaaaatag atcacgaact
    18181 tgaatccgag taccttttac taacgggtga ggcttcgggg aagacagaac accgtgctcc
    18241 atcaccactt gcgtcgcctc agagctatct gcttttcctg ttactatctc taccactgcg
    18301 gacgcaccaa tagccgctaa cgcctcacca cggaatccga gcgtcgtaac gtgaagcaaa
    18361 tcatcgactt cttttatctt cgaagtacca tggcgcaaga agcaggaaac agcatcttct
    18421 cgatccatac ccgagccatt atctttcatc tcgataaaag tcttgccgcc atcgtccagg
    18481 ctgatttca
//
LOCUS       DASWYB010000028        14638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_833025, whole genome shotgun sequence.
ACCESSION   DASWYB010000028 DASWYB010000000
VERSION     DASWYB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14638
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833025"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..1080)
                     /locus_tag="VGE59_01520"
     CDS             complement(415..1080)
                     /locus_tag="VGE59_01520"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY1074361.1"
                     /translation="MIPLRDSKPSHHFPLWTIILIILNAAVFYWELTSPESDAFIHTY
                     GLVPALIDNRNPATWYPFVTSLFLHGGFLHLGLNMLFLWVFGDNVEDHLSLFYVPFYI
                     LGGILGALAQYVFMQGADIPIVGASGAIAAVMGAYIVYHPHSRIQTLVFILVFITIIE
                     IPAIVMLGYWFALQLLSGYSSINDIAAGGGGVAYFSHIAGFVTGLVVGWATKPYQLRP
                     SRV"
     gene            1275..2126
                     /locus_tag="VGE59_01525"
     CDS             1275..2126
                     /locus_tag="VGE59_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1074362.1"
                     /translation="MDTYRFPSGGVPRQQQDWPGHESSLTPLADHGETSYVGSGKLVG
                     KKALITGGDSGIGRAVALAFAREGADVAIVYFNELNDAKRTVEIITEAGQKGYMYEGD
                     VSEYSVCRNLVKQAVNDLGGLDILVNNAAYQQEQPSFLNITPEQLERTFKTNIFGYIW
                     MAQAVLPSFASGGVIINTGSVTAMEGSSSLIDYASTKGAIHAFTKSLAKAVSSQGIRV
                     NCVAPGPVWTPLIPSTLLPDHVEKFGSDTLWKRPAMPAELAPTYVFLASEESSYYTGK
                     VFDPTGN"
     gene            2151..2375
                     /locus_tag="VGE59_01530"
     CDS             2151..2375
                     /locus_tag="VGE59_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEY1074363.1"
                     /translation="MPRGVGGKGPANIMRHLKGVHFPATRQDLIEHVESKDGQEGYPD
                     TAKVLEVILSLPDEQFSSMSEVMHHTRSPK"
     gene            2414..2887
                     /locus_tag="VGE59_01535"
     CDS             2414..2887
                     /locus_tag="VGE59_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074364.1"
                     /translation="MNEFTAKKLGEVLAFCRVGAETIEKGHEGFVKVADVEKLEKTIS
                     QLNNHADMIIDFADKAGMKEVTLTKCEGTSNKLRAMRDMYVGEEWDNAAELLEWKGFF
                     EGAAIVHWALVEGAAKSLDLAELADLADDAQDKHEDLLEFVSKSLEKIGSEKAKA"
     gene            complement(2921..3841)
                     /locus_tag="VGE59_01540"
     CDS             complement(2921..3841)
                     /locus_tag="VGE59_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY1074365.1"
                     /translation="MQKPVSSRRVVLTSFVVDLSDVLLNTFIAVVSGSVVMASEALQG
                     LADLLTSGLLLIGLKQSRRRANKSHRFGYGREIYFWTLMSGIMMLTLTAGLSFYFGLN
                     RFFHPEPIDKTPLALGVLLIGLATNAYACYLSYQRLHAFHPRQKLWKTFQYSDLIETK
                     ATFILDSMGALAALFGFLSLGLFAITGNSRFDSVGAMLIGVLIAVLSFFLILEVKDLL
                     VGRAASPELEEKIRESALSIKGVQHILDLRTMYLGTERLLVNLEIHVKDNLTTDQIEQ
                     LTDAVKHRIKQRIPSVKHIQVELESPDEEL"
     gene            complement(3888..4232)
                     /locus_tag="VGE59_01545"
     CDS             complement(3888..4232)
                     /locus_tag="VGE59_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEY1074366.1"
                     /translation="MNQEDIFDLNLARIKRFEKIEGRPWGVEGAIIELTKQVGELAAL
                     VMTKEGYYFKNREQYSDKYSQNTDDIADELADITYAVTRIAKHYNIDVLKSTQKAAQE
                     DERWFKDKGISF"
     gene            complement(4262..4337)
                     /locus_tag="VGE59_01550"
     tRNA            complement(4262..4337)
                     /locus_tag="VGE59_01550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4302..4304),aa:Arg,seq:ccg)
     gene            complement(4347..4769)
                     /locus_tag="VGE59_01555"
     CDS             complement(4347..4769)
                     /locus_tag="VGE59_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074367.1"
                     /translation="MSLKKPFRAVAIFVDQDRILLMERIKSDNHYFVFPGGRIEVGET
                     PEEAVKREAHEETSLRILQAELIFELNNGSDHEYYFLVKEFTGELALGGEERDNMTAN
                     NQYILRWVPLNNMASLSNLFPLEGKEQLIELLSKTNKL"
     gene            5017..5262
                     /locus_tag="VGE59_01560"
     CDS             5017..5262
                     /locus_tag="VGE59_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074368.1"
                     /translation="MHEDHTGIPSYLMGHRPSPPSALSSTELCRRRSAGIPAHVAFMM
                     DAEAGARASAALASRHPKALRASGFTPDACLRAHTYG"
     gene            complement(5418..5996)
                     /locus_tag="VGE59_01565"
     CDS             complement(5418..5996)
                     /locus_tag="VGE59_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074369.1"
                     /translation="MVTHMTIESIDQIPDFPTSMHVPLLKLMLINNRGYICLAGWTRL
                     PVGSEVTLISEVTITGNEARGWFKPTTVKTSGATLSMIELVSPARSKERPEECTLILS
                     EVYEQIIVKALGEEGTVSCSTILTEEPLTCWEVYTAGHWVSMIALPREEVTSSLVSYF
                     SQLQRKGMAKLQESLLGQAKSRTLSALGLPKK"
     gene            complement(6143..7270)
                     /gene="ftsZ"
                     /locus_tag="VGE59_01570"
     CDS             complement(6143..7270)
                     /gene="ftsZ"
                     /locus_tag="VGE59_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY1074370.1"
                     /translation="MEKAPQIETFATIKVVGVGGSGCNAIRRMIAAKVRGVEFIAINT
                     DAQALLHNEAPVKVQIGKELTRGLGAGGDLEVGAKGAEEDKNEIYEVLKGADMIFVTY
                     GAGGGTGTGAGPVVASIAKELGALTVGVVTKPFSFEGLRRKKIAELGIEELKDKVDTL
                     ITIPNDRLLQVIDKKTSLSDAFAIVDDVLRQGVQGISDLITLHGIVNVDFADVRSIMK
                     DAGSALMGIGRAGGDNRAIEAARAAIDNPLLDVSIEGAKGILFNITGGPDMSMYEIDE
                     AAKAITEAAHPDANTIFGALVDEAMSGEIKITVIATGFDAEDLKEVRRPQIPMSSSAP
                     KMPLRNREPEPDIDLTLEAIDEDDSNDHELDVPAFIRRKLK"
     gene            complement(7365..8621)
                     /gene="ftsA"
                     /locus_tag="VGE59_01575"
     CDS             complement(7365..8621)
                     /gene="ftsA"
                     /locus_tag="VGE59_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY1074371.1"
                     /translation="MARQHHAGHISVGLDIGTSKIAICIGTLQEGIPTILGMSKSPSA
                     GIRKGVVSDIEETISALSAALAAAERIAGLPIHSATVGIEGAHITTTNSKGVVAVNRS
                     NSEINPEDTHRAIEAARAVALPPNQEILHILPRLFSVDGLQGVKDPSGMVGIRLEVDT
                     YIIGASTAAIRNMSRVITSAGLQVDNLLFGPLASATALIDKPAKESGVILVDFGASTT
                     SLIVFEEGDVIHATVLPVGSSHITNDIAIGLRTNLETAERIKQDHGYALSSEIGDKEI
                     IDLSEYNPAEESTTSRKYVCEIIEARLGEIFSMIRDELRIIGKDGMLPAGVVFTGGGA
                     HLAGLTDAAKDILQLPAQIGTPSSEISGMIDKLDDPLYSTSVGLMLSGLYQPHQELRS
                     PAYGKMDHQMGDIFEKAKGFLKHLLP"
     gene            complement(8648..9097)
                     /gene="ybeY"
                     /locus_tag="VGE59_01580"
     CDS             complement(8648..9097)
                     /gene="ybeY"
                     /locus_tag="VGE59_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY1074372.1"
                     /translation="MINFVLQNSTNHHIDDTTIQKGLAAAATKLGEIGERHVAIEIVT
                     SEESQALNAELRGQDKPTDIISISTQETKVGEQVIEESAEGALSFEINESKPLENAWP
                     VIGQLVLCYDVIAQNAQRAGQPVERELEWVIEHGILHLMGFHHEHDD"
     gene            9282..9485
                     /locus_tag="VGE59_01585"
     CDS             9282..9485
                     /locus_tag="VGE59_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEY1074373.1"
                     /translation="MKMSTLDWVAYILVVIGGINWGLVGAFDYNLVDSIFGDGSSIAR
                     IVYVLVGLSALYLLATLGRMQKS"
     gene            complement(9547..10041)
                     /locus_tag="VGE59_01590"
     CDS             complement(9547..10041)
                     /locus_tag="VGE59_01590"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEY1074374.1"
                     /translation="MHKETYIGWLNDAYSMEVGLVPVLENHEKDAEGNTEVQEKIQEH
                     IEQTKKHAEMIKACIERNGEDVSSGKETFGSMLGKMKAASTGMYKDEVVKNALADYTA
                     EHFEIASYQSLIAAATEMEDDETKQVCQTILGEEEDMAAWLAEQIPTITKDFMRQQAE
                     KEDE"
     gene            complement(10205..11353)
                     /locus_tag="VGE59_01595"
     CDS             complement(10205..11353)
                     /locus_tag="VGE59_01595"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY1074375.1"
                     /translation="MKKMLGVIGGGQLGFMLTLAANRLGIPVVVLDPNQDCPATHAGA
                     MQIVGKFTDTNRLRQLADRGTAGVTVEIEHVGVETLQQLVATGYEINPAPSALALIRD
                     KYTQHRMWLEHDLPTPRIMTVDGSLGGAVFAARKAWETQKLVVKTRFGGYDGRGTAFI
                     STDEDIKTAVRKVGDQHLYLEEAIPVAREIAVIMYRDYTGTVGHYPVIETFQQEGICH
                     HAMMLGHQDANLDRRATNLGRKVMEHLEGRGVFTIEMFVTGEGDIFINEVAPRVHNAG
                     HLTVEGCETSQFEQHVRCVVGLPAGRPNSRHLHNGVAMVNVLGNRNANANPQGLEHAL
                     QIPGVSFTDYWKDEVREGRKMGHFVCVDPRGPQYALKGAQEARRRFTI"
     gene            complement(11441..12136)
                     /locus_tag="VGE59_01600"
     CDS             complement(11441..12136)
                     /locus_tag="VGE59_01600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY1074376.1"
                     /translation="MHSITPARVLAAANYLLVSLTIASLIGMVSPTLLAAYIEKNLIP
                     QPAQSFEAPVTASVSGVSTTTREDSADFFQYREGTAVPLTTSSSSNVARNTARNTIIA
                     KAQAADTHISASGYMLPIKYRGISRGIITGVHTGIDFLAEVGTPVWAAADGTVTTADA
                     SGWNGGWGKTIVITHSESQNTRYAHLSKLIVSEGQVVKRGDVIGYSGNTGRSTGPHLH
                     FELRINGKPRNPF"
     gene            complement(12296..12748)
                     /locus_tag="VGE59_01605"
     CDS             complement(12296..12748)
                     /locus_tag="VGE59_01605"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY1074377.1"
                     /translation="MTLAEQIQKDTIAAMKERDADRVAVLRMVSSAIKNEAIKVGGLG
                     TVLTDPQTLAVLNREVKQRKDAATQYKEAGRPELAEKEEAELAIIEEYMPKGLSDEAL
                     QSLVNEIITETGATTRADMGKVMAAIKAKLENPADSAKAAQLVGQKLQ"
     gene            complement(12825..13058)
                     /gene="rpsU"
                     /locus_tag="VGE59_01610"
     CDS             complement(12825..13058)
                     /gene="rpsU"
                     /locus_tag="VGE59_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY1074378.1"
                     /translation="MSDDFRPRSRENSDRSDRPRGRENFEVVLRRFFREVQQSGILTE
                     AKRKRFFEKDTSRKLKRTIAQRKADRKRITRGY"
     gene            complement(13488..13844)
                     /locus_tag="VGE59_01615"
     CDS             complement(13488..13844)
                     /locus_tag="VGE59_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY1074379.1"
                     /translation="MPHESPCLFCRISNKDLSASIVYEDENVIAFNDIHPKAKVHVLV
                     IPKKHIQSLADAEDEDQELLGMVMLTVKKVAEEQRIASSGYRTIINTRTHGGQEVDHL
                     HVHILGGEAIGPMVSK"
     gene            complement(13750..14346)
                     /locus_tag="VGE59_01620"
     CDS             complement(13750..14346)
                     /locus_tag="VGE59_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074380.1"
                     /translation="MTHHSTDSPNENPTAKTKRSHTWLQQQLDIIWSQHFSDIERLNI
                     VTIEWGRTNRNRLGSITGKGGSRDNPTRSEIRINSLMRDERVPESVIWQTIAHELSHY
                     CHGFCSPHPQKYPHAHRGNIIEKELRSRNLAIVYEESEAWLKANWRNYLAQTIGTTRR
                     KAPIRRTYAPRITVSLLQNFKQGLIGQHRLRRRKRDSV"
     gene            complement(14288..>14638)
                     /locus_tag="VGE59_01625"
     CDS             complement(14288..>14638)
                     /locus_tag="VGE59_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY1074381.1"
                     /translation="QAVHLIGMPEAQLTLAQGVTYLANAPKSTAPMDALYAAYYDIDN
                     LPIGPVPMHLRNAANQVMKDLGAGKGYVRYEKNDPEQFKNQQFLPDNVKHHTYYDASL
                     DRQPKRKSNGENQA"
BASE COUNT         3417 a         3413 c         3680 g         4128 t
ORIGIN      
        1 gggtgcactt cttctttggt tagtttatta gaaagtgtga ggtgaggatg aaaggtttca
       61 tgttgcagta cggtactttc tgggtagaga gaaagagccg attgcacatt gctctgcagt
      121 ctggaaagtt ccgtgctcgg cttgacgtca gcataaataa cagagtgttt gtgttgaaag
      181 acatttatgc cggtgagttc tatgggaaag ggactctgtg gtggaatttt ctgcttcagg
      241 taatcaccta tcagcctact ctccgatttt gattcaaagc gtggaacaag ggtgatatgg
      301 ggcccaataa tacggttcta ccgtatagat cgatatgttt cctgaatttc tgatagtgcg
      361 ttgcttaaat cgtcgggcgg ttcaatggtg agtaaatagg tagccacctt tggattatac
      421 tctggacggc cgtaattgat aaggctttgt ggcccagcca acaacgaggc cagttacgaa
      481 tccggcaata tgagaaaagt atgccactcc cccacctccg gcggcgatat cgtttataga
      541 tgagtagcca ctgagaagtt gaagtgcaaa ccagtaacct aacattacaa ttgctggaat
      601 ttcgatgatt gtgatgaaca caaggataaa gacgagtgtc tgaatccggc tatggggatg
      661 atatacaatg tatgccccca ttactgctgc gattgcaccc gaggcgccaa cgataggaat
      721 atctgcgcct tgcataaaca cgtattgcgc cagggcacca agaatgcctc ccaggatata
      781 gaatggtacg tagaataaac taaggtggtc ttcgacgttg tcaccgaata cccataaaaa
      841 cagcatgttt aagccaaggt ggagaaatcc tccgtgtaaa aataacgagg taacaaaggg
      901 ataccaggtt gcgggattcc tattatcaat tagggctgga acaaggccgt aagtatgaat
      961 gaaagcgtcg ctttcggggc ttgtgagttc ccaatagaag accgctgcat tcagtataat
     1021 taaaataata gtccacaacg gaaagtggtg gcttggttta ctatctcgta atggaatcat
     1081 agttgcagta tatcaacctc acattaagcc tgctttgggt aatatcccta aatgtgacta
     1141 cttagcctat gtgtattgtc tgctctacga aacttgactg tttagagaaa agtctaagga
     1201 gaaaacgtaa gacgctttgg gcttttctac tttggctaaa gccctcttca tcagaatttg
     1261 gtatacagta cagtatggat acttatcgct ttccttctgg tggtgtgcca cgccagcagc
     1321 aagattggcc tgggcatgaa tcgtctctta ctcccctcgc cgatcacggc gagactagtt
     1381 atgttggctc aggaaaatta gttggtaaaa aagcccttat tactggagga gacagcggaa
     1441 ttggccgggc cgttgcactt gcgtttgccc gtgaaggcgc cgatgttgcg attgtttact
     1501 ttaacgagct caacgatgcg aagcgcaccg tcgaaataat cactgaagcg ggccagaagg
     1561 gctatatgta tgaaggcgat gtatctgagt actcagtatg tcgtaatctt gtaaagcagg
     1621 ctgttaatga tttaggcggc cttgatattt tggtgaacaa cgcagcgtat caacaggagc
     1681 aaccttcatt tctcaatatt acccctgaac agctcgagcg gacatttaaa acgaatattt
     1741 ttggctatat atggatggcg caagccgttc ttccttcttt tgcatcgggc ggggtaatta
     1801 ttaatacagg ctcagtaacg gccatggagg gaagtagttc gctgattgac tatgcctcga
     1861 ccaaaggagc tatccatgct tttacaaaat cactcgccaa agctgtctcg agccaaggta
     1921 tacgagtgaa ctgtgtggct ccaggtcctg tgtggacacc tcttattccg tctactctct
     1981 tgcctgatca cgtagagaag tttggaagtg atacgctttg gaagcgtccc gctatgccgg
     2041 ccgagctagc gccaacgtac gtttttttgg cgtcggaaga atcaagctat tacacaggaa
     2101 aagttttcga tccaacaggc aattagtctg ttatcgagaa aggcgtgtct atgccacgag
     2161 gggttggtgg aaaagggccg gctaatataa tgagacatct taaaggggta cactttccgg
     2221 cgactcgcca ggacttaatt gaacatgtgg aatcaaaaga tggccaagaa ggctatcccg
     2281 atacggcgaa agttcttgag gtcattcttt cattgcccga tgaacagttc tcttcgatgt
     2341 cagaagttat gcaccatacc cgcagcccaa agtagcattt ctggtataat agacatataa
     2401 cgaaaggatt ctgatgaatg aatttaccgc taaaaaatta ggcgaagtgc tcgccttttg
     2461 ccgcgtggga gccgaaacaa ttgaaaaagg gcatgagggt tttgttaagg ttgctgatgt
     2521 tgaaaaactc gaaaagacca tttctcagct caataaccat gccgatatga ttattgactt
     2581 tgccgataaa gctggcatga aagaagttac tctcaccaaa tgcgaaggaa ctagcaacaa
     2641 gctccgcgcc atgcgtgata tgtacgttgg cgaagaatgg gataatgcgg cggagctttt
     2701 agaatggaaa ggcttttttg aaggagcggc aattgtgcac tgggcacttg ttgaaggagc
     2761 tgcgaagtca cttgatttag ctgagctggc tgatttagct gatgacgctc aagacaaaca
     2821 tgaagacctt ttggaatttg taagcaagtc gctcgaaaag attgggagcg aaaaagccaa
     2881 ggcgtaagca aaaatacccg cttctcggaa gcgggtattt ttaaagttct tcgtccgggc
     2941 tctcaagttc tacttgaata tgtttcacgc tcgggattcg ctgtttgatg cggtgcttaa
     3001 cagcgtccgt cagttgttca atttggtcgg tggtgaggtt atctttaacg tggatttcga
     3061 ggttcacaag cagtcgctcg gtcccgagat acatggtccg gagatcaagg atgtgttgca
     3121 cccctttaat acttaaagca ctctcacgga ttttttcttc tagttccggt gacgcagctc
     3181 ggccgactaa aagatctttc acttcgagga ttaagaaaaa cgagagaacc gcaatcagta
     3241 cgccgatcag catagccccc acactatcga accgagaatt ccccgttatg gcaaatagcc
     3301 ccagtgagag aaatccaaac aaagctgcta aagctcccat actatcgaga ataaaggtgg
     3361 ctttggtctc tataaggtca gaatattgaa aagttttcca aagcttttgt cttggatgga
     3421 aggcgtgaag gcgttggtag cttagatagc aagcatatgc gtttgtggct aagccaatga
     3481 gtaagacgcc tagtgcgagc ggtgttttgt ctattggttc gggatgaaag aaacggttaa
     3541 ggccaaagta aaacgacaaa ccggcagtca gcgtcagcat cataatcccc gacatcaatg
     3601 tccaaaagta aatttctcgg ccgtagccaa accggtgact tttgtttgca cgacgtcgtg
     3661 attgctttaa accaataagt agcaacccag aagtaaggag atctgccagc ccttgaagcg
     3721 cctctgatgc catcaccacg ctgccgctca caacggcgat gaatgtattt aatagtacat
     3781 ccgaaaggtc gacgacaaac gaagtcagga ctacccgtcg cgaagaaact ggtttttgca
     3841 tatcctcact ctaacacacc caaagtgttt ttatgatggt ttctgtacta gaagctaatg
     3901 cctttatcct taaaccatcg ttcatcttct tgagcggctt tttgggtcga ctttaacacg
     3961 tcgatattgt agtgctttgc aattctcgtc acagcgtagg ttatgtcagc aagttcgtcg
     4021 gcaatatcgt cggtgttttg agaatattta tcgctatact gctcgcgatt tttaaaatag
     4081 tagccttctt ttgtcatgac taacgcagcg agttccccta cctgtttagt gagctctata
     4141 atggcacctt cgacacccca cggccgtcct tcaatttttt caaatcgttt tatgcgagct
     4201 agattcagat caaaaatgtc ttcttgattc ataatcaagt ctcctgtgat ttagttcgtt
     4261 atggcgcgcc cggagggatt cggacccccg accttctgga ccggaaccag acgctctatc
     4321 ccctgagcta cgggcgcatg tttgtattat agcttattcg tcttgcttaa taattctatt
     4381 aattgctctt tcccttcaag tggaaagaga tttgatagtg aggccatatt atttagagga
     4441 acccaacgca gaatgtattg attgttggca gtcatgttgt ctctctcttc tccaccgagg
     4501 gccagttctc cagtaaactc cttaactaaa aagtagtatt catgatcgct accgttattt
     4561 aactcgaata ttagttctgc ttgtaatatc ctgagtgatg tttcttcatg cgcttcccgc
     4621 ttcacggctt cttcgggcgt ttcccctact tcaatccggc ctccaggaaa tacgaaataa
     4681 tggttatcgc tcttaatccg ctccattagt aaaatgcgat cttggtctac aaatattgca
     4741 acggcacgaa aaggtttttt gagcgacata ctgttattat acctgctaag tcaattgatt
     4801 attatttgga atgttttcaa aatcccttga cgcgactttt gaaagccttt agaatggtgg
     4861 tgttcgcttg gcatataccc agtggattga ccttctagac gggctgaatc tgtccggagg
     4921 tcaatgcact cgctcaatgg ggagtgttgt gtatggccca acggacagct tgtccgtttt
     4981 ttcatttctt cggagatgga gatgcagaga gaggatatgc acgaagatca cactggtatc
     5041 ccgtcctacc tgatggggca tcgcccttcc ccgccttctg ctctgtcttc gacggagttg
     5101 tgtcgtcgcc gttcggcagg tattcctgct cacgttgcgt tcatgatgga cgctgaagcg
     5161 ggtgctcgcg cgagcgccgc actcgcgtca cgtcatccga aggcacttcg tgcctctggg
     5221 ttcactcccg atgcatgcct aagggctcac acctacgggt agtgtccatc gctcttcatt
     5281 cccggggttt tgccccttgt tccccgcctc cgccgtttac ggcgggggct ccttttatat
     5341 tttccttaaa aacaaataac tggcggccga gaaaagtccc aggccaccag ttaaatggag
     5401 agagcgtcgc gtgtcgacta ctttttggga aggccgagag cagagagggt tctgcttttg
     5461 gcttgaccga ggagagattc ctgcaacttt gccattcctt tgcgctggag ctggctaaag
     5521 taagagacta gcgagctagt tacctcctca cgtggaagag caatcatact gacccagtgg
     5581 ccagcggtat aaacttccca gcaggtcaaa ggctcctcgg tgaggatggt agagcaggaa
     5641 acggtaccct cctctccgag tgccttgacg atgatctgtt cgtacacctc ggacagaatc
     5701 agagtgcatt cttccggtcg ttctttagag cgtgctgggg agacgagctc aatcatcgat
     5761 aacgtggcgc ctgaggtttt taccgttgtc ggcttaaacc atcctcgcgc ctcgtttccg
     5821 gtgatcgtca cttcactgat cagagttacc tccgatccta ctggaaggcg agtccagcca
     5881 gcgagacaga tgtaccctcg gttatttatc aacatcaact tgaggagagg tacatgcatc
     5941 gaagttggaa aatctggaat ctgatcgatt gactcgatcg tcatgtgggt aaccatttcg
     6001 agctagcctc cttgaaggca ggtaagtccg ggtgattccg gacagcgtcg caatgacgtg
     6061 ataattatat actaaatgtt ttaatttgta aatatactgt gtatcgtaaa agcgcatcga
     6121 gtgatgcgct tttacttagt ttttacttta attttcggcg gataaatgcg gggacgtcga
     6181 gttcgtgatc gttagaatcg tcttcgtcga tggcttcgag ggtgagatcg atatctggct
     6241 caggctctcg gttacgaagt ggcattttag gtgcgctcga tgacattgga atttgtggtc
     6301 ggcggacttc tttgaggtct tcggcatcaa acccggttgc aatgacagta atcttgattt
     6361 cgccgctcat agcttcgtca actaaggcac caaatatggt attggcatcg ggatgagcgg
     6421 cttcggtaat ggctttggct gcttcgtcga tttcgtacat cgacatgtcg ggaccaccgg
     6481 taatattaaa gagtattcct ttagctccct cgatcgaaac atcgaggagc gggttatcga
     6541 tcgcagcgcg cgcagcctcg atagcgcggt tgtcgcctcc ggcgcggccg attcccataa
     6601 gggcggatcc tgcatctttc atgatcgagc gtacgtcggc aaagtcgacg ttaacaatac
     6661 cgtggagggt aataaggtcg gaaatacctt gcacgccttg acggaggaca tcatcaacta
     6721 tggcaaaggc atcgctcagg gaggtttttt tatcaataac ttgtaatagc cggtcgtttg
     6781 gaatcgtaat cagtgtatcg actttgtctt tcagttcttc aatgccgagt tcggcaattt
     6841 tcttgcggcg aagaccttcg aacgaaaatg gcttggtgac aacgcccact gtaagcgcgc
     6901 caagctcttt ggcaatcgaa gcaaccaccg ggccagctcc ggttccggtt cctccaccag
     6961 ctccataggt aacaaaaatc atgtctgcgc ctttgagcac ttcatagatt tcgtttttat
     7021 cttcttcggc ccctttggca ccgacttcta aatccccacc agcccccagg ccacgggtga
     7081 gttctttgcc aatctgaacc ttaacaggag cttcgttatg taaaagcgct tgtgcatctg
     7141 tattaatagc aataaattcg acaccccgaa ctttggcggc gatcatccga cgaatggcgt
     7201 tgcagccgga acctccgaca ccaacaactt tgattgtggc aaatgtttca atctgggggg
     7261 ctttttccat gagggggctc cttatacgtc cttagtatca ttgaattaag aataattcaa
     7321 gaatacgacc agtccctcct aggtcgatat ttatgtaagt gtagttatgg cagaagatgt
     7381 ttgagaaacc ctttagcttt ctcaaatatg tctcccatct ggtgatccat ttttccgtat
     7441 gccgggctac gtaactcttg gtggggctga taaagcccag agagcatcag gccgacactc
     7501 gtagaataga gtgggtcatc aagtttgtca atcatgcccg aaatttcgga tgacggggtg
     7561 ccgatttggg ctgggagttg caaaatatct tttgctgcgt ccgtcaggcc agctaagtgc
     7621 gccccgccgc ccgtaaagac gacgcctgcg ggcaacatac catccttccc aataatgcgc
     7681 aactcgtccc ggatcatgga gaaaatttcg cctaagcggg cttcgataat ttcgcagaca
     7741 tatttgcggc tcgtggttga ttcttcggct gggttgtatt ctgaaaggtc tataatctct
     7801 ttatcgccga tctcactcga taacgcgtag ccgtgatctt gtttaatccg ttcggcggtc
     7861 tctaggttcg tgcgtaggcc aatggctata tcgttggtaa tatggctcga tccaaccggt
     7921 aagaccgttg cgtgaataac atcgccttcc tcaaagacga taagcgaggt tgtcgaggcg
     7981 ccaaagtcga ctaaaataac accgctttcc tttgcgggtt tatcgatgag ggcagtggca
     8041 gaggctaagg ggccaaaaag caaattgtcg acttggagtc ctgccgaggt aataacccgg
     8101 ctcatattac ggatagcggc agtagatgcg ccaataatat aggtgtcgac ttcgaggcgg
     8161 ataccaacca taccgctcgg atctttgact ccttgtaagc catcaacgct aaagagccgg
     8221 ggtaaaatgt gtaaaatttc ttggtttggt ggtaatgcga cggctcgcgc ggcttcgatt
     8281 gcccgatgtg tgtcttctgg gtttatctca gagttgctac ggttaacagc cactactcct
     8341 ttcgagtttg tggtcgtaat atgggcgcct tcaataccaa cggtggccga atggatcggc
     8401 agcccagcaa ttcgctcagc ggccgctaac gcagccgaca atgcgctaat cgtttcttcg
     8461 atgtcggaaa ccacgccttt acgaataccg gcggatggcg atttgctcat ccctaaaata
     8521 gtaggaattc cttcctggag tgtgccaata caaatggcaa tctttgaagt gccaatgtct
     8581 aatccaactg atatatgtcc tgcatgatgc tgtcgagcca tgctaaacgt tccctccgct
     8641 accagaatta atcgtcatgt tcgtgatgaa aacccatgag atgaagtatc ccatgctcta
     8701 ttacccattc tagttcacgt tctacaggct gtccagcacg ctgggcgttt tgggcaataa
     8761 cgtcgtaaca tagcacgagt tgcccaatga ccggccaagc gttttcgagt ggttttgact
     8821 cgttgatttc gaagctgaga gcaccttcgg cggattcttc gattacctgt tcccctactt
     8881 ttgtttcttg agtgctgata ctaataatgt cggtgggttt atcttggccc cgtagttcgg
     8941 cgttgagagc ttgtgattct tcggacgtaa cgatctcgat tgcaacgtgc cgttcgccaa
     9001 tctcgcctaa ttttgtcgcg gcagctgcca atcctttttg tatagttgtg tcatcaatgt
     9061 gatggttggt agagttctgg aggacaaagt tgatcatatg gttactgtag aggaatgtcg
     9121 catttctcgc aaccaaatag gtctaaattc taccacggca tagcgcatta cattttcttc
     9181 caaggggagc cggcttgatc ccactatttt tatactggta cacatattgt atttgcttgc
     9241 tacgaaggct actatataac tagatcggga ggtgattgat tatgaaaatg tccacacttg
     9301 attgggttgc ctacatcctc gtggtgattg gcggtattaa ctgggggtta gtcggtgctt
     9361 ttgactacaa ccttgttgat tcgatctttg gcgacggttc ttctatcgcc cgcattgttt
     9421 acgttcttgt tggtctctcc gctctctact tgctcgccac ccttggccgc atgcagaagt
     9481 cataacatgc tatctttcca acacgaaaag acgcgtcacc gcgtcttttc gattatgaaa
     9541 ttttagttac tcgtcttctt tttcagcttg ctggcgcata aagtcttttg tgatcgtcgg
     9601 gatctgttct gctaaccagg ctgccatgtc ttcttcttcg ccgaggatcg tctggcacac
     9661 ttgttttgtt tcgtcatctt ccatctcggt ggcggctgca atcaaggatt gatacgaggc
     9721 aatctcgaaa tgttctgcgg tataatcggc taaggcgttt tttacaactt catccttata
     9781 catacctgta gaagcggctt tcattttgcc gagcatgctg ccaaatgttt ctttgccgct
     9841 cgacacatct tcgccgttac gttcaatgca ggctttgatc atttcggcgt gtttttttgt
     9901 ctgctcaata tgttcctgga ttttctcctg aacttcggtg tttccctcgg catctttctc
     9961 gtggttttcg agtacgggca cgaggccaac ttccatactg taagcatcgt taagccatcc
    10021 gatatatgtt tctttatgca tggtgttcct ttctctttgc tcgagcatag aaggtcggag
    10081 aataaaaaac attggggcag tctcctaaaa atatgtggta ttgagagaac caatcggaga
    10141 aatgtaaaac cctggcgctg ctccgtgcga gcgccagggt tttgtgagtt gagggcgtgc
    10201 ccctctagat agtgaagcgg cggcgtgctt cctgtgctcc tttaagcgcg tactgcggac
    10261 cccgggggtc gacgcagacg aagtggccca tcttccttcc ttcgcgcact tcgtccttcc
    10321 agtagtcggt gaacgacacg ccagggattt ggagcgcatg ctccaagccc tgaggattgg
    10381 cgttggcgtt tcgattgcct agtacattga ccatggccac gccgttgtgg aggtgccgtg
    10441 agttgggtcg tcctgctggt agtcctacca cacagcggac atgctgctcg aactgggacg
    10501 tttcgcaccc ttctactgtc aggtgtccgg cgttgtgaac gcgcggagca acctcattga
    10561 tgaagatgtc gccttctcca gtgacgaaca tctcgatcgt gaatacccct cgtccttcca
    10621 ggtgctccat cacttttcgt cccaggttag ttgcgcgccg gtcgaggttc gcatcctgat
    10681 gtccgagcat catggcgtgg tgacagatgc cttcctgctg aaaggtctcg attaccggat
    10741 agtgtcccac ggtgccggtg tagtcgcggt acataattac cgcaatctcc cgtgccacgg
    10801 gaatcgcttc ctccagatag aggtgctgat cacctacctt acgcactgcg gtcttgatgt
    10861 cctcgtcggt ggatatgaaa gccgtcccgc ggccgtcata tccgccgaag cgggttttga
    10921 caaccagttt ctgagtctcc cacgccttac gggctgcaaa cacggctccg ccaaggctcc
    10981 catctacggt catgatccgc ggtgtgggca agtcgtgctc aagccacatg cggtgctgcg
    11041 tgtacttgtc acggatcaac gccagcgcac tgggagcagg gttgatttcg tagcccgtag
    11101 ctacgagctg ctgaagtgtt tccacgccaa catgttcgat ttccacggtc acacccgccg
    11161 tccctcgatc ggcgagctgt cgcagccggt tggtgtcggt gaactttccg actatttgca
    11221 tggcgccagc atgggtggct gggcaatctt ggttcggatc gagtacaacg accggaatgc
    11281 ccagacggtt ggcggcaagg gtcagcatga acccaagttg acctccgccg ataaccccta
    11341 acatcttttt catgtttttc cctctgcaaa cagaaaagag cctacaggcg ctcttttaaa
    11401 gaattataat atagacctct gctctgatca agtagcagta ttaaaacgga ttgcgtggtt
    11461 taccgttaat gcgtaattca aagtgtaaat gcggtcctgt ggaacggcct gtattgccgg
    11521 aatagccaat cacatcacca cgtttgacga cttggccttc gcttacaata agtttgctta
    11581 agtgagcata gcgggtgttc tggctctcac tgtgggtaat tacgattgtt ttgccccaac
    11641 caccattcca gccgctggcg tcggcagttg taactgtgcc gtcagccgcc gcccagacgg
    11701 gtgttccaac ttcggctaag aaatcgatgc ccgtatgaac accggtaata ataccgcgag
    11761 aaatccctcg gtacttaatc ggcagcatat agcccgaagc ggaaatgtga gtatctgcgg
    11821 cttgggcttt ggcgatgatc gtattgcgtg ctgtattccg agcaacgttg ctgctgcttg
    11881 aagtcgttaa cggaacggct gttccttcgc gatactgaaa gaagtccgca gagtcttctc
    11941 gggtggtagt gcttacgccg cttaccgaag ccgtgacagg cgcttcgaaa gattgagcgg
    12001 gttgcgggat gagatttttt tcaatatagg ccgccaaaag cgttgggctg accataccga
    12061 tcagggacgc aattgtgagg ctgacaagga gataattggc tgctgccaac actctcgccg
    12121 gggttattga atgcatggat gcattttaca tggttaaaag tatagagtca accttttggt
    12181 gggtcggatg agctactttt aataaaaaag agtcgcctgg tgtatgtcga gcggctcgtg
    12241 ttttgtcact acaaaggacg ctctggggag cgtcctttgt agtgagtttt ttattttatt
    12301 ggagtttctg tccaacgagc tgagcggctt tggcgctgtc ggcggggttt tcgagtttgg
    12361 ctttgatggc ggccatcact ttacccatat ccgcgcgggt ggttgcgccc gtttcggtga
    12421 tgatttcgtt aacgagtgac tggagtgctt cgtctgagag gcctttgggc atgtattctt
    12481 caataatcgc cagttcggct tcttcttttt ccgccagctc gggccgaccg gcttctttat
    12541 attgagttgc cgcatctttg cgctgtttta cttcacggtt taaaacggca agcgtttgtg
    12601 gatcggtaag aacggtgcca aggccaccaa ctttaatggc ttcgttttta attgcgctgg
    12661 agaccatgcg taataccgcc acacggtcag cgtcgcgttc tttcatagca gcaatagtat
    12721 ctttttgaat ctgttcggcg agagtcatat gcctcctatc ctttcgcccc acacagagtg
    12781 tgtatgggga cgaaatgata cgaagttaat cttcgtgctc gggtttaata accgcgagtt
    12841 atgcgtttac ggtcagcttt acgctgtgcg atagtacgct taagtttgcg ggaagtatcc
    12901 ttttcgaaga agcgtttacg ctttgcttcg gtgaggatgc cgctctgctg tacttctcgg
    12961 aagaagcggc gcagaacaac ttcaaagttt tcacggccgc gtggacggtc cgatcgatcg
    13021 gagttttcac ggctgcgtgg acgaaagtcg tcagacaaaa caatcacctg ccttcaatgt
    13081 ctatttataa atgaataaca gactcattct agcatatatt ttctagattg tgaagttttt
    13141 agccggcatg ccgccggcta gcttgcgcta gcgtctcgct agctccgggg taaaagccta
    13201 tggtcacgtg cgaggccaag aaggtgtgtt ctcatcatgt ttgtctttgt atagcgtctc
    13261 ctagctatta aatagagtct ttgtttaata attataaaaa aacttgtgca atatgttgcg
    13321 ttattagtat ttgtgccgaa gtatcttgcg tggtataagt aagtacggct cgcagtgata
    13381 ggagtagtta accccgagag acatgcgtct ctgttctctg ctcctatcac tgtggctgtc
    13441 ccctttcccc tcccctctgg cgcttttgcg ttatgggagg ttttgtttta ctttgacacc
    13501 attggaccga tggcctctcc accgagaata tggacgtgga gatggtcaac ttcctgccca
    13561 ccatgagtac gggtattaat aatcgtgcga tagccagatg aggcaattct ctgttcctcg
    13621 gcaacctttt ttaccgttaa cataaccatc ccgaggagtt cttggtcttc gtcctccgca
    13681 tctgcgagcg attggatatg ttttttcgga ataacgagaa cgtggacttt agccttgggg
    13741 tgaatatcat taaacgctat cacgttttcg tcttcgtaga cgatgctggc cgataagtcc
    13801 ttgtttgaaa ttctgcaaaa gagacacggt gattcgtggg gcataggttc tccttatagg
    13861 tgctttacgg cgggtggtgc cgatggtttg ggctaaataa ttacgccagt tggctttaag
    13921 ccaagcctcg gattcttcgt agacaatggc taagttacgg ctgcgaagtt ctttttcgat
    13981 aatgtttcct cggtgagcat gaggatattt ttgcggatgt gggctacaaa atccatggca
    14041 ataatgcgaa agttcatgcg caattgtttg ccagatgacg ctctctggta cccgttcatc
    14101 gcgcattaac gaattaatcc gaatctccga acgggtcggg ttgtctcggc tgccgccttt
    14161 gccggtgatc gagcctaaac gattgcggtt cgttcttccc cactcgatgg ttacaatatt
    14221 aagccgttca atatcggaaa agtgctgact ccaaatgata tccaactgct gctggagcca
    14281 ggtgtggcta cgcttggttt tcgccgttgg attttcgttt gggctgtcgg tcgagtgatg
    14341 cgtcataata ggtatggtgt ttcacgttat caggtaaaaa ctgctgattc ttaaattgct
    14401 caggatcatt tttttcgtag cgcacgtagc ctttgccggc tcctagatct ttcataacct
    14461 ggttggcggc attgcgcaaa tgcatcggga ccggcccaat gggcaggttg tcgatatcat
    14521 agtatgcagc gtagagagcg tccataggtg cagtactttt cggggcattg gccaagtagg
    14581 ttacgccttg agctaaggtc aattgcgcct cgggcattcc tataagatga actgcctg
//
LOCUS       DASWYB010000029        15644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_833174, whole genome shotgun sequence.
ACCESSION   DASWYB010000029 DASWYB010000000
VERSION     DASWYB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15644
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833174"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..500
                     /locus_tag="VGE59_01630"
     CDS             234..500
                     /locus_tag="VGE59_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY1074382.1"
                     /translation="MDLVIILLVGALIGWVASLIMGTDQQQGAIANVLIGIVGAFLGR
                     WLFADVLGIGGATAAGALSLSGLFWGIVGAILLIGILRAVKVLR"
     gene            522..776
                     /locus_tag="VGE59_01635"
     CDS             522..776
                     /locus_tag="VGE59_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY1074383.1"
                     /translation="MPRQNKQKSSEGGMTVREAGKKGGNAVKQKYGAEFYQEIGEKGG
                     EARKRQLGSEGYRELGQKGGQRVKELIEQAKRAQGRKKDK"
     gene            1022..1417
                     /locus_tag="VGE59_01640"
     CDS             1022..1417
                     /locus_tag="VGE59_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY1074384.1"
                     /translation="MPRKNNANTGNREGGDMTVREAGRKGGETVREKYGPEFYQEIGE
                     KGGEARKQQLGHGGYVEMGRKGGEAVKQKYGPEFYEQIGRKGGEAVREKYGPEFYEKI
                     GQKGGQRVRELIEEGKQSGPSQRSKRSSK"
     gene            1451..1675
                     /locus_tag="VGE59_01645"
     CDS             1451..1675
                     /locus_tag="VGE59_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008959991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein"
                     /protein_id="HEY1074385.1"
                     /translation="MAEKKNGRGWHGDPAGHAKAGKKGGETTSQRFGREFYEKIGEKG
                     GEASGGKFEKGSERAKQAGKKGGESRAKNQ"
     gene            complement(1721..2431)
                     /locus_tag="VGE59_01650"
     CDS             complement(1721..2431)
                     /locus_tag="VGE59_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074386.1"
                     /translation="MIETRDLHAILISACKKLERDFAYEELGARLDTIRVKIVDAKEL
                     SHTLCGRKERRFYHVPAYYDPNKHVIYLNETMLFQENDTTVFIICYHELLHAISHHRN
                     YQEENTNIFQSGAKIEQFSYQFYNCSNRLLNEGIVQFLTNYYNNADDNAYAYSSETKL
                     IRKAAQLIGMTPFKRALLFGNLDSLESAFDGLFGAYSFRSFSKLLDKKEYAHAEALLT
                     PLSVMVLSPLLQESLSSA"
     gene            2605..3765
                     /locus_tag="VGE59_01655"
     CDS             2605..3765
                     /locus_tag="VGE59_01655"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074387.1"
                     /translation="MQISQTLAQEIREYIKNLLSKEVIIVDSVGKVIPSPGEADTSGF
                     SLPRDAIANSDLKTIDINGGPKLLIPLKYQAETVAALLVNDTSHDMASYVPLIRSFAE
                     LLVQQYLEVNKPVLDSTDQFISKLLYHATPAEYPFYESESRVLGYNVAARRLAIVIHL
                     ENFWNNCLASFDQPSFEREEIIRDWKRKIENNLTTFFTKATDNIVAYIGNDKFIIFKG
                     MEGEDDENIIKLLKKSHKAIFEPLKNFHIQEISVGFGNVAAGIGGMLSSYREADLALE
                     FGARLWGTNKSYYFGDLGILTILGEGNREKEILFANQLLHKLTNDDLVKTLECFFEQN
                     LNLTETANEMGIHRNTVIYRLNQISSILGVDPRVFEQAMTIKIALLIKKLFG"
     gene            complement(3823..4212)
                     /locus_tag="VGE59_01660"
     CDS             complement(3823..4212)
                     /locus_tag="VGE59_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1074388.1"
                     /translation="MNKDNFRKRVLIVEDDQMLVEMYKDKLKLEGFRVSTATDGKKAV
                     ARLKEGADIILLDILMPGLNGFEVLKKIKGTDETKETPVIVLTNLGNHLSENDKNLAL
                     SLGATDYMIKSLNTPDQVVERIQEILA"
     gene            complement(4199..6676)
                     /locus_tag="VGE59_01665"
     CDS             complement(4199..6676)
                     /locus_tag="VGE59_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY1074389.1"
                     /translation="MTDLLRIKTIRKFFEFPDFRLFADSFSHGLMVMNEEGRISFINK
                     KAQDICFSKETSQTPFELYSELIDPENTAMLDLIQDVLEFKRNIYRQVIHKQNTYLAE
                     AKVVTNGTKIIGSTILLSDITATAMKERQHELLYKISTALSQVSNVEQVLDTAIAQIT
                     GSVDIGAANIMILDKKTHKLRIVMDSNAPDRTIVPREIEMGEGIAGKCAQEKRPYSVY
                     DVAKSELYSQKRKNDRGALLAIPIISKGEVFGVLNITDPKPRYFTEGEVQFLTIIANE
                     IAVAIENSRLYERLNRRINQLSQMFYISSFGEVKAVDTRLQKMAQNITDLLDAEGCGI
                     YVYSATNQRLNLKYYSDHGTRLPSHIFINDEPLIKNAMESQTSVILNDKQNFPAESTL
                     NRRDIQSVLIVPIFIRQQAIGILKVFNKNEGPFDSDDENLAMITAHRIGTKIENAQLM
                     RRLESEKELLDKIIENTSEGVIVIDRKKKILIWNRYIEELTGLKKQEVVGQPCHKVLF
                     SRLGLKKLTQDIYTGNEIHHKLNEVHTYEQEIKNTRGEKIWISAVYSFILDEHRNVEN
                     TILVMRNTSKDKELLQAKDEFVSITTHELRTPLTAVKGYLSMILKGDAGEVTPKQNEY
                     FGKAYAATDRLSKLVEELLYVSRIDEERMTYTFTTFPIHDLIQECVEEMSQAAKAKQI
                     RITYSSQAAFIVRADYVKTKQVIENLIDNAIKYTRNRGQITITLEKRTREITLSVKDT
                     GVGIPAKHLASVFDRFVRIPNPLSIKAGGAGLGLYIVKNLVEDQGGKIWVASQPGKET
                     IFSFTIPLAQATPSGEKLERKENHEQR"
     gene            complement(6673..7230)
                     /locus_tag="VGE59_01670"
     CDS             complement(6673..7230)
                     /locus_tag="VGE59_01670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1074390.1"
                     /translation="MSFQCLACDSDAIQEVVQDNYRFFYCSTCEKLYERASNTDYGKD
                     LIVNTKKGIVHVCVGALIRQNNKFLLIKRRAYPFGYGFPAGHLEYEEQPQEGLAREVF
                     EETGLRIKNSELLFNDFIPTSKCRYGADNHIWYFYAVECEEGVPVLNPESEGIGWFSM
                     EEAKNLNLIPSAQHLLTHITFPDPQ"
     gene            7414..8373
                     /locus_tag="VGE59_01675"
     CDS             7414..8373
                     /locus_tag="VGE59_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074391.1"
                     /translation="MLPNTHREERYERLCRLYCNPQLLAVAQDVQFYSNKEFLSRISE
                     KFPEPVRNAFAWRTTNWAGVYSKGKTTNLRAAMLHLQSEGIKVGFVPEVTVGRQIRPG
                     EVAGEFVYPMDREDFRRERRNGNMLVAYWRGRGTHRYKLGGIWLPLVEKALAEYDVLF
                     TNINSAALYWMKSHFAPFLASVVVVTDEEAHGMMQFMRNVPPNDSEGMNPLGVRGEFP
                     ILSQEELPDVLWWENELHPEAQGFTDERVRQEYLIGLLSQRFNQPLKPFECGNFPLEL
                     PVRLLPRTRGEYLRMLVMRGEHCIPSHVLREPDVWCCDIQMAA"
     gene            8487..8560
                     /locus_tag="VGE59_01680"
     tRNA            8487..8560
                     /locus_tag="VGE59_01680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8519..8521,aa:Gln,seq:ttg)
     gene            8764..9354
                     /locus_tag="VGE59_01685"
     CDS             8764..9354
                     /locus_tag="VGE59_01685"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY1074392.1"
                     /translation="MILPPDFYALPTELVAQELLGKLLVHESPEGITVGRIIETEAYV
                     SNDPANHAYRGQTQRNAPMFGPAGRSYIYLIYGMHYCFNIVTAPQGVGEAVLIRALEP
                     LEGIPLMQLRRNKVNLSELTSGPAKLVQAMGVTLSQNNKDVTSPPLYVADQYRSHCAL
                     EKNSFSFIAGPRVGIRVGTDLNLRYLIVPEGRSPLA"
     gene            complement(9320..9757)
                     /locus_tag="VGE59_01690"
     CDS             complement(9320..9757)
                     /locus_tag="VGE59_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074393.1"
                     /translation="MTKPQLVSTGMIIIVLALGIGYATKKFAYARESKNRSAIMLTNG
                     RTYIGYLSQRNNQYVTLDEAYYITPNSAPDTEPKKKLTLTKVSDEAYGPEERIYINRD
                     TIVSIAPMRNESKVTSAITKYEQEHQASPSPSITPAETVPPAQ"
     gene            10049..10993
                     /locus_tag="VGE59_01695"
     CDS             10049..10993
                     /locus_tag="VGE59_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074394.1"
                     /translation="MYKRAYSIRKCGLYHSGLGLLLSGAMLLPGFGLAASDLENTTTG
                     SESVNQNSVNETRDTTVTETNTSNTTNLATFNFRTGGNTVQENTTVGDFTTGSMRIDY
                     TAQTQANDTPNVSANSASVVTEDISSANTRTGAGSENINQVTSSQSATLTKSQIANVV
                     NEVTLVASTGDNLVARNTTVGDITTGDIDARVRFITRANTSVTPVTPVSPSPTPGGGG
                     GSNPVGGGSPSTPSSSTTTTHPVTLTAILPTGMGGAQEVMPFSLTQSSRKFFPAGSTQ
                     TPYLLILALLGLWLISPSAIQRLRTSYSLISRSENKMK"
     gene            11087..11965
                     /locus_tag="VGE59_01700"
     CDS             11087..11965
                     /locus_tag="VGE59_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074395.1"
                     /translation="MTHIDIIKVRNMMRTIAAASAVVLSMGAGSVFAQIDVSGTNGTT
                     GANSVNENMYDVDADVDVDIDNTADTTNVAVADVDAGANDISMNTTVEDITTGDVIGE
                     VSFDTELNSGGSLSLDSLMMQDVSVDSSNDTTGANSENTNDVDVDSDVDIDITNTADV
                     SNVLSATVRTGGNAVRQNTTVGDVTTGDTIISGDFTTEVNSNTGVDLGNMSAPSVDAS
                     FSNDTTGFSSSNVNTVDVDSDLDVDVTNDATVNNTATVSATPGNNAVTGNTTVGNIHT
                     GGVRFEVNSTTTANSQ"
     gene            12033..13982
                     /locus_tag="VGE59_01705"
     CDS             12033..13982
                     /locus_tag="VGE59_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074396.1"
                     /translation="MKQFSKRIYSFIAGALLVIFSTFPIVSVWAVGPTEPVGPQGPVG
                     PIEPVGPQSPVGPQLLVSPTPSSSVEVVSTAAESSGAPVTEQQNVQTGSGSENNNNLA
                     ATSNTTATQNNTSTIDNTVGIQASTGGNDISRNSSVGDITTGNIAGTVTIVNAAQSQF
                     AAGSSIGTQTVSQVGTDPLVLNPASSRTSLQSNDTTGSDSENTNVSNSSDTTIITTNS
                     TADANNSIVIDASTGGNKILQNSEVGDILTGDINLEVNLINLLNVYWPGVQLALDVWS
                     VPGGLNGDIVIPDQGSTNTGSNSSNTNAVTQESTATINVVNNADISNDITVDATTGDN
                     DISQNSKVGDITTGETNVTGNITNVSNVGQPIVYLINVFGQWMGSLLGLDGQQVIVNQ
                     MNDTTGPNSDNSNTSTQTSNTTLNATNTARANNTVQVHANTGENTITQNTEVGDITTG
                     SVNVYANVVNILNSWSDHVGSFALRIVNVWGNWNGNARGNQPLASSGSTPVSQSSAIE
                     TQPIPLPSASPDPHEGMQIAALHAVGIATPPSFAPSTNKEEKISTHTRLRTYISQFTL
                     TSLDSNNVTPDDTSSPNNPSVQVPNEQVSATVSAQNPEKEASSEVKPKQAQVLSGGVV
                     TASVLAFLVLGMWLGVEYVSSKKEK"
     gene            13992..14606
                     /locus_tag="VGE59_01710"
     CDS             13992..14606
                     /locus_tag="VGE59_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEY1074397.1"
                     /translation="MVTTRIPGISISLALLSLSVWAYPFLSATLRPHLIETVPSVVLA
                     PAVSKVSATSVSQVVIPDLGLTAPVTFLPHIDPLEVQDWNAIQPVLRDGVALTTTDES
                     FDSAASSFLIGHSSDYVPNKYSFIFAGLNALKVGDVVSLTLKGKTYEQTVVEKKILSP
                     DDSEAFADLMTPVIGKQRLFLVTCWPVFTTKQRLVVITERTHTE"
     gene            14660..>15644
                     /gene="rmuC"
                     /locus_tag="VGE59_01715"
     CDS             14660..>15644
                     /gene="rmuC"
                     /locus_tag="VGE59_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY1074398.1"
                     /translation="MITGILIGAGIAGLVCVFFLIWQKRQQPNLEASFEQHAQKALGM
                     MMQEVIKASREQLGAEKREISETIAKDLQTQTNTFKEVAQSLRIEIDARQREIRQLEE
                     DRNKKYGQISQALTDYKSLTEELRGSTEQLKRVLSSNQQRGSWGEMQAEKILEAAGMV
                     AGQHYAKQQPIEGFTGLRPDFTLFLPNKMELYVDVKFPLTALQEVYSSTTKEDQERYF
                     KRFGDDLRDRIKEAGKYVIDKSNCVDYVILFVPSESVFEIINKHFPAIVDASFSQQVI
                     LVSPHSFFAVVRTIQESYTHFYYEQNLKEILGYLQQTLQQFERFKGEFADVG"
BASE COUNT         4249 a         3249 c         3772 g         4374 t
ORIGIN      
        1 tggttagctc cttttttttg tatcctgtgt tagttataac ttacaaaatt aaaaaatttg
       61 tcctagggtt accttgtttg ttcggccgat gaagcggtaa cgggcatgcg ctattgttgt
      121 gagcaagaag acagaagtgt cttaagcgct taagccaaac cccgggagga ggtgaaaata
      181 aaaattgcgc tggcacggat tcttcaaaat ctatgctttg agaaaggagc actatggatt
      241 tagtcattat cttacttgtt ggtgcgttaa ttggttgggt tgcaagcctt attatgggaa
      301 ctgatcagca gcagggtgca atcgcaaacg tgttaattgg tatcgttggt gcatttctcg
      361 gtcgttggct tttcgctgac gtactcggaa tcggtggtgc aactgctgcc ggcgcactga
      421 gtctctcggg tcttttctgg ggaatcgtag gcgctatcct cttgattggt atccttcgcg
      481 ctgtaaaagt cttgcgttaa gacgccggca aaggagccat catgccacga cagaataaac
      541 agaagagctc ggaaggtgga atgaccgtac gtgaagctgg taaaaaaggc ggcaatgcag
      601 tcaagcaaaa atacggtgcg gaattctacc aagagattgg cgaaaaaggt ggcgaagctc
      661 gaaaacgtca acttggttct gaaggatatc gtgaacttgg tcaaaaaggt gggcagcgcg
      721 tgaaagagct gattgagcaa gcaaaacgtg cgcagggacg taagaaagat aaataggaat
      781 ttcgctacca aagaggaaag aacccggcta gtccgggttc tttctatggt gtactttttc
      841 tgagaagagg acgagtattt ttgcgtttct atgactcctc caatttctcg gccacagggg
      901 tgagcatttt tggttacatc cgccgatgaa ctctccttct ttgaaaacta tgatgtggct
      961 acaaggtcga cagaggaagc acagagaaaa acgaatgtgc ttaaggtagg aggtgaaagc
     1021 aatgccacgt aaaaataatg ctaatactgg aaaccgcgaa ggcggcgata tgacagtccg
     1081 tgaagcaggt cgtaagggcg gtgagaccgt gcgagagaag tatggcccgg agttttacca
     1141 ggagattggc gaaaaaggcg gcgaagctcg caagcagcag ctcggccatg gtggatacgt
     1201 tgaaatgggc cgaaagggcg gagaggctgt taaacagaag tacggtccgg agttctatga
     1261 gcagattggt cgtaagggcg gtgaagctgt tcgggaaaaa tatggacctg agttctacga
     1321 aaaaattgga caaaagggcg gtcagcgtgt tcgcgagctg atagaagagg gtaagcaatc
     1381 tggtccgtcg cagcgttcca agcgctcgtc aaaataatat ttctcacgaa gtaaaaagac
     1441 ggaggctact atggcagaga aaaagaacgg tcgtggttgg catggggatc ctgctggtca
     1501 cgccaaagcg ggtaaaaaag gtggcgaaac aacatctcaa cgatttggcc gtgaattcta
     1561 cgaaaaaata ggtgaaaaag gtggtgaagc aagcggcggt aagttcgaaa aagggtctga
     1621 acgagcgaag caagccggta aaaaaggcgg cgagtcgcgg gccaagaacc aatagatcac
     1681 atttacgaaa agcccggtaa taaaccgggc ttttcgtgta ctaggctgaa gacagcgatt
     1741 cctgcaacag gggcgagaga accattactg acagtggggt aagaagtgct tccgcgtgtg
     1801 cgtattcttt cttatctaaa agtttggaaa aagagcgaaa actatatgcg ccgaatagtc
     1861 cgtcgaaagc cgattctagg ctgtcaaggt tgccaaagag cagagcacgt ttaaaaggtg
     1921 tcattccaat caattgggcg gcttttcgga tcaacttcgt ttcactgctg taggcgtagg
     1981 cgttatcgtc ggcattgtta tagtaattgg tcaaaaactg tacgatacct tcattgagaa
     2041 gccggttact acaattatag aattggtagc taaactgttc gattttcgct ccggattgaa
     2101 aaatgttggt gttttcttct tggtaattgc gatgatggga aatcgcatgg aggagctcat
     2161 ggtagcaaat gataaaaacg gtggtgtcat tttcttggaa gagcattgtc tcgtttaagt
     2221 aaataacatg cttattggga tcgtaatagg cgggaacatg gtaaaatcgt cgttctttac
     2281 ggccgcagag ggtatgagac agttctttcg cgtcaacaat ttttacccgt attgtgtcga
     2341 gtcttgcccc cagttcttcg taggcaaaat ctctctccag ctttttacag gccgaaatca
     2401 ggatagcgtg taggtcgcgt gtctcgatca ttggttccta ttcttattca cgtagcgggg
     2461 tgttgtcaaa ttttacgaaa aactgtgagc gatcacagtt aaatttgtat gagtcttcta
     2521 actttagaat caccccgacc tattataatg atttgtatag tattacaaaa acggtataat
     2581 acccgtaacg aggaggctac gtttatgcaa atttctcaaa cactcgccca ggagattagg
     2641 gagtatatta aaaatctcct cagtaaagaa gtgattattg tcgactcggt tggcaaagta
     2701 attccttctc ctggcgaagc tgatacctcc ggcttttctt tgccccggga tgctatcgcg
     2761 aatagcgatt taaagaccat tgatattaat ggcggcccta agcttctcat tcctcttaaa
     2821 tatcaggcgg aaactgttgc agcattgctt gtaaacgaca cctctcacga catggcaagc
     2881 tatgtgccgc tgattcgtag ttttgcggag ttgctcgtac agcaatatct ggaagttaat
     2941 aaacctgtcc ttgactctac ggatcagttt attagcaagt tgctttatca cgcgacacca
     3001 gcggaatatc ccttctacga gtcggaatcc cgtgttttgg gctataacgt agccgcacga
     3061 cgtttagcaa ttgttatcca tttagaaaac ttttggaata actgtttggc gtcttttgac
     3121 caaccaagtt ttgaacgcga agaaattatt cgagactgga aacgtaaaat cgaaaataat
     3181 cttacgactt tctttaccaa agcgaccgac aatattgtgg cgtacattgg gaacgataaa
     3241 tttatcattt ttaaggggat ggagggggaa gatgatgaaa atatcatcaa attacttaaa
     3301 aaatcgcata aggctatttt cgagcctctt aaaaacttcc atattcaaga aataagtgtt
     3361 ggctttggaa atgtcgcggc aggaattggc ggtatgctca gttcgtatcg tgaggctgac
     3421 ttggctctgg agtttggggc tcgcttatgg ggaacgaata aaagctacta ctttggtgac
     3481 ctgggtattc ttacgattct aggcgaggga aatcgcgaaa aagaaatcct ctttgctaat
     3541 cagttattgc ataagttaac caatgatgac ctggttaaaa ccttagagtg tttctttgag
     3601 caaaacctta atctcacaga aacagccaat gaaatgggaa ttcatcgcaa cacagtgata
     3661 taccggctta accagatatc cagcattctt ggtgtcgacc ctcgggtttt cgaacaggcg
     3721 atgacgatta aaattgcttt gctgattaag aagctattcg gataaacacg taaaaacaag
     3781 taatacaaaa gagcccctta tgcgggggct cttttgtgga gtttaggcta atatttcttg
     3841 aattcgttca acgacttggt cgggggtatt aagcgactta atcatatagt cggtagcgcc
     3901 tagtgaaaga gctagatttt tatcgttctc tgataagtga ttgccgaggt ttgttaaaac
     3961 gataacgggc gtttctttag tttcgtcggt ccctttaatc tttttaagaa cttcaaagcc
     4021 attgagtcca ggcataagga tgtctaataa aatgatgtcg gccccttctt tgaggcgggc
     4081 aacagctttt ttgccatcgg ttgccgtgga aacacgaaaa ccttcaagct tcagtttgtc
     4141 tttatacatc tcgacgagca tttgatcgtc ttctacgatc aggacacgtt tacgaaaatt
     4201 atctttgttc atgattttcc tttctttcga gtttttctcc acttggagtg gcttgtgcta
     4261 agggaatagt gaatgagaat atggtttcct ttccgggctg actggcgacc catattttgc
     4321 caccttggtc ttcgacaagg tttttaacaa tgtaaagccc aagccccgct ccacctgctt
     4381 tgatactgag tggattagga atgcggacaa accgatcaaa gacagacgca aggtgtttgg
     4441 cagggatacc aactcctgta tctttgacgg aaagcgttat ttcgcgagtg cgtttttcta
     4501 gcgtaatcgt aatctggccg cgattgcgcg tatatttaat agcattgtca ataagatttt
     4561 caatcacctg tttcgttttg acatagtcag cgcggacaat aaaagcagct tggcttgagt
     4621 aggtaatacg aatttgcttt gctttcgcag cttgtgacat ctcttcgacg cattcctgaa
     4681 tcaggtcatg aatagggaaa gttgtaaagg tataggtcat gcgttcttca tcgatccttg
     4741 agacgtaaag gagttcttcc acgagtttag agaggcggtc ggttgccgca taggctttgc
     4801 caaaatactc attttgttta ggcgtgacct cgccagcgtc gccttttaaa atcatgctca
     4861 agtaaccttt tacggcagta aggggagtgc gaagttcgtg cgtggtgatc gatacgaatt
     4921 catctttcgc ctgaagcagc tctttatctt ttgacgtatt tctcatgacc agaatggtat
     4981 tttctacgtt gcgatgctcg tcgagaatga aagagtatac ggcggaaatc catatttttt
     5041 ctccacgagt atttttgatt tcctgttcgt aagtatggac ttcgtttaac ttatgatgta
     5101 tctcattgcc ggtataaatg tcttgggtaa gcttctttaa cccgaggcga ctaaaaagta
     5161 ctttatgaca aggctggcca accacttcct gttttttgag tccggtaagt tcctcgatgt
     5221 atcggttcca gataaggatc ttcttttttc ggtcaatgac aataacgccc tcactcgtat
     5281 tttcaatgat tttgtcgagc agttcctttt ctgattctag ccgtcgcatg agttgggcat
     5341 tttcaatttt ggttccaatt cgatgggcgg taatcatggc gaggttctcg tcgtcggaat
     5401 cgaaagggcc ctcgtttttg ttaaatacct ttaatattcc aattgcttgt tgacgaataa
     5461 agatggggac aatcaacacg gattggatgt ctcgtcggtt gagagtactc tctgcgggaa
     5521 aattttgttt gtcattcaaa ataacagatg tttgactttc catggcgttt ttaatgagag
     5581 gttcgtcatt gataaagata tgcgaaggga gacgggtacc atgatcgctg tagtatttaa
     5641 gattcagccg ctggttcgtg gcggagtaca cataaatacc gcagccttct gcgtcgagta
     5701 ggtcggtgat attctgagcc attttttgta aacgagtgtc gacagccttt acttctccaa
     5761 atgaagagat gtagaacatt tgcgagagtt gattgattcg acgattgaga cgttcgtaga
     5821 ggcgactgtt ttcgatggca actgcaattt cattggcaat aatggttaaa aactgcactt
     5881 caccttctgt aaaataacga ggtttgggat cggtaatatt aagtacgcca aagacctccc
     5941 ctttacttat aataggtata gccagtaatg cgccacggtc gttttttcgt ttttgactgt
     6001 agagctcaga ctttgccacg tcatacacgc tataaggacg tttttcttgc gcgcatttgc
     6061 cggcaattcc ttctcccatt tcgatctctc gaggtacgat agtcctatca ggcgcattgc
     6121 tgtccattac aatacgaagt ttatgagttt ttttgtcgag tatcattatg ttagcagcgc
     6181 cgatatctac cgatcctgtg atctgagcaa tcgcggtatc aagtacttgt tcgacattac
     6241 ttacctgcga taaagcggtc gaaattttat aaagcagttc gtgctgtcgc tccttcattg
     6301 ctgtggccgt aatgtcgctc agtaagatag tagaaccgat aattttagtg ccattggtaa
     6361 cgacttttgc ctcggcgaga tacgtatttt gtttatgaat cacttgacgg taaatattac
     6421 gtttaaactc aagcacatcc tgtatgaggt cgagcatcgc agtattttcc ggatctatta
     6481 actcagagta gagctcgaat ggagtttggg atgtctcttt gctaaagcaa atatcctgag
     6541 cttttttatt aataaaagaa atgcgccctt cttcgttcat aaccataagg ccatgcgaaa
     6601 aagagtccgc aaataaacgg aagtcaggaa attcaaagaa tttacggatc gtttttattc
     6661 gaaggaggtc ggtcattgtg ggtcgggaaa agtgatatgt gtgagaagat gctgtgcaga
     6721 agggatcaaa ttaagatttt ttgcttcttc catactaaac cagccaattc cctcgctttc
     6781 aggatttaaa acgggaacac cttcttcaca ttcaacagca taaaaatacc atatatgatt
     6841 atctgcgccg tagcggcatt tgctggttgg gatgaaatcg ttgaatagta attcggaatt
     6901 tttaattcta agcccagttt cttcgaagac ttctcgtgcg agtccttctt ggggttgttc
     6961 ttcatattct aaatgtccgg cgggaaaacc gtaaccaaaa ggatacgcac ggcgtttaat
     7021 aaggagaaac ttgttatttt gacggattag agctccgacg caaacatgca caataccttt
     7081 cttagtattt acgattaaat ctttcccata atcagtatta cttgctcttt catataattt
     7141 ttcgcatgtg gagcaataaa aaaaacgata gttatcttga actacttcct gaattgcgtc
     7201 gctatcgcat gctaaacatt gaaatgacat aaatctcctt acttactgta tcttaaacaa
     7261 attggattag gagtcgtttg gaataatgta catccctatg attattaaag ggtgcttgtt
     7321 tgtgataatt tacaaaaagt tgacgtttgg taagcaatgc atataattat agtgtctgac
     7381 atggtgtcag ggtaactgga aaatcaaagc aacatgcttc ccaatacgca tcgtgaagag
     7441 cggtatgagc ggctttgccg attatactgc aatccgcagt tgctcgctgt ggcacaagac
     7501 gtccagttct actcgaacaa ggagtttctg agccgaatta gcgagaagtt ccccgagccg
     7561 gtgcgcaacg catttgcttg gaggaccacg aattgggctg gggtctactc gaaagggaag
     7621 acaaccaacc tcagggctgc aatgcttcac ctccagtcgg agggtatcaa ggtaggtttt
     7681 gtaccggagg taacagtagg gcgacagatc cgtccgggtg aggttgctgg tgagttcgtc
     7741 taccccatgg atcgggagga ttttcggcgg gaacgaagaa acggcaatat gctggttgct
     7801 tactggcgag ggcggggaac tcaccggtac aaactcggtg gaatatggct tccattggta
     7861 gagaaagcat tagctgagta cgatgttctg ttcacgaaca tcaattcggc ggcactctac
     7921 tggatgaaga gccacttcgc cccattcctc gcttcggttg ttgtcgtaac tgatgaggag
     7981 gctcatggca tgatgcagtt catgcgtaat gttcccccaa acgatagcga agggatgaat
     8041 ccattgggag taagagggga gtttcccatt ctctctcaag aggaattacc cgacgtcctc
     8101 tggtgggaga atgagctgca tccagaagct caagggttca ctgatgaacg agtgcggcaa
     8161 gagtatctga ttgggttgct atcgcagcga tttaatcagc cgcttaagcc gttcgagtgc
     8221 ggtaattttc ctttagagtt gccggtgcga cttttgccca gaactcgagg agagtacctg
     8281 cggatgctcg ttatgcgagg cgagcactgt ataccaagcc atgttctccg tgagcctgac
     8341 gtctggtgtt gcgacataca aatggcggca tgagtcgcca aactgctcag gggcacctga
     8401 ggatgggacg cggatgaccg cgtcccattt tttattaaca atcttgagtt tgcacctgtt
     8461 tttcggtata gtgtataggt agttattccc ggatcgtcta atggcaggac agtggccttt
     8521 gaagccatca atctaggttc gacccctagt ccgggaacca acgagaagca ccccaattaa
     8581 aggggtgttt ttcgttggtt tctgaccagg agagaaccgt agagcggagc ggaaggttcg
     8641 atagtcgaag gcacgcgcag aagcgtgttt cgagcatgcc tgaagattat acaaagtacc
     8701 cctagtccgg gaaccataca aaaccccgcc tcaacggcgg ggttttgtga tagggtaaaa
     8761 catatgattt taccacccga tttctatgct ctgccaacag agctcgtagc ccaagagctt
     8821 ttaggaaaac ttctcgttca cgaatcgccc gaaggaataa cggtgggacg aattatagag
     8881 accgaagcgt atgtctctaa tgatcctgcg aatcatgcgt atcgcggtca aacgcaacga
     8941 aacgcaccca tgttcgggcc tgctggcaga tcatatatct accttattta tggaatgcac
     9001 tactgcttta atattgttac cgctccccaa ggagtggggg aggccgtatt gatccgagcg
     9061 ttagaacccc tcgagggtat ccccttgatg caattgcgtc gaaataaggt aaatctctcc
     9121 gagcttacaa gtggtcctgc aaagctggtg caagcaatgg gtgtgacttt atctcagaac
     9181 aataaagatg taacctctcc tcctctctat gtagctgatc aataccggtc tcattgtgcg
     9241 cttgagaaaa attcgttttc ttttatagct gggccaagag tgggtattcg tgtggggacc
     9301 gatctgaact tacgttacct tattgtgccg gagggacggt ctccgctggc gtaattgatg
     9361 gtgaggggct cgcttgatgt tcttgttcgt acttcgttat cgcgctcgta acttttgact
     9421 cattacgcat gggcgcaatc gacacgattg tatcccgatt gatgtagatt cgctcttcgg
     9481 gcccatacgc ctcgtcgctc actttggtta gggtgagctt tttctttggt tctgtatcgg
     9541 gcgcagaatt aggagtgata tagtacgctt cgtcaagtgt tacatactgg ttattccgtt
     9601 gcgaaaggta accaatgtat gttcggccgt tggtgagcat gattgccgag cgatttttgc
     9661 tctcgcgggc gtaggcaaat tttttcgttg catatcctat gccaagtgcc aatacaataa
     9721 tgatcattcc tgtgctaact agctgtggct ttgtcatata cctcctcgta tttcttcttt
     9781 tataacgtct tatgccccta atgtatttgt ttgtttcgct aattctacat gcgaagcaaa
     9841 cggtatatct ggaaatgcag ataaatagca tcttgtttag caagtaacta gccagtggta
     9901 tgtgtattcg tatatatttc aagctgctta ttcattacca caaaaattat gtgtctactg
     9961 tggggctttt tggtattacg tgtatagatt taaggttcgc catcgatcat attttaaatc
    10021 gacacaaaaa tactatgagg agtgggaaat gtataaaaga gcatatagca ttaggaagtg
    10081 tggtttgtat catagcgggc tcggactttt gctttccggg gcaatgcttt tacccggttt
    10141 tggattggcg gcaagcgacc tagagaatac tacgacaggc tctgagtcgg tcaatcagaa
    10201 cagtgtaaat gagacgagag ataccacggt aaccgaaacg aacacttcaa acactaccaa
    10261 cctagctacc tttaattttc gcacgggtgg gaacactgtc caggagaata caacagttgg
    10321 tgattttact actggtagta tgcgtattga ctataccgcc caaacgcagg caaacgacac
    10381 tcctaatgtc agtgccaata gcgcttcagt tgtaacggaa gatatttcat ccgcaaatac
    10441 acgcacaggt gcaggaagcg aaaacataaa ccaagtaaca tcttctcaat cggcaacgtt
    10501 aacaaagagt cagatcgcca atgtcgtaaa cgaagtaacg ctcgtggcct ctacaggcga
    10561 taaccttgtc gctcgtaata caactgtggg cgatattacc actggcgaca ttgatgctcg
    10621 tgtcagattt ataacccgtg caaatacttc agttacgccc gtaactccag tttcgccttc
    10681 tcctacgcct ggaggtggtg ggggaagtaa tcccgtggga ggtgggtcac cttctacccc
    10741 ttcttcctca actacaacga cacatccggt aacgctcacg gcgattcttc caactggtat
    10801 gggcggggct caagaggtaa tgccatttag ccttactcag tcatctcgta aatttttccc
    10861 tgctggctct actcaaaccc cttaccttct tatactggct ctacttggct tatggctgat
    10921 cagcccttcg gccatccagc gtctccgaac ctcatatagt cttataagtc gatcggagaa
    10981 taagatgaag taattacatt tgggtcgatg catcagagat acagtgtgca cctctgatga
    11041 atattaatag gcacgcagtc ccaagtattt taaagaagga gtggatatga ctcatatcga
    11101 tatcataaaa gttcgaaaca tgatgcgtac aattgcagct gcatccgcag tcgtgctctc
    11161 gatgggcgca ggatcagtat ttgctcaaat tgatgtcagt ggtaccaatg gaacgactgg
    11221 cgccaactcg gtcaatgaaa atatgtatga cgttgatgct gatgttgacg tagacattga
    11281 taatacggct gacacgacca acgttgctgt cgctgatgtt gatgctggcg ctaatgatat
    11341 ctccatgaat acgactgttg aagacattac gacaggtgac gtgataggag aagtctcgtt
    11401 cgatactgag ctaaattctg gtggcagtct gtcattggat tccctgatga tgcaggacgt
    11461 aagtgttgat tcgagtaacg atacaacagg tgcgaactcg gaaaatacaa atgatgttga
    11521 tgtagatagc gacgtagaca ttgatattac gaatacagct gacgtgagta atgtcttaag
    11581 cgcgacggtg agaacaggtg gcaacgctgt gcgccagaat acgacagttg gggatgtaac
    11641 aactggggac actattatca gcggtgactt cactaccgaa gtgaactcaa atactggagt
    11701 cgacttagga aacatgagtg cgccatcagt tgatgctagc tttagcaatg acacaactgg
    11761 cttctcctca tcgaatgtta acacagtcga tgtcgatagt gatcttgatg ttgacgtaac
    11821 gaacgacgca acggtaaata ataccgctac ggtgagtgct acccccggta ataacgcggt
    11881 aactggcaac acaacggttg gtaatatcca caccggtgga gtccgtttcg aggttaactc
    11941 tactaccaca gctaactcac aataagctga ggagtcagtg agggcctagc ttatgctagg
    12001 ctccacgggt ttctcacgaa ctaaggaggt ccatgaaaca attctcaaaa aggatatact
    12061 cttttatagc cggtgctctc ctcgttatat tttcaacatt tccaattgtc tcagtgtggg
    12121 ctgttggacc aactgagccg gtcggtcctc aaggtcctgt tggacctatt gaaccagtcg
    12181 gccctcagtc tccagtcgga cctcagttac tcgtatctcc taccccttct tcatcggttg
    12241 aggtagtttc tacggccgcc gaatcttcag gtgctcccgt gacagagcag caaaacgttc
    12301 aaactggatc aggttcagag aataataaca atctagcagc aactagcaac accaccgcca
    12361 cacaaaataa cacgtcgact attgataata cggttggtat tcaagcttcg acaggtggta
    12421 atgatatctc ccgtaacagt tcggttggag acataactac tggtaacatc gcaggtacgg
    12481 ttaccattgt taatgctgcc cagtcacaat ttgctgctgg tagcagtatc ggtactcaaa
    12541 ccgtttctca ggtcggtaca gatccccttg tattaaatcc tgcttcgagc cgtacttcgc
    12601 ttcagtctaa tgacacgact ggttctgatt ctgaaaacac gaatgtaagc aactctagtg
    12661 ataccactat tatcactact aatagtactg ccgatgcaaa taactcaatt gttattgatg
    12721 cctcaacggg aggaaataag attctccaaa acagtgaagt gggagatatt ttgaccggtg
    12781 atattaactt ggaagtgaac ctgattaatc tgttgaacgt atactggccc ggcgtacaac
    12841 tagccttaga tgtctggtcg gttccgggtg gtttgaatgg cgatatcgtg atccctgatc
    12901 aggggagtac taatacaggt agtaattctt caaatacgaa tgccgtgaca caagagtcta
    12961 cggcaacaat aaatgtcgta aataacgcag atattagtaa tgatattact gtcgatgcaa
    13021 cgacgggtga taatgatatt tctcaaaact ctaaggtggg ggatattacg actggcgaaa
    13081 ccaatgtaac tgggaacatt acaaacgttt ccaatgttgg tcagcccata gtttacctta
    13141 ttaatgtatt cggtcagtgg atgggctctc ttcttggatt ggatgggcag caagttatcg
    13201 ttaatcaaat gaatgatacg accggcccaa attcagataa ctcgaataca agtacgcaaa
    13261 cttcaaacac aacactaaat gcgacgaaca cagctcgagc aaataataca gttcaggtac
    13321 acgccaatac cggtgaaaat acaattactc aaaatacaga ggtcggagat attacgactg
    13381 gttcggttaa tgtgtatgct aacgtggtta atattcttaa ctcttggagc gatcacgttg
    13441 gaagttttgc tctccggatt gtgaacgtct ggggtaactg gaatggtaac gcccgtggca
    13501 accagccatt agccagttca ggcagtacgc cagtgtcaca gagctccgcc atagagactc
    13561 agccaattcc tctcccttct gcgtctccgg atccacacga aggaatgcaa attgccgctt
    13621 tgcatgcggt gggaatagct actcctccca gctttgcgcc ttctaccaat aaagaagaaa
    13681 agataagtac tcatacacgc cttcgcacct acatttctca atttaccctt acgtccctgg
    13741 attcaaacaa cgtcacaccg gatgatacta gctctcccaa taatccatct gtgcaagtgc
    13801 ctaatgagca agtttccgca acagtgagtg cccaaaatcc agaaaaagaa gctagctcag
    13861 aagttaagcc caagcaagca caagtcctat caggcggagt cgtaactgcg agtgttcttg
    13921 ctttcttggt ccttggtatg tggttaggag ttgagtatgt ttcatcaaaa aaagaaaagt
    13981 gaataaagta aatggtaaca acgcggattc caggtataag tattagtttg gcgctactgt
    14041 cactctcggt gtgggcatat ccatttttga gtgcgacttt gcgacctcac ctgattgaaa
    14101 cagtgccaag tgttgtactt gctccagcgg tatcaaaagt ttcagcgacg tccgtatctc
    14161 aagtcgttat acctgatttg ggacttactg cgccggtaac atttttacct catattgatc
    14221 ccctagaggt acaggactgg aatgcaattc agccagtgtt acgagatggg gtagccttga
    14281 ctacaaccga cgagagtttt gattcggcgg cgagctcgtt cctaattggg cattcgagtg
    14341 actatgtgcc aaataaatat agttttattt ttgctggcct taatgccctc aaggtaggag
    14401 atgtagtgtc tcttactctt aagggtaaaa cgtatgaaca gacggtggta gaaaagaaga
    14461 ttctttctcc tgacgatagc gaagcattcg ctgatcttat gacgccggtt attggtaaac
    14521 agcgactctt tttggtcacc tgttggcctg tatttactac gaaacaaaga ctagttgtca
    14581 taacggaacg gacgcataca gagtaatgtt ttctaggtta gggcttgtgt tgctgggcga
    14641 tattgggcat agtagccata tgattacagg cattctcatc ggggcgggga tcgcaggttt
    14701 ggtatgtgta ttttttctga tctggcaaaa gcgtcagcag cctaacttag aggcaagctt
    14761 tgaacagcat gcacaaaaag cattgggtat gatgatgcaa gaagtgatta aggcatctcg
    14821 ggaacagctt ggcgcagaaa aacgcgaaat tagcgaaacg attgccaaag atctccaaac
    14881 acaaaccaat acgtttaagg aagttgctca gtcgctgcgt atcgaaatcg acgcacggca
    14941 acgggagatt cgacaactcg aagaagatcg taataaaaaa tacggtcaaa tttcgcaagc
    15001 tctcaccgat tataaatctc tcactgagga gttgcgtggt tcgactgaac agcttaagcg
    15061 agttctctcg agcaatcagc aacggggaag ttggggggag atgcaagctg agaaaattct
    15121 tgaagcagca ggaatggtgg ctggtcagca ttacgccaag cagcagccga tcgaagggtt
    15181 tactgggctc cgcccagatt ttactctctt cttgccaaat aaaatggaac tctatgttga
    15241 tgtaaagttt cctttaaccg ccttgcaaga agtgtattcg agtaccacca aagaagatca
    15301 agagcgctac tttaaacgct tcggtgatga tttgcgagac cgtattaaag aagccggtaa
    15361 gtacgtgatt gataaatcaa actgcgttga ttacgttatc ctctttgttc cctcagagag
    15421 tgtgtttgaa attattaata aacatttccc agctatagtt gatgcttcat tcagtcagca
    15481 ggtcattctt gtgtctcccc actcgttctt tgcggttgtt cgtacgattc aagagtcata
    15541 tactcatttt tattatgaac aaaacctaaa agaaatcctt ggctacttac aacaaacgct
    15601 ccagcaattc gagcgtttca aaggcgagtt tgcggatgta ggca
//
LOCUS       DASWYB010000030         5235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_634922, whole genome shotgun sequence.
ACCESSION   DASWYB010000030 DASWYB010000000
VERSION     DASWYB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634922"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VGE59_01720"
     CDS             complement(<1..742)
                     /locus_tag="VGE59_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074399.1"
                     /translation="MLVYWNIVQFNFQRFLVYPLEIGAAFLRRAIDITFLVLFWNVVS
                     QSSGGSISAVPLVAYFLIATSVDVFTQSARLTFAKYINYAVKNGTISNYFIRPLPEIP
                     HLYASFLGSEGLSFCIAFVTLVVGIILAHVSSILTFLAFIGFLFVAIILSFAINLLVG
                     LLSFYTPEATGLRFTVGHVIRVFSGLIIPLSFFPDTLKAIFLFTPFPSMVFGPTSVLA
                     GQSSSFETWMSFVVAIIWIIILLPLSLWL"
     gene            complement(797..1168)
                     /locus_tag="VGE59_01725"
     CDS             complement(797..1168)
                     /locus_tag="VGE59_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1074400.1"
                     /translation="MPTILLVDDDTEMLRLYKMILEEKFDVLTAESGEKALTVVKESH
                     PDLVLLDIMMPAMNGIEVLEHIKADPETSATAVAMLTNLADENYKKSALEKGAKKYLI
                     KSEFEPDELMKEIASLIPSAA"
     gene            complement(1179..3287)
                     /locus_tag="VGE59_01730"
     CDS             complement(1179..3287)
                     /locus_tag="VGE59_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1074401.1"
                     /translation="MAEPNQDISYQLATERRQMGFLYVICGLSIFGLTVYLLIILFVN
                     HSFNDLRYVTPLLAFYIWLLTQVRKRKISQSAQIVALVGGVGYFLQLYTDVHDISTLF
                     FFNIYPIFVFFLLGKRGGVGYTGIFASGLVIAYALHLAGIVPLYFEPSDMRDFAIAFL
                     IASVMVYYYQDLIAASQEIIRERDMLLAKERDQARAVITSIGEGVFVIDKNGLTRSIN
                     PVTQSLLGIPSAEAIGQPIRALFTLSKNDIPLQEEEYPGIRALHTAQTIAVSIDDDIY
                     CHTANTTFPISIVASPIRTATEAIDGAVIVFRDITADKKAKELIEQEVQERTKELEQT
                     RVRLLASINSLDHGYIMTNHSPEVVLANNAAYAILRHTRAEEKTDIGISSPGENLTLS
                     QMQQKFGSAYDLSQKIQSVMETKTLARMKDVPYGDRILEVVISPIILRSDVIGTVILM
                     QDVTELKVIERSKDEFFSIASHELRTPLTAIRGYSALLQRKVASDNADKDIRSMVEGI
                     HNESARLIAIVNDFLDSSRLEQKRMVYSKEELDILDIINDVKHDFDLAAGEKDIALTV
                     IAPPHLPAVIADRQRTKQILVNLVGNAFKFTQSGAITIQPYVSDGYVGVAVHDTGPGI
                     PISNQPLLFHKFQQAGSSIITRDVSHGTGLGLYISKLLAQGMGGTVWLVKSEVNKGST
                     FAFVLPQAGGKALGKKHETV"
     gene            3431..4042
                     /locus_tag="VGE59_01735"
     CDS             3431..4042
                     /locus_tag="VGE59_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HEY1074402.1"
                     /translation="MKHRSLFSDPRFYFCLLILVIGASANFYSSALIQQLFPYRLPIQ
                     DTLFQILPYWPWTQNWSDWANIFSILLLIVYLFPKRSRLVPFVMLSFGVGYFLRSILI
                     LLNPFGGPLGNVTHYGLTTVEQHGQFPSGHIFLVTLVYFIIHRDERMLKQLALVSIFI
                     EIAALLLSHGHYSIDIVGGLFLGYFVAHSLQPYETSLSIKSAN"
     gene            complement(4039..4344)
                     /locus_tag="VGE59_01740"
     CDS             complement(4039..4344)
                     /locus_tag="VGE59_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074403.1"
                     /translation="MNKIDTLTTLSPLAALLREYKFPEEDIVRYMSQLNRQIEIGAIW
                     SLAKSRGDDLAHLAHFTPEQMKEYLASFSQDEYYAAMNVSAQEYTTDFLESIERGIA"
     gene            complement(4341..4901)
                     /locus_tag="VGE59_01745"
     CDS             complement(4341..4901)
                     /locus_tag="VGE59_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074404.1"
                     /translation="MHREDQEFSPESEQEAQSFHPEQQIRVDAVEGTTEREFPLELSE
                     SIRGQLEERLNDYAEQQEELTGGRVAEWTAVGKIWLQNAFIETMTRLAAAEPNAVVRA
                     FGSGDPLTILSESADDLVELVGPLIVSRVVEVYRDDITAFQMKRKEFLSEVTRVKERI
                     TAAIERRTTPLPPAAPTQEWATTGAS"
     gene            complement(4974..>5235)
                     /locus_tag="VGE59_01750"
     CDS             complement(4974..>5235)
                     /locus_tag="VGE59_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074405.1"
                     /translation="INPDKIDQELVTDFFDFAIDVDLPKRIEKITIITSDNDDADILK
                     SVELLKAAWPTCHVVSLSGMGHFTLSDMGTAEFPQLLDTILA"
BASE COUNT         1545 a         1244 c         1042 g         1404 t
ORIGIN      
        1 acagccacag cgaaagaggt aagagaataa ttatccagat aatagcgaca acaaaactca
       61 tccatgtctc aaaagacgag gactggccag caagaacaga agttgggccg aaaaccatgc
      121 tggggaaagg agtaaaaaga aaaattgctt ttaaagtatc cggaaaaaaa gaaagcggga
      181 taataagacc ggaaaacaca cgaataacat gtcccactgt gaaccggagt cccgtcgctt
      241 caggagtgta aaaagaaaga agaccaacaa gaagatttat cgcaaaagaa agaataattg
      301 caacaaacaa gaagccaata aacgccagaa aagtcagaat actagagaca tgagctaaaa
      361 taattccgac aactagcgtt acaaaagcaa tacaaaaaga gagcccttca ctgccgagaa
      421 atgaggcata taaatggggg atctctggca acggacgaat aaaataatta ctaatcgttc
      481 catttttaac agcatagtta atatatttgg caaaagttaa acgggcagat tgggtaaaca
      541 catccacaga agtcgcaatc agaaaataag ccactagagg cacggccgat atactaccgc
      601 ccgagctctg cgatacaaca ttccagaaga gaactaaaaa agtaatatca atcgcacgac
      661 gaagaaaagc ggcacctatc tccaaaggat aaaccagaaa tcgctgaaag ttgaattgaa
      721 ctatattcca gtaaacaagc atataaatac tataacacct cccaactaaa aaacacccaa
      781 tgggtgtttt ttagtcctag gctgcgctcg gaatcagcga agctatttct ttcatcaatt
      841 cgtcgggttc aaattcgctt ttaatcaagt attttttggc ccctttttcg agagcggact
      901 ttttataatt ctcatcagca agatttgtca gcatagccac ggccgtcgca gaggtttcgg
      961 ggtccgcctt aatgtgttct aatacctcga tcccattcat cgcaggcatc atgatgtcca
     1021 gcaggactaa atcaggatgc gattctttta cgactgttaa ggccttttca ccgctctctg
     1081 cagtcaatac atcaaacttc tcctcgagaa tcattttata gagacggagc atctcggtat
     1141 catcgtcaac aagtaaaata gtaggcattt ggttcatact atactgtctc atgctttttg
     1201 ccaagtgcct ttccaccagc ctgcggcaaa acaaaagcaa aagttgaccc tttattaacc
     1261 tctgacttca caagccaaac cgttcctccc atcccctggg caaggagctt tgagatatag
     1321 agccccaatc ctgtgccatg cgaaacgtca cgagtaataa tacttgaacc agcttgttga
     1381 aacttgtgga ataaaagcgg ttgattcgaa ataggtatcc caggccccgt atcgtgcaca
     1441 gctacaccaa catacccgtc agaaacatat ggttggatcg taatcgcacc cgactgagta
     1501 aacttaaagg cattacctac cagattgacg agaatttgct ttgtgcgttg tcggtcagcg
     1561 ataacagccg gtaagtgcgg tggagctata acagtgagcg caatatcctt ctctcctgcg
     1621 gctaagtcaa aatcatgttt aacatcattt ataatatcga gaatatctaa ttcctctttg
     1681 ctatagacca tccgtttctg ttcgaggcgt gaagaatcta aaaagtcatt tacaatcgca
     1741 attagacggg cgctttcgtt atgaattccc tccaccatgg aacgaatatc tttgtcggca
     1801 ttgtccgatg ccaccttacg ctgcaaaaga gcgctatacc ctcgaatagc cgttagtggt
     1861 gtgcgcaact catgtgaggc gattgagaag aattcatctt tcgaccgttc aattactttc
     1921 aattccgtaa catcctgcat aaggataacc gtaccaatga catcgctgcg caaaataatg
     1981 ggtgagataa caacctctaa gattcgatcc ccatatggca cgtccttcat tcgtgcgagc
     2041 gtctttgttt ccatcactga ctgaattttc tgagaaaggt catatgctga accaaatttt
     2101 tgttgcattt gtgacaacgt caagttttca ccgggggaac taattcctat gtccgttttt
     2161 tcctcagccc gggtatgacg caaaatagca taggcagcat tattggcgag tacaacttcc
     2221 ggcgaatggt tcgtcataat atacccatgg tccaagctgt taatggacgc caaaaggcga
     2281 acacgggttt gctctaactc cttggtacgc tcctgcactt cttgctcgat caattctttc
     2341 gcttttttat ctgccgttat gtcacggaaa acaataaccg caccatcgat agcctccgtg
     2401 gcagtacgaa tgggagaagc gacaatcgat atgggaaatg tcgtgttggc cgtatggcaa
     2461 taaatatcat catcaatact gactgcgatt gtttgtgccg tatgaagggc ccggataccg
     2521 gggtactctt cttcctgcag gggaatatcg ttttttgata gcgtaaagag cgctcggatc
     2581 ggctgaccaa ttgcttcagc cgagggaata cctaaaaggg attgtgttac tggattgatc
     2641 gaccgggtta agccattttt atcaataacg aaaacaccct ctccaatgga agtaataacc
     2701 gctcgcgcct gatctctttc tttagcaaga agcatatctc gttcgcggat aatctcttgg
     2761 ctggcggcaa taaggtcttg gtagtagtaa accatgaccg aagcgatcaa aaatgcaatc
     2821 gcaaaatcac gcatatctga aggctcaaag tacaaaggca caataccggc aagatggaga
     2881 gcatatgcaa tgactaatcc agaagcaaaa ataccggtat acccaacccc tcctctcttt
     2941 ccgagtaaga agaaaacaaa aattggatag atattaaaga aaaagagcgt tgaaatgtcg
     3001 tgaacgtcag tatagagctg caagaaatag ccgacccccc caaccagcgc cacgatttgc
     3061 gctgactgtg aaatttttcg tttacggact tgcgtcagta accaaatata aaaggccaac
     3121 agaggcgtaa cataacgtaa gtcattaaat gaatgattta caaacagaat aatcaacaaa
     3181 taaacagtaa ggccaaaaat acttagccca cagataacat acagaaaacc catttggcgg
     3241 cgttctgtcg caagttgata actaatatct tgattgggct ctgccacctt tttattatgc
     3301 gctaaagcac tcactctgca aagccgacgc gccactatct aaacgcatcc cccttggacc
     3361 atgttcattc ttttttaatc cctgtataat tctaggcagt gacgaattca tcctattcaa
     3421 aagtacacct atgaaacacc gttctctttt ctctgatcct cgattttact tctgcctgct
     3481 tattctagtg attggcgcga gtgccaattt ttatagcagc gcccttattc agcagctctt
     3541 cccataccgc ctaccaatcc aagataccct ttttcaaatt ttgccctact ggccttggac
     3601 tcaaaactgg agcgattggg caaacatctt tagtattctc ttgctcatcg tctacctttt
     3661 cccaaaacga tccaggcttg tgccatttgt gatgctctca tttggagttg gctattttct
     3721 acgtagcatc cttattttgc tcaacccgtt tggtggcccg ctaggaaatg tcactcacta
     3781 cggacttacc actgttgaac aacacgggca gttcccctca ggccatattt ttcttgttac
     3841 gcttgtttat tttattattc accgtgacga acgtatgcta aaacaactcg cactcgtcag
     3901 tatttttatc gaaatcgccg cgctccttct ttcccacggc cattactcta tcgatatcgt
     3961 gggcggcctt ttccttggat attttgtggc ccactcactt caaccttacg aaacatccct
     4021 tagtataaaa tcggcgaact aggcaattcc ccgttcaatt gattctaaga agtcagtcgt
     4081 atattcttgc gccgacacat tcatcgcggc ataatattca tcttgggaga aagaagcgag
     4141 atactctttc atttgttctg gtgtgaagtg ggcaagatgc gcgagatcgt cacctctgga
     4201 tttagctaag ctccatatcg ctccaatctc tatctgacga tttaattgac tcatatagcg
     4261 cacaatatct tcttccggaa atttatattc ccgtaaaaga gcggcaagag gagacagcgt
     4321 agtaagggta tctattttat tcatgatgca ccggttgttg cccactcctg tgtaggagca
     4381 gcagggggaa ggggtgtagt acgacgttca atcgcggcgg ttatacgctc tttgacacgg
     4441 gttacctcgg atagaaactc tttacgcttc atctgaaaag ccgtgatatc atcccgatac
     4501 acttctacca ctctcgagac aatcaaagga ccaaccaatt caactaggtc atccgctgat
     4561 tcggaaagaa ttgtcagagg atccccgctg ccaaaggctc gaaccaccgc atttggctca
     4621 gctgccgcta atctcgtcat agtttcgatg aacgcatttt gaagccatat cttcccgact
     4681 gcagtccact cagctacccg accacctgtt aattcttctt gttgttccgc atagtcattg
     4741 agcctttctt caagttgtcc acgtatactc tctgacagct ctaaaggaaa ctctctctca
     4801 gtcgtacctt caacagcatc cacgcgaatc tgctgttcag ggtgaaaaga ttgtgcttcc
     4861 tgctctgatt caggggaaaa ttcttggtct tctctatgca ttctcactca ctatttatag
     4921 tacgttacta ataagtatta cgtgttcacg ggcgcaatac aagtaaataa cttctacgcc
     4981 aagatcgtat cgagcaactg aggaaactca gcggtaccca tatctgaaag agtaaagtgc
     5041 cccataccag acaaactaac aacgtgacac gttggccaag cagccttgag taactccacg
     5101 cttttgagaa tatccgcatc atcattatca gaagtaataa ttgttatttt ttctatccgt
     5161 tttggaagat cgacatctat tgcgaagtca aaaaaatccg tcactaattc ttggtctatt
     5221 ttatcggggt taatc
//
LOCUS       DASWYB010000031         8783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_180556, whole genome shotgun sequence.
ACCESSION   DASWYB010000031 DASWYB010000000
VERSION     DASWYB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180556"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGE59_01755"
     CDS             <1..214
                     /locus_tag="VGE59_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074406.1"
                     /translation="DPMDGRYITSMYDLATGTTITFDPISPNDSYEQGCWLGTKRGKN
                     NLEYNLCKSSSGEWIVLEGPTKSYER"
     gene            complement(282..671)
                     /locus_tag="VGE59_01760"
     CDS             complement(282..671)
                     /locus_tag="VGE59_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY1074407.1"
                     /translation="MHTDIATYNHKLSGEPKEICEMLAQHIDANLEGAESKVWHGAPV
                     WFLEENPVAGYAVLKDAVQLLFWSGQSFDEDGLEAEGAFKAAQKRYTSKDEINTTDLK
                     RWLHKAADIQWDYKNIVKRKGELIRLK"
     gene            complement(682..1107)
                     /locus_tag="VGE59_01765"
     CDS             complement(682..1107)
                     /locus_tag="VGE59_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY1074408.1"
                     /translation="MAAPKTKPTTASVRDFLNKIEPEQKREDSFVLLELFEKVTGKSG
                     VMWGESIIGFDTYHYKSERSSQEGDWLMTGFSPRKQNLTLYIMAGNRENPALAKLGRH
                     KTSGGIGGCLYINKLADVDMKVLAELIKTSYEYMRKQYK"
     gene            1167..1535
                     /locus_tag="VGE59_01770"
     CDS             1167..1535
                     /locus_tag="VGE59_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEY1074409.1"
                     /translation="MKTDEKSLARVYAYTFASIYSLYITKAERKGRTKDEVDEIIRWL
                     TGYSPKELEAQIEKQATLEDFFSDAPAMNPLRSLIKGMICGVRVEEIEEPIMREIRYL
                     DKLIDELAQGKAMEKILRKQ"
     gene            complement(1597..3012)
                     /locus_tag="VGE59_01775"
     CDS             complement(1597..3012)
                     /locus_tag="VGE59_01775"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY1074410.1"
                     /translation="MQTSFKIGIGVIIAAAIGGTAWVLTKKQEPQPTSTSVAMTETEG
                     TGDKVVDRLIAGPDVITSLTFDCTPRNEHERYRTDRTLVVDPTDPNIMYINVEWKGVY
                     KTTDGGQTWAQKVKGIKVYANKDDTTKGCYSEYPVIKMDPNDHLHLVLGASGGGGGNL
                     SLKEPNSQTGGAYQSFDGGETWKMMINSKMNFYLNDVAFDSKNPATIYYGTSSNPASW
                     QGSDQNKIYVKDGLVYKTTNNGKTWTELPTGIGQNSAATFVMVNPKNPEELVVPTYSA
                     KRLSADGTGTGVSTGKDFSVAQMGILHSLDGGNTWSKLTDIPGNPSLVYGAFASTDFT
                     HMFFRVDGQTADTIAGYVTSDGGTTWKKTKYLDLVSYDPHDPTAMHMVGYTTVVVGPG
                     SQALHMWESKDGGVTWSKLGTLPKEITDATNTPYRPTQIEWHPTDKNTFYMTGGGGHI
                     WKTTDLARTWSTLLSVEKLPK"
     gene            complement(3092..4369)
                     /locus_tag="VGE59_01780"
     CDS             complement(3092..4369)
                     /locus_tag="VGE59_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074411.1"
                     /translation="MSNAVILEPLIEGTVVIKSRVRFFLNKEFSKKYTREALTLTYPS
                     EIDLESLPPSVLNIPLITNVIATVWFSGNRYTIEEMDEDLYYSLQKIKKFFQRFFYNT
                     EWEGELIPGRLVKNSIPQTHLQSASMFSGGLDSTATVLRHINENPMLISLNGLHKQAL
                     DFVAVHQLAFNQINTNSKDFLKLSYLNKASIDITKWFWDTSMGLSLVGTAAPFLYAKG
                     IPVLYIPAGFTWRHFLFPDGQTLQQPASPLIDENLSPMGLRVVHDGFSMTRTDKIKFI
                     STFCSQHSIPKPQLVVCTYCRTSDNEYTHCHKCFKCQITMLDIMAIGDRLHEYGFTQP
                     ESEFIFGFKYYVEKVTMRRGGTYGALNDTQQYVKHNLEKLPRAYHSFFEWFASIDFES
                     RLVTSSSRPLRSKSFKWSDYEDVYPGVKDYVDE"
     gene            complement(4390..5211)
                     /locus_tag="VGE59_01785"
     CDS             complement(4390..5211)
                     /locus_tag="VGE59_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEY1074412.1"
                     /translation="MADNNTTFSKKILRRGIFAPALIAVISLFLISQLVKAEVNKVAD
                     NTKIGPAGPTGATGEPGPKGEMGNPGPEGEVGPTGVPGSPGKAGATGTTGAMGATGAT
                     GEAGAAGATGATGSTGAPGTYSPSYGQLSLSSQDFDMTVSNEWLPIPFATAGPSSNMT
                     LSTTSPAKMTVQKTGVYQVNAAVNFLGEAADEGWFMATDYKLGIKINGGATTTVATLH
                     ASDSGRYSFNYSNIMQLSAGDYVEFYMTATDTHTYPNYNNLTLENSHAYLMQIAD"
     gene            complement(5298..5963)
                     /locus_tag="VGE59_01790"
     CDS             complement(5298..5963)
                     /locus_tag="VGE59_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY1074413.1"
                     /translation="MIKAVIFDIDDTFSLTEAVCFEVENEVLRRMGLPPMSREIHLAT
                     WEMRPSEAIPLRSAGVDVATFYALYENLLAEYIEADRLDVIPDENYKALDQLTDLGMA
                     LMVLTSRPHDRVQHMLQPEHRLARKIKDLRYRNNTKFRKPDPRAFDELLHGASLSPEH
                     CVYVGDAVRDATAAKKAGLYFIATLESGLRQRGDFDGVPVDMFIDKFPDVVSAVMRLE
                     SSF"
     gene            complement(6086..6883)
                     /locus_tag="VGE59_01795"
     CDS             complement(6086..6883)
                     /locus_tag="VGE59_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003733676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY1074414.1"
                     /translation="MKIYSWNVNGIRAVLRKDAFKPFIESEKPDILCIQETKAQPGEA
                     VIDLPEYKEYWNSAQKKGYSGTAIFSKVEPLNILHGLPDHIKEKYDMDSDGYGDPDTE
                     GRVLTAEFEKFYVVTVYTPNSKDDLSRIPLRKDHWDPAFLEYCQYLEKAKPVVFCGDL
                     NVAHTELDLANPKPNVGKRGFTYEERAGFQAFLDAGFVDTLRMFKEGNGYYTWWSPFG
                     GARDRNVGWRIDYFLVSAALRDKVITADIHPHVVGSDHCPVSLTLDV"
     gene            complement(6898..7596)
                     /locus_tag="VGE59_01800"
     CDS             complement(6898..7596)
                     /locus_tag="VGE59_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074415.1"
                     /translation="MNNVILGYSTAFYDPSTYGINQRIQEICEIQTNAIELVPNDDGR
                     FTELLTQESVELVRACHYRSIHAPALLEYSQDLVVSLLDLARDINAQVIVFHPDKTQD
                     FQKLHNQFGSLIAIENMDRSKTFGNTVRDLEEVFKKIPNAYWVADVNHLYSLDTSLRL
                     NREMHDAFSNRLHHYHVSGYGGLHSCLSLTHEVAILDAVQDLSKPLIHEGGIMVKHLL
                     KHEDEYVKNYLLRI"
     gene            complement(7671..8378)
                     /locus_tag="VGE59_01805"
     CDS             complement(7671..8378)
                     /locus_tag="VGE59_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY1074416.1"
                     /translation="MLKVASAQCSIIQNNILKAIAHINKGIAWAEQYTVDVLCFPECY
                     LQGYFLDRDQAKQVALDCAGEKFREVLSQIKGTPVTLIVGLIEIEGGLLFNTAVVIEN
                     GQLIGKYRKAHPNEKIYTAGNDLPVFWKKGVKYGINICNDANYPDTAKKLKDQGAEII
                     FFPLNNRLPNNTADLWRGKSKENLINCAKNNNCWVVSADVVYKGKDEKSFGCSLIVDP
                     AGEIQVMAKEFKVQLIK"
     gene            complement(8394..>8783)
                     /locus_tag="VGE59_01810"
     CDS             complement(8394..>8783)
                     /locus_tag="VGE59_01810"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY1074417.1"
                     /translation="FWYSAGMIDNKPTTVDAYIQSCAENVRTMLNEVRSIILQALPDA
                     SESLKWGQPAYSYETIMVSFAAHKNHMNFYTTPSTIRALESDLARYKIGKGSIQFPYN
                     IQLPKTLIKKVIEYRLKEYKEQGIKWM"
BASE COUNT         2444 a         2137 c         1707 g         2495 t
ORIGIN      
        1 cgatcctatg gacggccggt atatcacctc aatgtacgac ctggccaccg gtaccactat
       61 cacctttgac cccatttctc ccaacgacag ttacgaacaa ggctgttggc tgggtacaaa
      121 acgcggcaag aataacctag agtataacct gtgcaaatca tcatcaggtg agtggatagt
      181 tctcgaaggc cccacaaaat cgtacgaaag ataatccgat ctctcctcac caaaaaaaac
      241 agctgaagga gctgtttttt ttggtgagga gaataacgat gctatttcaa acgaatcaat
      301 tcacccttgc gtttgacgat atttttgtag tcccactgaa tatcagccgc cttgtgaagc
      361 caacgcttga gatcggtggt gttaatttcg tctttactcg tgtaacgttt ttgagccgcc
      421 ttgaatgccc cctcggcctc aagcccgtct tcgtcaaacg attgcccact ccagaagagc
      481 agctgtactg catcttttag aacagcgtag cccgcaacag gattttcttc taaaaaccag
      541 accggtgccc catgccaaac tttgctttct gcgccttcaa ggtttgcatc aatatgctgg
      601 gccagcattt cgcaaatttc ttttggttct cccgataatt tatggttgta agtagcgata
      661 tctgtgtgca ttatgattcc tctatttata ctgtttcctc atatactcat acgacgtctt
      721 aataagctca gcgagaacct tcatatctac gtcagccaat ttattaatgt acaagcaccc
      781 cccaattccc ccacttgttt tatgccttcc aagttttgcc agggctggat tttctctatt
      841 tccagccata atataaagtg taagattttg tttacgcggc gagaaccctg tcatcaacca
      901 atctccttct tggctgcttc gctcagattt atagtggtac gtatcaaatc caataatgct
      961 ttcaccccac atcacacctg acttacccgt tactttctca aacagctcaa gcaatacaaa
     1021 gctatcttcg cgtttttgct cgggttctat tttgtttaaa aaatctctca cacttgcagt
     1081 agttggtttt gttttaggtg ctgccatact agtaacacta taccaaaaaa gtgctcccat
     1141 agccctattt gtgctagaat aacactatga aaactgatga aaaatctctc gctcgagttt
     1201 acgcgtacac gttcgccagc atctactcac tttatattac aaaagccgaa agaaaagggc
     1261 gcacgaaaga tgaagttgac gaaattattc ggtggctaac cggctacagt ccaaaagagc
     1321 tcgaagctca aattgaaaaa caagcaactt tagaagactt tttctccgac gcacccgcta
     1381 tgaacccctt acgttcgtta atcaaaggca tgatctgtgg cgtacgcgtc gaagaaatcg
     1441 aagaaccaat catgcgcgaa atccgctacc tcgacaagct catcgacgaa ctcgcacaag
     1501 gaaaagcgat ggaaaagatc ctacggaagc agtagcactc cacccgtaaa aaagagactg
     1561 gcacatacac atgccagtct cttttttctt tgggctttac tttggtaact tctcaaccga
     1621 aagtagggtg ctccaggttc tggcaagatc ggttgtcttc caaatatgcc cacccccgcc
     1681 ggtcatgtag aacgtgtttt tgtcggtagg gtgccactca atctgtgtcg gccgatacgg
     1741 cgtgtttgtg gcatcagtaa tttcttttgg aagagtacca agcttgctcc aggtaacccc
     1801 accgtcttta ctttcccaca tatgaagggc ttgtgaccct ggccccacca ctaccgtcgt
     1861 gtaacccacc atatgcatcg ccgttgggtc gtgcggatca tagcttacaa gatctaagta
     1921 cttcgtcttc ttccaggtcg taccgccgtc cgaagtaaca taacctgcaa ttgtgtccgc
     1981 cgtttgacca tcaacgcgga aaaacatatg cgtaaagtca gtagacgcaa aggctccgta
     2041 cacaagactt gggttccccg gtatgtcggt caatttcgac caggtgtttc caccatcaag
     2101 actatgtagg atacccattt gcgcaacact aaagtctttg cctgtactca ctcctgtccc
     2161 tgttccgtca gccgagagcc gttttgccga atacgtgggg accactaatt cttccggatt
     2221 ctttggattt accatcacaa aagtggccgc actattttga ccaattcctg tcggcagctc
     2281 agtccacgtc ttaccgttat tggtggtttt gtacaccagg ccgtctttga catagatctt
     2341 attctggtcc gacccttgcc acgaggcagg gttagatgat gtgccataat aaatcgtcgc
     2401 gggatttttt gagtcaaagg caacatcgtt taagtaaaag ttcatcttgc tgttaatcat
     2461 cattttccag gtttctccgc catcaaacga ttgataagcg ccacctgttt gggagtttgg
     2521 ctctttcaga ctcaagtttc ctcccccacc accactcgcg cctaacacaa ggtgaaggtg
     2581 atcattcgga tccattttga tgactgggta ctcgctgtaa cagcctttgg ttgtatcgtc
     2641 tttattggca tacactttaa tacccttaac tttttgggcc catgtctgcc cgccatcagt
     2701 tgttttatac acgcccttcc attcaacgtt gatatacata atattaggat ctgtcggatc
     2761 gacaacaagc gtacggtcag tgcgatacct ttcatgctca ttacgtgggg tacagtcgaa
     2821 tgttagtgat gtaataacgt caggcccagc aataagtcta tcaacaacct tatcgccagt
     2881 tccttctgtt tctgtcatag ccacactggt acttgttggc tgtggctctt gcttctttgt
     2941 taatacccac gccgtgccgc caatcgcagc cgcaataata acccctatcc cgattttaaa
     3001 tgatgtttgc atagcttcag catatcaaaa aagcagccaa ctgcctcgta acccacgaaa
     3061 gattcaggtc tttttgcaac cgtcaacaat gctactcatc aacataatct tttaccccag
     3121 gatagacgtc ttcgtagtca ctccacttga acgattttga ccgaagagga cggctcgacg
     3181 aagtcacaag tcgagactca aagtcaattg aagcaaacca ttcgaaaaat gaatgatatg
     3241 cccgaggcag tttttcaaga ttatgcttta catactgttg cgtatcgttt aatgctccgt
     3301 aggtccctcc cctacgcata gttactttct cgacgtaata tttaaacccg aaaataaatt
     3361 ccgactcggg ttgagtaaag ccgtactcat gcaaacgatc accaatcgcc ataatatcga
     3421 gcatcgttat ctgacatttg aaacatttat gacaatgagt atactcattg tcagatgttc
     3481 ggcagtacgt acataccacc aactgaggtt tgggaatact gtgctgcgaa caaaatgttg
     3541 agataaattt gatcttgtcc gtacgcgtca tcgaaaaccc gtcatgaaca actcgtaatc
     3601 ccatgggcga taaattttcg tcaatgagtg gggaagctgg ctgttgcaat gtttgtccat
     3661 caggaaacaa aaaatggcgc caggtaaaac cggcaggaat gtataacact gggataccct
     3721 tggcatacaa gaaaggagcc gcggtaccaa ccaatgacag gcccatactc gtatcccaaa
     3781 accatttggt gatatcgatc gaggctttat tgagatagct gagcttaaga aaatccttag
     3841 aattcgtatt aatttgatta aacgccagtt gatgcacagc cacaaaatca agagcctgct
     3901 tgtgcagacc gttgagagaa ataagcatcg gattttcgtt gatgtgccga agcaccgttg
     3961 ccgtagaatc taatccaccc gaaaacattg aggcagactg taaatgcgtt tgaggaatgc
     4021 tatttttgac taaacgtccg ggtataagct caccttccca ttccgtatta taaaaaaaac
     4081 gttgaaagaa tttttttatt ttttgtaacg agtagtagag atcttcatcc atctcctcga
     4141 tcgtataccg attaccagaa aaccaaaccg tcgcgatgac attggtaata agcggtatat
     4201 taagaaccga cggtggaaga ctctccaaat ctatctccga aggataggta agggttaatg
     4261 cctcgcgtgt gtacttttta ctaaactctt tattaagaaa aaaacgaact ctgctcttaa
     4321 taactaccgt accctcaatc aagggttcaa gaatgactgc gtttgacatg agcgaaagtc
     4381 aacgaggcac tagtctgcaa tttgcattag gtacgcatga ctattttcaa gtgtaaggtt
     4441 gttgtaatta ggatatgtat gggtgtcggt ggccgtcata taaaattcaa cgtaatcgcc
     4501 ggccgacagt tgcatgatat tgctatagtt aaacgaataa cgacctgagt cagaagcatg
     4561 aagggttgcc accgtggtcg ttgccccacc attaatttta ataccaagct tataatcagt
     4621 tgccatgaac cacccctcat ccgcagcctc accaagaaaa ttcacagcgg cgtttacctg
     4681 atagactccg gttttctgaa ccgtcatttt ggctggagac gttgtagaga gagtcatatt
     4741 cgacgaaggg ccggcagttg caaaaggaat aggaagccat tcgtttgaga cagtcatgtc
     4801 aaagtcttgc gaagaaagtg agagctgacc ataagagggc gagtacgttc ctggcgcacc
     4861 tgtggatcct gttgcacccg tggcaccggc tgcaccggcc tctcccgtcg caccagttgc
     4921 acccatggct ccagtcgtac cagttgcacc tgctttcccg ggcgatcctg gtacgccagt
     4981 cgggccgact tctccctctg gaccgggatt acccatttca cctttaggtc caggctctcc
     5041 cgtcgcaccg gtcgggccgg cagggccaat ctttgtattg tctgctactt tattaacctc
     5101 agccttcact aattgcgaaa ttaaaaaaag tgaaatcact gcaatcaaag cgggggcaaa
     5161 aatccctcga cgaagtatct tcttactaaa tgtggtattg ttatctgcca taaagtgcct
     5221 ttatgtttat ttttttcaca ccttttaccc atcatagagg agatcacaat acttgtaaaa
     5281 ctgacctccc ttcgtcttca gaaagagctt tctaaacgca ttacggcaga gacaacatcc
     5341 ggaaatttat ctataaacat atcaaccgga actccgtcaa aatcccctct ctgtcttaat
     5401 ccgctttcga gcgtagcaat gaaatagagt cccgcttttt ttgccgcagt tgcatcacga
     5461 acggcatctc ccacatagac gcaatgttca ggcgacaaac tcgccccgtg taataactca
     5521 tcaaaagcgc gtgggtctgg cttacgaaat tttgtattat tccgataacg caagtccttt
     5581 atctttctgg ccagcctgtg ttctggttgt aacatgtgct gaacacgatc atgcggtcga
     5641 gacgttaaca ccatcaaggc cataccaaga tccgtgagct ggtcgagcgc tttataattt
     5701 tcgtcgggga ttacatctag tcgatccgcc tcaatgtatt cggctaataa gttttcatag
     5761 agggcataaa aagtggccac atccacaccc gccgaacgga gtggaattgc ttcagatggc
     5821 cgcatctccc acgtcgctaa atgaatctcc cgggacattg gcggcagtcc catccgtcgc
     5881 aacacttcgt tttccacttc aaaacagact gcttccgtta ggctaaaagt atcatcaata
     5941 tcaaatatca ctgccttaat catttgttca gtgtaccaaa aaaacaacca taaagccgct
     6001 tcccagagaa caaaagagaa tggtgaaaaa tacgaaagtg agttaacaag caggttgtta
     6061 tcaccgctcg aaaaagatca ttgtcttaga cgtctagagt tagactaaca gggcaatgat
     6121 cagatccgac tacatgcggg tgaatatctg ccgtgatcac tttatctcgt agggctgcgg
     6181 aaactaagaa ataatcaatc cgccatccca cgttccgatc tcgagctcca ccgaatgggc
     6241 tccaccaggt gtaataacca ttcccttctt taaacatccg aagcgtatca acaaagccag
     6301 cgtctaaaaa tgcctgaaaa ccagcgcgct cttcataagt aaaacccctc ttgcccacat
     6361 ttggcttggg attcgccagg tcaagttcgg tatgagctac attaagatcg ccacaaaaca
     6421 caaccggctt cgccttctca aggtattggc agtactctaa aaaggctgga tcccaatgat
     6481 ctttccgaag cgggatccga cttaaatcgt ccttagaatt tggtgtgtaa actgtcacaa
     6541 cgtagaactt ctcgaattca gcggtcaata ctcgtccctc ggtatccggg tcaccataac
     6601 catcactgtc catgtcgtat ttttctttaa tgtggtcagg caatccatgc aaaatattca
     6661 acggctcaac cttactaaaa atcgccgtcc ccgaataacc tttcttctgg gccgagttcc
     6721 aatattcttt atattctggc aaatcaatga ctgcttcgcc tggctgagcc tttgtctcct
     6781 gaatacaaag tatatcgggt ttctctgatt caataaacgg cttaaaggca tctttgcgca
     6841 aaacagcccg aataccatta acattccatg agtatatttt catttccata attcatatta
     6901 tatgcgtaaa agataattct ttacatactc gtcttcatgt ttaagtagat gctttaccat
     6961 aattccccct tcatgaatca gtggctttga aagatcctgt acggcatcaa ggatagcaac
     7021 ctcatgcgta agcgaaagac aagaatgcaa tcctccatat ccacttacat ggtaatggtg
     7081 taatcggttg gaaaaagcgt cgtgcatttc tcgattaagt cgaagcgaag tatcaagcga
     7141 gtacaaatga tttacatctg caacccaata tgcgtttggt atttttttaa atacctcttc
     7201 aagatcacgc accgtatttc caaatgtttt acttctatcc atgttttcaa tcgcaatgag
     7261 agaaccaaac tgattgtgca acttttgaaa atcttgagtt ttatctggat gaaatactat
     7321 aacctgcgca tttatatctc gtgctaaatc tagtaagctt actactaaat cctgagaata
     7381 ttctagaagc gcaggagcat gtatagatcg ataatggcac gccctaacca actcaacact
     7441 ttcctgagtc agcaattctg taaacctacc atcatcattt ggcactaact caatcgcatt
     7501 tgtttgaatc tcacatatct cttggatgcg ttgatttatt ccataggtcg acgggtcgta
     7561 aaacgctgtt gagtatccta gtattacatt attcatacaa aaactatacc agaaaaacag
     7621 ctatcacgcg gctctccaca caagtagatt tctatcaaag acgttagaac ctatttaatc
     7681 agctgcactt taaactcttt tgccattact tgaatttcgc cagcaggatc aacaatcaac
     7741 gagcacccaa agcttttctc gtctttcccc ttataaacca catcagcaga aacaacccaa
     7801 cagttattat tttttgcaca attaatcaag ttttctttac tcttgcctct ccataggtct
     7861 gcagtattgt taggcaaacg gttgttaagc ggaaagaaaa taatttcagc accttgatct
     7921 tttaactttt tagcagtatc aggataatta gcatcgttac aaatattaat cccgtattta
     7981 accccttttt tccaaaaaac tgggagatcg ttgcctgcgg tgtaaatctt ttcgttcggg
     8041 tgagcttttc gatatttccc gattagttgg ccgttttcaa tgacgaccgc agtattaaag
     8101 agaaggccac cttcaatttc aatcagacct actattaaag ttactggtgt acctttaatt
     8161 tgactaagca cctctcgaaa cttttcacct gcacaatcta aggcaacttg cttcgcttgg
     8221 tctctgtcta aaaaatagcc ttgaagataa cattccggaa aacaaagtac atccacagta
     8281 tactgttctg cccatgcgat ccctttgtta atatgggcaa tagccttgag aatgttattt
     8341 tggataatgg agcattgcgc cgaagctacc tttaacatac gaagattata aacttacatc
     8401 cactttattc cttgctcttt gtattcttta agacgatatt caatcacttt ttttattaaa
     8461 gtcttaggca attggatatt gtaaggaaac tgaatcgatc cttttccaat cttataccgg
     8521 gcaaggtcgc tctcgagcgc tcgtattgta cttggggtag tatagaaatt catgtgattt
     8581 ttatgagccg caaaagatac catgattgtt tcataactat acgcaggctg cccccacttt
     8641 aaggactcag aagcatcagg taaggcctgt agaattattg accttacttc atttagcata
     8701 gtccgcacat tctctgcgca actttgaata taggcatcaa cggttgtagg tttattatct
     8761 atcatacccg cactatacca aaa
//
LOCUS       DASWYB010000032        11349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_18975, whole genome shotgun sequence.
ACCESSION   DASWYB010000032 DASWYB010000000
VERSION     DASWYB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18975"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            607..1662
                     /gene="dprA"
                     /locus_tag="VGE59_01815"
     CDS             607..1662
                     /gene="dprA"
                     /locus_tag="VGE59_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY1074418.1"
                     /translation="MEVILSALLSRYCHFYNVKLFELVKVYDTLESLYAIWLTNPVSL
                     PINLCRQLEVAWKETYKVEMQEQLHKHAIRGLLFNNQEYPDMLRHISDPPYVLYVRGN
                     SDVLSNVCLACVGTRRMSGYGKRAISRLLSALSHYSLCIVSGLALGVDGEAHRVALES
                     DLLTVAVLAAGLDRFEPTTNAPLAERIITQGGCLLSEYPPGVRPQKHHFLERNRIVAG
                     ISAATLIIEGKEHSGSLVTARYALANGREVGVVPGDIFLENTQGPLRLLRDGAWPIAE
                     VGDILNMLSLDVVSQPISDEVLGEVAMALRAGPVSLDGLCEKLNWPIGQVQAELTRLE
                     LEGAVECLATGAYYLKR"
     gene            1722..3980
                     /gene="topA"
                     /locus_tag="VGE59_01820"
     CDS             1722..3980
                     /gene="topA"
                     /locus_tag="VGE59_01820"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY1074419.1"
                     /translation="MAKKVVFVESPAKAKTIQKFVDKSTVVRATFGHIRDLPKSDLGV
                     DLEHDFAPKYVIPRKASPVVKELKALVKGSEEIYLATDPDREGEAIAWHVCEALGLDS
                     TKVSRILFHEITNEAIQHALEHPGKVNHNLVDAQQARRVLDRLVGYKLSPLLWKKIYR
                     GLSAGRVQSVALRLITDREDEIAAFEPKEYWSLWASFETKQKDHFLAKLEKVKGKSIG
                     KHPAQSVIEEAQAYGKDLQWVITDRITENKQRHPKPPFTTSTLQQEGSRKLHFSAKQT
                     MMHAQKLYEGISLGGETVGLITYMRTDSLNLAETAIADARTVVGQNFGPKYVPAEPRR
                     YKTKSKGAQEAHEAIRPTSFARTPDSVKQYLAPAEYKLYKLIWERAIASQMASADTEL
                     TQIHVGPEKEPTAVTYIASGVRIVFPGFLKAYEESRDEGAEVEVGDGEEQLLPLIVKG
                     DSVTLDSLEPKQHFTQPPARFTEASLVKEMERLGIGRPSTYAPTIATIIDRGYVAKEE
                     GKFVSQEVGKIVIELLKDKFPNIVDYDFTAKMENSLDNVADGEAKWQNVIKDFYGPFE
                     KSIELAAKDIGEKSLSEEATDEVCPDCGKPLVIKRGRFGRFYACTGFPDCRHTRPFDV
                     DKKAEAQLIEGRKCPNDGADLVLKSGRYGNFIGCSNYPNCKFIEAINKEMGIKCPKDG
                     GNIIERRSKRGKLFWGCDKYPNCDFVAWDRPMPQPCPKCKGIVVQKKKELACMSCDYR
                     KERDADEEVTEE"
     gene            3989..4606
                     /gene="recR"
                     /locus_tag="VGE59_01825"
     CDS             3989..4606
                     /gene="recR"
                     /locus_tag="VGE59_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY1074420.1"
                     /translation="MKQFLPRAIQDLSQALSRLPGIGPKTANRLAFYLINRPKEEVAE
                     IGAAFINLKENLLRCEVCHTVSETNPCPICADEERDHARIMVVEEPLDMVAMERTGYE
                     GIYHVLGGVISPINGVGPRDLTLQELFDRVQSGTIEEVILATDPSLEGEATASYITEK
                     LMQVAPKGLAISRIARGLPVGGDLEYADELTLRRALEGRQNYRTK"
     gene            4643..6001
                     /gene="cysS"
                     /locus_tag="VGE59_01830"
     CDS             4643..6001
                     /gene="cysS"
                     /locus_tag="VGE59_01830"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY1074421.1"
                     /translation="MSLSFLNTLTRTKETFVPITPGLASLYTCGPTVYDFAHIGNMRT
                     YIFNDTLRRVLEMNGLTVHHVMNITDVGHLVGDSDFAEDKVDAKAEKEGLHPLDIAKR
                     YETAFFEDFSRLNNKLPTDVVRATEAVDSQIELIKQLEARGFTYRDDDAIYFDTSKLS
                     DYGKLTGQTLSEKLIGAREEVVVDTRKRNPQDFVLWFFLTGRYAHHILHWASPWGEGF
                     PGWHVECSAISRSRLGQPFDIHTGGVDHIGTHHTNEIAQSEAAYGEPLAHYWLHGEHL
                     MVEGKRMGKSEGNLVRLTTVEEKGFDPLDFRYLVLTAHYRSRMNFTWDALVGARKTLT
                     SIRELVAHDTSEKGEYAQEELIRAAFNDDLDTPRALALLHQAKSGALWRKFDCVLGLN
                     VDHLPREKHEVNSEITALKEERDQARAQKDWAKADMFRHKIEALGYRVRDTESGSVLE
                     RL"
     gene            complement(6473..7096)
                     /locus_tag="VGE59_01835"
     CDS             complement(6473..7096)
                     /locus_tag="VGE59_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074422.1"
                     /translation="MQEAQCGHLLLDSEHLSQRVRSNDDINVVVILILIRGILDAEGV
                     SRHKFAGLNPTFQNAGEDVPLNKPVGAAFLLQVFLELGLVAFVLRLEADSAIVARTLA
                     LEELAPGEDFVLELLFPATAHFRVLTGLCIDVDSAIVGRDALTILSRRVHLVDDVEPH
                     PELCPTVDRDIYLAIGPEPPHHFGAGEQIGLSRRRYKQSSEQQDHQQ"
     gene            7199..7984
                     /locus_tag="VGE59_01840"
     CDS             7199..7984
                     /locus_tag="VGE59_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074423.1"
                     /translation="MQTPNNVGPDARFRRDGGAFDGTAYRPGQRNSFPSGGPAPSGSY
                     GDQTPHHVPPAPYTGAPLYGSGTMVPPSPPPASTPTEPPPGDPRPNVEIAPPAAPRAA
                     SGRGIDLDEEERFERGSSRRPESAPVPMGQSAGDNDLRPVGTKTGTLATLEFQFVLRS
                     GSFTEYSFKIGVVGYHPATGKPVDFTPGPNGQVLPKVFQVVGGKAQYAAQPGLIYVPY
                     FTGTVQKEWELPPDKRDGMFNEGRSPGWVVPEGGRRLVLTRRN"
     gene            7987..9186
                     /locus_tag="VGE59_01845"
     CDS             7987..9186
                     /locus_tag="VGE59_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074424.1"
                     /translation="MKWRHIIAALSLVVSALVALPAQAKKVYTEGQYGTSPLGPRKLD
                     MNNGQFVSGQAVDDYRRFMLSERVAVAAKEIGIAPHFWQVTKIAINDANQDYVVIPKG
                     TQRDLRVLIADSFAKGMWTETSKGSKIWYLRVQGFVPDRLDGEDMAFYSRVWNEVRGF
                     QEDFMFAPGFQPQCAGVLIYHEAAEQMVWVAMLEKCRNLWWASLHISIRILMEERLIP
                     GPRGPEGEQGPPGVRGPEGPIGPQGPPGWTGPRGPRGYPGPPGRDGEVRYVLGFQPNW
                     SFVPGGRQPTPAYVVDTKPGLIPTVAALMGFAQPFWARGNTYNIAGGRATGGNSVAIS
                     RQYQTSANFNEQNTDVTANGGAGGAGGNATNTTTVDNHPVNVNMNTNGNEVNGANSNG
                     GAAGAGK"
     gene            9356..10228
                     /locus_tag="VGE59_01850"
     CDS             9356..10228
                     /locus_tag="VGE59_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074425.1"
                     /translation="MQKLAILPVLVSAAAVALSGGVTPAQATNWEDYADAIAAQYQYQ
                     TSTNVNVQNQSTTVGATTPTTSTGGNATNVTNVTSQDTNINQNNNQNVVSIANVNGNG
                     SASASGSAAGTQTGTGTQTGTQTVNPTPTPTPTPTPTTTTGGNGTNVTNVTSQNGNIN
                     QNNNQNVIPIANVNGNGTATASGSASSTQNATITQTSNQSVNTGNQTGTPTPTPTSTT
                     TTSTSTSTSTSTSTGSKTTATASAFASTLEQLPAAGAAPALAGGALAGFGSIVAVIRA
                     WARRQKEILNDLIG"
     gene            10373..11344
                     /gene="prfB"
                     /locus_tag="VGE59_01855"
     CDS             10373..11344
                     /gene="prfB"
                     /locus_tag="VGE59_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY1074426.1"
                     /translation="MEKKQQLAELLGIENKKVRIAEIQKDMQSADFWADHVKAAEITQ
                     ELKQLDDIVTEFELAETPEEIAELEKYTLLSGEYDAEPAIMTISAGSGGTEAQDWAEM
                     LMRMYVRYSEKSGLTATILDISEGEEAGIKSATLEIKGPFAFGYLKAEAGVHRLVRMS
                     PFDADKARHTSFALVQVIPKLKPKTVSIDPKDLRVDVYRAGGKGGQGVNTTDSAVRLT
                     HLPTGLVVAVQNERSQLQNKEIAMAILLSRLELLQKEKNAEQVAFLKGDFREAAWGNQ
                     IRSYVLAPYQLIKDHRTGHESPSPQDVLNGDIQDFIEAGLRWMKEAP"
BASE COUNT         2939 a         2637 c         3064 g         2709 t
ORIGIN      
        1 tggtaaaagc gtaagaaagc cgagagaata aaaatgataa taacgcacca agtgaggaga
       61 gattgatgcg atcggttggc ttttgtttgt tccacctatt tattaaacca atggcagcag
      121 gaatttacaa tgccgtcgta ccgctggtag ctatctttag atatgaaaag agggtcagtg
      181 gttagactga ccctcttatt cccctccact acaccgcctt gcctccgttt ggattttggc
      241 accccgcgaa tatttaaggg ggcgggccaa ggtgtgtaat agagagggtt ctcggacgaa
      301 agataacgcc gaaggagggg cacagcgtat tttcgtgagt aaaactcacg tcttaagtcc
      361 gagaactctc tctactactc tagaggaatt atagaataga attttctagg tgtcaatgcc
      421 cttgtagtcg gtcgatatat aagctaatta ccgacctgac atatgtgact atagcacgag
      481 gatttaaaat tgtcaatacc tttacttgtg gccgtaacgc aagtagatgc aatggaatga
      541 aatattaaaa tattattata aagtatagag gcctggataa tactatgtta cgtttatttc
      601 tatatcatgg aagttattct ttcggcatta ctttctcgct attgtcattt ctataatgta
      661 aagctttttg aattagtaaa agtgtatgac acgttagaat ctctttatgc tatctggctc
      721 acgaaccctg tatctcttcc tataaacctt tgcaggcagt tggaggtggc ctggaaggaa
      781 acatataaag tggagatgca agagcaattg cataagcatg caattcgcgg ccttctgttt
      841 aataatcagg aatatccaga catgttacgg catattagtg acccgccgta cgttttatat
      901 gtgcgaggaa attccgatgt attgagtaat gtatgtttag cttgcgttgg aacgcgaagg
      961 atgagtggct atggaaaacg ggctattagc cgacttctct cagctctgtc gcattattct
     1021 ctttgtatcg tgtcgggttt ggcgttagga gtcgacggag aagctcatag agtggcactg
     1081 gagtctgact tgctaacggt tgcggttctt gcggcgggtt tggaccgatt tgagccaact
     1141 acgaacgcac cgcttgcgga gcgtattata acccagggtg gttgtcttct cagtgaatat
     1201 ccaccgggtg ttcgccctca gaaacatcac tttttagaac gaaaccgtat cgtagcaggg
     1261 atttctgcgg caactctcat cattgaagga aaagaacatt ctggttcgct ggttacggcg
     1321 cgctatgcgc tcgcaaatgg gagagaggtt ggcgtagtgc cgggggatat cttcctggag
     1381 aacacgcaag gtcctttgcg gttattgcgg gacggagcat ggccgattgc tgaagtgggt
     1441 gatattctga atatgctttc ccttgatgtg gtgtctcagc ctatatcaga tgaagtactg
     1501 ggggaagtcg cgatggcgct tcgggctggg ccagtatcgc tcgacggact gtgtgaaaaa
     1561 cttaactggc cgattggaca agttcaggcg gaattaaccc ggcttgaact cgaaggggcg
     1621 gttgaatgct tggctacggg cgcgtattat cttaagagat agttcatccc agtcatcgga
     1681 atgctagaca caatactcat atatttggta taaaagattg tatggcaaaa aaggttgtat
     1741 ttgttgagtc tcccgcgaaa gcgaagacta tccaaaagtt tgtagataag tcgacagtcg
     1801 tgcgagcgac gtttggccat attcgtgatc ttcctaaaag tgatttaggg gtagatcttg
     1861 agcatgattt tgctcccaag tatgtaattc ctcgaaaagc ctcgccggtt gtgaaagaac
     1921 tcaaggctct tgttaaagga agtgaagaaa tttatcttgc caccgaccct gaccgtgaag
     1981 gagaggcgat cgcatggcat gtttgtgaag cattaggact tgattcgacc aaagtaagtc
     2041 ggatcctttt tcatgaaatt acgaacgaag caattcagca tgcgctcgag catccaggaa
     2101 aggtcaatca caatttagtg gatgctcagc aagcacggcg cgtcttagac cggttagtcg
     2161 gttataagct aagcccactt ttatggaaga agatttatcg cggtctttcg gcaggacgtg
     2221 tgcagtctgt tgctcttcgt ttaattaccg accgcgaaga tgagatcgcg gcttttgagc
     2281 caaaagaata ttggtcgtta tgggcatctt ttgaaacaaa gcagaaagat cattttttag
     2341 ctaaactcga aaaagttaaa ggcaaatcaa ttggcaaaca ccccgcgcaa agcgtcattg
     2401 aagaagctca ggcgtatggt aaagatttac agtgggtaat cactgatcga attactgaaa
     2461 ataaacaacg ccatcccaag cctccgttta caacttcgac tctccagcaa gaggggagcc
     2521 gtaagcttca cttttcagct aagcaaacca tgatgcacgc gcagaagtta tatgaaggta
     2581 tctctctggg tggcgagaca gttggtttaa ttacctatat gcgtaccgac tcgctgaatt
     2641 tagccgaaac agcaatcgct gatgcacgta ctgttgtggg acagaatttt ggtccaaaat
     2701 acgtaccggc cgagccacgt cgctataaaa ctaaatcaaa aggggcacaa gaagcacacg
     2761 aagcgatccg gcctacaagt tttgctcgta cccccgattc cgtgaaacag tatttagcgc
     2821 cggcagaata caagctttat aaattaattt gggaacgagc tattgcaagt cagatggctt
     2881 cggctgatac tgagctgaca caaattcacg ttgggccaga aaaagaacct acagcggtta
     2941 cgtatattgc ttcaggtgtc cggattgtct ttcctggttt cttgaaagcg tacgaagaaa
     3001 gccgtgacga gggggctgaa gtggaagtgg gggacggcga agagcagtta ttgccgttga
     3061 ttgtaaaagg tgattctgtc acgcttgatt cgctcgaacc aaagcagcac tttacgcagc
     3121 cgcctgcccg ttttacagaa gcttctttag tcaaagagat ggaacgcctt ggtattggcc
     3181 gtccttcaac atatgcgcca acgatcgcaa cgattattga tcgtggatac gtggcgaaag
     3241 aagaaggaaa gtttgtatca caggaagttg gtaagattgt gattgagctt ttgaaagata
     3301 agtttccgaa tatagtcgac tatgacttta ctgccaagat ggaaaattct ctcgataacg
     3361 ttgctgacgg tgaagcgaag tggcagaacg ttattaaaga tttttacggt ccttttgaaa
     3421 aatcaattga actagcagcc aaagatattg gggaaaagag cttgtcagaa gaagctactg
     3481 acgaagtctg tcctgattgt ggtaagcccc ttgtgataaa acgaggacgg tttggacgat
     3541 tctacgcttg tactggcttt cctgactgtc gtcatacaag gccttttgat gttgataaga
     3601 aagcggaagc gcaattaata gaaggacgta agtgtcccaa tgatggtgct gatttagtgc
     3661 ttaaaagtgg tcgctacggt aactttattg gttgttccaa ctatcctaac tgtaaattta
     3721 tcgaagctat caataaagaa atgggcatta agtgtcccaa agatggtggg aatattattg
     3781 aacgtcgtag taaacgcggt aagcttttct gggggtgtga taaatatccc aattgtgact
     3841 ttgtggcgtg ggaccgacca atgccacaac cgtgtcctaa atgtaagggc attgtagtac
     3901 agaagaagaa agaattggct tgtatgagtt gcgattatcg taaagaacgt gacgcagacg
     3961 aagaggtgac agaagagtag gaatgtaaat gaaacaattt ctcccacgcg ctattcagga
     4021 tctcagtcaa gcgctctctc ggttgcccgg tattgggccc aaaacagcta accgtttggc
     4081 tttctacctt atcaatcgac cgaaagaaga ggtggcagag attggggccg cttttattaa
     4141 tctcaaagag aacttattgc gctgtgaggt ctgtcatacc gttagtgaaa ccaacccttg
     4201 tccaatctgt gctgatgaag agcgcgatca tgcgcggatt atggtagtgg aagagcccct
     4261 cgatatggtg gcaatggagc gtaccggtta cgagggcatt taccacgttt tgggtggtgt
     4321 tatttctcct atcaatggag tggggccacg cgacttgacg ctccaagaac tctttgatcg
     4381 tgttcagtct ggtacaatag aagaggtcat tctcgcgact gacccgagtc tcgaaggcga
     4441 ggcaaccgct tcttacatta cagagaaact gatgcaggtt gctcccaagg gattagccat
     4501 ctcacgtatt gcccgtggtc ttccggttgg aggcgatctg gaatacgctg atgaactaac
     4561 tcttcgccgc gctctcgaag gacgacaaaa ttatcgtaca aaatagtatc caggcttact
     4621 tcgctcagtg agggtctacg ctatgtcact ctcttttctc aacacactaa ctcgtacgaa
     4681 agaaacgttt gttccgatca caccggggct ggcttcgtta tatacgtgtg gcccaacggt
     4741 gtatgatttt gcccatatcg gcaatatgcg tacctatatt tttaatgaca cccttcgccg
     4801 ggttttggag atgaacgggc tgacagttca tcatgttatg aatattactg atgtgggtca
     4861 cttggtaggg gatagtgact ttgcagaaga taaagttgac gctaaggccg agaaagaagg
     4921 gctgcacccg ttagatattg cgaaaagata cgagacggct ttttttgagg atttttctcg
     4981 cctcaataat aagctaccca ccgacgttgt acgtgccact gaagctgttg attcacaaat
     5041 tgagctcatt aagcagctcg aagcacgagg ttttacctac agggatgatg acgctattta
     5101 ttttgatacc tctaaattgt ctgattatgg caaactgaca ggccaaactt tatctgaaaa
     5161 actgattggt gcccgagaag aagtagtggt tgatacacgt aaacgtaatc ctcaagattt
     5221 tgttttatgg tttttcttaa ccggacgata tgctcatcat attttgcact gggcgtctcc
     5281 ttggggagag ggatttcccg ggtggcatgt cgaatgttcg gctatctccc gtagccggtt
     5341 aggtcaacca ttcgatatcc atacgggggg agttgatcac attggaaccc accacacaaa
     5401 tgaaattgct caaagcgaag ccgcctatgg agaaccgcta gcgcactact ggcttcacgg
     5461 cgagcactta atggtggaag gaaaacggat ggggaaaagt gaaggtaatt tagttcgtct
     5521 gactaccgtc gaagaaaaag gatttgatcc gttagatttt cgttacctcg tattaaccgc
     5581 ccattatcgt tcgaggatga actttacttg ggatgcctta gtgggcgcac gtaagacact
     5641 tacgagtatc cgggagttag tggcacatga cacttcggaa aaaggggagt atgcccagga
     5701 ggaattaatc cgagccgcct tcaatgatga tttagataca ccccgtgcct tagctctatt
     5761 gcatcaagcg aagagcggag cgctctggcg gaaatttgat tgcgtgttag gtctcaacgt
     5821 cgatcatctt cctcgagaaa aacatgaagt gaacagtgaa attacagcct taaaagagga
     5881 gagagaccaa gctcgtgccc aaaaagattg ggcgaaggcg gatatgttcc gtcataaaat
     5941 agaggctttg ggttaccgag tacgcgatac ggagtcaggg agtgtgctcg aaagactata
     6001 gaaaaagtgg gcggtaataa cacaaaaatt accgcagtaa ccactttcgt gaaatatgct
     6061 taaaaagagc ggtgaaagcc gcctttttta tatgcgtaaa actgttgaca catatactcg
     6121 ccaatggtat agttttggca taaagaagag agggactccg tgcttgcacg gtagtctttt
     6181 tatttacttt gctttcttag gacagttgtg ttctggctgg gcgggtggcg gtacagtgtt
     6241 acctaatact cccgacctct cgtgatctcg catgtcgagg tctttcctgg ccagaacaga
     6301 gctgttctct ggaacaggct ctattagaac ttttacatct tctgttagtt cctcagtagt
     6361 caacctactt aaatatagat gctacgttgc cgaggccgag aggccgcagg agaagtccgc
     6421 gatgaaagaa ggaatgacac gcggccgagt accgaactcg gcggcgtggc tgttactgtt
     6481 ggtggtcttg ctgttcggag gattgtttgt atctccggcg gctaaggccg atctgttcac
     6541 ccgcaccaaa gtggtgcggc ggttcgggac cgatcgcaag atagatgtcg cgatcgacgg
     6601 tcgggcacag ttctggatgc ggttctacat cgtcaaccag atggacccgt ctgctcagga
     6661 tcgtcagggc gtcgcgccct acgattgcgc tatcgacatc gatgcacagg ccggtaagaa
     6721 cccggaagtg cgcggtggcc ggaaagagga gttcaaggac aaagtcctct cctggcgcaa
     6781 gctcttccag ggcgagggtc cgggctacga tggccgagtc cgcctccagg cggagaacaa
     6841 acgcaaccag gccaagctca agaaagacct gcaaaaggaa ggccgctccg accggtttgt
     6901 taagcggaac gtcttcaccg gcattctgaa aggtcggatt gagcccagcg aatttgtgcc
     6961 gggagacacc ctccgcatcg agtatgccgc ggatcaggat caggatgaca actacattga
     7021 tgtcgtcatt gctccttaca cgctggctca gatgctcgga atccagcaac agatgaccac
     7081 attgggcctc ctgcaagggg ggaacccgat gctcggttct caggccctcc tccccacgaa
     7141 cgtcccgctc agcgcggcga cccagtggga tcccggatcg ctcccgcacc ctcaaggggt
     7201 gcagaccccg aataacgtag ggccggatgc ccgtttccgg cgtgacggtg gtgccttcga
     7261 tggcactgcc tatcgtccgg gacagcgcaa ctcgttcccc tcgggaggac ccgcgccaag
     7321 cgggagctat ggagaccaaa ctccccacca tgtgccgccg gcgccttaca caggtgcgcc
     7381 gctctacggc tcggggacaa tggtccctcc cagcccgcct cctgcatcca ctccgaccga
     7441 gccgcctccg ggcgacccgc gtccgaatgt ggagatcgcc ccgccagcag cgcctcgtgc
     7501 tgcttctggt cgggggatcg acctggatga agaagagcgg ttcgaacgag gctcgtctcg
     7561 tcgcccggag tccgctccgg taccgatggg acagtccgca ggcgacaacg acctgcgtcc
     7621 cgtgggcacg aaaaccggaa ctctggcaac gctggagttt cagttcgtgc ttcgctcggg
     7681 atcgtttacg gagtactcct tcaagattgg agtcgtagga tatcacccgg caacggggaa
     7741 acctgtcgac ttcacgcctg gacccaacgg ccaagttttg ccgaaggtct ttcaggtcgt
     7801 cgggggaaag gcgcagtacg ccgcccagcc cggtctgatc tacgtcccat actttaccgg
     7861 gacggtgcag aaggagtggg aactcccgcc cgataagcgg gatggcatgt tcaacgaagg
     7921 taggagtccg ggctgggtcg ttcccgaagg gggacgccgg cttgttctga cacggaggaa
     7981 ctagcgatga agtggcgaca tataatcgcc gcactttctc tggtcgtgag cgccctcgtg
     8041 gcgctcccgg ctcaggccaa gaaggtgtac accgaagggc agtacggtac gtcgccactt
     8101 gggccgcgca agctggacat gaacaatggc cagttcgtga gcggtcaggc ggtggacgac
     8161 taccggcgct tcatgctcag cgaacgcgtt gctgtcgcgg ccaaggagat cggcatcgcg
     8221 ccgcacttct ggcaggtcac gaagatcgcg atcaacgacg cgaaccagga ctacgtcgtc
     8281 atccctaagg gtacgcagcg agaccttcgg gttctcattg cggacagctt tgccaagggg
     8341 atgtggacgg aaacgtccaa agggagcaag atctggtacc tccgggtcca gggcttcgtt
     8401 cccgaccgcc tcgacggcga agacatggcc ttctactccc gcgtctggaa tgaagtgcgc
     8461 gggttccagg aagacttcat gttcgcgccg ggcttccagc cccaatgcgc gggggtgctc
     8521 atataccacg aggccgccga gcagatggtc tgggtggcga tgctggagaa gtgccgcaac
     8581 ctctggtggg cgtcgcttca catctccatc cggatcctta tggaggaacg cctgattccc
     8641 gggccgcgcg gtccggaagg agaacagggt cctccgggag tacgtggtcc ggaaggtccg
     8701 attggtccgc agggacctcc aggctggaca ggtccgcgcg ggccgcgtgg ctaccccggt
     8761 ccgccgggac gagacggcga agtccgttac gtgctggggt tccagccgaa ctggagcttc
     8821 gttcctggtg ggcgccagcc cactccggca tacgtggtgg atacgaagcc gggactcatc
     8881 cccacggtag ccgcgttgat ggggttcgcc cagccgttct gggctcgagg gaacacctat
     8941 aacatcgcgg gaggtcgcgc cactggtgga aactctgtcg cgatatcgcg gcagtaccag
     9001 acatcggcaa acttcaacga gcagaacacg gatgtgactg ccaatggagg agccggtggg
     9061 gccggtggta acgccaccaa cacgacgacc gtggataatc acccggtcaa cgtgaacatg
     9121 aacaccaacg gcaacgaggt caacggcgcc aacagcaacg gcggcgccgc aggggccggc
     9181 aagtaactcg ctcaggcttc ggcttgggca cggggaaggg agaggtgact ctctcttccc
     9241 caacatctct ttctcgtttc atgtgttgtg caaagatgct caatcatgag gagagaatcc
     9301 tcatctcttt ggtcgaaaga ggtttgaata tccgtaaaat ataacggagg tggttatgca
     9361 gaaattagca atcttaccag ttctcgtttc ggcggcagcg gtagcgctca gcggcggggt
     9421 cactccggcg caggcgacca actgggaaga ctacgcagac gcaattgctg cgcagtatca
     9481 gtaccagaca tccacaaacg ttaacgtgca gaatcagtca acgaccgtgg gtgcgacaac
     9541 accaacgact tctaccggtg gtaatgcgac aaacgttacc aacgtcacaa gtcaggatac
     9601 gaatatcaac cagaacaata accagaacgt tgtctctatt gccaacgtaa acggtaacgg
     9661 ttctgcaagt gcttcgggca gtgcagcagg aacgcaaact ggaacaggta cgcaaacagg
     9721 tacacagacg gttaatccaa caccgacgcc aaccccaaca ccaaccccca ccactacaac
     9781 aggtggaaat ggtaccaacg taacgaacgt tacgtcacaa aatggcaata tcaaccagaa
     9841 taacaaccaa aacgttattc cgattgccaa cgtgaatggt aacggcacgg caactgcgtc
     9901 tggaagtgct tcgagcaccc agaatgccac gattactcaa acatcgaatc aatcggtgaa
     9961 cacaggtaat cagactggta cgccaacccc aacaccaacc agcacgacga cgacttcgac
    10021 ttcaacctcg acgtctacct cgacttcaac tggtagcaaa acaacggcaa ctgcttcggc
    10081 ttttgcttcg accctcgagc agcttcccgc tgctggagca gctccagctc ttgctggtgg
    10141 tgcattagct ggtttcggtt ccattgttgc ggttatccgc gcttgggctc gtcgccagaa
    10201 agaaatcctt aacgacctca ttggttaata atggtttttc tacaaaggct ccccgaaagg
    10261 ggagtttttg gttttgctcg agttttatag acaagcatct aaggagccct tctccgcgag
    10321 gcttgtctat aaaactctcg ctctgaatgg ggaaggaagg tataatacgt atatggaaaa
    10381 gaaacagcag ttggctgaac tattaggaat cgagaataaa aaagtgcgga ttgcagaaat
    10441 tcaaaaagat atgcagtcag ctgatttttg ggctgatcat gtgaaggctg cggaaattac
    10501 ccaagaacta aagcagctcg atgacattgt gactgaattt gaattagctg agacgccaga
    10561 agagatcgct gagttggaaa agtacacgct tctttcgggg gaatacgacg ctgagccagc
    10621 gattatgacc atttcagctg gaagtggggg aactgaagct caagactggg ctgaaatgct
    10681 tatgcgaatg tatgttcggt attccgagaa aagtggctta acggcaacga tcttagacat
    10741 ttctgagggc gaagaagctg gcattaagtc ggcaactctc gaaataaaag gtccttttgc
    10801 ttttggctac ttaaaagcag aggcaggggt acaccgttta gtacgtatgt cgccatttga
    10861 cgccgacaag gcacgtcata catcctttgc attggtccaa gtaattccaa agttaaaacc
    10921 gaaaacagtg agtattgacc caaaagacct acgggttgat gtgtatcggg cgggaggaaa
    10981 aggtggccag ggtgtaaaca ctaccgattc agcagtgcgg ttgactcatt tgccaaccgg
    11041 acttgttgtt gcggttcaaa atgaacgttc ccagctacag aacaaagaga ttgccatggc
    11101 tattttgctg tctcgattag aactgttgca aaaagagaaa aatgccgaac aagtggcgtt
    11161 cttaaaaggt gatttccgtg aagcggcgtg ggggaatcag atccgtagct atgtgctggc
    11221 tccgtaccaa ttaattaaag atcatcgtac gggccatgag tcgccaagcc cgcaagacgt
    11281 tttaaacggt gacattcaag atttcattga agccggattg cggtggatga aggaggctcc
    11341 atagctgta
//
LOCUS       DASWYB010000033         6635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_329129, whole genome shotgun sequence.
ACCESSION   DASWYB010000033 DASWYB010000000
VERSION     DASWYB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329129"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGE59_01860"
     CDS             <1..552
                     /locus_tag="VGE59_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074427.1"
                     /translation="TESIKVQNHDDAITELKKQAGDAIVLEKAIQVISQDITDGATVD
                     TIADTLEATVKGKFQVITFSQADHKELLEKLLESKIPQGQKIVTQGDGVTIDTSQFTL
                     NLVSEKRLELTNNLKAFTVTFFDEAAVRRSMIGVAPAAVKDLVKKRVPIKDVKIVVSP
                     AGWPRLPLLGSKIRVNYSYAAQE"
     gene            559..969
                     /gene="ruvX"
                     /locus_tag="VGE59_01865"
     CDS             559..969
                     /gene="ruvX"
                     /locus_tag="VGE59_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY1074428.1"
                     /translation="MEHENYLALDIGEKRIGVAVGTVIPFGRGTVDATEPQAALNHIA
                     SIIREDHITALIVGIPHVRSGDVTASQAKAHEWIMRLKEMFSLPIETVDEAYSSTQAE
                     RELRAMGVDTVKEKERIDERSAEIILAQYLEGKV"
     gene            966..1937
                     /gene="mltG"
                     /locus_tag="VGE59_01870"
     CDS             966..1937
                     /gene="mltG"
                     /locus_tag="VGE59_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY1074429.1"
                     /translation="MKGPRFSFGSFKPWLVGLSAGAFIVLLAFGIWFLSGLRGIDGPS
                     REFSIPKGERRIEIAQRLQKEGIIRSWPHFVLISYLERRVLLAGQFTLDPTDRLTTIV
                     RTLSLEEKRHQVVTIPEGWRREQIAEELAKKGVDKAGFLSLTEGLEGRLFPDTYYISV
                     TPKAQEVVSKFTTNYFDRTKELQPTKEQLILASIVEREAKRDDERALIAGIYAHRLAI
                     GMKLEADPTVQYGRDTNLVKAGTPPQEFWQSITVADYQGVVSSYNTYRTLGLPPGAIS
                     NPGIKSIEAAVHPTSTTALYFFHTADGKIITSKTYDEHLANKRQYLR"
     gene            2016..3071
                     /locus_tag="VGE59_01875"
     CDS             2016..3071
                     /locus_tag="VGE59_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY1074430.1"
                     /translation="MAFGIFNHLSHDIGIDLGTANTLVYLKGSGLIINEPSVVAINQR
                     TKEVLAIGNEAKRMVGRTPGHIVAMRPLVDGVVSDFEVTEQMLRYFINKVHTNRLRLM
                     PRPRVLIGIPYGVTEVERRAVEEAALNAGAREVYLIEEPVAAAIGARLPIQEATGNMI
                     VDIGGGTTEVAVISLGSIVASRSLRIAGDELNQDIINYVRDEFNLLLGERTAEEVKIA
                     AGSALPLKNRIKVVLRGRDLISGLPKMIETDDEPIRRALQKSVNSIIDAVRSTIEETP
                     PELVADIMDRGIVLAGGGALLRGLNIIIATATGTTVHIADDPLTAVARGTGVVIEDLS
                     GLREVLLPTQSKRLDRV"
     gene            3088..3861
                     /gene="mreC"
                     /locus_tag="VGE59_01880"
     CDS             3088..3861
                     /gene="mreC"
                     /locus_tag="VGE59_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY1074431.1"
                     /translation="MYNTWRKVQRPLIGLLFAILVLFIPPVRNRIIVTLSPLVVTTRH
                     VGESVFDFAQLIASIPTLSSENGSLRSHINELQALEVKQKELAHENELLRKELNLSPA
                     QSDTLIAAQVISRASSVSQQQIVINKGTKDGFTTGMAVVAQGYFIGQVEEALESTSRI
                     DLITSVRSLVPVVLQTSRSVGLLKGGAEGLVIDEIPRDVTIDSGEALVTANLGNVVRG
                     GLPVGTVSAVLSGKSDVFQSARIHSPIDFSRLEVVFGVK"
     gene            3861..4298
                     /locus_tag="VGE59_01885"
     CDS             3861..4298
                     /locus_tag="VGE59_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074432.1"
                     /translation="MTLFAFFVALIDISIGANFVLWGGGGYIFPAFIILLAASHNWSQ
                     LIIWISIYLLTGIFIPLSPLPFFILLLLCLVAVFLFRRVVAPDSLLVQACFALVLLAV
                     YIAASYYSQGVRLSLNYIGSLMLTLPLIVIWFLVRNQRRRRLS"
     gene            4424..6205
                     /gene="mrdA"
                     /locus_tag="VGE59_01890"
     CDS             4424..6205
                     /gene="mrdA"
                     /locus_tag="VGE59_01890"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY1074433.1"
                     /translation="MDRPNYLLARIGILFLGLALVFRLTNLQIAEGNENRYLAEGNRI
                     RQQVTVAPRGAIVDRKGELLVTNEAGYSLDLVPSDLPRQRAEREKVLQEVGTLTGSSL
                     ETLSKALNQVGLFSLDPIIIQPNLTREQALIYKIKLSSLPGVRVSYLPTRKYDETIGL
                     SHLLGYTARMTQADLDKHPDYPRNSLIGRLGIEGAYDPQLRGEVGIHELEVNASGQLQ
                     RSLKNTPPKVGATLHLTLDRGLQAELVAALQEAMAKNGSQNAAAIAMNPQTGAILASV
                     SLPSYDNNLFAGGISQEDYNRLNTDPNKPLLNRVVDGLYPPGSTIKPFVAAAALQEKT
                     IAPNTQLDTSAGAIEIGQWRFPDWKTHGVTDVKRAIAESNNVFFYSLGGGWNKISGLG
                     VERLERYYQMFGLGQPTDTDFDQGKAGLVPNPEWKKKVKKETWYIGDTYHIAIGQGDL
                     LATPLQMVRAVSSLVNGGRLVTPHLVSTVESSESGVREDLTFPESPLPLSAETFQTVR
                     EGMYQTVNSDTGSARVLRNLPFSSGGKTGTAQFGTDKKTQAWYIGFAPYENPEIAITV
                     IVEGGGEGNAIAVPVAGRAFAYYMSHR"
     gene            6388..>6635
                     /locus_tag="VGE59_01895"
     CDS             6388..>6635
                     /locus_tag="VGE59_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY1074434.1"
                     /translation="MAMKSITLTKEGYEQLVKELKELKEVKRPEVISRIKSAQELGDL
                     SENAEYQTAKEDQSFVEGRIQELEIILKTAKVVERQHST"
BASE COUNT         1743 a         1398 c         1660 g         1834 t
ORIGIN      
        1 accgagagta ttaaggttca aaatcatgac gatgcaatca ctgagttaaa aaaacaggca
       61 ggcgatgcaa ttgtacttga aaaggcaatt caggttatat cgcaagacat taccgatggg
      121 gcaacggtgg atactattgc cgatacgctt gaagcgacgg taaaagggaa atttcaagta
      181 attacatttt ctcaggcaga tcataaagaa ttacttgaaa agttacttga gagcaaaatt
      241 ccccaaggac aaaagatcgt tacgcaaggc gatggagtca caatcgacac atctcaattt
      301 actcttaact tagtttcaga aaagcgttta gaactcacaa ataacttaaa agcgtttacg
      361 gtcactttct ttgatgaagc tgctgttcgt cgttcgatga ttggtgttgc gccggctgcg
      421 gtaaaagatc tggtaaaaaa acgcgtaccc attaaagatg tcaaaattgt tgtaagcccg
      481 gctgggtggc ctcgtctccc tcttcttgga agtaaaatac gtgtgaatta cagttatgcg
      541 gctcaagagt aaataagtat ggagcatgag aattatctgg ccctagacat cggagagaag
      601 cgtattggtg ttgctgttgg aacggttatc ccttttggcc ggggtacggt cgacgcaact
      661 gaaccgcagg cagcattgaa tcatatcgcc agtattattc gagaagatca cattacggct
      721 ttgattgtgg gcattcctca tgttcgaagt ggtgacgtaa cagctagtca ggccaaagcg
      781 cacgagtgga ttatgcgctt aaaagaaatg ttttctctcc ctattgaaac tgttgatgag
      841 gcctatagct ctacacaagc tgaacgagaa ttgcgagcga tgggggtgga tacagttaaa
      901 gaaaaagaac ggattgatga acgttcggcc gagattatcc tggctcaata tttagagggg
      961 aaggtatgaa gggtcctcgt ttttctttcg gttcattcaa gccatggttg gtcgggttgt
     1021 ctgcgggtgc ttttattgta cttctcgcat ttggcatatg gtttttgagc ggactgcgag
     1081 gtatagacgg tccttctcga gaattttcta ttccgaaagg agagcgtcgg attgaaattg
     1141 cgcaacggct tcagaaagaa ggtattattc gttcctggcc acactttgtg ctgatatctt
     1201 atttagaacg gcgcgttctt ctggcgggac aatttaccct cgacccgact gatcgcttaa
     1261 cgacaatcgt acggacgctt tctctggagg agaaaagaca ccaagttgtt actatccctg
     1321 aagggtggcg tagagaacag attgcggagg aattggcgaa aaaaggagtc gacaaagccg
     1381 gatttctttc tttaactgaa gggcttgaag ggcgtctttt ccctgatacg tattacatct
     1441 ccgttacgcc caaagcccag gaagttgtga gtaaatttac aacgaattat tttgatcgga
     1501 caaaagaatt gcaaccaacc aaagaacaac tgattcttgc ctcgatcgtc gagcgagaag
     1561 ccaagcgtga tgatgaacga gctttgattg caggaattta cgctcaccgt ctcgctatcg
     1621 ggatgaaatt ggaggctgat cccaccgtcc aatatggaag agataccaac ctcgttaaag
     1681 ccggtacgcc acctcaggag ttctggcagt ctataaccgt tgctgactac caaggcgttg
     1741 tatcttccta taatacatac cgtacgcttg ggctccctcc gggtgctatc tcgaacccag
     1801 gaataaagag tatcgaagcc gcggtgcatc ctacttcgac aacagctctg tacttttttc
     1861 acacggcgga tggaaaaatt attacgagca aaacgtacga tgaacatctc gcaaataagc
     1921 gccagtacct tcgttaaggt actgagagga tgatgtttta ggttcatttt tcaatttaag
     1981 ccttttctgt tacactccag aaggtctcac tctttatggc atttggaatt tttaatcatt
     2041 tatcacatga tatcgggatt gatctgggca ccgcgaatac gctggtgtat ttaaaaggga
     2101 gtggattaat tattaatgag ccttcagtag tcgcaattaa ccaacgaaca aaggaagttt
     2161 tggcaattgg gaatgaagcg aaacgaatgg ttggacgtac gccgggacat attgttgcga
     2221 tgcggccact tgtcgacggc gtggttagtg atttcgaagt taccgaacaa atgcttcgtt
     2281 attttattaa taaagttcat acgaaccgcc tccggcttat gccacgtcca cgggttctta
     2341 ttgggatccc ctacggtgtg accgaagttg aacgtcgcgc tgttgaagaa gcggcactca
     2401 acgcaggggc tcgcgaagtg tatttaattg aagaacccgt tgctgcagcc attggagccc
     2461 gattaccgat ccaagaagcc acaggtaata tgattgttga tattggtggt gggacgactg
     2521 aagtagccgt aatctcgtta gggagtattg tggccagtcg ttcgctgcgt attgcgggag
     2581 atgagcttaa ccaagatatt attaactacg ttcgtgatga gtttaacctt ctattaggcg
     2641 aacgtacggc agaagaagtg aaaattgccg ctggcagtgc cttgccgctc aagaatcgca
     2701 taaaagtagt tttgcgtggc cgcgatctta tttctgggtt gccgaaaatg attgagactg
     2761 atgacgaacc gattcgtcgg gcgttacaga agtctgttaa ctcaattatc gatgcggttc
     2821 gttctaccat cgaagagact cctccggaac tcgtggcaga tatcatggat cggggaattg
     2881 tcttggccgg tggcggggcg ctattacgag gccttaatat tattattgcg accgcaacgg
     2941 gaaccacagt gcacattgcc gacgacccgc ttacggcggt agccagaggt acgggcgtgg
     3001 tgattgaaga tctcagtgga ttacgtgagg ttttgctccc gacccaatca aaacggttag
     3061 atcgcgtgta agcagagaag gaggagtatg tataacacct ggcgaaaagt tcaaaggcct
     3121 ttgattgggc tactttttgc aattttggtc ttattcattc cccccgttcg aaaccgtatt
     3181 attgtcacac tttctcctct tgttgtgaca acccgacatg tgggcgaatc tgtgtttgat
     3241 tttgcgcagt tgattgcttc aatcccgact ctttcttctg aaaatggttc cctcaggagc
     3301 catattaatg aattacaagc actcgaggtg aagcaaaaag aacttgctca tgaaaatgaa
     3361 ctcctccgta aagagctcaa tctctcacct gctcagtcgg atacacttat tgcggctcag
     3421 gttatttctc gtgcctcttc cgtgagtcag cagcaaattg taataaataa aggaacgaaa
     3481 gatgggttta cgaccggaat ggccgtggtg gcgcaaggct actttattgg ccaagttgaa
     3541 gaagctcttg aatcaacgag tcgcatcgac ctgattacaa gcgttcgatc cttagtcccc
     3601 gtagtgttgc aaacttcacg gtcagttggt cttttaaaag ggggtgccga ggggttggtt
     3661 attgatgaaa tccctcgcga cgtgacgatt gattctggtg aggcgttggt aacagccaac
     3721 ttaggtaacg tggtgcgagg aggattaccg gtggggacgg tatcggcagt cttatcaggg
     3781 aaaagtgatg tttttcagtc ggcccgtatt cactcaccga ttgattttag ccgcctcgaa
     3841 gttgtctttg gggtgaaata gtgacgcttt ttgctttttt tgtcgcattg attgatatct
     3901 ccataggggc aaactttgtg ctgtggggtg gcggtggtta tatctttccc gctttcatca
     3961 ttcttttagc ggcatcgcat aactggagcc aattgattat ttggatcagc atttatcttc
     4021 tcaccggtat ttttattccg ctttctcccc tccctttttt cattcttctg ctactttgcc
     4081 tggttgccgt cttccttttt cgccgtgtgg ttgcgcccga tagtctactt gttcaagcct
     4141 gttttgcgct cgttcttttg gcagtctata tcgcggcctc ctattacagc caaggtgttc
     4201 gattatcact taactatatc ggcagcctta tgttgacatt gccgcttatt gttatatggt
     4261 tcttggtacg taaccagcgc aggaggaggt tgtcatagcg cgcagaaaag gtttttttgg
     4321 ttatcagtca agcatcttca aattactttc tcgtccacga gaagactggt ttgatgcatc
     4381 tggtttttta ctcgagccgc aaggggttct tcccgacgag gaagtggatc ggccaaatta
     4441 ccttttagcc cgtattggca ttttatttct cggtcttgct cttgtgtttc gtcttacgaa
     4501 tctgcaaatt gcagaaggaa atgaaaaccg ttatttggca gaaggtaacc gtatccgcca
     4561 acaggttact gttgctccgc ggggagcaat cgtggatcga aaaggtgagc ttttagttac
     4621 gaatgaggcg ggttatagtt tagacttagt tccctccgat ttgccacggc aacgagcgga
     4681 gcgagaaaag gttttgcaag aggtgggtac actgactggg tcttcgttgg agacgttatc
     4741 caaagcactt aatcaggtgg gacttttttc cttagatcca attatcatcc agccaaatct
     4801 tactcgggaa caagcgctta tttataaaat aaaactttct agtttgcccg gcgttcgtgt
     4861 atcgtatctg ccgacccgga aatatgatga aacgattggt ctttcccatc tgctgggata
     4921 tacagcccgg atgactcaag cggatctcga taaacaccct gactatcctc gaaactcatt
     4981 gattgggagg ctggggattg aaggagcgta tgatcctcag ttgcggggtg aggtgggaat
     5041 acatgaactg gaagtgaatg cgagtggtca acttcagcgt tcccttaaaa atacaccgcc
     5101 caaagtcggc gcgactctcc atttaaccct cgatcgtggg ttacaagcgg agttagtcgc
     5161 ggctctccaa gaagccatgg ctaaaaatgg gtctcaaaac gccgctgcca ttgcaatgaa
     5221 cccccaaacc ggagctattc tcgcgtctgt ttcattgccg agttacgaca acaacttgtt
     5281 tgcgggcggt atctcgcaag aagattacaa ccggcttaat actgatccga ataagccact
     5341 cttgaatcgt gtcgttgatg ggttataccc tcccggctca acaattaagc cctttgttgc
     5401 agctgctgct ctccaagaga aaacaattgc gcctaatact cagcttgata cttcggccgg
     5461 ggcaatcgag attggtcagt ggcgttttcc tgactggaag acacatggag taacggatgt
     5521 gaagcgagca attgctgaat caaataatgt tttcttttat tcacttggtg gggggtggaa
     5581 caagatttct ggcctgggcg tggaacgtct tgaacgttac taccaaatgt ttgggcttgg
     5641 gcaaccgacg gatacggact ttgaccaagg aaaagctggt ttagtgccta accctgaatg
     5701 gaagaaaaaa gttaagaaag aaacatggta tatcggcgac acctaccata ttgccattgg
     5761 tcaaggagat cttctcgcaa ctcctttaca aatggttcga gccgttagta gccttgtgaa
     5821 tggaggccgg ttggttaccc ctcaccttgt ttcaactgtt gaatcctcgg aaagtggtgt
     5881 gcgagaagac cttacttttc ccgagtctcc ccttccctta tctgcggaaa cctttcagac
     5941 ggtgcgagag ggaatgtacc aaacggttaa ctcagatacg ggttcggcgc gggtgctccg
     6001 taacctgcca ttttcttcag gaggaaagac gggaactgct cagtttggga cggataaaaa
     6061 aacacaagcc tggtatattg gctttgcacc ttacgaaaat ccagagattg ctattaccgt
     6121 tattgtcgaa ggaggaggag agggaaatgc cattgcggta cctgttgccg gacgtgcgtt
     6181 tgcctattat atgagccatc gttaataaat aaaaaataca ttaataatca ttgacaacat
     6241 tcttgggagt tgttatatat aagtcagcag atcaggtaat tcagtttctg aggaggtggc
     6301 gaaagccgct gttttgttga aaagggtgta aaactgcccc tagaaaagag taagaagttg
     6361 acccgaaacg ggaaaaggag ccttatcatg gctatgaaat caattacttt aaccaaagaa
     6421 ggatacgagc agctcgtgaa agagctcaaa gaattgaagg aagtaaaacg tcctgaagtt
     6481 atatctcgta ttaaaagcgc acaagaacta ggcgacctct cagaaaacgc agagtatcaa
     6541 actgcgaaag aagaccagtc gttcgtagaa ggacgtattc aagagttaga aatcattctt
     6601 aagaccgcta aagtggtgga acgtcaacat tctac
//
LOCUS       DASWYB010000034         9450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_182514, whole genome shotgun sequence.
ACCESSION   DASWYB010000034 DASWYB010000000
VERSION     DASWYB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182514"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VGE59_01900"
     CDS             complement(<1..540)
                     /locus_tag="VGE59_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY1074435.1"
                     /translation="MSIVTKTKDNHSSVDLDRFRQKKKRRWLFVLLGLILAVAVWFAS
                     TIFIAYNRATTTNTTGGSSILQGAGKKEEKGTINILLIGIGGASHPGGTLADTIMIAS
                     IDTTTQTVSLVSLPRDLYVTLPDGGGKEKINAAHSYGEKNWKTKGGGPAMLKQVVSQV
                     TGVPIHYFVRIDFDGFKKIV"
     gene            complement(595..981)
                     /locus_tag="VGE59_01905"
     CDS             complement(595..981)
                     /locus_tag="VGE59_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074436.1"
                     /translation="MQRVLIFMISNNEQYVGLWQPHHDDYWHTVVIEPGARADGQIFT
                     AVETLLSLHHLPLKSITHIGVMPGPASYTQLRIYVATANALAWALHLPLFAVPTGSSL
                     PEDIPSLLTSAKRDVPIEPIYPSIID"
     gene            complement(986..1453)
                     /gene="tsaE"
                     /locus_tag="VGE59_01910"
     CDS             complement(986..1453)
                     /gene="tsaE"
                     /locus_tag="VGE59_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY1074437.1"
                     /translation="MYQTYIFLRSLHDTEKFANELVGVLTRPAVIALTGDLGSGKTTL
                     SQYIAKALGVREFLPSPTFTLMNEYSLPDSQVLIHSDLYRLKDATEVRELGIEDYFVD
                     ARAVTVVEWADRAESIFPSHTIWIHIDTKSEERIAVVRGGTQAFWTHFQEKGI"
     gene            complement(1462..3240)
                     /locus_tag="VGE59_01915"
     CDS             complement(1462..3240)
                     /locus_tag="VGE59_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY1074438.1"
                     /translation="MRQTGLITFIVIILVLPLFCSRAFAADEPFLSLASQENFALPID
                     QIALPSAKAFSLKVCFSMVEENPPLADCGGQTWNGKEWLSPSAPWREFPTSNTAYAVT
                     GRYIKEQPVGYLHIVGRESPDGTKSTVLATWRVEPRLNHHSVRLRAFKQTGEVIESGY
                     VRVYGRSIFMVPLVEGWTSFSLPEEEATLEVLDAAGTIQAPAKNYIFLTPNASYTLVI
                     NAPPYQSYMPALIQPESVLSGSNITSYLRLARPYSGRVVWKRNGIVLAESGQELVWRE
                     TQSGVYDLEAVVEETGEYVRKSVAVMPVTQVKITQLVPNPDGVDANGKEEIRIKNGHV
                     DSVSLNGWRLRRRNASTFIALSGEILGRSELVIPSSNKLVNSGETYDLYNNSGDLIDT
                     VTYPAVEAGEVLTRDGYVWQSNKTAQLGTSTEIVSPELADVVGRVTRPRGRTIDLVTS
                     KGEATHVVVHTSYVSSRPKLHVGDTIHVTGRWKQSRSGLYVSVRIGDIFELLELATPT
                     RKRQPSIFKKTSNRSVEESMPPPRVSTDSLNGVPRFSPAMLFTSSVFTSWDTPPLGTH
                     VTRFWLVTLFSLGIVLVLPFSRPIPV"
     gene            complement(3247..4290)
                     /locus_tag="VGE59_01920"
     CDS             complement(3247..4290)
                     /locus_tag="VGE59_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY1074439.1"
                     /translation="MHLRKFVPNLKDDESVYVFARPYFLSFLPWITLGIAMLIVGVIM
                     VIIAATTFPEIQQNALANNIFIIMSSAFFLLILPFLTVAFIDFYYDVHIVTDRRLIDI
                     DQHALFKREINELALEEVQDVTSIHQGVLGNIFDYGLVSIETASSNQKFEFQYIRHPN
                     EIAAIILDLADQAKMRIERGPGDIVSPRSEIKAVINDKIYSNLRELETMGAVVPIEVG
                     SRVGKDMEGHIPSSGADPKRIHTVELAGVVQEQEVKAKPVHHPPEQVEVIGIDPKRIE
                     DQKAEVAEDYPSLEDEKETWEESQEDTIQESEPEELPASPPPAVAPPKKAPAPADPDE
                     LDIIIDDPNASKK"
     gene            complement(4298..4483)
                     /locus_tag="VGE59_01925"
     CDS             complement(4298..4483)
                     /locus_tag="VGE59_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074440.1"
                     /translation="MWILFIIYFIYLLLYGLISYAILFHLVKYQIEGDKSKMVLMLYL
                     VLSAIIIIGSIVFLRPF"
     gene            complement(4593..5138)
                     /locus_tag="VGE59_01930"
     CDS             complement(4593..5138)
                     /locus_tag="VGE59_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074441.1"
                     /translation="MSGPPRRRGAAAKAKAGVQAANRALRRRCAECDEFVTKGARFCP
                     NCGGEEFVEPKTPEFLTTPSGKLTALVVGGILAFLIWKYLIPEVLFYIGYCWMWTKYF
                     VVQTLVIIASYAMLAFFCLGILSAFLWVLPGGPGLVKVLWKGVFGFIGKIFTWFFGRF
                     GIDTKEKKKKKEGDKKDEDED"
     gene            complement(5279..6571)
                     /locus_tag="VGE59_01935"
     CDS             complement(5279..6571)
                     /locus_tag="VGE59_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074442.1"
                     /translation="MSVSAVNFVRRPLKQITSPISGTVDITRVRRIVDSRRFQKLRHR
                     KQLGNAQVAYPDAGHPRFGHCIGAGKWAERITRRWLEWGLISPQDAIDGETFGFLHDI
                     GHGPMSHITDPLVDDDHEGRGVTYLRELAPEIADSGADPERMVRMMLRQDPLAGVVLH
                     TPLGADKLDYLARDGHHSGLFGMPNEALFMDYVYYLDHQVVADIKIYESVQLLMQLYR
                     TMYSSLYFRTVVLLSERYIQRMISILLGHTGCDPVFSSEELVEMVDVELEYQLRRNPI
                     VQGHYERHLARQQPMPAIIYQLDPFHKHNWSGDHPVLHTPIEESVLTDKRLRDPRVLL
                     RIEREIEALMGLSEFSVLAVAPVLPRRFEPQNILFLTGDGAVPLKEISPAWWNAQVEE
                     ARRNAILHIGVDREHCALLAQAEVARDVRQIVASVLAV"
     gene            complement(6635..7186)
                     /locus_tag="VGE59_01940"
     CDS             complement(6635..7186)
                     /locus_tag="VGE59_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074443.1"
                     /translation="MKKVESVRREYIVTLKYRSSKADVLLNFLIAHSDTLRLTRSTWW
                     QLWPLPWKKVGTEKDPIFRRQLEFVSKEFIRKEVTNKKYNDIPQGNARYEHYYFKLQG
                     KVKEVLSAAGLFMNNYDEEFFFGFEHPAFYKKGEMIAAFMSYTYTAILLLTEKEREEL
                     ERLGLQVASTILLEGTDSCLIAQ"
     gene            complement(7220..7522)
                     /locus_tag="VGE59_01945"
     CDS             complement(7220..7522)
                     /locus_tag="VGE59_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074444.1"
                     /translation="MHPRYILGIGNAHVTQYESIALNQFHGKAIDKTGPSSYALSIPR
                     TGIPISTKGTKSFCNWTMYKLAQFIEQEGLDAELFFLHTARSDKSRIRDEIRKFST"
     gene            complement(7667..7969)
                     /locus_tag="VGE59_01950"
     CDS             complement(7667..7969)
                     /locus_tag="VGE59_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074445.1"
                     /translation="MGAKKGLLRGNKVSHSHSSMIAPAEKLVAVMKDLPEVKKVVLGI
                     IKRVKCSPGVKVLQEGPAILRVTLRGSASMQILWVYLQDPLDIKKVTLRIEDIFSP"
     gene            complement(7969..8430)
                     /locus_tag="VGE59_01955"
     CDS             complement(7969..8430)
                     /locus_tag="VGE59_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074446.1"
                     /translation="MIFLIISIVVPLLIYFLFPPFLAYLTGKQVRPSGLLLVACFLYF
                     ISWYLPSPLIEGRNTSIMTHVVGGGLFCGFLWLDIKTTLGLKFNIWFEVITLFALVSA
                     FGVANELFELLIVQLKLVDLHLTDTSWDLVANTLGAVIFWVGYRLTRMGKK"
     gene            complement(8462..8869)
                     /locus_tag="VGE59_01960"
     CDS             complement(8462..8869)
                     /locus_tag="VGE59_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074447.1"
                     /translation="MNDIHKAAGIIIQNRKLLVERSIGKEFFISPGGSIEEGETPETC
                     LIRELHEEFTIKVQESDLEFFGEFTSEAVNHPGRMVHLKVFVVTQWESEPTPSSEVEE
                     LAWITSAPPRGMKLGSIFAHEILPRLKALNLID"
     gene            complement(8869..9243)
                     /locus_tag="VGE59_01965"
     CDS             complement(8869..9243)
                     /locus_tag="VGE59_01965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY1074448.1"
                     /translation="MDMLLPPATGSIFYEDKKVIAHLASFPFTAGHSLVVWKEPVSDL
                     HLLGPNEYRHLMTVVDNVRNALRQAYKVEKAYLMYLDEAKHVHWHIVPRYNEQGFSLL
                     THNPTEITDFSMVPTLSKALTT"
BASE COUNT         2695 a         2313 c         2027 g         2415 t
ORIGIN      
        1 tacaattttt ttaaagccat caaaatcaat ccgtacaaag taatggattg gtacgccagt
       61 aacttggctc acaacctgct ttaacatcgc agggccacca cctttggttt tccagttttt
      121 ctcgccatac gaatgagcag cgttgatttt ctcttttccc ccaccatccg gcaatgttac
      181 atagagatca cggggaagag aaacaagtga aacagtctga gtcgtggtat caatagatgc
      241 aatcataatg gtatccgcca aggttccccc ggggtgggaa gcaccaccga taccgatcag
      301 gagaatatta attgtgcctt tttcttcttt tttaccggca ccctgcagga tcgacgaccc
      361 acccgttgta ttggttgttg tggcacggtt ataggcaata aagatcgtcg aggcaaacca
      421 aacagctacc gccaaaatga ggccgagtag cacaaaaagc caccgtcgtt ttttcttctg
      481 acggaagcga tcgagatcaa ctgaagagtg attgtcttta gttttcgtta caatgctcac
      541 gatataccta caatagcgaa aatttacccc tttgcctagc tgttaaaccc gtgtttagtc
      601 gataatagaa ggataaattg gctcgatagg aacatcccgt ttggcggacg tgagtaaact
      661 tgggatatct tccggaaggc tactccccgt tggtacggca aaaagcggga ggtggagcgc
      721 ccacgccaga gcattagcag tggcaacgta aattcttaac tgtgtatatg aagccggacc
      781 cggcatgaca ccgatatgcg taattgattt gagcggtaag tgatgaagtg agagcagcgt
      841 ctcaactgct gtaaaaattt gtccgtcggc gcgagcaccc ggctcaatta caacggtatg
      901 ccaataatca tcgtggtgtg gctgccagag tccgacatac tgttcgttat tactgatcat
      961 aaaaattagt acgcgctgca tagatttaaa tccccttttc ctgaaagtgt gtccaaaacg
     1021 cttgcgtacc gcctcgaaca accgcaatcc gttcctcaga tttggtgtca atatggatcc
     1081 atatcgtatg agagggaaat atcgattcgg cacgatcagc ccattcaaca acggtaacgg
     1141 cacgagcatc gacaaaataa tcctcaatac caagctctcg cacttcagta gcatccttta
     1201 aacggtataa atccgagtga attaaaacct gagaatcggg taaggaatat tcgttcatga
     1261 gggtaaacgt tggacttggt aaaaactccc taacgcccaa ggctttggca atatattggc
     1321 tcaaggtagt tttgccactt cctaaatctc cggtcagcgc aattacagcc ggacgggtta
     1381 gtacgcccac cagctcattg gcaaattttt ccgtatcgtg gagcgagcgc aagaaaatgt
     1441 aagtctgata catggttctt attatactgg tatcggacga gagaagggaa gaacaagcac
     1501 aatgccaagc gaaaataacg tcacaagcca aaagcgggtt acgtgcgttc ctagtggggg
     1561 agtgtcccaa gaagtaaaaa ccgaagaggt aaacagcata gctggagaaa accgtggaac
     1621 cccattcaac gagtcagtcg atactcgagg aggtggcatg ctctcctcca cactacgatt
     1681 actcgttttt ttgaatatcg atggctggcg tttacgagtc ggagtagcca actcaagaag
     1741 ctcaaaaata tctccgattc gcacactaac atacagcccc gaccgtgact gcttccagcg
     1801 accagtcaca tgaattgtat ctcccacatg aagcttggga cgcgaagaaa cataactcgt
     1861 gtgcacaaca acatgcgtgg cttcaccttt agaagtaacc agatcaatag tccgacctcg
     1921 aggacgagtt acccgcccta caacatcagc caactccggc gacacaattt ctgtcgatgt
     1981 tcccaactga gcggttttat tagactgcca gacataacca tcacgtgtta aaacttcgcc
     2041 agcctccacc gccggatagg taaccgtatc aattagatca cccgaattat tataaagatc
     2101 gtacgtctcc ccactattaa caagcttatt agacgatggg atcacgagtt cgcttcttcc
     2161 gagaatttca ccgcttaaag caatgaaggt cgaagcattt cgtcggcgca atcgccagcc
     2221 attcagagat acagaatcga cgtgaccatt cttaattcta atctcttctt ttccgttagc
     2281 atcaacgcca tcaggattgg gaactaactg cgtaatcttc acctgggtta cgggcataac
     2341 ggcgacactc tttcttacat actcccccgt ctcttctacc actgcttcta ggtcataaac
     2401 gccagactgc gtttcgcgcc aaaccaactc ctgacccgac tcagcgagaa ctatcccgtt
     2461 acgtttccaa accacacgac cagagtaagg ccgtgcaagc cgaaggtatg aagttatatt
     2521 actcccactc aggactgact cgggttggat cagggcaggc atgtacgact gatacggtgg
     2581 cgcatttatt actaatgtat acgaggcatt gggtgtgaga aatatgtagt tttttgcagg
     2641 cgcttgaata gtgccagctg cgtcaagaac ctcaagagtc gcctcttcct caggcagaga
     2701 aaacgaggtc caaccttcga ccaacggcac cataaaaatg gaacggccat acactcgaac
     2761 atatcctgat tcgatgactt cgccggtttg tttgaatgct cgtaaacgta cggagtgatg
     2821 gtttaaacgt ggctcaactc tccatgttgc cagaaccgtg cttttggtac cgtccggact
     2881 ctcccgaccg acaatatgca aatatcctac cggctgttcc tttatgtatc tacctgtcac
     2941 tgcgtatgca gtattgctcg tgggaaattc tcgccacggg gcagatgggc ttaaccattc
     3001 ttttccattc cacgtttggc ctccacaatc agcgagtggg ggattttctt ctaccattga
     3061 aaaacaaact ttaagagaga atgctttagc cgaaggaaga gcaatttgat caatcggaag
     3121 ggcaaagttt tcttgagaag ccaacgacag aaaaggttca tcggctgcga aggcacggga
     3181 acaaaaaagc ggcaggacaa ggattattac gatgaatgta ataagccctg tctgccgcat
     3241 acgttgttac ttctttgatg cgttggggtc gtcgattatg atatcaagtt catctggatc
     3301 ggcaggagca ggcgctttct ttggtggagc tactgctggg ggaggagagg ctggtagttc
     3361 ctcgggttcg ctctcttgta tggtgtcttc ctgtgactct tcccatgttt ctttttcatc
     3421 ttcgagagag ggataatcct cggccacttc tgccttttga tcttctatac gtttgggatc
     3481 aatcccgatt acttcgactt gttctggtgg gtgatggaca ggctttgctt tcacttcctg
     3541 ttcctggaca actcccgcaa gttcaactgt atgaatacgt tttggatctg caccgctgga
     3601 tgggatatgc ccctccatat ctttacctac ccggctacca acctcaattg gcacaactgc
     3661 acccattgtc tctaattccc gaagattact ataaatctta tcgttaataa ctgcttttat
     3721 ttcacttctc ggggaaacaa tatctcccgg accacgttca attcgcattt tggcttgatc
     3781 ggcaagatca agaataatag ccgcaatttc gttgggatga cggatgtact gaaattcgaa
     3841 cttctgattc gaagaagcgg tctctatcga gaccaaacca tagtcaaaaa tatttcccaa
     3901 gacaccctga tgaatggaag ttacgtcttg cacttcttct aatgctagct cattaatctc
     3961 ccgcttaaaa agggcatgct gatcaatatc gatcagtctt cgatccgtta caatatgtac
     4021 atcatagtaa aaatcgataa acgcaacggt gaggaaagga agaattagca gaaaaaaagc
     4081 actgctcata atgataaaaa tattgtttgc cagagcgttc tgttgaatct caggaaacgt
     4141 tgtcgccgcg ataattacca ttatcacccc cacaatgagc atcgcaatac ctagcgttat
     4201 ccagggcaag aaggagagaa agtaaggtcg ggcgaaaacg tagaccgact catcatcttt
     4261 tagattagga acaaatttac gtaaatgcat aaaactccta aaaaggacgt aaaaatacaa
     4321 tcgagccaat aatgataata gctgaaagta ccaggtaaag catgagtacc attttgctct
     4381 tatcaccttc aatttgatac ttcaccaaat gaaataaaat ggcgtaactg attaacccgt
     4441 agagtaaaag gtaaataaaa tagatgataa acagaatcca catatgcagt tactatacca
     4501 tttctcctga agcaattcca taagttaata aaaaaagcgc ctcccggaaa ccgggaggca
     4561 aattgcacgc tgccccttat ggtgggacgt cgctagtcct cgtcctcgtc cttcttgtcc
     4621 ccttccttct tctttttctt ctccttggta tcgatcccaa aacgaccgaa gaaccaagta
     4681 aagattttac cgatgaagcc aaagactccc ttccagagga ccttcaccag ccctgggcca
     4741 ccaggaagta cccataggaa agctgagagt atccccagac agaagaaggc aagcatcgca
     4801 tagctcgcga tgatcaccag agtctggaca acaaagtact tggtccacat ccagcagtag
     4861 ccgatgtaga acagtacctc cggaatcaag tacttccaga tcaggaaggc caagattccg
     4921 ccgactacga gtgcggtgag ctttccactc ggagttgtca gaaactccgg cgttttcggc
     4981 tccacaaact cttcgccgcc acaattgggg caaaaccgag cccccttggt gacgaactcg
     5041 tcgcactcag cacaccgtct ccgaagagcg cggtttgctg cctgaactcc ggcttttgcc
     5101 ttagcggcag cgccccggcg gcgcggcgga ccactcatcg aattcccctt ttgcttgcag
     5161 tgcaggtaca agcagctaga ctataccaca aaatgtgtaa aaagtccatg gtctcgtata
     5221 aaaaaggccg gtgcgaccaa agccgcaccg ggttagaatg gattctcttg tcgctcatct
     5281 acacggcaag aaccgaagca acaatctggc ggacatctcg cgctacctct gcctgcgcca
     5341 ataaagcaca gtgctctcgg tctaccccga tatggagtat cgcgttccgc cgagcttcct
     5401 cgacttgagc attccaccac gcaggactga tttcctttaa gggaactgcg ccgtctcctg
     5461 taaggaaaag aatgttctgg ggctcaaatc gccgtggaag tacaggcgcc accgcgagaa
     5521 ccgagaattc agagaggccc atcagggcct caatctcccg ttctatacgt aagagcaccc
     5581 gtggatctcg taaccgctta tccgtcagca cactttcttc aatcggcgtg tgcaagaccg
     5641 gatgatctcc cgaccagtta tgcttgtgaa acgggtcgag ctggtagata atcgccggca
     5701 tgggttgctg acgagcgaga tgcctttcgt agtggccttg tacaattggg ttgcgcctga
     5761 gctgatattc caactcgaca tcgaccatct cgacaagctc ttcagagctg aacacagggt
     5821 cacaccctgt atgccccaga aggatactga tcatgcgctg gatgtaccgc tccgaaagca
     5881 ataccaccgt acggaagtac aaagaagagt acatcgttcg gtaaagctgc ataaggagct
     5941 gtacggattc atagatcttt atgtctgcca ccacctgatg atccaagtag tagacataat
     6001 ccatgaacaa cgcttcgttt ggcatcccga ataacccgct atggtggcca tctcgcgcca
     6061 ggtagtccag tttgtctgca cccaatggcg tatggagaac cacaccggct aacggatctt
     6121 gccggagcat catccgaacc atccgttcgg gatctgcacc tgagtcagct atctctggag
     6181 ctaactctcg gagatatgtg actcctcgcc cttcatggtc atcatccacc aacggatcgg
     6241 tgatgtgaga cattggccca tgaccgatgt cgtgtaaaaa accgaaggtc tcaccatcaa
     6301 tcgcatcctg aggactgatc aaaccccact caagccagcg tctcgtaatc cgttctgccc
     6361 actttcccgc tcctatgcaa tggccaaacc gaggatgtcc tgcatccgga tatgcgacct
     6421 gcgcatttcc tagttgcttg cgatgccgta gcttttgaaa gcgccgacta tctacaatac
     6481 gccgtacccg tgttatatcg acggtcccag aaattgggga cgtgatttgc ttcaacgggc
     6541 gtcgcacaaa gttcaccgcc gaaacagaca ttcctgttcc ctccttactg gagaaatacc
     6601 acaaaggaac aagaatcaaa agggtagaat gactttactg tgcgatcaga cagctatcag
     6661 taccttcgag caagatggta gaggcaactt gaaggcctag gcgttctagc tcttctcgtt
     6721 ctttttcggt tagtagtaag attgccgtgt aggtgtaact catgaatgct gcaatcattt
     6781 cacctttttt ataaaatgca ggatgttcaa atccaaagaa gaactcttca tcataattgt
     6841 tcataaacaa gcccgccgca gacaaaactt ccttcacttt tccttgcaac ttgaaatagt
     6901 agtgctcata gcgagcatta ccttgtggaa tgtcattata ttttttattg gttacttctt
     6961 tgcgaataaa ttctttgctt acaaactcta attgacgacg gaaaatagga tctttctctg
     7021 taccaacttt tttccatggt agtggccata gttgccacca agtgctacgg gtcaaacgta
     7081 aggtatcgga atgcgcaatt aaaaaattaa gcagaacatc tgctttcgag gacctatatt
     7141 tgagggttac tatatactcg cgacggaccg actcaacttt cttcatatgg aaattatacc
     7201 aaatgaatca gatattacgc tatgtactaa attttcttat ttcgtctcgt attctacttt
     7261 tatccgatcg tgcagtatga agaaagaata actcggcatc taaaccctcc tgctctataa
     7321 attgagcaag cttatacata gtccagttac agaaactttt agtccccttg gtagaaatcg
     7381 gtattccggt gcgaggaatc gataaagcgt acgaggaagg acctgtttta tctatagcct
     7441 taccatgaaa ttgattcaaa gcaatcgatt catactgagt tacgtgggcg ttaccaatac
     7501 ctaaaatata acggggatgc acagatcgta tacgttcttt aaatcgttcg aaatcagact
     7561 ttaaatttcc aaatataaac acgtcactct ctaaatcctc tataaaggat actgtacgaa
     7621 agacataaac catgaatcgc tcattacctg acatatagtt caaagattaa ggagaaaaga
     7681 tatcctcgat acgcaaagta acttttttta tatctaacgg atcctggagg tacacccata
     7741 aaatttgcat cgaggctgat ccgcgaaggg ttactcgtaa gattgctgga ccttcttgta
     7801 acacttttac cccaggactg cattttaccc gcttgattat acccagtacc acctttttaa
     7861 cttccggtag gtctttcatg acagcaacga gcttttcagc aggggcaatc atactcgaat
     7921 gactgtgcga cactttattg ccgcgtaata atcctttttt ggctcccatt acttcttccc
     7981 catacgtgtg agtcgatacc cgacccagaa tatgacggct cctaacgtat tggccaccag
     8041 gtcccaggaa gtgtctgtca ggtgcaagtc aacgagcttg agttgcacaa taagaagttc
     8101 aaataactca ttagcaacac caaacgcact caccaaagca aaaagggtaa taacttcaaa
     8161 ccaaatatta aactttaaac caagagtagt tttaatatct agccagagaa aaccacaaaa
     8221 caacccacct cccacgacgt gcgtcatgat agatgtgtta cgtccttcaa taagtggaga
     8281 tggaagatac cacgaaataa aatagagaaa acaggcaaca agaagaagtc cagatggacg
     8341 tacttgctta cccgtaaggt atgctagaaa aggaggaaag agaaaatata tgagaagagg
     8401 aaccacaatc gaaataataa gaaaaatcat accgaaagta taccagcccc aaaccaaaac
     8461 actagtcaat taaatttaag gcttttaaac gagggagtat ttcatgggcg aagattgaac
     8521 caagtttcat tcctctagga ggcgcggaag ttatccaggc gagttcttcg acctcagagg
     8581 atggagttgg ttcgctttcc cactgcgtaa ccacaaatac cttgagatga accattcttc
     8641 cagggtgatt aactgcttca ctcgtaaact ctccaaaaaa ctccaggtcg ctttcctgca
     8701 cctttatcgt aaactcttca tgcagctcac ggatcaaaca tgtttccggc gtttcgcctt
     8761 cttcgatgct tcccccagga gaaataaaaa attctttccc tatcgaccgc tcgacaagga
     8821 gtttacggtt ttgaataatg attccggccg ctttatgaat atcgttcatc atgtggtaag
     8881 agctttcgat aaagtaggaa ccattgaaaa gtcagttatc tctgtggggt tatgagtaag
     8941 aagcgaaaat ccttgctcgt tataccgtgg cacgatatgc cagtgaacat gcttggcttc
     9001 gtctaaatac attaggtacg ctttttcaac cttatacgct tgacgcaagg cgttacgaac
     9061 attatcaaca actgtcataa gatggcgata ttcgttcggt ccgagcaagt gtaaatcaga
     9121 tacaggctct ttccatacaa cgagcgagtg accagccgta aacggaaagc ttgccaaatg
     9181 agcaatcacc tttttatctt cgtaaaagat agatccagta gcaggaggaa gtaacatatc
     9241 cataactata tggtatcaga aaaaaaacac ttgcgagaat aaattcctac tccacaactt
     9301 acttctttga aataggctct gggtaggggg tagttgccgc tggcaacttt atgtttcctg
     9361 gcttccccaa gccaaatgca gagtaacgct gcattcccgt aatacgctta ctcttatact
     9421 gcggttaacg acacaccaca atgcatctca
//
LOCUS       DASWYB010000035         9418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_276812, whole genome shotgun sequence.
ACCESSION   DASWYB010000035 DASWYB010000000
VERSION     DASWYB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276812"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VGE59_01970"
     CDS             complement(<1..457)
                     /locus_tag="VGE59_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY1074449.1"
                     /translation="MGSNEVIDLFSQKETFLLLIHHCPDGDAIASSLALGMALRFLGK
                     QVDIVCADPIPQAFRFLPTVHKVKHDFLSGDYEVIVTLDCGDSRRTGFSERIKELVRK
                     NNKLLVNIDHHPKNDLHSLATHNIVDYSAPSTTYIVYQIIKSLEVPIDHK"
     gene            complement(460..912)
                     /locus_tag="VGE59_01975"
     CDS             complement(460..912)
                     /locus_tag="VGE59_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074450.1"
                     /translation="MVVQRIQGNTIGLKLANALVNIKHEGGIRIGDRETEGPGEYDIA
                     GVGAHVLDSTAILFAEGIRIGIVWSVTADAKFEDDVNIDILIPFLTDVKQVNAFIKEQ
                     DPRVVVIPDEAIADEIAKQDGITVAKEANYKITSSTLPVEDRVFITLS"
     gene            complement(994..2064)
                     /locus_tag="VGE59_01980"
     CDS             complement(994..2064)
                     /locus_tag="VGE59_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY1074451.1"
                     /translation="MAKSQTIQTMEELVTVAGAELIPFRQGEVIEATVTQASKAKIMV
                     DIAGLALGFIPEREFSYEANDLQVGDKVLAYVLSVENDEGYVVLSMKRAEKEQTWRML
                     EEKVGAGDFLTVKVVQANKGGLLVRFGDVEGFIPVSQLSSQTLGRIPDRDKLTARLNE
                     LIGQALKVKAITSDKAANRLIFSEKAVANVETEAKIKSLKPGDTVKGKITGIVDFGIF
                     MDIGGVEGLVHISEVAWERIDDLKSKFAVGDELDVQIIEIDGNRVSLSVKRLLPDPWA
                     EAVKAYNVGQVVTGEVTRVTPFGAFVKFDAVLDGLVRVNEIVDAGQNPTSILEVGKTY
                     SFKILSIDPAARQIGLSYKQAQ"
     gene            complement(2129..2563)
                     /locus_tag="VGE59_01985"
     CDS             complement(2129..2563)
                     /locus_tag="VGE59_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074452.1"
                     /translation="MSGVKQLISYLTVQSTPPMSTYVSIAFGIAGVFLLVGISIPLLQ
                     FLLQRRKQSLKHLWLWQRLANVFRIAGCVWLVLLLLRFEGLAPFTMRVWLVLSVVPFL
                     IWTVWLVSRYGKMVPQIVYEDTLKTRFEKYLPQQSSKRRKKR"
     gene            complement(2585..2659)
                     /locus_tag="VGE59_01990"
     tRNA            complement(2585..2659)
                     /locus_tag="VGE59_01990"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2625..2627),aa:Phe,seq:gaa)
     gene            complement(2661..2735)
                     /locus_tag="VGE59_01995"
     tRNA            complement(2661..2735)
                     /locus_tag="VGE59_01995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2701..2703),aa:Val,seq:tac)
     gene            complement(2785..2870)
                     /locus_tag="VGE59_02000"
     tRNA            complement(2785..2870)
                     /locus_tag="VGE59_02000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2835..2837),aa:Leu,seq:gag)
     gene            complement(2971..3519)
                     /locus_tag="VGE59_02005"
     CDS             complement(2971..3519)
                     /locus_tag="VGE59_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074453.1"
                     /translation="MNQFLDSVRVFMAKLRVDFPSVALPRFTTTFALLWAIALLGLLY
                     GHVFFASSQSRSVSLFLSAAGGIGALFVILLHDPWYRLKILFNQVVALVLLLAFLIVA
                     SVTYSITQLVANVSPSIQFAWMVGSVLLAYVSLRAVFGAFFNLRRMTDIVYPGMRSTA
                     ILLFLFFLNYLWWSWEVIRASL"
     gene            complement(3538..3623)
                     /locus_tag="VGE59_02010"
     tRNA            complement(3538..3623)
                     /locus_tag="VGE59_02010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3588..3590),aa:Leu,seq:cag)
     gene            complement(3634..4956)
                     /locus_tag="VGE59_02015"
     CDS             complement(3634..4956)
                     /locus_tag="VGE59_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1074454.1"
                     /translation="MQPYTRRPAAVASSFSLAERILAGVAVILLGVGVFGMVKNRNQA
                     TELRPVHGGTYTEGVIADSPTKVDRIVMRLTNSGLTYRAADGSIKPALAESWEVSQDG
                     KQYRFRLREGFSAESFLATIQASKTNWTGITTSAPEPNVIQFVLPESLQLFLASTTVP
                     LFPYGPYKVAKRDKSEVVLRANNDFVLGKPYLEKFVIKQYETTDQLASAAKSGQIDGT
                     ADLTDVENRSFVRKDVSLPRYYVLFFNMTKPLFKKVEDRQRIIDLKDGNPVTYNLVTT
                     QSGDASDLADILAQNAKLKNITLNVQKKSGAALQKEDLPKREFDLLLYGINYGVDRDY
                     YPFWHSSQATATGLNLSGVKDRDLDRLLESARHEVDGAKRAQLNQQIEDLLSRNALQK
                     VLQQETFHFWIAKSIYGVEYGTMIDEGSDRFNLVWQWHKKVKKVPINS"
     gene            complement(4963..5178)
                     /gene="secG"
                     /locus_tag="VGE59_02020"
     CDS             complement(4963..5178)
                     /gene="secG"
                     /locus_tag="VGE59_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY1074455.1"
                     /translation="MHYLLIVQVIIALLLITTILVQQQGTGLGGAFGGEGNVYRSKRG
                     VEKLLYYGTIILGIAFFGVAIALVVTQ"
     gene            complement(5202..5275)
                     /locus_tag="VGE59_02025"
     tRNA            complement(5202..5275)
                     /locus_tag="VGE59_02025"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5241..5243),aa:Cys,seq:gca)
     gene            complement(5385..5459)
                     /locus_tag="VGE59_02030"
     tRNA            complement(5385..5459)
                     /locus_tag="VGE59_02030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5425..5427),aa:Gly,seq:gcc)
     gene            5580..5843
                     /locus_tag="VGE59_02035"
     CDS             5580..5843
                     /locus_tag="VGE59_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074456.1"
                     /translation="MAYNRKIQTGDIRSLTFKTQLMTPESPILIPLTIKATKLPRYQL
                     KKELAHIILSCAPLLILLVIATYFEETSHWVIPLAERVMKLGA"
     gene            5845..7359
                     /locus_tag="VGE59_02040"
     CDS             5845..7359
                     /locus_tag="VGE59_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY1074457.1"
                     /translation="MAIAHITTIGRLGMRCFPITVEVDVSHGLPAFNIVGLPDTIVQE
                     SKERIRSAIKNSGATFPLLRITVNLAPADIKKEGVGFDLPIALGILAASDQIPLPSAD
                     TYFYGELGLQGELKSTRGTLAFLIHAKNATRAFISSANQFEGGLVAERTPVYVADSLQ
                     TLVETLKGEQPETRITPHLPDSSPSFATDFADIHGQQAAKRALEIAAAGHHNILLSGP
                     PGAGKTLLSRAIPSIMPPLTHDEMLETTQIYSVAGLLSDEHPIIHSRPFRSPHHSASM
                     VAIIGGGNPPRPGEITLAHNGVLFLDEFAELPRMVIEALRQPLEDKVVTVARAGFSAE
                     FPAHCLLVAAVNPCPCGFQGDPKHHCTCTPQQLIMYKKKLSGPILDRIDLHLTVPAVE
                     VSDLTKTVTAESSQAVRDRVRSARNRQIERYKAIGLSANSQLTNKHIPHFCNQTPEAQ
                     GLIERAIDKLNLSARSYHRLLKIALTIADLEGSDTIQASHIAEAIQYRPQSSHH"
     gene            complement(7361..8284)
                     /locus_tag="VGE59_02045"
     CDS             complement(7361..8284)
                     /locus_tag="VGE59_02045"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY1074458.1"
                     /translation="MNEKLLLSIGGATGSGKTALAIRLAKKYPQLVILSADSRQIYRH
                     LDIGSAKVGAAGTSDILTGRPEPVWYSEGVPQFLIDIAEPNTNYSLADYQQEAYRLIL
                     SAWQRGYVPFLVGGTGLYIQAVVEGFVIGGEPDLVLRSELEHLSVDALQQRLEEVGGA
                     VVETDRLNKRRLIRAIERASGNVPEMKNKPLTTNQHTFVLERSWEEQRDLAPDMVQER
                     LDLGLIDEVRHLLVQGVNKDWLQTMGLSYRLVLRMLEGEFTEMALKENMIHEFRQLMR
                     RQRTWFRRMKQAHLMTAQEIEKEVTQILMTH"
     gene            8337..8975
                     /locus_tag="VGE59_02050"
     CDS             8337..8975
                     /locus_tag="VGE59_02050"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY1074459.1"
                     /translation="MAMTNLYFDIETIPAGEERIPFLKRLHEEKLAKDKELGTNNTPQ
                     DFELFYRKTALNAAYGRVFCIAMIKERDGIEIGKTTLCEDEKEMLRKFWGICRDVNCF
                     VGHGIRFFDIKFLMQRSIILGIPHTAIPLKKFSDYPVYDIMDQWMSYDGTISLHELAH
                     ALSIPSPKEGGIDGSQVYDFFLANKHEDICEYCMRDVETVRSVYQVLKKYRQ"
     gene            complement(9126..>9418)
                     /locus_tag="VGE59_02055"
     CDS             complement(9126..>9418)
                     /locus_tag="VGE59_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074460.1"
                     /translation="FCFKKEEPRRGLRGVISDKEGGSNPIRPMVSKLVVLLPTIANKD
                     KWVSWRSGRSDRVCDVGGYPRPCVLFSFLWCGRSLAVPGSNFFEGAEPIPAF"
BASE COUNT         2687 a         2434 c         1933 g         2364 t
ORIGIN      
        1 ttttatgatc aataggaact tctaaggact ttatgatttg gtaaacaata tacgtcgttg
       61 agggagcact gtagtctacg atattatgag tcgcaagcga gtgcaaatca tttttagggt
      121 gatgatcaat attaacaaga agtttgttat ttttacgcac caactctttt atgcgctcac
      181 tgaaacccgt acgacgactg tcaccgcaat caagtgttac aatcacttcg taatctccac
      241 tcaaaaaatc atgttttacc ttatggactg taggcaaaaa gcggaacgcc tgcggaattg
      301 gatcagcaca cacaatatca acttgcttgc ctaaaaatcg caaggccata ccgagcgcta
      361 agctagaagc aatagcatcg ccgtcagggc aatgatgtat caggagaaga aatgtttctt
      421 tttgtgaaaa aagatcaata acttcgtttg aacccatagc taggacagcg taataaagac
      481 tcgatcttca accggaagcg tcgagctcgt tattttgtaa ttagcctcct tggcaactgt
      541 gatcccatcc tgctttgcaa tttcgtcggc aatagcttca tcagggataa ccacgacacg
      601 tgggtcttgt tcctttataa aagcattaac ctgcttaacg tccgttaaaa aaggtataag
      661 gatatcaata tttacatcat cttcaaattt agcatcagcg gtgactgacc agacaatccc
      721 aatccgtatt ccttcggcaa aaaggatcgc tgtcgaatca agaacatgag cccctacacc
      781 agcaatatca tattcgcctg gcccttcagt ttcccgatct ccaattctga ttccaccctc
      841 atgtttaata ttgaccaaag cattggcgag tttaaggcca attgtattac cttgtatgcg
      901 ttgtactacc atagaaaacc tccagttgca ttctatcaca taaggacaag accaaaagaa
      961 agagagactg ccggagcagc ctctcttttc ttattactgg gcctgcttgt acgagagacc
     1021 aatctgtcga gcggctggat cgatcgagag aattttgaat gagtacgttt tacccacttc
     1081 aagaattgat gttgggtttt gccccgcatc aacaatctca tttactcgca ctaagccatc
     1141 gaggacggcg tcaaacttca cgaacgctcc aaaaggagta acgcgagtca cctcacccgt
     1201 cacgacttga ccaacattat aggccttcac tgcttctgcc caaggatcag gcaataaacg
     1261 cttgaccgag agagacacac gattgccatc gatttcgatg atttgaacat cgagctcatc
     1321 gccaacagcg aattttgact tcaaatcgtc aattcgctcc caggcaactt ctgagatatg
     1381 gaccaaacct tcaacgccgc caatatccat aaagatacca aagtcgacga tacccgtgat
     1441 tttaccctta actgtatcac caggcttaag cgatttaatc ttcgcttcag tttcgacatt
     1501 tgcaactgct ttttctgaga aaatcagacg gtttgcagct ttatctgaag taattgcttt
     1561 taccttcaaa gcttgaccga tcaactcatt caaacgagca gtaagcttat cacgatcagg
     1621 gatacggccg agagtctggc tcgagagctg agacacaggg atgaatcctt ctacgtcgcc
     1681 aaaacggaca agtaatccgc ccttgttagc ttgcactact ttaaccgtta agaagtcacc
     1741 agcaccaact ttttcttcga gcatgcgcca ggtttgttct ttttcggcac gtttcatcga
     1801 gagtacaaca tatccttcgt cgttttcgac agaaaggaca tatgccagaa ccttgtcacc
     1861 tacctgaaga tcattcgctt cgtatgaaaa ttcacgctca ggaatgaaac caagggcaag
     1921 gccagcgata tcgaccataa ttttagcctt agaggcttgg gtgacggtcg cttcgatcac
     1981 ttcaccctga cgaaatggaa taagttcggc accggcaacg gtaacgagtt cttccattgt
     2041 ctgaattgtt tgggacttag ccataatact cctcaaaaga acctaaagag gttctcgtcc
     2101 cgcgcctttt ggcgtcggac gaaaatccct atctcttttt tcgcctctta gacgattgct
     2161 ggggcagata tttttcaaac cgcgtcttta acgtatcttc atacactatt tgtggcacca
     2221 tttttccgta gcgagaaaca agccacaccg tccatataag aaacggcaca acggatagca
     2281 cgagccatac ccgcatcgta aaaggcgcta atccctcaaa acgtaacagt aagagaacca
     2341 gccacacaca accagcgata cgaaaaacat tcgcgagccg ctgccacagc cacagatgct
     2401 ttagcgattg ttttcggcgt tgtaaaagaa actgcagcaa gggaatcgaa atacccacca
     2461 agagaaaaac acctgcaata ccgaatgcaa tcgagacgta tgtcgacatg ggtggtgtcg
     2521 actgcaccgt cagatagctt ataagttgct tcactccaga catttcttgc aactaccttt
     2581 ctcatggtgc cgcgagtcag aatcgaactg acgacacgag gctcttcagg cccctgctct
     2641 gccactgagc taccgcggca tggtgggcga tgagggattc gaacccccga ccttctcggt
     2701 gtaaacgagc tgctctacca ctgagctaat cgcccgatat cgtaacatat gggcactctt
     2761 ccggattgaa accatcagag tacatggtgc cgcggagagg actcgaacct ccacgccctt
     2821 gcggacacta gcccctcaag ctagcctgtc taccatttca gcaccgcggc atgtataccc
     2881 atgaattatt cagattaaca gatcaatttt aacataccta aataggtctg tcgagattat
     2941 ttaacataag caggaacaaa aatataactc ttagagtgac gcgcgaataa cttcccaact
     3001 ccaccataag taattgagaa aaaagagaaa aagcaaaata gccgtactgc gcataccagg
     3061 ataaacaata tcagtcatac gtcggagatt aaagaatgct ccaaaaaccg ctcgaagcga
     3121 aacataagcg agaagaacag agccaaccat ccatgcaaac tgaatcgacg gagaaacatt
     3181 ggccacaagt tgagtaatgg agtacgtaac tgaagcgacg atcaaaaagg ccaggagaag
     3241 cacgagcgct accacctgat taaagagaat cttaagacga taccaggggt cgtggagcaa
     3301 aattacaaac aatgcaccaa tcccccccgc cgcacttaaa aaaagtgaaa ctgaacgtga
     3361 ttgagaagag gcaaaaaaga catggccata aagcaacccc agtagagcaa tcgcccacaa
     3421 taatgcgaat gttgttgtaa aacgaggaag tgccacagag ggaaagtcaa ctcggagttt
     3481 tgccataaaa acgcgtacgg agtcaagaaa ttggttcata tgtgttcctt acagaaatgg
     3541 tgccgaagag aggactcgaa cctccacgcc cttgcggaca caacgctctg aacgttgcgc
     3601 gtctgccatt tcgccacttc ggcttactat ctattatgaa ttaatgggga ctttctttac
     3661 cttcttgtgc cactgccaca ctaagttaaa tcggtcactg ccttcgtcga tcattgtacc
     3721 atattccacc ccgtaaatcg attttgcaat ccaaaaatga aaggtctctt gctgaagcac
     3781 tttttgtaac gcattgcggg aaagcaagtc ctctatctgc tgattcagct gcgcccgctt
     3841 ggcgccatca acttcatgtc gcgcactttc taacagcctg tctaaatcac gatctttcac
     3901 tcccgataaa ttaagtcccg tggccgtagc ttggctggaa tgccaaaacg ggtaataatc
     3961 acggtcaacg ccatagttaa tgccatacaa cagaagatca aactcacgtt ttggcaagtc
     4021 ttctttttgg agggctgcgc cactcttctt ttgaacatta agtgtaatat ttttcagctt
     4081 cgcattctga gctaaaatat ctgctaagtc acttgcatca cccgattgcg tcgtcacgag
     4141 attatacgta acggggttcc catccttcag atcaattatc cgttgcctat cttcgacttt
     4201 tttaaagagc ggtttcgtca tattaaaaaa caagacatag tagcgaggta aactcacgtc
     4261 ctttcttaca aaggagcgat tttccacatc cgtaagatca gccgtcccat caatttgacc
     4321 cgacttcgca gcgctcgcta actgatctgt tgtttcatac tgtttgatca caaacttctc
     4381 aagatatggt ttacccagaa caaaatcatt gtttgcccgt agcacaactt ctgatttgtc
     4441 acgtttagca actttgtaag ggccataggg aaaaagagga acggttgtgc tggctaaaaa
     4501 gagttggagc gattcaggta agacaaattg aataacgttc ggttccggag cagaggtcgt
     4561 tatgccagtc cagttagttt tacttgcttg aattgtggct aaaaaagact cagcactgaa
     4621 gccttcccgt aaacggaagc ggtactgctt accatcctga cttacttccc acgactcagc
     4681 gagcgctggt ttaatcgaac catcagctgc ccggtacgtg agcccgctat tggtaaggcg
     4741 cattacaatt ctatcgactt tcgttggact gtcagcaatg accccttccg tgtaggttcc
     4801 gccatggaca gggcgcaatt cagtcgcttg gttacgattt tttaccatac caaaaacacc
     4861 aacaccgagg agtataaccg caacaccagc gagaatacgc tcagccaagc taaatgatga
     4921 agcaacagct gccggccgtc tcgtgtaagg ttgcataacc gtttactgag tcaccaccag
     4981 agcaatagca accccgaaaa aagcaatccc caaaataatt gtgccgtaat aaagaagctt
     5041 ctccacaccg cgcttactgc gatagacatt tccctctccc ccaaaagccc cgcccaaccc
     5101 tgtgccttgt tgctggacga gaatagtcgt aatcaaaaga agagcaataa taacttgaac
     5161 aattaaaaga taatgcatcg taatccttta tctaaaatta ttggaggcgt gacccggaat
     5221 tgaaccgggg tgagcggttt tgcagaccgc cgcctaacca ctcggccatc acgccatatc
     5281 cctcttatac agaagacgga tggcgaaatc cagactgcgg tggcattccg tctttcgtca
     5341 tccgactaaa gagcctctct gtctttttta aaaacagggt gacatggagc gagagagggg
     5401 actcgaaccc ccgacctctt ccttggcaag gaagcgttct accactgaac tactctcgca
     5461 agttcgtcaa taatttacca tattcgcccc cttgtgtaaa gaacaggaaa cccgtagtat
     5521 ttcaatcaga atgaagttag ttctttatta ataattttaa gatttagaga aaggaagcaa
     5581 tggcttataa tcgaaaaatt caaaccggtg atatccgttc cctcacgttt aaaactcaac
     5641 ttatgactcc tgagtcgccg atactcatcc ctctaactat caaagcaacg aaattaccac
     5701 gttatcagct caaaaaagaa cttgctcata ttattttgag ctgcgctccc cttctaattt
     5761 tactcgttat tgccacttac ttcgaagaga cctcacattg ggtcatccct ctggccgagc
     5821 gagtgatgaa actcggagcc tagtatggca attgctcaca tcaccacgat cggacgacta
     5881 ggaatgcgct gtttccctat cacggtcgaa gtcgatgtga gccatggact tcctgcattt
     5941 aacattgtcg ggttacccga caccattgtt caagaatcaa aggaacgcat tcggtcagct
     6001 attaaaaata gtggcgctac atttccattg ctacggatta ccgttaactt agctcctgca
     6061 gatataaaaa aagaaggcgt aggctttgac ctaccaattg cacttggcat tcttgctgcc
     6121 agcgatcaga ttccactccc ttctgccgac acttactttt acggcgagct tggccttcag
     6181 ggcgaattaa aatcaacacg cgggacattg gcgtttctta ttcatgcgaa aaatgcgact
     6241 cgagcattta tctccagcgc aaaccaattt gaaggagggc tcgttgcgga acgaacgcca
     6301 gtctacgtgg ctgactcgct tcagacactt gtcgaaacct taaaaggaga acaacccgaa
     6361 actcgcatca ctcctcacct ccccgactca tctccttctt ttgccactga ttttgctgat
     6421 attcacggcc agcaggccgc aaaacgagca cttgagattg ctgcggctgg ccaccataat
     6481 attcttcttt ccggccctcc gggagcaggg aaaacgttac tctcgcgagc tatcccgagt
     6541 atcatgcccc ctctcacgca cgatgaaatg ctcgaaacaa cacaaatata cagcgtcgct
     6601 ggcctcttaa gtgatgaaca cccgattatc cacagtcgtc ccttccgatc cccgcaccat
     6661 agcgccagca tggtagcaat tataggtggg ggcaacccac cacggccagg cgagattacc
     6721 ttagcgcata acggcgtgct ttttctcgat gaatttgcag agttacctcg gatggtcatc
     6781 gaagcattac gtcaaccgct cgaagataaa gtcgttacgg tggcgagagc aggattcagt
     6841 gcagaatttc cggcacactg tctcttggta gcagcagtta atccgtgccc atgtggattt
     6901 cagggagacc ctaagcatca ctgtacctgc actccccagc aactcattat gtataagaag
     6961 aaattatccg gccccattct cgaccgaata gaccttcact tgacggttcc agcagtggag
     7021 gttagcgacc tcacaaaaac cgttaccgct gaatccagcc aagcggttcg ggatcgcgtt
     7081 cgatctgccc gaaacaggca gatcgaacga tacaaagcca ttggactcag tgccaacagt
     7141 cagctcacga acaaacatat tccacatttt tgcaatcaaa ccccggaagc ccaaggcctt
     7201 atcgaacggg caatagataa gctcaacctt tctgcacgaa gctaccaccg tttgcttaaa
     7261 atcgctctca ctatcgcaga cctagaagga agcgacacta ttcaagcaag tcacattgcc
     7321 gaagcaattc aataccgccc tcaatcctct catcattaaa ttagtgagtc atcagaatct
     7381 gcgtaacttc tttttcaatt tcttgcgctg tcatcaaatg cgcctgtttc attcgtcgga
     7441 accaagttct ttggcgacgc atgagttggc gaaactcatg aatcatattc tcttttaagg
     7501 ccatttctgt aaattcgccc tccagcatac gaaggaccaa ccgatatgat aagcccattg
     7561 tctgtaacca atctttattt accccctgaa caagtaggtg acgaacttcg tctatcagcc
     7621 caaggtcaag acgctcctga accatatcag gcgctaaatc tcgttgctct tcccaggaac
     7681 gctcgagaac aaatgtatgt tgatttgtcg tcaaaggttt atttttcatc tcaggtacat
     7741 tccctgacgc acgctcaatc gcacggataa ggcgacgttt attcaaacgg tcagtttcta
     7801 ctaccgcccc tcccacttct tcaagtcgtt gttgcaacgc atcgacagac aaatgttcca
     7861 gttcgctccg aagcactaag tcaggctctc ccccgatgac aaatccctcc actaccgcct
     7921 gaatgtacaa ccccgtccct cctacgagga atggtacata acctctttgc caagccgaaa
     7981 gaataagacg atacgcttct tgctgatagt cggcgagaga atagttcgta ttgggttcgg
     8041 cgatgtcgat caaaaattgg gggacgcctt ccgaatacca cactggttcc ggacgaccag
     8101 tcaaaatatc acttgtccca gctgcaccaa cttttgcact ccctatatct aaatggcgat
     8161 atatttgacg cgaatctgcc gatagaataa cgagctgcgg gtattttttc gccaacctaa
     8221 tagcgagtgc agtcttaccg ctccccgtcg cgccaccgat tgaaagtaga agtttttcat
     8281 tcataaccct actattatac taaacgtttg cctttctggc tccttcagag tagcatatgg
     8341 ccatgaccaa cctctatttt gatatcgaaa caatccccgc cggcgaagaa cgtatccctt
     8401 ttttaaagcg gctccacgaa gagaaattag ccaaagacaa agagcttggc acaaataata
     8461 cccctcaaga tttcgagctc ttttatcgca aaaccgccct caatgctgcc tacggcagag
     8521 tcttttgtat tgccatgatt aaagagcggg acggtatcga aattggcaaa acaaccctct
     8581 gtgaagacga gaaagaaatg ctccgtaagt tttggggaat ctgtcgcgac gtgaactgct
     8641 ttgtcggaca cggcattcgt ttcttcgaca ttaagttttt aatgcaacga tcaattattt
     8701 taggcattcc tcacacggca attcctctta aaaaattctc tgattatcct gtctacgaca
     8761 ttatggatca gtggatgagc tatgacggaa ccattagtct ccatgaattg gctcacgccc
     8821 tctctattcc ttcccctaaa gaaggtggca ttgacggctc ccaggtctac gatttctttc
     8881 tcgccaacaa acacgaagat atttgcgaat actgcatgcg cgacgtagaa acggtacgca
     8941 gcgtatatca agttttaaaa aaatatcgcc aatagggttg caaaaacgcg ataaatgtga
     9001 tatatattaa tcagttagga tagtggaact cggaggcctc catgcaaggt actcaggaac
     9061 cagaattgtc tcttccttta aaagcgatca tctactccgg tgatcgcgta ccaataggag
     9121 atgagtcaaa aggcggggat tggctccgca ccctcgaaga agttgcttcc tggcacggcc
     9181 aaactacggc cgcaccaaag aaaagaaaaa agaacacaag gccgaggata ccccccaacg
     9241 tcacacaccc ggtccgaacg tccgctcctc caactgaccc acttgtcctt gttcgctata
     9301 gtaggcagga ggacgacgag cttgctaacc attggacgga tcggatttga tcctccctct
     9361 ttatctgaga tcacgccgcg cagtcctctg cgcggctcct cttttttaaa acagaaaa
//
LOCUS       DASWYB010000036        21215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_438754, whole genome shotgun sequence.
ACCESSION   DASWYB010000036 DASWYB010000000
VERSION     DASWYB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21215
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438754"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VGE59_02060"
     CDS             complement(<1..1114)
                     /locus_tag="VGE59_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074461.1"
                     /translation="MSYEKLFQYAFEYGCGTPYEEVLQSELFQCELARRLSVYERHPL
                     GSRFGGVLNAGHFLATKKGHAISFDRRLPEGPPVDYSPEMLREQALIRVLGVSLIQAA
                     QECLSPEMRALAAGWEKADVTDRISLAAEFYNLAVAAKESPEQNLSDTPYGWLARFDK
                     PYPPQKVLPKQFGLYGQSGVMPCCLGFSLLLLAWTKYVGTKAYLTNTLTFNDHVIDAS
                     IERVSRRILGNIKHRGLYVKARNRRLFEQAVEFAHAKQRHVYDFHPAILLDLGEGYQL
                     YLDPYMGPFGFLKKGRSLGGATGVLSATEPVLPGMVAIATDGGFLEDKLRHEEKEVAS
                     LLKASYRLQQRVRRSFGTWDEVLAVLNESPEVEMLLT"
     gene            complement(1764..2234)
                     /locus_tag="VGE59_02065"
     CDS             complement(1764..2234)
                     /locus_tag="VGE59_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY1074462.1"
                     /translation="MLGNDSHLPVVHGYDPEGEYRNREWYRQMQLLEKARAFVRSTEG
                     KYGIGPFRFGLDAIIGLIPCGVGDFLTTVAAFYMVMIGISLQLPWWKVLMMVFHILAD
                     GILGTLQIIPFSGDIIDVFYKANRANLSIIEKHLIRRWTVEGGPQLPSPNSSYR"
     gene            2502..3245
                     /locus_tag="VGE59_02070"
     CDS             2502..3245
                     /locus_tag="VGE59_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074463.1"
                     /translation="MSKTRNIEKEPVAQTQTNQAEIRTKESKRYLVTPQGTELPLLQL
                     KGKDYLQVAHRLVWMREEHPEWQIITEPVEINVDKKYAIFRSSILNESGRVMATATKA
                     ESIAGFADYIEKAETGATGRAAALCGYGTQFAPELYEGERLADSPVDVPKKPVVNPAA
                     FTRQPSDIPTLLNRAKLRNATESCAACQSKNLTIGLTKPDNARNPSRPYWKCLDCPPE
                     KSFSHWVAETIVDEAKASNAPANLNKMPF"
     gene            3341..4600
                     /locus_tag="VGE59_02075"
     CDS             3341..4600
                     /locus_tag="VGE59_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY1074464.1"
                     /translation="MRDRQVFDENTLKNGIKTYTYKEDVPFTIVHLRIPVGSSHSTGD
                     IIPGSFHFLEHLLMDRSETYSNFNEFNRLVGLKGGHVSAATGPFETKFTLSIPTKHFP
                     SLSKGLLDQVFKPLFLEEDIALERGIIRNERTMKERWFPGSSEKGQYIATQWLNDCPL
                     TLRQRLGNDEDLARMTPESLRKVHRYYSDPRMKVVIGGSGYLDPFFEELAALSTQEHS
                     LTEHYKPVSWANREYHEKEFRDVSRHELYYGGISTPKPDINALGAIPFILNYLTNSIH
                     GPLYRWLREEKGWVYNLGSSFYSDRYSADWTLTFPLGDKEQVETVRKELWERVRSALN
                     DQDAINADIDRQLDYAKAYSFQRVSDILGFALNSLTIHERIIPESEMFAFAEKLRDRA
                     FLVKIFEEHFAQEHMGTFLATPLTVPV"
     gene            4668..4910
                     /locus_tag="VGE59_02080"
     CDS             4668..4910
                     /locus_tag="VGE59_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074465.1"
                     /translation="MDNTSNDPVQDLIDEGMERFGEGGPDGNIDDAIRRGEDQVEQDA
                     DPADGELESFRSDLGQEEPGTLEPGHKKDPTESDWL"
     gene            complement(4907..5350)
                     /locus_tag="VGE59_02085"
     CDS             complement(4907..5350)
                     /locus_tag="VGE59_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074466.1"
                     /translation="MAEYPRDSQELFSDSKSDTVESFDGQITVDQEWLGETQAEDYED
                     GKYDLTPEEEIERERYSAELWEDPSRAGADKSALFPDHLLTPEEQIERDLLLGEEYRG
                     SWGLGTASGLGFPANEDELESDERTEQNNTLHQMYRREQGLDDDI"
     gene            complement(5493..6329)
                     /locus_tag="VGE59_02090"
     CDS             complement(5493..6329)
                     /locus_tag="VGE59_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074467.1"
                     /translation="MKIIAGLIASLLVTLVFSVCLSLVMSRTILHGDYLSKELKSAQV
                     YPKLATAAGVLVAHQSEGQITPEAVEALIHKHLPEDIFQARAGTWLTELEKHMKENAA
                     APAFSLPELTAMAHDLGIDLPKNTIPQSFSLPAQTDEKITQNYQKVARTQQWLYIVAG
                     GLFLLLFLISVTTRSYGSLVYLFFASAALQGLMFAALRFGPNYLVAFIPKGEPGMEPI
                     IESLASVFYKVLSDMAIQFAIIAIVFLVVGFVILGLSLVSHARQENEKRATHRRTSSD
                     EA"
     gene            6461..7099
                     /locus_tag="VGE59_02095"
     CDS             6461..7099
                     /locus_tag="VGE59_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY1074468.1"
                     /translation="MVFWLAAMALLLMGMLTAPGVTLGLLAGFVYSSRFEHWVHAGPM
                     HHKSGGIAYERHVRDHHANRRAPVRYLAEKREQYRIGESAVPLIWLGHMIAIAGLWLI
                     TRQTSFWVSFTLIATVYFVLYEFLHHEMHQPHERWYHRTDLFQFLSELHRLHHYKAKE
                     NFNLVCPLWDWILGTLSTEELKPEASAPVGTPQPTGPRSVFSWFPEFAPKKS"
     gene            complement(7164..8975)
                     /locus_tag="VGE59_02100"
     CDS             complement(7164..8975)
                     /locus_tag="VGE59_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074469.1"
                     /translation="MKKPTPVNNFHQLLKNIRRVNAIIWPDHKRWIIMLFVIYGVISA
                     TPFLQSGSVGWLVNQLFAAIGQSIFNPSLTLAMIVLVLASTLPIFLLTYANYLEKMLY
                     LKMEEIFELLIARRRGEIDVASHEDPKKQDLFSKVDEDGVWRLQNFADRQLFIFQNIV
                     EVIIASVVIVLSEPWIFVLLFLCTIPELVVEARYGNDLWGMYGAKAETRRRFWDLRQH
                     FQRVPTLIELKVGQNTNRFVAMMGELFASFQSEQRKQERHKLIQQTFSHFIAQIAIAV
                     ALVWFVYEVLQGSMQVGTLTFVLASIAELRRSFSSLFTNLGKQYQDSLFVTDIFAVVD
                     MPPTLSAPENPIAVGAHTPEVVFENVAFAYPGTDRLVLKNVSFTIKAGEKIGLIGING
                     AGKTTLVKLLCRFYDPTSGRILIDGHDLKDLSPEEWYKRVGVIFQEYAHYNFPVKEAI
                     AIGDSGRPFSLQQVKDSAKRAGADSFITEWKDGYNQMLGKQFTDGVEPSIGQWQKLAL
                     ARMFYRNPNIVVLDEPTSSIDAEAESQIFEQLERLPEDRTVILITHRFATVRNVDKIV
                     VLKDGTISEMGSHKELVVNNKDYARLFYLQAKGYSSD"
     gene            complement(9081..9986)
                     /locus_tag="VGE59_02105"
     CDS             complement(9081..9986)
                     /locus_tag="VGE59_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY1074470.1"
                     /translation="MVSAILGDQTLEEAANIAAAQGYKYLEVMCWPQGSGEERRYAGV
                     CHINVEGFTPMMADDMHAVTQKHGIEICGLGYFANPLSDEGENRVVAAYVQDHMHKVL
                     KAASLMRVPFSTFVGRDQYAGLSANSTRFREFWPGFMSVAESLETEVCVENCPMHFSE
                     NEWPAGLNLAHTPAFWEWMFEEIPSRYFGLEYDPAHLVWQEIDPEFVMPKFLRRVKRA
                     HAKDARRDLRRRRLCGITASPLDYHTPCLPGEGDVDWDLFFEQLDHYEGPICVEVEDK
                     RYEGSLKSRIMALRRSREFLSHWYA"
     gene            complement(10207..10650)
                     /locus_tag="VGE59_02110"
     CDS             complement(10207..10650)
                     /locus_tag="VGE59_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074471.1"
                     /translation="MIKSLLGTVSKFGRRLGRSRPNSEIIVVSATIRMLPKSGRIILP
                     DVQHTIRTVLCRHLEQAQLVEGAQAPEYQVEMIGDEVPGRYQYYSWSFVVTRLSNGVV
                     MYASCHNDHYIPSVLERVLPELNRAIRTDRGSWYVETVRLPRLVR"
     gene            complement(10720..11388)
                     /locus_tag="VGE59_02115"
     CDS             complement(10720..11388)
                     /locus_tag="VGE59_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY1074472.1"
                     /translation="MRCVLHCHSLHSPDSSLPLQSIIDTCREYNINCVALTDHNEIAG
                     AIELQKMATDIKVIIGEEVTSKEGHIIGLFLKEKISAGMPIHDTIRAIRDQGGVVLLP
                     HPFDRIRPGSVGLKVLTQVAFEIDFLEIFNARCLLSSDNKKAAAFATEHRLRPYVGSD
                     AHFASEYTNAICDMPNFDTPEGFVKSLSQATFSTRPAGWVIHVRSNYVRHISKRITDP
                     KHRI"
     gene            11424..12311
                     /locus_tag="VGE59_02120"
     CDS             11424..12311
                     /locus_tag="VGE59_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEY1074473.1"
                     /translation="MEKRTKPIPLFLNTQAGAFKKTPEIEELTQLAQTYDIAIDVRPT
                     TSIEDLQQQIQTTIDQGEARIAVAGGDGTVAAAVQVLAYQNIELGIIPTGTANNFATA
                     LRLPMDIMSAMQVLKNGIPQKISLGKANGRYFTEAAGVGIFADALALYEGEHHKQFFK
                     GLLTLIRIFLNVRGYNLELEIDGQKYNEKSVMCTVANTFRIGYAFPVAPEAKLTDDQL
                     DIVLVGDLKRSELLPYFRAMRAQIHPTLPKVTTQKVKTVTIRARRKLNVHADDQIIGT
                     TPITIETHPGALTVLLDRL"
     gene            complement(12383..13834)
                     /locus_tag="VGE59_02125"
     CDS             complement(12383..13834)
                     /locus_tag="VGE59_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY1074474.1"
                     /translation="MSDVGTRLLTLGEAFDLTPDQNRALHLLHGNVALTGTLMMIDFD
                     PLFTHAYGTKVYDNTGRCYLDFKGGYGALPFGHHPRPILEAVVSAMARPNFLQASLSP
                     LGAVLKQNLAYISPGQALTRSFLCNSGAEAVEGAMKTARLYTGRRKIVYAERGFHGKT
                     FGALSVTGNKGYQDPFGPLVPDCVCIPFGSIAALKLVLRKKDVAAVILEPIQGEGGVR
                     ESPTGYLREVARLCRENGTLLIVDEIQTGLGRTGRMFACEEEGVVPDILCVAKALSGG
                     VIPIGAFLTTDEIWFACYGNLDTFALHTSTFGENSLACAAAIASIHEIVNQNLCEEAR
                     RKGRKLLTGLRSLQEKYPSVIHEVRGKGLLIGVELKHWAATGLDFLMKGTGSQLLGSL
                     VAGKLLVDHGIITVYTLNKPRVIRVEPPLTVTDEEIEYFLQSFEAVLAGYKGFPALMA
                     SKFGNGRRLNLAMRVMRGVMPVGQDEIERMLSR"
     gene            complement(13967..15514)
                     /locus_tag="VGE59_02130"
     CDS             complement(13967..15514)
                     /locus_tag="VGE59_02130"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY1074475.1"
                     /translation="MEKNLLALFDTESQADSAVIELSRRGYRTENMSVIASNSTRSNW
                     VDSDTPEDRVTEGVASGAALGGLAGLLAGAGVVPALAGILIGGPFAAALGLTGLAAAT
                     VSGAITGGMAGGLVGALSNFGLSEADANEFSEGVNRGGVVVALPVTEKTQDEVTQVLQ
                     DAGARKVNLAVANSNESSANSSISDSHGDNPFTAEQVNKQESFKAKDIIGKTIMSYSD
                     GKAVYKVYDIIHDAFNQKVLGFLVDESWILARGRHLPLSAIRSIGKDAVTVDTGDTVL
                     MAKDDPQVKRILDSGSDIRGHKIVTEDGEEVGKVTDFYFQGDTGVIDGYEVSRGLSSD
                     TYKGKAYLPAAEVAKIGEELIFVRRDIGDMLNEQQTGGVQGAVDKAKGKTQETIGAAR
                     EKVAQGHYSPEFMQATVAPAAETVKEKTMSAWEKIKEQISDMKEQSSQALEAKRIKAA
                     LGRPVTRVILDSEDNVILNTGDLITHESVNRAREHNILDALLDSVYKDKPEFTNNELR
                     ADDQDEV"
     gene            15687..16517
                     /locus_tag="VGE59_02135"
     CDS             15687..16517
                     /locus_tag="VGE59_02135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEY1074476.1"
                     /translation="MFDPKKRNHHIQYAQNFLNNPSLARYVVGLSNITSNDSVVEIGP
                     GKGMLTNILIKKCRHLTLVEKDRELALELRQRYAEAPTVKIIHADALQFRTEEANYKV
                     FSNPPFNILSQVIKKFVFAKNAPQEMYLFMQKEAQQRLSGKSGENQLSILIKSFYTLK
                     ELHKFKPSDFSPVPSIDVNLIGFYSKSGNPILPVDKQEYILFVQFGFQQQKATLEKNF
                     DKVFTHEQWKRLAKDLNFSLKSLPTDLKPDHWIGLFRFYKIGVTPEKQRIVQAFAPRK
                     "
     gene            complement(16568..17143)
                     /locus_tag="VGE59_02140"
     CDS             complement(16568..17143)
                     /locus_tag="VGE59_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074477.1"
                     /translation="MARIIHVREVTRDQVSKVAGFLVRLPDGSEREITVKEVSNPVSY
                     DADNVLEVTPEPFISLVTRTWPNRSVGYYSQRFVATRWKAGSSTTYTVGDEVFFLNEA
                     EVCSLTGHISVVCLGRIQMIDEQGSLYVGFIANPDTSPTRWFLKCRAEELMVVAKREP
                     LGTYGLKLEVKGLYGEAIVEIPLGEPPSAPR"
     gene            complement(17349..17942)
                     /locus_tag="VGE59_02145"
     CDS             complement(17349..17942)
                     /locus_tag="VGE59_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEY1074478.1"
                     /translation="MTTQRGILHQLGVWLRDQLVVPDDLPDGDTRLLAIIVIPFSLTN
                     GALGPKMTAIVDRAVELCNLYQVDNLVLTGGWESNGKKEGTLAAEAVYGRISPHIRII
                     VEDETLNTFAQARYLQDVVPKLEGEGRPVLILAEEMHMRRVLLLACRRLGRLTSVFHA
                     SAHSLGWDNDKWVLRHPLTFGVREVLLLALLALFRNL"
     gene            18089..18757
                     /locus_tag="VGE59_02150"
     CDS             18089..18757
                     /locus_tag="VGE59_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1074479.1"
                     /translation="MRILIIEDDRTLATSLQEQLSEVYKVDIAHTGARGQRQAEVTAY
                     DLILLDLDLPDIHGKDVCFELRKKGIKAPVLIVTGEADIEIKTSCLDAGADDYITKPF
                     HSQELFARIRALLRRNPDTALSNHLRVKDLELDPATRIATRQGKTIPLRRKEFDILEY
                     LMRNPNRVLTRDMILEYVWGDTSEPFTNSISVHIKHLRDKIDYPFEPKLIQTVHSLGY
                     KIQG"
     gene            18976..20376
                     /locus_tag="VGE59_02155"
     CDS             18976..20376
                     /locus_tag="VGE59_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1074480.1"
                     /translation="MKISLQRLDHVKKLLIAPKGASEDTRRREYILNLLLLGSIALSA
                     IALFSAFLNVLARSDGYRGESLLVLCIPMGFFLCLYALSRIRYPKIAASVFIATYYIL
                     ATYTLAKWGILLPQGILIYAVIIVMTGVLLSTNAAFFVTCLLSVTLLTLRVFEIQGTL
                     HPNLYWIHESGGIDDAIVFSATLFIILIVSWLSNREIYKSLERARKSEASLKEQRDSL
                     EIIVEERTQELRAQQREENIRLYRFAEFGQLTSGLLHDLTTPLTTLSLNLERLSNHED
                     SQTIKRAIDAARRMHLYIDAARKQLSPNVEITCFKPIEEIQEAQKILAHRSRKAHIKF
                     TCSMSKDIKLYGDPIKFNRLATNLIANAIDAYDTSPPAVQKNILLTLEKNDTAVIFKV
                     ADQGCGIQKKEQGNIFLPFYTTKSNQKGIGMGLAICKEIVEKDFHGSIEVSSSSETGT
                     TFTITIPITHIHEQNS"
     gene            20360..>21215
                     /locus_tag="VGE59_02160"
     CDS             20360..>21215
                     /locus_tag="VGE59_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY1074481.1"
                     /translation="MNKTAKTLGVSFLAASQGQHGGYLSHSSSDPSTFKDTSTYSTTF
                     ASALILDCLHNCPHTENIKQKLVSFLLSQKTPSGSFNYWDRNDPITQTLPYPDDLDDT
                     FCALAALHRANKKGTNGKTLAQAVTLLALTEIKEGGPYKTWLVPDNAAAVWHDVDPAV
                     NSNIAYFLSLQEVELVSLVELTENAIQSANYTSPYYPNEYPLFYFISRWYRGRLLTSL
                     ADAILSRQLSDGSWGSPLKTALALTSLLRLTLPVPKIHQAVSFLCKTQNSDGSWEAEG
                     FCYDPMIQG"
BASE COUNT         5898 a         5577 c         4565 g         5175 t
ORIGIN      
        1 ccgtcagaag catttcgact tcgggactct cattcagaac agcgaggact tcatcccatg
       61 tcccaaacga ccgtctcacc cgctgctgga ggcgatatga cgccttcaag agtgaggcaa
      121 cctccttttc ttcgtgacgc agcttgtcct cgaggaaacc gccatccgtt gctatagcaa
      181 ccataccagg gagaaccggt tccgtagccg acaagactcc tgtcgctccc cccaatgacc
      241 ttcctttctt caggaatcca aagggaccca tgtacggatc gagatacaac tgataaccct
      301 cacccagatc tagcaagatc gcaggatgga agtcatacac gtgtcgctgc ttggcgtgag
      361 caaactcgac ggcttgctca aagagccgtc gattgcgggc ttttacgtac agcccacgat
      421 gtttgatatt tcccagaatg cgtcggctga cgcgttctat cgaagcatca ataacgtgat
      481 cgttgaacgt gagggtattt gtgaggtacg cctttgtccc aacgtacttc gtccacgcaa
      541 gcaagagcag ggagaacccc aggcagcatg gcattacacc agactgacca tagaggccga
      601 actgcttagg caacaccttc tgaggaggat acggcttatc aaaccgtgcc agccagccat
      661 acggagtatc cgataaattt tgctctggag actctttggc cgccaccgca agattataaa
      721 actccgcagc cagactaata cgatccgtta cgtcggcttt ctcccatccc gcagcaagcg
      781 ccctcatttc tgggctcaga cattcttgag ctgcttgaat aagagacaca ccaagcactc
      841 ttataagcgc ctgttcacga agcatctctg gagaataatc aaccggcggc ccctcgggca
      901 accggcggtc gaaactaatc gcgtgtcctt ttttggttgc taggaagtga cccgcgttaa
      961 gaactccccc aaacctactt cctagaggat gacgctcata caccgacaac cgtcgcgcca
     1021 actcacattg gaaaagctct gactgcaaca cttcctcgta aggagtgcca cagccatact
     1081 caaaagcata ctgaaacagc ttctcgtagc tcatgacact cctcgtagaa cgatgacgat
     1141 ggaaagcttc ttcgaacagc gtaattctaa caagtatagt ccttaccgtc aacccataaa
     1201 agtatttaaa tttaaaaaca ccctccggag ttacatgtaa ctcaagaagg tgttttggaa
     1261 cctgccttcg tagtcttttc caggcgaaag gcgttcggct gagacgagac agatgcaacg
     1321 cgaggagaga tagattcctc tctcgacgag gggtgcgttt gtcgtagtcc agccgggcgg
     1381 cttgcagctg aaagggactg cgaaggcagg ttcatggtgc cccaaacatt caattatgta
     1441 aggtatcatt gacccatagt cgcacaaagt atgaggcatc tctcttttgg caaaacctag
     1501 gagacgaacg ccccaaatga gcggaaagaa tgtatcgatc aatgatgagt gatgttaagg
     1561 acagttctga tactatcgta tacggttcaa atcgtcttgg tgccgaataa caaagaatac
     1621 gataaaattt ttatattttt atagacccga acaaagacga aatcaactct cgaaacagag
     1681 tatttaccat tagcaacaaa acgcttgttc gaaaatcaag catgtagaaa tcaaaaggcg
     1741 ccgagttttt agctcggcgc ccctcaccga tacgatgagt taggggaagg gagttgagga
     1801 ccaccctcga ctgtccaccg acgaattagg tgtttctcaa taatactgag attcgcccga
     1861 ttcgccttgt aaaagacgtc gataatgtcc ccactgaagg ggataatctg cagtgtgccg
     1921 agtattccat cggcaagaat gtggaagacc atcatcagga ccttccacca aggaagctgc
     1981 agcgaaatcc caatcataac catatagaac gcagcaaccg ttgtgagaaa gtcaccgacc
     2041 ccacagggga tgagcccaat tatggcatcg agaccgaaac ggaagggacc gatgccgtac
     2101 ttaccctccg tcgaccggac gaaggcacga gccttttcga gcaactgcat ctgccggtac
     2161 cactcgcggt tccgatactc tccctcaggg tcatacccgt gaaccacggg taggtgggaa
     2221 tcattaccca acatttgatc ctctttaatc agaaacgata aatggagaat atactaaata
     2281 tggcattttg tcaatccgtg caagccatct atgacgcctg ctcgtttgtt gctatcccga
     2341 acaataaccg ataaagtgag aacgtagtat taaggaagaa aggaggtgca gcagtggata
     2401 taattgaatg gctgcggcgc ctgttaacgc cgccgcaggt tgctcccgaa agagttcgag
     2461 tcccctcacc gaagggacac actagaaaat aaaggaacat catgagtaag acacgtaaca
     2521 tagaaaaaga gcccgttgcc cagacacaaa ccaatcaggc agaaatccgc acgaaggaaa
     2581 gtaaacgcta tctcgttacc cctcaaggta ccgaactccc cttgctgcaa ctcaaaggta
     2641 aagactacct ccaagtcgcc catcggctgg tatggatgcg agaagagcat cctgagtggc
     2701 aaattatcac cgaacctgtc gaaattaacg ttgataaaaa gtacgccatc ttccgttcct
     2761 caatcctaaa cgagagcgga cgagttatgg ccacggccac gaaagctgag tcaatcgccg
     2821 gctttgccga ctacattgag aaggccgaaa caggcgccac gggccgtgct gccgccctct
     2881 gcggttacgg tacgcagttt gcccccgagc tgtacgaagg cgaacgtctt gcagactcac
     2941 ccgtcgacgt acctaaaaaa cctgtcgtta atccggctgc gtttacccgt cagccgagcg
     3001 acattcccac cctcctcaat cgcgccaagt tgcgtaacgc aaccgagtca tgcgctgcct
     3061 gtcagtccaa aaacctcact attggattaa caaagccaga caatgcgcgt aatcccagcc
     3121 gtccctactg gaaatgctta gactgtcctc cagaaaaaag cttttcccac tgggtagctg
     3181 aaaccattgt tgacgaagca aaagcctcga acgctcctgc caacctcaac aaaatgccgt
     3241 tctaacgaca tcaataaaga aaaacagccc cattgtggct gtttttctta ttatgctctg
     3301 tgctagagta gaaccacgac cgcaagccca aaggaaatct atgcgcgaca gacaagtctt
     3361 tgacgaaaac acactcaaaa atggcataaa gacatacacc tacaaagagg acgtcccctt
     3421 tacgattgtc catctgcgca ttccggttgg gtcgagtcat agcacaggcg atattattcc
     3481 gggttcgttt cactttttag aacacttgtt gatggaccgt tccgagacct actccaattt
     3541 taacgaattt aaccggctcg ttggcctcaa aggcggccat gtttcggccg caaccggacc
     3601 ttttgaaacc aaattcacac tttcaatccc gaccaaacat tttccttcgc tgagcaaagg
     3661 cttgctcgac caagttttta agcccctttt tcttgaggaa gacattgcct tagagcgagg
     3721 aataatccgt aacgagcgca ccatgaaaga gcgctggttt cctggatcct cggagaaagg
     3781 ccagtacatt gccactcagt ggctcaatga ctgtccgctc acactgcgtc aacgcctcgg
     3841 caacgatgaa gatctcgccc gcatgacacc agaatcactc cgcaaggttc atcgctatta
     3901 ctctgatccg cgtatgaaag ttgttattgg aggatcgggt tacttagacc ccttcttcga
     3961 agagctggca gctctttcaa cacaagaaca ctccctcacc gagcattata aacccgtcag
     4021 ctgggccaat cgtgagtatc acgaaaaaga gttccgcgat gtgagccgac acgaactata
     4081 ctacggtgga atttcaacac caaagccgga tattaacgcc ttaggcgcga ttccttttat
     4141 tttaaattac cttaccaact caattcacgg cccgttatat cgttggctcc gcgaagaaaa
     4201 agggtgggtt tacaacttag gttccagttt ctattccgac cgttactcgg cagactggac
     4261 actcaccttc ccattgggag acaaagagca ggttgagaca gttcggaaag agctgtggga
     4321 acgtgtacgc agtgccctca acgatcaaga cgcgattaat gccgacattg atcgtcagct
     4381 cgactatgcc aaagcttact ctttccaacg tgtgagcgat attctcggat ttgcccttaa
     4441 tagccttact atccacgagc ggattatccc cgaaagcgaa atgtttgcct ttgcggaaaa
     4501 gctgcgagat agagccttcc tggtaaaaat attcgaagaa catttcgccc aagaacacat
     4561 gggcaccttc ttagctactc ctcttaccgt gcccgtgtag catttgtatt ttttactaat
     4621 gtcgaaacta tggtaacgtc ttactattat gatacaagga gatgtctatg gataacacaa
     4681 gcaacgaccc agtccaagat ttaattgacg aaggcatgga acgatttggc gagggcggcc
     4741 ccgacggcaa tatcgatgac gccattcgcc ggggagaaga tcaagtagaa caagatgctg
     4801 accctgctga cggcgaacta gaatcatttc ggagtgacct aggccaagaa gaacccggca
     4861 ccctcgagcc tggacataaa aaagacccta ccgaatctga ctggctctaa atatcatcgt
     4921 cgagaccttg ctcacggcga tacatttgat gtaacgtatt gttttgctca gtacgttcgt
     4981 cggactcaag ctcgtcttcg ttggcaggaa atcccaggcc actcgcggta ccaagtcccc
     5041 agcttccccg atactcttca ccaagcagta agtcccgttc aatttgttct tcgggtgtaa
     5101 gcaagtggtc ggggaaaaga gcactcttat ccgcacccgc acggctagga tcctcccaga
     5161 gctctgcgct atatctctcg cgttcgattt cttcttcggg ggtcaaatcg tatttaccgt
     5221 cttcataatc ctcggcctgg gtctcgccaa gccactcttg atcaacggtt atttgtccat
     5281 caaaactttc aactgtatcc gacttactat cagaaaacag ttcttgtgag tcacgtgggt
     5341 actctgccat ctctcctcct ttaggcaatt catgcgccaa gagtacgtct cttcgctcac
     5401 gctattttac atacaaaaaa atgactccac acgagttaac attctaagca gataccccgt
     5461 aaaatagttt tttttaggat taatcctaaa ggttaggctt cgtctgacga cgtccgtcgg
     5521 tgcgttgctc gtttttcatt ttcttgtcga gcgtgagaaa ccaatgacaa tccaagaata
     5581 acaaatccaa caacgagaaa gactatggcg atgatggcaa actggatcgc catatcagat
     5641 agcactttat agaatactga agcaagcgac tcaataattg gttccattcc cggctcgcct
     5701 ttgggaataa aagctacgag ataattcgga ccaaaacgta aggcggcaaa catcagccct
     5761 tgcaaagcag cactggcaaa aaataggtac actaaactgc cgtacgacct ggttgtgacc
     5821 gatataagga aaaggagtaa aaataaaccg ccggcgacaa tgtaaagcca ctgctgagtt
     5881 cgtgctactt tttggtagtt ctgagtaatc ttctcgtcag tctgggctgg taaagaaaat
     5941 gactgaggaa tcgtattttt cggcagatca atccccaagt cgtgagccat tgcagtaagt
     6001 tcgggcaagg agaaggcagg agcagctgca ttttctttca tgtgtttttc gagctctgta
     6061 agccaggtac ccgctctggc ttggaaaata tcttcgggta aatgcttatg aatcagtgcc
     6121 tcgacagctt cgggtgtaat ttgaccttcc gactggtgag caaccagcac accggcggca
     6181 gtggcaagct tgggatagac ttgagccgat ttgagttctt tggaaagata gtccccgtgc
     6241 aaaatagtac gagacattac taagctgaga cagacgctaa atactaatgt cactaaaagc
     6301 gaggcaatca agccagcaat tattttcata cattcagcgt aaaccaaccc agcataaatg
     6361 taaaaccagc ctcttcatcc gttgaccaat acttcgaggt attagatagt aatagcgggt
     6421 aagcacttga gctacctggg tatgaaaaag gtatcgaacc atggttttct ggctggcggc
     6481 tatggctctg ctcttgatgg ggatgctaac cgctcctggt gtgacgctcg ggttactggc
     6541 tgggtttgtt tacagcagca gatttgagca ttgggtccac gcaggaccca tgcatcacaa
     6601 gtcgggaggc atcgcgtatg aacgacacgt ccgcgatcat cacgccaacc gacgcgcacc
     6661 agtacgctat ctagctgaga agagagaaca gtatcgtatt ggcgagtcgg ccgtcccgct
     6721 catctggctc ggccatatga ttgcaatagc aggattatgg ctgatcacga gacaaacatc
     6781 cttctgggtg agctttacgt tgatcgccac tgtttacttc gttctttatg aatttctgca
     6841 tcacgagatg caccaaccgc acgagaggtg gtatcatcga acggatcttt ttcagttcct
     6901 ttcggaacta caccgcctgc accattacaa ggcaaaagag aacttcaacc tagtctgtcc
     6961 tttgtgggac tggatactgg gaacattgag cacagaggaa ctaaaacctg aggcatccgc
     7021 gcccgtcgga acccctcagc caacgggtcc tcgatcggtt ttctcctggt tccctgagtt
     7081 cgctcccaaa aagtcataga ctggtgccgc aggactctcg tcgccaacac acttcccctg
     7141 cagcttgtct gcggggattt ttcttagtca gaggaatagc ctttggcctg aagataaaat
     7201 aaacgggcgt aatctttatt attcacgacg agttccttat gcgagcccat ttcgctaata
     7261 gtgccgtctt ttaacaccac gattttatcg acgtttcgta cggtagcaaa ccgatgcgta
     7321 ataagaataa ccgtccggtc ttcgggcaac cgttcgagct gttcgaaaat ttgggattct
     7381 gcctcggcat caattgatga agtcggttca tcgagaacaa cgatgtttgg gttacggtag
     7441 aacatgcgcg ctaatgctaa cttttgccac tggccaatcg acggctctac accatcggta
     7501 aactgttttc ccagcatttg gttataccca tctttccact ctgtaataaa cgaatcggca
     7561 cccgcccgtt tcgccgaatc tttcacttgc tggagagaaa acggtcgacc cgaatcccca
     7621 attgcaatcg cttctttcac gggaaaattg tagtgcgcat attcttggaa aataacacca
     7681 acgcgtttat accactcttc gggagagagg tctttcaaat cgtgtccatc gattaaaatt
     7741 cgacctgaag taggatcgta aaatcggcag agcagtttca ctaaggttgt tttacccgca
     7801 ccattgattc caattaagcc aatcttttca ccggctttaa tggtaaaaga aacatttttc
     7861 aaaaccaacc gatcagtgcc cggataggca aacgcgacat tttcaaagac aacctctggc
     7921 gtatgggctc caacagcaat tggattctcg ggagctgaaa gagttggtgg catatcaacc
     7981 accgcaaata tatctgttac aaagagacta tcctgatatt gcttgcctaa gttggtaaac
     8041 agactcgaga atgatcggcg taactcggca atcgaggcca atacaaaggt gagggtacca
     8101 acttgcatcg aaccttggag tacttcatac acaaaccaga ctaaagcaac cgcaatcgca
     8161 atctgagcaa taaagtggct aaaggtttgc tggatcaatt tatgacgttc ctgcttgcgt
     8221 tgctccgact gaaacgaggc aaaaagctca cccatcatcg ccacaaaacg attcgtattt
     8281 tgaccgactt ttaattcaat aagggttgga actcgctgaa aatgctgccg taaatcccaa
     8341 aatcgacgac gcgtttcagc cttggcgccg tacatacccc aaagatcgtt accatatctc
     8401 gcttcgacga ctaattcagg gatggtacag agaaagagca gaacaaaaat ccacggctcg
     8461 gataacacaa tgactaccga ggcaattatg acttcaacaa tgttttgaaa aataaacagc
     8521 tgacgatccg caaagttttg tagccgccat acgccatctt catccacttt agaaaataag
     8581 tcctgttttt tgggatcttc gtgtgacgcc acatcaattt cgccacgccg acgagcgatc
     8641 agcaactcga aaatttcttc cattttcaga tagagcattt tctccagata gttcgcatag
     8701 gtcagtaaaa agatcggtag tgtcgaagct agcaccagta ctatcatcgc gagcgtaaga
     8761 gaaggattaa aaatactctg gccaattgct gcaaacaatt ggttcacaag ccacccgact
     8821 gatcccgatt gcaaaaatgg cgttgccgaa ataacaccgt aaattacaaa caacataata
     8881 atccagcgtt tatggtcagg ccaaataatc gcattcacgc gccgaatgtt ctttaatagt
     8941 tgatgaaagt tattgaccgg agtaggtttc ttcatactat tttatactat cactttatac
     9001 tcatacgaaa agtaaaagcc gtaggtggtg ccaaaatggc aacgcacacc cacggcttcg
     9061 gcggtcgaat cgtcggctcg ttacgcgtac cagtgagaca ggaactcgcg gcttcggcga
     9121 agcgccataa tacggctctt caggcttccc tcatagcgct tgtcttcaac ctcgacgcag
     9181 atcggcccct cataatggtc gagctgctcg aagaagagat cccaatccac atcaccttcc
     9241 ccaggcaggc agggtgtgtg gtagtcgagc ggagaagcgg taatcccgca caggcgacgc
     9301 cggcgcaagt cgcgtcgggc atccttggcg tgagcccgct taaccctacg caagaacttc
     9361 ggcatcacga actcgggatc aatctcctgc cacacgaggt gggcaggatc gtactccaac
     9421 ccgaagtacc tactcgggat ctcttcgaac atccactccc agaaggccgg cgtatgggcg
     9481 aggttgagac cggcaggcca ttcgttttcc gagaaatgca tggggcagtt ctcgacgcag
     9541 acctccgttt ccaggctctc ggccacgctc atgaaccccg gccagaactc cctaaatcgg
     9601 gtgctattgg cggacaagcc cgcatactgg tcacggccga cgaaggtact gaatggcacg
     9661 cgcatcaggg aggcagcctt caagaccttg tgcatgtggt cctggacgta tgccgcaaca
     9721 acgcgattct ccccctcatc ggaaagcgga ttggcaaagt aacccaagcc gcagatctca
     9781 ataccgtgct tctgcgttac cgcgtgcata tcgtcagcca tcatgggggt aaacccctcc
     9841 acgttgatat ggcaaacgcc ggcgtagcgc cgctcctcac ccgagccctg tggccagcac
     9901 atgacttcga ggtacttgta cccctgcgcg gcagcaatat tcgccgcctc ttcgagcgtt
     9961 tggtcaccga gaatcgcgct gaccaagccg agccccccct gtctctttgg taaagacact
    10021 gtcgtctcct cctagaaaaa gcagccagtc gctgcttcta aaagagagta tagatatata
    10081 cgaagaattt gtaaagtaca ttcgtatcgt tagaaagttg ctgagtataa aaaacgggct
    10141 gcgttctcca tagtggaata acgcagccgt cagaatctga aaatgtgtcg ccccttgccg
    10201 actcagttat cgcacgagac gcggcagacg aaccgtctcc acataccaac taccacgatc
    10261 cgtgcgaata gcgcggttca actcgggtaa taccctttcg agtacactcg gaatgtagtg
    10321 gtcattatga cagcttgcat acataaccac accattcgat agcctggtaa caacaaagct
    10381 ccagctataa tactggtatc gtcccggtac ttcgtcacca atcatctcaa cttggtattc
    10441 gggggcttga gcaccttcga ccagttgagc ctgttcgagg tgcctgcaaa gcaccgtacg
    10501 gattgtgtgt tgtacgtcgg gaagtatgat ccgtcccgat ttaggaagca ttcgaatcgt
    10561 cgccgaaaca acgatgatct cagaatttgg ccgagacctt cccaacctgc gcccaaactt
    10621 agaaaccgta cctaacaaag actttatcat gtacctcata cccccaagag aaccagcaca
    10681 agaactatac tataaaatac gaagagaatc aatgcactct cagatcctgt gtttgggatc
    10741 tgtaattctt ttacttatat gccggacgta atttgaacga acatgaatca cccacccagc
    10801 aggtctcgtg gagaatgtgg cttgagacaa gcttttaaca aatccttcag gcgtatcgaa
    10861 attgggcatg tcacatattg cattggtata ctcactcgca aaatgagcgt ccgacccaac
    10921 gtaaggccgt aacctgtgct cggtggcaaa ggcggcggct tttttattat cgcttgagag
    10981 caagcaacga gcattaaata tttccagaaa atcaatttcg aaagctacct gggtgagcac
    11041 ttttagccct actgatccag gacggatgcg atcaaaaggg tgaggcaaga gcacaactcc
    11101 gccctgatcg cgaattgccc gtatcgtatc gtgtattggc attccggcag aaatcttctc
    11161 ctttaaaaag agaccaataa tgtgtccttc ctttgaagtt acttcttcac caataatgac
    11221 ctttatatct gtggccattt tttgtaactc aattgcgcca gcgatttcgt tatgatcggt
    11281 gagagcgaca caattaatat tgtactcccg acaggtgtca ataatgctct gcaaaggcag
    11341 cgagctgtcg ggagaatgga gactatgaca atgcaataca caacgcatgt atgccactat
    11401 agccaaacaa ggatacactg actatggaaa aacgtaccaa acctatcccc ctctttctga
    11461 atacccaagc aggtgcattt aaaaagactc ctgagatcga agagctgact cagttggcac
    11521 agacatacga cattgccatc gatgtacgtc ccactacctc catagaagac ctacaacaac
    11581 agattcaaac aacaatcgac caaggagaag cccggattgc cgtagccggc ggagacggaa
    11641 cagttgccgc agccgtccaa gttttggcgt accaaaatat tgaactcggc attatcccga
    11701 ccggtacggc caacaacttt gcaactgcgt tacgccttcc tatggacatt atgtccgcca
    11761 tgcaagtttt aaaaaatggt attcctcaaa aaataagtct tggaaaagca aacggccgtt
    11821 actttactga agctgcgggg gtaggtatct tcgctgatgc attagctctc tacgaagggg
    11881 agcatcataa acagtttttt aaaggcttac tcacacttat tcgaatcttt ttaaacgttc
    11941 gcggctacaa tttagaactc gaaatagatg gccaaaagta taacgaaaaa tcagtaatgt
    12001 gcacggtcgc caacaccttc cgaattggct atgcctttcc agttgccccc gaggcaaaac
    12061 tcaccgacga tcagctcgac atcgttttgg taggcgactt aaaacgatct gaactcctac
    12121 cctacttccg agccatgcga gcacaaatcc acccgaccct gcccaaagtg acaacgcaaa
    12181 aagtgaaaac cgtcactatc cgtgcccgcc gaaaactcaa tgttcatgcc gacgaccaaa
    12241 taataggtac aaccccgatt acaatcgaaa cacaccccgg tgcattaacc gtcttactcg
    12301 atcgattata aaaaacaacg cccgccgtaa agtcaccata cagcaacctc acaacgggca
    12361 atgcgacgac agaagagcac ttctatctag agagcatacg ctcgatttca tcttgaccaa
    12421 ccggcatcac cccccgcatg accctcatcg ccaggttgag gcgtcgcccg tttccgaact
    12481 tgctagccat aagtgcggga aaccccttat agccggccag taccgcctca aaagactgaa
    12541 ggaagtactc gatctcctcg tccgttaccg tcagtggtgg ttcaacccgg ataacacggg
    12601 gcttgttcag ggtgtacaca gtgatgatac cgtgatcgac caacaactta ccggccacga
    12661 gagacccaag gagctggctc cctgtgcctt tcatcagaaa gtctagaccg gtagcagccc
    12721 aatgcttgag ctctacccca atcagcaacc ccttacctcg tacctcgtgg ataaccgatg
    12781 gatacttctc ctgcagactc ctcaagcccg tcaagagttt tctgcccttc cttcgtgcct
    12841 cttcacagag attttggttg acgatctcgt ggatcgaggc gatagccgcc gcacatgcca
    12901 gtgagttctc cccaaaagtg ctggtgtgaa gcgcaaacgt atcgaggttg ccgtaacacg
    12961 cgaaccagat ttcatcggtg gtcagaaacg caccgatcgg aatcacgccg ccacttaagg
    13021 ctttggccac acacagaatg tctggaacaa ccccttcctc ttcacaggca aacatccgcc
    13081 cagtacgacc gagcccggtt tggatctcgt cgacgatcag cagagtccca ttctcccgac
    13141 acagccgagc gacttcacgc aagtaaccgg tgggcgattc tcttaccccc ccttctcctt
    13201 gaatcggttc gagaataacc gccgccacat ctttctttct caagacaagt ttaagggcag
    13261 cgattgagcc gaaggggata cacacacaat ctggcaccaa agggccaaaa ggatcctgat
    13321 aacctttatt tccagttacc gagagagctc cgaacgtttt cccatggaaa ccgcgttcag
    13381 cataaacaat cttccggcgc cctgtataaa ggcgggctgt cttcatggct ccttctacag
    13441 cttcagcgcc actattacat agaaaactgc gcgtaagggc ttggcccggg ctaatgtacg
    13501 ccaaattctg tttcagcacc gctcccagag gagacagtga tgcctgcaga aaatttggac
    13561 gcgccatggc agaaacaacc gcctccagga tcggacgagg gtgatgccca aacggcaatg
    13621 cgccatatcc gcccttaaag tcaaggtaac aacgacctgt attgtcataa accttcgtcc
    13681 cataggcatg tgtgaagaga ggatcgaaat caatcatcat caacgtgccc gtaagcgcaa
    13741 cattgccatg cagaagatgc aatgctcggt tttgatctgg cgttaggtca aacgcctcgc
    13801 ctagcgtcaa tagacgcgtg cctacatccg acatagtccc cctccgaagt aaaactacag
    13861 catccgccaa gacaaaagaa atatacccta attattaatg ctatgcaact aatcataaaa
    13921 ctaaaaagca ggctaaacct gctttttagt ttattctaac cccttcttac acttcgtctt
    13981 ggtcgtcggc acgaagttcg ttgttggtga actccggttt atctttatac acggagtcaa
    14041 ggagagcgtc gagaatatta tgctctcggg ctcggttaac agattcatga gtaataagat
    14101 cgcctgtgtt aagtattacg ttgtcttcag agtcaagaat tacacgggta actggacgac
    14161 caagagcagc tttaatacgc tttgcttcga gagcctggga gctctgttct ttcatatcac
    14221 taatttgctc ttttattttc tcccaagcgc tcattgtctt ttctttcact gtctcagccg
    14281 caggagcaac agtagcttgc ataaactcag ggctatagtg cccttgggca actttctccc
    14341 gggctgctcc aattgtttct tgagttttcc ccttcgcctt atccacggca ccttgtacgc
    14401 cgcccgtttg ctgctcgtta agcatgtccc caatatcgcg gcggacaaat attagctctt
    14461 caccaatctt cgccacttct gcggccggca agtaagcctt tcctttgtac gtatcgctgc
    14521 tcaacccacg agacacttcg tacccatcaa tcactccggt atcaccttgg aaatagaagt
    14581 cggttacctt tcctacctct tcgccgtctt cggtaacaat cttatggccg cgaatatcac
    14641 tgccactatc gagaatccgt tttacctgcg gatcatcttt cgccatcagc accgtatctc
    14701 ctgtatccac ggttactgca tctttaccaa tggaacgaat tgccgagaga ggcaaatggc
    14761 gacctcgggc tagtatccag ctttcatcga ccagaaatcc aagaaccttt tgattaaagg
    14821 catcatggat aatatcgtat actttataaa ccgctttacc gtcggagtag ctcatgattg
    14881 ttttgccaat aatatctttg gctttaaatg attcctgttt attcacttgc tccgcggtaa
    14941 aagggttatc accatgtgaa tcacttattg agctattggc agatgattcg ttagagttcg
    15001 caaccgcaag attaacttta cgtgcccctg cgtcctgaag aacttgtgtc acttcgtctt
    15061 gtgtcttttc cgttacggga agagcaacaa ccacccctcc ccgattaact ccctctgaaa
    15121 attcgtttgc atcagcctct gacaacccaa agttacttaa cgcaccaact aagccacctg
    15181 ccataccgcc ggtaatggca ccagaaactg tagccgccgc caaaccagtt aacccaagcg
    15241 ccgcagcaaa tggtccacca attaatattc ctgcgagtgc tggtacaaca ccagcaccag
    15301 ccagtaaccc ggctaaacca ccaagcgccg cgccggatgc aactccctct gtaacacgat
    15361 cttcgggggt atcactatct acccagttac ttcgagtaga atttgaagca atcaccgaca
    15421 tattttcagt tcgatatcct cggcgagaga gttcaataac cgcgctatca gcctggcttt
    15481 cggtatcgaa aagggcgagt aagttctttt ccatataacc tccatcgttt gggtaagtaa
    15541 tctcacttta gttactttta cctttaaggt cattggtaat actgattaaa ttaccaagcc
    15601 aacagcaaac tcgcttggaa ggtacataaa agagctcctc gcccttcctt acagacatga
    15661 agaagggaat taggtataat ttgagtatgt ttgacccgaa aaaacgtaat caccacatcc
    15721 aatacgctca aaactttttg aataatccga gcctcgcccg ttacgtggtt ggcctctcaa
    15781 atatcacctc caatgatagc gtggttgaaa ttgggccagg caaagggatg ctcacaaata
    15841 tcttaattaa aaaatgccga caccttaccc tagtggaaaa agatcgagaa ctcgctttgg
    15901 agctgcgcca gagatatgcc gaggcgccta cagtaaaaat cattcatgct gacgcgttac
    15961 aattccggac agaagaagct aattacaagg ttttctcgaa ccctccgttt aatattttgt
    16021 cccaggttat taaaaagttt gtttttgcta aaaacgcccc gcaagaaatg tatctcttta
    16081 tgcaaaaaga agcccagcaa cggctcagtg gtaaatcagg cgaaaatcag ctctccatct
    16141 tgattaagag tttctacacc cttaaagagc ttcacaaatt taaaccgagt gattttagcc
    16201 ctgtgccttc aatcgatgtt aacttaattg ggttctacag caaatcaggt aatcccattc
    16261 tcccagttga taaacaagaa tacattcttt ttgtccaatt tgggttccaa caacaaaaag
    16321 caaccttaga aaagaatttc gataaagttt ttacccatga acagtggaaa cgattagcaa
    16381 aagatctcaa cttttcgctt aagtctctgc ccacagatct taaacctgat cactggattg
    16441 gactgtttcg tttctataaa attggtgtta ctcccgaaaa acagcggata gtgcaagcct
    16501 tcgcaccgcg aaaataaaaa ctcccccacc ttacggcagg ggagagaacc gtttctttag
    16561 atgaagacta acgaggagcg gaaggcggct ctccaagcgg aatctcgacg atcgcttcgc
    16621 cgtaaagccc ttttacctcc agtttaaggc catacgtacc gagcggctca cgcttagcca
    16681 ccaccatcag ttcttcagct cgacacttga gaaaccagcg cgtcggactc gtgtctggat
    16741 tggcaatgaa tccgacatac aggcttccct gttcatcgat catctggatc cgtcccagac
    16801 agacaacgga gatatgaccg gtgagtgagc atacctctgc ttcattgaga aagaacactt
    16861 catcgccaac cgtataggtc gttgaggaac ccgccttcca ccgtgtggca acaaagcgct
    16921 gagagtagta accaactgac ctgttcggcc acgtgcgggt cacaagtgaa ataaatggtt
    16981 cgggagtaac ctccagcaca ttatctgcat cataggagac tgggttactc acctctttta
    17041 cggttatctc acgttcactt ccatccggca gacgaacgag gaaaccggct accttgctca
    17101 cctggtccct tgtcacttcc cgaacgtgga ttattcgcgc cattaccgct cgtttccttg
    17161 cgataaataa cagcattcct ggagcaacat tcgtgccacc ccaaattgtt tggcacggta
    17221 ctttctccat cgtttttctc actttctacc caaccccaaa cgacacattg agtatatatc
    17281 tcgctagcgt tttgtcaagt catcattagg aaaccccctc tggcagaacg ttctgccaga
    17341 gggggcaatt agagatttcg gaaaagagcc agaagtgcta aaagcagcac ctcccgaact
    17401 ccaaacgtca acggatgtcg cagtacccac ttgtcgttat cccaccctag agagtgtgcg
    17461 ctcgcgtgga agactgacgt aagccggcca agacgacggc acgccagcaa aagaacccgc
    17521 cgcatatgca tctcctctgc aagaatcagg actggacgcc cttctccttc tagcttcggc
    17581 actacatctt gcagataacg ggcctgggca aacgtattaa gcgtttcatc ctccacgata
    17641 attcggatat gtggcgaaat cctcccgtaa actgcttctg cagccaacgt cccttctttc
    17701 ttcccattgg attcccatcc gcccgtaagc acgagattgt cgacctgata caggttgcac
    17761 agctcaacgg ctcgatccac aatcgccgtc attttcggtc cgagtgcccc atttgtgagg
    17821 ctaaacggaa taacaataat ggccaatagt cgagtatctc cgtcgggcag atcgtctggg
    17881 acgacaagct gatctcgtaa ccatactcca agttgatgga gtatccccct ttgcgtcgtc
    17941 atcttttccc cttataatcc aatataagaa atatactatc acaatcacag agcagtaaca
    18001 agccacataa acgcttggaa cattcaattt aaaaaatatt taacttttac aacatactat
    18061 tggtctaaaa gtatggtaga ttcatagcat gcgtatcctc attatcgaag acgatcgaac
    18121 attagcgaca agcttacaag aacagctgag cgaagtttat aaagttgata ttgctcacac
    18181 cggcgcgcgt ggccagcgtc aagccgaagt aaccgcatac gaccttattt tgctcgatct
    18241 tgacttgcca gacatccatg gaaaggacgt ttgctttgaa ctgcgtaaaa agggcattaa
    18301 agcacctgtc ctgattgtga ccggagaagc tgatatcgaa ataaagacaa gctgcttaga
    18361 cgccggagca gacgactaca ttaccaaacc atttcacagc caagagcttt ttgctcgtat
    18421 ccgtgctctt ttacgaagga atccagacac cgccttatcg aaccatttgc gagtgaaaga
    18481 cttagagctt gatccagcca cacgcattgc gactcgtcag gggaaaacca tcccgctgag
    18541 acgaaaagaa ttcgatatcc ttgaatactt aatgcgtaac ccaaaccgag ttttaacccg
    18601 ggatatgatt ctcgaatatg tatggggaga tacgagcgaa ccatttacca acagtattag
    18661 cgtccatatt aaacatctac gagataaaat cgattaccca ttcgaaccaa aacttattca
    18721 gactgtccac agtctgggat ataaaatcca aggatgatat ctacggcgac gagatcaaat
    18781 ttaagaaaga aaggaggtga ctaaaatgaa caaactatta aaacagatct taaccaataa
    18841 gaatgcccgc gatgcacgtt acattaaaaa agaagtgctc gcattaagtg atgttggcgc
    18901 accttggcaa gactaaatta ccgtaaggag ctggactacc tcacagctcc ttattaaata
    18961 aggtataatt tatatatgaa aatatctctt cagcgacttg atcacgtaaa aaagctgctc
    19021 attgcaccca agggagccag cgaagataca cgccgccggg agtacatttt aaacctcctc
    19081 ctattgggat ctattgctct ttctgccatt gctctctttt ccgcctttct taatgtgctc
    19141 gcccgaagcg acggctaccg aggagaatca ctgctagtac tgtgtatacc aatgggattt
    19201 tttctctgcc tctatgcact ctcgagaatt cgttacccta aaattgccgc cagtgtcttt
    19261 attgctacat actatatatt agctacctac acgcttgcaa aatggggcat ccttttaccg
    19321 caaggtattc ttatctacgc tgttatcatt gttatgaccg gtgtactctt aagcacaaat
    19381 gctgcctttt ttgtgacctg tttgctgagt gtcacacttc tcactctccg cgtctttgag
    19441 atccaaggca cacttcatcc caacttatat tggatacacg aatcaggcgg tattgatgat
    19501 gcgatagtat tcagcgcaac gctgtttatc attctcattg tttcatggct ttctaatcgg
    19561 gagatttaca agtctctcga acgagcacga aaatctgaag cttcgcttaa agaacaacga
    19621 gattcgcttg agattattgt tgaagaaaga acacaagagt tacgggcaca acaaagagaa
    19681 gagaatatcc gcctctaccg tttcgcagag tttggccagc taacatctgg acttctccac
    19741 gatctcacca ctcccttaac gaccctttct cttaatttag aacggttaag caatcacgaa
    19801 gattcacaaa caattaaacg agcgattgat gcggcccgac gaatgcatct ctatatagat
    19861 gcggcgcgaa aacaattgtc tccaaatgtg gaaataactt gttttaagcc gattgaagag
    19921 attcaggaag cgcaaaaaat attagcccat cgcagccgga aggcgcatat aaaattcacc
    19981 tgttcgatga gtaaagatat caaactttac ggcgacccga taaagtttaa tcgtctggca
    20041 acgaacttaa ttgccaatgc catcgatgcc tacgatacaa gccctcctgc ggtacaaaaa
    20101 aatatcctgc ttactctcga gaagaatgat accgcagtta tttttaaggt agctgatcag
    20161 ggctgtggta ttcaaaaaaa agagcaaggc aatatattcc ttcctttcta cactacaaaa
    20221 agtaaccaga aaggaattgg catgggactc gccatatgta aagaaattgt cgaaaaagat
    20281 ttccatggct caatcgaggt aagtagctcg tccgagacag gaacgacatt taccattacc
    20341 atacccatta ctcatatcca tgaacaaaac agctaagacc ttaggcgttt cgttcctcgc
    20401 tgcctcccag ggacagcatg gcggatactt gagtcattcc agttccgatc cttctacctt
    20461 taaagataca tccacatatt caacaacatt tgcctcagcc cttattcttg actgcttgca
    20521 taactgtcca cacacagaaa acatcaaaca aaaacttgta tcatttcttc tctcccaaaa
    20581 gactccgtct ggttcgttta attactggga tagaaatgac ccgattactc aaacattgcc
    20641 ttatcccgat gacctcgatg atacattctg tgccctcgct gctctccatc gagcaaacaa
    20701 gaaagggaca aatggaaaaa cactagctca agctgttact cttctcgcac tcaccgaaat
    20761 caaagaaggt ggcccttata aaacttggct cgtacctgac aatgccgcgg ccgtgtggca
    20821 cgatgttgat cccgctgtta acagtaatat tgcctacttt ctctctctcc aagaggtaga
    20881 actcgtgagc cttgtagagt taaccgaaaa cgcaatacaa tcggccaact atacctcgcc
    20941 ttactaccct aacgaatatc ctctttttta ttttatcagc cgttggtatc gaggtcgctt
    21001 gcttacatca ctcgccgatg ccattttgtc ccgacaacta tctgacggtt cgtggggcag
    21061 cccccttaaa accgccttgg ccctgactag ccttctccgt ctcactcttc ctgtccccaa
    21121 aatacaccag gcagttagtt ttctctgcaa aacacaaaac tccgacggca gctgggaagc
    21181 ggaaggattt tgctacgatc caatgattca aggca
//
LOCUS       DASWYB010000037        25656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_439155, whole genome shotgun sequence.
ACCESSION   DASWYB010000037 DASWYB010000000
VERSION     DASWYB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25656
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439155"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VGE59_02165"
     CDS             complement(<1..172)
                     /locus_tag="VGE59_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEY1074482.1"
                     /translation="MLHSMRLHPRPFDNIKSGKKTSELRLNDEKRSAIKIGDMIEFTS
                     RVNPDEKLVTKVT"
     gene            complement(193..984)
                     /locus_tag="VGE59_02170"
     CDS             complement(193..984)
                     /locus_tag="VGE59_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY1074483.1"
                     /translation="MKLINCFIIRNSTWLIPLITFVVVFLCIWLGTLQYNSNRSILSL
                     AGTLIVLLVAAPLLQLFIFRFRLRFKRYTGEHKEKLVKYCEQRGVTHVEFFEIKGRLP
                     NAYATGNRKHKRVTFTSRLLEECSYEEVEAVLAHELGHHVNDDPQSTLRVIVFIFFFA
                     SWFNGEMVRATQWNLLVVSILSSVIFYLLFLVRSRRNERRADMYAATDPKAAKGLIAF
                     FKRSQDIYKKEGVWIPRPFPLILRITKTHPTLDERMKVLGNYKSN"
     gene            complement(994..1314)
                     /locus_tag="VGE59_02175"
     CDS             complement(994..1314)
                     /locus_tag="VGE59_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074484.1"
                     /translation="MKELVKLVVFVPEENADQVRQALGEAGAGRVGNYTFCSYSVKGV
                     GRFKPGDGANPHIGEVGKLEMVDEERIEVACEKDQVNEIIQVIKNVHPYQEVVIDIYP
                     MLSL"
     gene            complement(1334..2098)
                     /locus_tag="VGE59_02180"
     CDS             complement(1334..2098)
                     /locus_tag="VGE59_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY1074485.1"
                     /translation="MESTYSEIIEFITDAGKRLKEESGKVKDIGITKQYLTEEDLRIE
                     RGLTDIITKLGDDHSVFAEEENDNFQATSNVWVIDPISSTKNFIEGRPHYAIVVSHIL
                     DGQAQFAAVYDPSIDKLFTAYRGKGSFMNGVSVNIDNKVENKKVIFRISSQWRETDSL
                     KELEDALANYELATNTDSVAIHYCDLLRGRVSGVICFAKDSFPNFAGSFLVNEAGGKA
                     TNREGSSNLNPTDRIFIAGSQSQYAKLFSIVTQFIK"
     gene            complement(2101..2523)
                     /locus_tag="VGE59_02185"
     CDS             complement(2101..2523)
                     /locus_tag="VGE59_02185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY1074486.1"
                     /translation="MNQTVQAFGYPESLIKEYDHWVVLLRPQQVTIGSLVIAAKSDVT
                     NLGKLSSDAWTEFSLVTKETEELLRNTFGAEKFNYLALMMVDPNVHFHFIPRYSQPVA
                     LGNQEYTDADWPMKTELKPIEVSPEQMEILKAMLKSEV"
     gene            complement(2520..3071)
                     /locus_tag="VGE59_02190"
     CDS             complement(2520..3071)
                     /locus_tag="VGE59_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074487.1"
                     /translation="MKLYRFSPIGNKEQLLDAIKYIHESCYKLCKQSFGYYLPNAGNM
                     GVFCHYDDEYSYLVKLREEMTEPSDNVDQKYFRLRKPIIIPADGEIPEATYTYLYIRK
                     PDPYRHHVGDVDFYVESNQYTDLKQSLLDGKERKGARIFPRGDLDMIELYDPDSDVLA
                     YVSQKTMTEKVRVKVNLKTKEML"
     gene            complement(3077..3664)
                     /locus_tag="VGE59_02195"
     CDS             complement(3077..3664)
                     /locus_tag="VGE59_02195"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1074488.1"
                     /translation="MNKPLSKQPLPENAKKVFNGIIFDVYQWEQLQFDGTVKIFEKLK
                     RDDTALIIPILEDGNILLLEQEQPGKPPFIGFAGGRIDKGEDPLEGAKRELREETGYE
                     ASEWELIDAVQPVSKIEWSVYTYVARGCRPFGSQSLDSGERIQFKPISFDGLVEIMAR
                     DDFHDKEGMLGRFALRALYDPSQREQFRKLLYGME"
     gene            complement(3661..4632)
                     /locus_tag="VGE59_02200"
     CDS             complement(3661..4632)
                     /locus_tag="VGE59_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY1074489.1"
                     /translation="MKIAIDILRHKVETTLQKKFTKEESERIAEYLIWAEMAGVKTQG
                     IIKMTGKEPLQNITPQHEMRVERDTKLSQLINAGAHPAPLVSQVATDAVIAKAKEHGF
                     GIVGVHNTFSSNGSQAFYVERIAKEDLIGIMMSRSPASTAPFDSIDPLFGTNPIGFAF
                     PTNEEPVVFDMATSAMTFYGLILAKARGESIPEHMAIDSEGKPTTDPGAAMDGALLPF
                     DRSYKGSGIGMVVEVMAGPLVKAAYGQLEGEWGTLFIAIDPNLLVDTAEFKTNCSDLV
                     RKMRQARTKSGSRMRLPGDRTHMAYQEALSTGMVEVEDVILKQLGYV"
     gene            complement(4635..5120)
                     /locus_tag="VGE59_02205"
     CDS             complement(4635..5120)
                     /locus_tag="VGE59_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1074490.1"
                     /translation="MEINIRLMNESDLDILAEVYCEAYRVFDVGERWEKDSARDLLAY
                     WFKHQPDLAFVAEDDSKVIGAFIAGIKPWWNGNHLVDGEIFVHPDYQARGAGSRLLKV
                     MFETAIEKYDADVWEALTFADHEFPLRWYKSLGFEPVKDLVLFSGDMKKALVRLSKKG
                     F"
     gene            complement(5183..6202)
                     /locus_tag="VGE59_02210"
     CDS             complement(5183..6202)
                     /locus_tag="VGE59_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY1074491.1"
                     /translation="MKPKILLLSMGGTIFSIVENNVVRTAHYIQELMNQVPRLHDIAE
                     LDFEVVVNLDSTNVGIDEWMLLHQRITEKYDQYDGFVVAHGTNTMAYTASALAFSLGS
                     TLNKPVVLTGSQLPLSVYGDDAHFNLEHSVRACCLAIDQHICEVMVSFHDVVLRGCRT
                     VKVSESNFAAFSSPAFPPLAQVTANGISFSGLVRKRQGGIFERRSPQFSSSIICIDIV
                     PGLDPMLLTSLVERGEIKGIVLKSHGAGSVPDQGRGSFLPFIESMVKQDIVVIITTKF
                     LGGNSSKMTNDEPAVRALEAGAISSRDMTDVAAQIKLMYLLGNGNNASQVRDRFTESF
                     VGEVS"
     gene            complement(6237..7244)
                     /locus_tag="VGE59_02215"
     CDS             complement(6237..7244)
                     /locus_tag="VGE59_02215"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEY1074492.1"
                     /translation="MKNIFRGVNLGGWLVLEKWITPSLFSGYTAEDEWSFCRELGNRA
                     FEVVDRHRQSYITRDDFAWIAERGLNAVRIPIGHGIFGDAPPYLGAIEYLDFAIEAAK
                     DYGLSVIIDVHTASGSQNGWDHSGQKGAINWPNDVAHRDQSLFVLKRLGERYQSYPHV
                     IGIELLNEPHWTIQKPILETYYRHAYETLRREGYQNKIVIHDAFDPPAWIPFMTNSSM
                     GDVVLDMHMYQCFSDEDKALNFMGHLEKTYGEWGRLISQIQEKVPVVVGEWSLGLDPL
                     SLKGLSDQEKGEAFKSYGKAQLEVFEKAQGWFFWTYKTEDMPHWSFRDCVTQGLLPDH
                     F"
     gene            complement(7281..8297)
                     /locus_tag="VGE59_02220"
     CDS             complement(7281..8297)
                     /locus_tag="VGE59_02220"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1074493.1"
                     /translation="MDKPILYILCGLPYSGKTTLAKSLIRKLGCAYVSIDVIRDQLGF
                     SWEENHKVTADNWRQIFKASYQDMRNKLGRGQSVVFDSTNHDFDSREKLRTYAAGVGA
                     DAKVIFIDVPVNVIWKRWEENQNLKTRSHIAKELVEWTIDHFEKPENSEQVIRYEFPE
                     DGEAWVLKNFANNSSWINSKAEIKVSPVQGKGLFARELIKGGEEVVRWGGEYTDRVGA
                     EVARNTGKLVMQWDDNLFSIEDRGDDDAYFINHSCEPNLWMKDAYTLIARRDINPGEE
                     LTADYALWEADENYVSRWECKCGSSRCRHKITGKDWRRPELQKHYKDHFSPLINKRMQ
                     RKEM"
     gene            complement(8364..8570)
                     /locus_tag="VGE59_02225"
     CDS             complement(8364..8570)
                     /locus_tag="VGE59_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074494.1"
                     /translation="MQKVFGSAYKCDYVLTDFRFPPDKEHELEKLEERKIADAKAIFA
                     TIADGDDATILKVTSQDLQNNYLK"
     gene            complement(8525..8899)
                     /locus_tag="VGE59_02230"
     CDS             complement(8525..8899)
                     /locus_tag="VGE59_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY1074495.1"
                     /translation="MTNKLNNHYDVALVLGSGIKPDGAIPESAKIRTQKAADLLTDGA
                     VGHIIFSGKWTYGLAYTPPLTEAEAMAQYGKSLGLEASQYIIEKESVTTVSNFCNIKR
                     LMKEHSLTCKKFLVQPTSVIMS"
     gene            8986..9297
                     /locus_tag="VGE59_02235"
     CDS             8986..9297
                     /locus_tag="VGE59_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017930273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY1074496.1"
                     /translation="MNKEEIIGGTVHELPQDLKDALLATPAARTAWENLTPLARNEWI
                     CWNITVKQPETRKHHIERTIAELQEGKRRPCCWIGCIHLPDKQISPSVQWVLNKQSKS
                     K"
     gene            9386..10318
                     /locus_tag="VGE59_02240"
     CDS             9386..10318
                     /locus_tag="VGE59_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY1074497.1"
                     /translation="MTPEFNKTDTSELDPEAIEAAEIQALLPTAAHFFGLGSIQNIKP
                     TPFGIGNESFFVSSEQGDFVLRVLWEQTIEGLEKEVAIEQQLAHAGVLSAQLLQGENG
                     NYYFTDGKYTITASRKIEGIHPHEITPDLLNQVGTTMATFHTSVTEIPQETSQWFTLD
                     NARIEVAKVSSDEESKSAIENNVTTGSSIFNRDLPTGFIHGDLYNGNLILTPDNRLAV
                     LDFETTERNVLIIDIARTILDMCADDVTVNTQSVEALLDGYEEIRQLTDEERESLSDA
                     IRYVSGAMAAWLIVEREQKEVAQNILQRAESLRS"
     gene            complement(10407..10889)
                     /locus_tag="VGE59_02245"
     CDS             complement(10407..10889)
                     /locus_tag="VGE59_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEY1074498.1"
                     /translation="MDDFIIKNLDLKNIENLFQSHKQKVSRSLVGKSVDIQHVGATSV
                     IGLISKGDLDIQIRVSRDDFEPTKEVLSKEYEPAQEENWTKTFASFKDDSGETAVGLQ
                     LTIINSEEDSYFYKLRDILNEDVSARESFNQLKREYDGKSMDDYRKAKSKFIEGLFSK
                     "
     gene            complement(10972..11382)
                     /locus_tag="VGE59_02250"
     CDS             complement(10972..11382)
                     /locus_tag="VGE59_02250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1074499.1"
                     /translation="MIEFKTVKDISAHDLNSLFESIGWGSRVKEKWKGVLAASSFVVS
                     AWDGDLLVGMGRILEDGVMCMFYDIGVRPNYQNQKIGRKLMDLLIEQVKDKSYVSIGL
                     FVWEENKDASEFYEKFGFEKVETGMELVKYMERE"
     gene            complement(11453..12157)
                     /gene="uppS"
                     /locus_tag="VGE59_02255"
     CDS             complement(11453..12157)
                     /gene="uppS"
                     /locus_tag="VGE59_02255"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY1074500.1"
                     /translation="MPTLIVPDHIGFILDGNRRFAKLRGLRPWRGHEFGKKKVQQLLD
                     WCGEFGVKELTLYTFSMQNFGRPKEEVDKIMELIEKSVDEFIDDERIHKQGIRIKVIG
                     HVEMLPQSVQAAIRRAEEATKNYSNFTLNLCIAYGGKEEIFSAAKMLIKKVLEKAIDL
                     KDITLESFESQLQLQSEPDLIIRTGGEHRTSNFLSWQSGYSEWFFVKKLLPDFNKQDF
                     QNILTEYADRERRFGK"
     gene            complement(12212..13237)
                     /gene="mraY"
                     /locus_tag="VGE59_02260"
     CDS             complement(12212..13237)
                     /gene="mraY"
                     /locus_tag="VGE59_02260"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY1074501.1"
                     /translation="MEVFSINLSDLKFVFLLGTLSFVIAMLWTPIFTDFLFKNRLGKK
                     IRQTAFDDKKAPIFYSLHKGKENTPTMGGLLIWITAGVITLLLNLDRAGTWLPLFVLI
                     ATGLIGAADDLMNIYGIGPHNGGMQFRVRFLLYTIVAVVGAWWFYSKLGFSFFHIPGY
                     GNVDLGIWYMPFFVAVLVWTAFSANQTDGLDGLAGGVMAIAFSAFAFIALFQGKVELA
                     ALCLTIVGSTLAFLWFNIYPARFFMGDTGIMPLGMTLAVIAFLTNSVFVLPVIGIILM
                     IEGVSTILQIGSKKLRGGKKIFRSAPIHHHFEALGWPETKVTERFWIISAIGSVVGLV
                     IALVGKG"
     gene            complement(13258..14496)
                     /gene="murF"
                     /locus_tag="VGE59_02265"
     CDS             complement(13258..14496)
                     /gene="murF"
                     /locus_tag="VGE59_02265"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY1074502.1"
                     /translation="MVKTILFRMYTGILAWEASIFRNRYQGPVIAITGSAGKTSTKEI
                     LGKLVRKAYGENVFVTPKSLNAEIGVPLTVLGFKEEPRGLIAWCMAPLQGAMTAFLGK
                     VPECMVIEVGAEHKGDIARFSRLIRPTHAIITNISQVHSAYFGSLEELKKEKLSLLHF
                     VDPKGVIILNGDDDSLNKVMIQPTQEKILIRIERRADYFVSGVKVTLQGTQAVLHHHN
                     RTQRIHIARYGEQHMYGVMFAAALGDNLGISPAVQLQTFKEIRPLPGRGMLIEGKRQS
                     IIIDESYNADPLAMRGSLELLRQLPASKRVAILGDMRELITPDPIHREIGAFARQCAD
                     YVIGVGPLSKLYKPDEWFMTSPEAVASALRQLGPDVIVLVKGSQNTIRLEKLVKELMR
                     SPEQASKLLVRQGPEWNHRP"
     gene            complement(14621..16330)
                     /locus_tag="VGE59_02270"
     CDS             complement(14621..16330)
                     /locus_tag="VGE59_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY1074503.1"
                     /translation="MDFLEWQKRLSILLSVGGLILLSFTGRLYQKTVIEHGQTVEEAE
                     SQYAYRKEVEGQRGEIVVAQDDKSYFPLATNERRYQVLVVPKNIKDRKQTAEKLASLI
                     GMTEQEVYDKINNDKLYIPPLKKRLTREVADRIAQLRLRGVVLLPELVRVYPEQTLAA
                     QVLGFVNGEGKGNYGIEGALDLTLRGASGYQTGEKDSHGRLINVGDEVKAQDGSTVVL
                     SIDREIQHYAEQALADAIKEYQADSGSVVIMNPKTGGIVALANAPTYNPNEFNKVASD
                     QSAVYQNPSLSSVWEPGSIMKPIVMALAVDKGLVEPETQETFAASVKVLNHTIWTAEK
                     KAFGLETMTQVLENSDNVGMVWIANKLGNNGEYDGLKQFGIGTAPDMNLSNVVGGFMP
                     GLKNWNDLTRATISFGQGVSTTPLQMAIAYSALANKGVMMQPYLVEKVIDEKGVLTET
                     QPKELGRVVSETTSEKLGRMLEAVVVNGHGKHAAVPGYRVGGKTGTAQVVKPEGGYYD
                     DRHIGSFAGYFPLTDPQFVMVVKIDNPKATKFAESSAAPTFGRIANWILSYKQILPDK
                     PMQ"
     gene            complement(16381..16722)
                     /locus_tag="VGE59_02275"
     CDS             complement(16381..16722)
                     /locus_tag="VGE59_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074504.1"
                     /translation="MIPVTYGATTVNSRINRPGRAAARIGFFSVRMIGFLMLAILALL
                     YIAQSSQATTKRLDVQSTRQQADLLTTEEDQLRLEAKRLQSLDTISQSATTLQLEPVQ
                     SVEFLSPAGSN"
     gene            complement(16736..17587)
                     /gene="rsmH"
                     /locus_tag="VGE59_02280"
     CDS             complement(16736..17587)
                     /gene="rsmH"
                     /locus_tag="VGE59_02280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY1074505.1"
                     /translation="MKHIPVLMNEVMVALQPRDGGNYIDATLGDGGHTEALLEASGPT
                     GRVLGLDQDPGQLAIARERLTQFGTRAVLVQSRFGDMQHVAEAQGFSPVDGILFDLGI
                     SSRQLDDPKYGLNIIDDTPLDMRLSPELDMSAADFLTRGNEREIADVLYLYGDRHNSR
                     TLAHKIVNYRRKQVFQVAHDLKEALNLWRPMELAPIFQALRIHVNREYEQLEKALPAA
                     IRLLKPGGVLAIISFHSGEDRIVKRFLLSQRELLEVSKKIIQPAFTEVKKNSRARSAK
                     LRLAYKK"
     gene            complement(17877..18308)
                     /gene="mraZ"
                     /locus_tag="VGE59_02285"
     CDS             complement(17877..18308)
                     /gene="mraZ"
                     /locus_tag="VGE59_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY1074506.1"
                     /translation="MFLGEYEHTIDQKGRLAIPAKFRTALGEGAVIARGLDNCLTLYP
                     KKEWERLAERVASLPTTDANARSFARFILAGAVDVEADKQGRVILPAYLRQYAELGAN
                     VIVAGLYNRVEIWDKARWSDYSADAQANSSDVASRLSDLGI"
     gene            18609..19106
                     /locus_tag="VGE59_02290"
     CDS             18609..19106
                     /locus_tag="VGE59_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074507.1"
                     /translation="MQHYTPLKLLYVAFLGLMLALFTGVLISVAYPGPKAPEDTRTVP
                     TVKTLPETGTPATPSPEEIAQQKEYDQKWKDFQDRQKTYERNVSLIALGASVIMLVVG
                     LVLATKIDILADGFLLGSIFTMLYSIIRGFNGESEWQRLIVTTAGLVLALVLGYVKFI
                     KPSQK"
     gene            19200..20207
                     /locus_tag="VGE59_02295"
     CDS             19200..20207
                     /locus_tag="VGE59_02295"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEY1074508.1"
                     /translation="MGSQSLAQTVFEDAVFEHYRGYLRRNDPQKLRSQSRTDDSRGIG
                     DILKRYPATAPLSDELAAVIRGFRDVECLLADYSSDIARIVRHEPAQVQFTMFWTGEE
                     AIHGFALDEVLIAYGANTPREVRDDKARLLTTCKWDPSKVDVRFLDPWYGPMCYVTLQ
                     EKVTGNGYRWLRRSAEREGHIGLAKICQRLAEEEFRHHGFYYGMAKLALSYQPDKLKR
                     QMTEVVTDFAMPGRYAIPFQQAETATPDNPLPSALPAYDEWTKTAQRLGIVPDPLFAV
                     PLAARLRRKSEVLRLDEHTIEGQRVACWNEHVASVLQILKGLGIELPRELRYLAPIPV
                     A"
     gene            complement(20257..21609)
                     /locus_tag="VGE59_02300"
     CDS             complement(20257..21609)
                     /locus_tag="VGE59_02300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY1074509.1"
                     /translation="MSLHSLEPIVNLTKRKGFVVQSSEIYGGVASLFDYGPLGVELRN
                     NIKQFWWKTFVHGRDDMVGIESSIIMHPRVWDASGHTETFTDPLVECTKCHFRFREDH
                     VPTKEHNPKFWKCPEGADHTFAEPRQFNLMLKTYLGATEESASAAYLRPETAQGMFVD
                     FKYVTETSRTKIPFGIAQIGKTFRNEIGLGNWLFRLREFEIAELEYFVKPGTDEEHFM
                     AWVERWEAFYEACGIPREHIRRDVKAKEDLAHYSKGTIDFEYKFPFGWDELGAVANRT
                     DYDLKRHAEYSGKDLSFFDPETKEKFVPYVIEPTQGIDRLFVAILCESYKEYPNGRKG
                     EEGETLAEPEVVLHLPPIFAPYKVAVLPLVKKDGLAEKAKELAADLRKHVTTYYDESG
                     SIGRRYRRQDEVGTPWCVTVDHQTLEDGTVTLRDRDTMEQERIKLDELSQLIYFKLNG
                     "
     gene            complement(21750..22439)
                     /locus_tag="VGE59_02305"
     CDS             complement(21750..22439)
                     /locus_tag="VGE59_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEY1074510.1"
                     /translation="MAHGNMSLSQEPRSVVLPRREAFLRELGFDLPSAQMPHVVAPVM
                     SHSDEIRTVPHEGVWLAPDCDVLVTQGPSVLVQAIADCLGLVIVDPVTRTIALAHAGY
                     QGVGLDVPTKTVRYLERRFGVNPSDLLVAMGPSWQGSDGEGAWMDTRGPRLDSPRWNP
                     YIRETGGGIVLRWVDRAYDQLVAAGVLRERIERCPIDTYSGSDFFSHHRWVGWGKEGI
                     DGRHLVAVGIV"
     gene            complement(22594..23565)
                     /locus_tag="VGE59_02310"
     CDS             complement(22594..23565)
                     /locus_tag="VGE59_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEY1074511.1"
                     /translation="MSYISPPRLQSGDLVEVIAPSASLSRIPEDEQARAHHILSQLGL
                     RVRFSSHARLSDGFYASPIEGRLKDFHAAFADPEVKAILPVTGGYNANDLLPYIDWEL
                     VKNNPKILCGFSDITTLTTAISVACGFVTYAGPVYASFGAFTGIDEVVDSFYNATFTS
                     DPYSLPSSRAWSDSRKSTELFLNPGAVVLNEGTAEGQLWGGNLLTFNLLQGSKFFPSL
                     QDAILFLEDDDAEHFARIARGFRALSLQPGFSQIKGLLIGRWQRGSSMTDELLRHLLL
                     DQLGLRHIPIITGLDFGHTVPLLTLPVGGTVRIDAQATGSRIQILTH"
     gene            complement(23570..24673)
                     /gene="alr"
                     /locus_tag="VGE59_02315"
     CDS             complement(23570..24673)
                     /gene="alr"
                     /locus_tag="VGE59_02315"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY1074512.1"
                     /translation="MHTWIEVNPFALIRNVRAVREAIGEGTALYAVVKANAYGHGLEL
                     ASDAFWRAGVDGFVVTECKDALFLAEKYPRTPVVLLTPAEKHDIGTLVQREIRLVATT
                     PQHLSDIQLAAQRHGKQALVHLEIETGMNRLGMAIPAATEVIRSLSNEKSPVHIEGMF
                     THIYHASDREVTERQAEVMRQFQFDLQQEGLPVPYVHVFASESLLGTEHDYMDYLFDG
                     VRFGRMLYGAKRPNFTTEPALTWKTQILAIHRIQAGETVGYNATYTSDKTQLIATLPV
                     GYADGLDRKLSNKGFVLIGGKRCPIVGEVCMSHTMVDASAVFRPQVGDEVVLLGKQDT
                     IAITAEEMAEFVGTIDYEILARLSPAIPRMKTK"
     gene            complement(24657..25412)
                     /gene="recO"
                     /locus_tag="VGE59_02320"
     CDS             complement(24657..25412)
                     /gene="recO"
                     /locus_tag="VGE59_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY1074513.1"
                     /translation="MQTVKDRAIILRRVPFGEADLMLTLYSEKNGKVRAIAKGARRIT
                     SKLLGYTELFTLVSCQLNFKTSIPIVSQMSHEQIFDGVAEDPTLYERLHILAELVDKG
                     CQEQEANMPLYRCLQEGIEQLVVSDHRLVLPSLILQVSRLLGFEPQLQLCAHCGTPLA
                     EDQRLVWSEAHGGIVSCSVGAAAGQVLSIDDIKVMRYLAKTPVSQVEKLRINSDMSAR
                     VESLLLRHVQYSLDQDFRTTRILKDLQSYAHVD"
BASE COUNT         7080 a         6443 c         5216 g         6917 t
ORIGIN      
        1 acgtcacttt tgttactagc ttctcatctg gattcactcg gctggtaaat tcgatcatat
       61 cgcctatctt aatggcgctt cgtttctcgt catttaaacg gagttccgat gtctttttac
      121 cgctttttat attgtcaaac ggtcgggggt ggagtctcat tgaatgtaac ataaaccgat
      181 tatacctaaa ttctaattac tcttgtaatt acctaacact ttcatccgtt catcgagcgt
      241 cggatgggtt ttggtaattc gtaagatgag aggaaacgga cggggtatcc agactccctc
      301 ttttttatat atatcctgtg accgcttaaa aaacgcaatc aatcctttag cagcctttgg
      361 atcagttgct gcatacatgt cggcgcgacg ttcgtttcga cgtgaacgaa ctaaaaagag
      421 taaataaaat atcacagacg aaagaataga aactaccaat aaattccact gagtagccct
      481 caccatttca ccattaaacc aactcgcaaa aaagaagata aagacaataa ctcgtaaagt
      541 tgattgagga tcatcattca catgatgccc cagttcgtgt gccaacaccg cctccacttc
      601 ttcataagag cactcctcta ataatcgact cgtaaaagtt actcgtttgt gttttctgtt
      661 ccctgtcgca tatgcgtttg gcaacctgcc ttttatttca aaaaactcta cgtgagtcac
      721 acctctctgc tcgcaatact taaccagttt ctctttatgt tcgcctgtat agcgtttaaa
      781 tctgagacgg aaacgaaaga tgaaaagctg cagaagaggc gcagcgacga gtaaaacaat
      841 aagggttcct gctagcgaaa gaatacttcg gttactatta tattgaagcg ttcctaacca
      901 gatacacaaa aatacaacca caaaagtgat cagcggaatc agccaggtgc tattgcgaat
      961 aataaagcag ttaataagct tcatcgcgat tatttataaa gacaacatgg ggtagatgtc
     1021 tataacaact tcttggtagg gatgaacatt cttaattacc tggataattt cgttcacttg
     1081 gtctttctcg caggcaactt caatccgttc ttcgtctacc atttctaatt tccccacttc
     1141 cccaatgtgt ggattagcac catctcctgg cttaaaacgt cccacacctt ttactgagta
     1201 actacagaat gtgtaattcc cgactcgtcc cgctccagct tctcctaatg cctggcgaac
     1261 ctggtctgca ttttcttctg ggacaaatac gactaactta acaagttctt tcatctaaga
     1321 cctttcgata aaactattta atgaattggg tcactattga aaatagtttg gcgtattgcg
     1381 actggctgcc cgctatgaag atccgatcgg taggattaag gttcgaggaa ccttcacgat
     1441 ttgttgcttt accgcctgct tcgttcacaa gaaaagaccc tgcaaagttt ggaaatgaat
     1501 ctttggcaaa gcagataacc ccactcactc ttccacgcag cagatcacaa taatgaatag
     1561 caactgaatc agtatttgta gccaattcgt aatttgctaa agcatcttcc agttctttta
     1621 atgaatcagt ctctcgccat tggcttgaaa tacgaaaaat aaccttttta ttctctactt
     1681 tgttatctat atttaccgaa actccattca taaatgaacc cttacctcga taggcggtaa
     1741 atagtttatc tatagaggga tcgtaaacag cggcaaactg tgcctgacca tctagaatat
     1801 ggctgacaac aatcgcgtaa tgcggccgac cttctataaa atttttcgta ctgctaatcg
     1861 gatcaataac ccaaacattt gatgtagctt gaaaattatc gttttcttcc tctgcaaaaa
     1921 ctgagtgatc gtcacctaac ttagtaataa tgtccgttaa gccacgttca atccgtagat
     1981 cctcctccgt aagatattgt ttggtaattc caatatcttt aaccttgcct gattcttctt
     2041 ttaaacgttt accagcgtcc gtaatgaatt cgataatttc cgagtacgta gattccatcg
     2101 ctatacctcg ctctttagca ttgctttgag gatctccatc tgttcgggag agacctcgat
     2161 tggttttaat tcagttttca tcggccaatc tgcatctgtg tattcctgat taccgagtgc
     2221 cacgggttga gagtaacgtg gaataaagtg aaagtgaaca ttggggtcca ccatcatcaa
     2281 agctaagtag ttaaactttt ctgctccaaa tgtatttctt aaaagttcct ccgtttcctt
     2341 agttactaaa gaaaattccg tccatgcatc ggaagaaagc tttcctaaat ttgtgacatc
     2401 ggacttggca gcaataacca acgatccaat ggttacctgc tgaggccgta agagaactac
     2461 ccaatggtca tattctttaa ttaatgattc aggataccca aaagcttgga ctgtttggtt
     2521 catagcatct ccttcgtttt taaattgact tttacccgga ctttttctgt cattgttttc
     2581 tggctcacat aagcaagcac gtcactgtca ggatcataca gttctatcat atccaaatca
     2641 cctcgaggaa aaatacgggc gcccttacgt tcttttccat ccaagagtga ttgttttaaa
     2701 tccgtatact gatttgactc tacataaaaa tctacatcgc ctacatgatg ccgatatgga
     2761 tctggtttcc gaatataaag atacgtgtac gttgcttctg gtatttcacc atcagcgggt
     2821 ataataattg gcttgcgaag cctaaaatat ttttgatcta cgttatctga cggctcggtc
     2881 atttcttcac gcagttttac taaatagctg tactcatcgt cgtaatgaca aaaaacaccc
     2941 atatttccag cgttgggcag atagtatcca aacgactgtt tacagagttt ataacaagat
     3001 tcgtgaatat atttaatcgc gtctagcaac tgttctttgt ttccaatagg actaaatcga
     3061 tagagcttca tattgattac tccattccat acagtagttt acgaaactgc tctctttggg
     3121 atgggtcata aagtgcacgc agagcaaatc ggccaagcat tccttctttg tcgtgaaagt
     3181 catctcgtgc cattatctct actaggccat caaaagagat tggtttgaat tgtatcctct
     3241 ctcctgaatc aagagactga gagccaaaag ggcggcaacc tcgtgcaacg tacgtataaa
     3301 cagaccattc aattttggaa acaggctgca ccgcatcaat aagctcccat tcagaagctt
     3361 cataacccgt ttcttctctt aactcacgtt ttgcgccctc aagaggatct tctcctttat
     3421 caatccgacc gcctgcgaaa ccaataaacg gaggtttacc aggctgttct tgctcgagca
     3481 ataaaatatt gccatcttct aatatcggaa ttatgagagc tgtatcgtct cgcttgagct
     3541 tttcaaatat tttaacggtg ccatcaaact gtagttgctc ccattggtaa acatcaaaaa
     3601 taatcccatt aaaaactttc ttagcatttt caggtaacgg ttgtttcgat agaggtttgt
     3661 tcatacgtat cctaattgtt taagaataac atcctcaacc tcaaccattc ctgttgagag
     3721 agcttcttgg taagccatat gtgttcgatc acctggcaac cgcattcgtg atccactttt
     3781 tgttcttgct tgtcgcattt tgcgcactaa gtcggaacag tttgttttaa attctgctgt
     3841 gtcgacaagt aagtttggat caatggcgat gaacaaagta ccccactcgc cctcaagttg
     3901 accatacgcg gctttcacca gcgggcccgc cataacttca acaaccattc caattccgga
     3961 tcccttataa cttcgatcaa aaggcaataa agctccatcc attgccgcac caggatctgt
     4021 tgtaggttta ccttcggagt caattgccat atgttctggg atactttcac cccgtgcctt
     4081 ggcaagaatt aagccataaa atgtcatcgc agaggtagcc atatcgaaga cgaccggctc
     4141 ttcgttcgta gggaacgcaa accctattgg attcgtgcca aaaagcggat caatgctatc
     4201 aaagggggcc gttgaggcag gagatcgaga catcataatc ccaattaagt cttcctttgc
     4261 tattctctca acgtaaaatg cctgtgaacc attactcgaa aatgtgttgt gtacgccaac
     4321 tattccaaag ccatgttctt tcgccttggc gataaccgcg tccgtagcaa cttgactcac
     4381 caatggcgcc ggatgagcac cagcattaat aagctgggaa agtttggtgt ctcgttcaac
     4441 tctcatctca tgctgaggcg taatattctg gagtggttcc ttacccgtca ttttaataat
     4501 gccttgagtc ttcaccccgg ccatttccgc ccatattaaa tactctgcga ttcgttcgga
     4561 ctcttctttc gtaaattttt tctgcagagt tgtttcaact ttatgccgta gtatatcaat
     4621 tgcaattttc atacctagaa acctttcttt gaaagtcgta ctaaggcttt tttcatatcc
     4681 ccactaaata aaaccaagtc tttcaccggt tcaaagccta aggatttata ccaacgtaaa
     4741 ggaaactcat ggtctgcaaa agtaagagct tcccacacgt cggcatcata tttctcaatt
     4801 gccgtctcaa acattacttt taataagcga gatcccgctc cgcgtgcctg ataatctgga
     4861 tggacaaaaa tctcgccatc aactaaatgg ttgccattcc accacggctt aataccagcg
     4921 ataaaagcac caataacttt tgaatcatcc tcagcaacaa aagctaaatc tggttggtgt
     4981 ttaaaccaat acgccaaaag atcacgcgct gaatctttct cccaccgctc ccccacatca
     5041 aatactcggt aagcttcgca gtaaacttcg gccaatatgt ctaagtcgga ttcgttcatt
     5101 aaacgtatat ttatttccat gcgtcgagta taccaaaaaa gaaagtattt tacttactcc
     5161 tcttttcatt gaccatcttt tattaactca cttcgcctac aaaagactcg gtaaaacggt
     5221 cacgtacttg cgacgcgttg tttccgtttc caagtaaata catgagcttt atttgagccg
     5281 ccacatcagt catatcccga ctcgaaatag caccagcttc aagagcccgt accgcaggtt
     5341 catcattcgt catctttgaa ctattaccac ccaaaaactt ggtggtaata atgacaacaa
     5401 tgtcttgctt caccatgctt tcaataaatg gaagaaacga tcctctccct tggtctggga
     5461 cactgccagc cccgtgtgat ttaagaacaa tccctttaat ctctcctcgt tctaccagcg
     5521 aagtcagcag cataggatct aatccgggga caatatcaat acaaatgatt gagctactaa
     5581 actgtggcga acgacgctca aatattccgc cttgtcgttt ccttacgaga cctgaaaagc
     5641 taataccgtt tgccgtaact tgtgccaaag gtggaaacgc tggactagaa aaagctgcga
     5701 agttagattc actcactttt accgttcgac atcctcgtaa tactacatcg tgaaatgaaa
     5761 ccatcacttc acatatgtgc tgatcaatgg ctaaacagca cgcgcgaaca ctatgctcta
     5821 agttaaagtg tgcatcatca ccgtacacac ttaaaggtaa ttgagaccct gtcagtacca
     5881 ccggtttgtt caaagttgac cctaaactga aggctaaggc tgaagctgtg tatgccattg
     5941 tattcgtccc atgcgcaaca acaaatccgt catactgatc atacttttca gtaatgcgct
     6001 ggtgcaggag catccactca tcaatcccta cgtttgtgct atcaagattt accacgactt
     6061 caaaatctaa ctctgcaata tcgtgcaaac ggggaacttg attcatgagt tcttgaatgt
     6121 agtgagcagt ccgaaccaca ttattttcga cgatagaaaa aatcgttccg cccatggata
     6181 ataaaagaat cttcggtttc atatactccg cactatatca aaaaaattca tactccttaa
     6241 aaatgatcag gcaagagtcc ttgcgtaacg caatctcgga aactccaatg tggcatatcc
     6301 tctgttttat acgtccaaaa gaaccacccc tgtgctttct caaaaacttc gagctgcgct
     6361 tttccgtaac ttttaaaagc ttcacccttt tcttggtcgg agagcccctt gagagataaa
     6421 ggatccaatc ctaaactcca ttcaccaacc actacaggaa ctttttcttg tatctgagaa
     6481 atcaggcgtc cccattcacc atacgttttt tcaaggtgcc ccataaaatt aagtgcttta
     6541 tcttcgtccg aaaaacactg gtacatatgc atgtctaata ccacatcccc catagaagaa
     6601 tttgtcataa aagggatcca ggcaggagga tcaaacgcat catggatgac aatcttgttt
     6661 tgatatcctt ctctcctcag cgtttcatat gcatgacgat aatacgtttc caaaattggt
     6721 ttttgaattg tccagtgggg ctcattgagc aattcaatac ctataacgtg cggataactt
     6781 tggtatctct cgccaagccg tttcagaaca aaaagacttt gatctctgtg ggcaacatcg
     6841 tttggccaat taatcgcacc tttttgacca ctatggtccc agccattctg gctgcctgac
     6901 gcagtgtgta catctataat gaccgacaat ccgtaatctt tagccgcttc aatggcaaaa
     6961 tcgagatatt caattgcacc aaggtaaggc ggagcgtcgc caaaaattcc atgaccgatt
     7021 ggtatacgaa cagcgtttaa tccacgttcg gcaatccagg caaagtcatc acgtgtaata
     7081 taactctgcc gatgacgatc cactacttcg aaagcccgat ttcccagctc tcgacagaaa
     7141 gaccattcgt cttccgcggt atagcctgaa aaaaggctgg gggtaatcca tttttccagt
     7201 accagccatc ccccaagatt cactcctcga aatatatttt tcatacggtt actatattaa
     7261 tttatatcgt aagcccaaaa tcacatctct tttcgttgca tccgcttatt tataagtggc
     7321 gaaaagtggt ctttatagtg tttttgtagt tctggacgcc gccaatcttt acccgtgatt
     7381 ttgtgccgac agcgtgaact tccgcattta cattcccacc gggacacata gttttcatcc
     7441 gcctcccaga gtgcgtaatc agccgttaat tcctcgcctg gattaatgtc ccgacgtgcg
     7501 ataagggtgt acgcgtcttt catccagaga ttcggctcgc atgagtgatt aatgaagtaa
     7561 gcgtcatcat ccccccggtc ttcaatacta aaaaggttgt cgtcccactg catcacgagt
     7621 ttgcctgtat ttctcgcgac ttctgcaccg actctgtcgg tatactctcc tccccaacgg
     7681 accacttctt cgcctccttt aattaattcc ctcgcaaata agccttttcc ttgaaccggc
     7741 gagactttta tctctgcttt tgagttaatc caagaagagt tattagcaaa atttttgaga
     7801 acccaagcct caccatcctc aggaaattcg tatctaatga cttgttccga gttttcaggt
     7861 ttctcaaaat gatcaattgt ccactcgact agctctttgg ctatatgcga tctggttttt
     7921 agattttgat tttcttccca acgtttccaa atcacattta ccggtacatc aatgaaaata
     7981 acctttgcat ccgcacccac tcctgcagca tacgtccgga gcttttcccg gctatcaaaa
     8041 tcatgatttg tactatcaaa aacaaccgat tggccacgcc ccagtttatt tctcatatct
     8101 tgatatgacg ccttaaaaat ctgtcgccaa ttatcggcag ttaccttatg gttttcttcc
     8161 caactgaaac cgagttgatc gcgaatcaca tcaatactga cgtaagcaca gcctaatttt
     8221 ctaataagag atttggcaag agttgttttt cctgaatatg gcaatccaca cagtatataa
     8281 agaattggtt tatccatgcc tgtactatac caaaaaataa ccgtcacgtt gccagtctgt
     8341 cacaaaggat cttagaatgt ctcttacttc aaatagttgt tttgcaaatc ttgtgaggta
     8401 acttttagga tggtggcatc atctccatct gcaatagttg caaaaattgc ttttgcatca
     8461 gcaatttttc tctcttctaa cttttctaac tcatgttcct tgtcaggcgg aaatcgaaag
     8521 tcagttaaga cataatcaca cttgtaggct gaaccaaaaa ctttttgcat gttaaggaat
     8581 gctctttcat taatctcttt atattacaaa aattgcttac cgtagtaacc gactctttct
     8641 cgattatgta ctgggatgcc tctaaaccaa ggcttttccc atactgtgcc attgcttctg
     8701 cttcagtcag gggtggagtg taagctaaac catatgtcca tttgccacta aaaataatgt
     8761 gtccaaccgc cccgtcggta agcagatccg ctgctttttg cgtgcgaatt ttagcactct
     8821 cgggtatagc cccatcaggc ttaatcccgc tgccaaggac cagtgcgaca tcataatggt
     8881 tattaagttt attcgtcata tctgaactat accaaaaaaa cagccctaca aatgcttata
     8941 taaagtttgt tgatataaaa ataagaaata aggtaaaata tatctatgaa taaagaagaa
     9001 ataatcggtg gtaccgtcca cgaactacca caagatttaa aagatgccct tctcgctacc
     9061 ccggccgccc gtaccgcatg ggagaaccta actccgctcg cacggaatga gtggatttgt
     9121 tggaatatca ctgttaaaca acccgagaca agaaagcatc atatagaacg aactattgcc
     9181 gagttacagg aagggaagcg ccgaccttgt tgctggatcg gttgtattca tctccctgac
     9241 aaacaaataa gcccatccgt ccaatgggta ctcaataaac aatcgaaatc aaagtaactc
     9301 gagtataaag cctaagatac tcttctggtt tgcagacggt aacagaatac gagctgacat
     9361 atcggttatc tgatatactt taggcatgac acccgaattc aataaaactg acacatctga
     9421 actagatcct gaagcaatag aagccgcgga gattcaagcc ctactgccca ctgctgctca
     9481 tttttttggg ttgggttcta ttcaaaacat taagcctacc cctttcggta ttggcaacga
     9541 aagtttcttt gtctcgagcg aacagggaga ctttgttcta cgggtgttat gggagcagac
     9601 tattgaaggc ctagaaaaag aagttgccat tgagcaacaa ctcgcacacg caggtgttct
     9661 cagcgctcaa cttttacaag gtgagaatgg caattactat tttaccgacg ggaaatacac
     9721 aataacagcg agccgaaaaa tcgaaggaat tcaccctcat gaaataactc ctgatcttct
     9781 caaccaggta ggaacaacca tggcaacatt ccatacgagt gtgactgaaa ttcctcagga
     9841 aacaagtcag tggtttacgt tagataacgc ccgcatcgaa gtagcgaaag tatcgagtga
     9901 cgaagaatca aaatctgcta ttgaaaacaa cgttactacg ggatcatcga tcttcaatcg
     9961 agacctaccc acgggtttta ttcatggcga tctctacaat ggtaacctta ttctcacccc
    10021 agataatcgt ttagcggtgc tcgactttga aacaaccgag cgcaacgtct taattattga
    10081 tatagctcgt actattttag atatgtgtgc cgacgacgta accgtgaata cacagtcagt
    10141 tgaagcttta ttggatgggt atgaagaaat acgccagctt accgatgaag aaagggaaag
    10201 tctctccgat gcaattcgat atgtaagtgg cgccatggcc gcatggctca tagttgagag
    10261 agagcagaaa gaagttgcgc aaaatattct tcagcgagct gaatcattac gttcataagt
    10321 aagactacgt aaagtcagaa cccttcccta tcgtcataaa atattgaata taataacatc
    10381 actatgatta gcggaagata ttaaagttat ttgctaaaca atccttcgat gaattttgat
    10441 ttggctttac ggtagtcatc catggactta ccatcatatt ctcgctttaa ctgattaaat
    10501 gactccctcg cagagacatc ttcatttaag atgtctctca atttgtaaaa atagctatcc
    10561 tcttcagagt ttattattgt tagctgtaat ccaacagcag tctcaccact atcatcttta
    10621 aaactagcaa aggtttttgt ccaattttcc tcttgagcag gttcgtattc tttactcaaa
    10681 acctccttag tcggctcaaa gtcatctcta cttactcgta tctgaatatc taaatcacct
    10741 ttagatatta accctattac actcgtagcg ccaacatgtt gaatgtccac agactttcct
    10801 actagtgacc tactaacttt ttgtttatga gattgaaata aattctcaat attctttaaa
    10861 tctaaatttt taataataaa atcatccata gccgcactat accaaaaaaa cagccgtata
    10921 gctgcttcct tggttgacag tcggtcatag acgatgttcg aacctatttt cttactctct
    10981 ttccatatac ttaacgagct ccatgccggt ctctactttt tcaaaaccaa atttctcata
    11041 aaattcgctc gcgtctttat tctcttccca tacaaataaa ccaatagaaa cataactttt
    11101 atcttttact tgctcaatga gcaagtccat aagcttccta ccaatttttt gattctggta
    11161 atttggcctg accccaatat catagaacat gcacattacc ccatcttcaa gtattctacc
    11221 cataccaaca agcagatcac catcccatgc actcacaaca aacgaagaag cagcgagtac
    11281 acctttccac ttctctttta ctcgacttcc ccacccgata gattcaaata ggctattcag
    11341 gtcgtgagca gaaatatctt ttactgtctt gaattcaatc atatggttcg attataccaa
    11401 aaaagcaacc gtacagctgc ctctttgttt ggcagggcat ccttatacgg gcttattttc
    11461 caaatctacg ttcgcggtcg gcgtattcgg tgaggatgtt ttggaagtct tgtttattaa
    11521 agtcggggag aagcttttta acgaagaacc attcgctgta gcctgactgc cacgaaagaa
    11581 agttactcgt acgatgctct ccacccgtac gaataatcag atcaggttca gactgtaact
    11641 gaagctgcga ttcaaaactc tcgagcgtaa tatctttgag gtcgatagct ttttcgagaa
    11701 cttttttaat gagcatcttg gcggcactaa aaatctcttc cttgccaccg taggcaatac
    11761 agaggttaag ggtaaagttt gaataattct tagttgcctc ttcggcccga cggattgctg
    11821 cttggacaga ctggggaagc atttcgacat gtccaataac ctttatccga attccctgct
    11881 tatggatacg ttcgtcgtca ataaattcat ccacactttt ttcgataagt tccataatct
    11941 tgtcgacttc ttctttgggg cgaccaaagt tttgcattga gaacgtgtaa agcgtaagct
    12001 ctttcacacc aaactcgccg caccagtcga gaagctgttg gacttttttc ttaccaaatt
    12061 cgtgaccgcg ccatgggcgt aagccacgaa gtttggcaaa acggcgatta ccatcgagaa
    12121 taaaaccaat gtggtcaggg acaatgagcg taggcataga gataagatcc ttcgtttgtc
    12181 tcgaatattc taccatattt ttgagactct tttaaccctt accaaccaga gcaatcacaa
    12241 gtcccactac cgatccaata gcggaaataa tccagaaccg ctcggttact tttgtttctg
    12301 gccagccgag agcttcgaag tggtgatgaa taggagccga acggaatatt tttttaccac
    12361 cacggagttt cttggatcca atttgcagta tggtactgac tccttcgatc ataagaataa
    12421 tgccaatgac tggtagaaca aagacactat tggtaagaaa cgcaatgacg gctaatgtca
    12481 tcccaagtgg cataataccg gtgtcgccca taaaaaatcg ggctggatag atattgaacc
    12541 aaagaaacgc aagtgttgaa ccgacaatgg tgagacacaa cgcggcaagc tcgactttcc
    12601 cctgaaaaag agcaatgaag gcaaaagcgc tgaaggcaat cgccatgacc ccaccagcca
    12661 aaccgtctaa accatctgtt tgattggccg aaaaggctgt ccagactaac acagcaacga
    12721 agaatggcat gtaccaaatt cctaaatcga cattgccata gcccggaata tggaagaaac
    12781 taaacccgag ttttgagtaa aaccaccaag cacccacaac cgccacaatc gtatagagta
    12841 agaaacgaac ccgaaactgc atcccgccgt tatgtggccc aataccgtag atattcatca
    12901 ggtcgtcagc ggcgccaata agaccggttg caatcaatac aaaaagaggc aaccaagtcc
    12961 cagcccgatc caggttgagc aggagggtaa taacgccggc agtaatccat attagcaatc
    13021 cacccatcgt tggggtgttc tctttgccct tatggagact gtaaaaaata ggtgctttct
    13081 tatcgtcaaa tgccgtctga cgaatctttt ttcccaaacg attcttaaac aaaaaatctg
    13141 taaagatggg agtccagagc atagcgatga caaaactaag cgttcccaat aaaaacacaa
    13201 acttaagatc gcttagatta attgagaaaa cttccataac cctaccttac tttaaattta
    13261 tggccgatga ttccactcag gaccttggcg gacgagtaat ttcgaagctt gctccgggga
    13321 gcgcatgagc tctttaacca acttctcaag tcggatcgta ttctgtgagc ccttaactaa
    13381 cactataaca tccggcccga gctgcctcaa cgcagaagca acagcttcag gcgatgtcat
    13441 aaaccattca tctggcttat acaatttgct gagtggccct actccaataa cgtagtccgc
    13501 acactgccgt gcgaatgccc ctatctctct atggatgggg tctggagtga tgagctcacg
    13561 catatctcct aatatagcta cccgtttact ggcaggaagt tggcgtagaa gctccaaact
    13621 gccccgcatg gcaaggggat ccgcattata gctttcatcg ataattatac tttgacgctt
    13681 tccttcgatt aacattcctc ggccaggaag aggacgaatt tctttaaacg tttgtaattg
    13741 cacagcggga gaaatcccta agttatctcc aagagccgcc gcgaacataa ccccatacat
    13801 atgttgctcc ccataccttg caatatgaat tcgctgggtg cggttgtgat ggtgtagcac
    13861 ggcttgcgtc ccttgtaagg tcaccttcac acctgaaaca aaataatctg ctcgtcgttc
    13921 aatacgaatt aagatttttt cttgggtcgg ctgaatcatt accttattca aagaatcatc
    13981 atcaccattc aagataatca cgcctttagg atcaacgaaa tgcaaaaggg ataatttctc
    14041 tttttttagc tcctctaacg agccaaagta agcactatga acctgggaaa tattcgtgat
    14101 aattgcatga gttggacgga ttaagcggga aaagcgtgca atatctcctt tgtgttcggc
    14161 gccgacctca ataaccatac attcgggtac ttttcctaaa aaggcagtca tcgcgccttg
    14221 taatggcgcc atacaccacg ctataagccc acgtggctct tctttaaatc ctaacaccgt
    14281 tagaggaacg ccgatttctg cattcaagct tttaggggtg acaaatacat tttccccata
    14341 ggccttacga actaatttcc ctaggatttc cttcgtgctt gtttttccgg cgctgcctgt
    14401 aatagcgata acaggccctt ggtaacgatt acgaaaaatg ctagcttccc aagcaagtat
    14461 tcctgtatac atccgaaata aaatagtttt gaccatacaa atgtttagtt tagcagcctt
    14521 acccttaatt accaatagtt tcgcacataa cctaccctta cctaaccaga gaacctcccg
    14581 agcgtcaact gaaaaaaact gtataatatg tacgcttcgc ctattgcatt ggcttatcgg
    14641 gtaaaatttg cttatacgac agaatccaat ttgcaattct tccaaaggtt ggcgccgccg
    14701 aggactcggc gaattttgtt gcttttgggt tgtcgatttt tacgaccatc acaaattgtg
    14761 gatccgtaag tggaaaatag cctgcaaacg aaccaatatg acggtcgtca tagtaaccac
    14821 cctctggctt tacaacttga gctgtccccg tttttccacc cacacgatat cccggaacag
    14881 cagcatgttt gccgtgtccg ttgacgacca ccgcctcgag catgcgaccg agcttttcgc
    14941 tcgttgtttc actgacgacc cgccctaact cttttggttg tgtttcggtt aatacacctt
    15001 tttcgtcgat tactttttcc accagatacg gctgcatcat gacgccttta tttgcgagcg
    15061 cactataggc aatcgccatt tgaagcggtg tcgtcgacac accttgtcca aatgagatcg
    15121 tagcgcgggt aagatcattc caatttttca gccccggcat gaacccgccc accacgttcg
    15181 aaagattcat atcgggcgcc gtaccaattc caaactgttt caaaccatca tattctccat
    15241 tattaccaag cttattggca atccatacca taccgacgtt gtcagagttt tcgaggactt
    15301 gcgtcattgt ctcgagacca aaagcttttt tctcggctgt ccaaattgta tggtttaaaa
    15361 ccttaaccga agcagcaaat gtctcctgcg tttccggctc aacaagcccc ttatcaacag
    15421 ccagagccat cacaatcggc ttcataatag atccaggctc ccaaacagat gaaagtgaag
    15481 gattttgata cacggccgat tggtcggagg ccactttatt aaactcgttc gggttatatg
    15541 tgggagcatt cgccaaggcc acaattcccc cagtttttgg gttcataatg acaacactgc
    15601 cactatcggc ttggtattct ttgatcgcat cagcaagggc ctgttccgca tagtgttgaa
    15661 tctctcggtc aatcgataaa actaccgttg aaccatcttg cgcctttact tcatcaccca
    15721 cattaattaa tcgtccgtga ctatcttttt caccagtctg gtagccgctc gcaccgcgta
    15781 aggtcaaatc taatgcccct tcaatcccat aatttccctt tccctcccca ttaacgaacc
    15841 caagcacttg tgccgcaagg gtttgttcgg gatacacacg caccaactca ggcaaaagaa
    15901 caacgcctcg taagcgcagt tgggcaatcc tatcggcgac ctctcgagtg agtcgcttct
    15961 ttaaaggagg gatatacaat ttatcgttat taattttgtc gtacacttct tgttcggtca
    16021 ttccgataag tgaagccagt ttttcggccg tttgtttacg gtcctttata tttttcggca
    16081 cgaccagtac ttgataccga cgttcgttcg tcgccaaagg aaagtagctc ttatcatcct
    16141 gagcaacaac aatctcaccc cgttggcctt caacttcttt gcgataggcg tactgacttt
    16201 ccgcttcttc aaccgtttgc ccatgttcaa taaccgtctt ttgatagaga cggccggtaa
    16261 agctcaacag aataagcccc cccactgaaa gaaggatact aaggcgtttt tgccactcga
    16321 gaaaatccat aggaccttta gggttaccca cgtgtaaggc gacagcgtcg gccgcacatt
    16381 ctagttactt ccggcaggag aaagaaactc tactgactga actggttcga gttggagtgt
    16441 tgttgccgat tgagaaatag tatcgagaga ttgtaaacgt ttggcttcta agcgaagttg
    16501 atcttcttcg gtagtaagca agtcagcttg ttgacgggta gactgaacat cgagccgctt
    16561 agtagtagct tggcttgact gtgcaatata taaaagggct aaaatagcga gcataagaaa
    16621 tccgatcatg cggaccgaaa agaaaccaat acgagccgcg gcacgtcctg gacgatttat
    16681 acgagaatta actgtcgttg ctccatacgt taccggaatc atactgtccc ctccattatt
    16741 tcttataagc taagcgtaat tttgcactcc gcgcgcgcga attctttttg acttcggtaa
    16801 acgcgggttg aataatcttt ttggatactt cgagtagctc acgctgactg agcaaaaagc
    16861 gtttcacaat ccgatcttcc cctgagtgga aactaatgat tgcaaggact cctccgggct
    16921 tcagcaaacg gattgctgcg ggaagcgctt tttccagttg ctcatattca cggttcacgt
    16981 gaatgcgaag cgcttggaaa atcggtgcga gttccatagg ccgccagagg ttaagggcct
    17041 ctttaaggtc atgagctact tgaaatacct gttttcttcg gtaatttaca attttgtggg
    17101 caagagtccg actattatga cggtcgccat aaaggtaaag gacatccgca atttcgcgtt
    17161 catttcctcg ggtaaggaaa tcggcggccg acatatcaag ttcaggtgat aaccgcatat
    17221 caagcggcgt atcgtcgata atgtttaagc catattttgg gtcatcgagt tgccgggaag
    17281 agattcctaa gtcaaacaga attccatcga ctggagaaaa tccctgtgcc tcagcgacat
    17341 gttgcatatc cccaaaacga ctttgaacaa gaactgcacg agttccaaac tgcgtaaggc
    17401 gctcacgcgc gattgccagt tggccaggat cttgatctaa gcctaaaacg cggcctgttg
    17461 gcccacttgc ttctaaaagt gcttcggtgt gccctccgtc acctaacgtg gcatctatat
    17521 agttgccacc gtctcgtggt tggagcgcaa ccataacttc attcatgaga acgggaatgt
    17581 gtttcatacc ttaatctttc ttctctcgtt acgtaacact ccctcccatc ctccctataa
    17641 cccatggcca gtagcaggta tgtgcccctc caagattagc tgatccctaa agaagtttct
    17701 ttatacggga aggactaacc ccggcgtgta taaggacctt acgaataata ataacaatat
    17761 cgtttgatca cgggtgttac ttgttaccaa gtaacgatgc ccactactgg tcgatgagtt
    17821 tttggtgccc ctcgcggcct ttagagccaa ggagggcata caaagagtgt aaggtactaa
    17881 attcccagat cagagaggcg gcttgccaca tctgacgagt tcgcctgtgc gtcagcactg
    17941 taatccgacc aacgggcttt atcccatatt tcaactcggt tatacagacc tgcgacgatc
    18001 acatttgcgc caagttcagc atattggcgc agataggcgg gcaagatgac tcttccctgc
    18061 ttatctgctt caacatccac tgcaccagcg agaataaaac gagcaaacga acgtgcattg
    18121 gcatctgtcg taggcaatga tgccacacgc tccgcaagac gttcccattc ttttttaggg
    18181 tacaaggtca aacaattatc taatccgcga gctataacag caccttcccc tagggcagta
    18241 cggaatttag caggaattgc gagtcggcct ttctgatcga tggtgtgttc atattctcct
    18301 agaaacatcg atcttccttc cgcttcgcct cacttcgtgg tgaatggtgg ggtagttgtt
    18361 gagggacatc tgcgaacgcc taagcgttcc cgatgcgact gccccactat tcaccaccca
    18421 tccccacctt tccccattgg tcttaaatta cgcgttctgc gtagagctgt caagccactg
    18481 tctatcgtgg aggcgagtgc cctcactaca atatgtagta tgcccagacg tctggagcca
    18541 tagtaccaca agatacaagc cctttcccca cttttatatt gactcccaac atgtggtagc
    18601 ataaagacat gcaacattac accccactta aacttctgta tgtcgccttt ttaggcctca
    18661 tgctcgccct attcaccggc gtccttattt cggtcgccta tccgggacca aaagcgcccg
    18721 aagatacccg tacggttccg accgtaaaaa ccctccctga aactggcacc ccagctactc
    18781 catcccccga agagattgcc caacaaaaag agtacgatca aaagtggaag gacttccaag
    18841 accgtcaaaa gacttatgag cgcaatgttt cattgattgc attgggagca tcagttatca
    18901 tgctcgtcgt cggattagtc ttagcgacaa aaatcgacat ccttgccgac ggctttctcc
    18961 tcggcagcat tttcactatg ctttacagca ttattcgcgg ctttaatggc gagagtgagt
    19021 ggcaacgcct catagtcacc accgccggac tcgtcctggc gcttgtactg ggttacgtaa
    19081 aatttattaa accgagccaa aagtaaaccc tacagcttca aataaggtat aataaaggcg
    19141 cagttcacca ctgcgctttg tgcgtttccg atagcggaac cacttaccag gagagaaaaa
    19201 tgggcagcca gtccttggct caaactgtgt tcgaggacgc tgtcttcgaa cattaccgtg
    19261 gctacttacg gcgaaacgac ccacaaaagc tccgtagtca aagtcgaaca gacgactctc
    19321 ggggcatcgg agatatcctc aaaagatacc cggcgacggc acctctgagc gatgagctcg
    19381 cagccgtcat tcgaggattt cgtgacgtcg agtgcctact ggcagactac agcagcgaca
    19441 tcgcccgcat tgttcggcac gaaccagccc aagtccagtt cacgatgttc tggaccggag
    19501 aagaggccat ccatggcttc gcgctcgacg aagtgttgat tgcgtatggc gccaacactc
    19561 ctcgggaagt ccgtgacgac aaagccagac tactcaccac gtgcaagtgg gacccttcca
    19621 aggtcgacgt acgctttctc gacccttggt atggccctat gtgttacgtg acacttcaag
    19681 aaaaagtaac tggcaacggg taccgatggc tacgcagaag cgccgagcga gaaggccaca
    19741 tcggccttgc aaagatctgc cagagattag cagaagagga gtttaggcat cacggattct
    19801 actacggcat ggcgaaacta gcactgtcct accagcccga caagctaaaa agacagatga
    19861 ctgaagtcgt aactgacttt gctatgcccg gccgctacgc gattcccttc cagcaggctg
    19921 aaaccgcaac gccagacaat ccgctcccgt cagcacttcc tgcttacgac gagtggacaa
    19981 agacggcaca gcgactgggt atcgtcccgg atcccctgtt cgcggtaccg cttgcggcgc
    20041 gcttgcggcg caaatctgaa gtactgcgcc tagacgaaca caccatcgaa ggtcagcgtg
    20101 tcgcttgctg gaacgaacac gtcgcatccg tgctacagat cttgaagggg ctcggcattg
    20161 agctccctcg ggagctccgc taccttgccc ctatccccgt ggcgtaagcc acaaaaaagt
    20221 ccccggcgat tttccgccgg ggcacttctt ttttagttag ccgtttagct taaagtaaat
    20281 cagctgagaa agctcatcga gcttaatacg ctcttgctcc atcgtgtcac gatcacgcag
    20341 ggtaacagtt ccatcttcga gcgtttggtg atccactgtt acgcaccatg gcgttccgac
    20401 ttcatcctga cgacggtaac ggcgcccaat cgaaccagat tcatcgtaat aggtcgtgac
    20461 gtgtttacgt aggtcggcag cgagctcttt tgctttttca gccaagccgt cttttttaac
    20521 caatggcaat acggcgactt tatacggtgc aaaaattggt ggtaagtgga gcacaacttc
    20581 tggttctgcc agagtctctc cttcttcacc ttttcgaccg ttcggatact ctttatagct
    20641 ctcgcacaaa atcgccacaa ataagcggtc gatcccctgc gtaggctcga tcacgtatgg
    20701 cacaaacttt tctttcgttt ctgggtcaaa gaagcttaaa tccttgcctg agtattcagc
    20761 gtgacgctta agatcgtagt cggtacgatt ggccaccgca ccgagttcgt cccagccaaa
    20821 cggaaacttg tattcaaagt caatcgtccc cttggaatag tgtgccagat cttctttagc
    20881 cttaacgtca cgacggatat gttcgcgggg aataccacat gcttcgtaaa aggcttccca
    20941 acgctctacc caagccataa aatgctcttc atccgtacct ggctttacaa agtattctaa
    21001 ttcagcgatt tcgaattcac gtaaacggaa gagccagtta ccaagcccaa tttcgttccg
    21061 gaacgttttc ccaatttgag caatgccaaa aggaatcttc gtacggcttg tttcggtaac
    21121 atatttaaaa tccacaaaca tgccttgagc cgtttctggg cgtaggtaag cagcgctggc
    21181 agattcctcg gtcgcaccaa gatacgtttt aagcatgagg ttaaattgcc ggggctcggc
    21241 aaacgtgtga tctgcacctt cagggcactt ccagaattta ggattatgtt ctttagtcgg
    21301 aacatgatct tctcgaaacc ggaaatggca tttcgtacac tctactaacg gatcagtaaa
    21361 cgtttcggtg tggccagagg catcccaaac gcgtggatgc ataataatcg atgactcaat
    21421 tcccaccatg tcatcgcgac cgtgaacaaa tgttttccac caaaactgtt taatattatt
    21481 gcgtaattcc acaccaagtg ggccgtaatc aaaaagagat gctacccctc cgtaaatttc
    21541 gcttgattga acaacaaaac ccttgcgctt agtgagatta acgataggtt cgagagagtg
    21601 taaagacatg agcacctttg cattaaaaaa taataacgaa atcccgagtt gagggatatg
    21661 gtaagtatac ctaataaggg aaggtttctc aaacaaaaaa ccggaggaca ggcgcatacc
    21721 gcgcgccgtc ctccggtcga gaatgagcat cagacgatcc caaccgccac caggtggcgg
    21781 ccgtcgatac cttccttccc ccaccccacc cagcggtggt gcgagaaaaa gtcagaaccg
    21841 gagtaggtgt caatcggaca ccgctcgata cgttcgcgca gaacgcctgc cgcaacgagc
    21901 tgatcatagg cccgatcaac ccaccgcagg acgatccctc caccagtctc cctgatgtaa
    21961 gggttccatc tcggagagtc gagccgtggg ccacgcgtgt ccatccaggc gccttctcca
    22021 tcagaccctt gccaggacgg ccccatggcc accagaagat ccgacggatt gacgccgaaa
    22081 cggcgctcaa ggtatcgaac cgtcttggtc ggcacatcga ggccaacccc ctggtacccc
    22141 gcatgagcca gcgcaatcgt gcgagtcacc ggatcgacga tgacgagtcc aaggcaatct
    22201 gcgatcgcct gaactagtac cgacggcccc tgcgtgacca gaacatcaca gtcgggcgcc
    22261 aaccacaccc cctcgtgggg caccgttctg atctcatcgg aatgagacat gactggcgcc
    22321 accacatggg gcatctgtgc tgacgggagg tcgaacccga gctcgcgcag gaaagcttcc
    22381 cggcgaggca ggacaacact tcggggctct tgcgacagcg acatgttgcc atgcgccatc
    22441 gtagagcagc cagcccgcag ccactcaaca tcattgaaca ggccaaaggt cagagccctt
    22501 ggctggacct ctatcgcaga cactttcgac tccttcctaa cccgtcaatt tattgccaaa
    22561 cgataccaca aaacacccta aaagtcaagg gtgctagtgg gttaaaatct ggatgcgtga
    22621 acctgttgct tgagcatcaa tacgaaccgt cccaccgaca gggagcgtga gcagaggtac
    22681 cgtatgaccg aagtccaatc cagtaataat aggaatgtgc cgcaggccaa gttggtcaag
    22741 caggaggtgg cggagtaact catccgtcat gctcgagcct cgttgccacc ggccgattag
    22801 aagaccttta atttgagaaa acccaggctg gagcgagagg gcacgaaacc cacgagcaat
    22861 acgtgcaaaa tgctcggcat cgtcatcctc caaaaataaa attgcatctt gaagcgacgg
    22921 aaaaaactta ctgccttgca gaagattaaa agtaagaaga ttgccacccc atagctgccc
    22981 ttcggcagtt ccttcattaa ggaccactgc ccctgggtta agaaataatt cggtactttt
    23041 gcgagaatca ctccatgctc gtgacgaagg gagtgaatat ggatcagacg taaaggttgc
    23101 gttgtaaaac gagtcaacta cttcatctat tcccgtaaaa gctccaaaag atgcatacac
    23161 gggtcccgca taggttacaa atccacaggc aaccgaaatt gccgtcgtca aagtagtaat
    23221 atccgaaaag ccgcacagta tcttggggtt atttttcact aattcccagt ctatataagg
    23281 cagaagatcg ttggcattat acccccctgt caccggcaag atcgctttaa cttctggatc
    23341 cgcaaaagcg gcatggaaat ctttaagacg cccttcaatt ggactggcat aaaaaccatc
    23401 actcaaacgg gcatgggaag aaaagcgaac acgcagtcca agctgcgaca aaatatgatg
    23461 agcgcgcgct tgctcatctt cggggattct cgataaagag gctgacggtg caataacctc
    23521 tacgagatcg cctgattgaa gccgtggtgg ggagatataa gacataactc tactttgttt
    23581 tcattcgagg gattgctggc gagagccgcg caagaatttc ataatcaatc gtacctacga
    23641 actcagccat ttcttcagcg gttatcgcaa tagtatcctg tttgccaaga aggacaactt
    23701 cgtctcccac ctgaggtcga aataccgccg aagcatcaac cattgtgtga ctcatacaaa
    23761 cttctcccac aattgggcag cgcttgccgc caattaagac aaacccttta ttactgagct
    23821 tacggtcaag gccatcagca tatcctacag gcagcgtggc aatcagctgc gttttatcgc
    23881 tcgtataggt agcattatac ccaactgttt cgcctgcttg aatacgatgg atagccagaa
    23941 tctgtgtttt ccaagtgagc gctggctcgg ttgtaaaatt agggcgtttg gcaccataca
    24001 gcattcgtcc aaaccgaacc ccatcaaata agtaatccat gtaatcatgc tccgtcccga
    24061 gcaacgattc actcgcaaac acatggacat agggaaccgg caaaccttct tgttgtaagt
    24121 cgaattggaa ctgacgcata acctcagcct gacgttcggt cacttcacgg tctgacgcat
    24181 gataaatatg cgtaaacatc ccttctatgt gaaccggact cttttcgtta ctcaaagagc
    24241 gaataacttc agtagcagca ggaatggcca taccgagccg attcataccc gtttctattt
    24301 cgagatgcac taatgcctgt ttaccatgac gttgcgcagc cagttggata tcggagaggt
    24361 gttgcggtgt ggttgccaca aggcgaatct ctcgctgtac taatgttcct atatcgtgtt
    24421 tttcagccgg agtcagcaaa actaccggtg tacgcgggta tttttcggcc aaaaataatg
    24481 catctttgca ctctgttacg acaaaaccat caacaccagc cctccaaaac gcatcactcg
    24541 caagctcgag tccgtgacca taggcgttgg ctttcacaac ggcatacagc gcggttcctt
    24601 cgccaatggc ctcacgaact gcccgaacat tccggatcaa ggcaaaagga ttaacctcaa
    24661 tccacgtgtg catagctttg caaatccttt aaaatacgag tcgtacgaaa atcttgatcg
    24721 agcgagtact gcacatgccg cagcaagaga gactctaccc gcgccgacat atccgagtta
    24781 atacggagct tctctacttg cgaaacaggg gtcttcgcta aatagcgcat gaccttaata
    24841 tcatcaatcg agagtacctg gccagctgcg gcgccaaccg aacaactcac gataccacca
    24901 tgggcttcgc tccataccaa gcgttgatct tcagcgagcg gcgtgccgca atgtgcgcac
    24961 agttgcagtt gtggctcgaa tccaagcaaa cgcgagacct gcaaaataag cgacggcaac
    25021 acaagccgat gatccgacac caccagttgt tcgatgccct cttgaaggca ccgatataac
    25081 ggcatgttcg cttcctgctc ttggcaccct ttgtcgacaa gttcggctaa tatatggagt
    25141 ctctcgtaga gcgtgggatc ttcggctacg ccatcaaaaa tttgttcgtg actcatttga
    25201 gacacaatgg ggatagacgt tttaaagtta agctggcatg agaccaaagt aaaaagttcg
    25261 gtgtatccta aaagtttgct cgtgatacgt cgagcgcctt tggcaatagc acgaactttt
    25321 ccattttttt cgctataaag cgtaagcatt aaatcagctt caccaaatgg aacacgccgc
    25381 aaaataatcg ctcggtcttt aactgtctgc atgatagggc tagtgtacca tataacacgc
    25441 cgcaaaccag acaagaaaga gagctcctag agccgctttc tgttaagaaa agtccccacc
    25501 caacggcatc gcgcgcgtca ggcagggact gggagaaata ggatttcgca gaccatgcta
    25561 ggtcttacgc tgaggtcgac cgaggataac ggcgggtgta gagcggtctt tttaccgacg
    25621 ctccgtcttc accttcggtg accgttccac tgcaga
//
LOCUS       DASWYB010000038         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_644173, whole genome shotgun sequence.
ACCESSION   DASWYB010000038 DASWYB010000000
VERSION     DASWYB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644173"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..502
                     /locus_tag="VGE59_02325"
     CDS             188..502
                     /locus_tag="VGE59_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY1074514.1"
                     /translation="MMDKMKQAKQLFDLQKKAKAMQKELRDTEIEAVSNDGKITAVFN
                     GELKLVDLKIARDYMDNNSLDTLEKSIKTTVTEALSKAQQVAAEKTRDIMKDMNLNIP
                     GL"
     gene            508..1116
                     /gene="pth"
                     /locus_tag="VGE59_02330"
     CDS             508..1116
                     /gene="pth"
                     /locus_tag="VGE59_02330"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY1074515.1"
                     /translation="MKLIVGLGNPGEKYARTRHNLGFMVLDHMAELFGASFCDQSKFK
                     AATAEIHSGGEKVILVKPLTFMNLSGDAVMALANFYKLETGDIWVIADDLDLPLGKVR
                     VRNGGESGGHNGLKDIMAKLGTGDFWRIRVGIRGEEVRELNHQNNITTQDYVLARFAD
                     NESKMLYSTLESVAVLLKESIERNHLVAHTYQANGFDEHIGT"
     gene            1216..1920
                     /locus_tag="VGE59_02335"
     CDS             1216..1920
                     /locus_tag="VGE59_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074516.1"
                     /translation="MSENQKTTRERISKQTLKARLAEKRSENKKHSTRFLNEEAKIAD
                     KTKQALPLEEALGLVKQTASTKFDSSVELHIHLTPKKGKKGVEDELMRGMMHLPQGLG
                     KTRKVVILNEEIIETLAKTQNVDFDIALATPALMPKLGKVAKLLGTRGKMPNPKAGTV
                     TDNPEQVKAEIEAGRVEFRQDATRNIHQMIGKSSWDVEKLADNARAVMKSFPKNRVMS
                     VAVTSTMGPSVSVVAE"
     gene            2056..3141
                     /locus_tag="VGE59_02340"
     CDS             2056..3141
                     /locus_tag="VGE59_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEY1074517.1"
                     /translation="MKWWTLILSIAAVSAMAAQLRAPYGTELGRIDGVVAFSSGDRLW
                     KSPKEGNQYQCVDLVKRFVQSKGVKWVSLDGQAAASLFPMVEAGRVPGLTSFVNGGNE
                     QPQRGDVVCFSDGGLGHMGIVYEVQNDEVRLIEQNWHAQPNLYLGLPLKKTEGMYELE
                     GRTTKPLKGAMVFWRVQGWVRVKGPPRPLFPSYTYVLVDTSVERDSQLFQQSQYLCRQ
                     ILNRLPDEAKFSIILVHETSEQIAIDPVKNWKSDLTYVYRHYVKVKGRRVSVTNGLDQ
                     LEGNLSAFVKSKSAQVVMLLGNSRIEGGESPNLPFLKQGKWGITVVALGPKTDRRACV
                     DFARKSGGVFIQYEGDPAKVIARLQSR"
BASE COUNT          908 a          612 c          879 g          770 t
ORIGIN      
        1 tcactccagg acacgctagt gctcgcagat ggccctggag taatacagct attccgcaca
       61 ggggagccct tctccgcgga gcatcatgag tgaaacatgc tgaggggagg atagtataag
      121 gaagagtgat atagaaaaat atgagagtta agggtactat tagttcatta aagaaaggat
      181 gcttattatg atggataaga tgaaacaagc caagcagctc tttgatcttc agaaaaaagc
      241 gaaagcaatg caaaaagaac ttcgggatac cgaaattgaa gcagtctcaa atgatggcaa
      301 gattacggct gtgtttaacg gggagcttaa gctcgtagat ttgaagattg ctcgtgatta
      361 catggataat aattcacttg atactttgga aaagtctatt aaaacgaccg ttacggaagc
      421 tctttcgaag gcgcagcaag tcgcagctga gaagactcgc gacattatga aagatatgaa
      481 tctcaatatt ccaggattgt aatcccagtg aaattgattg ttggacttgg aaatccaggt
      541 gagaagtatg cccgtacccg gcataacttg ggttttatgg tgctcgacca tatggcggaa
      601 ttgtttggag cgtcgttttg cgaccaatcc aagtttaagg ccgcaacggc agaaattcat
      661 agtgggggag agaaagtaat tttggttaag ccactcacgt ttatgaacct ttcgggcgat
      721 gcggttatgg cgttggccaa cttttataag ctcgaaacag gtgatatatg ggttattgct
      781 gatgatttag acttgccctt agggaaggtc cgcgtccgaa atgggggaga gtcgggggga
      841 cataacgggc taaaggatat tatggccaaa cttggtacgg gtgacttttg gcgtatccga
      901 gttggaattc ggggggagga agttcgagag ctcaaccacc aaaataatat tacaactcag
      961 gactatgtgc ttgctcgttt tgccgacaac gaaagtaaaa tgttgtactc tacgcttgaa
     1021 tcagtcgcgg tactgctgaa ggagagtatt gaacgtaatc acctagtagc tcatacctac
     1081 caagcaaacg gttttgatga acatattggt acctagcgaa gtcgtgactt gaagattttc
     1141 ctgttttaag ctatacttat caggtgttac aaatgtggga gccgggaggc gtttgaacca
     1201 caaaaggagt tactcatgtc tgagaaccaa aagacaactc gtgagcgtat ctcaaagcag
     1261 acgctcaaag cccgcttagc tgaaaagcga agtgaaaata aaaagcattc gactcgcttt
     1321 ttaaacgaag aagccaaaat tgccgataaa acgaaacagg cgcttccttt ggaagaagct
     1381 cttggattag tgaaacaaac tgcttctact aaatttgata gttcggtgga attacacatt
     1441 catcttaccc ctaagaaggg taaaaaaggt gtcgaagatg aattaatgcg tggcatgatg
     1501 catttgccac aagggcttgg aaagactcgt aaagttgtta tcttgaatga agagattatc
     1561 gagacgttag ccaagaccca aaacgttgat tttgacattg cccttgctac ccccgcatta
     1621 atgccaaaac ttggtaaagt tgctaaactc cttggaactc gcggtaaaat gccaaatcca
     1681 aaagccggta cggttactga taacccagaa caggtgaaag cagaaatcga agcaggtcgt
     1741 gttgagttcc gtcaggacgc cactcgtaac attcatcaga tgattggtaa atcttcttgg
     1801 gatgttgaaa agttagccga caacgcgcga gcagtaatga aatcatttcc caagaaccgc
     1861 gtcatgagcg tagcggtaac cagtaccatg gggccaagcg tctccgtcgt agccgaataa
     1921 gcttacttca agaaagtaga gggcagggga gtgaaaagag cgctgaaagg cgctctttta
     1981 ctatttatat aaatttaaga atagagtggt ttataaaaag aagtaccttt tattttttgt
     2041 ggcatggggg aaaccatgaa gtggtggacg ctgattctca gcatcgcagc ggtatcagca
     2101 atggcggctc aactgagagc gccgtatggg acggagctgg gtcgtattga tggggtcgta
     2161 gctttctcga gtggagacag gttgtggaag tcgcctaaag aaggcaacca gtatcaatgc
     2221 gttgacttgg taaagcggtt tgtccaatcg aagggagtga agtgggtatc cctggacggg
     2281 caagcggcgg caagtttatt tcccatggta gaagcaggtc gtgtccccgg tctgacgagc
     2341 tttgtaaatg gaggcaatga acagccgcag cgaggagatg tggtgtgctt tagtgatggt
     2401 ggtctgggac acatggggat cgtgtacgaa gttcaaaacg acgaggttcg tctgattgaa
     2461 cagaattggc acgcccagcc aaacctctac ttaggactac ctctgaagaa aacagagggc
     2521 atgtatgagc ttgaggggag aacgacaaag ccattgaaag gggcaatggt attctggcgg
     2581 gtgcaagggt gggtacgggt gaagggaccg cctcggcctc tctttccgag ctacacgtat
     2641 gttctcgtcg atacatctgt tgagcgagat tcgcaacttt ttcagcagtc gcagtatctt
     2701 tgtcggcaga tactcaaccg gctgcccgat gaagcgaagt tctcgattat tctggtgcac
     2761 gaaacctcgg agcaaattgc gattgatcca gtgaagaact ggaagtcgga cctcacgtat
     2821 gtgtatcgtc attacgtgaa ggtgaagggg cgccgagtaa gtgttaccaa cgggttggac
     2881 cagcttgaag gaaacttatc ggcatttgtg aagtcgaaaa gcgcccaggt ggtgatgctt
     2941 ctggggaact cgcggatcga aggaggggag agtccaaacc ttccgtttct caagcaaggg
     3001 aagtggggaa ttacagtcgt cgcactcggt ccaaagacag ataggcgggc atgcgtggac
     3061 tttgcccgaa aatctggagg tgtgtttatc cagtatgaag gggatccggc aaaggttatt
     3121 gcccgtctgc aaagccggta agggcaagtg gatcgggctt tcccggggt
//
LOCUS       DASWYB010000039         8227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_694936, whole genome shotgun sequence.
ACCESSION   DASWYB010000039 DASWYB010000000
VERSION     DASWYB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694936"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGE59_02345"
     CDS             complement(<1..434)
                     /locus_tag="VGE59_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074518.1"
                     /translation="MWRFWEWRKKGRPKTDREAVQDVPVHFTLVEAQKVRVVTNSPEW
                     SQGLSDRGFSVSQEDDPSRLLDLVAHFKPEVVVITVRLDHGASKFDLPLRVEQAMETS
                     LLLLAVQNQLYDWTELTHEWAGLTRRQLGESCLVGRAPTDPEV"
     gene            complement(576..1274)
                     /locus_tag="VGE59_02350"
     CDS             complement(576..1274)
                     /locus_tag="VGE59_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074519.1"
                     /translation="MRWLRWLVVCAGLLLGIVTPARADVVWDQSFIEREMARNRFALQ
                     AAQLVVPELKEMLGDPTFPIYVTLTDDVYGSLQRLHKGRPLSEGHQENIANGVHPLAA
                     TTWDRDVNAVRVFILTSQYETAKFPQLCCTLTHEFTHGKSFYDKKKGKIAASKKLSVT
                     VEERRWEKYVYEVTIQGWVVILQDGSLAQLLLQAPGPLDPGSFLRAIDVEVDADRGKL
                     RGYEKLLSEPQMKN"
     gene            complement(1482..2747)
                     /locus_tag="VGE59_02355"
     CDS             complement(1482..2747)
                     /locus_tag="VGE59_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074520.1"
                     /translation="MITQYTDIRCPMYGRIDMRQVHHTLRRREVQRMLRVSQLGAVSY
                     VFPGATHNRLMHMIGVYYITKLRADDLLNRKLITEQEHRDICLTGLVHDIGHEILSHM
                     LELLRGNHDDAGVRLVLGELAEAIRLDGGNPEAIAAHMRRETKLSQLVFGAPCGADTL
                     DYLARDAHQVSGEVLQFDALFCANVRWSREHGLYLLPRGMPQILSVMNRYWHQYCDIY
                     ERISVRLAQRYLQELLRQVIELDPSVCRYLHVSGEAGTMGAVRLWCEDHPETEAARRH
                     ELWLERRYPKKAFVYSPWPQSVPVRDKDSVRKIACDVALLEGSELWSPEALSRKEQEV
                     AELLGLAPEEVSIAPSPPSRRWRALNMPVMSCGEVVSLTSLFPAIQSAGEMYGQMSRN
                     VVASFTEEARARLIQSPEAARKVLQVLER"
     gene            complement(2894..3496)
                     /locus_tag="VGE59_02360"
     CDS             complement(2894..3496)
                     /locus_tag="VGE59_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY1074521.1"
                     /translation="MRKIIVLMMVSLDGVMQAPGGPEEDTSGDFKYGGWTAPYSDESF
                     GSILGAELSMPFDLLLGRKTYEIWASYWPQETGLVADPFNKATKYVVSDKDVDLPWKE
                     SILIQGDVTSKIKALKEENGSPFQVWGSGKLIQTLLKHDLIDELRLRIYPVTIGSGKR
                     LFAEGTIPAAFKLIDSQALPSGVILANYQRAGEFKTGSVE"
     gene            complement(3501..4244)
                     /locus_tag="VGE59_02365"
     CDS             complement(3501..4244)
                     /locus_tag="VGE59_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1074522.1"
                     /translation="MDTLKINSLSLIIHGSSDATKLAVVLPGFLDTKDYPHMKGHVEF
                     LGNNGYLAVSFDPPGTWESDHDISNYTITHYLEAIDEVIAHFGNKPTLVVGHSLGGAM
                     AMLAACRNPHVQGFVAIMSPYAYVRDDNEEKKLVEWQPGEVKVSTRDLPRNPDESREF
                     KLPYSFIEDAIHYNALEGLKTLTKPKLFIIGKDDDVVPGLVTERAYTEAAEPKKLVVL
                     DSDHDYRWHPELIEEVNQLLGAFCETLLS"
     gene            4387..5085
                     /locus_tag="VGE59_02370"
     CDS             4387..5085
                     /locus_tag="VGE59_02370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1074523.1"
                     /translation="MRVLVTGGTRGIGLATVRLFAGLDGCQLVVLANDFTDFPLQGEN
                     VQRDNFDLSQIDAIPKKVHEIGAIDVLVNNAGIYNGRPFSAYTTEDALRIHTVNYLAP
                     MKLATEFVSQFPSSSPSRIINVTSVVAEIGGLDCPYTASKAALQNGTRALARQWGRLE
                     ICVNAVAPGIVEGTDVFNAIPAEVKQRLLAQTMDGKFATPDQVASVIFWLATGAPPSI
                     KGATISINNGSHVS"
     gene            5297..5959
                     /locus_tag="VGE59_02375"
     CDS             5297..5959
                     /locus_tag="VGE59_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074524.1"
                     /translation="MEKKFTLTVGGRNLGEMQAAARKMGWELSPYWYTMQHSWRGFVP
                     TPEPIERTFVELTVGELMGIGQGEFAYPEDILAAIDANRRFRRCPDDAALYARLQPRL
                     TGVKRQQELLLNWLYRVIQRRVGQHWKGLKRIYERLIPERYWITFISNGTVIRGKNGK
                     FYLSFLSLVAGWVDGTDGTLEPNQADYAIHVDADGNRIQNDSVPRQKWPWDLQLVVVL
                     AE"
     gene            complement(6027..7118)
                     /locus_tag="VGE59_02380"
     CDS             complement(6027..7118)
                     /locus_tag="VGE59_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY1074525.1"
                     /translation="MNQRILIIGAGLGGLALYKALDKNKFTVHLADKRRELGSLGYGI
                     LFYPIGGYALRRLGFSSDFVQNLGKSYSQYYFRNEDATLRDIIDYSSVSQEFGDYKGV
                     SRGDLYKPLFADINSDDLLLGNRVVGMSESSNEVQVTFEDGTIRTYDFVIGADGAHST
                     VRQSILPDSQLEKLGATFMWTWVLKSELEAIPPRPTLYFGEKANLGVLDVNDPERVVA
                     FCWFTEDALGREASHEEWNDYIKEHFSSFGGIVPSVLRSLYKQHDRFVHRDYQLKLPT
                     WHKGRVGLIGDAAHALSALTGLGSALALEDGAFLAELFNKTDDLGSAFQHFENIQRPR
                     TINVKLEDFATTEGSLSIMREIFSSSPLR"
     gene            complement(7222..7959)
                     /locus_tag="VGE59_02385"
     CDS             complement(7222..7959)
                     /locus_tag="VGE59_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY1074526.1"
                     /translation="MLEHFSIFVIGLLAGFFDSVVGAGGLISTPALLLLGLSPQTAIA
                     TDRFGSLGQSIAAFFSFNKAKKINWNYVWIFSLLSLAGSLIGARILLHVDTDILQRIV
                     AILILALLPLIIFGKKKETTMPKPTARRVALGLFSYFVIMTFGGFFGQGTGPLMFYAL
                     SYFLGFTLVEILATGIIPWLVLSASSTFVFAINGIIDYQKGLVLFVGMAVGGYLGAHL
                     AVTKGDGWLKRLFIIFVVVASIKLLFF"
     gene            complement(7969..>8227)
                     /locus_tag="VGE59_02390"
     CDS             complement(7969..>8227)
                     /locus_tag="VGE59_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074527.1"
                     /translation="LDSFISQLNMDRDILFVWLRTNEKVRLSRKEKRNRDLADTKDHF
                     EFVDRRYPDIDSINVKNGTSVFIDTSAKTVQDVVSEIKTLI"
BASE COUNT         2252 a         2207 c         1841 g         1927 t
ORIGIN      
        1 acctctggat ccgtcggcgc gcgtcccaca agacacgact cgccgagttg ccgccgggta
       61 agccccgccc actcatgagt cagttccgtc cagtcataca actggttttg aactgccagc
      121 aagagcaacg acgtctccat cgcttgctca acccgtagcg gcagatcaaa cttgctcgca
      181 ccatgatcca gccgaaccgt aatgacgacc acttctggtt tgaaatgggc cacaagatcg
      241 agcaaacggc tcggatcatc ttcctgagac acactaaacc ctcggtcaga aagcccctga
      301 gaccactccg gtgaatttgt gacaactcga accttctggg cttctaccaa ggtaaagtgg
      361 actggcacat cctgaacagc ttctctatcg gttttcggcc gccccttctt tctccactcc
      421 cagaaacgcc acatgatcta tctccgcggc ccgatggcca acataaaata acctctgccg
      481 ttaacgcctt agactatact ataaaaatca tattttgtct atataaaata agcgggccct
      541 cccggaggag gacccgtctc gaaccagctc gcaggctagt tcttcatctg tggctcgctg
      601 aggagcttct catagccccg taacttcccc cgatccgcgt ccacctccac gtcgatggca
      661 cggaggaacg aaccggggtc gagcggaccg ggagcttgca gcagaagctg agcgagggaa
      721 ccgtcttgga ggatgacgac ccacccctga atcgttacct cgtagacgta cttctcccat
      781 cgccgctctt ctacggtcac cgacagtttt ttcgaggcag cgattttacc ttttttcttg
      841 tcgtaaaacg acttcccatg ggtgaactca tgggttaacg tgcagcagag ctgcgggaac
      901 ttcgccgtct cgtactgaga cgtgagtatg aagacccgca cggcgttcac gtcccgatcc
      961 caggtcgttg ccgccaaggg atgtactcca ttggcgatgt tctcctggtg cccttccgat
     1021 agcgggcggc ccttgtgcag ccgctgcaac gacccgtaca cgtcgtccgt aagggtgacg
     1081 tagataggga aggtgggatc tccgagcatc tccttcagct cgggcaccac cagttgagcg
     1141 gcctggaggg cgaacctatt gcgcgccatc tcccgctcaa tgaacgattg atcccagaca
     1201 acgtccgccc gagcgggcgt gacgataccg agcaaaagcc cggcacacac aaccaaccac
     1261 ctcaaccaac gcatggcgtc ctctccttta ccgtacctca aatgcgtcta gatggattat
     1321 actttaaata gggtgaaggg tcaataattc ataaaggtac gatatgcaaa acagaaataa
     1381 tgcgaaagaa gcctaatatc actattaact ggtaaacttt cataaaaaac acaaagcctc
     1441 cggacgaacc gaaggctcgt gcacggaggc cgatatttgg tttaacgctc gagaacttgc
     1501 aaaaccttac gagcggcttc tgggctttga atgagtcgcg cccgagcttc ttctgtaaaa
     1561 ctcgccacga catttcggct catctgtccg tacatctctc ccgcactctg gatcgcgggg
     1621 aagagcgacg ttaacgaaac aacttcgcca caactcatca ccggcatgtt aagagcccgc
     1681 cagcggcgag aagggggaga gggggctatc gaaacctcct caggagcaag cccgagcaac
     1741 tcggccactt cctgttcttt ccgcgacagt gcttcaggcg accacaattc actcccttcc
     1801 agcagcgcta catcgcacgc tatcttacga accgagtctt tatcccgcac tggcactgat
     1861 tggggccagg gagagtaaac aaacgccttc tttgggtacc tccgttccag ccataactcg
     1921 tgccgacgtg ctgcctctgt ctctgggtga tcttcacacc acaggcgaac cgcccccatt
     1981 gtaccggcct ctccactcac gtgaaggtag cgacacacac ttggatcgag ctcaatcact
     2041 tggcgcagca actcttgcaa gtaacgctgc gccagccgaa cactaatccg ctcatagatg
     2101 tcgcaatact ggtgccagta acgattcatc acagacagaa tctgcggcat accgcgtggc
     2161 agcaggtaca gaccgtgctc cctgctccag cgcacattgg cgcagaacag ggcatcgaac
     2221 tgcagcactt cgcccgaaac ttggtgcgca tcgcgagcca agtaatcaag cgtatcagcg
     2281 ccacaaggag caccaaacac aagctgagaa agcttggttt cacgtcgcat gtgagccgca
     2341 attgcctcag gatttccacc atcaagccga atcgcttcgg cgagctcacc aagcacgagc
     2401 cgcacaccag cgtcatcatg gttgccacgc aacagctcga gcatatggct gagaatctcg
     2461 tgacctatgt cgtgaacgag tcccgtgagg caaatgtctc gatgctcttg ctctgtgatc
     2521 aatttccgat tgagcagatc gtctgcgcga agtttggtga tgtaatacac accaatcatg
     2581 tgcataagac gattgtgcgt cgcaccggga aacacgtatg aaacggcgcc gagttggctg
     2641 acacgcaaca tgcgctgcac ctcacgccgt cgaagcgtat ggtgcacctg ccgcatatca
     2701 atacggccat acattggaca acgaatgtcc gtgtactggg taatcattcc gttctccaaa
     2761 tgcaccagaa gtgcaccaag ggggccaaat tgcaccacct gatttatacc atatataaaa
     2821 tgaagaatac aaaaatataa gcgtagtaca tatgtactat atgcccatta aaaaaactta
     2881 tgcgtacgta cgcttattca accgaacccg ttttgaattc gccggctctt tgatagttcg
     2941 ccagaataac accgctcggt aacgcttgag agtcgattaa cttaaacgcc gcggggattg
     3001 ttccttctgc aaataggcgt tttccactcc caatcgtaac aggataaatt cggagccgta
     3061 attcatcaat caaatcatgt ttgagaagtg tttgtataag tttgccgcta ccccaaactt
     3121 ggaatgggga gccattctct tcttttaacg ctttaatttt actcgtcaca tcaccctgaa
     3181 taagaattga ttccttccaa ggaagatcga catctttatc cgacacaaca tattttgtag
     3241 ctttgttaaa cggatcggca accaagcctg tctcctgcgg ccaatacgac gcccatatct
     3301 cgtatgtctt cctgccgagc aataagtcaa agggcatact caattctgcg ccaaggatac
     3361 ttccaaacga ttcatcggaa tacggggcag tccagccgcc atacttaaaa tctccggacg
     3421 tatcttcttc cggaccaccg ggcgcctgca taacgccatc aagactcacc atcattaaaa
     3481 cgattatttt tctcatattt ttatgataac agagtctcac aaaaagcgcc taacagctgg
     3541 ttcacttcct caatgagctc aggatgccag cggtagtcat gatcggaatc taaaacaacc
     3601 agcttctttg gctcagcagc ttctgtatac gcccgctcag ttacgagccc aggcacaacg
     3661 tcatcatctt tcccaatgat aaacagcttc ggcttcgtta aagttttgag cccctctaaa
     3721 gcgttatagt gtatagcatc ttcaataaat gagtagggga gcttaaactc gcgggactca
     3781 tctggattac ggggcaaatc acgcgttgag acttttactt cccctggctg ccactctaca
     3841 agcttctttt cctcattatc atcgcgaaca tacgcgtatg gcgacataat cgcgacaaaa
     3901 ccttgcacgt gcggattccg acatgccgcc aacatcgcca tcgcaccacc caggctatgt
     3961 ccaaccacaa gagtgggctt atttccaaag tgagcgatca cctcatcaat cgcctccaag
     4021 tagtgagtaa tggtgtagtt gctgatgtcg tggtcacttt cccacgttcc aggtggatca
     4081 aacgacacag ccaaataacc gttgttaccc aaaaactcca cgtgcccctt catatgaggg
     4141 tagtctttcg tatccaaaaa acccggaagc accaccgcta actttgttgc gtcgctacta
     4201 ccatggataa taagagacag tgaatttatt ttgagagtat ccatacaccg agtataccaa
     4261 agataaataa taacgatact tttttggatt attctaacct cagacgaaaa ggcactaaaa
     4321 cttctaacca taataaggta taatctactc gtgctgtttt tacctgatcg atagacggag
     4381 gacacgatga gggttctagt tacgggagga acaaggggca tcggcttggc aaccgtcagg
     4441 ctctttgccg ggctagacgg atgccagctt gttgttctgg ccaacgactt cactgatttc
     4501 ccgcttcagg gagaaaacgt tcagcgagat aacttcgatc tcagtcagat cgatgccatc
     4561 ccgaagaaag tacacgagat cggagcgatc gatgtactcg tcaacaacgc cggcatctat
     4621 aacggccggc cgttcagcgc ttacacgacc gaggatgcgc tacgaataca cacagtcaat
     4681 tacctcgccc ccatgaaatt ggctacggag ttcgtaagcc aattcccgag ctcatcccct
     4741 tcccggatta tcaatgtcac ttccgtagtg gcagagatag gcggcctgga ttgcccttac
     4801 accgcgagca aagcggcgct ccagaatggc acacgcgcac tcgcacgaca atggggcagg
     4861 ctcgaaatat gcgtcaacgc ggttgcaccc gggatcgtgg aaggaacgga cgtattcaac
     4921 gccattcccg ccgaggtcaa acagcgcctg ctggcgcaaa cgatggatgg caagttcgca
     4981 acccctgacc aagtagcctc cgtgatcttc tggttggcca ctggcgcacc cccgagcatc
     5041 aagggggcaa ccatctccat taataacgga tcacatgtga gctagactca ccattttcaa
     5101 ccggcagcgc cggaaataaa tgcaggcggc ctcttgtata ggcctcctgc attttgaatt
     5161 ataaaaacat tcataataat cttgacaaat agacctgttt agattatact attttaaatt
     5221 cctgtcatat agtcaggtga acggggctct ctacccccac tagagaccgc atcttgggag
     5281 acaaggggga aacaagatgg aaaagaaatt caccttaacc gtaggcggtc gcaatttggg
     5341 agagatgcag gccgccgcgc gaaaaatggg atgggagctg tctccctact ggtacacgat
     5401 gcaacattcc tggagggggt tcgtccccac cccggaaccg attgagcgaa ccttcgtcga
     5461 actcacggta ggcgaactca tggggatcgg ccaaggagaa ttcgcctacc cggaggacat
     5521 cctcgcagca atcgacgcta acaggcgatt ccgacgctgc ccggacgacg ctgccttgta
     5581 cgcaaggctg cagccgcgcc tcaccggcgt taagcgacag caggagctct tgctcaactg
     5641 gctctatcgg gtcatacaac ggcgagtagg tcaacactgg aaagggctga agcggatcta
     5701 cgaacggctg atacccgagc ggtattggat cacctttatc tccaacggca cggtgatccg
     5761 tgggaagaac ggaaaattct acctgtcctt cctctcgctc gttgcgggat gggtggatgg
     5821 cactgacggt accctggaac caaaccaggc cgactacgcc attcatgtcg acgcggatgg
     5881 taaccgcatc cagaacgata gcgttccccg ccagaaatgg ccgtgggatc tccagctagt
     5941 cgtagtgctg gccgagtagc cagcaatgtc tcagcgggtc cgtgcgtagt aatacgcacg
     6001 gacccgtttt gattcttgtg ggatttctaa cgtaatggac tcgagctgaa aatttctcgc
     6061 atgatgctta acgagccttc ggttgtagca aaatcttcta atttaacgtt aatagtccgt
     6121 ggacgctgaa tattctcaaa atgttggaat gcgcttccta aatcatctgt tttattaaag
     6181 agttctgcca agaaagcccc gtcttctaaa gccaatgcac tcccaagtcc cgtaagagca
     6241 gagagcgcat gagcagcatc accaatcaga ccaactcgac ctttgtgcca cgttggtaat
     6301 tttagttgat aatcgcggtg aacgaagcga tcgtgctgtt tatagagaga ccgtagcacc
     6361 gagggaacta tcccaccaaa ggaagaaaaa tgctccttga tataatcatt ccattcttcg
     6421 tgacttgctt ctctgccgag cgcatcttca gtaaaccagc agaaggcaac aactcgttca
     6481 ggatcattta catccagaac ccccaagttg gccttttctc caaaatacaa ggtaggtcgt
     6541 ggcggtatgg cttctaattc actttttaat acccatgtcc acataaaggt agcgcccagt
     6601 ttttctaact ggctatcagg tagaatgctc tgccgaaccg tagaatgagc gccatcggct
     6661 ccgattacaa aatcgtaggt acgaatcgtt ccatcttcaa aagttacctg aacttcgttg
     6721 ctgctctcgc tcattcccac aacacgattg cccaagagaa gatcgtcaga attaatatca
     6781 gcaaagagtg gtttatagag gtcaccacga ctcacaccct tataatcgcc aaattcttgc
     6841 gatacagaac tataatctat aatatctcgc aacgttgcat cctcattacg aaaatagtac
     6901 tgagagtagg acttcccaag gttttgaaca aaatctgagc taaagccaag acgacgaagg
     6961 gcatacccac caattggata aaatagtatg ccatagccaa gactgcccag ctcacgccgc
     7021 ttatcagcaa gatgaaccgt aaatttattt ttgtcgagag ctttgtagag agcaagccca
     7081 ccgagaccag cgccaataat gaggatacgt tgattcattg agattaattt ccttatatac
     7141 cttattatag tcagatttta aaaatttgcc tagagatatt taggtttttt cagcttgaag
     7201 ctcttgagat gaaaaccaag cttagaaaaa aagtagttta atacttgcaa ccactacaaa
     7261 tataataaat aaccgtttta accagccatc tcccttagtt actgctagat gggcgccaag
     7321 gtatccaccg acagccatac ccacaaaaag tacaagccct ttttggtaat caatgatccc
     7381 atttatagca aagacgaaag tacttgaagc agacaagaca agccagggga ttatccctgt
     7441 ggccaaaatt tctaccagtg tgaacccaag gaagtaacta agcgcataga acatgagcgg
     7501 tccagtacct tggccaaaga atcccccgaa ggtcataatc acaaagtaag agaaaagacc
     7561 cagcgcaacc cttcgagcag ttggcttggg cattgtcgtc tctttctttt tgccgaaaat
     7621 aatgagaggc aaaagtgcaa gaattaagat tgcaacaatt ctttgtaata tatccgtatc
     7681 aacatggaga agaattcgcg cgccaattaa agacccagcc aaactaagta acgagaaaat
     7741 ccacacatag ttccaattaa tttttttggc tttattgaaa ctaaaaaagg cagcaatact
     7801 ttgccctagg gatccaaacc tatcagtagc aattgcagtt tgtggcgaca aaccaagtaa
     7861 tagtagtgcg ggtgtagaga tcaagccacc ggccccaaca actgaatcaa aaaagccagc
     7921 gagaagccca ataacgaaaa tagagaagtg ttccaacatg gagataggtt aaatgagcgt
     7981 tttaatttca ctcactacgt cttgaacagt ttttgcagat gtatctataa aaacagaggt
     8041 gccatttttt acattaatag aatcaatgtc cgggtagcgt ctgtccacaa attcgaaatg
     8101 atcttttgta tctgccaagt cccgatttct cttttctttt cgagatagtc gcactttctc
     8161 attggtcctt aaccaaacaa ataatatatc tctatccata tttaattgtg agataaaact
     8221 atctaat
//
LOCUS       DASWYB010000040         6717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_191342, whole genome shotgun sequence.
ACCESSION   DASWYB010000040 DASWYB010000000
VERSION     DASWYB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191342"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VGE59_02395"
     CDS             complement(<1..740)
                     /locus_tag="VGE59_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074528.1"
                     /translation="MPADQLERPSNMPEGEGNDYDLKNLRANRNQSSETDPEEARLRN
                     LRSPGIPTPQNYNLQDLEPYRRLDSQLNDGENVSDPGFGQDLTQADEAPVRSQPFQDI
                     RSTQGDTITDRALERAKERYANRASRGVPGADQSPSLQNGRTGEKLSSNSFKNITAKK
                     GTPWNPPAASQVTNKGLEKAGQQVGKATAKAAASAGAKVAGKVGTKVAVGAAAGAATG
                     GAATAIQLAYEAGSALFQKFNTAQNRKR"
     gene            complement(822..2702)
                     /locus_tag="VGE59_02400"
     CDS             complement(822..2702)
                     /locus_tag="VGE59_02400"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEY1074529.1"
                     /translation="MSKKKTKSQSPKRSLPKFLSLRKVPRPVVLSVAGVLGIVALCAL
                     LLAGFTLRYQNRIYPNTYIGSIDLGGKNTTQAKQILDEKIAQFPKNISILIDTKTHTI
                     SASEIKLEYQTEKTIEDLLSIGRVGSFSQNIRELLASTFSKHQRQMAFTLDEAKTDQF
                     LTKIANQTGDLPQDAKVILGENDTVTIQSAKAGNGVLPAQLHANLRTALGLFQTSFEA
                     TISPLAPTINDAAAADALTQTQAILAKAPLTFTVGEAKTIADVDRVFSWIAYDVKKSP
                     TQSSGWIPFARATEADTTLVVRLDKDKIKSFVQDFAETINVEPENARLLAVDNKITVV
                     KEHVDGKQVQLTESIDLITSQLTNDSPGALTIALPSATTVAEVRSNNIEELGIRELIG
                     RAETTYKGSPENRQHNIATGARFLNGLLIPPGQEFSTVKTLGKVDGSTGYLPELVIKE
                     NKTTPEFGGGLCQVSTTLFRATLNAGLKITERSNHSYRVSYYEPPLGLDATIYLPKPD
                     FKFLNDTPGYILVQNKIEGTKITFELYGTKDGRTSSLSDTQITSTTPAPPAEYGNTDT
                     LAKGVVKQIEKAHDGATTVVNYVVTRNGQEINKQVFRSRYKAWPAKYLVGTREDTPPT
                     TQ"
     gene            complement(2683..2916)
                     /locus_tag="VGE59_02405"
     CDS             complement(2683..2916)
                     /locus_tag="VGE59_02405"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEY1074530.1"
                     /translation="MHSLTATIDRFERGRAVLVFDDGQELILPKRKLPGKIQEGSVLF
                     CEIYPAEDAEIRKENIARYLLKEILHTNEQEKD"
     gene            complement(3012..3536)
                     /locus_tag="VGE59_02410"
     CDS             complement(3012..3536)
                     /locus_tag="VGE59_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY1074531.1"
                     /translation="MNLSHLPIGKKAPDVVNIVVEIPAGSKNKYEYDEEMHTIKLDRV
                     GYTAMVYPYDYGFIPETRSEDGDHLDAFVLLDHSVFHGCVVAGRPIGMMKMIDSGEMD
                     EKIICVPANDVRYDHIESLEQLSPHVTKEIQHFFEHYKDLQGKKVEISGWFNAHEAKQ
                     TILEAQATEAKRNA"
     gene            complement(3624..4745)
                     /locus_tag="VGE59_02415"
     CDS             complement(3624..4745)
                     /locus_tag="VGE59_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY1074532.1"
                     /translation="MARICIDARFYSTGTGVARYIKALLHQLQRLDTENEYTVILKPE
                     SMVEWNSQPHPKNWRVKTLDIVHYSVYEQTKLLSYLNKEKFDLVYFTMFNHPIFYRGK
                     RVIMIFDLIMHFYPPRPLWHPRTWAYRYMMWHAAHWAHRVLNDSQSAKDDVIKHLHVP
                     AKKCTVTLLAVEDKFQPEHNVKRFDELKSKFGLTKPFLFYVNAWRPHKGLPELVEAFL
                     EVKKNHDIQLLISGKPNPSFPEVIESVTHGQAQTSDIITPGFISDEDVISLYSMAEAY
                     INPSHYEGFGLGPLEAMASGCPVISAKNSSIPEAVGDAGLYYKTGDSHDLAKKIDTLL
                     ADKKLKEELRDKGLAHVKTFSFEKMAQQTLMVFDEVLKS"
     gene            complement(4747..5835)
                     /locus_tag="VGE59_02420"
     CDS             complement(4747..5835)
                     /locus_tag="VGE59_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HEY1074533.1"
                     /translation="MKNLSLSVFFPAYNEETNIESSVSRAREVLQQITSLYEIIIIND
                     GSSDATGAIADRLAKKYPNVRVVHHPGNRGYGEAVKSGFAAARYDYVFFTDADLQFDL
                     TEIKKLIQYIPEYQVVIGYRAKRRDPFLRTLNAKGWNLLNRLTFGLKVKDIDCAFKLF
                     DRTVIKNIHLQSGGAMVSAELLIRLQRQGTIFKEVPVTHIPRAAGSPTGAKPSVILRA
                     FKEMIIAYRGDLGNVTQRQAIKFMTVGFANTCLDLLLYFIFSRYISFFVNHRVIAKMA
                     TYGIASLSSFIFNRRWTFRRTSTIHMSEVIRFYVTVGFALIINAGTMYILIGLKVNDI
                     IAAPIATLVSFVWNFLVSKFWVFTHKKG"
     gene            5960..6643
                     /locus_tag="VGE59_02425"
     CDS             5960..6643
                     /locus_tag="VGE59_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1074534.1"
                     /translation="MHLVIVIPAYNESRVIGDVIASLPKTLPGIRRITPLVIDDNSSD
                     ETRLCARKAGALCISHGANLGAGGATITGFEAAMLLKADIVVTMDGDGQHDPEDLPNL
                     INPIIQKKADVVFGSRLLNNMGNMPLYKKFGNNLMNIITYIFYRIWISDSQSGYKAFS
                     KYALRRIRLSTSGYEFCSEICGQIKQKKLHFIEVPIKTIYTDYSRAKGQLALNAVNIV
                     VGLLLSKIR"
BASE COUNT         1719 a         1347 c         1619 g         2032 t
ORIGIN      
        1 atgcgctttc gattttgagc agtattaaat ttctgaaaaa gagccgaacc tgcttcgtac
       61 gcaagctgaa tagccgttgc tgccccgccg gtagccgctc cagctgcggc gccaaccgca
      121 actttagttc ctactttacc cgcgactttc gcgcctgcac tggcagccgc ttttgctgtc
      181 gcctttccaa cttgttgtcc tgctttctcg aggcctttat ttgtgacttg ggaagcagca
      241 ggaggattcc agggtgtccc tttttttgcg gtgatatttt taaacgagtt ggaggagagt
      301 ttctcaccgg ttcttccgtt ttgtagcgaa ggactttgat ctgctcctgg cacaccgcgg
      361 cttgctcgat tcgcgtagcg ttcttttgcc cgctccagtg cgcggtcggt gatagtgtct
      421 ccctgtgtcg atcgaatatc ctggaacggt tgggaacgaa caggcgcttc gtcggcttgt
      481 gtaaggtctt gcccaaatcc tggatctgaa acgttttctc catcgttcag ttgggaatct
      541 agcctgcggt aaggttctaa atcttgaaga ttgtaatttt ggggagtagg gatgccggga
      601 gaacgtaaat tacggaggcg ggcctcttca gggtctgtct cggaagattg attgcgattt
      661 gcccgtaaat ttttgaggtc gtaatcgttt ccctcgccct cgggcatgtt tgatggtcgt
      721 tctagctggt cagctggcac tcggcccctc ctaggctgta ctactatagt agcataagtc
      781 agtcggggag gggcaccttt ttacggatcc aatctaacac attattgtgt ggttggaggt
      841 gtgtcttcgc gagtacctac aagatactta gcgggccagg ctttataacg tgaccgaaag
      901 acttgtttat taatctcttg gccgttacga gttacgacgt aattcacaac agtagtcgcg
      961 ccatcatgag ctttctcaat ttgcttcacg actcctttag ccagagtatc agtgttacca
     1021 tattctgccg gaggggcagg agtcgtgctc gtgatctgcg tatcactcaa ggaagatgtt
     1081 cggccgtctt ttgtgccgta gagttcaaat gttattttgg tgccttcgat tttattctgg
     1141 acaagaatgt agccaggggt gtcgtttaga aatttaaagt ctggcttggg cagataaatg
     1201 gtggcgtcta gaccgagagg gggctcgtaa tacgaaacgc gataggagtg gttactccgt
     1261 tctgtgattt ttagacctgc gttaagggtt gcccggaaga gggttgttga aacttggcat
     1321 aatccgccgc cgaattcagg agttgtttta ttttctttga tgacaagttc tggcaaatat
     1381 cctgtggagc catcgacttt tccaagagtt ttaacggtcg aaaactcttg tcctggcgga
     1441 atcaggaggc catttaagaa tctggcacct gtggcgatat tgtgttgacg gttttcagga
     1501 gaacctttgt aggtggtttc tgctcgaccg atcagttctc gaatgccgag ttcttcaatg
     1561 ttgttactgc gaacttcagc gacagtggtt gctgaaggaa gagcaatggt aagtgcaccg
     1621 ggcgagtcgt tggtcagttg tgaggtaata agatcaatgg attctgtgag ttgaacttgt
     1681 tttccatcta catgctcctt tacgaccgta attttattgt cgacggcaag gaggcgagcg
     1741 ttttcgggtt caacattaat agtttctgcg aaatcttgaa caaaactctt tattttgtct
     1801 ttatctaagc gaacgacgag ggttgtgtct gcctctgtcg ctcgggcgaa cgggatccat
     1861 ccgcttgact gagttggtga ttttttcaca tcatacgcaa tccaagagaa gactcgatca
     1921 acatcagcaa ttgtttttgc ttcgccgacc gtgaacgtca ggggggcttt cgcaaggatt
     1981 gcctgggttt gggtgagagc atcggcagca gccgcgtcgt tgattgtagg agcgagggga
     2041 gagatggtgg cttcaaaaga ggtttgaaaa agacctaacg cagtacgaag gttcgcatgg
     2101 agttgcgcag ggagaacacc gttaccagct ttggcacttt gaattgtaac ggtatcgttc
     2161 tctcctaata taactttagc atcttgaggt aggtctcccg tttgattggc gattttcgtg
     2221 agaaattgat cggttttcgc ttcgtcgagc gtaaaagcca tttgtcgttg atgcttcgaa
     2281 aaggtagaag cgaggagttc tcgtatgttc tgagaaaatg agcctactcg gccaatactg
     2341 agaagatcct caatggtttt ctcggtctga tattctaatt ttatttccga cgcagaaata
     2401 gtgtgtgttt tggtgtcgat aagtatcgag atatttttgg gaaattgagc gatcttctcg
     2461 tctaggatct gctttgcttg tgtcgtattt ttaccaccaa gatcgattga tccaatatac
     2521 gtgttggggt aaatgcgatt ttgataacgg agggtaaatc cggcgaggag aagtgcacag
     2581 agagcaacta ttccaagaac ccctgccaca gacaaaacga ccggacgagg gacttttcgt
     2641 aaggacagga attttggaag cgaacgtttg ggagattgtg atttagtctt tttcttgctc
     2701 attggtgtgt aaaatttctt ttaaaaggta gcgggcgata ttttctttac gaatttctgc
     2761 gtcttcggcc ggatatattt cacaaaaaag aactgagcct tcttgaatct taccaggcag
     2821 tttgcgtttc ggtaagatta attcttgtcc gtcgtcaaat actaaaaccg cccgaccccg
     2881 ttcaaaccga tcgatagtgg cagtgagaga atgcataggc atattctacc tgatagcctg
     2941 taaaaggaga aggggcgagt agaaactacc gtgaaggcta agtaaaagcc ccttaattca
     3001 ggggctttta cttaggcgtt tcgctttgcc tcggttgctt gggcttcgag gatggtttgt
     3061 ttcgcttcat gagcgttaaa ccaaccagat atctcgactt tcttaccttg caggtcttta
     3121 tagtgttcaa aaaagtgttg aatttctttt gtaacatgag gactgagttg ctcgaggctc
     3181 tcaatgtgat catagcggac gtcattggcg gggacacaga taattttttc atccatttca
     3241 ccactatcga tcattttcat catgccgatt ggccgtccag caactacaca gccatgaaac
     3301 acgctgtgat ctaaaagaac aaacgcatcg aggtggtcac cgtcttcact gcgtgtttcg
     3361 gggataaatc cataatcgta cggatacacc atcgctgtgt agccgacacg gtccaattta
     3421 atggtatgca tctcttcatc gtattcatat ttatttttgc ttcccgcagg aatttctaca
     3481 acaatattca cgacgtcagg agcttttttg ccgatcggca agtgagataa attcatgcgt
     3541 tcatcctttc tagttgagca ctaaaaactt agctagtata aggttaccac aggttaaaat
     3601 agtctgacaa gggatatgtg ttgttaggat ttaagcactt cgtcaaagac cataagagtt
     3661 tgttgtgcca ttttctcaaa ggaaaatgtt tttacgtgag ccaggccttt gtcgcgcaat
     3721 tcttctttga gcttcttgtc tgcgagcagg gtatcgattt tctttgctaa atcgtggctg
     3781 tcgccggttt tatagtatag ccccgcgtcg ccaactgctt ccggtatgct ggaattttta
     3841 gcactgataa cagggcagcc gcttgccata gcttccaatg gtcctaatcc aaacccttca
     3901 taatgagaag ggttaatgta tgcttcggcc atactataga gagaaatgac gtcctcgtca
     3961 gatatgaatc ccggcgtgat gatatcggac gtttgagctt ggccgtgtgt gactgactca
     4021 ataacttctg gaaacgaagg gtttggtttt cccgatatga gaagctggat gtcgtggttt
     4081 tttttcactt ctagaaaggc ttcaacaagc tcgggtaggc ctttgtgtgg tcgccaagcg
     4141 tttacataaa ataagaatgg ttttgtaaga ccaaatttgc ttttgagttc gtcaaatcgc
     4201 ttcacattat gttcgggttg gaatttatct tctactgcca aaagtgtgac agtgcatttt
     4261 ttagccggaa catgaaggtg tttaataaca tcatccttgg cagactgact gtcgttcaat
     4321 acccgatggg cccagtgggc cgcgtgccac atcatatagc ggtaggccca agtacgcgga
     4381 tgccagagtg gacgcggtgg ataaaagtgc attatcaagt cgaaaatcat aatgacccgt
     4441 ttgccacgat aaaaaatcgg atgattaaac attgtgaaat aaacgagatc gaacttttct
     4501 ttattaagat aagacaagag tttggtttgt tcgtagacac tatagtggac aatatcgagc
     4561 gtctttactc gccaattttt aggatgtggc tgactgttcc actcaaccat ggattcaggt
     4621 tttagaataa cggtgtattc gttttcagta tcgagccttt gaagttgatg aagaagggct
     4681 ttaatatagc gagcgactcc agtaccggta ctataaaatc gagcatcaat acaaatacgg
     4741 gccatattat cctttcttgt gggtaaacac ccaaaacttt gaaacaagga agttccacac
     4801 aaagctcacc agtgtcgcaa ttggcgccgc aataatgtca tttaccttga ggccaatcag
     4861 aatatacata gttcccgcgt taataataag tgcgaatcct accgtgacgt aaaatcgaat
     4921 aacctcactc atatggattg tgctggtccg tcgaaacgtc caacgccggt taaatataaa
     4981 actagaaaga gaggcaatgc catacgtggc catcttggca attacccgat ggtttacaaa
     5041 gaacgaaata taacgactaa agatgaagta gagtaataaa tcaagacaag tattggcaaa
     5101 gccaacagtc ataaatttaa tagcttgacg ttgtgtgacg ttgcccaggt ctccccgata
     5161 ggcgataatc atttctttaa aagcgcgtag aataactgaa ggctttgctc cggtaggtga
     5221 gcctgcagct cgggggatgt gggtcactgg tacttcttta aatatcgttc cctgacgctg
     5281 cagtcgaatc agaagttcag cacttaccat agctccacca gattgaaggt gaatattttt
     5341 aatgaccgta cggtcgaaga gtttgaaagc gcaatcaata tcttttactt tgaggccaaa
     5401 agtgagtcgg ttgagtaaat tccagccttt tgcatttaac gttcttaaaa acgggtcgcg
     5461 acgttttgct ctgtaaccaa tgacgacttg atactccggg atatattgaa tgagcttctt
     5521 tatttcagtc aaatcaaatt gaagatctgc atcggtgaaa aatacgtagt catatcttgc
     5581 cgctgcgaac ccacttttaa ctgcttctcc ataccctcgg ttcccagggt gatggacgac
     5641 acgaacgttg gggtatttct ttgctaagcg gtcagctatg gcaccggttg cgtcactgct
     5701 tccatcatta ataatgataa tctcgtagag ggaggtaatc tgttgtagta cctctcttgc
     5761 gcgagatacg gatgattcaa tatttgtttc ttcattataa gcggggaaga aaactgaaag
     5821 ggataaattc ttcatgagta tggagcgagt atagcaaaaa taataattcc tcgacaattt
     5881 ttatggcctg tgatatgtag atatcgagtt ctctttagta ttcctgtata ataagagtca
     5941 caaagagaga atcgaaaata tgcatttagt tattgttatt ccggcatata atgaatcacg
     6001 agttattggc gatgttatcg caagccttcc gaagacattg ccgggtattc gtcgtattac
     6061 ccctctcgtt attgatgata attcttctga cgaaacaagg ttatgcgctc gcaaggcggg
     6121 agcattatgt attagccatg gggcaaattt aggggctggt ggtgcgacta ttacaggttt
     6181 tgaggccgct atgcttctta aggctgatat tgtagtaact atggatggag atggacaaca
     6241 tgatccagag gatctaccaa atttgattaa tccaattatt caaaaaaagg ctgacgtggt
     6301 ttttggatcg agattactta ataatatggg taatatgccg ctttacaaga aatttggtaa
     6361 taatcttatg aatattatta cttacatatt ttatcgaatt tggattagtg actctcaaag
     6421 tgggtataaa gcattttcaa agtatgcact tcgtcgtatt cgactatcta cgagtggcta
     6481 tgaattttgc tccgaaattt gtgggcaaat taagcaaaaa aaacttcact tcattgaagt
     6541 tcccattaaa actatctata cggattattc tcgtgccaaa gggcagctgg cattaaatgc
     6601 cgttaatatc gtggttggtt tgcttctgag taagattagg taacgtcgta tgattattat
     6661 tgttaaactc attgcgatcc ttgtggctct cgtctcactt attaagagtt atgttga
//
LOCUS       DASWYB010000041         5069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_695843, whole genome shotgun sequence.
ACCESSION   DASWYB010000041 DASWYB010000000
VERSION     DASWYB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695843"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VGE59_02430"
     CDS             complement(<1..722)
                     /locus_tag="VGE59_02430"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY1074535.1"
                     /translation="MTRTSDLSLFHKILVLTEDKGEIVLDDLKQFGSTRQTLGALGRL
                     EGLKLIERTKEEKRNVFTLTEKGDLVLADILGHLPDSEKNWDGKWRIILFDIPESKRT
                     VRQMFRIKLLDFGARMLQSSVWITPNKSVADKFYDLIMQTEFAHTVQFFEAKHFGNNA
                     IDVLSLWQLDKLDKEYRELFKELDTQLKNLKKQKDISFTAKCMIVSLALVAKKDPHLP
                     VELMPKKWAGLEAEAWYKKIRP"
     gene            complement(782..1741)
                     /locus_tag="VGE59_02435"
     CDS             complement(782..1741)
                     /locus_tag="VGE59_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY1074536.1"
                     /translation="MISRILTHRPTVIILLVLAILGLGNTILQHKTASALNNDETLIT
                     IDKELAHSVDALSKPILTLPKSNQKSPLIYARSYVVIDGTSKYPLASKNPDVNVPIAS
                     TTKIMTALVTLDTLPLDQVVTITPEASAIPGSQMNFIKGEKHTVHNLLYALMMNSAND
                     AAVALSQANGTTEEFVSRMNKKAQTLGLANTHYIDPAGLEEGSQSSPRDLALMLDYAL
                     GNKTFRSIVGTQTHTITSVDNRYTYQLNNSNRLIHPDEPLYLPSTIGGKTGFTYEAGH
                     CLIAAYERADGSRLIAAVLHTNSDDNDASAKEARKLLTWANQL"
     gene            complement(1738..2466)
                     /locus_tag="VGE59_02440"
     CDS             complement(1738..2466)
                     /locus_tag="VGE59_02440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY1074537.1"
                     /translation="MTPQNERLNKVLAQLGYGSRRSTDELIEAGRVAINGQQAELGQR
                     VAPNDTITLDGKSIGREKPRTVVLAFHKPRGVTTTKKDKYAKVTVMEYLPKQFQHLFP
                     IGRLDRDSRGLLLFTNDGDLALKLEHPRYEHEKEYVVDVIPNKRCDLSIFKQDMIRMS
                     KEVIDQEHQTKPAQVTQFTYDGKLKRGHARIILKEGKKRQIRHLLQALGYFVSDLQRV
                     RIGSIRLGDIPEGKYVELKPEELL"
     gene            complement(2438..3862)
                     /gene="aspS"
                     /locus_tag="VGE59_02445"
     CDS             complement(2438..3862)
                     /gene="aspS"
                     /locus_tag="VGE59_02445"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY1074538.1"
                     /translation="MQPSAKPRQYIIDSTSKIGETITIAGWVSARRDHGKLIFLDVRD
                     KTGLLQLVLHPQISADAHAAASEVRDEFVVQVTGEVQAREERLINPNLATGTVELAVK
                     DLVIMSRAETLPFSLENTKAINEETRLKYRYLDLRSDRMTSNIRLRHKVKKLARDFLD
                     EAGFVEIETPLLTQTAPEGARDFLVPSRLQPGKFYALPQSPQQYKQLLMIAGFERYYQ
                     IAKCLRDEDLRGDRQPEHTQIDMEMSFMTEQEVRDLTEAMMIHIAEACGKKILQKPFP
                     VFTHEEAMEKFGQDKFDLREEKDPDTLAFAWVVDFPLFEWDETAHRHTFAHNPFSAPK
                     AEHVEKLLKGEDLEHLRAQQYDLVCNGFELASGGVRISDPAIQRKVFEIMGLTEEETE
                     KRFGHLIRAYEYGAPNHAGIAPGLDRFCMLLANEENIREVIAFPVTSSGTTAVMEAPS
                     DASEKHLQELHLKVTHDAPKRTTE"
     gene            complement(3846..>5069)
                     /locus_tag="VGE59_02450"
     CDS             complement(3846..>5069)
                     /locus_tag="VGE59_02450"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074539.1"
                     /translation="IMTESSQRFSRGTDPNLRLPVLKHAQYLLVTLSGGNAFAPVEKQ
                     NHSAAPLKTTLNHSLVTRMMGISLSLESIQNILEALGCQVEAREESIDITPPTWRTDL
                     VIPADYIEEIVRITGLQNIPKQPLPASVPQWKRSKYWRGEWLKDVLVNLGANEIITYP
                     FVSREELDLFGMQSALELRLAPLEGKSLMRPLLLPGALSAVATNPEAPFLALFEVGRV
                     FHPTEEVETLCLILASNSEAENDQWWQNFFERLRVPVSSWMGRVKEVSEDVRQAYKIR
                     KSSVSYIELPLEEIINQKAADIPNVIIPDIDDITYAPLSKFQASRRDIAFIVAKDNTI
                     DTISQDIKQIHPYIIDTELFDTYTNSKIGDNNQSLAYHIVYQAPDKTLTAEEIQTIHS
                     QIETMLKERYHATIR"
BASE COUNT         1226 a         1053 c         1244 g         1546 t
ORIGIN      
        1 tagggacgta ttttcttata ccaagcttcg gcttcaagtc cagcccactt ctttggcata
       61 agttcgacgg gcaaatgcgg gtctttcttt gccacaagtg cgagactgac gatcatacat
      121 ttagctgtaa aggagatatc cttttgtttc ttaagatttt ttaattgagt atcaagctct
      181 ttaaagagtt ctcgatattc tttatcaagt ttatcgagtt gccagagaga cagtacgtca
      241 atagcattgt tgccaaagtg ttttgcttca aagaattgaa cagtatgagc aaattccgtc
      301 tgcataatta aatcataaaa tttgtcagct acacttttat taggggtgat ccatacactc
      361 gattggagca tccgggctcc aaagtctaag agtttaatcc gaaacatttg tcgtacggtt
      421 cgttttgatt cgggaatatc aaaaagaatg atacgccact tcccatccca atttttttca
      481 ctgtcaggaa ggtgacccag aatatcggct aaaacgaggt cccctttttc ggtcaatgta
      541 aagacgttac gtttctcttc ttttgtccgc tcaataagct ttaggccttc aagacgtccg
      601 agtgcaccga gggtttggcg cgtgctccca aattgtttga gatcgtcaag gacaatctcc
      661 cctttgtctt ctgtcaaaac aagaatctta tgaaataacg agaggtcgga tgttcgtgtc
      721 atagcccgat actaacatct tctatgcgtg cgtcaagtaa gcttacggtg gaacaagatg
      781 gttataactg atttgcccaa gttaaaagtt tacgagcttc ttttgcgctt gcatcattgt
      841 catctgaatt agtatgaagc acggcggcaa ttaatcgaga gccatcagct cgttcgtagg
      901 cggcaataag acagtgtcct gcttcatatg taaagcctgt tttgccccca attgttgatg
      961 gtaggtagag aggttcgtca gggtggataa gccgattaga attattgagt tggtacgtat
     1021 atcggttatc tactgatgta attgtgtggg tttgagtgcc aacgatgctg cgaaatgttt
     1081 tgtttccgag ggcgtagtcc aacataaggg ctaagtcgcg agggctcgat tggcttccct
     1141 cttccaggcc ggctggatca atatagtgag tattggctaa tccgagtgtc tgggctttct
     1201 tattcatacg cgaaacaaat tcttcggttg ttccgtttgc ttgagagagg gcaacggcgg
     1261 catcgttggc ggaattcatc attaaggcgt acagcaaatt gtgtacagta tgtttctccc
     1321 cttttataaa attcatttgc gaacctggta tggcagatgc ttcgggtgta atagtaacta
     1381 cttggtcgag tggaagtgtg tctaatgtta ccagcgccgt cattattttt gttgtcgaag
     1441 caatgggaac attcacatca ggatttttcg atgccagagg gtacttggaa gtgccgtcga
     1501 tgacaacata cgatcgagca tatataaggg gagatttttg gttgctcttt ggcaatgtca
     1561 gaattggttt gctgagagcg tcaacagaat gtgctaattc tttatcgatt gtgatgagtg
     1621 tttcgtcatt atttaaagcg gaagctgttt tatgctgtaa aatagtattt cccaacccca
     1681 atatggcgag taccagtagg ataataacag taggtctatg ggtgagaata cgcgaaatca
     1741 taggagctct tcaggtttta actcgacata tttcccctca gggatgtctc caaggcgaat
     1801 actgccgatc cggactcgtt ggagatctga gacaaagtat ccaagggctt gaagcaaatg
     1861 tcttatctga cgctttttgc cttctttaag aataatacgc gcgtgaccac gttttaactt
     1921 accgtcgtag gtaaattgtg ttacctgagc tggcttggtt tgatgctctt ggtcaattac
     1981 ttctttgctc atgcgaatca tatcttgctt gaaaatcgag aggtcacaac gcttattggg
     2041 tatgacgtct acgacgtatt ctttttcgtg ttcgtaacgc ggatgttcaa gtttgagagc
     2101 caggtcgccg tcattggtaa agagcaaaag tcctcgacta tctcgatcca gtcttccaat
     2161 tgggaataaa tgttggaact gtttggggag gtactccatc accgtgactt ttgcatattt
     2221 atcttttttt gttgtggtga cgccccgagg tttatgaaag gctaagacga ccgttcttgg
     2281 cttttctctc ccaatacttt ttccgtcgag agtaattgta tcgtttggtg cgacccgttg
     2341 cccgagttct gcttgttgcc cattaatagc aactcgccca gcttcaatga gctcgtcggt
     2401 tgatcgtcga gagccatacc caagttgggc gagaacttta ttcagtcgtt cgttttgggg
     2461 cgtcatgggt tactttaaga tgaagctctt ggagatgttt ttcgcttgca tcagatggcg
     2521 cttccattac ggcggttgta ccacttgatg ttacggggaa ggcaataact tcacgaatgt
     2581 tttcttcgtt ggcgaggagc atacagaatc ggtcgaggcc gggtgcaatc cctgcatggt
     2641 tcggtgcgcc atattcgtag gcacgtataa gatgaccaaa ccgcttttcg gtttcttctt
     2701 cagtaagacc catgatttca aacactttcc tctgaatggc tggatcagaa atacgtacac
     2761 caccggaagc aagctcaaat ccgttacaga ctaagtcata ctgctgagct cggagatgtt
     2821 ctaagtcttc accctttaaa agtttctcaa cgtgttctgc tttaggtgca gaaaaaggat
     2881 tatgggcaaa ggtgtgccta tgagctgttt cgtcccattc gaagagagga aagtcaacaa
     2941 cccaagcaaa cgcaagagta tcggggtctt tttcttcccg aagatcaaat ttgtcttgtc
     3001 caaatttttc cattgcttct tcgtgcgtaa acacgggaaa tggcttctgt aaaatctttt
     3061 taccgcatgc ttcggcgatg tggatcatca tcgcttcggt gaggtcgcgt acttcctgtt
     3121 cggtcatgaa tgacatttcc atgtcaattt gagtgtgctc tggctgacgg tcgccacgta
     3181 agtcttcatc acgcaggcac ttagcaatct ggtagtatct ttcaaatccg gcaatcatga
     3241 gaagctgttt gtactgctga ggcgactggg gaagcgcata aaacttaccg ggttgtaaac
     3301 gagaaggtac aagaaagtcg cgggcgcctt caggtgcggt ttgtgtcagg agcggcgttt
     3361 ctatttctac aaatccggcc tcgtccaaga aatcacgcgc gagtttttta actttatgtc
     3421 gaagtcggat attgcttgtc atccgatcgc ttcgtaagtc taagtagcgg tatttaaggc
     3481 gggtttcttc gttgatagct ttggtgttct caaggctaaa cggtagtgtc tcagcgcgac
     3541 tcataataac aagatctttt actgccagtt caaccgtacc tgttgcaagg ttggggttaa
     3601 tgagccgttc ttcccgtgct tggacttctc cagtaacttg aaccacaaat tcatcgcgaa
     3661 cttctgatgc ggcggcgtga gcatcggccg aaatctgagg gtgtaatacc agttgcagga
     3721 gtccggtctt gtcgcgtaca tctaaaaaga tgagtttgcc atgatcgcga cgggccgata
     3781 cccatccggc gattgtaatg gtctctccga tttttgaggt tgagtcaata atgtactgac
     3841 gtggtttagc ggatggttgc atggtaacgt tcctttagca tggtttcgat ctgtgagtga
     3901 atggtttgaa tttcttcggc ggtgagagtc ttgtcgggag cttggtagac aatatgatat
     3961 gccagtgatt gattattgtc gccgatctta ctattagtat aagtgtcaaa gagctcggta
     4021 tcaataatat aagggtggat ttgttttata tcttggctaa tggtatcaat tgtattatct
     4081 tttgcgacaa tgaaggcgat gtctcgacgg cttgcctgaa actttgaaag gggtgcatag
     4141 gtgatatcat caatgtccgg gataataaca ttcggtatgt cggcggcttt ctgattgata
     4201 atttcctcga gtggtaattc aatgtagctc acggatgatt tccgtatctt ataggcctgt
     4261 cggacgtctt cgcttacttc ttttacacgt cccatccatg aagatacggg cacgcggagg
     4321 cgctcaaaga agttttgcca ccattggtcg ttctctgctt cactgttgct cgcaagaata
     4381 agacagaggg tttctacttc ttcagtaggg tgaaatacac gacctacttc aaaaagggca
     4441 agaaatgggg cttccggatt tgttgcaaca gcagagagag cacctggtag tagcagcggg
     4501 cgcatgagtg acttgccttc aaggggagca agtcgtagtt cgagcgctga ttgcatgcca
     4561 aataaatcga gctcttcgcg gcttacaaaa ggatacgtaa taatttcgtt ggcgcctaaa
     4621 ttgactaaaa cgtctttgag ccactcccct cgccaatatt tcgaccgttt ccattggggg
     4681 acactcgccg gaagcggctg tttgggaatg ttttgtagtc cggttatacg tacaatctct
     4741 tcaatatagt cagctgggat tacaaggtcg gtacgccacg ttggaggcgt tatgtcgata
     4801 ctctcctctc tggcttcgac ctgacatccg agcgcttcga gaatattctg gattgattcc
     4861 agtgatagtg atatgcccat catccgagtg actagggaat ggttcagggt cgtctttaac
     4921 ggtgccgcac tgtggttttg tttttcaact ggggcaaaag cattgccccc gctaagtgtt
     4981 actaatagat actgggcgtg ttttaaaacc ggtaatcgta aattcgggtc agtgccacgg
     5041 ctgaaacgct ggctactctc cgtcataat
//
LOCUS       DASWYB010000042         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_79147, whole genome shotgun sequence.
ACCESSION   DASWYB010000042 DASWYB010000000
VERSION     DASWYB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79147"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGE59_02455"
     CDS             <1..545
                     /locus_tag="VGE59_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY1074540.1"
                     /translation="VPLKRVVLVGVAILVIIVGLNVTKLPKWQTSITSSRESRLEYWD
                     IALHVIRDNFWTGIGIKTWETDYVHLIEKYRTDPPINWVSPQPHNVYLDAFVKAGLPG
                     FFAIMAFLLWPVAEGFAYAKSYASKNPTWWYGLSLFGVGIALLIFGLIDDPLWSDDVM
                     PLFAIFFGFLAYLAARKNTA"
     gene            complement(542..1348)
                     /locus_tag="VGE59_02460"
     CDS             complement(542..1348)
                     /locus_tag="VGE59_02460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1074541.1"
                     /translation="MTHRPDLSVIIVSYNTRDLTIQTVGTLLKSTKKPLEIIVVDNAS
                     SDGSADALRAAFPEIRVFAETKNLGFGKANNLGAHHATAEILYFLNSDVIMNPTSVDP
                     LYDYLIEHPKAGIIAPKLLLEDGKTMQQAAFGQEPTLMNLLLRQQQRHDSSHTDSQTH
                     VVDWVTGAAFLMRRDLFLVVGGFDEALFMYYEDIDLCHRVRDAGYSIIWNPSATLVHL
                     GGKSSISTWRRKRLYYASQIYYMKKHYGSLIATILWCARLPLVVYYRLKG"
     gene            complement(1345..2250)
                     /locus_tag="VGE59_02465"
     CDS             complement(1345..2250)
                     /locus_tag="VGE59_02465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1074542.1"
                     /translation="MSKATPPKVVTILINWNGKEDTLRCLESLTKSNYPTNHTVVVDN
                     ASSDDSLAVIKKQFPEITLLPQSKNLGFSGGNNKGMVWALEHDADYCFILNNDTTVEP
                     NTITRLVETFQTNTHIGAVGPLIRYMEPKDTIAALGGTIRWNKAEAIQGYNQEPVTNA
                     PKEPFETDFLTAAAVLVSKQAIEKAGMLPEGYFYGMEDAAWCVHIKRAGFTLVADPCT
                     AIDHKESAATGQSSPIKAYYQSRNALLFLKKEWPEKWPYFLLTYHIKVIKTTVKWILQ
                     GKWSWLKGFWQGYSAFWRGHEGQRI"
     gene            complement(2243..>3212)
                     /locus_tag="VGE59_02470"
     CDS             complement(2243..>3212)
                     /locus_tag="VGE59_02470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY1074543.1"
                     /translation="HTPPKTGKEWIKGPAKALLNAWGRVSGRKEITWMPVRVPLRAVR
                     KLSDQYLPDADLLIATENQTIDPLDLVSDKKGKKIYFIQHYETWSRAEKEVDATWKSP
                     KWHKVTIATWLVNLAKEKFHSHADLVVNGVDISVFHPKGRKTHTPPRVLSLYHHLEWK
                     GIKDTIQAMAAVQKKGIPFTPVFFGHLPAGSDLDVLEGVEYHLFPHGETLRQLYASAE
                     IFVSASWAEGCQLPPMEAMACKAALIASNVGGVPDYTIPGKTALVVEPKHPGDLAAAL
                     EDLLLHPEKRTKIADAGYDHIQKFSWENVADTFETLLQNIHQGTHV"
BASE COUNT          749 a          698 c          841 g          924 t
ORIGIN      
        1 ttgtacctct taaacgggtc gtcttagtgg gcgttgctat tcttgttatt atcgttggct
       61 taaatgttac caaattgccg aaatggcaaa cgagcatcac ctcaagtcgc gagtctcggc
      121 ttgaatattg ggatattgct ctccatgtta ttcgcgacaa tttttggacc gggattggca
      181 taaaaacatg ggagacggac tatgttcatc tgatcgagaa gtatcgaacc gacccgccaa
      241 ttaactgggt gtcaccgcaa cctcataatg tttaccttga tgcttttgtg aaagcagggt
      301 tgcctggatt ctttgcaatt atggcctttc ttctctggcc ggtagcggaa ggttttgcct
      361 atgccaagag ctatgcctca aagaatccta cgtggtggta tggcctcagc ctttttggtg
      421 ttggcattgc ccttttgata tttgggttga ttgatgatcc gctctggagc gatgacgtga
      481 tgccgctctt tgccatcttt tttggatttc tcgcatactt ggctgcgaga aagaatacag
      541 cttaaccttt gagtcgataa tacacgacaa gggggaggcg agcacaccag agaatggtag
      601 ctataaggga accataatgt tttttcatgt agtaaatttg tgaagcataa taaagccgtt
      661 ttcttcgcca tgttgaaatg ctgctttttc caccgaggtg aactaacgtt gcgctgggat
      721 tccatataat tgagtagccc gcgtcgcgta cccggtgaca gagatcaatg tcttcgtagt
      781 acataaagag ggcttcatca aagccgccga cgacaagaaa caagtcgcgc cgcatcagaa
      841 aggcggcacc agtaacccaa tcgacgacgt gagtctgtga gtctgtgtga gaactatcgt
      901 ggcgctgctg ttgccgaagg agtaagttca taagcgttgg ttcttgccca aaggctgctt
      961 gctgcatagt cttgccatct tcaagtaaga gtttgggggc gataattccg gctttcgggt
     1021 gctcgatgag atagtcatag agtggatcga ccgatgtggg attcataatg acgtcgctat
     1081 taaggaagta aagaatctca gccgtggcat ggtgtgcgcc aaggttgttg gcttttccaa
     1141 acccaaggtt ttttgtttcg gcaaaaactc ggatttcagg aaatgcagcg cgtaatgcgt
     1201 cggccgatcc atcgcttgaa gcgttatcaa ccacaataat ctcaagtggt ttctttgtac
     1261 tctttaagag cgttccgact gtttggattg ttaagtcgcg tgtgttatac gagacaataa
     1321 tgaccgatag atcggggcgg tgtgtcatat gcgttgtcct tcatgtccac gccaaaaggc
     1381 gctatatcct tgccaaaaac ccttcagcca ggaccatttt ccttgaagga tccatttcac
     1441 agtcgttttg atcactttaa tatggtacgt caggagaaaa tacggccatt tctcgggcca
     1501 ctcttttttt aaaaagagga gtgcgttgcg tgattggtag taggctttaa taggggagga
     1561 ttgtccggtc gccgctgact ctttatggtc tatagcagtg cacgggtcgg cgacaagggt
     1621 aaacccagcc cgtttgatgt gaacgcacca cgctgcgtct tccatcccgt aaaagtaacc
     1681 ttctggcagc attccggctt tctcaatcgc ttgtttcgat acgagaactg ccgcggccgt
     1741 caggaagtcc gtttcaaacg gttccttggg agcgttcgtg accggttctt gattatagcc
     1801 ttggatggct tcggctttat tccaccggat cgttcctcct agagcggcaa tagtgtcttt
     1861 cggttccata taacgaataa ggggtccaac ggcaccgata tgggtgtttg tttggaaagt
     1921 ttcaaccaaa cgtgtgatgg tgttcggctc tacggttgta tcgttgttga gaataaagca
     1981 gtagtcggca tcgtgctcga gagcccaaac cattccctta ttgtttccgc cagaaaaacc
     2041 cagattttta gattgaggta gcaaagtgat ttctggaaac tgttttttta taacggcgag
     2101 agaatcatcg cttgaagcgt tatcaaccac aaccgtgtga ttagttgggt agttactttt
     2161 cgtgagtgac tcgaggcagc ggagggtatc ctctttgcca ttccagttga ttaagatggt
     2221 cactacctta ggaggtgtcg ctttagacat gggttccttg gtgaatattc tgcaatagtg
     2281 tctcaaaggt gtcggctaca ttttcccaag aaaatttttg aatatggtcg tagccagcat
     2341 cggctatctt ggtccgcttc tcggggtgga gcagcagatc ttctaaggcg gcagcgaggt
     2401 ctcccgggtg ctttggctcg acaacgagag cggttttccc tggaattgtg tagtcaggca
     2461 cccctccgac gttactggca ataagggcgg ctttgcaggc catggcttcc atgggaggaa
     2521 gttggcaccc ttcggcccaa ctggctgaga caaaaatttc agccgaggca tacagttggc
     2581 ggagtgtttc tccgtgagga aagagatggt actcaactcc ttccaataca tctaaatcag
     2641 aaccagcggg taagtggcca aagaagacgg gagtaaacgg gatgcctttc ttctggacag
     2701 cggccatagc ttggatggta tctttaatac ctttccattc gagatggtgg tagagagaaa
     2761 ggactcgagg aggggtatgg gtttttcggc ctttgggatg aaaaacagat atatcaaccc
     2821 cgttgacgac taaatcagca tgggagtgaa acttctcttt ggctaggttt acgagccaag
     2881 tggcaatagt aaccttgtgc cacttgggcg atttccaggt cgcatcgact tctttttccg
     2941 cgcggctcca ggtctcatag tgctgaatga agtagatttt tttacctttt ttatcggaaa
     3001 ctaaatcgag tgggtcaatc gtttgatttt cagtggcaat aaggagatct gcatcgggga
     3061 ggtactgatc gcttaatttt cgtactgccc gcagggggac tcgtactggc atccaggtga
     3121 tttctttccg gccacttact ctgccccatg cgttaaggag tgctttagca ggacctttta
     3181 tccactcttt ccctgttttt ggaggagtat gg
//
LOCUS       DASWYB010000043         6335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_894699, whole genome shotgun sequence.
ACCESSION   DASWYB010000043 DASWYB010000000
VERSION     DASWYB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894699"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VGE59_02475"
     CDS             <1..318
                     /locus_tag="VGE59_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY1074544.1"
                     /translation="YDATSGGKWRFVSHDQHGNEFAFHGCIHEVNPNTRLIQTFEFEG
                     LSESGHVALERAEFASKGDNETEIRITSTFQSVEDRDGMVASGMESGFRKSIDALDAL
                     LVS"
     gene            complement(402..701)
                     /locus_tag="VGE59_02480"
     CDS             complement(402..701)
                     /locus_tag="VGE59_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074545.1"
                     /translation="MTHENCNKAFELIGDRWTLSIFYVLKKQDERFCALQRELNLNPI
                     TLTKRLKKLEEFKLIKRIEGTEDKKSVTYSLTPLGREFLPIVDSIEAFGIRLKQI"
     gene            805..1746
                     /locus_tag="VGE59_02485"
     CDS             805..1746
                     /locus_tag="VGE59_02485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY1074546.1"
                     /translation="MKAAQINQYGGPEVIQVNETDKPEVKEGYVLIEVHAASLNPFDT
                     TARSGQAHARMPLHFPATLGGDVAGVVTEISEGTTGIAVGDEVYGQASVFAGGTGSFA
                     EFTATQPGVIAKKPETLHFNEAASLPLVGSSAIQALMEHLKLQKGQTILIHGGAGGIG
                     SLAVQLAKHLGAHVIATAATNDIEFVKGLGADEVVDYQNEKFEDKVSDVDAVFDTVAG
                     ETYTRSFPILKKGGTIVSMLEQPNQELMEKYGVTAIAQFTTITTEKLNTLSKLVDSGV
                     IKPQVDKVFELNKVTEAFMYREHQHPRGKVVIEVKGK"
     gene            complement(1887..2465)
                     /locus_tag="VGE59_02490"
     CDS             complement(1887..2465)
                     /locus_tag="VGE59_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY1074547.1"
                     /translation="METFKDLPVLLFTTQFEWGNWLESNHNQPQGVWLKHAKKSSGKT
                     SVSYAEALEEALCYGWIDSQKQAYDNDYYLQKYSPRSSHSVWSKINVAKVEALIKSEK
                     MQPAGLAAIDVAKQTGRWDAAYDSQSVSTIPEDFLQELNKDPKAKKFFETLNKANVYA
                     FCWRIQTAKKPETRKARIEKFIDMLNRGEKLH"
     gene            complement(2662..3552)
                     /locus_tag="VGE59_02495"
     CDS             complement(2662..3552)
                     /locus_tag="VGE59_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074548.1"
                     /translation="MKDGMRQVSLEELAAEFSVPAVEDPQIAAVEEVPRVTPSNRKLP
                     ADPRRQFRKAGEQIPQNLWPTCPDGMLATPVHTFLPMFEGEVWAGSKSSPRDTHIAFP
                     ANCFPHRDPMSQHSALLSATAREHGWHVVTGLAPNDLPSYTGGYTLTVPHKGKCLIPP
                     PGLWLHTLPQQVRSGEVDVSEDNGWFDIFDVNVGYLAWSDNATLARQGWQHGDGYHYV
                     TIPAKGKWWFHGSLPGLRELLEKMYPEQHRVEADVSRATLYLEVPKTDVGRWIGCEGR
                     MVKNLSQWIGWPTIRISAFR"
     gene            complement(3815..4042)
                     /locus_tag="VGE59_02500"
     CDS             complement(3815..4042)
                     /locus_tag="VGE59_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074549.1"
                     /translation="MNTMLKGIAVLPHSAGMFTANEVRVQGETKEELLAHAWQECVGS
                     RYVSSIYDATGTIELYSEAKGWLMEPTPEPE"
     gene            complement(4316..4714)
                     /locus_tag="VGE59_02505"
     CDS             complement(4316..4714)
                     /locus_tag="VGE59_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074550.1"
                     /translation="MPFFGDETTRELMLLAVRSAPEGMDALTAYDLVADGVIIMKKPD
                     HVEVNVVAGHWVLSMLEVKKAFKWLLRHGLIEPTPWYKVVVSNLRDTDKFRITPEGEN
                     ELIKWYTILARFGTGPLYKAGEKPEVDPES"
     gene            complement(4990..5064)
                     /locus_tag="VGE59_02510"
     tRNA            complement(4990..5064)
                     /locus_tag="VGE59_02510"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5030..5032),aa:Lys,seq:ctt)
     gene            complement(5104..5179)
                     /locus_tag="VGE59_02515"
     tRNA            complement(5104..5179)
                     /locus_tag="VGE59_02515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5144..5146),aa:Arg,seq:tcg)
     gene            5449..>6335
                     /locus_tag="VGE59_02520"
     CDS             5449..>6335
                     /locus_tag="VGE59_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY1074551.1"
                     /translation="MKKHQTNSSHRTKLALAGVALIAAIVFYVIGNNSPQAAVGNIAS
                     VSVSKGVVTFPANTNVSQFQFHVYQVINGTRALITSQPSFGLSAAADPTISAPNRTFS
                     TLATATNPSGAFQVRNGGTYDIEIKHPSLINAATGFGCFARFIVPNSGTSFVLTDGQF
                     LTGSTRLLDRISFPCSGTPTPTSTSTSTASATPVTTGTLPAICSKPKLDVMLVMDNSG
                     SMNNPSNNGPVSSVLMKDMIGAFINDGQGVSKYQAGDRIGGIRFAWDGNNDLDKTHTS
                     DSFAASKAIMQLSGSTITDT"
BASE COUNT         1695 a         1565 c         1386 g         1689 t
ORIGIN      
        1 tacgatgcaa cctcgggtgg taagtggcga tttgtaagcc acgatcaaca cggtaacgag
       61 tttgccttcc atggctgcat tcacgaagtg aatccgaata cccgtctcat tcaaactttc
      121 gaatttgaag gactctccga aagtggacat gttgcactcg agcgtgcaga gtttgcttca
      181 aagggtgata acgaaaccga aatccgtatc acttctacct tccaaagtgt tgaagaccga
      241 gatggcatgg tggctagcgg catggagtca ggcttccgca aatcgattga tgccctggat
      301 gcattgttgg tcagctaatt ttggctcaat agaatcaata aacgacgaag gtctccttcg
      361 tcgtttattg aaataacctt acaaagatta ttatggaaaa cttagatttg cttcagcctg
      421 ataccaaaag cctcaattga atccactata ggcaaaaatt cacgccccag gggagttaaa
      481 ctataggtaa ctgacttttt atcttcagtg ccttcaatcc gtttaataag tttaaactct
      541 tctaactttt ttaaccgttt agtgagagtt atagggttaa gattaagttc gcgctgaagg
      601 gcacaaaatc gctcatcttg ttttttaagc acgtaaaaaa tagagagtgt ccaacggtca
      661 ccaataagtt caaatgcttt gttacaattt tcgtgggtca tacccatagt actattgctc
      721 tattatttat actcatgtat tttgatatta ctatagaaag tatagcaata aagatcaaag
      781 gtcgctagag ccagaaagga acgtatgaaa gcagcacaga tcaaccagta cggaggtcca
      841 gaagttattc aggtcaacga aaccgataag ccggaagtaa aagaaggata cgtacttatc
      901 gaagttcatg ccgcaagtct caatccgttt gatacaacag cccgaagcgg acaagcacac
      961 gccagaatgc cgctccattt tcctgccacc ctcggcggcg atgtcgccgg cgttgtaacc
     1021 gaaataagcg aaggtacgac tggaatcgca gtgggagatg aggtctacgg acaagcgagc
     1081 gtttttgcag gcggaaccgg aagttttgct gaatttaccg caactcaacc cggggtcatc
     1141 gcgaaaaagc ccgagacgtt acattttaac gaagcagctt ccctccctct tgtaggaagt
     1201 agcgccatcc aggccttaat ggaacattta aaactccaga agggacagac aatccttatt
     1261 cacggcggcg cgggtggaat tggttctctt gcagttcagt tagcaaaaca cttaggcgcc
     1321 cacgtgattg cgaccgcagc aaccaacgac atcgaattcg taaaaggcct tggcgcagat
     1381 gaagtcgttg actatcaaaa cgaaaaattc gaagacaaag tttctgatgt cgatgctgta
     1441 ttcgacacag tggcaggaga gacctatacg cgttctttcc ccattctcaa aaaaggtggc
     1501 actatcgtat cgatgcttga gcaacccaat caagaactaa tggaaaagta cggcgttacc
     1561 gccatcgctc aatttactac aattacaacc gagaagctca atactctctc aaaactcgtt
     1621 gatagtggcg taatcaaacc tcaggtcgac aaagtcttcg aactgaataa agttaccgaa
     1681 gctttcatgt atcgtgaaca ccaacaccct cggggaaagg ttgtgattga ggttaagggc
     1741 aagtaggaaa taactttcct cagctattcc acaaacgacc cgccaacctc ggcgggccgt
     1801 ttgtggaata caatataaca gattaggttt ttattatttt catagagaac cagggtagaa
     1861 attctcgcaa ctcagactaa atattgctaa tgtaacttct caccacgatt taacatatcg
     1921 ataaattttt ctattcttgc cttcctagtc tctggctttt tagcggtctg aatccgccag
     1981 caaaaagcat acacattggc tttattaagc gtctcaaaaa actttttcgc ttttggatct
     2041 ttattaagtt cttgtagaaa atcttctgga attgtactta cgctttgtga gtcatacgca
     2101 gcatcccatc tgccagtctg tttagctaca tctatagctg ccagtccggc tggctgcatt
     2161 ttttccgact taatcagcgc ctctactttt gcgacattaa tttttgacca aacactatga
     2221 gagctcctgg gcgaatactt ctgtagataa taatcattat cgtatgcttg cttttgacta
     2281 tctatccacc cgtagcacaa ggcctcctcc agagcttctg cgtagctcac tgacgtttta
     2341 ccagatgact tcttcgcgtg cttaagccat acaccttgtg gctggttatg attcgattca
     2401 agccaattcc cccattcaaa ttgcgtggtg aataataaaa ccgggagatc tttgaatgtt
     2461 tccataatca aatgatatca ataaaaaact ctcccaaata gagaagatta atctggctgg
     2521 ggagaagagt ggttgattat gaacatcgag tgaatttact cgtgagttca ttaagtgtat
     2581 tcaaggtggt cggcgtataa aaatagaaag cccccgcgac agcgaggcca cgcggacctt
     2641 ctgtgcgggg gagcaactta cctagcgaaa cgcggagatc cggatagttg gccatccgat
     2701 ccactgcgaa aggttcttca ccattcgccc ttcgcatccg atccatctcc ctacatctgt
     2761 tttcggtact tccagatata gggtagctcg agatacatca gcctcgactc gatgctgctc
     2821 tgggtacatc ttttccagca gctctcgcaa tcccggcagt gacccatgga accaccactt
     2881 tcccttagca gggatagtaa cgtagtggta cccgtcacca tgctgccacc cctgacgagc
     2941 aagtgtggcg ttgtccgacc aggcgagata tcccacgttg acgtcgaaga tatcgaacca
     3001 gccattatcc tccgacacat ccacctcccc gctccggacc tgctgcggca gcgtatgcaa
     3061 ccacaagcct gggggaggga tcaggcactt gcccttgtgg ggtacggtca gtgtgtatcc
     3121 gcccgtgtag ctcggcaggt cgttaggggc taggcccgtt acgacatgcc aaccgtgttc
     3181 tcgagccgta gctgacagca gtgccgagtg ctggctcatg ggatcgcgat gggggaagca
     3241 gttcgccgga aacgcgatat gcgtatcacg ggggctagac ttacttcctg cccatacttc
     3301 cccttcaaac atcggcagaa acgtgtgaac cggcgtcgcc aacattccgt cgggacacgt
     3361 cggccaaagg ttctgcggaa tctgttctcc ggctttccgg aattgacgac gcgggtcagc
     3421 gggaagcttc cgattactcg gagtgacccg agggacttct tcgacagccg ctatctgagg
     3481 atcttccaca gctggaacac tgaactctgc ggcaagttcc tctaggctta cctgcctcat
     3541 cccatctttc attaggattt cctctagcga agcagaggca caataggtgc atgctcagtc
     3601 gtaagatata tatcacaaat gggttaatat gtcaactgta ttaaacgaaa ataacaggcg
     3661 tttaacctgt tatttccatg gaggaccagg cgttacgtta aatgaacctt tgctggagtt
     3721 tcttgtcgag tttgtcagat gtataaaact aaactttgtg taaaaacaga aagcccccgc
     3781 agtggggcca cgcgggcctt ctgtcggggg acatttactc tggctctggg gtaggttcca
     3841 taagccaccc tttggcttct gagtatagtt cgatggttcc ggttgcgtcg tatatagagc
     3901 tgacatatcg gctcccaacg cattcttgcc acgcatgtgc aaggagttct tccttcgttt
     3961 ctccctgaac tcttacctcg ttcgcagtaa acattccggc agaatgtggc aataccgcaa
     4021 ttcctttgag catagtattc atctccgcga gcttcgctct caacacaact tacagtagca
     4081 caaagatata ctttagtcaa tcttttaagg ttcataaaaa caaaaaaaca ataggcgttt
     4141 aacctgttat tctcatagag agccaaggag gatagaatat gaacttttga ctcaattcat
     4201 taatgagttc gttagttgta taaaacttag tttcgcgtaa ataaaaggcg gtccctactt
     4261 cccacaaggg gttcggaacc gccaatgata gcgtagtgat tcactaaaaa gcctattaac
     4321 tctctggatc cacttcgggc ttctcccctg ccttatacag tgggccagta ccaaaccgcg
     4381 cgaggattgt gtaccactta atcagctcgt tctcaccctc tggcgtgatt cggaatttgt
     4441 ccgtatcacg gaggttactg accacaactt tataccacgg cgtcggttcg ataaggccat
     4501 gtcgcaaaag ccacttgaaa gcctttttga cctccagcat tgacagcacc caatggccag
     4561 caacaacgtt tacttccaca tgatcaggct tcttcattat gattacgccg tcggcaacta
     4621 ggtcgtaagc tgtgagagca tccattcctt cgggtgctga acgcacggca agaagcatca
     4681 actctcgggt tgtctcatca ccaaaaaacg gcacgatctt ctcctccccg atcagacttt
     4741 gctcgtgtaa tagtactatt aataaatacc tttgtcaatc gtgtaaaaac aaaaatgaca
     4801 gcgtttaacc tgttgtttta atggaggacc aagtagtgta cgtcgccaac atggggatca
     4861 tgtatgtcgg cttgggtata attcccgccg ctgcgctctg ggcttgcagg aattatcgaa
     4921 cccacgcggc ttcttttact cacttagtct atccatgcaa aaacccacct tttcaggtgg
     4981 gcttctgcat ggagggccaa gtgggattcg aacccacgac caattgctta agaggcaact
     5041 gctctaccgc tgagctattg gcccatgatt tagatttacc aacaataatg tactaaaaac
     5101 ttatggcgcg cccggaggga ttcgaacccc cggccttctg gttcgaagcc agacactcta
     5161 tccgctgagc tacgggcgca gatcgagaag cactctattt cagtaaggta ttgagttaca
     5221 aatatgtact gcgtcagctc aggaatagag tgacggttta tatctgtctt ggccgttgaa
     5281 ctacggacgc atactcgaaa gacacctctc agtatagcat tttcactctg ttgcagcaag
     5341 aacagtgccg tagaattggc aaaattcaca gagaaagctc tcaagacctt gctcgccttg
     5401 cacagagctg ctaatgttga ttatagttga taggagagta ggagggacgt gaaaaagcat
     5461 caaacaaatt catctcatcg cacaaagtta gcactcgctg gagtcgcgct tatcgctgcg
     5521 attgtttttt atgttatcgg aaataatagt ccgcaagctg cggtaggtaa tattgcttct
     5581 gtttcagtat caaaaggtgt tgtaaccttc cctgcgaaca caaacgtttc tcaatttcag
     5641 tttcacgtct atcaagttat taacggtacc cgagctctca taacctctca accttctttt
     5701 ggtctcagcg ctgctgctga ccctactatc tcggctccta accgaacttt ttctacccta
     5761 gctacagcaa caaacccatc cggggccttc caagtccgta atggcggtac ctatgacatt
     5821 gaaattaaac atcccagttt aattaatgcc gccacaggat ttggttgttt cgcacgtttt
     5881 attgtcccta actctggaac atcttttgtt ctcaccgatg gacagttctt aactggttca
     5941 actcgtcttc ttgaccggat ttctttccct tgttccggta cccccactcc aacttcaacc
     6001 tctacttcaa cagcttctgc cacgccagta acaaccggca ccttgcctgc tatttgtagc
     6061 aagccgaaac ttgatgtcat gctcgttatg gataactctg gcagcatgaa caacccttca
     6121 aacaatggcc ccgtttcatc tgtccttatg aaagacatga tcggtgcatt cattaacgac
     6181 ggccaagggg taagcaaata ccaagctggc gatcgtattg gtggtattcg gtttgcctgg
     6241 gatggaaaca atgacttgga caaaactcac acatccgaca gttttgcagc atcaaaagcc
     6301 ataatgcaac tttccggttc aaccattacc gacac
//
LOCUS       DASWYB010000044        20533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_696726, whole genome shotgun sequence.
ACCESSION   DASWYB010000044 DASWYB010000000
VERSION     DASWYB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20533
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696726"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1469
                     /gene="hisS"
                     /locus_tag="VGE59_02525"
     CDS             219..1469
                     /gene="hisS"
                     /locus_tag="VGE59_02525"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY1074552.1"
                     /translation="MPIQALRGFRDFYPEDQATITYLREKIAASCAKFGYEEFEGPML
                     ESIDLYAAKSSEEIVNEQAFTLEDRGGDRITLRPELTPTLARMVAAKQQQLTFPLRWW
                     CFGRFWRYERPQKGRGREFYQWNLDLLGQNTADADAEIIEISIDFLQSVGVTSDDVVF
                     EINDRSFAEQLLRENNIDTEKTSAVFQLVDRLAKLNSEEKLAFGLARGLSESDVTKIT
                     ELFSAESTAWEQSERIQALLTLFENKGLSSWVRPNLSIVRGFTYYTGLVFEVTVKTGQ
                     SRALLGGGRYSNLVSDMGGQPVEGVGMGMGDMSIAIFLEEKGLLPEYTTSTKACVIGL
                     GPDTAAFCQEVATTLRNATIPTILYGTPENFGKGLKFAGNKKIPYALLIGPDELLNKT
                     VTLKNLETGEQETSSLETILEKLT"
     gene            1563..1817
                     /locus_tag="VGE59_02530"
     CDS             1563..1817
                     /locus_tag="VGE59_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074553.1"
                     /translation="MAAAVAEPTRVDAPPTAGELRMFELEARREALERELEDINRELI
                     ELRQAADLCETCGGTKKVRVRGGLYGELTERPCACAETKK"
     gene            2178..2789
                     /gene="lexA"
                     /locus_tag="VGE59_02535"
     CDS             2178..2789
                     /gene="lexA"
                     /locus_tag="VGE59_02535"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY1074554.1"
                     /translation="MLPLTPRQREILDYITGFVDSNDYSPSYREIAEHFNLSSTATIA
                     EHIETLKTKGYLRDDLSGVRSLQIQAPEPRQEDLLHEMAIPLLGSIAAGLPIEAIRTN
                     ETIDIPRDMAGKKVFALKVRGDSMIEDGIMEGDYVIIEACDNPRNGDIVVALLDRDNV
                     TLKRFYREKDHVRLQPANKKYKPIRVKKVVIQGRVKGVIRKFD"
     gene            2942..3178
                     /locus_tag="VGE59_02540"
     CDS             2942..3178
                     /locus_tag="VGE59_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY1074555.1"
                     /translation="MRKEYKRTLIKFSNYSLCVTLPKAMLKKLELDKGSEVLITLKRG
                     KLIIEPATANGEAASTDQDNDNWEQMQEINEDEE"
     gene            3283..3489
                     /locus_tag="VGE59_02545"
     CDS             3283..3489
                     /locus_tag="VGE59_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074556.1"
                     /translation="MKKHIDIVRKTKRQTMDEAVLKPTTATETPEPALKPGEVKTSPV
                     IVGLLVIVIGIMVGFVFYLFTIQP"
     gene            3592..4152
                     /locus_tag="VGE59_02550"
     CDS             3592..4152
                     /locus_tag="VGE59_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006884962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY1074557.1"
                     /translation="MEYQHQRVAVFVDVQNLYHSAKNLYSANVNFSQILQDAVGNRQL
                     VRAIAYVVRAEGPKEQAFFDALEKAGFELKVKDIQTFAGGAKKADWDVGMAIDALTIA
                     KHVDVVILMTGDGDFTPLISYLQNSEGNLVEVAAFGRSSSSRLVEWADKFLDFENNPE
                     RYLLGYRSPEKAEKPTSAETTEEAIG"
     gene            4247..4633
                     /locus_tag="VGE59_02555"
     CDS             4247..4633
                     /locus_tag="VGE59_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY1074558.1"
                     /translation="MTTLTTPNGTNIGVEIANNGLERTRGLSNRDSLPINRGMLFLFP
                     TSDIYSFWMKDTKIPLDLLWIQDTTIVDITTLPAEIPGETIPQYQPKAEANRVLELNS
                     GEAAKHGLKIGSHIIIDKTCLPPASI"
     gene            4637..5233
                     /locus_tag="VGE59_02560"
     CDS             4637..5233
                     /locus_tag="VGE59_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074559.1"
                     /translation="MSLAYLFGLIILMGLLSNWLSKALATMAPYSIFRLALLPGIILH
                     ESAHVAGCWLTGARVHRVHFWTQSGGEVIHTQPKIPVIGPPIISFAPFVVGMYALWHL
                     GIYLTVPPLEISWQRGLAVFLMMSIGATLIPSKQDFKNSFGSLVVIAILASAALYFFP
                     SLWHPLNTYAQPLLKPFILVTAIIATLTFLANLTMWRR"
     gene            5639..6979
                     /gene="xseA"
                     /locus_tag="VGE59_02565"
     CDS             5639..6979
                     /gene="xseA"
                     /locus_tag="VGE59_02565"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY1074560.1"
                     /translation="MQSLFSDPSKIIYTVKEFNELVNIVLTEKVGEVSIQGEITGYQI
                     RQGKWISFDLKDSESVINCFATTYQIDMPLSDGMEVVVTGTPRLYVPYGKYSFNVRNV
                     QLKGEGALKKAFEDLKKKLTTEGLFDPIHKRSLPRFPEKIGIITSGEGAAVNDILKVI
                     NGRWGGLEIYLAPVLVQGKNAPDELVGAIQYFNQRFPVDVVIFGRGGGSLEDLQAFNS
                     ERVARAIFASRIPIVAGIGHEHNTSIADLVADVRAATPSNAAEICVPDRAAIAREVEF
                     LSHKAERLVQQRIMSHRHHLNNQTQTLELFVSTRFARIRSLITRVPQLLHSYQEKTRL
                     IHQKIAMSHTHLSQRMNDRFETSLRQLKEKEKLLAALNPTAILKRGYSITYATGTGTP
                     ITRLADIPADGRIRTALSDGIIESHISEAGREIKKSPPAKTVKITLEKAQETLF"
     gene            6995..7219
                     /gene="xseB"
                     /locus_tag="VGE59_02570"
     CDS             6995..7219
                     /gene="xseB"
                     /locus_tag="VGE59_02570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY1074561.1"
                     /translation="MQKEQKFEEIVTELETIVSELDSSIGLEESLKKFERGMELAKHA
                     EERLKTIENKFQKIQQQFVPEAEVIEQTEE"
     gene            7371..8288
                     /locus_tag="VGE59_02575"
     CDS             7371..8288
                     /locus_tag="VGE59_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074562.1"
                     /translation="MNREWQKICVICLTVPDIGTPQAPTEGDAVITVQTQIAAHMMKT
                     VGVELGTYTRLAAAIGPDTRPMLLPKAMYEGPTPERPNSGPPPSGRWAHLQGRYPILR
                     SRPESPCLLNPPPENSIPLQIMTAFLNGVGQSDCPIYEDNVFALECRDNALGKSFGAM
                     LEPALRQSVGDRIMRQLRKHYTPGVSGLMGDPSLIYHLGQQRIKLWTYIKYVAELIDD
                     RQVDGFDPGSNFAILLVLQHPFAIEQIWHTLVGERQVPPGGAMLNTYHRKVVEGEDYM
                     YLAKAARVYPPSIGRRPVIVGFPPPPLLR"
     gene            8358..8756
                     /locus_tag="VGE59_02580"
     CDS             8358..8756
                     /locus_tag="VGE59_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074563.1"
                     /translation="MHKIFSRIVIGLLILFGLYIMLGNLDSLLDYQDFKMIIVYGLDL
                     AKDAIHIYLGFLAWLISVLFFHRSPRTPQVLVLGFVFSLLMELADIRGDWIQYRSPDW
                     MGSLHDIVHTNMIPTLLFIFFRTYKEEEIK"
     gene            8753..9160
                     /locus_tag="VGE59_02585"
     CDS             8753..9160
                     /locus_tag="VGE59_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074564.1"
                     /translation="MNHRVVASAVIYKDNHILIAKRAATKKVFPNAWEIPGGKVEVGE
                     SPQQALKREVKEELGIDIEVMQPLCVYSSQIGNEWWTEIDFVAVLLLPSQTITLNTHD
                     HSDYAWINQSQIDEYFDETDNNGRAVRQAFQEL"
     gene            9157..9834
                     /locus_tag="VGE59_02590"
     CDS             9157..9834
                     /locus_tag="VGE59_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY1074565.1"
                     /translation="MTLIDEILTDLKSLESEKNRAGMARFGIQVEHAYGVSVANIRRL
                     AKPYRHNHELALELWKTGIHEARILAAIIDDPKQVTPEQMDRWVQNFNSWDLCDQTCS
                     LFDKTPHAVAKIKEWTTREKEFEKRAGFALLAELAWHSKTEPDQTFLDLLPLIEREAH
                     DDRNYVKKAANWALREIGKRSMRLHAEAIKLAQRLQTHDSKSARWIASDALRELTAEK
                     TLSRINK"
     gene            complement(9839..11107)
                     /locus_tag="VGE59_02595"
     CDS             complement(9839..11107)
                     /locus_tag="VGE59_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1074566.1"
                     /translation="MKKFFLLLKKEIRELLTPLMVLPILVTVVLFGAIGKIGKIESEK
                     AKSPQTITVIDQDNSSLSQQITTALGASNFKVVSAKLEDGATLAQTNNSVAYLLIPAD
                     LEDKVQSAQSPTLELHTFVRSLSASASTKQAIVKGAVAAINEAVSNQLLKSQTSLDPA
                     FAKQPIHVDEFVAVADREAHVSYEEVLGFVQSQTYFIPIILFLVIVFASQLIATSIAT
                     EKENKTLETLLSTPVSRQSIVAAKLVAAGLMALLFSAVYMIGFRSYMDGVTGGLSMDV
                     QGATAEALRSLGLKFGALEYALLGLSLFVGILAALSVALILGAFAEDVKSVQGVVTPL
                     MVLVMIPYFLVMLTDFSTLPQAAQWAVYAIPFAHPFLAIQNIALHHYPAVLYGILYEL
                     AFFLVAVYAASRIFSSDYIVTLKLNFSKKK"
     gene            complement(11104..11829)
                     /locus_tag="VGE59_02600"
     CDS             complement(11104..11829)
                     /locus_tag="VGE59_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002850633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074567.1"
                     /translation="MSQHILEVKNLSKHYGALAAVDDISFSISEREIFALIGPNGAGK
                     STTLRMVTTILSPTGGDVLVADHSVHKDPAKVREVISYLPEEAGAYKNLRGIDYLTFM
                     AGLYAKDAQEVKESLEFARGLTGLGNRLNDKISTYSKGMTRKLLLARTVMSKPKLAIL
                     DEPTSGLDIINAVEIRKSIKDLASHGMSVLLSSHNMLEIEFLSDRVGIMNKGKLLEVG
                     TPAELKKKYNATNLEEVFLAIVL"
     gene            12042..12377
                     /locus_tag="VGE59_02605"
     CDS             12042..12377
                     /locus_tag="VGE59_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY1074568.1"
                     /translation="MSKLSPHYQNARLISQFLDQEFKIGSFRFGLDPIMGLIPGIGDV
                     IPFLLGTYIVWVGMQAKLPQEAISRMVANLVIDLVIGAIPIAGDIGDFFFRANKRNME
                     ILEQFIVKE"
     gene            complement(12400..14058)
                     /locus_tag="VGE59_02610"
     CDS             complement(12400..14058)
                     /locus_tag="VGE59_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074569.1"
                     /translation="MSRKSSHSPSPHRGPLLFLFATWGTFFVALFGRMMLFRPDGLYM
                     GHNVVWSDWALHVALAQRFATLPVADWFVTHPLYAGGKLTYPFVVNMISGLLMRIGTP
                     LTWAFAIPSIVCVFAFIAGVYFLFFHLLRSRAQAVVGIFLFFLSAGMGFVPFLRDVFT
                     HHNWASLAYPPQDFSRLGQLSWETSNAITGMILPQRALLLGMTIGVWALAAIIKVFGR
                     KKEPSDGWLLMLAGAGIGLLPITHPHSLISIAIILLVIFVSYRTAWQRWLFLGFPAAL
                     IGIPLYLKFVGGGIENPHFFSLSLGWTATSFGGWFAQWFWQWGLTLPLAGISMWILWR
                     FGNGVSRSFFLGCFVLFLVGNIVQFQPIAWDNSKVFLWSYLGFCGLTAAFLVGLWRSH
                     GLVRPLAVALLITLTATGLLEMIRLQRVDQHTYLVTSQEDINLGEQIREETYTTDRFL
                     TAPVHNHFITLWAVRPILLGYTAWVYNYGFNYAQREADVKSIYTGTTDAISLLRDYGV
                     SYVVIGPAEKNAFTVNEDFFSSRFPVAFKNSQNTVYDTRKVLAH"
     gene            complement(14062..15477)
                     /locus_tag="VGE59_02615"
     CDS             complement(14062..15477)
                     /locus_tag="VGE59_02615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1074570.1"
                     /translation="MHLQFCGAAGEVTGSCSKLDVEHSSILIDCGLFQGDRFASEKNF
                     DPFPFKPEDIDAVILTHAHLDHCGRLPKLINQGFAGKIYATDATRDLTVLILLDAAHL
                     MGDEAERNGHPPLYTEEDVVHTMERFMPLPYDKEIKIATGVTATFHNAGHVLGSSFIE
                     VKGEGKSLIFSGDIGNHPVPLLEAPEDLPQADGIILESTYGDRTHGHYDEGIAALEEA
                     IKGTIRDKGVLLIPAFSLERTQELIRMIDEMITDHRIPAISAYLDGPLGIKISRVYEK
                     YEDLFNEETKELEAREPDHSVLTFKSLTITETAEQSKMINDVDPPKLIIAGSGMMQGG
                     RILHHLKRYLPLESTRVFVAGFQVQGSLGRRLLDGDRTVTIHGEQVVVNAKILGTQAF
                     SSHMDKTQLVEWLRTMKHLPKAVFLNHGDDEARAAFSKQLREELGIKKVLVPNYGDGY
                     TITDEEDDARLEYEEETTQAA"
     gene            15648..16919
                     /locus_tag="VGE59_02620"
     CDS             15648..16919
                     /locus_tag="VGE59_02620"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY1074571.1"
                     /translation="MDDKDKKEAERIEELKNMIDKGTSEKTETEEAEEEPYNTPPKSE
                     KDAKRASKEAKPSAMEDPEKRKKMIIVGSTVLLALLVATAVIAAVLSRPAEDNDVTTP
                     ISVATSSEPKATATPVPVTQASNLDGVVVANELATRHPLAIMIENSTLARPQTGLTDA
                     SVVYEAIVEGGITRFMAVYGHTLPAKAGPVRSARPVFLDFASEYTPRTAYYAHVGGSP
                     EAIDRIKKENVYDLDQGGIGTKAFQRFPKAGLATEHTMYTFPEKLLEIAKSRNYSTDA
                     TFKSWKFKNDAQIADRPESQSITIPFSSASYEVKYIYDKTSNTYKRYMAGVEHKDAVS
                     GKQIAPKNIIIHFANYATTDVKGRQDVDVVGTGTAKIFRDGKVIEATWSKSAPSARSI
                     YTDTATGQEVELNAGQIFVEVPKTGSVITVQ"
     gene            complement(17277..18017)
                     /locus_tag="VGE59_02625"
     CDS             complement(17277..18017)
                     /locus_tag="VGE59_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1074572.1"
                     /translation="MFKHMFDGVKEGNVIRYPANIGKKLLVGSVTFGLIPLQILMPAF
                     GAESSATPSVWKTELVLADETPELLTLEPQAPVIEKTASRYQEEQARLAEQLKREQAI
                     AAQQPKSKPKVVAPVTPKATVVADVPLEQKIALAQKAAATYGIPADILIAVWKVESGM
                     RWYFCGGSSVGARGPAQFMPGTWRGYGVDGNGDGVKDICHAEDAIPSAARYLAANGAN
                     RGELQRALLRYNNAQWYVDKVLALAGRS"
     gene            complement(18830..18985)
                     /locus_tag="VGE59_02630"
     CDS             complement(18830..18985)
                     /locus_tag="VGE59_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074573.1"
                     /translation="MNRKIGMHPLKNNGIPGGMNDAIPGTNLKNSSGILTIHARGGDS
                     GIPVPGA"
     gene            complement(19314..20111)
                     /locus_tag="VGE59_02635"
     CDS             complement(19314..20111)
                     /locus_tag="VGE59_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY1074574.1"
                     /translation="MEVTAELVVQQLGSPTAKGPNERLSQGIYRGNVAKSHKGRLRAL
                     VGREGTGKNAIGVLMQQATGVPRIGTGDLIRAVCKPVTEDDEILCCAVTPEEVESLKA
                     RKSGSRGNNAIVQKLLYARVLWWEDCQEGFILDGCPRDEYQPGWVSDLAVALDLGVDV
                     ILLEAPEEMLIDRVLHRLTCTRCKLDFNTKLKPPVVAGLCDVCGMELQQRDEDATADS
                     VRERWVDSDRVLSWLRLYYAQYSALYVVQAVGDPKDICRVTLEELLR"
BASE COUNT         5652 a         5246 c         4745 g         4890 t
ORIGIN      
        1 gcagcttgta cctcaccgat atattcactg ccagggagga gaatagcccc aacaacacgt
       61 tgtacgccta cggggtggat aggggacagg ccgagatcgt gggattccgc tggtttctga
      121 ccggttagca accggcaaca tcgttaacga ctgcggcttg cctttcgggg caggccgcgt
      181 ttctttttct tctggcaaaa ataacctata atacggatat gccgattcaa gcattacgtg
      241 gattccgaga tttttatccg gaagaccaag ccaccattac ctatcttcgc gaaaaaatcg
      301 cagcctcctg cgccaaattt gggtacgaag aattcgaagg ccccatgctt gaatcaatcg
      361 atctctacgc agccaagtcg agcgaagaaa ttgtaaatga gcaagccttt accttagaag
      421 atcgaggagg tgaccgcatt acacttcgcc ctgagctaac cccaaccttg gcacgcatgg
      481 tcgcagccaa gcagcaacaa ctcaccttcc cgctccgctg gtggtgcttt ggccgttttt
      541 ggcgctatga acgacctcag aaggggcgtg gacgcgaatt ttatcagtgg aacttagatc
      601 tactcggaca aaatactgct gacgcagacg ccgagattat cgaaatatca atcgacttct
      661 tgcagtcggt aggagtaacc agcgatgacg ttgtctttga gatcaacgac cgctcatttg
      721 ccgaacaact tttacgcgaa aataatatcg acaccgagaa gacttccgct gtttttcagc
      781 ttgtcgaccg attagccaaa ctgaacagcg aagaaaagtt agcctttggc ctagctcgtg
      841 gactgtccga gtcagacgtc accaagatta cggaactttt ttcagcggag agtacagctt
      901 gggagcaatc cgaacggatc caagctcttc tcactttgtt tgaaaataaa ggtttatcct
      961 cgtgggtacg accgaattta tcgattgtcc gaggatttac gtactacacc ggacttgtct
     1021 ttgaagtcac tgtcaaaaca ggccagagcc gcgccctcct cggcggaggc agatacagta
     1081 atttagtgtc cgatatgggc ggccaaccag ttgaaggcgt aggcatggga atgggggata
     1141 tgtctattgc cattttcctc gaagaaaaag gattgctgcc tgagtacaca acttctacca
     1201 aagcctgcgt gatcggactt ggcccggaca ccgcggcatt ttgccaagaa gttgccacca
     1261 ccttgcgcaa cgctactatc ccaacgattt tatacggaac accagagaac tttggcaaag
     1321 gcctcaagtt tgcaggaaat aaaaaaattc cctacgccct cttaattggg cccgacgagc
     1381 ttttgaataa aaccgtcact cttaagaacc ttgagacagg ggaacaagaa acaagctccc
     1441 tcgagacgat tctcgaaaaa ctcacctaat atttacctcc aaccagcaag gcaggggatt
     1501 gacaaaacgc cccgcctatg atatatattt tacatgtcga ttttttaagg gaggacgaca
     1561 tcatggctgc agcagttgca gagccgactc gagtagatgc cccgcctacg gcgggcgagc
     1621 tccggatgtt cgaattggaa gcccggcgag aagccttgga gagagaactc gaagatatca
     1681 atcgagaact catcgagctt cgacaggcgg ccgatctctg cgaaacgtgt ggcggaacta
     1741 agaaagtacg ggttcgagga ggactctacg gagaactcac cgagcgacca tgcgcctgcg
     1801 cagaaacgaa gaagtagcga cttcggtcgc attcccatca tgggacacac gagaacgccc
     1861 cggccattcg gccggggcat tcattttgtt cgaattttga ttgagacggt ggcgcctccc
     1921 aggcggcaaa gaagggggga ctctgcaaca cctgttgaat ggaagcacca ccctcccaac
     1981 caattacacc gatagtgtag catacgaccg aacgatgtca atattattat atgcctttac
     2041 ccttagcgtt aacacatcct ctaatgacgc gtttgactcc gtcccatttt tctattatca
     2101 gcccttgaca ccgaacgtaa tacgcatgct atggttagtt acagccgaac aaagagcgag
     2161 cagacaaaag gaggaccatg ctaccactta cgccgcgaca acgcgaaatc ctcgactaca
     2221 ttactgggtt cgtcgacagc aacgactaca gcccctcgta ccgtgagata gccgaacatt
     2281 ttaatctctc cagcacagca accattgccg aacatatcga aacgctcaag acaaaaggat
     2341 atctacgtga cgacctgtct ggcgtccgtt cgctccaaat ccaagcgccc gagccacgtc
     2401 aagaagactt gctccacgaa atggcaatcc cgcttttagg ttccattgct gccggattac
     2461 ccatcgaagc gattcgcacc aacgaaacaa tcgatattcc tcgcgacatg gcaggcaaga
     2521 aagtcttcgc ccttaaagtt cgtggagact ctatgatcga agatggaatc atggaagggg
     2581 actatgtcat tatcgaggct tgcgataacc ctcgaaatgg tgatatcgta gttgcgttgc
     2641 tcgatcgaga caacgtaacc ctaaaacggt tctaccgtga gaaagatcac gtccgtctcc
     2701 agccagccaa caaaaaatat aagccgattc gcgtgaaaaa ggttgttatc caaggtcgag
     2761 tgaaaggcgt gattcgcaaa tttgattagc cttcgcgact cctgcacttt ctcggttcat
     2821 atcttttgct aatttagccc cttccgaggg gctaaatgtt gtgatttcga catttgctcc
     2881 ccctcttgaa aagtggtata taagattgta taataactta gtaaaacagt agtggaggcc
     2941 tatgcgtaaa gagtacaaac gtaccctcat caaattcagt aactatagcc tgtgcgtaac
     3001 attacctaag gctatgctca aaaagcttga gctcgataaa ggaagcgaag ttctgatcac
     3061 cttaaaacgc ggtaaactta tcattgagcc agccacggct aatggcgaag ctgcatcaac
     3121 cgaccaggat aatgataact gggaacagat gcaagaaatc aacgaagacg aagaataaac
     3181 gccttcaccc ttgctcgtca ctgagaatat gtgcgtgacg agcaagccca ttgtgggata
     3241 taatgctagt agaatgccac attttttcgt aagcttacga caatgaagaa acatatcgat
     3301 atcgttcgaa aaacaaagcg gcagactatg gatgaagcgg ttttaaagcc aaccaccgcc
     3361 actgagacac ccgagccagc tctcaaacca ggagaagtca aaacttctcc cgtgattgtt
     3421 ggattactcg tgattgtcat tggcattatg gttggttttg tgttttattt atttaccatt
     3481 cagccctaaa cgggcaaata aaaaaacttc ttgccagcag gcatcccgag agggacttcc
     3541 ttggttcgaa gtccccaggt tgcgtagcaa tttgggtgaa aggagatttt catggaatac
     3601 caacatcagc gcgtggcggt tttcgtcgat gtgcaaaatt tatatcattc agccaagaat
     3661 ctatacagtg ctaacgttaa ctttagccaa attttgcaag acgcggttgg aaaccgccag
     3721 ttagttcggg caattgctta cgtggtccgc gccgaaggac caaaagaaca agcctttttt
     3781 gacgcactag agaaagccgg attcgaactg aaagtcaaag acattcagac ctttgctggt
     3841 ggcgctaaga aagccgactg ggacgtgggt atggcaatcg acgcgttaac catcgccaag
     3901 catgtcgatg tggttatttt aatgaccggt gacggcgact tcaccccgct catttcgtac
     3961 ctccaaaaca gcgaaggtaa tttagttgaa gtggccgcct ttggtcgcag ctcttcatct
     4021 cgcttagttg aatgggctga taagttcctt gatttcgaga ataacccaga acgttacctc
     4081 ttaggctacc gatctccgga aaaagccgag aaaccaacct cagccgaaac gactgaagaa
     4141 gcaatcgggt aaggccgcca tgcagcgggg ctggcttatc ctaggcgttc tgctcctttt
     4201 gggggccggc tattttattt tctctcccaa aaaggactat tgcgaagtga ccacacttac
     4261 cacgcccaac gggacaaata ttggggtcga aattgccaat aatgggctcg aacggacccg
     4321 tggcctcagt aaccgtgact cacttccaat taaccgagga atgctctttt tatttccgac
     4381 ttctgacatt tacagcttct ggatgaaaga taccaagatt cctctcgacc tcctctggat
     4441 tcaggacacg acgattgtcg atatcaccac tcttccggcc gaaatccctg gggaaaccat
     4501 cccacagtat cagccaaaag cggaagccaa ccgagtgtta gagctcaata gcggagaagc
     4561 agccaagcac gggctcaaaa tcggatctca cattataatc gacaaaacct gcttgccacc
     4621 agcaagcatc taaaagatgt cactggcgta cctttttggc cttattatcc tcatggggct
     4681 cttaagcaac tggcttagca aggccttagc aaccatggcg ccctattcca tcttccgttt
     4741 agctcttctg ccggggatta ttttgcacga atctgcccat gtggcgggct gttggcttac
     4801 cggcgcacgt gtccatcgag tacatttttg gacacagagc gggggagagg tgatccacac
     4861 gcaaccgaag attcctgtga taggccctcc catcatttcg tttgcaccat ttgtagttgg
     4921 catgtacgcc ctgtggcatc ttggaatcta cctgaccgtg ccaccgttag aaatttcgtg
     4981 gcaacgagga ctggccgtct ttttaatgat gagcattggc gccaccctta ttccaagtaa
     5041 acaagatttt aaaaatagct tcggcagcct ggtagttata gccatccttg ccagcgctgc
     5101 gttatatttc ttcccttcac tctggcaccc actcaacacg tatgcccaac cccttttaaa
     5161 gccctttatt ctcgttaccg caattattgc aaccttaact ttcctcgcga atctcactat
     5221 gtggcgccgc tagccagttg acaagcaggc ataataagca atatactaag gcggtcaact
     5281 tctgcacgcg tagtgtgaaa ggatccgtcc tagcatgtac ctcttcttga cgcagttaac
     5341 ctatgtagag gagtacctga tgaaccaacg gccgtcgaat gccgcctata ctaagagccg
     5401 aatcaggttg ggtaactcgt ctcgacaagt cccgcccatt ggcgggtggt acaaatccag
     5461 gcgtcgaaga cgccgcgtct ccagcttcat cgcctagacc cctccgggtc ttcccaccgc
     5521 cctggccaca ccccattcac cccgtggccg gggcttctgt ttttatttaa agaaaaaact
     5581 gttaccgctt ttccttttta cacaagacgg aactcacttt tatgttatag tagtttgcat
     5641 gcagtcactc ttttctgacc cttcgaaaat catttatacc gtcaaagaat ttaatgaact
     5701 cgttaacatt gtcctcaccg agaaggtagg ggaagtgtcc attcaaggcg aaattaccgg
     5761 gtaccaaatc cgacaaggca aatggatttc ctttgacctt aaagactccg aaagcgtgat
     5821 taactgcttt gccacgacct accaaattga tatgcctttg tctgatggta tggaagtagt
     5881 ggtcacaggc acgccacgtt tgtatgtccc atatggcaaa tacagcttta atgttcgaaa
     5941 tgtacaactc aaaggtgagg gagcactcaa aaaagccttc gaggatttaa aaaagaagct
     6001 cacaaccgaa gggctctttg atcccatcca caagagatcg ctcccacgct ttcccgagaa
     6061 aattgggatc atcacaagtg gcgaaggtgc cgccgtgaac gacattctta aagtgataaa
     6121 cggacgatgg ggagggctgg agatttattt agcacccgta ttagtccagg ggaaaaatgc
     6181 gcctgatgag ctcgttggtg ccattcaata ctttaatcag cgctttcccg ttgatgtggt
     6241 tatttttggg cgaggcggag gatctctcga agatttacag gcttttaaca gcgaacgtgt
     6301 tgcccgcgca attttcgctt ctcgaattcc gattgtggcg ggtattgggc acgagcacaa
     6361 cacctctatt gctgatctcg ttgccgatgt tcgcgctgcc acccccagca acgccgccga
     6421 aatttgcgtg ccggaccgag ctgcaatcgc tcgagaagta gaatttcttt ctcataaggc
     6481 agaacggctg gtacagcaac gcattatgag ccatcgccat caccttaata atcaaacgca
     6541 aaccttagag ctctttgtga gtacacgctt tgctcgcatc cgatctttaa ttacccgggt
     6601 accgcaactg cttcattcgt atcaagagaa gacgcgcctt atacaccaaa aaattgccat
     6661 gagccacact catctcagcc agagaatgaa cgaccgattc gagacttctc ttcgtcagct
     6721 caaagaaaaa gaaaaactcc tggcagcact caatccaacc gccatcctga aacggggcta
     6781 cagtattacg tacgccacgg gcaccggtac cccaattacc cgtttggcag acattcccgc
     6841 cgatggtaga atcagaacgg ccttaagtga cggaattatt gaatcccata tatcagaagc
     6901 gggaagagag attaaaaaat cgccgccagc caaaaccgtt aaaatcacct tagaaaaagc
     6961 gcaagaaact ttattttaat ttaaaggaca ccctatgcaa aaagaacaaa agtttgaaga
     7021 gattgtaaca gaactcgaaa caatcgtttc ggagcttgat agctctatcg gcctcgaaga
     7081 atccctcaaa aaattcgaac gtggaatgga attagccaag cacgccgagg aacgtttaaa
     7141 aactatcgaa aataaattcc aaaagattca acagcagttt gttcccgaag ccgaggtaat
     7201 tgagcaaaca gaggaataaa cttatgcgcc ttttccggta gtaacaaaca actaagaaaa
     7261 aacactctct ccccgatgga gagagtgttt tttctcctgc ctgtattgcc cgaatagcca
     7321 gtatatgtta ttattggtac gttcgctccg tgaccatagg agatagatcc atgaatcgtg
     7381 agtggcagaa gatttgtgta atctgcctca ccgtgccgga tattggcact ccacaagccc
     7441 ccacagaagg ggatgcggta attacggtac aaacccagat cgctgcgcat atgatgaaaa
     7501 cggttggcgt ggaactgggg acgtatacac ggctagccgc tgcaattggt ccggacaccc
     7561 ggccgatgct gctccctaaa gcaatgtatg aaggccccac acctgaaagg ccaaactcag
     7621 gtccacctcc atcgggacga tgggcccacc tacaaggcag ataccccatc ctaaggtctc
     7681 gcccagagtc tccgtgcctc ttgaaccccc ctccggaaaa ctcgatcccg ctccaaatta
     7741 tgaccgcgtt tctcaacgga gtgggacagt ctgactgtcc catctacgag gataacgtct
     7801 tcgccttgga gtgccgggac aatgcgcttg gaaagtcttt cggagccatg cttgagccag
     7861 ccttacgcca gagcgtaggg gacaggatca tgcgtcagtt gcggaagcac tatacccctg
     7921 gagtcagtgg actcatgggc gatccttcgt tgatctatca ccttggtcaa caacggatca
     7981 agctctggac gtacattaag tatgtcgccg agctgatcga cgaccgacaa gtggacggat
     8041 ttgatcccgg aagtaacttt gccattctgc tcgtactgca acatccgttt gcaatcgagc
     8101 agatctggca taccctcgtc ggtgaacggc aagtgccccc agggggcgca atgctgaaca
     8161 cctatcatcg caaggtggta gaaggtgagg actacatgta cctggcgaaa gccgctcgtg
     8221 tctatccgcc cagcattggc cgtagaccag tcattgtggg gttccccccg ccccctctcc
     8281 ttcgctagaa ggcaagatcg ccccgggcgc cactctatgg cgccgggggc ttttcttttt
     8341 tctggtataa tttgagcatg cacaagattt tttcgcgcat tgtcattgga ctgctcattc
     8401 tttttggtct ctatataatg ctcggaaact tagattctct gctcgattat caggatttta
     8461 agatgatcat tgtctacggg ctagatctag ccaaagatgc aatccatatt tacctcggat
     8521 ttttagcttg gctgatttca gttctgtttt ttcatcgctc acctcgaaca ccacaggttt
     8581 tggtgcttgg ctttgtattt tctctcctta tggagttagc cgacatacgg ggagattgga
     8641 ttcaataccg ttctcctgac tggatgggca gtcttcacga catcgtccat accaatatga
     8701 tcccgacttt actcttcatc ttttttcgaa cctataaaga ggaagaaata aaatgaatca
     8761 tcgagtggta gcctctgctg taatctataa agataatcat atccttatcg caaaacgagc
     8821 agcaacaaaa aaagtttttc caaacgcatg ggaaatacca ggcggaaaag tagaggtggg
     8881 ggagagtcct cagcaagcac ttaaaagaga agtaaaagag gaactgggaa tcgatatcga
     8941 agttatgcaa ccactctgcg tatacagttc ccaaattggc aatgaatggt ggaccgagat
     9001 agactttgta gctgtattac ttttgccatc tcaaactatt actcttaata cccacgacca
     9061 ttctgactac gcatggatta atcagtcaca aattgatgaa tattttgatg agacagataa
     9121 caatggaagg gctgttcgcc aagcgtttca agagttatga cacttataga tgaaatcctt
     9181 acagacctca aatcacttga gtctgaaaag aatcgagcag gtatggcgcg atttggcatc
     9241 caagttgaac acgcctacgg tgtttcagtt gccaatattc gcagactggc caaaccctat
     9301 aggcataacc atgaactagc gcttgaatta tggaaaaccg gtattcatga agcccgcatt
     9361 ttggccgcaa tcatcgacga cccaaagcaa gtaaccccgg agcaaatgga caggtgggtt
     9421 cagaatttta actcctggga tctttgcgat caaacctgca gcctcttcga caaaactcct
     9481 catgccgtgg caaaaataaa agagtggacg acacgtgaaa aagaatttga aaagcgagct
     9541 ggctttgccc tcctggctga attagcatgg cacagcaaga cagaacccga tcaaacattc
     9601 ttggatttac ttccactcat agaacgagaa gctcatgacg atcgaaatta cgtcaagaaa
     9661 gctgctaact gggcattacg tgaaatcggc aaacgtagca tgagacttca tgccgaagcc
     9721 atcaaattag cacagcggct ccaaactcac gactcaaaat cagcccgatg gatcgcgagc
     9781 gacgctctac gagaactgac ggccgaaaaa acgctctctc gcatcaacaa gtaaaaagct
     9841 attttttctt tgaaaagtta agcttgagcg tgactatgta gtcacttgag aaaatacggc
     9901 tggcagcgta cacggcgacc aagaagaatg ccaattcata gagaatccca tagagtactg
     9961 ccggataatg atgtaaggcg atattttgaa ttgccaagaa gggatgagca aatggaattg
    10021 cgtagaccgc ccattgtgcc gcctgaggaa gagtcgaaaa atcagttagc ataaccaaga
    10081 agtaggggat cataaccaaa accatcagtg gcgtaactac cccttgaaca ctttttacgt
    10141 cttcggcaaa cgcacctaaa ataagtgcca ctgataatgc cgccaaaata ccaacaaaaa
    10201 gggataaccc taagagggca tattctaggg cgccgaactt tagtcccaat gaacgaagag
    10261 cttcggccgt tgctccttgt acgtccatag acagaccacc tgttacaccg tccatataag
    10321 aacgaaagcc aatcatatag accgcagaga acagtaacgc catgagacca gccgcaacca
    10381 gctttgcagc cacaatcgac tgacggctaa ccggcgtact gagtagtgtt tccaaggttt
    10441 tattttcttt ttctgtcgca atcgaagtag cgatcagctg agacgcaaag acaataacca
    10501 gaaagaggat aatagggatg aagtatgttt gcgactgcac aaaccctaaa acttcttcat
    10561 agcttacatg agcctctcga tctgcaaccg caacaaattc atcaacgtga atcggctgct
    10621 tggcaaacgc tggatcaagc gaagtctggc tcttgagcaa ttggttactt actgcttcgt
    10681 taatcgccgc cacggcacct ttcacaatcg cttgcttagt cgaggcagag gcggaaagac
    10741 tgcgtacaaa cgtatgtagc tccaaggttg gagactgcgc tgattgtact ttatcttcga
    10801 ggtcagcagg aatcagtaaa taagcgacac tgttattcgt ttgggcgaga gtcgcaccat
    10861 cttccagctt ggcggaaacg actttaaaat tactcgcccc aagcgctgtc gtaatctgct
    10921 gtgacagaga cgagttatct tgatcaatca cggtaatggt ctgtggggat tttgcttttt
    10981 cggactcgat cttaccaatt ttgccaattg caccaaacaa aacaactgtc accaaaatcg
    11041 gtaataccat taaaggagtc aatagttcgc ggatttcttt tttgagtaga aggaagaatt
    11101 ttttcataag actatagcga ggaagacctc ttccagattt gtggcattat actttttctt
    11161 tagttcagca ggagtaccaa cttcaagcag tttaccttta ttcataattc ccaccctgtc
    11221 agataaaaat tcaatctcaa gcatattatg gctcgacaac aatacggaca taccatgcga
    11281 tgctaaatct ttaatagatt tacgaatttc gacagcatta ataatgtcaa ggccgctcgt
    11341 tggttcgtcg agaatcgcta acttcggctt tgacataacg gtccgtgcga gtaataactt
    11401 acgggtcata cctttgctgt aggtacttat tttatcgttt aaacggttcc ccaaaccagt
    11461 taatccccga gcaaattcca ggctctcttt aacctcttga gcatctttgg catagagacc
    11521 cgccataaag gtcaggtaat caatgccacg taagttttta taagctccgg cttcttccgg
    11581 taaatagcta ataacttcac gcactttagc tggatcttta tgcacgctat ggtctgcgac
    11641 gagcacgtct cccccagtag gagaaagaat agttgtgacc atccgaagcg tagttgattt
    11701 gcccgccccg tttggaccga tcaatgcaaa aatttcacgt tcggaaatcg aaaaagaaat
    11761 atcatcaacg gcagcaagag caccatagtg tttcgaaaga tttttcactt caaggatatg
    11821 ctgtgacata ccagaactgt accaatattt tctgagccaa tccagagcgc atataacgaa
    11881 gcttttaata aataaccaat ggaacgttgt atttttgact acaatttagc aaaaagtaaa
    11941 tagaaatcgg cgtattactt tggcactata tccctaccac ggacattcgc ttctccactt
    12001 ttaggagaac atcatatctg tccgtggatt tattgtcaca catgtctaaa ttatctccac
    12061 actatcagaa tgcccggctc attagccaat tcttagatca agaattcaaa atcggctcgt
    12121 ttcgttttgg acttgatcct attatgggac tcatacccgg cattggcgat gtaattcctt
    12181 tccttcttgg cacttatatt gtctgggtag gaatgcaagc taagttacca caagaagcaa
    12241 taagccggat ggtcgccaat ttagttatcg atctggtaat tggcgcaatt cccattgctg
    12301 gcgatattgg tgattttttc ttccgcgcca ataaacgcaa catggaaatt cttgagcaat
    12361 ttatcgtcaa agagtagagt tagcccctct tcaaaacaat taatgagcca gaaccttacg
    12421 ggtgtcgtaa accgtgttct ggctattttt aaaggcgacg gggaagcgac tggagaaaaa
    12481 atcttcgtta accgtgaatg catttttctc ggctggccca attacaacat aactcacgcc
    12541 ataatcacgt aaaagcgaaa tcgcatctgt cgtgccggta tatatagact ttacgtcggc
    12601 ttcgcgctga gcgtagttaa agccataatt ataaacccac gccgtgtatc ccaagagaat
    12661 tggacggacg gcccacaagg ttatgaagtg gttatggacg ggcgcggtta agaatcgatc
    12721 ggttgtgtac gtttcttctc gaatctgttc acccaagttt atatcttcct gggaggtcac
    12781 taaataagta tgttgatcca cgcgttgtaa ccggatcatc tctaatagtc ctgtcgcagt
    12841 cagcgttatc aggagtgcga cggccaaggg acgcaccagg ccatgacttc gccacaagcc
    12901 tacaagaaac gcggccgtta aaccgcaaaa ccctaaatag gaccacagaa aaaccttgga
    12961 gttatcccaa gcaatcggct gaaattgaac aatattcccc acaagaaata aaacaaaaca
    13021 accaagaaaa aagctacggc tgacaccatt tccaaagcgc cataaaatcc acatacttat
    13081 acccgccaaa ggaagcgtca gcccccactg ccaaaaccat tgggcaaacc agccgccaaa
    13141 actcgttgcc gtccacccta aagagagaga aaagaagtga gggttctcga tgccaccccc
    13201 aacaaacttt aagtaaagcg gaataccaat taacgcagca ggaaagccaa ggaataacca
    13261 tcgctgccaa gccgttcgat aacttacaaa aattacgagc agaataatgg caattgaaat
    13321 caggctatgg ggatgcgtaa taggcagaag cccaatacca gcgcctgcaa gcattaaaag
    13381 ccacccgtcc gagggttctt ttttgcgccc aaacacttta atgattgccg ccaaagccca
    13441 gacgccaata gtcatcccaa gtaagagtgc acgttggggc aagatcatcc ctgtaatagc
    13501 gttgctcgtc tcccacgaaa gttgcccaag acgactgaaa tcttggggcg ggtacgcaag
    13561 cgaagcccag ttatgatggg tgaatacatc gcgcaggaat ggcacaaatc ccatcccggc
    13621 actcagaaag aagagaaaaa tccctacgac ggcctgggca cgagaacgaa gtaagtgaaa
    13681 gaataaaaaa tacactccgg caataaacgc aaatacacac acaattgatg ggattgcaaa
    13741 agcccatgtc agtggtgtcc ctatgcgcat cagaagacca ctgatcatat tgacgacaaa
    13801 cggatatgtc agctttccac ccgcgtagag gggatgcgtt acaaaccaat ctgccacagg
    13861 taacgtagca aaccgttgcg cgagtgctac gtgtaacgcc caatcactcc acaccacatt
    13921 atgtcccata tagagcccat caggccgaaa gagcatcatt cggccaaaga gcgctacgaa
    13981 gaaagtgccc caggttgcaa acaagaagag taggggtcct cgatggggag aaggggagtg
    14041 agatgattta cgcgacatac cctatgcggc ttgagtcgtc tcttcctcgt attcgagacg
    14101 agcatcatct tcttcatcag taatagtgta cccatcaccg tagttaggaa cgagcacttt
    14161 tttgattccg agttcttcac ggagctgttt cgaaaaagca gcacgagctt cgtcatcacc
    14221 gtgattaagg aatacagctt tgggtaaatg cttcatcgta cgaagccatt ccacaagctg
    14281 cgtcttatcc atatgtgacg agaaagcttg cgtaccgaga attttggcat tcaccaccac
    14341 ttgttcgcca tgaatcgtaa ccgtacgatc accatctaac aaacgacgcc ccagcgaacc
    14401 ttgaacctgg aatcccgcca caaatactcg ggtactttcc aaaggaagat accgcttgag
    14461 atgatggagg atacgtccac cttgcatcat gcccgaccca gcgataataa gcttgggcgg
    14521 atcgacgtca ttaatcattt tcgactgctc ggcggtttcc gtaatcgtca ggctcttaaa
    14581 ggtcaaaaca gaatggtcgg gttcacgcgc ttcgagttct ttggtctctt cattaaagag
    14641 atcttcatac ttttcataga cgcggctaat tttaatcccc agaggaccat cgagatacgc
    14701 cgagatagcc ggaatcctgt ggtcggtaat catttcgtcg atcatacgga taagttcttg
    14761 cgtacgctcg agcgaaaaag caggaatgag gagcacgcct ttgtctcgga tagtgccttt
    14821 gatggcctct tcgagtgcag caatcccttc atcatagtgg ccatgggtac gatccccgta
    14881 agttgattct aaaataatac catcagcctg tggcaaatct tccggtgctt ctaggagggg
    14941 caccggatgg tttccaatat cacccgagaa aatgagtgat ttgccttcac ccttgacctc
    15001 gatgaaactc gagcctaaga cgtggccggc attatgaaac gtggccgtta cgcccgtggc
    15061 aattttaatc tctttgtcat agggcaaggg catgaaccgc tccatcgtat gaacaacgtc
    15121 ttcttcggtg tagagcggag gatgaccatt acgctcagcc tcatcgccca taaggtgagc
    15181 cgcgtcgagt aatatcaata cggttaagtc acgagttgca tcagtagcgt aaatcttgcc
    15241 tgcgaaacct tgatttatca gtttgggtaa acgcccacag tggtctaaat gagcgtgcgt
    15301 taaaataact gcatcaatat cttcgggttt aaaagggaaa ggatcgaagt ttttttcaga
    15361 cgcaaaacgg tctccttgga agaggccgca atcaataaga atggaagaat gttcaacatc
    15421 aagcttactg cacgatccgg ttacttcacc tgccgcgcca cagaattgta aatgcataac
    15481 cgttatctcc tttacttcaa acccctccag taataagggg ttttcacaat ctttctctca
    15541 gcctagcata tttttctctg tcctacacgt ttgccactgg agagaaattc tggcatgctg
    15601 agactatctc aagaggcgtt ttggcgccgt acgaaggagg ctattgcatg gacgataaag
    15661 ataaaaaaga agccgagcga attgaagagc tcaagaatat gattgataag ggaacctcag
    15721 agaagactga aactgaagag gctgaggaag aaccgtataa caccccgccc aaatccgaaa
    15781 aagacgcaaa acgtgcctca aaagaagcga aaccttctgc tatggaagac cccgaaaaac
    15841 gcaagaagat gattattgtg ggcagcaccg tgctactcgc cttactcgta gcaacagccg
    15901 tgattgccgc tgttctgtct cgtcctgctg aagacaacga tgtgaccacc cccatttctg
    15961 ttgccacctc tagcgaacca aaagcgacgg caacgcctgt accagtaaca caagcctcca
    16021 accttgacgg agtcgtggtt gccaacgaac tcgccacacg tcatcctttg gcaatcatga
    16081 tcgaaaacag caccttagcc cgtccacaaa ccgggttaac cgatgcaagt gtagtctacg
    16141 aagccattgt tgaaggcggc attacccgct ttatggctgt ctatggccac acgttacccg
    16201 ccaaagctgg ccctgtacgt tccgctcgtc ctgtattctt agactttgcc tcggaataca
    16261 ctcctcgtac tgcttattac gcccacgtcg gtggatctcc cgaggctatc gaccgaatta
    16321 aaaaagaaaa tgtttacgac ttagaccaag gtgggattgg cacaaaagct ttccagcgct
    16381 tccccaaagc cgggctcgcc actgagcaca ctatgtatac cttccccgaa aagctgctcg
    16441 agattgccaa atcacgcaac tatagcaccg acgcaacatt caaatcctgg aagtttaaaa
    16501 acgatgccca aatcgccgat aggcctgagt ctcaaagtat tacgattccc tttagctccg
    16561 catcgtacga agtaaagtac atctacgaca aaacgagcaa tacctataaa cgttatatgg
    16621 cgggcgtaga acacaaagat gccgtttctg gcaagcaaat tgcacccaag aatattatta
    16681 ttcacttcgc caactacgcc acgactgatg tcaaagggcg ccaggacgtt gatgtcgtcg
    16741 gtaccggaac cgccaagatt ttccgcgatg gtaaagttat cgaagccaca tggagcaaga
    16801 gcgccccatc tgcccgatcg atttataccg acacagccac tggccaagaa gtcgaactca
    16861 acgccggaca aatctttgtc gaagtcccca agaccggcag cgtaataaca gttcaataaa
    16921 aaacttctct aaatgcgaag agcgcctcac tgaggcgctt tttcgaaaaa gagaaatggg
    16981 gtgagcttgg cccgaaggcg gaaaacctta gccaacgggc caagctcggt tggggaggcg
    17041 aggcgcagaa ccacctcacg taatggggac actgtagtac atctttacgg cgggagccga
    17101 aggtcggtac tcataacctc gaatttcgcg tgtataacgc gactaggtca tcatcggagc
    17161 ttcctccata ccctgagcgt agggttatag tgagtatatc aaagtatttt agccatgcaa
    17221 gcacaacaaa agggagcctc atatgaggct cccttttgcg tttttgattg tacgtattat
    17281 gatcgacccg ctaaggccaa tactttatct acgtaccact gcgcattgtt gtaacgcagt
    17341 aaggctcgct gtaactcacc acggtttgca ccgttggcag ctaagtaacg agcggccgag
    17401 ggaatagcat cttcggcatg acaaatgtct ttgacaccgt caccgttacc gtcgacacca
    17461 tacccacgcc atgtgcctgg catgaactgc gccggacccc gtgcgcccac tgaagagccg
    17521 ccacagaagt accaacgcat accagactca actttccaga ccgcaatcag gatatcggct
    17581 ggaatcccat aggttgccgc cgctttttga gcaagggcaa tcttttgttc taacgggaca
    17641 tcagcaacta ccgtcgcctt cggagtaact ggtgctacaa ccttaggctt cgatttaggc
    17701 tgctgcgcag cgatcgcttg ttctcgtttg agctgttcgg ctaaacgagc ttgttcttcc
    17761 tgataccgcg atgctgtttt ttcgatcact ggagcctggg gctcaagtgt caggagctcg
    17821 ggtgtttcat cggccaacac gagctcagtt ttccagacag atggcgtagc cgaagattcg
    17881 gcgccaaatg ctggcatcaa gatctggagg gggataaggc caaaagtgac cgaacccact
    17941 aacagtttct tgccgatatt cgccgggtag cgaataacgt tcccctcctt aaccccatca
    18001 aacatgtgtt tgaacatggt taaggaatca catcctctct ggctcctcgg gtggtaaatc
    18061 cctggcatac gccacggcca cataagggca aatattgttg tgatggatta ttaatcgacc
    18121 actccggcac ccccgttgag acaatgtctc gtaggtttaa atgcggagct gccacccaat
    18181 gactttcgtc gctagagtca cagctcatca cttttttgcg gcaagtagag tataggccac
    18241 atcataccaa atatagcgta tactgtcaat cgttccgtat gtaaaattgg tcttttcgag
    18301 gcgaattttt cgcaagttct tataacgaag aagcccccgt gacgcgcatg ctgcgcgccc
    18361 gggggcttgg taaagtagga gccggattgg cccgcctagg gcggcgaccg tgcagacaac
    18421 ccggtggtca tccgctccgc agtcccgcgc gacgttgccg tccgccgaac tcctcactct
    18481 accgaggaat cactctctat ggggccaagc cgcccggctg tgccgccgcc atggtaaggt
    18541 ttaccacgcg gccatgggcc ggggctcgca ggggctccta gagcagtgaa aggtagccac
    18601 acccaccagt tgcccggtgg ggctatcgtt gccagaggta tcggatcatc ttctttaaac
    18661 tccggtgtga atgcctgccg ggaatctcac ccggccgatt tcacaagatc ccggacggga
    18721 tcgagtcacg tgccttgtgg cgagtcatct cgtcaaatct ccagagccgc ggcgcctagt
    18781 gccgctacga tcgccgaatg ccggcgccgc ggctctcagg cccacaggat caggcgccgg
    18841 ggaccgggat gccggagtcg ccgccgcgcg cgtggatggt caggatgccg ctggagttct
    18901 tcaggttggt gccggggatc gcgtcgttca tgccgccggg aataccattg ttcttcaggg
    18961 gatgcattcc gatcttccga ttcatcgacc ttcctccgta gagtgtgatg ttgaccccga
    19021 ggtggggtcg cagtgtgagg gctccgtgga ggattcaact ctatttctag aattgatcct
    19081 cccgacggag tacgcgcttt gcaagttgct acgttggcta tgagagccgt cgtgttgctt
    19141 tgctcagcgt tacaagcaaa ataatagcat ataacaaata ctttgtcaat accccctaaa
    19201 agggtaaaga gttaagaaat atttataaaa acaaaagtat cataataaaa agcagtaccc
    19261 ccgaccttac ggccgggggc aagtgagtgg atatggatga ggcgagcaga agactaacga
    19321 agcaactcct cgagggttac ccgacagatg tccttcggat ctccaactgc ctgtaccacg
    19381 tagagagccg agtactgagc gtaatagagc cgaagccaac tcagaactct gtctgagtca
    19441 acccaacgct ccctcacaga gtcagccgtg gcatcttcat cccgctgttg gagctccatt
    19501 ccgcacacat cacagagacc agccaccacg ggcggcttca actttgtgtt gaaatcaagc
    19561 ttgcagcggg tgcaggtcag ccggtgaagc acccgatcga tcaacatctc ttcaggcgcc
    19621 tcgagcagta tgacgtcaac ccccaagtcg agagcgaccg caaggtcgct cacccagcct
    19681 ggttgatact catcccgcgg acaaccatcc aggatgaatc cttcctggca gtcttcccac
    19741 cagaggacac gtgcgtagag cagcttttgc acaatggcat tgttaccgcg cgagcccgac
    19801 ttccgggctt tgagcgattc cacctcttca ggcgtcaccg cacagcagag gatttcgtca
    19861 tcctccgtta cgggcttgca aacagctcga atcagatcgc ccgtaccaat ccgaggtaca
    19921 ccagtcgctt gttgcatcaa tacgccgatg gcgttcttcc ccgtcccttc acgccctaca
    19981 agagcacgaa gtcgaccttt gtgggacttc gcgacattcc ctcggtagat accttgggac
    20041 aagcgctcgt taggcccctt tgcagtcgga gagcccagtt gttgaaccac caattccgca
    20101 gtcacctcca tgctacttcg cccccatccg aaaagcacaa aagaaaaaac agcgcgtgca
    20161 gtacgctgat ggggaaatta tatggtattt tcgtacgtcg cgtcaagcct ttgccacttt
    20221 tttagaaact tttttgcatt aattaaatat taatccctcc ttgtcccgtg cgggggtggg
    20281 agttgttaga gggtagggga ccgcttcggg agagtaggcc ccctaccctc taaccccggc
    20341 cggaggccag tctcataatt aagatttcag agcccgaaca taagccgaat ttatgatact
    20401 atgcttatat gaatctcttt tccgaggcag aagaaaccat cctagatcag gtcgcaccac
    20461 tggcggagcg catgcgacca aagactctcg atgactttgt gggccaagaa catatcttag
    20521 gcaaagatcg gcc
//
LOCUS       DASWYB010000045         7306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_796477, whole genome shotgun sequence.
ACCESSION   DASWYB010000045 DASWYB010000000
VERSION     DASWYB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796477"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..590
                     /locus_tag="VGE59_02640"
     CDS             267..590
                     /locus_tag="VGE59_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074575.1"
                     /translation="MNMYDFTFFAVGVASVLVSITLIALFIWGIMVMKRLNRLFDTLE
                     AISKSGLETTQAIHGFAEKTTEQLSMAIKTMVSIQGAKEIVGAIGEQFKKNKNEKESH
                     HGTTK"
     gene            574..960
                     /locus_tag="VGE59_02645"
     CDS             574..960
                     /locus_tag="VGE59_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY1074576.1"
                     /translation="MEQQNNKRGSMMGAALLGAALGAAAGLLFAPKSGKETRQDLTDL
                     MGRMSNEIGDRTSKLREVTKSAYNEIVDVVVKQYQEAKEITTEQAAKLREELRQGYER
                     VKAVADEHKLPEGTSVEESPKQKSKK"
     gene            complement(1024..1857)
                     /locus_tag="VGE59_02650"
     CDS             complement(1024..1857)
                     /locus_tag="VGE59_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1074577.1"
                     /translation="MLALELLAPHNPLVIAPLFLSYQEQRELVLQWQRGRNRQILERL
                     IASFICLVRAEAGKVEPHCNSLSYEDLVGHGIDGLIHGFRKYKKSYRTRVSTYVTPWV
                     RQAMKRAKENGDSVIRVPVHIHEARAKLATLWNKTYQSEHRSPTWEEMLSATGLNSGQ
                     LEAALLGVGECVYSLDEPVTEDQSPDFFVDVVPCDEPHPFDTVGRSVTVDVVQYAWGR
                     LPKKKWRDVIRLRYGLGCPPHTQLAAAEVLRMTKQAVSQAEKRALTFMRPILYRHLAM
                     G"
     gene            1980..2561
                     /gene="lepB"
                     /locus_tag="VGE59_02655"
     CDS             1980..2561
                     /gene="lepB"
                     /locus_tag="VGE59_02655"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY1074578.1"
                     /translation="MFSFIQSVVITVLLAFLLRFFVLQPFIVEGSSMEPNFHDQQYII
                     IDKLSYRMREPHRGEVIVFHPPVAITQNYIKRIIGLPGETVLVKEGEVFVNGKKVDEP
                     YLGNENHHTLPISTQSPVTLGDDEYFVMGDNRTHSSDSREFGALKKANIEGRTWFIAF
                     PFRDFEFIHKPNYNLSLAPFATKISLALKDLTN"
     gene            complement(3182..4333)
                     /gene="der"
                     /locus_tag="VGE59_02660"
     CDS             complement(3182..4333)
                     /gene="der"
                     /locus_tag="VGE59_02660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY1074579.1"
                     /translation="MNNEHRPIIALVGRPNVGKSTLFNRLIGRREAITSFIAGTTRDR
                     NFGVAEWFGNSWSIVDTAGVLFEDEEDEIEQAQLQTAMEEQVALAIEEATLVALVVDV
                     KEGLHPTEKKLINELRKHNKPIVVLANKSDNAAISTQADVFHELGIDTLFAVSGIHGS
                     GVGEFVDYLIENYPYEGSDESRGLPRVTFIGRPNVGKSSLLNRILGEDRAVVSPVAGT
                     TRDNVKEEATLPNGQKFMFIDTAGIRRRGSIEAGIEKFSLFRTIRAINQSDIVVVVLT
                     IEERPTRGDAHVVMYALEANKDVILVLNKIDRAQQNIMRLSTNERKRLGEKFLRRFVF
                     MQRLPYYFISALDGSGTKEFVNVLTEAIKKHTKSTDFAPQNAEVDEIEE"
     gene            4400..5968
                     /locus_tag="VGE59_02665"
     CDS             4400..5968
                     /locus_tag="VGE59_02665"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074580.1"
                     /translation="MQYAQIIADHKAGYEPFTYQIPTPLLAHLAKGSVVLVPVGRETV
                     HGVVVDFIRRVDDTLAPKLKPIESVVYPGAFIPGYLIDAVYELKQKYGYGLSATLFCF
                     LPEFPKRKQSEIISSGKPGRGYKVHESIIDLPRRADVYRNLAKRVAQSGQSLLIITSS
                     HNATEALCDKLKVPFTLFPADPSAKSRREYFLHALSAEPTIFIGTRGAIATPLNRIGA
                     VVIDEPWLPGHKEDSSPKMWSALAMQAVCKMRGIPLYLISSVAWPESQLLHARQSVIQ
                     TSTPGELTIVPKRPLTEIVTQFLADHDDAHATLAVFVHETHRPVYWCATCERAWPESG
                     TCQKCRKETIMLPPITCDTLHKLLIPHVPDAHITVLPAEALAQYRTFTAALVINFDVY
                     LSITDFRAPSYLRTLITLLRAQAKHVQLVTAHPDTWTPLTQPFDFDAQLVERQTHNLP
                     PFSWAVTLSGKQKPTLEKLDLNEAPDILKASKIHKRRDEYCISLLVKTTGHLPNAWHK
                     KSGLKIDYLPNYVE"
     gene            complement(6043..6618)
                     /locus_tag="VGE59_02670"
     CDS             complement(6043..6618)
                     /locus_tag="VGE59_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074581.1"
                     /translation="MLTSCAVALLEGDWEKLLTYYQKVWPYQSVQSAYIYQLQGVVFD
                     GPGKGSRFHHFLDISGGGVCLGYYPGGLEAAIGTFCPDNVPYLDRKVLEFLRGLETLD
                     PHRGVERYELTIQEVLLPSRVTVGGLLHCLEAYDRTLQELDQVRSRPEWPTDERLICE
                     EVEGLFRSVGRFCSDQSIILAEVVTDWLFED"
     gene            6740..>7306
                     /gene="def"
                     /locus_tag="VGE59_02675"
     CDS             6740..>7306
                     /gene="def"
                     /locus_tag="VGE59_02675"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY1074582.1"
                     /translation="MQSPLVYYPNELLKKRSSEVREFTSELKTLSEHMRQVMKDYAGM
                     GLAAPQVGQSIRLITVEYQPVEGDKNPAIPFTVMINPEITKMGKETDWLSEGCLSIPY
                     VEVDIERPTEVSVTYQDLDGTKQTIHAKGLFARIVQHEIDHLNGTLILDYEKPAEVSD
                     KPRVIFWGSTPFSTEVMNVIRPYVTMVAI"
BASE COUNT         1970 a         1932 c         1662 g         1742 t
ORIGIN      
        1 cgctaaagct agcaagatac aaagccgtgg caataaccgc cgaaactccg actgaaatcg
       61 taaaaaacgg actatgcaca aaaatacggt taatcgcttc ggccgagaaa aaagagctcc
      121 aatccataca tttactatac ccaaaaacgg ggctcgaacc aaatcgttgg cattttacgc
      181 aaaaagtatg cggttccgtc tcttttttta tgcttgctgc tcatagttca ctaccctaca
      241 atggggtaaa tataaggagc acatatatga acatgtacga tttcacgttt tttgctgtag
      301 gggttgcctc ggtccttgtt tccattacac tgatagccct attcatctgg ggaataatgg
      361 taatgaagcg tttgaaccga ttattcgaca cactcgaagc aattagcaag tctggcctcg
      421 agacaacgca agccattcac ggttttgccg aaaaaacgac ggaacaactt tccatggcaa
      481 ttaaaacaat ggtgagcatt caaggggcca aggaaatcgt gggtgcgatt ggagagcaat
      541 tcaagaaaaa caaaaatgag aaggagtctc accatggaac aacaaaataa taaacgtgga
      601 agtatgatgg gagccgccct gttaggggct gctctcggcg ctgcggctgg cctcctattc
      661 gcccctaaat cgggcaaaga aacccgacaa gacctcacag acctcatggg tcgaatgagc
      721 aacgaaatcg gcgaccggac gagcaaacta cgagaagtta ctaaatcggc ttataacgaa
      781 attgtcgatg tggttgtgaa gcagtaccaa gaagctaagg aaatcaccac tgaacaagca
      841 gccaagctac gggaagaact ccgccaaggg tacgagcggg taaaagccgt ggccgacgag
      901 cacaaacttc cagaggggac ttctgtcgaa gagagcccaa aacaaaagag caagaaataa
      961 gagcagccgg cggttccccg ccggctttct cgaagatgaa gataaaggac gcgacagaag
     1021 ccgctacccc atcgcgagat gccgatagag aatcggtcgc atgaacgtta gagcacgctt
     1081 ctcagcctgt gaaactgctt gttttgtcat acggagcacc tctgcagccg caagttgggt
     1141 gtgaggagga caacccaatc cgtaacgaag ccgaataacg tctcgccact ttttcttagg
     1201 tagacgtccc caagcgtact gaactacatc aacagtgacg cttcttccca ccgtatcaaa
     1261 cggatgaggt tcatcacacg gcacaacatc gacaaagaag tcaggtgatt gatcttctgt
     1321 taccggctca tctagggagt atacacattc acctaccccc aaaagagctg cttcgagttg
     1381 cccactgttc aggcctgtcg cggatagcat ttcctcccag gtaggagagc ggtgctcaga
     1441 ctgatacgtc ttattccaga gggtagcaag cttcgctctg gcttcgtgaa tgtgaacagg
     1501 cacacgaatg actgagtcgc cgttctcctt tgcccgtttc atcgcctgac gcacccacgg
     1561 agtgacatag gtagaaacac gagtgcggta ggacttcttg tacttccgaa aaccgtggat
     1621 caacccatca attccgtgcc ccactaaatc ctcatacgac agtgagttac agtgaggttc
     1681 gaccttgccg gcttctgccc ggacaagaca gatgaagcta gcaatcaatc gctcgagaat
     1741 ctggcgattt cgaccgcgct gccactgcag tactaactct ctctgttcct ggtaggataa
     1801 aaagagagga gcaatgacga gagggttgtg aggagcgaga agttctaaag caagcatatc
     1861 tttcccctac tcgaccattc gagcacgcgc ttattataat ctatgtgaga gcaaagagac
     1921 aaccctttgg agaccttgcc ctagccagca caaaaattgt gtgggatgat aggcttacta
     1981 tgttttcatt tatccaatcc gttgttatta ctgttctctt ggcattctta cttcgtttct
     2041 ttgtactcca accttttatt gtcgaaggtt cgagcatgga acctaatttt catgaccagc
     2101 agtatattat tatcgacaaa ctgtcctacc gcatgcgtga gcctcatcgt ggcgaggtga
     2161 ttgtctttca tccaccagtc gctataacgc aaaattatat taaacgaatt attggccttc
     2221 caggtgagac cgttttagta aaagaaggtg aagtattcgt caatggtaaa aaagttgatg
     2281 agccttattt aggcaacgag aaccaccaca ccttacctat cagcacacaa tcccctgtga
     2341 ctctaggaga tgacgaatac tttgtgatgg gagataaccg gacacactct tccgactccc
     2401 gtgagtttgg cgccttaaaa aaggcgaaca tcgaaggccg cacttggttt atcgcttttc
     2461 catttcgtga cttcgaattc atacacaaac caaattacaa cctcagcttg gcaccgtttg
     2521 ccaccaagat ttctttagcc ttaaaagacc tcactaacta gagcacaagc gcctcaccgg
     2581 aggcgctttt ttgatacgta agacttgaca aaataactaa tttatggtat agattaaggc
     2641 atgtatttga ggagcggatg gccgctcatg accgggagag tacgagatgc gattgggaaa
     2701 ggttagtctc ttgagcctga tggttgccct tacggtattt gggctcctcg gcagtgcggt
     2761 tgccgcgatg attggggcac gcccgcatca agtgagtcgg gaaatcaggc tgccggacgg
     2821 ctccgcctcc attagcctcg atgcgaaaag taacaccctg ctcatctgcg gggaccgacc
     2881 cgggcagccc tgctcggaag gcggaagcta cctcgtgaag gatgcatcca atatgaagga
     2941 catccgcaac cttcgctggg gcaaggacgc gtccggactc caactcgtgg cctgggaatt
     3001 cctccgctct gacgatgtgt ggataccagc gagccgaatc cccggcaagc aatggtactc
     3061 gtcatacgag cgagacagcg gacccgagaa gcacttcctc atccgccagg acccatagtc
     3121 gcccacggtg ggcacacaaa acgcccgaga gatcttttag gtctcccggg cgtttgctgt
     3181 tttattcttc tatttcgtct acttctgcat tctgaggagc aaagtccgtc gattttgtgt
     3241 gctttttgat cgcttcggtg aggacgttta caaattcttt cgtgcctgat ccgtctaagg
     3301 cggagataaa gtagtacggc aaccgttgca taaacacaaa acggcgtaaa aacttttcgc
     3361 cgaggcgttt acgttcgttc gtgctcagtc gcataatatt ttgctgtgcg cggtcgattt
     3421 tatttaaaac gagaatcaca tctttattgg cttctaacgc gtacataacc acatgagcat
     3481 cgccacgtgt tggccgctcc tcgatggtaa gcacaacgac cacgatgtca ctctggttaa
     3541 tggcgcgaat agtacggaag agcgagaact tttcgatgcc cgcttcgata cttccgcgac
     3601 ggcggatacc agccgtatcg ataaacataa acttttggcc attaggcaaa gtggcttctt
     3661 ctttgacgtt atcgcgagtt gtaccggcaa cagggctcac tacagcgcga tcttcgccta
     3721 aaatgcgatt caggagggag gacttaccaa cattcggccg accaataaaa gttacacgtg
     3781 gtaatccccg tgactcatcg ctaccttcat atggataatt ttcgattaaa tagtccacaa
     3841 actcacccac cccactaccg tgaatacccg aaaccgcaaa cagggtatca atacctaatt
     3901 catgaaacac atcagcctgg gtactaattg cggcattatc ggatttgttg gctaacacaa
     3961 caataggctt attatgctta cggagttcat taataagttt cttttcagtc ggatgaagtc
     4021 cctctttaac atcaacaacg agagccacga gcgtggcttc ttcgatagcg agggctacct
     4081 gttcttccat agcggtctgg agctgagctt gctcgatttc gtcttcttcg tcttcgaata
     4141 aaacgccagc cgtgtcaacg atcgaccagc tattgccaaa ccactcggca acaccaaagt
     4201 tccggtcacg ggttgtgccg gcgataaacg aagtaatcgc ctcacggcga ccgatcaaac
     4261 gattaaaaag agttgatttg cccacattgg gtcgcccaac cagggcgata atgggacgat
     4321 gctcattatt cataagtata gtatacctga taaacatgtt aggagctagt gttatactac
     4381 caacagagtt attttttcta tgcaatacgc gcaaataatt gccgaccaca aagccggcta
     4441 cgaaccattt acctatcaaa ttcccactcc cttgctcgct caccttgcga aagggagcgt
     4501 ggttctcgtc cccgttggca gagagaccgt tcatggtgtt gtcgtggatt ttatccgccg
     4561 tgtcgacgat accttggcgc caaaactcaa accaattgaa tccgttgtct acccaggagc
     4621 atttattccg ggctacttga tcgacgcggt gtatgagctc aaacaaaaat acgggtatgg
     4681 gctgagtgca acactctttt gttttttacc cgaattcccc aaacggaaac aaagcgaaat
     4741 tataagttcg ggcaaaccag gccgaggtta caaagttcat gaatcaatca tagaccttcc
     4801 tcgaagagcc gatgtttatc gcaatctggc caagcgcgta gcccaatcag gccaaagcct
     4861 cctgattatt accagcagcc ataacgcgac agaggcatta tgcgacaaac tcaaagttcc
     4921 ctttacctta tttccggcag acccatcagc caagtcccgg cgagaatatt ttttacatgc
     4981 cctttccgct gagccaacta tttttattgg tacccgaggc gccatagcca cccccttaaa
     5041 ccggatagga gcggtcgtta tcgatgaacc gtggcttcct ggccataaag aagatagctc
     5101 cccaaaaatg tggtcggcgc ttgccatgca agccgtgtgt aaaatgcgcg gcatccctct
     5161 ctatcttatc tcgtcggtcg cctggccgga gtcacaactg ttgcatgcca gacaatcggt
     5221 tatccagaca tcaacaccgg gagaactcac cattgttccc aagcgtccgc tcaccgaaat
     5281 cgtcacgcag ttcctagctg accacgacga cgctcacgcc actttagcag tatttgtaca
     5341 cgaaacacat cggccggtgt attggtgcgc tacctgcgaa cgagcctggc ccgaatctgg
     5401 cacctgccaa aaatgccgca aggaaacgat tatgcttccg ccaattactt gcgacacatt
     5461 gcacaagctc ctcatccctc acgtccctga tgcacatatt acggttcttc cagccgaagc
     5521 actcgcacaa tatcgcacat ttaccgcggc gctggttatt aactttgatg tctatttatc
     5581 gatcaccgac tttcgagcac cgtcgtattt gcgcacactt attaccctgc ttcgagctca
     5641 ggctaagcat gttcaactgg tcactgccca cccagacacc tggacacctc tgacgcagcc
     5701 gttcgacttc gatgcccaac ttgtagaacg ccaaactcat aatctacctc cgttttcctg
     5761 ggcagttaca ttgtcgggca aacaaaaacc cacacttgaa aagctcgact taaacgaagc
     5821 tccagatatt ctaaaagcca gtaagatcca taaacgccgt gacgaatact gtatctcgct
     5881 cttagtcaaa acgactgggc acctcccaaa cgcctggcat aaaaaatccg gccttaaaat
     5941 cgactactta ccgaactacg ttgaatagag attcctcaca aaataaaaaa gtgggggcgg
     6001 aacgcatggt tccgcccggt tcgccccctc ttacggcctt cactagtctt caaacagcca
     6061 gtcggtaact acttctgcca atatgatact ttgatcactg cagaacctcc caactgaacg
     6121 gaagaggcct tctacttcct cacagattag acgctcatcc gttggccact caggacgaga
     6181 tctgacctga tcaagttctt gaagagtccg atcatacgct tccaagcagt gaagcaaacc
     6241 acccacggtt acgcggctcg ggagtaatac ttcctgaatg gtaagctcat aacgctcaac
     6301 gccacgatgt gggtcgagcg tttcaaggcc acgcaaaaat tcgagaacct tgcgatcaag
     6361 gtaaggcacg ttatcagggc agaacgtacc aattgctgcc tcgaggccgc ccggatagta
     6421 gcctaaacac acgccaccgc ccgagatgtc caggaaatgg tggaaccggg agcccttgcc
     6481 tggaccatcg aacaccaccc cctggagctg gtagatgtac gcactttgga cggactgata
     6541 cggccacacc ttttggtagt atgtcagaag cttctcccag tccccttcca gcagagcgac
     6601 tgcgcaactc gtcaacattc ccccttcctc ctttccgacg ataccagcat cctattccat
     6661 atagtatatt ctcgtcaaca gcgctaaatt caggttcttc tctggctaat ttacccggcc
     6721 tgcggtaaaa tagagccaca tgcagagccc tcttgtctat tatccaaatg aattattaaa
     6781 aaaacgttcc tcggaggtgc gtgaatttac gagtgagcta aaaaccctct ctgagcatat
     6841 gcggcaagtt atgaaagatt acgctggcat gggattagcc gcaccgcagg ttggtcagtc
     6901 gattcgcctc attaccgtcg aataccaacc cgtggaaggt gataaaaatc ccgccatccc
     6961 tttcacggtt atgattaacc ccgagatcac caaaatgggc aaagagactg attggctgag
     7021 cgaaggctgc ctgtctatcc cctatgttga agtcgacatt gaacgcccaa ccgaggtttc
     7081 ggttacgtat caggatttag acgggacgaa gcagaccatc catgccaaag gcttgtttgc
     7141 tcggatcgtc cagcacgaaa ttgatcatct taacggtacc cttattttgg actacgagaa
     7201 acccgcagaa gtaagcgaca agccgcgcgt tattttctgg ggctcaacgc cattttcaac
     7261 cgaagtcatg aatgttattc gaccgtacgt tacgatggtt gccatt
//
LOCUS       DASWYB010000046         7796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_746152, whole genome shotgun sequence.
ACCESSION   DASWYB010000046 DASWYB010000000
VERSION     DASWYB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746152"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..705)
                     /locus_tag="VGE59_02680"
     CDS             complement(184..705)
                     /locus_tag="VGE59_02680"
                     /inference="COORDINATES: protein motif:HMM:NF017345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEY1074583.1"
                     /translation="MPYQEFEGEVPVQSGEIEAGSFRVSSETTPRLIDGVRFLSPEIV
                     VSDSRRTLSVVCNSRDVGMDGQTPLVYVVVIKASTESGKRNFAGNHYHSRRLERIQVV
                     HGCVEVVLCDYRSGAQGRVNILYLSRPDCVIEVPPGVAHAFKEHAGSATLVVHASCDY
                     DPTDDIHVDLTHI"
     gene            1013..2644
                     /locus_tag="VGE59_02685"
     CDS             1013..2644
                     /locus_tag="VGE59_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY1074584.1"
                     /translation="MKSISQRVAENWKSGLTVSLVSIPLSVSLAVASQTSPVVGIITA
                     IWAGFIASLFGGSNFNIIGPTGALSGLLAAYAIAHGMESLAMLAIATGVVTLLAYAFR
                     LERFLVFIPASTIHGFTLGVALIIGLTQLNYAIGLSGLPVHQRLVENILESLNHLSQA
                     SWTTFTVFILFTIGMFACAKLTPKIPGAILLAPLGILLGYLGSNDIIHLPLQTLGMKF
                     SNLNGQLFQAPHFFFNYSLVVTSLTVALVAILETMLSAKIADGMTRTKHNKRKEMLGL
                     GLANIASGLMGGIPATAALARTSLNIKTGANDKLSATVNSIAVAVISLVLLSYFKYIP
                     MAVIAAILVFVAVRMVEVHHFARFYRYDKKSFVVALIVAFVTFYQDPIIGILLGTTIS
                     LLIFVEKLSRGQFDLTANKQEEGIIEHVSGEKITRAFTKSPDTLLYSIKGQLAYINSQ
                     AHITRFERDLSDYKNVILRLKELHFIDLDGVDALDEIIDIIHSQGKTVLLTGVNPYIA
                     TSLHNECHNYKKLEQDGLVFGKISDAFRHLGYGVK"
     gene            2688..2978
                     /locus_tag="VGE59_02690"
     CDS             2688..2978
                     /locus_tag="VGE59_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074585.1"
                     /translation="MTIVATLLSFLGLYGLAAAFDPSCSLDGCGGMAIYALSIGCILL
                     LTSIILFVHERKKIAETGYFDWKEQSVQFIKGLIVMGVISTIAWQVTMYFFR"
     gene            2988..3434
                     /locus_tag="VGE59_02695"
     CDS             2988..3434
                     /locus_tag="VGE59_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY1074586.1"
                     /translation="MARSHTTIDSYLESLPEERREALRKVREVILKYLPEGYEETMDF
                     GMPCYVIPLERFPKTYNGHPLMYAAFASQKNYMALYLMSVYGSPNIEAWFREAFAKAG
                     KKLDMGKSCVRFKKLEDLPLDIIGQTIARVSVENYIAHYEASRNRG"
     regulatory      3485..3585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3619..4842
                     /gene="metK"
                     /locus_tag="VGE59_02700"
     CDS             3619..4842
                     /gene="metK"
                     /locus_tag="VGE59_02700"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY1074587.1"
                     /translation="MSQLSKGGVTVAYEYLLTSESVSEGHPDKMADQISDAILDAFLA
                     QDPQAKVACETLLTNNTVIVAGEINSSATVDVDTIVRDTIRKIGYVGTELGCDAETCE
                     ITNLLHQQSPDIHQGVEQEGEIGAGDQGLMFGYATNETEDLMPLPLWLSHRMVEKQAE
                     LRKNGTLPWLRPDAKSQVTLRYRDGKPVAVERVVLSTQHDPTIDNETLRQAVIEHIIH
                     AVIPAELRAENIEYFINPTGRFVIGGPVGDTGLTGRKIIVDTYGGAAPHGGGAFSGKD
                     PTKVDRSAAYAARYIAKNIVAAGIADRCTVQLAYAIGHIEPMAFSLDFHGTGNVDERE
                     AEAFAKTVFAITPKGIIESLDLRKPRYAATAAYGHFGRQGDSFSWEKTDKTEALKKLR
                     PIALDIAPKKQSAAK"
     gene            complement(4875..5609)
                     /locus_tag="VGE59_02705"
     CDS             complement(4875..5609)
                     /locus_tag="VGE59_02705"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY1074588.1"
                     /translation="MKQSVQVVYAESAAAAKRLAQEELFEYLRHYQGTPMLLLCSGGS
                     SMDLLDNVPDIALGSWLTLGVTDDRMSTDPKVNNFLQLRETAFGQAAEKAQVRFLSTV
                     PRDGQSIDEWSEDFENALREWVNAHPAGKIVMTLGIGRDGHTIGIMPYPEDPLEFARL
                     FTDSERWTVGYDVGTKNPHRLRLTLAPAFLTKHVDHTIVYHVGEEKRQSLLQVLDANG
                     SFADVPGRIVHQLRDVKIVTEERFRE"
     gene            5684..6586
                     /gene="gnd"
                     /locus_tag="VGE59_02710"
     CDS             5684..6586
                     /gene="gnd"
                     /locus_tag="VGE59_02710"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY1074589.1"
                     /translation="MEKTLGIIGLGKMGAGLAQNLADKNWRVVAYNRTHSVTQALASK
                     GVTPTTSIEELIQQLPTPRVVWLMLPAGAVTDEHIDIIAPLLEKGDIVVEGANSYYED
                     AAPRAKRLAEKGITMIDVGVSGGPGGARNGACLMIGGDKATYDTLEPLFKDLARNGTA
                     YQFFPGTGAGHFAKMVHNGIEYGMMQAIAEGFAILKAAPFNYTLTDVTKIYNDGSVIE
                     SRLTQWLESGYTQHGEDLADVSGTVKHTGEGEWTVNTAKKLNIPAPIIEGSFQFRVES
                     EKSPSYTGQVLSVLREQFGGHSVK"
     gene            complement(6628..7683)
                     /locus_tag="VGE59_02715"
     CDS             complement(6628..7683)
                     /locus_tag="VGE59_02715"
                     /inference="COORDINATES: protein motif:HMM:NF020505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monodechloroaminopyrrolnitrin synthase PrnB
                     family protein"
                     /protein_id="HEY1074590.1"
                     /translation="MRMSRYQASWQASRRVALLDPLRADSYFSEVFHSLQMPDAVYDS
                     FSGYVPDRERIAFFDREQAMAATRDLGLVASTIVRWGGQVATYEPLESALLLLSQKTG
                     EIPADTVESYGPRNQAGARTRTYTGTVGERFFIHSFTAAMKELPDVCDLLELARETPL
                     SSPQYAQSLIRAYEGFQAMVQAIVGVHALITPEFFTTILRPYFEPKVVGGKSYYAPGG
                     AQMALTLVDLLVWGAEDDTPEYRHYWRENVEYLPQSVRDNLGTVVRGGSLLRSAERAM
                     AKECPNAHTQRSLEALRRFIVGLEQFRYPHRKVAEANMAIRSPQAVGSGGYTSSILNL
                     LLELTYVARVRVSSLLS"
BASE COUNT         2029 a         2093 c         1809 g         1865 t
ORIGIN      
        1 cggcttgaag ctgctcaggg ttaagtttgg ctttggcctc tataaactgc tgcgtgaggt
       61 gggctcggtt catcgcccgt agtatggcat aatttgtagg tctctgccag ttgcttgctt
      121 acggcaccaa caatagagaa agctgccccg agaggcagct ttacatactt ccaccaaagg
      181 aagctagata tgcgtaagat cgacgtgaat gtcgtcagtt ggatcgtagt cacacgaagc
      241 atggaccacg agcgtggccg atccggcatg ctccttaaag gcgtgcgcca cgccgggcgg
      301 cacctcgata acgcaatccg gccggctgag gtagaggatg ttcaccctcc cctgcgcccc
      361 ggaacgatag tcgcacaaca ccacctcgac acacccgtga acaacttgaa tccgttccag
      421 acgacgtgag tggtagtggt tgccagcaaa gttgcgcttg cctgattccg tagaagcttt
      481 gatgacaacg acgtacacca acggcgtctg cccatccata ccaacgtccc ggctattgca
      541 taccacggac aaggtacggc ggctatcgct caccacaatc tccggactta aaaaccggac
      601 cccatcaatc aggcgtggag tcgtctccga cgatactcgg aaacttcccg cttctatctc
      661 gcccgactga acgggaactt ccccctcaaa ctcttggtac ggcacagaaa actctcctac
      721 acccgaaaca gaggtgcgat gttatttaat cttacttctt cctgcaacac aactttgcgg
      781 gttacactcc ttgcaatccc acatacaaag gtatagaata aatccactta accgtggtgg
      841 tgcactgggt gtgggagcct agtaaatcgc gcggaagagt atgcatactc gccgcccttc
      901 ggggcggcac tttttatagt aaaaatagac tggcctagtg caatctgttg acagcctcaa
      961 aagggaggag aataataaga aaatcgtttt caaattattt atgaggtagt gtatgaaatc
     1021 aatctcccag cgcgtggccg aaaactggaa atctggccta actgtttcgt tagtctctat
     1081 tccactctcc gtttctctcg ccgtagcgtc ccaaacaagt cccgttgtgg gtattattac
     1141 cgctatatgg gctggattta ttgcttcact cttcggcggg agcaacttta atattattgg
     1201 gccaaccgga gcgctctctg ggctactggc cgcatatgcc atcgcccatg gcatggagag
     1261 cttggccatg ctcgccatag ctacaggcgt agtgacactt ctcgcgtacg ccttccgcct
     1321 cgaacgattc ttggttttta ttcccgccag cactattcat ggttttacac taggagtcgc
     1381 actcatcatt gggttaacgc aactcaacta cgccattggc ctgagcggac tccccgtgca
     1441 tcagcgctta gtcgaaaata ttcttgagtc actcaaccac ctctcacagg cttcatggac
     1501 aacctttact gtctttattc tctttacaat cggaatgttt gcctgtgcca agctgactcc
     1561 aaagatacct ggcgcaatcc tcttagcacc acttggcatc ctgctcggtt atttaggaag
     1621 caacgatatt attcacctgc cattacaaac gctcgggatg aagttttcca acttgaatgg
     1681 ccaactcttc caagctcccc actttttctt taattattcg ctcgtcgtaa caagcctcac
     1741 tgttgcattg gtagccatct tagaaacgat gttatcagcc aaaattgccg acgggatgac
     1801 ccgcacaaag cacaacaaac gcaaagaaat gcttggtttg ggattagcaa atattgcctc
     1861 ggggctcatg ggaggaatac cagccacggc cgccttggcg agaacctcgc tcaacattaa
     1921 aactggcgcc aacgacaagc tgtccgccac cgtgaatagc attgctgtgg ccgttatctc
     1981 gctagttctt ctttcttact ttaagtacat tcccatggct gtgatcgctg ccattcttgt
     2041 ctttgtggcg gtgagaatgg tcgaggtaca tcactttgcg cgcttctatc gctacgataa
     2101 aaaaagtttt gtggttgcgc tcatcgtggc atttgtgacc ttctaccaag atccaattat
     2161 tggaatcttg cttggtacca ccatttccct gctcattttt gttgaaaaac tttcacgcgg
     2221 acagttcgac ctcaccgcca acaaacaaga agagggaatc attgagcatg tatccggaga
     2281 aaaaattacc cgggcattca caaagtcacc cgacacatta ctttactcta taaaaggcca
     2341 gctcgcatat atcaatagtc aggctcacat tactcgattc gaacgagatc tcagtgacta
     2401 taagaacgtg atcctacgcc tgaaagagct ccattttatt gacctcgatg gcgtcgacgc
     2461 tcttgatgag attatcgata ttatccactc tcaaggcaaa accgtccttc tcacaggcgt
     2521 gaacccgtat attgccacct ccctccacaa tgaatgtcat aactataaaa aattagagca
     2581 ggacggactt gtctttggga agatcagcga cgctttccgg cacttaggct acggtgtgaa
     2641 ataaggatga caccctactc atctcctacc tgtgatacga ttaccctatg acaatagttg
     2701 caacactcct ctcttttttg gggctctacg gactcgccgc cgcctttgat cctagctgct
     2761 ccctcgacgg gtgtggagga atggccattt acgccctgtc tattggttgc atcctacttc
     2821 ttacttcgat tattctattc gttcacgagc gtaaaaaaat tgcagagaca ggttactttg
     2881 actggaaaga acaatccgtt cagtttataa aaggactcat tgttatgggg gtgataagca
     2941 caatcgcctg gcaagtcacc atgtacttct ttcgctaaga accatctatg gcccgttcac
     3001 acaccaccat tgattcatat ctagaaagtt tacccgagga gcgtcgcgaa gcgctcagaa
     3061 aggtacgcga agttatctta aagtatcttc ccgagggata cgaagagacc atggactttg
     3121 gcatgccgtg ttatgttatc cctcttgaac gttttcccaa aacctacaac ggacatcctc
     3181 ttatgtatgc ggcctttgct tcgcaaaaaa attatatggc cctttatctt atgagtgtct
     3241 atggcagtcc taacatcgaa gcctggtttc gagaagcttt tgctaaagcc ggcaagaagc
     3301 tagatatggg aaaaagctgt gtccgattta aaaagctcga agatttacct ttagatatca
     3361 ttggacaaac catcgcccgt gtttcagtgg agaattacat tgcccactac gaagccagcc
     3421 gtaaccgcgg ctaatatcat ttgacatgtc tcaaagattt ggttacggta agaacaataa
     3481 aatctcatta tcaagagtgc agcgagggac ctagcccaac gacctgccga caacctactc
     3541 tttacgagca aggtgccaca tctagcccga aaggggagat aaccgcaacc tcttccttct
     3601 cggaggagtt ttttttaaat gtctcagtta tcaaaaggag gagttaccgt ggcatacgaa
     3661 tacctgttaa catcggagtc tgtttctgag gggcatcccg acaagatggc agaccaaatt
     3721 tccgacgcaa ttcttgacgc attcctagcg caagaccctc aagctaaagt tgcctgcgaa
     3781 acactactca ccaataatac ggtgattgtg gcgggagaaa ttaatagcag cgcaacggtt
     3841 gatgtcgata cgattgtgcg cgacacaatt cgaaagattg ggtacgtggg aaccgaactt
     3901 ggttgcgatg cagaaacgtg tgagatcacg aacctactcc accaacaatc ccccgacatt
     3961 catcaagggg tagaacaaga aggcgaaatt ggagccggcg accaaggcct catgtttgga
     4021 tatgcaacca acgaaaccga agacttaatg ccgcttcctc tctggctttc acaccgcatg
     4081 gttgagaaac aagctgagtt acgcaagaat ggaacattgc cctggctccg tccagacgct
     4141 aagtcccaag tcacactcag gtatcgagac gggaagccag tcgccgtgga acgcgttgta
     4201 ctctcaaccc aacatgaccc aaccattgat aacgaaaccc tccgacaggc cgttattgaa
     4261 cacattattc atgccgtaat tcccgctgaa ctccgagcag aaaatattga atactttatt
     4321 aaccctaccg gacggtttgt cattggaggg ccagtcgggg acacagggct tactggccga
     4381 aagattattg ttgatacgta cggtggggca gcgcctcatg gcggcggagc gttttccggc
     4441 aaagacccaa caaaagttga ccggtcagct gcatacgccg cccgctacat cgcaaaaaat
     4501 attgtggcgg caggcattgc cgaccgttgt accgtccagc tcgcttacgc catcggccac
     4561 atcgaaccta tggccttctc gcttgatttc cacggcacgg gtaacgtcga cgaacgagaa
     4621 gccgaggcgt ttgccaaaac tgtttttgcc atcacaccga aagggatcat cgaatctctc
     4681 gacttgcgca aacctcgtta cgctgccaca gcggcgtatg ggcactttgg gcgacagggt
     4741 gattcgttta gctgggaaaa gaccgacaaa accgaggcac ttaaaaagtt acggccgatc
     4801 gcacttgata ttgctcccaa aaagcagtca gccgcaaaat aaacaaagag gccgcctggt
     4861 agcggcctct ttgtttattc gcgaaatcgc tcttctgtca ctatcttcac atcacgtagt
     4921 tgatgcacaa ttctccccgg gacgtcggca aaagaaccgt tggcatccag tacctggaga
     4981 agtgactgcc gtttttcctc tcctacatgg tagacgatcg tatggtcaac atgtttagtt
     5041 aaaaaagccg gggcaagcgt aagacgtagg cgatgaggat tttttgttcc cacatcatac
     5101 cccactgtcc agcgttcaga atcggtgaaa agccgggcaa attctaatgg gtcttcgggg
     5161 tacggcatga tgccaatggt gtggccatcg cgaccaatcc ccagcgtcat tacaatcttt
     5221 ccagcggggt gagcgttgac ccattcgcga agtgcatttt caaaatcctc ggaccactcg
     5281 tcgatagact gtccgtccct gggaacagtt gaaagaaatc gtacctgagc tttttctgcc
     5341 gcctggccga aagccgtctc cctaagttgg aggaagttat ttactttcgg atcggtactc
     5401 atccggtcat ccgtgacccc aagcgttagc cacgagccaa gcgcaatgtc tggcacattg
     5461 tcgagcaagt ccatcgacga cccgccagaa cacaaaagca acatgggggt tccttgatag
     5521 tggcgaaggt actcaaaaag ttcctcttgc gctaatcgct tggccgctgc cgccgattcg
     5581 gcataaacta cctgcacaga ctgtttcatt acttccttgt ataccataca tccaagcgca
     5641 gcaagaacat atgatataac aagagaaaga gaggaagcgc accatggaaa aaacacttgg
     5701 cattatcggc ttaggaaaaa tgggagctgg actggctcag aatttggccg ataaaaactg
     5761 gagggttgtc gcgtataacc gtacccactc tgtcacccaa gccttagctt caaaaggggt
     5821 gacacctacc acttcgatcg aagaactcat tcagcagctt cctacgccgc gagttgtatg
     5881 gctcatgctc cccgccggag cagtgaccga tgaacacata gatattattg cccccttact
     5941 cgagaaaggt gatattgtcg ttgaaggggc aaactcctat tacgaagatg ctgctccccg
     6001 cgcaaagcgg ctggccgaaa aaggtattac catgatcgat gttggcgtct ctggcggacc
     6061 aggcggagcc cgcaatggtg cctgcctcat gatcggcggc gacaaagcaa cttacgacac
     6121 actcgaaccc ttatttaaag atttagcccg taacggaacc gcctaccaat ttttcccggg
     6181 aactggcgcc gggcactttg ccaaaatggt tcataacggc atcgagtacg gcatgatgca
     6241 agcgattgcc gaaggctttg ccatccttaa agcggctcct tttaactaca ccctcaccga
     6301 tgtgacaaaa atctataacg acgggagcgt cattgaatct cgtctcaccc aatggctcga
     6361 atctggctat acgcaacacg gcgaagacct ggccgatgtc tcagggacgg taaaacatac
     6421 cggagaaggt gagtggaccg tcaacacggc aaagaagctg aacatccccg ctcccattat
     6481 agaaggctcg ttccagttcc gggtcgaatc agaaaaaagc ccaagctaca caggacaagt
     6541 cctgtcagtc ctgcgcgaac aattcggcgg tcattctgtt aaataaaaaa actggggcgc
     6601 tgcatgcagc gccccttgtc gtctccttta ggagagaaga ctactcacac gtacgcgggc
     6661 aacgtaggta agttcaagaa gaagattgag aattgacgac gtgtaaccac cacttccaac
     6721 cgcctgtggt gatcgtattg ccatgtttgc ctcagccact ttacgatgcg ggtaccgaaa
     6781 ttgctccagc cccacaataa agcgccgcaa ggcctcgagt gaccgctgag tgtgcgcgtt
     6841 tggacattct ttcgccatag ctcgttcagc gctccgcaga agagatcccc cacgaaccac
     6901 cgtaccgaga ttgtctcgta cagattgagg caggtactcc acgttttccc gccagtaatg
     6961 gcggtactct ggagtatcat cttcggcacc ccacacgagc aagtcaacaa gagtgagtgc
     7021 catctgcgcc ccgcctggag catagtaact cttcccacca accactttcg gctcaaaata
     7081 aggccgtagg atcgttgtga agaactcggg agtaattaac gcatgaactc caacaatggc
     7141 ctgaaccata gcttggaatc cttcataggc gcgaatcagc gattgcgcat actgagggga
     7201 agaaagcgga gtctccctcg ccagttcgag caggtcacat acgtccggaa gctctttcat
     7261 cgcagccgtg aagctatgaa tgaaaaaacg ctcgccaacc gtcccggtat aagtacgagt
     7321 acgtgcgccg gcttggttcc gcggcccgta cgattccacc gtgtctgccg gaatttctcc
     7381 cgtcttttgg ctaagcagca gcagtgcgct ttcaagcggc tcataggtcg ctacctggcc
     7441 gccccaacga acaattgtag aggcaactaa cccaaggtct cgggtcgctg ccattgcctg
     7501 ttccctatca aagaaagcaa tcctttctcg gtctggtaca tacccagaaa acgaatcata
     7561 aaccgcatct ggcatctgga gactatgaaa tacctccgag aagtaactat ccgccctgag
     7621 cgggtctagg agcgcaacgc gacggctcgc ctgccagctc gcttggtacc tcgacatacg
     7681 catgttaagt ctccttgcat accaacaggt tgcgcttagt atatcaaaat atactgaaac
     7741 tgtcatacat atctcgaatt tacgtcctct tctgtctcgt atataataag cccatg
//
LOCUS       DASWYB010000047        20785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_488212, whole genome shotgun sequence.
ACCESSION   DASWYB010000047 DASWYB010000000
VERSION     DASWYB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20785
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488212"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VGE59_02720"
     CDS             complement(<1..1417)
                     /locus_tag="VGE59_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074591.1"
                     /translation="MDTTKTLPLRYFLRYFKKYSLGVAAAFVFLILAKVGATLEPLYL
                     KRIIDALTSRSAAEALQQILILYFGIKLLSIFFEFLRDYIFSPVVMGVSRDIEVSVFD
                     HLLKLPVSYHADQKSGAAARAIARGSRAITFVLDFSVSQILPPVFELAFVTFLLLRLY
                     SWEFATITLGTVILYGWFTIWSTDKRQKYRIEGNVKDDQSSGILVDAVTNMDTVKYFN
                     SERKQFSLFTKIKDEWFHLMVRNNRLFSLIYGVQGLILTSGLGWILILAVQRVTSGTM
                     TVGDLVLVTVYIARLSGPITTLGFVYGQFKNSFADLDAMGQILSQEVTIPEPAHPKAL
                     KDPQGTIQFDNVSFGYIGREAVINNLNLEVKSGQKVAFVGSSGAGKSTIAKLLFRLYD
                     VKEGAIVIDGVDLRDLSSETRRKVFGIVPQEPALFNDTIANNIKFGKPEATHEEVVAA
                     AKAAQIHTFIESLPEGYNTLVG"
     gene            complement(1446..1961)
                     /locus_tag="VGE59_02725"
     CDS             complement(1446..1961)
                     /locus_tag="VGE59_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074592.1"
                     /translation="MQLPTETPTDNYFRGDPEHPSHLSVGAVLLSGDKICCHHFQFAD
                     FYILMRETVEPNETFEQALTRGLLDEFGARAKVRAYVGSLKTYYELDDKTVEKTTLYF
                     LCELESRSEAWRKYDDHEKDSTIECHPISFLIPKMKEQAQRLNRTHLDESYILERAQA
                     FLPKDHSHQVV"
     gene            complement(2118..2420)
                     /locus_tag="VGE59_02730"
     CDS             complement(2118..2420)
                     /locus_tag="VGE59_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074593.1"
                     /translation="MKVPETRSWAVREIERLEEEWTFACSDWGGMLELLEARGRGEIT
                     KLQYRYNGSCPFGHYVYRFKVTEAFTLRTLFNFTCDYFPDQWEERRKWHTWGQTSS"
     gene            complement(2488..4320)
                     /locus_tag="VGE59_02735"
     CDS             complement(2488..4320)
                     /locus_tag="VGE59_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074594.1"
                     /translation="MSKKKYSYFRNIGRVIGLNWEVRRWMFLFEIGLNLVQGIVPLLS
                     AYFSGRVLALLATASTGTVVSTSLILNYVVATAAILLFERLSNYFYNYLDDYNSIEFN
                     IVTQRRLMEKFLSLDFTYYEDSGFNDTLEKIDQNRHVISRVTTQLMSLFRNSVQIISS
                     GFAIATLNPWTMAVVAISLIPNLYFELAASLERWKQWRSKGVNIRKTSMIRSNILNSD
                     KVGEIKVFGLENYFLDLWNTYVRKFRGEEVELERKVTRNRSLSSIFEQSAETGIDIWL
                     VLKVLRSAGTFGIGDFQFYRSVIRNFSGACSGFMMSIQGIQERMLYVNDYFSFFELEP
                     RLKAIDTTIVLPDTVPSIEFHNVSFAYPGTEKNVLENFNLVINPGESVALVGKNGAGK
                     STFVKLLMRFYDVSSGEILINGINIKEVNLQSWYTHVGILFQDFSRFEFLNAKETIEI
                     GRTKVKNRDERLEQSIQYSQAHEVISKLPKGLEQTLTKALPEGVGLSGGQWQRLALAR
                     AFYRHAAVLIMDEPTSALDAQAEYNIFQQLNEHQKGKTTLFISHRFSTVRNADKIVVV
                     ADGKIEEQGTHKALMQIPDGTYHRMYQLQAKSYQDTEPEELLTE"
     gene            complement(4350..4835)
                     /locus_tag="VGE59_02740"
     CDS             complement(4350..4835)
                     /locus_tag="VGE59_02740"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1074595.1"
                     /translation="MNTYYQATPESPYHLSAGAVLINDKNEVLAHHFTDFRGFDSFYH
                     IMNETVESGETLEQALHRGLLEEYGATGEIVTYLGSYQSNFHEDGYDKNKTTIYFLVH
                     CISQSEDARLKGDPEGDTKLEWLHIDFLIEEMKKQKLSIREDIDQSLALERAKSYLER
                     K"
     gene            complement(4899..5222)
                     /gene="rpsJ"
                     /locus_tag="VGE59_02745"
     CDS             complement(4899..5222)
                     /gene="rpsJ"
                     /locus_tag="VGE59_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY1074596.1"
                     /translation="MAKKTADQQKIRIRLKAYDYRLIDKTAKTILDTAERTGAVVSGP
                     IPLPTEKKLTTVLRSTFVHKDSREQFEMRIHKRLIDVLDPTAKTIDSLTNLDLPAGVD
                     IEIKM"
     gene            complement(5305..6486)
                     /gene="tuf"
                     /locus_tag="VGE59_02750"
     CDS             complement(5305..6486)
                     /gene="tuf"
                     /locus_tag="VGE59_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY1074597.1"
                     /translation="MAEKFERSKPHVNVGTIGHVDHGKTTLTAAITNVQAKAGKAQKL
                     DVNQIDNAPEEKQRGITIASYHVEYESDKRHYAHVDCPGHADYVKNMITGAAQMDAAI
                     LVVSAPDGPMPQTREHILLAKQVGVPQIVVFLNKCDMVEDEELLELVEEEVRDLLKKY
                     GFPGDETPIIRGSALKALEGDAKYEGKIGELMEALDNYVNDPVREIDKPLLMPVEDVF
                     SIKGRGTVATGRIERGMVKVGEEVEIVGINPTRKTVVTGVEMFRKLLDSGQAGDNVGL
                     LLRGVERTDIERGQVIAKPGSITPHTEFEGEVYILTKDEGGRHTPFQKGYKPQFYIRT
                     TDVTGEVELPAGTEIVMPGDTVTFKVTLIQPIAIEEQMRFAIREGGRTVGAGVVTKIV
                     K"
     gene            complement(6834..6989)
                     /locus_tag="VGE59_02755"
     CDS             complement(6834..6989)
                     /locus_tag="VGE59_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074598.1"
                     /translation="MLLILLIVIEVLSLIFIFLYGYQVKDRSYRKKAYMLFVVSVIQM
                     IWIIFSQ"
     gene            complement(7067..9151)
                     /gene="fusA"
                     /locus_tag="VGE59_02760"
     CDS             complement(7067..9151)
                     /gene="fusA"
                     /locus_tag="VGE59_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY1074599.1"
                     /translation="MAREYSLEKTRNIGIIAHIDAGKTTVSERILFYTGKTYKIGEVH
                     EGAAVMDWMVQEQERGITITAAATTAFWAPTDEPDNKHRINIIDTPGHIDFTIEVQRS
                     LRVLDGAVVVFDAVAGVQPQTETVWRQADKMNVPRICFINKMDRVGADFLKAIESIRV
                     RLLAPAYAAQLPIGQEDKFRGIVDLLENRAILYKDDLGKEWEYAEIPAEIATEAAKYR
                     SELIEKIAESDDALLESYLAGEEPTQEQLQSAMRKAVIAREIFPVYCGTALKNKGVQP
                     LLDAVVHYLPSPLDIPAQMGVNPKTGEEEPRPASDETPMSALAFKIATDPFVGTLSFF
                     RVYSGVVKAGSYVYNSTKGERERIGRIVRLHANSREDVTEVYAGEIAAAIGLKSATTG
                     DTLCDEAKPIILESIVIPEPVISMAIEPKTKQDQEKMGVALQKLAGEDPTFRVSTNHE
                     TGQTLIGGMGELHLDIIVDRMKREFKVEANVGSPQVAYREAIRQEAKGEGKFIRQTGG
                     RGQYGHAVISIKPLERGEGFVFVNKITGGAIPKEFIGPIEKGIKEALERGIVAGYPMT
                     DIEVTLYDGSFHDVDSSEMAFQIAGSMAIQDAVKKADPVILEPVMKVEVITPEDFMGA
                     IMGDMNSKRGRIEQMEDRMGAKIIDAFVPLSEMFGYSTALRSMSQGRASYTMEFDHYE
                     EVPQHVAAKLAK"
     gene            complement(9169..9348)
                     /locus_tag="VGE59_02765"
     CDS             complement(9169..9348)
                     /locus_tag="VGE59_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074600.1"
                     /translation="MQMYLLNRTYIAEDRTTGLMAYSDTERDAQEKLIALVNEYWEKK
                     KVIVPVTKKQENNEA"
     gene            complement(9451..9924)
                     /gene="rpsG"
                     /locus_tag="VGE59_02770"
     CDS             complement(9451..9924)
                     /gene="rpsG"
                     /locus_tag="VGE59_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY1074601.1"
                     /translation="MRGGKPTLKRTILPDERYNSATVAKFINRVMLAGKRETAKNIVY
                     KAMDTLEKATNRPALEAFETALKNASPLVEVRSKRIGGATYQVPMEVRPDRKMALAMR
                     WIINAARDKQGKSMQDFLASELLDAYNSTGTAMKKREDLHKMAEANKAFAHFARF"
     gene            complement(9940..10368)
                     /gene="rpsL"
                     /locus_tag="VGE59_02775"
     CDS             complement(9940..10368)
                     /gene="rpsL"
                     /locus_tag="VGE59_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY1074602.1"
                     /translation="MPTIAQLIRKPRTRVIRKSKSPALHRGFNAQKNTAYNVIEGRPF
                     MRGVCVRVTTMTPKKPNSALRKIARVRLTSGQEVTAYIPGIKHNLQEHSVVLIRGGRV
                     KDLPGVRYHIVRGKLDLAGVQGRAQGRSKYGAKKAAPAKK"
     gene            complement(10485..14279)
                     /gene="rpoC"
                     /locus_tag="VGE59_02780"
     CDS             complement(10485..14279)
                     /gene="rpoC"
                     /locus_tag="VGE59_02780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY1074603.1"
                     /translation="MNGNMSTSSRTPRTTETTQRPTDFKAIRISVASPDQILEWSYGE
                     VTKPETINYRTQKPERDGLFDERIFGPTRDWECYCGKYKKIRYKGVICDRCGVEVTRS
                     SVRRERMGHIDLAAPVSHIWYVRGVPSTLGLVLDLSITDLERVIYFAGFLILEVNQDV
                     KQASLDQLDHEYKDLRAQLIKAHGTPQSPEVLHLDAQFKATKAEIESLQVKKVISEAR
                     YHEISLKYGAIIRVGIGAEAIADLLKEFSIDTEIDMLQKHSQNSQGATLRRVLKRLTI
                     LNSMKAAGINPAWLVLNRLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVLNRNNRLKR
                     LLAQGAPEVIIRNEKRMLQEAVDSLVDNSARRGRGVATGTAQRKLRSLSDMLRGKQGR
                     FRQNLLGKRVDYSGRSVIVVGPKLELNQCGLPKTMALELFKPFVISKLIEEGHVHNVR
                     NATRLIERGTPEVWAILERITVEHYVLLNRAPTLHRLGIQAFHPVLIEGKAIQLHPLV
                     CSAYNADFDGDQMAVHVPLSSQARKEAESIMQSTKNLLKPASGEPIVSPRLDMVFGCY
                     YLTQLETGRVGEGKIFASTDEALMAWQRGIVHLRAPIKVKVKADVVETSMGRLIFNEA
                     LPEELPYVNDTMDAKKLKKLIANVYRNLGQAKTAALVDELKNLGFKFAELSGMTISIS
                     DIVIPQAKPQLIADAEKQQAQITQRYRRGLITARERIMATLTLWQSLRDTLSKQISEE
                     FSPYNPVAIMLNSGARGNITQLSQLGGLKGLVVNPSGEIIEVPITHNFKEGLSVYEYF
                     ISTHAARKGRSDTALRTSDAGYLTRRLVDVAQDVIINTIDCGATEGIVFTRAETDELA
                     MNFGERLLGRVAFEPITLPGEKKPFVEKGEVIDEEKAHKIAEAGVESVTLRSPLYCQD
                     GHGLCRYCYGLDLGTGHLVRRGEAIGIMAAQAIGEPGTQLTMRTFHMGGVSGADITSG
                     LPRVEELFEARMPGEPAILAEIGGRVEITENKETRTVAIYSDKVSVESIEVHDPYEIT
                     VANGDIVKSKQAIATATDRKAIRSPLGGTVSIKGNTVTITALEAMKREYIIPSTVNLK
                     IADGQLVEMGTELTEGHLDLHQSLDLRGPSATQKYIIQGVQEVYASQGQSINDKHIEI
                     IIRRMCAKSKVVDPADSLYLQGQLVDRLEIERVNAERALEKKRPVTIAPSIMGITRVA
                     LKTDSFLSAASFQETSTVLIDAAIRGVVDPLRGLKENVIIGKLIPAGTGHPGWHEDVD
                     LV"
     gene            complement(14282..17608)
                     /gene="rpoB"
                     /locus_tag="VGE59_02785"
     CDS             complement(14282..17608)
                     /gene="rpoB"
                     /locus_tag="VGE59_02785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY1074604.1"
                     /translation="MIERKYFKSFPVTVPLPNLVELQTQSWQWLFNEGIKELFEEINP
                     VTDFTGKALELEFVSYSLDEAKYTEEEAVARNLTYKAPLKAHVVLKNKVTGVTKDADV
                     FLGDFPLQTPRGTFIINGIERVVISQIIRSNGVLFTNEEVAGRNLFGAKIIPSRGAWL
                     EFETSNRDIIAVKVDRKRKIPVTTFLRAFGMEPEAVREAFADVDKDADHKYITTTLDK
                     DTTKSREEAMIEVYKRIRPGDLATAETAQAFLENLFFNPKRYSLGKVGRYKLNQRLNV
                     DVKNVPENQVLRLEDVVAVVKEIITLNNTPDAKADDVDHLKNRRVRAVGELIQARLRI
                     GFARVERIVKDRMSVLEAEEITPGQLINSRPIAAVLQEFFASSQLSQFMNQTNPVAEI
                     EHMRCITATGPGGLARERAGFEVRDVHPSHYGRICPIETPEGPNIGLVSYLASYARVN
                     EYGFIETPYLRVEQKEGKKPRVMDEIVYLDAGEEERHVIAPASTDLTKDGEIAGETII
                     VRKFGEPDIEKALRVNYIDVSARQTVSMTTALIPFVEHDDAARANMGANMEKQAVPLI
                     KPQTPRVGTGMEDAVKNAGQILTAVENGTVTKVDASSITVEGTKGERVYKLQSFVRSN
                     QATCINQRPSVQVGDQVKKGQVIADSYATEQGQLALGQNVLVAYMSFGGYNYEDAIIV
                     SDRLVRDDRYTSIHIEKYSLELRDTKLGPEVLTRDIPNVSEEALANLDDRGVIRIGAE
                     VSAGDIMCGRISPKGETELSAEEKLLRAIFGDKAKDVKDTSLRLPHGERGKVVGVKVF
                     SKDNGDELPASVYMMVEVWVAQMRKLSVGDKLAGRHGNKGVISVILPEEDMPYLADGR
                     PVDIILNPLGVISRMNLGQLMETHLGWAAAELGYYAATPVFDGVTVEQISEELKKAGL
                     PEDGKVQLFDGKTGEAFDQKTTVGINYILKLIHLVDDKMHARSTGPYSMVTQQPLGGK
                     AQFGGQRFGEMEVWALEGYGAAHTLQEMLTIKSDDVTGRSRAYEAIIKGEEIQKPSIP
                     ASFYVLVRELQSLGLNIELIGEDGQISQPQETVTRRVEAKPILELDEEMEDDEEEDVA
                     DIPEDIEVAEPAEAVEAREE"
     gene            complement(18011..19027)
                     /gene="obgE"
                     /locus_tag="VGE59_02790"
     CDS             complement(18011..19027)
                     /gene="obgE"
                     /locus_tag="VGE59_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY1074605.1"
                     /translation="MFHDEVSLSLESGKGGDGAVSFRREKYIPKGGPDGGDGGRGGSV
                     FIEVNTQLTDLNHLASVRELKAESGVPGAGKKLFGRKGEDLTVFVPRGTRVYRQAGTA
                     WRLVADMTEADSRKRVLKGGNGGLGNVHFATATHQTPRYAQPGEPAERAVFKFELQLI
                     ADVGIIGLPNAGKSTFLSIVSDAQPKIGNYPFTTLSPVLGVVPYREKRFVFADIPGLI
                     EGASEGKGLGHQFLRHVKRTKTLVHFIESLSDDYARDYSTVRNELEKFDSGLAQKSEI
                     VVITKSELITDDIAEETQVKIEALKACLSQSSHLFSRHTISAVSRENIKELLAEIGEQ
                     LSEE"
     gene            complement(19105..20178)
                     /locus_tag="VGE59_02795"
     CDS             complement(19105..20178)
                     /locus_tag="VGE59_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1074606.1"
                     /translation="MQQRIGLLYRQVLAFLKQLTQHKSITQTRHYGTQTYHAVREYSR
                     IHQALVVLTIFILVNNTLIVRAANDVPSIPADVLVSDPYVIADTVRLLADYTPDVDED
                     PDAIALALEERVNGNFISTNPLIVTLPSESDTPNATETPAPPASTADRTREIKYTVQI
                     GDTMSGIGSKFGLKVATIKVKNNINNVDTLKPGQELTIPVDDLSDKAIKAAEDRQKAS
                     QQLADAKKSVAKISTAKASGGYGLIVPIHHNGISRGLIAGVHTGIDYRANVGTPIVAA
                     ADGVVIVATETGWNGGYGKTVLISHTGNKTTRYGHMSDVSVRSGQRVSQGELIGYSGN
                     TGRSTGPHLHFELRVGGRAVSPF"
     gene            complement(20325..>20785)
                     /locus_tag="VGE59_02800"
     CDS             complement(20325..>20785)
                     /locus_tag="VGE59_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074607.1"
                     /translation="VYGFNVTVSPQVKKAAENLGVTVTSFKIIYDMLNLIELALKGLV
                     KTEKILVERGRLKVLKVFRTTKEAQIIGGEITQGIAMKKAQISLMRAGEELGKGKVLA
                     LQKGPEAVDELQAPEQCGVSVSVSEKVKEGDMVIFFSEEEVIVNDTAPTE"
BASE COUNT         5246 a         4787 c         4875 g         5877 t
ORIGIN      
        1 cccctactaa ggtgttgtat ccttcaggga gagactcgat aaaagtgtga atttgagcag
       61 ctttggctgc agcgactact tcttcgtggg ttgcctcggg tttaccaaat ttgatgttat
      121 tggcaatggt gtcgttaaat agcgctggtt cttgtggcac aatgccaaaa accttgcgac
      181 gcgtttcact ggaaaggtca cgtaaatcaa caccatcaat cacgatagca ccctctttga
      241 cgtcataaag gcggaaaagg agtttggcga ttgtcgattt ccctgctccg cttgagccca
      301 caaacgcaac tttctgtcca gattttactt ccaaattcag attattaata actgcctctc
      361 gtccgatata cccaaatgag acattatcaa actgtatagt tccttgggga tctttgagcg
      421 ctttgggatg tgcgggttcg gggatagtga cttcttgaga caatatctgc cccatcgcat
      481 ctaagtcggc aaagctgttt ttgaactggc catacacaaa tccaagggtg gtgatgggac
      541 ctgaaagacg ggcaatgtag actgtcacaa gtacaaggtc tccaacagtc attgttccac
      601 tggttacacg ttgtacggca agaattaata tccagccaag cccacttgta agaattaagc
      661 cttggacgcc ataaataagt gagaaaagcc ggttattacg aaccatcaag tggaaccact
      721 cgtctttaat ttttgtaaag aggctaaact gtttacgttc gctattaaag tattttaccg
      781 tatccatatt cgtgacagca tcgactagaa tgccgctaga ctggtcgtcc ttaacattac
      841 cttcgatgcg gtacttctga cgcttatcag tactccaaat ggtaaaccac ccatacagaa
      901 tgactgttcc aagagtaatg gtggcaaatt cccagctata caaacgaaga agtaagaagg
      961 ttacgaacgc gagctcaaac acaggaggta gaatttgaga tacagagaaa tcgagaacga
     1021 atgtgatggc acgcgagcca cgtgcgatcg ctcgcgctgc tgctcctgat ttttggtctg
     1081 cgtggtagct cactgggagc ttgagaagat gatcaaaaac cgaaacctct atatctcgcg
     1141 acactcccat cacaaccggt gaaaaaatat agtcacgtaa gaattcaaaa aatatgctga
     1201 gcagtttaat tccaaaatag agaatcaaaa tttgttgtaa agcttctgct gccgaccgag
     1261 aagtcaatgc gtcaataatt cgcttgaggt aaaggggctc gagtgtcgca cctacttttg
     1321 ccaaaatcag aaaaacaaaa gcggctgcaa cgccaagact gtacttctta aaatagcgta
     1381 aaaaataacg taacgggaga gtttttgttg tatccatgct ctctaggata cacttttttt
     1441 ctgtattaaa ctacctgatg gctatggtct tttggcaaaa atgcctgtgc tcgttctaaa
     1501 atgtaggatt cgtcgagatg tgttcgattt aagcgttgcg cttgttcctt cattttcggg
     1561 attaaaaagc tgatggggtg acattcaatc gtactgtctt tttcgtgatc atcatacttg
     1621 cgccacgctt cacttcttga ttctaattca cacaaaaaat agagggtagt tttttcgaca
     1681 gttttgtcat cgagttcgta gtaggttttg agtgacccaa cataggctcg gactttcgca
     1741 cgggcaccaa attcatctaa aagaccacga gtcagcgctt gctcaaacgt ttcgttcggt
     1801 tcgacggttt ctctcatcag gatataaaag tcagcaaatt ggaaatgatg acagcaaatt
     1861 ttatcgcctg ataagagaac tgcgccaaca gagagatgag atggatgttc cggatcacct
     1921 cggaaataat tatcggtagg agtttcagtc ggtagctgca ttacagtctt attttctcct
     1981 ctcaagattc cggacgcatt aggcgacttc ctatgttttg ttactctgct cgattaattt
     2041 tttgttaata gttacaagga tatctaactc gctcagccac tttaaggatg tcggccggaa
     2101 agttcctctc cggccgacta ggaggacgtc tgtccccatg tgtgccactt gcggcgctct
     2161 tcccactggt cgggaaaata atcgcacgta aagttgaata acgtgcggag tgtaaatgct
     2221 tcggttactt taaaccgata gacatagtgg ccaaacggac aactgccgtt gtatcgatat
     2281 tgcagcttgg taatctcgcc tcggcctcgc gcttccagga gctcaagcat accgccccag
     2341 tcgctgcagg caaatgtcca ttcttcttcg agtcgctcga tttctcgtac ggcccatgac
     2401 ctggtttccg gcactttcat gcgtgctccc cctttatgcg ttatacaaga aaagtaatcg
     2461 accgagaaga cggattattt ttgtgttcta ttccgttagc aattcttccg gttcggtgtc
     2521 ctgataactc tttgcttgga gctgatacat ccgatgatat gtaccgtctg gaatctgcat
     2581 taaagccttg tgtgtcccct gctcttcgat ttttccatcg gcgacaacga ctatcttgtc
     2641 ggcatttcga accgtactaa agcgatggga aataaatagt gtggtttttc ctttttgatg
     2701 ctcattgagt tgctggaaaa tattatattc cgcttgtgca tcgagagctg aggtcggttc
     2761 atccataatg agaacggccg catgccgata gaatgcacga gctaaggcaa ggcgttgcca
     2821 ctgtccgccc gaaagcccaa cgccctcggg gagcgctttg gttaacgttt gttcaaggcc
     2881 tttaggaagt tttgatatta cctcgtgtgc ttgagaatac tggattgatt gttctaatcg
     2941 ctcgtctcgg ttttttactt tggttcgtcc aatttcgatt gtttcttttg cattgagaaa
     3001 ttcaaatcgt gaaaagtctt gaaagaggat accgacgtga gtataccaac tctgtaaatt
     3061 aacttctttt atgtttattc cattaataag gatttctcca ctgcttacat cataaaatcg
     3121 cattaagagt ttaacgaatg ttgattttcc cgctccgttt ttaccaacta gagcaactga
     3181 ctctcccgga ttaatgacca gattaaagtt ttcaagcacg tttttttcgg tacccggata
     3241 tgcaaacgaa acattatgga attcgatact cggcacagta tctggtaaca ctattgtagt
     3301 gtctattgct ttcaagcggg gctcgagttc aaagaaagaa aagtaatcat tcacataaag
     3361 cattcgctct tgaatcccct ggatcgacat catgaatccg ctacacgcac ccgagaagtt
     3421 acggattacc gagcgataga actgaaaatc accaatccca aaggtaccgg cactccggag
     3481 aaccttaagg acaagccaaa tgtcgatacc tgtttcggcg ctttgttcaa aaatacttga
     3541 taaggatctg ttacgggtta cttttctttc aagttcgact tcctcacctc gaaatttacg
     3601 gacgtacgta ttccaaaggt caagaaaata gttttctaaa ccaaatactt ttatttcgcc
     3661 gaccttatca ctattaagga tattgctgcg aatcattgat gtttttcgaa tgttcacacc
     3721 tttactgcgc cattgcttcc aacgttctaa actcgcggcg agttcaaaat acaaattagg
     3781 aattaatgag atcgctacaa ctgccattgt ccagggattt aaagtagcga tagcaaatcc
     3841 gctcgagata atttgaaccg agttgcgaaa aaggctcatg agctgtgtgg tgacccgact
     3901 aataacatgt cgattctgat cgatcttttc gagcgtatcg ttgaacccgc tgtcttcata
     3961 ataggtgaag tccagtgaca gaaatttctc cataagccgt cgctgggtca caatattaaa
     4021 ttcaatcgag ttgtaatcgt ccagatagtt gtaaaagtaa ttcgagaggc gctcaaaaag
     4081 caaaatagct gccgttgcga ccacatagtt taaaatgaga gatgtactta ctactgtacc
     4141 agtggaagca gttgccagga gggcgagtac ccggcctgaa aagtaggctg atagcaaggg
     4201 aacaatacct tgtactaagt ttaaaccgat ctcaaacagg aacatccatc ggcgaacttc
     4261 ccagtttaag cctatcactc tgccaatatt acggaaatat gaatacttct ttttactcaa
     4321 ttatttctcc tcagcgcgca tgtttatgtt tattttcgct cgaggtagct ttttgcacgc
     4381 tcgagtgcca gcgactggtc tatatcctca cgaatggata gtttctgttt tttcatttct
     4441 tcgataagaa aatcaatatg taaccattca agtttggtat cgccctctgg gtcgcctttg
     4501 agacgagcgt cctcactttg agaaatacaa tgaacgagaa aatagatggt tgttttgttt
     4561 ttatcgtacc catcttcatg aaaattactc tggtatgatc caagatacgt tactatctct
     4621 ccagttgcac catactcttc taatagtcct cgatgcaatg cttgttctaa cgtttcgccc
     4681 gattcaactg tttcattcat aatatgataa aaagagtcaa acccacgaaa gtctgtaaag
     4741 tgatgagcga gaacttcgtt tttatcatta attaaaactg caccggctga gagatggtag
     4801 gggctttcgg gagtggcctg ataatacgtg ttcatgtaaa ctcctgctat ttctgtacag
     4861 tataacaaaa acccggagtg attaccgggc ttttgttctt acatcttgat ttcgatgtcg
     4921 acaccagctg ggagatccag gttggtgagg ctgtcgattg tcttggcggt tggatccaag
     4981 acatcgataa ggcgcttatg gatgcgcatt tcaaactgtt cgcggctgtc tttgtgcaca
     5041 aatgttgaac gtaaaacagt agtgagtttt ttctcggttg gaaggggaat tggcccgctt
     5101 actacagcgc cagtgcgttc cgctgtatcg agaatagttt tcgcggtttt atcgattaaa
     5161 cgataatcat atgccttgag gcgaatacga atcttttgtt gatctgcagt cttttttgcc
     5221 attgtttctc cgtaataagg ttcctaaggt tcggtttcta aactactaag agcccccaga
     5281 ggggctcctt agcagttacc aaacttattt aacgatcttg gtaacgacac cagcaccaac
     5341 ggtgcgtcca ccttcgcgga tagcgaagcg catttgctct tcgatagcga taggctggat
     5401 gagggttact ttaaaggtaa ccgtatcacc tggcatcacg atttctgttc cagctggaag
     5461 ttcaacttcg ccggtaacgt cagtagtgcg gatatagaac tgaggcttgt aacccttctg
     5521 gaatggggtg tgacgaccac cttcatcttt ggtaaggata tacacttcac cttcgaattc
     5581 tgtgtgagga gtaattgaac ctggcttggc aataacctga ccacgttcaa tgtcggtacg
     5641 ctcaacacca cggaggagga gaccaacgtt gtcaccagcc tgaccggagt caagaagctt
     5701 acggaacatt tctacacctg ttacaacagt tttacgagtt gggttaatac caacaatttc
     5761 gacttcttca ccgactttga ccataccacg ttcgatacgg ccagtagcaa cggtgccgcg
     5821 acccttaatg gagaagacat cttctaccgg cataaggaga ggcttgtcga tttcgcgaac
     5881 tggatcattg acgtagttgt cgagtgcttc catgagttcg ccgatcttac cttcatattt
     5941 agcatcgcct tcaagagctt tgagggctga accacggatg ataggtgttt cgtctcctgg
     6001 gaagccatac ttcttgagga ggtcacgaac ttcttcttca acgagctcga ggagctcttc
     6061 gtcttcaacc atgtcgcatt tgttgaggaa tacgacgatc tgaggaacgc caacctgctt
     6121 tgcaagcaag atgtgttcac gagtctgagg cattgggccg tcaggggctg atacaaccaa
     6181 gatagcggca tccatttgag cggcaccggt gatcatgttt ttgacgtagt cagcgtgtcc
     6241 tggacagtca acgtgcgcat agtggcgctt gtcggattcg tactcaacat ggtacgaagc
     6301 gatcgtaata ccgcgctgct tttcttcagg cgcgttgtcg atctggttaa cgtcaagctt
     6361 ctgggctttg ccagctttgg cctgaacgtt ggtgatggcc gcggttaacg tggtcttacc
     6421 atggtcgacg tgaccgatag taccaacgtt aacgtgtggc ttactgcgtt cgaatttctc
     6481 tgccatttgg ctcctttctc cacacgtaca gggccttgga ttagggagag atccgcaacg
     6541 ttgatgtgga tctattccct aaccctcgtt ccctacgtaa acgagttttc tacatataca
     6601 agtatacttt cttttacaga gtgagtcaag ctgttgcgta tcttttcccc aggtcggaga
     6661 cgagtctccg ctagtggata aattcatgac aatttaagaa ttttatggta ctatccatta
     6721 ggttgttatt ttatgaaaca atcaaacata ttggccgccc agtgtggcgg ctacccggct
     6781 gaggcgtgaa ggttgcagag gttgctcctt catccgctca gccggttttt ttattattgt
     6841 gaaaatataa tccagatcat ctgaattact gagacaacaa agagcatgta cgctttttta
     6901 cgataggatc ggtctttgac ttgatatcca tacaagaaaa tgaaaatgag ggagagaact
     6961 tcaataacaa taagcaagat aagtagcata cgaattcctt gttttcattg agcataacaa
     7021 aaagtcgctc cattgaggag cggctttttg catcttatgg aatgtattac tttgcgagct
     7081 ttgcggccac gtgctgtggt acttcttcgt aatggtcgaa ttccatcgtg taggaggcac
     7141 gtccctgaga catcgagcga agagctgttg agtaaccgaa catttcgctg agcggtacaa
     7201 aggcgtcaat aatcttcgcg cccatacggt cttccatctg ttcgatacgt ccgcgttttg
     7261 agttcatgtc gcccataatg gcgcccatga agtcttctgg tgtaataact tccactttca
     7321 tgaccggttc gagaataact gggtcagctt tctttacagc atcctgaata gccatcgagc
     7381 cggcaatttg gaaggccatt tctgacgagt caacatcgtg gaaggaacca tcatacagtg
     7441 tgacttcgat atcagtcatt gggtagccag ccacaatacc gcgttcaagt gcttctttaa
     7501 tacctttttc aattgggcca ataaattctt tcggaatagc cccaccggta attttattaa
     7561 cgaagacaaa cccttcgcca cgctctaatg gcttaatgga gataacggca tgaccgtatt
     7621 gaccacgtcc acctgtttgg cgaataaact taccttcacc cttggcttcc tgtcggatag
     7681 cttcacgata cgcaacttgt ggtgagccca cgttggcttc aactttaaat tcgcgcttca
     7741 ttcggtcaac gatgatatcg aggtgaagtt cacccatacc accgataagg gtttgaccag
     7801 tttcgtggtt ggttgaaacg cggaaggttg gatcttcacc agctaatttt tgcagagcaa
     7861 cacccatttt ttcttggtct tgcttggttt ttggttcaat agccatcgag ataactggtt
     7921 cgggaataac aattgattcg agaatgattg gtttcgcttc gtcgcaaagt gtatcccctg
     7981 ttgttgcgga ttttaagcca atagcggctg cgatttcacc cgcatacact tcggtcacgt
     8041 cttcacggga gtttgcgtgc aatcggacaa tgcgtccaat tcgttcacgt tcacccttcg
     8101 ttgagttgta gacataggag ccagctttca ccacgccgga atagacgcgg aagaagctta
     8161 atgttccaac aaatgggtcg gttgcaattt taaaggccaa cgctgacata ggtgtttcgt
     8221 ccgaagcggg acgtggttcc tcttcgccag ttttagggtt tacacccatc tgggcaggaa
     8281 tatccaatgg agaaggcaga tagtgaacaa ccgcatcgag gagcggttga acgcctttgt
     8341 ttttaagagc tgtaccgcag taaactggga agatttcacg ggcaataaca gctttacgca
     8401 tagcgctttg gagctgctct tgagttggct cttctcccgc taagtatgat tcaagtaagg
     8461 catcatcgct ttcagcaatt ttttcgatca gttcggagcg atacttggct gcttcggtgg
     8521 caatttcagc tggaatttca gcatattccc actctttgcc taagtcgtct ttgtaaagaa
     8581 tcgcgcggtt ctcaagcagg tctacaatgc cgcggaattt atcttcttgg ccaattggaa
     8641 gctgcgctgc ataggctgga gcaagaagac gaacgcggat tgattcgata gcttttaaga
     8701 aatctgcgcc cacacggtcc attttgttaa taaaacaaat acgtggaaca ttcattttgt
     8761 cggcttgacg ccatacagtt tcagtctgtg gctgaacgcc ggctacggcg tcaaaaacga
     8821 ctactgcgcc gtcaagaaca cgcaaggaac gttgtacttc gatcgtaaag tcaatgtgac
     8881 ctggcgtatc gataatgtta atacggtgtt tgttgtctgg ttcgtctgtg ggagcccaga
     8941 aagccgtggt tgcagcggcg gtaatggtaa taccccgctc ttgctcttgt accatccagt
     9001 ccataacggc ggcgccttcg tgcacttcac caattttgta cgttttgccg gtataaaaaa
     9061 gaatacgctc agaaactgtc gtctttccgg cgtcaatgtg ggcaataatt ccaatgttac
     9121 gggttttttc tagactatac tcgcgtgcca tattttatcc tttatccctt aggcttcatt
     9181 gttttcctgt ttttttgtga caggtacgat aacttttttc ttttcccagt attcgttgac
     9241 aagcgcaatc agcttttctt gcgcatctct ctcggtatcg gagtaagcca tcagaccagt
     9301 tgtccggtct tcggctatgt acgttcgatt caacaaatac atctgcatct tcatagactt
     9361 aaactcggaa taatttgaaa acaggattca gaggtagttt accaactttt atgcaatccg
     9421 gaaagctggt gctgaatcct gttaattcgt ttagaatcga gcgaagtgag cgaaggcttt
     9481 gttcgcttcg gccatcttgt gaaggtcttc gcgcttcttc atggcggttc cggtgctgtt
     9541 atacgcatcg aggagctcgc ttgccaagaa gtcttgcatc gatttcccct gcttgtcgcg
     9601 ggctgcgttg atgatccaac gcatagctag tgccattttt cgatcggggc ggacttccat
     9661 aggcacctga tatgtcgcgc caccaatacg ctttgatcgt acttctacga gaggtgaagc
     9721 gttcttaaga gctgtttcaa aagcctcgag agcggggcga ttggttgctt tttcaagcgt
     9781 atccattgcc ttgtagacaa tattttttgc ggtttctcgc ttcccggcta acataacgcg
     9841 gttgataaat ttcgcaaccg ttgcactgtt gtagcgttca tcgggaagga ttgttcgttt
     9901 gagagttggt ttaccaccac gcatgcaaaa ctcctaagct tacttcttag ctggggcagc
     9961 ttttttggcc ccatactttg aacggccttg tgcccgtcct tgtacgcccg cgaggtctaa
    10021 tttcccacgg acaatgtgat accgcacacc tggaaggtct tttacacgac cgccacggat
    10081 aagtaccacg gagtgttctt gaagattatg tttgataccg ggaatgtacg ctgtaacttc
    10141 ctgaccagag gttaaacgaa cacgggcgat cttacgaagc gccgagtttg gtttctttgg
    10201 cgtcattgtc gtaacacgga cacacacacc acgcataaat ggtcggcctt caatcacatt
    10261 gtaggctgta tttttctgtg cgttaaaacc acggtggagg gccggagact ttgacttacg
    10321 gatgacacgc gtacgcggtt tacgaatgag ttgagcaatc gtaggcataa atatttcttt
    10381 ctttaccttg ttattgtagc agttctcttg agaaaactca agactttgtt cgatggtaac
    10441 agtggggagt ccggggaatt cccggactct gccactgttg cctattatac gaggtcgacg
    10501 tcttcgtgcc agccggggtg tccggtacca gcaggaatca gttttccaat aataacgttt
    10561 tctttcaatc cacgaagcgg atcaacgacg ccacggattg cagcatcgat aagcacggtt
    10621 gaggtttcct ggaagctggc tgcagacaag aaggaatccg tcttcagagc aacccgggta
    10681 atgcccataa tgcttggcgc aattgtgacg ggacgtttct tttccagagc ccgctctgcg
    10741 ttgacgcgct cgatttccaa tcggtcaacg agttgacctt ggagatagag actgtcggca
    10801 gggtcaacaa ctttggactt ggcacacatt cgtcgaataa tgatttcgat gtgtttgtcg
    10861 ttaatgcttt gtccttgtga ggcgtaaact tcctgtacac cttgaatgat gtacttttgg
    10921 gtggcgcttg gaccacgaag atcgagagat tgatgaagat caaggtgacc ttcggttaac
    10981 tctgttccca tttcgacgag ctgaccatca gcaatcttca ggttgacggt tgaagggata
    11041 atgtattctc gtttcattgc ttcaagagca gtgatagtaa cagtattacc cttaatagag
    11101 actgttccac cgagtggtga gcggatggcc ttacgatcag ttgctgtagc aattgcttgt
    11161 tttgatttta cgatatcacc gttagcaaca gtaatttcgt aaggatcgtg aacttcaatt
    11221 gattcaacac tcactttatc tgagtagatg gcaacggtgc gagtttcttt gttttccgta
    11281 atttcaacac ggccaccaat ttcagctaag atagctggct cacctggcat acgagcttcg
    11341 aagagttctt ctacacgagg cagaccactc gtaatgtcgg cgccggagac gcctcccatg
    11401 tggaaggtac gcattgtcag ctgggttcct ggttctccaa tagcttgggc cgccatgata
    11461 ccgatcgctt caccgcgtcg gactaagtga ccagtaccaa gatcaagacc gtagcagtag
    11521 cggcagaggc catggccatc ttggcagtaa agcggcgacc gaagggttac tgactcaaca
    11581 ccggcttcag caatcttatg tgccttttct tcatcaatga cttcgccttt ttcaacgaac
    11641 ggtttctttt ctcccggaag agtgattggt tcgaaggcaa ctcgcccgag aagacgttcg
    11701 ccaaagttca tagcgagctc atctgtttcc gcacgagtga agacgatacc ttccgttgct
    11761 ccacagtcga tggtgttaat aatcacatct tgagcaacat cgacgagacg acgcgtaagg
    11821 taaccagcgt ccgaggtacg aagagccgta tcggaacggc cttttcgagc ggcgtgggtg
    11881 gagatgaagt attcgtatac cgatagacct tctttaaagt tgtgggtaat cggaacttca
    11941 atgatttcac cggatgggtt aaccaccaga ccttttaagc ctccaagctg cgagagctga
    12001 gtaatgttac cacgagcacc cgagttaagc atgatagcca ctgggttgta tggtgagaat
    12061 tcctcgctga tctgcttgga cagtgtgtca cggagtgact gccagagcgt cagggttgcc
    12121 atgatacgtt ctcgagccgt gattaaacca cgtcggtaac gctgtgtaat ctgagcttgt
    12181 tgcttttcag cgtcggcaat aagttgtggc ttggcctgcg gaatgacgat gtcactaatc
    12241 gagatggtca tccctgaaag ctcagcgaac ttaaagccaa ggttcttcaa ttcgtcaact
    12301 aaggcggcgg tttttgcctg gcctaagtta cggtagacgt tggcaatcag tttcttaagc
    12361 ttcttcgcgt ccatcgtgtc attaacatat ggcagttcct caggaagagc ctcgttaaag
    12421 atgagtcgac ccatcgatgt ctcaacaaca tcagctttaa ctttcacttt gattggagcg
    12481 cgaagatgga caatgccacg ctgccacgcc attaaggctt cgtctgttga ggcaaaaatt
    12541 ttgccttcac caacacggcc tgtttcaagt tgagttaagt agtaacagcc aaaaaccatg
    12601 tccagacgtg gtgatacgat tggttcaccc gaagcaggtt tgaggaggtt tttggtcgac
    12661 tgcatgatcg actctgcttc cttacgcgct tgcgaagaaa gtgggacgtg aacagccatc
    12721 tggtctccgt cgaagtcagc gttgtaggct gaacaaacga gtgggtgaag ctggatagct
    12781 ttgccttcga tcagaacggg gtggaacgcc tgaataccca aacggtgcaa ggtaggagca
    12841 cggttgagta acacgtagtg ctctacagta atgcgctcga gaattgccca cacttcaggg
    12901 gtgccgcgtt caattaagcg agtggcattt cggacgttat gaacatgtcc ttcttcgatc
    12961 aatttcgaaa tcacaaacgg cttgaagagt tcgagtgcca ttgtcttagg cagaccacat
    13021 tggttgagtt cgagttttgg accaacaacg ataactgaac gacctgagta gtcaacacgc
    13081 tttccaagca agttctgacg gaagcgtcct tgttttccac ggagcatgtc ggaaagcgaa
    13141 cggagcttac gctgtgcggt acctgttgca acaccacggc cacggcgggc tgagttgtca
    13201 acgagtgagt cgacagcttc ctgcaacata cgtttctcgt tacggatgat aacttctgga
    13261 gcgccctgtg ctaacagacg ttttaagcgg ttgtttcggt taagaacacg acgataaagg
    13321 tcgtttaagt cggatgttgc gaatcgtcca ccgtcaagct gaaccatcgg acgaagatcc
    13381 ggtggaataa cgggcagacg gtttaagacg agccaagcag gattaatgcc agctgccttc
    13441 atggaattca gaatcgtgag gcgttttaaa acgcggcgaa gtgtcgcacc ttggctgttt
    13501 tgtgagtgct tttggagcat atcgatttca gtgtcaatcg agaactcttt taagaggtca
    13561 gcgatggctt ctgccccaat accaacacgg atgattgcac catacttgag tgaaatctca
    13621 tggtaacgcg cttcggagat aactttttta acctgcaggc tttcgatttc tgccttggtt
    13681 gctttaaatt gtgcatcaag gtgaagaact tcgggtgact gaggggttcc atgggcttta
    13741 atgagctgag cacgaagatc tttgtattcg tgatcaagct gatctaagct ggcttgtttg
    13801 acgtcttggt ttacttcgag gattaagaat ccagcaaagt aaataacgcg ttcaaggtca
    13861 gtgatcgaca aatcgaggac taagccaagc gtcgatggca cgccacggac ataccagata
    13921 tggctaacag gtgcagcaag atcaatatgg cccatacgtt cacgacgaac cgaagaacgt
    13981 gtcacttcaa cgccacaacg gtcacagata acacccttgt agcggatctt tttatactta
    14041 ccacagtaac attcccagtc gcgggttggc ccaaaaatac gttcgtcaaa cagaccatcg
    14101 cgttctggct tctgggtgcg gtagttaatg gtttctggct tcgtaacttc accgtatgac
    14161 cattcaagaa tctggtcggg gcttgcaact gaaattcgga ttgctttgaa atcagttggt
    14221 cgctgggttg tttcggtggt acggggagtg cgggatgatg tgctcatatt tccgttcatg
    14281 cttactcctc tctcgcttca acggcttctg ccggttcggc tacttcgatg tcttctggga
    14341 tatcagcgac gtcttcttcc tcatcgtctt ccatttcttc gtcgagttcg agaattggtt
    14401 tcgcttcaac acgacgggta acggtttctt gaggctgcga aatttggccg tcttcaccga
    14461 taagttcaat atttaatcca aggctttgaa gttcacgtac aagcacgtag aacgaagctg
    14521 ggatcgatgg tttctgaatc tcttcacctt tgataatcgc ttcataggcg cgtgagcggc
    14581 cggtgacgtc atcggacttg atagtgagca tctcttggag ggtgtgagct gcgccataac
    14641 cttcgagagc ccagacttcc atttctccaa aacgctgacc gccaaactga gcttttccac
    14701 ccaatggctg ttgtgtgacc atggagtatg gaccagttga acgagcatgc attttgtcgt
    14761 caacgaggtg aataagtttc aagatgtaat taataccgac agtggtcttc tgatcaaacg
    14821 cttcaccggt tttaccatca aagagttgga ctttaccatc ttctggtaaa ccagccttct
    14881 taagctcttc ggaaatttgt tcaacggtca caccatcgaa tacaggtgta gctgcgtagt
    14941 agccaagttc agcagcagcc catccaaggt gggtttccat gagctgacca aggttcatac
    15001 gagagataac accaaggggg ttcaagatga tgtcaacagg acgaccatct gcaagatatg
    15061 gcatgtcttc ttcgggtaag ataaccgaga taacaccttt gttaccgtgt cggccagcta
    15121 gtttgtcccc gacggagagt ttacgcatct gggcaaccca gacttcaacc atcatgtaga
    15181 cggaagcggg aagttcgtca ccgttgtctt ttgagaagac tttgacacca acgactttac
    15241 cgcgttcacc gtgaggaagt cgtagactgg tgtcttttac atctttggct ttgtcaccga
    15301 agatagcgcg aagcaatttt tcttcggcag aaagttcggt ttctcctttt ggagagatac
    15361 ggccacacat aatgtcgccg gcagaaactt cggcaccgat acgaataaca ccgcggtcat
    15421 cgaggttagc gagcgcttct tctgagacgt ttggaatatc acgggttaag acttcaggcc
    15481 caagtttggt gtcacgtaat tcaagggagt acttttcaat gtgaattgag gtgtaacggt
    15541 catcacggac taagcggtcg gaaacgataa tggcgtcttc atagttgtaa ccaccgaagc
    15601 tcatgtaggc aacgaggaca ttctgaccaa gggccaattg accttgttcc gttgcgtatg
    15661 aatcagcaat aacttgacct ttcttaacct ggtcgccgac ttgaacgctc ggacgctggt
    15721 taatacaggt agcttggttc gaacgaacga agctttgtaa tttataaacg cgttcgccct
    15781 ttgtaccttc aacggtaatt gaagaagcgt caactttcgt caccgtaccg ttctcaacgg
    15841 cggttagaat ttgaccagcg ttcttaacag cgtcttccat accagtacca acacggggtg
    15901 tctgaggttt gataagaggg acggcttgct tttccatgtt cgcacccatg tttgcgcgag
    15961 cagcatcgtc atgttcgacg aatgggataa gagccgttgt catcgagaca gtttggcgag
    16021 ctgaaacgtc gatataatta acgcggagcg ctttttcgat atcaggttca ccaaatttac
    16081 gtacaataat agtttcgcca gcaatttcac catctttggt taaatcagtc gaagctggag
    16141 caataacatg gcgctcttct tcaccggcat caaggtatac aatttcgtcc atgacgcggg
    16201 gcttcttgcc ttctttttgc tcaacgcgaa gatacggtgt ttcgataaat ccatactcgt
    16261 tgacacgagc atacgaagca aggtagctca ccagtccaat gtttggacct tctggtgttt
    16321 caattggaca gatacgacca tagtgagacg ggtgcacgtc acgaacttca aatccggccc
    16381 gttcacgagc aagtccgccg ggaccagtcg ccgtgataca acgcatgtgc tcaatttcgg
    16441 caacagggtt ggtttggttc atgaactgtg aaagttgaga tgaagcaaag aattcctgaa
    16501 gtactgcagc gataggacga gagttaataa gctgtccagg tgtaatttct tcggcttcaa
    16561 gcaccgacat acggtcttta acgatacgtt caacacgcgc aaaaccgata cgcagacgag
    16621 cttggatcag ttcgcccacc gcgcggacac gacggttttt caggtgatca acgtcgtcgg
    16681 ccttcgcgtc aggggtattg tttaaggtga taatctcttt tactaccgca acaacatctt
    16741 caaggcgaag aacctggttt tcaggaacgt ttttgacgtc aacgttcaag cgttgattaa
    16801 gtttataacg gccaactttt cccaagctat aacgcttggg gttaaagaaa aggttttcta
    16861 ggaaagcctg agcggtttcg gctgtagcga gatcgcctgg acgaatacgc ttgtagactt
    16921 caatcatagc ttcttcacgg ctcttagttg tatctttatc cagagttgtc gtgatgtatt
    16981 tatgatcagc atctttatct acatcagcaa acgcttcgcg cactgcttcg ggttccatac
    17041 caaaagcccg caagaacgtt gttactggaa tcttacgttt acgatcgact tttacggcaa
    17101 taatgtcgcg attcgaggtt tcaaattcaa gccatgcacc acgtgatgga atgattttgg
    17161 cgccaaataa gttgcgtccg gcaacttctt cgttcgtgaa gagaacaccg tttgaacgaa
    17221 tgatctgcga aataacaacg cgttcaatac cattaataat aaatgtgccg cgtggtgtct
    17281 ggagcgggaa gtcgcctaag aatacgtcgg catctttggt tacaccggtt actttattct
    17341 taagaacaac atgtgccttt aggggagctt tgtatgtgag gttgcgagca actgcttcct
    17401 cctcggtata cttagcttcg tcgaggctgt aggaaacaaa ctcgagttca agtgcttttc
    17461 ccgtaaagtc agttactggg ttgatctcct caaaaagctc cttgattcct tcgttaaaca
    17521 gccactgcca ggactgtgtt tgaagttcta caaggttagg gagaggaacg gtgactggga
    17581 acgacttaaa atactttcgt tcaatcatgg cggctccttg gtttggtagg aagtaacctt
    17641 ttcatttcgc agaaattcgg tgtgacgatt tcgagggaac ttttatcttc tctctactac
    17701 ggtttcagag acacgtccga ctgcaagccg cgcatgagcc acgcatactc ttatacgagc
    17761 gccctacgca cctttcgttc ggatgtatct ccatgcaacc ttaactggcc agatagatta
    17821 aaagttctca gacaacacaa gaatccccaa attattgggg actaacaaca aaatacctca
    17881 gctgatagtg actgattcga gtatcgtaac ctccgaagct tctagataca ataatagtga
    17941 tttttgctaa tgttgtcaag tcttaacata gcatggcgct agagctttat ctaaagccag
    18001 tggcgattgg ctattcttca gacagttgct cgcctatttc tgctaaaagc tctttaatat
    18061 tctcacggga aaccgcagaa atggtgtgac gagagaaaag atgtgacgat tgagaaaggc
    18121 aggcttttag tgcttctatt ttcacttgtg tttcttcggc aatgtcatca gtaattaact
    18181 ctgatttggt aataacaaca atttctgatt tttgagcaag tccactatcg aatttctcaa
    18241 gttcattacg cacagtcgag tagtctcgcg catagtcatc cgataagctc tcgatgaagt
    18301 gcactaatgt cttggtgcgc ttcacatgtc gtaggaattg atggccaagt ccttttcctt
    18361 ctgacgcacc ttcaatcaac ccagggatat ccgcaaaaac gaaccgtttt tccctatagg
    18421 gtaccactcc tagcacagga ctgagagtag taaaaggata attgccaatc ttaggttgtg
    18481 catcgctgac aattgaaagg aaggttgatt taccggcatt tggcaaacca atgattccta
    18541 cgtccgcaat aagttggagt tcaaatttaa aaacagctcg ctcggccggt tctcctggct
    18601 gagcataccg gggcgtttga tgagttgccg tcgcgaaatg aacatttcca agtcccccat
    18661 ttccgccctt taagacccgc ttgcgcgaat ctgcttcagt catatccgcc acgagccgcc
    18721 atgcggttcc ggcctgacga taaactcggg ttccacgtgg aacaaatacg gttaagtctt
    18781 cccctttgcg gccaaagagt tttttacctg cgcctggtac gccgctctcg gctttgagtt
    18841 cacgcactga ggctaagtgg tttaagtcgg tcagctgtgt attgacttca ataaaaacac
    18901 ttcctccccg accaccgtcg ccaccgtcgg ggccgccttt ggggatgtat ttttcacgtc
    18961 gaaacgagac ggctccgtcg ccgccttttc ctgattcgag acttaaagac acctcatcgt
    19021 gaaacatagt tctacaagta taccttgaaa cgagagatta agaaaggtaa acgtatacaa
    19081 aatacttata aattcttaaa aagactagaa aggacttact gcccggccac caacgcgtag
    19141 ttcgaaatgc aaatgggggc cggttgagcg gccagtatta cctgagtaac cgataagttc
    19201 gccctgagac acccgttgcc cactgcggac actgacatca ctcatatgtc catatcgggt
    19261 agttttgtta cccgtgtggg aaatgagaac tgttttgcca tatccgccat tccaccctgt
    19321 ttcagtggca acgatcacta ctccatcggc ggccgcaacg ataggtgtcc ccacattcgc
    19381 ccgataatca attcctgtgt gaacccctgc aatcaagcct cgcgagattc cattatggtg
    19441 aatcggaacg attaaaccat atccaccact cgcttttgca gtggaaattt ttgctacgct
    19501 tttctttgcg tcagcgagct gctggctcgc tttttgtcgg tcctctgccg ccttaattgc
    19561 cttgtcggac aaatcatcaa cgggaatcgt gagttcttgc cctggcttta aggtatcaac
    19621 attattaatg ttatttttaa ccttaatagt tgctactttt aacccaaatt tcgagccgat
    19681 cccgctcatg gtatctccaa tctggacggt gtatttaatt tctcgtgtac gatcggctgt
    19741 acttgcaggc ggagctggcg tttctgtcgc atttggcgta tcggactcac ttggcaacgt
    19801 tacaataaga gggttagtag aaatgaagtt tccattaacc cgttcctcca gcgccagggc
    19861 aattgcgtcc ggatcttcgt cgacgtcggg agtgtagtca gccagaagcc gtactgtatc
    19921 agcgataaca tatgggtcgc taactaggac gtccgccggt atggacggaa catcgttagc
    19981 ggcccgaacg atcaacgtat tgtttactaa aataaaaatc gtcagaacca caagcgcctg
    20041 atgaatacga gagtattcgc gcacggcgtg gtacgtctgc gtgccgtaat gacgtgtctg
    20101 ggtaatagac ttatgttgtg tgagctgttt tagaaaagca aggacctggc ggtataacaa
    20161 tccgattcgt tgctgcatgt acactaaagt aacgtaatca ggctaaatgt caacgaaaat
    20221 ttggatattc atctaagttt cacgagtgag aatacgtgag ttccgtataa atacacaatg
    20281 ctcccgctcg tgatgagggg agcattgtgt atttattgtg gaagttattc ggttggagcg
    20341 gtatcgttaa cgatcacttc ttcttcgctg aagaagataa ccatatctcc ctctttcacc
    20401 ttctccgata cagaaactga aacgccacat tgctcagggg cttgaagctc atctaccgcc
    20461 tcaggccctt tttgaagcgc aagaactttg cctttgccaa gttcctcgcc agctcgcatt
    20521 aagctaattt gggctttttt catggcaata ccctgcgtga tctctccgcc aataatttgg
    20581 gcttcttttg tcgttcggaa gactttaagc accttcagac gaccacgttc aaccaaaatc
    20641 ttttctgtct tcacgagacc tttgagtgcc agctcgatga ggttaagcat gtcataaata
    20701 atcttaaagg aggtaacagt aacgcctaaa ttctctgccg ctttttttac ttggggagaa
    20761 accgtaacgt taaagccata gacaa
//
LOCUS       DASWYB010000048         4787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_283233, whole genome shotgun sequence.
ACCESSION   DASWYB010000048 DASWYB010000000
VERSION     DASWYB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283233"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGE59_02805"
     CDS             complement(<1..187)
                     /locus_tag="VGE59_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074608.1"
                     /translation="MERNDDRLLFLEELAEYLDIPTVVTSLESLEFLPGHQEEHYLVR
                     DILHDVARKVMVSIVEVL"
     gene            332..1096
                     /locus_tag="VGE59_02810"
     CDS             332..1096
                     /locus_tag="VGE59_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074609.1"
                     /translation="MNKSNTPHSIEDFLTELGLTPKQSKVYLSALQLGPASVQAIANL
                     AKIERTNVYDSIESLIAKRLMSITATGKRTLYVAEPPETLQKILEEKQATLKQLLPDL
                     RSLTHSSDAKPRIRYYDGIEGYKAVYEDNLTCKDKLLFGIYSVKTSVEVLGRDYLRLA
                     IERRVKAGIRLRVIRSRQTEVEGIYRSSEEELREVRFAPEDMVFPITTFVYDDKIVFL
                     SSEKELFGMIIESKDMASAYRNCFQALWQISTPPTF"
     gene            complement(1093..1869)
                     /gene="uppP"
                     /locus_tag="VGE59_02815"
     CDS             complement(1093..1869)
                     /gene="uppP"
                     /locus_tag="VGE59_02815"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEY1074610.1"
                     /translation="MFHAIILGLIQGLTEFLPISSSGHLILIPEILGWMPSGLTFDVA
                     LNTGTFLAVLIYFFPTWWRLLVKGVLGGEKKEQKILGYLVLATIPAALLGYLLEPVID
                     GSLRQPLVAALMLVVFALVLYWAEKQAALKREIQDLTWGDALKVGLAQALALIPGVSR
                     SGITMTAGLSLGLKKEEAAEFSFLLLAPISFGAALLQAPDVLKATNTSEMALGALVSF
                     VVGILAIHVLMRFIKQYGFAPYVWYRVVVGILALAFLIFS"
     gene            complement(1879..2457)
                     /locus_tag="VGE59_02820"
     CDS             complement(1879..2457)
                     /locus_tag="VGE59_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY1074611.1"
                     /translation="MSSLLVVDESIVRFLNGLLVSNTALGAMVFTISLYAVYVIPFVW
                     LGWWFLASKKTRYDLFSSMFAGLVAWEVINRIAKLFFERQRPIHELPLKEILFERPEN
                     SFPSDHAAFMSAIAFFFLLRGQKKAASWLFVLALAAGIARVAVAVHYPSDILVGFLDG
                     FLAAWVVSYFHEVLSKLIWTPLLRLAQKLHLA"
     gene            2549..3559
                     /gene="xerA"
                     /locus_tag="VGE59_02825"
     CDS             2549..3559
                     /gene="xerA"
                     /locus_tag="VGE59_02825"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEY1074612.1"
                     /translation="MTIEQTIRRFLEHLEVEEGKSRLTLRNYEHYLATFLHFCDDEKI
                     KEPNQITQNLVTLFRLWLNRPHKQVGKNDYLERSRKTQNYYLIALRSYLKYLSSRDIV
                     SLPPEKITLAKTESAQITFLEPEEIERIIQQPEVAEFQGKRDRAILEMLFSTGLRISE
                     LTNLNRDDLQLETGEFSLRGKGGKVRVAFLSDDCIKYIKSYLKHRVDEDKALFIRTQS
                     NQEDTHEVNPDLRLTPRSVQRLVQKYTTQAGIAKKVTPHVFRHSFATDLLQNGADLRS
                     VQELLGHSSVTTTQIYTHITNPQLQEVHKAFHARRRNQTAPEEKSALHSENSGQSDES
                     NA"
     gene            3656..4087
                     /locus_tag="VGE59_02830"
     CDS             3656..4087
                     /locus_tag="VGE59_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074613.1"
                     /translation="MRGVLAFLLLMSALLAGTPARAHERKSSSLSFGLGFSYSQRKES
                     YSRPRRDYDRRYAPPYQRPYVDPGPPLSYYGRDYDPYYDPVPQPRYIPERGYSEMYYQ
                     SQFGYSYGGFSPGYAAPLYGGYTGYSRCPCCGMPLLPTPYY"
     gene            complement(4162..4605)
                     /locus_tag="VGE59_02835"
     CDS             complement(4162..4605)
                     /locus_tag="VGE59_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1074614.1"
                     /translation="MSSPRIHKVAIVEASPERVFDFLVDPEGHSGILPGLIEVTDVPK
                     PLRVGSDFHFKFQMYKITLSGEWSVTKLEYPHVYEGRSSGGAVSFWRYELKPVQLGTQ
                     LELSIEYTPPQTVLSSISQPVLESLNEQSAQQFLDNIGLILKQKF"
BASE COUNT         1456 a         1253 c         1004 g         1074 t
ORIGIN      
        1 ccagaacctc tacgatcgaa accattactt ttcgagccac atcatgaaga atatctcgaa
       61 ccagataatg ttcctcttga tgtccaggca gaaactcgag cgattccaga ctagtcacaa
      121 ccgtggggat atcaaggtac tcagcaagct cctcaaggaa gagtaaccga tcatcattcc
      181 gttccatttt ttcaccctcc atagaccgat cactttctgt gccagactat acctctagtc
      241 gtgcattttg taaatacaga ttatacaaat atttgtataa ttcgccacaa cacttgacga
      301 ataacaggta caggacatac aatatatatg tatgaataaa agtaacactc cccattcgat
      361 tgaagatttt ttgacagaat taggcctcac acccaagcaa tccaaagtgt atctttcagc
      421 tcttcagctg ggtccggcct cggtacaagc aattgcaaac ctcgccaaaa tcgagcgtac
      481 taacgtgtat gactctattg aatcccttat cgccaaacgg ttgatgagta ttacagccac
      541 cggaaagcga acactctatg ttgcagagcc acccgaaact cttcaaaaaa tcctcgaaga
      601 gaaacaagcc acgttaaagc agttattacc cgacttacga tctctcaccc actcaagcga
      661 tgcaaagcca cggatccgtt actatgacgg tatcgaagga tacaaagctg tttacgaaga
      721 caacttaacc tgcaaggata aacttctgtt tggtatctac tcagttaaaa cttcagtcga
      781 ggtactggga cgagactatt tacgcttagc aatcgagcgg cgagttaaag ctggtatccg
      841 acttcgagtt atccgatcta gacagactga agtagaaggc atctatcgat cgagtgaaga
      901 agagctacgc gaggttcggt tcgcaccaga agatatggtt ttcccaatta ccacttttgt
      961 gtacgacgat aaaattgtct ttctctcgtc cgagaaagaa ctttttggga tgattatcga
     1021 aagtaaagac atggctagcg cctatcgcaa ctgctttcag gccctctggc aaatcagcac
     1081 cccgcccacg ttttaactga aaatcaaaaa ggcaagcgcc aaaataccca ccaccacgcg
     1141 gtaccagacg tatggagcga agccatattg ctttataaag cgcatgagaa catgaatcgc
     1201 aagaataccc acaacgaagg aaacgagcgc tcctagggcc atttctgagg tattcgtggc
     1261 cttcaaaacg tcgggggctt gcaagagtgc ggcaccaaac gagatcggcg cgagcaaaag
     1321 gaacgaaaac tcagctgctt cttctttttt taacccaaga gaaaggccgg ctgtcatggt
     1381 aataccactc ctcgataccc ctggaataag ggctaaggct tgagcgagac caacctttag
     1441 agcatcaccc caggtaagat cttgaatttc tcgtttgagc gcagcctgtt tttccgccca
     1501 ataaagcacg agcgcaaaaa ctacaagcat gagagcagcc actagcggct ggcgcaaaga
     1561 tccgtcaata acaggttcga gaagatagcc aagtagcgct gccggaatag tagccaacac
     1621 caagtaaccg agaatttttt gctctttttt ttcgcccccc aaaacacctt taaccaaaag
     1681 tctccaccag gttgggaaga aataaattaa gacagctaaa aaagtaccgg tattgagagc
     1741 cacatcaaag gttaaaccag atggcatcca tcctagtatc tccggtatta agataaggtg
     1801 accggacgaa gagataggca aaaactctgt caaaccttgg atcaaaccga gaataattgc
     1861 atgaaacacg ggcaactcct atgctaagtg taatttttgt gccaaacgaa gcagtggggt
     1921 ccagataagc ttactcagaa cctcatgaaa ataactcacg acccacgccg caagaaaacc
     1981 gtcgagaaaa ccaaccaaga tatcgctcgg ataatgcacg gctaccgcaa cccgagcaat
     2041 tccagcggca agagctaaca caaagagcca gctcgctgcc ttcttttgcc ctcgcaaaag
     2101 aaagaagaag gcaattgccg acataaatgc ggcgtgatca gacggaaaag agttttccgg
     2161 gcgttcaaag agaatttcct tgagcggtaa ttcgtgaatt ggtcgctgac gctcgaaaaa
     2221 gagcttcgca atccggttaa taacctccca cgcgacaagc ccggcaaaca tggaggagaa
     2281 caaatcatat cgagtttttt tactggctaa aaaccaccac ccaagccata caaaaggtat
     2341 tacatagacc gcatacaaac taatagtaaa caccatcgca cccaaggcag tattcgaaac
     2401 cagcaaccca ttcaggaaac gcacaattga ttcatcgaca acaagaagag aactcatacg
     2461 attcagtata aagcgagaac tctaaaagcg aaagcgcttt gacttttctg acaagcgtcg
     2521 cataatataa aggttggagg gagccgaaat gacgatcgaa cagacaatcc gccgtttttt
     2581 agaacatctt gaggtagaag aaggcaaaag tcgcctgacc ttgcgtaact atgagcacta
     2641 tcttgcgact tttttacact tctgtgacga cgaaaagatt aaagagccaa accaaatcac
     2701 acaaaatctt gttaccctct ttcgactctg gctcaatcgt ccccataaac aagtgggtaa
     2761 aaacgactac ctcgaacgga gccgaaaaac ccagaactac tacctcatcg ccttacgctc
     2821 gtacttgaag tatctctcga gccgggatat cgtttcactt cctcccgaga agataaccct
     2881 cgcaaaaacc gagtccgctc aaattacttt tttagaaccc gaagaaatag agcgaatcat
     2941 tcagcaacct gaggtggccg aatttcaagg caaacgcgac cgggctattt tagaaatgct
     3001 tttttcgacc gggttgcgaa ttagcgaact aaccaactta aaccgagacg acttacagct
     3061 cgaaacaggg gagtttagct tacggggcaa aggtggaaaa gtccgggtcg catttctctc
     3121 cgacgactgc atcaaataca ttaaaagcta cctaaagcat agggtagacg aagacaaagc
     3181 attatttatt cgtacccaga gcaatcagga agatacgcat gaagtaaatc ctgatttacg
     3241 ccttacccct cggtctgtcc aacgactcgt acaaaaatac accacccaag caggtattgc
     3301 caaaaaagtg acccctcacg tctttcgcca cagctttgca actgatttgc tccagaacgg
     3361 ggcggacttg cgctctgtac aagagctgtt agggcatagc tcagtcacga cgacccagat
     3421 ttacacccac atcacaaacc cgcagctcca agaagtccat aaagcctttc atgctcgccg
     3481 gcgaaaccaa accgctccag aagaaaaatc tgcactgcat agcgaaaatt caggacaatc
     3541 agacgaaagc aacgcctaac gcactgctct tttagacaat cacgcattgc tcttttaccc
     3601 tgaaactggt atacatcact ataacgagac cgcataccga aaggagcaaa aagcaatgcg
     3661 tggggttctc gcgttcctcc tgctgatgag cgctctgctc gccggcaccc ctgctcgagc
     3721 gcatgagcgc aagagcagct cgctctcctt tggcctgggc ttttcttaca gccagcgcaa
     3781 agagagctac tcccgtcccc gacgggacta cgaccgccgc tacgccccgc cctaccagag
     3841 gccgtacgtt gacccgggtc cgcctctgtc gtactacggc cgggactacg acccctacta
     3901 cgacccagtt ccccagccgc ggtatatccc ggagcggggc tactccgaga tgtattacca
     3961 atcccaattc ggctacagct acggaggctt ttctccgggt tacgccgcac ccctgtacgg
     4021 aggctacacg ggttattccc gctgtccgtg ttgcgggatg cctctcctcc ccacccccta
     4081 ctactaggcc gcgacctaga acaacgccgg gactccttct ctaaccaaga ggagtcccgg
     4141 cgttcctcat tttttacatt attaaaattt ttgcttgaga attaaaccaa tattatctaa
     4201 aaactgctgt gccgactgtt cattaagcga ctctaagact ggctgactga ttgaacttaa
     4261 aaccgtttgc ggtggcgtgt attcaatcga gagttcaagt tgcgtaccaa gctgcaccgg
     4321 ctttaactcg tagcgccaaa agctcacggc cccaccagaa gaacgccctt cgtaaacgtg
     4381 tgggtattca agctttgtca cagaccattc accggaaagg gtgattttgt acatttgaaa
     4441 tttaaaatga aagtcagaac caacccgaag cggtttgggc acatcggtca cctctatgag
     4501 cccaggcaaa atccctgagt gcccttccgg atcaaccaga aagtcaaaca cccgctctgg
     4561 tgaggcttcg acaattgcta ccttatgaat ccgagggcta gacatacttc ctccaagaag
     4621 aattattctt attttggttg attcccagga ataggctcac ctaaatccca gagaatggaa
     4681 aataaatttt caaagacacg aactaagctg gagtttttaa cctcaataat cattggctca
     4741 gtggctgagt gcaagataac gctttctgat ccaaaaatat taaagtt
//
LOCUS       DASWYB010000049        15445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_593932, whole genome shotgun sequence.
ACCESSION   DASWYB010000049 DASWYB010000000
VERSION     DASWYB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593932"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VGE59_02840"
     CDS             <1..387
                     /locus_tag="VGE59_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074615.1"
                     /translation="FQTYWENHLNRMTQGDTYYVLGSLGDLWNELLQEKATRMKRLLV
                     KKEIRVKVIAYNDNAKDRELTALYPYYDVRIMPNGYYTPANANIWGDHIALQTAVEPY
                     SVIDIYNPALAKAYLNTFNVLWESAK"
     gene            complement(438..1301)
                     /locus_tag="VGE59_02845"
     CDS             complement(438..1301)
                     /locus_tag="VGE59_02845"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074616.1"
                     /translation="MVSSVAMPVLGGSKYELWVVDALVDYRRQSDWLEIAVVKGYALV
                     RVQMPTSGGKSLQEAAYEAYEAVFAQAKTRKMSLLRAWHWIPRILEEVGGLENYRWHN
                     VGRQEAWKDFGPWQDGESVLPAATGIGVQEGPYVLEVLLTKYPVIPLQNPRQVPFHRY
                     SSKFGPKPPFCSRGALHLAPEGPEVWISGTASIRNQYSWWKDNPREQVKETFRNIRAL
                     IGDANLEAHGVKGFSLENLAHHRAYVKRPEHYAVISEELNLLVGDGSRCDLQTDVCRQ
                     ELEFEFEAIAR"
     gene            complement(1498..2247)
                     /locus_tag="VGE59_02850"
     CDS             complement(1498..2247)
                     /locus_tag="VGE59_02850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1074617.1"
                     /translation="MLIEKFGISVAQFLGSGRLIEEADAEPILGLAADGFEADAELHL
                     ERYRPLARFVARRMEKRRIARPYVVEMGPGPGIWAAEAAKLMPGAVIDCFDLSPDMVE
                     KANRRFKVQGFSHRVAAYYQDMRGVHQETGAKADMVFSRNMLHRLPDLYQALLAMLYA
                     ANNQGEVFVTCFLRVAQQDSAGQRRFWENVASKADFPELQLAYVQAHVFAHSLEEYRQ
                     AAQAVARVASARFQIWTGGNNEVYLHFLKGG"
     gene            2500..3285
                     /locus_tag="VGE59_02855"
     CDS             2500..3285
                     /locus_tag="VGE59_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074618.1"
                     /translation="MTPQLYSVLHDIGLSEKEAKVYLAMLELGESSIIPIARHAGIKR
                     TTVYNYLEDFTRLGLISITIRNGRRYYVANSPNRLRTMLRSRLEQVETIIPNLFSMWK
                     EEEEKPSVQMFEGIDGLKRVFELSLECESKKIDVIPIESAGHVFVGPEYISRYIEKSR
                     EANITFRSLRVPSDKYENYRHFEPEEHDNRTIKVAPDWFTPQSYIQIYDNNVGIFSHA
                     KEIPYALVITSQSYAHTQRLFFESIWQQSTPIREWLREKEKTE"
     gene            complement(3350..4429)
                     /locus_tag="VGE59_02860"
     CDS             complement(3350..4429)
                     /locus_tag="VGE59_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074619.1"
                     /translation="MNPELDPQLNAFKGEYWRTMGKVSAGLNIFATQYACAKSDSATR
                     RYTQKRCHLIGLKFYAGYLAGGGTEVRWEDHQLLAIALELVMTSAYAANQILDHKKAV
                     WDAPGGIEQALLDYLFLQTWQSDLIERWTSLSQLDEQYTKRVKWLMSQLAEKMTLGFG
                     VERELSVRHRPLNQMTSSRFWWAQRYQERNTSLNLVYDWAPLIGHDIARTEVRGYEER
                     LDTVGHHYDFFGNCIPFSHSMQVINDIADFLPNACDLGVKVYQDRFADVRNGLVTYPV
                     HELLDVPQMVEVLRDGDVENSGWQDYMIKLVKERGIMDKAKEQCERSWYHHLTFWKWI
                     LRRHDLLLLSTYSMLRHNKYFKSIE"
     gene            4620..5378
                     /locus_tag="VGE59_02865"
     CDS             4620..5378
                     /locus_tag="VGE59_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074620.1"
                     /translation="MMQHLTSILNQFGLADKESALYLASLTLGESGMSELAQKAKLKR
                     TSAYVVFKALEQKGLMGSFKMRSGTKFVATPPEVVIRKTEQQLMQLQAVLPEFQAIQD
                     KANGPKITYFEGEEGYRVAAEDSLKFAGETIRHIGSLEEIHKVISRDYDINYYIPKRI
                     HNRIRLKALYFKSDFNETNFMRDDGAELRQIRFLPESFKYPTSLLIYGNKVAFFSTAK
                     ELITVIIESADLVESERKKFDLMWELLDNKETGV"
     gene            complement(5563..5639)
                     /locus_tag="VGE59_02870"
     tRNA            complement(5563..5639)
                     /locus_tag="VGE59_02870"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5603..5605),aa:Glu,seq:ctc)
     gene            complement(5713..6333)
                     /locus_tag="VGE59_02875"
     CDS             complement(5713..6333)
                     /locus_tag="VGE59_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY1074621.1"
                     /translation="MTTALRLITAPSRAIRKLYNWTIHWSKTKHASRALFVIAFAEAS
                     FFPIPPDVLLIPMVISDRARWWRTALICTVGSVLGAMLGYAIGMGLYKSVGQPIVDAY
                     GLQSTVNLVGQKYADNAFLTVLTAAFTPIPYKVITIAAGLFKISFPTLVFASVVGRAG
                     RFFIVAGLLRVFGEKIEEKIEKYFDLFALLFFALLVGGFFAVRYLH"
     gene            6558..7337
                     /locus_tag="VGE59_02880"
     CDS             6558..7337
                     /locus_tag="VGE59_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074622.1"
                     /translation="MKLVFWTLVCSLALCFPHGSWANPPMVRGYTLPSHRQQPHPRAS
                     SARKTNDEPQVRHYNLTAPKPRPITKTKPYVAPRKEEKKIVVREYDLRDVNPVDPTHP
                     LKGLVPEVKVSELEKHVGEATKIITYKLNQNKDFATAKSTLSESWGAIKERVRQVVRE
                     ELKKHPTRTTTKVIRETRVVQGPAAAPAAAPRIDEAALEARITNNVLAKMQNQAKSKP
                     AQPAPAEERLKNLEAMAYGLVAVVFVLFLGFLYLHKTKEDK"
     gene            7338..8474
                     /locus_tag="VGE59_02885"
     CDS             7338..8474
                     /locus_tag="VGE59_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074623.1"
                     /translation="MRTLLLLSGIAFVVLVIGGTIISDVLKRRRNAGLPRRRVLDPPV
                     TPPEDISYACTVRLPDKREFTVRNKAGVIFTPNVPTEWEGNPFSHLHSEHEGAINNFS
                     PEKVTQPAADIVVTAHYKEKKKPGPFEEVVVPKAPPRPSIPRHVDVIRAVNDLDIPPF
                     LLKDPVRLEVKNGEFVYRDQFSQVAELRPGNTLVARAYPGLAGSTFSHWEVEGDMTLP
                     PREEHVSPMSLTVQGNATLTAVWKELETPPAEEVPEAPQEPPPSPIEQVIEEMLPEQG
                     ISLEPPGETTPAEASAPEVSPESTEEEEPTQQPESGPQEEEPAAPPESAPVEAPVEEV
                     TTAEEDDQPVAEEEAAPVTNPDAPASGKPGVRKLPAGAGIRKKQ"
     gene            8616..10634
                     /locus_tag="VGE59_02890"
     CDS             8616..10634
                     /locus_tag="VGE59_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074624.1"
                     /translation="MEQEPNHDPRQKLPDWEAEGPDPEEVREAEEKQREANFEHEKKV
                     FETTHYLNHVVETQFDGRTYEDLSSEDKLYAITLVNRQHNTHRVNTLVAERNSGVQGL
                     KHKLDDKLGRSKIWGFAKNIALGAGGSLAMRTMTAGLVATGGFVGGAISGAGAGIVTG
                     SYRGWSNFNKEQLNFRALENEIGANELITKGLHESPQEIEQEYGSLEAYYSKVHQALA
                     TYREIEKSNLLSDNREYVSRFYENLASLESISQIQGIKQQAESLGRDPTPEEAADLLC
                     KALNQRELANVERFDKGLAQKAQEFVRTRKSERLKTTVKQGLIGGATGAVLGGTVGWL
                     LELVFHSPTAAPTGEKINIPNIAGGGHTDPSLAQDYAIHNKQFLDVTEAIRNGNTQEA
                     ITQGWQIHLNLDTDPSVVTRLHEIAQQSNLSPDEYNRLAYNVLTYANREGIDNNALLT
                     YAQQAITDPHALQSLSPQAQQSMQEIFGTTHLTRDSFINAHEFRNLSTIDTSTDAYYH
                     HLKEAGALAQAGIDRTATLVTGSSAAATVPKIVSKFSPPKEKINPEPHVEEEEEEEEV
                     NDEAYINQELTFDNFENFRIAEVSVLSTEKEIRATLPISSGENANVVLHGDITDPDTL
                     RELTALIDQIESGANKPTLTAGYTFVVSRAERDASNTLSLYATLKKTY"
     gene            10644..11003
                     /locus_tag="VGE59_02895"
     CDS             10644..11003
                     /locus_tag="VGE59_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074625.1"
                     /translation="MDTHPHPSTPFPTDQAAREVLKRLNMEEISDEAKERLLTQIADL
                     IDQEILYQVMSRLSNEDITELGEFLQTDENSHEESLKPEAFNFIASKIPHLDVIIRDS
                     IVKVYEDIVSTKNLLDA"
     gene            11042..11578
                     /locus_tag="VGE59_02900"
     CDS             11042..11578
                     /locus_tag="VGE59_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY1074626.1"
                     /translation="MQEPGIVVVLDNIRSVYNVGSIFRTSDALGVAKVYLCGITPTPD
                     HPRMSPSQERDSFHNEPDKYGRTFSVAKTALAGLHSVAWEHTPSTLETVTHLKKSGYT
                     VIAVEITDTSQPIHPISSRPLAIVLGNEKEGINQEILALADEILHIPMHGVGKSLNVS
                     VSYGIAGYELLRQSSNPQ"
     gene            11625..12410
                     /locus_tag="VGE59_02905"
     CDS             11625..12410
                     /locus_tag="VGE59_02905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1074627.1"
                     /translation="MGEKAIKWPASITLMRHAVSAYNDLKRQWVGDPDYERFVTLYNT
                     DFHNPETLRLARVIKSRYALGCSDADTPATKAGLEDALITGSRLHEVMELPTTIFVSP
                     YKRTKQTLAAVIKGWPALGNVRIVEDPRLREQEHGLSTVYNNWMVFHAHHPEQKELYD
                     LDGSFRYRYPQGENGYDVQDRGRDIFGTWIRDYRGERLFVVTHHLTILTVATLISRGG
                     EQEFKDLDKHHKPVNCGLTQYVCDPDQGKEGKLIQRYYNRKLY"
     gene            complement(12481..14178)
                     /locus_tag="VGE59_02910"
     CDS             complement(12481..14178)
                     /locus_tag="VGE59_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY1074628.1"
                     /translation="MIQVKLQTIAKQLNKTITDIAHDTGLNRNTITALYHNQVDGIKF
                     GTIEQICKTYRLKLNDLIEMQIDAERRSDFDLPPKLYKQEGSIVPFTCWPWVMAITQP
                     DQAVFSDHYSILYAYFQRDYGMGYWDYYNMQRVSQGVYRRYNSPKDLELLFQIFLPYA
                     RSLEAAYRNLDIEALLQMSASQVRQFSDGIWDQYRQFWVYSLFIDSFDAGFDQEQIQI
                     IAKKYDLTIDEIVVLTTPQELAFTNERYIELLKIAKRLSRHKSLGMPTVTAVKEVIAG
                     DEGVAEYIKAFDYYKSNYAHISHITLEETAEELTKYLLDKDLLTAELHRLEQYSAQQI
                     KKQKAVLRKHRLTHNPLEFFAKLTFWREYRKQVNLKGIHVLDGLLQALQKQTGIPYRY
                     LQYLSFEEVDAVLKGLVSLDVLKKRCEQGGLVIIQGNSYKMIEGDEASSLRDDLEEML
                     NEANEDPVVIPGNIASQGYARGVAKVILDRAEFDKLEEGEILVTGMTRPEFVPVMKKA
                     AGIVTNEGGITCHAAIVSRELGKPCIIGTKNATQLIHDGDLIEVRANHGSVRILKRA"
     gene            complement(14259..14882)
                     /locus_tag="VGE59_02915"
     CDS             complement(14259..14882)
                     /locus_tag="VGE59_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074629.1"
                     /translation="MDRRVPRVIGVRHAQYDRATGLHNEEGKATLAQTVGRINEIIAD
                     DPPASIEIWWSPTFRTNEVAAQLVSTFSSRVDRIVMANELGCEDIIVANETEFPVSEA
                     KARIAARIEAGETLTAGVYLEEYPPARQVQTRVLNFLMDQVLNPQDREVEPNRLVILV
                     SHEGVIDLLADPGFPCLGYGEMMHFEVEYNSDFGPEWVYVPALTRRH"
     gene            complement(15063..15136)
                     /locus_tag="VGE59_02920"
     tRNA            complement(15063..15136)
                     /locus_tag="VGE59_02920"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15102..15104),aa:Gln,seq:ctg)
     gene            complement(15122..>15445)
                     /locus_tag="VGE59_02925"
     CDS             complement(15122..>15445)
                     /locus_tag="VGE59_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074630.1"
                     /translation="WNRTYYENNRLLERGRILERKKSIAEKLAFLKSSKSCCVCGESS
                     SVCLDFHHLDDKTKSFNLSDVKSWGWSMKKVEAEIEKCVVLCANCHRKVHAGIIDITN
                     HSGIV"
BASE COUNT         4278 a         4154 c         3500 g         3513 t
ORIGIN      
        1 tttcaaactt attgggaaaa tcacctcaac cggatgaccc aaggcgacac atactacgtc
       61 cttggctcac ttggcgacct gtggaatgaa ttactgcaag agaaagccac tcgaatgaag
      121 cgtcttttgg ttaaaaaaga aatccgcgtg aaagttattg cttataacga taatgccaaa
      181 gaccgagaac tcaccgccct ctacccctat tacgacgtgc gaatcatgcc caacgggtac
      241 tatacccctg caaacgccaa tatctggggt gatcacattg ccttacaaac cgccgttgag
      301 ccatattccg taatcgatat ctataacccc gccctcgcca aagcctatct caataccttt
      361 aacgttttat gggaaagcgc aaaataataa aaagagcccc gcctattaca gacggggcac
      421 atgacacaga tacgacgcta tcgcgcaatc gcttcgaact caaactctag ttcttggcgg
      481 cagacgtctg tctgcaggtc gcaccgagaa ccatcaccca ccaaaaggtt cagttcctcg
      541 ctaatcactg cgtaatgttc gggccgcttg acgtaagcac gatggtgggc gaggttctcg
      601 agcgagaaac ctttaacacc atgtgcctcc aggttcgcat ccccgatcaa agcgcgaata
      661 ttccggaagg tttctttgac ctgctcccta gggttatcct tccaccagga gtactggttc
      721 cggatggagg ccgtacccga gatccaaact tcgggtcctt cgggagccag atggagcgca
      781 ccccgagaac agaagggcgg cttgggccca aacttactcg agtagcggtg gaatggtacc
      841 tggcgcgggt tttggagcgg gataaccgga tacttggtca gaagcacctc gagaacatac
      901 ggaccctcct gcaccccgat ccccgttgcc gccggcagga ctgattcgcc atcctgccaa
      961 ggaccaaagt ccttccaggc ctcctggcgc cccacgttgt gccagcgata attctccaac
     1021 ccacctacct cttcaagaat ccgcggaatc cagtgccagg cccgcaagag actcattttg
     1081 cgcgtcttcg cctgagcaaa aacagcctcg tacgcctcat acgctgcctc ttggagcgat
     1141 tttccacccg aagtgggcat ttgcacacga acgagcgcat accccttcac cacagcaatc
     1201 tcgagccaat cgctttgccg gcgatagtcc acgagagcat cgactaccca taactcgtac
     1261 tttgatcctc ccaggaccgg cattgcgacc gagctaacca tataatcctc ttcaacctcc
     1321 tcgtaacgat ctccaaatca cacttctcgc agaatccgtc ctgcatactt cagccgagcc
     1381 gcttcaatct cgttctctcc tagcgtcaga accgtaatca ttgccttttc tccatatctc
     1441 caagcaacca cgattgattg cctcaagtat ggccccgacc gaagtcgggg ctaagaccta
     1501 cccgcccttg agaaagtgca ggtagacctc attattgcct ccggtccaga tttggaaccg
     1561 ggcgctcgcg acgcgcgcca ctgcctgcgc cgcctgacga tactcttcca ggctatgcgc
     1621 aaacacgtgc gcctggacgt atgccagttg cagctcgggg aaatcggcct tcgaagctac
     1681 attttcccag aaccttcgct gcccggctga atcctgttgc gccacgcgta agaagcaggt
     1741 cacaaacacc tctccctggt tattggccgc gtacagcatt gccaggagcg cctgatacag
     1801 atcaggcaag cgatggagca tattccgact aaataccatg tcggccttcg ctcccgtctc
     1861 ctgatgtacg ccgcgcatat cctggtagta cgctgccacc ctgtgagaga aaccctggac
     1921 cttgaagcgg cgattcgctt tctcaaccat atccggagac aggtcaaagc agtcaatcac
     1981 cgctcccggc atcagctttg ccgcctccgc cgcccagata cccggacccg gccccatctc
     2041 tacaacgtag ggccgggcaa tgcgccgctt ttccattctc cgagccacaa accgagccag
     2101 aggccggtag cgctcgagat gcagctcagc gtctgcttcg aacccatccg ccgcgaggcc
     2161 caggatcggc tccgcatctg cctcttcgat aagccgtccc gagccaagga actgcgccac
     2221 agagatccca aacttttcaa tcaacataac ctttatctcc gcggacgagc cgcatacatg
     2281 aaggacgatt cctccaactc aacggataga agctgccgca cttataccca ctgagttaga
     2341 tgcggttgtt aaattgcttg ctgtcatcgt aacgagcagc atcataccta aaaaagtcat
     2401 tttggtcaat tatataaaca tacctacaac tctgctttaa cctgttaatt tactacactc
     2461 ctttactttt tatacttatg tctatatatt tagacacgta tgacccccca actctactcc
     2521 gtcctccacg atattggcct gtctgaaaaa gaagccaaag tctacctggc tatgcttgag
     2581 ttgggagaat caagcattat ccccattgcg cgacacgcgg gaattaaacg caccaccgtt
     2641 tataactatc tggaagactt tactcgatta ggactcatta gtattaccat tcgtaatggg
     2701 cgccgatatt atgtagccaa tagccccaac aggttacgca ccatgctccg cagccgcctc
     2761 gaacaggtag aaacaatcat tccgaacctg ttttcgatgt ggaaagaaga ggaagagaaa
     2821 ccatcggttc aaatgttcga aggaattgat ggcctgaagc gggtcttcga gctctcacta
     2881 gagtgcgaga gcaaaaagat tgacgttatc cctattgaaa gcgctggtca tgtattcgtg
     2941 gggccggagt acatttctcg gtacatcgag aaatcgcgag aagccaacat tacgttccga
     3001 tcactgcgtg taccaagtga caagtatgaa aactatcgac acttcgaacc agaagagcac
     3061 gacaatcgaa ccataaaagt tgcccctgac tggtttacgc ctcaaagtta tattcaaatc
     3121 tatgataata acgtgggaat cttttcccac gcaaaagaga tcccctatgc gctggttatc
     3181 accagtcaaa gttatgccca cacccaacgc ctgttttttg aatcaatctg gcaacaatcc
     3241 accccaataa gggagtggct ccgagaaaaa gaaaaaactg agtaaaaaaa gcccgctgta
     3301 ccacgaatgg cacagcaggc ttaaaagctt gagcgaccaa gcgatacgac tactcaatgc
     3361 tcttgaagta cttgttgtga cggagcatgg agtaagtgct cagcaggagc aggtcgtgac
     3421 gacggagtat ccatttccag aacgtcaagt ggtggtacca gcttcgctcg cactgttctt
     3481 tggctttatc catgatccct cgttctttaa cgagcttaat catgtagtct tgccagcctg
     3541 aattttccac atctccatcg cgaaggacct ccaccatttg cggcacatcg agcagctcgt
     3601 gaaccgggta ggtgacaagg ccgttgcgaa catcggcaaa gcggtcttga taaaccttca
     3661 cgccgaggtc gcaggcgttc ggcaggaaat ccgcaatgtc gttaatgacc tgcatcgaat
     3721 gggagaaggg gatacagtta ccgaagaagt cgtagtggtg ccccacggta tcaagccgtt
     3781 cttcgtatcc ccgtacttcc gttcgtgcta tgtcatgtcc gatgagcggc gcccagtcgt
     3841 agacgaggtt tagggaggtg ttacgctctt gatacctttg agcccaccag aagcgactag
     3901 aagtcatttg attgagggga cggtgacgta cgctcagttc tcgctctact ccaaatccga
     3961 gcgtcatttt ttccgctaac tggctcatga gccacttgac tcgcttggtg tattgctcgt
     4021 ctagctgtga taaagatgtc cacctttcta tcaggtcgct ttgccacgtc tggagaaaga
     4081 gatagtccaa gagagcttgt tcaattcctc caggagcgtc ccagacagcc ttcttgtgat
     4141 ccaggatctg gttcgcagcg taagcgctgg tcatcacgag ctcaagagca atggcgagaa
     4201 gctgatgatc ctcccacctc acttctgtgc cgccaccagc taggtagcca gcgtagaatt
     4261 taagtccgat caggtgacag cgcttttgag tgtagcgacg cgtggctgag tcggacttgg
     4321 cgcaggcata ctgcgtagca aagatattga gcccggcaga caccttaccc atagtgcgcc
     4381 agtactcgcc tttaaaggcg ttgagttgcg gatcaagttc gggattcatg tactttcaag
     4441 gtcgcgagat atcgagcggt tgctacttcc tccgtcgata ggttggaatt atatacccga
     4501 agcatatttc gtcaactatt atgtcactat gcttttatac ccctacctcg taaatatcta
     4561 ttaacaggtt atttacatgc gaatttatct atgaaataat gacgacgtaa ataacgtcaa
     4621 tgatgcaaca tttaacctct atactcaatc aatttggctt ggccgataaa gaaagtgctt
     4681 tgtatttggc atctttgaca ttgggtgaat caggaatgtc ggagttagcg caaaaagcca
     4741 agctgaaacg gacgtcagcg tatgtggtat ttaaagcctt ggaacaaaaa ggcttaatgg
     4801 gaagcttcaa aatgcgatcc ggcaccaagt ttgtagctac accccccgaa gtagtcattc
     4861 gcaaaacaga acagcagcta atgcagctac aggctgtatt gccagaattt caggcaatcc
     4921 aagataaggc caacggccct aaaataacct acttcgaagg agaagaaggc taccgcgttg
     4981 cggccgaaga ctcacttaag tttgcgggcg aaacgattcg ccacatcggt tcacttgaag
     5041 aaattcataa ggttattagt agggattacg atattaatta ctacatcccc aagcgtattc
     5101 ataacagaat tcgcttaaaa gccctgtatt ttaaatcaga ttttaatgaa actaatttta
     5161 tgcgtgacga tggagctgaa ctgcgccaga ttcgtttttt acccgaatcg tttaaatacc
     5221 caacctcgct tttaatttat gggaataagg tggctttctt tagtactgcc aaagagttaa
     5281 ttacagtaat tattgaaagc gctgatttag tcgaaagcga acgtaagaag ttcgacttaa
     5341 tgtgggagct cttagacaac aaagaaacag gtgtttaaac ctgtttcttt gttggtagcc
     5401 cggagggtgt caatttgccc ggcgagccgg gaccaggcaa attgggtcgg cagagagtcc
     5461 gccaccgcgg atttatttgt acataccgac cgaacccgca taacttctcc cacccctcga
     5521 gttacacaac taaaaaagca ccggctaggg tgctttttta gttggtagcc cggaggggat
     5581 tcgaacccct gatcttcagg atgagaacct gatgtcctag gcctctagac gaccgggcca
     5641 gattcatctg tacccgtaca ttctactaga atactcgata tggttcaaga taaagatttc
     5701 ttaatattgc gcttagtgca aatatctaac agcaaagaat ccgcccacta aaagagcgaa
     5761 gaataataaa gcaaagagat cgaaatattt ctcgattttc tcttcaattt tttctccaaa
     5821 aacgcgtaac aggcctgcaa caataaaaaa tcgaccagca cgacctacaa cagaggcaaa
     5881 cacaagagtg ggaaatgaga ttttaaaaag cccggcggca atggtaatca ctttgtacgg
     5941 aatcggcgta aatgcagcgg taaggacggt cagaaaggcg ttatcggcat acttctgtcc
     6001 gaccaagttc accgttgact gcaagccata agcgtcaaca ataggctgcc ccaccgactt
     6061 atagagcccc ataccgatgg catatccaag catagcaccc aaaacagagc ccacagtaca
     6121 aatcaatgcc gtccgccacc aacgagcacg gtccgaaata accatcggaa taaggagcac
     6181 atcaggaggg ataggaaaaa atgaagcctc agcaaaggca ataacaaaga gcgctcgcga
     6241 agcgtgcttt gtcttggacc aatgaatggt ccagttatac agtttccgaa tagctcgtga
     6301 tggagcagta attaagcgta aggcagtcgt cacgttatcc tttcacatca agttcatgca
     6361 tgatcttacc acacagatcg attcgcccaa agagccttac ttccaagacg cttttcgctt
     6421 gaaaaaatat acgacaaatg gtattatcct aaccaattga cgcaccgcag aacgtctgcc
     6481 gtcaatgaca actttccaac acttccgccc ttaaaagggc ctcgacaacg caggcccctt
     6541 tggggtcagg agaacgcatg aaactcgtat tttggacgct cgtgtgcagc ctggctctgt
     6601 gcttcccgca cggatcctgg gcgaacccac cgatggttcg gggctatacg ctcccgagcc
     6661 atcggcagca accgcaccca cgcgcatcat cggctcggaa gaccaacgac gaaccccagg
     6721 ttcgtcatta caatctcaca gctccgaagc cgcggccgat cacaaaaacc aaaccgtacg
     6781 tggctcctcg gaaggaagag aaaaagatcg tcgtacggga gtacgacctt cgggacgtaa
     6841 atcccgttga tccgacccat cctctcaagg gactcgtgcc cgaggtaaaa gtcagtgagc
     6901 tggaaaagca cgtgggagag gcgacaaaga tcatcaccta caagctcaac cagaacaaag
     6961 acttcgcaac ggccaagagc accctcagcg aaagctgggg agcgattaag gagcgcgtgc
     7021 gacaggtagt tcgggaggag ctcaagaagc acccaacccg gacgaccacc aaggtcattc
     7081 gggaaacacg agttgtccaa ggcccggcag ctgcgccagc cgcagcgccc cgcattgatg
     7141 aggcagcgct cgaagcgagg atcacgaata atgtcctcgc caagatgcag aaccaagcga
     7201 agagtaaacc ggcacagcct gcgcctgcag aagagcggct caagaacttg gaagctatgg
     7261 cctacggcct ggtggccgta gtgttcgttc tcttcctagg attcctctac ttgcacaaga
     7321 ccaaggaaga taagtaaatg cggacacttc ttttgctcag cggcatcgct ttcgtcgttc
     7381 tcgtgatagg agggacgatc atctcggacg tgctcaagag aaggaggaac gcgggtctcc
     7441 cgagacgacg agtactcgat ccacccgtga cacctccaga agacatctcg tacgcctgta
     7501 cggttcgcct tccggacaaa cgggagttta ccgtccgaaa taaggcgggg gtaatattta
     7561 ccccaaacgt gcccacagag tgggaaggca atcccttctc gcacctgcac tcggaacacg
     7621 aaggagcgat caacaacttc tcacccgaga aggtgacgca acccgccgcc gacatcgttg
     7681 taacggctca ctataaggag aagaaaaagc cggggccttt cgaagaagtc gtcgtaccca
     7741 aagcacctcc ccgaccgtct attccaagac acgtggatgt gatccgggca gtcaacgact
     7801 tggacatccc ccccttcctt ctaaaagacc ccgtacgcct ggaagttaaa aatggcgagt
     7861 ttgtttacag agaccaattc agccaagtcg ccgagctcag acctggaaat actctcgttg
     7921 ctcgcgctta ccctggcctt gctgggtcga ctttctccca ctgggaagtc gaaggcgaca
     7981 tgaccctccc tcctagggag gaacacgtat cgccaatgtc tctaaccgta caggggaacg
     8041 caaccctcac ggcggtctgg aaggaactcg agactccccc tgctgaagaa gttccagagg
     8101 ccccacagga accgccccct agtcccatcg agcaggtcat cgaagagatg ctccccgagc
     8161 aaggaatctc tctcgagcca ccgggcgaga ccacaccagc cgaagcatct gctccggagg
     8221 tttcgccaga atctacggaa gaagaggagc caacgcagca gccagaaagc ggacctcagg
     8281 aagaggaacc cgccgctcca cccgaatctg cacctgtgga ggcaccggta gaggaggtta
     8341 ctaccgccga agaggacgat cagccagtag cggaagaaga agccgcaccc gtcactaatc
     8401 ctgacgcacc cgcgtcaggc aaaccggggg tcagaaaact tcctgccgga gctggcatac
     8461 gcaaaaaaca ataagtcccc cggagagcaa tctcccctta cgtccgagga atcggacaca
     8521 gccccaggcg gtaacgtctg gggttttgtt tttaacgttg gcgcgcgcat gaaagaatgt
     8581 agtagagtgg taataactca ggcaaaagga gaaccgtgga gcaagaacca aaccacgacc
     8641 cgcgtcaaaa gcttccagac tgggaagcag agggaccgga tcccgaagaa gtacgtgaag
     8701 ccgaagaaaa acagcgcgag gctaactttg aacatgaaaa gaaagttttt gagactaccc
     8761 actatttaaa tcatgtcgtc gaaacacagt tcgacggccg tacctacgaa gatctctcct
     8821 ccgaagacaa gctatatgca atcaccctgg ttaatcgtca gcataataca catcgcgtta
     8881 acacgcttgt ggcagagaga aatagtgggg ttcagggcct taaacataaa cttgatgata
     8941 agctaggccg cagtaaaatt tggggatttg ccaaaaatat tgccctagga gccggaggaa
     9001 gtttagccat gcggaccatg acggccggcc tggtcgcaac cggagggttt gttggcggcg
     9061 ctatctcggg agcaggcgca ggcatcgtca ccgggtcgta tcgaggatgg agtaatttta
     9121 ataaagaaca gctcaatttt cgggctctgg aaaacgagat tggcgcaaac gaacttatca
     9181 ccaaagggct tcatgagtca ccccaagaaa tcgagcaaga atacggcagc ctcgaggcat
     9241 actacagcaa agtgcaccaa gccttggcaa cctatcgaga aatcgagaaa agcaacctct
     9301 tgagcgataa tcgagaatac gtatctcgtt tttacgaaaa cttagcttca ctcgagagca
     9361 tttcgcagat acaaggaatt aagcaacaag cagagtcact cggtagagac ccgaccccag
     9421 aagaagcggc cgatctctta tgcaaagctc tcaatcaacg agaactcgcc aatgtcgagc
     9481 gatttgataa agggctcgct cagaaagctc aagagtttgt acgaacacga aaaagtgagc
     9541 gcctaaaaac aaccgtaaag cagggattaa ttggcggcgc cacgggcgct gtgcttggag
     9601 gtaccgttgg ttggctcctt gagcttgttt ttcactcccc tacagccgct ccaaccggag
     9661 agaaaataaa cattcccaac atcgcaggag gaggacacac cgaccccagc ttagctcaag
     9721 attacgccat tcataacaaa caatttcttg atgtgaccga agcaatccgc aacggcaata
     9781 cacaggaagc cattacccaa ggatggcaga ttcatcttaa cctcgatacc gatccttctg
     9841 tcgtcacccg acttcacgag attgctcaac agagcaatct ttcccccgac gaatataatc
     9901 gtttagctta taatgtttta acgtacgcga accgagaagg gattgataat aacgcgcttc
     9961 tgacgtatgc ccaacaagcg attacagacc ctcatgcgct tcaatctctt tctcctcaag
    10021 cacaacaatc catgcaagaa atatttggga ctacccacct tacacgagat agttttatca
    10081 atgcccatga gtttcgcaac ctttccacaa tcgatacatc aaccgacgcc tattatcacc
    10141 accttaaaga agctggagca ctcgcccaag caggaataga cagaaccgcc acactcgtaa
    10201 cgggttcttc tgcagccgcg acagtcccca agatagtaag caaattctcg cctccaaaag
    10261 agaaaataaa ccctgagcca cacgttgaag aagaagaaga agaagaagaa gtgaacgacg
    10321 aggcctatat aaatcaggaa cttacgtttg ataattttga aaactttaga atagccgagg
    10381 tgtctgtact tagcacagaa aaagaaatca gggcaacact acccatttct tcgggagaga
    10441 atgcgaacgt agtattgcat ggagatatca cagatccgga tacactaaga gagctcacgg
    10501 ccttaattga tcaaatagaa agcggagcca acaaacctac cctaacggcc gggtatacat
    10561 tcgtcgtttc gcgcgcagaa cgagacgcta gcaatacact ctccctctac gcgacactaa
    10621 aaaaaacata ttaaggagcc tgtatggaca cacaccctca tccttccacc cctttcccaa
    10681 ccgaccaagc agcccgcgag gttttaaagc ggctcaacat ggaagaaatt agtgacgaag
    10741 ccaaagaaag gctcctcacc cagattgctg atttaatcga ccaggaaatt ttgtaccagg
    10801 ttatgtctcg gctgagcaac gaagatatca ctgagttagg agaattctta cagacagacg
    10861 aaaacagcca cgaagagagc cttaaacccg aagccttcaa ttttattgcc tcaaagattc
    10921 cccacctcga tgtgattatt cgcgattcaa tcgtaaaggt atacgaagac attgtaagca
    10981 caaagaacct gctcgacgcc taaccttgtc caaacctgtt aaaccgctta taatgccaac
    11041 tatgcaagaa ccaggcattg tcgttgtcct tgataatatt cgttccgttt acaacgtcgg
    11101 tagcattttt cgcaccagcg acgctttagg cgttgcaaaa gtttatttat gcggcattac
    11161 ccccacaccc gaccacccac gcatgagccc ttctcaggaa cgcgactctt ttcataacga
    11221 accagataaa tacggccgga ccttttccgt cgctaaaacc gcccttgctg gccttcactc
    11281 ggtcgcatgg gaacacacac caagtacgct cgaaacagtt acccacctaa aaaagagcgg
    11341 ctacaccgtt atagcagttg agatcaccga cacaagtcaa cctattcacc ccatttcttc
    11401 tcgccctctg gcaattgttc ttggcaatga aaaagaaggt ataaatcaag aaattctcgc
    11461 cctggccgac gaaatactcc atattcctat gcacggcgtt ggcaaaagct tgaacgtttc
    11521 cgtctcctac ggcatcgccg gctacgaact actccgacag tctagtaatc cccaatagaa
    11581 tatgctacta tcgccgtaag ccataccaac aagggggaac agctatgggc gaaaaggcta
    11641 ttaagtggcc tgcgagcatc acactaatgc gccatgcagt atcggcgtac aacgacttga
    11701 agcggcagtg ggtcggagat ccggattacg aacgcttcgt aactctctac aacaccgact
    11761 ttcacaaccc agaaacgctt cgcttagcaa gggtaatcaa aagtcggtat gccctcggat
    11821 gcagtgacgc agatacgcct gcaaccaaag ccggccttga agacgccctg atcacaggat
    11881 ctcgcctcca cgaggtgatg gaactaccaa cgactatctt cgtctcgcct tacaagcgga
    11941 caaagcaaac gttggcggcc gtaatcaaag gctggccagc ccttggtaat gtccggatag
    12001 tagaggatcc tcggctccgg gaacaggagc acgggctctc aacggtctac aataactgga
    12061 tggtgtttca cgcccaccac cccgagcaga aggagttgta tgaccttgac ggttcgttcc
    12121 gttaccgtta cccgcaggga gagaacggtt acgatgtcca ggatcgagga cgtgacatct
    12181 tcggtacgtg gattcgagac taccgaggag aacggctctt cgtggtgact catcacctga
    12241 caatcctcac agtagcgacc ttaatcagtc gcggtggaga gcaagagttc aaggatttgg
    12301 ataagcacca caagccagtc aactgcggac tcacccagta cgtatgcgac cccgaccagg
    12361 ggaaggaggg aaaactgatt cagcggtatt ataaccgcaa gctgtactag agtcgccagt
    12421 gggcgactcg aaaacgggag cctccccagg ctcccgtttc gactttaaaa ctactttttc
    12481 ttatgctcgt ttgagaatac gtacactacc gtggttggcg cgtacctcaa ttaaatcacc
    12541 atcatgaatg agttgcgtcg catttttggt tccgataata cacggcttcc ctaattctcg
    12601 agacactata gcagcatgac aggtaatacc gccttcgttg gttacaatac ccgccgcttt
    12661 cttcataact ggcacaaact ctggccgggt catacctgtt accaaaatct cgccttcctc
    12721 aagcttgtca aattcagctc ggtccaaaat aaccttggcc acccctcgag cataaccttg
    12781 agaggcgata tttcccggta taaccacggg atcttcgttg gcttcgttga gcatttcttc
    12841 gaggtcatca cgaagcgagc tagcttcatc accttcaatc atcttgtagc tattcccctg
    12901 gataataacc aagccgccct gctcacagcg tttttttaac acatcaagac tcaccaatcc
    12961 ttttagaaca gcatcgactt cttcgaaact taaatactgc aggtagcgat acggaatacc
    13021 cgtctgcttt tgtaaggcct gcaaaagtcc atctaggaca tgaatccctt tcaaattaac
    13081 ctgttttcga tattcacgcc aaaaggtaag tttggcaaaa aattccaagg ggttgtgagt
    13141 taaacgatgt tttcgcaaaa cggctttttg cttcttaatc tgctgggcac tgtattgctc
    13201 taatcggtgc aactcggctg ttaacaagtc tttatcgaga aggtacttcg taagctcttc
    13261 tgccgtttcc tccagggtaa tatgactgat atgggcataa ttcgacttgt agtaatcaaa
    13321 agcttttata tactcagcca caccctcatc acccgcgatc acctctttca ctgccgttac
    13381 tgtcggcata cctaaggatt tgtgccgaga gagtcgttta gcaattttta aaagctcaat
    13441 gtaacgctca ttggtgaacg ctagttcttg cggcgttgta agcacaacaa tttcatcaat
    13501 ggttaaatcg tacttcttgg caataatctg aatttgctct tgatcaaacc cagcatcaaa
    13561 actatcaata aagagactat acacccaaaa ctggcgatac tggtcccaaa tgccatcact
    13621 aaattgccgg acctgactcg cagacatttg caagagagcc tctatatcta aattacgata
    13681 ggcggcctca aggctacggg catatggcaa aaaaatctga aaaagtaatt ccaagtcctt
    13741 tggggagtta tagcgacgat ataccccttg agacacccgc tgcatattgt agtaatccca
    13801 ataccccata ccataatctc gctgaaaata cgcatacaaa attgagtagt gatccgaaaa
    13861 aacggcctga tcaggttgtg tgatcgccat cacccacggc cagcaggtaa acggcacaat
    13921 agacccttct tgtttataga gcttgggagg cagatcgaaa tccgaccggc gctccgcgtc
    13981 gatctgcatt tctataagat catttaattt taaacgatag gtcttacaaa tctgctcaat
    14041 cgttccaaat ttaatgccat ccacctgatt gtggtagaga gcggtaattg tgttacgatt
    14101 taaccctgta tcgtgtgcaa tatcggtaat tgttttattc aattgttttg caattgtctg
    14161 caattttacc tgaatcatgg gaatactcct tgttcctagc tgtatgaaaa tccatcttct
    14221 ggggatggca cgcaccccca gaagcggttt aaaggtcgtt agtggcgtcg cgttaacgcc
    14281 ggcacataca cccactcggg accgaagtcc gagttgtatt caacttcgaa gtgcatcatt
    14341 tcgccgtagc ccaggcacgg gaaaccagga tcagccaaga ggtcgatcac cccttcgtga
    14401 ctcacaagaa tgacgaggcg atttggctcc acttcccgat cttgcggatt aagtacctga
    14461 tccatcagga agttaagaac ccgggtttgc acctggcgcg ccggaggata ttcctccaaa
    14521 tacacccctg cggtcagagt ctcgccggct tcgatacgcg cggctattct cgccttggct
    14581 tccgacaccg ggaactcggt ctcgttggct acgataatat cctcgcaccc aagctcgtta
    14641 gccatcacga tacgatccac tcggcttgaa aaggtcgaaa cgagctgagc cgccacctca
    14701 ttcgtgcgaa acgttgggct ccaccaaatc tcaatgctag caggtggatc gtcggcaatt
    14761 atctcgttaa tacgcccaac ggtctgagcc agcgtcgctt tgccctcctc gttgtgtagt
    14821 cctgttgccc tatcgtactg agcatgccga actccgataa ctcggggaac tcttctgtcc
    14881 atatttcagc accttattca cgtccacata cacgattagt atgttgggca tatagtacta
    14941 taagtaaaga aaattgtcaa tattataact cttgcaatag tcacaaggtt aatacaaaac
    15001 ggaacaacta gagattttct gcataacgaa aaaacagccc tggcgtgtgg gctgtttttc
    15061 catggctccg ggacaaggat tcgaaccttg gttatctgat ccagagtcag acgtcctgcc
    15121 actagacgat cccggaatga ttcgttatat caatgatacc tgcgtgaact tttctatgac
    15181 agttcgcaca taaaaccacg catttttcta tctctgcctc aactttcttc atgctccatc
    15241 cccatgattt gacatccgac agattaaaac ttttagtttt atcgtctaaa tgatgaaaat
    15301 caagacaaac tgagcttgat tctccacaaa cacaacagct cttggagctt tttaagaagg
    15361 ctaacttctc agcaattgac ttcttacgct caagtatacg ccctctttca agcaacctat
    15421 tattttcata atatgtacga ttcca
//
LOCUS       DASWYB010000050         9985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_489527, whole genome shotgun sequence.
ACCESSION   DASWYB010000050 DASWYB010000000
VERSION     DASWYB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489527"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1139
                     /locus_tag="VGE59_02930"
     CDS             240..1139
                     /locus_tag="VGE59_02930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY1074631.1"
                     /translation="MYPVQNRYGLGKNYRASPPPSQEHSHPWRTPTGVETRQAQSLEE
                     IQPFLAVPFERKLRENLRLSQTWKYNDQEAPLHPTIVLHKGCDFTHRLGTPIFAAARG
                     VALGSYHFTFLEDKFNRETKTRDMESGKRMGYGLGQFVLIWHPQMGVFTLYAHLQEIN
                     RQRIPYFLPEQDGEDWNPVVIMQSVAAMMASTNSQGYYPQWVNQGDPIGIMGVSGLSL
                     EGDGESPGFIPDQELFPTWDPAGPHLHFEVFTRKDDGSGKNLRWDPWGIYDEAHAYRG
                     VLESPTRGLFLPDPQTGWPKFAG"
     gene            1232..2068
                     /locus_tag="VGE59_02935"
     CDS             1232..2068
                     /locus_tag="VGE59_02935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY1074632.1"
                     /translation="MIRFLRQLFTLTLVLGIVYTSLIVWPKIQKRLLPCGRPITYDIG
                     QFDSQFNISQTEFLNSIQQAETIWEKSVSRDLFAYTPGSAFKINLIYDERQMTTEKQK
                     TLDNAIDNDIATLDEIKARYLSLEKTYTRELAQYNQDVSYWNSQGGAPKAEYDRLERQ
                     RASLNTQVQRLNSLSSRITTLASTANQKIDEYNTNAGQVFDKGVYQRAEGKEEINIYE
                     FKTPPELVLALTHELGHAIGLNHLSQETSIMYPVLSAQPLETITPTTEDITALKSLCR
                     IP"
     gene            2191..2958
                     /locus_tag="VGE59_02940"
     CDS             2191..2958
                     /locus_tag="VGE59_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074633.1"
                     /translation="MSRLFLFLLMGLLFIPLSAKAQYSDPRQDWWYFYRQPRNVGYYA
                     PAGGTYPTYVPTATPTPTPTYYPTAYPTAYPTAYPVAAPYAEAHATATATAADGTTAT
                     AEAHAVAKSDGYGYAPDSSYAQPYDQNGNYPIAFTLSGEPVYFVSANDAYYRFFPVNN
                     TTPADFCQTSWYWQWRWRVNQWRSPVASAEDYPLCQLRYNNQDFFYSPLNQQLVMDYN
                     APYTADPVVSRKLKIKEVIKTKNNKYVTKTVMKEKSR"
     gene            complement(2960..3541)
                     /locus_tag="VGE59_02945"
     CDS             complement(2960..3541)
                     /locus_tag="VGE59_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY1074634.1"
                     /translation="MTDKTHQMIGLTAATAIFLTTNPTPVTVSIAASVIIGSFIGSLA
                     PDIDQPTASLWRNIPLGGVWGRIVAECLGGHRNLSHSILGTFLFALLMNWLVQFIPPG
                     WGLDSHLVFSSFLIGFIAHLVADSVTVMGIPLLWPFGRNMGFPPYPFDGIRIITGKWF
                     ENLVILPLVTLSFIALVISYGSHFCAIFPPLCK"
     gene            3593..3925
                     /locus_tag="VGE59_02950"
     CDS             3593..3925
                     /locus_tag="VGE59_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HEY1074635.1"
                     /translation="MRIWDVTPRCLCRQHLLGEHRELHALWSIIQNHKTGYQSHPETK
                     RWVGKLAALHKRHDDLVNEMLRRGYTHSSPLPKPPKDISVQTDFLQSEEEQYQILRTK
                     NCPCPLDS"
     gene            3941..4387
                     /locus_tag="VGE59_02955"
     CDS             3941..4387
                     /locus_tag="VGE59_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY1074636.1"
                     /translation="MASKHLYRSRDNRVIGGVAGGLGEYFDLDPIVIRLLFVLLAVGG
                     GSGVLIYIIAWILMPEEPTVTKEKNSEKSFEETIESNVKDMADKVEKSAKKIHERSSV
                     SGGLILLVIGVLFLLQNMTGFDVWGNFWPIILVVIGLGLIARSSKS"
     gene            4397..4726
                     /locus_tag="VGE59_02960"
     CDS             4397..4726
                     /locus_tag="VGE59_02960"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEY1074637.1"
                     /translation="MVTLILGLIITALGLSYLGTNLGLWASFNWMNVWALWPLILILI
                     GVRLLVRSNRLFALGAFIILGLALRMLILAGDHPLMQSGFGSGVWDCQWANHLPDISR
                     MHRLQLH"
     gene            complement(5465..6124)
                     /locus_tag="VGE59_02965"
     CDS             complement(5465..6124)
                     /locus_tag="VGE59_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1074638.1"
                     /translation="MKQFDFYTSDGVLLSGIANEPKEVTKRILCLHMMPAEKESYLGF
                     MEKVEPLGWLCHAIDFRGHGSSTKQGDETLDYHDFSAEGHKKYMIDASESLSMLREGK
                     AVDAVIGASIGANVALRLQEQESVPYAVLLSPGTDYHGVTTLDVAGKLTSNQAVYIVA
                     TKEDERAAGNAEAMAKEIFEKLSTTKKKIDIYGGSKHGTDILDGYGERMDKIIEFLQG
                     K"
     gene            complement(6121..7677)
                     /locus_tag="VGE59_02970"
     CDS             complement(6121..7677)
                     /locus_tag="VGE59_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1074639.1"
                     /translation="MQPKTRLYRFFEILPGTLMWLTFILPVALAFTYPRVVSLAIMIY
                     ALLWFFKTVVMSRHLILGYYGYRDAMKKNWYHEMVSSHPKEWERVTHVAFVPMYKESF
                     EIVAATFDALIASDYPLKKVIPILCTEGRAGEEAQEVAKKIKKKYAHYFPHFFVTVHP
                     ADIPGEVKGKGPNMNWAAKEVIPQLLSSGYKAKDILVTTLDADNRVDRHYFACVTKAF
                     LESPDPAHTSYQPLPMYFNNIWDVPLFVRMIALGSSFWVMVQATRADRLRNFSAHSQS
                     LEGLIEADYWSSTSIVEDGHQYWRSLYAFNGNHTVVPIFVPIYQDAVLGATLKDTIKE
                     QYLQKRRWAWGVSDVAYVFTNNLKDSNMSFWEKWKQFFILLEGHYSWATTSLVLALGG
                     WPPLLINAEYQNTVFAYNFPFFYSKILLAAGIGMIITLVISTLVLPPPPRRYKNHKLI
                     VIRDWILTPIILPITNIFLGALPAIDSQTRLMFGRYLEFRVTVKRMVDTVVTPETKKT
                     PAPKSAKEIV"
     gene            complement(7679..7975)
                     /locus_tag="VGE59_02975"
     CDS             complement(7679..7975)
                     /locus_tag="VGE59_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074640.1"
                     /translation="MVSNTFLWRWVFKDRIFVALLLLSTVTFLMAIAERHPSFERTSE
                     PFHLGLLFFILNTVFAVLALRREPLLSYMFLTASVLMNGTLFFFFRYLIIIQGA"
     gene            8068..8141
                     /locus_tag="VGE59_02980"
     tRNA            8068..8141
                     /locus_tag="VGE59_02980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8100..8102,aa:Gly,seq:ccc)
     gene            complement(8482..9030)
                     /locus_tag="VGE59_02985"
     CDS             complement(8482..9030)
                     /locus_tag="VGE59_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY1074641.1"
                     /translation="MNDLIAVHSPEVLREQLLELVDPADQELVAGAVDFAMNAHALQR
                     RKDGTPYVLHPLRVAVRLAKDGYDNSNLLAIALLHDVIEDCEITRDELAEAFSSLVAD
                     GVQQLSKPRRSSDYFIELQHASEQLKLIKIYDRLDNLATMQPFSPEKRQQYINETETY
                     FFDLSQFSPLLQEKLESAISAW"
     gene            9183..9257
                     /locus_tag="VGE59_02990"
     tRNA            9183..9257
                     /locus_tag="VGE59_02990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9215..9217,aa:Thr,seq:cgt)
     gene            9372..9448
                     /locus_tag="VGE59_02995"
     tRNA            9372..9448
                     /locus_tag="VGE59_02995"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9406..9408,aa:Asp,seq:gtc)
     gene            9545..>9985
                     /locus_tag="VGE59_03000"
     CDS             9545..>9985
                     /locus_tag="VGE59_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074642.1"
                     /translation="MKKDIQLHPAQLAILRELLYKPEARFSELNVTNLTSDHFNFHIQ
                     QLIHAALVEKSEGAKYKLTSSGKEFANRFDTDTPNNITIEKQAKIGVAVCCVDTQDGE
                     IKYLLQQRLKQPYYGYWGCMTGKIKWGESVYETAKRELEEEMGVQ"
BASE COUNT         2888 a         2457 c         2223 g         2417 t
ORIGIN      
        1 gtggccgtag gctcaccaaa gtttatttct aaaaacgata ggttggcggc ctgaaccgac
       61 aaaaggttaa ggggaattgc caaggctatc ccagcaagta gagcaaaaaa tcgtcgcatg
      121 tccctcctct ttctataaaa acagcatacc gttttttcca aacattgacg aatgcatgct
      181 tgtgagagcc cggttaaaag agtataatcc atttagcgca acgactccgg agtatcacca
      241 tgtatccagt acagaaccgt tacggtttag gaaaaaacta cagggcgagt cctcccccat
      301 cacaggaaca ctcacacccc tggagaacac caacgggtgt ggaaacccgc caagcgcaaa
      361 gcttggaaga gattcagcct tttctcgctg ttccgttcga gagaaagctg agggagaacc
      421 tacgcctgtc gcagacgtgg aaatacaatg accaggaagc gccgctgcat ccgacaattg
      481 tgctccacaa gggatgtgac tttacacacc ggctcggtac acctattttt gctgcggcaa
      541 gaggcgtggc gcttggttcg taccacttca cattcctgga agacaagttc aaccgggaga
      601 cgaagacccg cgacatggaa tcaggaaaga ggatggggta cggccttgga caattcgtcc
      661 tcatctggca tcctcagatg ggagtcttca cgctctatgc ccacctccag gaaatcaatc
      721 gccagcgtat accctacttc ctgccggaac aggacggcga ggactggaac ccagttgtga
      781 tcatgcaatc ggtcgcggcg atgatggcgt cgaccaactc ccaagggtat taccctcaat
      841 gggttaacca aggtgatcct atcgggatca tgggtgtttc cgggctctcc ctcgagggag
      901 acggcgagtc tccaggattc attccagatc aagagctatt tccgacctgg gatccggctg
      961 gcccccatct ccacttcgag gtattcaccc gtaaggatga cggatcgggc aaaaatttgc
     1021 gctgggaccc ctgggggatt tacgacgagg ctcacgcgta tcgaggggtt ttggagtcac
     1081 ccacacgtgg gctttttctc ccggatcccc agactggctg gccgaagttc gcgggctaga
     1141 ccagagattc ttccctgact gaagacgggg ctgcacgtgt gcagccccgc tttctttttt
     1201 attactctta aactccgtat actaaaatta tatgatccgt ttcttaaggc aactatttac
     1261 ccttaccctt gtgcttggaa ttgtctatac gtcactgatt gtatggccaa aaatccaaaa
     1321 acggcttctc ccctgtggcc gcccaattac ttacgacatt ggacaattcg attcgcaatt
     1381 taatatctcg caaactgaat tcttaaattc tattcaacag gcggaaacaa tttgggaaaa
     1441 gagcgtttca cgggatcttt ttgcctatac gccaggttct gcctttaaaa ttaatttgat
     1501 ttatgacgaa cgtcagatga caaccgaaaa acaaaagacc ctcgataacg cgattgataa
     1561 cgatattgcc actctcgacg agattaaagc acggtacctt tcactcgaaa aaacctacac
     1621 tcgggagtta gcgcaatata accaagatgt aagctactgg aactcacaag gcggcgcccc
     1681 taaagccgaa tatgaccgtc tcgagcgtca acgggccagc cttaataccc aagttcaacg
     1741 gctcaatagt ttaagcagcc gaatcaccac acttgcctcg acggctaatc aaaaaatcga
     1801 cgagtacaat acgaatgcgg ggcaggtatt tgacaaaggt gtgtatcaaa gagctgaagg
     1861 gaaagaagag attaatattt acgagttcaa aacacctccc gaactagtcc ttgctttaac
     1921 gcatgaactc ggccatgcta ttggactcaa tcatctgagt caagaaacct caatcatgta
     1981 cccggtctta agcgcccaac ccctcgagac catcacacct accaccgaag acattactgc
     2041 gctaaaaagc ctgtgtcgta taccttaatt tagggaaaac actaacaaaa gaaatcaatt
     2101 cactttgtga atggacaaga tagataattt actttcgcaa atttcctgct agggtaagag
     2161 caacgatcaa agattgcgta aggagttact atgtcccgct tattcctttt ccttttgatg
     2221 ggtcttttgt ttatcccgct gtcagcgaaa gctcagtata gcgacccaag acaagactgg
     2281 tggtattttt atcgccaacc ccgaaacgtt gggtactacg cccctgctgg tggcacgtat
     2341 cctacatatg ttcccacagc gacacccact ccaacgccaa cgtattatcc caccgcatac
     2401 ccaacagcgt atcccacggc ctatcctgtc gcggcaccct atgctgaggc acacgccacg
     2461 gcaactgcta cagccgccga cggcaccacc gcgaccgccg aagcccacgc ggtagcgaaa
     2521 agcgacggat atggctatgc gccagattcc agctacgcgc agccgtatga tcaaaatgga
     2581 aattatccga tagcatttac cctgagcggc gaacccgttt actttgtctc ggctaatgat
     2641 gcctactacc gattcttccc ggttaataac actacacctg ccgacttttg ccaaacctcc
     2701 tggtactggc agtggcgatg gcgagtaaac caatggcgct cacccgtggc cagtgcagaa
     2761 gattacccgc tgtgccagtt gcgctataac aatcaagact tcttctattc cccgctaaac
     2821 cagcaactcg tcatggatta caatgcccct tacacggccg atcctgttgt atcacggaag
     2881 ttaaaaatca aagaagtcat aaaaacaaag aataacaagt acgtcaccaa aacagtaatg
     2941 aaagagaagt ctcgctaaat tatttacaca gaggcggaaa aatcgcacag aagtgcgaac
     3001 cataactaat aactaaggca ataaaactaa gggtcacgag cgggagtata accaagttct
     3061 caaaccactt cccagtaata attcgaattc cgtcaaacgg atatggtggg aaacccatat
     3121 ttcttccgaa tggccacaaa agagggatac ccataacggt taccgaatca gccactaagt
     3181 gagcaataaa tccaattaag aagctcgaga aaacaagatg cgaatctaat ccccaccccg
     3241 gtgggataaa ttgtacgagc cagttcatca agagagcaaa aagaaacgtg cctaagattg
     3301 aatgggataa gttacgatgg ccgcccaagc attcagccac aatacgtccc catacgccgc
     3361 caaggggaat attacgccac agcgaagccg tcggctgatc gatatcaggg gcaagagacc
     3421 caataaacga tccgattata acgctggctg cgatagaaac cgttaccgga gtcgggtttg
     3481 tcgttaagaa aatcgccgtt gcggccgtaa gacctatcat ttggtgcgtt ttatctgtca
     3541 tagttctagc ctatcattag gacaaaaaga aaacacctat tataatataa gtatgcgaat
     3601 ctgggatgtc actccacgtt gtttatgtcg ccaacatctt ttaggtgaac atcgtgaact
     3661 tcatgctttg tggtcaatta tccaaaacca taagacaggc taccaaagtc atccggaaac
     3721 aaagcgctgg gtagggaaat tggcagcttt gcataaacga cacgatgatc tggtaaacga
     3781 gatgctacgg cgaggctata cccatagcag cccactccct aagcctccca aagatatttc
     3841 tgtacaaaca gactttcttc aaagcgagga ggagcaatac cagattctcc gtacaaaaaa
     3901 ttgtccgtgt ccgttagatt cataaagaaa ggaatgcgct atggcaagta aacacctcta
     3961 tcgctcgcgc gataatcgag ttattggcgg ggtcgccgga ggactcggtg aatactttga
     4021 tctggatcca atcgttattc gactgctctt tgtcctgctt gcagtcggtg gaggatcggg
     4081 cgttttgatt tatatcattg cgtggatcct catgcccgaa gaacccacgg taacaaaaga
     4141 gaaaaatagt gaaaagagtt ttgaagagac tattgagagc aacgtcaaag acatggccga
     4201 caaagtcgaa aagtcagcca agaaaatcca cgaacgcagt agtgtaagcg gtgggctgat
     4261 tcttctggta attggcgttc tcttcctcct ccagaacatg acaggattcg acgtctgggg
     4321 caatttttgg cccatcatat tggttgttat cggacttggg ctgattgccc gaagcagtaa
     4381 gagctaaggc gcacgtatgg taacccttat tttaggtctt attattaccg ccttgggcct
     4441 ttcctacctg ggcacgaact tgggactatg ggcttcattc aactggatga acgtgtgggc
     4501 actctggcct ctcattctca tcctcattgg agtacggctt ttggttcgca gcaatcgtct
     4561 cttcgctctt ggcgcattca tcattctcgg cttggcctta cgtatgttaa ttcttgccgg
     4621 agaccaccca ctcatgcaaa gtggattcgg ttcaggcgtt tgggactgcc agtgggcaaa
     4681 ccacctaccg gatatatcaa gaatgcatcg gctgcaattg cattagtctt cataaacaaa
     4741 aataagccgc gagtaacttt gctcgcggct tatttttttg tttgcaagag agaaacacaa
     4801 gttacttgca taaaataaaa aaccgcccga ggcgcagacc agcaaggaaa agccttgcca
     4861 ccctaccctc gggacagccg tcggataccg ttcttcagta atattcatgc taagtaaccg
     4921 ccaccagcag ttaatttcat gctttctgtt aaacggtaac catctcagag gttttctcgt
     4981 gatgtggtac caacatcagt gacgtaactt ttccggaaaa agtgctgtca caccagtcac
     5041 ctccttggag gaagcgctgg gagggttacc tcaccgccag gatgcagcat tgagcacttg
     5101 aacgtatgaa ccttgcggtt gcgattggct caaactggga ctcgggtgag tcacacctgg
     5161 agagtagctg gttagccgca atgagcgacc gcgcagttta ctcaacgtta cttttggggt
     5221 aacgagcacc atccacggga gacgaacagc ctttagcgct acgctcgaaa gccacccgcc
     5281 gcacgtcggt cctaacggac gaacgcccga catctcggtg ctatttacta cgctacagac
     5341 ttcaaaaatg cttgtcaata ctattacgcc accaatccgc caatgctata aaattgagca
     5401 tatacgcagt atgtctcata tcaaaaaaga cccctgttat tgcgcagagg tctttttatt
     5461 ttgcttattt tccttgaaga aactcaatta tcttgtccat tcgctcgcca tagccatcga
     5521 gaatatccgt cccgtgcttt gagcctccat aaatatcgat tttctttttc gttgttgaaa
     5581 gcttctcaaa aatttctttc gccattgctt cggcattccc cgccgcccgc tcatcttctt
     5641 tcgtggcaac aatataaacc gcctgattcg acgtaagttt gcctgcaaca tcaagggtcg
     5701 ttacgccatg gtagtcagtt cccggcgaca gtaaaaccgc gtaaggaaca ctttcttgct
     5761 cttgaagacg taatgccaca ttcgcaccaa tactggctcc aataaccgca tctaccgctt
     5821 taccttcacg aagcattgag agtgactcag aagcatcgat catgtacttc ttatgtccct
     5881 cggcggaaaa atcatggtaa tcgagcgtct catcaccttg ttttgtactg cttccatggc
     5941 cgcgaaagtc gatcgcatga cacagccagc caagcggctc aaccttttcc ataaagccga
     6001 ggtaactctc tttctcggcc ggcatcatat gtaaacataa aatcctcttg gtaacttctt
     6061 ttggctcgtt agcgatacca gagagtaaca cgccatcgct cgtataaaag tcaaactgct
     6121 tcatacaatc tcctttgccg acttaggtgc cggagttttt ttcgtttcag gcgtcacaac
     6181 cgtatcaacc atacgtttca ctgttacccg gaattccagg taacggccaa acattagccg
     6241 cgtttgagag tcaattgccg gaagcgcacc taagaatata ttagtaatcg gcaaaatgat
     6301 cggcgtcaag atccagtctc ggatcacaat caatttatgg ttcttatatc gccgtggagg
     6361 cggtggcaat accagagttg aaataacgag cgtaataatc attccgatac ctgcagcaag
     6421 caggatctta gagtaaaaga agggaaagtt ataggcaaac acggtatttt gatattcagc
     6481 attaattagc agtggaggcc aaccgccaag ggctaatacc agggaggtgg ttgcccacga
     6541 gtagtgcccc tctaagagaa taaagaactg tttccatttc tcccagaatg acatgttgga
     6601 gtctttgagg ttattggtaa agacatacgc cacatccgat acaccccacg cccaccggcg
     6661 cttttgtaaa tactgctctt taattgtgtc cttgagcgtt gcgccgagca cagcgtcttg
     6721 atagatcggc acaaaaatag gaacaacggt atggttacca ttgaaggcat agagtgaccg
     6781 ccagtattga tgtccgtctt caacaatcga cgtactcgac caatagtctg cctcaatcaa
     6841 cccttcaagg ctctggctat gggccgaaaa gttacgtagt cggtcagcac gagtcgcttg
     6901 aaccataacc caaaacgaac ttccaagcgc gatcatccgc acaaagagcg gcacgtccca
     6961 aatattgttg aagtacatgg gcaaaggttg atagctcgta tgagcagggt cgggtgattc
     7021 taagaacgcc ttggtaacac aagcaaagta gtgacggtca acccggttat cggcatcgag
     7081 cgtcgtcact aaaatatctt tggctttata ccccgagcta agaagctgag gaataacttc
     7141 cttagccgcc cagttcatgt ttggcccttt tccttttact tcacccggga tatccgctgg
     7201 gtgaacagtc acgaaaaagt gagggaaata atgagcatac ttctttttta ttttttttgc
     7261 gacttcttga gcttcttctc cagcacgccc ttcagtacag agaatcggga taaccttttt
     7321 gagtggataa tcactggcaa ttaaagcgtc aaaggtagca gcaacaattt cgaagctctc
     7381 tttatacatc ggcacaaatg caacatgggt tacccgctcc cattctttag ggtggctcga
     7441 aaccatctcg tgataccagt tctttttcat cgcatcgcga tatccgtagt atccgagaat
     7501 aagatggcgc gacataacca cggttttaaa gaaccaaagc agtgcgtaaa tcatgatggc
     7561 cagagacaca actcgagggt aagtaaaggc aagggcgacg ggcaaaataa aggtaagcca
     7621 cattaacgtg ccagggagaa tctcaaagaa gcgatagagc cgagttttag gttgcatatt
     7681 acgctccttg aattatgatt aagtaccgga agaaaaagaa cagagtccca ttcataagca
     7741 ccgatgcggt gagaaacatg tacgacagta acggttctcg tcgtaatgct aaaactgcaa
     7801 aaacagtatt aagaataaag aagagaaggc caaggtgaaa gggctcggac gttctctcaa
     7861 aagaagggtg acgttcggca attgccatca aaaaggtaac cgtactcaat aataagaggg
     7921 ccacaaaaat acgatcctta aaaacccaac gccacaaaaa agtgttcgat accatatgtt
     7981 ctttttatac taacacgccc atcagaagta tttcaattga agagagcttg tcaatttgct
     8041 aaaataaacc tataaatcat ccttcgtgcg ggtatggttc aatggtagaa tgcgaccttc
     8101 ccaaggttga gacacgggtt cgattcccgt tacccgctcc aagcgagaga caccctaaac
     8161 gggtgttttt cgtttggaga agataggaag atcggacccg acgagttcga tgacacaccc
     8221 aaagcgaagc agaggaaagt catgctgagc cgtcactatg aatttctgac ggtgaggata
     8281 ttcccgttac ccgctccata caagaagtga cctcacggtc gctttttgtt atatgtaagg
     8341 tagtattata gtaaatccac aaggagcact ttatgaaaaa accaatcgtt ccagcctgcc
     8401 caacgtgtaa tattcccctc cccgcaacta aaatatgtac aacctgccag gctggcagca
     8461 atcacgaagg agaatgagga gctaccaagc cgatatcgcc gattctaatt tttcctgcaa
     8521 tagcgggcta aattgtgaca agtcaaaaaa gtaagtttcg gtctcgttaa tatattgctg
     8581 acgtttctcg ggtgagaagg gttgcattgt cgccaaatta tcgagtcggt cgtaaatttt
     8641 aatcaattta agttgctcag aagcatgttg gagctcaata aaatagtcag agcttcggcg
     8701 cggtttgctt aattgctgta caccatcagc aacaagagac gaaaaagcct cagccagttc
     8761 atcccgtgta atttcgcaat cttctatcac gtcatgcaaa agggcgattg ccagcaagtt
     8821 agaattatcg taaccgtctt ttgcgagccg caccgcaacc cgcaacgggt gcaatacata
     8881 cggggtccca tctttacgcc gctgtaaagc atgagcattc atggcaaaat caacggcccc
     8941 agccacaagc tcttgatctg ccggatcaac aagttcaagc agctgttcac gtaacacttc
     9001 tggagagtgg actgcaatca aatcgttcat gctctcggaa aacctaaaaa gagataaatc
     9061 aaataagcga ggatagaaag ggcgcccgca attataaata ccaaatatcc agaactatat
     9121 gggttcttag gcattcactt ttcctttcct tttttgtata ataacagggt ataaattaat
     9181 gtgccacctt agctcagcgg tagagcatct ctttcgtaaa gagggggtcc ggggttcgat
     9241 tccccgaggt ggctccaggc agctcaaagc cccgaaggcg gcgagctgca ctatattctt
     9301 gatgaggtag cgcagaaaag gtataatagc agaagtttaa cgaagtaaaa ccttcgtaag
     9361 acaatcatcg tggacccgtg gtgtagcggc ctaacatgcc tccctgtcac ggaggagacc
     9421 gccggttcga atccggtcgg gtccgccatg aagaagccga ggtgcaaacc tcggtttttt
     9481 catacttaaa aacactaaag ccaactttac tagatatttt gcagacactc cctacaatga
     9541 agctatgaaa aaagatatcc aactccatcc ggcacaatta gccatccttc gcgaacttct
     9601 gtacaaacca gaagctcgat tctcagaact aaacgtcacc aaccttacaa gcgatcactt
     9661 taattttcat attcaacaat taatacacgc tgctctcgta gaaaaaagtg aaggtgcaaa
     9721 atataaactc acctcttcgg gaaaagagtt tgcgaatcgt ttcgatacgg atacgccaaa
     9781 caatattacg atagaaaaac aggcaaaaat tggagtcgcg gtttgttgtg tagataccca
     9841 agacggggaa ataaaatatc ttcttcaaca acgcttaaaa caaccttact acggctactg
     9901 gggctgtatg acgggaaaaa ttaaatgggg tgaatctgtt tacgaaacag ccaaacgaga
     9961 actcgaagag gaaatgggag tgcaa
//
LOCUS       DASWYB010000051         9705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_595137, whole genome shotgun sequence.
ACCESSION   DASWYB010000051 DASWYB010000000
VERSION     DASWYB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595137"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VGE59_03005"
     CDS             complement(<1..437)
                     /locus_tag="VGE59_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1074643.1"
                     /translation="MRGFTLIETLLYSGLVVIILGITITASLTFTNASLRDKKRSEVV
                     ANERFVREKTDWLFSGLESNDVINPLTSGGTLDATLTSGQRIRLQLTGAALELVRDTN
                     GDGAITAADTKSALTNQYVTVSDLTFTRETWNGQPTFVMTCHMA"
     gene            complement(448..1857)
                     /locus_tag="VGE59_03010"
     CDS             complement(448..1857)
                     /locus_tag="VGE59_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074644.1"
                     /translation="MTGKRVAGSALIELLIASGVFLVGTLSLFTLVLGSGPLILETTR
                     NKQATDYAREGIEIARLIRDQNWDGLKSGTYGLEFVNGNWTLSGASSQSDTFTRSITI
                     AEVSAYSKRVTSTVSWVPGGNRNATISLETLITNWHSTQQENYIAGDWVHPLSAASAD
                     IDSGAAGTDVFVENQKAYVSSQSTNVTKVDFSTFAVQNPSSPTLVSSVDLGISNVASI
                     AKNGNYVYGSVADSTKEFVIIDISNPAAPIKVGDVDVSNGKTRSITVSGNRLYLGMER
                     IAGESEFYIYDVTAPTSPVKLGSFEIDGTVNSIAVLNGRAYLATSHDTAELMILNVAD
                     PAAIAVLGTYDAPDVYDGMNVSVKDEFNIYLGRANSPTQPEFLILSATNPVSPTVQGT
                     FDLNTGINDMVIVNRLAFLATELPNAEFKVLDVANPAQISAYSSLNFPQSVTGIAYEN
                     NTIYCSVRSNGALRVITPS"
     gene            complement(1847..2311)
                     /locus_tag="VGE59_03015"
     CDS             complement(1847..2311)
                     /locus_tag="VGE59_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY1074645.1"
                     /translation="MKQSFSGFTLIELIVVIGIFSMLLLVSLPFTLNSIVSAQLNRER
                     DTLIPLLQSARDQSYANLRGRDTGVKINSSNYTLFEGTTYASRDTSHDIVYPRSEQLF
                     ASGTEFRFRAPDGVPTIVSGGVSKSSYSENFVDPKTGRNFTLSINQEGMIDW"
     gene            complement(2315..3355)
                     /locus_tag="VGE59_03020"
     CDS             complement(2315..3355)
                     /locus_tag="VGE59_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1074646.1"
                     /translation="MKVPIKEQILFARNISLMSRSGIPILQSLHLVAQQTASPGMRKV
                     LTVLITDVENGQFLSTSLKKFESVFGSLFINIVRIGETSGTLSENLGFLADELKKKNE
                     LRGKILSAMMYPIIIVITTILLMAILTFAVFPKILPIFESFQVPLPLTTRIVIGSNKI
                     MLAYWPYILIGILVVVGLWFLLIRLPRFRFLFQMFLIRLPIIGPLTMSIQMAIIARTM
                     ALLLKSGVKIVEALLITGDIVPNLVYRQLIAAAGEQVKAGSPLGRYFANYPQYVPLMF
                     SQMLDVSETAGTMDATLLYLSDYYEAEVDETTRNFVSLLEPLLMLTMGGAVGFIAISI
                     ILPIYSISQNFG"
     gene            complement(3372..4715)
                     /locus_tag="VGE59_03025"
     CDS             complement(3372..4715)
                     /locus_tag="VGE59_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY1074647.1"
                     /translation="MAEVTSPIVSTPSEEVSKTSHIPLVLSNMTDEERAKTTATVESI
                     KTFIEQELSRQPSEFSIARLIDLLIAYASLTRTSDMHIDPTPENVRVRFRVDGILYDV
                     FSLPKIIQSLLVARTKILCGLRTDEHQVPQDGRFKVLLKDIGTVDLRVSIVPTYYGEN
                     VELRLLQSPSEKLTIDGLDMSPSDLAKVKRAMTRPYGMILATGPTGSGKTTMLYTILK
                     TLNTPDVSVITIEDPIEYSIDGIEQIQINPQSGLTFAAGLRAILRQDPNIIMVGEIRD
                     EETAEIAVNAALTGHLLLSTLHTNDAATTLARLTEFKIEPFLIASTVNLAIGQRLVRL
                     LCPYCRIPKTITEAEYQSLAEVIPIAFLGDNRQFFAAQGCEQCGQSGFIGRTGIYEVL
                     LVDDAIREAIMRRANANEIVALAMQKGMTTMLQDGFRKALEGKTTVEEVLRVVHE"
     gene            complement(4900..5817)
                     /locus_tag="VGE59_03030"
     CDS             complement(4900..5817)
                     /locus_tag="VGE59_03030"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEY1074648.1"
                     /translation="MKITKAIILAAGYGTRFLPITKAMPKEMLPVVDKPVIQYAVEDV
                     VSAGIKDIVLVTSAQKRPLEDHFDHTFELETVLEKGGKVNELEQVRAIANLANFIYVR
                     QKKIKGTMGAFSYGYKAMPNEPFVATWGDDFFVASPTRSQQLVAAYEKYGAPILGCVR
                     TTNPEHGSRYAFAEGDEIEPGVLRVRRIIEKPGTGQAGSEYACVSGFVFTPELMTYAD
                     KIEPMKNGEYGYSDAVAAMVADGKPVYAMTIQNGRYYDCGNKLDYIKTNIELALQHPE
                     MREELSAYLRDLTKTELLKVAPDHAKVVI"
     gene            complement(5833..6216)
                     /locus_tag="VGE59_03035"
     CDS             complement(5833..6216)
                     /locus_tag="VGE59_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY1074649.1"
                     /translation="MAQKTIGQDEHWMNHVEDEGQLAVDVYQTEDSIVLVAPIAGVNE
                     ANLEVAITDEIISIRGSRSLNEQVPSENYFVQECYWGSFSRSYVLPVAVDPDAASAGL
                     KDGILTIRIPRVEKSKTRILKVTSI"
     gene            6584..7000
                     /locus_tag="VGE59_03040"
     CDS             6584..7000
                     /locus_tag="VGE59_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY1074650.1"
                     /translation="MTSVELALSQLIRELPLPRHAPVIVTSVPSSPSALATRGFNQSE
                     LLAKSCARTTGTLCYVPLLKRTNRSTSQTRLTRKARFANVVGQFSLARAIPSLNNTTI
                     IIIDDVITTGATLAACATVLKEAGAKAVWGITVAKD"
     gene            complement(6981..7898)
                     /locus_tag="VGE59_03045"
     CDS             complement(6981..7898)
                     /locus_tag="VGE59_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY1074651.1"
                     /translation="MSTNSFTPLQASVLRWYEKNGRHSLPWRNVADLGVDPGYGVMVS
                     EFMLQQTQVERVIPKYTAFLKRFPTVESLASASVSDVIALWSGLGYNRRAVLLHRAAQ
                     TIHTLYSDDVPHGIDTLKSIPGIGPYTAGAIAAFSYNEPVAMVDTNIERFYELLFWGY
                     EKPSVKEVATFAQSYVPSACSRAWHSAIMDLMTCVRKRRTPLEQQQSLLEELAFIPRW
                     TLPLLGSKPFARPKQSSFVGSRRSFRGRIIAYLTSCENHQASLSQVGDILPSDMPYKL
                     EELIEGLVKDGLLTILPGSTEPILSLSPQ"
     gene            7973..8048
                     /locus_tag="VGE59_03050"
     tRNA            7973..8048
                     /locus_tag="VGE59_03050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8006..8008,aa:Arg,seq:gcg)
     gene            8312..8788
                     /locus_tag="VGE59_03055"
     CDS             8312..8788
                     /locus_tag="VGE59_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074652.1"
                     /translation="MKAPSRTSFKPISWRAPEYLFRRKTLLWYTHVSVFFFLVLVLLF
                     WIQNWFGVVILVLLFWLFMSKSEEKPKNVTYTIDHHGITIGDRTLTFSEIESFTAEIV
                     HRTPIITLNLTYSFALPITLIVKHDVKDEVIDHLSQYIPLSNDFSFMRWLSHRLHY"
     gene            8845..8930
                     /locus_tag="VGE59_03060"
     tRNA            8845..8930
                     /locus_tag="VGE59_03060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8879..8881,aa:Ser,seq:tga)
     gene            9014..9092
                     /locus_tag="VGE59_03065"
     tRNA            9014..9092
                     /locus_tag="VGE59_03065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9050..9052,aa:Asp,seq:gtc)
     gene            9111..9200
                     /locus_tag="VGE59_03070"
     tRNA            9111..9200
                     /locus_tag="VGE59_03070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9145..9147,aa:Ser,seq:gct)
     gene            9276..>9705
                     /locus_tag="VGE59_03075"
     CDS             9276..>9705
                     /locus_tag="VGE59_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1074653.1"
                     /translation="MTILVTGGAGYIGSHAVKALKAAGHTVIIFDSLENGHEYVLDTL
                     HCRDTFVKGDIRNLEDLEKAFSQYPVDAVMHFAAYALVGESVKEPEKYLRNNTLGTIN
                     LLYAMAKANVHKLIFSSTCAVYGEPQEVPIHEGIPLNPVNP"
BASE COUNT         2678 a         2348 c         2155 g         2524 t
ORIGIN      
        1 gccatatggc acgtcataac aaaagtaggc tgaccattcc acgtttcgcg ggtaaacgta
       61 agatcgctga cagtaacata ttggtttgtg agcgctgatt tcgtatcggc tgccgtaata
      121 gcgccatctc cgttggtatc ccgcactaat tccagtgccg cgcccgttag ctgaagacgt
      181 atacgttgtc cgctagtgag cgtcgcatcc aaagttccac ccgaagtaag aggattaata
      241 acatcatttg attctaaccc agaaaaaagc cagtctgttt tctctcgaac gaaccgttcg
      301 ttcgccacca cttcgctccg ttttttatcg cgtaaacttg cattggtaaa cgtcaaactg
      361 gcagtaatcg taatccccag gattattact actaatccac tatagaggag ggtttcaata
      421 agggtaaatc cacgcatagg agactttcta gctcggtgta ataactcgaa gagcaccatt
      481 gctccggacc gaacagtaaa tcgtgttatt ttcataggca attcctgtca cagattgagg
      541 aaagtttaaa ctggaatacg ccgaaatttg agccggattc gccacatcga gcaccttaaa
      601 ttctgcgtta ggtagctctg ttgctagaaa cgccaaccgg tttactatca ccatatcatt
      661 aatccccgta ttaagatcaa acgtaccttg taccgttggg ctaaccgggt tcgtcgcact
      721 caaaatcaaa aattcaggtt gagtaggaga gttcgcccgg ccgagataaa tattaaattc
      781 atctttaacc gatacgttca taccgtcgta tacgtcagga gcatcatacg tacccaacac
      841 agctatcgcc gcagggtccg ccacatttag aatcatgagt tcagcggtat cgtgcgacgt
      901 tgcgagataa gctcggccat tcaagacagc aatagagtta accgtaccat cgatttcaaa
      961 acttcctaac ttaacaggac tcgtgggagc cgttacatca tagatataaa actcgctttc
     1021 acccgcaatt cgttccatcc ctaagtagag gcgattccca cttaccgtga tactccgtgt
     1081 cttcccatta gaaacatcta cgtcacccac ttttatggga gctgctgggt tcgagatatc
     1141 aataataaca aactccttgg tggaatcagc aacggaacca tacacatagt tgccattctt
     1201 agcaatcgaa gccacattgc taataccgag atcaacacta cttacgagcg tgggggagga
     1261 tgggttttga accgcgaaag tagagaagtc tacctttgtt acattcgttg actggcttga
     1321 gacatatgct ttttggtttt ccacgaatac gtccgttccc gcagcaccac tatcaatatc
     1381 agccgaagct gcgctgaggg gatgcaccca gtctcccgca atatagtttt cctgttgtgt
     1441 gctatgccag ttagtaataa gagtttccag actaatcgtt gcatttcgat tcccacccgg
     1501 tacccacgag accgtgctcg tgactcgctt gctataagca gaaacctctg caatggtaat
     1561 cgagcgagta aacgtatcgc tctgactact cgcaccgctg agtgtccaat taccgtttac
     1621 gaattcaagg ccataggtac cgctcttgag tccgtcccaa ttttgatccc gtataagccg
     1681 ggcaatctcg atgccctctc gggcataatc ggtcgcttgc ttgtttcgtg tcgtttcaag
     1741 tattaagggg cccgatccaa gaactaacgt gaaaagactt aaggtcccca ccaaaaatac
     1801 tcccgaggca atcaggagtt ctattaaagc cgatccagca acacgcttac cagtcaatca
     1861 tcccctcctg attaattgac agtgtaaaat ttcggcccgt tttaggatca acaaagttct
     1921 cactatagga agatttcgag accccaccgg aaacaatggt tggaacccca tcgggagcac
     1981 ggaaacgaaa ttcagttcca gaagcaaata actgttcact acgaggatac acaatgtcat
     2041 gagaagtatc ccgggaagcg taagtggtgc cttcaaacag ggtataattg gaagaattta
     2101 ttttaactcc tgtgtcccgg ccccggaggt ttgcgtacga ttgatcccgg gctgactgga
     2161 ggaggggaat aagggtgtct cgctctctat ttagctgggc actaacgata gaattgagag
     2221 taaacggcaa cgaaacaagg agcaacatcg aaaatatacc aatgacgaca ataagctcga
     2281 taagcgtgaa ccctgagaaa gactgtttca tctcttatcc aaagttttga ctaattgagt
     2341 aaatgggcaa aataatggag attgcgataa aaccaactgc gcctcccatg gtgagcatga
     2401 gcaatggttc gagtaaagac acaaagtttc gcgttgtttc gtcgacttcg gcctcatagt
     2461 aatcactcaa gtacaggagg gttgcgtcca tcgtgcccgc agtttcactt acgtcgagca
     2521 tttgtgaaaa catcaggggc acgtattgag gataattggc aaaataacgt ccaagcggag
     2581 aaccagcctt gacttgctct ccagcagcgg caatcagttg gcgatagaca aggttaggaa
     2641 caatatcgcc cgtaatcaaa agggcttcta caattttgac cccactttta aggagcagtg
     2701 ccattgttcg ggcaataatt gccatttgaa tactcatagt caaaggacca ataatcggca
     2761 aacgaataag gaacatttga aacaagaagc gaaaacgggg aagccgaata agtagaaacc
     2821 ataagccaac caccaccaaa atgcctatta agatataagg ccagtacgct aacataattt
     2881 tgttcgaacc aatcacaatt cgagtcgtaa gaggtaacgg aacttgaaag ctctcaaaga
     2941 taggcaatat ttttggaaaa accgcaaagg taagaatagc cattaataga atcgtggtaa
     3001 tgacaataat aatgggatac atcatcgcgc ttaaaatctt tccacgaagt tcattctttt
     3061 ttttgagctc atccgccaaa aaacctaagt tctccgacaa tgtgccactc gtttccccaa
     3121 tgcgcacaat attaataaag aggctaccaa aaacggactc aaactttttc aagctcgtgg
     3181 ataaaaattg gccattttcg acatcggtaa tcaagactgt gagaactttg cgcatgccgg
     3241 gagaggcggt ttgttgggcc accaaatgca agctctgcaa aattgggatc cctgaccgtg
     3301 acataagcga aatatttcgc gcaaataaaa tttgctcttt aatcggtacc ttcataaaaa
     3361 gagacgagaa cctactcatg gaccacccgt agcacttctt caaccgttgt cttaccttcg
     3421 agcgctttgc ggaacccatc ttgaagcatt gttgtcatgc ctttttgcat cgctagagcc
     3481 acaatctcgt tcgcatttgc ccgacgcata atcgcttcgc ggatagcatc atcaacaagc
     3541 agaacctcat aaataccagt acgaccgatg aatccactct gaccgcattg ctcacaccct
     3601 tgagccgcaa aaaattgccg gttatcaccg agaaaggcaa tggggataac ctccgcaaga
     3661 ctctgatact ctgcttcggt aattgttttg ggaattcggc agtagggaca gagcaagcga
     3721 accaagcgtt ggccaatcgc taagtttacg gttgaagcaa ttaagaaagg ttcgatttta
     3781 aactcggtta atcgtgctaa ggttgtggcg gcgtcatttg tgtgaagcgt cgagagcagc
     3841 aagtgccctg ttaatgccgc gttaacggca atttccgccg tttcttcatc acggatttct
     3901 cccaccataa taatattggg atcttggcgt aaaattgccc ggagtcctgc agcaaacgtc
     3961 agtcctgact gcggattaat ctgaatttgt tcaattccat caatggaata ttcaataggg
     4021 tcttcaatgg taatcactga tacatcgggc gtattgagtg tttttaagat cgtatacaac
     4081 atggttgtct tacccgaacc agtaggtcca gtcgccaaaa tcatgccata cggacgagtc
     4141 atggctcgtt tcactttagc caaatcagac ggagacatat ctagcccatc aatcgttaac
     4201 ttttctgaag gggactgcaa aagacgcagc tcaacgtttt cgccgtagta tgtggggacg
     4261 atcgagacac gaagatcgac agtcccgata tctttgagta gcactttgaa ccggccatct
     4321 tgaggtacct ggtgttcgtc agttcgcaga ccacaaagga ttttggttct cgcaaccaaa
     4381 agagactgaa taattttggg caaagaaaaa acatcataga gaatgccatc gacacgaaag
     4441 cgaactcgga cattctcagg agtgggatca atatgcatgt ccgaagtacg cgttaaactc
     4501 gcgtatgcga taagaaggtc aatcagacgg gcaatcgaaa attcgctcgg ttgccgcgac
     4561 agctcttgtt caataaaagt tttgattgac tcgacagttg cggtcgtctt tgcccgctct
     4621 tcgtcggtca tattgcttaa aacaagtggg atatgcgacg tttttgacac ctcttctgaa
     4681 ggagtagaaa caataggaga agttacttct gccacagggg caggagtatt ttgaggagca
     4741 gatgacacgg gcggaacaga accggaaagt gcgtcgatga taattacccc tccctacgtc
     4801 gttattctta tcttagcata ctccctaaca accctgtcta gcttcaataa aaaaacaggc
     4861 caacgtgata gtgacgcgac ctgtttttct attgcttcgt tagatgacta ctttggcgtg
     4921 gtcaggggca actttgagta attcggtttt cgttagatcc cttaagtaag ccgatagctc
     4981 ttcccgcatt tccggatgtt ggagagcaag ttcaatgttt gtctttatat aatccagctt
     5041 attcccgcaa tcatagtagc gcccgttttg gatagtcatg gcgtatactg gttttccatc
     5101 cgccaccata gcagctacgg catcactgta accatactct ccgtttttca taggttcaat
     5161 cttatcagcg tacgtcatga gttcgggcgt gaacacaaac ccactgacgc aagcatactc
     5221 tgaaccagct tgtccggttc ctggcttctc tatgattctc ctaacacgca gtacaccagg
     5281 ctcaatttca tctccttcgg caaaggcgta acgactacca tgctcagggt tagttgttcg
     5341 gacacagcca agaataggag cgccatattt ttcatacgct gctacaagct gttgggatcg
     5401 agttggagaa gcgacaaaaa aatcatcgcc ccacgttgca acaaacggtt cgtttggcat
     5461 ggctttatag ccataagaga atgctcccat cgtgcctttt atcttcttct gccgaacgta
     5521 aataaaatta gctaagtttg cgatggctcg cacttgctca agttcgttta ctttgccacc
     5581 tttttctaac actgtttcta attcaaacgt gtgatcaaaa tggtcttcaa gcggccgctt
     5641 ttgagcactc gtaacgagga caatatcttt aatgccggcc gacaccacat cttcaactgc
     5701 atactgaatt actggcttat ctaccacagg caacatttct ttgggcattg ccttagtgat
     5761 tgggagaaaa cgggtaccgt agcctgccgc gagaatgatt gccttggtaa ttttcatagg
     5821 ggaatcagtt ctttagattg aggtaacttt gagaatacgg gtttttgact tctcaacccg
     5881 aggaatacgg atcgtcaaaa taccatcttt taagccagcc gaagcagcgt ccggatcgac
     5941 ggcgacaggt agaacgtacg aacgggagaa actaccccag tagcattctt gaacgaagta
     6001 gttctcactg ggtacttgct cattgagaga acgggaacca cgaatcgaaa taatctcatc
     6061 ggtaatagcg acctctagat ttgcctcatt cacccccgca ataggagcaa cgagtacgat
     6121 cgagtcttct gtttgataca catcaacggc aagttgtccc tcgtcctcaa cgtgattcat
     6181 ccagtgttcg tcttggccaa tagttttttg agccatagga tccttcctcc ctccactgtt
     6241 tggcaagcgt cgcgaagcat gcttcactaa acgtatggcc ctaccataca ctggtgcccg
     6301 ttttatcgtc aagctatggg atgctatttt tgaagtagtt tttccaagtg aatgtgtgca
     6361 gtgccacaat gagggcccgt ggctctgcgc ctcatgcctt tctcgctgtg tggcaattag
     6421 actaccaacg tgtccctttt gcgaccgatt gtcaccgctg ggcaagacat gccattcctg
     6481 taaacgaacc tataatctca cggggagccg ctcagcatgg tattatcgcg gcccgataaa
     6541 aacgcttatt ggatcagtaa aatatcactc ccggactgca gcagtgactt ctgtcgagct
     6601 cgcattaagt cagctaatac gcgaacttcc acttccacgc cacgctcccg ttatcgttac
     6661 gtcggtgcca tcaagtccga gcgccttagc aactcgtgga tttaaccaga gcgagctact
     6721 cgctaagagc tgtgccagaa ccactggcac tttgtgttac gtccctcttt taaaacgaac
     6781 caaccgaagt acctctcaaa caaggctcac caggaaagct cgttttgcaa atgttgtagg
     6841 gcaattttct ctcgcaagag caattccttc actaaacaac acaaccatca ttattattga
     6901 cgatgtaata acgacaggtg cgactctcgc tgcctgcgcc accgtactaa aagaggctgg
     6961 agccaaagcc gtgtggggca tcactgtggc gaaagactaa gtattggctc agtgcttcca
     7021 ggcaagatgg taagcagccc atccttaaca agcccttcaa tcagctcctc aagcttatac
     7081 ggcatatcag acggaagaat atctcccact tgggagaggg aagcttgatg gttttcacac
     7141 gaagtaaggt aggcaatgat acgccctcgg aacgaacgtc tcgacccaac gaaagacgat
     7201 tgcttcggtc gagcaaatgg tttacttccg agcagaggaa gggtccaacg agggataaag
     7261 gctaactctt ctaagagaga ctgctgttgt tcaagaggtg tacgacgctt tcgtacacat
     7321 gtcatgagat ccattatcgc actatgccac gcccgactac acgcagaagg aacatacgac
     7381 tgggcaaaag tagcaacctc tttcacggat ggtttttcat aaccccagaa gagtaattca
     7441 taaaaacgct caatattcgt gtcgaccatc gcaactggct cgttataaga aaaagctgct
     7501 atcgcaccag ccgtgtaagg tccgatccca gggatagatt taagtgtgtc tataccatga
     7561 gggacatcat cactataaag agtgtgaatc gtctgagccg ctcgatgtaa tagtactgcc
     7621 cggcggttat agccaagacc agaccaaaga gcgatgacat cagagacaga agccgacgcg
     7681 agactttcga cagtcgggaa tcgttttaaa aatgcagtgt attttggaat aacgcgttca
     7741 acttgagtct gttgaagcat gaactccgac accataaccc catagcccgg gtcgacacct
     7801 aaatcagcca catttcgcca ggggagagag tgacgaccat ttttttcgta ccaacgtaaa
     7861 acactggctt gaagcggggt aaaagaattt gtggacatag aacacccata ccataccttt
     7921 acgttttcct taaaatttgg tatagttatt ctcgtattta agaatacgta aagagcctgt
     7981 agctcagtgg atagagcgtc tggttgcggt ccagaaggtc gagggttcga ctccccccag
     8041 gctcgccaac aagaagtccc ggtaatgccg ggattttttg ttggcgtgga tttaagagag
     8101 gaggagaacc ctagattcga tagacaggaa cacacgcaga agctcacttc gagcatgtga
     8161 cgaagctata cgaagtacac cccctaggct cgccatgaaa accgtggggg attcgtatct
     8221 ctacccttct ccgtctctcc gaaccattca ttgtgcgacg ccatgtaata gatatgcttg
     8281 ttcgaacgta acgcagtgct acaatacata tatgaaagct ccttcgcgta cgtcttttaa
     8341 accaatttcc tggcgcgctc ccgaatatct ctttcgccgt aaaactctgc tgtggtatac
     8401 gcatgtctcg gtatttttct ttctcgtact tgtacttctt ttttggattc aaaactggtt
     8461 tggtgtggtt attctcgtcc ttctcttttg gctttttatg agtaaatccg aagaaaaacc
     8521 aaagaatgtt acgtacacca ttgaccacca tggcattact attggtgaca gaacccttac
     8581 ttttagtgaa atcgaaagtt ttaccgccga gattgtccac cgcacaccga tcattaccct
     8641 caatcttacg tattcttttg ctctgccgat tacgctcatt gtgaagcacg atgtaaaaga
     8701 tgaagtaata gaccacctga gtcagtacat ccccttgtca aatgactttt cttttatgcg
     8761 atggctttct caccgtcttc actactagca cctctcacga ctagaacgga aggctctctt
     8821 ctgttataat acaggggtat ttttggagag gtcgcataga ggcctagtgc ggcggtcttg
     8881 aaaaccgttg agcgaaagct ccgtgggttc gaatcccacc ctctccgcca gaagcgaatt
     8941 tctagggatg gatgatgcat agaaattggc tcctggcatc aggataaccc agcaaatggt
     9001 gacaggattt gctggaccgg tagcttagtt tggcttaaag cgcctctttg tcgaagagga
     9061 gatcacgggt tcgaatcccg ttcggtccgc cagtacaaaa gaatcataat ggagaggtgc
     9121 cagagtggcc gattggggca tcctgctaag atgttgtacc cgtaagggta ccgagggttc
     9181 gaatccctcc ctctccgcca tgcaaaaatc tgcccaacgg cagatttttg cgtttcacct
     9241 tggttttgct agactatgca aaaaaggaac ttgctatgac catactcgta actggtggag
     9301 ctggatatat tggcagtcat gcggtcaaag ccttaaaagc cgctggtcac acggttatta
     9361 tttttgactc cctcgaaaat ggtcatgaat acgtgctcga tacattgcac tgtcgagata
     9421 cctttgtgaa aggagacatt cgcaatttag aggatctgga aaaggctttt tctcaatacc
     9481 ctgtcgatgc ggtcatgcac tttgccgcct atgcactcgt gggagaatct gtaaaagagc
     9541 ccgagaaata tcttcgcaat aacacccttg gcacgatcaa ccttctctat gccatggcaa
     9601 aagcaaatgt tcataagctt attttttcct ctacctgcgc cgtatatggg gaaccccaag
     9661 aagtccctat tcatgaaggc atccctctca acccagttaa ccctt
//
LOCUS       DASWYB010000052         5373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_83022, whole genome shotgun sequence.
ACCESSION   DASWYB010000052 DASWYB010000000
VERSION     DASWYB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83022"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VGE59_03080"
     CDS             <1..272
                     /locus_tag="VGE59_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY1074654.1"
                     /translation="LVMKKITLYGTPICVHCTALKAFLKEKDVEYTYYNVLDDQSKAE
                     EMIDLTHQTAVPVLVIADKEEKSDPQILIGFNESMIPEYELALGL"
     gene            334..720
                     /locus_tag="VGE59_03085"
     CDS             334..720
                     /locus_tag="VGE59_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074655.1"
                     /translation="MKVLVIRGDTTTTAPFLGLRNGKPIQVIPITRLEEVEYHLRLRN
                     TAIRVVIDPSAELFTPTGETEARVDGILQAVRAHRKRGIFFIERYERTDERVKALMEQ
                     HPGVGYISVHPTRFLAEVGEQLGLHT"
     gene            complement(833..958)
                     /locus_tag="VGE59_03090"
     CDS             complement(833..958)
                     /locus_tag="VGE59_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074656.1"
                     /translation="MIGKIFGLISGKKGKTTKKASVAAVKKAKKKAAKKEKCEFC"
     gene            1053..3749
                     /locus_tag="VGE59_03095"
     CDS             1053..3749
                     /locus_tag="VGE59_03095"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEY1074657.1"
                     /translation="MNNHSPSPLVYQLENKDVLESLKTTDAGLSVSEAKERLVHYGAN
                     RLTSKRKVSPWKVFFAQFSNSLTITLIVAALLIMFVWYFGERDSSDLIEASLILAIIV
                     LIAGLGFFQEYKAERAIESLKKMLAFQAKVLRGGKEIQIEVAHLVPGDIVILEEGEKI
                     PADIRLLTVASFQINEAPLTGESAPVSKLATPLSGSLQIADQKNMAFSGTVVTQGRAT
                     GVVVATGDATEIGKIAQSVADVEETETPIQKRLDTVGRILGYIVLGIAIVVFIFIMFF
                     AAEFAHQTLLQRVIHSFIAAIALAVAAIPEGLPAVVTISLALGTQRMLKRNALVRKLN
                     SIETLGSTNVICADKTGTLTKGEMTVRELYADGVHYSLTGTGYERMGYFMRDGKKVAP
                     DDLSLLLASGYLCNNAKLTSRSILGDPTEAALLVSSGKSHVAPVAKRLDEVPFSSERK
                     MMSVLVREKGRVRVYSKGAPEVLLSHCTSILKGGKVVALTTADKKKVLEATEDMSQRA
                     LRTLGFAYREAESGEKEIEQKLIFIGLQGMIDPPRSEIKSLIRQCADSGIRVIMITGD
                     HAATAKAVAHEVGIPGEVSVGNELDALSPSDFQKVVSQTNIFARVSPSFKMQIVEALK
                     KQGHIVAMTGDGVNDAPALKMSDIGIAMGKTGTDVAKEASDMVLLDDKFSTIIAAIEE
                     GRGVFDNIRKFVNYLLSCNIGEVIVVFVALLIFKEIPFTATMLLWINVITDGLPAVAL
                     GMDPAEKGIMRYSPKKFQGEIIPARLWGEMIIFGILLTVGVLGIYWFNLPEGVEEARA
                     AAFMAIVIFELVRLFAIRVEYKTSFFSNGWLLVAAFFSLALQCLLVYVPALARLFDIR
                     GIDWHDWVLIALISLVLWYAFEALRWILNHFYPERLEESPQH"
     gene            3786..4610
                     /locus_tag="VGE59_03100"
     CDS             3786..4610
                     /locus_tag="VGE59_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074658.1"
                     /translation="MKHFRGAVLILFFMAVFALAMPAFAAHTPLVADGSDIEAELSSL
                     GLRLVLMTLAGVFVLLLASMAIHKPTELLKRSLFWGITIAVVTTTFVLIFFSVTLNTN
                     SWSKGPIHWHADYQVYACGQQLDLVDPVGLGNKIGTPSRHEHNDNRLHYEGVVLSSGN
                     AKLGSFMEVLGAELTNDSLVFPTNTETVHYHTGDTCSDGTVGEVQVFLYRVGAGNVYT
                     QTKLEHPEDYIIRSESAVPPGDCLVIEFGAPKEMTDHICRSYEVADQIGKIRKATQ"
     gene            4607..5308
                     /locus_tag="VGE59_03105"
     CDS             4607..5308
                     /locus_tag="VGE59_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074659.1"
                     /translation="MIDLPTLSVVVTSALIDSINPCAIGVLILMISVVLSGKGKLSRL
                     VFLGSLYIFSVFAVYLLAGLGLVYFLAAVPLWVTEYLSLAVGGLVIFAGLLEIKDFYW
                     YGRGFSLEIPVFFSKKIHKFAKRATVPGVILLGAFVSGVELPCTGAPYLAIITLLSLN
                     FNAQAFFMLVLYNIIFVAPLIVILAMVALGVKVSHIKRWKQDNKHYMRLGIGLLLICL
                     GWLLMLIANGTINFG"
BASE COUNT         1301 a         1170 c         1308 g         1594 t
ORIGIN      
        1 agcttgtgat gaaaaaaatt acgctgtatg gtacccctat ttgtgttcac tgcactgcgc
       61 ttaaagcatt tcttaaagaa aaagatgttg aatacaccta ctacaacgtc ctggatgatc
      121 agtccaaagc cgaagagatg attgatttaa cccatcagac agcggtaccg gttttagtaa
      181 ttgctgataa agaagaaaaa agcgatccgc aaatcctgat tggttttaat gaatccatga
      241 ttcccgaata cgaattagcg cttgggcttt agcttgcgtt cgtttgtctg catgttacga
      301 tagagaaggt ctctgacgta catggagaac gaaatgaagg tgctggtcat acggggggat
      361 acgacaacaa cggccccctt cctaggactc agaaatggga aacccattca ggttatccct
      421 attacccgct tggaggaggt ggagtatcac cttcgtctga ggaatacggc tatacgggtc
      481 gttattgacc cgagcgctga acttttcacc cctacgggcg aaacagaagc tcgggtcgat
      541 gggattctcc aggccgttcg tgcccatcga aagcgcggaa ttttcttcat cgagcgttac
      601 gagcgtacag atgagcgcgt gaaggctctg atggagcagc atccgggagt tgggtacatt
      661 tccgtacatc ccacgcgatt tttagccgaa gtaggcgaac agctaggtct gcatacctag
      721 gcctttctcc tacagggaaa tcgcacgctt tacggaggga ggcgcaagcc attccctcct
      781 tttcttattc taaaaaacgc accggagtgc gttttttaga ctcgaggaga gattagcaga
      841 attcgcactt ctcttttttt gcggctttct tcttggcttt tttcactgcc gcgacactcg
      901 cctttttcgt agttttcccc tttttgccgg agatgaggcc aaagattttt ccaatcatgt
      961 ttccatgtta cggaactagt gaagcgctgt caaacagccg taaaaaatca cccgtttgca
     1021 tggcccgacc aagctaccgt atagtggaaa taatgaataa tcactcccct tctcctcttg
     1081 tgtaccagct tgaaaataaa gatgtccttg aatctctcaa gacaactgat gcgggtttat
     1141 ctgtctccga agctaaggaa cggttggtcc attatggagc caatcgcctg acatcgaaac
     1201 gaaaagtttc accgtggaag gtgttttttg cgcagttttc aaactcgctc accattacgc
     1261 ttattgttgc ggccttatta attatgtttg tgtggtactt tggcgaacgt gattcttcgg
     1321 atttaattga agccagcttg attcttgcca taattgttct cattgctggt ttaggttttt
     1381 ttcaagagta taaagcggaa cgagccattg agtcgctcaa aaaaatgctt gccttccaag
     1441 ccaaagtatt gcgtggtggt aaagaaattc agattgaggt agcccacctt gttccgggcg
     1501 atattgttat tctcgaagaa ggcgaaaaaa ttcctgccga tatccgtctc ttaactgtgg
     1561 ctagttttca aattaacgaa gctcctttaa ccggcgagtc cgcaccggtt tcaaaattgg
     1621 ctactcccct ctctggctct ctgcagattg ctgatcaaaa aaacatggct ttctccggta
     1681 cggtggtaac gcaaggtaga gccactggcg tggttgtcgc cacgggtgac gcaacggaga
     1741 ttggaaaaat tgctcagtcg gttgctgatg ttgaagaaac cgaaacgccg attcagaaga
     1801 ggctcgatac cgtcggacga attttaggtt atatcgttct tggcattgcc attgtggtct
     1861 ttatttttat tatgttcttt gcggcggaat ttgcgcacca aacgttactt caacgcgtca
     1921 ttcattcgtt tattgcagcg attgccttag cagtagctgc aattcctgaa gggctaccag
     1981 cagtcgttac gatctcactg gcgcttggca cgcagcgaat gctgaagcgc aatgccctgg
     2041 ttcgtaaact aaactcaatt gaaaccttag gctcgacaaa tgttatttgt gccgataaaa
     2101 cgggaaccct tacaaaaggt gaaatgacag tccgtgagtt gtatgctgac ggtgtgcact
     2161 atagccttac tggtacgggc tatgagcgta tggggtactt tatgcgtgat ggaaaaaaag
     2221 tcgctcccga tgatctctct cttttgttag cttcagggta tctctgtaac aatgccaaac
     2281 tcaccagccg ttcaatcttg ggagacccta cggaagcggc actgcttgta agtagtggta
     2341 aatctcatgt ggcgcccgtc gcaaaacgtc ttgacgaagt cccgtttagc tcagagcgga
     2401 agatgatgtc ggtccttgta cgtgaaaaag ggcgggtacg cgtgtactca aaaggggctc
     2461 ctgaagtctt actctcgcat tgcacgagta tattgaaggg tggcaaagta gttgccttaa
     2521 cgacagctga taaaaagaaa gttctcgaag caacagaaga tatgtcacag cgagcgttac
     2581 ggacgcttgg gtttgcttac cgtgaggcgg aaagcggcga aaaagagatt gaacaaaaac
     2641 ttatttttat tggtttgcaa ggaatgatcg atcctccccg ctctgaaatt aaatcgctca
     2701 tacgtcagtg cgcagattct gggattcgtg tcattatgat taccggggat catgctgcga
     2761 cggctaaggc tgttgctcac gaagtaggta ttcccggaga agtctcggtg ggtaatgagc
     2821 tcgatgccct ttctccgagt gattttcaaa aagttgtgag tcagacaaat atttttgccc
     2881 gggtgagccc ttcgttcaaa atgcagatcg tcgaagcact aaagaaacaa ggacatattg
     2941 tggctatgac gggtgatggg gtgaatgatg cgcccgctct caaaatgtcg gatattggta
     3001 ttgcgatggg aaaaacaggt actgacgtcg ccaaagaagc atcggatatg gtgttgctcg
     3061 acgacaagtt ctcgacgatt atcgctgcga tcgaagaagg acgaggcgta tttgataata
     3121 tccgtaaatt cgtaaactat cttttgtcgt gtaatatcgg cgaagttatt gttgtctttg
     3181 ttgccctttt aatttttaaa gaaattcctt ttacggcgac catgctgctt tggattaatg
     3241 ttattaccga cggactaccg gcagtggctc tcgggatgga tccagcggaa aaagggatta
     3301 tgcgctactc tcctaaaaag tttcagggag agattattcc cgctcgactt tggggtgaaa
     3361 tgattatttt tggtatttta ctcactgtcg gtgtgctcgg catctactgg tttaatcttc
     3421 ctgaaggggt ggaagaagct cgagcagcag cctttatggc gattgttatt ttcgaattgg
     3481 tccgtctctt tgctattcgg gtagagtata aaacatcgtt cttctccaat ggctggttgc
     3541 tggtggccgc attcttctca ctagcgctcc agtgcttgct ggtctatgtg cctgcgcttg
     3601 cccggttgtt cgacatccgt gggattgatt ggcatgattg ggtgttgatc gccctgatta
     3661 gtcttgtttt gtggtacgcg ttcgaagctc tacgttggat acttaaccac ttttaccctg
     3721 agcgtctcga ggagtcaccg caacactaat tgcgccgctt cctagccatg tggtaggctg
     3781 gttatatgaa acactttcga ggggcagttc ttatactctt ttttatggct gtttttgctc
     3841 ttgccatgcc agcgtttgcc gcacacacac cactcgtggc ggatggctca gatattgaag
     3901 cggagttatc ttccctcggt ctgcgccttg ttttaatgac actggccgga gtctttgtcc
     3961 tccttctcgc ttcaatggcg attcacaaac cgaccgaact tttaaaacgt tctttatttt
     4021 ggggcattac cattgctgtg gtaacaacaa cctttgttct tattttcttc agcgtgactt
     4081 taaataccaa ttcttggagt aaagggccaa tccactggca cgcggattac caagtctatg
     4141 cttgtggtca acagctcgat ttagttgatc ccgttggact gggtaataaa attggaacgc
     4201 cgagtcgcca tgaacacaac gataatcgtt tgcactatga aggcgttgta ctttcgtctg
     4261 gtaacgcgaa acttggcagt tttatggaag tgctcggagc agaattaacg aatgatagct
     4321 tggtttttcc aaccaatacc gaaacagtgc attaccacac gggtgatacg tgctccgatg
     4381 gtacagtcgg tgaagttcaa gttttcctct atcgtgttgg agccggaaac gtttatacac
     4441 aaacaaaact ggaacatccg gaagactata ttatacgttc agaatcggct gttcctccag
     4501 gtgactgtct cgtaattgaa tttggtgcac ctaaagagat gacggaccat atctgtagga
     4561 gctatgaagt agccgatcaa attggcaaga ttcgaaaggc gacgcaatga ttgacctgcc
     4621 aacactttct gttgttgtta cgtccgcgtt aattgattcg attaacccgt gtgctattgg
     4681 tgtgttaatt ctcatgatct cggttgtgct aagcgggaaa ggaaaactgt ctcgattagt
     4741 gtttctgggg tcactctata ttttctcagt ttttgcagtc taccttctgg ccgggcttgg
     4801 actcgtctac ttcctggccg ctgttcccct ttgggtaact gagtatcttt cactagcagt
     4861 cggtggtttg gttatttttg ctggattact cgagattaaa gatttctact ggtatggccg
     4921 tggtttttcc ttagaaattc cggtcttttt ctcaaaaaag attcataaat ttgcaaaacg
     4981 tgcgaccgta cctggcgtta ttctcttagg tgcgtttgtt tctggtgttg agctcccctg
     5041 tacaggcgcc ccgtacttgg cgattattac gctactctca ctcaacttca atgctcaggc
     5101 cttctttatg ctggtgcttt ataacattat ctttgtcgcc ccgctcattg tgattctcgc
     5161 aatggtagcg cttggcgtga aagtgagcca cattaagcga tggaagcaag ataataaaca
     5221 ctacatgcgg ttggggattg gtttgctctt aatctgttta ggttggttat tgatgttgat
     5281 tgctaatggc accattaatt ttggatgata tatgacccgt catcttgtaa aacgctctgg
     5341 aatacaagaa cccttcgatc ctcgtaaagt gta
//
LOCUS       DASWYB010000053         6508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_30269, whole genome shotgun sequence.
ACCESSION   DASWYB010000053 DASWYB010000000
VERSION     DASWYB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30269"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VGE59_03110"
     CDS             <1..331
                     /locus_tag="VGE59_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074660.1"
                     /translation="VHGDWKHSQLIKCNETYYIFDFGRSFWGPSILDYGYYFKDDAEG
                     GLEYFSTNKTLLLKAKLVALIMQLEWFVRCRENYISYDYNKEISETQSKMEAVTALLN
                     KEESTLC"
     gene            325..792
                     /locus_tag="VGE59_03115"
     CDS             325..792
                     /locus_tag="VGE59_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY1074661.1"
                     /translation="MLDIEKTIREYLPQVVHMSLATCRDNHPWVCEVHFAYDDELNLY
                     FRSTLNRRHSQELLENPCVSGNIITQHFLNQKVRGVYFEGTAKLIESDEELNIAFNAI
                     KERLHLDEEILVDAHKEDGHKFFKLSVTNFYLFDSYTSNPSQKYQLEWSKRAL"
     gene            complement(789..1811)
                     /locus_tag="VGE59_03120"
     CDS             complement(789..1811)
                     /locus_tag="VGE59_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074662.1"
                     /translation="MGEMHESHTPEEAEKWEFNSTLTRHLPRDNKFEITQEGPEAAQE
                     AARELAEHIRTLPDRSVITIVPSIVFRNKETRRHYEETLATELAEDSDIEFLPVKSED
                     GTPNSEYRETFMQTVEESDKKIIVTGIQPQTLLGYRHDHETLPDNEALSGFVYLLGEL
                     QGSEELVGKLWMCDASEFPALAQEIESRLPGVDTTKLTPGEIALTPEDFAKNYIQFLH
                     RFYEIAKKHFPTRPFYAVNITHDFYSDFTTMAAMGKPINLQSLQEFGGSFRDFLESSE
                     ISFDGESVTTTYRGETITAPKTFKEIIEELSIASESRKEMWAEHFPAPHEQLDSSSEE
                     TESAQS"
     gene            complement(1813..2913)
                     /locus_tag="VGE59_03125"
     CDS             complement(1813..2913)
                     /locus_tag="VGE59_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY1074663.1"
                     /translation="MNRDFSQLRADQFHPLFAFEGIGYLFTEQFLAQYSKYEIVVVYE
                     NNFFRSFISKVGMEKTLQEGYDLFANEEKFAENCKAWEAYLVRAKKVIETYQTPEDDL
                     TVEDWQLVTTTLRECWVYHDKTEFIFTDTAFLAQETNPVIAKSLKRLGEIKFPSSEVL
                     HMLGLNVFYRVAEKIADQFDITHQDIKFFSTKEVEELFSGKKPDMNRIARRKNAYVLL
                     GFDGVVTEPSAEVTALIVDEFRVKASDIKTLRGITASKGEVEGNVRVILADYGSDFRA
                     VDEYVKAMQTGEILVTENTTVQFWPVLQKASAIITNQGGLNSHAAIVAREMKIPCIVG
                     VANATEILKTGERVSLEALGREGVITRVEETA"
     gene            complement(3045..4202)
                     /locus_tag="VGE59_03130"
     CDS             complement(3045..4202)
                     /locus_tag="VGE59_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074664.1"
                     /translation="MREKDWQYPLLHRLTELYGEGKPMSNVLLIACQHLLGTQYEMVT
                     SLYRLGLKPSNCILIGKNYSANREIVRQLHQDGCKVAPFSESFDPDQPFDTWFGRHVR
                     DYLRKQLWWRGMWPYRKIVVLDDGGLLHEVCNTLPSWYHRAMAGVEQTTSGDTKIKKA
                     GMKYPVRSIARTHYKQYDEAPYIAEHAYERILSHMKTRKKGASKVLVLGLGTIGRNVA
                     GRFWRDGHNVYAADATPGKTLAYGACQLLRNKGCVLSVSDAYSRLKEFDVIVGATGTS
                     LFDESVVPTLHPEVSLVSVSSSDREFPAPYFRRGVAGSVHEDYYCGGRCLVNGGFPIT
                     FGGNYHEVAPEHIELTVATLMASVLDAVAPEQQYFQYVIHQLAGMVESGPT"
     gene            4354..4662
                     /locus_tag="VGE59_03135"
     CDS             4354..4662
                     /locus_tag="VGE59_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074665.1"
                     /translation="MKQGSFKNQTKVWMAISGACGFTFFCIANFTHLLHGLTLLAAGI
                     QLAINTFVFFSWKRGYQEAQRPLQKLVALTGTIFPVVMASITICRVIIPAFVRLLAAP
                     "
     gene            4822..5253
                     /locus_tag="VGE59_03140"
     CDS             4822..5253
                     /locus_tag="VGE59_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074666.1"
                     /translation="MTESEIFERIKPTKTLFELLMALRKIGSITGSQTVSYERLIRPV
                     IEAVKTGNVSRITRTGELKSKVAWLAQQTHRPLVDWYLIALLSEESLPVASPTSLMEW
                     FTCATSAAEAGLTEGQRYGEIPLVRQVEDLRDSIIALTAEL"
     gene            5532..6086
                     /locus_tag="VGE59_03145"
     CDS             5532..6086
                     /locus_tag="VGE59_03145"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY1074667.1"
                     /translation="MLGFPVPCGEIEGISLVLVPTTREHAHYFVKWVNDPETPHYGGR
                     IRGCTEEGEERWYDDKRQDDTFILWTIMLGEEPIGLSWVTGMDQRLRTGYTGIHIGRK
                     DLWGKGIATKVMKARSTHLFLEHNFQALFSQIFMPNVGSRTAAERAGYVQWGIRPYAG
                     YHNGTYLDLWEGVLTRERWQQLSQ"
     gene            6104..>6508
                     /locus_tag="VGE59_03150"
     CDS             6104..>6508
                     /locus_tag="VGE59_03150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1074668.1"
                     /translation="MFELAVDEDIRLILRDPNEAEALFSLIDRNRGHLRPWFNWVDTT
                     QSVDTTRKYLEMCVQGFEAGTLVDLGIRYRDEWVGSLSLHAIEKASRKAEIGYWVAQD
                     HQGKGIVTRAVRALIEYGFGSLNLNRVAILCNV"
BASE COUNT         1719 a         1644 c         1469 g         1676 t
ORIGIN      
        1 cgttcatggc gactggaaac actcacagct tatcaagtgt aacgagacct attatatctt
       61 tgactttggc cgatccttct ggggaccatc tatcttagac tatggttact actttaaaga
      121 cgatgcagaa ggtgggcttg agtacttttc tactaataag acccttctgc taaaagcgaa
      181 actcgttgct ctaattatgc aactcgaatg gtttgtccgt tgccgggaga actatatctc
      241 ctatgattac aacaaagaga tctccgagac tcaatcgaag atggaagccg tcacagcgct
      301 tctgaacaaa gaagaaagca ccctatgcta gacatagaga aaacaatccg cgaatatctg
      361 cctcaagttg ttcacatgtc actggcaacg tgccgagaca atcacccatg ggtatgcgaa
      421 gtacactttg cctatgatga cgagctcaat ctgtattttc gctcaaccct aaaccgccga
      481 cacagccaag agttattaga gaacccatgt gtatctggaa atataatcac ccagcacttc
      541 ctgaatcaaa aagttcgtgg tgtgtacttt gagggtaccg ctaaattgat tgaatcagac
      601 gaggagttaa atatcgcctt caatgctata aaagaacgcc tacatctaga tgaagaaatt
      661 ttggtagacg cacataaaga agatggtcat aaattcttta aactatcggt caccaacttt
      721 tacctcttcg acagttacac ctcgaaccca agccaaaagt accaattaga gtggagtaaa
      781 agagcccttt aggattgggc cgattcagtc tcttcgctcg aagaatctaa ttgctcgtgt
      841 ggagctggaa agtgctcggc ccacatttcc tttctcgact ccgaagcaat tgataattcc
      901 tcgataatct ctttgaatgt tttgggggcg gtgatcgttt cacctcggta cgttgtggta
      961 accgactccc cgtcaaagga gatctcggaa gactctagaa agtcgcggaa cgaaccacca
     1021 aattcttgta agctttgaag gttaatcggc tttcccattg ccgccatggt cgtaaaatca
     1081 ctgtagaaat catgcgtaat atttactgcg taaaatggcc gtgttggaaa gtgttttttt
     1141 gcaatctcgt aaaaacggtg caaaaactga atatagtttt ttgcaaaatc ttcgggcgtt
     1201 aaggcaattt cacctggcgt gagctttgtt gtgtcgactc cgggaagtcg agattctatc
     1261 tcttgagcca gggcagggaa ttcgcttgca tcgcacatcc acagtttacc aactaattct
     1321 tcgcttcctt gtaactcgcc aaggagataa acaaaaccgg aaagagcctc gttatcgggg
     1381 agcgtttcgt gatcatgacg gtagccaaga agcgtttgag gctgaatacc agtcacaata
     1441 attttcttat cgctctcttc taccgtctgc ataaatgttt cacgatattc tgagttgggc
     1501 gtaccgtctt cacttttcac aggtaaaaat tcaatgtctg aatcctcggc caactcagtg
     1561 gcgagcgttt cctcgtagtg ccttctcgtt tctttgtttc taaatacaat tgagggaact
     1621 atggtaataa cagaacgatc aggcaaagta cgaatgtgct ccgctaattc tcgggccgct
     1681 tcttgtgccg cctcaggtcc ttcttgagtt atttcaaatt tattatctcg aggaagatga
     1741 cgggtgagag tgctgttaaa ctcccacttt tcggcttctt caggagtatg agattcgtgc
     1801 atttcgccca tgttatgccg tctcctcaac ccgggtaata accccttcgc gacctaatgc
     1861 ctcaagagag actcgttcgc ctgtttttaa aatctcggtt gcattggcca ctccaacaat
     1921 acaagggatt ttcatctcgc gcgccacgat tgccgcatgt gagttcagcc caccttgatt
     1981 ggtgataatc gccgaagctt tctggagaac tggccaaaat tggaccgttg tattttcggt
     2041 aactaaaatt tcacccgtct gcatagcctt gacatactcg tccacagctc gaaaatcaga
     2101 tccataatcc gctaaaataa ctcggacatt tccttcgact tcacccttac tcgccgtaat
     2161 accacgtaag gttttgatat ctgatgcctt aacccggaat tcatcaacaa tgagagccgt
     2221 aacttctgca cttggctcag tgacaactcc atcaaaacca agcaatacat atgcattttt
     2281 acgacgagcg atacggttca tatctggttt tttacccgaa aacaactctt ccacttcttt
     2341 ggtcgagaaa aatttaatat cctggtgcgt aatatcaaac tgatccgcaa tcttttcagc
     2401 cacccggtaa aatacgttca aaccgagcat atgcaatact tcactggaag ggaacttaat
     2461 ttcaccgagt cgtttgagac tcttggcaat aactgggttt gtttcttgag caagaaaagc
     2521 cgtatcggta aagataaact cagttttatc gtgatagacc cagcactctc taagggtggt
     2581 agtcacaagc tgccaatcct ctaccgtaag atcgtcttca ggagtttggt acgtttcgat
     2641 gactttctta gcccggacta aatatgcttc ccaagcctta caattctcgg caaatttttc
     2701 ttcattggca aaaagatcat acccctcttg aagtgttttc tccataccta ctttcgaaat
     2761 gaaactgcgg aaaaagttat tttcatacac caccacaatt tcgtatttgg aatattgggc
     2821 caagaattgc tctgtaaaca agtaaccgat tccttcaaaa gcaaagagag gatgaaactg
     2881 atcggcacgt agctgactaa aatctctatt cattgagttt atgtctccat ttctatagca
     2941 aaccaggtta cggtgaataa tataaactat atcatgttct tgaagcgatt caatcaaata
     3001 gagtagatca ctttattcct ataatcttta taaaaaaagc ggccctaggt agggccgctc
     3061 tccaccatac ccgcaagttg gtggataacg tactgaaagt actgctgctc gggagccact
     3121 gcatcgagta ccgaagccat gagcgtagcg acggtcagct cgatatgttc cggagctacc
     3181 tcgtggtagt tgccgccaaa ggtaatcggg aagcccccgt ttaccagaca gcgaccaccg
     3241 caatagtagt cctcatgcac actgcccgca actccccggc ggaagtacgg cgccggaaac
     3301 tctcggtctg acgatgagac actcaccaga gaaacctctg gatgaagcgt cgggacaacg
     3361 ctttcgtcaa agagtgaggt tccggttgct cccacgataa catcgaactc cttcagtctg
     3421 gagtaagcat ccgaaaccga cagcacgcat cctttattcc gcagaagctg acacgccccg
     3481 taagcgagcg tcttgcctgg cgtagcgtcc gcggcgtaga cattgtgtcc atctcgccag
     3541 aaacggccgg ccacgttacg gccgatagtc ccaagcccca agaccagaac cttcgaagca
     3601 cctttcttgc gcgtcttcat gtgagaaaga attcgctcat atgcatgttc tgcgatatag
     3661 ggcgcttcgt cgtactgctt gtagtgggtc cgggcgatcg accgcacggg atacttcatt
     3721 ccggccttct tgattttcgt atcccccgat gtcgtctgtt ctacgcccgc catagcacgg
     3781 tgataccagc tcggcagcgt attacaaact tcatgaagaa gacctccatc gtcaagcacc
     3841 acaatctttc gatacggcca catgccacgc caccagagct gctttcgcag gtaatcgcga
     3901 acatgtctcc cgaaccaagt atcgaacggc tgatccgggt cgaaactctc gctaaaggga
     3961 gcaaccttgc acccatcctg gtggagctgc cggacaatct cgcggttggc cgagtagttt
     4021 ttaccaatca ggatacagtt gctcggctta agcccaagcc ggtagagcga cgtaaccatc
     4081 tcgtactgcg tgccaagtaa gtgctggcac gcaatgagaa gtacgttact cattggtttc
     4141 ccttctccat acagctccgt gagccgatgc agcaaagggt actgccagtc tttttctcgc
     4201 atacacgtct ccgttaccca ataccgataa ctgacgtaat atatatcata aaatgtttaa
     4261 aaaatcaacc catcacagtt cattacttga ctagacgaag aaatataagt atactctttt
     4321 tatctgcttc tattcgacct cgaggctact gacttgaaac aagggagctt caaaaaccag
     4381 acaaaagtct ggatggcgat ctcgggagcg tgcggtttta cgttcttttg cattgccaat
     4441 tttacgcacc tgttgcacgg cctcacgttg ctcgctgcag gaattcaact ggccattaac
     4501 acgtttgtgt ttttcagttg gaaacgagga tatcaggagg cgcaacggcc gcttcaaaag
     4561 ctcgttgcac tcactgggac aatctttcct gttgttatgg ctagtattac catctgccgg
     4621 gtcataatcc cggccttcgt ccgcctcctt gccgcaccct agcggccggg aggcgtttcg
     4681 ctatacaatt gttttttagt ttttccttgt tctcagactc tccccaacta aacaatatta
     4741 caggttaata ggttgacaaa cctccaattt tatagtatat ttcttctagt tcttcgacgg
     4801 gagagaagga aaaggttcga aatgacagaa tccgaaatat tcgagagaat caaacccaca
     4861 aagactctct ttgagctcct gatggcgctc cgaaagattg ggtcaatcac agggtcacaa
     4921 accgtctcct atgaaagatt gatccgaccg gtcattgagg ccgtaaagac cggcaatgtt
     4981 agccggataa cgcgcactgg ggagctcaag agtaaggtgg cgtggctagc gcaacagacc
     5041 caccggccgc tcgtcgactg gtacctgatc gccctccttt ccgaggagtc tctgccggta
     5101 gcttctccaa ccagcctgat ggagtggttc acctgcgcaa cctcggccgc agaggcaggc
     5161 ctcacggaag gccaacgcta cggagaaatc cccctggttc gacaggttga agacctccgc
     5221 gactccatta tcgcgctcac ggccgagcta taagctcact tcactcacgg cctcttgttt
     5281 ctccttcggg aaacaagagg ctgtttgcat tttattaaag ttcgaagaga tatacatatt
     5341 agaccttgac gaagcaccga ataagaaata tactatacac atatgaaaca cactcatatt
     5401 tttatgattt caaatcgcgc cggagatacg ccacgtaacc cgtaggttgg tttttcgtat
     5461 acgatcagta tgcaagagac gagcctacgg gctcgtctct ttgtgttcca aagagaaacc
     5521 ggagcgcacg tatgctagga ttcccagttc cctgcggaga aatcgaagga atatcactgg
     5581 tacttgtacc taccacccga gaacatgccc attactttgt aaaatgggtt aacgatcccg
     5641 agacacctca ttacggaggt cgaatccggg gatgcaccga ggagggagaa gaacgatggt
     5701 acgacgacaa gcgccaagat gacacgttca ttctttggac tattatgctc ggcgaggaac
     5761 ccattggttt gtcttgggtg acaggcatgg accagcgact acggacggga tacaccggta
     5821 tccatattgg tcgcaaagac ctctggggca aaggcattgc gaccaaggtt atgaaggcac
     5881 ggtctactca cctcttcctc gagcataact tccaagctct cttcagccag atctttatgc
     5941 ccaacgtcgg aagcaggacc gcggctgaac gagctgggta cgtgcagtgg ggcatcaggc
     6001 cctatgctgg ctaccataac ggtacgtatc ttgacctgtg ggagggagtt ctcacacgcg
     6061 agcggtggca acaactcagt cagtagcgac gaaaggaaag aacatgttcg aactagccgt
     6121 ggatgaagac atccgtctca tccttcgaga ccccaatgag gcggaagctc ttttctccct
     6181 tatcgaccgt aaccgcggtc acttgcgtcc ctggttcaat tgggtcgaca cgacgcagag
     6241 cgttgatacg acccggaaat accttgagat gtgtgtccag ggtttcgaag ctggaactct
     6301 tgtagatctc gggattcgtt accgggacga gtgggttgga tcgctcagcc tccacgccat
     6361 tgagaaagcg agcagaaaag ccgagattgg ctattgggtc gcccaagacc accaaggcaa
     6421 aggaattgta acccgagcgg tgcgggcact cattgagtat ggattcggta gcctcaacct
     6481 caaccgggta gcaattctct gcaacgtt
//
LOCUS       DASWYB010000054         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_750760, whole genome shotgun sequence.
ACCESSION   DASWYB010000054 DASWYB010000000
VERSION     DASWYB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750760"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGE59_03155"
     CDS             complement(<1..506)
                     /locus_tag="VGE59_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY1074669.1"
                     /translation="MKQWHDGPQVTHSLVDHIVKARSLSLDDRFLSPRWPEDLHDTFL
                     LPDMDKATARIWKAIEAGETIGIVGDYDMDGTPAAVIMAQFFRLVGSDVHVILPTREE
                     GYGFSTTFVDRLREKKVTLIITVDCGIRDSAAVEHANQHGCDVIITDHHECAELLPLA
                     LAIVDPKRV"
     gene            complement(503..1273)
                     /locus_tag="VGE59_03160"
     CDS             complement(503..1273)
                     /locus_tag="VGE59_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074670.1"
                     /translation="MAHRTQVHRMPKIIRGGRIESERPKPSRKSFGLLFIGLAVVVGY
                     WFLFMSDTFAVQKVEVAGAESPSITSVAEALKGRNIFRLTSVSIEQDIRQSYPPTDSV
                     LVVRGLPNTVRLTVSLREPALQWQAGEALFILDSQGEAFEQGTTKPEYAALPKIIDRS
                     NRPVSIGQQIVTPAYVTFVQDINKRAPDLFKRAHVANEILETTFHIDIVLEGDLRIRV
                     TTQRPLEEQLLGAASIYTAHPDAKTIDVRVPKRGYYKK"
     gene            complement(1334..2314)
                     /locus_tag="VGE59_03165"
     CDS             complement(1334..2314)
                     /locus_tag="VGE59_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY1074671.1"
                     /translation="MKLRDHVPLREVDWFHTGGVAEYYVECQTLAEVIEATKQAAELG
                     VEYRVIGEASNLLMSDGGYPGLIIQNRASSIVFLHDRSQIIVDSGTLISTLVSQTISQ
                     GYSGLEFLAGVPGTIGGAVYGNATAFGRSISEHVRAATLLAPSSKTDADPVRRVDRDW
                     FAFGYRTSKLKQLRESHSTTNGPVILSVTLQLSKMSYSSCLQRMHYYQKMRASYPIAQ
                     PKMLYIFDSLCLTGEEVSQRTMGGRRDVPCHLIERNNLRQCKVNEIISYPKNPNFIIN
                     TGVGTSRDAAVLIEYIEQALGPTVHPLVSHIERVGLWDTPASSSVQGDGV"
     gene            complement(2320..3720)
                     /locus_tag="VGE59_03170"
     CDS             complement(2320..3720)
                     /locus_tag="VGE59_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY1074672.1"
                     /translation="MIPYRFIHFVGALSPDVAALAVYTAQSGIKVTASDDHNDNLYAP
                     ALQAAGVEFFDMFAQTNIQKTTELVVLSPYYDDKHIEAVVALNAKIRVITAIDYAKLL
                     SEQAQRLAIVSDYEGPIIATLLAHVWNQAYMPIRGLTKTITVENPSLSVVNEADWFVH
                     PLSGFKRDATTYEADFLSLEAQTVIIPSIMYDYPELNTTLDEVYQSYYTFVKRIPRKG
                     LIIGNSDWSRMKRLRTHLADRHIETYGFDRDAMWQIRDVTTENGSTSFSLFSNRHLYG
                     PFTIPYCGTLYTYAAAAVCVASLTVDMKPEVLGRGLLSAPKLKRYFEQHTDKEGRALI
                     VDRADHPELIHSVLETVKALYPDKRIWCVYQPGSFLRTKALSDDLEQALKLADSVYLA
                     DITGYPREKSEGLHIRHFIAHMKATHPQTYYSDESVDIAQLLSDRVPSSDCIVVLGAE
                     TLIDHVLNHLLPQGEE"
     gene            complement(3732..4829)
                     /locus_tag="VGE59_03175"
     CDS             complement(3732..4829)
                     /locus_tag="VGE59_03175"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEY1074673.1"
                     /translation="MSSRTKKIWLVGGGTGGHVVPLLAVAEVLKKDTSISLTFIGEKG
                     GQEATLARQYGLRFIGIRSGKLRRYITLGAFFLNLRDIIQFLRGLRASYILIKKEKPS
                     LIFTKGGYVTLPVALAAYLTSTPLVIHESDAVMGMTNRWLARLATVVMTGFSAVHYPA
                     FLAPKIQAVGIPLRSEFSRKRSVTRSSRPMILITAGSQGAVAINMAMAEIIPLLLKRA
                     SVMHITGRYSYERFRAFKESLPPHISEHYGVVDFTPDMADYMREATVLIMRSGSQIFE
                     GASLGKPMVLIPLPGSANNHQARNAEIFAQHKAALVLNQHNLTPERLYETINSVLDDA
                     ALRHELEEGTRVFNSCDSAKRVAEILKQQLR"
     gene            complement(4831..>5167)
                     /locus_tag="VGE59_03180"
     CDS             complement(4831..>5167)
                     /locus_tag="VGE59_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY1074674.1"
                     /translation="LPEPHTDSIFAIIVEELGFVRSLLVLLVIGALAYRGYQIASRAG
                     DDFSRMVAFGITSWLLIQAVINVAAIMGLVPLTGVPLPFISYGGTSLIASLVGVGIMV
                     NISKQSYGK"
BASE COUNT         1408 a         1246 c         1163 g         1350 t
ORIGIN      
        1 actctcttgg ggtctacgat tgcaagggct agtgggagca attcagcgca ttcgtgatga
       61 tcggtaataa taacatcgca cccatgttga ttggcgtgct cgactgcggc agaatcccga
      121 ataccacaat cgacagtgat aatgagtgtc acctttttct ctcgtagccg atcaacaaat
      181 gtggtcgaaa aaccgtatcc ttcttctcga gtcggtaaaa tgacatgaac gtcgctgcca
      241 actaaacgga aaaactgtgc catgattacc gccgctggag tgccatccat atcataatcg
      301 cctacaatac caatcgtttc acccgcttct attgctttcc aaattcgagc tgtggcctta
      361 tccatgtctg gtaacaaaaa agtatcatga agatcttcag gccatcgagg agacagaaaa
      421 cgatcgtcga gtgacaaact tctcgccttt acaatgtgat cgactaatga atgagtaact
      481 tgagggccgt cgtgccattg tttcatttct tataatatcc tcgttttggt acgcgcacat
      541 caattgtttt cgcatccgga tgcgctgtat aaatagaagc tgcccctaga agttgttctt
      601 ctaatggccg ctgcgttgta acgcggatgc gcaaatctcc ctcaagcaca atatcaatgt
      661 ggaatgtggt ttcaaggatc tcattggcca catgagcccg tttaaaaaga tctggtgctc
      721 ttttgttaat atcttgaaca aaggtcacgt aggcgggagt aactatctgt tgcccgattg
      781 aaactggacg attggatcgg tcaataattt taggaagggc ggcatattca ggtttcgtag
      841 taccttgttc gaaagcttcc ccttgactat cgaggatgaa caaggcttct cctgcttgcc
      901 attgaagtgc tggctctcgc agactgactg ttaaacgtac cgtatttggc aaccctcgca
      961 ccaccagtac gctatcggtc ggtggatagc tttggcgaat atcctgttct atcgagacgc
     1021 ttgtcaaacg aaagatgttc ctccccttca acgcctccgc aacactcgtg atcgaaggcg
     1081 attcggcccc ggctacttct actttttgaa ctgcaaaggt atcgctcata aagaggaacc
     1141 agtacccaac tactacagcc aaaccaataa aaagtaatcc aaagctcttg cgagatggct
     1201 taggacgctc actttcaatc cttccccctc gtatgatctt tggcatgcga tgaacttgag
     1261 ttcgatgtgc cattttccct cttatttgta acacctaaat acaccacgca atgaagagtg
     1321 taatcctata atatcacact ccgtctccct gaacggacga ggaagcaggc gtatcccata
     1381 gtccgacccg ttcgatatga gagacgagtg gatgaaccgt cgggcctagt gcttgttcaa
     1441 tgtattcaat aagaacagca gcgtctcgac tcgttccgac gcccgtattg attataaagt
     1501 taggattttt tgggtaactt ataatttcgt taaccttgca ctgtcgaagg ttatttcttt
     1561 caattaaatg acacggcaca tcacgccttc cgcccatagt acgctggctc acttcttccc
     1621 ccgtaagaca gagggaatca aaaatataga gcatcttcgg ctgcgcgatg ggataagagg
     1681 ctctcatttt ttggtagtag tgcatgcgtt ggagacaaga gctatagctc attttcgaga
     1741 gctgaagcgt tactgacaaa ataacaggtc cgtttgtagt agagtgagat tcccggagct
     1801 gtttaagctt actcgtacga tagccaaagg caaaccagtc tcgatccacg cgacggacag
     1861 gatcggcatc ggtttttgag cttggggcaa gtaatgttgc ggcacggaca tgctcactaa
     1921 tggaacggcc aaaggctgtt gcattcccat acacagcgcc acctatcgtt ccggggacgc
     1981 ccgctaaaaa ctctagtcca ctgtaccctt gggaaatcgt ctgcgacaca agtgtagaaa
     2041 tgagggttcc ggaatcgaca atgatttgtg accgatcatg gagaaacaca atcgatgagg
     2101 ctctattttg aataatgagc ccaggatacc cgccgtcact cattaaaagg ttcgacgctt
     2161 caccaatcac ccgatactca acccctaact ctgcggcttg tttcgtagct tctataactt
     2221 cagcgagcgt ctgacactca acataatact ctgccactcc gcccgtatga aaccaatcaa
     2281 cctctcgcaa aggaacatga tcgcggagct tcatcgagat tactcttctc cctggggaag
     2341 caaatggttt agaacgtgat caatgagagt ttcggcaccc agtaccacaa tacaatctga
     2401 cgacgggact ctgtccgaga gaagctgcgc gatatctacc gactcatcac tatagtaggt
     2461 ctggggatgc gtcgctttca tatgggcaat aaaatgtcga atatgaagac cttctgactt
     2521 ttctcgagga taccctgtta tatcagccaa atacacacta tcggcgagtt taagagcttg
     2581 ttctaagtcg tcagacaatg ctttcgtgcg taaaaacgag ccaggttgat atacgcacca
     2641 gatccgcttg tctgggtaca gcgcttttac ggtttcgagg accgagtgaa tcagctccgg
     2701 atgatcggcg cgatctacaa tcaaagcacg tccttcttta tcagtatgct gctcaaagta
     2761 acgtttcaat tttggagcac tcagcaaccc tcggcctaat acttcaggct tcatgtccac
     2821 tgtaagagag gctacacaca ctgcggcggc ggcgtatgta tagagagtgc cacaatatgg
     2881 gatggtaaat ggtccgtaaa ggtgccggtt agaaaataat gaaaagctcg ttgaaccatt
     2941 ttcggttgtt acatctcgga tctgccacat ggcgtctcgg tcaaaaccgt acgtttctat
     3001 atgtcgatct gccaaatgag ttcgtagccg tttcattcgg ctccaatcag aatttccaat
     3061 aataagccct tttcgaggaa tacgttttac aaaagtatag taggattgat atacctcatc
     3121 aagggttgta ttaagctccg gatagtcata cataatactg ggaatgatga ccgtctgtgc
     3181 ttcaagagaa agaaagtcgg cttcgtatgt tgttgcatct cgcttaaatc ctgacaaagg
     3241 atgaacaaac cagtcagctt cgttaacgac cgacaaggaa ggattctcga ctgtgatcgt
     3301 tttggttaat cctcgaattg gcatatatgc ttggttccac acatgagcga gcaatgtagc
     3361 aataatagga ccttcatagt cgctcacaat tgctaaacgc tgcgcttgct cactgaggag
     3421 tttggcgtaa tcaatagctg taataactcg tattttcgca ttaagagcca caaccgcttc
     3481 tatatgcttg tcatcataat atggggagag aacgaccagt tctgtcgttt tttgaatatt
     3541 tgtttgggca aacatatcaa aaaattcaac tcctgctgcc tgtagggctg gagcataaag
     3601 attgtcgtta tggtcatcag aagcggtaac ctttatacct gactgagcgg tatatacagc
     3661 aagggcagca acgtcgggtg agagtgcccc aacaaaatga ataaaacggt agggaatcat
     3721 agagcgaact cctagcgtaa ctgctgtttt aaaatttcag caacgcgctt cgctgaatcg
     3781 cagctgttaa aaactcgtgt tccctcctcg agttcgtgac gcagcgcggc gtcatctaaa
     3841 accgagttga tggtctcata caagcgttca ggcgtcaaat tatgttgatt caaaacaagg
     3901 gctgctttat gctgtgcaaa tatttccgcg ttgcgggctt ggtggttatt tgccgatccc
     3961 gggagaggta ttaaaaccat cggtttccct aaagaggcgc cctcaaaaat ctgagagccg
     4021 gatcgcataa tgagtacggt tgcttcacgc atgtagtcag ccatatcggg tgtaaagtcc
     4081 accacgccat aatgctccga aatatgagga ggcaagctct ccttaaatgc tcgaaaacgc
     4141 tcgtatgaat atcttcctgt aatatgcata accgaggcac gtttgagtaa caaaggaata
     4201 atttctgcca ttgccatatt tatcgccact gccccctggc tccccgcggt gataagtatc
     4261 ataggccgag agctccgagt cacactacgt ttgcgtgaaa attcggaacg taatgggatc
     4321 cctactgcct gaatttttgg agccagaaaa gcggggtagt gaaccgccga gaatcctgtc
     4381 ataacaactg ttgcgagccg tgctaaccac cggtttgtca tccccatcac ggcatccgac
     4441 tcatgtataa ctagcggggt ggacgtcaag tacgctgcta gagcaacggg aagagttaca
     4501 tacccccctt ttgtaaagat aagcgatggc ttctcttttt tgattaagat atagctggct
     4561 cgtaatcctc gcaaaaattg aatgatgtct cgaaggttaa gaaagaacgc tccaagcgtt
     4621 atgtaacgtc gtaactttcc actacgaatg ccgatgaacc gaagcccgta ttggcgtgct
     4681 aacgtcgctt cctgtccccc tttttctcca ataaaagtca atgaaatact tgtatctttt
     4741 tttaagactt cggcaactgc caaaagagga acaacgtgtc ctccggtgcc ccctcccaca
     4801 agccagatct tttttgtacg gctactcatt ttactttccg tagctttgtt tcgagatgtt
     4861 aaccataata ccgactccca caagtgaggc aatcaacgaa gtccccccat agcttataaa
     4921 gggtaaaggc acacccgtaa gaggtactaa ccccataatc gccgcaacgt taataacagc
     4981 ttggattaaa agccagctcg taataccaaa ggcaaccatc cgggagaaat catcacctgc
     5041 ccggctcgca atctggtaac ctcgataggc taaagcgcca atgacgagaa gaacgagcag
     5101 cgatcggaca aagccaagct cttcgacaat aatggcaaaa atcgagtcgg tgtgcggctc
     5161 tggcaaa
//
LOCUS       DASWYB010000055         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_286317, whole genome shotgun sequence.
ACCESSION   DASWYB010000055 DASWYB010000000
VERSION     DASWYB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286317"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VGE59_03185"
     CDS             complement(<1..557)
                     /locus_tag="VGE59_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074675.1"
                     /translation="MINKRIIAIAAGIAGFLFLGALFYFVFQTFFSGSGTITVWTIEG
                     NEQAIKEVAALYRQKHKGYKVKIVPVSEQVYEFTSLYALATQKGTADRPAPDVWIMPN
                     EWLTQHRDKLESAPDGTLDKAVQASFTKGSSSNLPKGRSNTEIITRDYAKIVEQDVIA
                     DNKVYGIPLNMDTLALYYDRTKINPA"
     gene            complement(592..1161)
                     /locus_tag="VGE59_03190"
     CDS             complement(592..1161)
                     /locus_tag="VGE59_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074676.1"
                     /translation="MISWKDLSLPPKIIALIIAIILGGGTLFFTFSFVYGRVTLSSSS
                     ANAFTFTESERTPQALPLTLRKKPGKYLYVVGAPGKLNTPVVVKVYAFFHRKLNVALP
                     VDYSTEQDHFRIMYDYENESYQIVPYINFGPDLSPREQLKTQWTTYEVYAKEAIAYLK
                     AQGADTKKLPVMWWAKEWWPEGKALDSLL"
     gene            complement(1171..2355)
                     /locus_tag="VGE59_03195"
     CDS             complement(1171..2355)
                     /locus_tag="VGE59_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY1074677.1"
                     /translation="MNPQVTNPSQEPSQWPPAWFNFFLVLVLLFALLIGLAMSAFFVG
                     ERQRAVGENVAGSDGRVAAGSGTGSTSDCSGVASVPTDLRSHVRDAAQKHLGGDEAAL
                     IALIQIESSWVVTAKSSGSSAAGLGQFTNPTARDYKEFVGGSDNVGITWAGGVVYDSP
                     SEHSDDARFDAKRSIYATAHKLKVELTRFGTLEQAYIEGYHTHTTAAQLAEAQAGAAR
                     LMDAYKALKNGGGCTSTETNTSGGSSSTSSSLGTQIVADARKYIGQTTQVQADGENIF
                     QCDAPTRSCASFVSTVLLDAKAITFHEKGAQALWDRTVSVQQVLPPGSRVDLSQLQPG
                     DIVWFGSGRFQHVGVYSGNGRVIHTSSIAGKPGSYARVAEANLSGVADSMGGAIAAKR
                     FK"
     gene            complement(2369..>3084)
                     /locus_tag="VGE59_03200"
     CDS             complement(2369..>3084)
                     /locus_tag="VGE59_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074678.1"
                     /translation="FSGLFTQQTNFDLNRGMVVFSVRDLDDELRPVGMYLVLNYIWNR
                     VRFNPHKRILIVDEAWFMMQHEDSAKFVYSIAKRCRKYFLGLTVITQDVEDFLNSQYG
                     RSVITNSSMQVLLKQSPAAVDLLADVFNLTDGEKLWLLEAGVGEGLFFAGTNHVAIKV
                     VASYNEDRLITTDPHQLLEIQEGEAMMEANSQAAEAAFAESEAPIPGVPTEPTATEDA
                     AVETSPSVNQTPPTETPSA"
BASE COUNT          804 a          777 c          721 g          782 t
ORIGIN      
        1 gcaggattaa ttttcgttcg atcatagtaa agggccaacg tatccatatt aagaggaata
       61 ccatacactt tgttatcggc aatgacatct tgctctacga tcttggcata gtcgcgggta
      121 ataatttctg tgttacttcg gcctttcggc aagttggacg atgaaccttt cgtaaaactt
      181 gcttgcaccg ccttgtcgag tgtcccgtcg ggggcacttt ctagtttatc gcgatgttgc
      241 gtgagccatt cattaggcat aatccatacg tcgggagcgg gccggtcggc cgtccctttc
      301 tgggttgcga gcgcatacaa agaggtaaat tcgtatactt gttcggaaac aggcacgatt
      361 ttgactttgt agcccttatg cttctgacgg tagagggctg caacttcttt aatcgcttgt
      421 tcattccctt caatcgtcca gactgtgatc gtaccgcttc cactaaaaaa ggtttggaag
      481 acaaagtaga aaagggcgcc taaaaaaagg aatcctgcga taccagcggc gattgcaatg
      541 atacgcttat ttatcaaaat ttccctccat cacacttctc atcataacac actatagtag
      601 gctatcgagt gcctttcctt ctggccacca ctccttcgcc caccacataa ccggtagttt
      661 cttcgtatca gctccttgtg ccttcaagta ggctatcgct tctttcgcgt acacctcata
      721 ggttgtccac tgcgtcttga gttgttcgcg gggagataag tctggtccaa agttaatata
      781 tggcacgatc tgataacttt cattttcgta gtcgtacatg atacgaaaat gatcctgctc
      841 tgtcgagtaa tcaaccggca atgctacatt caacttgcga tggaagaaag catacacctt
      901 cacgacgaca ggtgtattga gtttcccggg cgcccctacc acataaaggt attttcctgg
      961 cttcttcctt aacgtgagag gaagagcttg aggcgttctt tctgattctg taaaagtaaa
     1021 tgcattggcc gaagatgagg acaaggtaac tcggccatac acaaacgaga aggtaaaaaa
     1081 gagcgttcct ccacccaaaa taatagcgat aattaaagca ataattttag gtggaaggga
     1141 gagatctttc caagaaatca tagtagctcc ttacttaaac cgttttgcgg caatagcacc
     1201 gcccatagaa tcggctacgc cgctcaaatt cgcttcggca actcgcgcat aactgcccgg
     1261 tttcccggca atagaactcg tatgaatcac cctaccgttg ccggagtaaa cgccaacatg
     1321 ctgaaaacga cctgacccaa accaaacaat atctcctggc tgcaactggc ttaagtccac
     1381 ccggcttccc ggcgggagaa cttgctgaac agatactgtt ctatcccaaa gggcttgagc
     1441 ccctttttca tggaatgtga tagccttggc atcgagaagg acggttgata caaacgaagc
     1501 acacgaccgg gtgggcgcat cgcactgaaa gatgttttct ccgtcggctt gaacttgagt
     1561 tgtttgccca atatactttc gagcgtccgc cacaatttgt gtcccaagcg aggagctggt
     1621 ggatgagcta cctcctgatg tatttgtttc ggttgaagta cacccaccgc cattctttaa
     1681 tgccttatag gcatccataa gccgcgccgc gccagcttgt gcttctgcta attgagcagc
     1741 ggtagtatgg gtatgatagc cttctatgta ggcttgctcg agcgtaccaa aacgagttaa
     1801 ctccacttta agcttatggg ccgtcgcgta aatcgatcgc ttcgcatcaa aacgggcatc
     1861 atccgaatgt tctgaagggc tgtcgtacac taccccgcca gcccacgtta taccaacgtt
     1921 gtcacttccc ccgacaaatt ctttatagtc gcgcgcggtg ggattcgtaa attggcctaa
     1981 gcccgcggct gaagaacctg agctcttcgc cgtcaccacc cagcttgatt cgatctggat
     2041 aagtgcaatg agcgcggctt catcaccccc aaggtgtttt tgggcggcat ctcggacatg
     2101 cgatcgtaaa tccgtgggaa cagaagcaac gcccgagcag tcagacgtcg atcccgtacc
     2161 cgatccggca gctacccgtc catcagaccc ggcaacattt tccccaacgg cccgctggcg
     2221 ttctcctaca aaaaaagcac tcattgccaa cccgattaac agcgcaaaga ggagaactaa
     2281 tacgagaaaa aagttaaacc atgcgggagg ccactggctt ggctcttgag atgggttagt
     2341 aacttgagga ttcatactga actatcaatt atgcggaggg agtttcagtc gggggagttt
     2401 gatttaccga aggagacgtc tcaacagctg catcttcagt cgcggttggt tctgtaggca
     2461 caccaggaat aggcgcttcc gattcagcaa aggcggcttc ggcggcttgc gagtttgctt
     2521 ccatcatggc ttcaccttct tgaatctcta agagctgatg cgggtcggtc gtgattaaac
     2581 gatcttcatt atacgaggcg actactttaa tggcgacatg attggtaccg gcaaagaaca
     2641 acccttctcc gacacccgct tcaaggagcc agagtttttc tccatcagtt aagttaaaaa
     2701 catccgcaag taagtcgacg gcggcaggag actgcttcag aagcacctgc atcgaagagt
     2761 tggtaatgac cgaacggcca tactgcgaat ttaagaaatc ttccacatct tgagtaataa
     2821 ccgtaaggcc taagaaatat ttgcggcaac gtttagcgat ggagtaaaca aatttggcag
     2881 agtcttcgtg ctgcatcata aaccaagcct cgtcaacaat aagaatacgt ttgtgcggat
     2941 tgaatcgcac acggttccaa atgtagttca agactagata cataccgact ggacgaagtt
     3001 catcgtctaa gtcacgcacc gagaagacga ccattccgcg gttcaaatcg aaatttgtct
     3061 gctgggtgaa gagacccgag aaac
//
LOCUS       DASWYB010000056         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_345731, whole genome shotgun sequence.
ACCESSION   DASWYB010000056 DASWYB010000000
VERSION     DASWYB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345731"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VGE59_03205"
     CDS             <1..1330
                     /locus_tag="VGE59_03205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1074679.1"
                     /translation="SGSGGSVGAHHAASLGKDVAIVERDTIGGECPNYGCVPTKALLY
                     AAKTYESAESAAFYGTRMGGKVSYDYNAVRKWKNFVVSRTGAAQGEKVFEDSDIHVLN
                     GEAKFVSPHEIRIGTRRYSADKFLIATGTDVAIPPIPGLDAAGYVTFREAINFKQLPD
                     SLFILGGGAIGCEFAQVFSSFGVKIHIADFAERLLAKEDEEVSAIVQAIFENRGIKVH
                     TAARVLRISKREGKKVVHYEVAGKEHAAEVDEILVATGKRPMLDLDLERAGVAHDKHG
                     LKVNAYLQTSASHIYAAGDVIGPLMFTHTASYQSYIAANNMFSRSKIKPSYQVIPRCV
                     FLEPEVASVGISEAEAIERGFKIKKGMAAIASLGRANTDNTLDGFVKVITKTDGTLLG
                     ASIVSPRAGEMIHELALAMQLKAKASDVAAMVHAYPTYSEAVKYACANIE"
     gene            complement(1335..1598)
                     /locus_tag="VGE59_03210"
     CDS             complement(1335..1598)
                     /locus_tag="VGE59_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074680.1"
                     /translation="MKKEDTLSAFDNQLRDIVIALIEIIDSTDNPKTLIQLDKLVSEL
                     RPLLDLSNLNSTLGEELWLYVGIKKDANAEMANDYRESLRSLI"
     gene            complement(1601..2812)
                     /locus_tag="VGE59_03215"
     CDS             complement(1601..2812)
                     /locus_tag="VGE59_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1074681.1"
                     /translation="MIENIKTALSAVWSNKVRSILNVLGVVIGVSSVTILISLGEGLK
                     NDVSSLIGDFGTNLVFVTGGKIDTSKGAANNTNPANFISGDVLSIKDTETIDALPEIK
                     AVSPSSIVSGTLKYEDKTSSSTIMGTYPNMLDALNILAIDKGQLFTSKNAGDVIVLGK
                     SATDALFGDTNPIGKKVLLGQREMEVIGTIKAKKSNSVFGSEFDAMSLVPFDTATTIN
                     KGQVKVFRIFATTNKPEDIEPAKKDIKAGLIANHNGEEDFSVLTQDDMLGLFNSFLNL
                     ATTMVSAIAAISLLVGGIGIMNIMLVTVTERTKEIGLRKAVGATKVAILTQFLVEAIV
                     VTFLGALIGLAIAFVADMVIINQTELTPTITPGIILLSVAISIGIGIIFGLWPAARAA
                     NKDPIEALRYE"
     gene            complement(2805..3488)
                     /locus_tag="VGE59_03220"
     CDS             complement(2805..3488)
                     /locus_tag="VGE59_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074682.1"
                     /translation="MTLLSLQHVDKIYQSGKILLPVLHNIDLEIKKGEMVAIMGPSGS
                     GKSTLMNIIGLLDRPSNGTLMIDGETVDLSMPDRKLAMLRNKKIGFVFQSFNLLPRIS
                     ALGNVLVPTAYAKQNRKQATARALNLLEKVGLKDRSHHKPTELSGGERQRVAIARALI
                     NNPEIILADEPTGNLDSRSGAEIMTILRELHKEGKTVIIITHDAEIGKQCQRTVRIQD
                     GEIAGGKHD"
BASE COUNT          938 a          753 c          874 g         1077 t
ORIGIN      
        1 ttctggatcg ggtggaagcg ttggtgccca tcatgctgct tcgcttggaa aagatgtggc
       61 aattgtagaa cgagatacga tcggcggaga atgtccgaac tatggctgcg tccctaccaa
      121 agcactcctt tatgctgcta aaacctatga atcagccgaa tcggcggcgt tttatggaac
      181 gagaatggga ggcaaagttt cctacgatta taatgccgtt cgaaaatgga agaattttgt
      241 agtttctcga acaggtgctg cccaaggaga gaaagttttc gaagacagcg atattcatgt
      301 tttaaacggt gaggcgaagt ttgtatctcc gcatgagatt cgtatcggta ctcgtcgata
      361 ttcggcagat aaatttttaa ttgcgactgg gacagatgtc gcaattccac ctatccctgg
      421 tttagacgcg gccggatatg ttaccttccg agaagccatt aattttaaac agctccccga
      481 ctcgttgttt atccttggtg gtggtgctat tggctgtgaa tttgctcagg tcttctcgag
      541 ctttggcgtt aaaatccata ttgccgactt tgctgaacgc ttgctggcga aagaagacga
      601 agaagtaagt gcgattgttc aggcaatttt tgaaaaccgc ggtattaagg tacatacagc
      661 cgctcgtgtt ctccgtatct ctaaacgtga gggcaagaag gttgttcact atgaggtagc
      721 gggcaaggag cacgctgcag aagttgatga gatcctggtg gcaacgggta agcgccctat
      781 gctcgacctt gatctcgaac gagctggcgt tgcccatgat aagcatggtt taaaggtcaa
      841 tgcttactta caaacgagtg catcgcatat ttacgccgcc ggtgacgtta ttggacccct
      901 catgtttacc cataccgcga gttaccaaag ctatattgca gccaataata tgttttcgcg
      961 aagtaagata aagccaagtt atcaggttat tcctcggtgt gtttttcttg aaccagaagt
     1021 tgcttcagtt ggtatttccg aagccgaagc gatcgaaaga ggtttcaaaa ttaaaaaagg
     1081 aatggccgca atcgcttctt tgggacgggc taataccgac aataccctgg atggatttgt
     1141 caaagtgatt accaaaacgg atggcacgct tctcggggcg tcgattgtct ctccccgagc
     1201 tggtgaaatg attcatgagc ttgctctggc gatgcagctt aaggcaaaag cctctgatgt
     1261 tgccgcaatg gtccatgcat atcctactta ttcggaagcg gttaagtatg catgcgcaaa
     1321 tatagaatag caaattatat gagtgatcgg agactctctc ggtagtcgtt ggccatctct
     1381 gcattggcat ctttctttat tccaacatat aaccataact cttctcctag agtagagttg
     1441 aggtttgaaa gatccagaag tggtcggagc tcgctcacga gtttatcaag ttgaatgagt
     1501 gttttggggt tatcggtact gtcaattatt tcaataagag cgattacaat atctcgtaat
     1561 tggttatcga aagcagatag agtgtcctct ttcttcatga ttactcgtaa cgtaatgctt
     1621 caatcgggtc tttgtttgcg gcgcgggcag caggccagag gccaaagata atgccaatac
     1681 cgatagagat agcaaccgac aaaagtatga ttcctggggt aatggtaggg gttagttctg
     1741 tttggtttat aattaccata tctgcaacaa aggcaatagc taagccgatc aaggccccaa
     1801 ggaatgttac tacaatcgct tccacaagga actgagttaa tatggcgacc tttgttgcgc
     1861 cgactgcttt gcgtaggcca atttcttttg ttctctcggt aaccgtcacg agcataatgt
     1921 tcataatgcc gataccgcct acaagcaaag aaatagcggc aatggcggaa accatggtgg
     1981 ttgccaagtt aaggaagcta ttaaagagac caagcatgtc gtcttgcgtg agaaccgaaa
     2041 aatcttcttc tccattgtgg ttggcgatga ggccagcctt aatatctttt tttgcgggtt
     2101 caatatcttc tggtttgttc gttgtcgcga agatacggaa cactttaact tgacctttgt
     2161 taattgttgt cgccgtatcg aacggtacga gtgacatagc gtcaaattca ctcccaaaaa
     2221 cgctgttcga ttttttggct ttgatagtgc caattacttc catttcccgt tgtcctaata
     2281 ggactttttt accaatgggg tttgtgtcac caaaaagagc atcagtggca cttttcccga
     2341 gtacaatgac gtcgcccgca tttttcgagg taaaaagttg tcctttatcg atagcgagaa
     2401 tgttaagggc gtcgagcata ttggggtatg ttcccataat agttgaagaa ctcgtcttgt
     2461 cttcgtattt taaggttccc gacacaatac tacttggtga gacggcttta atttctggca
     2521 atgcatcaat tgtttcagtg tctttaatgc tcagcacgtc accgctaatg aaattggcgg
     2581 gatttgtgtt atttgccgca cctttgctcg tatcgatctt tcctccggtc acaaagacta
     2641 agttggtgcc aaagtcccca attaaacttg aaacatcatt cttgagtccc tcgcctaagg
     2701 agataaggat ggtgacggat gagacgccaa taacaactcc gagcacattt aagatactac
     2761 ggactttgtt gctccacact gcactgaggg cggtctttat attctcaatc atgtttccct
     2821 cctgcgattt ccccatcttg tattcgtact gttctttgac actgtttccc tatttctgcg
     2881 tcgtgagtaa tgatgataac ggttttcccc tctttgtgga gttctcgtaa tatcgtcatg
     2941 atttcagcgc cacttcgtga gtcaaggttc cctgttggtt catcggcgag aataatttcg
     3001 gggttgttaa taagggcgcg agcaatggca actcgctgtc tctcgccccc ggaaagttcc
     3061 gttggcttgt gatgtgaccg atctttaagg ccaactttct caagaaggtt tagagcccgt
     3121 gcggtagctt gtttacgatt ttgttttgcg taagcggtag gtacgagcac attgccaaga
     3181 gcagatatgc gtggaagtaa attgaaagat tgaaagacaa acccgatttt tttatttcga
     3241 agcatagcga gttttcggtc tggcatcgat aggtcgacgg tttctccatc gatcatgagg
     3301 gttccattag agggtcggtc taggagacca ataatattca taagcgtgga tttgcccgaa
     3361 ccagagggtc ccataatggc gaccatttcc ccctttttta tttcgaggtc aatgttatgg
     3421 agaacaggca aaagaatttt acctgactga taaatcttat cgacatgctg aagagaaagt
     3481 aatgtcattg gtatcctttg tgttgagtaa gtttattata gtcctgcgag tgaaaaagta
     3541 aaattaagtc tgaaagggaa tcaattttat cagctgccaa caatttgact caaggcgata
     3601 tagctttatt gcgttgacaa taaaacgtgt tggtgtgaag tc
//
LOCUS       DASWYB010000057         5625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_449879, whole genome shotgun sequence.
ACCESSION   DASWYB010000057 DASWYB010000000
VERSION     DASWYB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449879"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2313
                     /locus_tag="VGE59_03225"
     CDS             <1..2313
                     /locus_tag="VGE59_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY1074683.1"
                     /translation="LGLSGAVMVYGRDLPSDQTLTQWKPAEATRIYDRNGTLLYELYG
                     NEKRVVVSGDDISPYLRFATVAIEDQRFYDHYGIDPRGILRAAVRNAQHDNRTEGGST
                     ITQQLAKNVYLSPRRTFDRKIREAMLAIKLEQHYTKDQILTLYLNQVGYGSNAYGAEA
                     ASQMYFGKPAKDLTLAEAATLAALPKSPGTLSPYSSTTDRLIARRNTVIKKMVEMHYL
                     PPQEAVLATQQKLAVKPREEKITAPHFVMYVKEKLIEKYGEEMVLNGGLQVTTTLDLS
                     KQQLAEKIANESTAHLSRAGATNMGLVAVEPKTGEILAMLGSKDYFDVAHDGNVNVTI
                     SSRPPGSSFKPVVYAGLMEKNGWGPGSTLFDVETDFGDASFPYVPNNYDEKYHGPVSI
                     REALANSYNVPAVKAMSLIGKEDTISLAQRLGITTLTDPNRYGLSLVLGGGDIRLLEL
                     AGAYTSFANEGAYSQPVAIRKVVQGDHVLFEHYSEPKPVFRPEVAYEVSSILSDPVAR
                     SRVFGTRTPLNIAGHTVAVKTGTTQEYRDAWTVGYTPSLVVGVWVGNNDYHPMKSGSA
                     GAMAAAPLWNTFVSQALAGTPNEDFVAPSGIQIATIDAITGKLPTDITRETRQDLFAS
                     WQLPGQVELASFQIYDCNGQVKATKRSYVVTSERPNDPNWEKPVLEWARTHGYPVRHQ
                     GDIHEPCPTPSVEVVEVQNPEEAALGGIPAELSPTFVIESTPKPTPEVQVTPTPRPSA
                     EEPTPSPSPTVEPTDNQSRTPSTRTSRRRD"
     gene            2396..3571
                     /locus_tag="VGE59_03230"
     CDS             2396..3571
                     /locus_tag="VGE59_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY1074684.1"
                     /translation="MAKTEKPLFQSFFWGGFECASHIGLTRTRWDLLGMSRHDILCRE
                     DYQRLKELGITTVREGLAWSQIDLGKNKYDFSRYESMMKVAHEEGIEQVWDLNHFDYP
                     EYLDPFSERFVIQFARYAQKAIKLIRKYNPNRTLYIVPINEISFWSHMGGMIGVWAPH
                     KLTRGYQFKQQLVRASIAAMDAIWEIDSNVRFIQVDPIFMRTAKKPISIVKKATEDSF
                     REIKFQAFDMLCGKLKPELGGHPRYLDIIGANYYLYNQEWITGDDPLDDSCHELIPWN
                     SRARVSLATMLLEVYSRYHRPIVVTETGAWGDWREKWWKRTLKEIDDALIEGLPLYGL
                     CAYPVIDRHDWDDAHLTNSGLWDFEEGDSRCRRIPHEASLQAIRPYIEKWHKLTSTG"
     gene            complement(3632..3705)
                     /locus_tag="VGE59_03235"
     tRNA            complement(3632..3705)
                     /locus_tag="VGE59_03235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3671..3673),aa:His,seq:gtg)
     gene            complement(3716..3791)
                     /locus_tag="VGE59_03240"
     tRNA            complement(3716..3791)
                     /locus_tag="VGE59_03240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3756..3758),aa:Arg,seq:tct)
     gene            complement(3866..4276)
                     /locus_tag="VGE59_03245"
     CDS             complement(3866..4276)
                     /locus_tag="VGE59_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1074685.1"
                     /translation="MENRPKVGVGVFIRKENKVLLIKRRGSHGEGTWGLVGGHLEFGE
                     TPEACAIREVNEETGLTITPPQRMCFTNDIFPEEQKHYITLFLTADHIEGEAENKEPE
                     VCETLEWFEWDNLPTPLFLPLVNLHEAGHNPFTI"
     gene            complement(4279..4923)
                     /locus_tag="VGE59_03250"
     CDS             complement(4279..4923)
                     /locus_tag="VGE59_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY1074686.1"
                     /translation="MPDSTHTNTKIVIFDFDGTIADSLDILVKIINKQAHRYGYKKIT
                     ETTINRFRDENSLTILRDMKLPLLRILFLARHVKKEFQQSIEEVKPVYGMHKTLRTLK
                     DQGYTLGIATSNSKVTVEQFLKQHDLDIFDFVIGKTVFNKGKMLAGILRHFRFPLANV
                     FYVGDETRDIEAARHANIKVISVTWGINSGELLSTYNPDFIIDKPEQLTEVVKG"
     gene            complement(4992..5222)
                     /locus_tag="VGE59_03255"
     CDS             complement(4992..5222)
                     /locus_tag="VGE59_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074687.1"
                     /translation="MAVTDDIYRMRSHISDMRNEANQLHYDAITRADEARQRVLDQFN
                     NKIEELKKQIDEEEKRVNDLEKANNDVNNLEV"
     gene            complement(5427..5501)
                     /locus_tag="VGE59_03260"
     tRNA            complement(5427..5501)
                     /locus_tag="VGE59_03260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5466..5468),aa:Gly,seq:tcc)
BASE COUNT         1515 a         1181 c         1327 g         1602 t
ORIGIN      
        1 ttaggcctgt ctggcgcggt tatggtttat ggtcgtgatt taccaagcga tcagactctg
       61 acccaatgga agcccgctga ggcaactcgt atttacgatc gcaacggtac tctcttgtat
      121 gagctctacg gtaacgaaaa aagggttgtt gtttcgggag acgacatttc gccttacctc
      181 cgtttcgcta cagtggcaat tgaagatcaa cgattttatg atcactacgg aattgatcct
      241 cgtggaattc tgcgggctgc tgtccgtaat gcacagcacg ataatcgtac agagggcggc
      301 tcgactataa cccaacagct cgcaaagaac gtttatcttt ctcctcgacg tactttcgat
      361 cgcaagattc gtgaggcgat gttagcgatc aaattggaac agcactatac aaaagatcaa
      421 attttaactt tatatcttaa tcaagttggg tatggcagta atgcgtacgg tgcggaggct
      481 gcttctcaaa tgtacttcgg taaaccggcc aaggatctca ctttagcaga agcggcaact
      541 ttggcggcgt tgcctaagtc acctggcaca ctctctcctt actcgtctac aaccgaccgt
      601 ctcattgctc gccgtaatac ggtaataaaa aagatggttg aaatgcacta tttgcctcct
      661 caagaagcag ttctcgctac tcaacagaag ttggcagtta agccgcgtga agaaaaaatt
      721 acagctcctc attttgtgat gtatgtgaag gagaagttaa ttgagaagta tggtgaagag
      781 atggtattaa atggcggcct gcaggtgacg actaccttgg atttatcaaa gcaacagcta
      841 gcggagaaaa ttgcaaacga gtcgactgct catttgtctc gggcgggtgc tacaaatatg
      901 gggttagtcg ctgtggagcc caaaactggc gaaattctag caatgttagg aagtaaagat
      961 tactttgatg tcgctcatga tgggaatgta aatgtgacca taagcagtag gcctccggga
     1021 agttctttta agccggtcgt ctatgcaggt cttatggaaa aaaatgggtg gggtccaggt
     1081 agtactttgt ttgatgttga aacagatttt ggagacgcta gtttcccata tgtgcccaat
     1141 aactatgatg agaaatatca tggcccggtt tctattcgcg aagcactcgc taactcctac
     1201 aatgtacctg cagttaaagc gatgtcttta atagggaaag aagacactat ctcgttggct
     1261 cagcgattag ggatcacaac ccttactgac cctaatcgct atggattatc tttagttctc
     1321 ggtggaggag atattcgtct tctggagtta gcgggtgctt acacgtcgtt cgctaacgaa
     1381 ggcgcatact cccaaccggt tgcaatccga aaggtggtac aaggtgacca tgtactattt
     1441 gaacactatt ccgaaccaaa gccagttttt cgtcccgagg tagcatacga ggtgagctct
     1501 atcctgtctg accctgttgc tcgctctcga gtattcggta caagaacacc tttgaatatt
     1561 gcaggacata ctgtggcggt aaaaacgggc acaacgcaag aatatcggga tgcttggact
     1621 gtagggtaca ccccttcgct cgtcgtggga gtgtgggtgg gtaataacga ttatcatcct
     1681 atgaagtccg gctctgctgg tgcaatggct gccgctcccc tctggaatac ttttgtttca
     1741 caagcattag ctgggactcc taacgaagac ttcgtagctc cttctggtat tcagatagca
     1801 accatcgatg ctattacggg gaaattgccg accgatataa cacgagaaac tcggcaagat
     1861 ttgttcgctt cctggcaatt acctggacag gttgaacttg cctcattcca aatctacgac
     1921 tgtaatggac aggttaaagc gacaaagcga agttatgtgg ttacttcgga gagaccgaat
     1981 gatccaaact gggaaaagcc agtcttggaa tgggcccgca cgcatggcta tccggtgcga
     2041 catcagggag atattcatga gccatgtcca actccctctg tggaagtcgt ggaagtacaa
     2101 aatcctgaag aggccgcact tggggggatt cctgcggagc tgtcaccaac atttgtaatc
     2161 gagtctactc caaagccgac gccggaagtc caggtaaccc cgacgccacg cccttctgcc
     2221 gaagagccaa caccttcgcc ttctccgacg gtagaaccta cggataatca atcacgcact
     2281 ccttccactc ggaccagccg tcgaagggat tagaagttaa tctgcgtccg gctcacttac
     2341 gcaatggtgt tgctggttaa gtttgtcctt tggaaagtat gaaaggaaag taacaatggc
     2401 aaagacagaa aaaccattat tccaatcttt tttttgggga ggctttgagt gtgctagcca
     2461 cattggtctt actcgaacgc gttgggactt gttaggtatg agtcgccacg atattctatg
     2521 ccgtgaagat tatcagcgtc ttaaggagct tgggattaca accgtacgtg aaggcctggc
     2581 ttggtcccag attgatttag gcaagaacaa atatgacttc tcccgctatg aatccatgat
     2641 gaaagttgcc cacgaagaag ggatagaaca agtatgggat ttaaatcatt ttgactatcc
     2701 agaatatctt gatcctttta gtgaacggtt tgttatacaa tttgctcgct acgcgcaaaa
     2761 agcaattaag ctcattcgta aatataaccc caaccggacg ctctacattg tccctattaa
     2821 tgagatctca ttctggtctc atatgggggg aatgattgga gtctgggcac cgcataaact
     2881 tacgcgtggc tatcaattta agcagcagct tgtgcgggct tctatcgcag ctatggatgc
     2941 aatatgggag atcgattcga atgtgcgttt tattcaagta gatcctattt ttatgcggac
     3001 ggctaaaaag ccgatcagca ttgtaaaaaa ggcaaccgaa gattcatttc gagagataaa
     3061 atttcaagcc tttgatatgc tatgcggaaa gttaaaacct gagcttggcg gacatccaag
     3121 atatctcgat attataggtg cgaactatta tctttataat caggagtgga ttacaggtga
     3181 tgacccgctc gacgattctt gtcacgagtt aataccctgg aatagtcgtg cccgtgtttc
     3241 attagctaca atgttacttg aagtatatag ccgttaccac cgccctattg ttgtgacaga
     3301 aactggtgcg tggggagatt ggcgagaaaa gtggtggaaa cgaacgttaa aagaaattga
     3361 cgatgcctta atcgaaggcc ttccactcta tggactgtgc gcttacccag ttattgatcg
     3421 gcatgattgg gatgatgctc accttacaaa ctccggatta tgggattttg aggagggtga
     3481 ttcacgttgt cgacgaattc cccatgaagc gagcctccaa gctatccgcc catatataga
     3541 aaagtggcat aaattaacat caacgggtta aaagctgtgg actttatcct attaaaaaca
     3601 gaaaacgact catttctgag tcgttttctg atggggcggc caacggggat caaacccgtg
     3661 acaacagggc cacaacctgt cgtgttatca ctacaccatg gccgccatgt gatattggcg
     3721 tccccagcac gactcgaacg tgcaacctac tccttagaag ggagttgctc tatccgttga
     3781 gctatgggga cgtgttggtc tggaattcct aactagtata cctgtttttt gcctaggggt
     3841 aaagagaatg acggtgccgt ttgccctaga tagtaaaagg attgtgacca gcttcgtgca
     3901 aatttaccaa gggtaagaag agtggggtag gaagattgtc ccactcaaac cattctaacg
     3961 tttcacatac ttcgggctct ttattctctg cttccccttc gatgtggtct gccgtaagaa
     4021 agagggtaat ataatgcttc tgttcttcgg gaaagatatc gttggtaaag cacatgcgtt
     4081 gaggaggggt aatagtcagt cctgtttctt cgttgacttc tctaatggcg caagcttcgg
     4141 gtgtttcgcc aaattctaaa tgccctccaa ctaatcccca ggttccctct ccgtgtgatc
     4201 cccggcgttt aattagaagt actttatttt cttttcgaat aaaaactcca acaccaactt
     4261 taggacggtt ttccatggtt atccttttac tacttcggtg agttgttctg gcttatcaat
     4321 gataaagtct ggattataag tagaaagaag ctcaccgcta tttatacccc aagtcacaga
     4381 gatgactttt atgttagcat ggcgggcggc ttcgatgtct cgggtctcat ctcctacgta
     4441 aaacacattt gctaagggga accgaaagtg ccgtagaata cccgctagca tttttccttt
     4501 gttgaagact gttttcccga ttacgaaatc aaaaatgtct aaatcatgtt gtttgagaaa
     4561 ttgttccacg gttacttttg agttggatgt ggcaattccg agggtgtaac cttggtcttt
     4621 gagggttcga agggttttgt gcatgccata gacaggtttt acttcttcaa tagactgttg
     4681 aaattccttt tttacgtgac gggcgagaaa caaaattcgt aataggggta atttcatatc
     4741 tcgtaagata gtgagagagt tttcgtctcg gaagcggtta atcgttgtct cggtaatctt
     4801 tttatagccg tagcgatgag cttgtttatt aataattttg accaatatat caagtgagtc
     4861 agcaatagta ccgtcgaaat cgaagataac aattttagta ttagtgtgag tagagtcagg
     4921 cacctttcct ttatttcaag aaaatccctg actgtaaaga cagggatttt ctaagtcggg
     4981 ggctgatctt tttatacttc tagattgttt acgtcattat ttgctttttc aagatcatta
     5041 acgcgttttt cttcttcgtc aatctgtttc tttagttctt cgattttatt attaaactga
     5101 tcaagtactc gttgtcgtgc ttcgtcggcc cgagtaattg catcgtaatg taattggttc
     5161 gcttcgttac gcatatcgct aatatgggaa cgcattcgat aaatatcatc ggtaactgcc
     5221 atagaaactc ctttctccat cttcagagtt gagcagtatg tctgctcaac tcgttaatag
     5281 tatgggaggg acaccaactt gataatatct ttgctggtag tgaaaaaata gggaaagaat
     5341 accaatgaag tgcggaggga tatcgagcgt tttgtgaatg ttatgaaaag ataaacaaaa
     5401 aacacccctt tagggtgtct tttgtctgga gcgggcaacg ggaatcgaac ccgtgtcctc
     5461 tgcttggaag gcagatataa tagccattat acgatgcccg caactgcatg cattatatct
     5521 aaaaaaagtg tcaaagacaa cttggtttat ttgcatcatg tgcgcatatt actataatag
     5581 gataaatgga gggcgcatat tatgcatcag aaaaattact tactc
//
LOCUS       DASWYB010000058         6641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_950891, whole genome shotgun sequence.
ACCESSION   DASWYB010000058 DASWYB010000000
VERSION     DASWYB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950891"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VGE59_03265"
     CDS             <1..614
                     /locus_tag="VGE59_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY1074688.1"
                     /translation="ESHLASTLYLVMGSVTYLVVLIPTLLYLLLSGVLTQQEIYAGGV
                     VIIGSFVVILEFIRFFKKQGIIYWALSRTHDDIPAFIDTWKDHQYDITALRQAFVFSL
                     GVQFLGMVVLAISLAAFGVENSIFLAVLAYVVTAVVLTLSPVFQGIGLVEFSMVYTLT
                     QFGVAKNSALAITLLYRVFQLWLPLIMGGGIIVWTRLLRLKKN"
     gene            complement(611..2191)
                     /locus_tag="VGE59_03270"
     CDS             complement(611..2191)
                     /locus_tag="VGE59_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY1074689.1"
                     /translation="MWHHFSKGQKTALGIAGILFAVLLSTYTYSFLQPGIKADVMSSN
                     CNVPLSAKVTAPTEGQIFIKTSSTLNIALAGEVKGTQGHTINTVQFALYDQSSISSAP
                     FSRLSATTGATGAGGLTTYSATFSNAQIGSYRVNMEVDYDNSMRLVSPDVSFTVSATT
                     PSPSPSPSTTPTIRPTSTPTITPSPSPTPTLLPTSTPSPKATTSPRVSWLQNWLFPTA
                     QAQIPSAGRLCITVTQAGSSTPISGATVLYLVNSGNTNTLGSYQDITNNQGYVELVGL
                     RIAPPYPTVGTVTITKQGYASVTLSGVELNQNSNVPYATELKAEMTAVTPSPLACTIL
                     TSPEQQQVNDAIQSVKDTIITSDRSGPIGPLFEQKLAQAQSTANVNQGITKLIQGLRS
                     ELKTTLSDNEEKLAKSAFNEFIADTAILNGIGKHGDSESNEKKKTIKDIDLSRSFSMR
                     DGLTYRLKIKLQGDRLDLGLNGGVGCNPLQGSISAKVRLDLTYSIIVEKERAAGVFVK
                     VDSDKGIYNIGGAFTQRF"
     gene            2301..3554
                     /locus_tag="VGE59_03275"
     CDS             2301..3554
                     /locus_tag="VGE59_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HEY1074690.1"
                     /translation="MVVADLEIHSRFARACSKTLNPQELNDWAKRKGVNLLSTGDITH
                     PLWRREFRNAVDERETGFFVLKSEKNDFLAPRFILGGEVSLMFREGEKKGRRVHLLFL
                     APSFEAVERLVAKINHKGKLASDGRVILSMSCREFALACQEADPHIIVIPAHIWTPWF
                     GLLGSKSGFDSIEEAFGDQASYIRTYETGLSADPAMCWRVETLNSRVLISSSDAHSAP
                     NMMREATLFNGNITDYSYARLYDVLIEVGNKDYYGTLEFYPQEGKYHADGHAACKLTF
                     DPAMTKTLKGRCPKCGGLITVGVNHRVDDLASHPVGYVPKHAGKVLYSVPLQEIIAEA
                     VAKGKSTKSVQSLYFQMISEIASEYSLLHEVESFPEWVPEKVVEGIYMVRRGEVNVIP
                     GYDGVYGVVKLFSSGDSKVTRWGYS"
     gene            3551..4540
                     /locus_tag="VGE59_03280"
     CDS             3551..4540
                     /locus_tag="VGE59_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEY1074691.1"
                     /translation="MKEKRIVCLGGGTGLSTLLRGLKRYCEPIAVVTMADSGGHSGRL
                     RDELGVLPPGDVRACLVALADDDKATLLRDLFNYRFMNGSYNGANVGNLLLAALSDIL
                     GDFNKAVGAAHDLLDLKGQVLPVTLEPVDLLAELADGTILFGEKAIDLPRTNGHVSIK
                     RVWLQPKVKVNPAVVKALLDADAIVLGPGDLYTSLLPNLHVEGVVEAIRQSGAKLIYL
                     ANIMTKHGETNGYAVEDYVVTLERELPRPIDIVVYNTKPIPDSLQKRYEIEKALPVRA
                     RADRHNWHGVDVLSTAGDLARHDSEKLAAILMSVLETDPEEYLAIPLITEQTV"
     gene            4548..5402
                     /gene="mutM"
                     /locus_tag="VGE59_03285"
     CDS             4548..5402
                     /gene="mutM"
                     /locus_tag="VGE59_03285"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY1074692.1"
                     /translation="MPELPEVEIIRQGLARYVPGKTIASLEVRVPKLFTGKIEDVEGQ
                     SVIGIRRRAKVLMIDLSNDKTILVHLKMTGQLVFQSADEHEKVVGGHPQEAYNQPLPH
                     KHSHVIFTFTDGSKLFFNDLRKFGWLQLLDTNVAGEYGMLATVGPEPLGGAFTESVFR
                     ERLSKYPNRLIFQSLLDQSLVAGIGNIYANEALYEAGILPERRVKDITDGEWSKLYRA
                     VRHVLEESIKYGGTSDNTYIQVDGKRGDYLAHAHVYHKKIANPCGHEVVRKKLGGRTT
                     HFCPIDQT"
     gene            complement(5404..6075)
                     /locus_tag="VGE59_03290"
     CDS             complement(5404..6075)
                     /locus_tag="VGE59_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074693.1"
                     /translation="MNELLHSTPSLETKEPSPEYTPSFSESIKLEPLPIENFELTEDE
                     QAWWSAYISAQEALMELTPEQHVYADFLAEKQGLKMIQEVRDFKSTPEAVSPGVIYLD
                     TTYDIAAVTAGSKPAALLTHSQLGTIGQRLITIGQESGLDVANITTYAGPSIIFGTAA
                     AVQTITDLYDTYLADNRFPDALFDETLGRALGYPEEAISQYVARRTEIGRYDEFAAKK
                     FEVVN"
     gene            complement(6087..6278)
                     /locus_tag="VGE59_03295"
     CDS             complement(6087..6278)
                     /locus_tag="VGE59_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074694.1"
                     /translation="MTIQEPLMSQGLTSATSRQKKKKTHPNCIIFFFLNLNPPHPIDA
                     SLKAAMSNPGPLAAFASAL"
BASE COUNT         1627 a         1333 c         1748 g         1933 t
ORIGIN      
        1 atgagagtca cttagcttcc acgctctatt tagtgatggg atcggtaacc tatcttgttg
       61 tcctgattcc tacgctttta tatcttctgt tgagcggtgt gctcacgcag caagagatat
      121 acgctggtgg tgtcgttatt attggaagtt ttgtggtcat tctcgagttt attcggttct
      181 ttaaaaaaca ggggattatc tattgggctt taagtcgaac tcatgacgat attccagctt
      241 ttatcgatac ctggaaagat catcagtacg atataacagc tttgcgtcag gcctttgtgt
      301 tttcgttggg tgtgcagttt ttaggcatgg tagtgcttgc tattagcctc gctgcttttg
      361 gggtagaaaa ctcaatcttt ttagcagttt tagcttatgt cgtgacagcg gtggttctta
      421 ctctttcgcc cgtttttcag gggattggac tcgttgagtt ttctatggtc tatacactta
      481 cacaatttgg ggtggcgaaa aacagcgctt tagcaataac gttactctat cgtgtatttc
      541 agctatggtt gccgcttatt atgggcggag gaataattgt ctggactcgg ttgcttcgac
      601 tgaagaagaa ttagaaacgt tgggtgaatg caccaccaat gttataaatt cctttatctg
      661 aatcaacttt tacaaagact cccgcggcac gttctttttc cacaataatc gagtatgtca
      721 ggtcgagtcg gactttggct gagatagagc cttgaagtgg gttacatccg accccgccgt
      781 ttaacccaag gtctaaacgg tcgccttgta gtttgatttt caggcgatac gttaacccat
      841 ctcgcattga gaaagatcgg cttaagtcga tatctttaat tgttttcttt ttttcattac
      901 tttcgctatc gccatgtttg ccaatgccgt taaggattgc cgtatcagca ataaattcgt
      961 tgaaagctga tttagctagc ttttcttcgt tgtcacttag tgtggttttg agttcagagc
     1021 gtaacccttg aataagcttg gttattcctt gattgacgtt ggcagtagac tgggcttgag
     1081 ctagtttttg ttcgaagaga gggccaatgg ggcctgatcg atctgaagtg atgatagtat
     1141 ctttcactga ctggatagca tcgttcacct gttgttgctc gggagatgtc aggatcgtgc
     1201 aggcgagcgg tgaaggtgtg actgccgtca tttctgcttt gagttcagtg gcatagggta
     1261 cgttactatt ttgattgagt tccacacccg agagggtaac gctggcataa ccttgtttgg
     1321 taatagtcac ggtaccgact gttgggtaag gcggcgcaat tcggaggccg acgagctcca
     1381 cgtatccttg attgttggta atgtcttgat aactccctaa tgtatttgtg ttaccactat
     1441 ttacaaggta gaggacggtg gcgccgctta taggggttga tgatccagct tgagttactg
     1501 taatacaaag ccttccggct gaaggaattt gagcctgtgc agtaggaaat agccagtttt
     1561 gtagccacga aactctcgga gaagtcgtcg ccttcggact tggagttgat gttggaagaa
     1621 gggtcggcgt gggagatgga gaaggcgtaa tagttggggt ggaggttggt cggatggttg
     1681 gtgttgtaga tggggaaggt gacgggcttg gtgttgtggc cgaaactgta aagctaacgt
     1741 cgggggaaac gagccgcatt gaattatcgt aatcaacttc catattgaca cgatatgagc
     1801 caatttgagc attgctaaat gttgccgaat acgtagtaag tccacctgcc ccggttgctc
     1861 cggtggtagc ggataagcga gagaacggtg cactacttat actactctgg tcgtaaagag
     1921 cgaattgtac tgtgttaata gtatggcctt gtgtcccctt tacttcacct gctaaagcaa
     1981 tattaagtgt gctgcttgtc tttataaaaa tctgaccttc agtaggcgct gtcacttttg
     2041 cagaaagggg aacattgcag ttactgctca taacatcggc tttaatccca ggttggagga
     2101 aagaataggt ataagtagag agaagtaccg cgaatagtat tcccgcaata cccagagcgg
     2161 ttttttgtcc tttagaaaag tgatgccaca taaatccctc gtatttataa tgtgtagtta
     2221 ttatttactg tatcatggaa gcggacttgg caatatatgt tgttgcgccc tattagcggg
     2281 tctgttacag tgcctgcagt atggttgttg ctgatttaga gattcactcg cgttttgctc
     2341 gagcatgtag caaaacgctt aatcctcaag aactcaatga ttgggcgaag cgcaaaggcg
     2401 taaatctttt gagtacaggc gatattaccc atccgctgtg gcgccgggaa ttccgtaacg
     2461 ctgttgatga gcgagagaca ggtttttttg tccttaaaag cgagaagaat gactttttgg
     2521 caccgcggtt tattttggga ggtgaagttt cgcttatgtt ccgggagggt gaaaagaaag
     2581 gccgacgggt ccaccttctt tttctcgctc cttcgtttga ggctgttgag aggctggtgg
     2641 ctaaaatcaa tcataaaggg aagcttgcca gcgacggccg ggtaatttta agcatgtcat
     2701 gccgtgaatt tgcgctggcc tgccaggaag cagaccctca cattattgtt ataccggctc
     2761 atatctggac gccgtggttt gggttacttg ggagtaagtc ggggtttgat tctatcgaag
     2821 aagcttttgg cgatcaggca agttatattc gcacctacga aacaggtctt tcggccgatc
     2881 ctgccatgtg ttggcgagtc gaaacattga actcccgggt gttaatttcg agctctgacg
     2941 ctcactcggc tccgaacatg atgcgtgaag caaccttgtt taacggaaac ataacggact
     3001 actcttatgc tcggctttat gatgttctga tagaagttgg taataaagat tactacggta
     3061 ccttggaatt ttatccacaa gaagggaagt atcatgccga tgggcacgcc gcgtgtaagc
     3121 ttacctttga ccctgcaatg actaaaacat tgaaaggacg ctgtccaaaa tgtggtgggc
     3181 ttataacggt gggagttaac caccgcgtgg atgatttggc cagtcatcct gttgggtatg
     3241 tgccaaaaca cgcggggaag gtactttact cggttcccct ccaagaaata attgccgaag
     3301 cagtggccaa aggtaagtca acgaaatctg tccaatcact ctattttcaa atgatcagcg
     3361 aaatagcgtc ggaatattca ttgctacatg aagtagagtc ttttcctgaa tgggtgccgg
     3421 aaaaggttgt tgaaggaatt tatatggttc gtcgcgggga agtgaacgtc attcctggct
     3481 atgatggtgt ctatggggtg gtcaagcttt tttcctccgg agactcaaag gtaactcgat
     3541 gggggtattc atgaaagaga agcgaattgt ctgtttaggc ggtggtacgg ggctctctac
     3601 tttattacgc ggtttaaagc gatactgcga accaattgca gttgtgacga tggccgattc
     3661 gggcggacat tccggtcgct tacgagatga gttaggcgtt cttccgccag gcgatgttcg
     3721 agcctgttta gtagcattag ctgatgatga caaagccacc ctgttgcgtg atctctttaa
     3781 ttatcggttt atgaatggat cgtataatgg ggcgaatgta gggaatcttt tgctcgccgc
     3841 gttgagcgat attttgggtg actttaataa agccgtggga gcggcccatg atttgctcga
     3901 cttaaaggga caagttttac cagttacact tgaaccagtt gatttgttgg cagagctcgc
     3961 cgatggcaca attttatttg gtgagaaggc gattgattta ccacggacga atggccacgt
     4021 gagtatcaaa cgggtttggc ttcagccaaa agtaaaagtt aatccggctg ttgtaaaagc
     4081 tttactggat gcagacgcta tagtgcttgg tccgggcgac ttatatacgt cgcttttacc
     4141 gaatttgcat gttgaaggtg tggtagaggc gattcggcag tctggcgcca agttaatcta
     4201 tctcgccaat attatgacca aacacggcga aaccaatggc tacgcagttg aagattatgt
     4261 cgttactctg gagcgagagc tcccccggcc aattgatata gtggtataca atacaaaacc
     4321 gattcctgat tcactacaaa aacggtacga aatagagaaa gctttacctg ttagggctcg
     4381 cgccgatcgt cataactggc atggggttga tgtgctctcg acagccgggg acttggcccg
     4441 tcatgatagt gaaaaattag cggccatact gatgtctgtt ttagagaccg atccagaaga
     4501 gtatttagct attcccctga taaccgaaca aacggtataa taaggctatg ccagaattac
     4561 cagaagtcga aattatccgc caaggccttg ctcgttatgt accgggcaag acgattgctt
     4621 cgctcgaagt tcgtgtcccc aagttattta ccgggaaaat tgaagacgtg gaaggccaat
     4681 cagtgatcgg tattcgtcgc cgcgcgaagg tactcatgat tgatctgagc aacgacaaaa
     4741 ctatccttgt tcatctcaag atgacagggc aacttgtgtt tcagtcggcc gatgaacacg
     4801 aaaaagtcgt tggtggtcac ccccaagaag cgtataacca gccattaccg cataagcaca
     4861 gccacgttat ttttactttc accgatggca gtaaactctt ttttaatgat ttacgtaaat
     4921 ttggatggtt acaactttta gatactaatg tcgcaggtga gtacggcatg ttagcgacgg
     4981 ttgggccaga accgcttggc ggtgctttta ctgagtcagt ctttcgtgag cgattgagca
     5041 agtatcccaa ccgccttatt tttcaatcac ttttagacca aagtttagtg gcggggatcg
     5101 ggaatatcta cgccaacgaa gcgttatatg aagcgggaat tctccccgag cgccgagtta
     5161 aagatattac ggacggcgag tggtctaagc tttatagagc agttcgacat gtgcttgaag
     5221 aaagtattaa gtatggtggt acgagcgata acacctatat tcaggttgat ggcaagcggg
     5281 gggattattt agcccatgcc cacgtttacc acaagaagat agcgaatcct tgtggccatg
     5341 aggtggttcg taaaaagtta ggtggccgta cgacacattt ttgtccgatt gatcaaacgt
     5401 aggttagttt accacttcga atttttttgc agcgaattcg tcatatcttc cgatttccgt
     5461 tcgtcgtgca acgtattgag atattgcttc ttcaggatag ccaagtgctc ttccgagtgt
     5521 ttcgtcaaat aatgcgtcag ggaatctatt atcggccaga tatgtatcat ataaatcagt
     5581 aatagtttga actgctgcgg cggtgccaaa aataattgat ggtccggcgt aagtggtaat
     5641 attggcaacg tccaatcctg attcttggcc aatagtaatg aggcgttggc caatggtgcc
     5701 gagttggctg tgcgtgagga gcgcagcagg tttactccct gctgtaaccg cggcaatatc
     5761 gtaagttgtg tctaggtaga tgactcctgg gctcacggcc tccggggtgg atttgaagtc
     5821 acgtacttcc tgtatcattt ttaatccctg tttttctgct aagaaatcag cgtatacatg
     5881 ctgctcgggg gtaagttcca taagcgcttc ttgggctgat atgtaggcgc tccaccacgc
     5941 ttgttcgtct tctgttagct caaagttctc tatcggtagg ggttctaatt tgattgattc
     6001 cgagaaggaa ggcgtatatt caggagaagg ttctttggtc tcgagtgaag gagtagaatg
     6061 taggagttcg ttcatatgtc tctattctac agagctgaag caaaagctgc tagcggtccg
     6121 ggattagaca tagcggcctt aagagatgca tcaatcggat gcggaggatt gaggttcaga
     6181 aagaagaaga taatacagtt cgggtgggtt ttcttctttt tctgtcggct ggttgcgctg
     6241 gttaacccct ggctcatgag aggttcttga atcgtcaagg cggactgggc ataacctctt
     6301 gcattctcga gagaagttgc ctacaatatc actatgtcga tccagacagc ttccatgact
     6361 atcaagcaga acaagggcct cctcgtcctt cttcttggtg ttgtcaatcg ggagctataa
     6421 tcgactagaa ctgtattctg ctacatcgtc gacgagggct cccctaaagg gagccctctt
     6481 tcgtgaggag agatcaatga gtggcgtaag gggaatggag agcacatccg ttgattctac
     6541 cccagcagga tggccgcaaa tcggtgagat cgtcgaacgg ggcaggacat caacgggtaa
     6601 accgtacgtt gttgtctgca gtggaactgt caccgaaggt g
//
LOCUS       DASWYB010000059         6475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_287988, whole genome shotgun sequence.
ACCESSION   DASWYB010000059 DASWYB010000000
VERSION     DASWYB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287988"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2103
                     /locus_tag="VGE59_03300"
     CDS             <1..2103
                     /locus_tag="VGE59_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1074695.1"
                     /translation="IIVAEALLSQVDATAKVLKTFPGSELIGATYESPYQLSLEEGVD
                     RVVFGEHVTAESGTGIASIAPAYGEVDSQVGKRESLPMVQTIRPDGTLKSGMNLPGEG
                     LFMKEANKAIISDLDQRGFLVRQEVIHHTYPFCWRCDSPLLYFPTTSWYIAVTKIKDA
                     LVAENEKINWLPTHLRDGRFGNWLEGARDWAVSRDRFWGAPMPIWECPDCDKIKVVHS
                     IKDIEVDNAFDLHRPKIDKVTLPCACGGTMKRVPFVFDCWFESGSMPYAQHHYPFEHS
                     DQFNPERGTGYPADFIAEALDQTRGWFYTLHVLGVALFGQRAYKNVVTSGLILASDGK
                     KLSKRLRNYTDPEVVFSTIGADALRLFLFTAASIGEDYRFSDAAVNDVKRRWITPLLN
                     SVQYYHLSLPQCEKDKAPSAEHALLDEWIKARVAQARYQVTEYMDGDDTHSPFDLARA
                     CRVFGPLVEDLSTWYIRLSRGRTDQAFTETLQAVLVKISTTFAPFLPFLTEHIYQAVK
                     LSDPKFPESVHLCTLRSLDEWKNESIINEMELARKVVSLGRDARVKVGIPVRQPLRSL
                     IVTMQADAELNLSEPVKDLVAQELQVDKVVVEKNASSDSMIMVTLDSTLTEDLRNRGM
                     ANLLRRTVQDLRKQAALQPGDAAVVSIPELDDSVSMLLRDQLGQTELVQEAYSGEVIG
                     HTEITEGERSLDITLYSR"
     gene            2113..3219
                     /gene="rodA"
                     /locus_tag="VGE59_03305"
     CDS             2113..3219
                     /gene="rodA"
                     /locus_tag="VGE59_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY1074696.1"
                     /translation="MFRFKTFDWSLVIIPLLLTTISIATIYTITFVTVGNKLAISQLI
                     YTLIGFGLLGLFSFLDYRQFKSMSVAIFIIGLLLLVPMVPIWSHKLPFVRCDFNACRW
                     LDLGFFQFQTSELFKVIIVLTMGAFLVDRTGKMPWWQLFLYLGLLAIPVGLIMEQPDL
                     GTSIVVAFCGVILLLSARFPMWIWVALVVTGLLAMPVVWQNLKPYQKKRIEVFLNPES
                     DINRTGYNVRQAEIAVGSGGVTGRGFGQGSQSQLNFLPVAHTDFIFAGYAEATGYIGS
                     VFLVIVYLFLIWRALHVAEISKDMYGRFVALGIAAMFAIQVLINVGMNIRLMPVTGVP
                     LPLVSYGGTSLFVTMIALGILQSIVIRHKKISFS"
     gene            3322..3636
                     /gene="rplU"
                     /locus_tag="VGE59_03310"
     CDS             3322..3636
                     /gene="rplU"
                     /locus_tag="VGE59_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY1074697.1"
                     /translation="MVAIIETGGKQYCVQPGQKLEVERLTASVGETVSFANLLVAGSD
                     VAVEVLKHKLGDKVITRKFRNKTRNNKVRGHRQTLTLIQVKNETAHKAASASKSKTSE
                     AA"
     gene            3643..3849
                     /gene="secE"
                     /locus_tag="VGE59_03315"
     CDS             3643..3849
                     /gene="secE"
                     /locus_tag="VGE59_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY1074698.1"
                     /translation="MKRLATPFRLVVQYLKGAYQELRQVSWPSRQAVLQYSLLVIVTI
                     VISVAVLTVFDYGLQQLANRYLLS"
     gene            3870..4520
                     /gene="nusG"
                     /locus_tag="VGE59_03320"
     CDS             3870..4520
                     /gene="nusG"
                     /locus_tag="VGE59_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY1074699.1"
                     /translation="MSDTKPFLSINPEEPQQPVEEAGVTVSSTADASSEEKLTKQSGQ
                     RGWYIIHSNTGYEDQVAQNLRQRIESFGMQDKIFNVIVPKEKEIEIRGGKRRTVEKKI
                     FPSYVMVEMIVTDESWYVVRNTPNVTGFIGLGVRPTPMSQQELDRIRKRMGVEEPKYK
                     IELAEGDLVQIKDGPLKGFEGKVTEVDSDKGRVKVLVSMFGRETPVTLDFLQVSKV"
     gene            4535..4957
                     /gene="rplK"
                     /locus_tag="VGE59_03325"
     CDS             4535..4957
                     /gene="rplK"
                     /locus_tag="VGE59_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY1074700.1"
                     /translation="MAKAIKQVIKVQAPAGKAVPGQALGPVLGAAGINIMEFINQFNE
                     RTREMGNTIIPAVITVYEDRTWDFVTKTPPVPELIKKSLGIEKGAGNVKKQKVGKLTN
                     DQIRDIAQTKIQDLNARNLESAEAMVRGTAKSMGVEVE"
     gene            complement(5041..5352)
                     /locus_tag="VGE59_03330"
     CDS             complement(5041..5352)
                     /locus_tag="VGE59_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074701.1"
                     /translation="MKQPLFNWIVYEIQTESSVINGVMLRGRIRKLGLEKGFNVLVEN
                     AQDQENTVRFATLSKEDAETISTYIHSVINDASVSLVLESLANPVLSKLKVNIETRYN
                     L"
     gene            complement(5379..>6475)
                     /locus_tag="VGE59_03335"
     CDS             complement(5379..>6475)
                     /locus_tag="VGE59_03335"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY1074702.1"
                     /translation="YITHTDFYDSHPPLGKLIIAASITLFGNVPLGWRFFNAIAGILL
                     LGVIYGFTKDLTKNRGIAVLALFLVAIEPMALVESRVGLINIYLALFSLAGLWFFWRW
                     WDKPKHHISDYILALIFFAAAASVKWIGLGAIGAALAFLVLAGWMRTENRAKFNFWHI
                     ALLAIIPLLYIASFTVDLIQTRSPLLEHFIWWHKSTWNYHAHLDATHPYGSSWWSWAI
                     MLRPIWLYYQSPTPGTILGIVEVGNIVTWIGGLIALFATMFTLGYDRKNLERNVFLIL
                     TYLALYLPWIFISRVKFIYHYFVPVLLLLILLATVTYERLLKKPEYRPIGIIFLTLGF
                     AFFVYFLPLLMGLPLSDEWYRHHMWFRSWI"
BASE COUNT         1771 a         1332 c         1617 g         1755 t
ORIGIN      
        1 atcattgttg ccgaagcact tctctcacaa gttgacgcta cggccaaagt gctcaaaact
       61 tttccgggga gtgagctgat tggggcaacc tatgaatcgc cttaccaatt aagcctagaa
      121 gagggagttg atcgggttgt ctttggtgag cacgttactg ccgaaagcgg aacggggatt
      181 gccagtattg cgcctgctta cggggaagtt gactcgcaag ttggcaagcg tgaatcgtta
      241 cccatggttc agacgattcg gcctgatggt acgcttaaat caggtatgaa cttacccggt
      301 gaaggcttgt ttatgaagga agctaataag gctattatta gtgatctcga tcaacgtgga
      361 tttcttgttc gccaagaagt aattcatcat acttatccgt tttgctggcg ttgcgattct
      421 cctctcttat actttccaac gacttcttgg tacattgccg taactaaaat aaaagatgcc
      481 ttagtggctg agaacgagaa aatcaattgg cttcctactc acttacgtga cggccgtttt
      541 ggcaattggc ttgaaggagc tcgtgattgg gcggtgtcgc gcgaccgttt ttggggcgcg
      601 ccaatgccaa tttgggaatg tccggattgt gacaagataa aagttgttca ctcgataaaa
      661 gatattgaag tagataatgc ttttgatttg catcgcccga aaattgataa agtaactctt
      721 ccttgcgcat gtggtggaac tatgaagcga gtaccctttg tcttcgactg ttggtttgaa
      781 agtgggtcaa tgccgtacgc gcagcatcac tatccctttg aacatagcga tcaatttaat
      841 cccgaacgtg gtacaggcta cccggcggac tttatcgccg aagcgttaga tcagacccgc
      901 gggtggttct atacattgca tgttttgggc gtggcattgt ttggccagcg tgcttataag
      961 aacgtggtga caagcggtct tattctggca tcagacggca agaagctttc aaaacgtctg
     1021 cggaactaca cggatcccga agtagtattc tccacgatag gagctgacgc attgcgcctg
     1081 tttttgttca cggcggcaag cattggtgaa gattaccgtt tttccgatgc cgcggtgaat
     1141 gatgtcaaac gacggtggat tacgccgctc ttaaactcgg ttcagtatta tcatctaagc
     1201 ctcccgcaat gtgagaaaga caaggcacca tcggccgaac atgctctctt ggatgagtgg
     1261 atcaaagctc gggtagctca agcccggtac caagtcacgg aatatatgga tggggatgac
     1321 acccatagtc catttgatct cgctcgagcc tgccgtgttt ttgggccact ggtggaagat
     1381 ctctcgacat ggtacatccg tctctctcgt ggtcgaactg accaagcctt tacggagacg
     1441 cttcaagccg tattagtaaa aatcagtacg acctttgcgc catttttacc gttccttacg
     1501 gagcatatct accaagcggt aaagctttct gatccaaaat ttccagagtc agttcatctc
     1561 tgtacgcttc gttcgttgga tgaatggaaa aacgaatcaa tcatcaatga gatggagctt
     1621 gctcgtaaag ttgtttctct tggacgagat gcacgggtaa aagtaggtat tccggtccgt
     1681 cagccacttc gctccctcat tgtaacgatg caagcagatg cagaattaaa cctttctgaa
     1741 ccagttaaag acctggttgc gcaagaatta caggtagata aagttgtagt agaaaaaaac
     1801 gcttcttccg atagcatgat aatggttacc cttgattcca cgttgacgga agatcttcgt
     1861 aatcgtggaa tggcgaactt actacgtcgc acggttcaag atttgcgaaa acaagcggcg
     1921 ctacagcctg gtgatgctgc agttgtttcg attcctgaat tagatgattc ggtgagcatg
     1981 ttgcttcgag atcagctcgg acaaacggag ttggtgcaag aagcatattc aggcgaagtc
     2041 atcggccaca cggaaataac tgaaggggag cgatctctcg acattacgct ctattcgcgt
     2101 taaaaaagaa gcatgtttcg atttaagact tttgactggt cactcgtcat tattccgttg
     2161 cttctcacaa caattagcat tgccacgatc tatacgatta cctttgtaac ggtagggaat
     2221 aaattagcga ttagtcagct tatttatacg ctgattgggt ttgggttgct cggtttgttt
     2281 agctttctcg attaccggca gtttaagagt atgtcggtgg cgatctttat tatcggcctg
     2341 ctactcttgg tgccgatggt gccgatatgg tcacataagc tgccgtttgt tcggtgcgat
     2401 tttaatgcct gtcgttggtt agatttaggc ttttttcaat ttcaaacatc cgagctattc
     2461 aaagttatta ttgttttaac gatgggagcg ttcttagtcg ataggacggg aaaaatgcca
     2521 tggtggcagt tgtttctcta tctcgggctt ttagcgattc ctgtgggact tattatggag
     2581 cagccagatt taggaacctc aatcgtggtt gctttttgtg gtgttattct gcttctctcc
     2641 gctcggtttc ccatgtggat atgggttgcc cttgtggtga ctggactcct ggcgatgcca
     2701 gtcgtatggc aaaaccttaa accttaccaa aagaaacgga tcgaagtatt cttgaatcct
     2761 gaaagcgata ttaaccgaac tggctataac gttcgacagg ctgaaattgc cgtgggcagt
     2821 gggggtgtta cgggtcgggg gtttggacag ggaagtcaga gtcagttaaa ctttttgcct
     2881 gtggcgcata cagactttat ttttgctgga tatgcggagg caacggggta tattgggtcg
     2941 gtttttctag tcatagtgta tctattctta atttggcgcg ccttgcatgt ggcagagatc
     3001 tctaaagata tgtacggacg atttgttgct cttgggattg cagccatgtt tgccattcaa
     3061 gttcttatta atgtggggat gaatattcgt ttaatgccgg taacgggagt gcctttacct
     3121 ctcgtttcgt acgggggaac atcgttgttt gtgacgatga ttgcacttgg tattttacaa
     3181 agtatcgtta tccgtcataa aaaaataagt tttagttaat gcgctcataa aattatcctc
     3241 cagggggagc ataattcagt tgacggagac ccgttactct ggtacaattt acatgttaca
     3301 aataagaaaa ggtaaatagc tatggttgcg attattgaaa caggtggcaa gcaatattgt
     3361 gtccagcctg gtcagaaatt agaagtcgaa cgactcaccg cttctgttgg agaaacggtt
     3421 tcttttgcca accttctcgt cgctggctca gacgtcgcgg ttgaagtgtt aaaacacaaa
     3481 cttggcgata aggttattac ccgtaagttt cgcaataaaa cccgcaataa caaggtccgt
     3541 ggtcatcgtc agacattgac gcttattcag gttaaaaacg aaactgccca caaagctgct
     3601 tcagcttcaa aaagcaaaac atcagaagct gcctaagtgt ttatgaagcg tcttgctacg
     3661 ccattccgtt tggttgtaca gtatctgaaa ggggcgtacc aagaattgcg acaggtctca
     3721 tggccgtcgc gtcaagcagt gcttcagtat tcgctgcttg tgattgtaac gattgtgatt
     3781 agcgtcgcag tgctcactgt gttcgactat ggtctccagc aattggccaa tcgttacctc
     3841 ctgagctaat cgtcccaata aggagtcgta tgagtgacac caagcctttc ctcagcatta
     3901 atccagaaga accccagcag ccagtagaag aagctggtgt tacggtttct tctactgcag
     3961 acgcatcgtc tgaagaaaaa ctgaccaaac agagtggtca gcgtggttgg tatatcattc
     4021 actcaaacac cggttatgag gaccaagttg ctcagaacct tcgccagcgt attgaatcat
     4081 ttggcatgca agacaaaatt tttaatgtga tcgtgccaaa agaaaaagag atcgaaattc
     4141 gcggtggaaa gcgtcgcacg gttgaaaaga agatcttccc cagctatgtc atggtggaaa
     4201 tgatcgttac tgatgaatct tggtatgttg tgcgaaatac gcctaacgta acgggcttca
     4261 ttggtctggg tgtgcgtcct accccaatgt cgcaacaaga gctcgaccgt attcgtaaac
     4321 gaatgggtgt agaagagcca aaatacaaga tcgaattagc agaaggggat ctcgttcaaa
     4381 ttaaagacgg tcctctcaag ggctttgaag gtaaagttac cgaagtggat tccgacaaag
     4441 ggcgcgtcaa agtgctcgtc tcgatgtttg gccgtgagac acccgttact ctcgacttct
     4501 tgcaagtatc taaggtttaa atttaaggaa acatatggca aaggcaatta aacaagttat
     4561 taaagtgcag gctcctgccg gcaaagccgt accaggtcaa gcgttgggtc ctgttcttgg
     4621 tgctgctggt atcaatatta tggaattcat taaccagttt aatgaacgca cccgcgaaat
     4681 gggtaacacc attattccag ccgttattac cgtctacgaa gaccgtacct gggattttgt
     4741 caccaagacg cctcctgtcc cagagctcat taaaaagtct ctcggtatcg aaaaaggtgc
     4801 aggaaatgtc aaaaagcaaa aagttgggaa gttaaccaac gatcagatcc gcgatattgc
     4861 acaaacaaag atccaggatc ttaacgcgcg aaaccttgaa tcagcagaag ctatggtccg
     4921 cggcactgcc aaatcaatgg gcgtcgaagt agagtaagtt cgctttaaag agcaacaaat
     4981 aaggcactga cgaaagtcgg tgccttattt gttgcttaat gtttttaaaa ggaaggagga
     5041 ttataggtta tatctagttt ctatatttac tttgagctta cttagaacag ggtttgcaag
     5101 gctctctagc acaagagaga ccgaggcatc gttaatgacg ctatggatat aggtggagat
     5161 agtctcagca tcctctttgc tcagagttgc aaacctgaca gtattctctt ggtcttgggc
     5221 attctcaact aaaacgttaa agcccttttc aaggccaagc tttcgaattc taccgcgcaa
     5281 catcacaccg ttaattacag aggactctgt ttgtatctcg tatactatcc agttgaaaag
     5341 tggctgtttc atagagtgct acaggagata tggcgttttt aaatccaact tctaaaccac
     5401 atatgatgac ggtaccattc gtcggataga ggaagcccca tgagaagagg gaggaaatag
     5461 acgaagaaag caaagccgag ggtaaggaag atgatgccga ttggacggta ctcgggcttc
     5521 tttaataatc tctcataggt aacggttgcg agaaggatga gtagcagaag gacgggcaca
     5581 aaatagtgat aaatgaattt tacgcgagaa atgaatatcc aggggaggta gagggcaagg
     5641 taggtaagga tgagaaaaac gttgcgttct aagtttttac ggtcataacc gagagtaaac
     5701 atagttgcaa agagagcaat aagtcccccg atccacgtta cgatatttcc cacctcaacg
     5761 atgccaagaa tggttcctgg agtaggggat tggtagtaga gccaaatcgg tcggagcata
     5821 atagcccagg accaccaaga agatccatag ggatgggtcg cgtcaagatg ggcgtgatag
     5881 ttccaggtgc tcttgtgcca ccaaataaaa tgttctagta aggggctgcg cgtttgaatg
     5941 aggtcaactg taaacgacgc tatataaagg aggggaataa tcgctaagag ggctatgtgc
     6001 cagaaattaa atttggctcg attttctgtc cgcatccaac ctgccaaaac gagaaaggct
     6061 aatgctgctc ctattgcgcc tagtccaatc cattttacag aagcagcggc ggcaaagaaa
     6121 ataagagcaa ggatgtaatc gcttatgtgg tgttttggtt tatcccacca gcgccagaaa
     6181 aaccagaggc cagctaagga aaagagagcg agataaatgt taataagacc tactcgggat
     6241 tcgactaagg ccataggctc tatagcaacc agaaaaagag cgagaacggc gattcctcgg
     6301 ttttttgtta agtctttggt aaagccgtaa atgacgccaa ggaggagaat gccggcgatg
     6361 gcattaaaga atctccaccc caaagggacg ttaccaaaga gagtaatgct tgcggcaata
     6421 atgagtttgc ccagtggggg atgagaatca taaaaatcag tatgggtaat ataat
//
LOCUS       DASWYB010000060         6808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_454437, whole genome shotgun sequence.
ACCESSION   DASWYB010000060 DASWYB010000000
VERSION     DASWYB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454437"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VGE59_03340"
     CDS             <1..320
                     /locus_tag="VGE59_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY1074703.1"
                     /translation="YMAYFDKRISAPMLLDMMAKFTDRTGYFEVATAYAKPSGEVKEY
                     SFKVPFTLATEERGELQKGWSRLIILEGESRTLAEYPETDRIHIWNKNYLAIARDILS
                     ETS"
     gene            complement(304..960)
                     /locus_tag="VGE59_03345"
     CDS             complement(304..960)
                     /locus_tag="VGE59_03345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1074704.1"
                     /translation="MTNEERFWNDPLIVKEFAEYPVPPYWAEYFSRQPKNLKILDLGC
                     GGGRNTSYLIQEGFDVWACDVHAGMVEETCARVGSFLETDKANQRVFQSNMTDLPFDA
                     DTFDCVLANGVYHNVSSLKEFTQAIRETSRVLKPDGILCVNVFCDEHVAADLTPYGNE
                     IHMYQTAQHLPMALLPAELILEQLATFNLTPTTAVARYESPVNTGVRSVLRGTFMKSR
                     "
     gene            complement(957..1964)
                     /locus_tag="VGE59_03350"
     CDS             complement(957..1964)
                     /locus_tag="VGE59_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074705.1"
                     /translation="MFSAVTIRYPSRLNAMAIDPSKIAMEASLRYSPGEVIFSVALFR
                     TVQLHVRRDSQIVISPTSKRPVLIKHAVEIMRQALGVTHGIDVGFTDETDIRHCGLGS
                     SSGTIAAVATAMNELYGNPIPSTDLLRFLAQNHGEEIDTMPGYLYPVQCIGGSAAAGL
                     CRGGIIVLAGESVPIGIADVSGDYEAVIGIPNDVPDYDAKTLMDKEIESLPKFIETGV
                     KYGGENAYNVLHKMLPAMHLHDLATIGDVIYEYRFRMGSIENCSFVYPRLPALCARLA
                     PLKHSGIADVLAVSSVGPGIFAITRDPQKCEAAFKQEGLTVYRTKVHNQGYEVTEKVQ
                     T"
     gene            complement(2056..2649)
                     /locus_tag="VGE59_03355"
     CDS             complement(2056..2649)
                     /locus_tag="VGE59_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074706.1"
                     /translation="MIFIVGPHGAGKTKAAHIIAKYGLASIDLGPTLRKIHRDTASKL
                     SFEEWVTQGEGEYGVNFTDALLVREIAALQGGLLHDGYQGDLLILGSRSYPGIEYICA
                     HTAPMNGFPCRILFIEASIEVMRTRFNAREGKGLSVEKFQKYIQRPSQQGIAGIRPYA
                     HPLWNDGTEQQLELALLEYLRESTHVLPFNEKKFITV"
     gene            2805..3281
                     /locus_tag="VGE59_03360"
     CDS             2805..3281
                     /locus_tag="VGE59_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY1074707.1"
                     /translation="MLISRAGYQTTLELIVDLKRQLSDVGKEAGAAFQDAGQGHHDNW
                     AWENATQERSKVEGRILELEHQLEVVTYIDDLPMNAEAVCPGHVVQLRDEHGEEAEYT
                     LLGSLEVDLDNDIISVESPLGKALLGHAPGDTVAYNCPAGILCVTILSLERSPLLQ"
     gene            3473..4033
                     /locus_tag="VGE59_03365"
     CDS             3473..4033
                     /locus_tag="VGE59_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY1074708.1"
                     /translation="MYSPDGTRTIHHIRESVIPISLQLITGLFLINGLYAVILAYLLL
                     PEINVVQNEPVLSIAFLLACHAIAFILQVVVSLKLLLPWIGRNYYIAGNHLIRTQGVI
                     VADEDVFDLHSLRSVHIHENWLGRLLNYGDIHILVAASGYRAEVIINAITNPREYERI
                     FRKFLEGSDQPLVQDEIVIDSVPSKR"
     gene            complement(4056..4658)
                     /locus_tag="VGE59_03370"
     CDS             complement(4056..4658)
                     /locus_tag="VGE59_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY1074709.1"
                     /translation="MKTILLTSSGLSSNPVRKAFIEVMKDRPKGPVALVTTASPRKEK
                     SPWTQMAQQEFLDLGFEQVDFVDIKAEPKFDFSPYQIIFVCGGNTFYLQKYSRLAGFE
                     ATVRMHLANGGTYMGESAGSIIATPTTRLANTVAPDPNEVKTVNFTGFKLVPFEIFPH
                     YEKQYEAELQAYEQEHGVKVERLTDDQALFILDDSVIRIG"
     gene            4751..5251
                     /locus_tag="VGE59_03375"
     CDS             4751..5251
                     /locus_tag="VGE59_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY1074710.1"
                     /translation="MQGRVIAICIGPERGKPMQSVSEVEAIAGAGLKGDRYATGTGSF
                     SRGEIGKRQVTLMNAIFFPGSGYEYPDSRRNLFVEGVELMWLVGREFQIGEATFKGVK
                     YCEPCRRPTKLGGDKFQGVQPFNEAFFDRGGLLAEVVQGGTIRVGSVVIPPPKNYKEE
                     EEENEK"
     gene            5414..5959
                     /locus_tag="VGE59_03380"
     CDS             5414..5959
                     /locus_tag="VGE59_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HEY1074711.1"
                     /translation="MQTEWILGSESQRKIDTATRVIAQVSGQAGFTVKGCAAESGVGN
                     TPYDEDTPRGAQNRASDSKRHFPQADYWIGLESGIVTRYGKTFEETWAVVLTKDEKAF
                     FGFSSGLVVPNVVLKRMQETDQDHWQVMPQLRAEHGIENDKDTWGVYSGYTILREISL
                     EEALRNALVQIFAPKQAFYHL"
     gene            complement(6008..6736)
                     /locus_tag="VGE59_03385"
     CDS             complement(6008..6736)
                     /locus_tag="VGE59_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY1074712.1"
                     /translation="MKINLDLRFLCVVLLIAFLVALWGWKPWSASTQTITVSGEATIS
                     AAPDEFVFSPTYQKRAAAVTDAISQVSELGNGVVAKLKEMGVAEEKIKTSVTSQQYYE
                     PTTGKVTNEFAANYAVTATVNDKEVAKKILEYVATTPTLYGVSPQSTFTKETRKKLES
                     EARGKGLADARAKAKQTAKELGVKLGRVATVTEGSGFGGPIIYGYDAYATKPAATSQS
                     TTPPVLQTGTEDINFTVSVVYKIR"
BASE COUNT         1838 a         1760 c         1563 g         1647 t
ORIGIN      
        1 cctatatggc ttactttgac aagcggattt ccgctccaat gctccttgat atgatggcaa
       61 agttcacaga ccgtaccggc tattttgaag tagcaaccgc ctacgcaaag ccatcaggtg
      121 aagttaaaga atattcattc aaagtaccgt ttacccttgc aaccgaagaa cgcggtgagc
      181 tgcaaaaagg ttggagccga ttaattatac tcgaaggaga aagccgaaca cttgccgaat
      241 atcccgagac tgaccgtatc catatttgga ataaaaacta cctcgccatt gcccgtgaca
      301 tacttagcga gacttcataa acgtaccgcg taagacagac cgcactcctg tattaacagg
      361 actctcgtaa cgagcaaccg cagtcgtagg tgttaaatta aaagtggcca gttgttctag
      421 aattaattca gcggggagta aagccattgg tagatgttga gcagtctgat acatgtgtat
      481 ttcattgcca tatggagtga gatcggccgc aacatgctca tcacaaaaga cattcacaca
      541 aagtatgccg tctggcttaa gaacgcgtga agtttccctg atcgcctgtg taaactcctt
      601 aagcgatgac acgttatgat acacgccatt ggcaagaacg caatcaaatg tatccgcatc
      661 aaaaggcaga tcggtcatat tagattgaaa aacacgctga tttgccttat ctgtctctag
      721 aaacgaccct acacgtgcac acgtctcttc aaccatgccc gcatgaacgt cgcacgccca
      781 cacatcaaaa ccttcctgga ttagataaga agtatttcgg ccaccaccac aaccaagatc
      841 taatattttt aaattcttag gctgcctcga aaaatactcg gcccagtaag gcggtactgg
      901 atactcggca aactctttca caataagggg gtcattccaa aatcgctctt cgttagtcat
      961 gtttgtactt tttccgtaac ttcgtacccc tgattatgaa ctttcgtgcg gtaaaccgta
     1021 agcccttctt gcttaaatgc cgcctcacat ttttggggat ctctagttat cgcaaatata
     1081 cccggcccta ccgaagaaac ggccaaaaca tcagcaatcc cagaatgctt gagaggcgcc
     1141 agccgggcac agagagcagg cagcctcgga tagacaaatg aacagttttc aatactcccc
     1201 attcggaatc gatactcgta aattacgtcg ccgattgtgg ccaggtcgtg caggtgcatc
     1261 gccggtaaca ttttatggag cacattatag gcgttttcgc caccatactt tacgcctgtc
     1321 tcaataaact tcggaagcga ttctatctct ttatccatga gcgtttttgc gtcataatcc
     1381 ggaacatcgt tggggatccc aatcaccgct tcataatcgc ccgacacatc agcaatacca
     1441 atcggaacac tttcaccagc caagacaata atccctcctc ggcataaacc tgctgcagca
     1501 gagccaccaa tacattgcac cggatagaga tatccaggca ttgtgtcaat ttcttcacca
     1561 tgattctgcg ccagaaaacg cagtagatcc gtagatggga taggattacc ataaagttcg
     1621 ttcatagccg tagcaacggc cgcgatcgta ccgctacttg atccaagccc acagtgccgg
     1681 atatcagttt cgtctgtaaa accaacatca atcccatgcg taacaccaag tgcttgacgc
     1741 ataatttcca ctgcatgctt gatcaacact ggccgtttag aggtaggaga tatgacgatc
     1801 tgagaatcac gccttacgtg aagttgaacc gtgcgaaaga gagccacgct aaaaataacc
     1861 tcacccggtg aataacggag cgacgcctcc atggcaatct tgcttgggtc aatcgccatc
     1921 gcgtttagtc ggcttgggta ccgaatcgta acagcgctaa acaagggttc aataggttga
     1981 aggagacgat gatagggatt aaaaactgtc cccgcttgaa acggggacaa cgcagacggc
     2041 ggctgagccg acgactcaga ctgtgataaa tttcttttcg ttgaaaggta ggacatgtgt
     2101 tgactcgcgc aaatactcta acaacgcgag ctccagttgt tgttcggtgc cgtcattcca
     2161 gagtgggtgg gcgtaaggac gaatgccggc tatcccctgt tggcttggac gctgaatgta
     2221 cttttggaat ttttcgaccg aaagaccctt cccttcacgc gcgttaaacc gtgtgcgcat
     2281 cacttcgatc gaggcttcga taaagagaat ccggcagggg aacccattca tcggcgcagt
     2341 atgcgcacaa atatactcaa tccccgggta tgatcggctg cccaagatga gaagatcacc
     2401 ctgatatccg tcatgaagaa ggccaccttg taatgcagca atttcgcgaa cgagtagcgc
     2461 gtcagtaaag ttaactccgt attctccctc accctgcgtc acccattcct caaacgacag
     2521 cttggaagcc gtgtcccgat ggattttccg gagagttgga ccaagatcaa tcgaagcgag
     2581 tccatacttt gcaatgatgt gcgcagcttt tgttttacct gcgccatgtg ggccaacgat
     2641 aaagatcatg acacacctcg gtttagcagt agtagactaa cttacttgcc gttgattata
     2701 atctgctttc tgggtcatgt caaagagtca ataaccgatt gacagtattg accttattcg
     2761 ctattatcag gtcatcgatt tcggtcgtca aaaggcaggg ggaaatgtta atctcgagag
     2821 cagggtacca gactaccctg gaactgatcg tggaccttaa aaggcagctc agcgacgtag
     2881 ggaaagaagc aggagcagcc tttcaagatg ccggacaggg gcatcatgac aactgggcgt
     2941 gggaaaacgc cacccaggaa agatccaaag tagaaggtcg aatactggaa ctagaacacc
     3001 aactcgaagt ggtaacgtac atcgatgatc tcccgatgaa tgcggaagcc gtatgtcccg
     3061 gccacgtcgt gcaattgcga gacgagcatg gagaagaggc cgaatacacg ctcctcggct
     3121 cgctcgaagt agatctcgac aacgacatca tctccgtcga gagtccgctt ggaaaggctc
     3181 tgctcggcca tgcccccggc gacacggttg cctacaactg cccggccggg atactctgcg
     3241 taacgatcct gtctctagag cggtctccac tgctccagta acaaaacaaa ccaaaataat
     3301 acgtcaaagt aaatcgaagt tccgatcctt gggtggcatg ccaccctttt tcttttctgt
     3361 cctttttata aaaagtcact cgtgaacagg aatgacactc ttcgtaattc gcttcttttc
     3421 gctcaaaaag tgatatactt acattactgt taaaactaag aaaggtggca ccgtgtattc
     3481 accagacgga actcgcacca ttcaccatat tcgggaaagc gttatcccca tctcgctcca
     3541 gctcattacg ggactctttc tcattaatgg cctctacgct gttatattgg cctacctcct
     3601 tttgcccgaa ataaatgtcg tccaaaacga acccgtactt agcattgcct tccttctcgc
     3661 ctgtcacgct attgcgttca ttttacaggt ggtagtatcg ctaaaacttt tactcccctg
     3721 gatcggccgt aactactata ttgccgggaa ccaccttatc cgaacccaag gagttattgt
     3781 tgccgacgaa gatgtctttg acctccatag cttacgttcc gtccacatcc acgaaaactg
     3841 gttgggaaga ctcttaaact acggggatat ccatatttta gttgccgcgt cagggtaccg
     3901 cgcagaagtc attattaacg ccatcacaaa tccacgagaa tacgagcgta tttttcgtaa
     3961 attcttggaa gggtcggatc aacctcttgt ccaagatgaa atcgttattg attctgtccc
     4021 aagcaaacgg taaccgttac cggatggaga ggaacttacc ctatacgaat aactgaatcg
     4081 tcgagaataa agagcgcttg gtcgtcggta agtcgttcta cctttacacc gtgttcttgt
     4141 tcataggctt ggagctcggc ttcatactgc ttttcgtagt gcggaaaaat ctcaaacgga
     4201 accagtttaa acccagtaaa attaaccgtc tttacttcgt taggatcagg agcaacggtg
     4261 tttgccagcc gggtggttgg ggtagcaata atgctgcccg cactttcgcc catgtaagtg
     4321 ccgccatttg ccagatgcat ccgtacagtt gcctcaaacc ccgcaagccg ggaatacttt
     4381 tgtagataga acgtattgcc gccacagaca aaaataattt ggtacggcga aaaatcaaac
     4441 tttggctcgg ctttaatatc gacaaaatcg acttgttcaa aacctagatc aagaaactcc
     4501 tgttgcgcca tttgcgtcca ggggcttttc tctttacgtg ggctagctgt tgtgacgagt
     4561 gccacaggac ccttcggccg atctttcatc acttcaataa aggccttccg caccggattc
     4621 gaactcaacc ccgatgacgt taataaaata gttttcatac aacaactata ccatacatcc
     4681 agattttctc cttattctat ggtagaatag ccctggttat acaccactcg ttaccgaccg
     4741 aggatgacat atgcaaggca gagttatcgc aatatgcatt ggccccgaga gaggcaaacc
     4801 aatgcagagc gtgagtgaag tcgaggccat agcgggagct ggtctcaaag gagaccgcta
     4861 cgcaacaggc acggggagct tcagccgagg agaaatcggc aaacgccaag taaccctgat
     4921 gaacgcgatc ttcttcccgg ggagcggcta cgaatacccg gattcgcgcc ggaacctctt
     4981 cgtcgagggc gtggaactga tgtggctcgt tggccgagaa ttccaaatcg gcgaggccac
     5041 cttcaagggg gttaagtact gcgaaccatg cagacggccg actaaactgg ggggagacaa
     5101 gttccaaggc gtccaacctt ttaacgaagc cttcttcgac cggggaggac tcctcgccga
     5161 ggtcgtacaa ggcggcacca tccgggtggg cagcgtagtc atccctcccc cgaaaaacta
     5221 caaggaagag gaagaagaaa acgagaagta aatcaccacg tcggacgaac aacggccgtt
     5281 tgtaccctgc aggagcagat acaaacggcc gctttctttt tttcaataaa gaactctctc
     5341 tttaacgaca cctaaaactt aatgccccat acattatctg gcgtttactc cttcaaaccg
     5401 ctatactcag accatgcaaa cagaatggat tttagggtca gaaagccaac gaaaaataga
     5461 taccgcaacg cgggttattg cccaagtaag tgggcaggcg ggctttaccg tgaaaggctg
     5521 cgcggcggaa tccggcgtgg gtaacacccc ctacgacgaa gacacaccac gtggcgccca
     5581 gaatcgagcc agtgattcaa aacgccactt cccccaagca gattattgga ttggcctcga
     5641 gagtggcatc gttacccgct acggaaaaac cttcgaagaa acgtgggctg tggtactcac
     5701 gaaagacgag aaagcgtttt ttggcttttc cagcggcctg gttgtcccta atgtcgtcct
     5761 caaacggatg caagaaaccg accaagacca ttggcaagtc atgccacaac tacgagctga
     5821 acacggcata gaaaacgaca aagatacctg gggagtctac agcggctaca cgatcttacg
     5881 cgaaattagc ctcgaagaag cactccgtaa cgcccttgtg caaatattcg cccccaaaca
     5941 agctttttat cacctctaaa acgagctcgc cactgcgagc tcgttttagt actaccggag
     6001 aaacagttta gcgaatttta tacactacac tgacagtaaa attgatgtct tcggtacccg
     6061 tttggaggac aggcggagta gttgattgac ttgtcgcagc gggcttcgtt gcataggcat
     6121 catatccgta aatgattggg ccgccaaaac cacttccttc ggtaacggtc gcaacgcggc
     6181 caagctttac acctaactct ttagcagttt gtttcgcctt ggcacgggca tctgccaacc
     6241 ctttgccacg ggcttcggat tctaattttt tacgggtttc tttggtaaac gtgctctgcg
     6301 gggagacacc ataaagcgtt ggcgtggtgg caacatactc taagattttc ttcgccactt
     6361 ctttatcgtt caccgttgcc gttacggcgt agttagccgc aaactcattg gtgactttgc
     6421 cagtcgttgg ttcgtagtac tgctgcgagg tcacgcttgt tttgattttt tcttcggcca
     6481 cgcccatttc ctttaacttc gcgacaactc cgttcccgag ttcagaaact tgcgagatcg
     6541 catccgttac cgcggccgcc cgtttctggt aggtgggtga aaatacaaat tcatctggcg
     6601 ccgcactaat cgtggcctcg cccgacacgg ttatagtctg ggtactcgca gaccaaggct
     6661 tccaacccca caaggcaacc aggaacgcaa tcaacaaaac aacgcacaga aatcggaggt
     6721 ctaagttaat tttcatagct ttagtatagc gcactttgga tatgtgtata gcgccaagcc
     6781 ggctcgcttt ttagctgctc taagagta
//
LOCUS       DASWYB010000061        12794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_33686, whole genome shotgun sequence.
ACCESSION   DASWYB010000061 DASWYB010000000
VERSION     DASWYB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33686"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VGE59_03390"
     CDS             complement(<1..1132)
                     /locus_tag="VGE59_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY1074713.1"
                     /translation="MQRFELKAPYSPSGDQPKAIAALTAGLKENMKGQTLLGVTGSGK
                     TFTMAHIIAETQRPTLVIAHNKTLAAQLASEFRQFFPNNAVEYFVSYYDYYQPEAYMP
                     NSDTFIEKEAQINDEIDRLRHSATQSLLTRRDVIVVASVSCIYGIGKPESYGKYLLTI
                     KRGESPGKEALLSRLVELQFARNDMQLTRGSFRAKGDVIEIMPTNRELVYRIELFGDE
                     VESIRILDPITMASQETLSELVIYPAKHFMMDTNDQQRALMAIENEMVARVAELKAAG
                     KLLEAERIERRTRYDLEMMREVGYCNGIENYSRHLDGRSPGEASATLISYFPKDFLMF
                     IDESHVTVSQIGAMYAGDQARKNTLIDYGFRLPSARDNRPLTF"
     gene            1291..1674
                     /locus_tag="VGE59_03395"
     CDS             1291..1674
                     /locus_tag="VGE59_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074714.1"
                     /translation="MTGLFIQWFYIDAPKRHVASMRAIVMALFQYFSISLLSSTLFSP
                     WRHDAIDIQRLPLNRWGEAIMNNFTSRLIGLTMRMCVIGAGLVSITVFSVGALLFTFV
                     WYILPFLLVGSVFYGFGLLIGGIHV"
     gene            1667..4057
                     /locus_tag="VGE59_03400"
     CDS             1667..4057
                     /locus_tag="VGE59_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY1074715.1"
                     /translation="MSNRLRLLAQTDLFLHQVGFIRWIVRATLFILLLASVLLGDSNA
                     QALILGFFFIILPLGILSDLFSLFLKEEINIAESLQQKDPEGITYETATLLARSHGRE
                     NYLWLYLLQHPSTRFILNRLGVDRKKFYALSHPEFSFKEWNVAAHQLAHSEGVAVSPR
                     HFFEVTQKADIFVSLWKEYGITERERLEVWQWYERISSDIKQRSQTTANKIRFAGAIG
                     RDWASGYTRYLEQYAIDLTSQLQAVGAHISLVGHVDEKKRILEYLARDHTHNVLVVGE
                     EGIGKERLIYSLTNDFNSGTVPLSLRYKHIYRLETGQVVSARSQNELEMRLHAIFQEA
                     STVGNIVLVIPDFHLLVGAQHTEGMGAINASALLTSYLESPSLQVIGTMPPQAYYSYV
                     KPNAALEPHLLPVEVKEITASEALRVVQDEIFRYESTSKHIFTYQALAKLIQIAEQHI
                     HDTPYPEKALSLLDEVAGAASQEPQQLIYARDVERIVGEKLKVPIGAASTSEREVLSN
                     LENLIRARIIGQNEAVNVVANSLRRARAGLHSGKRPIGSFLFLGPTGVGKTEMAKTIA
                     ALYYKNEKAFIRMDMSEYQTPESLEKLVGTATSPGLLTTAVTDQPFSVVLLDEIEKAA
                     PSVRNLFLQILDDGRVTDGYGKQVDFTNSMVIATSNAGAQFIREAVESNTLDTTFKQR
                     LLEYLQVNGIYAPEWLNRFDAVVVFLPLSKEEIREVAKLQVAALTEQLQSHNIALTVQ
                     DDVYDFLVERGYDPQFGARPMRRAVQDTIESALAKVLLNDSTEGLKQITLTRDMLV"
     gene            4240..4443
                     /locus_tag="VGE59_03405"
     CDS             4240..4443
                     /locus_tag="VGE59_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074716.1"
                     /translation="MAYSVQSQKSGKTYYLHSKEVTLRGGRKQTIYYFATDVRDGALD
                     EVPAGYSVMENARTGLPMLRRSK"
     gene            4569..5978
                     /locus_tag="VGE59_03410"
     CDS             4569..5978
                     /locus_tag="VGE59_03410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY1074717.1"
                     /translation="MYKKSDLFYTVLHLPVDIAALVLAFVVSYNIRAEGSEIYLLPFQ
                     DYFSLLWRVIPLWIIVFALQGLYTRRYLFSSLQNLLHTIVSVLAGWAAFVVFLSFLKT
                     EQTIAFPRLLLIYILISSIVFIFGGRLFLRLIQSILRIFGIGRKRLVIMGKGKAADQM
                     ESTLTLNPDPAYNFVMSIESTEPKDVANLLKKERINTLVIADHSLSDNQILEYLTAAQ
                     NEGIEVNLVPNMFEVQASNVLFNTLGGLPLLTFRQTPLDGWGRITKRTFDIFLALIGG
                     IILLPLILLTAFIVKITSSGPIVFRQRRLGRNGKHFYIYKFRSMYLHLTGDHVKGKTE
                     EQIFADMGRQDLVEEYRTFKKVKVDPRVTPFGRLMRKTRLDELPQLWNVLKGDLSIVG
                     PRPIRDFELSQYGKWASYLLSIKPGLTGLWQVSGGNDISYEERVKLDAKYAQNWSLWQ
                     DMLIILKTGFIILKGGDGY"
     gene            5968..7074
                     /locus_tag="VGE59_03415"
     CDS             5968..7074
                     /locus_tag="VGE59_03415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1074718.1"
                     /translation="MAINPYALSSLKVALVHDPLTVPAGAEKVLYEFHKIFPHAPIYT
                     PLYRPEKFPEYRDVRVVTSSLNRFRFARNHHQLMIPLLPYFVEQLDLSEYDLILSDSS
                     SVAKGVITRPESIHICYCQTPMRWAWMPQLDPRASSSLIRRLAAHYLRLWDSATVDRV
                     DVWLANAKTSAARVKKFYKRDAAVIYPPVDIENVAVSTENDDYYLSVGRLVEQKRIDL
                     IVEAAKKTGSKVKIVGGGPLLEKLKSQAKRHKNISFLGRVSDQERDELYAHCKAFIFA
                     SEEDAGIVPIEAMAYGKPVIAFGRGGASETVLDKKTGLHFAEQTADSLALAIEEFETL
                     HFDAEYIGKHAQQFSSARFRQEITAFIQKTVESR"
     gene            7146..7757
                     /locus_tag="VGE59_03420"
     CDS             7146..7757
                     /locus_tag="VGE59_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074719.1"
                     /translation="MTEQKYIKLFVANYWKTWVLTALIGLVLGAILANQLKPSFEGAI
                     SFQLSRPQATQQATAPFYLYDGYYNEQAAILARTNFLNWVTTPRAIYDIYKGAGYEDK
                     ITSTSKTANYLKPGDTNITTSTAVLTYHMPSREAATKLGGAVVTYVANNYKVDGLMIT
                     GSDPLVLETQPKRVLTALAIAIALTFAAMFISLLLHFFRSDEK"
     gene            7791..9809
                     /gene="gyrB"
                     /locus_tag="VGE59_03425"
     CDS             7791..9809
                     /gene="gyrB"
                     /locus_tag="VGE59_03425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY1074720.1"
                     /translation="MADKTNYSAQNITVLEGLDPVRKRPGMYIGGTGVDGLHHLIWEI
                     INNSVDEAMAGYADRIEVTLYDDGSVGVLDNGRGIPVDIVAATKKSGLETVLTVLHAG
                     GKFGGGGYKVSGGLHGVGSSVVNALSTRLIAEVYKDGKIYRQEYNCGVPLKDVEPVGK
                     TDRKGTFIRFYPDASIFTTTTEFNRDTIVDRCRQQSYLTRALTIHLRDERDVLNLKRY
                     TFYFESGIEAYVRHINKNKEALFDPPFSVEKQSSDATVEVALTYTTDFNEHVYSFANN
                     IFTTEGGTHLTGFRTALTRAINDFAKKSGLMKETDGSLTGEDVREGLTAVISVKLGEP
                     QFEGQTKNKLGNPEMRSLVENATYEAYMDYLAEHPSEAKKIIEKVALAARARAAARAA
                     RETIIRKGALEGMTLPGKLTDCTNRDPSKSELYIVEGNSAGGSAIGARERTFQAILPL
                     RGKILNVERARLDRMLTSEEIKSLIIALGAGIGEDINIDKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRHFPSIITNGYLYIAQPPLFALKKGKELRYAYTDAERDKVFVEMGGQL
                     SEAASELPSETGEDESLPEMDDSEEVTVTAKKGAKINIQRYKGLGEMNAEDLWDTTMN
                     PENRILLSVTIDDAERADSIFSMLMGDEVAPRKRFIHTRAAQVKNLDI"
     gene            9819..12401
                     /gene="gyrA"
                     /locus_tag="VGE59_03430"
     CDS             9819..12401
                     /gene="gyrA"
                     /locus_tag="VGE59_03430"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY1074721.1"
                     /translation="MADETLLENLNPEESSDVEAEINHDQLRKTEFGMVQDRGLVLEM
                     EQSYLDYAMSVIVSRALPDVRDGLKPVHRRVLYAMWDNGLRYNAKFSKCAGVVGEVMK
                     KYHPHGDMAIYDTLARMAQSWSMRYRLIDGQGNFGSIDGHEPAAMRYTEARLHRISDE
                     LLRDIDKETVEFRPNYDGAYKEPKVLPANLPNLLLNGAQGIAVGMATNIPPHNLTELL
                     DGIAHLIDNPDAELDDLMQFIKGPDFPTGANIYNIADIKQAYSTGKGRIMMRATTEIV
                     EKKSGFQIIISDLPYQVNKAALITKIAELVKDKRIEGISDIRDESDRKENIRVVVELK
                     STAYPKKILNRLYDLTPLQSAFHVNMLALVNGIQPQVLTLKQVLEYFILHRVEVIRKR
                     TEYELRKARERAHILEGLLIALNDIDAVIETIRRSKDRDDARVNLISKFKLDEIQANA
                     ILDMRLSQLAALERQRIEDEYKALMQKIGELEAILASEQKIREVVKAELLETKERFGD
                     ERRTTIHPNAVGEFSAMDLIPDEQVVVVLTQGNYIKRMPITTYRSQNRGGKGVLGMTT
                     KEEDEIQHLVTTSTHDDIFFFTNTGRVFQTKVYELPSASRQAKGTPLVNVIQILNAER
                     VTAVMTKKHDDTSKKYFVFATKSGLIKRSLIEDYKNVRKTGIVAMRIDKGDELKWVHR
                     TTGNDQIFQATAKGQAISYVEEEVRPVGRSAGGVKGIALRTGDTVISMDVIDTALKSQ
                     PDLLIVLENGFGKRTSFEHFRTQHRGGLGLKCANVTDKTGDVVGVEVVADEKMDALLI
                     SRQGVVLRTPLKEIRKLGRDTQGVTLMRMEDADSVASVAILSREEPVETTTNETAVET
                     VTSE"
     gene            12506..>12794
                     /locus_tag="VGE59_03435"
     CDS             12506..>12794
                     /locus_tag="VGE59_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEY1074722.1"
                     /translation="MAGRAKKPFVSRKRRREIVEARERRETQVQAQHERTQSEGQQYI
                     RTDKTSVHIPPVATVGEFAQLLKVPVVAVIKQLMKNGIMAAINHSIDFDTMS"
BASE COUNT         3539 a         2739 c         2969 g         3547 t
ORIGIN      
        1 caaaggtaag aggtcggtta tcacgggcag agggaagtcg aaatccataa tcaataaggg
       61 tatttttgcg ggcttggtca ccagcataca tggccccaat ttgagacaca gtaacatgcg
      121 attcatcaat aaacattaaa aagtctttag gaaaataaga aataagagta gcggatgcct
      181 caccaggcga ccgtccgtca agatggcgag agtagttttc tattccatta cagtatccga
      241 cttctcgcat catttctaaa tcatagcggg tacggcgctc gatccgctct gcttcgagta
      301 gtttgccggc tgcctttaat tcagcgacgc gtgcaaccat ctcattttca atcgccatca
      361 atgcacgttg ctggtcattt gtatccatca tgaagtgttt ggctggatag ataacaagtt
      421 cagatagtgt ctcctggctt gccatagtaa tagggtcaag aatacgaata gattcaactt
      481 catcgccaaa cagctcgata cgatatacga gctctctatt ggttggcata atttcaataa
      541 catcaccctt tgcgcggaat gaaccacggg tgagttgcat gtcattacgg gcaaactgta
      601 actcgacaag acggctgagt aaggcttctt tgccaggtga ttcacctcgc ttaatcgtta
      661 agagatattt cccgtacgat tctggttttc caataccata gatacacgaa acagaggcta
      721 caacaattac gtctcgacgc gttaggagag actgtgttgc cgagtgacga agacggtcga
      781 tttcatcgtt aatttgggct tccttctcaa taaatgtgtc ggagtttggc atgtatgcct
      841 caggttgata ataatcgtag tacgaaacga agtattcaac cgcattatta ggaaaaaact
      901 ggcgaaattc tgaagctaac tgagcagcta atgttttgtt atgggcaatt acaagggttg
      961 ggcgttgcgt ttcggcgata atgtgagcca ttgtaaatgt ttttcccgat ccggttacgc
     1021 ctaaaagagt ctgacctttc atattctctt ttaggccagc tgtgagagct gcgatcgcct
     1081 taggctgatc gcctgatggg gagtaagggg ccttgagttc aaagcgctgc atgatagtga
     1141 ttatggtatc acgaattgag tccaactacc accattaact tcatagcaca aggaaatagc
     1201 gagtctgaga caacgttgct taaaagtgta tacaagcaat ttgagtcggg cttgggagca
     1261 tgctagacta aaaagaaggt gaggagggaa atgactggtt tgtttattca atggttctat
     1321 atcgacgctc cgaagcgaca cgttgccagc atgcgtgcaa ttgtgatggc attatttcaa
     1381 tacttttcca tttccctttt atcttcgaca ttattttcac cgtggcgtca cgacgcaatc
     1441 gatatacagc gacttcctct caaccgatgg ggggaggcaa ttatgaataa ttttacttct
     1501 cgcctcatcg gattgacgat gcggatgtgc gttatcggcg ctgggttagt ttctattacc
     1561 gtgtttagcg ttggtgccct tttgtttacc tttgtttggt atatcctgcc ctttcttctt
     1621 gttggatctg tattttatgg gtttggcctt ctgattgggg ggatacatgt ctaatcggct
     1681 tcggctgtta gcccaaactg acctttttct tcatcaggta ggatttattc gttggattgt
     1741 ccgagccacg ctatttatat tgcttcttgc gagtgtttta ctgggagatt ctaacgctca
     1801 agccctgatt ctcggtttct tctttattat tttgcctctc ggaatacttt ctgatctttt
     1861 tagcttattc ttgaaagaag aaataaatat agccgaatct ctccaacaga aggatccaga
     1921 aggaattacg tatgaaaccg cgacgttact tgcgcgatct catggaagag agaactactt
     1981 atggctctac cttttacagc acccaagtac gcggtttatc ctgaatcgct taggagtgga
     2041 ccgaaaaaag ttttatgcgc tttcccatcc tgagttttca tttaaagagt ggaatgtggc
     2101 cgctcaccag cttgcacatt cagagggagt cgctgtttcc cctcgacact tcttcgaagt
     2161 tacccaaaag gctgatattt ttgtgtctct ttggaaagag tacggtatta ctgagcgaga
     2221 acgtctagaa gtatggcagt ggtatgaacg aattagttcg gacataaagc aacgcagtca
     2281 aacaacagca aataagattc gttttgcagg agcaatcggg cgcgattggg caagtggata
     2341 tacccgatac ttagaacagt atgcaattga tctgacatcc cagttacaag cagtcggcgc
     2401 tcatattagt cttgttggtc acgttgatga gaaaaaacga atcttagaat atcttgcacg
     2461 tgatcatacg cataatgtgt tggtcgttgg ggaagaaggg attggtaaag aacgacttat
     2521 ttactctctt acgaatgact ttaactctgg cacggtacct ctctctcttc gctacaagca
     2581 tatttatcgg ttagaaactg ggcaggtggt gagtgctcgt tctcaaaacg aactagaaat
     2641 gcgcctccat gctatttttc aggaagcatc taccgtaggg aatattgtct tagttatccc
     2701 tgattttcat ttactcgtag gggcgcaaca tactgaaggc atgggagcta ttaatgcgtc
     2761 ggcacttctt acttcttacc tcgagtcacc ttcactccaa gttattggta ccatgcctcc
     2821 tcaagcgtac tactcatatg taaaaccgaa tgctgctctt gaacctcacc tgctccctgt
     2881 ggaagtaaag gagattacgg cgagtgaagc acttcgggtg gtgcaagacg aaatattccg
     2941 ttatgagtca acttcaaaac acatatttac ctatcaagct ttagccaagc tcattcagat
     3001 tgcggaacaa cacatccacg atactcctta ccctgaaaaa gctctcagct tactcgatga
     3061 agttgcggga gccgcaagtc aagagccgca acagcttatc tatgctcgtg atgtagaaag
     3121 aattgtgggt gagaaactaa aagttccgat tggagcagct tctacttctg agcgagaggt
     3181 gctcagtaat cttgaaaatc ttattcgtgc ccgtattatt ggtcaaaatg aagccgtaaa
     3241 tgtggtggcc aactcgttac gtcgtgctcg tgccggcctt cactcaggca agcgcccaat
     3301 cgggagcttt ctctttcttg ggcctactgg ggtagggaaa actgagatgg caaaaaccat
     3361 cgctgccctt tactataaga atgaaaaagc ctttattcgc atggatatga gtgagtacca
     3421 gacgcctgag tccttagaaa aattggttgg aacggctact tctccaggct tacttaccac
     3481 ggccgtaacc gatcagccct ttagtgttgt attactagat gagattgaga aagcggcacc
     3541 gagcgtacga aatctttttc tccaaatact tgacgatggt cgcgttactg atgggtatgg
     3601 taagcaagtg gattttacaa actcaatggt tatcgcaaca tcgaatgctg gcgcgcaatt
     3661 tattcgagag gcagtggaat caaataccct tgatacaacc tttaaacaac gtttacttga
     3721 gtatctccag gtgaatggca tttatgcacc agagtggctt aaccggtttg atgcagtggt
     3781 tgttttcttg ccgctcagta aagaggaaat acgtgaagtg gcaaaactgc aggtagcggc
     3841 gcttacagaa cagctccaga gccataacat tgcattgact gtccaggacg atgtgtacga
     3901 ctttttagtc gaacgtggct atgatccaca atttggagcc cggcctatgc gtcgagcggt
     3961 gcaagacacc attgaatcag ccttagcaaa agttttgctc aatgacagta ccgaggggtt
     4021 aaaacaaatt acgctcactc gcgatatgct ggtttagttg cacattatat ggttaatagg
     4081 gttttctatt gcgcaacaga gaaaacaagt tctggtagtg atgtagccaa cccatcattc
     4141 caacaggttt attcctcttg acaacaggtg tgaacctgtg gaatggtagg gttgttaagg
     4201 ggtaatccct attaacggat aggaaggagg tgagctgacg tggcctatag tgtacaatct
     4261 caaaagagtg gaaaaacata ctaccttcac tcaaaggaag taacactccg aggcggtcgc
     4321 aagcaaacca tttactactt cgcaactgat gtgcgtgatg gtgcgctcga tgaagttcca
     4381 gctggttatt ctgttatgga aaacgcccgt accggacttc caatgcttcg ccgtagtaag
     4441 tagttggtat ccccaacact tagttacaca aaaagctcca acgcctgttg agagcttttt
     4501 gtgataaagg gcaatctgct gagccctgtt ctattttccc gttcatccaa atggtataca
     4561 atttacctat gtacaaaaaa agcgatctct tttacacggt tttgcacttg cctgttgata
     4621 tagcagcact cgttttggcc tttgttgtca gttataacat ccgggccgaa ggtagtgaga
     4681 tttatttact cccgtttcaa gattatttta gcttgctgtg gcgagttatt ccgctctgga
     4741 ttatcgtctt tgctctccag ggcctgtata ctcgacgtta tctctttagt tcgctgcaaa
     4801 accttcttca tacgatcgtc tccgttctgg ctgggtgggc ggcgtttgtt gtgtttctct
     4861 cttttctcaa aactgagcaa acaattgctt ttcctcgcct cctcttaatt tacattctca
     4921 tttcaagcat tgtttttatt tttggggggc gtttgttcct tcgtttgatt caatctattc
     4981 ttcgaatatt tgggataggt cgaaaacggc tcgtaattat ggggaaaggg aaagccgccg
     5041 atcaaatgga gtctactctc acacttaatc ctgacccggc atataacttt gtcatgagca
     5101 tagaatcaac tgagccaaaa gatgttgcaa acctgcttaa aaaagagcga attaacacgc
     5161 ttgtgattgc agatcattct ctttcggata atcagattct tgaatacctc acagcggctc
     5221 agaatgaagg gatagaagtt aacctcgtac caaacatgtt tgaagtgcag gcgagtaatg
     5281 ttctattcaa cacattgggc gggttgccac tcctcacgtt tcgtcaaaca cctttggatg
     5341 gttgggggag aattactaag cgcacctttg atatcttttt ggcgctgatt ggcggcataa
     5401 ttctcttacc gcttattctg ttaacagcgt ttatcgtcaa aattacatct tcgggcccca
     5461 ttgtatttcg tcaacgtcgg cttggccgca atggtaaaca cttttatatc tataagtttc
     5521 gctctatgta tcttcatctt acgggcgacc atgtaaaagg taagactgaa gagcaaattt
     5581 ttgccgatat gggaaggcaa gatctcgtag aagagtaccg tacctttaaa aaagttaagg
     5641 ttgacccacg tgtgactcca tttggacgtc tcatgcgaaa aacgcgtctc gatgaacttc
     5701 ctcagctttg gaatgtttta aaaggggact tgagtatcgt aggcccccgg ccaattcgtg
     5761 attttgaact gagccagtat ggaaaatggg cttcgtatct cctctcgata aagcctggcc
     5821 tgacaggact atggcaggtt agcggcggta acgatatcag ttatgaagaa cgcgtaaaat
     5881 tggatgcaaa atatgcacaa aactggtctc tctggcagga tatgctgata atactgaaaa
     5941 caggatttat tattttgaaa ggtggagatg gctattaacc cgtacgcact cagttctctt
     6001 aaagtcgccc ttgttcacga tcccctaaca gtaccggcgg gagcggagaa agtcctctat
     6061 gaatttcata agatttttcc gcatgccccc atctatacac ctctatatcg accagaaaaa
     6121 tttcctgaat atcgagatgt aagagttgtc acttcgtcac ttaaccgttt tcgttttgcc
     6181 cggaatcatc accagttaat gattccatta ttgccatatt ttgttgaaca gcttgatctt
     6241 tctgaatatg atcttattct ttccgattcc tcttctgtcg caaaaggtgt tattactcgg
     6301 ccagaatcaa ttcacatttg ttactgccaa acacctatgc gttgggcgtg gatgccccag
     6361 cttgatccac gggcatcttc ctcgcttatt cgtcgtcttg cagcccacta cctacgcctc
     6421 tgggacagcg cgacagttga tcgggtggat gtatggcttg ccaatgcaaa aacatcagca
     6481 gcgcgggtaa agaagtttta taagcgagat gcggcggtca tttatccgcc cgttgatatc
     6541 gaaaacgtag ctgtttctac tgagaatgac gattactacc ttagcgtagg aagattagtt
     6601 gaacaaaaaa ggattgatct aatcgttgaa gctgcaaaaa agacgggtag caaagtaaaa
     6661 attgtagggg gtgggccctt gctggaaaaa ttaaaaagtc aagcaaaacg tcacaaaaac
     6721 attagttttc ttggacgagt gagcgaccaa gaacgtgatg agctttatgc tcattgcaaa
     6781 gcattcatct ttgccagcga ggaagatgcg ggcattgtgc cgattgaggc tatggcatac
     6841 ggtaagccag tcattgcttt tgggcgtggc ggtgcaagtg aaaccgtatt ggataaaaaa
     6901 acaggtcttc attttgctga acaaactgca gatagcttgg cgttagcgat tgaagaattt
     6961 gagacgttac atttcgacgc agagtatatc ggaaagcatg cacaacagtt ttcttcagcg
     7021 cggtttcggc aagaaatcac ggcctttatc cagaagacag ttgagtcgcg ataagactgg
     7081 ctacctaccg agacttgtta ttaacggggc gttagtgtag tgtgaaaata gggaaaagga
     7141 gtattatgac tgaacaaaag tatattaagt tgtttgtcgc aaactattgg aagacatggg
     7201 ttctgactgc gcttatcggg cttgtgcttg gcgcaatttt ggcaaatcag cttaagccaa
     7261 gtttcgaagg cgctattagc tttcagctct cccgaccgca agcgacgcaa caagcaacag
     7321 ctcctttcta tctttatgat ggttactaca atgaacaagc tgcgatcctc gcccgaacga
     7381 actttctcaa ttgggtaacg acaccacgtg caatttatga tatttacaaa ggtgcggggt
     7441 atgaagataa aattacttcg acaagtaaaa ctgctaacta cttaaaacca ggtgatacaa
     7501 acattacgac gagtaccgct gtgctcacat atcatatgcc aagccgagaa gcggcgacga
     7561 aattaggtgg tgctgttgta acttacgtcg cgaacaatta taaagtagat gggcttatga
     7621 ttacgggaag tgacccactc gtcctggaaa cacagcccaa gcgtgttctg actgctcttg
     7681 cgattgctat tgcactcaca tttgctgcta tgtttatttc tctccttctt cactttttcc
     7741 gttcagacga aaaataatag ttattatcca cgttccaagc agaggttgtt atggctgaca
     7801 aaacgaatta cagtgcccaa aatattaccg ttctcgaagg tttagatccc gtacgtaagc
     7861 gtccgggtat gtacattggt ggtaccggag ttgatggact tcaccacctt atttgggaaa
     7921 ttattaacaa ctcagttgac gaagccatgg ccggatatgc tgaccgcata gaagtgactc
     7981 tctatgatga cggctcggtc ggtgtgcttg ataatggacg tggtattccg gttgacattg
     8041 ttgctgccac aaaaaaatca gggttggaaa cggttctcac tgttctccat gcaggaggta
     8101 agtttggcgg aggcggctat aaagtttctg gtggactcca cggagtcgga agctctgttg
     8161 tgaacgctct ttcgactcgt ttgattgcgg aagtttataa agacggtaaa atttaccgcc
     8221 aggaatataa ctgtggtgta ccccttaaag atgttgagcc agttggcaaa actgaccgta
     8281 aaggaacgtt tatccgtttt tatcctgacg ccagcatttt tacaaccaca actgaattta
     8341 accgggatac gattgttgac cgttgtcgtc agcaatccta tcttactcgc gcgttaacta
     8401 tccacttgcg agatgaacgg gatgttctca atcttaaacg gtacaccttt tactttgagt
     8461 ctggtattga ggcgtatgtc cgtcatatta ataaaaacaa agaggctctc tttgatcctc
     8521 catttagcgt cgaaaaacaa tcttctgatg cgaccgtcga agtagctctt acgtatacaa
     8581 ctgactttaa cgaacatgtc tattcatttg cgaataacat ttttacaacc gaaggcggaa
     8641 ctcacctaac cggttttcgc accgctttaa cacgagcaat taatgatttt gcgaagaaga
     8701 gcggtttgat gaaagaaacg gacggctcgt taactggtga agatgtacgt gaaggattaa
     8761 cggccgttat ttcggttaaa ttaggggagc ctcagtttga agggcagacc aaaaacaaac
     8821 ttggcaatcc agaaatgcgt tctttggtcg aaaatgcgac ctatgaagca tatatggatt
     8881 acttggccga gcatccttct gaagcaaaga aaattattga aaaagttgca ttagcagctc
     8941 gtgcccgtgc cgccgcccgt gccgctcgtg aaacgatcat tcgtaaaggt gctcttgaag
     9001 gcatgacatt gccaggaaaa cttactgact gcacgaatcg cgatccatcg aaaagcgaac
     9061 tatatatcgt agaggggaat tctgcgggag gaagtgcaat tggtgcccgg gagcgtacat
     9121 tccaagcgat tttgcctctt cgaggtaaaa tcttaaatgt tgaacgtgcc cgtcttgacc
     9181 gcatgctcac ctcagaagaa atcaagtctc tgattattgc tttaggtgca ggaattggcg
     9241 aagatattaa tatcgataag ctacgttatc atcgcattat tatcatgacc gatgctgacg
     9301 ttgatggagc acacatccgc acactactgt taacattctt ttatcgtcat ttcccatcaa
     9361 ttattaccaa tggctatctt tacattgctc aaccgccact cttcgctctc aaaaagggta
     9421 aagagttacg gtacgcatat acagatgctg aacgtgataa ggtctttgtc gagatgggcg
     9481 gacagttaag tgaagcagct agtgagctgc cttctgaaac tggggaagac gaatctcttc
     9541 ccgaaatgga tgatagcgaa gaagtgaccg ttacggctaa gaaaggcgcg aagattaata
     9601 ttcagcgtta taaaggttta ggcgaaatga acgctgaaga tttatgggac acaaccatga
     9661 atccagaaaa tcgtattctt ctttcagtca ccattgacga cgctgagcgt gctgattcca
     9721 ttttctccat gctcatgggt gacgaagttg ctccgcgcaa acgctttatt cacactcgcg
     9781 ctgcacaagt taagaacctg gatatttagg agtagattat ggctgatgaa acattacttg
     9841 aaaatctaaa tcctgaggaa tcttctgatg tggaagcaga aattaaccac gatcagctaa
     9901 gaaaaactga atttggcatg gttcaagatc gtggtcttgt actcgaaatg gagcaatcgt
     9961 atcttgacta cgccatgtcc gtcattgttt cacgtgctct gccggatgtc cgagacggct
    10021 taaaacccgt acatcgccgt gtgttatatg ctatgtggga taatgggctt cgttataacg
    10081 caaagttctc caaatgtgcc ggtgtggtgg gcgaagtcat gaaaaagtat cacccccatg
    10141 gagatatggc catttatgac acgttagccc gtatggccca atcctggagc atgcgttatc
    10201 gtctgatcga tggacaagga aactttggtt cgattgacgg ccatgaaccg gccgccatgc
    10261 gttatacgga agcccgttta caccgtattt ctgatgaatt attgcgagac attgataagg
    10321 aaacagttga attccgtcct aactatgatg gtgcctacaa agagcccaaa gttcttccgg
    10381 ctaacctccc taacttactt cttaacggtg ctcagggtat tgcagtcggt atggcaacga
    10441 atattccccc tcataactta accgagctgt tagacgggat tgctcactta atcgacaacc
    10501 ctgacgctga attagatgat ttaatgcagt ttatcaaagg gcccgatttt ccaactgggg
    10561 caaatattta taatattgcc gatattaaac aagcctattc tactggtaag ggccgcatca
    10621 tgatgcgggc aaccacagaa attgttgaaa agaagtctgg attccagatt attattagtg
    10681 acctgccgta tcaggttaat aaagccgctc ttattactaa aattgccgag cttgttaaag
    10741 acaagcgaat tgaaggtatc tccgacatcc gtgatgagtc agatcgtaag gaaaatatcc
    10801 gcgttgtcgt tgaactaaaa agtacggcct atcccaaaaa gattctcaat cgtctctacg
    10861 acttaacgcc gcttcaatcg gccttccatg tcaatatgtt ggcattagta aatggtattc
    10921 agccgcaggt tctcaccctc aaacaagttc ttgagtattt catccttcac cgcgtggaag
    10981 ttatccgtaa gcggactgag tatgaattac gaaaggctcg cgaacgggcc cacatccttg
    11041 aaggcttgtt gattgcgctg aatgatattg acgcggtaat tgaaacaatt cgtcgctcga
    11101 aagatcgtga tgatgcccgt gtcaatttaa tttctaaatt taaactcgat gaaatccaag
    11161 caaacgctat tctcgacatg cgtttgtctc aattggctgc ccttgaacgc cagcgtatcg
    11221 aagatgaata taaagcctta atgcagaaaa ttggtgaact cgaagcgatc ttagccagcg
    11281 aacaaaagat tcgcgaagtg gtaaaagctg agttattaga aacaaaagaa cgctttggcg
    11341 acgaacggcg tacgacaatt catcccaacg cggtcggcga attctccgca atggacttaa
    11401 ttccggatga gcaggtagtt gttgtactaa cacaaggcaa ctatataaag cgcatgccaa
    11461 tcaccaccta tcgtagtcag aaccgtggcg gtaaaggcgt gttaggaatg acaaccaaag
    11521 aagaagatga aatccagcac ttagtaacga cttcgacaca cgatgatatc ttcttcttca
    11581 caaatactgg gcgggtattc cagacaaaag tgtatgagct gccaagtgca tcacgtcagg
    11641 caaagggaac tccgctggta aacgttatcc agatcctaaa tgctgaacgt gtgacggcag
    11701 taatgaccaa gaaacacgat gacacttcaa agaaatattt tgtcttcgct accaagagtg
    11761 gcctcattaa gcgttcgctt atagaggact ataaaaatgt tcgcaagacg ggcattgtgg
    11821 caatgcgaat tgataagggt gacgaactta aatgggtgca tcgcacgacg ggtaatgacc
    11881 aaattttcca agcgacagcg aaaggacaag ctatctccta cgtcgaagaa gaagttcgtc
    11941 cagtcggccg gtctgccggg ggtgtcaagg gtattgcact acgcacgggc gatacagtta
    12001 tttcaatgga cgtcattgat acggctctta agtcacaacc ggacttactc atcgtacttg
    12061 agaatggatt tggcaaacgg acgagcttcg agcatttccg aacacaacac cgcggtggac
    12121 ttggtcttaa atgcgcgaat gttaccgata aaacaggaga tgtcgtaggc gttgaagtcg
    12181 tagcagatga aaaaatggat gcattgctga tttctcgaca aggagttgtg cttcgaaccc
    12241 ccctcaaaga aattcgtaag cttggtcggg atacgcaagg cgtgacgctc atgagaatgg
    12301 aagacgctga cagcgttgca tcggtggcaa ttctttcccg cgaagagcca gttgagacaa
    12361 ctacgaacga aacagctgtt gaaacagtaa cgtctgagta aaaatcgtaa aagaaaaacg
    12421 cagcgagaac cggctgcgtt tttcgatggt ttgcctcgct acagcaaacc tgatagaata
    12481 gggaaactta ctggagtgta tatttatggc aggaagagcg aaaaaaccat ttgtgagccg
    12541 caagcgtcgt cgcgaaattg ttgaagcacg tgagcgccgc gaaacacaag ttcaagcaca
    12601 acatgagcgg actcagtcgg aaggtcagca gtatatccgt actgataaaa caagcgtaca
    12661 catccccccc gttgcaacgg tgggtgaatt tgcgcagctc ttaaaggtac ctgttgtcgc
    12721 tgtcatcaag caattgatga aaaacggtat tatggccgcc attaatcatt ccattgactt
    12781 tgatactatg tcaa
//
LOCUS       DASWYB010000062        12346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_33862, whole genome shotgun sequence.
ACCESSION   DASWYB010000062 DASWYB010000000
VERSION     DASWYB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33862"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGE59_03440"
     CDS             <1..334
                     /locus_tag="VGE59_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY1074723.1"
                     /translation="DIPIVAKLFDFHKTFHVYVGKFPKTDKYTIGQTIQSNTLQLIEL
                     SIACTLMDNYAKKEHLAIANGKLDLLKLLVRLAFETRALDQKAYLILEGQLQEAGRMF
                     GGWLRSLR"
     gene            389..562
                     /locus_tag="VGE59_03445"
     CDS             389..562
                     /locus_tag="VGE59_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074724.1"
                     /translation="MKIVATKHEPVVRIPLVVGIVPIVVQPGPVLVPFHVEEVRVAVR
                     IRLCEKRHPTTAP"
     gene            complement(908..2989)
                     /locus_tag="VGE59_03450"
     CDS             complement(908..2989)
                     /locus_tag="VGE59_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074725.1"
                     /translation="MVATEGHMAIESGTDFLKQKYDLHNKPEVQSAAERTEHHVDGQL
                     PQNPSERIQNYLDRFSELTNRDEPEKRQRGLEALKRVLHDKFVIKSDEIPESYWETQR
                     RMAREMGHGDIEVSEEMRRQGTEIIIADQESSLDKWVDYLASDDAIYPMWLKYFAVRG
                     VLGMSTYDKERHAFAKRDKGTTAPFPDLDREALAYVLDALEKRQGSEYAEVTEQLKQK
                     KNELKRLRGEYRTLEKQGLEAEASEITLKIEETEAQISNLVQEQERIVSGSLAIPEES
                     RSELRSLLQESDFAKLYVWALEKVTPAEENELLITDGEWVKYEQGSDATPLVESLQGH
                     GTGWCTAGESTARAQLEAGDFYVYYTYSRDELLNKRQGQPAVAHVPRAAIRMEQDRIG
                     EVRGIAAEQNLDPHIADVVRAKMQEFPDGASYEKKAYDMQMLTYIEKKTKAGHELNKD
                     DLVFLYEINSTIEGFGYDRDPRIAELRAERNPDQDAPIVFDCQPEDIAHSFEDITQDT
                     KAYIGPLVPGIFTILPENLEHIYTSFPENKIRQRTIEIGGKSGAELEQDLKTKGFKIS
                     DYARHMLRSQEFVPAERPEPANLVIATVASIGFPRGATTAQIYAKAEELGLEICPAEV
                     GPHFRLQYPEHEQPMNEWNYVAMKTIAVPGGRPGLFRVERREDGAWLSSSWDDPDGQW
                     SAGSRFVFRRK"
     gene            complement(3015..3803)
                     /locus_tag="VGE59_03455"
     CDS             complement(3015..3803)
                     /locus_tag="VGE59_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEY1074726.1"
                     /translation="MKLPKITTKQQAILLLLYKYRFLNRIQIQTFLKHKYHKRINDWL
                     RDLYEKDYVGRIYSTNFGENTKPAIYYIGSNGIRFLKTQDDCCTEIIQRLYREKSRQE
                     SFISRCILIADICLELEAKTSNILSYDLLTESNLADTESPFHFLTEFDFQLCATKYTG
                     KSKKKIRFLLSVFDTTLPRYRMRNRIKKYFEFYASNEWEDATGEAFPNIYVMCPTKAL
                     MIYCKRLTKTLLEEQDAEGLHLYFTYEGLVKKAGVTSEIWESVK"
     gene            complement(4137..5003)
                     /locus_tag="VGE59_03460"
     CDS             complement(4137..5003)
                     /locus_tag="VGE59_03460"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEY1074727.1"
                     /translation="MNKYTIKHFQADFPDDNACLTYIFRRKYGSDPVCPKCGKTGFYR
                     VAKRKCFACAWCSHQLHPTAGSIFHKSPTKLTLWFYALYLASQSKNGVSAKELERHLG
                     VTYKCAWRIAYKIRELMKQPTTQLEGTVEMDEMYVGGRKKGITGRGAVGKMPVVGVVQ
                     RGGEARAEVTVDTAVYTIKPLMRKHVKFGSELMTDTYSSYYNAKKWGYTHKQINHKKE
                     QYVKGKIHTNTIEGFWSQVKRSIDGTYHAVSPKYLQFYLDEFVYRYNHRNSAQLFDSL
                     LGRVCRLPYSKA"
     gene            5099..7675
                     /gene="mutS"
                     /locus_tag="VGE59_03465"
     CDS             5099..7675
                     /gene="mutS"
                     /locus_tag="VGE59_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY1074728.1"
                     /translation="MYKTPMLQQWHEMKEQYPDCVLFFRMGDFYEMFGDDAKIGSEVL
                     HITLTARDKGGDGKMPMCGVPFHASENYVARLVQAGYKVAICEQVSQPGKGLVKREVV
                     RVVTSGTLLRDESLTDAGSQFLISLVIDAKAVGGAAADLSTGQFFAFEFRGNPDDIVP
                     LLMSHFRPRECVVPRVTYENSNYISLIRQERSANITCAHDWLVYIKDAEKLLKNHFHV
                     QSLDTFGLSGKSFAIKGAAALLGYLRYTQKGNVEHITSIQEFHLSGHLVLDQVTLKHL
                     EVIRTDQGEVAGSLFGAVNYTQTAAGQRLLQDWLLHPVTDIQEITARQDAVTELVSRP
                     ELVQQLRDALAPMSDSERLVGRLSVGLGSPRDLRRLADTLWHIHEQVLPLLSGVKSSR
                     LQQVANPLAKELLEWTIRAREILFPDAKPTLDEGYIFQTGWNSELDVLRGLTSGNQEW
                     LTQFEEQERQRTGIPKLKVVYSRAFGYAIEITKLHKDKVPPDYAPRQTLVNAERYTTQ
                     ALVSYEQKALSAMDQMIVLEKQLFMNEVHSVVAVSKIISHLAYSIASLDVFVSFAYSA
                     RTHRYIRPVITEEAGLAIEGGRHPVIEHIVETDFIANDTRLAPAHSLQLITGPNMAGK
                     STYIRQVALIALLAHTGSFVPAQSATIGVLDRIFTRIGAHDNLRAGQSTFMVEMLETA
                     HILHNATSRSLVILDEVGRGTSPEDGFALSWAIAEALAHQSKGLVLFATHFHALKDIA
                     QHHTTVFNSHLAVAVDNDDLIFLRKVVDGPTDESYGIVVAEKAGVPKAIIKRAKQLRT
                     DQDRVGKRVVSVDQLSLLAATTHDYKSKVEDKLSQIDLSATTPLQALILLNDLQASLK
                     DT"
     gene            complement(7691..8776)
                     /locus_tag="VGE59_03470"
     CDS             complement(7691..8776)
                     /locus_tag="VGE59_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074729.1"
                     /translation="MIKQRIILLSIALLIPLIGIFAFIFSSLTPRASELSPNTLGLVT
                     NTPSPFYVSYVYSPYITIDVTGESSKFDTLSMKVAYDPDYLTYDSNYVSGFLAYKPFK
                     LAIQETNGIIDITLTKDPDQLWTRSQTFGLKFASKKVGTTGVTLLPDYVFSLGGTALT
                     NYTPAKLKTELTILPIPAPVIRVFKTQPEIIKKGEKAMLVVTDSYSTNRTITPDVGIL
                     VNNTDATYVNPEKTTTYTLTSSGLGGTTTQTYTLVVEQPPTPTSTATTLSTIPQATSK
                     SSPKVTPKSTPSPTPVIAVLTSITPSPETTSPTPTFSPANQAIQDTPSSLINRLALIT
                     CLAVAAIVAITFFVRRWYLRKQTSTFL"
     gene            complement(8810..11122)
                     /locus_tag="VGE59_03475"
     CDS             complement(8810..11122)
                     /locus_tag="VGE59_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074730.1"
                     /translation="MSHHGTPSHHISKPKHTFVALVLLLALVVVLGWYFLRFQTPSAD
                     TIYSATKTITVNNTLLEQGQNVTLERDIPTGKLQLQSYPGRLENTNTQLPLNINFPNS
                     VWDSQNNQLIILGGFLQDQGKVLPDIYTYSPSQGVVTKKNASLPALRRITSVLWDSKT
                     NKAYILGGNNLNDKGATVSLSDEILVYDPVADTLTTSTTKLPYVLKETTVASIPGTDS
                     FYLIGGETMQPNGQGVVIDTILEYKPITNAITVAPFKFFTKMSGAKALTVPSANEIHL
                     FGGYRANGITRPLILDIANKTSYDRVKSGSGPGASGAIWDESANKAYLFGNALSQILD
                     YTPSNGSWWNRLMYKEDPSLSAEGVVFDSVSKIGYFIKNHNQIWRFDLNKRSYETDTW
                     PETQSSRFTSPLNIQTKWVSTEITKDTPPGTSMTFSFSAYQNNTCASPWTENIADVPD
                     STVLCIKVKAHSDDSTITPTLSTLRITYSVPMLEVGYNLDKTILNYDDTLTATFNYTN
                     QYPEVLSNVVMSIPLPSGFDTEKLEVTIPSISAQAQSAVTKTAKFTGRIHKRATESAQ
                     RTLTQTQQGTVTFTRASGEKVTLNLDPPPRFLSTTTGFRVPLQGAAKGFVATDMKAQV
                     VAKKSGESLESGKMSDAAVTTFSTASTNSPDIEDSLLGYFSDAPIKEALATITTPVDL
                     WIKPNGYLATTIRAVTNAPEVLSFSESRAGDLNNDNTVTIRDFSMFVSKYGTAISGAH
                     ELADFTFDNKVDIKDFSIFVSNYGRSGESL"
     gene            complement(11124..11987)
                     /locus_tag="VGE59_03480"
     CDS             complement(11124..11987)
                     /locus_tag="VGE59_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEY1074731.1"
                     /translation="MRTYYCRIWLIPLFIISTVFLCTPFAFAADGSLIFSAPTSAEVG
                     QEISVIAMIQADTTIDTIGYKIKYDPALLDYTQGDDSGTAFPLAATSITADETNGLIS
                     QDRGSSASGVTGAARVITFKFMVKATGTTTVSYNAGTEVLREGNVKDFSLGSTTLSLV
                     TPTTPTNTPTPTTTAPATTTPRTTATATTTTKTNTSVQTTTKASPKATTSSSSITNLT
                     TTTTTPSSNVQTLTPSASATTTSSNDSSVTTNQGSQAGKLMAIIFGLLLILIGIIGYI
                     YWHRKQRESEY"
     gene            12099..>12346
                     /locus_tag="VGE59_03485"
     CDS             12099..>12346
                     /locus_tag="VGE59_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1074732.1"
                     /translation="MGVIHQLPEEIAIKIAAGEVVERPASVVKELVENSLDAGATSIE
                     VSLDDGGKTFIEIKDNGSGMDREDAVSCFLRHGTSKIK"
BASE COUNT         3067 a         2661 c         3139 g         3479 t
ORIGIN      
        1 cgacattccg atcgttgcga aactctttga tttccataaa acctttcacg tgtatgtagg
       61 gaagttcccg aagacagata aatataccat tggccagact atccagagca acaccttgca
      121 actcattgag ctgtcaatcg cctgtaccct catggacaac tacgccaaga aagaacattt
      181 agcaattgca aacggtaaac ttgacttact taagttgctt gttcgccttg cctttgagac
      241 ccgcgcactt gaccagaagg cctacctcat cctagaagga cagctccagg aagcaggtag
      301 aatgtttggc ggctggctcc gaagcttacg gtaataaaac tccctgccat agtctgccac
      361 agttagggca acgtatcgac agggagaaat gaaaatagtt gcgacgaaac acgaaccggt
      421 tgtccgcatt ccactggttg tcgggattgt cccaattgtt gttcaaccag gccccgtcct
      481 cgttccgttc cacgttgaag aggttcgggt tgccgttcgg atccgccttt gcgagaaacg
      541 ccatcctacc actgccccct gaacgaaacg aaggggctgt attttatttg gcatctgcat
      601 gcctcagcgt cgctcaccaa gtaatttcat ttttttggta cctatgtatc acgctattac
      661 ctgccggagc gggtaatact ctggatgtta taagccctgg atttggtagc ttgggtccgt
      721 aaacccaagc aatagtcacc tactacctgg caggactgta ccatatacgg ggtaagtcgt
      781 caaaaaaaat agtcgcgagc gcgcttcgcg cgtaatccaa aacaaaacca aaacatcgcc
      841 ttagccagca ggcatcccgt gagggaaggc gaatcaaaaa gacaaaccga ccaaacttaa
      901 aacagaatta cttgcgacga aacacgaacc ggctgcccgc actccactgg ccgtcgggat
      961 cgtcccaact gctgctcaac caggccccgt cctcgcgccg ctccacgcgg aagaggcccg
     1021 ggcggccgcc cggaaccgcg atagtcttca tggctacata gttccattca ttcatcggtt
     1081 gttcatgctc ggggtattgt aagcggaaat gcggaccgac ttcggccgga cagatttcta
     1141 agccaagttc ttcggcttta gcataaatct gggctgttgt tgctccacgc ggaaaaccaa
     1201 tcgatgcaac agtagcaata acgagattag ctggttcagg acgttcagcc ggtacgaatt
     1261 cctggctacg caacatatgg cgagcatagt ctgagatttt aaagcctttc gtttttaaat
     1321 cttgctctaa ttcagcgcca gatttaccac caatttcaat cgtccgttgg cgaatcttat
     1381 tttcagggaa ggaggtgtaa atatgctcga gattctcggg caagatggtg aagataccag
     1441 gcacaagtgg gccgatatat gccttggtat cctgagtaat gtcttcgaac gagtgggcaa
     1501 tatcttccgg ttggcagtca aagacaattg gagcgtcttg gtcaggatta cgttcggcac
     1561 gaagctcggc aattcgtgga tctcggtcgt atccaaagcc ttcaattgtg gagttgattt
     1621 cataaagaaa gaccagatcg tctttattaa gttcatgtcc ggcctttgtt ttcttttcaa
     1681 tataggtaag catttgcata tcgtaggctt ttttctcgta ggaagcgccg tctggaaatt
     1741 cttgcatctt cgcccgtacc acatcagcaa tatgggggtc taagttttgt tcggcagcaa
     1801 tgccacgaac ttcaccgatt ctgtcttgct ccatccggat agccgcccga ggcacgtgag
     1861 caacggctgg ctggccttgg cgcttattta aaagttcatc tcgtgaatag gtataataaa
     1921 cgtaaaaatc tccagcttcg agctgagctc gtgcggttga ttcgcctgcc gtacaccaac
     1981 ctgtcccgtg gccttggaga gactcaacga gcggcgttgc atctgagcct tgctcgtatt
     2041 taacccattc accgtcggta atgagcagtt cattttcttc cgccggcgtg actttttcca
     2101 acgcccaaac ataaagtttg gcaaagtcgc tctcttgcag caacgagcgc agttcactgc
     2161 gactttcttc ggggatagca aggctaccgc ttacgattct ttcttgttct tgaacaaggt
     2221 tactgatctg tgcttctgtt tcctcaattt tgagagtaat ctcgcttgct tcagcttcaa
     2281 gcccctgctt ctcgagtgtt cggtattcgc ctcgcaagcg cttgagttca ttctttttct
     2341 gctttagctg ttcggttact tcagcatatt cagagccttg tcgtttctcc agtgcatcga
     2401 gaacgtaggc taaggcttca cggtcaaggt cgggaaaggg agctgtcgtt cccttatcgc
     2461 gtttggcaaa ggcgtggcgt tctttatcat aggtgctcat ccctagtacg ccgcgcacgg
     2521 caaaatactt gagccacatg ggatagatgg catcatcgga agctaaatag tcaacccatt
     2581 tatctaaact tgattcttgg tcggcgatga tgatctcggt accttgccgt cgcatttcct
     2641 cacttacttc gatatcgcca tgccccatct cacgagccat cctgcgttgc gtttcccagt
     2701 agctttctgg aatttcatca cttttgatga cgaacttatc atggagaacg cgcttgagtg
     2761 cttcgaggcc acgttggcgt ttttccggct cgtcacggtt ggtaagctcg ctgaaacgat
     2821 cgagataatt ctggatccgt tcgctgggat tctggggaag ctgaccatct acatggtgtt
     2881 ccgtccgctc tgcagcactt tgaacctccg gcttgttatg caagtcgtat ttttgcttaa
     2941 gaaaatctgt tcctgattca atagccatat gtccctctgt tgctaccata ctaacacact
     3001 aataaatgcg ttggctactt gacggattcc caaatctcgc tagtgacgcc tgccttctta
     3061 acaagtcctt cataggtgaa gtagagatgg agtccttcgg cgtcttgttc ttcgagcagt
     3121 gttttggtga gtcgcttgca gtaaatcatg agagccttgg ttgggcacat aacgtaaata
     3181 ttgggaaacg cctcgcctgt ggcatcctcc cactcgttac tagcatagaa ctcaaaatac
     3241 ttcttgatac gatttcgcat tcgatatctc ggtaaggtgg tgtcaaaaac cgacaggagg
     3301 aatcggatct ttttcttgga tttgcccgtg tatttcgtag cacagagttg gaagtcgaac
     3361 tcagtgagaa aatggaacgg cgactcggtg tcagctaagt tactttcggt cagtaggtcg
     3421 taggatagga tattgctggt tttggcttca agctcaagac agatatctgc aatcaagata
     3481 cagcgcgaaa tgaaagattc ttggcgactt ttctcacggt acaagcgttg gatgatttcg
     3541 gtacaacaat cgtcttgggt tttgagaaaa cgaataccgt tactgccaat ataataaatt
     3601 gccggcttgg tattctcgcc aaagttggtg gagtaaattc ttcccacata atctttttcg
     3661 tacaaatcac gaagccaatc gttgatccgt ttgtgatatt tatgcttaag aaaagtttgg
     3721 atttgaatgc gattaagaaa gcggtatttg tagagtaaga gtaggatggc ttgttgtttg
     3781 gtggttattt taggaagttt catggtagtg atgcgttaga ttgaattcgg tatgtgatga
     3841 atagttaagt tgtttagtaa agttatacgt tcagtagatt aaagaatgag aagtgagcag
     3901 agctgagata tagagaatac caaacccaac ctaagccata tatgtaaaga aaggtgtaac
     3961 acttttccac cttccaacct gaaaacaagc gattttctgt gtcagctctt tgatacaccc
     4021 gctgatacaa ttatgcaagc ccaactcttc ctattttagc ccattagccg tatactaagc
     4081 ccgtatgaaa aagaaaaaaa cacaacccag taccccgccc aataaagacg agttctttac
     4141 gcttttgaat aaggcaatcg acacacccga cccagcagtg agtcaaaaag ctgcgccgag
     4201 ttccggtggt tataccgata cacaaactcg tccaagtaaa actgaagata cttcggcgaa
     4261 acggcgtggt acgtcccgtc aatcgaacgt ttcacctgag accaaaaacc ctcaatagtg
     4321 ttggtgtgga ttttgccttt gacatactgt tcttttttat gattgatttg cttgtgagta
     4381 taaccccatt ttttagcatt atagtaactt gagtaagtgt cggtcatcag ctcactgccg
     4441 aacttgacgt gtttacgcat caatggcttg atggtgtaga cagccgtatc tacggttact
     4501 tcggctcgcg cttcgccacc acgttggacc acaccaacta cgggcatttt accaaccgcg
     4561 cctcggccgg taatcccttt cttgcggccg ccaacataca tttcatccat ctcaaccgta
     4621 ccctcgagtt gagtggtagg ttgtttcata agttcgcgga ttttataggc aatgcgccag
     4681 gcgcatttat atgtaacccc taagtgacgt tccagctctt tcgcggagac gccgttttta
     4741 gattgagagg caagatagag agcataaaac cagagcgtaa gctttgtcgg ggatttgtga
     4801 aaaatagagc cagctgtggg atggagttgg tggctacacc acgcacaagc aaagcacttt
     4861 cgcttagcaa cacggtagaa tccggtcttg ccgcacttcg ggcaaacagg gtcacttcca
     4921 tacttacggc gaaagatata cgtcaagcat gcattgtcat ctggaaagtc agcctgaaag
     4981 tgcttgattg tgtacttgtt catatatcta ccgtacatcc aggcatactt gccgtcaagg
     5041 gatagttgcc tttttatgca acatgacagg tacggccagt ctgttataat acctgcgtat
     5101 gtacaagacc cccatgcttc agcagtggca cgaaatgaaa gagcagtatc ccgactgtgt
     5161 gctgtttttt cgaatggggg atttttatga aatgtttggt gacgacgcta agattgggtc
     5221 ggaagtttta catattacgc taaccgcccg ggacaaaggc ggcgatggca agatgccaat
     5281 gtgcggagta cctttccatg cgagtgaaaa ctatgtcgcc cgtttagttc aggcaggcta
     5341 taaagttgct atttgcgaac aagtgtctca gccgggtaaa ggccttgtga aacgagaggt
     5401 cgtacgggtg gttacgtcgg gaaccttatt acgcgatgaa tcccttacgg acgcaggatc
     5461 acaattttta atcagtttag tgatcgatgc caaggcagtt ggtggggcgg cagctgattt
     5521 gtcgacagga cagttctttg cgtttgaatt ccgaggaaat cccgacgata ttgtccctct
     5581 cttaatgagt cattttcgtc cgcgggaatg tgtggtgccg cgcgtaacat atgaaaatag
     5641 taactatatt tcgctcattc gtcaggaacg ttcggctaat attacctgtg ctcacgattg
     5701 gttagtgtac attaaagatg ccgaaaagct gcttaagaat cattttcatg tacagagttt
     5761 ggatacgttt gggctttcag gcaagtcatt cgccataaag ggcgcagcag cactactggg
     5821 atatttacgc tacacgcaaa aagggaatgt cgagcatatt acgagcattc aggagtttca
     5881 tttatctggg cacctggtac tcgatcaggt aaccctcaaa caccttgaag taatacgaac
     5941 ggaccaagga gaagtggcag ggagtctctt tggtgccgtt aattatacgc agacggcggc
     6001 tgggcagcgg ctcttacagg actggttact ccatccggtc acggatatcc aggaaattac
     6061 cgctcgccaa gatgcggtaa ccgaattagt gagccggccg gagcttgttc agcagctccg
     6121 agatgcgctc gctccgatga gcgatagtga gcgtttggtg ggacggcttt cggttgggtt
     6181 aggatctcct cgcgatttgc gtcgcttggc tgatacgctc tggcatatcc atgagcaggt
     6241 acttcctctc ctctcgggag tgaaatcatc gcggttgcag caggttgcaa atcctcttgc
     6301 taaggagctt ttagagtgga cgatacgcgc ccgtgaaatt ctttttcccg atgccaagcc
     6361 gacactcgac gaaggatata ttttccagac ggggtggaac tcggagctgg atgtgcttcg
     6421 aggtttgacg agtggaaatc aagagtggct cactcagttt gaagaacagg agcggcaacg
     6481 gactggaatt cctaagttga aagttgtgta tagccgtgcc tttggttatg cgatagaaat
     6541 cacgaaactt cataaagata aagtccctcc tgattatgcg ccacgtcaaa ctttggtaaa
     6601 cgctgagcgt tacacaacgc aggccttagt aagctatgag cagaaggcct tatcggctat
     6661 ggatcaaatg attgtgttag aaaagcagtt gtttatgaat gaggttcact cggtggtggc
     6721 tgtctcaaaa ataatctccc acttagctta ctcgattgcc tcgctggatg tctttgtgtc
     6781 ttttgcctat agtgcccgga ctcaccgcta cattcgtccg gttataaccg aagaagctgg
     6841 ccttgctatc gaagggggcc gtcatcctgt gattgagcat attgttgaga cagactttat
     6901 cgcaaatgat acccggctcg ctcctgctca ttcgcttcag ctgattactg gcccgaatat
     6961 ggccggtaag tcaacctata ttcgccaagt agcattgatt gcgctccttg ctcatacggg
     7021 atcctttgtt ccggcgcaaa gtgcaacgat tggcgtgctg gatcgtattt ttacccggat
     7081 tggcgctcac gataatttgc gagcgggaca gtcaacattt atggtagaaa tgctcgaaac
     7141 ggcgcatatt ctccataatg ccacttctcg cagcttagtt attctggatg aagttggacg
     7201 gggtacgagc cctgaggatg gttttgccct ctcatgggcg attgctgaag ctttggcgca
     7261 tcagagtaaa ggtttagttc tctttgcaac tcattttcat gccttaaaag atatcgctca
     7321 acaccataca acggtgttca atagccactt ggcagtggct gtagataatg acgatctcat
     7381 ctttttacgc aaggtggtgg atggtcctac tgacgaaagc tacggcattg tcgtagcgga
     7441 aaaagcgggg gtgccaaaag cgattattaa acgggccaag cagctacgga cggatcagga
     7501 tagagtgggg aaacgcgtgg tgagtgtaga tcagttgtct ctcctggctg cgacaacgca
     7561 tgactacaag agtaaggtgg aagataagct atctcagata gacttaagtg ctacaacgcc
     7621 cttacaggca cttatcttgc tcaacgactt gcaagcatct ttaaaagaca cgtaagtgga
     7681 tgatcactct ctagagaaac gttgatgttt gctttcgaag ataccatcgg cggacaaaga
     7741 aagtaatggc aacaatagcg gcaaccgcca gacaggttat gagtgctaat cgattaatca
     7801 atgaactcgg tgtgtcttga atggcttgat tcgcgggaga aaacgtaggg gtgggggatg
     7861 ttgtttcagg actcggggta atgctcgtca gtaccgcgat tacaggtgtt ggagaaggag
     7921 tgctcttggg agttactttt ggtgatgatt tggatgtggc ctgggggatt gttgacaagg
     7981 tggtggcggt ggatgtgggt gtgggtggtt gctctaccac cagtgtatat gtttgggttg
     8041 ttgttccacc tagtccgcta gaagtaagtg tgtaagtcgt cgttttttca gggttgacgt
     8101 aggtagcgtc tgtattattg acgagtattc cgacgtcagg cgtaatggtt cgattcgtgc
     8161 tatatgaatc agtaaccacc aacatggctt tttcaccttt ttttattatt tctggttggg
     8221 ttttaaagac tctaataacg ggtgccggaa taggtaaaat agttaactct gttttgagct
     8281 tggccggggt atagttagta agggccgtgc ctcccaggga aaaaacgtaa tcagggagaa
     8341 gggttactcc cgtcgtgcct acttttttag aagcaaattt tagcccaaat gtttgagacc
     8401 gtgtccaaag ttgatcaggg tcttttgtta atgttatatc gataatgcca ttcgtttcct
     8461 gaatcgctag tttaaaaggt ttgtaggcga gaaacccact cacgtagtta ctatcgtacg
     8521 tcaaataatc tggatcatag gcgactttca tactgagtgt gtcaaactta ctactttcgc
     8581 cagtgacatc tatcgtaata tagggcgaat agacgtaact gacataaaag ggggagggag
     8641 tattcgtgac caggcccaga gtgttgggtg agagctctga agcgcgaggc gtcaaagagg
     8701 agaatatgaa tgcgaatatg cctataagag gaatgagcaa ggcgatgctt aataagataa
     8761 ttcgctgttt aatcatggga gaatcttttg aagagtaacg gcactcccct tagagggatt
     8821 ctcctgagcg cccatagttg ctaacgaaga tagagaaatc tttaatatcg actttattat
     8881 cgaaggtgaa gtcggctagc tcatgagcgc cagatatggc agtgccgtat ttcgagacaa
     8941 acatgctaaa gtcgcggatc gtaacagtat tatcgttatt aaggtctcct gcgcgtgatt
     9001 cactaaatga taacacctcg ggtgcatttg tgacggcgcg aattgtggtg gcaaggtatc
     9061 cgtttggttt tatccaaagg tccacagggg tagtgatcgt cgcaagggct tctttgattg
     9121 gtgcgtcact aaagtaacca aggagtgagt cttcaatgtc cgggctgttt gtagaagctg
     9181 ttgaaaaagt ggtaacagcc gcatcgctca ttttccctga ttcaagtgat tcaccagatt
     9241 ttttagctac gacttgagcc ttcatatccg ttgccacaaa gcctttggcg gctccttgta
     9301 gtggcactcg gaaacctgtg gtagtgctaa ggaatcgtgg cggtggatca aggttgaggg
     9361 tgactttttc gccgcttgct cgggtgaagg tgacggtacc ttgttgcgtt tgcgtcaggg
     9421 tgcgttgagc gctttcggtt gcccgcttat gaatacggcc cgtgaacttg gctgttttgg
     9481 ttactgctga ttgtgcctgt gctgagatag atgggatagt aacctctaat ttttcggtat
     9541 caaaaccact tggtagagga atgctcatga ctacattaga gagcacttct ggatattgat
     9601 tcgtataatt aaaagtagcg gtgagcgtgt catcgtaatt aaggatagtt ttatcgaggt
     9661 tgtagccgac ttcaagcatg ggaacggaat aagtaatccg tagggtactt agcgttgggg
     9721 taatcgtgga gtcgtccgaa tgggctttaa ctttaataca tagaaccgtt gaatcaggca
     9781 catcagcaat attctcggtc caaggcgaag cgcaagtatt gttttggtag gcactaaaac
     9841 taaaggtcat agaggtgcct ggaggtgtat ctttcgtgat ttcggtgctg acccatttag
     9901 tctgtatgtt cagagggctg gtaaatcgac tcgactgtgt ttcgggccac gtgtcggttt
     9961 cgtaagagcg tttgtttaga tcgaatctcc aaatctgatt atgattttta ataaagtaac
    10021 caattttact gacactatcg aaaaccactc cttcggctga aagggaagga tcttccttat
    10081 acattaatcg gttccaccaa ctcccgtttg atggggtgta gtcaagtatt tggctgagag
    10141 cattgccaaa gagataggct ttatttgctg attcgtccca tatagcgccg ctcgcaccgg
    10201 gtcccgaccc tgatttcacg cggtcgtagc ttgttttgtt tgcaatatcg agaatgagtg
    10261 gtcttgttat cccgttggcc cgatatccac caaaaagatg gatttcgttg gccgagggga
    10321 cggttaatgc ttttgcccct gacatcttgg tgaagaactt aaaaggtgcg acagtgattg
    10381 cgttagtgat cggtttatac tcaaggatgg tatcgatcac aaccccttgc ccgtttggct
    10441 gcatagtctc cccaccaata agatagaaag aatcagtgcc cgggatggat gctaccgttg
    10501 tttcttttag aacataaggt agtttcgttg tcgacgtggt cagtgtgtca gctacgggat
    10561 cgtatactaa gatttcatct gagagactaa ccgtcgcgcc cttgtcgttc aggttattcc
    10621 cgcccagtat gtatgctttg ttggttttac tatcccacag cacgctggtt attcttctta
    10681 gcgctggtag cgaggcgttt ttcttggtga ccactccttg actgggtgag taggtgtaaa
    10741 tgtcaggtaa aactttgcct tgatcttgca agaatccacc taagataatc aattgattat
    10801 tttgactgtc ccagacgcta ttgggaaagt taatgttgag tgggagttga gtgtttgtgt
    10861 tttcgagtct gccggggtaa gactgcaatt gaagtttgcc tgtcggaatg tctcgttcaa
    10921 gtgttacgtt ttgaccttgc tctaaaaggg tattgttaac agtgatagtt tttgtcgcgg
    10981 aatagatcgt atcagccgat ggtgtttgga aacgtaaaaa ataccaacct aggacaacca
    11041 caagggcaag cagaagtacg agcgccacaa aagtgtgttt tggttttgat atatggtgag
    11101 aaggagtgcc gtggtgactc atattaatac tcggactctc tttgcttcct gtgccagtaa
    11161 atatatccaa tgattcctat gagtataaga agaaggccga agataatagc catgagtttt
    11221 cctgcctgtg agccttggtt tgtagtcaca gatgaatcgt ttgaactcgt cgttgtcgca
    11281 ctcgccgagg gagtgagcgt ttgtacgtta gaagaaggag tggtggtcgt cgtcgtgaga
    11341 tttgttattg aacttgagga agtagtggct ttaggagatg ccttggttgt tgtttgaaca
    11401 gaggtgttcg ttttcgttgt tgtggtagcc gtagcagtcg tgcgtggggt tgtggttgct
    11461 ggagccgtcg ttgttggggt tggtgtgtta gttggcgtcg taggcgtaac tagtgaaaga
    11521 gtcgtacttc cgagtgaaaa gtctttcaca tttccttctc gcaaaacttc ggttcccgca
    11581 ttgtagctga ctgttgtcgt gcctgttgct ttgaccataa atttaaacgt aataacccga
    11641 gccgcgccag tcacgccgct cgcactgctg ccacgatctt ggctaatgag cccattcgtt
    11701 tcgtcagcag taatgctggt ggcggctaaa ggaaaggcag tcccagagtc atcaccttgg
    11761 gtatagtcta gcagcgcagg atcgtattta atcttgtagc caatcgtatc gatcgtggta
    11821 tcggcctgga tcatggcgat cactgaaatc tcttgtccga cctcggctga agtaggagcg
    11881 gaaaatatta aacttccatc tgctgcaaat gcaaaggggg tacacaagaa aacagtgcta
    11941 ataataaata atggtatcag ccatattcga caataatatg tgcgcatata tttatgatag
    12001 tcataaagcg aagtagtctg caagacaagg ggttctctat gtactgggca gtcttgtctg
    12061 agcgtgctat aacaacgaag taaagaaaag gagcgtgcat gggagtgatt catcagttgc
    12121 cagaagagat tgctattaag attgcggccg gagaagtggt tgagcggccg gcttctgtcg
    12181 tgaaggaact ggttgagaac tcactcgatg cgggtgcgac ttctattgaa gttagcttgg
    12241 atgatggtgg caagactttt atcgagatta aagataatgg ttccggcatg gatcgagaag
    12301 atgctgtttc ttgcttctta cgccatggta cttcgaagat aaaaga
//
LOCUS       DASWYB010000063        14784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_402564, whole genome shotgun sequence.
ACCESSION   DASWYB010000063 DASWYB010000000
VERSION     DASWYB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14784
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402564"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VGE59_03490"
     CDS             complement(<1..376)
                     /locus_tag="VGE59_03490"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY1074733.1"
                     /translation="MSEATRRVVRFGVQNYWRNGWLSLAATLMMAMTLFIVSVFALQA
                     YVIKITTKSIQDKLDMSIYVSDTPSEDAVAKLVSDLKATSEVKEVIYLDKSKVMSEWQ
                     KLQVDQSIKDQVNNDNNPLPRTI"
     gene            complement(384..1079)
                     /gene="ftsE"
                     /locus_tag="VGE59_03495"
     CDS             complement(384..1079)
                     /gene="ftsE"
                     /locus_tag="VGE59_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY1074734.1"
                     /translation="MIELQHVSKNYGTTPVLHDVNLAIEAGEFVSLVGQSGVGKSTLI
                     KLLTREEVPSSGRIYVAGREISELSRSELPIYRRKIGIIFQDFRLLNQKTVAENIAYA
                     LEVCGASSHDIKRRVPKILELVGLEGRQKNYPPELSGGEQQRVAIARALIHSPKVLIA
                     DEPTGNLDPVNTWEIIDLLYRINQLGTLIILATHNKQVVDRLHRRVIELRGGKIVRDE
                     ERGSYVLQKAKVV"
     gene            1415..3628
                     /locus_tag="VGE59_03500"
     CDS             1415..3628
                     /locus_tag="VGE59_03500"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEY1074735.1"
                     /translation="MALLLQGTNIATVLLTRPQSAYAATTVIQLSPTTGQDTWMNVSF
                     GQSHENDTTIQAQNWQCTSGHEWVYVQFPLTSIPDGASINSATLSFNIESRESLEDTG
                     IVSADDDTVQVSRVTTGWTEDVTFDSQPSIDSSSTYRTTTVTTASSGWLDLPVTKLVQ
                     DWKSETISNYGAMVEFKCDPDTIDKIGFTSSESASTSLRPKLTVVYNGGVTPTPTPSS
                     TVTPTPTSTPTSNPTPTPSIAPTSTATPTAQPTATPQVSVSCLGITITEATTVDTATI
                     TFTTKKSVTASLVWDTQSHTSPPGKENTKATLSAYPQENKKTDAKGTSHSFGLGGLSP
                     NTTYYYSLSFSDGTQDCENSLTTQPSPVVTTTSTPTSEATTTATTTPKPVKSGVPQAS
                     EIGSTSGSSARRSALSALTNGQAIATQTGTALIAGVTTVATLGLPAAQAITGLAQALG
                     SLPQTLTSAWMNLLGLFGIRKKRMPWGRVLSSTTGEPIVGAMVSILNQDKYGQVVERA
                     ITDKEGRYGFFVEPGHFSLWAQRNGYTFPSQVLPKSYRGATFPIGAERMIILDLFCDP
                     LEVTRPWVTTLKRIAMKAELLRLPLLVIGTALTISSLTVQVVPLTVILALLYVLFWIN
                     EFRHRRFGRHTIVVTDTHGQALAYTIVRLIDSKTNQIAFTKATDANGEIFVLVPAGTY
                     RLQLMVRGAAQAIEQTVTLPKGVPLKQMKIEVETQNEWAAQVPTSTVSPLSGNPV"
     gene            complement(3562..4896)
                     /locus_tag="VGE59_03505"
     CDS             complement(3562..4896)
                     /locus_tag="VGE59_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY1074736.1"
                     /translation="MIQTRTSPVLSFQPLWTPEVARIVSDFPKKGIFLTGIEGSDPKI
                     DGVRRNQLKEAHDFYQNYEARLEKIRELGITWLRFGMPYSQVHAARDHFDFSFCDKVV
                     KKCGELGITIMADLLHFGLPDWLHEELNEDQYFQNRFFPGEFARYAHAFAERYPHIRH
                     YTIVNEPYLTAFFSSKLGLWNEHRWGDGWQDDRPFVNATRNIAQAAILGRKAIEGVWD
                     HEGRKEELIFVQNESFEVSTAAPGSGREAEAERFNLRRFAVLDLIFGVRDETMRRYLL
                     DQGLSDAEYEWFMDYGSMTKTVLGIDHYPTCVHTFYKDRTQDNSPTDPYRLYEITKEY
                     WERYPLPLLHTEVNAWPDHALSLCQQTYDAMARLKQEGYPVIGMGWYGDDLQVGWHVA
                     MRGPRSKEENPVGLFYKGDAQPVALLFGQLLQTGFPDRGETVDVGTWAAHSF"
     gene            4998..5693
                     /locus_tag="VGE59_03510"
     CDS             4998..5693
                     /locus_tag="VGE59_03510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1074737.1"
                     /translation="MQQTWKDYFAYEYLDDHAGYFSKERTEQELDFLTKNIIPTKTTT
                     ILDLACGQGRHSIGLTQRGYSHITGVDRDEHLLSLAYQAAAKATVTIPFIQQDMRHLN
                     LDQTYDVIMLLFCTFGIESDEVNKDILKRLHAHLKPGGRIFFDMHNLFRFARRMKTQK
                     NDYAEFDLETLTLWDINADGNRELPLRLYTVPEFTERLAKVGLTITKIWGDYDASPYS
                     FDSERLLVLAQKA"
     gene            complement(5690..6271)
                     /locus_tag="VGE59_03515"
     CDS             complement(5690..6271)
                     /locus_tag="VGE59_03515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1074738.1"
                     /translation="METKQTIIHFVRHGEVENPQNVRYGRLPGFHISVEGRHQAEQSS
                     LFFLKRKISHIYSSPLERTQQTATLLGMSLPHVSITLDARILEVKTAGMFEGKSRDLH
                     FYYDTVPNPDAEMIEQVNARMYHFVEEKIIQHYGSEIMAVSHGDPLAILFHSLVYGEL
                     ESRWRIYPTYASIFSYVFEGLTLKSVWVTNQKG"
     gene            complement(6308..6691)
                     /locus_tag="VGE59_03520"
     CDS             complement(6308..6691)
                     /locus_tag="VGE59_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074739.1"
                     /translation="MVDLETIQKRIEQLDIAQQEINTIKTALDDALAEDDRYQQLDLQ
                     TREFAVQKKKIKDEIWAQPTYQDAMAKMKDIKEEMADLKEILNHELLEWRQTNNSDEI
                     IGADGTTRKLKVNVRLQQQRVKSNY"
     gene            complement(6790..7692)
                     /gene="murB"
                     /locus_tag="VGE59_03525"
     CDS             complement(6790..7692)
                     /gene="murB"
                     /locus_tag="VGE59_03525"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY1074740.1"
                     /translation="MLNLPHLQTNVPLGPFTTYQIGGLAEYFVGVTSSQELAAAVRVA
                     REANVPYFVLGCGANILIKDGGMKGLVIYNKANHVEFIPSRHQVWAESGAQIADLIEV
                     CRDKGLSGFEHFVGIPSTVGGAIWQNLHFLSPDRERTMYIEEFVLGGRILDENNKIQV
                     VDKKFFQFGYDDTILHHRKLIVLDVLFQLAPKSPSEIQAQMIANMNWRAEKQPQLWQW
                     PSCGSVFQKIEGVGAGRLIDEAGLKGHRIGNAQISEKHANYIVNLGGATARDVLDLIE
                     LVRAEVKAKTGYELTPEIGIIGEG"
     gene            7817..8626
                     /locus_tag="VGE59_03530"
     CDS             7817..8626
                     /locus_tag="VGE59_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1074741.1"
                     /translation="MLLTGKRILVMGLIDTRSIAWAIGQAAEAQGAEVVYTVQNERVS
                     NSLLKRSLRQTDRSLEDLRIICCDVTKGDELDILVGHQYGPFHGFVYSIAFASKADGL
                     LEGELSDAPRHPVNEGFEISAAGFSQVIHRLRWTNEHVQPTLLDNSSVIALTFDSQHT
                     YPNYNWMGVYKAALEATARYTARDLGPHGIRVNCISAGPLNTLSASGITGFDAIGDVW
                     PARAPLGWNLDEGKTLVANNAVCLLSDLMRGVTGEILHVDGGFHSVGIATQ"
     gene            complement(8767..10257)
                     /locus_tag="VGE59_03535"
     CDS             complement(8767..10257)
                     /locus_tag="VGE59_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074742.1"
                     /translation="MNLNAFPWARLASWAVVVAWLLLVVMPQPVKDWQADERQRAARH
                     TPQAETAARAKATEIVNRALANPHYSADAYQHDIRERLDSLGIEHRAIQEKLRAHVEK
                     CGGDALAVRHAYERWLVSPEGRAAYRKGGIYEPVYLRVNWEWWKVQYALFIIPMLLIF
                     SIRAFALRKTFAEGLALMWVSRWSLLGNAIVWPLGYFGYEPHLTLEAQVQQAARELRQ
                     FQGLPVPRVILPSVNVPGNLESLRVLSRIQSVWVVLRYIWGPPGQSWHRLLMIWRRPV
                     IRAVCVSIVASVVMLLSPSRASAAEVTEKEGGPTFLGLLWTSVYPTNPGAPPGQHVFW
                     LFKEGDVVVYNVQDPSLSVLEFAPRPLKVRCGSMAGTIGPFVNLHLDSSTPTRFGLEA
                     VLRNQLGRDTELKGPVYLYLTGEGHRAGFFTPNLRLLHQVEPGLKIGAATTLGYERGV
                     GLNCLFGPAVEIKTGKKSSLLLRFGEWLAGPLKGTQQFRTDFVLGF"
     gene            complement(10362..11420)
                     /locus_tag="VGE59_03540"
     CDS             complement(10362..11420)
                     /locus_tag="VGE59_03540"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY1074743.1"
                     /translation="MSEKKVTFLFGGPGSEREVSVATAKSALPSFDDQFRIQPVFITP
                     DRRWVVSENYVAPGEAWDVAMRLMTQSAVPSDIALETIETSSPDVVFIGLHGEYGEDG
                     TVQALLEARGLAYTGSNSEASALAMDKPKVLQILQDEDLLVPEFLEVTPEVTPSDVQA
                     FADFNGYPLVVLPADRGSSVGVTIVKQSEDLDSAIEQARTKSDRVMVTKYIAGQEVSC
                     GLLVTDKNELTALPVTDLNPIEGHDFFDYDAKYLAGECDEVTPSRLPEDVQTNVQEIA
                     RKVHQLLGADGYSRVDMIVTPNHEVYVLELNTLPGMTQTSIIPQQAKVHGLSFSELLA
                     TICNNIDRSNEDYLTFED"
     gene            complement(11434..11871)
                     /locus_tag="VGE59_03545"
     CDS             complement(11434..11871)
                     /locus_tag="VGE59_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074744.1"
                     /translation="MTEKKVTLRFPKVGAGVVIIQDGKTLLAKRKGSHSAGCWGSMGG
                     HVEFGETPVEAVKREAREELGIEIGNVQFVTCMNMIKEGKHYLDVSFTAEIVSGEPII
                     QEPDKIEEIGWYSLTELPDNLFDPVRVVLESLKTGENYFEIKQ"
     gene            complement(11868..12335)
                     /locus_tag="VGE59_03550"
     CDS             complement(11868..12335)
                     /locus_tag="VGE59_03550"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1074745.1"
                     /translation="MENKSKAADLKRGIDFIGVNCTFWCHDGKGKVLLHKRSQQCRDE
                     QGTWDSGAGSMEFGESFEQTVRREVLEEYGVEPLEITYIATENVIRHHNNRKTHWIKN
                     IHCVLVDPLQVKNNEPEKIDEIGWFDPDELPSPLHSQVALEVSLVKNFLKRNV"
     gene            complement(12325..>14784)
                     /gene="secA"
                     /locus_tag="VGE59_03555"
     CDS             complement(12325..>14784)
                     /gene="secA"
                     /locus_tag="VGE59_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY1074746.1"
                     /translation="QKPGDLLAKKTQALLDDYLPQVFAYAREASLRTLGMRPYDVQLI
                     GGIVLHQGKIAEMRTGEGKTLVAASPLILNALVGQGAHLVTVNDYLAKRDAGWNAPLY
                     HWLGLSVSYIGHDASYIYDPGVTIEGENDPRLQHFRRVSRPEAYAADITYGTNNEFGF
                     DYLRDHMVRSVADLRQRPFHFAIVDEVDSILIDEARTPLIISGPAAESTSLYATFSQI
                     VRTLKNEEDYTFDEKHRSVAITDNGIAKVEQALSVPHLYDNDNILLVHHLEEALRAQV
                     IYKKNKDYVVKDGEVIIVDEFTGRMMPGRRYSEGLHQAIEAKEGVEVKRESETLATIS
                     FQNLFRLYPKLSGMTGTAATEAEEFYKIYKLDVVVIPTHRPISRIDHQDLIYKTEEGK
                     LAAVIQDIKSKQANGQPVLVGTISVAKSEKLSKLLKKEGIEHQVLNAKQHEREAKVIT
                     NAGKPGAVTVATNMAGRGVDIILGGANPLLNDETKDDKDAFETWKQDQQRVLEAGGLH
                     VIGTERHESRRIDNQLRGRSGRQGDPGSSIFYVSTEDDLMRIFGGDRLRAIMQRMGLP
                     EDQPITHPMITRSIETAQKRVEGHNFDIRKHLVEYDDVMNKHREVVYRKRQKLLYLEK
                     PTQEGEDPFATPTSLHEELLELMSDEQITEYEAKVEKYGLALMQQVERQFYMQTIDQF
                     WIEHLNQMDQLRHGIGLQAYGQVDPLVAYKQRAYELFERLLAGIEMEVIHNLLKLEIV
                     ETPQQPQALPEQQMQFHGADESLAGGSIMATSENPEEAADQLQSAAEGSSPPSNEYKN
                     VGRNNPCPCGSGKKFKQCHGK"
BASE COUNT         3857 a         3913 c         3253 g         3761 t
ORIGIN      
        1 taatggttcg cggcaatggg ttgttgtcat tattcacctg atctttaatc gactgatcaa
       61 cctgcagttt ttgccattca ctcattactt ttgatttatc gagataaata acttctttca
      121 cttcgctcgt agccttaaga tcagaaacca gtttcgccac cgcatcttcg cttggtgtgt
      181 ccgagacata aatagacatg tccaacttat cttggattga tttggtcgtg attttaataa
      241 cataggcttg gagggcaaag acacttacaa taaagagcgt catggccatc attaatgtcg
      301 ctgccaacga aagccagccg ttgcgccaat aattttgaac cccgaatcga actacccgac
      361 gtgttgcttc agacatacaa tccttaaact actttcgctt tttggaggac ataacttcct
      421 cgttcttcgt cgcggacaat tttgccgccc cgcagttcta ttacgcgacg atgcaggcgg
      481 tcaaccacct gcttattgtg cgtggctaaa ataataagcg tccccagctg gttaatacgg
      541 tagagaagat caataatttc ccaggtatta accggatcca agttaccggt tggttcatcg
      601 gcaatcaaaa ctttaggaga atgaattaat gcccgggcaa ttgccacacg ctgctgctct
      661 cccccggaca gttctggggg gtaattcttt tgtcggcctt caaggcctac taactccaga
      721 atcttcggta ctcttcgttt aatatcgtga gagctcgcac cacagacttc aagggcgtaa
      781 gcaatattct cggcaactgt tttctgattt aataaacgga agtcctgaaa aataatgcca
      841 atcttacggc gataaattgg cagctccgaa cgagacagtt cagaaatttc tcggccagca
      901 acataaatac ggccactact tggcacttcc tcgcgagtaa ggagtttaat caaggttgat
      961 ttaccgaccc ccgactgacc gacgagcgac acaaactcgc ccgcctcaat tgcgagatta
     1021 acgtcatgga gaaccggggt tgtaccatag ttcttagaca cgtgttggag ttcaatcata
     1081 gacgagttct acgataacat acttcggcgg gattggaaaa cggacaagca aagaaggctt
     1141 cggatcttcg ccgatactcc gaacaacctc gaataaagcg atcaactctg ctacagtaga
     1201 gtcatgtatg ggcatgtttt aaaggaggat ttttatatct cggaaaagca accgtaacca
     1261 ccttgagcaa aggctcatcc atattcaacg atccacagga accttctttc ctgtttttaa
     1321 ccgttggcat aactggctga gaacgcacaa taaaacctac taccactggc acaacctgcg
     1381 acttgcccgc tccttccata ctttttttat cgtcgtggca ctcctgctcc aaggaaccaa
     1441 tattgccaca gtcctcctga cgcggcctca atcggcatac gcagcaacaa cagtcattca
     1501 acttagccca actaccggac aagacacttg gatgaatgtg agctttggcc aaagccacga
     1561 aaatgacacg acgatccagg cgcaaaactg gcaatgcacc agcggccacg agtgggtcta
     1621 tgtgcaattc ccccttacca gtatcccaga tggcgcctcg atcaatagcg caaccctctc
     1681 gttcaatatc gaatcgcgcg agtcattaga agacactggc attgtttcgg cagatgacga
     1741 cacagttcaa gtgagccgag tgacaactgg gtggacagag gatgttacct tcgacagtca
     1801 gccgagcatt gattcatcct caacctatcg gacaacaact gtcacaactg ccagttctgg
     1861 ctggctcgac ctacccgtaa cgaaactcgt tcaagattgg aaaagcgaaa caatttcaaa
     1921 ctatggcgcc atggtcgaat ttaaatgtga tccggataca atcgacaaaa taggttttac
     1981 ttcctctgaa tcagcctcga catctcttcg gccaaaactc acagttgtgt ataacggcgg
     2041 cgtcacgcct accccgactc cttcttcaac agtaacacca acccctacca gtacaccgac
     2101 aagtaatccc actccaactc cttctattgc tccaacctca acggccaccc ccacagccca
     2161 acccacagca acaccacagg tctcagtctc ctgcttaggt attactatca ccgaagcgac
     2221 aactgtcgac actgccacaa ttacgttcac cactaaaaag agtgtcaccg ctagcctcgt
     2281 ttgggatacc caaagtcata caagccctcc ggggaaagaa aacaccaagg caacactctc
     2341 ggcctatccg caagaaaata agaaaaccga tgcaaaaggg accagccact cttttggact
     2401 gggcggcctg agccctaata caacctacta ttacagtctc tccttttcag atggaaccca
     2461 ggactgtgaa aacagcctga cgacacaacc ttcccctgtc gtgacgacaa cctctacgcc
     2521 aaccagtgaa gcaactacaa ctgctaccac tacccctaaa ccagtaaaat ccggggtacc
     2581 acaggcatct gagattggat caacatcagg aagttcagct cgtcgctccg cgctcagcgc
     2641 tcttacaaac ggacaggcta ttgccacgca aaccgggacg gcattaatcg caggtgttac
     2701 caccgttgca acccttggcc tgcccgccgc ccaagcgata acaggattag cccaagcctt
     2761 ggggagcctc cctcaaacac tcacatcagc ctggatgaat ttactcgggc tgtttggcat
     2821 ccgcaagaaa cgtatgccgt ggggacgagt tttaagcagt acaaccggag agccgattgt
     2881 cggcgcgatg gtgagtattc ttaaccaaga taagtatggc caagtggtag agcgggccat
     2941 tacggacaag gaagggcgct atggcttttt tgtcgaacca ggtcatttct ccctctgggc
     3001 gcagaggaac ggttacacat ttccatcaca ggttcttccc aaatcttaca gaggagcaac
     3061 gtttcccatt ggcgccgaac gaatgattat tcttgacttg ttttgcgacc cgctcgaagt
     3121 cacacgtccc tgggttacta ctctgaagcg catcgcgatg aaagccgaac tgctacgcct
     3181 tcctctcctc gtcattggga cagccctcac gattagttcg ctgactgtcc aggttgttcc
     3241 tttaaccgtt atcctggctt tgctctatgt gctgttctgg attaacgaat tccgtcatcg
     3301 ccgatttgga cgacatacaa ttgtagtgac cgacactcat ggccaggccc ttgcttacac
     3361 gattgtccgc ctcattgata gcaagaccaa ccaaattgcc tttaccaaag cgaccgacgc
     3421 caacggagaa atttttgttc tcgttccggc cggaacatac cgtttacaac ttatggttcg
     3481 cggcgccgcc caagccatag aacaaacagt gacactcccc aaaggagtgc cactgaaaca
     3541 aatgaagatc gaagtagaaa ctcagaacga atgggcagcc caagtaccta catccacggt
     3601 ttcgcctcta tcgggaaacc ctgtttgaag taactgccca aagagcaacg cgacaggttg
     3661 cgcgtcgcct ttataaaaga gtcccactgg attttcttct ttactccgtg gacctcgcat
     3721 ggcaacatgc cagccaacct gaagatcatc gccataccaa cccatcccaa taacaggata
     3781 tccttcttgc ttaagccgag ccatcgcatc gtaggtttgc tgacagagtg aaagcgcatg
     3841 atctggccaa gcatttactt cggtgtgaag gaggggcaaa gggtaacgct cccaatactc
     3901 tttcgtaatc tcgtagagac gatacgggtc tgttggtgag ttatcttgag tgcggtcttt
     3961 atagaacgta tgaacacagg taggatagtg gtcaattccc aggaccgttt ttgtcatcga
     4021 gccatagtcc ataaaccatt cgtattctgc gtcactcaac ccttgatcga gaagataacg
     4081 ccgcatagtt tcatcccgga ctccaaaaat aagatctaag acggcaaacc ggcgtaaatt
     4141 gaaacgctca gcttcggcct ctcggcctga tccaggagca gcggtagaaa cttcaaaact
     4201 ttcattttga acaaagatga gttcttcttt gcggccttca tgatcccata ctccttcgat
     4261 tgccttacgg cctaatattg cagcctgggc aatgttacgg gtcgcattaa cgaacgggcg
     4321 gtcatcctgc cacccgtcgc cccaccgatg ttcgttccac agtcctaatt ttgaagagaa
     4381 aaatgctgtg aggtagggtt cgtttacaat tgtgtaatgt cggatatgtg ggtaacgctc
     4441 agcaaaagcg tgtgcatatc gggcaaactc acctggaaaa aaccgattct gaaagtactg
     4501 atcttcgtta agttcttcgt gaagccagtc aggtaatcca aaatggagca aatcggccat
     4561 aatagtaata ccgagctcgc cacacttttt aacaaccttg tcacagaacg aaaaatcaaa
     4621 atgatcacga gccgcatgca cttgtgaata cggcatacca aaacgcagcc aggtaatccc
     4681 cagttcacga attttttcta aacgggcttc ataattttga taaaaatcgt gcgcttcttt
     4741 gagctggttc cgacgaactc catcaatttt tgggtctgaa ccttctatac cagtgaggaa
     4801 aatgcctttt ttcggaaaat ccgaaacaat acgagcaact tcgggcgtcc aaagtggctg
     4861 aaacgagaga acgggggagg tgcgggtttg aatcatagcc aatagaatga cgtttcgttt
     4921 tccattcgtc aagcctgact tgtaagaaac atgaaatacc aattgttttc cttccctata
     4981 tttgttagcc tacccttatg caacaaactt ggaaagacta tttcgcttat gaatatctcg
     5041 acgaccatgc tggttacttt tcaaaagaac gaaccgagca agaactggat tttttaacta
     5101 aaaatattat acctaccaaa actacaacta ttttggattt agcctgcggc cagggccggc
     5161 atagtattgg actcacacaa cgaggctact cccacataac tggcgtggac agagacgagc
     5221 accttctttc tttagcgtat caagcagcag cgaaagctac tgttactatc ccgtttatac
     5281 aacaggacat gcgccatctt aatttagacc aaacatacga tgtgattatg cttctttttt
     5341 gcaccttcgg gatcgagtcc gatgaagtta acaaagatat cttaaaaagg cttcatgcac
     5401 atcttaaacc aggcggacga attttctttg atatgcacaa cctgttccgt ttcgcacggc
     5461 gtatgaaaac acagaagaac gactatgctg agttcgatct cgagactctc acactttggg
     5521 atataaacgc cgacggaaac agagaactcc ccctacggct ctacactgtg ccagaattta
     5581 ccgaacgact cgctaaagtc ggcctcacta tcacaaaaat atggggagac tatgacgcct
     5641 ccccatactc attcgattcg gaacgattat tagttttggc tcagaaagct taacctttct
     5701 gattcgtcac ccatacggac tttaacgtca ggccttcaaa cacataacta aaaattgaag
     5761 cgtaggtggg atatattcgc caacgtgatt caagctcgcc gtaaacgaga ctgtggaata
     5821 aaatggcaag cgggtcacca tgactaacgg ccattatttc gcttccgtag tgctgaataa
     5881 ttttttcttc aacaaaatga tacatccggg catttacttg ctcaatcatc tcagcatctg
     5941 gatttggaac tgtatcgtag taaaagtgga ggtctcgaga cttgccttcg aacatgccag
     6001 cggttttcac ctcaaggata cgggcatcaa gagtaatgga aacatgtggt agtgacatcc
     6061 caagcaatgt agcggtctgt tgtgtccgct ctaaagggct tgaatagatg tgggaaattt
     6121 tccgcttgag aaaaaagagg ctcgactgct cagcttgatg tctaccttca acgctaatat
     6181 ggaaacctgg caaacggcca taacgaacat tctgaggatt ctcaacttcc ccatgacgga
     6241 caaaatgaat aatcgtctgc tttgtttcca ttcccctcca cccggacgag tcgccccatt
     6301 cacacgacta gtagttcgat tttacacgtt gctgctgtag gcggacattt acttttaatt
     6361 ttcgcgtcgt accatcagca ccaatgatct cgtcactatt attagtttgg cgccattcta
     6421 aaagttcatg attcaaaatt tctttgaggt cagccatttc ctctttaata tccttcattt
     6481 ttgccatagc atcttgatat gttggctgtg cccagatctc gtctttaatc tttttctttt
     6541 gaactgcaaa ttcacgggtc tgtaagtcaa gctgctggta acgatcatcc tctgccagag
     6601 cgtcatcaag ggcggtttta atcgtgttaa tttcttgctg tgcgatatct aactgttcga
     6661 tccgtttctg aattgtctct aaatcaacca tacctttata atctcctttt tcttccttcg
     6721 caccatgcgg ctgtttttac gtcgcggaga ttggctccct agacgcaaaa atagacgtat
     6781 ggtgctcttc tacccttccc caataatacc tatttcaggg gtaagctcat agccggtctt
     6841 ggcctttacc tctgcccgaa caagctcaat taaatctagc acatccctcg cggttgcgcc
     6901 accaaggttt acaatatagt tcgcatgctt ctccgaaatt tgagcgtttc caatacgatg
     6961 cccttttaac cctgcctcat caatcaaccg tccagcacct accccctcga tcttttggaa
     7021 caccgaccca catgatggcc actgccacaa ctgcggttgc ttctcggctc tccagttcat
     7081 gttagcaatc atctgagcct gtatttcact cggtgacttc ggtgcgagct gaaaaagcac
     7141 gtctaagact atcagcttcc gatgatgcaa aatcgtatcg tcatatccaa attggaagaa
     7201 ctttttatcg acaacttgga ttttattatt ttcatctaga attctccccc caagcacaaa
     7261 ctcttcaatg tacattgttc gttctcggtc gggcgaaaga aagtgcaaat tctgccagat
     7321 cgccccgcct accgtacttg gaattcctac aaaatgttca aaccccgata aacctttgtc
     7381 gcgacagact tcgattaaat ccgcaatttg agcaccactc tcggcccaca cttggtggcg
     7441 agaaggaata aactctacat ggtttgcctt gttataaata acaagacctt tcattccacc
     7501 atctttaatt aaaatattcg caccgcatcc taaaacaaag tacggtacat tcgcctcacg
     7561 agccacccgc actgccgccg ccaattcctg cgaagaagtg actccaacaa aatattcagc
     7621 gaggccaccg atttggtagg tcgtgaacgg tccaagtggc acattggttt ggagatgagg
     7681 caaattcaac atacctttac cataacacac cgagcgcgaa gcttgcataa ccctagtaat
     7741 tttattaaaa tataccttgt tcgattcttg cagtttacca atccactttt aagagtggat
     7801 cagaggagtt tccaagatgc tactaaccgg gaaacggatc ctggtaatgg gtctgattga
     7861 tacgcgcagt attgcgtggg cgatcggtca ggcggcagaa gcccaagggg ctgaggtcgt
     7921 ctacacggtg caaaatgagc gagtaagcaa cagcttactg aaacgctcgc tccgtcagac
     7981 agataggtcg cttgaggatc tacgcatcat ctgctgtgac gttacaaagg gtgacgagct
     8041 agacatcctc gttggacatc aatacggccc tttccatggg ttcgtctact caatcgcatt
     8101 cgcttcaaaa gctgacggct tgctggaggg tgaactgtcc gatgcccctc ggcacccggt
     8161 caacgaaggt tttgagatca gcgctgcagg attctcgcaa gttattcacc gcctgaggtg
     8221 gacgaacgag catgtacaac caaccttgct cgacaattcc tcggtgatcg cgctaacctt
     8281 cgattcacag cacacctacc ccaactacaa ctggatgggt gtgtacaagg cggcgcttga
     8341 agccactgcc cggtacactg cccgagacct gggtccgcac ggtattcgcg tcaactgtat
     8401 ctcggctggc ccactcaaca ccctatctgc cagcggcatc accggattcg atgccattgg
     8461 agacgtctgg ccagcccgcg cgcccctggg atggaacctt gacgaaggca agacgctcgt
     8521 cgccaacaac gccgtgtgcc tgctcagcga cctcatgcgt ggagtaacgg gcgaaatact
     8581 ccacgtcgac ggcggattcc acagcgtggg aatcgccacg cagtagctcg caaccaccac
     8641 atctggccag gcgccagaat cacgagaggc ctggggagca atccccgggc tttttttata
     8701 taaaaaacag ggcgggggag cacatggctc ctccgccctg cgttgacaca aaactcaata
     8761 gaatcgtcag aagcccaaga caaagtctgt ccggaactgc tgagtcccct tgaggggtcc
     8821 agccagccac tcgccgaagc ggagtagcag gctggacttt ttgccggtct tgatctcgac
     8881 cgcgggtccg aataagcagt tcagacccac cccgcgttcg tagccgagcg tcgtggccgc
     8941 gccgatcttc aggcccggct ccacctgatg caagagccgc agattcggag tgaagaaccc
     9001 tgctcggtgc ccctcccccg tgaggtagag ataaaccggg cctttgagct cagtatctcg
     9061 accaagctga ttccgtagca cggcctcgag gccaaaccgc gtcggcgtgc ttgaatccag
     9121 gtgaaggttg acaaaggggc caattgtacc cgccatgctc ccacagcgta ctttgagtgg
     9181 gcggggggcg aattccagaa ccgacagaga tggatcttgg acgttgtaga cgaccacgtc
     9241 cccttccttg aacagccaga aaacatgctg cccaggtggt gcgccggggt tagtagggta
     9301 gaccgacgtc cacaagagac cgagaaacgt cgggccgccc tccttttccg taacttcggc
     9361 tgcggacgcc cgtgaaggcg agagcagcat gaccacactt gccacgatcg aaacacaaac
     9421 cgcgcggatc actggcctgc gccagatcat gagaagccga tgccacgact ggccgggcgg
     9481 tccccagata tagcggagga cgacccaaac cgactggatc cgcgaaagaa cacgcaagct
     9541 ttccaggttg cccggtacgt tcacgctcgg caggattacc cgtggaacgg gtaatccctg
     9601 gaattgccga agttctctgg ccgcttgctg cacctgcgct tccagagtca ggtgcggctc
     9661 gtagccaaag taaccaagcg gccacacgat tgcgttccct aacaaactcc accgcgagac
     9721 ccacatgagg gcgagccctt cagcaaaggt cttgcgcaac gcgaatgctc ggatcgagaa
     9781 gatcaacagc ataggaatga tgaagagcgc gtactgcacc ttccaccact cccagttcac
     9841 acgcagatac accggctcgt agataccacc cttacggtac gccgctcgtc cttccggcga
     9901 gacaagccac cgctcgtagg catgtctgac ggccagagca tcgccaccac acttttcgac
     9961 gtgtgccctc agcttttcct gaattgctcg gtgttcaata ccgagcgaat ccagtcgctc
    10021 acggatatcg tgctggtagg cgtcggcaga gtagtgagga ttggccagag cccgattcac
    10081 aatctcggta gctttggccc ttgccgctgt ctctgcctgc ggagtatgac gagccgcccg
    10141 ttggcgttcg tcagcttgcc agtctttgac tggctggggc ataacgacca gcaaaagcca
    10201 ggcaactacc accgcccagc tcgcgagtcg cgcccacgga aacgcgttaa ggttcatgtg
    10261 aggccctcct gcctcatgtc gtcgataaca aaacactcct gcaaaacgac agttgcatta
    10321 taggctagca acctaaaaga atcaatgctc tttagtaaag atcaatcttc aaacgtcaag
    10381 taatcttcgt ttgagcggtc aatattatta caaatggtag caagtagttc ggaaaaagag
    10441 aggccgtgga ctttagcttg ttgagggata atgcttgttt gcgtcatgcc aggcagggtg
    10501 ttgagctcaa gaacatacac ttcatgattt ggtgtcacaa tcatatcaac gcgactatag
    10561 ccatcagcac ccaaaagttg atgcactttg cgagcaattt cttgcacatt ggtttgaaca
    10621 tcttccggca agcgggaagg cgtgacttcg tcacattcac cggcaagata cttggcgtcg
    10681 tagtcgaaaa agtcatgacc ctcaatggga ttgaggtcgg taactggcag agccgtaagt
    10741 tcgtttttat cggttactaa aagcccacag ctgacttctt ggccggcgat atactttgta
    10801 accattaccc gatcggattt cgtacgggct tgctctattg ctgaatccaa gtcttcgctt
    10861 tgtttaacga tcgtaacgcc aaccgatgaa ccccgatccg ccggcaatac aaccagaggg
    10921 tagccgttaa agtctgcaaa agcttgaaca tcacttggtg taacttcggg cgttacttcg
    10981 agaaactcag gcacgagaag atcttcatct tggagaattt ggagcacttt gggtttatcc
    11041 atcgccaaag ccgatgcttc ggagttgctt cctgtgtagg caaggccgcg ggcttcaagt
    11101 aaggcctgga cggtgccgtc ctcaccgtac tccccatgga gaccaataaa caccacatcc
    11161 ggtgagcttg tttcgattgt ttcaagcgct atatccgatg gaacagccga ttgagtcatg
    11221 agcctcatcg ccacatccca cgcctcaccc ggagctacat agttttctga gacaacccat
    11281 cttcggtcag gagtaataaa aacgggctgg atgcggaact gatcatcaaa agaagggagt
    11341 gcacttttgg ctgtagccac tgatacttca cgttcgctgc ccgggccacc aaatagaaag
    11401 gttacttttt tctccgacat gctcttctcc attttattgt ttaatttcga aatagttctc
    11461 accggtttta agagactcca acacgactcg aacaggatca aaaaggttat ctggtagttc
    11521 agttaaagaa taccaaccga tttcttcaat tttatcgggc tcttgaatta ttggctcgcc
    11581 agaaactatt tcagcggtaa acgatacgtc aaggtagtgc ttaccctctt tgatcatatt
    11641 catacaggtt acgaactgaa cattaccaat ctcgattcct aactcttctc gagcttctcg
    11701 ttttactgct tctacaggag tttcgccaaa ctcaacatga ccgcccatag atccccagca
    11761 ccctgcacta tgggaccctt tacgtttggc taacaatgtt ttgccatctt ggataataac
    11821 aacccccgca ccgactttcg gaaatcgcaa agttactttt ttctctgtca tacatttctc
    11881 tttaaaaaat tttttactaa agagacttca agagcaacct gtgaatggag cggtgacggc
    11941 aactcatctg gatcaaacca gccaatctca tcaatctttt ctggttcgtt atttttgact
    12001 tgtagcgggt caacaagcac acaatgtata ttttttatcc aatgtgtttt tcgattatta
    12061 tgatgacgga tcacgttttc agttgctata tacgttattt ctaacggttc aactccatac
    12121 tcctctaaaa cctctctccg gacagtttgc tcaaatgatt cgccaaactc catcgagcct
    12181 gcaccagagt cccatgttcc ctgttcatcg cgacactgtt ggctcctctt atgcaaaagc
    12241 acttttccct taccatcgtg acaccaaaaa gtacagttaa caccaataaa atctatcccg
    12301 cgttttagat cggctgcttt tgatttattt tccatgacat tgtttgaatt tcttaccgct
    12361 cccacatgga caaggattat tgcgacccac atttttatac tcgtttgaag gtggagaaga
    12421 tccctcggcg gctgattgta gttggtcggc tgcttcttcg ggattttcgc ttgtagccat
    12481 tatgctaccg ccggcaagcg attcatccgc tccgtgaaat tgcatctgtt gttcaggcaa
    12541 ggcctgtggc tgttgaggag tttcaacaat ctcgagcttg agtaagttat gaataacctc
    12601 catctcaatg ccagcgagta aacgctcaaa aagttcatag gcgcgttgct tataggctac
    12661 aagaggatcc acctgaccat acgcctgcaa cccaatacca tgacggagct gatccatctg
    12721 gttaagatgt tctatccaga attgatcaat cgtttgcatg tagaactgcc gttcaacttg
    12781 ttgcattagc gccaaaccgt acttttcaac ttttgcttca tactcagtta tttgctcatc
    12841 gctcatgagt tcgagcaatt cttcgtgaag tgacgttggc gtagcaaatg ggtcttcgcc
    12901 ttcttgcgtt ggcttttcta aatacagcag tttctggcgc ttacgataga caacttcacg
    12961 atgtttattc atcacgtcat cgtattcaac taagtgcttg cgaatatcaa agttgtgacc
    13021 ttcgacccgt ttctgagccg tctcgattga acgggtgatc ataggatgcg taatcggctg
    13081 atcttcgggt aatcccattc tctgcataat ggctcgcaat cgatctccgc caaaaatgcg
    13141 cataaggtca tcttcggtag aaacatagaa aatagatgaa ccaggatcac cttgtcgacc
    13201 ggatcggcca cgaagctggt tatcgattcg gcgtgattca tgacgctcgg taccaataac
    13261 atgcagaccg ccggcttcga gcacacgctg ctgatcctgt ttccaggttt cgaatgcgtc
    13321 tttgtcgtct ttggtttcat cattcaagag aggatttgca ccgccaagaa taatgtcgac
    13381 accacgacca gccatgttgg ttgccacagt aacagcacct ggcttaccgg cattcgtaat
    13441 aaccttggcc tctctttcgt gctgcttggc attcaacact tggtgctcaa taccttcctt
    13501 cttcaagagc tttgagagct tttcggattt cgcgacagag atagttccca ctaacaccgg
    13561 ctgaccgttc gcttgcttcg atttaatatc ttgtataacg gccgctagct taccttcttc
    13621 cgtcttataa ataagatcct gatgatcaat acgggaaatc ggccgatgag taggaataac
    13681 gactacatca agtttataaa tcttataaaa ctcttcggct tctgttgccg ccgtcccggt
    13741 catgccagat agcttcggat agagacggaa taagttttgg aaggagatag tggccagtgt
    13801 ttcgctctct cgctttactt ccacgccttc tttggcttca atggcctgat gcagtccttc
    13861 gctataacgg cgtcccggca tcatacggcc tgtgaactca tcgacaataa taacttcgcc
    13921 gtcttttaca acgtagtctt tattcttctt gtaaataact tgagcccgaa gcgcttcttc
    13981 taagtgatgc acgagtaaaa tattatcgtt gtcgtataaa tgaggcacac ttagggcttg
    14041 ttcaacttta gcaataccat tatcggtaat cgcaaccgac cggtgctttt catcaaaggt
    14101 gtaatcttct tcgtttttga gagtgcgcac aatctgcgag aaagtagcat ataaactggt
    14161 actttctgca gcaggtccag aaataattaa gggtgtacgc gcttcatcaa ttaagatgct
    14221 gtcgacttcg tctacaatag caaagtgaaa tggacgctgg cgcaaatcgg caaccgaacg
    14281 caccatatgg tctcgcaggt aatcaaaccc aaactcgtta tttgtgccat aggtgatatc
    14341 tgccgcatag gcctcaggac gagaaacgcg gcgaaaatgt tgtaagcgag gatcgttttc
    14401 gccttcaatc gtaactccag gatcatatat ataagaagca tcgtgaccaa tgtacgaaac
    14461 agaaagaccg agccagtgat acaatggcgc attccaccct gcgtcacgtt tggccaagta
    14521 atcgttaact gtaaccaagt gagcgccctg ccctactaag gcgttcaaaa ttaatggcga
    14581 agccgccacc agagtttttc cttctcccgt cctcatttcg gcaattttcc cctggtgcaa
    14641 tacaatcccg ccaatcaact ggacatcata tggccgcata ccaagggtac gaagcgatgc
    14701 ttcacgagca taggcaaaca cctgcggaag gtagtcgtcg agcaaggctt gagtcttctt
    14761 agcgagaagg tcccccggtt tttg
//
LOCUS       DASWYB010000064         8144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_88223, whole genome shotgun sequence.
ACCESSION   DASWYB010000064 DASWYB010000000
VERSION     DASWYB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88223"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VGE59_03560"
     CDS             complement(<1..681)
                     /locus_tag="VGE59_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1074747.1"
                     /translation="MQKITTHLWFDKEAKEAAELYTSVFKDSRIKNSTTIHDTPSGDA
                     DIVTVELAGQEFTLLNGGPVFTFTPAISFLVACDNKDEVSELWKKLNDGGQALMELGE
                     YPFSEHYGWTQDRYGLSWQIMHTKEPIKQKITPTLMFVGDVCGKAEEAATFYTSVFHN
                     SKIGDIMRYEEGEGLDKPGTVKHMDFTLEGEQFGAMDSAYEHKFTFNEAISFMVHCET
                     QEEIDYYWD"
     gene            complement(885..1136)
                     /locus_tag="VGE59_03565"
     CDS             complement(885..1136)
                     /locus_tag="VGE59_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY1074748.1"
                     /translation="MKIRVKVKPGAKVEKIELVEQAELGLEDNLPTYIVSVKELPTEG
                     KANKAVVKALAHYFSVSQSDVQLTLGQTARFKLFEINKN"
     gene            complement(1133..1588)
                     /locus_tag="VGE59_03570"
     CDS             complement(1133..1588)
                     /locus_tag="VGE59_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEY1074749.1"
                     /translation="MKNFDITSLEGALAATQNNQLEEWIHAFLVDGSNPNAPLSEGLK
                     AIPSRVYSEPILFPLSKLTRVCGPEEGMEYTLEKEGWERKIATLVERVKSGWNPQPLL
                     SAGTGDEAFTIRDGSHRYETLVRAGIKKYWVIFFFDNDQDKNAFMKIHI"
     gene            complement(1591..2055)
                     /locus_tag="VGE59_03575"
     CDS             complement(1591..2055)
                     /locus_tag="VGE59_03575"
                     /inference="COORDINATES: protein motif:HMM:NF039500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HEY1074750.1"
                     /translation="MNINDLRRFLIYCGKEGYATISEEGMIIESDQSTSIRIKKGDWQ
                     YHDNYFGGEPYGGREVVFYQVKPVWMMTYYGCVGDSVEDLKSVYQHLKKALTLFPADA
                     PFRGPKEFTEGNYSYKNRWDGDVEKFSGEEEIYHHNQKIYTAKYIGGVVDVR"
     gene            complement(2353..2874)
                     /locus_tag="VGE59_03580"
     CDS             complement(2353..2874)
                     /locus_tag="VGE59_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074751.1"
                     /translation="MSLAERIARSVACTTMAALGWCENPELAATSILAADTGDGIVHI
                     VEFPNWEIPELISSVEVTLERGEDRRWARGELSAYGWWQDEHAEDYRPWRQQYFEVTW
                     SETKAPRIVYGTFTDTELPQEHPFTEAEIREIEFLAKVDRQRFDILDAHTRRCPTCAW
                     TLSKSHDDRTRNK"
     gene            3110..3967
                     /locus_tag="VGE59_03585"
     CDS             3110..3967
                     /locus_tag="VGE59_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074752.1"
                     /translation="MDNKTSQLELSEYRTRVEGALKQQFDDAYQSDQAEYGMALIKHA
                     NLSPFDPKDTLKNIEHLFTYIHESLLNKAIPPEESLRLFLLLYCHFFEWSEMYNLFWD
                     LGGIKLGNRAGSKVDEVRDSSSDAWGQLCTVAQGDMTPEKLQNITALMAKLNKGPVNI
                     NKKIRMITNRSPLIGGLLAELFDNTLRNGFSHNNYAIDNSGLTIHDEASGKLVKYTFG
                     ELNLLINKSKSFAGLLISLWRERLSELNERTLSGKECEYKVQYRETRFRINLMRGSLP
                     LGPEPISEE"
     gene            complement(3973..4644)
                     /locus_tag="VGE59_03590"
     CDS             complement(3973..4644)
                     /locus_tag="VGE59_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEY1074753.1"
                     /translation="MYSWWDLGESSGHLGNDLALYLIECPFCEVKGNFELAFHGEKTK
                     PNGRKVLNFDTYKCGNCAGFVQVTWSAGEYSHGPNRGMHAFIVQPWPKRVNQAPEHWP
                     LTVSRFWKQAHQSLKSETWDAAAIMSRSALQAIMKDKRAKGNNLEKQIDDLAEKGILP
                     QLIKDWAHELRLLGNKSAHPKSDEGDVDPQNVKDIIKFLDFMLKYTYDLPKEIDNYRD
                     RHLNA"
     gene            complement(4663..4824)
                     /locus_tag="VGE59_03595"
                     /pseudo
     CDS             complement(4663..4824)
                     /locus_tag="VGE59_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
     gene            complement(4867..5211)
                     /locus_tag="VGE59_03600"
                     /pseudo
     CDS             complement(4867..5211)
                     /locus_tag="VGE59_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            complement(5221..7062)
                     /locus_tag="VGE59_03605"
     CDS             complement(5221..7062)
                     /locus_tag="VGE59_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEY1074754.1"
                     /translation="MIVYAAPKQAFLEDVLSGQVEKKILTSFIRELGHATGKSEIDSW
                     RNSMQYMKGIIDDPEISDDAGVAIEYKIPQTSRRVDFILTGLNEANRNTAILIELKQW
                     SEALVTEKDAIVETFVGRNTREVAHPSYQAWTYACLLEDYNEAIETHDVHLVPCAYLH
                     NYEPDNNIRNDFYKDYLDKAPVFLRPDGRKLRDFIKQFVKYGDKNDILYQIDNGRIRP
                     SKNLADSLSALMQGKKEFALIDDQKLVYETALNLSDIASENNKQVLIVEGGPGTGKSV
                     VAINLLVELTNRRRVAQYVTRNSAPRLVYEAKLTGTLAKSRISNLFSGSGSFHALEPN
                     TFDALIVDEAHRLNARSGMFNHLGENQVKEIINASKLSIFFIDEDQRVTLQDIGEKSE
                     IHKWAETMGAHVTELALTSQFRCNGSDGYLAWLDHVLQIRETANTTLDGVDYDFRVCD
                     SPEEVRQLILEKNQNNKARMVAGYCWNWKSVNDKDAYDIEIGDFKAKWNLKSDGQAWI
                     IKPNSVSEVGCIHTCQGLELEYVGVIIGPDLVIRDGKIITDASKRASSDKSVQGYKKI
                     LAQNPEKAKTVADTIIKNTYRTLMTRGQKGCYVYCSDFETQEFLKNE"
     gene            complement(7059..7415)
                     /locus_tag="VGE59_03610"
     CDS             complement(7059..7415)
                     /locus_tag="VGE59_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEY1074755.1"
                     /translation="MDQLTKLQDQLEKFREERDWKQFHNPKDLAISLSLEASEVLEHF
                     QWKNVDEVKAHIIEKKEDIAAELADVLYWLLLLSKDLNVDLEAAFQKKMDKNAQKYPV
                     DKAKGTHKKYTELETE"
     gene            complement(7494..8123)
                     /locus_tag="VGE59_03615"
     CDS             complement(7494..8123)
                     /locus_tag="VGE59_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY1074756.1"
                     /translation="MAQIYAFGDSITYGSWDTEYSGWANRLRLYFDQKTQEEDTPFTY
                     FYNLGIPGENTDSLVKRFQSETEIRFRKNEDYTFLFAFGANDACFTPSKGEFQVPVER
                     YKQNLDSVINQAKEIASKVFILNITPVVDELTVAPIGKDKSRLNKYIEPYNQAVSELA
                     QKNSIQLIDVHSAFMQHDLPTLFCEDGLHPNAKGHEIIFDLMKRSLNIV"
BASE COUNT         2038 a         1822 c         1904 g         2380 t
ORIGIN      
        1 gtcccagtag taatcaatct cttcttgcgt ctcgcagtgc accataaacg aaatggcttc
       61 gttgaaagta aacttgtgtt catacgcact gtccatggcg ccaaactgct cgccttcgag
      121 cgtaaagtcc atgtgcttaa cagtgccggg cttgtctaag ccttcgcctt cctcgtagcg
      181 cataatgtcg ccgatttttg agttgtgaaa cacggacgta taaaaggtgg cagcttcttc
      241 ggctttcccg catacatcgc ccacaaacat aagggttggg gtgatcttct gtttaattgg
      301 ttctttggta tgcatgatct gccaggagag gccgtaccta tcctgcgtcc agccatagtg
      361 ctcactaaat gggtattcgc cgagctccat taacgcttgg ccgccgtcgt tgagtttttt
      421 ccagagctcg gatacctcgt ctttattatc gcaggcaacg agaaacgaaa tggcaggggt
      481 gaatgtaaag accggaccgc cgtttaagag cgtgaactct tggccggcta gctcgacggt
      541 cacgatatcg gcgtcacccg atggtgtatc gtgaatcgtc gtactatttt tgatacggga
      601 atctttgaac acagaggtgt aaagctccgc ggcttctttg gcttccttat cgaaccataa
      661 gtgtgtggtg attttttgca taaataaaag ctccttgact cttaaatagt accagaaaag
      721 attggtgagc ttaagagcag tttttggata gcagttagac gaagaatgca atcaggaatg
      781 caacaaggag gcacgcaagt aactgcaggc atgttctttg ctcgttacct cgccatggtc
      841 gaaagagggg atccccagtg gggttgaata tgtttggcat aatgctagtt tttatttatc
      901 tcaaagagct taaagcgtgc ggtttgcccc aatgttaact gcacatctga ctgggaaaca
      961 gaaaagtaat gcgctaacgc ttttacgacg gctttattcg ctttaccttc tgttggtaac
     1021 tctttgacac tgacaatata cgtaggtaga ttgtcctcta gcccgagttc cgcctgttca
     1081 accagctcga tcttttcaac cttcgctccc ggcttaacct taacccggat tttcatatgt
     1141 gaatcttcat aaaggcgttt ttatcttgat cattatcgaa aaagaaaatc acccaatatt
     1201 tttttatgcc agctcgcaca agtgtttcgt atcggtggct gccgtcgcgt attgtaaacg
     1261 cttcatcccc tgtaccagct gaaagtaagg gttgaggatt ccatccactc ttaactctct
     1321 caaccagcgt agcaatcttt ctctcccatc cttctttttc taacgtgtat tccatacctt
     1381 cttccggtcc acatacacgg gtaagtttag aaagtggaaa taagattggc tcagagtaaa
     1441 cccgagacgg gatggctttg agtccttcgg ataggggagc attggggttt gagccgtcaa
     1501 ctaaaaaagc atggatccac tcctccagtt gattgttttg cgtagcagca agagctcctt
     1561 cgagcgaagt aatatcaaaa tttttcatct ttacctcacg tccactacgc caccaatgta
     1621 cttggccgtg taaattttct ggttatggtg atagatttct tcctcgccgg aaaatttctc
     1681 aacatcacca tcccatctgt ttttgtaact ataattacct tcggtaaact ctttggggcc
     1741 gcgaaacggt gcatcagcgg gaaagagtgt taaagctttt ttcaggtgtt gataaacaga
     1801 ttttaaatcc tcaactgagt cgccaacgca gccgtagtat gtcatcatcc ataccggttt
     1861 tacttggtag aataccactt ctctgcctcc atacggttct ccaccaaaat aattatcatg
     1921 gtactgccaa tcgcctttct tgattcgaat tgaggtggac tgatcactct caatgatcat
     1981 gccttcttcg ctaatcgtgg cgtacccttc tttgccgcag tagataagaa accggcgtaa
     2041 gtcgtttata ttcatgtttg gattatactc cctttgtaaa ctccgtccac aattttgcgc
     2101 tccgtctcac catgcaacac tagtactagc ttgtgagtgt tagaagttaa tcttcacgac
     2161 attgtatccg gcaataaaca gaatccggat attagttttg cctatttgtt aaggaggacc
     2221 aggagttctc gaatatgaac cttttgtaca atttgtaagt gattttgtat gtgtactgaa
     2281 acctggggct atataaaaca gaagaccctc gcagcagcgg gaccgcgagg gtcttctgtg
     2341 cgagggcgaa agttacttgt tgcgcgtacg gtcgtcgtgg ctctttgaga gcgtccacgc
     2401 acacgttggg cagcgccgtg tgtgtgcgtc gagaatatcg aaacgttgac gatctacctt
     2461 tgccaggaac tcgatctccc gaatctcagc ttcggtaaac ggatgctcct ggggcagctc
     2521 tgtatccgtg aaagtgccat aaacgatccg cggagctttc gtttcgctcc acgttacttc
     2581 gaagtactgc tgccgccatg gacggtagtc ttcggcgtgc tcgtcttgcc accatccgta
     2641 ggcagagagt tctcctcgtg cccacctcct gtcttctccg cgttcaaggg tcacttcaac
     2701 gctgctgatg agttccggga tctcccagtt tgggaattcc acgatgtgca caattccatc
     2761 accggtatca gcggccagaa tggaagtagc agcgagctcc gggttctcgc accacccaag
     2821 cgctgccatt gtagtgcagg ccacggagcg agcaatacgt tctgcgagag acatgcttat
     2881 ccgtttaggt cccattgtgg gctctccatc tcatcaatgt gatgagcatt gtcggtccaa
     2941 ctgaccgaaa gtacaaaaaa tataacataa aatatatatc acgtcaatgg ttttatacca
     3001 aaaaatagca ggcttttaac ctgttatttc catggggggc caagtaggat tcgaacccac
     3061 gaccaattgc ttaagaggca acaagaggga aaaggagtat aatcggtgca tggacaacaa
     3121 aacatctcaa ttagaacttt ctgaatacag aacaagggtt gaaggcgcgt taaaacagca
     3181 atttgatgac gcgtaccaat cggatcaagc cgagtatggt atggcgctaa ttaaacacgc
     3241 gaacctaagt ccttttgacc ctaaagacac cttaaaaaat attgagcatt tatttacgta
     3301 tatccacgaa tcactactca ataaagcgat tcctccagag gagagccttc gactgttcct
     3361 attactatac tgtcacttct ttgagtggtc tgaaatgtat aatctgtttt gggaccttgg
     3421 cggtatcaaa ttgggtaatc gagcagggtc aaaggttgac gaagtcaggg atagttcgtc
     3481 agatgcatgg ggtcagctat gtacagtggc acagggtgat atgactccag aaaagttaca
     3541 aaacatcacg gcattaatgg cgaagttaaa caaaggtcca gtcaatataa ataaaaaaat
     3601 tcgaatgatt accaatcggt ccccgttaat cgggggtctt ctagcagagc tgtttgacaa
     3661 tactctcaga aatggttttt ctcataacaa ctatgcgatc gacaattcgg gtttaaccat
     3721 ccacgatgag gcgagcggta agttagtgaa atacacattt ggcgagctga atttgctaat
     3781 aaataagtct aaaagctttg caggtcttct aatttcactg tggcgtgagc gattaagtga
     3841 actcaatgaa agaactctgt caggaaaaga atgtgaatac aaggttcagt atcgtgaaac
     3901 gcggtttcgc attaacctga tgagaggaag tcttccactt gggcctgagc cgataagcga
     3961 agaatgaacg agttacgcgt ttagatgtcg gtctctatag ttgtcgatct ccttgggtaa
     4021 gtcgtatgtg tacttaagca tgaagtcaag aaacttgatt atgtccttga cgttctgagg
     4081 gtcaacatcg ccttcatcgg atttgggatg cgctgattta ttaccaagaa gtcttagttc
     4141 atgagcccaa tctttaatca actgaggaag aatccctttc tctgccagat catcaatctg
     4201 tttttcaaga ttgttccctt ttgccctttt gtctttcatt attgcctgaa gggcactcct
     4261 cgacattatt gcggcagcat cccaggtttc gcttttcaaa gactgatgtg cttgtttcca
     4321 aaatcgcgat acagtgagcg gccaatgctc gggggcttgg ttcacacgct ttggccacgg
     4381 ctgaacaatg aaagcatgca taccacgatt ggggccatga ctatattcac ctgctgacca
     4441 cgtaacttga acaaatcctg cacagttacc acacttatat gtatcaaagt taagaacttt
     4501 acggccgttg ggttttgttt tctcaccatg aaacgccaat tcgaagttac cctttacctc
     4561 gcaaaaagga cattcaatta agtatagagc taaatcattg ccaaggtgcc cagagctttc
     4621 tccaagatcc caccaactat acatgtctcc tcctaaatat acttataacc actgtgcaac
     4681 gccgctatgt cctgaacagg tcccacggcg gttttggctg aagctgtacg taccatcccg
     4741 gcagactgct gatgccccag cgggaacgga atcagagtaa atcggacgct gtacttggtt
     4801 accgtccaca tttgtatacg tgtccccaac tgcattgcca gagataggaa tccttgtccc
     4861 gtttgcgatg attttactaa tcgggtcaat cttatcttcc ttaaggagct tgcgagaaga
     4921 ctctataccg tcgatgtacg taacttcata cgttagtacc ttcttgccgt tcacaccctc
     4981 tgttgtgact ttcgtagtgc cggaagtaag cgatgaatcg cttacttctt tagtagaaaa
     5041 taggattgat tcttctctaa tctctgtttt agtttcaaca acaggtacac gtactgtcga
     5101 aatgggagat gtggtttgcg taggtgtgga agtatgtgac gaacttgcaa atgcaccagc
     5161 aacaaataaa ccagagatta cagtaattat ccaaacgcct aatttacgca ctactatttc
     5221 ctattcgttt tttagaaact cttgtgtctc aaaatcagag cagtacacat aacaaccttt
     5281 ttggccgcgg gtcatgaggg tgcggtaggt attcttaatg attgtgtcgg caacggtttt
     5341 cgctttttct gggttttgcg ccagaatctt tttatagcct tggacggact tgtcgctact
     5401 tgcgcgttta gaggcgtcgg taataatttt gccgtcacgg ataacaagat ccggaccaat
     5461 gataacgcca acgtactcaa gttccagccc ttggcaggtg tgaatgcagc caacttcgga
     5521 aacggaattc ggtttgataa tccaagcttg accgtcagat ttaaggttcc atttagcttt
     5581 aaaatcgccg atctcgatat cgtaggcatc tttgtcgttg acgctcttcc agttccagca
     5641 gtagccggca accatgcggg ctttgttgtt ctggtttttc tctaagataa gctggcgcac
     5701 ttcttccggt gagtcgcaga cgcgaaaatc gtagtctacg ccgtcgagag tggtattggc
     5761 ggtttctcga atttggagca catggtcgag ccacgctaag tagccgtcag aaccattgca
     5821 acgaaactgc gaggtaaggg caagttctgt cacgtgtgct cccatcgttt cggcccattt
     5881 gtgaatttcg gacttctcgc cgatatcctg aagagttacg cgttggtctt cgtcgataaa
     5941 gaagatgctt agttttgagg cgtttataat ctctttcact tggttttcgc ccaggtgatt
     6001 aaacattccc gaccgcgcat tgaggcggtg cgcttcgtcg acgatgagcg cgtcgaaggt
     6061 atttggctca agagcgtgga atgatccgga gccactaaag aggttagaga tacgtgattt
     6121 tgcgagcgtg ccggtcagct tagcttcgta gaccagtcgc ggtgcggagt tgcgcgtaac
     6181 atattgtgct acccggcgac ggtttgtaag ctcgacgagg agattaatcg cgacaactga
     6241 tttacctgtc cccgggccac cttcgacaat gagcacttgt ttgttgtttt cggaagcaat
     6301 atccgacaag ttaagtgctg tttcgtagac gagcttttgg tcgtcgatga gagcaaattc
     6361 ttttttgcct tgcatgaggg cgctcagtga atcagcgaga tttttggatg ggcggattcg
     6421 gccgttgtct atttgataga ggatgtcgtt tttgtcgccg tacttaacaa attgcttaat
     6481 aaaatcacgg agtttgcgac cgtcggggcg taagaaaacc ggggctttat cgaggtagtc
     6541 tttataaaag tcgttacgaa tattattgtc gggttcgtag ttgtgtaaat aggcgcatgg
     6601 tacaaggtgc acgtcgtgag tctcgatagc ttcgttgtag tcttcgagta gacacgcata
     6661 ggtccacgct tggtacgaag ggtgcgcgac ttcccgggtg ttcctgccta caaacgtttc
     6721 gacaatagca tctttttcgg ttactaaggc ttcgctccac tgtttgagtt caatgagaat
     6781 agcggtgtta cggttggcct catttagtcc ggtaaggata aaatcgacac ggcgagaagt
     6841 ctgtggaatt ttgtattcaa tggcaacgcc agcgtcgtca gatatttccg gatcgtcgat
     6901 aattcctttc atgtactgca tggagttacg ccaggagtcg atttcgcttt tacccgtggc
     6961 atggccaagc tcacgaatga acgacgttaa aattttcttc tcgacttgtc cggatagcac
     7021 gtcttcaaga aaggcttgtt tgggcgccgc atagacaatc actctgtttc aagctccgtg
     7081 tattttttat gcgtgccttt ggccttatcg actgggtatt tctgtgcgtt cttatccatc
     7141 ttcttttgaa aagcagcttc aagatctacg tttagatctt ttgagagcaa gagcagccaa
     7201 taaagcacat ctgccagctc tgctgcgata tcctctttct tctctataat atgagcttta
     7261 acctcgtcaa cgtttttcca ttgaaagtgt tccaaaactt cggaagcctc aagactgagt
     7321 gaaatagcta aatccttggg attatgaaac tgcttccagt ctcgctcttc ccgaaatttt
     7381 tctagctgat cttgtagttt tgtgagttga tccatttgcg cacagtatag ctctaaactc
     7441 agtattatgc tatgttcgtt aaccgacatt taacaaagtg accaactctg tatctagacg
     7501 atgttaagag accttttcat aaggtcaaaa ataatctcat ggcctttagc gtttggatgc
     7561 aggccatctt cgcagaagag ggtagggagg tcgtgttgca tgaatgcgga gtggacgtcg
     7621 atgagttgga tgctattctt ttgagcaagt tcactcacag cttggttgta tggctcgatg
     7681 tatttgttta agcgggattt gtctttgcct attggcgcga cggtgagttc gtctactacg
     7741 ggtgtaatat tgagaataaa aactttagaa gcaatttctt tagcttggtt gatgactgaa
     7801 tccaagttct gtttatagcg ttcaacggga acttggaact cgcctttgct tggagtaaag
     7861 caagcgtcgt tagcaccaaa ggcaaagaga aacgtatagt cttcattttt gcggaagcgg
     7921 atttcggttt ccgactgaaa gcgtttgacg aggctgtcgg tgttttctcc gggaatgccg
     7981 agattataaa agtaagtaaa tggcgtgtct tcttcttggg ttttttgatc aaaataaagc
     8041 cggagccggt ttgcccagcc ggagtattcg gtatcccacg atccatatgt gatgctgtcg
     8101 ccaaatgcgt atatttgtgc catggccaga gtatactcct tgag
//
LOCUS       DASWYB010000065        24364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_242746, whole genome shotgun sequence.
ACCESSION   DASWYB010000065 DASWYB010000000
VERSION     DASWYB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24364
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242746"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1442)
                     /gene="gatB"
                     /locus_tag="VGE59_03620"
     CDS             complement(30..1442)
                     /gene="gatB"
                     /locus_tag="VGE59_03620"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY1074757.1"
                     /translation="MTQYVPVIGMEIHAELTTRTKLFCRCSNTSSDLETPPNTAVCPV
                     CLGLPGALPVLNKRAVEATVQLGRSLGCTIPAVTKWDRKNYFYPDLPKSYQISQYDQP
                     LCSGGELVWFDRAGERHTVALTRIHLEEDTGKLMHPAGKNYSLIDYNRSSIPLLELVS
                     EPVITSAQQAKQFAEEYQHRLRELGIAEASMEKGQMRVEANISVIPAAWQDDPEKRLT
                     GVKIEIKNLNSFRAMERAVLYEIDRQSRVLNEGGTLRQETRGWNDAKGETYVMRTKET
                     ADDYRYFPDPDLRPLDLSWLEEEGTVTPFSARSQAFEQLLNHGVDPKHVHTITGDPDR
                     LAFFTRLALVEPEAIRLAVQWLNNEPRLTQFSPEEVRESLELLGQGSVSATLFKQALA
                     DAQPGGLKHSLERVTASNAIDDLAAVVAKVLQENEAEVARYRAGEEKLLGFFVGQLRQ
                     ALQGKGDPGALVSELKKQLS"
     gene            complement(1439..1924)
                     /locus_tag="VGE59_03625"
     CDS             complement(1439..1924)
                     /locus_tag="VGE59_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074758.1"
                     /translation="MAANSYKKKKQEDPIEVKLVLGLLRGMWWLITLPFRGLSSDSST
                     KKRTAGSLDTREVAARWSDIQTSIGLGGTTHFGSAIVSADKLLDHVLRQKGYAGETMG
                     DRLKSAKDDISPAVYHYAWQAHKLRNRLVHEVGGEVVSFEAKEAIKQYEAVLRELGAL
                     R"
     gene            complement(1926..2399)
                     /locus_tag="VGE59_03630"
     CDS             complement(1926..2399)
                     /locus_tag="VGE59_03630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014215924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1074759.1"
                     /translation="MVTISTTKGDITIELYESDAPKTTKNFKELAQKGYYDGVIFHRV
                     IDGFMIQGGDPNGTGTGGESIYGDTFEDELNPNADSYKTGYKEGVVAMANRGPNTNGS
                     QFFIMLKDTPLPKNYTIFGHVTSGMDVVHAIGKVAVDGNDKPLEEVKMTTVKVAQ"
     gene            complement(2512..3903)
                     /gene="gatA"
                     /locus_tag="VGE59_03635"
     CDS             complement(2512..3903)
                     /gene="gatA"
                     /locus_tag="VGE59_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY1074760.1"
                     /translation="MTLELIQQALASGQMTSVQVVSACFEVIEAKDTDINAFVRVYKD
                     EALVAAAASDARRAEGSVLGPLDGVPVAIKDNISYQDHGTSAGSAMLKEYVAPYDATV
                     VAKLKAGGAIIIGHVNMDEFAMGSTGESSAFGATKNPHDLSRVPGGSSSGAAAAVAAR
                     MVPVSYGSDTGGSIRQPAAFCGVVGLKPTYGAVSRYGLLAMASSLDQIGPIGATVADV
                     EVAYEAIKGFDRADSTSSVPETLPAKTSYRIGVPKQFYSDGLDVRIRDTLDATLSKLE
                     DEGHTIVRDLDIPLLDQSVAIYYLIVPAEVSSNLARYDGIRYGVHGEGVADSRTQGFG
                     DEVKRRIMLGTFVLSAGYSDAYYKRAQTARAALTEQLSGVFQEVDVLLSPVTPELPFK
                     LGEKTNDPLAMYLSDIYTLPVNLAGLPALSVPAAWVQEGNALLPIGLQIIGPQWSEPW
                     LCDLGKKIEVPHA"
     gene            complement(3900..4181)
                     /gene="gatC"
                     /locus_tag="VGE59_03640"
     CDS             complement(3900..4181)
                     /gene="gatC"
                     /locus_tag="VGE59_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY1074761.1"
                     /translation="MQVTLDMVRHMATLSRLELTDEEVARFADQLSAILSYASHLPVV
                     SGHTEPHTLRLDEDEVREEETEGASLLRNALALENGYVKVPAILDRSES"
     gene            complement(4239..5015)
                     /locus_tag="VGE59_03645"
     CDS             complement(4239..5015)
                     /locus_tag="VGE59_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074762.1"
                     /translation="MFEMLVKLGLSEKEAKVYLATLELGTDTAQNIALKADVNRATTY
                     VTLEKLMNQGLVTTSEEGKKTVFTASDPHELTHYLEEQKREIESKKQYLDGAISQLMA
                     VYNAKSGKPTVRFYQGGEGIAALNATSPEAGVFTDELLLIIPVNILELSRRPSVLSSL
                     FSGTPSLRYLCSSPSGEVSEATRQLLPANGLIVSDDILSFEGLLVIYPGWGVKWYAFE
                     THPYGMLLQSADFAQNMKSLFELAWAGSTAYSQQLMGSQT"
     gene            complement(5037..5927)
                     /gene="htpX"
                     /locus_tag="VGE59_03650"
     CDS             complement(5037..5927)
                     /gene="htpX"
                     /locus_tag="VGE59_03650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY1074763.1"
                     /translation="MYKQIEANKTKTVALIALFLVFITGLGWVLSYVYDNPLILWVAA
                     AVSIVQAWVSYYYSDKVALAVSGAQKLEGNERREVQRIVENLAITAGVPMPELYIIDD
                     TAPNAFATGRDPQHASIAVTRGLLDKLDRTELEGVLAHEMSHITNYDIRLTTVVVILV
                     GIIALASDWFMRSLWWGGHRRRDDDNQGNGSGYLMLIGIVLAILAPLAATLIQLAISR
                     KREFLADASGALLTRYPEGLASALEKIAADKEPLEVANKATAHLYFENPLKDYSGKIN
                     ALFSTHPPVEERIKALRSML"
     gene            complement(6009..6593)
                     /locus_tag="VGE59_03655"
     CDS             complement(6009..6593)
                     /locus_tag="VGE59_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY1074764.1"
                     /translation="MIYVIIVLVILALWAIGAYNSLVKHRVRAEEAWSDIDVQLKRRY
                     DLIPNLVETVKAYAKHEKELFTQVAEARSQAIHAKTPAARATADTMMTETLKTLFAVS
                     ESYPELKASDNFKELQRELSDTENKVEAARRFYNSNVRDLNIALDTFPTNIIGGMFHI
                     AKKDLFEAVEGERETPKVSFEDDASSKKSAQDES"
     gene            complement(6648..7490)
                     /locus_tag="VGE59_03660"
     CDS             complement(6648..7490)
                     /locus_tag="VGE59_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074765.1"
                     /translation="MDLPTTPNSGTPPPLPPLTPPPSRDGEVGSVPMPVPPPLPKDEP
                     KSDVTVPVAPPASPSVTPPITPTPVPSAPTHPSAPEPGKPVVPTMPAAPAVTPLVVPP
                     PGAIKPPVSPDAKEMGMPPMTPAPMSQPASPTETKSIPITVGSSPVSPPPMTPPSPMA
                     GPQAQAPTAPAPSVLPPSPGTIPTPLMTTPLSAPGAATPPKEHHGKAAIVILVLAILA
                     FIIGGVVIAASLCIGPTIPGLSSRFNCTDSTATSETTTDNDQGLVTSPAASTSPEADS
                     STGL"
     gene            complement(7637..8821)
                     /locus_tag="VGE59_03665"
     CDS             complement(7637..8821)
                     /locus_tag="VGE59_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY1074766.1"
                     /translation="MKQQITISKQGAALVVAFLLIFGSGYQLGQRKAVSTGVSAGVQN
                     QDKGKPEKVDFGLFWDAWKVVNDKYVKAPDDQERVYGAIAGMVAGLGDPFSVFMKPSD
                     STRFSEDISGNFEGIGAELVQKEGYITVVAPLDDSPAQKAGLLAGDIILKIDKTDVPA
                     SLEDAVAKIRGKKGTTVTLTVAREGKQRDISITRDQVEVKSVSYSLHNTTAVIKINQF
                     NANTTSLLDEALAKMKQDNATSLVVDLRNNPGGLLNVAVDITSRFIEPGVVVIERDRD
                     KKEEALSTTPVSSRISLPMVILANKGSASASEIFSGALQDAGRAKVVGETTFGKGSVQ
                     SVEQLKDGSAIRITIAEWLTPKKREINKQGIKPDVEVKMTEEDVAAKRDPQLDKAFEL
                     LK"
     gene            complement(8874..9950)
                     /gene="mnmA"
                     /locus_tag="VGE59_03670"
     CDS             complement(8874..9950)
                     /gene="mnmA"
                     /locus_tag="VGE59_03670"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY1074767.1"
                     /translation="MNMAEMKKVVVGMSGGVDSSLTAALLQEQGYFVIGVYMKNWSEP
                     IKGVEHCPWVQDQLDARRVADQLGIPFYTVNFEEIYKKQVIDAFFADYEAGRTPNPDV
                     LCNRFVKFDAFFKYAKSLGADFIATGHYAQVKNGEIFQGVDGKKDQSYFLWAIEKDVL
                     SQVLFPLGAMEKSEVRSEAEKRGLATAKKKDSQGICFIGQADVREFIKQHLKPREGNV
                     INAAGEVIGTHEGAWLYTLGQRAGISDIKWGDESQRPILYVTATDTRANTITVGEEPA
                     LYTSRLRAGSANWLGAIPASGTILTAKIRYGQPNTPCAMLKADLNGFYLTFEAPQRAI
                     TPGQSVVLYEGNRMVGGGIIEKSE"
     gene            complement(10260..10910)
                     /locus_tag="VGE59_03675"
     CDS             complement(10260..10910)
                     /locus_tag="VGE59_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY1074768.1"
                     /translation="MPIRTANNGVLALSMAAFAVLVSLTVLAEPVSAAPKTATVSKAV
                     SSSTTKGVRTKTAYQPIRRPAVSSKKVAVKKPKTTKKAYAASTTVSIPAEVPPSTDTS
                     GVVARAVSVIGSRYRWGGTSVSTGFDCSGLIKYALGERAGALPRTSAAMYSSVKKTGD
                     LQPGDLVFFGRGKVRHAALYVGNGQVVHASTPRSGVRVDSVHTLAKALGYMGSGRI"
     gene            complement(11213..12175)
                     /locus_tag="VGE59_03680"
     CDS             complement(11213..12175)
                     /locus_tag="VGE59_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY1074769.1"
                     /translation="MTWVDWIVIIFFIVGALIGFWRGFVAQVFLIVGLVVAIIFTFWS
                     FPIVGSWIEAHFQIPITFARPLSLLVIFFLSSSIFHFLAQFVHRLIAPMVQANMVNRG
                     LGSAVGAARHLLIASILLALLVTLPTSSKVKAQVDNSKTGRPLVRFALQLEKGLSRWI
                     QDEDLKSLSYRIIGTDQTTTTALNFSVSDPTVDETGEKELFVLTNASRVKEKQKPLLS
                     NDKLREVARAHAKDMLQKGYFSHLSPQGKDVAKRVGEANITVTLVGENLASAPTIEIA
                     HLGLLASPGHKKNIMSSDYNEVGIAVLDAGRYGKMVVEVFAKTY"
     gene            complement(12211..13362)
                     /locus_tag="VGE59_03685"
     CDS             complement(12211..13362)
                     /locus_tag="VGE59_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY1074770.1"
                     /translation="MDRIYLDYAASAPLSPEAVKVIETFLKGSPVGNPSSLHIEGRKM
                     QALLDRAHTTVAAILGVHPTEVVFTSGATESNNNLIRGVIRAWRLHHEGTPHLVMSAI
                     EHPSWRQVAKHEGADVTLIPVSTDGVVAASDVIKAIRANTVLVGCMYVNNEIGTVQPV
                     AEIGKGVEAYREKHNSRWPIFHTDAVQAFPYCNTHSGHLHADSFTLSGHKYGALGGIG
                     VLVLRKNMPFQSTMRGGGQEWGFRGGTENVLGAITLAAAMEHGDTHRGHLTQHAEGLQ
                     NLIESLIAQKLPKVQVLGQKVLRAPHISYLWLPGVVDEHIVHKLDLVGVSVSSGSACS
                     SGATLPSSTLVAMGYDEREAFGGLRLSFGRGTTRAEVTQAIGRLVEVLG"
     gene            13615..13827
                     /locus_tag="VGE59_03690"
     CDS             13615..13827
                     /locus_tag="VGE59_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074771.1"
                     /translation="MLTVMGLAFWGLTAGWMQITFFGGQRMVQGTASPQTFVQAGLLM
                     RYNIPAVAIVVALTVLRLLLYATLRV"
     gene            13996..14445
                     /locus_tag="VGE59_03695"
     CDS             13996..14445
                     /locus_tag="VGE59_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074772.1"
                     /translation="MKEPLDYTRAAAMMDAAFEERKRYISLGCDPDKMGYGLWRSSLP
                     SKGDGSIATSSQHSGDEMPLAQANRLGCGSIIAFVVSPFTGVAGWGIATLGVALWIAF
                     LVIANKSNPRAAFGSRTYSQYLDCTIVGAILTVLYVVILAATFFGRH"
     gene            14920..15735
                     /locus_tag="VGE59_03700"
     CDS             14920..15735
                     /locus_tag="VGE59_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074773.1"
                     /translation="MGYVYAPEAVRLRRVPKLENYGRLIKITRERLPGIGLIGGVIGG
                     SAIIGQQSIRSDLDLFALSTAEHIEDVLERVSDIVYQATQWHIGLRPSLLDFEVGRTT
                     LHHLDAGFYAHLQWAARNGGVIGEDPLPRIQSQHLSPREGACHYLTFKSRSLSIKRSS
                     FRGMPLHERLDALQTALESPIHVARKVLEAYGHPFEDTKACVQEVYRRHFPAHAEKLD
                     ALVTCDAWYTGQLIKHLRKFELGPYEEVLRALEPCLKTSFYFVRDNLLMLAHN"
     gene            complement(15784..16455)
                     /locus_tag="VGE59_03705"
     CDS             complement(15784..16455)
                     /locus_tag="VGE59_03705"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074774.1"
                     /translation="MDIATLTLNQRNMLALFDIKAQITVQYAYRKLGLPRPTTKQVLS
                     RLVELGYVERNGKQRGTYYSLRTEEKVLDEFGNDVVRVYKGKEAFLRFFRSLHSELKQ
                     GDFYWSFAFKSEYYDSEIGAFLLNYHTELTRKNVEDKTLVHEEVKIKVAQTYRNVPNL
                     QIKSTMQEIPTGMSITRNRIANLVWGDHPMIIEIKSPVIVERYQKFFESVWSKSFTVS
                     KPTHI"
     gene            16564..16995
                     /locus_tag="VGE59_03710"
     CDS             16564..16995
                     /locus_tag="VGE59_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074775.1"
                     /translation="MNINTLKWTRQPRIALEAIPAHYKVLRSIVCASPGQGPVYIMLC
                     FFHEGCDCCFQVRAHEVDSITLPFPGRDEVDTLTYMTVEIPGKTLFVEEFEDPKAEVN
                     CKQRFEDLCQTLPDEKSEDFMKTYIIRHGNEAWELRSQLWL"
     gene            17078..17374
                     /locus_tag="VGE59_03715"
     CDS             17078..17374
                     /locus_tag="VGE59_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074776.1"
                     /translation="MAALEGVSWEAALESIRKNITGEHNPGQPVRAQFSWHKNGLLEC
                     RLVISGMYVCHGKWINFEGKIYTHESHMTWAEGGFNLETGNGGWKELVNKPDHD"
     gene            complement(17414..18163)
                     /locus_tag="VGE59_03720"
     CDS             complement(17414..18163)
                     /locus_tag="VGE59_03720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY1074777.1"
                     /translation="MQFIDTHVHLTYPDFGDIGEVITRAKEVGVTRFVSPGLDFKSSE
                     QVIALAKSYPEIIPGIGLHPLGEAEELAPYADLAKLPEVKAIGEIGTDRNAGPMEDQE
                     KRLRFFLDLAVQTEKPALIHIRETWDETFQILADYPQLRGKAVIHCFTGTKKEASKMR
                     ELGLLMSVTAILARKNMSPETLEAVKEWPLDQMMLETDGPYLAWPGEKWPNEPSTVAK
                     VAQFIAELKGVSIEEVAERTTQTAERFFNLK"
     gene            complement(18187..19047)
                     /locus_tag="VGE59_03725"
     CDS             complement(18187..19047)
                     /locus_tag="VGE59_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY1074778.1"
                     /translation="MIAPFLAALAMGAQTVVDKFSLSKRKIPLSTFIPFLFLYLFFFS
                     ALMTPFLGRVNWVLLLSPQFLFLFLVLIILAITWNIFYYESLQKEKLYEFETILMLVP
                     LVTVVLSWVFFPETWDIRIGVAAIIAAAALVWSHWDKHHFSLNHYSLNLLVAVVLMAT
                     EDIIAAELLRDGVISPVSLYCVRTLILFGFFYAYYRPQAKTVDRGFLNAISLSGLLGA
                     IFMVLRYYGYKEVGIPFTALVTIAAPMGVYAASAMVLHERMRLKVIIAAAIISVAIIY
                     ATSILQFAGK"
     gene            complement(19069..20571)
                     /gene="metG"
                     /locus_tag="VGE59_03730"
     CDS             complement(19069..20571)
                     /gene="metG"
                     /locus_tag="VGE59_03730"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY1074779.1"
                     /translation="MSYITTPIYYVNGDPHIGSAYTTLVADVFARYSKYKGEDTFFLT
                     GTDEHGAKIAEAAAKAGKEPQAFTDEISAKFFAAWKELNIQPDGFIRTTNPKHKAYVQ
                     KFLQDLYDKGHIYKGEYKGWYCTGCEEFKTETQIGENNTCPIHLTPLNEVAEEAYLFK
                     LSAFETQIKEALNADTFKVSPSSRKNEVLGFIEHEGLRDVAISRKNVAWGVPLPWDES
                     HTVYVWVDALLNYLSAADVNGPEFKSGKRPEFPPTLQIIGKDILRFHALIWPAIILAA
                     DLPLPKELFVHGYFTSGGQKMSKSLGNVITPGELIGRYGVDATRWLLATAVPFGADGD
                     LTLDRLDERFNSDLANNFGNLFSRVASMILRYREGRVPDGIEVPLLHIEKTRDFLDAA
                     YESADLTKVTDTLRLHADNFNKYIEDEKPWALAKTDQKKLDEVLANLAENVFVLACFL
                     APFLPEATDKILAGFGTSVSQVDYATLGKANHTAGKTIPPLEPLFPRLAA"
     gene            complement(20669..21241)
                     /locus_tag="VGE59_03735"
     CDS             complement(20669..21241)
                     /locus_tag="VGE59_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074780.1"
                     /translation="MKRKFRDLLVRSEPLSEAEVAELGSALQELLRPHLNRLPMGRLS
                     DRSFLEGGSGGSHALRADNPVGLPMADVDGYYYLARNQEPREGVSLLNGWGLGRSGLF
                     HRFWIDLKVEPYSHPDTTCQWYERACAVTIVESSLLQLQSIGVTPVTVVDALMGNIRA
                     WSGSLQVRAAEAASLCHEVTELSDMLQRIA"
     gene            complement(21554..21949)
                     /locus_tag="VGE59_03740"
     CDS             complement(21554..21949)
                     /locus_tag="VGE59_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074781.1"
                     /translation="MAAMPGEVNVILIDCLGRSGAFGNRLDEVAERIGVPVEHICWRY
                     LYCDTRFISMPPGDLLREIQMRTRLPKKLFRLDAVVVALPPNPILSVYLVESLRVATG
                     RAPLVLDTFVRPDFVDLNGVLDFQELAVK"
     gene            complement(21977..22690)
                     /locus_tag="VGE59_03745"
     CDS             complement(21977..22690)
                     /locus_tag="VGE59_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase I"
                     /protein_id="HEY1074782.1"
                     /translation="MTEQALNSMTPQFFIIATPIGNRDDITTRAIQTLGEVDFIVAED
                     TRHTGQLMALYGIKKPFISFRDAPSVIMDRSIKEVLDRLSRGESGAYVTDAGTPGVSD
                     PGWRLVQALKSATIPLTALPGPAAVTMLISLADVPLEEYRFVGFLPKKKGFQTKVTDL
                     VGYLNHKEIRGVVFYESPNRIRATLTDFMSQSPKLQAIVGRELTKKFETVYYGPLTKD
                     FITALPEKGEYVVLLTRIY"
     gene            22967..23218
                     /locus_tag="VGE59_03750"
     CDS             22967..23218
                     /locus_tag="VGE59_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074783.1"
                     /translation="MTPAMIEEIAERARRAVEHSQTTVGRISRLRLEIERYRFLASIA
                     PAFRSSRVCFNPHEGEDFPDYDEKLTAAEQELASLTSGN"
     gene            complement(23270..24211)
                     /locus_tag="VGE59_03755"
     CDS             complement(23270..24211)
                     /locus_tag="VGE59_03755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEY1074784.1"
                     /translation="MILTPEQIKLRLKKLGVGADKYLGQHFLIDQSVLDTIYETGQTF
                     LCSCDTIVEVGPGLGVLTEELLNLAGGLVVAVEKDPVFATHLKPFLNVEEELQVVQGD
                     VLQVLDRNEHFPFVSEVKIDKGSVSIVNPLEIKDSALAPCEEHKANPEWVVIANIPYA
                     ITSPLLRKLVYRQNPPHDILVLVQKEAAERITAKPGDRNRGLLTVLIEVMAESTIVQN
                     VPPHSFWPAPEVDSSLLHLKLRPQAVVPIQERKQFEKVVIAGFSQKRKQLANSLAGGL
                     GIKTSQSRELLERAGIDPLRRAETLTLKEWQDLARSV"
BASE COUNT         6477 a         6301 c         5434 g         6152 t
ORIGIN      
        1 gaactcctta cttctgaaaa gctatgcatc tagcttagct gttttttgag ttcagataca
       61 agtgctcctg ggtcaccttt gccttggaga gcctgtcgaa gttgtccaac gaagaaccca
      121 agcaatttct cttctccagc acgatagcga gcaacttccg cttcgttttc ctgaagtacc
      181 ttagccacca cagcggctaa atcatcaatc gcgttagatg ccgtcacccg ttccagcgaa
      241 tgtttaagac cgcctggctg agcatcggcc aaggcctgct taaagagagt cgccgacacg
      301 cttccttgcc cgagtaattc aagtgactca cgaacctctt ccggagaaaa ttgcgttaac
      361 cgaggctcat tattaagcca ttgtacggct aaacgaatcg cttccggctc taccaatgcc
      421 aatcgggtga aaaacgcgag tcgatcaggg tcgccagtaa tcgtatgaac atgttttgga
      481 tcaaccccgt ggtttaatag ttgctcaaat gcctgagaac gagctgagaa tggcgttacc
      541 gtaccctctt cttctaacca agacaaatca agcgggcgaa ggtcgggatc cggaaagtaa
      601 cgataatcat cggctgtttc tttcgttcgc atcacatagg tctcaccctt ggcatcattc
      661 cagccccgtg tttcctgtcg gagggtacca ccttcgttta aaactcgact ttgacgatct
      721 atttcgtaga gcacggcacg ctccatggcg cggaaggagt taaggttttt aatttcaatc
      781 tttacaccag ttaaacgctt ctcgggatcg tcttgccaag cagccggaat tacggaaata
      841 ttggcttcga ctcgcatttg tcccttttcc atactcgctt cggcaatccc aagctcacgg
      901 aggcggtgct gatactcttc ggcaaattgc ttggcctgct gagccgaggt gataacgggt
      961 tcgctcacca gttctaaaag aggaatactc gaacgattat aatcaatcaa agaataattt
     1021 ttccccgccg ggtgcatgag cttccccgta tcttcttcga gatgaatgcg cgttaaggca
     1081 accgtatgac gctcccctgc gcgatcaaac cagaccaatt cccccccact gcaaagcggc
     1141 tgatcatact gactgatctg atagctcttt ggtaaatcag gataaaaata gttcttacga
     1201 tcccatttgg tgactgcagg gatcgtacat ccgagcgacc gtcccagctg aaccgttgct
     1261 tctaccgccc gtttattcaa gactggcaaa gcaccaggta accctaaaca gacggggcag
     1321 accgcagtat ttggcggagt ctccaaatca ctcgaagtat tagaacaccg acaaaacaac
     1381 ttcgtccgag ttgttagttc agcatgaatt tccattccaa taacagggac gtactgcgtc
     1441 atctgagagc tcccaattca cgcaaaacgg cttcgtactg cttaattgcc tcttttgctt
     1501 caaagctcac gacttcgccc cctacttcat gaaccaaacg gttacgtaac ttatgagcct
     1561 gccaggcata gtggtagacg gcaggagaaa tgtcgtcttt cgctgacttc aaccgatctc
     1621 ccattgtttc tcccgcatat cccttttggc gtaagacgtg atcaaggagc ttatcggccg
     1681 atacaatggc tgaaccaaag tgagtcgtac cacctaaccc aatactggtt tgaatgtcac
     1741 tccaccgcgc ggcgacctct cgggtatcga gggaaccagc tgttcgtttt ttcgtactag
     1801 aatcagaact caatccgcga aaaggcaagg taatgagcca ccacatgcca cgaagtagcc
     1861 cgagaacgag ctttacttcg atcgggtctt cctgcttctt ttttttgtaa gaattcgccg
     1921 ccatgttact gcgctacttt aaccgtcgtc atctttactt cttcaagagg cttgtcattc
     1981 ccatccacgg caacttttcc aatggcatgg acaacatcca taccagaagt aacatggcca
     2041 aaaatcgtgt agttctttgg caatggtgta tcttttaaca taatgaaaaa ttgggagccg
     2101 ttggtatttg ggccccgatt tgccatggca acaacccctt ctttgtaacc agttttatag
     2161 ctgtctgcat ttggattgag ctcatcttcg aacgtatctc cgtaaataga ttccccacct
     2221 gtaccggtcc cgtttgggtc accaccctga atcataaacc catctataac gcggtgaaaa
     2281 atcaccccat cgtaatagcc tttttgagct aattccttaa agttcttggt cgtctttggc
     2341 gcatccgact cgtaaagttc aattgtaata tcgcctttcg tggtagaaat agtaaccatg
     2401 gatgactcct tttttgttgg tgtttctgtt ttggttccga ttgcctgggc tccataaata
     2461 aacccaacaa caaggataac gagagcaata aattgtgaca caggtggaaa tttacgcatg
     2521 aggcacctct atttttttcc caaggtcgca gagccaaggc tcactccact gaggacctat
     2581 aatctgtaaa ccaataggta ataatgcgtt gccttcttgt acccaagccg ccggaacaga
     2641 taatgcaggc agccccgcta agttcactgg caaggtataa atatctgaaa ggtacatcgc
     2701 aagcggatca tttgtttttt caccgagttt aaacggtaat tcaggggtaa ccggactgag
     2761 gagaacatca acttcttgaa acactcctga caactgttca gttaacgccg ctcgtgcagt
     2821 ttgagcccgc ttatagtagg cgtcagaata cccagccgaa agaacaaatg tcccaagcat
     2881 aatacggcgc tttacttcat cgccaaagcc ttgggtacga gagtcggcca caccctctcc
     2941 gtgcacgcca tatcggattc cgtcgtaacg ggcaagattt gaactcactt cggctggtac
     3001 aattaaataa taaatcgcca ccgactgatc tagtaacgga atatcaaggt cacgaacaat
     3061 cgtatgacct tcgtcttcta acttggaaag agtagcgtca agcgtgtcac ggatacggac
     3121 atccaggcca tcactataaa actgcttcgg cacacctata cgataggatg tcttagccgg
     3181 aagcgtttct ggtaccgagg aggtactatc cgcccggtca aatcctttga tcgcttcata
     3241 tgcgacctcc acatcggcca ctgttgcgcc gattggacca atttggtcga gagaggatgc
     3301 catcgctaac aaaccatacc ggctcaccgc tccatacgtt ggttttaggc ccacgacgcc
     3361 gcaaaaagca gctggttgac ggatagagcc cccagtatca cttccgtaac tgacagggac
     3421 cattcttgct gcgacagccg cagccgcacc actggaggat cctcccggta cgcgtgacag
     3481 atcgtgaggg ttttttgtgg ctccaaaagc gctcgactct cccgtcgacc ccatggcaaa
     3541 ttcgtccata ttaacatggc caataataat tgccccgcca gcttttagtt ttgcaaccac
     3601 agtggcgtcg taaggcgcaa catactcttt gagcatggca ctccctgcac tcgtgccgtg
     3661 atcctggtac gaaatattat ctttaattgc caccggaacg ccatcaagag gcccaagcac
     3721 gcttccttct gcccgacgag catcactggc ggcagcggct acaagcgctt cgtctttata
     3781 aacccgaaca aaggcgttaa tatcggtatc cttggcttcg attacctcaa aacaagctga
     3841 tacaacttgg acgctcgtca tttggccgga agcaagggct tgttgtatta attcgagtgt
     3901 catgattcgg accgatctaa aatagcaggt actttgacgt acccattctc aagagctaag
     3961 gcattacgta aaagtgaagc tccttcggtt tcctcttctc ttacttcgtc ctcatctaaa
     4021 cgcaatgtat gtggctctgt atggccagag acaacgggta agtgagacgc gtaggagaga
     4081 atcgcggaga gctgatcggc aaaccgagcc acctcttcat cagtgagctc aagccgactg
     4141 agcgtagcca tatgacgaac catatctaaa gtaacttgca tggtgttatg gtaacggatt
     4201 accctgaaaa tggcaagttt aaaagtggcc ggagactcct acgtctgcga ccccatgagc
     4261 tgttgcgaat aagccgtact acctgcccag gcgagttcaa acaaggattt catattttga
     4321 gcaaagtcgg cactttgcag taacattcca tagggatgag tctcaaacgc ataccactta
     4381 acaccccaac caggataaat tacgagaagc ccctcaaatg acaatatatc atccgaaaca
     4441 atcagcccat tggcgggtaa aagctgccgc gtggcctcac tcacttcccc actcggcgag
     4501 gaacagagat aacggagaga gggagtcccc gaaaaaagtg acgataaaac agagggacga
     4561 cgagaaagct caagaatatt aacggggata attaagagaa gttcgtcagt gaacactcca
     4621 gcttctggcg aagtagcatt aagcgcggca atcccctctc ctccttgata aaaccgaacc
     4681 gttggtttcc cagatttggc attatacacc gccatcagct gagaaattgc tccatcaagg
     4741 tactgcttct ttgattcaat ttcgcgtttt tgttcttcca gataatgcgt taactcgtga
     4801 ggatcagacg cggtaaagac ggttttcttt ccctcttcag atgttgttac aagaccttgg
     4861 ttcatgagtt tttccaaggt cacatatgtc gtagcccggt tcacatcggc cttaagggca
     4921 atattctgag ccgtatctgt ccctaactca agtgtagcca agtacacctt ggcttctttt
     4981 tcggacaaac ctaattttac gagcatctcg aacatggcgc tcctctagtg attcccttat
     5041 aacatagacc gcaaggcttt aatacgttct tctacaggag gatgcgtgga aaagagggcg
     5101 tttatctttc ctgagtagtc tttaagtggg ttttcaaagt agaggtgagc ggtggcttta
     5161 ttggccactt cgaggggttc tttatccgcg gcaatctttt ctaaggcact ggcaagtcct
     5221 tcgggataac gggttaagag tgctccgctc gcatccgcta aaaattcccg cttgcgagaa
     5281 atggctaact gaatcaaggt tgctgcaaga ggtgccagaa ttgccagcac aataccaata
     5341 agcatgagat acccgctacc atttccttga ttatcatcat ctctgcgacg atgtcctccc
     5401 caccaaagcg aacgcatgaa ccagtctgaa gccaaagcaa taatgccaac caaaataacg
     5461 acaactgtgg tgagacggat atcgtaattc gtaatatgac tcatttcgtg cgctaaaacc
     5521 ccttcaagtt cagtacggtc gagtttatcg agtagacctc gggtaacggc tatagaggca
     5581 tgctggggat cacggcccgt tgcaaaggcg ttgggagccg tatcgtctat gatataaagc
     5641 tctggcatag ggacacccgc ggtaatagcc agattctcta caattctttg cacttccctc
     5701 ctctcgtttc cctcgagttt ttgggcaccc gaaactgcga gcgcaacttt gtccgagtaa
     5761 tagtaactta cccatgcctg aacaatactg actgcggcag caacccataa aataagcggg
     5821 ttgtcataga catatgagag aacccatcct aagcctgtaa tgaacaccaa aaacagggca
     5881 atgagagcca ctgtttttgt tttattcgct tcaatttgtt tgtacatacg agaattgccc
     5941 ttacttttaa ctgggtaatg tgccctcata ctagtgtacg aagacacatt cagtcaatga
     6001 gtagtgttct aactctcgtc ttgcgctgat ttctttgaag aagcatcatc ttcaaagctt
     6061 actttcggag tttcacgctc cccttcaacc gcttcaaaaa gatccttttt tgctatgtga
     6121 aacatgccac caattatatt tgtggggaat gtatcaagag cgatattgag atcccgtaca
     6181 ttggagttat agaatcggcg agctgcttcc accttatttt ccgtatcaga aagctcacgt
     6241 tgtaactctt taaagttatc gctggctttg agctcaggat aactttcact taccgcaaac
     6301 aaagttttaa gcgtttcggt catcattgtg tcggcggttg cccgagcagc gggcgttttc
     6361 gcatgtattg cttgcgaacg agcttccgca acttgcgtaa agagctcttt ctcatgcttg
     6421 gcgtatgctt ttactgtttc aactaggtta ggaatcagat cgtaacgacg tttaagctga
     6481 acatcaatat cgctccacgc ctcttcggca cgaacccgat gcttgactaa cgagttatag
     6541 gcaccgattg cccataaagc cagaataacg agaactataa taacgtaaat cataagtatt
     6601 ccttttgtag ctcactatgc gcctacaggg ttttccctgt caatatatta tagaccagtt
     6661 gagctatcgg cttctgggct tgtcgaagca gccggtgacg ttacaagacc ttgatcattg
     6721 tcggtagtcg tttcactggt agcggttgaa tctgtgcagt taaaccggga tgacaatcca
     6781 ggaatagtcg gccctataca gagtgaggcg gcaataacga caccaccaat aataaaggcg
     6841 agaatagcca gtactaagat gacaattgcc gctttaccgt ggtgttcttt cggtggcgtg
     6901 gctgctccag gagcagaaag cggggttgtc ataagtggtg ttgggatagt tcccggtgac
     6961 ggaggtaaga ctgaaggagc gggagctgtt ggtgcttgag cttgaggacc agccattgga
     7021 cttggcggag tcataggtgg tggactaacc gggctcgacc ccaccgtaat cggaatcgac
     7081 tttgtttcag taggagaggc aggttgtgac atcggagcgg gagtcatcgg gggcataccc
     7141 atctcttttg catcaggaga caccggtggc ttgattgccc ctggcggagg aacaacaagc
     7201 ggcgtcactg cgggagcagc tggcatggtc gggacgactg gcttgccagg ctctggcgcg
     7261 gaaggatgag taggcgcaga cggcacgggg gttggcgtta tgggtggagt aaccgaagga
     7321 ctcgcaggcg gcgcaacagg aactgttaca tcagactttg gttcgtcttt cggaagtggc
     7381 ggaggcacag gcattggaac agaacccact tccccatctc gagacggcgg cggtgtaaga
     7441 gggggtaacg gtgggggtgt tcctgagttt ggtgttgttg gtaaatccat gcaaagcctc
     7501 ctttacgcga gtgggattaa ctcactcgag aacttcgatt acagcaagaa gtctcacttc
     7561 aaaaactgaa tcgaaatagt catgtactca tccagcttag cgcactcaag agcgccaagc
     7621 aagcgacagt ccctgtttac ttcaagagct caaatgcttt gtcgagctgg ggatcacgtt
     7681 tggccgccac atcttcttca gtcattttaa cttcaacatc gggtttgatt ccctgtttat
     7741 taatttcccg tttctttgga gtgagccatt cagcgatagt aatccgaatc gctgacccat
     7801 ctttaagctg ctcaaccgat tggacagaac ctttgccaaa tgtggtctct cccacaactt
     7861 tggcccggcc tgcatcctgg agagccccag aaaagatttc gctcgcactg gccgaccctt
     7921 tattggccag aataaccatt ggtagactaa tccgagaaga aacgggggtc gtactgaggg
     7981 cttcttcctt cttgtctcgg tcgcgctcaa ttacgacgac ccctggctca ataaaccggc
     8041 tcgtaatatc tacggcaacg tttaagaggc ctcccgggtt attccgtaaa tcgacaacca
     8101 aggaagtggc attgtcttgt ttcatctttg ccaaggcttc atcgagaagg ctcgtcgtgt
     8161 tggcattaaa ctgattaatc ttgataacgg cggtcgtgtt atgaagcgaa tagctcacgc
     8221 tctttacttc aacctggtcc cgagtaatcg aaatatcccg ttgtttaccc tcacgagcca
     8281 ccgttaaggt cactgtcgtg cccttcttac cacgaatctt cgcgaccgca tcttcgagcg
     8341 aggccggaac gtcagtctta tctattttca aaataatatc gccagccaac aacccggctt
     8401 tctgcgccgg agaatcatca agtggagcga ccaccgtaat gtagccttcc ttctgaacaa
     8461 gctctgcccc aataccctca aagtttccgc tgatatcttc gctaaaacgg gttgaatctg
     8521 acggcttcat aaacacacta aacgggtcac ccaatcctgc taccattcca gcgatcgccc
     8581 catacacccg ttcttggtca tcaggagctt ttacatattt gtcgtttaca accttccaag
     8641 catcccaaaa gaggccaaag tcgacttttt cgggtttgcc cttgtcttga ttttgcactc
     8701 cggcactaac acctgtcgac acggcctttc tctgtccaag ttgatatcca ctcccaaaaa
     8761 taagtaaaaa agcaacaaca agcgctgcgc cttgtttgct aatcgtaatc tgttgtttca
     8821 cgcgcctcct ttatccgagc caagcccact gtgggttcct gacgaaattc tctctattca
     8881 ctcttctcga ttattcctcc accaaccata cggttcccct catacaagac aactgactga
     8941 ccaggtgtaa tcgcacgctg tggagcttca aaggtaagat aaaatccgtt caaatcagct
     9001 tttagcatag cacacggcgt attaggctga ccatatcgaa ttttagccgt aaggatagtc
     9061 ccagaagcag gaatagcacc caaccagttg gccgaaccgg ctcgtaaccg tgaggtgtat
     9121 aacgctggtt cttcaccaac cgtaattgtg ttggcccggg tatctgttgc cgtcacatac
     9181 agaattggcc gttgtgactc atctccccac ttgatatcac tgataccagc ccgctgccca
     9241 agcgtataga gccacgctcc ttcatgcgta ccaatcactt caccagccgc atttataaca
     9301 ttgccctctc tcggcttaag gtgctgttta ataaactccc gtacatctgc ctgaccaata
     9361 aaacaaatcc cttggctatc tttctttttg gccgtcgcaa ggccacgctt ctccgcctcg
     9421 ctccgcacct cagatttttc catcgctcct aacggaaaaa gaacctgaga gagaacatct
     9481 ttttcaatcg cccataagaa gtatgattgg tcttttttac catccacacc ttggaaaatc
     9541 tctccatttt tgacctgcgc ataatggccg gtggcaataa agtctgctcc aagggatttc
     9601 gcatacttaa aaaaagcatc gaattttaca aaacgattgc acaatacgtc tgggttagga
     9661 gtcctaccag cttcataatc agcaaaaaag gcgtcaataa cctgtttttt ataaatttct
     9721 tcaaagttaa ccgtatagaa aggaatcccc agttggtcgg caacacgtcg ggcgtcaagc
     9781 tgatcttgca cccagggaca atgctccacc cccttaattg gctccgacca gttcttcata
     9841 taaacaccaa tcacaaaata gccctgttct tgtaacagag ccgccgtaag cgaagagtct
     9901 acccccccgc tcatgccaac tacaactttt ttcatctcgg ccatattcat agccctgtaa
     9961 ttctactata gtttcaccgt tgagacaatt atgaagtata gattcaaccg cattaagtat
    10021 tatggattta gcgtgcgggg agtggggtcg ttaggggatg aggggccgct tcgcaactag
    10081 gccccttgtc ccctaactcc ggctgaaggc cagtaccaat tggctcagat tgccgcactg
    10141 cgctcgcaat gacatatcaa ctcagatcct gaaaggagtt caggatgaca catataaaaa
    10201 tctctccaaa ccaaaaaccg cctctttaca gaagcggttt ttggtttaat ttcgatcgtt
    10261 taaattcgac cggaccccat gtatccaaga gctttcgcaa gcgtgtgaac tgagtcaaca
    10321 cgaactccag atcttggcgt tgaggcatgg acgacctgac cattgcccac atacagtgcg
    10381 gcatggcgga cttttccgcg gccgaaaaag acaaggtcac caggctgtaa atcacccgtt
    10441 ttcttaaccg aggagtacat cgcggctgat gtgcgaggca atgcacctgc acgttcacct
    10501 aacgcgtact taattaaccc cgagcagtca aaaccggttg agacacttgt accaccccat
    10561 cgataccgtg atccaatcac actgaccgcc cgagcaacaa cacctgaggt gtcggtactg
    10621 ggaggaactt cagcgggaat agatacggtc gtggaagcag cgtaagcttt cttcgtcgtc
    10681 tttggcttct taaccgccac tttcttcgaa gaaacagctg gccgacgtat tggttggtac
    10741 gccgtctttg tccggacccc tttggtagta ctagaactta ctgcctttga gacagttgca
    10801 gtctttggtg cagcagaaac aggctcagct aacaccgtca gactgacgag aacagcaaac
    10861 gctgccatcg aaagcgcgag tacgccattg ttcgccgtac gaataggcac ctccagaatg
    10921 tcctattatc agcaactcac gtcgaccttt tagcgcctgc cggccttaag tctgtaaaac
    10981 agactctcgg cactccttta cgcccagagc gagaaagact aacccttccc taaaagtgtg
    11041 agttgtggcc ttgcggctgg cttaaagcgc agacggagct gtctctaagc tatttttaag
    11101 gaacacgaga acaaccttac cagatagggt ctatttgtca agccatacct atgcaaagag
    11161 caccagagaa gctggcgctc ttttggcatc tttcataatg aacttaggaa agctaatagg
    11221 tcttcgcaaa cacttcgact accatttttc catagcgccc cgcatccaat accgcaatcc
    11281 caacttcgtt gtagtcgcta ctcataatgt tcttcttatg gcccggggag gcaagtaagc
    11341 cgagatgagc aatctcaata gtcggcgcgc tggcaaggtt ttcaccgaca agcgtaaccg
    11401 ttatattggc ttctcctacc cgcttggcaa catcttttcc ctggggtgaa aggtgggaga
    11461 aatagccctt ttgtaacatg tcttttgcat gggcccgagc cacctcgcgc aatttatcgt
    11521 tcgaaagaag tggtttctgc ttctctttta cgcgtgaagc gttggtaagc acaaaaagtt
    11581 ctttttcgcc tgtttcatcg accgttggat cgcttactga aaaatttaaa gcagtcgtgg
    11641 tagtttgatc cgtcccgata atccggtaac tcaaagattt tagatcttcg tcttgaatcc
    11701 agcgtgaaag acccttttca agttgaaggg caaatcgtac cagtggccga cctgtctttg
    11761 aattatctac ttgggctttt actttcgacg aagttggcaa tgttaccaaa agagcaagca
    11821 aaatgctggc aatcaagaga tgacgagccg ctcctaccgc actgcccagt ccacggttta
    11881 ccatattggc ttgcaccatt ggtgcgatca gccgatgaac aaactgcgcc agaaaatgaa
    11941 aaatgctact cgaaagaaag aaaataacta agagactaag aggccgggca aatgtaatag
    12001 gaatttgaaa atgggcttca atccacgatc caacaattgg aaaactccaa aaggtaaaga
    12061 taatcgccac aactagcccc acaatcaaaa aaacttgggc aacaaatcct cgccagaaac
    12121 cgatcaaggc tcctacaata aaaaagataa tcacaatcca atcaacccac gtcatacacc
    12181 tatcctatca tgctcagcca aagaagaaag ctacccaaga acttcaacca aacgaccaat
    12241 cgcttgcgtt acttccgctc gagttgtccc gcggccgaaa cttaagcgca acccgccaaa
    12301 tgcctcgcgt tcatcgtacc ccatcgcaac caaggtcgat gaaggaagcg tggcaccact
    12361 cgagcatgcc gagccacttg ataccgaaac acccactaaa tcaagcttat gaacaatatg
    12421 ttcatcgaca acacctggaa gccagaggta agaaatgtga ggggcgcgga gtaccttttg
    12481 acctaagact tgtaccttag gtaatttttg agcaatgagc gactcgatta aattttgaag
    12541 accttccgca tgctgtgtta ggtgtccccg atgcgtatca ccgtgctcca tagcagctgc
    12601 cagagtaata gcgcctaaca cattttccgt cccacctcgg aaaccccatt cttgtccccc
    12661 tcctcgcatt gtgctctgaa atggcatgtt cttgcgcaaa accaacacac cgatccctcc
    12721 gagcgcaccg tacttatgac ccgagagagt aaaagaatcg gcatgcagat gaccactatg
    12781 cgtattgcaa tacggaaaag cctgaacggc gtcggtatga aaaatcggcc agcgagaatt
    12841 gtgtttttct cgatatgctt cgaccccttt gccaatctct gccaccggct gaaccgtacc
    12901 aatttcatta ttcacgtaca tacatccaac caatacagta ttagcgcgga tagccttgat
    12961 gacatccgat gctgcaacaa ctccatcagt ggaaactggg atgagcgtaa catcggctcc
    13021 ttcgtgcttt gccacctgac gccaagaagg atgctcgatt gccgacatca caaggtgtgg
    13081 cgtcccctca tgatgaagtc gccacgctcg aatcactcct cggataagat tgttattgct
    13141 ctctgttgcc ccagaggtaa aaactacttc cgtaggatgc acccctaaaa tagcggccac
    13201 cgtcgtatgt gcccgatcaa gtagggcctg catcttccgt ccctcgatat gcaaactcga
    13261 aggattccct acaggagagc cttttaaaaa cgtctcaatt actttgacag cttcgggaga
    13321 gagaggcgcc gaagcggcat aatctaaata tatccgatcc atacccctat cttaccagac
    13381 caaattactt ctcgtatacc attgacaaaa gtatagtcct atagtaacct tttaattgct
    13441 ttacagtaat tgccgcagtt tggcaccttt cagggtataa aaatgacaca ggatcacact
    13501 tcagaaggtc cggacgctta ctgcgtccga aacaatggca ttggcgggtt gctggcgctc
    13561 gcactcgcac tgctcgggct gatcttttcc ttgatcggcc atttggaggg cggcatgttg
    13621 accgtaatgg gtctcgcatt ctggggtcta accgcggggt ggatgcaaat caccttcttc
    13681 ggtggacagc gaatggttca gggaaccgcc agcccacaga cgttcgttca agccggactg
    13741 ctgatgcgct acaacatccc cgctgtggct atcgtcgtag ccctcaccgt gctccgactg
    13801 ctcctttacg cgactttacg cgtctgacct cgctccaccg gctcatcgac cgaaccgctc
    13861 ccgttgcacg cattgcaaca ggagcggttc tttagtttta gcgagcacga gacttactat
    13921 aagtacttga caaaaggtat atttttatat aagataattg cgtcaacggt ttctctacgc
    13981 tataaggatc agaagatgaa agaaccgctc gactatacga gagctgccgc aatgatggat
    14041 gcggcgttcg aagagcgaaa gaggtacatc tcacttggct gcgacccaga caagatgggt
    14101 tacggcctct ggcggagctc cctcccttcg aagggtgacg gctccattgc aacctcgtct
    14161 cagcacagcg gagatgagat gcccctggca caagcgaatc ggcttggctg cggtagcatc
    14221 atcgcgttcg tggtaagccc ttttaccgga gtcgccggct ggggtatcgc cactctcgga
    14281 gttgctctct ggatcgcctt tctggtgatc gcgaacaaaa gcaaccctcg cgccgccttc
    14341 ggaagccgca cgtacagcca gtacctggac tgcacgatcg tcggcgccat tctgactgta
    14401 ctgtatgtgg tcatcctggc agccacgttc ttcggccggc actagtccgg tatcctccat
    14461 ccacccctct atccgaccgc cccctgttgc acgtgttgca gcatggggcg gttctgcttt
    14521 tataaaaaag tacggagcaa cagccccgta caacgagttc caacgatttc attctcttac
    14581 gctcacggct tagcaataaa ggcagcgaga aaaccttctt gccgccaaat cgctagttct
    14641 cctgagctag ggatcgaaag ccaccggcca cgcggcagcg tatcaaagcc tcacccagta
    14701 atgcactttc tggcaacgtg accgtcaccc tgaagcacaa acagccagcc tcctcccttc
    14761 ggccaccata cccgacgccc ctcgggaatc caccgcaacg actgcatcaa tacgaacctg
    14821 gtctccattt ccccctcctc cttactctat tttatcatat aatatgctat actttcgact
    14881 aatcgcactg gtgcgattca atcagtcccg gaggggaaaa tgggatacgt atatgcacca
    14941 gaagcggtgc ggcttcgtcg cgtgccaaag ctagaaaact atgggcgtct catcaagatc
    15001 actcgagagc gactacctgg catcggcctt atcggtggag tcattggtgg ttcagccatc
    15061 attggccaac agtccatccg gagtgatctt gacctattcg cgctttctac cgccgaacac
    15121 atcgaagatg tcctcgaacg cgtcagcgac attgtttacc aagccactca atggcacatt
    15181 ggcctgagac ccagtttgct cgactttgag gtcggccgaa ccactctgca ccacctagat
    15241 gcagggttct atgcccatct ccaatgggca gcacgcaacg gaggcgttat cggggaagac
    15301 cctcttccga gaatccagag ccaacacctc agcccacgag aaggggcctg ccactacctc
    15361 acgttcaaga gtcgttcgct ctccataaag cgatcgtctt ttagaggtat gccgcttcac
    15421 gaacggctcg atgctctgca aaccgcgctc gaaagtccga tccacgtggc cagaaaagtc
    15481 cttgaggcgt atggtcaccc tttcgaagac accaaagcct gcgtccaaga agtctaccga
    15541 cgtcacttcc ctgctcatgc agagaaactc gatgcgcttg taacatgtga tgcctggtac
    15601 acagggcaac tcatcaaaca cttgcgtaaa ttcgagctag gaccgtacga agaagtgctc
    15661 cgcgcgctgg aaccatgcct caagacatcc ttttactttg tacgcgacaa cttgcttatg
    15721 ctcgcgcaca actaaccatc tccccgggcg ccagctcttc gctggttctc ggggagcttt
    15781 gttttagatg tgagttggct tgcttaccgt aaacgactta ctccacactg actcgaaaaa
    15841 cttctgatat cgctcaacga ttaccggact tttaatttca ataatcatgg gatggtcgcc
    15901 ccaaaccaaa ttagcgatac ggtttctcgt aatactcatc ccggtaggaa tttcttgcat
    15961 ggttgattta atctgtaaat tgggcacatt acgatatgtt tgagccactt ttattttcac
    16021 ttcttcatgc accaaagttt tatcttctac gttcttacgg gtcaattccg tatggtaatt
    16081 aagtaagaaa gcacctattt ctgagtcgta atattcgctt ttaaaagcaa atgaccaata
    16141 aaaatctccc tgtttaagct ctgagtggag agatcgaaaa aaacggagaa aggcttcttt
    16201 ccccttatag actcgtacca catcgttacc aaactcatct aaaacttttt cctcggttcg
    16261 aagactgtag tacgtgccac gctgtttgcc atttctctcc acgtatccaa gctcaaccaa
    16321 acgagataac acttgtttcg tagtaggacg aggcaaaccc aacttccgat aggcatattg
    16381 cacggtaatc tgagccttta tatcaaaaag cgccagcatg tttcgttgat ttaaggttaa
    16441 ggtagctata tccatttcac tcctgtttta ggaataaact atatacataa gtatacataa
    16501 atattctttt ctactcaata ccttgctaca gttggctcag tttttgcttc gtacgaggag
    16561 aacatgaaca tcaacacgtt aaagtggaca cgccagccta ggatcgcact agaggcaatt
    16621 ccggctcact acaaagttct tcgatcaatc gtgtgcgcga gcccaggaca aggtcctgtt
    16681 tacattatgc tttgcttctt ccatgaaggc tgtgattgtt gctttcaagt cagagcccat
    16741 gaggtagaca gcattacgct tccttttcct gggagagacg aggtagatac actcacctac
    16801 atgaccgttg agataccggg aaaaacgctc ttcgtcgaag agtttgagga tccaaaggca
    16861 gaagtgaact gtaagcaacg ctttgaagat ctttgccaaa cactaccgga tgagaagtcc
    16921 gaagacttca tgaaaacgta catcattcgc cacggtaatg aagcatggga gctaagaagc
    16981 caactttggc tctaagtttt gtccgagctt cggctcggac tctctcttca gcatttaatc
    17041 agaaatgctg aagtggagaa acgggaacat cataactatg gcagcactag aaggagtcag
    17101 ttgggaagca gcactcgagt caattcgaaa aaatataacc ggagaacata atcctggaca
    17161 acccgtcaga gctcaatttt cctggcataa gaatgggttg ctcgaatgcc gacttgtcat
    17221 tagtggaatg tatgtatgcc atggtaagtg gatcaatttc gagggaaaga tctacaccca
    17281 tgagagccac atgacttggg cggaaggggg cttcaactta gagacgggta acggcggctg
    17341 gaaagagctg gtaaacaagc ctgatcacga ttaacaactg cccgcacgac caagtaatag
    17401 gtacggcggg ctcttatttt aggttaaaaa aacgttcggc agtttgggtt gttctctctg
    17461 cgacttcttc aatagacacc ccttttaact ccgcgataaa ttgggctact ttcgccaccg
    17521 tactcggttc attcggccat ttttctcctg gccaggctaa ataagggcca tcggtttcga
    17581 gcatcatttg atctagtggc cattctttta ccgcttcgag cgtctctgga ctcatgtttt
    17641 ttcgcgccag aatggccgtt acagacatta aaagtcccaa ttcgcgcatc ttacttgctt
    17701 ctttttttgt gcccgtaaaa caatgaataa cggccttacc gcgtaattgc gggtagtcag
    17761 ctaaaatctg aaaggtttca tcccaggttt cgcgaatatg aattaaagct ggcttctcgg
    17821 tttggacagc taaatccaga aaaaaacgaa gacgtttttc ttgatcttcc atagggccag
    17881 catttctatc tgtcccgatc tctccgatcg ctttcacttc tggcaatttc gctaagtctg
    17941 catatggcgc taactcttca gcctctccta agggatgcaa accaatgcca ggaataattt
    18001 ctggatagct tttagctaac gcaattacct gctcggaaga tttaaaatcc aaacctggcg
    18061 acacaaaccg agtaacacct acctctttgg cacgagtaat aacttcgccg atatcgccaa
    18121 aatcggggta agttaaatga acatgtgtat cgataaactg catgaagtta gtatagcaga
    18181 gaaactttat ttccctgcaa attgcaggat cgatgtcgca taaataatag cgaccgaaat
    18241 aattgcagca gcaataatta ctttaagacg catacgttca tgaagcacca tcgccgatgc
    18301 ggcgtaaaca cccataggag cagcaatcgt cacgagtgca gtaaacggaa tgccaacttc
    18361 tttgtaacca tagtaacgaa gcaccataaa tatggctccc aaaagaccag acaaactaat
    18421 cgcattcaaa aatccacgat caactgtctt ggcttgcggg cggtaataag cgtagaagaa
    18481 accaaagaga ataagggtac ggacacagta aagagaaacc ggagaaataa ctccatcgcg
    18541 gagaagttcg gcagcaataa tatcttctgt cgccatcaaa acaaccgcga ccaaaaggtt
    18601 taggctgtag tgattcaaac taaagtgatg tttgtcccag tggctccaca ctaaagctgc
    18661 agccgcaata atagccgcga caccgatgcg gatatcccat gtctcaggaa agaaaaccca
    18721 cgacagtaca accgtcacta aaggcacgag cataagaata gtttcgaact cgtaaagctt
    18781 ttccttttgg agactttcgt agtaaaagat gttccatgtg atggcgagga taatcaggac
    18841 caaaaataag aaaaggaact gcggagaaag gagcaatacc caattaaccc gacctaaaaa
    18901 tggtgtcatg agagccgaaa aaaagaacag atacaaaaac aaaaacggaa taaacgtcga
    18961 gagcggaatt tttcgcttcg agagagagaa cttatcaaca accgtctgtg cgcccatggc
    19021 gagcgctgcc agaaatggtg caatcatatc cgcccctcct atgggccgtt acgccgctaa
    19081 ccgcgggaac aggggttcaa gtggtggaat tgtcttccct gccgtgtggt ttgctttacc
    19141 cagcgtagca tagtcaactt gacttacgct agtgccaaag ccggctagga ttttatctgt
    19201 tgcctcggga agaaatggag caaggaaaca agctaaaaca aatacatttt cagccaaatt
    19261 tgccagcacc tcatctagtt tcttctgatc cgtcttcgct aaagcccaag gcttctcgtc
    19321 ttcaatatac ttattgaaat tatccgcatg caggcgcaac gtgtcggtaa ccttggtcaa
    19381 atcagccgat tcgtaagccg cgtccaaaaa gtcgcgagtt ttctcgatat ggagcaaagg
    19441 tacttcaatt ccatcaggga ctcttccttc tctgtagcgg agaatcattg aagctactcg
    19501 gctaaacaaa ttaccaaaat tattggctaa gtcactatta aaacgctcat ccagtcgatc
    19561 caaggttaag tccccatcag cgccaaacgg gacagctgtc gcaagaagcc aacgagttgc
    19621 atcaactcca taacgaccaa ttagttcccc cggagtaatg acattgccta agctcttgga
    19681 cattttttgc cctccacttg taaagtaccc gtgaacaaag agttctttcg gcaagggtaa
    19741 atccgcggct aagatgatcg cgggccaaat taaggcatgg aatcgcaaaa tatctttacc
    19801 aataatctga agtgtgggcg gaaattcagg acgctttcca gacttaaatt ctggaccatt
    19861 aacatcagct gccgaaagat agtttaaaag agcgtctacc cacacataca ctgtatgtga
    19921 ctcgtcccag ggtaacggca cgccccacgc aacattttta cggctgatag cgacatcccg
    19981 aagaccttca tgctcaatga aacctaaaac ctcgtttttg cgagaggacg gcgacacttt
    20041 aaatgtatca gcgtttaatg cttcttttat ctgggtttcg aaagccgaaa gtttaaataa
    20101 gtaggcctct tcagcaacct cattcaacgg agttaagtgg atcggacacg tgttattttc
    20161 accaatttga gtttcagttt tgaactcttc acaacccgta cagtaccacc ccttatactc
    20221 ccctttatag atgtgtcctt tgtcatataa atcttgtaaa aatttctgta catacgcttt
    20281 atgcttcgga ttcgtcgtgc gaatgaatcc atctggctga atattgagtt ctttccaagc
    20341 agcgaaaaac ttagcagaga tttcatcagt aaaagcttgt ggctcttttc cagctttagc
    20401 agcagcctca gcaatttttg ccccgtgctc atcagttccc gttaagaaaa atgtatcttc
    20461 ccctttatat ttcgaataac gcgcaaaaac atcagccaca agcgtggtat acgcgctgcc
    20521 aatatgggga tcaccattca cataataaat aggagtcgta atgtagctca taaccctttt
    20581 attatacccg aagcaactaa aaacaaaaga aaagggcgcc tggcttattg ttataagcca
    20641 ggcgcctaag cggaaaggcg atcggactct acgcaatacg ctggagcata tccgagagct
    20701 cagtgacttc atgacacaaa ctggccgctt ctgcggctct tacctgaaga ctccccgacc
    20761 acgctcgtat attgcccatg agcgcatcca cgaccgtgac cggagttaca ccaatgctct
    20821 ggagctgaag gagcgaggac tcaacaatcg tgactgcgca ggcacgctca taccactggc
    20881 aagtggtatc agggtgtgaa tagggctcca ccttcagatc gatccaaaag cggtggaaca
    20941 accctgaccg gcctaatccc cagccattga gcagtgatac accttctcgg ggttcttgat
    21001 tccgggcaag gtagtaatag ccgtcaacgt cagccatcgg cagaccaacc ggattatcag
    21061 cacgaagcgc atggctacca cctgatcccc cctctagaaa actacgatcg gacagcctgc
    21121 ccattggtaa gcggttcaag tgaggcctga gcagttcttg aagagctgac ccgagttccg
    21181 caacttcagc ctcactcaaa ggctctgacc gtacgagtag atctcgaaac tttcttttca
    21241 caggatcccc cttttcgtca caataggttt tcaggttact ccttactcac cacccgacac
    21301 tataaattat actttaattc atataaatag caaaagcgct ggtggaccgt catgatctta
    21361 aaccagcgct ttatgcctta gcttaaggga ttttttgtgt tacagatgtg tcctaaattt
    21421 tggcgtggac acgaatgggg tggtagctgg ggttacttac agaggcctta aaggggaaga
    21481 gaccgaggct tagttggtct taattttatt tttcttaact taacttacgt aaattttttg
    21541 tttcggtttc tgattactta acagcgagtt cttggaagtc aagaacaccg ttgagatcca
    21601 caaagtcggg acgaacaaag gtatcaagaa caagaggcgc acgacctgtg gccacgcgta
    21661 acgactctac caggtacacc gacaagatcg gattgggcgg gagagccaca acgaccgcat
    21721 cgagccggaa gagcttcttg ggcaagcgtg ttcgcatctg aatctctcgg aggagatcac
    21781 ctggcggcat actaataaac ctagtatcac agtagaggta acgccagcag atgtgctcca
    21841 ccggaacacc aattcgctcc gcaacctcgt cgagacggtt cccgaaggca ccgctccgtc
    21901 ccaagcaatc aatcagaatc acgttcactt ctcctggcat ggctgccaaa agtggcagca
    21961 gacaaatatt acctaattag tatattctcg tcaagaggac cacgtactcc cctttttcgg
    22021 ggagagcagt aataaaatct ttggtgagag gtccgtaata cactgtttcg aattttttgg
    22081 tcagctcccg gccaacaata gcctgtaatt tagggctttg gctcataaaa tctgttaacg
    22141 tggcgcggat tcggtttggc gactcgtaaa atacgactcc ccggatttct ttgtgattca
    22201 aatagcctac taagtccgtg acttttgttt gaaagccttt tttcttaggt aaaaagccaa
    22261 caaaacgata ttcctctaat ggaacatcag ccagagagat aagcatcgtt acagccgcag
    22321 gtccaggcag ggcagttaaa ggaatcgtgg ccgatttcaa agcctgtact aaacgccagc
    22381 ccggatcact cactcctggc gtcccggcat cggtgacata tgcgcctgat tcgccgcgag
    22441 aaaggcgatc taagacttct tttatagatc ggtccataat tacgcttggc gcgtcacgaa
    22501 agctaataaa cggcttttta atgccataca atgccatgag ctgccccgta tggcgagtat
    22561 cttcagccac gataaaatca acctcaccca gtgtttgtat cgcgcgggtc gtaatatcgt
    22621 ctcggttccc gataggggtt gcaataataa aaaattgagg cgtcatagag ttaagagctt
    22681 gttcggtcac tatatcatga aatatgctaa actgtgagca tctcgaccgg agaactgcac
    22741 catgttaaaa ccgccgatct taccgcccct tactcccgac agggaagctc gcgttgcgcg
    22801 agtgagaact ctcgccaata gtccggaggg attggaacgg cttaagcgag cgtttgatgc
    22861 cgccaccgaa acctccagac agtttcgaga acgattgcag cctttgagac cggatggatc
    22921 ccccccttgg gttggcgacc tctggctcta aatgggaaga gcgaacatga caccagcgat
    22981 gatcgaagaa atcgccgaac gagctcgacg tgctgtcgag catagccaga caaccgtagg
    23041 cagaatcagc cgacttcgac tcgagattga gcgataccgt ttcctcgcga gcatcgctcc
    23101 agcgtttcga agtagccgag tatgcttcaa tcctcatgaa ggagaagact tccccgatta
    23161 cgacgaaaag ctaactgcgg cagaacaaga actggccagc ctgacctcag gcaattaagt
    23221 ctcgctcccc ctacccctga acagccccgg ccgtgtgtcg gggttttcct tatacgcttc
    23281 gtgcgaggtc ttgccactcc ttgagtgtga gcgtttccgc acgtcgcaat ggatcaatac
    23341 cggctctctc caacaactca cgggattgac tcgtttttat cccaaggcca ccagcgagac
    23401 tgttggcaag ttgctttcgc ttttgagaaa aacccgctat taccaccttc tcaaactgct
    23461 tgcgctcctg aatcggcaca acagcctgag gtcgaagctt gaggtgaagt aatgaactat
    23521 cgacttcggg agcaggccaa aatgaatgcg gtggcacgtt ttgcacaatc gtggattcag
    23581 ccatgacctc gataaggaca gttaataagc cgcgattacg atcccctggc ttggcggtaa
    23641 ttcgctcggc agcctctttt tgcactaaca ctaaaatatc gtgaggcgga ttttggcgat
    23701 acacgagctt tcttagtaaa ggtgacgtaa tcgcataagg gatattagca atcacaaccc
    23761 actcaggatt ggccttatgc tcttcgcaag gtgcgagcgc actatctttt atttcaagag
    23821 ggttaacaat agatacactc cctttatcta tcttaacctc cgagacaaag gggaagtgtt
    23881 cgtttcggtc taaaacttgt aatacatccc cttgaacaac ttggagctct tcctcaacgt
    23941 taagaaaagg ttttaaatga gttgcaaata caggatcttt ctcgactgca accacaaggc
    24001 cacccgcaag atttaataat tcctcggtta gaacacctaa accagggcca acttcgacga
    24061 tcgtgtcgca actgcacaaa aacgtttgcc ccgtttcata tatggtatcg agtacgcttt
    24121 gatcaattaa aaaatgctga cccagatact tatctgcgcc aacacccagc tttttgaggc
    24181 gtagtttaat ttgctcagga gttaaaatca ttaattctga ttatacggta tttttcatac
    24241 ttcgggcaga gcctaaggat tcaagatctc ttcgacttta atattttgca ttattccctg
    24301 tcccagactt ccgcctaatg ccttaaagtg gtccggatgt aaatcaagca cgcggttatt
    24361 acca
//
LOCUS       DASWYB010000066        23736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_35044, whole genome shotgun sequence.
ACCESSION   DASWYB010000066 DASWYB010000000
VERSION     DASWYB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23736
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35044"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="VGE59_03760"
     CDS             <1..1921
                     /locus_tag="VGE59_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY1074785.1"
                     /translation="LMLQHGVTMVDTPTNVAEFNISETGSRPPGTQALDYFTSFGKVG
                     NANYTWQRNFGTSLDALKAGKTFMAVGYLNDMAGLGALGQTNIGVAALPQVDVSNPQT
                     YGRYLAATVTKQAAQSSKGRDEKKLQAAWDFVALFANPYVSDSYAKSLRVVPARKDVA
                     KKQTFGEYYDPFISQVPYAVTWNKDEVAVADGALKEALTLAIDKKQDSQVALDVAAKG
                     YTSFLQRDRGINTDEKVMNFWMSEDDTTVYKETIRNYLGENKDIKRIATSKRSPARFE
                     WEILNAMAARKGPDLLALPSDAVVRYSPLLRGLPLKGFNKSTRISDTEALNLSYSPAV
                     KTDNYINGKLYGMPAQIETLMIGYNSNLQNQVRRAWQDKNFETDPTDDPFSSELILWK
                     DLKRRAEITSQVMQDVRYIALGTGSNVEHSADIFAAILKQYGGEMTDPDRNVTGIHLP
                     ISSRQTVVPGQEAEKLIRSYADTSSEWNSSLPNSLQALAEGKVVAAFIYPRDIAKIKA
                     LNPELSVSYIPLPQLTDTGDPIDFSSSYSLTVPLASKKAAKAYNFIVTSVFDGGQNDN
                     LIPPTLGRLKTPILERRRGGSVQTMQRNAAQSYYKGGHPKEIDQAIIDFLDRKRTLDQ
                     TANVINQLLKKNVAD"
     gene            1951..2358
                     /locus_tag="VGE59_03765"
     CDS             1951..2358
                     /locus_tag="VGE59_03765"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074786.1"
                     /translation="MSFLSLIVGVQTASAQFLFKRPTATPPPIKLPNFLPNISIDPET
                     IAQSILNLVVTWITLIAGIAAFVYILYGGWKYITSAGNPSEAQVAKKMIIGAIIGLLI
                     IALAYIVLIFVRSLVCQSLGLDANCVAKVAPSP"
     gene            2369..2758
                     /locus_tag="VGE59_03770"
     CDS             2369..2758
                     /locus_tag="VGE59_03770"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY1074787.1"
                     /translation="MLWQIGLFTSDGTKGYDPTTIGLPQIYELILRLGQILTYLIGAV
                     ILVYILMAALQYITAAGNDSKQAEAKKTITSAIVGFIIVLFAFTFINTLLRRIQFDAT
                     IIKGNVQTESGGNAGNQIDALPNSSSE"
     gene            2776..3171
                     /locus_tag="VGE59_03775"
     CDS             2776..3171
                     /locus_tag="VGE59_03775"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074788.1"
                     /translation="MKALVKFAPAIALAAYGVFATATPVFAQSANDLFGLQLSVYNGN
                     FQDALIGIINFILLAGGVLAFIFVLWGGFTYLSAGGDAAAAGKGRTMIVNAVIGLIII
                     FLSFSLVRFLTSRFDDNAGNSTFQDDDIK"
     gene            3181..3558
                     /locus_tag="VGE59_03780"
     CDS             3181..3558
                     /locus_tag="VGE59_03780"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074789.1"
                     /translation="MNPANVTIDISKLQAPIIQGPDLVSVLVGLLSWFLVIAGVVAFI
                     YALYGGFLYLTAGGDSAATVKARNTIINAVIGIVIIFISYSLVRFIIISLNVVQDNKI
                     KTINQQFPTPKPEDTGGVSEETE"
     gene            complement(4390..5925)
                     /locus_tag="VGE59_03785"
     CDS             complement(4390..5925)
                     /locus_tag="VGE59_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY1074790.1"
                     /translation="MADIDKAIYQFRRSDEESYTKAQAKHYHVPYVNLVGYPILPHTL
                     SLISVRDIESYKAVPFIRVGNKLRIAITTPTPETITFLQSIANQTGLEVSIALASPSS
                     INFGLHLAQLEDQKIAQAQHVAVSESQQKQALRAIGSRQELEKKLAQASATQVLDLLF
                     AAATGMEASDIHIEPQEHEVRVRYRIDGVLQEITNLTEASYHQILSRVKYLAHMKFDI
                     KNVNQDGRFTIELAETTLDVRVSTLPSAYGQIIDMRLLKAHAEFIKLDQLGLSPNSLS
                     LIKEAIALPHGMILVTGPTGSGKTTTLYAILDSLNQSGVKIITLEDPIEYRVAGIDQV
                     QVENDKGFTFAEALKGVLRQDPDIVMVGEIRDQETADIGLQAAMTGHLFLSTLHTNNA
                     PAALSRFVDMGIEPYKIAGAINLIIAQRLVRKRCANCQGEGCEACNQTGYKGRIPILE
                     VLKPSKALDDAIVRKAAVRELYEIAQKEGMVSLHDDGMEKVKQGLTTEEEVNRVTAEF
                     NQE"
     gene            complement(5930..6124)
                     /locus_tag="VGE59_03790"
     CDS             complement(5930..6124)
                     /locus_tag="VGE59_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074791.1"
                     /translation="MTPLKRPLFTTLIIIFSVGFIAILTWWLMFIVTNFRTLGTSVTA
                     PQAVATPNQSGLDSLIGEKD"
     gene            complement(6121..6615)
                     /locus_tag="VGE59_03795"
     CDS             complement(6121..6615)
                     /locus_tag="VGE59_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074792.1"
                     /translation="MIRRYIVVLSLILFVFAAITLGLQYFSNPALTTIKNRRQETERV
                     NSLMTANLTKESVLKQIEAGNGALREIFTSQSRLSPQITTIAQANTVQITNIVFPTNS
                     ESETAGSSLAQVSFVAPNHSKVTAFFKQLETGNPLLAIDEISLTPQQDGSYQGRITVR
                     FFTP"
     gene            complement(6612..8030)
                     /gene="pilM"
                     /locus_tag="VGE59_03800"
     CDS             complement(6612..8030)
                     /gene="pilM"
                     /locus_tag="VGE59_03800"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY1074793.1"
                     /translation="MPVFALTYRDNELSLLSQSGRGINRVSVALPEGCVHMGRIVDPE
                     KVSQVITQAVDLAQPKKISGKSLVIGLPEDQAFMKIIEMPSQLKDKELTTVLEHQWEN
                     FLPIPASQVYYDVVSLKTPGATRTSATQRVLVVAYPKEIITSILETTNLLGWTPVKFM
                     PLSFGMAQLFSVPNTGPTMVLGTSTGQDLSIAIVHNTVTHFSTTIHTAITDTKCYRQL
                     ENIRTYYEKNIASPGEKIAQIVILPSAYSDAMSKQIESFLLPTTVAPIEKALYINNPQ
                     QNFAVYIPLVGLLSSPVPLVLMPTEILDATETTRQIGLLQSMILGSVVILGVITYLTI
                     AFYGAIGFYQRHQTAYPDAIKERATRETTSLAESVTQFNQRVERLLKAKQERHLTTGE
                     VSSILATTLNNSKVTIRDISYSADKSTYTIKGSLLSSDDLGSLTEALKKEAVLKGAAI
                     TNEKSATPNDFTLFVTLQKAAS"
     gene            complement(8198..11497)
                     /locus_tag="VGE59_03805"
     CDS             complement(8198..11497)
                     /locus_tag="VGE59_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEY1074794.1"
                     /translation="MIKQHYPGSSIVAFLTITGLMLALLGTVFSLSFLTTAQATREQN
                     LRKAITLGNGALNHAIAKYRSDSNYRSESYALTTGAVDIALTNNESNSLTVNAQTYVP
                     YKQHSLAVCRSYQVDIDPITGDTIAGSYDESFACNKIPTDPTNTPPTLSIELPDSKGA
                     RAVAGQTYTVSYLLTDSQVDDVLTARFYYTPQNTSSSSKVEITGSCSIPLPGVPGSCN
                     WLIPANITPGDYYIYGEATDNVNPTVSAFSLGKLTILPVGTPAFTITRPANETPDIVT
                     STAGSSYSITYQLSDDYDGNTGQTVTANLFYDTDTNSSNGTSGSVSGCQGIPEGNLSC
                     NWATAGVPNGTYFIYGQTNGAQNNTSTYSVGKVTVTNNTAPQLSVTQPAGTPTVPSAQ
                     KTVTITFNLSDDDPVKVSFYYDKDAYNYNGTLISGCTDVSIQGQNQTCSWDVSQLPLG
                     DYWVYGVVTGDTYGRTTRAYAPGFVRISSVDAGTNAIATLAIHDPDGINDTVNQETNC
                     CASYQIRYSLNDPEETVTAAFYYATTATTTGGTAITTSNCAAAPEGTFSSCLWRIPTN
                     LSPGKYYIYGITTDVPNPADPNYNARLVKTLAPEPVIVAGILPACRDGRDNDGDGLTD
                     YPADKGCVSLDDSSEIDPPFNDYGTGVATDSSGDVYMAGYSDNYGLSNVFIRKYSKTG
                     QFCANASCYQSTTPWGDNGTSMIKITLPSTRLTKPTIAVDGNKNLFVAMAVGANSNAS
                     PYVMVYKYASNGTRVQTFGSNGSLTAPLNVGALRLRIDSNNNIYMLAKEDRSYDSRWF
                     ISKYSNNGVLCNTSTGPCGAWGTNGSGSISKDSNAPSQYAPSDIVIDTQGNLYLGGTA
                     FTSNPQQGTMNGFWHLRKYTATGILDTSFDGDGIVTYEKQVTVYRTGVGFDGNGNPVP
                     YQEELYTRNWAGLNALAVDATGNIYAGGAQVWEGGPYTQWVIRKYNGQGQLCSSTSNC
                     AWGENGRSGMTTYNHLLYGYDSDFVTGLTVTNTNQLIAIGYTDYSNATVRAYTSAGDV
                     NTSFNTDGTYGPFTNDQRQYDHVALTGTGTIVAIGNLQREYTKGMDALITTLTLSSNP
                     LTGEAYYDSTP"
     gene            complement(11490..12044)
                     /locus_tag="VGE59_03810"
     CDS             complement(11490..12044)
                     /locus_tag="VGE59_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY1074795.1"
                     /translation="MTKRQAGFTLIEVMVGLAITSFISVILLNASITGLQNFTFVSNA
                     QHRVSSFLEIFNRIDRHVRLAATFPTEYPELSSQPLYTANATTLIMKLHSVKSDGSRC
                     SNNFDYVIYTLENGKLREQVKTDPVSARAATDQTLLSNVSELLFTQTVKTRREVTVKL
                     TQQMLDGNRTVTRTNEQTMVARND"
     gene            complement(12037..12438)
                     /locus_tag="VGE59_03815"
     CDS             complement(12037..12438)
                     /locus_tag="VGE59_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074796.1"
                     /translation="MYKKLSGSILIESMITAAIVVVLLLLVSVSFIQTSQMSINSSQK
                     TIALGIIATTSEEDSQRDYEDTELSVGTKQFNSATKSTLSQLPEAIMTRTIVEKNGVK
                     ILQYLLEWKNNNHMLRTSAEYELTRKGIAND"
     gene            complement(12480..13682)
                     /locus_tag="VGE59_03820"
     CDS             complement(12480..13682)
                     /locus_tag="VGE59_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1074797.1"
                     /translation="MPNFRYTVLDPTGKRFTKLMAAANRNEVAKFLQQNKYFIIDITE
                     TTVRRINRSTPLNQRDKISFTQNIAVLLSSGISLGEAVTIVEQDSSSKSAASFYTSIR
                     NDLEQGTALSKALAHYPNLFDSVYISLIEAGENSGELSEVMNSLASSIEKDQRTLSQV
                     KSALLYPAFILASLVGLGVVLGFFVLPQITKVFAQIDTELPFATRMLVSLSNTLSSYP
                     IPILSGVMLLIAGILTYIRTAQGKKFISHLSTITPLIKPIILNLDLSRLSSTLALLLN
                     AGVPIQYAFKIAAGTLKNQRLRQELESTAEKLASGISLSQSLQTSSLPRTFVALVAVG
                     ERSGKVAAIFEGLGHHYEGQLDSALKNFTSLLEPMMTLFIGLLVGGVVITIMVPIYQF
                     VGNLQGAS"
     gene            complement(13700..15379)
                     /locus_tag="VGE59_03825"
     CDS             complement(13700..15379)
                     /locus_tag="VGE59_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY1074798.1"
                     /translation="MRLDDAAIQHLLWTEKLLTKEQIESAVLLAPSLGKSFVDTLIFK
                     SFISEDKLGEVIAESLGVEYTPLKGRTIATEVLDLISEESAVAHQIVPFDKNATELHL
                     AMADPTDFEIINFIEKKTGLIVRPHYAFPDQITIGLAQYKRDINTDLKKILNRVDDIG
                     TDLTKLAEDVPTIKMLDTIMEYAIVQNASDVHIEGQETNSLVRFRIDGLLHDILVLDK
                     KVQEAIVARIKYLSNLKIDEHRLPQDGRFKYEHIGNKVAVRVSILPAFYAENVVMRLL
                     SETSKTQTLEELGFAPHNIEIIVREIQRTNGIILLTGPTGSGKTTTLYSLIERLNKPE
                     VKIATIEDPVEYSVPRISQTQVNPASGLDFASGLRALLRHDPNIIMVGEIRDQETADI
                     SINAALTGHLVLSTLHTNDATSSIPRLIDLGVEPFLLAATIRVVVAQRLVRLLCSKCA
                     TPVQLTDAEVEELVNLTRFSADEIRSKTFFTPQGCSDCSDGYKGRLGIHEVLLVNETV
                     NKLIVAHPNNDILRTEAIKEGMYTMLQDGIAKAGSGLTTIQEVLREVGRQN"
     gene            complement(15382..16911)
                     /locus_tag="VGE59_03830"
     CDS             complement(15382..16911)
                     /locus_tag="VGE59_03830"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEY1074799.1"
                     /translation="MSRRHPAYFQWHNNSIAPIFHWASATLMLVITVGLVFSSYIPKT
                     QAAQLSWQGPEGFSSWQRTYTEIGNGLVLGQSPECCSYVTDGYASIQARPAPGMTIDW
                     QSSSLISMVNTEQSVVLSFKPQSCSGYLDHTTNADAQFSTDISTLPDSEFLCIRAHLT
                     TNNPNYSPALNSVTIDYVTVGTAPSPTPCPPNTPSDCGSAPANVCPGGTTVSVNADTH
                     VRADLPSTNFGGSAIYLANEDANGAPTSYTMYGLFRFPTQSISPNATTIYLTLPVYDT
                     TQTTSNGESITLQINRLITDPFNESTVTWNDLGSASLHEDTTYAYAKSTTFSRGSLSR
                     TTPLLAQLDVTQYVKDVQSGAIANTGFLVSGTSLDSMGSGNGSDFLLRIDPREDGLRP
                     AYVSWTGGCDTLPNGDPAPDPTPTQTSTFFETSTPTPTSTFFYSPSPTPTTTYFTLPP
                     YSPPPSPTPTSTFFYYPTPTPTWSDAFFYPTPTPTTTYPTTFPTANTGTPNPTTTFFV
                     D"
     gene            complement(17085..17474)
                     /locus_tag="VGE59_03835"
     CDS             complement(17085..17474)
                     /locus_tag="VGE59_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1074800.1"
                     /translation="MEKTLTGSNVLVVDDDAAIRDIYTRALKEAGATTLEAEDGEEGL
                     RMAREHHPSLILLDLMMPKRDGRAMLKDLKADESLKNIPVVIFSALITELEKDETIEA
                     GAAEYIEKSDIEEPEQLINKIKTVLHK"
     gene            complement(17540..18982)
                     /locus_tag="VGE59_03840"
     CDS             complement(17540..18982)
                     /locus_tag="VGE59_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1074801.1"
                     /translation="MKWLRSIRLKLAVMSTILLMVALFAQSAVTTVRLYKQERQQLIS
                     LAESQTAISVNPIVDTYLKYKDALGLELTRSMRTIAAQLPALDHFELIDTQGRVAFDS
                     RSLKHSVVLEPITNTVVLDRIQSNRASTLITAEGHTFIQPHFDSTGTHDYSIRSFVNF
                     SSLASMNRSNITQLIVTMLLLLPLVFFLSLWLTSQLILRPINEIRSTSNAIAGGDFDT
                     SIKTKSKDEFHLLAENINHMTETLIERVNSLEEEKAWKNEFLVLASHNLRTPLTVIMS
                     SVATLKKDSKISQESMKFVDYIYTRGKELHSLIENMLSISMLKAGKLQRQNEPFDLIP
                     LAQNALSGFEPRSKDKNVTMELETKSKHAIVSGDEGQILQVLDNLLDNALKFTPEKGK
                     ITLTIDDSKQDIFVTVKDSGEGIGQDMINKLFQSFHRGANPMSIDEKGSGLGLYFVKL
                     AVESHGGEVSIKSNAGHGTQVSFHLPKHAT"
     gene            complement(19009..19398)
                     /locus_tag="VGE59_03845"
     CDS             complement(19009..19398)
                     /locus_tag="VGE59_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1074802.1"
                     /translation="MTHTSPHTILIIDDNERLITSYRDFLQEAGLTITTATNGEEGLK
                     KAAQHKPDLILLDLMMPEMDGLTMLKTLKKDDTLKDIPVMVMTALVNELERDNSLKAG
                     AVEYIEKVETEPEQLLHKIRHLLKQAH"
     gene            19503..20984
                     /locus_tag="VGE59_03850"
     CDS             19503..20984
                     /locus_tag="VGE59_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1074803.1"
                     /translation="MKVPHLALSSSDRTEHVLGRRLRYILLGEIILIFVLFTLLLTVV
                     VAILSRQNEVNQMSISRFSMALEVQGVEQLLLKHMNYEQSYRLNSKQMVLDELLANHI
                     AFDQRITALSQKLQTLGPDEASFLPEEQKLSRRLAASHHEIILILEKMVGKSLTGSEH
                     NHSGLDSSSITIPLTPAGMEARISRLEEQSFADAGMWQQFVQADIDRLSYQSEVYQRL
                     RLTSLFGLACLMILVLVLVNYTYVLPSFNHILRHVVRQNKQLREVDSLKTEFLSVSSH
                     QLRTPLSELKWALTLLNRHQNKGDLTKQHHFVQQSLHSVDTMSQIVSALLNVSRIEQN
                     RLQFNPVVTDMAPVMRTVINRAKRAAKAKDVTLTFVSPSGGMKAHVDVLLVKQILQNL
                     IDNAIQYNRPHGKVAVIVKKQRASWLISISDTGSGISRKDLKHLFTKFYRGDNARRIR
                     PDGSGLGLYFVKKIVEKHRGKITVSSIEGHGTIFSITLPGVRG"
     gene            complement(21061..21537)
                     /locus_tag="VGE59_03855"
     CDS             complement(21061..21537)
                     /locus_tag="VGE59_03855"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY1074804.1"
                     /translation="MHKTLKGFTLIELIVVIGIIAILGAITIVAVNPARQFSQARNAV
                     RQSDTLAILNAVHQYAADNNGSFPATVAGLTADAAAEEICDSTKVTACPTGGIALNAV
                     LSPVAYISALPRDPQQTATTAQDTKYTIARSAANVITVCAPNALTDTVVKTICATR"
     gene            complement(21737..21824)
                     /locus_tag="VGE59_03860"
     tRNA            complement(21737..21824)
                     /locus_tag="VGE59_03860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21788..21790),aa:Ser,seq:gga)
     gene            21910..23727
                     /gene="dnaG"
                     /locus_tag="VGE59_03865"
     CDS             21910..23727
                     /gene="dnaG"
                     /locus_tag="VGE59_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY1074805.1"
                     /translation="MTDTVVEDIKSRLDIVDIIGNYVTLRQSGTNFKGLCPFHNEKSP
                     SFMANRERQIFRCFGCGEAGDIFAFVMKQENIDFPNALALLASKAGVELPEKFTKGRK
                     SGVEKSALYTVNDLATKFFNHILVQHPAGKEALDYLHKRGVKNELIERFQIGYAPSSN
                     LAIQQLFKKHDFTTDAVRLAGSPERYRDRIMFPFRDVIGQVVGYSGRALHDQVPKYLN
                     TAETDLFRKNRYVYGLFEAKKAIAEAKRVMLVEGQLDLVLAHQAGTAYAVATSGTALT
                     DDHLTILRRYSDTLLLAFDADSAGQKATERAISLALSHGFEINVVVLPAGKDPGEIIA
                     TDRSAWDRAVNKPLSAIEWLFGYYFPDISKRPSATERKKIYDALFPYVDKLADVVTRS
                     YALQRLAFSMGVTQEKALVDAFEEWKETPPSSEPSRRISSQPEQEKVGEPNQPAFVSE
                     EARREKSIVGIVLLHPDLLIHKQLHLEEKDFTNEALHHLYTTILSWYNKNTNQSNLSQ
                     LISSVETTLSAAARQSLQKLIFDTQTELAELTREQVVGEYTFLVKSVRQKQRDERMQH
                     YADEIRAAEAANDRLRVVQLMQEMQAALKEKELHAKENP"
BASE COUNT         6125 a         5034 c         5798 g         6779 t
ORIGIN      
        1 cctaatgttg cagcatgggg ttactatggt ggacacgcca accaatgtcg cggagtttaa
       61 tatttctgaa acgggtagcc gccctcctgg cacgcaagcg ctcgactact ttacgagctt
      121 tggaaaagtt ggaaatgcga attatacctg gcaacgtaat tttggaactt cgctcgatgc
      181 tcttaaagcg ggaaaaacct ttatggcggt tgggtatctt aatgatatgg ctggattagg
      241 tgctctcggt caaacgaata tcggagttgc cgcgctgcca caagtagacg taagtaaccc
      301 tcaaacatac ggccgatatc tcgctgcgac agtaacgaaa caggctgctc aatcttcgaa
      361 ggggcgtgac gagaagaagc tccaagcagc ctgggacttt gtggctctct ttgccaaccc
      421 atatgtgagt gactcgtatg ccaaatcact ccgcgtcgtt ccagcccgta aagatgtggc
      481 caagaaacag acctttggtg agtattatga tccctttatt tctcaagttc cgtatgcagt
      541 aacttggaac aaagatgagg tagcggttgc tgatggggcg ctcaaagagg cattaaccct
      601 tgcgatcgac aaaaaacagg attctcaggt tgcgttagac gtagcggcta agggatatac
      661 gtcattcttg caacgcgacc ggggtattaa tactgatgaa aaggttatga acttttggat
      721 gtctgaagac gataccacgg tttataaaga gacgatccga aactacttag gtgagaataa
      781 agatattaaa cgtattgcaa catcaaagcg gagtcccgcc cgctttgaat gggaaattct
      841 taatgcaatg gctgcccgta agggacccga ccttcttgct ctcccaagtg atgcagtggt
      901 acgttactcc ccacttcttc gcgggttgcc actcaaagga tttaataaat cgactcgaat
      961 ctctgataca gaagctttga atctttctta ttctccggct gtaaaaacag ataactacat
     1021 caatggtaag ctctatggta tgccagcaca gatagaaacc cttatgatcg gatataactc
     1081 taacttacag aatcaagtcc gtcgtgcgtg gcaagacaag aactttgaaa cagatccaac
     1141 tgacgaccca ttctcttccg agcttatatt gtggaaagat ttaaaaagaa gggctgagat
     1201 cacgagccaa gttatgcagg atgtacgtta tattgctctg ggaacaggat cgaatgttga
     1261 gcatagtgcc gatatttttg ccgcgatact caaacagtat ggtggtgaga tgactgatcc
     1321 ggatagaaat gttaccggta ttcaccttcc tatttcgagc cgccaaacag ttgttcctgg
     1381 tcaagaggcg gaaaaactta ttcgtagtta tgccgacaca agtagtgaat ggaactcttc
     1441 tttaccaaat tctctccagg ctctggcgga aggaaaagta gttgctgcct ttatttaccc
     1501 ccgagatatc gcaaaaataa aagcactgaa tccagaactc tctgttagtt acattccgtt
     1561 gcctcagtta acggatactg gtgacccaat tgacttttct tcttcctata gtcttacggt
     1621 tccgttagcc tccaagaaag ccgccaaggc atacaatttt attgtcacga gcgtctttga
     1681 cggaggacaa aacgataact taatccctcc aacccttggg agattaaaaa cccctattct
     1741 ggagaggcga cgggggggca gtgtgcagac aatgcaacga aatgcagctc agagttacta
     1801 taaaggtggt caccctaaag agatagatca agcaattatt gactttcttg accgaaaacg
     1861 aacactcgat cagacggcga atgtaattaa tcagcttctc aaaaagaacg tagcggacta
     1921 aggatgaaga acctattctc ttgcctcagt atgagcttct tatctttgat tgttggggtg
     1981 caaactgctt ctgcccagtt cctttttaaa aggcctaccg caactcctcc tcccattaaa
     2041 ctgcctaatt ttttaccgaa tatatcgatt gatccagaaa ctattgcgca gagtattctt
     2101 aaccttgttg tgacttggat tacgttgatt gctggtattg cggcttttgt ttacattctc
     2161 tatggcggct ggaaatatat cacatcagca ggaaacccgt cagaggcaca agtcgcaaaa
     2221 aagatgatta taggcgcgat tattgggtta cttattattg ccttggccta tattgttctg
     2281 atctttgttc gtagtttagt ctgtcaatct cttggtttag atgcgaactg cgttgcgaaa
     2341 gtggctccca gtccataagg agaaacatat gctgtggcaa attggccttt ttacaagtga
     2401 tggaacgaag gggtacgacc caacaacgat tggtttacct caaatctacg agctgatttt
     2461 acgcttggga caaattttga cctatctcat aggtgctgtt atcctagttt acattcttat
     2521 ggctgcgcta caatacataa ctgcagcggg gaatgatagc aagcaagccg aagctaagaa
     2581 aaccataaca tcagcaatcg ttggatttat tattgttctc tttgctttta cgtttataaa
     2641 taccctcttg cgccgcattc aatttgacgc tacgataatt aaaggtaacg tacagactga
     2701 atcagggggt aatgcgggaa atcaaataga tgcattgcct aacagttcgt ctgaataatt
     2761 taaggaagga gatttatgaa agctctcgtt aagttcgcgc cagctattgc cttagcagct
     2821 tacggcgtat ttgcaacagc gactcccgta ttcgcgcaaa gtgcaaatga tctgttcggt
     2881 cttcagttaa gtgtctataa tggtaatttt caagatgcat tgatcggaat tattaacttt
     2941 attctcttgg ctggtggtgt tcttgctttt atctttgttc tctggggtgg cttcacatac
     3001 ctctctgcgg ggggtgatgc agcagcagca ggtaagggac gtaccatgat cgtgaacgct
     3061 gttattggtt tgatcattat cttcctatcc ttctcgctcg ttcgtttcct taccagccga
     3121 ttcgatgata acgcaggtaa tagcaccttc caagacgacg acattaaata agcttggata
     3181 atgaacccgg caaatgtaac gattgatatc tcaaagcttc aggcccctat tattcagggg
     3241 cctgatctcg taagcgtctt agttgggctg ctgagctggt ttttggttat tgctggtgtt
     3301 gtcgcgttta tctatgccct gtacggaggt tttttatacc ttacggcagg gggcgatagt
     3361 gcggcgacag taaaagcacg aaacacaatt atcaatgctg ttattggcat tgtgattata
     3421 tttatttctt actctctggt tcgttttatt attattagcc tgaacgtcgt acaagataat
     3481 aagataaaga caatcaatca gcaatttcct actcctaaac ctgaagatac gggaggcgtg
     3541 agcgaagaaa ctgagtaagt tttttaatct tagttggctt tttagtgtaa cagagaggta
     3601 ttttacctct ctgttacttt attacgattg acaaattatc aattttatga tatattccat
     3661 tcaaatcttc gaagcaacgc gattgataaa ggagtgtgag atgttgcgtt tgatgatggc
     3721 tctagtactt gccctcacct tgaccggtgt tgtgcgggcg gatgagccgg aagacctgaa
     3781 gccggcgatg gagcaggtta tggctccttc tacgccggtc acacccccgg tggtaccggt
     3841 tccgccgacc agtggcgctc aggtgcccgg ctcgcatgtc gggatcccaa cggtagcccc
     3901 ggtgggagtc gcgccgcaca accccgcgtt gtgcgaagcc tgccggtact accacacggt
     3961 cgtcccggcc accgtccttc ctcagcacaa cccgctgaca tgccccgagt gtcggtttta
     4021 ccaccgacaa gcggagatga tgcgttctcc ccaggtggag gcgaatcggc gcgatcctcg
     4081 tcctcgccag gatgatcgac catactatgg tccgtacatc tcctggtggc aggatcggag
     4141 taagccgacg gcctacacgc cggccgacgt cgaggcgggt cgccgccaag agtatcagga
     4201 gcggtacggt cgagctccat acgtcggtgt caatcgtccc gtcaagcggg acgcgccgac
     4261 aacctacacc cccgcagatc tagaggcccg ccggcgccaa caggcacaag agcggcgcta
     4321 ctagcctgaa agggggctct cgacacgaag aagccagcgc gtaagcgctg gctgcttttt
     4381 ttcttagaat tattcttgat tgaattcggc ggtaacccgg tttacttcct cttcagttgt
     4441 gaggccttgt tttacttttt ccattccgtc gtcgtggagc gaaaccatac cctctttttg
     4501 agctatttca tagagctctc gcacggcggc cttacggaca attgcatcgt cgagggcttt
     4561 ggagggcttt aagacttcga gaataggtat acggccttta taaccggttt gattacaggc
     4621 ttcacaaccc tccccctggc agttcgcgca gcgtttacga actaaccgtt gggcaataat
     4681 aaggttaatg gcgccggcaa tcttatacgg ttcgattccc atatcgacaa agcgagacaa
     4741 ggctgctggg gcattattcg tatgtaaggt cgataagaat aaatgtcccg tcatagccgc
     4801 ttggaggcca atatcggctg tttcttggtc gcggatttct ccaaccatca caatatcagg
     4861 gtcttgacgt aaaacacctt tgagagcttc ggcgaaggta aaccctttgt cgttttcgac
     4921 ttggacttga tcaatcccgg ctacccggta ctcaattgga tcttcgaggg taataatttt
     4981 gactcccgat tgattaaggc tatcgaggat ggcatagagc gtggtggtct ttcccgatcc
     5041 ggtgggtccg gtcacaagaa tcatgccgtg tggaagagca atagcttctt taataagaga
     5101 gagggagttt ggtgaaagcc cgagctgatc cagtttaata aactcggcgt gtgcctttaa
     5161 gagacgcata tcaatgattt gaccataggc tgaaggcagt gttgaaacac ggacatcgag
     5221 agtggtctcg gccaactcta tggtaaaacg accgtcttga ttgacgtttt ttatatcgaa
     5281 tttcatgtgc gcaagatatt ttacgcgaga aagaatctga tggtacgacg cttctgtcag
     5341 gtttgtaatt tcctggagga cgccgtcaat acggtaacgt actcgaactt catgttcttg
     5401 gggttcgatg tgaatatccg aagcttccat gcccgtggca gcggcgaaga gtaaatcgag
     5461 gacttgagtt gcagaggctt gggcgagctt tttttcaagt tcttggcgtg agccaatggc
     5521 tctaagtgcc tgtttttgtt gtgattcgct taccgccaca tgttgagctt gtgctatctt
     5581 ttgatcctcg agttgagcaa gatggagacc aaaattgata ctcgaaggtg atgcgagtgc
     5641 aatgcttacc tcgagcccgg tttggttggc aatactctgc aaaaaggtaa ttgtctctgg
     5701 cgtgggagtg gtaatcgcga ttcgtaactt attgcccaca cggataaagg gtaccgcttt
     5761 atagctttca atgtctctga ccgaaattaa gctaagcgta tgaggtaaaa ttggatatcc
     5821 cacgaggttt acatacggca catggtagtg ctttgcttgt gcttttgtat agctttcctc
     5881 atcgctgcga cggaattggt aaattgcctt gtcaatatca gccatagaat taatcctttt
     5941 cccctatgag cgaatcaagt cctgattggt tgggagtagc tacggcctgc ggtgcagtta
     6001 ctgatgtacc aagcgtacgg aagttcgtta ctataaacat gagccaccaa gtcagtatgg
     6061 cgataaagcc aaccgagaat ataataatta aagtggtgaa gagaggtcgt ttgagggggg
     6121 tcatggcgta aaaaacctta ctgttatgcg gccttggtaa ctcccgtcct gttgaggggt
     6181 aaggcttatt tcgtcgatag caagcagagg attaccggtt tcaagctgtt tgaaaaaagc
     6241 agttactttt gaatgattag gagcaacaaa actaacttgg gcaaggctac ttcccgctgt
     6301 ctcagattct gaattcgttg gaaatacaat atttgtaatt tgaacggtat tcgcctgggc
     6361 tatcgttgtg atttgtgggc tgaggcggct ttgggaggtg aagatttctc gcagtgctcc
     6421 attgcctgct tctatctgtt taagcacaga ttccttggtg aggtttgccg tcataagtga
     6481 attaacccgt tcagtctctt gtcggcggtt tttgattgtt gtgagtgcag gattggaaaa
     6541 gtattgcaac ccaagtgtaa tagcagcaaa tacgaataga atcagactaa gtacgactat
     6601 atagcgacga atcatgaggc cgccttttga agtgtgacaa agagagtaaa atcgtttggt
     6661 gtcgctgatt tttcattcgt gatagcggcc cctttgagta cagcttcttt ttttaaggct
     6721 tcggttaaac tacccaaatc atcactcgat agaagacttc ctttaatagt gtaggtagac
     6781 ttgtctgctg aataactgat atcccgaatg gtgaccttcg agttatttag ggttgtcgcg
     6841 agaatactgg agacttctcc agtggtgaga tgacgttcct gctttgcttt tagcaagcgt
     6901 tcaacccgtt gattaaattg ggtgacggat tcagcaaggc tggtagtctc acgggtcgca
     6961 cgttctttaa tggcgtcagg gtaggctgtt tgatgacgct gataaaaacc aattgcccca
     7021 taaaaggcga tggtgagata cgtgatgaca ccgagtataa cgacgctccc aaggatcatt
     7081 gattgtaaaa gaccaatttg acgggttgtc tctgttgcat ccagtatttc ggttggcatg
     7141 agaacgagag ggaccggtga agaaaggagg ccgacaagag ggatatagac cgcaaagttt
     7201 tgctgagggt tgtttatgta gagggctttt tcaatcggtg ccaccgttgt agggaggaga
     7261 aatgattcaa tttgtttact cattgcatca gagtacgcag aaggtaaaat aacaatctgt
     7321 gcaatttttt ctcccgggct ggcaatgttt ttttcgtaat acgttcgaat attttctaat
     7381 tgtcggtaac acttggtgtc tgtaattgcc gtatggatcg tagtcgaaaa gtgagtcacc
     7441 gtattatgaa cgattgcaat cgagaggtct tgtcccgtac ttgttcccag aaccatggtt
     7501 ggtccggtat tgggcacaga aaagagctgg gccataccaa atgataaagg cataaacttt
     7561 acgggtgtcc atccaagtaa attggtagtt tccagaatgc ttgtaatgat ctctttcgga
     7621 tatgcgacaa ctaagactcg ttgtgtcgct gaagttcgtg ttgctcctgg agttttgaga
     7681 gacaccacat catagtaaac ttgtgaggct ggaataggta aaaagttttc ccattgatgc
     7741 tccaaaacag ttgtcagttc cttatctttc agctggcttg gcatttcgat tattttcatg
     7801 aatgcctgat cttcaggtag tccaattaca agacttttgc cactaatttt ttttggctgc
     7861 gccaaatcta ccgcttgggt aatcacctgg cttacttttt ctgggtcgac aattcgaccc
     7921 atatggacgc agccttctgg gagggcaacg ctgacacgat taatcccccg tccactttga
     7981 gacaggaggc taagttcgtt atctcggtac gttagtgcaa aaacaggcaa gcccctcctt
     8041 tacttcaact ccttcttttc tctcaatacg aagaaagcag aagtcccctc tttacctact
     8101 atagcgcagg ccgtgcgtgc gcgctaccca ttataagcgc ctactttttg tttggatggt
     8161 ttgcttttgg atgtgtattc tgagggtgta acaagactta aggagttgag tcgtagtagg
     8221 cttctccggt aagcgggtta gatgacaggg ttagcgtggt aattaatgca tccatacctt
     8281 ttgtgtactc cctctgtagg tttccgatcg caacgattgt tcccgtgccc gtgagtgcaa
     8341 catggtcata ctgccgctga tcattcgtaa acggtccata ggttccatca gtgttgaaag
     8401 aggtatttac atctccagca gaggtgtatg cgcgaaccgt tgcattagaa taatcggtat
     8461 aaccaattgc aatgagctga ttcgtgttcg tgacagtgag cccggttaca aagtcgctgt
     8521 catacccgta aagtagatgg ttatacgttg tcatcccgct gcgtccattt tcaccccatg
     8581 cacaattgga cgtactgcta cataactgcc cttggccatt atacttgcgt attacccact
     8641 gggtatacgg cccgccttcc caaacttgtg ctccaccagc atagatgttt cctgtcgcat
     8701 cgactgctaa cgcatttaaa ccagcccagt tacgggtata gagctcttcc tggtagggaa
     8761 ctgggtttcc attcccatcg aagccgactc ctgtccgata cactgttacc tgtttttcat
     8821 aggtaacgat gccgtcacca tcgaagcttg tatccagtat ccctgtagcc gtatattttc
     8881 gaagatgcca gaaaccattc atggttccct gttgaggatt agaggtgaat gctgtccctc
     8941 caagataaag gtttccttgt gtgtcaatca caatatcgct tggtgcatat tgggaaggtg
     9001 cgttactatc tttgctaatt gatcccgacc cattcgttcc ccaagctcca catggccctg
     9061 tactcgtatt acagagaaca ccattatttg aatacttact aatgaaccat cgagaatcat
     9121 acgatcgatc ttcttttgcc aacatgtaga tattgttgtt gctatcaatc cggagacgaa
     9181 gggcgccaac attgagtgga gcagttagag aaccattcga cccgaatgtc tgtactcgcg
     9241 taccgttgga agcatattta taaaccataa cataggggct tgcattggaa tttgccccaa
     9301 ctgccattgc tacaaaaaga tttttgtttc catcgacggc aatggttggt tttgtcagtc
     9361 tggtcgaagg cagggttatc ttaatcatgc tcgtaccatt gtctccccaa ggtgtggtgc
     9421 tctggtagca tgatgcattg gcacagaatt gtcccgtttt gctatattta cggatgaaaa
     9481 cgtttgagag gccataatta tctgagtaac ccgccatgta cacgtcacca gaactatcgg
     9541 ttgcaactcc cgtgccataa tcattaaaag gtgggtcaat ctcgctagaa tcgtctaagc
     9601 tgacgcaccc tttgtcggct gggtagtcag ttaacccatc gccgtcatta tctctgccgt
     9661 ctcggcaagc aggtaatatt cccgcgacaa taactggctc aggagcgagt gtttttacga
     9721 gtcgggcatt gtagtttggg tcggcgggat ttggcacgtc agttgtaatg ccgtaaatat
     9781 agtattttcc ggggctaaga ttggttggaa tgcgccagag acagctagaa aaagtgcctt
     9841 cgggtgctgc tgcacagttg ctagttgtga tcgcggttcc ccctgtggtg gtagccgttg
     9901 tcgcgtaata aaatgctgcg gtaactgttt cctcggggtc gttcagggaa tagcgaatct
     9961 ggtaactggc acaacagttc gtttcttggt taacggtgtc gtttattccg tctggatcat
    10021 ggattgcaag tgtggcaatt gcatttgttc ccgcatcgac ggacgagatg cgaacaaagc
    10081 caggggcata tgcacgagtt gttcgaccgt atgtgtcgcc cgtcaccacg ccgtagaccc
    10141 aatagtcacc gagcggtagc tgtgagacat cccagctaca cgtctggttt tgcccctgaa
    10201 tcgaaacatc ggtacaaccc gaaatgagcg tgccattata gttgtatgca tctttatcat
    10261 agtagaaact gactttaaca gggtcatcgt cacttagatt aaaagtaata gtaactgttt
    10321 tttgcgcact tgggacggta ggcgtccctg ccggttgtgt gacggatagt tgaggcgcgg
    10381 tattgtttgt aacagtgact ttccctactg aataggtcga cgtattgttt tgtgcgccgt
    10441 tcgtctggcc gtagatgaaa tatgtgccat tgggtactcc ggctgttgcc caattgcacg
    10501 aaagattacc ctctgggata ccttggcatc ctgacaccga accgctcgta ccattgctgc
    10561 tgtttgtatc ggtatcataa aacaaattag ccgtaacggt ttgtcctgta ttgccatcat
    10621 aatcgtctga taattgatag gtaatagagt atgaagaacc tgcggtactc gtcacaatgt
    10681 cgggagtttc attggctggt cgggtaatcg taaaggctgg tgttccaaca ggcagaatcg
    10741 ttagttttcc cagagagaaa gcggagactg tgggattaac gttatcggtc gcttcaccat
    10801 aaatatagta atcacccggg gttatatttg ccggtataag ccagttgcag gacccaggga
    10861 cgccgggtag aggtatcgaa caggaaccag taatttctac ttttgaagag gaagaagtat
    10921 tttgaggggt gtagtagaaa cgggctgtca gtacatcatc gacttggcta tcagttaaga
    10981 ggtaagaaac agtatatgtt tgccctgcaa cggcccgtgc acctttgctg tcggggagtt
    11041 ctatggaaag ggttggcggt gtattggtcg gatctgtggg aatcttgtta catgcaaaag
    11101 actcatcata acttcctgct atcgtatcac ccgtgattgg atcgatatcg acttgataag
    11161 agcggcaaac tgctaggcta tgttgtttgt aggggacata tgtttgggca ttcaccgtta
    11221 ggctgttact ctcattattt gttaaggcga tatcaacggc cccagtggtg agggcgtagc
    11281 tttctgatcg atagttgctg tcgctacgat atttcgcaat ggcatggtta agagcgccat
    11341 tccccaaggt gatggctttt cgtaaattct gttcgcgagt tgcctgagcc gttgtgagaa
    11401 aagagaggga aaaaacagtt cctaagagag cgagcatgag acccgttatt gttaaaaagg
    11461 caacaattga agatccagga tagtgttgtt taatcattgc gtgccaccat ggtttgctca
    11521 ttggtgcggg ttacggtccg attgccatct aacatttgtt gtgtcagttt aacggtgact
    11581 tcacgacgag ttttaacggt ctgtgtaaag agaagttccg atacgttcga aagaagcgtt
    11641 tggtctgttg ctgctcgagc tgaaacagga tctgttttga cttgctctcg tagctttcca
    11701 ttttctaagg tataaatcac gtagtcaaag ttattggagc aacgagagcc atctgattta
    11761 acactatgga gcttcataat taaggtcgtc gcattggccg tatagagggg ttggctactg
    11821 agttcaggat actcagtagg aaaagtagcg gcaagtcgaa catgacgatc tatacggttg
    11881 aatatttcta agaatgaact gacccgatgc tgggcattac tgacgaacgt aaagttttgg
    11941 aggccagtaa tcgaggcgtt aagtaaaata accgaaataa atgaagttat tgctaaccca
    12001 accattactt caattaaagt aaatcctgcc tgacgcttag tcatttgcaa ttcccttccg
    12061 ggttagctcg tattcggcgc tggtgcggag catatggttg ttatttttcc attccaggag
    12121 gtattgaaga atctttaccc cattcttttc tacgattgtg cgggtcataa ttgcttcggg
    12181 caactgtgaa agagtggatt ttgtggccga gttaaactgt ttagtaccaa cgctaagttc
    12241 tgtgtcttcg taatctcgtt gagagtcttc ttctgaggtc gtggcaataa tccccagtgc
    12301 aattgtcttt tgacttgaat taatgctcat ttggcttgtc tgaataaaag agacagatac
    12361 taagagtagt aaaacaacga caatcgccgc agtgatcatg ctttctataa ggattgatcc
    12421 ggacagtttt ttatacatac tcttcttcta ggttactggt ctctcattgg actgtcaatt
    12481 taagaagcgc cttggaggtt gccaacaaat tgataaattg gaaccataat agtaatcacc
    12541 actccaccaa ccaacagacc gataaagagt gtcatcattg gctcaaggag gctggtaaag
    12601 tttttaaggg ccgagtcaag ttggccttca tagtggtgcc ctaaaccttc gaaaatagcc
    12661 gcgaccttac ctgatcgctc accaacggca accaaagcga cgaaggtccg ggggagtgag
    12721 ctggtttgga gagactgact cagtgagata ccactcgcaa gtttctcggc ggttgattca
    12781 agttcttgac gcagacgttg gtttttgaga gtcccagccg caattttaaa ggcatattga
    12841 atcgggacac ctgcgttaag aagtaatgct aacgtagacg aaagacggga aagatcgaga
    12901 ttaagaataa tgggcttaat aaggggtgta atagttgaaa ggtgactaat gaatttttta
    12961 ccttgtgccg tgcgaatata cgtgagaatt cctgcgataa gaagcatcac cccacttaaa
    13021 atggggatgg gatagctgct caatgtgttg ctcaaggaga ctaacatgcg tgttgcaaag
    13081 ggaagctcgg tatcaatttg agcaaatact ttcgtgatct gtggcaaaac aaagaaccct
    13141 aatactactc caagaccgac taacgaagct aaaataaaag cgggataaag aagagcgctt
    13201 tttacttggc ttaaggtccg ttgatctttc tcaatactcg atgccaaact attcatgact
    13261 tccgacaatt cgcctgagtt ttcgcccgct tcaatcaagg aaatgtacac agaatcaaat
    13321 aagtttggat agtgagccaa cgcttttgat agagccgtac cttgctctaa atcgttgcgg
    13381 attgatgtgt aaaaactggc tgctgatttg cttgagctat cttgttccac aattgttaca
    13441 gcttctccaa gagaaatgcc tgagctcaag aggacggcaa tgttttgcgt gaagctgatt
    13501 ttatcgcgtt gattaagagg ggtacttcga ttgatacgcc ggacggttgt ttcggtaata
    13561 tcaataataa aatacttgtt ctgttgcagg aacttcgcaa cttcattccg gttggctgcg
    13621 gccataagtt tggtgaatcg cttgccagtt ggatctaaaa cagtgtaacg aaaattaggc
    13681 atgagtgatc acattctctt taattttggc gtccgacttc acgcaatact tcttgaattg
    13741 ttgtcagtcc tgaaccagct tttgcaatgc cgtcctgtag catggtatac ataccttctt
    13801 taatagcttc ggtccggaga atatcattat ttggatgggc cacaattaac ttattaacgg
    13861 tttcgtttac taataagact tcatgaatgc cgagccgtcc tttgtatcca tcgctgcagt
    13921 cgctacagcc ttggggagta aagaaggttt ttgatcgtat ctcgtcggcg gagaatcgag
    13981 ttagattcac gagctcctct acttccgcgt cagtgagttg aaccggggta gcgcacttgg
    14041 agcagaggag ccggacgagc cgttgtgcaa caaccactcg aatagtggcg gctaacagaa
    14101 atggttcaac gccaagatca atcaagcgag gaatagaaga tgtcgcatcg ttggtatgga
    14161 gagttgagag aactaagtga ccggtaaggg cggcgttaat gctaatgtct gccgtttctt
    14221 ggtctcgaat ttcacctacc atgatgatat ttgggtcatg acgtaggagt gctcgtaatc
    14281 ccgaagcaaa atctagtcca ctcgcagggt tgacttgggt ctgagaaata cgtggcacgc
    14341 tgtattcaac gggatcttca atagttgcaa tttttacttc cggcttattg agacgttcaa
    14401 ttaagctgta gagcgttgtg gttttccctg atccggtagg tccggttaac agaataatcc
    14461 cgttggttcg ttgaatctct cgaacaataa tttcgatatt atggggtgca aaaccgagct
    14521 cttcgagagt ctgagttttg ctagtttcag agaggagacg cataaccacg ttctcagcgt
    14581 agaaggctgg taaaatcgat acgcggactg cgaccttgtt gccaatatgt tcatatttaa
    14641 accgaccatc ttgaggaagt cggtgttcgt caatttttaa gttcgataag tatttaatac
    14701 gggcaacaat cgcttcttgt acttttttgt cgagaaccag aatatcgtgg agcaggccgt
    14761 cgattcggaa ccggacaaga ctatttgttt cttggccttc aatgtggacg tcactggcat
    14821 tctgaacgat ggcatattcc ataattgtgt cgagcatctt aatcgttggc acgtcttcgg
    14881 ccagcttggt taggtctgtt ccaatatcat cgacgcggtt aaggatcttt ttcagatcag
    14941 tattaatatc acgcttatac tgagctaacc cgattgtgat ttgatcggga aaagcgtaat
    15001 gaggacggac gatcagccct gtctttttct cgataaagtt aataatctcg aaatcggttg
    15061 ggtccgccat ggccaaatgg agctcggtag catttttgtc aaatggcaca atctggtgag
    15121 caacggcaga ctcttctgaa atgaggtcga gaacctctgt tgcgatagta cgacctttga
    15181 gaggggtata ttcaacgccc agagattcgg caatgacttc tcctaattta tcttctgaaa
    15241 taaagctttt aaaaatcaga gtatcgacaa acgatttacc aagggaaggc gcgagtaaaa
    15301 cagccgattc aatctgctct ttcgttaaga gcttttcggt ccacagaaga tgttgaatgg
    15361 ctgcgtcatc aagtctcata tttaatccac aaagaaggta gttgttgggt tgggcgttcc
    15421 cgtgttggca gtgggaaagg tagttgggta cgtcgtagtt ggtgttggcg tcgggtagaa
    15481 gaaagcatca ctccatgttg gtgtcggagt ggggtaatag aagaacgtgc tggttggcgt
    15541 gggacttggc ggtggtgaat aaggaggcag tgtaaaatat gttgtagttg gagttgggct
    15601 gggagagtag aagaaagtac tcgttggggt cggtgtactt gtttcaaaga aagttgaggt
    15661 ctgcgttggc gtagggtctg gggcaggatc cccgttgggt aaggtgtcac accctccggt
    15721 ccatgataca tacgcgggcc gcaacccatc ttcacgaggg tcaattctta ataagaagtc
    15781 agagccgttg ccactgccca tactgtcgag tgatgtaccg gagaccaaga agcctgtgtt
    15841 cgcaatcgca ccactttgaa cgtctttgac gtattgagtg acatcaagtt gagctaaaag
    15901 tggtgtcgta cgagataaag aaccacgaga gaaggttgtg gatttggcgt aggcatacgt
    15961 cgtgtcttcg tgaagcgatg cactgcctag gtcattccaa gtgacagttg attcattaaa
    16021 tggatccgtg attaagcggt ttatttgaag cgtaatcgat tctccgttac tggtagtctg
    16081 cgtcgtgtcg taaaccggga gggtgaggta gatcgtagtc gcgtttggag atatgctttg
    16141 ggtcgggaaa cgaaataggc catacatggt ataacttgtc ggtgcgccgt ttgcatcttc
    16201 gttcgcaagg tagatagcgc ttcccccaaa atttgtgctc ggcaaatctg cccgcacatg
    16261 ggtatcagca ttcaccgaaa cagttgttcc tccgggacaa acattagccg gagcgctccc
    16321 gcagtcgctt ggcgtattcg gggggcaggg cgttggagat ggagccgttc ccacagtaac
    16381 gtaatcaata gtcaccgagt taagagccgg gctatagttc ggattattag ttgtaagatg
    16441 agcccggatg cagagaaatt cggaatcagg gagcgttgag atatctgtac taaactgtgc
    16501 gtcagcattt gttgtgtggt ctaaatagcc gctacaggat tgaggtttaa aactcaatac
    16561 gaccgattgc tcggtattga ccatgcttat aagggaagat gattgccagt caatggtcat
    16621 ccctggtgca ggacgagctt gtattgaggc atatccgtca gttacgtagc tacaacattc
    16681 tggagattgg ccaagtacga gtccattacc aatctcagta tatgtccgtt gccaacttga
    16741 gaatccttct ggtccctgcc acgatagctg ggctgcttgt gttttaggta tgtaactcga
    16801 gaaaacaagt cccactgtta tgacgagcat aagggtggcg cttgcccaat gaaaaatcgg
    16861 tgcgatcgag ttattgtgcc actggaaata agccggatga cgccggctca accattcgtg
    16921 atgtacctga agccaaggga ttaatgtctt cggggtcgtg tcacgtattg caccctcctt
    16981 atctatcgct ccgaaaagcg aagagttcct cttatctact ataacaaaag agagcgtacc
    17041 aggcctagcc tgttggctct cttttgttag gaatatgatc ccttttattt atgtaaaacg
    17101 gttttaatct tattaatgag ttgttctggt tcttcgatat cagacttctc aatatattca
    17161 gcggcgccgg cttctattgt ctcgtctttt tccaattcgg tgatgagggc gctaaaaata
    17221 acaacaggaa tattttttaa gctttcatcg gctttaagat ctttgagcat tgctcgtcca
    17281 tctcgtttcg gcatcatgag gtcaagtaat atgagagaag ggtggtgttc tctcgccatc
    17341 ctgagcccct cttctccatc ttctgcttcg agtgttgttg cgcccgcttc tttgagggct
    17401 cgagtataga tgtcccgtat tgcggcgtca tcatcgacta cgagtacgtt tgatcctgtg
    17461 agagtctttt ccattatcgc tccttttgtc ttattcaaca ctgctcccgt aaaaaaggca
    17521 agggaaatga ggtgttacac tacgtggcgt gtttggggag atgaaagcta acttgcgtcc
    17581 catgtcctgc attactctta attgaaacct ctcctccatg tgattcgacg gcgagtttta
    17641 caaagtaaag acctagcccc gaaccttttt catcaataga catggggttt gcgcctcggt
    17701 gaaacgattg aaagagctta ttaatcatat cttgtccgat gccttctccc gagtctttca
    17761 ccgtaacaaa gatatcttgc tttgagtcgt cgatcgtgag cgtaatctta cctttttcag
    17821 gagtgaattt cagagcgtta tcgagcagat tgtccaaaac ttggaggatt tggccttcgt
    17881 ctccactcac aatcgcatgt ttcgattttg tttctaattc cattgttaca tttttatctt
    17941 tacttcgtgg ttcgaatccg ctcagggcat tttgagcgag agggataaga tcgaacggtt
    18001 cattttggcg ttgtagtttt cccgctttaa gcatggaaat ggacagcatg ttttcaatga
    18061 gagagtgtag ctctttgcca cgggtataaa tgtagtcaac aaatttcatg ctctcttgag
    18121 aaatttttga atctttctta agggttgcaa ctgaactcat gatgactgtg agaggcgttc
    18181 ttaagttgtg cgatgctaaa actaaaaatt catttttcca agccttttcc tcttccaggc
    18241 tgttgaccct ctcaatcaaa gtctctgtca tatggttaat attttctgcc aagagatgaa
    18301 attcatcttt ggacttggtt tttatggaag tatcaaaatc ccctcccgca atcgcgttac
    18361 tcgtagaacg tatctcgttt atcgggcgca gaataagttg gcttgtaagc caaagactta
    18421 agaaaaacac aagaggtaag agcagaagca tcgttactat aagttgggta atattagaac
    18481 ggttcatgct tgcgagtgaa gagaagttca caaagcttcg tatggagtaa tcgtgtgttc
    18541 ctgtactgtc gaagtgtggt tgaataaagg tatgaccttc ggccgttata agtgtgctcg
    18601 cacggttaga ttgaatccta tctaagacaa cggtgttggt gatgggttct aagacgacag
    18661 agtgtttcag agaacggcta tcaaacgcca ctctcccctg cgtatcaatg agttcgaagt
    18721 gatccagtgc cggaagttgt gcggcaattg tacgcataga acgggtgagt tcgaggccca
    18781 gtgcgtcttt gtatttgagg taggtgtcta caattgggtt cactgagatg gcagtttggc
    18841 tttcggctaa gctaataagc tgttgtcgct cttgtttata gaggcgcacc gttgttactg
    18901 ctgattgagc aaataaagcc accatcaaaa gaatagtaga catcacggct aacttaagac
    18961 gtatacttcg tagccacttc atgaaaagtc ctccttcaag ctctcgattt agtgcgcttg
    19021 cttcagcaag tgacgaattt tatgtaatag ttgttctggt tccgtttcga ctttctcaat
    19081 atattcaacg gcgccagctt ttaagctgtt atcgcgttcg agttcattga ctaaagcggt
    19141 cataaccatg acgggaatat ctttgagcgt atcatctttt ttaagggtct ttagcatggt
    19201 aaggccgtcc atctcgggca tcatgagatc gagtaggatg aggtcaggtt tatgttgcgc
    19261 cgcttttttt aagccttctt caccatttgt agcggtggta atagttaggc cggcttcttg
    19321 taaaaaatcc cgataggatg taataagtcg ttcattatca tcaatgatga gaatagtatg
    19381 aggggaagta tgtgtcatat cgtctcactt tctcctaata acgtaacacc tttttttaag
    19441 ggtaacaagc gtatacggta agccgttgcg gtttgggtaa ccagagtgtt acgctaaaaa
    19501 ggatgaaagt accgcattta gcgttatcaa gcagtgatcg aactgaacat gtgttgggcc
    19561 gacgactccg ttacattctt ttaggcgaaa tcatcctgat tttcgttctt tttacattgc
    19621 tccttactgt cgtagtagcg atcctctctc gtcagaatga agtgaaccaa atgagtatct
    19681 ctcgtttttc gatggcctta gaagttcaag gtgtcgagca gctactttta aaacatatga
    19741 attatgaaca gtcttaccgt ttaaatagta agcaaatggt cttagacgaa ttgctcgcaa
    19801 atcatatcgc tttcgatcag cggattaccg cattgtccca aaaattacaa acgctaggtc
    19861 cggacgaggc ctcattttta ccagaagaac aaaagctttc ccgtcggctc gctgcatcgc
    19921 accatgaaat aatattaata ctcgaaaaaa tggttggtaa gtcgctcacg ggtagtgagc
    19981 ataatcattc cggacttgat tcgtcttcaa ttacgattcc tttgacccct gctggtatgg
    20041 aagcgcgtat ttcgcggctc gaagaacaat cattcgccga cgcgggcatg tggcaacaat
    20101 ttgttcaggc agacatcgat cgcttgtcct atcagtcgga agtatatcag cggctccggc
    20161 ttactagttt atttggtttg gcatgtctca tgatactcgt attggtactg gtaaattata
    20221 cgtatgtgtt gccctctttt aaccatatcc tccgtcatgt ggtgcgtcag aacaaacaat
    20281 tgcgcgaagt cgattcgctt aaaaccgaat ttctttcggt ctctagtcat caattacgca
    20341 ctcctctctc ggaattaaaa tgggccctta ctcttcttaa tcggcaccag aacaaaggtg
    20401 accttaccaa gcagcatcac tttgtgcagc aatcacttca tagtgttgat acgatgtctc
    20461 aaatcgtatc agcccttttg aatgtgtcgc gcattgaaca gaatcgcctt caatttaatc
    20521 ccgttgttac cgacatggct cccgtcatga gaacggttat aaatcgcgcc aagcgagcag
    20581 ccaaagccaa agacgtcaca cttacgtttg tgagcccatc tggggggatg aaagcccatg
    20641 tcgatgttct gctggtaaaa cagatactcc aaaatctaat tgataacgca attcaatata
    20701 atcgtccgca tggaaaagtc gcggtgatcg ttaagaagca gcgagcaagt tggttaatta
    20761 gtatttcaga taccggttct ggtatctcgc ggaaagattt aaagcatctc tttacgaaat
    20821 tttatcgtgg cgacaacgca cgtagaattc ggcctgacgg aagcggtctt ggtctctact
    20881 ttgtaaaaaa gattgttgag aaacaccgtg gtaaaatcac cgtatcaagc atagaaggac
    20941 acggaacgat attttctata acactaccag gagttcgggg ctagagaacc ggtaggcagg
    21001 gagtggatac taaaaagacc caccgtttgg tgggtctttt tagtatcatg tgatattcgc
    21061 ttagcgagtg gcacagatag tcttaacgac agtgtcggta agagcattag gcgcgcaaac
    21121 agtgataacg tttgctgcac ttcgagcaat cgtgtatttc gtgtcttgtg cagttgttgc
    21181 agtctgctgg ggatcccgag gaagagccga gatataagca acggggctaa gtactgcgtt
    21241 aagggcgatc ccgccggtag ggcaagcagt gactttcgtg ctgtcacaaa tctcttccgc
    21301 agcagcgtca gcagttaggc cggcaacggt agctgggaaa gatccattgt tgtcagcagc
    21361 gtactggtgg acggcgttca agatagcgag agtgtcgctt tggcgaacag cattacgagc
    21421 ctgtgagaac tgacgggctg ggttaaccgc aacgatggtg atggcgccaa ggattgcgat
    21481 aataccaata acgacgataa gttcaattaa ggtaaaacct tttagtgttt tatgcataac
    21541 cactcctttc aataatgagc gataaaaaat gtcacgtatc ccatcgaccg agagaacacg
    21601 gtagcataca ttgctatcca ctttacaata acatagccgt tcggcaatgc aagcgaaagt
    21661 ataataggta atgagagcaa agtcatcgta tgtttaccat acaaaaaacc gaggtgtatt
    21721 cctcggtttt tttgtatggc ggagagagag ggattcgaac cctcgagggc ttgcgcccaa
    21781 cgcgctttcc aagcgcgcgc cataggccac taggcgacct ctccaaaaat attcttctgc
    21841 tagtataccg attttccctg aaagactcaa tgcgggttcc cttgtcaact ctgttataat
    21901 aatctcgata tgactgatac agtcgttgaa gatattaaat ccaggcttga catcgtggac
    21961 attattggta actacgtgac actccgtcaa tcgggcacca attttaaggg tctgtgccct
    22021 tttcacaatg aaaagtcacc ttctttcatg gctaaccgag aacgtcaaat ttttcggtgt
    22081 tttggctgtg gtgaggcagg tgatattttt gcttttgtga tgaaacaaga aaatattgat
    22141 tttccgaatg cgcttgctct cttagcctca aaggctgggg tggaattacc ggaaaagttt
    22201 acaaaagggc gtaaatctgg cgtagaaaaa tcagctttgt atacggtcaa tgatttagca
    22261 acgaagtttt ttaatcatat ccttgtgcag catccggcag gaaaagaagc cttggactac
    22321 cttcataagc gcggggtgaa aaatgaactg attgagagat ttcaaattgg ctatgcgcct
    22381 tcgtctaacc tggctattca acaattattt aaaaaacacg actttactac tgatgccgtg
    22441 aggttggctg gttctcccga acgttatcgt gatcggatta tgtttccatt ccgtgatgtg
    22501 attggccagg tggtggggta ttcggggcga gctttgcatg atcaggtacc taaatatctt
    22561 aatactgccg aaacggattt atttcgtaaa aatcgatatg tttatgggct gttcgaggct
    22621 aaaaaagcta ttgcagaagc gaaacgtgtc atgctcgttg agggacagct tgacttggta
    22681 ttagcgcacc aggctgggac agcgtatgcg gtggcaactt caggtacagc cttaaccgat
    22741 gaccatctta ctattctccg tcgatatagt gataccttac tattggcctt tgatgctgat
    22801 agcgctggcc aaaaagcaac agaacgggct attagtttag cgttgagtca tgggtttgaa
    22861 attaatgtgg ttgttttacc tgctggtaaa gatccagggg agattattgc tacggatcgc
    22921 tctgcctggg atagggcagt gaataaacca ttaagtgcaa tagaatggct ttttggttac
    22981 tacttccccg acataagtaa acgtccttcg gcaacagagc ggaaaaaaat ctatgacgct
    23041 ctttttccat acgttgataa attagccgac gtggttactc gctcctatgc gttgcagcgg
    23101 ctggcttttt ctatgggcgt cacgcaagag aaggcgctag tagacgcctt tgaagaatgg
    23161 aaagaaacgc cgccctcttc agagccttca cggaggattt cttctcaacc agaacaagaa
    23221 aaagtaggag aacccaacca gcctgcgttc gtaagtgagg aagctcgtcg ggaaaaatcg
    23281 attgtcggta tagtgcttct tcatcctgac ttgcttattc ataagcagct tcatctcgag
    23341 gaaaaagatt ttacgaatga ggcgctccac cacctataca caacgatttt aagttggtat
    23401 aataagaaca caaatcaatc aaacctctct caacttatat ccagcgttga aacaacgctt
    23461 tccgctgcag cgcggcaatc gctgcaaaag ctcatctttg atacccaaac agaactcgcc
    23521 gaacttactc gtgaacaagt cgtcggtgaa tatacattct tagttaaatc agttcgccaa
    23581 aaacaacgcg atgagcgtat gcagcactat gccgatgaaa ttcgagcagc tgaagcggcg
    23641 aacgatagac tacgtgtcgt gcagcttatg caagaaatgc aggctgcgct taaggaaaag
    23701 gagttgcatg ccaaagaaaa tccgtaagct ggtaga
//
LOCUS       DASWYB010000067        14093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_663303, whole genome shotgun sequence.
ACCESSION   DASWYB010000067 DASWYB010000000
VERSION     DASWYB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14093
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663303"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..569)
                     /locus_tag="VGE59_03870"
     CDS             complement(54..569)
                     /locus_tag="VGE59_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074806.1"
                     /translation="MATTIIGKIILRTDVPARGRTADGKERVFLNFIPDANFGLQRMW
                     GSYMFWRTAPYEGAVSRVHVWKHFDSMRSNQWKEVLPRSGDLMFPQQLQWGLEQVAHD
                     LNCDIHIKFDEEFLDPDKPEARAIRCCIHLFGLTEVRNDSGFLEAVATRIDREARRWS
                     AESSDWSIEDI"
     gene            complement(1020..1094)
                     /locus_tag="VGE59_03875"
     tRNA            complement(1020..1094)
                     /locus_tag="VGE59_03875"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1060..1062),aa:Lys,seq:ttt)
     gene            complement(1181..1714)
                     /locus_tag="VGE59_03880"
     CDS             complement(1181..1714)
                     /locus_tag="VGE59_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074807.1"
                     /translation="MVLLQSAPSWDLVITLVFVIGVSYGFIMLRDRILVTLLSLYAGM
                     MIATTLAEPIHKFFNGDIALLNKIWVESSASPFLIKLFLFGGTVLLLGAKSGLGGRRS
                     NFSFMELATYSFLNVCIALTAVFSFMEPDVLQGYINASKLVATVVQHQNLWLIAPLVV
                     MLVLGGSAGGRRHPDDY"
     gene            complement(2010..2630)
                     /locus_tag="VGE59_03885"
     CDS             complement(2010..2630)
                     /locus_tag="VGE59_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY1074808.1"
                     /translation="MKDYYDILGIKKGASQDEIKRAYRKMAHKYHPDKATAEQKAEYE
                     TKFKELNEAYQVLSDEQKRARYDQFGHAGVNGQGNPFGGNGGYNPFGGQGGFSGGGFG
                     FGGFETIIEDLMGSAFANISAEVHISLTQAVLGDTITLRTNTGESIELKIPAGTADGQ
                     TFVFRGKGQQHRRGRGDLHITVRVELPRRLTKEQKKLFEQLRECGL"
     gene            complement(2840..4744)
                     /gene="dnaK"
                     /locus_tag="VGE59_03890"
     CDS             complement(2840..4744)
                     /gene="dnaK"
                     /locus_tag="VGE59_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY1074809.1"
                     /translation="MGKIIGIDLGTTNSVASVMEGGKAKVIPTAEGGNITPSIVAENK
                     AGERLVGVLAKRQAITNPENTLYSVKRLIGRKFEDDEVQRDLKALPYKIVKDGDKVKV
                     AMGAKEYSPEEISAFILQKIKSDAEAYLGEKVTDAVITVPAYFNDAQRQATKDAGAIA
                     GLNVSRIINEPTAAALAYGVDKTDKKDRKVAVYDFGGGTFDISILDIGVHEGDSSIEV
                     LSTNGDTHLGGDDIDRRVMDWIISEFKREEGIDLSSDKIALQRIKEAAEKAKIELSQS
                     QQTEINLPFITADATGPKHLVKSMTRAKLEELTRDLIERTKPICEQALRDAKVSTSDI
                     DEVILVGGMTRMPLIQEVVQAAFGKEPSRGVNPDEVVAVGAAIQGGVLGGEVKDILLL
                     DVTPLTLGLEVEGGLLAPLIERNTTIPTSKTQVFSTAADNQTSVEIHVLQGERPMAAD
                     NKTLGRFMLDGIPPAPRGVPQVEVTFDLDANGILKVTAKDKATGKAQDITITASGTLS
                     KEEIEKMRADAEAHKAEDEKKKDLVETRNTAERLINLSEKTLKDAGDKAKPEDKREVE
                     DAITAVKSVMNSDNLEEIKAKTEKLSEVIQKVGSAMYEAAKDEPATTEEKSEEATEDQ
                     KEESSEEEKK"
     gene            complement(4868..5356)
                     /locus_tag="VGE59_03895"
     CDS             complement(4868..5356)
                     /locus_tag="VGE59_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY1074810.1"
                     /translation="MNKSDTSQLAEEQTDDRTTELEAQLKRVAADFDNFRRRTEEEKK
                     DLYSLAQAATLLEIAPVMDNFRRATSHLPEHLQNDNWVTGVLYIEKQLEQIFEQFGVT
                     KIKTVGETFDPRLHEALSTEPSDSVPANAIIAEFESGYMLKTTVLKPAKVKVSSGKNE
                     AE"
     gene            5414..6847
                     /locus_tag="VGE59_03900"
     CDS             5414..6847
                     /locus_tag="VGE59_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY1074811.1"
                     /translation="MTIIARMPKRFRFVCLLCGYLIGLALGSWFAWGISEYLLYGIFA
                     FLGLSFLFRNYSLSLLFLTLACAVCGYSWSQWHLQRLTLQEPIEYTGVVEVVSVRFSQ
                     VPRQRVVVRMLDGAYAHAAIQSYVYDWAYDPGTKILIHTSIEPSDFRPNLGAGILGQA
                     TNLTIREVVPSQDRLVAFRNQVQERMGSSIPEPYASLAVGLLTGQNDSFDLHFKEDLQ
                     RTGTTHLVAVSGYNLTIVALLLQRLGRRKNRYIGYGLALSSILFYVILAGANPSILRG
                     AMVAFLSLSAGVAGRITHRTTLVLMSAVLLSFLTPLGMLYSLSWQLSFLAFVGILFVS
                     PVVTPYLENKFGFLGATMGETLSAELLVLPLVLKQFGVLSIVSPLVNTVVLGITPLAM
                     ALSALQATISLISLEMGRLFAWVTYPILWSIVTPIQWASKLPFASYQISSFSWLYFVV
                     SYCLVGLLFGVLSVMHGRKTDDETQLT"
     gene            6825..7709
                     /locus_tag="VGE59_03905"
     CDS             6825..7709
                     /locus_tag="VGE59_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1074812.1"
                     /translation="MTKPNLLKLALMFVFFGLIALGSAGFLPQASIEEPGGLKVAFMD
                     VGQGDAILLRTPHRHYVLIDGGPDGTILSRLGEEMRFNEHTIDLVVVSHNHSDHIAGI
                     NRLIDRYDVQKMWISGAIHTTNEYLKLLDNIKGNSIPTEVVFKGKQVEIDGVKLQVLH
                     PVESTESVRPEDQHDATIVIKATYGEKSLLLTGDIDEGHEQNIIKSLGDAAGTVLNSD
                     VLKVPHHGSKSGLALNFLNFVDPKYAVIQVGAKNSFGHPAASILQKLRDKGVRVFRND
                     QDGTVRFYSDGKDIIVNP"
     gene            7759..8496
                     /locus_tag="VGE59_03910"
     CDS             7759..8496
                     /locus_tag="VGE59_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1074813.1"
                     /translation="MSGHSKWATIKRQKGAADAKRSVTFTKLAAAITVAARDGGGDLG
                     SNFKLRLAVDKAREANMPKDNIDRAIKRGTGELGGAAFEHIVYEAYAPGGAALVIDVN
                     TDNRNRAVANIKSILNKHNGKLAESGAVAYLFQPRGVMTVAGGDAEAMEMAIIESGAD
                     DYALSDDSAVVYTDPKEVMAVAKALETAGLTASDVEQTMEPVSTIMISDEKTAKTLVA
                     ITELLEDLDDVSSVYSNFDIDESFNIG"
     gene            8551..9018
                     /gene="ruvC"
                     /locus_tag="VGE59_03915"
     CDS             8551..9018
                     /gene="ruvC"
                     /locus_tag="VGE59_03915"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY1074814.1"
                     /translation="MDPGTGRCGFAAVRFTNHQPTILTYGCFEYPSKMPLPERYVELE
                     RDLSTILDEHKPSVLAIETLFFNRNVTTAMQVSEARGVITLCGAKRGISIQPCTPMQV
                     KVAVTGYGKAEKKQVQQMIKLQLGLKELHKLDDAVDALAIAIAGYHLYTSRIN"
     gene            9073..9636
                     /gene="ruvA"
                     /locus_tag="VGE59_03920"
     CDS             9073..9636
                     /gene="ruvA"
                     /locus_tag="VGE59_03920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY1074815.1"
                     /translation="MIRYLHGKVIDKVRNHVVIDVHGVGYGVMVTPQTMTQVETGQEM
                     SVHISESIREDGYDLYGFLSSTERDMFELLRKVSGVGPKVAMALTGFYSPADLQSIIH
                     SNDVAKLSLVPGVGKKVSEKIIVELKDKTKDAGFSSASPTDADTVAALQSLGYSPAEI
                     AKALPKVPANLATTSERITWVLRHLAD"
     gene            9636..11678
                     /locus_tag="VGE59_03925"
     CDS             9636..11678
                     /locus_tag="VGE59_03925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY1074816.1"
                     /translation="MVHLGQSIINIKGMGPKTAEAFESHGIRTVEDLLYFFPRAWMNA
                     STVVPIAELKFGMSALIEVQVAAMKEGMSQRTRIPYLRATVADTTGYLDVMWFHPKYL
                     RGKVRVGTKLLLYGQMQGFRQQDRIMMSPKILKEPCIQAVYPTIGGVASFKTRPIMEQ
                     LKDVVETIPDYLPESIRMEHSLLPLSQTIKSMHFPADITHINLARLRLGFDELLMLMV
                     PSLVAKSERDQEKTESLTLDNGKVKTWCDSLPFALTEDQQTVIAEILQDLGKTKPMNR
                     LLQGDVGTGKTVVGLAAALQAVLAGKQVAWLAPTELLAKQHFETAKKLFAPHSVGLWT
                     RTHHFAYVNGEEQKSTVDEVTGLSLVIGTHALLSEKVVLRDLGLLIIDEQHRFGVKQR
                     AQLRLFGGHPVHLLSMTATPIPRTMALLLYGDLSLSVIKQKPAGRKEIVTRVVSDTSR
                     DKAYQFIEDQIAKGGQVYVVCPLIEPAKDDEANMFTQLFEPVEEKKAVTTWATELQKQ
                     FPNRKVLILHGKMKAAEKDSVMQSFRAGETDILVSTTVIEVGVDVPNANIMIIEGAER
                     FGLAQLHQLRGRVGRGERQSFCLLFPSSSAAIDNDRLKIMEQTNDGFVLAEKDLELRG
                     PGELTGLSQSGIPPLRFASLQNTEQVQKVQSIAAELLANPAFKQSLERFWRQHHPE"
     gene            11701..12123
                     /locus_tag="VGE59_03930"
     CDS             11701..12123
                     /locus_tag="VGE59_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY1074817.1"
                     /translation="MDWRQYFHTGARQVIGMFLIVFALAGAWLYREELRVLEMSRTSV
                     QIVTSGVSASLTSLTSPSAEIVLQITPLPTVTVNNTIKLSTATQGELETLPGIGPAKA
                     KAILDYRESEGFKTVDDLEKVKGIGPKTLEKLRPFVEL"
     gene            12140..12730
                     /locus_tag="VGE59_03935"
     CDS             12140..12730
                     /locus_tag="VGE59_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1074818.1"
                     /translation="MTFDYRKESHPWGIEPLDELKQFATQLKSGTVLDLGGGDGRNSL
                     YLAHLGFSVTNVDRSPAAIASFLERAEAESMRDLCTGIVSEVGSFSFQSYDMILAINI
                     IHFLSPNLFPELFHQIKESTRPGGVVIIADYLDGGPFRNPEYDQYWLQSGELKSLYRD
                     WHILEYKEVMRIPRKINSDGAPMENMWALLVARKPE"
     gene            complement(12714..13295)
                     /locus_tag="VGE59_03940"
     CDS             complement(12714..13295)
                     /locus_tag="VGE59_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074819.1"
                     /translation="MSQGHYYRTMHYLYHMVPKDLVGDILYPLNKLKLLDPELYDKKT
                     KKYQGREYQLELKVPPLNCLWNDVLHLSPIHPQEVVDTLKACGDTGPYTHRCYQIDPY
                     FLDKELTTVFLYSKPKERRPRFEVPADDFIDYDPNKIEQYAHYSQLTKDYFCRMYADG
                     KYPLLYHFVPHILYKGTINVSNLPIIEASIPAS"
     gene            complement(13346..13421)
                     /locus_tag="VGE59_03945"
     tRNA            complement(13346..13421)
                     /locus_tag="VGE59_03945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13386..13388),aa:Val,seq:cac)
     gene            complement(13478..13561)
                     /locus_tag="VGE59_03950"
     tRNA            complement(13478..13561)
                     /locus_tag="VGE59_03950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13526..13528),aa:Leu,seq:tag)
     gene            complement(13811..>14093)
                     /locus_tag="VGE59_03955"
     CDS             complement(13811..>14093)
                     /locus_tag="VGE59_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074820.1"
                     /translation="QAILSSGHQCVGGQCNLCRVVPAHIPSSRGRWVIDVDEETAQLT
                     EVGATRHEASPARPAAIAPPVAQTPRPVPTPPVRVVTEEEPGSHWTDRI"
BASE COUNT         3501 a         3056 c         3518 g         4018 t
ORIGIN      
        1 gaaagccccc gacacgaggc cgaaggcttt cgtgtacggg ggcggggtca gggctagatg
       61 tcctcgatgg accagtccga agattcggct gaccagcggc gggcctcgcg atcgatccgc
      121 gttgccactg cttccaagaa accgctgtcg tttcttactt cggtgagacc gaagagatgg
      181 atgcagcagc gtatagcccg tgcttccggc ttgtccggat ccagaaactc ctcatcgaac
      241 ttaatatgga tatcgcagtt gaggtcatgg gcgacctgtt cgagtcccca ctgcagttgc
      301 tggggaaaca tcaggtcgcc acttcggggg agtacttctt tccactgatt actcctcatg
      361 gagtcgaagt gtttccatac atgtacccgg ctaacggctc cttcgtaggg agccgttctc
      421 cagaacatgt aggagcccca catcctctgg agcccgaagt tggcatcggg tataaaattc
      481 aggaatactc gctcttttcc gtccgcagtc ctaccgcggg caggaacgtc ggtccggagg
      541 atgatcttgc cgatgatggt cgtcgccatt ttaagatcct catttcaccc tctgcacgag
      601 aggcgtgtag cctatgttta cgtcagacct ggctagaggt ccgtggaagt gaacgcgaga
      661 gtgcgttctg tccagcaagc tacttagggc taagcagtat tgctatacag aagtccatag
      721 ccttctcagc tgttacaaag catggcgaga atataccata aaaatgggtt tttgtcaagc
      781 agtcacgtaa ttaataataa aactctaacc tattgtaaac gaaaaagccc accttgcggt
      841 ggacttcttc atggagggcc tgatggtgta cgtcgccgac atggaaatta tatatgtcgg
      901 ctttggtata attcccgccg ctgcgctctg ggctttcagg aattatcgaa cccgcataga
      961 ttgtcttacc catccaaact ctccaaccaa aaaaccacct tttcgggtgg cttcttggtt
     1021 ggagggcctg atgggattcg aacccatgac acgaggctta aaaggcccct gctctacccc
     1081 tgagctacag gcccacgatt gattcgtgga acagaactat tataccgaaa atacccgagg
     1141 aaggcaataa tctgatggct gtgttttgca aattgaaaaa ctaatagtcg tcggggtgac
     1201 ggcgtccacc cgcagaccca cctagaacga gcatcacaac cagaggagca ataagccaaa
     1261 gattctgatg ttgcacaacc gtagcaacaa gttttgaggc gttgatgtaa ccttgtaaaa
     1321 catcgggttc cataaagcta aatacagccg tgagggcgat gcacacattc aaaaagctat
     1381 aagtagcgag ctccatgaag ctaaagttgc tccgtctccc gccaaggcca ctttttgctc
     1441 cgagtagtaa tacggttcct ccaaagagga atagtttaat caaaaaagga gaagcagaac
     1501 tttcaaccca aattttattt aagagggcaa tgtcgccatt aaagaacttg tggatcggtt
     1561 ctgcgagagt tgtggcgatc atcatgccgg catatagaga taaaagcgtc accaaaatgc
     1621 ggtcacgcag cataataaag ccgtaactta caccaatcac gaatactagg gtaataacca
     1681 agtcccacga tggggcggat tgcagtaaaa ccatttccct ccgtcaagca ggttagcgac
     1741 tgcttataaa cagcatagag aattggcgcc agttgtacaa gatcgttagg tggtgtggac
     1801 gttagtcctg ttctcaaaaa agctttaaac gaatatctgg gccaggttga aacgtaggta
     1861 ttgacaaaaa gaccgtttta tagtatattt ctaatcaatg acacaaagga cgtactggca
     1921 acaagaggcc tgactgtcag actgggcatg cccagtgttt cctcctcgaa tgccagctaa
     1981 cccgccccct ccatccggag ggggctttac tataatccac actctctcag ttgctcgaaa
     2041 agcttttttt gttctttggt tagtcgtcga ggaagttcga ctcgtacggt aatatgcaaa
     2101 tcgcctcggc cacggcggtg ctgttgacct ttgccccgaa aaacaaaggt ttgaccatcg
     2161 gccgtaccgg ctggaatttt aagttcaatt gattcgcccg tattagtgcg aagcgtgatg
     2221 gtgtcgccta aaacagcttg ggttaacgaa atatgaactt cggcgcttat gttggcgaaa
     2281 gcagagccca tgagatcttc gataattgtt tcaaatccac caaacccaaa ccctccgcca
     2341 ctaaatccac cttggccacc aaatggatta tagccaccgt tcccaccaaa aggattccct
     2401 tggccgttaa cgccagcatg gccgaactgg tcgtagcgag cgcgcttttg ttcgtcagaa
     2461 agtacttggt atgcttcgtt gagttcttta aactttgttt cgtactcagc tttttgttcg
     2521 gccgtcgctt tgtccggatg gtatttatga gccatcttgc gataggcacg tttaatctcg
     2581 tcttgtgacg cgcctttttt gattccaaga atgtcgtagt aatctttcat agttgtatat
     2641 agtatgtttc ttaacagtgt tatctcttcg tgaatgcttc tgttgccctg tgggcggatc
     2701 tctagactgc agccgggtat gaaacattgg ctagccagca atgagttacg agcgcgcttg
     2761 gtacgtccga atggccttcg ggagcggaat gcgaaacctg gcaaggggcc ggacatgtgt
     2821 ccggcccact tattttacct tattttttct cttcttcgct tgattcttct ttttggtctt
     2881 ccgttgcttc ttcggatttc tcttcggttg tagctggttc gtcttttgca gcttcgtaca
     2941 tggcgctacc aactttttga atcacttccg agagtttctc tgtcttcgct ttaatctctt
     3001 cgaggttgtc gctgttcatc acgctcttaa cggcggtaat agcatcctcg acttcacgtt
     3061 tgtcttcagg cttggctttg tcaccagcat cttttagtgt cttttctgac agattaatca
     3121 gtcgttcagc ggtattacga gtttcgacga gatctttttt cttttcatct tcggccttgt
     3181 gggcttcggc gtcagcgcgc atcttttcga tttcttcttt cgaaagtgtg cctgaagcag
     3241 taattgtaat atcttgagct ttaccggtag ccttatcttt agcggttact ttgaggatac
     3301 cgttggcatc gaggtcaaag gtgacttcga cttgtggaac gccgcgaggg gccggtggga
     3361 taccgtcgag cataaagcgg ccaagggttt tattatcggc ggccatggga cgttctccct
     3421 ggagtacatg gatctcgacg ctcgtttgat tatcggcagc ggttgagaat acctgagttt
     3481 tgctcgttgg aatagtggta ttgcgctcaa taaggggagc taggagtcca ccttctactt
     3541 ctaggccgag cgtaagcgga gtgacgtcga gaagtaagat gtctttgact tctccgccga
     3601 gtacgccacc ttggattgct gcacctaccg ctacgacttc gtcagggtta acgccgcgag
     3661 aaggttcctt gccaaatgca gcttgaacta cttcttggat gagtggcata cgagtcatgc
     3721 ctccaacaag aataacttca tcgatgtcgc tggtcgaaac tttggcgtca cggagagctt
     3781 gttcgcaaat aggcttggtg cgttcgatta aatcccgagt taattcttcg agcttggctc
     3841 gtgtcatgct cttaactaag tgtttggggc cagtagcatc agccgtaata aacggcaggt
     3901 taatttcggt ctgctggctc tgggagagtt cgatcttagc tttttcagcg gcttctttaa
     3961 ttcgctgaag ggcaatctta tcgcttgaaa gatcaatccc ttcctcccgt ttgaattcgc
     4021 taatgatcca gtccatcaca cggcggtcga tatcatctcc tccgaggtga gtatcgccgt
     4081 tggttgagag gacttcaata ctggaatcgc cttcgtgaac accaatgtcg aggatgctga
     4141 tatcgaaggt gccgccacca aagtcatata ccgcgacttt acggtctttt ttatcggttt
     4201 tgtcgacacc ataagcaagt gccgcagcag taggttcgtt aataatgcgg ctcacgttga
     4261 ggccggcaat ggcgcctgca tctttggtgg cctggcgctg agcgtcgttg aagtaggctg
     4321 gtacggtaat aacggcgtca gttacttttt caccaagata cgcttcggca tccgatttaa
     4381 ttttttggag aataaaagcc gaaatttctt cggggctgta ctcttttgcg cccattgcaa
     4441 ctttaacttt gtcgccatct ttaacaatct tataaggcaa agcttttaag tcgcgttgga
     4501 cttcgtcatc ttcaaatttg cgtccaataa gacgttttac cgagtaaagc gtattctcgg
     4561 gattggtaat tgcctgacgc ttggccagga ctccgactaa gcgttcgcca gctttatttt
     4621 cggcaacaat tgatggcgta atattgccac cttcggcagt aggaatgact ttggcctttc
     4681 ctccttccat tacggaagca acagagttgg ttgtacctaa gtcgatacca ataattttac
     4741 ccataaaagc tcctttcact atttcctaat aagctgatag taaagtattc aagaattatt
     4801 gtaaagtaaa ctttacatac gtgtcaatag caggtttgaa aaagctcctc agagaggagc
     4861 tcgccgctta ttctgcttcg ttttttcctg aactaacttt tactttagct ggtttgagaa
     4921 cggtagtttt aagcatgtag ccgctctcaa attctgcaat aatagcgtta gccggtactg
     4981 aatcactcgg ttctgtactt aacgcttcgt ggagccgtgg atcaaaggtt tctccgacgg
     5041 tttttatttt cgttacgcca aattgttcaa aaatttgttc aagctgtttt tcgatgtaga
     5101 gcacacccgt tacccagttg tcgttttgaa ggtgttcagg caaatggctc gtggcgcggc
     5161 ggaagttatc cattacgggc gcaatttcaa gaagcgttgc tgcttgcgca aggctataaa
     5221 ggtctttttt ctcttcttcg gtccgtctgc ggaaattatc aaaatccgct gcaactcgct
     5281 taagttgggc ttcgagctct gttgtgcggt cgtcggtttg ttcttcagct aattgagaag
     5341 tgtctgactt gttcatgact ctctttatac cgcactctat cccacgtggc aagaatggta
     5401 tagagtttaa gtaatgacta ttatcgcccg tatgccaaaa cggtttcggt tcgtgtgtct
     5461 tctatgtggc taccttatcg ggctggcgct tggttcatgg tttgcatggg gtataagtga
     5521 gtacctactg tatggtattt ttgccttctt agggctctcc tttctttttc gaaactattc
     5581 actgagtctt ctttttttaa ctttggcttg cgcggtatgt ggttattctt ggtctcaatg
     5641 gcatcttcaa cgtttaaccc ttcaagaacc aatcgagtat acgggagttg tagaggttgt
     5701 ttcggttcgg ttttcgcagg ttcctcgtca acgggtggtg gtacgaatgc tcgatggggc
     5761 atatgctcat gcggctatcc aatcgtatgt gtatgattgg gcatacgacc ccggtacaaa
     5821 aattttgata cacacttcga ttgagccaag tgactttcga cccaatcttg gtgcgggtat
     5881 tttgggccaa gcgacgaacc taaccataag agaggttgtg ccaagtcagg atcggctcgt
     5941 agcgttccgt aatcaagtac aagagcggat ggggtccagt atccctgagc catatgcgag
     6001 cttagcagtt gggcttctta cagggcaaaa cgattcgttc gatcttcatt tcaaagaaga
     6061 tttacagcgg actggaacga ctcacctagt agcggtttct ggttataacc ttacgattgt
     6121 ggcgctgttg cttcagcgct tgggacgtcg aaaaaacaga tacattgggt atggcctcgc
     6181 gctttcgagt attctctttt acgttatttt ggccggtgcg aacccttcaa ttttacgagg
     6241 ggcaatggta gcgtttttat cgctctcggc aggggttgct gggagaatca ctcaccgcac
     6301 caccctcgtc ttaatgagtg cggttttact ttctttcttg actccgctag gaatgcttta
     6361 cagcttatcc tggcagttgt cgttcttagc ttttgttggg attttgtttg ttagccctgt
     6421 cgtaacccca tacttggaaa ataaatttgg atttcttggt gcgacaatgg gcgagacgct
     6481 gagtgccgaa ttgctcgtat tgccacttgt ccttaaacag tttggtgtgt tgtcgattgt
     6541 ctctcccctt gtgaataccg ttgtacttgg cataacgccg cttgctatgg cgcttagtgc
     6601 gctgcaggca actatttcgc tgatatcgct ggagatgggg cgtcttttcg cttgggtgac
     6661 gtatcccatt ctctggtcga ttgtgacgcc tattcagtgg gccagtaaac taccctttgc
     6721 gtcctatcaa atttcctctt tctcttggct ttactttgtg gtgagctatt gtttggtagg
     6781 attgctcttt ggagttctct cagtgatgca tggaagaaaa acagatgacg aaacccaact
     6841 tacttaaact tgctttgatg ttcgtatttt tcggcctgat tgctctggga tcagccggtt
     6901 ttctgccgca agccagtatt gaagagccgg gtggtctaaa agtcgctttt atggatgttg
     6961 gccaaggaga tgcaatttta ttacggacgc cccatcggca ttacgtactc attgatggtg
     7021 gccctgatgg gactattttg agtcgccttg gcgaagaaat gcgttttaat gaacatacaa
     7081 ttgatttggt tgttgtcagt cataatcatt cagaccatat tgccggtatt aaccgattga
     7141 ttgatcgtta tgatgtgcag aaaatgtgga taagtggggc tattcatacg acgaatgaat
     7201 accttaaatt gctcgataat attaagggta atagtatccc aaccgaagta gtctttaagg
     7261 ggaagcaagt cgaaattgac ggagtgaagc tccaagtgct ccatcctgtt gaaagtacgg
     7321 aatcggttcg acccgaagac caacacgatg cgacgatcgt cattaaagcc acttacggcg
     7381 agaaaagtct tttactgacc ggagatattg atgaagggca tgagcaaaat attatcaaat
     7441 ctttaggaga tgcagcagga actgtattaa attcagatgt tttaaaagtt ccgcatcatg
     7501 ggtcaaagag cgggttggct ttaaattttc taaactttgt tgacccaaag tatgcggtta
     7561 ttcaggtagg agcaaagaat tcctttggtc acccagcggc gagtattttg caaaaactta
     7621 gagacaaggg cgttcgagtt tttcgcaacg atcaagacgg aaccgtacga ttttatagcg
     7681 atggaaaaga tataattgtt aatccctaaa aggcgtgagg tcgaattttt aacgggttta
     7741 ggttataata ggttcattat gtcaggacac agtaaatggg cgacaatcaa gagacaaaag
     7801 ggcgctgcgg atgcaaaacg gagtgtcacg tttaccaaac ttgcggcagc tattacggtt
     7861 gcggcacgcg atggaggtgg tgacttaggt tctaatttta aactacggtt agctgtcgac
     7921 aaagcccgtg aagctaatat gcctaaagat aatatcgatc gggcgattaa gcgtggtact
     7981 ggtgagctgg gtggggcggc gtttgagcat attgtgtacg aagcttacgc cccaggggga
     8041 gcggcgttgg ttattgatgt aaacacggat aaccgcaatc gtgcggttgc taatataaaa
     8101 tcaattttaa ataagcataa tggcaagttg gcagagtccg gtgccgtggc atatctcttc
     8161 cagccccgtg gtgtgatgac ggtagcgggt ggggatgcgg aagctatgga gatggccatt
     8221 atcgaaagcg gcgcggatga ctacgctcta agcgatgata gtgccgtcgt atacactgac
     8281 ccgaaagaag ttatggcagt tgcgaaagct ttagagacag ctggacttac ggcttctgat
     8341 gtcgagcaga caatggagcc ggtttccacg attatgattt ctgatgaaaa aacagccaag
     8401 acgttagtag caatcaccga gttactcgaa gatctggatg atgtttcttc agtgtactca
     8461 aattttgaca ttgatgagtc tttcaacatt gggtaatccc tgctagtgta gcctcaggat
     8521 gttaaggagg ctcatatcac aattgttggc atagaccccg gtactggacg atgtgggttt
     8581 gcggctgttc ggtttaccaa tcatcagcct acaattttaa cgtacggttg ttttgagtac
     8641 cccagcaaaa tgcccctgcc tgagcggtac gttgagttgg agcgtgattt atcaacgatc
     8701 ttagatgagc acaagccatc agtgctagcg attgagacgc tcttttttaa tcgtaatgta
     8761 acgaccgcta tgcaagtatc ggaggcccga ggtgttatta cgttgtgtgg tgccaaacga
     8821 ggtatctcca tccagccatg cactcccatg caagtaaaag tcgccgtgac tggttatgga
     8881 aaggccgaaa aaaaacaggt gcagcagatg attaaattgc aacttgggct taaggagctt
     8941 cataagcttg atgatgcggt tgacgcatta gccattgcga ttgccggata tcacttatat
     9001 acctctcgca taaattgaca ggggctgtcc tccctggtct aatgagatta gctttacgag
     9061 ggagattttt ctatgattcg ttatttacat ggcaaagtaa tcgataaggt tcgtaatcat
     9121 gtcgtgattg atgttcatgg ggtggggtac ggtgtgatgg tgactcccca gactatgact
     9181 caagttgaga ctgggcaaga aatgtcggtt catatttctg aatcaatccg cgaggacggc
     9241 tatgatttgt acggtttttt atcatcgact gaacgggata tgtttgaact cctgcggaag
     9301 gtttcgggtg tagggcccaa agtcgcaatg gctctaacgg gtttttattc tccagctgac
     9361 ttgcagagca ttattcatag taacgatgtc gccaagttaa gtcttgtgcc cggagtgggc
     9421 aagaaagtct ctgaaaagat tattgttgag ctgaaagaca aaaccaaaga cgctggtttc
     9481 tcttcggctt ctccaactga tgctgacacg gttgctgcac ttcagtctct tggatatagc
     9541 cctgctgaaa ttgccaaagc attacctaag gtgcccgcta atttagccac aacgagtgaa
     9601 cgcattacct gggtattacg acacttggct gactaatggt acatctgggc caatcaatta
     9661 ttaatatcaa agggatgggg cctaagacag ctgaggcttt tgagtctcac ggtattcgca
     9721 cggttgaaga tttactctat ttttttcctc gtgcctggat gaacgcttcg acagtagtgc
     9781 ccattgctga gcttaaattc ggtatgagtg ccttaatcga agtccaagtt gcagcaatga
     9841 aagaagggat gagtcagcga acacgtattc cctatttacg ggcaacagtt gccgatacaa
     9901 ctgggtatct cgatgttatg tggtttcatc caaagtacct tcgtgggaaa gttcgagtcg
     9961 gtaccaagtt gttactgtat ggtcagatgc aaggttttcg ccagcaggac cgaattatga
    10021 tgtcgccaaa gattctcaaa gagccatgta tccaggccgt ctatcctacg attggcgggg
    10081 ttgcttcgtt taaaacccgg ccaattatgg aacaactcaa ggatgttgtc gaaacgatcc
    10141 cagattatct gcccgaatca atccgcatgg aacatagctt attaccgttg agtcagacga
    10201 taaaatcaat gcattttccg gcagatatta cgcatattaa tttagcgcgc ttacgtcttg
    10261 ggtttgatga acttttaatg ctcatggtcc cctcgttagt cgcaaaatcc gaacgagatc
    10321 aggaaaagac tgaatccctc acacttgata acggaaaggt gaagacctgg tgtgattctt
    10381 tgccatttgc tctcactgag gatcaacaga cagtcattgc tgagatattg caagatttag
    10441 gtaaaacaaa gccaatgaat cggttgctcc aaggggatgt gggaacgggc aaaacagtgg
    10501 taggtttagc ggctgcatta caagcagtac ttgcaggcaa gcaagtggcg tggctggcac
    10561 caaccgaact tcttgctaag cagcactttg aaaccgcgaa aaaacttttt gcacctcact
    10621 cagttggact gtggactcga acgcaccatt ttgcgtatgt aaatggggaa gagcaaaagt
    10681 cgacagtcga tgaagtgaca gggctttcgc tcgtaattgg tacccatgct cttttgagtg
    10741 aaaaagtagt tcttcgtgac ttaggattat tgattattga cgagcagcac cggtttgggg
    10801 tgaagcaaag ggcgcagttg cgtctatttg gaggacatcc cgttcactta ttaagtatga
    10861 cagcgacccc tattccccgt actatggcgc ttttgctcta cggtgatctt tctctttctg
    10921 ttattaaaca aaagccagca ggtcgaaaag aaatcgttac gcgggtggtg agtgatacaa
    10981 gccgcgataa agcctatcag tttatcgaag atcaaattgc caaaggcgga caagtctatg
    11041 tagtctgccc tttgattgag ccggccaaag atgatgaagc gaatatgttt acacagctat
    11101 tcgaaccggt agaagaaaag aaagcggtaa caacatgggc aacggaactg caaaagcaat
    11161 ttccgaatcg gaaagttttg attcttcacg gtaaaatgaa agctgccgag aaggatagtg
    11221 tcatgcaatc gtttcgagct ggagagacgg atatacttgt gagtacgacc gttattgaag
    11281 ttggggtcga cgtgccaaat gccaatatta tgattattga aggagcagaa cgatttggct
    11341 tagcgcagct ccatcagctc cgtggtcggg ttggtcgggg agagcgacag agcttttgtt
    11401 tgctttttcc ttcgtcgtct gcagcgatag ataacgatcg acttaaaatt atggaacaaa
    11461 ccaacgacgg ctttgttctt gccgaaaaag atcttgaatt gcgcggcccc ggtgaattaa
    11521 ccggtctttc tcagtcaggt attccgccgc ttcgatttgc ttcattgcaa aatacagaac
    11581 aagtccagaa ggttcagtcg attgctgccg agttattggc gaacccagct tttaagcaat
    11641 cgctcgaacg attctggcgt cagcatcatc cggagtaagg acttgtgtta tagtgaaagt
    11701 atggactgga gacagtattt ccatacgggc gctcgtcagg tgattggtat gtttttgatt
    11761 gttttcgctt tggcaggtgc atggctttat agggaagagc taagagtcct ggaaatgagc
    11821 cgaacgagtg ttcagatcgt aacctctgga gtctctgcct cacttacttc acttacttcc
    11881 ccttctgcgg aaatagtatt gcaaattact cccttaccaa cggtgactgt aaacaacaca
    11941 attaagcttt ctacggctac gcaaggagag ctggaaacat tgccgggaat tggccctgcc
    12001 aaggcaaagg ccattctcga ttaccgagag agtgaaggat ttaaaacggt agatgatctt
    12061 gaaaaagtca aaggtattgg cccaaagaca ctggaaaaat tacgtccctt cgtagaattg
    12121 taagttggag atttatagta tgacttttga ttacaggaaa gaatcccacc cttggggtat
    12181 cgaaccgcta gatgagctaa agcaatttgc tacccagctt aaatcaggaa cagtattaga
    12241 tttaggtggc ggggatggca gaaatagttt gtaccttgcg catctaggat tttcggttac
    12301 gaatgttgat cggtctcccg ctgcaattgc ctcgtttcta gaacgggccg aagcagaaag
    12361 tatgagagat ttatgtaccg gtattgtgtc ggaagtcgga agtttttcct ttcaatctta
    12421 cgatatgatt ctagccataa acattattca ttttctatcg ccaaacttat ttccagagtt
    12481 atttcatcaa ataaaagaga gcacaaggcc tggcggggta gtgattatcg ccgactatct
    12541 cgatggaggt ccgtttcgaa atccggaata tgaccagtat tggcttcagt ctggagagtt
    12601 aaagtcgtta taccgtgatt ggcatatcct tgagtataaa gaagtaatgc gtatccctcg
    12661 taaaataaat tcggatggag ctcctatgga aaatatgtgg gcgcttttgg tggctaggaa
    12721 gccggaatag aagcttcgat aatcggtagg tttgaaacgt taatcgtacc tttatagagg
    12781 atatgcggca caaaatggta gagcagggga tactttccgt cggcgtacat tcgacagaaa
    12841 taatctttgg taagttgtga gtaatgagca tactgctcaa ttttatttgg gtcatagtcg
    12901 atgaagtcgt cggccggaac ttcgaatctg ggtcttcttt ctttaggttt gctgtaaaga
    12961 aatactgtgg tgagttcttt atctaaaaaa taaggatcaa tttgataaca tcgatgcgtg
    13021 taagggcctg tatctccaca tgctttaagt gtgtcgacaa cttcttgggg atgaatagga
    13081 gagaggtgca gcacatcatt ccacaagcag ttgagagggg gaacttttag ctccagttgg
    13141 tattctcttc cttggtactt tttcgttttt ttatcgtaca gttctgggtc aaggagttta
    13201 agcttattta atgggtagag tatgtctcca actaaatctt tagggaccat atgataaagg
    13261 tagtgcatgg ttcgatagta gtggccttga ctcatatagt caatcttgac atagaaaaaa
    13321 accatcttgc gatgggcttt ttccgtggtg gagatgggag gatttgaacc tccgacttct
    13381 tcgatgtgaa cgaagcactc tggccactga gttacacctc cataaagatt aacctattat
    13441 aaaccagatg ggtactccgt tttgtgaagc attcatctgg tgccgaggag aggactcgaa
    13501 cctccacgcc ttgcggcgcc agctcctaag gctggtgcgt ctaccatttc gccacctcgg
    13561 catgtccaac ctgtaatgtc ttatacggtt gacacaaacg tattatatca gtaaaattta
    13621 taaatgtctg cttacggcag taagcatatt gtgactcgcg cgctagagta tttgactcct
    13681 tcatatggac gcatatggag accaaaagga aaccgctacg cccaaccggt gggacatagc
    13741 ggttttctgt tttaaaaaag aggagccgcg cagaggactg cgcggcgtga tctcagatag
    13801 agagggagga tcagattcga tccgtccagt gtgagcccgg ctcttcttct gtcacgactc
    13861 gcacgggggg tgtcggtact ggtcgcggag tttgcgccac gggtggcgct atggcagcgg
    13921 gtcttgcggg ggaagcttcg tgcctcgtag cacctacttc cgtcagttga gcggtttctt
    13981 cgtcgacgtc gattacccat ctcccccgag agctcgggat atgggccggt acgactcggc
    14041 acagattgca ttgtccccct acacactgat gccccgaact cagtattgcc tgt
//
LOCUS       DASWYB010000068         7415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_1004203, whole genome shotgun sequence.
ACCESSION   DASWYB010000068 DASWYB010000000
VERSION     DASWYB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004203"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..205
                     /locus_tag="VGE59_03960"
     tRNA            129..205
                     /locus_tag="VGE59_03960"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:163..165,aa:Pro,seq:cgg)
     gene            complement(384..914)
                     /locus_tag="VGE59_03965"
     CDS             complement(384..914)
                     /locus_tag="VGE59_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074821.1"
                     /translation="MPIELAVRMLCALAFACFAGYYGWYRQSERCRICYFDPKWRTVC
                     PEVRQQKFALLRSVLKMAVVVALLWYSADTSALTASPALPDYYRWVGVGLCLYGLFQI
                     LSVALVGPGLARFPFHGGFALWTFGLSLATSSWFVAVPLVLLLVLSLTRISWCQWYLP
                     GAFIVWPQAVRRMVMR"
     gene            1172..1942
                     /locus_tag="VGE59_03970"
     CDS             1172..1942
                     /locus_tag="VGE59_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074822.1"
                     /translation="MEAISEGVATEGRLLATKSRGKALERVCGICEIRNVVREAYCPI
                     CTAKIREVKTHLAQMKRPYTEGNIAQSIRRHWRVADAPQMVSIRIYARVWNTAIETII
                     QLCAQEGLATRGTREKKKEPHYLPVATVIFLDMLLEAGIAVQTKVRPRCTLCHHPAAG
                     NLPLCIRHLAVWEWMCEKIGKPTYMLPLIKLGQGEAISLGSLALLTGGAPHEVLEDAK
                     EGYLEIDDPKAPIPARSAWLLLRRFIAGAEEKYNTRTK"
     gene            2041..2700
                     /locus_tag="VGE59_03975"
     CDS             2041..2700
                     /locus_tag="VGE59_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY1074823.1"
                     /translation="MTRELRLGTAAVIITAALIAIFAFSAKSKPNVARIAQSIDTTGA
                     IARGPENAPITIIEYTDFQCPSCKQLYPTLETLLKEYDGKIRFIHRHFPLMDIHYNAY
                     DAAMASEAAKDQGRFWDMHNLLFERQGEWANAKNPNIVFERYASELGLSLPEYRTFVE
                     EQKGKDAIDRDVAEGETLGVTSTPTLFLNGKGMSGVPEYADLKLQILEILATSSPTPA
                     P"
     gene            2882..3253
                     /locus_tag="VGE59_03980"
     CDS             2882..3253
                     /locus_tag="VGE59_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074824.1"
                     /translation="MNQNKVPVFPWELNVMIAAILVAIVYGVATNDAVGMAAILAATY
                     VGLSVTTKQGKTALAGMAVICCGTLAFAIVNKAGIHWSGVACLTYFVVTFAAAGALAV
                     QINNREIAAAFGNRNRQIQTF"
     gene            3479..3832
                     /locus_tag="VGE59_03985"
     CDS             3479..3832
                     /locus_tag="VGE59_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074825.1"
                     /translation="MNEKEKELRVRRLKTMLLFEQAKFSLLAGLVLTGLLYGLGGQTI
                     PEIPALVGKFCYCSILAFVLMNVRMPLRRRPSHQESHQPKDRFGTDDLDRDPGGLLGH
                     WGSESDLDTDQDDDL"
     gene            3948..5465
                     /locus_tag="VGE59_03990"
     CDS             3948..5465
                     /locus_tag="VGE59_03990"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY1074826.1"
                     /translation="MQELTTLKQRLSEITHLRSALAILQWDQEVYMPEEGSRFRGETL
                     AYLAGLNHTKFTALDSDAILTKLHQAVQVGTLQGDDAVIVEEVWRDFEREQKLPTDFV
                     EALTKLTSEANSVWVKARKANDFSIFAPYLEKIVAMSREQAKLLGYKDSPYDALLDMY
                     EPGATTKEVATKLEELKTFLVPFIQKLTQSPNQPDPSVLLGNYPVEKQIDFNKQVAEA
                     LGFQFSCGRVDVSAHPFCTNFNPQDVRFTTRYNTEDIWGSVGSTIHEVGHGLYEQGLN
                     PEHFGTPLAEYISLGIHESQSRMWENQVGKSKPFWQYFYPKLQAEFPEPFGALSFEDF
                     YRAINTVTPSLIRTESDEVTYNLHIIIRFEIEKELIEGTLEVQDLPAVWNKKYSEYLG
                     IDVPDDAHGVLQDVHWSHGSFGYFPTYTLGNLYAAQIFAQAKKEIQQLDEKLARGEFH
                     YLLDWLRTKIHIHGKRFTAGALIQEITGTALSSDHFTRYLQDKYTEIYPAEGDTL"
     gene            5462..5926
                     /locus_tag="VGE59_03995"
     CDS             5462..5926
                     /locus_tag="VGE59_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY1074827.1"
                     /translation="MNRPLIIGIVAVLAVVAVAFAQRNRTSTISPTNTPTAQVTVTTT
                     LETTATPSATTVTTVSPTTTTTHDVTFNGQAFSPRSLTIKVGDTVRFLNNGSQSIWVA
                     SDPHPVHTKYSAFDAERGYAPGETYSFTFTKAGTWTYHNHLNSSQTGTIVVE"
     gene            6004..6924
                     /locus_tag="VGE59_04000"
     CDS             6004..6924
                     /locus_tag="VGE59_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY1074828.1"
                     /translation="MVTIFHKTIKDTEPKVLKESKTGVWVCLEEPTESEVTAVAKAHA
                     LDASLIKDALDLYEVPRIEREDGVLYVYTRVPFQEGTSVMTVPLLIALTGSAVITVSM
                     RQLPVFAKFKEGKLPFTTTQRIKLFLQFFTEINASYSRFLTNMSRRVRAASVQLESIT
                     NRHVVQLVSFENILNDFLAALVPTSALLNTLLSGKTLKLYATDEDLVEDIFLTNGQLI
                     ELAKSNLKTIVNVREAYSTIMGNNLNQVLKLLTSLTILMTVPMIISSFFGMNVALPFA
                     QSPYAFAGIVSITLLITLIIFLIFNRNRWL"
     gene            complement(6928..>7415)
                     /locus_tag="VGE59_04005"
     CDS             complement(6928..>7415)
                     /locus_tag="VGE59_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY1074829.1"
                     /translation="PWLIVGLFGAASVSFLMSRFERALSEDLSLAFFLPAIVYMSDAV
                     GTQTETMIVRNMATKRIRFAVYLVKEFFLGLALGVIFGILLGGFAFVWLHDSKVALSV
                     GLAMFANILVAPLIALAIPTFIRHAHKDPALGAGPFTTIIQDAISVVIYFMIATAIIF
                     H"
BASE COUNT         2029 a         1945 c         1773 g         1668 t
ORIGIN      
        1 cctcgatcta cgcgccggtg cgcttcgcct gcccgcccga ggcgatcgtc atgacgctca
       61 ccccggtatc gctgaacgga cagaccaccg gaatcgggta tccttgacag gttgccgcgc
      121 agcagcaacg gggtatggcg cagcttggta gcgcgcgtcg ttcgggacga cgaggtcgtg
      181 ggttcgaatc ccgccagccc gaccagacaa aaaacatctg caatgaacag atgttttttg
      241 tctggtgaat ctagtctaag tgaagcaaat ttcttctctt acctccacaa tacactttat
      301 gaaagtgaac tctggggtag ggagtaggca cacaagcgcc ttaacgaatc tccccatccg
      361 gacagtatga tgtcagcagc gagtcaccgc ataaccattc tccgcaccgc ctgaggccac
      421 acgatgaacg cgccgggtag ataccattgg caccagctga tgcgggtgag gcttagcacc
      481 agcagaagta ccagcggtac ggccacaaac cacgacgaag tggcgagaga cagcccgaac
      541 gtccacagcg caaagccgcc atggaatggg aaccttgcca gccccggccc gactagcgcc
      601 acactgagta tctggaagag gccgtagaga cagagaccaa ccccgaccca gcggtagtag
      661 tccggcaaag ccggcgatgc ggtcagcgcg gacgtatccg cgctatacca cagcagggcc
      721 accacgacag ccatcttcag cacgctccgc aggagggcga acttctgctg ccggacttcc
      781 ggacagacgg tgcgccactt cggatcgaag tagcaaatcc gacacctttc cgactgacgg
      841 taccatccgt agtagcctgc gaagcaggcg aaggcgagcg cgcacagcat tcgaaccgca
      901 agctcaatgg gcacgtgaat cctccggatt cagacagtaa agagcacgag cgctcccagt
      961 tctgcgaaca caagccaagg cttatgtgca tagaccgaat gcaacgtttc gtgagtcata
     1021 caatactaaa aatgtatact tttgtcaatg ctgttatttt aaactactgt ctacccaaaa
     1081 agatacataa aactttgtga cttgatatac cgataaatac tatatatact aaacaacaaa
     1141 ccacttgagt gtgatttaag ggaggaaata agtggaagca atttccgagg gagtcgctac
     1201 tgaaggcaga ctcctcgcaa caaagagtcg aggcaaagcc ctcgaacgcg tctgcggcat
     1261 ctgtgagata cgaaacgtcg tccgggaggc atactgtcct atctgtaccg ccaagatccg
     1321 agaggtaaag actcatctgg cgcagatgaa acgaccctat accgaaggga atattgcgca
     1381 atcaattcgc cgacactggc gagtagccga cgcaccccag atggtatcaa tccggatcta
     1441 tgcccgggtt tggaataccg ctatcgaaac gatcatccag ctctgtgccc aagaggggtt
     1501 ggccacaaga gggacccggg agaagaaaaa ggaaccacac tatctgcctg tggcaacggt
     1561 gatttttctc gacatgctgc tcgaagctgg catcgccgta cagacgaagg ttcgtcctcg
     1621 ctgcacgttg tgccaccatc cggcagcggg caaccttccg ctctgcattc ggcacctggc
     1681 cgtctgggag tggatgtgcg agaagatcgg aaaaccgacg tacatgcttc cgctcattaa
     1741 gctgggacaa ggagaagcga tcagtcttgg gagcttggcg cttctcaccg gaggagcgcc
     1801 ccacgaggtc ctcgaggacg ccaaagaggg ctaccttgag atcgacgatc cgaaagcccc
     1861 gattcccgca aggagtgctt ggttgctctt gcgacggttt attgccggag cagaagagaa
     1921 gtacaacacg cgaacaaagt aacctgccca gcggggcatg gccgaaagcc tggcgcaagc
     1981 caggcttttt cttgcacccc aaagccccaa agagtatgct gcggcgagaa gggaagaacc
     2041 atgacacgag aactacggct cggcacggcg gcagtaatta ttaccgcagc attaattgca
     2101 atttttgcgt ttagcgcaaa atctaagcca aatgtggcac gtattgccca gtctattgat
     2161 accaccggag caattgcgcg cggacccgaa aatgcaccga tcacgattat tgaatatacc
     2221 gactttcaat gtccttcctg taaacagctc tatcccaccc ttgaaacact tcttaaagaa
     2281 tacgatggta agattcggtt tatccaccgt cactttcctt taatggatat tcactataac
     2341 gcctacgacg cagccatggc gagcgaagca gccaaagacc aaggccgttt ttgggatatg
     2401 cacaaccttc tctttgaacg gcaaggagaa tgggccaacg caaaaaatcc gaatattgta
     2461 tttgaacgct acgcgtctga gctaggactt tcactcccgg aataccgtac gtttgtagaa
     2521 gaacaaaaag gcaaagatgc catcgatcga gacgtcgcgg agggagagac actgggagtc
     2581 acctcgaccc ccacactctt tttgaacgga aaaggaatgt ctggtgtacc cgaatatgcg
     2641 gatttaaaac tgcagatttt agaaatcttg gctacttcta gcccgacgcc cgcgccgtag
     2701 taagccttga cagaatagac tttttgtggt atatttcttg cgtcaatgag agaagcttcg
     2761 accgctcttc tcacgcacct cacaaccacc tggaagaggc atcacttttc gatgcccttc
     2821 ccccaggact ccttaagtcg gcagttgagc cgtcaggaag gagccgtgga gggaagaacc
     2881 cgtgaatcag aataaagtcc ccgtgttccc ctgggagcta aacgtgatga tcgccgcgat
     2941 cctcgtggct atcgtgtacg gcgtggctac caacgacgcc gtcggcatgg ccgcgatcct
     3001 tgcggcgacc tacgtcggcc tgtccgttac gaccaagcaa ggcaagacgg ccctggccgg
     3061 gatggcggtt atctgctgcg gcacgctcgc gtttgcgatc gtgaacaagg cgggtatcca
     3121 ctggagtgga gtggcgtgcc tcacgtactt cgtggtcacg ttcgcggccg ccggagctct
     3181 agcggtgcag atcaacaacc gtgaaatcgc cgccgcgttc ggcaaccgga accggcagat
     3241 ccagactttc tgatctgcct ttggcggaag gccagggtac ccgacaacag gatcgctgtc
     3301 cggattcgtc cggacagcac cacctcagag tttacttttc taagccgact ctgaagatgg
     3361 tattctcgct accgtcctgc tggctatcag ccacgtactg gtaatgttgc tttaatttat
     3421 tacagcaacg ccggcaggac cgtggcggga atcttcaccc cctgcccgag acaagcacat
     3481 gaacgaaaaa gaaaaagagc tacgggtaag acgactcaaa acgatgctct tgttcgagca
     3541 ggccaagttc tcacttttgg cagggctcgt tttgacagga cttctctacg gcctgggtgg
     3601 acagacgatt cctgagatac cagcattagt cggaaagttc tgctactgct ctatcctggc
     3661 ctttgtgctc atgaacgtga gaatgccgct caggcgaagg ccgagtcacc aggagtcgca
     3721 ccaacctaaa gaccgattcg gtacggacga cctcgatcga gatccaggag ggctactcgg
     3781 ccactgggga agtgagtccg acctcgacac agatcaagac gatgaccttt aggtctgccg
     3841 cccctcaaca ctcaaccacc caaaccagct tcggctgggg cgggtggttt tgcattttat
     3901 agaaagtctc taatgacatg ttattaaatt aggcgtatcc tcaagccatg caagaactta
     3961 ctactctcaa acaacgactg agcgaaatta cgcatctgcg atctgcttta gctatcctcc
     4021 agtgggacca agaggtgtac atgccagaag agggaagtcg ctttcgtgga gaaacgttgg
     4081 cctacctcgc ggggcttaac cacacaaaat ttaccgcttt agatagcgat gcgattctta
     4141 ccaagctcca tcaagccgtc caggtgggaa cactacaagg tgatgacgcc gtaatcgtag
     4201 aagaagtgtg gcgtgacttt gaacgggagc aaaaactacc taccgatttt gtagaagcac
     4261 ttacgaaact caccagtgaa gccaacagtg tgtgggtaaa agcaagaaaa gccaatgact
     4321 tttcgatttt tgcgccgtat ttagaaaaaa tagtggcaat gagccgagag caagcgaagc
     4381 tccttggata taaagattcg ccttatgatg cgttgttaga tatgtatgaa cccggcgcaa
     4441 ccacaaaaga ggtggcgact aagcttgaag aacttaaaac atttctggtg ccgtttatcc
     4501 aaaaacttac acagagtccg aatcaacccg acccttcagt gctgttaggg aactatcccg
     4561 tagaaaagca aattgacttt aataagcagg tagccgaagc gttaggcttt cagttttcct
     4621 gcggccgggt cgatgtaagc gcccacccgt tctgcacaaa ctttaatccc caagatgtgc
     4681 gctttaccac ccgctataat actgaagata tttggggatc ggtcgggtca actatccatg
     4741 aagttgggca cggtctctat gaacaaggtt tgaatcccga gcactttggg actccgttag
     4801 cggaatacat ctcactcggt attcacgaat cacaatcacg tatgtgggag aatcaggtag
     4861 ggaagagtaa gccgttctgg cagtattttt atcctaaact ccaagcggag tttcctgagc
     4921 catttggagc actttcgttt gaagactttt accgtgccat taacactgtg acaccttcgc
     4981 tcattcggac agaatcagac gaagtcacct acaacttgca tattattatc cgctttgaaa
     5041 ttgaaaaaga gttgattgag gggactttgg aagttcaaga cttgccagcg gtctggaata
     5101 aaaagtatag tgaatactta ggaattgatg tgcccgatga tgctcacggc gtactccagg
     5161 atgtccactg gtcacacggg tcattcggtt actttcctac ctatacactc ggaaatctct
     5221 atgctgcaca aatctttgct caagcgaaaa aagaaattca gcagctagat gagaagttag
     5281 cccgaggaga attccactat cttttggact ggctccgcac aaaaatccat atccatggca
     5341 aacgattcac agcaggtgca ttgattcaag aaatcaccgg gacagcactc tcaagcgacc
     5401 actttactcg ttatttacaa gacaagtaca ccgaaattta cccagcagaa ggagacacct
     5461 tatgaatcga ccactcatta ttggcatcgt tgcagttctt gcggttgtcg ccgtagcctt
     5521 cgcacaacga aaccgcacct caaccatctc cccaacaaac acaccaaccg cgcaggtaac
     5581 cgtaaccacc acgctcgaaa cgactgcaac acccagcgcg accaccgtaa ctacggtaag
     5641 tccaaccacc acaaccacac acgacgttac gtttaatggc caggcattta gtcctcgtag
     5701 tcttacgatt aaagtagggg atacggttcg attcctcaat aacggatccc agtcaatttg
     5761 ggttgcctca gacccacacc ctgtacacac taagtatagt gcttttgatg ctgagcgcgg
     5821 atatgctccg ggagagactt actcctttac gttcaccaaa gcaggaactt ggacatatca
     5881 taaccatctg aattcaagcc aaacaggtac tattgtcgta gaatagctgg ttgaccacta
     5941 gttagatata tactaaaata ggggggtata ttttcgcgtt tttacatagg gtaaaggggt
     6001 gctatggtca ctatttttca caagacaatt aaagatacgg aacccaaggt cttgaaagaa
     6061 tccaagacgg gcgtttgggt ctgtctcgaa gaaccaactg aatctgaagt caccgccgta
     6121 gccaaagctc atgcccttga tgcgagctta attaaagatg cgctcgactt gtatgaagta
     6181 cctcgtatag aacgcgaaga tggcgtactc tatgtctata cacgtgttcc attccaggaa
     6241 ggtacctctg ttatgactgt acctcttctt atagcgctca ctggttccgc cgttatcact
     6301 gtctccatgc gacaacttcc cgtctttgcc aaatttaaag aaggaaagtt accctttacg
     6361 acgacacagc ggatcaaact cttccttcag ttctttaccg aaatcaatgc cagttacagc
     6421 cgatttttaa ccaatatgag ccgacgagtt cgcgccgctt ccgtacaatt agaatccatt
     6481 accaaccgcc acgttgtaca gctcgtttcg ttcgagaata ttctcaacga ctttctggca
     6541 gcattagttc ctacaagcgc tcttttgaat accttactct caggcaaaac cctcaagctc
     6601 tatgcgacag acgaagattt agtcgaagat attttcctga cgaatggtca gttaattgag
     6661 cttgcaaaat caaaccttaa gaccattgtg aatgtccgtg aggcctactc aacgattatg
     6721 gggaataatc tcaaccaggt gttaaaactc ctcacctcac tcactatctt aatgaccgtg
     6781 ccaatgatca tctcgagctt ctttggcatg aacgttgcgc ttccctttgc ccaatcaccc
     6841 tatgcttttg ccgggatcgt gtcgatcacg ctcctgatta cgctcattat ttttcttatc
     6901 tttaaccgta accgctggct atagtcttta gtgaaaaata attgccgttg cgatcataaa
     6961 gtagattaca acgctgatag catcctgaat aatagtcgtg aatggtcccg ctcctaatgc
     7021 gggatcttta tgagcgtggc gaataaaagt aggaatagct aatgcaataa ggggagcaac
     7081 taaaatattc gcaaacatgg ctaacccgac tgatagagca actttcgagt catggagcca
     7141 cacgaaagca aaccctccca ataaaatacc aaaaatgaca ccaagcgcga gtcccaaaaa
     7201 gaattctttc acaagataca cggcgaaacg gattcgcttc gtagccatat tccgcacaat
     7261 cattgtttcg gtctgagtcc ccaccgcatc gctcatatag acaattgccg gtaagaaaaa
     7321 ggcgagcgag agatcttcgc ttaaagcccg ttcaaaacga gacattaaaa acgaaaccga
     7381 cgcagcacca aataagccaa cgattaacca gggta
//
LOCUS       DASWYB010000069         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_715244, whole genome shotgun sequence.
ACCESSION   DASWYB010000069 DASWYB010000000
VERSION     DASWYB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715244"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VGE59_04010"
     CDS             complement(<1..864)
                     /locus_tag="VGE59_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1074830.1"
                     /translation="MLKTLIKDKIELSHKSIINELRGYRNRPEDAICEYIWNSFDAQS
                     TTVNINYDFINGTESAVAFGYPSLSVSDNGTGWDLSNTQTAKLFLDSQKSLHKNRFKS
                     IPHGSKGVGRFTFYCFASKAIWESHFNGQSYQLTLERESLASYEIDSSKLGPTDQNGT
                     TVRFEVDNTLLNESFFEDVLPARLLEKFAWFLIEHPEKQININGNPLDLNSIIKNRKE
                     ESLIIDGKTVLLDLVQWNRSLADQENSRAYYIGNDSEEICKYPTGLNNKADDFYHSAF
                     VRSASFDTVLFD"
     gene            complement(1139..1214)
                     /locus_tag="VGE59_04015"
     tRNA            complement(1139..1214)
                     /locus_tag="VGE59_04015"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1179..1181),aa:Ala,seq:ggc)
     gene            complement(1280..2326)
                     /locus_tag="VGE59_04020"
     CDS             complement(1280..2326)
                     /locus_tag="VGE59_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074831.1"
                     /translation="MLLVFQPVRHGIAYAAFTPTSELVYDGFLTIDEWSSRHLKQRFD
                     AELLTKVRGHGRVFTKLGIILPFADINFRGAKPATSVSLKQLATTSLRQTLLDPARLT
                     YEAAKSAWPHLPQFFLSDTYLSDQVDRTFGLPPFSYEMNKTLNSVPYLVHSYGHRANL
                     ASVKDAKVVLSLYVGEQTSIALFEDEKLKDATISHAPFSSVMGLHSPGSLDVGFVTEL
                     VQKKRPAEVTNLFISQSGLQPMTETKLSIDDLIHIAGLVPRKDMEHLDKLSIEAIEWI
                     ELSVRNFVRSLRHSLGGFLTYSGKIDSLVINTSVVGATSPLWKLLLSGSLSKLSVHYC
                     DISLLQAACRDLEA"
     gene            complement(2339..>3016)
                     /locus_tag="VGE59_04025"
     CDS             complement(2339..>3016)
                     /locus_tag="VGE59_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074832.1"
                     /translation="CNESITLGSLFPLDPTWRDVSTESFLRTVLHESTSDDIHDIVTP
                     LKRGHVFHGPAVGSEDLFLIANHDLHGLDTVGDTLQAWGEHPLMTMFCDHNPDAAICQ
                     SLDKYRHIVSVTSRQSYHDAYFGSSLRHPYLEQIKHSSPTTLLEMLNSPHYGYPSKPY
                     RTVALLDEELNADFDWLLSSYLKQQRQDAYSCLFIDPRAVEGSVVISNMKFDPNTPLA
                     SQLHVYA"
BASE COUNT          883 a          633 c          669 g          831 t
ORIGIN      
        1 gtcaaataag accgtgtcaa aagatgcgct cctaacaaac gctgaatggt aaaagtcatc
       61 cgccttattg tttaaacccg tagggtattt acaaatttct tcactatcat ttccaatata
      121 gtaagctcta gagttttcct gatccgctaa cgatctattc cactgaacta aatctaataa
      181 aaccgtttta ccgtcaataa taaggctctc ttcttttcga ttctttatta ttgagttaag
      241 atccaatgga tttccattta tattgatttg tttttcggga tgctctatta aaaaccaagc
      301 aaatttttcc aataatcttg cgggaagcac atcttcaaaa aatgactcat ttagtagggt
      361 gttatcaact tcaaatctga ctgtagttcc attttgatct gttggaccta atttactaga
      421 atctatttcg tagctagcaa ggctttctct ttcgagtgtc aattggtagc tttgtccatt
      481 aaaatgactt tcccatatgg ccttacttgc aaaacaatag aatgtaaatc ggcctacgcc
      541 cttacttccg tgaggtatgc ttttaaaacg gtttttatgt aaactttttt gggagtctag
      601 aaaaagtttg gcggtttgcg tattgcttaa atcccagcca gttccattgt cagatactga
      661 aagactagga tacccaaaag ctaccgcaga ctctgtacca tttataaaat catagttaat
      721 atttactgtt gtagactgtg catcaaaaga attccaaata tactcacaaa ttgcatcctc
      781 aggacgattt ctgtaacctc taagttcatt aataattgac ttgtggctaa gttcaatttt
      841 gtcttttatc aacgttttta gcatcgttcg tttataatcg cacttttctt ggttattctc
      901 aagaacacaa aaatcaccac ctatctacca aacgcaaaaa gcaggcgttt ctcaacctgc
      961 tttctaccat ggtgtaccgt accggcgtac atcggccaca gtgtggcctg gtataattcc
     1021 cgacgcttcg ctctgggctt acgggcagag cccttcagga attatctgaa ccgcgcggtt
     1081 ttgaaaccgc atataatgct accagacgaa aaaagccctt ttcgaagcta ctttcgtctg
     1141 gtggacctta ggagactcga actcctgacc ccttccatgc catggaagtg ctctaccaac
     1201 tgagctaaag gccctaaaaa tgtaaggtgc aagatcactc tgtattatac ggtattgccc
     1261 taaaaacgac aagatcgcgc taggcttcta agtcgcggca ggctgcttga agaagtgaga
     1321 tatcgcagta atgtacagag agcttgctta aacttcccga caacaacaat ttccagaggg
     1381 ggcttgttgc acccacgacg gaggtattaa taaccaatga atcaatcttg ccggaatagg
     1441 tgagaaaccc accaagcgag tgacgcaacg atcgaacaaa gttacgtacc gagagttcga
     1501 tccattcaat tgcttcgatt gagagcttgt ctaaatgttc catatctttc cgtggcacta
     1561 agccagcaat gtggattaag tcatcgattg agagtttggt ttctgtcatg ggctggagac
     1621 cactttggga gataaataag ttcgtcacct ctgctggacg tttcttttgc acaagctcgg
     1681 ttacaaaccc aacatcgaga ctacctggac tatgtaaccc cataacagaa gaaaagggag
     1741 cgtgagagat tgtggcatct ttaagctttt catcttcaaa aagagcaata cttgtctgct
     1801 caccaacata taagctaagt actactttgg cgtctttaac cgatgcaagg ttggcacggt
     1861 gaccataact atgcacaagg tagggaacac tattgagagt cttattcatt tcgtaagaaa
     1921 aaggcgggag gccgaacgtg cggtcgactt gatctgaaag atatgtgtcg gaaagaaaaa
     1981 attgtggtaa gtgaggccaa gctgattttg ccgcttcgta ggttaaacga gcgggatcta
     2041 gcaatgtttg gcgcagagaa gtagttgcta attgtttaag acttacgctg gtagctggct
     2101 tggcaccacg aaaattgatg tcggcaaatg gcaaaataat gccgagtttg gtaaatacac
     2161 ggccatgacc gcgcactttg gtaaggagct ccgcatcaaa ccgctgctta aggtggcgag
     2221 atgaccattc atcaatagtg agaaatccgt cgtacactaa ttccgaagtg ggagtaaatg
     2281 cagcgtaggc aataccgtga cggacaggtt ggaatactaa taacatggaa cctccgggtt
     2341 aggcatatac atgaagctga gaagccaggg gagtattggg atcaaatttc atattactga
     2401 taacaacact gccttctaca gcacgcggat cgatgaatag gcatgagtag gcatcttggc
     2461 gttgctgctt gagataggag ctcaaaagcc aatcaaaatc tgcgtttaat tcttcgtcaa
     2521 gcaaagcaac ggtgcgatac ggttttgatg gatacccata atgaggggaa ttaagcatct
     2581 ctaagagcgt agtaggggaa gagtgtttaa tttgctccag ataggggtgg cgtaaagacg
     2641 agccaaagta ggcatcatga taactttgtc gtgaggtcac ggatacaata tgacgatact
     2701 tatcgagaga ttggcagatg gcggcatcgg gattatgatc acaaaacatt gtcatgagcg
     2761 gatgttcgcc ccatgcctgt agcgtatctc ccaccgtatc aagaccatga agatcgtggt
     2821 tggcaataag gaacaaatct tcactcccaa ccgctggccc atgaaacaca tggccacgct
     2881 tgagaggagt tacaatgtcg tggatgtcgt cagaggtcga ttcatgtaac accgttcgaa
     2941 ggaacgattc tgttgatacg tcacgccagg tagggtcgag cggaaagaga gaaccaagcg
     3001 taatagattc attaca
//
LOCUS       DASWYB010000070         8767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_1004345, whole genome shotgun sequence.
ACCESSION   DASWYB010000070 DASWYB010000000
VERSION     DASWYB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004345"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..352)
                     /locus_tag="VGE59_04030"
     CDS             complement(86..352)
                     /locus_tag="VGE59_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074833.1"
                     /translation="MIQTLKLYSQLLLQQLVDRIARKQVFTLRAAYSKPSHAPYQELD
                     YTQSNRAVLITELDEYGDETPVAVAFLKDSEVVVRKLAELCEAE"
     gene            complement(405..764)
                     /locus_tag="VGE59_04035"
     CDS             complement(405..764)
                     /locus_tag="VGE59_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074834.1"
                     /translation="MPTIRSVGDYHDFYTRREAESSVRSLGSTLGQLVWFLVGFLESL
                     LVLRFVFRLFGAADTSLTTWIYRLTEWLVTPFLRIVSTVQSGGLVLEIATLLTMFSVA
                     LIGILINALIESLRPTI"
     gene            complement(1694..2076)
                     /gene="rnpB"
                     /locus_tag="VGE59_04040"
     ncRNA           complement(1694..2076)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGE59_04040"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2166..2783)
                     /locus_tag="VGE59_04045"
     CDS             complement(2166..2783)
                     /locus_tag="VGE59_04045"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY1074835.1"
                     /translation="MKTTAGWEYELKHQQAGKRVIAGLDEVGRGCWAGPLVACAYVFH
                     NVPCDIVVGDSKTFSEKQRKELAFKLVELGVSSLGEVSAEEIDALGLQQAQYLAYERA
                     LQGLPIAPEVVLLDGRPWKTELFFCDAVVDGDAKIASIAAASIVAKVYRDTYMREVAH
                     SEYPYYGFNQHVGYGTKVHQSALQDRGTCILHRQSFKPIKSIVSR"
     gene            2860..3258
                     /locus_tag="VGE59_04050"
     CDS             2860..3258
                     /locus_tag="VGE59_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074836.1"
                     /translation="MERKCWIVKYQPDMRAFIARVVESHTGLTPVTVDDSWELTHQDP
                     QECQGVILDSYQQNRYVADLILETLRILLRDRFLEIPIILTSTVDTDRQINETIMLQS
                     ERKVQIHFIKLNSPSWREGLQRILEPPTGE"
     gene            complement(3316..6207)
                     /gene="uvrA"
                     /locus_tag="VGE59_04055"
     CDS             complement(3316..6207)
                     /gene="uvrA"
                     /locus_tag="VGE59_04055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY1074837.1"
                     /translation="MHDNRPIKITGARQHNLKNVTISIPRNSLTVITGLSGSGKSSLA
                     FDTLFAEGQRRYVESLSSYARQFLDTLEKPEFDTIEGLSPTIAIDQKTASHNPRSTVG
                     TITEVYDFLRLLFSRCGTPHCPHHPKEELVAQTSEQILQRMLEDVKALGLPQKVVVYA
                     PLISDKRGEHKYALKTAQKAKIDRVRIDGTSMTIEEALTLDIDRNKRHSIEAEILTLG
                     IYSEDRETVKELEQGVLRALQLGNGLTILFNQNTGEEKFYSEHYTCPECGFSLPTIEP
                     RLFSFNSPHGACPVCQGLGTRLEFDQGLILPNPRLTLGEGAIRPWSRITSHSNWYQKS
                     LSTFAIRYKVSLTDTPVGELTEKQLHVLLYGDMDNASIPEEEQFEGIIPNLRRRYDET
                     DSDYLRQEIEKYMVVRTCPSCHGQRLRAEILGITVQGKNIVDINGMTIEELSAFLTGH
                     TQNDRNQMVYEQLAREITQRLGFLLDVGLGYLTLDRSASTLAGGEAQRIRLATQLGSQ
                     LMGVIYVLDEPSIGLHVRDHERLLKTLFMLRDIGNTVVVVEHDEQTMKAADYIIDIGP
                     GAGEKGGQLIAAGTPDEVMSDTNSLTGAYLSGRNYIEVPPKRRKAADKWLTVHGAEEF
                     NLKNVTVKFPLERFVVVAGVSGSGKSTLVADILARALSVKFHRAKVVPGKHQKITGME
                     HIDKVIDIDQSPIGRTPRSNPVTYTGIFGPIRELFAETPAAIERNFDAGHFSFNLRGG
                     RCETCKGDGVLKIEMNFLPDVYVTCSECKGRRYNQEAMEVLYNGKSIADVLDMTIDEA
                     VEFFKQDELIHHKLSVLQKVGLGYMRLGQPATTLSGGEAQRIKLATELSRQSTGHTLY
                     ILDEPTTGLHFEDNKRLLAVLQALVDKGNSVIVVEHDMDVIKSADWIIDIGPDGGDKG
                     GSVVAEGTPEQVAKVTASHTGRALAEVLAASKQPATSEKVPLAEPLSIA"
     gene            6448..6882
                     /locus_tag="VGE59_04060"
     CDS             6448..6882
                     /locus_tag="VGE59_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY1074838.1"
                     /translation="MNRERPIAGPGTIIGGNVVLTGILKDAGDIAIHGKIEGEVTSER
                     SIVIGETADVKGPVSGQIVTVAGTIRGSVDAGQKLEILPTGKIFGSISTRELIVRSGA
                     VFIGKSTMPLEENVEEAHDESMEHSARMNEYEHAGHAVVEED"
     gene            6910..7725
                     /locus_tag="VGE59_04065"
     CDS             6910..7725
                     /locus_tag="VGE59_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY1074839.1"
                     /translation="MYYYIIDPPKGSPTAKIAQKLQELITPMGISGEIAIANPARSAE
                     ELTYMGIDKGYTTIVAVGGEELANTVATILLNESREKIAMGIIPINAGPLISQMVGVA
                     NNDLRAAAEIIKQRHLDLVDMVQISQKRYMLTEAYIVAPRTVKVTLDVDGRVKAELEA
                     DYAHLSHDLVLTLQVRQPQGFLKRMMGGAPKPEQYASQFHGKQIRILAHEPLPIMIAD
                     QVVAKTPTTFTKIPGALKLITLRAILPQKGMSEIVAKNTHHHTEDPVPFASKK"
     gene            7914..8396
                     /gene="infC"
                     /locus_tag="VGE59_04070"
     CDS             7914..8396
                     /gene="infC"
                     /locus_tag="VGE59_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY1074840.1"
                     /translation="MNERINSPEVFLIGPKGEPFGVVSNDQAQFLAFDANIDLVEINP
                     NRVPPIVKMMDYGKYRYEIEKKQRESKVKAKASELKEVRLTRKIDKHDLETKAKRAKD
                     WLEHGDKVRVYLQLMGREYMFADAARETINHFRELIDGVFEQAPNQMGNRIIAIIRKK
                     "
     gene            8454..8660
                     /locus_tag="VGE59_04075"
     CDS             8454..8660
                     /locus_tag="VGE59_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY1074841.1"
                     /translation="MKLKTNKTASKRIANITGTGKAMRLKMSAQHLAPGKSRRTLKNS
                     KFLLPISGADIKRMKRLLPYAKIK"
     gene            8670..>8767
                     /locus_tag="VGE59_04080"
     CDS             8670..>8767
                     /locus_tag="VGE59_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY1074842.1"
                     /translation="MARVKTGSTTHRRHKKILERTKGFKLGRNSLFR"
BASE COUNT         2440 a         2140 c         1991 g         2196 t
ORIGIN      
        1 ttaattttgt aaattataac taacacagaa tacaaaaaag gagctagcca catgactaac
       61 tcctttttaa gagaggacaa taaccttatt ccgcttcaca caactcagct aactttcgca
      121 ctaccacttc tgaatctttc agaaacgcta cggctacggg agtttcatct ccatattcgt
      181 cgagttccgt tattaagacc gctcgattgc tttgtgtata gtcaagctct tgatagggag
      241 cgtggctcgg ctttgaatat gcggcacgaa gggtaaacac ctgcttgcga gcaattcgat
      301 caacgagctg ttggagtaat aactgggagt agagttttaa tgtctgaatc atgggaactt
      361 cctttcactt tgtagttttt atacttgcgt tcaggaacgg tttttcatat cgttggcctt
      421 aaagattcaa tgagcgcatt gatgaggatt ccaatcaacg caactgagaa cattgtaagg
      481 agtgtggcaa tttcgagcac aaggccgccc gactgaaccg ttgaaacgat tctaagaaac
      541 ggagtaacga gccattcagt aagacgatat atccaagttg ttaatgaggt atccgctgcg
      601 ccaaaaaggc gaaaaacgaa tctgagtacg agaagagact caagaaatcc gacaagaaac
      661 caaaccaatt gtccaagcgt tgagcccagg ctacgtacgg aactctctgc ctcgcgacgg
      721 gtataaaagt cgtgataatc accgacggag cgaattgttg gcaccttacc tcctttcttg
      781 aagcaccctt gtactgacta actgtttcat cctcaagctt cctgcctatc ataaaagtat
      841 ccgggctctg cttcatcggg agatacgcca aaaaaccgaa accgcttatt tgccgaatta
      901 gagaaagcga caagttcaaa attacgaact caagataaat atatttacag gcataaagaa
      961 tatatgctta gaatcgctgt acaaaaaatt agttcgcgca gtgccattat ttagaactct
     1021 acgacaagac aatgcggggg aaatacacta ggattagaca tgtcatcccg caacgtatct
     1081 ggcattaacc aattcgatta attcttgtat cggggaacgt gttcatgaag ctgtggctct
     1141 atggagcacc ccacacctaa aaagaaaaag ggttagcatt gcacagactc atacgtagcg
     1201 gagtataccg aacgtcttcc tttgccacca tggtacggga ggacgttttt agataaggag
     1261 ttatttttca tagtgaaaca tttctttgag atatcattat aaaaagctat cgctttgttg
     1321 gaggacaggg gacttaattt ccggaagcag tcccctaccc tccaactcct cccacccccg
     1381 caccggacaa ggagaatctt ttgatatgaa aatgccactc tttaattaag tattctgtat
     1441 gagcgtgcag gggttgggag tggtgaggga tagggggccg cttcgcaagt aggccccctt
     1501 gtcccttacc cccggccgga ggccaaaacc acttaattca gattgccgcg atgcgctcgc
     1561 aatgacatgc aatccagatc ctgaaataag ttcaggagga tatacatttt agacctctcg
     1621 ctgattcgag atgccacaca aagcttttga gcatcaaaaa aaaactcctg ccatttggaa
     1681 ggagtttttt aatgcttcaa gatgttcgat aagccgagtt ttgtctgacc ataaggcgag
     1741 acggcaatct atctaagcgt tcgggcccaa aaggcgtctg gcgagtaacc attgacgata
     1801 aagggtccat cgaacttgca ctacgctggg ttttccaacg agctttgtca ccaaagccgc
     1861 cgtgggctct taccctcacc ttattacgag aataaggcta tcccttgcgg gacacgattt
     1921 caaccttacc tgtaccctta cgggccattg gcggtgtatt ttctgttgca ctttccctag
     1981 ggtctcccct ggtcgccgtt aacgacagcg ctactctgca gtgctcggac tttcctcaat
     2041 ccagtcaagc tggatcgtag ccgcctaaac atcttgaagc gcctcaataa tagcataaaa
     2101 tcattacttt gtcgagtcag aatactcatc tgctcgatac agattaagtt aaagttattt
     2161 cgcttttatc gggagacaat agatttaatc ggcttaaacg attgccgatg taaaatacag
     2221 gttccacgat cttgcagcgc cgattggtgg acttttgtac cataccccac gtgttgatta
     2281 aatccgtagt acgggtattc cgaatgcgcg acttctcgca tgtaggtgtc ccgataaacc
     2341 ttagcaacaa tcgaagctgc cgcgatggag gctatcttcg catcgccatc aaccactgcg
     2401 tcgcagaaaa ataattcagt tttccacgga cgaccatcca gaagtaccac ctcgggggca
     2461 ataggtagac cctgtaacgc ccgctcataa gccaaatact gcgcctgttg taagcctaag
     2521 gcgtcaatct cttcggctga tacttcacca agtgaagata cgcctaactc cacaagctta
     2581 aaggctaatt ctttacgttg cttctctgag aaggttttag agtcgcccac gacgatatcg
     2641 caggggacat tgtgaaatac ataagcgcac gccacaagtg gtccagccca gcagccacga
     2701 ccaacttcgt ccaggccagc aattacccgt ttaccagcct gttgatgctt taattcgtac
     2761 tcccacccag cggttgtttt catgctccta ccattgcaga aattaaagaa atttggtagt
     2821 atattcgtgc ttgaggctat gtgcacaagg agattgcaag tggaacggaa gtgctggatc
     2881 gtaaagtacc aacccgacat gcgagcattc atcgcaaggg tcgtcgaaag ccacaccggc
     2941 ctgacacccg ttacggtcga cgatagctgg gaactcaccc atcaagaccc gcaggaatgc
     3001 caaggagtaa tcctcgatag ctatcaacag aatcgctacg tcgcggatct tatcctggag
     3061 acgctccgca ttttgctgcg agatcggttt ctggagatac cgattatcct cacttcgacg
     3121 gtcgataccg accggcaaat caacgaaact ataatgctcc agagcgaacg gaaagttcag
     3181 atccacttca tcaaactgaa ctccccttcc tggcgagaag gtttgcagcg aattctcgag
     3241 ccacccaccg gcgaatagcc ggcacccatc gacagaatga aaagagcgcc tccgtggagg
     3301 cgctcaattc ttttattatg caatgcttaa aggctcggct agaggaactt tttcggaagt
     3361 tgctggctgc ttgctcgcgg caagcacttc agccaatgcc cgccccgtgt gagaagccgt
     3421 aacttttgcg acttgttcgg gagttccttc cgctactacc gaaccacctt tatctccccc
     3481 atcagggcca atatcaataa tccaatcagc tgatttaatg acgtccatgt catgctccac
     3541 cacaatcact gagttacctt tatcaacaag cgcctgtaac acagccaaga gacgtttgtt
     3601 atcttcaaag tgcagcccgg tcgtgggctc gtcgagaatg tagagcgtgt ggcccgtgga
     3661 ttgacgagaa agttcggttg ctaatttgat acgttgggct tccccacccg acagcgtagt
     3721 tgccggctga cctaatcgca tataacctaa tccgactttt tgcaacacag acagcttatg
     3781 gtgaatcagc tcgtcttgtt taaagaattc gacggcctcg tcaatcgtca tatctaagac
     3841 atcggcaatc gacttaccgt tatagagtac ttccatcgct tcttggttat aacggcgtcc
     3901 cttacattcc gaacaggtga cgtacacgtc aggcaagaag ttcatttcga tttttaaaac
     3961 gccatcaccc ttacaggttt cacagcgacc accacgcaag ttaaaactaa agtgtccagc
     4021 atcgaagttg cgttcaatcg cggcaggagt ctctgcaaag agttcgcgga ttggtccaaa
     4081 aatacccgta taggtaactg ggtttgatcg aggtgttcgg ccaatcggag attggtcaat
     4141 gtcgatcacc ttatcgatat gttccatacc ggtgattttt tgatgcttac ctggcactac
     4201 tttcgcccgg tggaatttaa cacttaaggc acgagcaaga atatcggcca caagggtcga
     4261 tttaccagat cccgaaacac ccgcaaccac aacaaaccgt tctaacggaa acttgaccgt
     4321 tacgttttta agattaaatt cctcggcccc atgcaccgtg agccacttat cagcagcctt
     4381 gcggcgtttt gggggaacct caatgtagtt acggccggat aaataagcgc ctgtgagaga
     4441 attcgtgtca ctcattactt cgtcgggtgt accggcagcg attaattggc cgcctttttc
     4501 gccagcgcca gggccgatat caataatgta gtcagccgct ttcatagttt gctcgtcgtg
     4561 ctccactacg acaacggtat taccaatatc gcgtaacata aaaagtgtct ttaagagacg
     4621 ttcatggtca cggacgtgca aaccaatact cggctcgtca agaacgtaaa tgacgcccat
     4681 aagctgcgat ccaagctggg ttgctaaacg aatacgctgc gcttctcccc ccgccaaagt
     4741 cgaagcacta cggtctagcg ttaagtaacc taagccaacg tcgagcaaaa agccgaggcg
     4801 ctgggtgatt tctcgagcga gttgctcgta gaccatttgg ttacgatcat tttgagtatg
     4861 acccgtcaga aaggcgctca attcctcgat tgtcatgcca ttaatatcaa caatattttt
     4921 accttggacg gtaataccta aaatttcggc acgcaaacgc tgaccatgac aacttggaca
     4981 ggttcgaaca accatgtatt tctcaatctc ttggcggaga tagtcagaat cagtttcgtc
     5041 gtatcgacgg cgcaagttgg gaataatacc ttcaaactgc tcttcttccg gaatcgaagc
     5101 attgtccata tccccataga gtaacacatg gagctgtttt tctgtcagct cgccaaccgg
     5161 agtatcggta agcgaaactt tataacggat ggcaaacgtt gagagcgact tctgatacca
     5221 gttactatgg ctcgtaatcc ggctccaggg gcggatagcg ccttcgccta gcgtaaggcg
     5281 tggattaggc aaaataagcc cttggtcgaa ctctaaacgg gtcccaagcc cctggcagac
     5341 cgggcaggcg ccgtgcgggc tattaaaaga gaatagccgt ggctcaattg tgggcagaga
     5401 aaagccacat tcggggcacg tataatgctc agaatagaat ttttcttcgc ccgtattctg
     5461 gttgaataaa atcgttaaac catttcccag ctggagagcg cgtaaaacac cttgctccag
     5521 ttctttcacg gtttcacggt cttcactgta aataccgagt gttaagatct cggcttcgat
     5581 tgagtgacgt ttattgcgat cgatgtcgag agttaaggct tcttcgatcg tcatactcgt
     5641 accgtcgata cggactcggt cgattttcgc tttttgggca gttttgagcg catatttgtg
     5701 ctcaccacgt ttatcagaaa tcagcggggc atatacaacc actttttgag gcaaacctaa
     5761 ggcttttaca tcttcaagca tgcgttgcaa aatttgctcg gatgtttgag caacgagctc
     5821 ttctttagga tgatgcgggc agtgaggcgt tccacatcga gaaaataaaa gacgcaagaa
     5881 gtcatacact tcggtaatcg ttcctaccgt tgaacgagga ttatgagaag cagtcttttg
     5941 atcgatcgct atggtcggcg aaaggccttc aatggtatca aactctggtt tttccaaagt
     6001 atctaaaaat tgacgcgcgt agctggagag actttcaacg tagcgtcgtt gtccttctgc
     6061 aaacagcgtg tcaaaggcga gtgaggattt tcctgaaccc gaaagtcccg tgataacagt
     6121 gagagaatta cggggaatac taatcgttac attcttgaga ttatgttggc gtgccccagt
     6181 aattttaatc ggacggttat cgtgcattcg tttcccttat tttagaacct attacaagtc
     6241 tcaatttagt atgttcgtcc cgagtaatat agcaaaaaaa tatagtttct gcaagtgtca
     6301 gagcccccct tttttataac ggagcatttt gctctcaagt gatttgattt ttccacttgg
     6361 gcatgatagg agtgataagt gtaacgactg cctcacacta ctcacgaggc gcctggcgtc
     6421 cccaggataa gaaaggagag gttttttatg aatcgcgaac gtcctattgc cggtcccggc
     6481 acaattattg gtggcaatgt tgtattaaca ggcatcctta aagatgccgg agatattgcc
     6541 atccacggta agattgaagg tgaagtaacg tcagaacgat caattgtgat tggtgaaaca
     6601 gccgatgtga aaggtcctgt atccggacaa atcgttactg ttgcgggcac tatccgtggc
     6661 tccgttgatg ctggccaaaa gctcgaaata ttacctacgg gaaagatatt cggcagcatt
     6721 tccacccgtg aacttatcgt ccgttcgggc gccgtgttta ttggaaagag caccatgccg
     6781 ctcgaagaaa atgtcgaaga agcccatgat gaatctatgg aacattctgc acgcatgaac
     6841 gaatacgaac acgccggcca tgccgtcgta gaagaagact aatttcccca ctcatttccc
     6901 gtgtgagcta tgtactatta tattattgat ccgccaaaag gatcgccaac agccaaaatc
     6961 gctcaaaaac tacaagagct tattaccccg atgggaattt cgggcgagat tgcgattgcc
     7021 aatccagctc gatcagccga agagctcacc tatatgggaa tagataaggg atacaccacg
     7081 atcgtagcgg taggtggcga agaattggcg aataccgtag ccactattct cctgaacgaa
     7141 tcccgggaaa aaattgccat gggaattatc cctataaacg ccgggccatt gatctctcaa
     7201 atggtcggtg tggccaataa tgacctacgc gccgcggcgg agattattaa gcaacgccat
     7261 cttgatctag tcgatatggt gcagatatcg caaaagcggt acatgctcac tgaggcgtat
     7321 atcgttgccc cacgaaccgt caaagtgaca ctcgacgttg atgggcgggt taaagcagaa
     7381 cttgaggccg attatgccca cctctcacat gatttggtcc tcacgcttca agtccgtcag
     7441 ccacaaggct ttctcaaacg catgatgggc ggagcgccaa agccggaaca atatgccagc
     7501 caattccacg gcaaacaaat tcgaattttg gcccacgaac cgctccccat tatgattgcc
     7561 gaccaagtcg tcgccaagac gccaacaacc tttactaaaa tccctggtgc gttgaaactg
     7621 ataacgctgc gtgctatact acctcaaaag ggtatgagcg aaattgtcgc aaaaaatacc
     7681 catcaccaca cggaagatcc cgtgccattt gcttcgaaga agtaggatgt gtcgtctctt
     7741 ataacttcta gtagcaacgg gaaggaaaat cttagcgaaa actgcgccgc aggaggaacc
     7801 atagccaaag ctatggggac gacgagaagc agtttgcagc cagattttcc ccggagctca
     7861 gaaattataa gagacgacac attcaagaag gagttcgcat aacagagcca cgcatcaacg
     7921 agcgcatcaa ttcgccagaa gtattcctta ttggaccaaa aggcgagcct tttggcgttg
     7981 tttccaacga ccaagctcaa tttcttgcct ttgacgccaa tattgatttg gtagaaatca
     8041 atcccaaccg tgttcctccc attgttaaaa tgatggacta cggcaagtat cgctacgaaa
     8101 tcgagaaaaa acagcgggaa tcgaaggtta aggcgaaagc ttccgaacta aaagaagttc
     8161 gcttaacccg taagatcgac aaacatgact tagaaaccaa ggcaaagcga gccaaagact
     8221 ggctcgagca cggtgataaa gtacgtgtct atttacagct tatgggccgt gaatacatgt
     8281 ttgctgatgc tgcccgtgaa accattaatc acttccgtga actgattgac ggcgtgttcg
     8341 aacaagcccc aaaccaaatg ggcaaccgga ttattgcaat tattcgcaaa aagtagtaga
     8401 attacagagt tatcgccgaa tatggcgaat attttgtgtc acaaaggacg cgtatgaaac
     8461 tcaagactaa taaaaccgca tccaaacgta tcgcgaacat caccggcacc ggcaaggcga
     8521 tgcgtttaaa aatgtctgcc caacacttgg cgcccggcaa gtcgcgtcgc accctcaaaa
     8581 atagcaagtt tttacttcct attagcggtg ccgatattaa acgtatgaag cggctcctgc
     8641 catacgctaa aattaaataa aggatcctca tggcacgcgt aaaaacaggt tcaactactc
     8701 atcgtcgcca caaaaagatt ttagagcgca ccaaaggctt caaacttggc cgcaatagtc
     8761 tttttcg
//
LOCUS       DASWYB010000071        29752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_758579, whole genome shotgun sequence.
ACCESSION   DASWYB010000071 DASWYB010000000
VERSION     DASWYB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29752
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758579"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VGE59_04085"
     CDS             <1..1585
                     /locus_tag="VGE59_04085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY1074843.1"
                     /translation="EDIVVVDCGIAFAGPEYPGIDALIPDVRYLEERKNNIRGMIFTH
                     GHEDHIGAVPYIWPKLGCPMYATPLAGGLIQHKLTEAGITNAPMTVVQAGDKLHFGKI
                     SVEMVRLTHSIPDVLGLAIRCPAGLFFYATDWRLEHTPALGKPADWTRIAQLAGEGIT
                     ALFSDSTNVDRPGYSPTERIIGEAFDRIFQDAKARIIIAQFASNINRVQQVFNSAKKF
                     GRKIALSGRSMETYVNIAMGLGYLKVPEGMLIDLRKVNTFDPHKVAILSTGSQGEEFS
                     SLVRMSEGAHRHVKLHPTDTVVVSASAIPGNEDQVERLLTNLYREGVNVINNKLVADV
                     HVSGHPSRDELKMMIALAQPQHFIPIHGSYPRMVDHGNIAVEMGVEPDKIIMMENGQI
                     VEFDEKGGRISAERIPMEDVMIDGKKIGDVGPIVIRDRKTLGDEGMVTFFIVAKQKTG
                     EMLTSPDIITRGFVYIREAEQLMHQLREEVKRSYLAAQKRHSGSMEDLKAAMRDDIAK
                     FLEEETGRNPMIIPVITTV"
     gene            complement(1742..2308)
                     /locus_tag="VGE59_04090"
     CDS             complement(1742..2308)
                     /locus_tag="VGE59_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074844.1"
                     /translation="MKKAWFLPILCLISFLAGTTTTARATGSTAQADNLATANAFISS
                     LQRTNEILERTVANGNENLRQSRAELAAEKQKTNELRDMVDEFLADLEKVEQPDEAMV
                     KKLYGVTFPKEASKDPAKLQLVVRQYQSLLQQARWRLKSSLRDRRGPREFLGQSLQEF
                     DQDRAKPVVLPPALYEGLPKARPDAPNG"
     gene            2442..4508
                     /locus_tag="VGE59_04095"
     CDS             2442..4508
                     /locus_tag="VGE59_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY1074845.1"
                     /translation="MPFLNRRRRSASRSRRKPTFKDQLDWTINPDTAREVFGILLTVL
                     GILFVISIFGLAGKFGEVVIRISKSLFGILGYVAPLVFLVMGIKLLMPRKDDEGVKAS
                     VAIGIIIAFFTIPGLFIQYGGAMGNLSFKLASSILGTLGGYIALLGFSIAALLVATNV
                     SLAYIVARLKFNQESMPQLNLGGGAKAKVFTLPRSERRTKEASTSAPVRVLTSASGDW
                     EYPSLDLLELSDSMPDAGNIGKNRETIQKTFKDFNIDVTMGDVNVGPTVSQYTLKPAD
                     GVKLTAISARDKDLALSLAASTVRIEAPIPGKSAVGIEIPNKSAAKVTLREILESDSL
                     KKVQSGLALALGRDSAGDPFVIDLKRAPHMLIAGSTGSGKSVCINSILVNLLYQNSPS
                     ELRLLLVDPKRVEFTEYNGIPHLLAPVVTEVDKTVALLKWTVAEMERRFKLFAESSAR
                     NIEVYNENPTEGKMPYIVVVIDELADLMTQAASDVETSIVRIAQMARAVGIHLIVATQ
                     RPSVDVITGLIKANIPTRIAFAVASNADSRTILDQGGAEKLLGRGDMLYLSSDIPNAK
                     RAQGVMVTDKEIRSVTDFLKREGEAEYDESITNFKARNPNSIGIGGDGEIDDTLFQEA
                     KETVIQSGKASASLLQRRLRVGYARAARLLDLLEEQGVIGPADGAKPRDILVSSTEDF
                     TSSEML"
     gene            4567..5283
                     /locus_tag="VGE59_04100"
     CDS             4567..5283
                     /locus_tag="VGE59_04100"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074846.1"
                     /translation="MAAHGFQQKTVTPAESLGVRLKSLRMKKKLTLSQAEQETKVRVH
                     YLEAIEDDRFKDLPSSHCKGFIRRYAAYLGLSSEVTEQSLATLYLDISRHPFSPRMAG
                     RESLVSVTPKLLAVGFSIVVLLAFIGYVTYQVRQFASPPSLEITSPNNDQVVESETVR
                     IEGKTEPGALVMIDDIQASVGSDGVFSYVLILRPGLNQITVVSENRIKKQTSRIVSIL
                     YRAPVSVSPVPSVSPSPSVQ"
     gene            5302..6420
                     /gene="recA"
                     /locus_tag="VGE59_04105"
     CDS             5302..6420
                     /gene="recA"
                     /locus_tag="VGE59_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY1074847.1"
                     /translation="MAKKSKDPSPEISVPHEGGDTSARNQALEIAIGAIQKQFGQGAI
                     MRMGGGRSHHIASIPTGSLALDVALGIGGLPRGRIVEIYGPESSGKTTLTLSCIAQTQ
                     KKGGLAAFVDAEHAFDPEYARKLGVNLDELLISQPDTGEQALEIVETLVRSNALDIVV
                     VDSVAALVPRSELEGDIGDASVGVQARLMSQALRKITGAISKSKTIVVFINQIRMKIG
                     VMFGNPETTSGGNALKFYASVRMDIRRGEQIKEGDVAVGNRTKVKIVKNKVAPPFRVA
                     EFDLLYAEGISREGDILDLAVGKDIVQRSGSWYQYEGEKIGQGRESARTFLKNNPSIA
                     DEIEAKIRKGDDATPEEVPAAEIAVEDEDSAELIENEV"
     gene            6498..7577
                     /locus_tag="VGE59_04110"
     CDS             6498..7577
                     /locus_tag="VGE59_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY1074848.1"
                     /translation="MNPVLIRRLHMPSLGVLALLIGGILRVIAAVFHWPIGLVLVSQL
                     TLLLAVLWILFYDIFPLFLNPFYLIRKRFPRRHSFFVLLFLLFGLMQLVTVFLPSGRT
                     KDFTTTLSFLSFMGGVAWFVCVTILGAFTPLIRHWKYQPRESEKVTFEDIVTSMALIV
                     TPTVALSFFFSPPGLGTTEVTPYQIFVTSLLTNIFMGIYLYLWIIRPRVFTLKQLGLK
                     KVPRERVGEAFVLFVFIVVLIAILQAVLKRLGLPLQNYSFSTKEGAAWAMIMVAVVSP
                     FVEELYFRGFLFKGLMLNHRPQIAYLVSAGLFALLHPPLAAMVEVFAIGILLGYLSKE
                     TKSIWPGIFIHALNNAIVFGYLLYK"
     gene            complement(7568..8164)
                     /locus_tag="VGE59_04115"
     CDS             complement(7568..8164)
                     /locus_tag="VGE59_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074849.1"
                     /translation="MPKQELTRVFNKILIQSKEYQEAITLLEKNHVGKTWIIGGFIYR
                     NLASCLYGTRPVTSDMDFIVEQIPTKPSLPDSWNHTVNHFGNPKYSRDGLSLDVVPLT
                     NIHSIRTNKLAPTIENYLLGVPLTIQSIAYDIETKEIIGDVGITSLLQKVVSVNNSEE
                     LHYLAQKYKRSMNSYISEKASSLCFTSKLISSEPHTYL"
     gene            complement(8189..10900)
                     /gene="polA"
                     /locus_tag="VGE59_04120"
     CDS             complement(8189..10900)
                     /gene="polA"
                     /locus_tag="VGE59_04120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY1074850.1"
                     /translation="MTPPKDALLLIDGHAVLYRAFHALPPLQTKDGLQTNAIYGFITT
                     LLKAIEEVKPKYCAVAFDLSGKTFRHDAFEGYKASRKETPDGLKTQIPYAYKVAQALN
                     IPIFTKETFEADDIIGTLAKQANAQGIPVVIVTGDKDSFQLVNDLTFVYTMKRSITDT
                     MLYDAAGVKERMGVHPSQVVDYKALAGDTSDEIPGITGIGDKTAINLLEFAGSLDALY
                     ELLKTNKDVPVTPRIREKLLEGEKIAYQSQSLATIHRDVPITLELKACDIADYDPQKV
                     SDLFNALEFYSLIKRLPKAHALEVVTTQNERDWTYRTLVSEAGISAVVLELQKAEALT
                     VDTETDGVDGPIIGISLAGQESLGYYIPLIPEHGATLSKEKIATLLKKLLEDTNVAKV
                     GHNIKYDMCSLEQLGINLSPIGFDTMVAAYILHAHLRTFDFDTVCQREFGYTPISFTD
                     VAGSKKKDATLVTAPADKVAEYAAEDAILTHRLYLHYAKQLQKEKHLKTVAETIDFPL
                     IPVLKAMEQHGVCLDINQLKVLEEKLEKEIETIRTTIESYANGPININSPAQLQKLLF
                     EDLKLDKAGMKATQTGISTAASELEKLQGMHPVIDDIMRYRELTKLQSTYVKTLPELV
                     DTDGRLHTSFSITTAATGRLSSSDPNLQNIPIRSELGNEIRKAFVAPKGYDLVSLDYS
                     QMELRIIAHLSQDPTLLQVYKDGRDIHDEVSKQLGVDRRAAKAINFGIIYGLSAFGLA
                     QGLKIEQKVAKQYIDAYFATYKGVADYLAKTKQEIRDKGYVETIFGRRREIPEIKAAN
                     AMVRAAAERMAINAPAQGTEADIIKLAMIQVHDWIEHTYQGAKDRPYLVLQVHDSLLL
                     ESPESETERVAAKVKELMEHAYELDIPLTVDCAVGKNWGELEKQQTA"
     gene            11008..12816
                     /gene="typA"
                     /locus_tag="VGE59_04125"
     CDS             11008..12816
                     /gene="typA"
                     /locus_tag="VGE59_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY1074851.1"
                     /translation="MNIRNVAIIAHVDHGKTTLVDGLLKQAHTFRENEAAMSQTLIMD
                     SNDQERERGITILAKNTAVVYKDTKINIIDTPGHADFGGEVERTLNMADGAVLVVDAQ
                     EGPMPQTKFVLKKALEIGLRPIVVLNKIDKRDARPDVVIGKVYDLFLDLVTDEAQLEF
                     PVYYAIGRDGKAWAELPANPDEDADLSPLFEAILEHVPAPEGESNAPFQMLVTTLDRD
                     NYQGKYAIGRIRRGIAKPGLPVVLMGSDGKQEQGRIDKVFVSQGLKRVEVPSAEAGDV
                     VALTGIKGANIGETIADATQPEALPTIAIEEPTLRMSLSANTSPFAGKEGQYVTSRHI
                     LERIQKELETNVAMRLEVKDGGEYILSGRGELHLSVFIETLRREGYELQVGKPQVITR
                     EIEGVMKEPIEELTIDVTDEYVGAVTSEVGRRKGILLSQSENGDGSTRLIFEIITRGL
                     LGLRNQLLTLSRGTAVMNSLFLRWEPVGAAIPKLRNGVLIASEAGKAMTYGIENAQER
                     GIVFVGPQTPVYEGMIIGLNQRENDLEINICKEKKLTNVRSNAEISATITPPLNLSLE
                     QSLDFLEDDELLEVTPKNLRLRKRVLNATQRARSRK"
     gene            complement(12853..15489)
                     /locus_tag="VGE59_04130"
     CDS             complement(12853..15489)
                     /locus_tag="VGE59_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074852.1"
                     /translation="MKHITARLSILLLITVLFGTGLIRSQYTHAQSPTGQAPKLFWSP
                     ERQAVLNNLKNDPRWFSVIKSKADAAIPSDAAVQQCFACYGTWPTLMYQMTGDTAYAQ
                     KAWQGISPVVSSNPPSLPADGSNGTRQSLATYALIYDWLKPALSAEQRQTFRTSLIYW
                     SKLTIGEIPGTNFASGRLDDSDEVVGHYFGVIMTALTIEDEDPATSTFLINHPHVGGF
                     DATSNSLDNKTSTMRNAIKRYAEMAQGGEWIESSDYNVNTVMLMAIGYMGVKTATETD
                     HFPELASYFKEAAIQSMHILTPDLKQNYQWGDEQHPRDIRLYGKLPHMAALAAVNESD
                     TTVAPLVWGAIKTLMDTYPNDTFVKTPPFTFFLFFNPAATPATDWQTKLARSHTTTNQ
                     GLTHFRTGWGNAASFFTSSFLNANAYVDHQQMITGDFQLYRKGEWAITHPIGYTLSSL
                     ASNVMRFSGYSATQEARGITAQEVDPNGTYVYTSGSAGGTIHRSGYWNPPATFLHEWT
                     RSFLYLPSQDSSRDTVVIFDRTLADDPKQLYGWAENRYEGGARSTIENSGGRKEWILH
                     TSEAPIVTGNQASWRTKNGQFIQLTNLLPTNTQKTIVNEKAPAQKFNGFIYDYEQAFH
                     IAIKPTIDQQWDTFLSVVTAGEEANPATPTLIESADKTVQGVALEQGTQTTLSFFSAK
                     PGKPVPTLKNGDVLTYDASLFSKIKDTRLIREAFQITTTTSHTSNLFIADLDPAQRWS
                     LKINNTRIPFSVSEKGLGRATIETTGALSLAVIPNDIEPEGKASLEVTVAADSVQAKT
                     GETLRYQVTYRNNGEKAASQAKFTTAIPSEFEVIEASLPTTPAVLQDTSTNTLTWTIP
                     ALAAGETGALSFQVKVKPRSSK"
     gene            complement(15486..16340)
                     /locus_tag="VGE59_04135"
     CDS             complement(15486..16340)
                     /locus_tag="VGE59_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074853.1"
                     /translation="MMHKSLIALFIASFMLWPLGDAQAADKTAILKLSVPTSTVTQGN
                     QVTVSLSLLPGGQAVIGTQVTLYFSSTLTYQSFDGAGSIFDTEIDVPTVNGNTLSFSR
                     VAMNTSYVGTEGLIGKITFVANEPGAGDITIQEATTQVKADSDLTNILQSTQSAHTTI
                     SAPTVTTTVTPTPTPTATPTASISPAATSTPKASPKATTTSQIVVAVTASSKATPSET
                     PLTTTTPSTESSPEALIDITPTPNPIQSSVPTSAERSRARSFAIALVVLGACGTIVSL
                     LYLRGSHV"
     gene            complement(16330..17067)
                     /locus_tag="VGE59_04140"
     CDS             complement(16330..17067)
                     /locus_tag="VGE59_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074854.1"
                     /translation="MRHTNHRLSFWHHKTLSIVLLSLLLTGLYFLFQKKSLTPSASTE
                     KRASSQVSVAFCTEGEGTDTCDPSQNLYSEHAISNVLSFTVTQNDPPLQAICLQAETE
                     SLLVTPLHGTAIISDTKGTTLATYQDLTSDKDGKITIALEETPALTLPITLRFKADGY
                     FARSITVENYDANTCINYAQANKLIRGDFNNDGALTFDDVTLIIKAYRGTSLQFVPAV
                     FGEEKPEFSHVLEIIKKYRQQKDFHDA"
     gene            complement(17126..17935)
                     /locus_tag="VGE59_04145"
     CDS             complement(17126..17935)
                     /locus_tag="VGE59_04145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1074855.1"
                     /translation="MIKTALITGASQGIGAAFARLLGEQGYNLILLSQNKVLLEALAR
                     EIERDWGVTVSIEILDLSDTLQRRKTIEKILDHHTVDVLINNAGFGLSGSFIDTSLDY
                     ELGMIEVHVAATTEVTKLVLKQMKGHHKGHIINVASTAAFQPGPFMAVYYATKAYILS
                     FSEALAEELKKSGISVTALCPGPTKTGFATRAHMLQTNLFTRHRLMSAELVALIGYRG
                     MLNGKRVVVPGTLNTIGSLATRIVPRPWVFRIMRGVHSRSPRRTAKNAPHS"
     gene            18041..18742
                     /locus_tag="VGE59_04150"
     CDS             18041..18742
                     /locus_tag="VGE59_04150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1074856.1"
                     /translation="MKKVLITGGNKGIGLALTELFLENGYAVAVIARDFTHFSYKEDK
                     RVQCFAHDLTDIEAIPQLVREVGEVDVLINNAGIMFSLPHDEYPSDKKEYILKLNLKA
                     PIALIEAVSRLMVTKGDGRIVNVASIAGEIGHPDIWYGVTKAALINATKSFARLLGPD
                     GIVVNCVAPGPVTDTEMFEVVPQARKEQLQKSTITQTFATPVDVAQTVFWLADSSPSY
                     INGVCIDINNGSFPR"
     gene            complement(18746..19183)
                     /locus_tag="VGE59_04155"
     CDS             complement(18746..19183)
                     /locus_tag="VGE59_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1074857.1"
                     /translation="MARYIDSTGTKIALLHEKKIVVLLRDNIPELPYANMWDLPGGGK
                     EGNETPRQCLAREVNEELGIAVNENSIIWEKLYTEPEDTTLHFSFMVCFINQATIDKI
                     VLGDEGQRWMLMPISKFFEDAQVIGKFKPPLRDFLSQSHYSYV"
     gene            complement(19186..20523)
                     /locus_tag="VGE59_04160"
     CDS             complement(19186..20523)
                     /locus_tag="VGE59_04160"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY1074858.1"
                     /translation="MKKILKSILRNRISPTTIKSLRKIESTLMASQYGFPAKKLRIIG
                     VTGTNGKTSTCHYITQLLEESGHKVGMLTTVAISINGKRELNKTKKTTLDTRELHMHL
                     KKFVDAGCEYAVVETTSHALDQNRVYGIYYEAVGFTNLTHEHLDYHKTMGEYQKAKEK
                     LFANNPKVSVVNADDPAAKTFLSYPASKKFTYGILDPLRPKEALKDPDITASWLKMGS
                     TTTSFKLVTPYGETEVSMPLPGRFTVANVLCAVGITLGLGIPFQEITKSIRKLRPVEG
                     RMEPVDLGQPYSIIVDFAHTPDALQQVYSTLRPMVKGRLIAVLGSMGDRDKTKRPILG
                     ALAARYADFVIVTNEDPATEDPASIINEVAGGIPRGRPNAEEAKKGENHWWWKIPDRR
                     EAISKAIGMAEKGDTVLITGKGGEHVMLIGEKLIPWNDVKVVREILEQMNSQK"
     gene            complement(20526..21476)
                     /locus_tag="VGE59_04165"
     CDS             complement(20526..21476)
                     /locus_tag="VGE59_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEY1074859.1"
                     /translation="MQNKTPYPKSFLQSDIWGSFRETQGWHAHKLEDVLILERPLPLG
                     KSFLYAPEVFHHPSLLITLLPAIYEVAQRRNSLFFRLELNIPKTDPLAQQWRAAFTYT
                     GFQKAFESVQPDDRQIVTLSGGEHATLKQMKQKGRYNIRVAERAGVTVRETTLKTLEE
                     DVAIFYALFEETAKRDKFSIRSQAYFQELAQVLYHHNCGKVFIASLQGIPLAASITTL
                     YDGVASYLYGASSSEHRAAMAPYLLHWTVMQWAMSKNATSYDLLAIKPDTDKKHAYDG
                     ITRFKQQFGGESIHLMGSWDVPLQALWYTLFKAAESIRRH"
     gene            21525..22583
                     /gene="tsaD"
                     /locus_tag="VGE59_04170"
     CDS             21525..22583
                     /gene="tsaD"
                     /locus_tag="VGE59_04170"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY1074860.1"
                     /translation="MRILAIETSCDETAAAVMERTDTSPWVKTITSVVASQAKLHEKY
                     GGVYPEVASREHIRKILPVIAEALDLPLHERGRLPNFSAAGIDYLAVTVGPGLIGSLL
                     VGTQTIASLGYATGLPILPVNHLAGHIYASFIATESVLTAPDFPLLSLVVSGGHTMLI
                     YMKGHHQYELIGQTRDDAAGEAFDKVAKLLGLGYPGGPLVSQLARKGNRHAYKFPIGL
                     EHEDTCDFSFSGLKTAVLREVQKAGALSEQGKADIAASFERAAIEALLLKTSRALQSR
                     PVRHFILGGGVAANNYLRERLATLLAHKTPAVTLHVPPSHLCTDNAEVIGAAAAFSNL
                     VPIAPSELKTYARYPLTS"
     gene            complement(22594..23205)
                     /locus_tag="VGE59_04175"
     CDS             complement(22594..23205)
                     /locus_tag="VGE59_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074861.1"
                     /translation="MINLIKKLKKSFQLLPRTRSALLSLSIILAAFLVSGGLLMYDYT
                     ASHGKAAEPQPTATATPEPWKTYTNEAFHFSIQYPADWKIGFRDSTQIHFFPPEAPDF
                     NPDSDGQGPIVVFIYPKNSTGLAPKEYKQRLFLRVDGLEAVTYSDIPAGYENVQTHLI
                     TDTYINHPEAILGVTFFNEVSTPADDDLLIVYQAMLNTLKQAQ"
     gene            23291..25417
                     /locus_tag="VGE59_04180"
     CDS             23291..25417
                     /locus_tag="VGE59_04180"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY1074862.1"
                     /translation="MQDFPKRFDSATAEPLIYDKWVASGGFSPSMESQAEARSILMPP
                     PNANGALHLGHAYEIAIQDTLIRYWRMRGYKTLWQPGADHAGFETQYVFDRKLEKEGR
                     NRFSIPRAELYQEIYDFSIANQAVIRQQFERMGASADWSRFRFMLEPDLVKTVYKTFK
                     QLYDDGLIYRAKRPVHWCIKDQTTLSDLEVTDQEQTDKLYYLTYGPVTVATVRPETQF
                     GDVAIAVHPEDERYKDLVGTTVDVKMAHQTVSLPVIADDYVNREFGTGALKITPAHDP
                     NDFEIAQRHNLDLPIVIDQYGKLTDQCGQFSGMKIADARAAVVAELESLGVVEKIEAY
                     PHTVKVCYKCNRLIEPRIIDQWWLSLTKPSQSKQKSLRDLAVGAVASGETRFITERYE
                     NQFHSWMESVRDWPLSRQIVWGIQLPVWYAKDGSVVVTEGEEPANASELTRDSDVFDT
                     WFSSCQWPFSTLGNHNDDLETFYPTTAMTPGYELIFFWVARMMMLGIYTQGKSPYKVV
                     MLHGIVRDGKGQKMSKSKGNTVDPLATADQYGADAIRLALLYGTAPGTDPTASEEKIR
                     GMRNFGTKVWNIARYITMSKSADAKSEFIPVTDADHTVIKQLHSTIATVTQALDDYQL
                     HIALESLYQFIWRDVADVYLEKTKDQLQNETTKLTTEGNLVYVLDTILRLLHPFAPFV
                     TEALWSEVMPGQPLLLVSQWPEVTNG"
     gene            25410..25967
                     /locus_tag="VGE59_04185"
     CDS             25410..25967
                     /locus_tag="VGE59_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074863.1"
                     /translation="MVEFQVPPTYRVATRLASNVSVAPRLSSRFAIILGSVFFGGLIV
                     IKLIAGAIYAEETRLLPSAETEILPTYQLTVKKMTNVEKAMKSADRLVSKGYYDFSEL
                     LYAQASTLDPNLRDASYGWAYSIIKKPHLASKDLVDLHTAMSRAEAVDPLYPPLLQLK
                     LIVATLEKDQSTTTATQARLDSLKF"
     gene            26085..26492
                     /locus_tag="VGE59_04190"
     CDS             26085..26492
                     /locus_tag="VGE59_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074864.1"
                     /translation="MVRRLFMLALVAGIVSLGAVAPVRSQEKAAPVKRDPRLIAKWEL
                     SNGAFFRFWDDGEAEKGYGPILKHGSWRTEKTSTGKDQLICVWRDDAVFPNSEGVYTL
                     SSDGTILTLDIVEGRDLKKETPQRVKLEKVVRK"
     gene            26611..27768
                     /locus_tag="VGE59_04195"
     CDS             26611..27768
                     /locus_tag="VGE59_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074865.1"
                     /translation="MLKGWGSYFSVCGLVMAFFISLFSPVHVVAAGESLLGLTATLVP
                     SGSTSQLVVSFRIQNYQWQSNKRFRVSVSTDNRTDKEVTFKAGKGGPNYSQTNYTVYI
                     AGYRPVAGGAGVIKVTLSDQGVISHSPITSSTTRVTTPTPPKFQEVSVYSDYSSAAGK
                     NVIWVKSTILPDSYRSGLTLRVVNKETNAQYIEANNITQVYANATNVDAPSTAQQVRF
                     TLYEGATVLDTKDATLGSIDSQVEITVGSTPQGGLVKVFLDGVDKGTTPVTLRPDKDT
                     DSEPAQHKIKITNGTVEKEFDVTFEANHTLYYNFTTEGGGVDADPTEADKPKTTTAGA
                     AGQSKPCNEYLGFSFGSGLQFNIPAYILCQFNSLIQLIAKTTQGVIDGLPL"
     gene            27752..>29752
                     /locus_tag="VGE59_04200"
     CDS             27752..>29752
                     /locus_tag="VGE59_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074866.1"
                     /translation="MVSRSRWLLSGLLLIVLGVLFFPASTQAALAPGTDHRVWTWVEG
                     TNGVINVYKVVLGLVDFVVVLGLLAAAFANIFSFIPIKLDSYKVKQVLPGLIIGIVMA
                     NLSYFIMHLFIEVSTIIVQMIGIIVSQQVGDTIGTQSGTGYIITKLWQEILGSLFKLP
                     TAWAGLSGVVTAIAGGGGLIAAGATLTEMLAAIGGSLILNPAGGAIGIMFGLVLLAPL
                     IIALILAFLLYIRGYILIICFVLSPLAFFSLGFPPLKTFWNKWWTNFWKWLIMAPLVF
                     LVLAGGIIFIHFANNAAGGSRNLTDYIFFNGVGIGFLAVAMKLPFSFGTFFGANVMKE
                     YNNWSKRAAKTGWDGGVRGTAYIGGAQKALRGHAGSWTNRADLKDKFAAANAAGKAAM
                     TTDKITKGWLPNNPIRLAEGVRAGMKARLETADKADAKMIKKTVTYRGAASREAKYRD
                     YTESNGHIKEETDPRTLVDDHIIKNGGDKFTPEELNKLLTESPGELVELPIVRRAEQE
                     GVDLEKLAKLMIAWKQYKSISGRKNIKSGVLDPPYPGRGSIGDPDYVPPRDAGRGYNA
                     LVRDAGTGRITGFNQQRFIQGNSDEDVMGAHVGPNHSDDGNSSSEDGNNNPGGNKPAA
                     PKGPQGGGSGGNQSPQRGVEQGDSSHPLLSQSLDTATPSVNIP"
BASE COUNT         7336 a         6394 c         7514 g         8508 t
ORIGIN      
        1 cgaagatatc gttgttgtag actgcgggat tgcctttgct ggtcctgaat accctggcat
       61 tgatgcatta attccagatg tccgttactt agaagagcga aaaaataata ttcgtggcat
      121 gatcttcacc catggacatg aagaccatat tggtgccgtt ccttacattt ggcccaagct
      181 tggttgcccc atgtatgcaa ctccgctagc cggtggcctc attcaacaca agctcactga
      241 agcgggtatt accaacgcgc caatgacggt agttcaggca ggtgataaat tacactttgg
      301 aaaaatttca gtcgaaatgg tgcgtctcac gcactcgatc cctgatgtcc ttggtcttgc
      361 aatccgttgt cccgctggtc ttttcttcta cgcgacggac tggcgtttag agcacacacc
      421 tgcgctcggt aagccagccg attggacgcg tatcgcacag cttgcaggcg aaggtattac
      481 ggctctcttc tcagactcga ccaatgttga tcgccctggg tatagcccaa cggagcgaat
      541 tattggtgaa gcctttgacc gcatcttcca agatgccaaa gcgcgcatta ttattgctca
      601 gtttgcgtcc aatattaacc gcgtgcagca agtttttaac tcggctaaaa agtttggccg
      661 taagattgcg ctgtctggtc gctcaatgga aacgtacgtc aatattgcga tgggacttgg
      721 gtatcttaag gttcctgagg gcatgttgat tgatcttcgt aaggtgaata cctttgatcc
      781 gcataaagtt gccattctct ccacgggcag tcaaggcgaa gagttttcgt cgctcgtccg
      841 gatgtctgaa ggggcacatc gtcacgttaa gcttcatccc acggatacag ttgtcgtttc
      901 tgcttctgct atccctggca acgaagatca ggtagaacgg ctcttaacga acctctatcg
      961 tgaaggcgtg aacgtaatca acaacaagct tgtcgctgat gtccatgtgt caggtcaccc
     1021 ttcgcgggat gaactgaaaa tgatgattgc cctggcgcag ccgcagcact ttattccaat
     1081 tcacggttca tatcctcgta tggtcgacca tggtaatatc gccgtcgaaa tgggtgtaga
     1141 accagacaaa attatcatga tggaaaatgg ccagatcgtt gagttcgacg aaaagggcgg
     1201 ccgtatttct gccgaacgta ttccaatgga agacgtgatg attgacggta agaaaatcgg
     1261 cgatgttggt ccgattgtca ttcgtgaccg taagacactt ggcgatgaag gtatggttac
     1321 tttctttatt gtggccaagc agaaaacggg cgaaatgctc acatctcctg acattattac
     1381 tcgtgggttt gtgtatattc gtgaagctga acagctcatg caccagttgc gtgaagaagt
     1441 aaaacgctcg tatctcgccg cgcagaaacg tcacagcgga agcatggaag atctcaaagc
     1501 tgctatgcgt gatgatatcg cgaaattctt agaagaagag acaggccgta atccaatgat
     1561 tatccctgtc atcacgacgg tctaaaagtg aaagaataaa actaagaaaa cgtcgccact
     1621 aaaccaggcg acgttttctt ataatctcgc tacgcggtaa taaaaagggg cgacgtcgta
     1681 cgacgtcgcc ccggctcgat cagggttgtt tttctctcgt tgcttcctgt gaaggaagat
     1741 gttacccgtt gggggcatcc ggtcgggctt tgggcaagcc ttcgtagagg gcgggaggca
     1801 ggacgaccgg ctttgcgcga tcttggtcaa attcctggag tgattgcccc agaaactccc
     1861 gtggccctcg tcggtctcgc agtgaacttt tgagtcgcca ccgcgcttgt tggagcaggc
     1921 tttgatactg ccttacaacg agctggagtt tggcagggtc tttcgaggcc tctttgggga
     1981 aagtgacgcc gtaaagcttt tttaccatgg cttcgtcggg ctgttcgact ttctcgaggt
     2041 ctgcgaggaa ctcgtctacc atgtcccgta gttcgttggt tttctgcttt tcggcggcta
     2101 gctctgcccg cgactgacgc aagttctcgt taccgtttgc gactgttctc tcgaggatct
     2161 catttgtcct ctgaagtgaa ctgataaatg cgttggcagt cgcaaggttg tcggcttggg
     2221 cggtactccc cgttgcccgt gcggttgtgg ttgttccggc aaggaacgag atcagacaca
     2281 aaatcgggag aaaccaggct tttttcatcg ggcttcctca gagatcagga acggagatag
     2341 cgagaatata ccgtagtaag gggagcttgt caaccgaaaa gctctgtggc aatgggttag
     2401 gaaacctgct agactgggtg tgttaccgaa ggaggtgtgt tttgccgttt cttaatcgcc
     2461 gccgacgctc cgcgtctcgg tctcgccgga agccgacgtt taaagaccag cttgattgga
     2521 caattaaccc tgacactgcc cgggaagtgt ttgggattct tttaactgtt ttaggcattc
     2581 tctttgtgat ttcgatcttt ggtttagcgg gtaaatttgg cgaagttgtg attcgtattt
     2641 ccaaatcgct ctttggcata ttagggtatg tggcccccct tgtttttctc gtcatgggaa
     2701 taaagttgct tatgccgcgt aaagatgacg aaggagtaaa agcatcggtt gctatcggca
     2761 ttattattgc cttttttact atcccaggcc tgtttatcca gtatggtgga gctatgggaa
     2821 atctctcttt taaactcgct tccagcattc tcggaacgct cggtggttat attgccttac
     2881 tgggcttttc gatcgcggct cttttagtgg ccacgaatgt gtcgctggcc tatattgtgg
     2941 cgcggcttaa atttaatcaa gaatcaatgc cacaacttaa tctcggagga ggtgctaagg
     3001 cgaaagtatt tacactgcct cgttctgaac gccgtacgaa ggaagcttca acctctgctc
     3061 ctgttcgggt gcttacgtca gcctcggggg attgggagta tccgtcgctt gatctacttg
     3121 agctctctga ctcaatgccc gatgccggta acattgggaa gaaccgcgaa acaattcaga
     3181 agacattcaa agactttaat attgacgtca cgatgggtga cgttaatgtt ggtccaactg
     3241 tctcgcaata cacattaaaa ccagctgatg gagttaaact cacggctata tctgcccgag
     3301 ataaggactt agccttgtct ttagccgctt caacagtccg tattgaagcg cctattcccg
     3361 gaaaatctgc cgtcggaatt gaaattccta ataagtcagc cgccaaagtt acgctgcgtg
     3421 aaattttaga aagtgattca cttaaaaaag ttcagtccgg tttggccttg gcgcttgggc
     3481 gagattccgc cggcgacccg tttgttatcg atctcaagcg tgcgccgcac atgcttattg
     3541 caggttcgac cggaagtggt aagtcggtgt gtattaactc gattttagta aacttactgt
     3601 accagaattc ccccagtgag ctacgcctgt tgcttgttga tccgaaacgg gtggaattta
     3661 cggaatataa cgggattccc catctcctcg ctccagttgt gacagaagta gacaagacgg
     3721 tggccttgtt gaagtggacg gtcgcagaaa tggaacgacg gtttaagctg tttgccgagt
     3781 cctcggcccg taatattgaa gtctataacg aaaacccgac agaagggaaa atgccctata
     3841 ttgtcgttgt tatcgacgag ttggccgacc ttatgacgca agccgccagt gacgttgaaa
     3901 cgtcgattgt gcgtattgcg cagatggctc gtgcggtggg tattcactta attgtcgcca
     3961 cccaacgtcc ctcggttgac gttatcacgg gtttaatcaa ggccaatatt cccacgcgta
     4021 ttgcgtttgc tgtggccagt aacgccgatt cccgtacgat tctagaccaa ggtggggcag
     4081 aaaaactatt aggccggggc gatatgcttt atctctcaag tgatattcca aatgccaaac
     4141 gtgcgcaagg tgtgatggta actgataaag aaattcgttc cgttactgac ttcctcaagc
     4201 gggaaggcga agctgagtat gatgaatcga tcacgaactt taaggcccgt aatcccaact
     4261 caattgggat tggtggtgat ggagaaattg acgatactct tttccaagaa gccaaagaaa
     4321 ccgtcattca atcaggtaag gcgtcagcgt ctcttctgca gcgccgtttg cgggttgggt
     4381 atgcccgagc cgctcgctta cttgatctgt tagaagaaca aggggtaatt gggccggctg
     4441 atggtgccaa gccccgcgat attttagtct cctcaacgga agactttacc tccagcgaga
     4501 tgttatagct ttggagtctg tgttaccaga gctgtaaaag ttagtcacct gtgttatagc
     4561 tagaatatgg ccgcccatgg atttcaacaa aaaacagtaa caccagccga aagtctgggt
     4621 gttcgtttga agtcgctccg gatgaaaaag aaactcacgt tgtctcaggc cgaacaagag
     4681 acgaaagtcc gtgtccacta ccttgaggcg attgaggatg accgatttaa agatcttccg
     4741 tcctcgcact gtaagggctt tatccggcgg tacgcagcat atttggggct ctcttccgag
     4801 gtaacggaac agtcacttgc cacgttatac cttgatatct ctcgacatcc gttctctccc
     4861 cgaatggctg gccgcgagtc gctggtatcg gttactccaa agcttctggc ggtcggtttc
     4921 tcgattgttg tattgctcgc ctttattggg tatgttacat accaggtacg gcagttcgct
     4981 tctcctccgt cgcttgagat taccagcccg aataatgacc aagtggtgga atcggaaact
     5041 gtacggattg agggaaaaac tgagcctggc gcactcgtga tgatcgacga tatacaagcg
     5101 agtgtgggga gcgacggtgt gttttcatac gtcttaatac tacgccctgg tcttaaccag
     5161 attaccgttg tctcggaaaa tcgcattaaa aagcaaacgt cacgtattgt ctcgattctc
     5221 tatcgagcgc ctgtttctgt gagtcccgtt ccaagtgttt cgccgtcccc tagtgttcag
     5281 taagtgtgtt aaaggagttt tatggctaaa aaatcaaaag atccgtctcc cgaaattagt
     5341 gtgccacacg aaggtggtga tacatcggct cgtaatcagg cgctcgaaat tgccattggc
     5401 gcaatccaaa agcagtttgg gcaaggtgcc attatgcgta tgggtggcgg tcggtcgcat
     5461 catattgcct ctattcccac gggttctctg gctctcgatg ttgccttagg cattggtggt
     5521 ttgccacgag gccgtatcgt tgaaatttat ggtcctgaat cgtcagggaa aacaacgctc
     5581 acattgtcct gtattgcaca gactcaaaaa aaaggcggct tagccgcatt tgtagatgcc
     5641 gagcatgcct ttgacccaga atatgcccgt aagttaggcg ttaaccttga cgaactcttg
     5701 atctctcagc cggatacggg tgagcaagcg ctggagattg tggaaacact tgtacgatcg
     5761 aatgccctgg atattgtggt tgttgactcc gtcgcggctt tggttccccg ttcagagtta
     5821 gaaggagata ttggggacgc atcggttggt gtgcaggccc gtttaatgag ccaggccttg
     5881 cgtaaaatta ccggtgctat ttctaagtcc aaaacgattg tggtcttcat taaccaaatt
     5941 cgtatgaaaa ttggagtgat gtttggaaac ccagaaacta cttctggtgg taatgcgctt
     6001 aaattctatg cctcggtgcg aatggatatt cgtcgcggtg agcaaattaa agaaggcgac
     6061 gtggccgtgg gaaaccggac aaaggtgaag atcgtgaaaa ataaagtggc gccaccgttt
     6121 cgagtggcgg agtttgacct tctttatgcc gagggtattt cacgtgaagg cgatatttta
     6181 gacttagcag ttggcaagga tattgtgcag cgttccggct cttggtatca gtatgaaggc
     6241 gagaagattg gccagggacg tgagtcggcc cgaaccttcc tcaaaaataa cccgtcgatt
     6301 gccgatgaga ttgaagcgaa gattcgtaaa ggcgatgatg caactcctga agaagtgccc
     6361 gcagccgaaa ttgcagtcga agatgaagac tccgctgaac ttatagaaaa cgaagtttaa
     6421 gagcgagaag taaaaagaga gcgttacaac gctctctttt ttttgcaccc aagcggtaat
     6481 tacgttagca tgaatatatg aatcctgtgc ttattcgtcg acttcatatg ccctcgcttg
     6541 gtgtcttagc tttactcatt ggaggcatcc tacgagttat agcggccgtg ttccattggc
     6601 ccatcggcct tgttttggtt tctcagctga cactgctttt ggctgtactc tggattctct
     6661 tttacgatat ttttcctctc tttttgaacc cattttactt gattcgtaag cgattccccc
     6721 gtcgtcactc ttttttcgtt ctccttttcc tcctatttgg actaatgcaa ctggtgactg
     6781 ttttcttgcc atcgggacga accaaagatt ttaccacaac cctctcattt ttaagcttta
     6841 tgggtggcgt ggcgtggttt gtgtgtgtaa cgatcctcgg tgcgtttact cctctgattc
     6901 gacattggaa ataccagccg cgtgaatcag agaaagttac gttcgaagat attgtgacga
     6961 gtatggcctt aattgtgacc cccacagtgg cactctcgtt cttcttctcc cctcctggtt
     7021 tagggacgac ggaggtcact ccctaccaaa tctttgtcac tagtttgctg actaatattt
     7081 ttatggggat atatctctac ttatggatta ttcgtccccg agtatttacc cttaagcagc
     7141 tagggttaaa gaaagttccg agagagagag taggagaagc attcgtcctg tttgtcttta
     7201 ttgttgtcct tattgctatc ttgcaagcag tgctcaagcg gcttggattg ccccttcaga
     7261 actatagttt tagtacgaaa gagggtgcag cgtgggctat gattatggtt gccgttgttt
     7321 caccctttgt cgaagagctg tacttccgtg gcttcttatt taaagggctc atgctcaatc
     7381 atcgcccgca gattgcctat cttgtctcgg cagggttatt tgcgttacta catccccctt
     7441 tagcagcgat ggtcgaagtc tttgcgattg gtattctttt aggctatctt tccaaggaaa
     7501 caaaaagtat ttggccgggc atctttatcc acgctttaaa taacgcgatc gtttttggtt
     7561 acttgctcta taaataggta tgaggttccg aactaatcag tttacttgtg aaacaaagtg
     7621 agcttgcttt ctcggaaata tagctattca tgctccgttt gtatttctgg gcaagatagt
     7681 gtagctcttc gctattgttt acgcttacta ccttctgtaa caacgaagtt atccctacgt
     7741 cgccaataat ctccttcgtt tctatatcgt aagcaattga ttgaatagtg agtggcaccc
     7801 cgagtaaata gttctcgatc gtaggcgcta atttgttggt gcgaatcgaa tggatgtttg
     7861 tcagtgggac aacatcgaga cttaggccgt ctcggctgta cttaggattg ccaaagtgat
     7921 tcactgtatg gttccagctg tccggtaggg aaggttttgt cgggatctgt tccacaataa
     7981 agtccatatc tgaggtaact ggtcgagttc cgtacagaca agaggccaag ttgcggtaaa
     8041 taaaccctcc gataatccac gttttgccta catggttttt ctcaagaaga gtaatcgctt
     8101 cttggtactc tttactctga attaatattt tgttgaagac gcgtgttaat tcctgtttcg
     8161 gcatggggcg tgataaagaa gcggatcttt aagcagtttg ctgtttctca agttctcccc
     8221 aattctttcc gacggcacaa tcgacagtaa gaggaatatc aagttcgtag gcatgttcca
     8281 tcagctcttt gaccttggcg gcaacgcgct cagtttcgct ttctggtgac tctaagagta
     8341 aagagtcatg gacctgaaga acgaggtaag gacgatcctt tgctccctgg taagtgtgct
     8401 cgatccagtc gtgaacttga atcatagcga gcttaattat atcggcttcc gtaccttgag
     8461 cgggtgcgtt aatcgccatg cgttcagccg cagcgcgtac catcgcgtta gcagctttaa
     8521 tttcaggaat ttcacgacgt cggccaaaga tggtttcaac gtatcctttg tcgcgaatct
     8581 cttgtttggt tttggctaag tagtctgcca cgcctttgta ggtggcaaag tatgcgtcga
     8641 tatactgctt ggcgaccttt tgctcaatct ttaatccttg cgccaatcca aaggccgaga
     8701 ggccataaat aataccgaag ttaatggctt ttgccgcacg acggtcaacg ccaagttgtt
     8761 tagaaacttc atcatgaata tctcggccat ctttatagac ttggagaaga gtgggatctt
     8821 gcgacaagtg ggcaataata cgcaattcca tttgggagta gtcgagtgat accaggtcgt
     8881 agcctttcgg agcgacgaag gctttacgga tttcgttgcc aagttcgcta cgaataggga
     8941 tgttttgtaa gttaggatcg gaggaagaga gccgccctgt tgcagcagtc gtaatactaa
     9001 aacttgtgtg tagacggcca tcggtatcga cgagctccgg aagggttttt acatatgtac
     9061 tttgaagctt cgtgagttcc cgataacgca taatatcatc aattaccgga tgcatgccct
     9121 gaagtttttc aagctcagag gcggccgttg aaatgccggt ttgcgttgct ttcatgcccg
     9181 ctttgtcgag tttcagatct tcaaacaaaa gcttttgaag ttgtgctggt gagttaatat
     9241 taattggtcc attggcatag ctctcgattg tcgtgcggat agtttcaatc tctttttcta
     9301 gtttctcttc cagtacttta agttggttaa tatctaggca tacaccgtgt tgttccatgg
     9361 ctttaaggac cggaataagg ggaaagtcaa ttgtttcggc cacagtttta agatgcttct
     9421 ctttttgaag ctgctttgca tagtgaaggt aaagccggtg tgtcagtatg gcatcttcgg
     9481 cggcgtattc cgcgacttta tctgccggtg cggttacaag tgtcgcgtct ttcttcttgc
     9541 ttccggcgac atcggtaaac gagatcggcg tatagccaaa ctcacgctga cacacagtgt
     9601 caaagtcaaa cgtacgtaag tgggcgtgga gaatataagc agcgaccatc gtatcaaagc
     9661 caatcggact gaggtttata ccgagttgtt cgagagaaca catatcatat ttgatattgt
     9721 gaccaacttt cgccacattg gtgtcttcga gaagcttctt gagaagtgtg gcaattttct
     9781 ctttggagag agttgcgccg tgttcaggaa taagaggaat gtagtagccg agtgattctt
     9841 ggcctgcgag tgagatacca ataattgggc catcaacacc gtcagtttcc gtatcgaccg
     9901 taagcgcttc ggctttttgt aattcgagaa ctactgccga tatgccagct tcactaacga
     9961 gcgttctata agtccaatca cgttcgtttt gcgtggtgac aacttccaag gcatgggctt
    10021 tcggaagccg tttgataagg gaatagaact caagcgcatt gaagagatca gaaacttttt
    10081 gtggatcata gtcagcaata tcacatgctt taagttctaa cgtaattggt acatctctat
    10141 ggatggttgc gagtgattgt gactggtagg caatcttttc accctcaagt agtttttcac
    10201 ggatacgagg cgttacaggg acgtctttat tcgtttttaa gagctcataa agtgcgtcga
    10261 gtgaaccggc aaattcgagt aggttaatag ccgttttgtc accaatgccc gtgatgccgg
    10321 gtatttcatc tgatgtatcg ccagccaatg ctttataatc aacgacttgg ctgggatgga
    10381 cccccattcg ctcctttaca ccagctgcgt catagagcat cgtgtcagta atggatcgtt
    10441 tcattgtgta cacaaaggtt aaatcgttta cgagctggaa gctgtcttta tcaccagtta
    10501 cgataaccac cggaattccc tgtgcgttgg cttgtttggc taacgttccg atgatgtcat
    10561 ctgcttcaaa ggtttctttc gtaaatatag gaatgttaag tgcttgggct actttatagg
    10621 catagggaat ttgtgttttt agtccgtcgg gagtttcctt gcggctggct ttatagcctt
    10681 cgaaggcatc gtgacgaaat gtttttccgc ttaagtcaaa agctaccgcg cagtacttcg
    10741 gttttacttc ctcaatcgct ttcaacaggg tggtaataaa accgtagatg gcgtttgtct
    10801 gcaggccatc ttttgtttgg agtggtggca gtgcatggaa cgcgcgatac agcacggcat
    10861 ggccgtcaat cagaaggagt gcatctttag gtggggtcat atatctctta tatcatattg
    10921 ttctctgtaa tacaggtttc attgctttac agaagatcaa aaatacagta gaattaacag
    10981 gttattaagc agagggaatt atttactatg aatatccgaa acgttgctat tatcgctcac
    11041 gttgaccacg gcaagacaac tcttgtagat ggtcttctta aacaggctca cacattccgc
    11101 gagaatgaag ccgctatgag tcagaccttg atcatggact cgaatgatca agaacgagag
    11161 cgtggcatta ctattttggc caaaaataca gcggtcgtct acaaagacac gaagattaat
    11221 attattgata ctcccggtca cgccgacttt gggggggaag ttgaacgaac cttaaacatg
    11281 gctgatggtg ccgtgcttgt cgttgatgca caagaaggtc ctatgccgca gactaaattc
    11341 gttcttaaaa aagcgctcga aattggttta cgtcctattg ttgttttaaa taaaattgat
    11401 aaacgcgatg ctcgtcccga tgtcgtaatt ggtaaggtgt atgatctgtt tttggatctc
    11461 gtgaccgatg aggcgcaact ggaatttccg gtatattacg caattggtcg tgatggtaaa
    11521 gcgtgggctg agcttcccgc gaatcctgat gaagatgcgg atctttcacc tctttttgaa
    11581 gctattttag aacacgtacc agctccagaa ggtgagagta atgctccttt ccaaatgctt
    11641 gtcacgacgc ttgatcgtga taattatcaa ggaaaatacg caattggccg tattcgccgt
    11701 ggtatcgcaa aaccagggtt acccgttgtg ctgatgggga gcgatgggaa acaagagcaa
    11761 ggacgaattg ataaagtgtt tgtcagccaa ggactcaaac gtgttgaagt accgagtgcc
    11821 gaagcaggcg acgttgttgc cctcactggt attaaagggg cgaatattgg tgaaacgatt
    11881 gctgacgcga ctcagcccga agccttgccg acaatcgcca tcgaagaacc cacactgcgc
    11941 atgtctcttt ccgccaatac ctcgcccttc gcgggtaaag aaggtcaata cgtaacgagc
    12001 cgtcatattc tcgaacgtat tcaaaaagaa ttagaaacaa atgttgccat gcgtttggaa
    12061 gttaaagacg gtggtgaata catcctgtca ggccgaggcg agctacattt gtcggtgttt
    12121 attgaaacgc ttcgtcgtga aggctatgaa cttcaggttg gtaagcctca ggttattaca
    12181 cgtgaaattg aaggcgtcat gaaagagcca attgaagagt taacgattga tgttactgat
    12241 gagtatgtag gagctgttac gagtgaagta ggacgccgta aagggatttt attaagccag
    12301 tccgaaaatg gtgatggctc tacccgtctt atttttgaaa tcattacgcg cggtttactc
    12361 ggactgcgta accagctttt gaccctttct cgcggaacgg ctgtgatgaa ctctctcttt
    12421 ctgcgttggg aaccagttgg agcagcgatt ccgaagctcc gcaacggtgt tttgattgca
    12481 tcagaagctg gcaaggctat gacgtacggc attgagaatg cccaagaacg tggcattgtt
    12541 tttgtaggtc ctcagacgcc cgtgtacgaa ggtatgatta tcggcctcaa tcagcgagaa
    12601 aatgatctcg agattaatat ctgtaaagaa aagaaactaa ccaacgttcg ctcaaatgca
    12661 gaaatttcag caaccattac acctccgctg aacctttcat tagagcaatc tctcgatttt
    12721 cttgaggatg atgagctcct tgaagtcacg cctaagaatc ttcggcttcg taaacgagta
    12781 ctaaacgcta cacagcgtgc tcgttcacga aaataaagaa ataaaaaaga gagcctcatg
    12841 aggctctctt ttttatttgg aagaacgcgg tttcactttg acttggaatg agagagctcc
    12901 tgtttctcct gctgcaagcg cggggatcgt ccatgttaac gtgttcgtac tggtatcttg
    12961 taatacggct ggggtagtgg ggagtgaggc ttcgatgact tcaaactcag aaggaatcgc
    13021 cgtcgtaaac ttggcctgac tagcagcttt ttcaccattg tttcgatagg taacttgata
    13081 cctaagagtc tctcctgtct ttgcttggac tgagtcggcc gctaccgtaa cttcgaggga
    13141 ggcttttcct tcaggttcta tgtcgttagg aatgacagct aatgaaagtg ctccagttgt
    13201 ttcgattgtt gcccggccca gtcctttttc cgatacggag aaaggtatcc gcgtattgtt
    13261 gattttgagg ctccagcgtt gagctggatc gaggtcggcg ataaagagat tgctcgtgtg
    13321 gcttgtggtg gtggttattt ggaaggcctc tcgaataagc cgtgtatctt ttattttact
    13381 aaacagactc gcgtcatagg ttaatacatc gccgttttta agagtcggaa ctggtttacc
    13441 aggttttgca ctaaagaagc tgagtgttgt ttgtgttccc tgttctaaag cgacgccttg
    13501 gacggtctta tctgccgatt caatgagtgt cggagtagct gggtttgcct cctctccggc
    13561 tgtcacaacg gagaggaatg tatcccattg ctggtcgata gtgggtttta tggcaatatg
    13621 gaacgcttgt tcgtagtcgt aaataaaacc attaaacttc tgggcgggtg ccttttcgtt
    13681 gacgattgtc ttctgggtgt tggtaggcag gaggtttgtg agctgaataa attggccgtt
    13741 ctttgttctc catgaagctt gattccctgt tactatgggt gcctcactgg tatggagaat
    13801 ccactctttc cgtccccctg aattttcaat cgtgcttctg gcgccgcctt catagcgatt
    13861 ttcagcccag ccatacagct gctttggatc gtctgctaat gttctgtcaa atataacgac
    13921 cgtatcgcga gagctgtctt ggctcgggag gtacaggaag cttctcgtcc attcatgaag
    13981 aaacgtagct ggcgggttcc agtatccgct tctatgaata gttcctccag cagatccaga
    14041 ggtatagaca taggttccgt tgggatcaac ttcttgagcg gtaattcctc gagcttcttg
    14101 ggtggcgctg tagccagaga atcgcataac gttacttgcc agagacgaga gagtatagcc
    14161 aattggatga gtgatcgccc attctccttt acggtacagt tggaaatctc ccgttatcat
    14221 ttgctgatga tcgacatagg cgttggcgtt taaaaatgaa ctcgtgaaga atgatgcggc
    14281 gttaccccat cctgtgcgga aatgagtgag cccttggttt gtcgtcgtat ggcttcgtgc
    14341 gagttttgtt tgccaatctg tggcaggagt ggctgcagga ttaaaaaata ggaaaaaagt
    14401 aaacggaggt gttttgacaa atgtatcgtt tggatacgtg tccatcagtg ttttgattgc
    14461 tccccataca agaggggcga ccgtcgtatc ggattcgttc accgcggcta atgcagccat
    14521 atgtggcagt tttccataga gtcggatgtc acgtggatgc tgctcgtctc cccactgata
    14581 attttgtttc aagtcagggg taaggatatg catcgattgg atggccgctt ctttaaaata
    14641 ggatgcgagt tcaggaaaat ggtcagtttc tgttgctgtt tttacaccca tatagccaat
    14701 cgccatgagc attaccgtgt taacgttata atctgatgat tcaatccact cgccgccctg
    14761 ggccatctct gcgtatcgct tgatagcatt gcgcatagtg cttgttttat tgtcgagact
    14821 attgcttgtc gcatcgaagc cgccaacatg aggatggtta atcaagaaag tactggttgc
    14881 tgggtcttcg tcctcaattg ttaaggccgt cataataacg ccaaaatagt gcccaacgac
    14941 ttcatctgaa tcatctaacc gtccactggc aaaatttgtg ccaggtattt ctcctatggt
    15001 cagttttgac caatatataa gacttgtacg gaatgtttgt cgctgctcag ccgatagtgc
    15061 tggtttgagc cagtcgtaga tgagggcata ggtcgccagt gattggcggg tcccgtttga
    15121 tccgtcagcg ggaagcgagg gtggattgct cgatacaaca gggctgatgc cttgccatgc
    15181 tttttgagcg taggcggtat ctcctgtcat ctgatacata agcgttggcc atgttccata
    15241 acaggcaaag cattgttgaa cagccgcatc agaaggaatg gcggcatcag cttttgattt
    15301 aataactgaa aaccatcgag gatcattttt gaggttattt aaaacagctt gacgttcagg
    15361 gctccaaaag agctttggcg cctgtccggt tggtgattgg gcatgcgtat actggctacg
    15421 tatgagccca gtcccaaaaa gaacagtgat aagtaataga atgctcaggc gggcggttat
    15481 gtgcttcata cgtgcgaacc tcgaaggtag agtaagctaa ctattgttcc gcatgcaccc
    15541 aatacgacta aggcgattgc aaatgatcga gcacgtgatc tttctgcgga tgttggtaca
    15601 gaggattgga tcgggtttgg ggttggtgtt atgtcgatga gtgcttcagg agacgactca
    15661 gtagaggggg tggtagttgt cagaggcgtt tcgctcggtg tcgctttgga gctcgctgtt
    15721 accgctacta ctatctgaga tgttgtagtc gctttgggag aggcttttgg tgttgaggtt
    15781 gctgcggggg aaatgctcgc agttggtgtt gctgtcggtg tgggtgttgg agttacagtc
    15841 gtggtgactg ttggagctga aatagtagta tgtgcagatt gtgtcgattg aagaatattc
    15901 gttaagtcgc tgtcggcttt tacttgggtg gtggcttctt ggatggtgat atcgcctgcg
    15961 ccaggctcat tggcaacaaa cgttatcttg ccaataagtc cttctgttcc tacatacgaa
    16021 gtattcatag ctacgcgtga aaagctaagt gtgttgccat tgaccgttgg aacatcgatt
    16081 tctgtatcaa aaattgaacc tgctccgtcg aacgattggt aggtcagggt tgatgaaaag
    16141 tagagcgtta cttgtgtgcc gataacggcc tgtccgccag gcagtaatga aagggatacg
    16201 gtgacttggt tgccttgggt taccgtggat gtaggtacac tgagtttcag tatggctgtt
    16261 ttgtcggcag cctgtgcgtc cccgagtggc catagcatga agctcgcaat gaataaggcg
    16321 ataaggcttt tatgcatcat ggaagtcctt ttgttgacga tatttcttaa tgatctcaag
    16381 cacatggctg aactctggtt tctcttctcc gaatacagcc ggcacaaact ggaggcttgt
    16441 gccccgatag gccttgataa taagcgtaac atcatcaaac gttagtgcgc catcattatt
    16501 aaagtcgcct cgtattaact tgtttgcctg tgcgtaatta atacaggtgt ttgcatcata
    16561 attttctacc gttatacttc gggcgaaata accatcggct ttgaaccgta aagttatagg
    16621 gagggttaaa gccggagttt cttctagggc aatcgtaatt tttccatctt tatcacttgt
    16681 aagatcttgg taggttgcaa gagtagttcc tttggtgtcg ctaataatag cggttccatg
    16741 caaaggtgtg actaataagc tctcggtttc cgcttgaagg caaatggcct gtaaaggagg
    16801 atcattctgg gtaacggtaa aggaaagaac gttacttatg gcatgctcac tatacaggtt
    16861 ctgagaaggg tcacaggtgt cagtcccttc gccttccgtg cagaatgcaa cgctcacttg
    16921 gcttgatgcc cttttctcgg tggaggcaga aggagtgaga gatttctttt ggaataaaaa
    16981 atataacccg gtgagtaata gagacaaaag tacaatagat agtgttttgt gatgccaaaa
    17041 agaaagacgg tggtttgtat gccgcatata cacaccttag tcaaccatga gagagaaagc
    17101 aattatattt ttaattcgtt aggagttacg aatgtggggc gttcttggct gttcggcgag
    17161 gagatcggct atggactcct ctcataatac gaaaaaccca aggccgtgga acaattcgag
    17221 ttgcaagtga accgattgta ttaagagttc cggggacgac gactcttttt ccattgagca
    17281 tgccgcggta tccaataaga gccactaatt cggcagacat taagcgatgc cgggtaaaaa
    17341 gattcgtttg gagcatgtgt gctcgagtcg caaatcctgt ttttgtagga ccagggcata
    17401 atgctgtcac agagatacca cttttcttta attcttcggc tagggcttca gagaaagata
    17461 gtatgtatgc ttttgtggca taatagacgg ccataaatgg tccgggttga aatgcggccg
    17521 ttgaagctac atttattata tgccccttat ggtgcccttt catctgcttg agtacaagtt
    17581 ttgtcacttc ggtagttgct gccacatgga cttcaatcat acctagttcg tagtcgaggg
    17641 aagtgtcaat aaacgaacct gataaaccaa atccggcgtt attaatcagt acatcaaccg
    17701 tgtggtggtc taaaattttt tctatcgtct tgcgacgttg taacgtatcg cttaaatcga
    17761 gtatctcaat cgatacggtt actccccaat ctctttctat ttcacgtgcg agagcttcaa
    17821 gcagtacttt attctgtgac agcagaatga ggttgtaccc ttgttctcca agaagtctgg
    17881 cgaaagctgc ccctatgcct tgggatgcgc ccgtaatgag agccgtttta atcatggcag
    17941 agtatttcgt tgcaaaaatg agagtgcata tctaaagtat agcagacttg ttcccatttt
    18001 tttaatggca gtccgtctcg gactaaagta taatgatact atgaaaaaag ttttaataac
    18061 tggtgggaat aaagggattg gcctagctct gacggagcta tttcttgaga atgggtacgc
    18121 tgtggcggtt attgctcggg attttaccca cttttcatat aaagaagata aacgcgttca
    18181 atgttttgcc cacgatttaa cggatattga ggcgattcct cagttagtac gtgaagtggg
    18241 ggaggtggat gtgcttatta acaatgctgg cattatgttt agtctgcctc atgatgagta
    18301 tccttccgat aagaaagagt acatactaaa acttaatctg aaggctccga tagctcttat
    18361 cgaggcggta agccgattaa tggtcacaaa aggagacggc cgcattgtta acgtcgcttc
    18421 gattgctgga gagattgggc atccggatat ctggtacgga gtaacgaaag cagctctcat
    18481 taatgccaca aaaagctttg cccggctttt aggtcctgac ggtattgtgg ttaattgtgt
    18541 tgctccaggg ccagttaccg ataccgagat gtttgaggtt gtccctcagg caagaaaaga
    18601 acagttacaa aaatcgacca ttacacagac gtttgcgacg ccagttgatg ttgctcaaac
    18661 ggtgttctgg ttggctgact cttccccaag ctatattaat ggtgtctgta ttgatattaa
    18721 caacggttct ttcccgcggt aaaaattata cgtaggaata gtgagactgg gataaaaaat
    18781 cgcggagagg cggtttgaac tttcctatga cttgagcatc ttcaaagaat tttgaaatag
    18841 gcattaacat ccatcgttgg ccctcatcgc ctagcacgat tttatcaatg gttgcctgat
    18901 tgataaaaca gaccataaaa gagaaatgaa gcgtcgtatc ttctggttcg gtataaagtt
    18961 tctcccatat aatagaattt tcattgacag cgatgcctaa ttcttcgttg acttctcggg
    19021 caagacactg tcggggtgtt tcatttcctt cttttcctcc tcccggaaga tcccacatgt
    19081 tggcataggg taactcaggt atgttgtcac gtaagagaac aacgattttc ttttcatgca
    19141 agagtgcaat tttggtgccc gtagagtcaa tgtatcttgc catgactatt tttgagagtt
    19201 catttgctcg aggatttctc gaactacttt tacgtcattc caagggataa gtttctctcc
    19261 tattaacatg acgtgttctc ctcctttgcc ggtaatgagg acggtatcac ctttttcggc
    19321 catcccaatg gctttggaga ttgcttctcg acggtctggt attttccacc accagtgatt
    19381 ttcgcctttt tttgcctctt ctgcgtttgg tcgtccgcgt gggatgcctc ctgcgacttc
    19441 attaatgatg ctggccgggt cttcggtggc aggatcttcg ttggtaacaa ttacaaaatc
    19501 agcatagcgt gcggcaaggg cacctagaat tggtcgtttg gttttgtctc ggtctcccat
    19561 cgatccgagt actgcaatga gacgtccttt caccataggg cggagcgttg agtagacttg
    19621 ttggagtgca tcgggggtgt gggcaaaatc gacaataatt gagtaaggtt gccccaaatc
    19681 aactggttcc atccttcctt ctacggggcg gagtttacga atgctttttg ttatttcctg
    19741 aaacgggatc ccgaggccga gggttatgcc aactgcgcac aaaacattgg ctacggtaaa
    19801 acgtcccggg aggggcatgc taacttctgt ctctccataa ggtgtcacca gtttaaacga
    19861 tgtcgtcgtt gagcccattt ttagccatga cgctgtaata tcggggtctt ttagtgcctc
    19921 ttttggtcgg agggggtcga gtatcccgta cgtgaacttt ttggacgctg gataactcaa
    19981 aaatgtcttg gcagccgggt cgtccgcatt cacgactgaa actttgggat tgttcgcaaa
    20041 aagtttttct tttgccttct ggtattcccc cattgtttta tggtagtcga gatgttcgtg
    20101 agttaggttt gtgaatccga cagcctcata gtaaattcca tacactcgat tttgatctaa
    20161 agcgtgagaa gttgtttcga caacggcgta ttcgcagcca gcgtcgacaa attttttgag
    20221 atgcatatga agttcacggg tgtcgagcgt tgttttcttt gtcttgttta attcgcgttt
    20281 gccattgata gagatggcga cggttgtgag cataccgact ttgtgacccg attcttccag
    20341 gagttgcgta atgtagtggc atgttgaagt ttttccattc gttccggtga ctccaataat
    20401 gcgaagtttt ttagcgggga aaccgtattg gctcgccata agagtcgact caatcttgcg
    20461 caacgattta atggttgttg gtgaaatacg attacgtaaa atagatttaa gaattttttt
    20521 catgattaat gacgccttat cgattcagcc gctttgaaga gagtatacca tagcgcttga
    20581 agggggacat cccagctgcc cataagatgt atggattccc ctccaaactg ttgtttaaac
    20641 cgagtaatgc cgtcgtacgc gtgcttttta tcggtgtctg gtttgatggc cagaagatca
    20701 tacgatgtgg cgtttttgct cattgcccat tgcatgacgg tccagtgcag cagatagggt
    20761 gccatcgcgg cgcgatgttc gcttgatgaa gcgccataca gataggacgc gacgccatca
    20821 taaagagtgg taattgatgc tgctaaagga attccctgga ggctggcgat aaaaactttg
    20881 ccacaattgt ggtggtagag tacttgggct aattcctgga aataggcttg agaacggata
    20941 gaaaacttgt cacgtttcgc ggtctcttca aacagggcat aaaagatagc cacgtcttct
    21001 tcaagtgttt ttagggttgt ctctcgcacc gttacgccag cccgttcagc tacccgtatg
    21061 ttgtagcgcc ccttttgttt catttgtttt aaagtggcgt gctctccacc tgaaagagtt
    21121 acgatttggc ggtcgtctgg ctggaccgac tcaaatgctt tttgaaatcc agtataagta
    21181 aaggccgccc gccattgctg cgcaagcgga tcagtcttag ggatatttag ttcgaggcga
    21241 aaaaataagc tgttacgtcg ttgggctact tcgtatatgg ctggcaggag agtgatgagg
    21301 agtgaaggat gatgaaaaac ttccggcgcg tataaaaacg attttccaag cggcaaaggg
    21361 cgttccaaga tgagaacatc ttcaagttta tgagcatgcc aaccctgggt ctcgcgaaag
    21421 gagccccaaa tatctgactg aagaaaactc ttcgggtaag gtgttttgtt ttgcattctc
    21481 aaatctctct ctgtatcata gggcaaggaa agagaaatta caaaatgcgt attctcgcga
    21541 tagaaaccag ttgtgacgaa accgctgcag ccgttatgga acggactgat acttcgccat
    21601 gggtgaagac aattacatcg gtggttgcct cacaggcaaa gcttcacgag aaatacggcg
    21661 gtgtgtatcc ggaggttgct tcacgtgagc atatccgtaa aattctcccc gttattgccg
    21721 aagcgctcga tctgcctctt cacgaacgcg gccgtttacc gaacttctcg gctgccggaa
    21781 ttgattacct ggcggtgacg gttgggccgg ggcttattgg ttccctgctc gtagggacgc
    21841 agactattgc ctcgcttgga tatgccacag gtcttccaat tctgccggtc aatcacttag
    21901 caggacatat ctacgccagt ttcattgcga cagagtctgt tctgacggcg cctgattttc
    21961 ctcttctctc cctcgttgtg tctggcggcc atacgatgct catttatatg aaggggcatc
    22021 atcaatatga attaattggt cagacacggg acgatgccgc aggtgaagcg tttgataaag
    22081 tggcaaaact cttggggctt ggatatccgg gcggtcctct tgttagccag ctggcacgaa
    22141 aagggaatcg tcatgcgtac aaattcccta tcggtttgga acatgaagat acatgcgatt
    22201 tctcatttag tggactcaaa acagcggtat tgcgcgaggt tcaaaaagca ggtgctctgt
    22261 cggagcaggg aaaggcggat attgcagctt cttttgagcg ggcagcgatt gaagccctac
    22321 tcttaaaaac ttcacgagca cttcaatcaa gacctgtccg tcattttatt ttagggggtg
    22381 gggtcgctgc caataactat cttcgtgagc ggttggcgac acttttggcg cacaaaacac
    22441 ccgcggtaac actccacgta cccccaagcc atttatgtac agataatgcg gaagtcattg
    22501 gtgcggccgc agcttttagt aatctcgttc cgattgctcc gtccgagcta aaaacatatg
    22561 ctcggtaccc tctgacgagt tagttactcg attctactgt gcttgcttaa gtgtgttaag
    22621 cattgcttga tagacgatga gcaggtcgtc gtcggcaggt gtgctgactt cattaaaaaa
    22681 tgtcacgcca agaattgctt cgggatgatt aatataagta tccgtaataa ggtgtgtttg
    22741 tacattttca taaccggcgg gaatgtcgga atacgtcacg gcttctaggc cgtcaacacg
    22801 aaggaagaga cgttgtttat actctttcgg agccaggccg gtactatttt tggggtatat
    22861 aaagacgacg ataggtcctt gaccgtcgga gtcagggtta aagtctggtg cctcaggtgg
    22921 aaaaaaatga atttgagtcg aatcacggaa accaatcttc caatcagcgg gatattgaat
    22981 cgaaaagtgg aatgcttcgt tagtgtatgt tttccatggc tccggagttg ccgtcgccgt
    23041 tggctgtggc tcggcggctt tgccatggct tgctgtgtag tcatacatca aaaggccacc
    23101 gctgactaaa aaggcagcta aaataatgct caaacttaag agtgccgagc gagttcgggg
    23161 taataattga aatgatttct tgagcttttt gatgaggtta atcatgtgct cgtattatat
    23221 acgaaaatgg ctaaaaagcc aagggttagc tgttctcgct tggaggttgg tgtggtagaa
    23281 taacactatt atgcaagact ttccaaagcg cttcgactca gcgactgctg aacccctcat
    23341 ttacgataaa tgggttgcct ctggtggctt ttccccttct atggagagcc aagcagaagc
    23401 ccgttctatt ttaatgcctc caccaaatgc gaatggagcc cttcatttag gccacgccta
    23461 tgaaatcgcg attcaagata cgcttattcg ctattggcgt atgcgtggtt ataaaacgct
    23521 ctggcagccg ggagccgatc acgcaggttt cgaaacacag tatgtttttg atcggaagct
    23581 ggaaaaagaa ggccgcaacc ggttttctat cccgcgcgca gagttgtacc aagaaatcta
    23641 tgattttagt atcgctaacc aggccgtgat ccgccaacag tttgaacgga tgggagcaag
    23701 cgctgactgg tcccgtttcc ggttcatgct cgaacctgat ttggtgaaaa ctgtttataa
    23761 aacctttaaa cagctctacg acgatggcct tatctaccgt gccaaacgac ctgttcactg
    23821 gtgcataaaa gatcaaacga cgctctccga tttagaggtt actgaccaag aacaaacaga
    23881 taagctctat tatcttacct atgggccggt taccgtcgcg accgtgcgcc cagaaactca
    23941 gtttggagat gtggcgattg ctgttcaccc cgaggatgag cgctacaaag atttagttgg
    24001 cacgactgta gacgtgaaga tggctcacca aacagtatca ttgcccgtta ttgccgatga
    24061 ttacgtcaac cgcgaatttg gtacaggcgc cttaaaaata acgccggctc atgacccaaa
    24121 tgatttcgag attgctcagc gtcataattt ggatctccca atcgtgattg atcaatatgg
    24181 caaattgact gatcagtgcg gtcaattttc ggggatgaaa attgcagatg ctcgtgcggc
    24241 agttgtggct gagcttgaat cactcggagt ggtagagaaa attgaagcct atcctcatac
    24301 ggtaaaggtt tgttataaat gtaaccgtct gattgagcct cgaatcattg atcagtggtg
    24361 gttgtctctt acgaaaccaa gccagtctaa acaaaaatcc cttcgtgatt tggctgtagg
    24421 ggcggtggcg tccggtgaaa cccgttttat caccgaacgg tatgaaaacc agttccatag
    24481 ctggatggaa agcgtgcgag attggccact ttcgcgtcaa attgtatggg gtattcaact
    24541 gcctgtttgg tacgccaaag acggttctgt cgtggtgacc gaaggcgaag agccggccaa
    24601 cgcttctgaa cttactcgtg acagcgatgt ttttgatacg tggttttctt cgtgtcagtg
    24661 gcctttctca acgctgggaa atcacaatga cgatctggaa acattttatc ccacaacggc
    24721 gatgacccca ggatacgaac ttatcttttt ctgggttgct cggatgatga tgcttggtat
    24781 ttatacacaa ggcaagtcgc cctacaaggt ggttatgctc cacggcattg ttcgtgatgg
    24841 caaaggacaa aagatgtcca aatcgaaagg gaatactgtc gacccattgg cgacagccga
    24901 tcaatacggc gcggacgcta ttcgcctcgc tttattatat ggtacggctc cgggaacgga
    24961 ccctacagca agtgaagaga agattcgggg aatgcgtaac tttggaacca aggtttggaa
    25021 catcgcccgc tatattacga tgagtaagtc agcagatgct aaatcagagt ttatacctgt
    25081 tacagacgcc gaccataccg tgattaagca attacattcg acgattgcca ctgtcactca
    25141 ggcgctggat gactaccaac ttcacattgc gcttgagtcc ttatatcaat ttatctggcg
    25201 tgatgtcgcc gatgtttatt tagaaaaaac caaagaccaa ctccaaaatg agaccaccaa
    25261 gctcacaaca gaggggaatt tagtgtacgt attagacact atccttcgtc ttcttcatcc
    25321 ttttgcgccg ttcgttacgg aagcattgtg gtcagaggtt atgcccggtc aaccactctt
    25381 gcttgttagt cagtggcctg aggttacgaa tggttgaatt ccaagtgccc ccaacctacc
    25441 gggtcgcaac gagactggca tcgaatgtat cggtagcccc tcgattgtcg tctcgattcg
    25501 cgataattct cggtagtgtt tttttcggtg ggcttatagt tattaagctc attgccggtg
    25561 ctatatatgc agaagaaact cgcttattgc cgagcgctga gacggaaata ttaccaacat
    25621 accagctcac tgttaaaaag atgaccaatg ttgaaaaggc aatgaaatcg gccgataggc
    25681 tcgtttctaa aggctactat gatttttctg aattactgta cgcgcaggct tctactcttg
    25741 atcctaactt acgagatgct tcctatggat gggcttactc gattataaaa aagccacact
    25801 tagcctccaa ggatctggtg gatctccata ctgctatgag tcgggccgaa gccgtagatc
    25861 ctctctatcc tcctcttctc cagttaaaac ttattgtcgc gactttagaa aaagaccaat
    25921 caacaaccac tgcaacgcaa gctcgtctcg attctctcaa gttttaataa atattttaac
    25981 ttcttatcgt atagtattat agctgtcgct atagtacttc cgatggaata tgctatggtg
    26041 aagatgtatt tcagcaactc ggtcacggta gaaaggggaa atcagtggta cgtcgactgt
    26101 tcatgctggc cctggtggcc ggtatcgttt ctctgggtgc ggtagcgccc gtgcgttcac
    26161 aggagaaggc cgctccggtg aaacgcgacc cgcggctaat cgccaaatgg gaactctcta
    26221 acggcgcctt cttccgtttc tgggatgatg gtgaggccga aaagggatat ggacccatac
    26281 tgaagcacgg tagctggcgg accgagaaga cttcaacggg aaaggatcaa ctcatctgcg
    26341 tctggcgcga cgatgcagtc tttcccaatt cagaaggggt ttacaccctt tcatctgacg
    26401 gaactatcct cactctcgac attgtcgagg gaagggatct caagaaagaa acaccccagc
    26461 gtgtgaagtt ggagaaagtg gtgaggaagt aatccctcag gaaaagctgg tgatctgcat
    26521 gaaagcctct gggccgcacg agcggtccgg gggcttttct cgttacgggt aactttgcaa
    26581 tcaagtcata aaagttgcta tagtgaatat atgttaaaag gatggggctc ttattttagt
    26641 gtctgtgggc tcgtaatggc tttttttata tcgttatttt ctcctgttca tgttgtagct
    26701 gctggagaga gcttgttagg tttaacagcc accttagtac cttccgggag tacttctcag
    26761 ttggtcgtta gttttagaat tcaaaattat caatggcaat cgaacaaaag attccgcgtt
    26821 tctgtctcca ccgacaaccg tacagataaa gaagttacat ttaaagctgg aaaaggtgga
    26881 ccaaactact cacaaacaaa ttacacggtc tacatcgctg gttatcgtcc ggtagcaggt
    26941 ggagctgggg tgataaaagt tacattgtcc gatcaaggtg tcatcagcca tagtcctata
    27001 acctctagta ctacacgcgt aaccacgcct accccaccga aatttcagga agtaagtgtc
    27061 tatagtgatt attcatcagc tgctggtaaa aatgtgattt gggttaagag tacaatttta
    27121 cccgacagtt accgcagtgg tctgacgcta cgggtcgtaa ataaagaaac gaacgcccag
    27181 tatattgagg caaacaacat tacgcaggta tatgcaaatg caaccaatgt tgatgctcct
    27241 tccactgctc agcaagttag atttacgctt tatgagggcg ccacggtact tgataccaaa
    27301 gatgccaccc tgggaagtat cgatagccaa gtggagatta ccgtaggttc cactccccag
    27361 ggggggcttg tgaaagtttt tctggacggg gtagacaaag gtaccacacc agttacgtta
    27421 cgaccagaca aagataccga ttcagagcca gctcaacata aaattaaaat taccaatgga
    27481 acagtcgaaa aagagtttga cgttaccttt gaggctaacc atacgctcta ttataacttt
    27541 acgaccgaag gtggaggggt tgacgccgat cctactgaag ccgataagcc gaaaacgaca
    27601 acagcaggag ctgcgggaca atcaaagccc tgtaacgaat atttgggttt ttcttttggc
    27661 agtggattgc agttcaatat tccagcctat attttgtgtc agtttaattc gcttattcaa
    27721 ttgattgcaa aaacaaccca aggagttatt gatggtctcc cgctctagat ggcttcttag
    27781 tggtttgtta ctaattgtgt tgggggtact tttcttccct gcctctaccc aggctgctct
    27841 tgctcctggt acagatcatc gggtatggac gtgggttgag gggacgaacg gtgtcattaa
    27901 tgtatataag gtcgttcttg ggctagtgga ttttgtagtt gtattgggac tattggctgc
    27961 cgcgtttgcg aatatcttta gctttatccc tataaagctc gatagttaca aggtgaagca
    28021 agtgttgccc gggctcatta ttggtattgt aatggcgaac ctgagctatt ttattatgca
    28081 cctctttatc gaagtgagta cgattatagt tcagatgatt gggattattg tatcccagca
    28141 ggtaggggac acgattggaa cgcagtcagg gaccgggtac attattacta aattatggca
    28201 agaaattctc ggatctctct ttaagcttcc gaccgcatgg gctggtttaa gtggtgtggt
    28261 tactgctatt gcaggaggag gtgggcttat tgctgctggg gcaacactta ctgaaatgct
    28321 tgcggcgata gggggatccc tcattcttaa ccctgcaggt ggtgctatag gtattatgtt
    28381 cggtcttgtt ctgctggcgc cactgattat tgctttgatt ctcgcttttt tactttacat
    28441 tcgaggctac attctgatta tctgttttgt tttatcccct cttgcctttt tttcgctcgg
    28501 ttttccaccg ttaaaaacat tttggaataa gtggtggacg aacttttgga agtggttaat
    28561 tatggcacct ttggtgtttc ttgttttggc agggggtatt atttttatcc attttgccaa
    28621 taatgcagct ggtggaagcc gtaacctgac ggattatatt ttctttaatg gagtagggat
    28681 tggattctta gcagttgcca tgaaactgcc attttcgttt ggtaccttct ttggtgcgaa
    28741 tgtaatgaaa gagtacaaca actggagtaa gcgtgctgct aaaacgggct gggatggagg
    28801 ggtgcgcgga acggcgtata ttggaggggc gcagaaggcg ctacgaggac atgcgggaag
    28861 ttggacaaat cgagcagatc ttaaagataa attcgctgct gctaatgccg ccggaaaagc
    28921 ggctatgact acggataaaa ttacgaaagg ttggcttcca aataatccta ttcgactagc
    28981 cgaaggtgtg cgagcgggta tgaaggctcg tcttgagacg gcagataaag ctgatgccaa
    29041 aatgattaaa aagacggtta cttatagggg ggcagcaagt cgggaggcga aatatcgaga
    29101 ttatacagaa tcaaacgggc atattaagga agaaaccgat cctcgaactc ttgttgatga
    29161 ccatattata aaaaatggcg gtgataagtt tacgccagaa gaactcaata aacttcttac
    29221 tgagtcgcca ggggagctcg tagagttacc gatcgtacga cgggcggagc aagaaggggt
    29281 tgaccttgaa aagttggcca agcttatgat tgcttggaaa cagtacaagt ctataagtgg
    29341 cagaaagaat attaaatcag gagttcttga tcctccctat ccaggaagag ggtcaattgg
    29401 tgatccagac tatgttcctc ctcgagatgc tggtcgaggc tataatgctc ttgttcgaga
    29461 tgctggcaca ggtagaatta ccggttttaa tcaacaaaga ttcattcaag gtaatagtga
    29521 cgaagatgtt atgggggcgc acgttgggcc caatcatagc gatgacggta actcttcttc
    29581 ggaagatgga aataataatc ctggcggtaa caaacctgct gcccccaaag gtccacaggg
    29641 gggtggatca ggtgggaacc agagtcctca gcgtggcgta gaacaaggtg attcgtctca
    29701 tcctctgcta tctcagtcgt tagataccgc tactccttca gttaacatcc ct
//
LOCUS       DASWYB010000072         6629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_92700, whole genome shotgun sequence.
ACCESSION   DASWYB010000072 DASWYB010000000
VERSION     DASWYB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92700"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..2567
                     /gene="recQ"
                     /locus_tag="VGE59_04205"
     CDS             369..2567
                     /gene="recQ"
                     /locus_tag="VGE59_04205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEY1074867.1"
                     /translation="MPTDSLSRANTLHTLLKTHFGYDHFRPLQEEIITHITAQKDAFV
                     LMPTGGGKSLCYQLPALYFEGITLVVSPLIALMKDQVDALTANGIPAAFLNSSLTVGE
                     MKRVQEKALQGELKLLYIAPERLAVPDFQSFLQSLPVSLVAIDEAHCISEWGHDFRPD
                     YRNLRRLREQFTAVPVVALTATATHRVREDIIEQLGFQNSPTFISSFNRDNLSYHVRP
                     KRNSFMGLVDILKKHPQESAIVYCFSRSDTESMAANLREAGFTALPYHAGLSSETRKA
                     NQDKFIRDEVQIMVATIAFGMGIDKPDIRLIVHMDLPKTLEGYYQETGRAGRDGLPSE
                     CILFYSYGDKMKQDFFINQLEDPLERTNAQQKLTHVIDFCQLETCRRAYLLHYFGEMW
                     EKQSCDACDACLTPREQFDATEITKKILSAVVRTGERFGLKYVTDVLIGAKDKKIRER
                     GHDMLSVFGIARDMPESDVMQIMASLVTRQFLMKNEGEYATFRLSQRAKQFLLENESI
                     MLAKPLVREISLSRRKKTKGELEYNQDLFEVLRVLRKELADARGVPPFVIFGDATLQE
                     MATYYPRDNQSLREITGVGDTKLKQFGPTFLPVISDFVTQHDIPDARPISTLRLVRRG
                     AEEKEGLTSTYLETKRLLDERFSVEMIAKARNLTEQTVLSHVEKLLLEGTDVITDHLK
                     FEVEDYKRTAIEKAFLQSGTESLSQVKAALGDAYTYTDIRITRILLRHHL"
     gene            2648..3127
                     /locus_tag="VGE59_04210"
     CDS             2648..3127
                     /locus_tag="VGE59_04210"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEY1074868.1"
                     /translation="MDMLQRTISIIIVLLIIVFGAIWLRGNGQNGTVTVSPSPSAAVT
                     ITATATASANPLIEVTLPHADSLVTSPLTVTGQARGTWFFEGQFPIRLVDATGKEIAR
                     GTAQAKGEWMTEEFVPFEGTLTFSKPSTTTGSLIAQKDNPSGLPQNDQSISIPVRFQ"
     gene            3318..3641
                     /locus_tag="VGE59_04215"
     CDS             3318..3641
                     /locus_tag="VGE59_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY1074869.1"
                     /translation="MVKKLYVGGIAYATTDDGLRQAFEQAGTVETASIVIDRESGRSK
                     GFGFVEMATEEEAQAAINMWNGKELDGRTLVVNEARPKEDRPSNGGGYRSGGSNFGGG
                     YRGNR"
     gene            3918..4511
                     /locus_tag="VGE59_04220"
     CDS             3918..4511
                     /locus_tag="VGE59_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY1074870.1"
                     /translation="MWGNRKGEGGIILQSPASPSYAPLPPEEMGQGSNVPYIFGILVM
                     VLVLATWGLLSLLESTRTSQAVEAKARSKQLDTQLSDPALQSVLGKYVAIQTVSKHMT
                     TLLNDRYIFTPTWQAIKSSVPPEVQFTTVTMAQDYRFRITGTTKSVAALAGFSKTLAS
                     QSTISNVSPASLEKAGDSYRFSLTFSVTPGAAGVAHE"
     gene            4504..5022
                     /gene="pilO"
                     /locus_tag="VGE59_04225"
     CDS             4504..5022
                     /gene="pilO"
                     /locus_tag="VGE59_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY1074871.1"
                     /translation="MNRNTLGFLLFILAIMIGVGAVYPKVRSVHMASVQKDAELKVVA
                     LKKQRLERLKLLNQTFDTESVKVGKVLSALPNEPAYPEALVSLETIARESGVLIQSLL
                     PQTDTDRQEISITLAGDGGLANVEKFLDSLASNDRPISIAAVSITKANAQQVSFTMTV
                     RLPFVDVAGATQ"
     gene            5019..5282
                     /locus_tag="VGE59_04230"
     CDS             5019..5282
                     /locus_tag="VGE59_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074872.1"
                     /translation="MKHEWLSSLIILAFVAAILGYGILAAQPRTSEVNAAVEEMLSVS
                     ERTAIDTSILSQPTIDKIQDFKVFGTRPTPAAPLNRTNPFEGL"
     gene            5282..>6629
                     /locus_tag="VGE59_04235"
     CDS             5282..>6629
                     /locus_tag="VGE59_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY1074873.1"
                     /translation="MSDLVRKTAIQQEEAPTVDLAHESIGLDVLHLLPFTTAQQYRAI
                     VFRDVTSDGKRTVSVAAEDPTSDTIRGLARYLEDKNHVSVEVYRVEAADIDSQLARYQ
                     QAEASQGVGEEGEPVVSTPQKDIETKQDLDAVIQQAVIPNIVTAIINFALARKASDIH
                     LEPLEDVVRIRFRLDGNLVDITNIPRFFRNAVTARIKILSQMKLDESRIPQDGRFEFD
                     YHAHTIDIRVSTLPTVQGEKIVMRLLDKDAGVMTLEELGITGSSFDALVRAVSRPYGV
                     VLSTGPTGSGKTTTLYAILQRIAHPDTNVVTLEDPVEYEIAGVNQSQVKPSIGYTFAE
                     GLRAVLRQDPNVIMVGEIRDLETASMVTHAALTGHLVLSTLHTNDAAGALPRLMNIGV
                     EPYLLTSAVNAIIGQRLVRKLCVACRQQVDLPKALLEEVRAELNTLKGFPISNQTDY"
BASE COUNT         1787 a         1420 c         1599 g         1823 t
ORIGIN      
        1 gtcttaccga taaattcggt gttgactgga tggtaaacat tagcaacgaa aaggcataag
       61 aataaaagat tctaaagaag cagcatagtt gctgcttctt tagaataacg cgttgaaagg
      121 agccgttata aacactgccg actctcagaa cactttttct tatctttcag ctccggcaag
      181 gagagcgctc gcccgtgctg gttataccca acttgaggaa ttaaccaagg tgagagaatc
      241 ggaattggca ttacttcatg gaatagggcc aaacgcttta ataaaactcc gtgctgcatt
      301 aggccaagaa gggctcgcct tccggcccga ggagtcataa gggcggtact ttttgatata
      361 ctcagggcat gccaactgat agtttaagcc gagcgaatac tcttcatacc cttttaaaaa
      421 cccatttcgg atacgatcac tttcggcctc tccaggaaga aatcattacc catattacgg
      481 ctcaaaaaga tgcgtttgtc ctcatgccaa cgggtggggg aaagtcgtta tgttatcagc
      541 tcccagcgct ctattttgag ggaataacgc ttgttgtctc tcctctgatt gcattgatga
      601 aagatcaagt ggatgcgctg acggcgaacg gcataccggc agcgtttctc aacagtagtt
      661 taacagtagg agaaatgaag cgagtacagg agaaagcgct tcaaggtgag cttaaactac
      721 tgtatattgc tcccgaacgt ttagccgtgc ctgactttca aagtttttta cagagcctgc
      781 ccgtatctct ggttgcgatt gacgaggcgc attgtatttc tgagtggggc cacgattttc
      841 ggcccgacta tcgtaattta cgtcgtttac gtgagcaatt tacggccgtt cctgttgttg
      901 ctctcactgc tactgcaact catcgtgtgc gtgaagatat aattgagcaa ctcggatttc
      961 agaattcacc gacattcatt tcgagcttta atcgcgataa tctgagctat cacgtccggc
     1021 caaaacgcaa tagttttatg ggtcttgttg atattttaaa aaaacatcct caagaatcag
     1081 cgattgtgta ctgcttttca cgtagtgata ccgaatcaat ggcggccaac ctgagagaag
     1141 ctggatttac ggcactaccg tatcatgctg gattgagcag cgaaacgcgg aaagctaatc
     1201 aggataagtt tatccgcgac gaagttcaaa taatggtagc aactattgct tttggaatgg
     1261 gcattgataa gcctgacatt cgtttgatcg ttcatatgga cctgccgaaa actctggaag
     1321 ggtactacca agaaaccggc cgcgcagggc gtgatggcct gccgagtgag tgtattctct
     1381 tctattccta tggcgataaa atgaagcagg acttttttat taatcaactt gaagatcctc
     1441 tcgagcgaac aaacgcgcag cagaaactga cccatgtgat tgatttttgc cagctcgaaa
     1501 cgtgtcggcg ggcttattta ctgcattact ttggagagat gtgggagaaa caatcatgtg
     1561 atgcgtgtga tgcttgcctt actcctcgtg aacaatttga tgccacagaa attaccaaaa
     1621 aaatcctttc ggcggtagta cgaactgggg aacgctttgg cttaaagtat gttaccgatg
     1681 ttctgattgg agcgaaagat aaaaaaataa gagaaagagg acatgatatg ttaagtgtct
     1741 ttggtattgc tagagatatg ccagagtcag atgtgatgca gattatggcg tcgcttgtga
     1801 ctagacagtt tctcatgaaa aatgagggag aatacgctac ctttcgctta agtcagcggg
     1861 cgaagcaatt cttactggaa aatgagtcga ttatgcttgc taaaccgtta gtgcgagaaa
     1921 tctcattgag ccgtcgtaaa aagacaaagg gcgagcttga gtataatcaa gatttgtttg
     1981 aggttctgcg tgttttgcgt aaagagctcg ccgatgcccg aggtgtgccg cctttcgtta
     2041 ttttcggcga tgcgacactc caagagatgg cgacctacta tccccgagat aatcaaagtt
     2101 tacgagagat tacgggtgtg ggagatacga agcttaaaca gtttggtcct acttttttac
     2161 cagttatttc tgattttgtg acgcagcatg atattccaga cgcacggcct atttctacgt
     2221 taagactggt gaggagggga gccgaggaaa aggaaggtct cacttctacc tatctagaaa
     2281 cgaagcggtt gcttgacgag cggtttagtg ttgagatgat tgcaaaagca cgtaacctta
     2341 ctgaacaaac cgtgctatcc catgttgaaa aacttcttct cgaaggtaca gatgtcataa
     2401 ctgaccactt aaaatttgaa gttgaggatt ataagcggac ggcaatcgaa aaagcctttc
     2461 tccaaagcgg gactgagtca ttatctcagg taaaagcagc cttaggggac gcctatacct
     2521 ataccgatat tcggattact cgtatattgc tccgtcatca tctatagtat atgaataagg
     2581 tggacgcact tacgtacagt tgtattggaa tatttgccct ggcttaattt aaaaaaagga
     2641 acatagtatg gacatgcttc aacgtacgat ttcgattatt attgttttac tcatcatcgt
     2701 gtttggtgcg atatggcttc gagggaatgg tcagaacggt acggttacgg tttctccttc
     2761 tccctctgct gcagtaacga ttacagctac agctacagcg tcagctaacc cgctcatcga
     2821 agttactctc ccgcacgcag actctcttgt gacaagtccg ttgacagtta cggggcaggc
     2881 ccgtggtaca tggtttttcg aaggtcaatt tcccattcgt ttagttgatg caacgggcaa
     2941 agaaattgct cggggcacag cgcaggccaa aggtgaatgg atgacagagg aatttgtgcc
     3001 gtttgaaggt actctcacgt tctcgaaacc ttcgacaacg accggaagtc tcattgccca
     3061 aaaagataac ccctctggat tgccacaaaa cgaccagtcg atttcgatcc cagtgcggtt
     3121 tcaataatta aggggtaggc atcgttgaga aggatagttg acatatccat agacattccc
     3181 tagggggtac ggtatactat agctactgta agagattctc tattcgtttg catcctactc
     3241 gtgtgtgttt gtcgcgtgcg gtgagagata gagaagggtc gacttacgca cggattaagt
     3301 aagattagga tgtgaaaatg gtaaagaaac tctatgttgg tggcattgca tatgctacaa
     3361 ctgatgatgg tttgcgccag gcgttcgaac aggctggaac agttgaaact gcttcaatcg
     3421 tgatcgatcg cgaatccggc cgttcaaaag gctttggttt tgtcgaaatg gcaaccgaag
     3481 aagaagctca ggctgcgatt aacatgtgga atggcaaaga actcgatggt cgtactctcg
     3541 ttgttaacga ggctcgtccc aaagaggatc gccccagcaa tggtggcgga taccgaagtg
     3601 gcggaagcaa ctttggtggt ggttaccgcg gtaatcgcta atagccaatc atcatccgtg
     3661 agctaaggct ctcggtatgc tggctcgagc ggtatctcgt ctttagcgag gtatctatat
     3721 atcgcgccac tggctaacgc ctacggcctg atgcgaataa gcacaaaaaa cagccccgat
     3781 gctcgcttgc gagctacggg ctgttttttg gtatgtgtaa cgccatattt tgttcgctta
     3841 tgtgctacta aaacaggttg acaagctttc tttggctatg aaatgatgat cttacagcaa
     3901 acggtatttt gcctcgtatg tgggggaacc gtaaaggaga aggagggatt attttgcagt
     3961 cacctgcatc accatcatac gcccctcttc ctcctgaaga gatgggccaa gggagtaacg
     4021 tgccctatat ttttggtatt ttagtaatgg tattagtcct tgcaacgtgg ggattattgt
     4081 ctctgcttga atcaacacgc acaagccaag cggttgaagc caaggctcgg agcaagcaac
     4141 tagacacaca attaagtgac cctgcgcttc agtctgtttt gggcaaatat gtggccatcc
     4201 aaacagtttc aaaacatatg acaactttat tgaatgaccg ttatattttt acgcctacct
     4261 ggcaagctat taaatcgagc gtcccgccgg aagttcagtt tactacggta acgatggctc
     4321 aagattatcg ttttcgtatt actggaacga ccaaatccgt cgctgcattg gcgggattct
     4381 caaaaacatt agcttctcaa tctactattt cgaatgtttc acccgcatct ttagaaaaag
     4441 caggagatag ctatcgtttt agtttaactt tctctgtcac gccgggtgcg gcaggagtgg
     4501 ctcatgaata gaaatacgtt agggtttctt ctttttatcc tcgctatcat gattggggtg
     4561 ggagctgttt atcctaaagt tcgctctgtt catatggcga gtgttcaaaa agatgctgaa
     4621 cttaaagtag tagctctgaa aaaacagcgt cttgaacgct taaaattgct caaccaaaca
     4681 ttcgatacag agtcagtcaa agttggaaaa gttttatcgg cgttacctaa tgagcctgcc
     4741 tatccggaag cgttagtctc acttgagaca attgcccgcg aaagtggcgt tttgatccaa
     4801 tcgttgcttc cgcaaacaga tacggatcgg caagaaatta gtattaccct tgcaggcgat
     4861 ggtggcttag ccaacgttga aaagtttctt gattctcttg cttctaatga tcggccgatc
     4921 tctatcgctg cagtttctat tacgaaggca aatgctcagc aagtttcttt tacaatgacc
     4981 gtacggcttc cttttgttga tgttgcagga gcgacccaat gaaacatgaa tggctgagtt
     5041 cacttattat cctggctttt gtcgccgcta tcttaggtta tggcatttta gctgctcaac
     5101 cacggacgag tgaggtcaat gcagcagtag aagaaatgct ttctgtctca gagcgtaccg
     5161 caattgatac atcgattttg tcccaaccta ctattgataa aattcaagat tttaaagttt
     5221 ttgggactcg tcctacccct gcggccccac tcaaccgtac gaatccattt gaagggctct
     5281 aatgtcagat ctcgtaagaa aaacagcaat acaacaggaa gaagctccta cagtagattt
     5341 agctcatgaa tctatcgggt tagacgtctt gcatcttctt ccttttacga cggctcagca
     5401 gtatcgcgcg attgtctttc gtgatgtaac atcagacgga aaacgaacgg ttagtgttgc
     5461 agctgaagat cctacgagtg atacgatccg aggtttggct cgctatctag aagataagaa
     5521 ccatgtcagc gtggaagtgt atcgtgttga agcggcagac atcgattcac aattggctcg
     5581 ctatcagcaa gccgaagcaa gccaaggtgt tggagaagaa ggcgaacctg ttgtgtcaac
     5641 tcctcaaaaa gatattgaaa caaaacaaga tctcgacgcg gttattcaac aagccgtaat
     5701 tcctaatatt gtgacagcga ttatcaattt tgcccttgct cggaaggctt cggatattca
     5761 tttagagccg ctcgaagacg ttgtccgtat tcgttttcgt ttggacggta acttagttga
     5821 tattacgaac atcccacgat tttttagaaa tgccgtgact gctcggataa aaattttatc
     5881 ccagatgaaa cttgacgaat ctcgtattcc ccaagatgga cgattcgaat ttgattatca
     5941 tgcgcacact atcgatattc gtgtttctac gttgcctacc gtgcagggtg aaaaaattgt
     6001 catgcggctg ctcgacaaag atgctggggt tatgacccta gaagaactgg gtattacagg
     6061 cagctcgttt gacgcccttg tacgagcagt cagccggccg tatggggttg tcctatctac
     6121 gggaccaacc ggaagtggaa aaacaacaac tctttacgca atccttcaac gaattgctca
     6181 cccagatacg aacgtggtaa cgctggaaga tcccgtagag tatgagattg caggagtgaa
     6241 ccaaagccaa gtcaagccca gtatcggtta cacctttgct gagggattac gggccgtatt
     6301 gcgtcaggat ccaaatgtca taatggtagg tgagattcga gatttagaga ccgcttcgat
     6361 ggtgacacat gcagctttga ctggacactt agtgctctcg acgcttcata ctaatgacgc
     6421 tgctggagcg ctgccccgtc tgatgaatat tggcgttgag ccatatctcc tgacatcggc
     6481 cgtcaatgca attattggcc aacgtttagt tcgtaagttg tgtgtggctt gtcggcagca
     6541 agtcgatctc cctaaggcgc tgttagaaga agtgcgggct gaacttaaca ccctcaaggg
     6601 atttcccatt agtaaccaga cggattatg
//
LOCUS       DASWYB010000073         7620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_1006513, whole genome shotgun sequence.
ACCESSION   DASWYB010000073 DASWYB010000000
VERSION     DASWYB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006513"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..624
                     /locus_tag="VGE59_04240"
     CDS             49..624
                     /locus_tag="VGE59_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY1074874.1"
                     /translation="MKKVIAGMTMSLDGYINDPNGSIEKLYSDFSELNNAPSFQGMIH
                     NTGAVIMGRNLYEMQDPFMWANDDYEFQVPIFVLTHTPPTKYPKGNDNLGFTFVTDGI
                     ESALSQAKNAAGDKDVQIIGGANTIQQYLNTGLCDELLIDIMPVLLGKGLSLFENINT
                     NKITLERIKVEETTSLRTSLTFKVTNRNHQN"
     gene            complement(674..1315)
                     /locus_tag="VGE59_04245"
     CDS             complement(674..1315)
                     /locus_tag="VGE59_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074875.1"
                     /translation="MKLTITYDLDKEFDNLQRGLKTVFNPEHQSKLVREMMAAKVNLS
                     SKEEVDKFIAQKIQDENINSQQAAHDIQKKWDKISQSAISRMDKLFATSAPFENITVY
                     LSLNGRCAYNFPERYFFVCFTRPNPIGVMIHELLHFYTHLLIQPYFIEQGEEDKHGEF
                     KEALTVLLNTEFNDVIEEQDKGYEKHAESRAWIESNWTKGMTVQELAKRYLER"
     gene            complement(1323..2243)
                     /locus_tag="VGE59_04250"
     CDS             complement(1323..2243)
                     /locus_tag="VGE59_04250"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY1074876.1"
                     /translation="MHDELIFKIINRHFHTQPSVVTRKTTGLCNEVYEAKLPDQSVIV
                     RIGVTNNNLLGSSKYIPMFQQQGINVPELLAEDYTGTFIPYPYHVLSMLEGQDLPDVI
                     AELNHDQLTNLAHEIAHIVKTLEKLPTNGKFGWVGADELHLVDSLTIEIENELQRANE
                     RMRQTGIFDEDLWQKVNDIFQSNKTYFDNAKSLFYYDDLNSKNVMIHAGKFNGLVDLD
                     GVKYGDPIQSIGAIRANWPGTEYGDFYTEQVMKALDLTNEQKRLVDVYAVIHRYSWMS
                     ENGIQFNANTQAAVNQDKLARDKEIISFLV"
     gene            complement(2258..2806)
                     /gene="cyaB"
                     /locus_tag="VGE59_04255"
     CDS             complement(2258..2806)
                     /gene="cyaB"
                     /locus_tag="VGE59_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEY1074877.1"
                     /translation="MNEVEVKARAKNPEKLLEALADVGVKFNDPSLQHDTIYVDNDYD
                     PKNPKLDRNILRLRSETGRNLLTLKRDGKNELDSLERETAIADAKSMHDILFFLGYKP
                     FIEVKKYRRKARFNDIELCLDEVENLGMFIEVEKLTQEEDTEKIQEELFSFLLSLGVQ
                     EEDRVTQGYDTLVRLYNEGSMK"
     gene            complement(2852..2925)
                     /locus_tag="VGE59_04260"
     tRNA            complement(2852..2925)
                     /locus_tag="VGE59_04260"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2891..2893),aa:Trp,seq:cca)
     gene            complement(2991..3158)
                     /gene="rpmG"
                     /locus_tag="VGE59_04265"
     CDS             complement(2991..3158)
                     /gene="rpmG"
                     /locus_tag="VGE59_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY1074878.1"
                     /translation="MAKKKGKVQHAVMECGNCRNRNYKTERNVVNVTQKLVLSKYCST
                     CKKHTDHKETK"
     gene            complement(3218..3292)
                     /locus_tag="VGE59_04270"
     tRNA            complement(3218..3292)
                     /locus_tag="VGE59_04270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3258..3260),aa:Thr,seq:ggt)
     gene            complement(3339..3424)
                     /locus_tag="VGE59_04275"
     tRNA            complement(3339..3424)
                     /locus_tag="VGE59_04275"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3388..3390),aa:Tyr,seq:gta)
     gene            complement(3426..3501)
                     /locus_tag="VGE59_04280"
     tRNA            complement(3426..3501)
                     /locus_tag="VGE59_04280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3466..3468),aa:Thr,seq:tgt)
     gene            complement(3593..4381)
                     /locus_tag="VGE59_04285"
     CDS             complement(3593..4381)
                     /locus_tag="VGE59_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1074879.1"
                     /translation="MLYLILTILATVILLALALRRLPYLRTHRLGMEIMSPRPYIGKS
                     SSPFMDIWQRVKSIIGGLSSSVRRPVSLPMPSLTRPNTFQASVSPSGVATLPTSRETD
                     VTDTLPMTAHPGDFWQEDAPQPTGILEIPTKGLITRRGESQKIAHELVIRADESFRKK
                     DYKEAEKFYLQAATKDPDNARIYSRLGVIYLQVKNYKDAVEAFRGALKFDDRVASRHY
                     NLALAYLGKKDYRLAERGLREAVRLDPTNEKYRQTLGAIQKQVD"
     gene            complement(4391..5038)
                     /locus_tag="VGE59_04290"
     CDS             complement(4391..5038)
                     /locus_tag="VGE59_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074880.1"
                     /translation="MSSQSFLSETSESLAHATAIIGASLVERTQLISHLNISPFDTVI
                     LNAAETGGIKETREFANQLLLSPQFGSVRLGLTLEADKLSPQAQNALLKLLEEPPTRV
                     KLILFISREADVLATVLSRCRVYYGSHAQVSEQVGPYLNDPIAQLLDAETLAKDEQVV
                     SRVKGWLASEYASWCQQGRPVGGLDDVLKFWYCYRDIEAGINKRLLLEQLVISSL"
     gene            5139..5531
                     /locus_tag="VGE59_04295"
     CDS             5139..5531
                     /locus_tag="VGE59_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEY1074881.1"
                     /translation="MPYQDQTLTCRDCGKSFVFTARDQEFFAEKGFTNAPTRCKDCRV
                     LKKKAPAGAPAGAGSANKALFKITCKKCGKVGEMATEPRKPEDVLCSECFYDDFKKQL
                     AEQGFPAPKPEQTPTEEETTDGIVPQDE"
     gene            complement(5604..5810)
                     /locus_tag="VGE59_04300"
     CDS             complement(5604..5810)
                     /locus_tag="VGE59_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HEY1074882.1"
                     /translation="MKKTFDRTQLGPMCISCGKTTVMGINKPHSQKRTKRIVKPNIQS
                     YMGLPLCTRCLRSLKEKQAAFVSA"
     gene            5942..6562
                     /locus_tag="VGE59_04305"
     CDS             5942..6562
                     /locus_tag="VGE59_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY1074883.1"
                     /translation="MVFDLLLSNPIHGIIFLLAIGLAIGIHEAAHAWAADKLGDPTAK
                     LMGRTTINPLAHLDLVGTLLFLFAGFGWGKPVPVDERRLKSNQDIVKVALAGPASNLI
                     TAIILGIAYRLIPLTGVQEVLSLFIFINLSLMLFNLIPIPPLDGSKLLKLIVPEPTYD
                     ALVQYGFFLIIILFLMFRIGNLGLGDWLLRGVDILFRFITQATFSL"
     gene            6831..6907
                     /locus_tag="VGE59_04310"
     tRNA            6831..6907
                     /locus_tag="VGE59_04310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6865..6867,aa:Pro,seq:ggg)
     gene            6988..>7620
                     /locus_tag="VGE59_04315"
     CDS             6988..>7620
                     /locus_tag="VGE59_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074884.1"
                     /translation="MKIESVRLIHAQGVPNHYVANQVYQPLDEDHRKRGSFAILLNHA
                     HTTQGAFIASLINTLIREYYRNHTPDLLTSFEQTLVRLNEQIRQYTRSRDTIGAIDGV
                     IALLAGDEVHLTHIGKPLAYLSRGSELLELVETADAKEGETPNFSVITSGEIQTDDVL
                     ILLTNLESTENLKHDIPFSLLQTPLFEAGRAFARIKRGQGERAFESVFIRF"
BASE COUNT         2061 a         1796 c         1569 g         2194 t
ORIGIN      
        1 cgaaaaacag cctttttatt agattgggag atttgatatg atttccctat gaaaaaagta
       61 attgcgggaa tgacaatgtc gctagacgga tacatcaatg accctaatgg aagcatcgaa
      121 aagctttact ccgattttag cgaactcaat aatgccccta gctttcaggg aatgatccat
      181 aataccggtg cagttattat gggaagaaat ctatatgaga tgcaagaccc gtttatgtgg
      241 gcaaatgatg actatgaatt tcaagttcct atctttgttt tgacacatac ccctcccact
      301 aagtatccaa aaggtaacga taacctcggc tttacatttg taaccgatgg cattgagagc
      361 gctctatctc aagcaaaaaa tgcggccggt gacaaagatg tccaaataat cggcggcgca
      421 aacaccattc aacaatacct taataccggt ctatgtgacg aactactgat agatattatg
      481 ccagttcttc ttggtaaagg attatcatta tttgaaaaca taaacactaa taagataact
      541 ctagaaagaa ttaaggtgga agaaactact tccctgagaa caagcctcac ctttaaagtg
      601 acgaatcgaa accatcagaa ctaaactttt aactgttatt ttgctgacag gccggagtgg
      661 aggatgttag cattcacctt tcaaggtacc gtttcgccag ttcttgaacc gtcatcccct
      721 ttgtccaatt agattcaatc cacgcgcgac tttccgcatg cttttcatag cctttatctt
      781 gttcttcgat cacatcattg aattctgtat ttaataatac agttagtgct tctttgaatt
      841 caccatgttt atcttcctct ccctgctcaa taaaatacgg ctgaataagt aaatgcgtgt
      901 aaaagtgcaa cagctcatga atcataacgc cgatagggtt cggtcgcgta aagcatacaa
      961 agaagtatct ctcaggaaaa ttgtacgcac aacgaccatt caacgaaaga taaacggtaa
     1021 tattctcaaa aggcgcagat gtggcaaaca gtttgtccat acgactgatg gccgactggc
     1081 tgattttatc ccactttttc tgaatatcat gggccgcttg ttgactatta atattttcgt
     1141 cttgtatttt ctgggcaata aatttatcta cttcttcttt agaagataaa ttaacctttg
     1201 cagccatcat ctcccgcact aactttgatt gatgctctgg attaaaaaca gttttcaatc
     1261 cacgctgcag attgtcgaat tctttatcta aatcgtaagt aatagtcagt ttcataccag
     1321 cactatacca aaaagcttat aatttcttta tccctggcta acttatcttg attaactgca
     1381 gcctgagtat tggcattaaa ctgaataccg ttttccgaca tccagctata acgatgaatc
     1441 actgcgtaaa cgtcaactaa acgtttttgc tcattagtaa ggtctagagc tttcattact
     1501 tgctcggtat aaaagtctcc atactccgta ccaggccaat tggcacggat tgcgccaata
     1561 gattggatcg ggtcgccgta cttcacacca tctaggtcca cgagaccgtt aaacttacct
     1621 gcatgaatca tgacgttttt actgtttaag tcgtcgtagt aaaaaagaga ctttgcgtta
     1681 tcgaaatacg ttttattgga ttggaaaata tcatttactt tctgccaaag atcttcatca
     1741 aagatccctg tctggcgcat acgttcattg gcacgctgca actcgttttc tatctcaata
     1801 gttaagctat caaccaaatg taattcatcg gcacctaccc atccaaactt gccattcgtc
     1861 ggaagctttt cgagtgtttt tacaatatga gcaatttcgt gcgccagatt agttagttga
     1921 tcatggttta attcagcaat aacgtcgggc agatcctgcc cttctaacat ggaaagcaca
     1981 tggtaaggat acggaataaa cgttcctgta taatcttcgg ctaaaagttc aggaacattg
     2041 atcccctgct gctgaaacat cgggatgtat ttactacttc ctagtaggtt gttatttgtt
     2101 acgccaatcc ggacaattac cgattgatcc ggcaacttgg cttcgtagac ctcattacac
     2161 agccctgtag tttttcgtgt gactacgctg ggttgagtat gaaaatgtcg attaatgatt
     2221 ttgaaaatta actcatcatg catatttgta gagtctctta tttcatactt ccttcattgt
     2281 agagccgtac cagcgtatca tatccttgag taaccctgtc ttcttcctga acacccaagc
     2341 tcaagaggaa actaaataat tcttcctgaa tcttttcggt atcttcttcc tgagtcaact
     2401 tttcaacttc aataaacatt cctaagttct caacttcgtc aaggcaaagt tcgatgtcat
     2461 taaaccttgc tttgcgacgg tactttttta cttcaataaa tggtttatac cctaagaaga
     2521 ataaaatatc gtgcatgctc ttcgcatctg caattgccgt ttctcgttct aaactatcaa
     2581 gttcattttt tccatcgcgc ttaagggtca aaagatttct tcctgtttca cttcgaaggc
     2641 gtaaaatatt tctatctagc ttaggatttt taggatcata atcattatcc acatagattg
     2701 tatcgtgctg taggctagga tcgttaaatt ttacccctac atcagcaagt gcttctaaca
     2761 gtttttctgg attcttagcc ctggctttaa cttcaacttc attcatatta gtactatacc
     2821 aaaaaagcag ccgtaaagct gctttcttgg ttggcagggg tggagggaat cgaacccccg
     2881 ccaacggttt tggagaccgc tgttctacca ctaaactaca cccccgtgta caaaaattat
     2941 tataccttat ttcccctaaa cagaaaatcc cgccggagcg ggaatttctg ttacttggtt
     3001 tctttgtgat cggtatgctt cttgcatgtt gagcagtatt tgctcaatac aagtttctgc
     3061 gtcacgttta caacgtttcg ctcagtcttg tagttacgat tccggcagtt gccgcattcc
     3121 ataacggcgt gctgcacttt tccttttttc ttggccattt tagttccttt ctaagggcaa
     3181 agttgggtac ttcgttcctt ttagaggtgt gaagtcctgg agctgacacg gggatttgaa
     3241 ccccggaccc cttccttacc atggaagtgc tctaccactg agctatgtca gcgttgttgt
     3301 ttacggatac ctttcaagct ttaggctctc tgagtatatg gtgccgagtg agggattcga
     3361 acccccgaag ccgtaaggca gctgatttac agtcagccgt gattgaccac tccaccaact
     3421 cggcgtggag ccgacggagg gaatcgaacc cacgacccgc tgtttacaaa acagccgctc
     3481 tggcctctga gctacgtcgg cgcacaaaaa ttctttacga aagtacaaac tacacagata
     3541 agaataccat aatccccacc tgtcagcaac ccttttagag gcaacggagc ggttaatcga
     3601 cttgtttttg gatcgcgcct aatgtctgac ggtacttttc gttagttgga tcaaggcgaa
     3661 cagcttcgcg gagtccccgt tcggctaaac ggtaatcttt cttccccaaa tacgctaggg
     3721 ccagattata gtgacgactt gccacacgat catcgaattt aagtgcaccg cggaaggctt
     3781 caaccgcatc tttatagttc ttaacttgga ggtaaattac cccgagccgg ctataaatac
     3841 gggcattatc gggatcctta gttgccgctt gaaggtagaa cttttcggct tctttatagt
     3901 cttttttacg aaatgattca tcagcccgaa taacgagctc gtgggcgatt ttttgagatt
     3961 caccccgacg ggtaatgagg cccttagtgg gaatttcgag aattcccgtt ggctgagggg
     4021 catcttcttg ccaaaaatct ccaggatgag cggtcattgg aagcgtatcg gttacatcgg
     4081 tttcccgtga cgtaggaagc gttgcaactc ccgatggcga cacagacgct tgaaatgtgt
     4141 ttggtcgtgt tagagacggc atagggagcg atacaggacg acggaccgag ctcgatagcc
     4201 caccgattat agatttaacg cgctgccata tatccataaa aggagatgaa cttttgccaa
     4261 tgtacggtcg cggactcatg atctccatcc caagtcgatg cgtacgcaaa tacggcaatc
     4321 gtcgcagcgc aagcgctaat aaaatcacag ttgctagaat tgtcagaatc aaataaagca
     4381 ttaggcactc ctagagggag gatatcacaa gctgttcaag aagcaatcgc ttatttatcc
     4441 cggcctcgat gtcgcgataa cagtaccaga attttaaaac gtcgtcgagc ccccccaccg
     4501 gacggccttg ctggcaccag cttgcatact cgcttgctaa ccatcctttt actcgagaaa
     4561 caacttgctc atctttggcc aaagtttcag catcgagcag ctgagcaatc gggtcattga
     4621 ggtacggccc aacttgctca cttacttgag catggcttcc gtaataaact cggcagcgag
     4681 acaaaaccgt agccaaaaca tcggcttcac gagaaataaa aagaattagt tttactcgag
     4741 taggaggttc ttccaggagc tttaagagtg cattttgagc ctgaggacta agcttatcgg
     4801 cttccagagt taaacctagc cgtacactcc caaattgggg cgaaagtaaa agctgattgg
     4861 caaattcccg cgtttctttg attcctcctg tttcggcggc gttaagaatc actgtatcaa
     4921 aaggagaaat attaaggtgc gaaataagct gcgtccgctc cacaaggctg gcaccaataa
     4981 tcgccgtcgc gtgggctaaa ctttcggatg tttctgaaag aaaagattgg gaactcattg
     5041 tagacaccta gtcattatag tatactgaac gtgtcaaacg atactagctt tgtcttctct
     5101 aaaagataca ttctaaaacc aataaattta aggagtacat gccctaccag gatcaaacac
     5161 ttacctgccg cgactgtggt aaatcattcg tctttaccgc gcgggatcaa gaattcttcg
     5221 ccgaaaaagg attcactaac gcaccaactc ggtgtaaaga ttgtcgcgtt cttaaaaaga
     5281 aagcccctgc cggagcacct gctggcgctg gatccgctaa caaagcgctc tttaaaataa
     5341 cctgcaaaaa atgcggtaaa gtcggagaga tggcaaccga acctcgcaag cctgaagatg
     5401 ttttgtgcag cgagtgtttt tacgacgatt tcaaaaagca actggctgaa cagggttttc
     5461 ccgcccctaa accagagcaa acccctaccg aagaagaaac cactgacggc attgttcctc
     5521 aagacgaata accgatcaga cactaaaaga ccccggaaat gtatccgggg tcttttagtt
     5581 tatacggaaa atataattgc gtattacgca gaaacaaatg ccgcttgctt ctctttaaga
     5641 gagcgaagac agcgggtgca caaaggcaag cccatgtacg actgaatgtt gggctttacg
     5701 attcgcttcg tgcgcttctg tgagtgaggt ttattaatcc ccattacagt tgttttgccg
     5761 cacgaaatgc acattgggcc aagttgagta cgatcgaagg ttttcttcat cacaattttc
     5821 ctttactacc tgatcatggt atcataattt ttgattcgag acaagcgttt cttaggcctt
     5881 ctatctctaa cggtggtcta tttgattcgt cgatatgcaa taactgaaag tgacatcctc
     5941 tatggttttt gaccttcttc tctccaaccc tatccatggc attattttcc ttttagcaat
     6001 cggcctagca attggtattc acgaagccgc gcacgcatgg gctgccgata aacttggcga
     6061 ccctaccgca aagcttatgg gccgaacaac cattaatcct ttagcccatt tagatttggt
     6121 tggtacgtta cttttcctct ttgcgggatt tggctgggga aagcccgttc ccgtcgacga
     6181 aaggcgcctt aaatccaacc aagatattgt gaaagttgcg ttagccggtc ccgcgagcaa
     6241 ccttattacc gcaatcattc ttggaattgc ctatcgcctt attcctctta ccggagtgca
     6301 agaagtatta agtctgttta tttttattaa cttaagcctt atgcttttca accttatccc
     6361 cattcccccg cttgatggat caaaacttct caaactcata gtgccagaac caacctacga
     6421 tgcattagtt caatacggat tttttcttat cattatactt ttcctcatgt tccggattgg
     6481 aaatttgggc ttaggagatt ggctcttacg aggcgtcgac attcttttcc ggttcataac
     6541 acaagcaact ttctccctgt aagcgcattt cttcggtctc aagaatgtgc tattattggt
     6601 tcaagctcct ttgctcggtt acattttggc ttactaatat tattgggcct atgaatctct
     6661 tcggaggttc agggatccag gcttgcctgg ataaaggttc ggccttctgg cttcattccg
     6721 ctccggcgaa atgagggttc ctcccgcccg tgggtgtgag gacaggactc ccacctagaa
     6781 actagcccaa ttatgtaacc gggcgaggag cgtaaaattc ttcagtgtat cgggacgtag
     6841 catagcggcc tagtgcgccg ggctgggggt ccggagatcg acggttcaaa tccgtccgtc
     6901 ccgaccagaa gatattcgtt tactccgttt taatacgaga gcaacttcga tcgtttccca
     6961 ggcgatgaag ttaaggagga tggggcaatg aaaattgagt ccgtgcgtct catacacgca
     7021 cagggtgtcc ccaatcatta cgttgcgaat caggtttatc aaccgctcga cgaagatcac
     7081 cgcaaacgag gcagttttgc gatccttttg aatcatgccc acacaaccca gggtgctttt
     7141 attgcatcac ttattaatac attgattcgt gaatactacc gaaaccacac gcccgacctt
     7201 ttaacttctt ttgaacaaac cctcgttcgg ttgaacgaac agatccgtca atacacacgc
     7261 agccgtgata ccattggcgc catcgacggt gtaattgccc ttttagctgg tgacgaagtc
     7321 caccttaccc atattgggaa acccctcgcc tatctgtcac gtggcagcga gctcctcgaa
     7381 cttgttgaaa cggctgacgc aaaagaagga gaaacaccta acttctcggt tatcacatcg
     7441 ggggaaattc aaactgatga tgtactaatt ttacttacca acctggaatc gacagagaac
     7501 cttaaacacg acattccttt ttccctccta caaaccccgc tctttgaagc aggacgagca
     7561 tttgcccgta ttaaacgcgg acaaggtgaa cgggcattcg aaagcgtctt tatccgtttt
//
LOCUS       DASWYB010000074         9191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_247135, whole genome shotgun sequence.
ACCESSION   DASWYB010000074 DASWYB010000000
VERSION     DASWYB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247135"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..398
                     /locus_tag="VGE59_04320"
     CDS             141..398
                     /locus_tag="VGE59_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074885.1"
                     /translation="MKSLMVSLVSAVFVCISFITPVLAHGNGDGHDDIPAAGPIGMSG
                     PADLPLWADILVIGGLLLVFIVGVFILLYFHSDTYRKGIHA"
     gene            395..1282
                     /locus_tag="VGE59_04325"
     CDS             395..1282
                     /locus_tag="VGE59_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY1074886.1"
                     /translation="MSHDHSHNHHDSTGNIKTAFFLNLVFTLIEIVGGLFTNSLAILS
                     DALHDLGDTFSLGLSWYLDRVSKKKHDNVYSYGYKRFSLLAAVINSIVLVVGSIYILT
                     EALPRLMNPEHSNAQGMFVLALVGIGINGLAVLRLRSGKSMNERVVTWHLLEDVLGWV
                     AVLIVSIVMYFWDLHILDPLLSLGITLYVLWNVVKNLRKTVKIFLQSVPDSVDTESLE
                     QSITALSKVQAVHDTHIWSLDGEYNVLTTHVAVNKDTSQEEVYDLKCEIKKIAHEGNI
                     EHVTIEVEYEGEDCTTSQH"
     gene            complement(1286..2002)
                     /locus_tag="VGE59_04330"
     CDS             complement(1286..2002)
                     /locus_tag="VGE59_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1074887.1"
                     /translation="MRAITVHNVSKIYGKGHAAVKAVQDVTLAIEPGEIVLIMGPSGS
                     GKTTLLSMIGTLLRPTEGSIVVGDLATRSLNDNQLADLRLESIGFIFQSFNLLSALTA
                     KQNVMVPLIAGGAPKREAGKRAETLLTRLGLGERINSLPADLSGGEKQRVAIARALIN
                     EPKIILADEPTANLDSETGHEVATLLCEIACNENRAVVIVSHDPRLRDVAKRIYTIED
                     GKLIKEEAGGHNKICPVRHT"
     gene            complement(1999..3096)
                     /locus_tag="VGE59_04335"
     CDS             complement(1999..3096)
                     /locus_tag="VGE59_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1074888.1"
                     /translation="MFLIASRNLLQEKTRLLISVIGVAFSVILIVFLVGVYQGLNIEL
                     GKYIATIPSDLWVAQEGSKNLYDSTSVLPAAAQPDIEAVEGVASVKPFQGRQAAVEVN
                     GKEYRAFVVATGEGSPAQPPNIVEGTADMRSGEIILDRVVTGAKLGQEVTVGGKPFTV
                     AGITEGGNVLIVSYAFVMAEDAVRLFRSSEVVNYFVVETEDGADTSAVAAAIESAVPD
                     SKVRGVSEFVENSISVVKEVFLPIISILSLIGGIVGMTVIGLTIYTSVLEKRREYGVL
                     KALGIRNGQLYKIVLQQSLLTSVLGYAVGGLMAFGLMSLATRLAPAFIAEVRPIDMGW
                     LFVATLLMGGLAAFLPARRLTKIDPAEVFKS"
     gene            complement(3121..4068)
                     /locus_tag="VGE59_04340"
     CDS             complement(3121..4068)
                     /locus_tag="VGE59_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY1074889.1"
                     /translation="MYLEALAKIQAVIFDKTGTLTLGKPSVVAIIPQGNYSEKEVLRL
                     AASAEEKSSHPLAQAIVQKAKEQKISLAHAEIKEMSGKGVLARFEGKEILVGKLAFLT
                     EQKVKVVAVEESAYTQIGVAVDKQFIGFLHVADTKRPEAREALEQLKKMGIKDTVMLT
                     GDQEQVAAQIAGEIGITKYYANLLPQDKEKIAKQLRQQYGAVIMVGDGINDAPVLATS
                     NVGIAIGTAGNDIALEAADVALMGSDLRAVPYLIRLGKKAVMKLRTNLVIALGLKLLM
                     ILLGVFGLIPLWVAVLGDDGATLLIIANALPLLRYKLKV"
     gene            complement(4058..5221)
                     /locus_tag="VGE59_04345"
     CDS             complement(4058..5221)
                     /locus_tag="VGE59_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY1074890.1"
                     /translation="MQEPQTTTLYIAGMDCASEVQDVENALKYDGILGHEISYLTQKA
                     VVTYDPAKLTPETIVARIEKQTGYKTKLFKERAKKDEVKEGNKFAKPILVSSAVLLAL
                     GLAGPFLGIAELIAAILLGGSLVIVELPILAKALKRFKSNPFNADILMALAGIGSFLI
                     GHYSEGAAVLLLYTLAELLEDYTVDRVRQTTKRLAELLPQRVLLKVGNTSKEVMVEDL
                     KVSDVILVKPGWRLPIDGVIVSGQSTLDQASVTGESVPVEKNVGDEVLSGTLNIDGSL
                     EVKVTRPFYDSTTNRIIDLVMEAQGRKANIEKFVDRFSRYYTPTMLVLTILVALIPPL
                     LFNQPFSTWFYRSLVVLIIACPSAFLISTPITVLVGLTAAMRSGILIKGGYLP"
     gene            5271..5597
                     /locus_tag="VGE59_04350"
     CDS             5271..5597
                     /locus_tag="VGE59_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY1074891.1"
                     /translation="MYTTMDKDQLQLETKIFKGLGDQSRLHILHVLAEEPKTVSDIVE
                     VTQLSQPNVSMHLACLLDCGLVKREKQGRQVYYRTATAEIEAILASARTILQEHAKEI
                     YDCTRY"
     gene            5578..6186
                     /locus_tag="VGE59_04355"
     CDS             5578..6186
                     /locus_tag="VGE59_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY1074892.1"
                     /translation="MTAPVTDRASLIRKALLLAYFIIAWDVIEGMVAVTAGILANSVA
                     LIGFGIDSGIEVFAAIVTAWYLRGNGQRDKKLPLRLIAVSFFVLAAYVGFKAISDLLT
                     GSQAEPSTIGIVWSFVALAIMVPIALVQRKTGIALNNKVIISQADQTWLSNYLSLSLL
                     IGLGVNALWGWWWADPIVALVLAFYSISEGVEAWEEAEKLET"
     gene            complement(6191..7540)
                     /locus_tag="VGE59_04360"
     CDS             complement(6191..7540)
                     /locus_tag="VGE59_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074893.1"
                     /translation="MSQLLFATIFTFLFPAIASAHGIGEVYKLPVPLHYYLAGAGVAV
                     AVSFFALGLFFNKPSSGKELNRIVHVPWLSSVLKGGRVLAVLLLLLTIATGAFGADDL
                     SKNFAPTFFWVYFLIGFSILSALIGNLWDKLNPWKTLAYATGLPRKTGKQVSGWVGVV
                     LLLGLFWLELVSGKSFIPSTVGLALLGYSWFAIVFSRFYDNWYTEGELFSVLYGFIGK
                     LAFVRISDDNTSLILTNPYKGTKAATAHWWTLGLASVLLAGASFDSLKESTLWFDWIQ
                     ALGFTGSTYKIADTLGLILAPIPFYALYLAAIGIMRKLMGNTYDFKTLAYRFVWSLIP
                     IAFGYTLAHNFSLTIVAAPSMLAFLSDPFGFGWNLFGTADFAQTNLLLGAKAVWFTEI
                     GFIVAAHIAGVWFAHVVAMHLFPNTQQATKSQYPMLLLMVGYTVMTLWLLSQPIVVR"
     gene            complement(7542..7979)
                     /locus_tag="VGE59_04365"
     CDS             complement(7542..7979)
                     /locus_tag="VGE59_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074894.1"
                     /translation="MKNSSAIFIAIGVLVLLAAIFFFNKSANPEPAVTATPTGQQSTT
                     ASSEASPIPSPTPSVAELIINVPADLTLTAQTLRVKEGEKATIKVTSQVADEVHLHGY
                     DLSQAVGPGTPAQIDLTADKTGRFEIELEERGKAIGILEVYPK"
     gene            complement(7987..8505)
                     /locus_tag="VGE59_04370"
     CDS             complement(7987..8505)
                     /locus_tag="VGE59_04370"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY1074895.1"
                     /translation="MDKGVLLIGGITVAVLAIIIGITSADPSAKYAGKEAVGEEVALL
                     EAKHIPDGSPRPEYNSNPPTSGSHYIQPAPWGIKTEQIQDETLVHNLEHGGVWITYNP
                     QTASQEIVDKLTEIARGYRSKVVLSPRVQNDSVIALASWGKISKLDSVDEAKIQEFIG
                     RNKNKGPEQVPD"
     gene            complement(8563..>9191)
                     /locus_tag="VGE59_04375"
     CDS             complement(8563..>9191)
                     /locus_tag="VGE59_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074896.1"
                     /translation="LEMKQTKKLIKDKYDIVMPDTETARLVTLIKELEWWQKASAKSD
                     SPRQFREEQLDDLRELLEQSGGQNLSRLDVRNHATSLLALVPMKEKQRVADEIRGILV
                     EHRPVAYEPVVAKKTAELLKIHYDVTLTDEQLEQVIPFLTRKLWYEVGLDSSLEECLD
                     DLILYANKRKRGERVKDAILHDAIRQALDLTVSKFTEQRIDPLYNQQV"
BASE COUNT         2404 a         2179 c         2157 g         2451 t
ORIGIN      
        1 gagaaacctc acccgggtgc tgatactgaa gtaacctaca ctgttactcg tgacggtcaa
       61 gaagtgaata agcaagtctt taaatccaag taccaagcat ggcaagctcg tttcttagtt
      121 ggcaccaagg agtaagcagc atgaaaagcc tcatggtttc cttagtaagt gcagtgttcg
      181 tttgtattag cttcataact cctgttttgg cccacggaaa cggtgatggt catgacgata
      241 tcccagcagc ggggccgata ggtatgtcag gtcctgctga cttacccctg tgggcagata
      301 ttctagttat cggtggctta ctacttgttt ttattgttgg ggtgtttatt cttctctatt
      361 ttcacagtga cacctaccgg aaaggaattc acgcatgagc cacgatcact cgcataacca
      421 tcacgatagt accggtaaca taaagacagc tttctttctc aacttagtat ttaccctcat
      481 tgagattgtg ggtggccttt ttaccaatag cttagcaatt ctttccgatg cgcttcatga
      541 cttgggcgat acgttttcac tgggactatc ttggtattta gatcgggttt ccaagaaaaa
      601 gcacgataac gtctactcct atggctataa acgcttttca ttactagcag ccgttattaa
      661 cagtatcgtt ttagtggttg gatcaatcta tatcctaacc gaggcgctcc ctcgactcat
      721 gaatcctgaa cactccaacg cccaaggcat gtttgtcctt gctctcgtcg ggataggcat
      781 taatggcctg gcagtcctgc gactacgaag cggcaagagc atgaacgaac gggtagtgac
      841 gtggcatttg ctcgaagatg tgctcggttg ggtcgcggtg cttatcgtca gtattgttat
      901 gtatttctgg gatcttcata ttttagatcc gttactttct ctgggcatca ctctgtatgt
      961 actctggaat gtggtcaaga atctccgtaa aacagtaaag atatttctcc aaagcgtacc
     1021 ggattcagtt gacactgaga gcttagagca aagcataacg gcattatcaa aggttcaggc
     1081 tgtccacgat acccatattt ggtccttgga cggcgagtat aatgtgctta ccactcatgt
     1141 ggcagttaat aaggacacat cgcaagaaga agtttatgat ctaaaatgcg aaatcaaaaa
     1201 aatagctcat gagggcaata ttgaacatgt cacaattgaa gttgagtatg agggcgagga
     1261 ttgcactaca tcccaacatt gatttttacg tatgcctcac gggacatatt ttattatgac
     1321 cacctgcttc ttcttttata agctttccat cttcaatggt ataaattctc ttggcgacat
     1381 cacgtagacg gggatcgtga ctgacaatca cgactgctcg attttcgttg caggcaatct
     1441 cacataacag cgtagcgact tcatggccag tctctgagtc aaggttagcc gtcggttcat
     1501 cagctaggat aatcttgggc tcattaatga gcgcgcgtgc aatagcgaca cgctgttttt
     1561 cgcctccaga taggtctgcc ggaaggctat ttatacgctc acccaatcct aagcgagtaa
     1621 ggagggtctc tgcccgcttc ccagcctcgc gcttcggtgc accgccggct atcagaggca
     1681 ccatgacgtt ctgcttcgca gtgagagccg acagcagatt gaagctttgg aagataaagc
     1741 caatggattc cagccgtaag tcagccagct ggttatcatt taaggagcga gtggcaagat
     1801 caccaacaac aattgaccct tcggttggtc taaggagcgt gccgatcatc gataataggg
     1861 tggtcttacc tgatcctgaa ggacccataa ttaagactat ctccccaggt tcaatggcaa
     1921 gggtgacatc ttggaccgct ttgacggcag cgtgaccttt gccatagatt ttgctaacgt
     1981 tgtggacagt aatagctctc atgatttaaa tacctccgct ggatcaatct tcgtgagtct
     2041 tcgtgccggt agaaaggcgg caagcccgcc catcaggagg gtagctacga agagccaccc
     2101 catatcaatt ggacgaactt cggcgatgaa cgcgggagct agtctcgtag caaggctcat
     2161 caggccgaat gccataagtc caccgacagc atatcccagg acgctagtga gaagggactg
     2221 ctgtaaaact atcttgtaca gctgaccgtt gcggataccc agcgccttca agaccccgta
     2281 ctcccgccgc ttttccagga ccgaggtgta gatagtgagg cctatcacgg tcatgccgac
     2341 gatgccgcca atcagggaaa gtatggaaat tatcggcagg aagacttcct tgacgacgga
     2401 gatggagttc tcaacgaact cgcttacgcc ccggacctta ctgtcgggca ccgccgattc
     2461 gatggcggca gcgacggctg aggtatcggc accatcttcg gtctccacga cgaagtagtt
     2521 gaccacctcc gaggagcgga ataggcgcac cgcgtcttcc gccatgacga aggcatagga
     2581 gacaattaat acattgccac cctcggtgat tccagctacg gtaaatggct tgccgccgac
     2641 tgttacctcc tggccgagct tggcgcctgt taccactcgg tctaagatta tctcgccgct
     2701 tcgcatgtca gccgtccctt ccacgatatt cggcggctgg gcgggagaac cctcgccagt
     2761 ggcgacgaca aatgcccggt attccttacc attaacctcg actgccgctt gcctgccctg
     2821 gaacggcttg actgacgcca cgccttcgac cgcctcaatg tctggctggg ctgcagccgg
     2881 caggacagat gtgctgtcgt agaggttctt gcttccttcc tgggctaccc acaggtcaga
     2941 cggaatggtc gcaatatact tgccaagctc aatgttcaac ccctggtaga ctcccacaag
     3001 aaagacgata aggataacac tgaaggccac gccaatcaca ctaatcaaga ggcgggtttt
     3061 ttcttggagt aagtttcggc tagctataag aaacatgatg atacctcctt agtttgggga
     3121 ttaaaccttc agtttatacc gcagcagcgg caaggcattg gcaatgatca acaaggtagc
     3181 gccatcatct cctagtacag ctacccataa cggaataagg ccaaagacac caagtagaat
     3241 catgagaagc tttaagccta gagcaattac taagttcgtt cgaagcttca taacggcttt
     3301 cttgccaagt cgaatcaagt aaggaacggc tcgtaaatca gaacccatta aggctacatc
     3361 ggctgcctct aaggcaatat cattgccggc tgtaccaata gcgataccga cgttacttgt
     3421 tgcgagaacc ggcgcatcat tgataccgtc tccgaccata atgacagcgc cgtactgttg
     3481 tcgaagctgc tttgcaattt tttctttatc ttgagggagt aagttcgcgt aatatttggt
     3541 tatgccaatt tcgccagcaa tttgagccgc cacctgctct tggtctccgg tcagcatcac
     3601 cgtatctttt atgcccatct tttttaactg ctcgagtgct tcgcgggctt ccggccgttt
     3661 agtatcggca acatgaagaa aaccgataaa ctgcttgtcg actgcgactc ctatttgagt
     3721 gtaggcactc tcttcgacgg cgacaacttt aaccttttgt tcggtaagga atgccaattt
     3781 cccgacaagg atttctttgc cttcaaaacg agcaagcacg cccttacctg acatttcctt
     3841 aatctcagcg tgagctaagg agattttctg ctcctttgcc ttttgcacaa ttgcttgcgc
     3901 taaggggtgg gaagattttt cctcagcact agccgccagc cgcaagactt ctttttcact
     3961 gtaattaccc tgtggaatga ttgctacaac cgagggcttc cccagggtaa gagttccggt
     4021 tttatcgaag atgacagcct ggattttggc taatgcttca aggtaaatac ccccccttaa
     4081 ttaagatgcc gctgcgcatg gccgctgtta ggccgacaag aacggtgatt ggggttgaaa
     4141 tgagaaaagc actggggcag gcaatgatta atacaaccaa tgaacgatag aaccaggttg
     4201 aaaacggctg attgaagagg agcggtggga tcaaggcaac gagaatagtt aagaccagca
     4261 tggtcggcgt ataatagcga gaaaaacgat ctacaaactt ttcaatatta gctttgcgtc
     4321 cttgagcttc cattaccaaa tcaatgatgc ggtttgtcgt tgagtcgtaa aacggccggg
     4381 ttaccttaac ttcgagcgag ccgtcaatat ttaatgtacc ggataggact tcatccccca
     4441 cattcttttc tacaggaact gattcgccgg tcaccgaggc ttgatcgagg gttgattggc
     4501 cgctgacgat aacgccgtca ataggcaacc gccaaccggg ctttactaag ataacatcgc
     4561 tgacttttaa atcttcaacc atgacttctt tgctggtatt gcctacttta agtaggacgc
     4621 gttgaggcaa gagctcagcg agccttttcg tcgtttgccg tactcggtcg acggtatagt
     4681 cttcaagcag ctctgccagg gtatagagaa gtagtactgc cgctccttcg ctatagtggc
     4741 cgatcagaaa cgagccgatc ccggctaaag ccatcaaaat atcggcattg aaaggatttg
     4801 atttaaaacg ttttaatgct ttagcaagaa ttggcagctc aacaataaca agcgaaccac
     4861 caagtaaaat agcagcaatt aattctgcta taccaagaaa cggtccagca aggccaagag
     4921 caagcaaaac cgcactgcta acaagaatag gcttggcgaa tttattgcct tccttcacct
     4981 catctttttt agctctttct ttaaagagct ttgttttata accagtctgt ttctcgatgc
     5041 gagcaacgat cgtctccggc gttaactttg ccgggtcata cgtaacaact gccttttgtg
     5101 tcaggtagct gatctcgtga ccgagaatgc cgtcatattt taaggcattt tcaacatcct
     5161 gcacttcgga ggcgcaatcc ataccagcga tatagagggt ggttgtctgt ggttcttgca
     5221 tacatacctc atctagggtt tatattgaat atatcagcgt ataatgatat atgtatacta
     5281 ctatggacaa agaccaatta caacttgaga ctaagatctt caaagggctg ggcgatcaat
     5341 cacggctgca tatcttgcac gtacttgctg aagaaccaaa aacggtttca gacattgttg
     5401 aggtgacgca gctttcgcag ccgaatgttt ccatgcactt agcctgtctg cttgattgcg
     5461 gactcgtcaa acgagagaaa cagggccgac aagtctatta tcgtactgct acagccgaga
     5521 tagaagcaat tcttgcttca gcccggacaa ttttacaaga acacgccaag gagatttatg
     5581 actgcacccg ttactgatag agcttccctc attcggaagg cgttgctcct tgcctatttc
     5641 attatcgctt gggatgtcat cgagggcatg gtagcggtta cagccggtat tttggctaat
     5701 tccgtcgccc ttatcggctt tgggatagat tctggcattg aggtcttcgc tgcgatcgta
     5761 acggcttggt atttgcgagg caacggtcaa cgagacaaaa aactaccact gcgtctcatt
     5821 gccgtaagtt tctttgtcct tgccgcttac gttgggttta aagcaattag tgatctctta
     5881 accggaagcc aggcagagcc atcaactatc gggatcgtct ggagctttgt tgctcttgct
     5941 atcatggtac cgatcgctct tgtgcagagg aaaaccggca tagcactgaa taacaaggtt
     6001 attatcagcc aagctgatca gacatggtta tccaattatc tttcactgag cctgctgata
     6061 ggcttaggcg ttaatgctct ctggggttgg tggtgggctg acccaatcgt agccttagta
     6121 cttgctttct atagtatatc tgaaggtgtt gaagcctggg aagaagctga aaagctagag
     6181 acatagactg ctaacgtacg acaataggtt gagataaaag ccataaggtc attaccgtat
     6241 agccaaccat aagcaaaagc ataggatact ggctcttcgt tgcttgttgc gtattaggaa
     6301 agagatgcat cgcgacaaca tgggcaaacc agacgccagc aatgtgagcc gcgacaataa
     6361 agccaatctc agtaaaccag actgctttcg cgccaagtaa gagattcgtt tgagcaaagt
     6421 cagccgtgcc aaaaaggttc cagccaaacc caaacggatc agaaaggaac gcaagcattg
     6481 agggagcagc cacaatggtt aatgagaagt tatgcgccag cgtatagccg aaggcgattg
     6541 ggatgagcga ccacacgaaa cggtaggcca gggttttaaa gtcatacgta ttgcccatta
     6601 gttttcgcat aatcccaatc gctgcgagat agagtgcata gaatgggatt ggtgccagta
     6661 tcagaccaag cgtatcagca attttatatg ttgacccggt gaaaccgaga gcttgaatcc
     6721 agtcaaacca aagcgtgctt tctttcaaac tatcaaaact agcaccggct agtaggacgc
     6781 ttgccagtcc aagcgtccac caatgagccg tggcagcttt tgttcctttg taagggttag
     6841 tgagaataag agaggtgtta tcgtcgctta tacgaacaaa agcaagcttc ccaataaaac
     6901 cgtagagaac ggagaacaac tcaccttccg tataccaatt gtcgtagaaa cgagaaaaga
     6961 caatcgcaaa ccagctatat cccaggagag cgagtcctac cgtcgaaggt ataaatgatt
     7021 ttcctgatac cagttccaac caaaataatc ctaagagcag cacgacccca acccagccag
     7081 aaacttgttt gcctgttttc cttggtaagc cggtagcata tgccagcgtc ttccaaggat
     7141 taagcttgtc ccagaggttt ccgatcagtg cacttaagat actaaagcca attaaaaagt
     7201 agacccagaa aaaggtcgga gcaaagttct tagataggtc atctgcgcca aacgcgcctg
     7261 tagcaattgt taacaggagg agaaggacgg ccaatactcg tccaccttta agtacggacg
     7321 acagccaagg tacatgaacg atccgattga gctcttttcc gcttgatggt ttattgaaaa
     7381 ataagccaag ggcaaagaaa gacaccgcca ccgccacacc agcaccagct aaatagtagt
     7441 gaagcgggac gggtagttta tacacttcac caatcccgtg ggctgaagca atagcgggaa
     7501 ataagaacgt gaagatagtg gcgaacagaa gttgactcat actatttcgg atacacttcg
     7561 aggataccaa tggccttacc tctttcttcc aattcgattt caaaacggcc ggttttatcg
     7621 gcggtaagat ctatctgcgc aggtgtacct ggtcctactg cttggcttaa atcgtaacca
     7681 tgcaaatgaa cttcgtcggc tacttgtgag gttactttaa ttgttgcttt ttcgccttct
     7741 ttgacacgta acgtctgagc tgtaagtgtt aagtctgctg ggacattgat aataagttcc
     7801 gcaacgctag gcgttggaga aggtataggc gatgcttcgc tgctcgcagt agtcgactgc
     7861 tgtcccgtcg gcgttgctgt aacggcaggc tcgggatttg ctgacttatt aaaaaagaaa
     7921 attgctgcga ggagcactag tactccgatc gcaataaata tggctgaaga atttttcatg
     7981 cgactcctaa tcgggaactt gttcagggcc tttattctta ttgcggccaa taaattcttg
     8041 aatctttgct tcatcaacgc tatctaactt agagatttta ccccaagacg ctaaagcaat
     8101 aacgctatcg ttttggactc gaggcgacag tacaacctta ctgcgatatc cgcgtgcaat
     8161 ctcagtgagt ttatctacga tctcttgtga cgcggtttgt ggattgtacg taatccaaac
     8221 gccaccatgc tcgaggttgt gtacgagtgt ttcgtcttga atctgctcgg tttttattcc
     8281 ccaaggggca ggctgtatgt agtgagaacc agatgtaggt gggttagagt tgtattccgg
     8341 ccggggcgaa ccgtcaggaa tgtgttttgc ctcaagcaaa gcaacttctt cgccaactgc
     8401 ttctttaccc gcgtattttg cactggggtc tgccgaagtg attccgatga taatagccag
     8461 tactgcgact gttattccac cgattaggag tacgccttta tccatataaa tcctcacatt
     8521 taatactaag cttatacgtt actatattac cataaaatcg catcaaacct gttgattgta
     8581 gagtgggtct atcctttgct ctgtgaactt tgaaactgtc aaatcaagag cttgtcggat
     8641 cgcatcatgt aatatggcgt cttttactcg ttcgccacgc tttcgtttat tggcataaag
     8701 tataaggtcg tctaagcact cttcaagtga gctatcgagt cctacttcgt accagagttt
     8761 tctggttaag aatgggatga cttgttcaag ctgctcatcg gtaagtgtta catcgtaatg
     8821 aattttaagt aattcagctg tcttcttggc tactacgggc tcgtaagcca cgggtcgatg
     8881 ttctacaaga ataccgcgta tctcatcggc tacccgttgc ttttccttca ttggcacaag
     8941 agccaaaaga gatgttgcat ggttgcgaac atctaatcga gataaattct gaccaccaga
     9001 ctgctctaag agttctcgta aatcgtcgag ctgctcttct ctgaattgcc gaggtgagtc
     9061 tgattttgcg ctagcctttt gccaccactc aagttctttg atcaatgtaa caagccgagc
     9121 tgtttcagta tcgggcatca caatatcgta cttatctttg ataagtttct tggtctgttt
     9181 catctctaat t
//
LOCUS       DASWYB010000075        13535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_93705, whole genome shotgun sequence.
ACCESSION   DASWYB010000075 DASWYB010000000
VERSION     DASWYB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93705"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VGE59_04380"
     CDS             <1..265
                     /locus_tag="VGE59_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074897.1"
                     /translation="ILQGHARAYVTVGRRVETRQFTLPLRTPREAVKSLFAGVRHDCP
                     GANQVQVVALTLELPTGETLQDLEDPSRYLSAFPYLATVWPIF"
     gene            351..421
                     /locus_tag="VGE59_04385"
     tRNA            351..421
                     /locus_tag="VGE59_04385"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(474..550)
                     /locus_tag="VGE59_04390"
     tRNA            complement(474..550)
                     /locus_tag="VGE59_04390"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(514..516),aa:Ile,seq:gat)
     gene            complement(692..1723)
                     /locus_tag="VGE59_04395"
     CDS             complement(692..1723)
                     /locus_tag="VGE59_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY1074898.1"
                     /translation="MKFLLGTVRPISLLAWIPVVAGACLAGSLGWGVLYGSIGAILSV
                     GWINAFNNVIDRHIDRLNPAKKTLAATNPVKAYEVALYLLPGITVMLRQLEYNNVLLA
                     MLFYLGYWYSYLFGRVPYLKRIVVATCVAGTSFMLAAQYPPQFWMWVMSAFAFIFIRE
                     SGKDKADRVEDKQFRFVYPQKIDWWCITAPVLGAVLFLGCLIAAGQPIGLDDIALATG
                     VTIAIWSYIQIRVVYGQYHASFRHRTPAGRMGMVVALVGLMPAFVSTPLLLVVWINSV
                     SILYRSYLPSRFARQLMAIVHDAALWGTLPIIAACRIGTLAPSLVIAAGILFTGTLAN
                     QLMRRRVVV"
     gene            1857..2456
                     /locus_tag="VGE59_04400"
     CDS             1857..2456
                     /locus_tag="VGE59_04400"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1074899.1"
                     /translation="MHTVDFYKGVEGKKQAAFVILYALDGMPWEEHPKLDRHGLVVPT
                     CLMMNRWDNWCGFIGGKVDPGETLEQAAIREVEEEIGHTLTLSLEPIVAHDFGPLTTH
                     AFAAQVTYQELRKLQEQASHSPHFGSEVTGVFLPHLVDYEQKNITQDSSMVSLLKSSF
                     APTVREEMVHFLLKKGIFRKEDLAGICDRAGFSLDQLLQ"
     gene            complement(2531..3010)
                     /locus_tag="VGE59_04405"
     CDS             complement(2531..3010)
                     /locus_tag="VGE59_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074900.1"
                     /translation="MALDDLLREKIGLAFRVVQQVNPAVNMGIIEQQFFEALNPLVDD
                     ESHPAVLEPYRVVLQGVKDSEIRQIRVYDRHGGLAAIRCFRQLRMGAHPILVWMLREE
                     NRNRPHPWRTLAEKSGMYASPGNVNGHLKPLKLPCLISLGEKSELQWMISVSEVIWE"
     gene            3091..3591
                     /locus_tag="VGE59_04410"
     CDS             3091..3591
                     /locus_tag="VGE59_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074901.1"
                     /translation="MITIAHVLVGGAVGVVTGNPLAALVAGTASHFAMDLIPHLDHPP
                     YAPKDKKGDVIFSQAIYVQAFCDVIIAATIVCILWYSWFDFSTLHPFAWGAFGGFLPD
                     LIDNVPFWNKPFRKSWFGSRFHRFHDAIHHVWTKTFPMHRYWLLGILTQVVAVVIAWN
                     VLKNYV"
     gene            3704..3779
                     /locus_tag="VGE59_04415"
     tRNA            3704..3779
                     /locus_tag="VGE59_04415"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3737..3739,aa:Ala,seq:tgc)
     gene            3873..3948
                     /locus_tag="VGE59_04420"
     tRNA            3873..3948
                     /locus_tag="VGE59_04420"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3906..3908,aa:Ala,seq:cgc)
     gene            4122..4406
                     /locus_tag="VGE59_04425"
     CDS             4122..4406
                     /locus_tag="VGE59_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074902.1"
                     /translation="MAENLYQLTKRETGERVGLPRERGIVVREAMRLINKPSGFDIIT
                     YPDQWEEGTGEVHVGTRAPGEDALPVGELDAYEYVIRPATADDLAAASAD"
     gene            4670..4864
                     /locus_tag="VGE59_04430"
     CDS             4670..4864
                     /locus_tag="VGE59_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074903.1"
                     /translation="MNNSIVAGFTRQQFSWILMFFEGGGVFTAVLWNPQANQVFITSS
                     ETPLRVVTVSTNKEHYLRHL"
     gene            4890..5510
                     /locus_tag="VGE59_04435"
     CDS             4890..5510
                     /locus_tag="VGE59_04435"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1074904.1"
                     /translation="MVEYRGYEWRVLPGVFAPYPAFSSKKGLSGKNIDMYLGRRVLDL
                     GCGCGVRGIMAGLCGAKEVIATDICEVACRNTLLNIQRHRTFRVKVVCTDMFSGLRGS
                     FDTIVAYLPSRDAAVSKPWERAIYDPGLRLNRQLFDEAWQYLVPGGTLHASLLDQGAI
                     PEFLDQIASRGYELTSHKVCPHETGDWHPLSLRWPVVVTPKEGNHG"
     gene            5503..6132
                     /locus_tag="VGE59_04440"
     CDS             5503..6132
                     /locus_tag="VGE59_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074905.1"
                     /translation="MVNSLLRPFLPLGPEVVVTVMQRLGAICFPIMSEADALALAVEA
                     KRFKYKKQPKEYGKRKVRQEVSSCLVSEGAIGDFASSLERELAVFFGPEAFESPLSFT
                     ARNIQRYRNGTLGIEPHRDESENRNIVALLGLEGRGRFTVYDELDGPVRVRWTINPGD
                     LLLMVAPGFYGADASCRRVHRVDEIVGYPHRYLLGLRQYVPSASRQGEK"
     gene            6132..6548
                     /locus_tag="VGE59_04445"
     CDS             6132..6548
                     /locus_tag="VGE59_04445"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY1074906.1"
                     /translation="METIYFNGITFVGCHGCTTEEKFRPQRIITDVAWQADVHESFLS
                     DAPLDTVDWMPIQKIVEEVVTGKSRNTVEALASRIAVLVLSNDARTQSVTVSVSKPEE
                     LADRNGTPGVTVTLSREVWEQTWVPKGFTADWASTT"
     gene            6857..7216
                     /locus_tag="VGE59_04450"
     CDS             6857..7216
                     /locus_tag="VGE59_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074907.1"
                     /translation="MSSRNRRTPLMERLTNNLVIRYKIGRVDIDELYRAEEASWLWLA
                     VSEEIRLLSPEQLGRLASDGLSLADSGSHDRTALYHVHLATWLDKRSSGLELLQRIAA
                     TAVIARLVDRFPRFAQK"
     gene            complement(7326..7817)
                     /locus_tag="VGE59_04455"
     CDS             complement(7326..7817)
                     /locus_tag="VGE59_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074908.1"
                     /translation="MEPRETDHIQRMEKIVDEQRREVLDETKALVPAPEDETEEQREI
                     REFITWLDRFATTLSLPESPDMFVIRTTDTTETDEGERIQIRNRYRAALEEAVNAVSD
                     GVVFRQAQLGLLVRCAALKVQHGEYNAAYEDMEEALQMAQQDPEAFDEATIKELDAML
                     SSM"
     gene            complement(7929..8288)
                     /locus_tag="VGE59_04460"
     CDS             complement(7929..8288)
                     /locus_tag="VGE59_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074909.1"
                     /translation="MKSGELRSTFGVGALFGLISWSCCISAIVLAFLGLSAASGFFAM
                     IQMKYHWWLVGVAFVSMDVAIYYFMKHWHGACDIKTIRHNWAPFVLIILFALAAYFVL
                     QAVLPSLVELSGVPMGM"
     gene            complement(8300..9607)
                     /locus_tag="VGE59_04465"
     CDS             complement(8300..9607)
                     /locus_tag="VGE59_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1074910.1"
                     /translation="MQKSISLSTQLPMEKKIVRNMSAATAILSLITLGISFYFLPGVN
                     FNPVSISLFTLSTLAFLIIYVGLPQLYLNETFLMIGDALYIAVVTYTAYRAGEYGILM
                     LFLYFVIIVADALKYPLSEYVFVVLITLQAAFFYIIVGAPFSSEVRIGILTLFTFCTV
                     SIAVFVWYFVYQTVYERSMRYVLERKTTYLKSMNDHLKAVDNMRTSMLRVTSHEFQTP
                     LVAIRNSLELMRSNNMGKLTPKQLNLLKMATTNNERLTTLVKELLNAARIEAGDWKMN
                     LTKVDIRDLIEDVLDEYEPIMKEHKHKLISEFPRHKLFMKADRDLLRISLKNLVDNAA
                     KYTPDGGKIEVKLLESATDVIISVRDSGIGIKKELLSHLFSQFYRTEKAIETKPDGYG
                     IGLYHSQLIVKRHKGKITVISEEGKGSTFEIHLPKSALVRKSV"
     gene            complement(9627..10013)
                     /locus_tag="VGE59_04470"
     CDS             complement(9627..10013)
                     /locus_tag="VGE59_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1074911.1"
                     /translation="MPEKASKTILIIDDDATLRETYQMIFQEKGFTAITAKDGRSGIE
                     AAAENKPDIIILDLMMPGMNGKEVLGELKANADLKKIPVIVSTALIEDLEQGEVKSLG
                     AEAYTVKTQIEPDDLVALAKKVLHTK"
     gene            complement(10097..11575)
                     /locus_tag="VGE59_04475"
     CDS             complement(10097..11575)
                     /locus_tag="VGE59_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY1074912.1"
                     /translation="MSKKEKPKSQPIQKHALFKGKYKGLFLAVLLLVIGNLLLWGNFS
                     LQRIRAGKFPVGTSLHGQSLSGLTKAQAEAKLSPVYESILTHPVTLHIDSHTFQTTPQ
                     ELGLTIDTTTTVSQAFSQLNQRSLLQQVTSIIPFKSHPLTNLAYTINEETLTAYFNQI
                     NKELPHESVDLSFDYQNGSLVITPPITALTFDITQGVDTIKQINPLALPDNITLPVTT
                     QLPVITTEVQAQAAQTLLNKVLAQPLKIEIDEIKTELSPQDIFNTAIFAQEDNKLVVT
                     FDETALKDLVSKIAKQVDRTPITRKVFTHDNSVLAEGRDGRKLNVTQLTKVIAEKLTT
                     GETDTPIVMAADTINRSVIQESPEYELGRTEGKYIDVDLSLQKLNIIEGSTHIRSFSV
                     STGSWSTPTPIGQFTILNHQQTAWSKKFKLYMPFWMAIKAEAGAYDGYGIHGLPYWPS
                     GKVEGTSHIGRPVSHGCIRLGPGDAETVYAWADNGTKVFIHQ"
     gene            complement(11865..12392)
                     /locus_tag="VGE59_04480"
     CDS             complement(11865..12392)
                     /locus_tag="VGE59_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074913.1"
                     /translation="MQYLLITFLIFMLLLAIFYVILGLYFLRDCFFGLPYVGTKKEII
                     DTMLSMAKVKPGEKAIDIGSGDGRLVIALAQAGTEAHGVEINPWLVWYSRWQIARHGL
                     KGKAFIHRKSLWDQDYSDFTLVTVYALPGIMNKLEGKLHKELPPHARIVSNGFTFPTW
                     KEKINNSGTRLYTNT"
     gene            12458..13468
                     /gene="fni"
                     /locus_tag="VGE59_04485"
     CDS             12458..13468
                     /gene="fni"
                     /locus_tag="VGE59_04485"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEY1074914.1"
                     /translation="MSDISERKQAHLDLCLEPNSQGSGNLFSEYKLPYRALPEINLAD
                     VSTETELFKKRLSQPLIIASMTGGVAHAQTINTNLAIAAEACGVAMGVGSQRIALEKD
                     EARETFELVRKHAPTAVIFANMGAIQLNYGRTVDDYRRVVDMIGADALYLHINPLQEA
                     IQPGGDTNYAGLLDKIADVVAKVGVPVFAKEVGHGLDVVTAKALIERGVKGVDCAGTS
                     GTSWAWVEAKRAGDSAFESWFKEYGLRSNTLLEQYRELPQDIYKVVSGGVRTPIEAVK
                     ARALGANFYSFAQPFLAPALEGPEAVAAFIRHFQRGIAITLFTAGCKNWEAAAQLPLQ
                     KK"
BASE COUNT         3349 a         2988 c         3589 g         3609 t
ORIGIN      
        1 tatcctgcaa gggcatgccc gtgcctacgt cacggtgggt cgtcgggttg aaacccggca
       61 attcacgctg ccgttacgca cgccgaggga agcggttaag tctctcttcg ccggtgtccg
      121 tcatgattgc cctggcgcca atcaagttca ggtggtagct ctgactctcg agctgcctac
      181 gggagaaacg ctgcaagatc tcgaagatcc ctccaggtac ctgtctgcat tcccgtattt
      241 ggccacggtg tggcctatct tctgatcggg actccggcgg cgtgtgggaa taagcccggg
      301 gaggtgaccg atcatctcat gtgcggctaa gcatccggcc ttccgcggta agtcttgtag
      361 ctcaggggta gagcgctcga gcttgctcga gaagtcggtg gttcgaatcc actcaagact
      421 gttatttcgc cagcgatccg ctggcgattt tctttataac aaaaacaccc atttggtggg
      481 cgtacatgga ctcgaaccat ggacctcgtc attatcagtg acgcgctcta accagctgag
      541 ctatacgccc atacaaatgg gtattattac tctgttaatt ttacacgata tttatgcttt
      601 catcaagttc gttagtaaaa gtgatgtcct ttaataaaat gcactccgcc ttttcaggcg
      661 gagtgagaga ggtttgcgac taggggctga gttacacgac gacccgtcgc cgcataagtt
      721 ggttggcaag cgtaccagta aagaggattc cggctgctat taccaaactt ggagccagtg
      781 ttccgatgcg gcaagccgca atgatcggca aggtacccca cagagcggcg tcgtgcacga
      841 tcgccatgag ttgtcgtgcg aatcggctcg gaaggtagga tctgtacaag atcgataccg
      901 agttaatcca cacgacgagg agtaatggcg tacttacaaa ggcaggcatg agacccacga
      961 gggcaaccac catacccatg cgtccggctg gagttcgatg tctgaaggat gcatggtact
     1021 ggccgtaaac aacgcggatc tggatgtacg accagatggc gatcgtaacg ccagttgcca
     1081 gagctatatc atcgagcccg atgggctgac cggcagctat cagacatccg aggaagagta
     1141 ctgccccgag cacaggtgct gtaatacacc accagtctat tttctgtgga tatacgaagc
     1201 gaaattgctt gtcttccacc ctgtcggctt tgtcctttcc gctttcgcga atgaagataa
     1261 aagcaaaagc gctcattacc cacatccaga actggggagg gtactgagcg gctagcatga
     1321 atgacgtacc cgctacgcat gttgccacaa cgattctctt aagatagggt actcgcccga
     1381 agagatagct gtaccaatat cccaaataga aaagcatagc caacagaacg ttgttgtact
     1441 ccagttgtcg cagcataact gtaattcctg gaagaagata gagcgccact tcataggctt
     1501 tgactgggtt agtcgcagcc agagtttttt tcgcgggatt aagccgatcg atgtgccggt
     1561 ctattacgtt gttaaacgcg ttgatccatc ctacagagag gatggcgcca attgaaccgt
     1621 agagaactcc ccagccgagc gaccctgcca agcaggctcc cgcgacgaca ggaatccaag
     1681 ccaataacga aattggccga actgtgccaa gcaggaactt catggatttt ctccagggcg
     1741 tcgaacagag aagtacttct ccggaatttt aacaaaatca cacgaggctg tatacctttc
     1801 catctctaga ttaacaagtt attattactt taattagata tatagaggta actcgtatgc
     1861 atacagtcga tttttataaa ggggtagaag gcaagaaaca agccgccttt gttattttat
     1921 atgcgttaga tggtatgccc tgggaagaac atcccaagct tgatcgtcat ggattggttg
     1981 ttccaacatg cttaatgatg aaccggtggg ataactggtg cggatttatt ggaggtaagg
     2041 tagatccagg cgaaacctta gaacaggccg caattcgtga agtagaagag gaaatcggcc
     2101 atacgttaac actcagtctg gaacccatcg tggcccatga ctttggccca cttaccaccc
     2161 atgcctttgc ggcgcaggta acctatcagg agttacgtaa gttgcaggaa caagcatctc
     2221 attcacctca cttcggttca gaagtaacag gtgtgttttt accgcatttg gtggactacg
     2281 aacaaaagaa cattactcaa gatagtagta tggtgagttt attaaaatcc tcattcgctc
     2341 cgacggttcg ggaagagatg gttcacttcc tcctgaaaaa agggatattt cgcaaagaag
     2401 acttagcggg tatctgtgac cgggcgggat ttagcttaga tcagttatta caataaatga
     2461 gatattatcc gtaaaagaag ccgcgcgtac gaatacgcgc ggtctttgtt cgtccgggat
     2521 tgggtaagtc ttactcccat atcacctcgc tcaccgagat catccattga agttcggatt
     2581 tctctcctag tgaaatgagg caaggaagct tgagtggttt taggtggccg tttacgttgc
     2641 cgggtgacgc gtacattccg gatttttcgg caagagttct ccagggatga ggtcggttcc
     2701 gattctcttc ccgaagcatc cagacgagga tagggtgagc acccatccgt aactggcgga
     2761 agcaccggat tgcggcgagc ccgccatgtc ggtcgtaaac tcggatttgt cgaatctcgg
     2821 aatcttttac tccttgcaat acaacccggt aaggctccag gactgccggg tgagattcgt
     2881 cgtcgacgag gggattcaga gcctcaaaaa actgttgctc gattatcccc atattaactg
     2941 ctggattcac ctgttggaca acacggaaag ccaagccgat cttctctcga aggagatcat
     3001 cgagtgccat gtaattgtta ctcccaagag gactatacca agtaaggagg tcgactacaa
     3061 ggagtgactg ttttttgtta tcatagctat atgattacca ttgctcatgt cttagtaggc
     3121 ggtgcggttg gggttgttac cggaaatccg cttgcagcgt tagtagccgg aacggcttct
     3181 cattttgcga tggatcttat tccacactta gatcatcctc cctacgctcc aaaagataaa
     3241 aaaggtgatg ttatcttttc gcaggcgatt tatgttcagg cgttttgtga tgttattatc
     3301 gccgctacaa tcgtgtgtat tctttggtat tcctggtttg atttctcgac gcttcatccg
     3361 tttgcgtggg gtgcctttgg tggatttctt ccagatttaa tcgataacgt gccattttgg
     3421 aataagccat ttcgtaagtc ttggttcggg agccgttttc accgctttca tgacgcgatc
     3481 catcacgtat ggacaaagac attcccgatg caccgctatt ggctcttggg tattctcacc
     3541 caggtggttg cagtcgttat tgcctggaat gtcttgaaaa attacgtata aaaacgtata
     3601 attagacagt actttcaatt gacagcttgt cagttggtta cgaataatct gtgaagacag
     3661 attcagcagt aacttcctta aataagtttg tgagcccttt taagggcctg tagctcagct
     3721 ggtagagcaa ctcctttgca aggagaaggt ccgcggttcg aatccgcgca ggtccaccaa
     3781 cacaaaaccg ggttaacgcc cggttttgtg tttcatggaa gtaattcttt tccgtaagac
     3841 gtaagtgcgg tataattact ccatatgaat aagggccatt agctcagttg gtagagcgca
     3901 gcattcgcat tgctgaggtc aggagttcga ctctcctatg gtccaccatg aaaatgcccc
     3961 ggtttgcgcc gaggtatttt catggttagt tattgtctta gcgttcgacc cctaaggttt
     4021 atataaatcg gtggacatct ttgtggaata gccttgctaa agcaatgttt ttagtatata
     4081 atttacgtgc atcgacggcg gtacaactag aggaggttgc gatggctgag aatctgtacc
     4141 agcttaccaa aagagaaact ggtgagcgcg ttggtttgcc gcgggaacgc ggtattgtag
     4201 ttcgggaggc tatgaggttg atcaacaagc cttccggatt cgacatcatc acctatccgg
     4261 accagtggga ggaagggacg ggtgaagtgc atgtgggtac tcgcgcgccc ggtgaagacg
     4321 ccttgcccgt gggcgagttg gatgcctacg agtacgtgat tcggcctgcg acagcagacg
     4381 acctggctgc tgcgtccgcc gactaacgga cgtgcggctt cgagtatagg tacgaaacag
     4441 ctgtttcgta cctatactca cgaaaacttt tttcgaatat tctttctatt gcatatttgt
     4501 gttatacttt atttatgatt ttgaataaaa gattaacgac ttggacaagt tttatctgga
     4561 ctgcttcctt ctttaccgca cgagaaggca ggttttgagt tacgcgaatt aatcaaaacc
     4621 tggaaacctg cctatctcgg gcaggttttt tgattggaaa agggagcaca tgaacaactc
     4681 aatcgttgct ggatttacac gtcaacaatt ctcttggatt ctgatgtttt ttgaaggagg
     4741 aggcgtgttc accgcggtgc tgtggaatcc acaggccaac caggtcttta tcacaagtag
     4801 tgaaactcct cttagggtag ttactgtttc gacgaacaag gagcactacc ttcgacatct
     4861 atgaagaggc gccgtctcag acggaccgag tggtagagta ccgtggctat gaatggcgag
     4921 tcttgcccgg tgtttttgcg ccataccctg cttttagctc taagaaaggg ttaagcggaa
     4981 aaaacattga catgtatctg ggacggcgag tcttggatct ggggtgtggc tgtggcgtac
     5041 gtgggatcat ggcgggatta tgcggggcga aggaggttat tgcgacggac atttgtgagg
     5101 tggcctgccg gaacacgctg ttgaacattc agcgtcaccg tacgttccgc gttaaggtgg
     5161 tgtgcacgga tatgttttcg gggctccgtg ggtcgttcga cacaatcgtg gcgtacttgc
     5221 catcgcgtga cgctgctgtg agcaagccgt gggaacgggc catctatgac ccgggtttgc
     5281 ggctcaatcg gcaattattt gacgaagctt ggcagtacct cgttccaggt ggcacgcttc
     5341 atgcgtcgct cttggatcaa ggtgcaattc ccgagtttct ggatcaaata gcgagtcgag
     5401 gctacgaact tacgtctcac aaggtttgtc cgcacgaaac cggtgattgg catcccttgt
     5461 ctctgcgctg gccggtagtg gtcacgccaa aggagggtaa ccatggttaa ctcgttgtta
     5521 cgtccgtttc tgccgcttgg accggaagtc gtcgttacgg tgatgcagcg tttaggagcg
     5581 atctgttttc ctatcatgag tgaggcagac gcgctggctc ttgctgtaga agcgaagcgg
     5641 tttaagtaca agaagcaacc taaggagtat gggaagcgca aggttcgtca ggaggtttca
     5701 agctgcttgg tatcggaagg agccataggc gacttcgcca gcagtttaga acgcgagctg
     5761 gcagtgttct ttggtccaga agcttttgag tcgccgcttt cgtttacggc gcggaacatc
     5821 cagcgttacc gtaatggaac tctggggatt gagccacacc gggacgaaag tgagaaccgt
     5881 aatatcgtag ctcttctagg gctagaagga aggggaagat ttactgttta cgacgagctg
     5941 gatgggccgg ttcgagtgcg ctggactatt aatccgggag acctgcttct tatggttgcg
     6001 cccggattct atggagccga tgcgtcttgc cgccgagttc atcgagtaga tgagattgtt
     6061 ggctaccctc accggtatct gcttgggtta cggcagtacg tgccctcagc gtccaggcaa
     6121 ggagaaaagt aatggagaca atctacttta acgggatcac gtttgttgga tgtcatggct
     6181 gtacgacgga agaaaagttc cgaccgcagc gcatcataac cgacgtcgcc tggcaagccg
     6241 acgtccatga gtcgtttctg agtgacgccc ctctcgacac cgtcgactgg atgccaattc
     6301 agaagattgt ggaggaggtc gtcactggta agtcacgcaa caccgtcgaa gctttagcga
     6361 gccggattgc agtgctggtt ttgagcaacg atgcacgtac ccagtcggta acggtatcgg
     6421 tatcaaagcc agaagagctg gcggatcgca acggaacgcc tggtgtcacc gttacgcttt
     6481 cacgggaagt gtgggagcag acgtgggtac cgaagggatt caccgccgac tgggcgagta
     6541 ccacgtagga cccccatgtt cggtatttcg taagccactc gtcagaggaa tctgtacggg
     6601 tggctttttt taatcttgac ataatatact ttttatgcta tgattctgtg tgttcgaaaa
     6661 taacccacgg acgcatgtcc gttttttgcc caacggggta gtgagtaggc catcaggcca
     6721 ttgtccctca gggactttct gccgtaaggc gattagctcc tcaggagcgt ttactcgaaa
     6781 accccggttg gaacccagca attgtgtatg gacctccggc gagctggcga gctcgtaaaa
     6841 acacgctgga ggtgccatga gcagccgaaa tcgacgaacc ccgctgatgg agcgactcac
     6901 caataacctg gtgattcgct acaaaatcgg tcgggtcgac atcgatgagc tgtacagggc
     6961 agaggaggca tcgtggcttt ggttggcggt ctcggaagag attcgtctgc tgagccctga
     7021 gcagctcgga cgcctagcgt ccgacggtct cagcctcgct gactcgggct cgcacgatcg
     7081 cactgcgctc taccacgtcc acctggcgac gtggctagac aagcgatcaa gcggtctcga
     7141 gctgttgcag cggatcgccg caacagccgt aatcgcacgc ctggtcgacc gctttccgcg
     7201 ctttgcgcag aagtagttga tcagaggccg agccatctgg ctcggtttct tgtttttata
     7261 taaaaaagag cgggctgggc cggcggggca cagctcgctc ttgtagtgag tacgcgaggg
     7321 tgggactaca tggaggaaag catggcgtcg agttctttga ttgtggcttc gtcgaaggct
     7381 tcgggatctt gttgcgccat ctggagtgcc tcttccatgt cttcgtaagc ggcgttgtac
     7441 tcgccgtgct ggactttgag ggccgcacag cgaaccagta gacctagttg ggcctgccgg
     7501 aacacgacgc catctgacac cgcgttgacg gcctcttcta aggctgcgcg gtagcggtta
     7561 cggatctgga ttctctctcc ttcgtcggtc tcagtcgtgt ccgttgtgcg aatcacgaac
     7621 atgtcggggg actccggtag agaaagcgtg gtcgcgaacc ggtctaacca ggttatgaac
     7681 tcgcggatct ctctctgttc ttctgtttcg tcctcggggg cagggacgag cgcctttgtc
     7741 tcgtcgagca cttctctcct ttgctcgtca acgatcttct ccattctctg tatatgatct
     7801 gtctcacgtg gctccatagt gcctcctcaa tcaaaacgct tataatatat attattttat
     7861 taaaaaatac aagagtaaac aaaaccaccg ccttgtcagc ggtggttttg tatctcggaa
     7921 gagattgttt acattcccat aggaacgcca gagagttcga ccagtgaagg aagaacagcc
     7981 tggagaacaa agtaggcagc taatgcaaag agaataatga gaacgaatgg tgcccagttg
     8041 tgacgaatgg tcttgatgtc acaggctcca tgccagtgct tcatgaagta gtagatagca
     8101 acgtccatgc ttacaaaggc aacgccgact aaccaccagt gatacttcat ttggatcatg
     8161 gcaaagaatc cagaagcggc gctgaggccg aggaaagcaa gaacaatagc agaaatacaa
     8221 cagctccaag agataaggcc aaaaagggca cctactccaa atgtcgaacg taattcacct
     8281 gatttcataa taactccttt caaacagatt tacgcacaag ggcagatttt ggtaagtgaa
     8341 tctcgaaggt cgaccctttg ccttcttcac taataacagt aatcttgcct ttgtgtcgtt
     8401 taacaataag ttgggaatgg tacagcccaa tcccatagcc gtcaggcttg gtttcgatag
     8461 ctttctcggt tcggtagaac tgagagaata agtgggaaag aagttctttt tttataccaa
     8521 taccactgtc tcggacgctg ataataacat ctgtagcaga ttcgaggagt ttgacctcaa
     8581 tcttgccgcc atcaggggtg tattttgcgg cgttatcaac gaggtttttt aatgagatac
     8641 gtagaagatc tcggtcggct ttcatgaaaa gcttatgacg ggggaattca gatatcagtt
     8701 tgtgcttgtg ctctttcata attggctcat attcatcgag cacgtcttca atgaggtctc
     8761 ggatatcgac tttcgtaagg ttcattttcc agtctcccgc ttcgatacgg gcggcgttta
     8821 ataattcttt gaccagggta gttaatcgtt cattattggt cgtggccatt tttaaaagat
     8881 ttaattgttt tggcgtgagc ttacccatat tattactacg cattaattcg agcgagttgc
     8941 gaatagcgac gaggggagtt tggaactcgt gggaagtaac gcgaagcatg ctcgtacgca
     9001 tgttatcgac agctttcagg tggtcattca tggacttaag ataggtcgtt tttcgttcta
     9061 atacatagcg catggagcgt tcataaacgg tttggtacac aaagtaccag acgaacaccg
     9121 caatagagac ggtacaaaac gtaaagagcg ttaggatgcc aattctcact tccgagctaa
     9181 agggagctcc aacaataata tagaaaaaag cagcttgcaa ggtaatgagg acaacaaaga
     9241 catattccga gagggggtac tttaaggcgt cggctacaat aataacgaag taaaggaaaa
     9301 gcatgaggat gccatattca ccggctcgat aggctgtata ggtaactacc gcaatataaa
     9361 gggcatcgcc gatcattaag aatgtttcgt tgaggtagag ttgcggtaac ccaacataaa
     9421 taatgaggaa tgcgagggtt gagagcgtga aaaggctaat gctgacaggg ttaaaattaa
     9481 caccgggaag aaagtaaaaa gagataccga gagtgatcag tgacaaaata gcagtggccg
     9541 cactcatgtt gcggacaatt tttttctcca tggggagctg tgtgctcagg gaaatgcttt
     9601 tttgcacggt cctctcctta cctggcttac tttgtgtgga gtactttttt ggctagggcg
     9661 actaaatcgt ctggttcaat ttgtgtcttg accgtgtagg cttcggcgcc gaggcttttc
     9721 acttcgcctt gttctagatc ttcaattaag gcagtgctta cgataacagg gatctttttt
     9781 agatcggcat tggctttaag ttctccgaga acttctttgc cattcatacc tggcatcatc
     9841 agatcaagga taataatatc aggcttattt tcggcagcgg cttcgatgcc actgcggcca
     9901 tctttggcag tgatggccgt aaagcctttt tcttgaaaaa tcatttgata ggtttcgcgc
     9961 agggtcgcat cgtcgtcgat aataaggatt gtcttcgaag ctttctctgg catatgccct
    10021 cctgtgattc tggatataca ctcactatag ctgaggtaat ggcgattgtc taaactcttg
    10081 ctaatttcga cgatctttac tgatggataa agacctttgt accattgtct gcccaagcgt
    10141 aaacagtctc tgcgtcgcct ggccccagtc gaatacagcc gtggctcacg ggtcggccaa
    10201 tatggcttgt cccttctact ttaccgctcg gccaataggg aagaccatga atgccatagc
    10261 cgtcgtaagc gcctgcctcg gctttaatgg ccatccagaa cggcatgtag agtttgaatt
    10321 ttttggacca tgccgtttgt tgatggttga gaatagtaaa ctgaccaatg ggtgtagggg
    10381 tgctccagct tccggtagaa acagaaaagc ttcggatatg cgtgcttcct tcaataatgt
    10441 tcaacttttg gagcgataaa tcgacatcaa tatactttcc ttcggttcgg ccaagttcgt
    10501 attcgggact ctcttggatg acactgcggt tgatggtgtc ggctgccata acaattggcg
    10561 tatcggtctc tccggttgtt agtttctctg caatgacttt tgtcagctgt gtgacattca
    10621 attttctgcc atcccgtcct tcggcgagga cactgttgtc gtgggtaaag accttgcgtg
    10681 tgatcggtgt acggtcgacc tgtttggcga ttttagacac aagatctttt agggctgttt
    10741 cgtcaaacgt cacaacaagt ttattgtcct cctgagcaaa aatagccgtg ttaaaaatat
    10801 cttgaggtga tagctcggtt tttatttcat cgatctcgat ctttaagggc tgggcgagca
    10861 ctttgttaag taacgtttga gctgcttgag cttgcacctc agtggtaatc acgggcaact
    10921 gggtggtaac aggtaaggtt atgttgtcgg gaagtgccag cggattaatt tgtttgatag
    10981 tgtctacgcc ttgggtgata tcaaaggtga gagccgtaat aggaggtgtg atcacgagtg
    11041 aaccattttg gtagtcaaaa gagaggtcga cactctcatg gggtagctct ttgttaatct
    11101 gattaaagta cgcagtgagg gtttcttcgt taatcgtgta agcaagattg gttaggggat
    11161 gagacttgaa aggaatgatc gaggtaacct gttggagtaa agagcgctga ttcagttgtg
    11221 aaaaagcttg tgacacggtg gttgttgtgt cgatcgttaa acctaattcc tggggagttg
    11281 tctggaacgt gtgggagtcg atatgaagcg ttacggggtg tgtaaggatt gattcataaa
    11341 ccggggatag ctttgcttct gcttgggctt tggtgaggcc actgagtgat tggccatgca
    11401 gcgaagttcc aaccgggaac tttccagctc gtatacgttg aaggctaaaa tttccccata
    11461 aaaggagatt gccaatcacg agaagaagaa ctgcaagaaa cagtcccttg tatttccctt
    11521 taaagagagc atgtttttgg attggttgtg atttaggttt ttctttttta gacatgggag
    11581 attttaccca cgcattggaa agcggccatg tatgtattat atagaaacat cctaattcct
    11641 aaagggggag aaatgtataa gccccagatc acggcaggtg atccggggct tatggtgctc
    11701 ttttagcgaa agagctggac gaggaaggga gataccaaaa cagctaacgc gaacgaaatg
    11761 gcaagtccga caagggcgga aacttcgttg ttcaacgtca cgatctccat acagacagag
    11821 cgatggagtt atttaatcac gaaaatatag tcgggtcaag tgtcctaggt gttagtatag
    11881 agacgagtac ccgaattgtt tatcttttct ttccaggtag gaaaagtaaa tccgttggag
    11941 acaatgcggg catgcggagg gagttctttg tgaagtttgc cttcaagttt gttcataatt
    12001 cctggtaagg cgtacacggt gacaagggta aagtcgctat aatcttgatc ccacaggctt
    12061 ttgcggtgaa taaaggcttt gccttttaat ccatgtcggg cgatttgcca acgtgagtac
    12121 cagacgagcc atggattgat ttcgacgccg tgggcttctg ttccagcttg cgccagggca
    12181 atcacaaggc gcccatcacc agaaccgata tcaatggctt tttctcctgg tttaactttt
    12241 gccatactga gcatcgtatc tataatttct ttttttgtac ctacatacgg taagccgaag
    12301 aagcaatctc gtaaaaaata gagacccaaa ataacgtaaa agatggctaa gagaagcata
    12361 aagattaaaa atgtaattaa aaggtactgc atgttttgta tcctagcagc gaaaaaaagg
    12421 aatggcaatt ttgcgagaag cgagctaaaa tacgcctatg tctgatatct ccgagcgtaa
    12481 acaagcccat ctcgaccttt gtcttgaacc taattcccaa ggctccggaa atcttttctc
    12541 ggagtacaaa ttgccatatc gggcattgcc ggagataaat cttgccgatg taagtaccga
    12601 aaccgagctt tttaaaaaga gattaagcca accgcttatt attgcttcaa tgacgggtgg
    12661 cgtggcgcat gcgcaaacga ttaatacgaa tttagcaatt gcggccgaag cgtgtggggt
    12721 agcgatgggg gttggttcgc aacggattgc attagaaaaa gatgaagctc gtgagacctt
    12781 tgagctggtt cgtaaacacg ctcctaccgc cgtcatcttt gccaacatgg gggcaattca
    12841 actcaattat ggccggacag tcgatgacta tcgtcgggtc gtggatatga ttggtgccga
    12901 tgcactctat ttacatataa acccacttca agaggcaatt cagcccggtg gcgatacgaa
    12961 ctacgcagga cttctggata agatcgcgga tgttgtggct aaggtaggtg tgcctgtctt
    13021 cgcaaaagaa gtcgggcatg ggcttgatgt ggtaacggcc aaagcattga ttgagcgagg
    13081 tgtgaaaggg gtggattgtg ccggtacgag cggtacgtcg tgggcttggg tggaagcaaa
    13141 acgggccgga gattcggctt ttgaatcttg gtttaaagaa tatggccttc gtagtaatac
    13201 cttactagag cagtatcgag agttgccaca agatatatat aaagtagtga gcggaggagt
    13261 tcgtactcca atcgaagcag tcaaagctcg ggcgttaggt gctaactttt actcctttgc
    13321 ccaaccattt ttagcgcctg ctctggaagg tcctgaagcg gtcgctgcat ttattcgcca
    13381 ttttcagcgt gggattgcga ttactctgtt tactgcaggc tgtaagaatt gggaggccgc
    13441 agctcagctt cctcttcaaa aaaaataggc gtgtgttaga gtagtattat gttaggtctc
    13501 tcaatctgga tgaaactatt ttggttttta gtgtg
//
LOCUS       DASWYB010000076         6111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_910520, whole genome shotgun sequence.
ACCESSION   DASWYB010000076 DASWYB010000000
VERSION     DASWYB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910520"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="VGE59_04490"
     CDS             <1..1508
                     /locus_tag="VGE59_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074915.1"
                     /translation="EIKEAGNRLYEMFRTMLGGSSSKRQLADGKTFEESSSSSAIERY
                     TRKLGDSVVVGLDKLLETQFEFITKGELNENGKFNANGTPYPPGLIAKIKAGYFSNKE
                     ARNTFIFGMIDARIRAVDPSLPQDFTKVLVEGSNKDRSLVLYNYLHARLEGQVLASVP
                     EDLRAPVSLFLQSYNPKTGSFALNSGEDFIANPAFMGWASATISKYTGNRIPMDAMRL
                     GIKYLTDKFKPQVLAPNSTTAKAFDPSYLDGGLDNKLKSEFQKETGGTYLSNPLAETT
                     DPSIVNAAEGEVTNPTTKEKGKFDYKGALKAMAVDYMIEFVGKRSSFPVGQMKAFYDK
                     YKAAQGLYKDFKAGKVDVAQAVFIVDDLFFGGKISALLTGFDDALGLPAGSTQALLMF
                     AITGNPMYAVQFVLGFIFGSTMECPDYQLEARKNVKKLIRKVIKLGEFDASLIPSQII
                     TFSQTYLDQLSGVIDRNYHDYGGLGYDPCPPLSRCGVFARPEYGFQVHIGF"
     gene            1591..2604
                     /locus_tag="VGE59_04495"
     CDS             1591..2604
                     /locus_tag="VGE59_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY1074916.1"
                     /translation="MNVHLRTQFANPVLNRDFPDPAILPRRVNGKYYAFATQESPHNP
                     TIQMATSEDLITWELSPRGAFARKPIFAQHSRDYWAPHIIEDIEYNRFIMAYTVRSDS
                     TGQLGISLAIASDLDEGFIDPHTQPLLEGPGYSIIDPHYLIDPRTGKRWLYWGSHETP
                     IMMQELDATGLRFAVGSVPQAVLHPIPEHPVLRLLEGFFVFYHPGCNMFFGLASGPNT
                     WEPDGYHVSCFWAKDPTGPFEPIPHDMVIVSPSQRWLAPGQCSIFCDEGNQYWLYLHA
                     VDVQNPHNNFSNKQIARVMCAVPLDFSQGYPQTVGREVPCQAIAGPSIQTLDTQTVQI
                     GMY"
     gene            2785..3444
                     /locus_tag="VGE59_04500"
     CDS             2785..3444
                     /locus_tag="VGE59_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY1074917.1"
                     /translation="MTSPLRKPSFIINEASLDLVSDLGITHRDTFEQSLFVEVKANSL
                     VSPALKIEHSIIRKSDLSLSELKKIQLTEVIIEDTDISNSQWFEGSLGKVIFKKCRMT
                     GIQLNEAFLEDVIFIECILDLAQFRFSHFKNVVFENCILRETDFQNSNLKGTIFKTCD
                     LGKSRLVNVNLQDADLRGSQLTSLIVTMNDFKGAIINSEQLMTITYDIAEGLGLQIKD
                     E"
     gene            3527..4033
                     /locus_tag="VGE59_04505"
     CDS             3527..4033
                     /locus_tag="VGE59_04505"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY1074918.1"
                     /translation="MKLHSTSIQTPLGRLTFIIQKKGEDVVVAAGFSEREELRQRLPK
                     ELQSYDVEEISHHPYKDAVIAYFSGNLAALSTIKHEQPGSPFSKKIWDVMQTIPAGKT
                     ITYKDLAQKSGSPAAIRAAGTVCAQNRLVLLVPCHRIVPSGGKIGNYLYGTSLKVSLL
                     KHEAQFSS"
     gene            complement(4023..4367)
                     /locus_tag="VGE59_04510"
     CDS             complement(4023..4367)
                     /locus_tag="VGE59_04510"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY1074919.1"
                     /translation="MSIIDTYLEKVSEPEKIALERVRKIIKKTVPDAEESISYGMPAF
                     KYKKKYLVAFCEFKDHMSLFPGSEAIEKFKAKLEGYKLSKGTIQFTLEHPLSETLIRE
                     IVLSRVDDITKS"
     gene            complement(4387..4839)
                     /locus_tag="VGE59_04515"
     CDS             complement(4387..4839)
                     /locus_tag="VGE59_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074920.1"
                     /translation="MKVILLWLINEKGELLLSQRAAHRKTDASAWGPSVSGKVDSQED
                     LKSAVIRETYEELGLELKAELLTYLGEITYSNHADGREREFSMYSASVTSNIIPELRL
                     NLDEVAEVKWIEVNELRKLQKAGAQEITNVHSHELWKNIFSNLEVINV"
     gene            complement(4898..5173)
                     /locus_tag="VGE59_04520"
     CDS             complement(4898..5173)
                     /locus_tag="VGE59_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074921.1"
                     /translation="MDSLHDAMMKRMERVGLEKALSATFIIEAANRVLPQQFRAKTFR
                     NGVLMLEANVAAHAYIYKQEEETFLEQINAALPEHVIEKLRLRVVPK"
     gene            complement(5175..>6111)
                     /gene="recF"
                     /locus_tag="VGE59_04525"
     CDS             complement(5175..>6111)
                     /gene="recF"
                     /locus_tag="VGE59_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEY1074922.1"
                     /translation="VTKSYRVTNDRDAIQWEEKYCRVILERTDDQFEYVLSQEGEAWR
                     KVIKHNGSAIPLTQVYGLLPTILFSPETMQLIDGSPSERRRFLDTVLSQADRAYIEAL
                     VTYRRVIKERHFVLLRLQQGLGSEDELDFWDTQLVQSGSYILEKRQEFLKEINRYLET
                     IYPQFVEEGAREKLVLQYKPTVQISEYADKLRRMRHYDSKRSTTHIGPHRDELTFLLR
                     ERDITLFASRGELRRSVLATKLAETLFLKDKNGHEPVLLLDDVFSELDADRRGHFLEA
                     IKDYTTIITTTDESFVTNSPLPDMLIHRLPLHTKE"
BASE COUNT         1762 a         1410 c         1255 g         1684 t
ORIGIN      
        1 cagagattaa agaggctgga aaccgtctct atgaaatgtt ccgtaccatg cttggcggtt
       61 cgagtagtaa acgccaacta gctgatggaa aaacatttga agagtcttca agcagcagcg
      121 ccatcgaacg ttatacgaga aaattgggtg atagcgttgt ggttggatta gacaaactac
      181 tagagacaca atttgaattc attaccaagg gagaattaaa cgaaaacggt aaatttaacg
      241 ccaacggcac cccctatcct ccaggattaa ttgccaagat taaggctggt tacttctcga
      301 ataaagaagc acgtaatact tttatcttcg gcatgatcga tgctcgtatc cgcgcggtag
      361 atccaagctt accgcaggat tttaccaaag ttctcgtaga gggtagcaac aaagaccggt
      421 ctttggttct ctataactac ctccatgccc gactagaagg ccaagtcttg gcttcggtac
      481 cggaagatct ccgagcacct gtcagcttgt tcctgcaaag ctacaatcct aaaacaggaa
      541 gtttcgccct gaattcaggt gaagatttta ttgctaatcc tgcatttatg ggatgggcca
      601 gcgctacgat tagtaaatac acgggaaacc gtatccccat ggatgccatg cgacttggaa
      661 ttaaatattt aacagataag tttaaacccc aagtacttgc cccgaattca accaccgcca
      721 aagcattcga tccaagttat cttgatggtg gcctcgacaa caaacttaag agtgagtttc
      781 aaaaggaaac cgggggaaca tacttatcta atcccctggc tgaaacaacc gacccgtcaa
      841 tcgtcaatgc ggctgaagga gaagtaacaa acccaacaac taaagaaaaa ggtaaatttg
      901 actataaggg cgcccttaaa gccatggcgg ttgactatat gattgaattt gtaggcaaac
      961 gcagtagctt ccccgtaggc caaatgaagg ccttctacga caaatacaaa gctgcccaag
     1021 gcctctacaa agattttaaa gccggtaagg ttgatgtcgc acaagctgtt ttcattgttg
     1081 atgacctttt ctttggaggg aaaatcagcg ccctgttaac cgggttcgac gacgcactcg
     1141 gcttacctgc tggatcaaca caagcactgc tcatgtttgc aattaccggg aacccgatgt
     1201 atgccgtcca gtttgtctta gggttcatct ttggttcaac catggagtgt ccagactatc
     1261 aactcgaggc tcgaaaaaat gtgaaaaaat taattcgaaa agtgattaaa ctcggagagt
     1321 ttgacgcatc gctcatccca agccaaatca ttacgttcag ccaaacgtac cttgatcagt
     1381 taagtggtgt gattgatcgc aattaccacg actacggcgg cctgggatat gacccctgcc
     1441 ctcctctctc tcgatgtggc gtctttgctc ggcctgagta tggtttccag gtgcatatag
     1501 gattttagca gggtaatagt cccccccttt ctaaaatcat tgtgaatgtg tatagtgcag
     1561 cggttagtcc agaaggagga ataacgctac atgaatgtgc acctacgtac tcagtttgcc
     1621 aatccagtat tgaaccgtga ttttcctgat cccgcaatac tcccacgacg tgttaacgga
     1681 aaatattacg ctttcgcaac ccaagaatca cctcataatc caaccattca aatggcgacg
     1741 tcagaggatt tgataacgtg ggaattatcc ccacgaggcg cttttgcacg taagccaatc
     1801 tttgcgcagc actcgcgaga ttattgggca ccccacatca ttgaggacat agaatataac
     1861 cggttcatta tggcttacac ggtacgatca gatagtactg ggcagctagg gatatcatta
     1921 gcgatcgcga gtgatctaga cgaagggttt atagaccccc acacccagcc acttcttgaa
     1981 ggaccaggtt actcaatcat cgatcctcac tacctcattg atccacgtac cggaaaacgg
     2041 tggctctatt ggggctcaca cgaaacacca atcatgatgc aagagctgga cgcaacaggg
     2101 ctaagatttg cggtcggaag tgtaccgcaa gctgttcttc acccgatccc cgaacatccc
     2161 gtactccgat tgctcgaagg atttttcgtc ttctatcacc ccggttgcaa tatgttcttt
     2221 ggcctcgctt caggaccaaa tacatgggaa ccagatggct atcatgtctc atgtttctgg
     2281 gcaaaagatc ccacaggccc ctttgaaccc ataccgcatg atatggttat cgtcagccct
     2341 tctcaacggt ggttagcacc gggccaatgt tccatctttt gtgacgaagg gaaccaatac
     2401 tggctttacc ttcatgctgt cgatgttcaa aacccgcaca ataacttctc taataaacag
     2461 atagctcggg taatgtgcgc agtaccgctc gacttttccc aaggatatcc ccaaaccgta
     2521 ggtcgagaag ttccctgcca agcaatagcg ggaccaagta tacaaacctt ggacactcaa
     2581 acagtgcaga ttggcatgta ctaaaaatcc cctttcattt tctctccaca gttctttaag
     2641 gggactgaga agaatgccaa tactttcggt ttgtgcttga actatcggat gggcctttac
     2701 gttctttatc ccgcaatcaa ttttgacaga gaggtaacag ggacagagat tggttagtcg
     2761 agataagccc tgtatagtca aaacatgact tcaccactgc gaaagccttc gtttattatt
     2821 aatgaagcat cactcgatct ggtttctgac ttagggataa ctcaccgtga caccttcgaa
     2881 cagtcgctct ttgtcgaagt aaaagcaaat agcctagtct cccccgcgct caaaattgaa
     2941 cactctatta tccgaaaatc agatctaagt ttaagcgaac ttaaaaaaat ccagctgacc
     3001 gaagtcatta tcgaagatac cgatatttct aacagccaat ggtttgaagg gtctctcgga
     3061 aaagttattt ttaaaaagtg tcgcatgacg ggaatccagc taaacgaagc ttttttagaa
     3121 gacgtcatat ttattgagtg tattctcgat ttggctcaat ttcgtttttc acacttcaaa
     3181 aacgtcgtgt tcgaaaattg tattctccga gaaaccgatt tccaaaatag caacctaaaa
     3241 ggaaccattt ttaaaacgtg tgacctagga aaatctcggc tagtgaacgt taatcttcaa
     3301 gatgctgatt tacgtggttc tcaacttacc agcctcattg tcacaatgaa tgactttaaa
     3361 ggtgctatca ttaactctga acaattaatg accatcactt acgatatcgc tgaagggctc
     3421 gggctacaaa taaaagacga ataattttta gtaattcaca actagaattc ggacttcatt
     3481 cgtgagatta tcggctatat cacactctcg aaggaacgct tactatatga aactccactc
     3541 gacctccatc caaacgcctc tcggaagact aactttcatt atccaaaaga aaggggagga
     3601 tgtggtagtc gctgcgggct tcagtgaaag agaagaactc cgccaacgac tccctaaaga
     3661 attacaatct tacgatgtgg aagagatttc tcatcatccg tacaaagatg ctgttatcgc
     3721 ctacttttcg ggcaaccttg ccgcactgtc gacaataaaa catgagcagc caggcagccc
     3781 tttttctaaa aaaatttggg atgtgatgca aactattcca gcgggaaaaa caattactta
     3841 caaggatctc gcacaaaaat caggtagtcc ggctgctatt cgggctgctg gaacagtgtg
     3901 tgcacaaaat cggctcgtac tcctcgtacc atgccaccgg attgttccta gcggcggtaa
     3961 aataggaaat tatttatacg ggacgagcct taaagtttct cttttaaaac acgaagccca
     4021 atttagctct tagtaatatc atcgacacga ctgagcacaa tttctctaat tagtgtttca
     4081 gagagaggat gctcgagcgt aaattggatg gttcctttag ataacttgta tccttcaagt
     4141 ttagctttaa acttctctat cgcttcagag ccggggaaaa ggctcatatg atccttaaac
     4201 tcacaaaaag caaccaaata cttcttttta tacttaaagg ctggcatgcc ataactaatc
     4261 gattcttcgg catccggaac cgttttctta ataattttcc gcacccgctc aagcgcaatt
     4321 ttctcgggct cagaaacttt ttctaagtaa gtatcaataa tagacatatg ttaagaatgg
     4381 tttttgttag acattaatta cttctagatt cgaaaaaata tttttccata attcatgcga
     4441 atgcacgttg gtaatttctt gagcacctgc cttctgaagt tttcgaagct cgttcacctc
     4501 aatccatttt acctcagcta cttcgtctaa gttaagccga agctcaggaa taatgttact
     4561 cgtcacagaa gctgaataca tactaaactc tcgttctctc ccatcggcat gattactgta
     4621 agtaatctca cccaaatagg ttagtaattc agcctttagt tcaagcccca attcctcata
     4681 tgtttcgcga attacggctg atttcagatc ttcttgggaa tctactttac ctgaaacact
     4741 tggtccccaa gcgctagcat ctgtcttccg gtgagcagcg cgttgagaca gtaagagttc
     4801 gcctttttcg ttgatgagcc aaagtaaaat aactttcata cggccatact atcagcttat
     4861 gctcacaccg ttaagcgctt gttataggca ataataatta ctttggtact acacgtaaac
     4921 gtagtttttc tatcacgtgt tcgggaaggg cggcattgat ttgttctaaa aatgtctctt
     4981 cttcttgttt gtaaatgtag gcgtgagcag cgacattcgc ttctaacatg agaacgccat
     5041 tacgaaacgt tttggcgcga aattgttgtg gtaatacgcg gttagccgct tcgataataa
     5101 acgtggcaga aagagccttt tctaagccca cacgctccat tcgtttcatc atggcatcgt
     5161 gaagtgaatc catatcattc ctttgtgtga agtggtaagc gatgaatcag catgtcggga
     5221 agtggagagt tggtaacaaa tgattcatca gtcgtcgtta tgattgttgt atagtctttg
     5281 atggcttcaa gaaaatgccc ccgacggtcg gcatcaagct cagaaaaaac atcgtcgaga
     5341 agaagaacag gttcgtgtcc atttttatct tttaagaaaa gtgtttctgc gagctttgtt
     5401 gccaagacac tccgtcgtaa ctccccgcga gaagcaaaga gagtaatatc tcgttctcgt
     5461 agtagaaaag taagttcatc acgatgaggg ccaatatgcg tcgtcgatcg tttactatca
     5521 tagtgacgca ttcgacgaag cttgtcagca tactcggaga tttgtacagt tggcttatac
     5581 tgtaatacca gtttttcgcg agcaccttct tccacaaact ggggataaat agtctcaaga
     5641 taacggttta tttctttcaa gaattcctgc cgtttttcga gaatatagct ccccgattgc
     5701 actaattgtg tatcccaaaa atccaactca tcttcgctac ccaaaccttg ttgtaaacgt
     5761 aagagaacaa aatggcgctc cttaataaca cgccgatagg taaccaatgc ttcaatatac
     5821 gcccgatctg cctggcttaa aaccgtatct aaaaatctgc gacgttcgga aggagatcca
     5881 tcaatcagtt gcattgtttc tggagaaaac aaaatcgttg gtaagagacc atagacctgg
     5941 gttaaaggaa ttgcgctccc attatgctta atgactttac gccaagcctc tccttcttgg
     6001 cttaagacgt attcaaattg atcatctgtg cgctccaaaa taactcggca gtatttttct
     6061 tcccattgaa ttgcatctcg gtcatttgta acacggtacg atttcgtgac a
//
LOCUS       DASWYB010000077         7942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_247781, whole genome shotgun sequence.
ACCESSION   DASWYB010000077 DASWYB010000000
VERSION     DASWYB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247781"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VGE59_04530"
     CDS             <1..380
                     /locus_tag="VGE59_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074923.1"
                     /translation="RELLNGKVEYFDLVDMYGIMSAALDGSTLWLGFGHSSESHRDVL
                     GETLRVDQSSGFSSLSWKGGGFIGLSLWGQVENGKVPVSVYGISEGLGELHPQVVVAA
                     VEEYCKLLAHYGFEQAVVAPPRE"
     gene            complement(445..1497)
                     /locus_tag="VGE59_04535"
     CDS             complement(445..1497)
                     /locus_tag="VGE59_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEY1074924.1"
                     /translation="MSATQDRTEDSKAVATQAGLRYVSDKSPGFTRKKAGSGFMYLDA
                     KGKHITDPEVIARIQALSIPPAYSDVWICPYENGHIQATGMDARGRKQYRYHTDWQNA
                     RQATKYNRMREFGHALPKIRRQVNADLKLPGVPREKVIATLVQLLEKTLIRVGNEEYA
                     KENKSFGLTTLQSKHVEVEAHEIKFAFKGKSNQYHEITLQDKRLARIVSKLQDLPGQE
                     LFQYVDDNDNLHEIHSDDINQYLQEVTGQDFTAKDFRTWKGTVLAAKALLKCEACKSK
                     TQAKRNLNAAIRDVASELGNTPGVCRKAYIHPKLIATYTTTMTLECLNCTPTQLEEFK
                     HLHKDEASIMVFLENH"
     gene            complement(1527..1967)
                     /locus_tag="VGE59_04540"
     CDS             complement(1527..1967)
                     /locus_tag="VGE59_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074925.1"
                     /translation="MQPLSDKQTIIDQITTLNFPAGQYIILGEGSLAIRGLRDTKDLD
                     ILVEPELFATLAKTWPLDSAYEQKWNRKRLKKGNIEVYPDMYLEKEACFLDVSELIQS
                     AEIVEGIPLQPIDNLLRCKLDTGGEKDLRDVVLIKKYLLNNKTC"
     gene            2021..4264
                     /locus_tag="VGE59_04545"
     CDS             2021..4264
                     /locus_tag="VGE59_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY1074926.1"
                     /translation="MKKILFWIVATLLVIYGVLAIPVPRTPDQSVTWGLTFSQKMSEE
                     LGDDWRAAYEDIVLNLKPQRLRLVAYWDKIESQSGRYVWDDFDWQVKLAKENNIPVTI
                     AIGKKAPRWPECHVPSWADSLASTDQQSAINSYLTAVISRYKAEPNIEYWQVENEPFI
                     GFGACPTLSQSVLEQEVALVRQLDPTHKILLTDGGAWGKWIEPAKLADAFGTTLYTKV
                     YRKGIGYTRPPFPAPYYSKKADIVRAATGQKDQPYIFTEIGLEPWGGKSIAEMSLEEQ
                     RSLHSLKDFRGTLEFARSTGFGTFYLWGGEWWHSLKFKGDDSYWREAQKVFASGRSNN
                     PLADLPDSFLWGASTASHQVEGGTQNDWTEWEKVHANTLAAGATEKFSHQIPNWNAFA
                     SAATSPTNYISSDAADHYHRYAEDFSLAQSLGMNAQRISLEWARIEPKRDVFDAKELD
                     HYRAVIADMKAKGMEPMVTLWHYTLPLWFRDAGGWENPESAKWFAEYSQKVVSELGGD
                     VTFWITLNEPGVYSNNAYLLGTRPPQKQNPVAYLRVHKNLVQAHIQAYAAIKSVAANA
                     QVGIAQDVYSYESNTGLVQQLIRPLAEQWTNYSFLDRLGTSKDFIGLNYYINVTLGGK
                     SSRTGTSDLGWSLYPEGIYSVLKGMARYQVPLYVTESGLADASDTQRADYMASVIAQI
                     AAARKDGVDVRGYFYWSLIDNFEWEKGFWPRFGLIAIDYTTQARMPRPSAFKYADLIA
                     AYRATHQ"
     gene            4308..4733
                     /locus_tag="VGE59_04550"
     CDS             4308..4733
                     /locus_tag="VGE59_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HEY1074927.1"
                     /translation="MKDRIFLYGAWCVAIIAMLGSLFFSEVMHLPPCILCWYQRICMY
                     PLVLILGVGIYTRDKHIVSYVLPLSILGMGIALYHNLLYYGFIPESLAPCRAGISCTT
                     KLIEWWGFVTIPFLSFVAFGLITTAMFLVLKQSKGNNHA"
     gene            4726..5370
                     /locus_tag="VGE59_04555"
     CDS             4726..5370
                     /locus_tag="VGE59_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY1074928.1"
                     /translation="MHDGKIIAGVIAVTVLILGGIFAFGGSSAPKPADPLVVASPELI
                     REHTATVGGGEKVTLVEFGDFQCPACGSAYPVLKQIKADYGDRITFAFRNYPLTIHAN
                     AELAAYAAQAAAEQGKFWEMHDKLFETQSAWSLSFNPENTFVGYATGLGLDEAKFRAD
                     IKNQAVIDKVRLDKGDGEALKVNSTPTLFLNGAQIPSSWSYESLKSLIDQELAK"
     gene            5367..5567
                     /locus_tag="VGE59_04560"
     CDS             5367..5567
                     /locus_tag="VGE59_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074929.1"
                     /translation="MNHIFIAPAFAHEGEVHTESSTAPEPEVQYVSFSPTATVVTYIL
                     LGLIVIVGVLGLIFTYNQRGKQ"
     gene            complement(5628..6455)
                     /locus_tag="VGE59_04565"
     CDS             complement(5628..6455)
                     /locus_tag="VGE59_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074930.1"
                     /translation="MSRVLWIAVIALLSCWTAPAKAEEPVTFHGFWMLTADSSSNKLS
                     DHTRWIVSADLNKDLTATVSVTPRGPLRTFSYGELQWKKPIKGIDQIAVGRSFPPFGR
                     IWYDYRVDQVPVIYYSDIYVPLAATDTGVTVTGSTGRTKWYAGTYVGDPEFGNELGAS
                     SVIRAEYRVTSALTLATSQRISQTPAHGADLLYKSGRMTAIGELVSSDGVTQYLALGE
                     YRVTKHTYLSLQHEWLEAGDRWTVAPRWDITKNATIKVGYQTGANTSRLIGQVGFHW"
     gene            complement(6579..7472)
                     /locus_tag="VGE59_04570"
     CDS             complement(6579..7472)
                     /locus_tag="VGE59_04570"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY1074931.1"
                     /translation="MDSPTMFDTNVAFAGITLHVDPINRDRTDTADNVMAMFHEEQPL
                     TWEVIHPSLEDALKRSRGDIGFLDVGTGSGVFAILVKKNYGNVPVLAIDKSDRAIAFA
                     KSNALANDVAIGFRKETYGYQTARPGSCRAIGIYPPYHLYPNDEDIALKIPQHARGGS
                     DGQREFISQLHYASLHLSKEGILFFNQMCWGDDEGPDFLRYIPRIFPTASFTWCNVFP
                     RISTREFLEGVYAGRTEPTYRRYIEESTQLHPWLYYTVGIITNDDKGEIKEASHQVDL
                     KGRSWKDRIVLHREIAAHAWK"
     gene            7580..>7942
                     /locus_tag="VGE59_04575"
     CDS             7580..>7942
                     /locus_tag="VGE59_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1074932.1"
                     /translation="MYTDTLIQLGLAQNEARIYETLLREGESSVGDIATKSGVHRRNV
                     YDSLNRLLEKGLVFEILERRENRYQAVDPGKLSEMLQEKQRALAGIMPSLESLYSGTP
                     HRNDVFIYKGVECWKNYIR"
BASE COUNT         1816 a         1797 c         2078 g         2251 t
ORIGIN      
        1 ttcgtgaact tcttaacggt aaagttgagt acttcgattt ggtagacatg tatggcatta
       61 tgtcagccgc gttagatgga agcactcttt ggctgggatt tggtcattcc agtgaatccc
      121 atcgtgatgt tcttggagaa acgctgcgtg tcgatcaaag ctcgggcttc tcttccctga
      181 gttggaaagg aggtggtttt attgggcttt cgttatgggg ccaagttgaa aacggaaaag
      241 tgcctgtgag tgtgtacggt atttcggagg gactaggaga gcttcatccc caggtagtgg
      301 tagcagcagt ggaagaatat tgtaaattac tggctcacta cggatttgag caggcggttg
      361 ttgctcctcc tcgcgaatga tccaccattc gcttgtgatt attcttatct ctagacccgt
      421 cagcatgatg ctgacgggtt ttctttaatg attttctaaa aataccatga tgctcgcttc
      481 gtctttgtga aggtgtttga attcttctaa ttgagttggc gtgcagttga ggcactcgag
      541 tgtcattgtt gtggtatacg tggcaatgag cttggggtga atataggctt tgcgacagac
      601 gccaggggtg ttgccaagtt ctgaagccac gtctcggatg gcagcgttta aattgcgttt
      661 tgcttgggtt ttacttttgc aggcttcgca tttaaggagc gcttttgccg ccagaacggt
      721 ccccttccag gtgcgaaagt ctttggcggt aaagtcttgg cctgtaacct cttgtaagta
      781 ctggttgata tcgtcggagt gaatttcgtg taaattgtcg ttgtcgtcaa catattgaaa
      841 aagctcttgg ccgggtaggt cctgaagctt actgacgatg cgagcgagcc ttttatcttg
      901 tagggtaatt tcatgatatt gattactttt ccctttaaag gcaaacttta tttcatgtgc
      961 ttctacctcg acgtgtttac tttgtagtgt ggtgaggcca aatgatttgt tctcttttgc
     1021 gtactcttcg ttcccgactc gaataagtgt tttctccagg agctgaacga gagtggcaat
     1081 aactttttca cgaggaacac cgggtagctt aagatctgca ttgacttgcc ggcgaatttt
     1141 aggcagggca tgtccaaatt ctcgcattcg gttatatttt gttgcctgcc gcgcgttttg
     1201 ccaatctgtg tgataccggt actgcttgcg gccgcgcgca tccatgccgg tggcttgaat
     1261 atggccattt tcgtaggggc agatccagac atcgctataa gcaggaggaa tactaagggc
     1321 ctgaatgcga gcgatgactt ccgggtcagt aatgtgttta cctttggcat caaggtacat
     1381 aaaaccgctg cctgcttttt ttcgggtgaa cccgggtgac ttgtcggaga catagcgaag
     1441 tccagcttgt gtggcgacag cttttgaatc ttcagtgcga tcctgcgtag cgctcatagc
     1501 gtaactcctt gtgacgtaat tttattctag caggttttgt tattgagcaa gtactttttt
     1561 atgagtacca catcgcgcag gtctttttct cctcctgtat cgagtttaca ccgaagtaaa
     1621 ttatcaattg gctgcagtgg aatgccttct acgatttctg cgctctggat tagttctgag
     1681 acgtcgagaa aacaggcttc tttttctaaa tacatatcgg gatagacctc tatgttacct
     1741 tttttaagtc gtttgcgatt ccatttttgc tcatatgctg aatcgagtgg ccacgttttt
     1801 gctaatgtgg caaagagctc aggttctaca aggatatcga ggtcttttgt atctcgaaga
     1861 ccacggattg caagcgaacc ttctccaagg ataatgtact gaccggcggg aaagtttagt
     1921 gtcgttatct gatctatgat ggtctgtttg tcggagagag gttgcatacg aatattgttt
     1981 tctttgaaaa tagcgtactt ccccgtatga tacaaacagt atgaaaaaga ttcttttctg
     2041 gatagttgcc acattgcttg taatctatgg tgtgctggcg attcccgtcc cacgaactcc
     2101 tgatcaatca gttacctggg gcttaacatt ttcacaaaaa atgagcgaag agctaggaga
     2161 tgattggcgt gcggcgtacg aagatattgt ccttaattta aagccacaac gtctccggtt
     2221 ggttgcctac tgggataaga ttgagtcaca gtctggacgc tacgtatggg acgattttga
     2281 ctggcaagtg aagctcgcca aagaaaataa tatccctgtc accattgcga tcggcaaaaa
     2341 agccccacgg tggccggaat gccatgtccc ctcttgggct gactcgctcg catcgacaga
     2401 tcagcagtcg gcaattaact cgtatcttac ggcggtgatt tctcgttata aggctgaacc
     2461 aaatattgag tattggcagg ttgaaaacga gccatttatc gggtttgggg cttgcccaac
     2521 actcagccaa tcagttttgg agcaagaagt tgccttggtg cgtcagcttg atccgacgca
     2581 taagattctc ctcaccgatg gaggggcttg gggaaaatgg attgaaccgg caaagctcgc
     2641 cgatgccttt ggtacaacac tctacacaaa agtgtaccgc aagggcattg gttacacccg
     2701 tccccccttc cctgctccct attacagcaa gaaagcagac attgtccgtg ccgcaacggg
     2761 tcagaaagat caaccttata tttttaccga aattggtctg gagccatggg gcggaaagtc
     2821 aattgccgaa atgtctcttg aggaacagcg gtctctccac tccttaaaag attttcgcgg
     2881 gacactcgaa tttgctcgta gtacggggtt tggcacgttt tacttatggg gcggtgaatg
     2941 gtggcattct ctcaaattca aaggtgatga tagttactgg cgtgaagcgc aaaaggtttt
     3001 tgcaagtggc cgctcgaaca atcctctagc ggatttgccc gatagcttct tatggggagc
     3061 gagtacggca tcgcatcagg tagaaggggg gactcagaat gactggacgg agtgggaaaa
     3121 ggtgcatgct aatacgcttg cggccggagc aacggagaaa ttctcccacc agataccgaa
     3181 ttggaatgcg tttgcctcag ctgcaacttc gcccacgaat tatatttcta gtgatgcggc
     3241 ggatcactat catcgttacg ctgaagattt ttcattagct cagtcgttag gtatgaacgc
     3301 gcagcgtatc tctctagagt gggctcgtat tgagccgaag cgggacgttt ttgatgcaaa
     3361 agagttagac cactaccggg cggttattgc cgatatgaaa gccaaaggaa tggaacctat
     3421 ggtcacactc tggcactaca cgctcccact ttggtttcgt gatgctggcg ggtgggaaaa
     3481 tccagagtct gcaaagtggt ttgcggaata ttcgcaaaag gtcgtatcgg agttaggtgg
     3541 cgatgttact ttttggatta ctttgaacga accgggggtt tacagtaata atgcctatct
     3601 tcttgggact cgtccgccgc aaaagcaaaa tccagttgcc tacttacgtg ttcataaaaa
     3661 tttggtccag gctcatattc aagcctacgc cgcgattaag tctgtggctg cgaatgctca
     3721 ggttggtatt gctcaagatg tgtattcata tgaatcgaac acgggtctcg tgcagcagct
     3781 tatccgtccg ctcgctgagc agtggacgaa ttattcattc ttagacagat tagggacttc
     3841 taaggatttt atcggattaa attattacat caacgttacg cttggtggaa agtcgtctcg
     3901 gactgggacg tccgatttgg ggtggtcgct ctatcccgaa ggtatctatt cggtattgaa
     3961 agggatggca cgttaccagg ttcctctcta cgttaccgaa agtggtttgg cagatgcgag
     4021 cgatactcag cgggctgatt atatggcctc ggttattgcc caaattgccg ctgctcgtaa
     4081 ggatggcgtt gatgtacgag ggtattttta ctggtcgctc attgataact ttgagtggga
     4141 aaaaggcttt tggcctcgtt ttggtttaat tgctatcgat tatacaactc aagccaggat
     4201 gcctcgcccc agcgcattca aatatgccga tctgattgcg gcgtatcgtg cgacgcatca
     4261 ataaggaggg gttgggttct cgtatgcggt gtgttatcgt acaaacgatg aaagaccgaa
     4321 tttttttata tggtgcttgg tgcgtcgcga ttatagctat gctcggtagt ctctttttta
     4381 gcgaggtgat gcaccttcct ccctgtattt tgtgttggta ccagcggatc tgtatgtatc
     4441 cgttagttct cattttagga gtgggaattt atactcgcga taagcatatt gtttcatatg
     4501 tattgccact ctcgattctt ggaatgggaa ttgcccttta tcacaatctt ctctattacg
     4561 gatttatccc agaaagcttg gctccatgcc gggcaggcat ttcgtgtacc acgaaattga
     4621 ttgagtggtg gggattcgtg accataccgt ttctgtcgtt tgttgcgttt ggactcatca
     4681 cgacagccat gtttttagtc ttaaagcagt cgaaaggaaa taatcatgca tgatggaaaa
     4741 attatcgctg gtgttattgc tgtaacagtg ctcattcttg gcggtatttt tgcgtttggc
     4801 ggcagttctg cccctaagcc ggctgatccc ctagttgtgg caagtcctga gcttatacgc
     4861 gaacatacgg cgacggtggg cggtggcgaa aaagttacgc ttgtcgaatt cggtgatttc
     4921 cagtgtcctg cttgtggttc tgcctatccg gttttaaagc aaattaaagc agattatggc
     4981 gaccgaatta cgtttgcctt tcgtaactac cccttaacga ttcacgcaaa tgctgaatta
     5041 gcggcctatg cggctcaagc ggcggccgag cagggaaaat tctgggaaat gcacgacaag
     5101 ctctttgaga cccagtctgc ttggagcctc tcatttaatc ctgaaaatac ttttgtcggc
     5161 tacgctacag ggctcggtct cgacgaggca aaattccgtg ccgatatcaa aaatcaagcc
     5221 gtgattgata aggttcgtct tgataaagga gatggtgagg ccttgaaggt gaattcaacc
     5281 cctacgctgt tcctgaatgg agcgcagatt ccaagtagct ggtcgtatga gagtctcaag
     5341 tctttgatcg accaagagtt agccaaatga accacatttt tattgccccg gctttcgctc
     5401 atgaaggtga ggttcatacc gagtcttcca ctgctcccga gccagaagta cagtatgttt
     5461 cctttagtcc cacggcaaca gttgttacgt atatcttgct cggtttgata gtgattgtcg
     5521 gtgttcttgg gttgattttt acgtataatc agcgcggtaa gcaataaaaa aaaactgccg
     5581 cctggcgaat gcgaggcggc gagttgcaaa actgaagaac gttcatctta ccagtggaat
     5641 cccacctggc cgatgagcct actggtgttg gctcctgtct ggtagcctac cttaatggtg
     5701 gcattttttg tgatgtccca gcgtggggcg acggtccatc tgtccccagc ttccagccac
     5761 tcatgctgaa ggctgaggta ggtgtgtttc gtgaccctat actcgcctaa ggcaagatac
     5821 tgagtcactc cgtccgagct aacgagttct ccgatggccg tcattcttcc tgatttatag
     5881 agcaggtcgg ccccgtgggc gggagtctgg ctaattctct ggctggtggc cagggtgagg
     5941 gccgaagtga ctcgatattc agcccggatg acgctcgaag cgcccagctc gtttccgaac
     6001 tccgggtctc ccacatacgt gccggcatac cacttggttc gtcccgtgct tccggtaacc
     6061 gttactccgg tgtctgttgc cgcgagcggc acgtagatat cagagtagta aatgaccggc
     6121 acttggtcga ctcgatagtc gtaccagatt cggccaaacg gtgggaagct cctgccaacg
     6181 gcgatctggt cgatgccctt aattggtttc ttccactgca gttctccgta cgagaatgtc
     6241 cgtagagggc cgcgcggcgt cacggacacc gtggcggtca gatctttgtt taggtctgcg
     6301 ctcacaatcc agcgcgtgtg gtcactgagc ttgttggaac tcgagtcggc cgttagcatc
     6361 caaaagccat gaaacgtaac cggttcttct gctttggctg gggctgtcca acacgagagc
     6421 agagcaataa cggcaatcca gagcacgcga ctcatcttac tcatccttta tccggaaaat
     6481 aggcgttatg cctgagaacg accattctca ctgcttaacg agtaagcagc aagaaggtag
     6541 cgctctggac cggcgaatgt cagcccagag cagagcagtt acttccatgc gtgtgccgca
     6601 atctcgcggt ggagcacgat ccggtccttc cacgatcgcc ctttcaggtc gacctggtga
     6661 ctagcttctt taatctctcc tttgtcgtcg ttcgtgatga tgccaacggt atagtacagc
     6721 cagggatgca gttgggttga ctcctctata taccttcgat acgtaggctc ggttcggcca
     6781 gcgtagacgc cttccaggaa ctctcgagta ctgatacggg gaaatacgtt gcaccacgta
     6841 aaggaggcgg tagggaagat tcgagggatg tacctcagaa aatcgggccc ttcgtcgtct
     6901 ccccagcaca tctggttaaa gaacagtatc ccctccttgg acaggtggag agaggcgtag
     6961 tggagttggc tgataaactc tctctggcca tcgctccctc cccgggcgtg ctgtgggatt
     7021 ttgagagcga tatcctcgtc attcgggtag agatgatagg gtgggtagat gccgattgct
     7081 cgacatgaac ctggtcgggc agtctgatag ccgtaggttt ccttcctgaa tcctatggcg
     7141 acatcattgg ctagcgcgtt ggacttcgca aaggcgatgg ctcggtcgct cttgtctatt
     7201 gcgagaacag gaacgttgcc ataattcttt ttcaccagga ttgcgaatac accacttcct
     7261 gtcccaacgt cgaggaaccc tatatctcct ctagatcgct tcagcgcatc ttccaaactg
     7321 gggtggatca cctcccaggt gaggggttgt tcttcgtgga acattgccat cacattgtct
     7381 gctgtgtcgg tacgatctct gttgatcggg tcaacgtgca gggtgattcc tgcaaaggct
     7441 acatttgtgt cgaacatcgt gggactgtcc atctcgcgtt tctcctctta ggccaggttg
     7501 acatttgaga atatatacga gatattacga gtggcaaatg ctacctaaga gtgataacta
     7561 tcaccacaaa aggagctcta tgtatacaga tacccttatt caacttgggc tagcccaaaa
     7621 cgaagctcgc atttatgaga cgctgcttcg agaaggcgag tcatccgtgg gtgatatagc
     7681 caccaaatct ggtgtccatc gccgtaatgt gtatgactca ctcaatcgcc tcctcgagaa
     7741 aggtctggtg tttgaaatac tcgagcgtcg ggagaaccga tatcaagcag ttgatcctgg
     7801 caaactttcc gaaatgctcc aagaaaagca gcgggcgctt gcaggtatta tgccgagttt
     7861 ggaatcactc tattctggta ccccccatcg taatgacgtg tttatctaca aaggggtgga
     7921 gtgctggaaa aactacattc ga
//
LOCUS       DASWYB010000078         6490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_761555, whole genome shotgun sequence.
ACCESSION   DASWYB010000078 DASWYB010000000
VERSION     DASWYB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761555"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VGE59_04580"
     CDS             <1..146
                     /locus_tag="VGE59_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY1074933.1"
                     /translation="PTESDMERSRISSESPVARALLGRKKGEKVTVTAPGGEVEYKIV
                     TIK"
     gene            385..1425
                     /gene="pyk"
                     /locus_tag="VGE59_04585"
     CDS             385..1425
                     /gene="pyk"
                     /locus_tag="VGE59_04585"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY1074934.1"
                     /translation="MYVKTVATIGPSSESPEVMEGLFDAGLDIARFNFSHAYPEEIYA
                     RTKCIRELNEKKGKNVVILQDLCGRRIRIGTFGEGSREIADGEEVTFYTNGAPEPLET
                     EIPINDNYLHNDLKPGDTMLIESGIFRVKITAVDTARQRITGAFEKGGKLIQKKGINV
                     PYVKLTSPAITDKDKQDILLGKELKFEYVAMSFVQSGAEIDELRSLLLPEQKIIAKVE
                     DPIGVANIDEIIQAADGIMVARGDLGVELPLEEIPFIQKEMVAKCRYAGKPSIVATQM
                     LLSMVHHPSPTRAEVSDVANAVLDGTDAVMLSDETAAGAYPVQALQTLVTVARRAEQY
                     LYQRKNNFDAKK"
     gene            complement(1477..3063)
                     /gene="gpmI"
                     /locus_tag="VGE59_04590"
     CDS             complement(1477..3063)
                     /gene="gpmI"
                     /locus_tag="VGE59_04590"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY1074935.1"
                     /translation="MNNEKVTLVICDGMGLSPEVTGNAIYAAATPTFDYLLHNYPALR
                     LLAAGTEVGLNMGEPGNSEVGHLTIGTGQVLPQAFQIINSAIKSNAYRTNAAFVTALE
                     SLRQTPEKTLHIVGLISMGGVHGHIDHIIALLQLAHDYGIQRVALHAITDGRDSPQRV
                     AVQDLMRLQPYLDQFPFGVVATIGGRYYAMDRDNSWERTDAYYWAMMGRSYTADHAGL
                     LAIQAGYNRGETDENLTPTVIQSPTGEAIAPLQAGDTVLFTNYRPDRIRQLATRVIMT
                     SLPLTVVTMTDYFLGKKAANPLPETRVLNAYPLPKPTGTLAAALSRAGKTQLHIAETE
                     KYAHITYFFNGHEEEKNSDEQWLLIPSVKIDSFARAPEMSAAPITTAYFYAREHHPAD
                     FVTINYANMDMVGHTGNFEATKQAVTYVDSQIKALVDYAEQHNEWIIFTADHGNAEQM
                     VNLQTGEIDKEHTTNPVPLIVVHPSLKQDRPIDKLQLATVGTIGILADIAPTILDILG
                     IPKPQEMVGSSLLSQFNLPH"
     gene            3147..3725
                     /locus_tag="VGE59_04595"
     CDS             3147..3725
                     /locus_tag="VGE59_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1074936.1"
                     /translation="MKTLRIALSGLSGAGSSTTAKLVAERLSLPMNNFTLRNLAVEKG
                     LSFDEIHRLAKTDSSIDIELDRRLVRFIRTHHLCFVATDLACWLDQPSLLNVLGETEP
                     LHFDYKIWLEVPLDERARRMRKREGGDISEIKTYNHARDLQNRERYIDLYGVDIFDHS
                     KIDWVFQTEKLTLSQVVESLCERILSLENPTS"
     gene            complement(3722..5485)
                     /locus_tag="VGE59_04600"
     CDS             complement(3722..5485)
                     /locus_tag="VGE59_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY1074937.1"
                     /translation="MQLRYVLLGISIFSIITFAFGVILPLFDIKPVVAFDAQSSLIWV
                     ALTAYSIVAHQLFDIRVIIKKTVVYSGLLLFTLAIYSTIIFIFTSVFGGQDALNPTLL
                     FENILAATLIAIGFEPIRRYLTNVTDKYLFKGDYDPQSVLAQLSQKLSGSLDIKEATQ
                     SLVTLVKTEMRLSHAAVVTFGEEESKIIVKQATQDGYLNPGILLLPPENLFLQHFAHS
                     PETIVTDILKSMCEGGENSNPNLQLCHILLVDLEKLSIAVAIPIKVNDKAVGMFLVGE
                     KLSGDAYTKNEIEFLQIVANQTANAIQKARFWEEDQLKSEFVSIASHELLTPTAAMKG
                     YLSMILDDGMGEIDETARKYLTKVFQSSERLAHLVEDLLNVSRIESGRLKINKKEFSL
                     VESVQKATEELSVNAKNKSLDLAFVAAPQALANVYADPDHIYRVLINLIGNSIKYTSQ
                     GWVRCFVTQQDTQHLLFTVCDSGLGIPQENLPHLFEKFYRADRKEIAGIQGTGLGLYI
                     SKKIIDLMGGQMWVESQVGKGTTFYFTLPIAGTEVAKQSEANTPHEVSALNTTGSTPV
                     QQPAPAASAVVPSPTLHAKTN"
     gene            complement(6007..>6490)
                     /locus_tag="VGE59_04605"
     CDS             complement(6007..>6490)
                     /locus_tag="VGE59_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1074938.1"
                     /translation="DILMGCMDKAFGGTGGYLCGNKKLIKYLRIACRSSILSSAITTS
                     MAGAMIESAKQIKAGVHLREELLEKATYLKNELLKAGLTVLSRDEIPAIALYIGDEEK
                     GIRFARILYENKILCPIIRWPAVPEGHTRFRIIVMVNHTKEQLDQFVEACKKAQSYIK
                     "
BASE COUNT         1762 a         1322 c         1531 g         1875 t
ORIGIN      
        1 gcccaactga aagcgatatg gaacggagcc gtatttcttc cgaatcaccc gtcgcccgag
       61 ctctcttagg ccgtaaaaaa ggtgagaaag tgacagttac cgctccaggt ggtgaagtcg
      121 aatataagat cgttactatt aaataacgag cgtttttaga gaccaaaaag ccgcatcgag
      181 cggctttttg gtttatagag ggttctctag tgaatgagac tcgtattttt tggtataaca
      241 gggtataata aatgcattgc gtctgggtga ctcagggata aatttggctg cgaaatgttg
      301 tttctgaaca tttctcgctc acatttctct cctgaagttg ctcgggcgta atcaatttag
      361 aaatagaaaa aaggagtaca gtctatgtac gtcaaaacag ttgcgacgat tggtccatcc
      421 agtgaatctc ccgaagtaat ggaaggcttg tttgatgctg gccttgatat tgctcgcttt
      481 aacttttccc acgcctaccc cgaagaaatt tatgcccgta ccaagtgtat tcgtgaatta
      541 aacgagaaaa aaggcaaaaa cgtcgttatt ttacaggatc tgtgtggccg tcgtattcga
      601 attggtacgt ttggcgaagg aagccgagaa attgctgatg gagaagaagt aacattttac
      661 accaacggtg ctcctgaacc actcgaaacc gaaattccaa ttaacgacaa ctatctccat
      721 aacgatctta agcctggtga tacgatgtta atcgaaagtg gtatttttcg agtaaagatc
      781 accgctgttg atacagctcg tcagcgtatt acgggtgctt ttgaaaaagg cggtaagttg
      841 attcagaaga aaggaattaa cgttccttac gtgaagctta cttcccctgc tattaccgac
      901 aaagataaac aagatattct tcttggtaaa gaactcaagt ttgagtacgt ggcaatgtcg
      961 tttgttcaat ctggtgctga gattgacgag cttcgtagct tgttactccc tgagcagaaa
     1021 attattgcga aagttgaaga tcccattggc gttgccaaca ttgatgaaat catccaagct
     1081 gcagatggta ttatggttgc tcgtggcgac ttaggggttg aacttcctct ggaagaaatc
     1141 ccattcattc agaaagaaat ggttgcaaaa tgtcgttatg ccggtaaacc atcaattgtc
     1201 gcaacccaga tgcttttgag catggttcat catccgtccc ccacacgggc cgaagtcagt
     1261 gacgttgcca atgctgtact tgatggtact gatgccgtga tgctttcgga tgaaaccgca
     1321 gctggtgcgt atcccgtcca agcgcttcaa actttggtta ctgttgctcg tcgtgctgag
     1381 cagtatctct atcagcgcaa aaataacttc gacgcaaaaa agtagacatt ccctgataac
     1441 agagttaaga aaagaccgtt ttccggcggt cttttcttaa tggggaagat taaactgtga
     1501 gagcaggctc gatcctacca tttcttgagg cttggggatc ccgagaatat cgagtatggt
     1561 gggagcaata tccgccagta tccctatcgt accaaccgta gcaagttgca acttatctat
     1621 tggccgatct tgtttaagac tcgggtgaac gactataagg gggacaggat tcgtggtatg
     1681 ttctttatct atctcgcctg tctgaagatt caccatttgt tcggcgttgc cgtgatcggc
     1741 cgtaaaaatg atccattcgt tgtgttgttc ggcgtagtca accaaggctt ttatttgaga
     1801 gtctacatac gtgacggctt gttttgtggc ctcgaaattt ccggtatggc caaccatgtc
     1861 catattcgca taattgatgg tgacaaaatc agcaggatga tgttccctcg cataaaagta
     1921 cgcagtggtg ataggtgctg ccgacatttc aggtgcccgg gcaaacgaat caattttaac
     1981 agaggggatc agcagccatt gttcgtcgga gtttttttct tcttcatggc cattaaaaaa
     2041 ataagtaata tgggcatact tttctgtctc agctatgtga agctgtgttt tcccagctcg
     2101 agaaagtgct gcagctaatg tcccggtagg tttggggagg ggataggcgt tcagcacacg
     2161 cgtttcagga agtgggttag cggctttctt tcctaaaaaa tagtctgtca tagtcactac
     2221 cgtaaggggg agcgatgtca taattactcg agttgcgagc tgtcgaatcc ggtctgggcg
     2281 gtagttcgta aataaaaccg tatcccctgc ttgaaggggg gcaattgctt ctccggttgg
     2341 gctttggata actgtcggcg tgaggttctc gtcggtttct ccacggttgt atcctgcttg
     2401 gatagccaga agaccagcgt gatcagcggt atatgatcgt cccatcatgg cccagtagta
     2461 agcatcagtc cgttcccaag aattatctcg gtccatggcg tagtaacggc ctccaatcgt
     2521 tgccacgact ccaaaaggaa attggtctaa gtagggttgc agccgcatta agtcttgtac
     2581 agcgacacgc tgggggctat cgcggccgtc agtaatggca tggagagcga ctcgttgtat
     2641 cccatagtcg tgggcgagct gtaaaagagc gattatatgg tcaatgtgac cgtggacgcc
     2701 gcccatcgaa ataaggccca caatgtggag tgttttttct ggtgtttgac gtaggctctc
     2761 taaggcggtc acgaaggcag cgttagtccg ataggcgtta cttttaattg cgctattaat
     2821 aatctgaaag gcctgtggga ggacttggcc cgtaccaatc gtaaggtgtc cgacttcgct
     2881 attgccgggc tcacccatat taaggccgac ttcggttcct gcggccagca aacggagagc
     2941 cgggtagtta tggagaaggt agtcaaaggt tggggttgcg gctgcgtaaa ttgcgttacc
     3001 ggtaacctcg ggcgagaggc ccataccgtc acaaataacc agtgtaactt tctcattatt
     3061 catatatcta tcctaccata tctgactctg gcggagggga agagggttgc ccttactgct
     3121 tattctcggt acaatcggta gtatctatga aaacactacg gattgcacta tcgggtttaa
     3181 gcggtgcagg aagttcgacc actgccaaat tagtcgccga gcgtcttagt ttgccgatga
     3241 ataactttac cttgcgtaac cttgcagtag aaaagggact ttcttttgat gagattcatc
     3301 gtctggcgaa aactgactct tcgattgata tcgaacttga tcggcgatta gttcggttta
     3361 ttcgtactca ccacctttgt tttgttgcta ctgacttagc ctgttggcta gatcagcctt
     3421 ctttattgaa cgtgctgggt gagacagagc ctctccactt tgattacaaa atctggcttg
     3481 aagtgccgct agacgagcga gcgagacgga tgcgaaaacg tgagggggga gatatttccg
     3541 agattaaaac ctataaccac gcccgcgact tacaaaatcg cgagcggtat attgatttgt
     3601 acggcgtaga tatttttgat cactcgaaga tcgattgggt ttttcaaacc gagaaactta
     3661 cgctttctca ggtggtagag tctctttgtg agcgaatcct gtctctcgaa aatccaacga
     3721 gctaattcgt ttttgcatgc agagtgggtg aaggtacgac ggcactagct gctggagctg
     3781 gttgttgtac gggagtcgat ccggttgtgt ttaatgcaga gacttcatga ggtgtgttcg
     3841 cttcgctttg tttggcaact tcggtgcccg caatgggtaa cgtaaagtag aatgttgtcc
     3901 ctttgccgac ttggctttca acccacatct ggccgcccat aaggtcgata atttttttac
     3961 taatatacaa tcctagcccc gtaccttgga taccggctat ctctttacgg tctgcccgat
     4021 aaaatttttc aaacaagtgc ggtaaatttt cttggggaat cccaaggcca gagtcacaga
     4081 cggtaaagag caaatgctgt gtgtcttgtt gcgttacaaa acagcgcacc cagccttgag
     4141 aggtgtactt aatagagttt ccaattaggt taataagaac ccgataaatg tgatcaggat
     4201 ctgcgtacac atttgctaga gcctggggag cggcaacaaa cgctaagtcg agtgatttgt
     4261 tctttgcgtt cacactgagc tcttcagtgg ctttttgtac gctctcaacg agagaaaatt
     4321 cttttttgtt aatctttaat cggccacttt caatacggga cacattcaaa agatcttcga
     4381 caaggtgggc taaacgttca ctcgattgaa aaacttttgt aaggtattta cgggctgttt
     4441 catcaatttc acccatgcca tcgtcgagaa tcatcgagag gtagcccttc atagctgcgg
     4501 tcggggtaag aagctcgtga gaggcaatgg acacaaattc cgatttaagc tggtcttctt
     4561 cccagaagcg agctttttga atcgcgtttg cggtttggtt ggcgactatc tggagaaatt
     4621 caatttcatt ttttgtgtac gcatcgcctg agagtttctc accaaccaag aacatgccga
     4681 ctgccttatc attcacctta atggggattg caacggcaat actcagtttt tctaaatcga
     4741 ctaataggat atggcaaagc tggaggttgg gattactatt ttctccgcct tcgcacatac
     4801 tcttgaggat gtcggttaca attgtttcgg gactgtgagc gaaatgctga aggaagaggt
     4861 tctcgggtgg gagtaaaaga attcccggat tgagataacc gtcctgagtc gcttgtttca
     4921 caatgatttt tgattcttct tccccgaatg taacgactgc tgcatgactt aagcgcattt
     4981 ctgtctttac aagtgttact agcgactgtg tcgcttcttt aatatcaagt gatccagaaa
     5041 gcttttggga aagctgagct aatacagatt gaggatcata gtcgcccttg aaaagatatt
     5101 tatcggtaac gtttgttaag taacgtcgaa taggctcaaa tccaatagcg atcagggttg
     5161 ctgctaaaat gttttcgaat aataacgtag gattaagcgc atcttgaccg ccgaatacag
     5221 aggtgaaaat aaagatgata gtactataaa ttgcaagtgt gaaaagaagt agtccagagt
     5281 agacgacggt ttttttgata atgacacgga tatcgaacag ttgatgggct acgattgagt
     5341 aagctgtaag cgctacccat ataagagaac tctgagcatc aaaagccact actggtttta
     5401 tgtcgaataa aggtaaaata actccaaacg caaaagtaat aattgagaat atagaaatac
     5461 cgagaagtac gtatctcagc tgcatacgct ttatccctct tgcatggata aatgcgcttc
     5521 ccagcttata taagagatat acaagaagag ctattagaat tccgctgtag tagtcgaaaa
     5581 gtaggccagt ttgaaaatcg tattgtccgt ttcctagcgg ttttacagat tcaataatat
     5641 aaccgtttat aaaaggaatg ataaaaaaga tcagtccaag agaggagatg atttttactt
     5701 tagtagattt tttttgaggc tctgtgaaat aaagcgtcca aataatagcg ctaattaata
     5761 aggctcctcc aaatgcgtat tgtgatctaa agatcattat acttggttga aagacaagaa
     5821 aatggaagtt agtatatgac caaagtgcgt cgattaaagc aaaaacaaaa aatgaagtga
     5881 aaagcggttg acgctttccc cgggtaataa ctagtagtcc taatccaaaa ctcgcgactg
     5941 acgcgattaa cgtcaggtaa ttaaggaagt tcattactat tattataaag caaaatcagt
     6001 ttaatgctat tttatatacg attgagcttt tttacatgct tccacaaatt gatctaattg
     6061 ttcttttgta tggttaacca ttacgatgat tctaaatcgc gtatgacctt cgggcactgc
     6121 tggccatcta attataggac agagtatttt attttcataa agaatacggg cgaacctaat
     6181 acctttttct tcatctccaa tatataaagc gatagctggt atttcgtccc gggataaaac
     6241 cgttaatcct gcttttagta attcattttt tagatatgta gccttttcaa gtaattcttc
     6301 tcttaagtgt acccctgctt ttatttgttt tgcagattcg atcatcgcgc ctgccataga
     6361 agtcgtgata gcagaagata aaattgaaga acgacaagct attcgcaagt acttaataag
     6421 ctttttatta ccgcataagt atcccccagt accgccaaaa gctttatcca tgcaacccat
     6481 gagaatgtcg
//
LOCUS       DASWYB010000079         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_610450, whole genome shotgun sequence.
ACCESSION   DASWYB010000079 DASWYB010000000
VERSION     DASWYB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610450"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2046)
                     /locus_tag="VGE59_04610"
     CDS             complement(<1..2046)
                     /locus_tag="VGE59_04610"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEY1074939.1"
                     /translation="MVLRWGFVVLFFALWQPTGLAQAAETTLTPTADTTVVYSAPSQS
                     FGSEAVVSALYQGGTDLTNTPEEVKEGLLKFDLSSLPKGRTIQSAKLVLKGSGASVTG
                     YGDIRLRRILETWDESTTWATRPKYSAESEANLEVDHNVFPSEIDLTALVRGWYTGSY
                     ANNGVVMRAFGDGDYRTGFYSSDDAHISSKEGGNPAQLIIRYSESGTVSSTSSPRPST
                     TSTPTSLSAGTSTPTPVTTATVTATAVSTPASTATPLSEFFRKLSPCMPEISRLEISL
                     GGDSATVHAILTRSLPLNVYFYTSSKAGPNKLATDYESSQVIKSAASASHSLVLPGLN
                     PGTTYYFTSVVNGKQDCEQSFALLTLTEKGAGPSLLSGSSHGLSAETKSSNPGPGIAS
                     LTILAAPFRVVGHLIFGDAGASPLIWGPFLLAILFWGTLTRGALHRLSLLIKWWRNLP
                     NLRYALTHPVKKGSTWGRVMSSLTMQGISRARIWLLNGKTGAIVAQTSSDEHGFFGFS
                     APAGSYKVKVTHGAYAFPSAIMPFGYRGDLVRVEGANKVVQLDLPMDPAELTDRSFQR
                     MKGWLERFALLRLPLLLILTVFLGLSFLERTTTLQFLAVLLVAYLWWEEWKNIHDAKI
                     LARLVDAQNKAVPFALVHFKNSYGRVYSRSVTDNHGEFRAIVPAGVYSLHIITFHQND
                     "
     gene            complement(2046..2438)
                     /locus_tag="VGE59_04615"
     CDS             complement(2046..2438)
                     /locus_tag="VGE59_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074940.1"
                     /translation="MTRPPGYVALLTVIMVTVILTLIGVTAAFRSLGSRQTELVGSFK
                     QQSKYAAEGCLDQALLNLSLNNTYAGNETITYPESGTTCKIEPIYLVTSQKILKASSQ
                     VREASTYLVLTVDAVTLERISLQEVRGF"
     gene            complement(2435..>2891)
                     /locus_tag="VGE59_04620"
     CDS             complement(2435..>2891)
                     /locus_tag="VGE59_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074941.1"
                     /translation="LYSGLVVIILGITITASLTFTNASLRDKKRSEVVANERFVREKS
                     DWLFSGLESNDVITPLTSGGTLDATLSSGQRIRLQLTGTTLELVRDTNGDGAITAADT
                     KSALTNQYVTVSDLTFARETWNGQPTFVMTCRMASENFSLNFQKRVYLQ"
BASE COUNT          818 a          703 c          680 g          690 t
ORIGIN      
        1 gtcgttctgg tgaaacgtta taatgtgcaa actatacact ccagccggaa cgatcgcccg
       61 aaattcacca tggttgtcag taacacttcg agagtaaact cgaccatagc tgtttttaaa
      121 gtggaccaaa gcaaagggaa cagctttatt ctgagcatcg accagccgag ctaaaatttt
      181 ggcgtcgtgg atatttttcc actcttccca ccataaatag gcaacgagaa ggaccgccaa
      241 aaactgcaat gtcgtcgttc tttccaagaa agaaagccca agaaaaaccg ttagtatgag
      301 caaaaggggc aatcgaagaa gggcaaatct ttccaaccat cctttcattc tttgaaaaga
      361 acgatctgta agttctgctg gatccattgg taagtcgagc tgaacgactt tattagctcc
      421 ctcaacccga acgaggtccc cacgataccc aaatggcata atggcggaag gaaaggcata
      481 cgcaccatga gtaaccttca ccttgtaact gccagcgggt gcagaaaaac caaagaatcc
      541 gtgttcatcc gacgacgtct gagcaacaat tgcgcccgtt ttaccgttca gaagccagat
      601 gcgagcgcgg ctaataccct gcattgtgag tgatgacata acccgacccc aagtcgatcc
      661 ttttttgaca gggtgagtaa gagcatatcg taagttgggt aaattccgcc accatttaat
      721 gagaagcgat aaacgatgca aagcccctcg agttaatgta ccccagaata aaatcgctaa
      781 gagaaatgga ccccatatga gtggagaagc gcccgcatct ccaaagatca aatggccaac
      841 cacgcggaag ggagccgcaa gaatagtcag cgaggcgatt cccggtcctg ggttagagga
      901 ttttgtctcg gcacttaagc catgacttga cccacttaaa agtgagggac ctgcaccttt
      961 ttcggtcaat gtaagtaatg caaaactttg ctcacaatcc tgcttcccat taacgacgga
     1021 ggtaaagtag taggttgttc ccggattaag ccctggcaaa actaggctat gcgacgcact
     1081 ggcggcgctc tttattactt ggcttgattc ataatctgtt gctaatttat ttgggccagc
     1141 cttagatgag gtgtaaaagt acacgtttaa aggaagtgag cgcgtaagta ttgcgtgaac
     1201 cgttgcacta tcaccgccta aagaaatttc taaacgagag atttctggca tacacggtga
     1261 tagcttgcga aaaaactccg agaggggagt agctgtggag gcgggggtag aaacagctgt
     1321 agcagtaact gtcgccgtag tgactggcgt gggggtcgaa gttcccgcac ttaaagaggt
     1381 aggcgtagac gttgtggaag gacggggaga tgaagtactt gaaaccgtcc cactctcact
     1441 gtaacgaata ataagctgag cagggttacc gccttcttta gaagatatgt gagcatcatc
     1501 acttgaataa aaacctgtcc gataatcacc atctccaaaa gctcgcatca cgacgccatt
     1561 atttgcatac gatcctgtat accacccccg cacaagtgcc gtgagatcta tttctgaggg
     1621 aaagacatta tgatcaactt ctaaattggc ttcactctca gctgaatatt tgggtcgcgt
     1681 agcccaggta gttgattcat cccacgtttc aagaatacgg cgcaagcgga tatctccata
     1741 ccccgtcact gaggcgccac tccccttaag aacaagtttt gccgactgga tagttcgtcc
     1801 cttcggaagg cttgataagt caaattttaa gagaccttcc ttcacttctt cgggggtatt
     1861 tgtcagatca gtccctccct gatagagcgc cgagactacg gcctcggagc caaaagattg
     1921 gctcggcgcg gaatagacta ccgttgtgtc agccgtgggc gtaagggttg tttcagctgc
     1981 ctgcgccagc cctgttggtt gccacagtgc gaaaaagagt acgacaaatc cccagcggag
     2041 aaccatcaaa acccccgcac ttcttgaaga gaaatccgct cgagcgtaac agcgtcgaca
     2101 gtgagaacta agtaagtcga cgcttcgcgt acttgcgatg aggccttaag aattttctgc
     2161 gaggtaacta aataaatcgg ctcaatcttg cacgtagtac cagattcagg ataggttatc
     2221 gtttcattcc ccgcataggt gttattgagc gaaagattga gcagagcttg gtctaaacaa
     2281 ccttcagccg catatttgct ttgctgctta aaagatccaa ccaactcagt ctgacgcgac
     2341 ccgagcgaac gaaacgctgc ggtcacgcca atgagcgtaa gaataacagt taccataata
     2401 acagtcagga gggcaacgta accgggcgga cgcgtcattg gaggtacact cttttctgaa
     2461 agtttagtga aaagttctca ctcgccatac ggcacgtcat aacaaaagta ggctgaccat
     2521 tccacgtttc tcgggcaaac gtaagatcgc tgacagtaac atactggttt gtaagcgctg
     2581 acttcgtatc ggctgccgta atagcgccat caccgttggt atcccgcact aactcaagtg
     2641 tcgttcccgt tagctgaaga cgtatacgtt gcccactact gagagttgca tccaaagtcc
     2701 cacccgaggt aagaggagta ataacatcat ttgattctag tccagaaaaa agccagtctg
     2761 atttctctcg gacaaaccgt tcgttcgcca ccacttcgct ccgtttttta tcgcgtaaac
     2821 ttgcattggt aaacgtcaaa ctggcagtaa tcgtaatccc caggattatt actactaatc
     2881 cactatagag g
//
LOCUS       DASWYB010000080         8027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_610694, whole genome shotgun sequence.
ACCESSION   DASWYB010000080 DASWYB010000000
VERSION     DASWYB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610694"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1436)
                     /locus_tag="VGE59_04625"
     CDS             complement(156..1436)
                     /locus_tag="VGE59_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074942.1"
                     /translation="MTHQNKLIVWVAGSVLLLAVVIGTVFFVRTGSSRPESTTADSSL
                     NTGDESSDMALSSSANNGDEFKRQYGNTCDKNANPTFTHSPLSVDKIGGIEPLGRVSD
                     GHVTPVDHVYVSPIDSTVADNTYDVVMPADGTLVDVGRMPDQYIGDRSDVKLAQDDFR
                     MVISFSCRYYSIFIHVHKLSDTVAAAVGTIKPNETKRISLKLKAGDVLAHLGGSSFDW
                     TMVDTQTNLTGFITPSYYESEPWKIHTIDPFSMYSGSLKTSLEAKSLRTVAPFGGKID
                     WDKQGALIGNWYKEGTKGYQGASQDRYWDGHLAIAPHNIDPTGVIYSVGNWNGKAGQY
                     LVKGDFKPEEITAESGIVKIELMNITMKNPDGNPFSGGVFKRGMILDKSSSPVVGVVL
                     LQVQAGEKLKLEQFPGKTAAQVSGFTPAAVTFER"
     gene            complement(1531..2004)
                     /locus_tag="VGE59_04630"
     CDS             complement(1531..2004)
                     /locus_tag="VGE59_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY1074943.1"
                     /translation="MKLNWVRLLACVVGTNIVGNIGSLATFSQITTWYATLEKPWFNP
                     PNWLFGPAWTLLFTLMGISLYLILSHAGKREMKTAVLVFSIQMVLNVLWSFIFFGWHQ
                     LGIAFAEIVILWVAILVTIINFSRISKVAAWILVPYLGWVTFASLLNFAVWQLSR"
     gene            complement(2001..2405)
                     /locus_tag="VGE59_04635"
     CDS             complement(2001..2405)
                     /locus_tag="VGE59_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1074944.1"
                     /translation="MKGYKANIEQLTLENENFRQVLYTAKHVQLVVMNLKPGEEIGLE
                     VHDVDQFFRFEKGEGKVVIDGTEQMVSDGDVVIVPSGAEHNVVNASSSESLKLYTLYC
                     PPHHQDGTVHSTKEDALTDDEHFDGTTTESVL"
     gene            complement(2522..2914)
                     /locus_tag="VGE59_04640"
     CDS             complement(2522..2914)
                     /locus_tag="VGE59_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074945.1"
                     /translation="MNYTGVIIEESLRDSSLLKDFKITGTEIEIVNENFKTPWLTQWT
                     LHTIEIEEDDIDRVADQISRSFDRDHSDWYADFKNDVSHYIIFPNKVFKVDCGNVENY
                     RPAVKHGFDLGIPIYQLNFKPEIEKWQF"
     gene            3140..3343
                     /locus_tag="VGE59_04645"
     CDS             3140..3343
                     /locus_tag="VGE59_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074946.1"
                     /translation="MDNKEFNKAFDEATKKKMLEEPDIWFKPRRQRWDEWGSPVGLSL
                     GWALFIVPLGVFLWLLHLAGLIG"
     gene            complement(3460..3939)
                     /locus_tag="VGE59_04650"
     CDS             complement(3460..3939)
                     /locus_tag="VGE59_04650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1074947.1"
                     /translation="MELIDYFDFEAVVLERIQDSDIDESVTLINHAYKYQEQYKNEPR
                     VNRETLITQVKKSKFYVAKSNNHVIATVYIQPKGETLKLGLLAVLDAYRGKGLAPAII
                     KAIEEYGRTGGFKSLALDYMSIAPWLKKYYEKYGFKETGEFENWGTIDLIHMEKSLC"
     gene            complement(3940..4449)
                     /locus_tag="VGE59_04655"
     CDS             complement(3940..4449)
                     /locus_tag="VGE59_04655"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1074948.1"
                     /translation="MNKRFLLLLDGMTGAGKTTTTKFLAAELPRTAVIGMDKVKKFVS
                     DFERGERDNTIAREVVFIMAQKYLELGLSVIIDQPFWDAADVRRYEDLAASQSIPCYK
                     FQLFASPQVAYARVMERQKEWDDKVPEERVKSNIEYFKKNDDLGFTLIDTSHLESDMA
                     AKMIMREIG"
     gene            complement(4573..5001)
                     /locus_tag="VGE59_04660"
     CDS             complement(4573..5001)
                     /locus_tag="VGE59_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1074949.1"
                     /translation="MSTLHPWINFNGNAEEAFTFYKSVFATEFTKVVRFKDIATDEFP
                     VAPKEENKIMYIALPIGNGSMLIANDVPEVLGRVNEQENRSKILVRTESKEEADRLFD
                     GLSAGGDVEGPMGDSPWGSYAGMFRDKYGIEWIIEFSPAK"
     gene            complement(5114..5590)
                     /locus_tag="VGE59_04665"
     CDS             complement(5114..5590)
                     /locus_tag="VGE59_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY1074950.1"
                     /translation="MTISFDKLSEIRKKHSHERIVLGSGVFDLLHWGHIPYLKALKKY
                     GDIVVVGVMDDAGVRHKKGLQRPVLAQDHRVLLVDSIKGVDYTFVAPFDPTDLAYTKI
                     FRHLKPDVFYTTNPRWETFKELEDINIIIGDRYVPGYPASTTDIISEIIKKSSAEF"
     gene            complement(5767..6321)
                     /locus_tag="VGE59_04670"
     CDS             complement(5767..6321)
                     /locus_tag="VGE59_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY1074951.1"
                     /translation="MKEKDILILIEQDIWMMRILEITRDLNLPDWWIGAGFVRNKVWD
                     HLHGYEERTPLADVDVIYFDPSNLDEAVEDAIQDKLKKIDSTITWSVTNQARMHIPSN
                     EPPYKSSEHALSLWPETPTSVAVTLNDKGGVALIAPHGIQDLVTLTVNPTPHWRKKPG
                     DYESRLAKKKWDEKWPKLIINHLS"
     gene            complement(6376..6450)
                     /locus_tag="VGE59_04675"
     tRNA            complement(6376..6450)
                     /locus_tag="VGE59_04675"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6416..6418),aa:Asn,seq:gtt)
     gene            6609..6899
                     /locus_tag="VGE59_04680"
     CDS             6609..6899
                     /locus_tag="VGE59_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY1074952.1"
                     /translation="MEKMLVITRHRFEPQELRVTRGETITIRNESRENHTVTADDGIF
                     DSDIIGPTEEIIMEIPDDIPLGVNTYHCSIMEDMKGVIVVRESKDETRNNLA"
     gene            complement(6950..8017)
                     /gene="rpoD"
                     /locus_tag="VGE59_04685"
     CDS             complement(6950..8017)
                     /gene="rpoD"
                     /locus_tag="VGE59_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY1074953.1"
                     /translation="MPADFPESLKRLVDKGRQFGFVTYQEILQSFPTIEDDIILLDRL
                     YELLASRGIEVRESKSLLQLPNSEPNKKDVKDDELGDISDDSVRMYLREIGHYPLLSG
                     DEEVSLAKRIMKGDTNAKMRLAQANLRLVVSIAKKYIGRGLSLLDLIQEGNMGLMRAV
                     EKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINRLIREQRRLVQEL
                     GREPSPEEIAKAMKIPVDKVEHIMKISQETVSLEAPVGEEEDSALGDFIPSTEHGPEE
                     QAIYQLLRDHVSEFLQYLSPREQKILRMRFGLDDGRTHTLEEVGEEFGVTRERIRQIE
                     AKALQRLKKHEESHKLQDYLK"
BASE COUNT         2298 a         1885 c         1540 g         2304 t
ORIGIN      
        1 gattataccg ggtattaccg aaaaagcacc ttaacggtgc ctattcggaa gatctgcttc
       61 ttgtaaatca gccacaatga tcttcttctc caccaacgaa gcacattttt tattaacaaa
      121 gaggagctga gattgctcct ctttgttgct aactattatc gttcaaatgt gacggcagca
      181 ggggtaaaac cacttacttg agctgctgtt tttccaggga attgttctaa cttcaatttt
      241 tcaccagctt gcacttgaag taagacaaca ccaactaccg gactacttga tttatcaagg
      301 atcattccac gtttgaagac gccaccgcta aatggatttc catctgggtt tttcatcgta
      361 atattcatta attctatctt aacgatccca ctttcagcag taatctcttc aggcttaaag
      421 tcacctttca ccaaatactg tcctgctttg ccgttccaat tacccaccga ataaatcaca
      481 ccggtcggat caatattatg aggcgcaatc gctaaatgcc cgtcccagta gcgatcctga
      541 gaagctcctt gatatccttt ggtaccttct ttgtaccagt taccaatcaa tgcaccttgt
      601 ttatcccagt caattttacc gccaaatggg gcaaccgtac ggagactctt tgcctcgagg
      661 cttgttttaa gagaacccga atacatgctg aacggatcga tggtatgaat cttccatggt
      721 tcagattcat agtaagacgg ggtaataaat ccggttaagt tggtttgtgt atcaaccatc
      781 gtccagtcaa acgagctgcc tcctaaatga gctagcacat cgccggcttt taacttcaag
      841 gaaatacgtt tcgtttcatt gggttttatt gtgccaactg cagcggcaac tgtgtcactt
      901 aacttatgaa cgtgaataaa tatggaatag taacggcagc taaacgaaat aaccatacga
      961 aaatcgtcct gcgccagctt gacatccgac cgatcaccga tatattgatc cggcatacga
     1021 ccgacatcaa ccaaagtacc atccgctggc atgacaacat cataggtatt atcagcaact
     1081 gtagaatcaa tgggggagac atatacgtgg tcgacgggag taacgtgacc atcagacact
     1141 cttcccaacg gttcaatccc accaatttta tctaccgaga ggggcgagtg agtaaatgtc
     1201 ggattagcat ttttgtcgca cgtgttacca tactgacgtt taaactcatc accgttattc
     1261 gcagatgacg acaaggccat atcgcttgat tcgtcacccg tatttaagct cgaatcagcg
     1321 gttgtacttt ccgggcgact ggaacctgta cgaacaaaaa atacggtgcc tattaccacc
     1381 gccagtaaga gaacagaacc agctacccaa acaataagtt tattttgatg tgtcatatat
     1441 gcagcatagc agtataatca tttcttacac aacaaacacc gccgatgaca cctcaataac
     1501 atgtcctatt cacatcacgg cttacaaaac ttagcgactg agctgccaaa ctgcaaagtt
     1561 aagcagagaa gcgaatgtta cccagccgag gtacggaacg agaatccatg ctgccacctt
     1621 tgagatcctt gagaaattaa tgatcgtaac caagatcgca acccaaagta taacaatctc
     1681 agcaaacgca atcccaagct gatgccaacc aaaaaagata aaagaccaca atacatttag
     1741 taccatttgg atgctgaaaa ctaggaccgc tgttttcatt tctcgcttac ctgcgtgaga
     1801 caaaattagg tacagagaaa tccccatcag ggtaaaaagc aatgtccacg cagggccaaa
     1861 caaccaattc ggaggattaa accaaggttt ttcgagcgtg gcataccatg tcgtaatttg
     1921 gctaaacgta gcaagtgaac caatattacc aacaatgttt gtaccaacta cacaggcaag
     1981 aagtcgaacc caatttaatt tcacagtact gactccgttg tcgtcccgtc aaaatgctcg
     2041 tcgtccgtga gtgcatcttc tttggtcgag tgcacagttc catcttggtg atggggcgga
     2101 caatagagtg tatacagctt aagcgattca gacgaagagg cgtttacgac attgtgttca
     2161 gcaccggagg gcacgatgac gacatcgccg tccgaaacca tttgctccgt accatcaata
     2221 acaacctttc cttccccttt ttcaaaccgg aagaattgat caacgtcgtg aacttctaag
     2281 ccaatttctt caccgggctt taagttcatt actacaagtt gtacatgctt cgcagtgtag
     2341 agcacctgac gaaagttttc gttctcaagc gtcaattgct caatgttggc tttataccct
     2401 ttcatatgac tccttttatt atttatctac tctccatata taacacttac ttcagaaaaa
     2461 gcagacttcg tcttccggat tacccgtgat aatgtcaatt ttagtttatt acgtcgattc
     2521 tttaaaactg ccacttttct atttcaggtt taaagttaag ttggtaaatt ggaatgccca
     2581 gatcgaagcc atgttttacg gctggccggt aattctcgac gttgccgcaa tcaaccttaa
     2641 aaaccttgtt gggaaaaata atgtagtgag agacatcatt cttgaagtct gcataccaat
     2701 cggagtgatc tcgatcaaac gaacgactta tctgatctgc tacccggtca atatcgtctt
     2761 cctctatttc aatcgtatgg agtgtccact gcgttagcca tggcgtttta aagttttcat
     2821 tcacaatttc gatttcagta cctgtaattt taaaatcttt aagtaaagag ctatctctaa
     2881 gactttcttc gataataaca cccgtgtagt tcatgcgtgt attttagcac aataaaaata
     2941 gttacgaatc acgaattgat ctggcatttg tttttatact tggatatgtt atgaaattat
     3001 tgattggttt tcatacttct gtcctgccct tgggatatat caaaagaagt ccgatcgtaa
     3061 atcaagggcc ccaaactatc tatgtccctg tacgggaggt cgaactggat agtcaatcaa
     3121 ccaacatctt tggagaatta tggataacaa agaattcaac aaagccttcg atgaagctac
     3181 gaagaagaaa atgcttgaag agccagacat ctggtttaaa ccacgaagac agagatggga
     3241 tgaatggggc tcgccggtcg gactatccct cggctgggcg ctctttatcg tcccgcttgg
     3301 tgtgtttctc tggttacttc acctcgccgg cctcatcggc taagcataaa aaacgtcccg
     3361 aatgtatcgg gacgtttttt tgttcactca ctataaaaac cctgacctgt ttttcacgga
     3421 tattactttt ctacgcctta gtcttaaatc tatctgaccc taacataaag acttctccat
     3481 atgtataagg tctattgttc cccaattttc aaattcaccc gtctctttaa atccgtactt
     3541 ttcataatac tttttcagcc atggagcaat cgacatatag tcgagcgcta aactcttaaa
     3601 accacccgtt cgaccgtact cttcaatcgc ttttataatc gccggtgcaa gacctttacc
     3661 acggtaagca tcgagaaccg cgagtaaccc tagcttgaga gtctctcctt ttggctggat
     3721 gtagacagtc gcaataacgt gattattact ttttgcgaca taaaatttgc tctttttaac
     3781 ttgagtgatt aacgtctccc gattaacacg aggttcattt ttgtattgtt cttggtattt
     3841 ataggcgtgg ttaataagcg taaccgattc atcgatatca ctatcttgaa ttctctctaa
     3901 tacaaccgcc tcaaaatcga aataatcaat aagttccatt tatccaatct ctcgcataat
     3961 catttttgcg gccatatctg attctaaatg tgatgtatct atcaaggtaa atcctaagtc
     4021 atcatttttc ttgaagtact caatattgct tttaactcgt tcctccggca ctttatcgtc
     4081 ccattctttc tgacgttcca tcactcgcgc gtaagcaact tgaggagatg caaagagttg
     4141 aaacttgtaa cagggaatcg attggctcgc agccaggtcc tcgtagcgcc gaacatcggc
     4201 ggcatcccaa aacggctgat caataatcac tgacaaccct agttctaaat atttctgagc
     4261 catgataaac acaacctcgc gggcaatcgt gttatctcgt tcaccccgct caaaatcaga
     4321 aacaaacttt ttcaccttat ccataccaat aacggccgtc cgaggtaact ccgccgctaa
     4381 aaactttgtt gtcgttgttt tacccgctcc tgtcattccg tcaagtaata acaaaaatcg
     4441 tttattcatt aaataaatta tactcctctt taaggaaaaa gcagccttac ggccgctctc
     4501 tccatgtgat agatgaaata aaaaactgct ttgcactaat gcactacgtg aaacaatatt
     4561 gcgaaggcta ttttatttag caggagaaaa ttcaatgatc cactcaatgc catacttgtc
     4621 ccggaacatt ccggcatagg aaccccatgg gctatctccc ataggtcctt ctacgtctcc
     4681 tccggccgaa aggccatcaa ataatctgtc cgcttcttct ttactctccg tacgtaccaa
     4741 tattttgctc cggttttcct gctcgtttac gcgccctaaa acctccggca catcattggc
     4801 aatcagcata gaaccattac caatcggtaa cgcaatatac attatcttat tttcttcttt
     4861 tggggccacg gggaattcat cagttgctat atctttgaaa cgaaccacct tcgtaaactc
     4921 agtagcaaaa acagacttat aaaacgtaaa tgcttcttca gcgttaccgt tgaaattaat
     4981 ccagggatgt aatgtagaca tgcagcaata atagcaagga aaggagtaaa attctacgtt
     5041 tcaccaacat acagccaaac aaaaacccgc cttttaaggc gagctctctg ctggccccga
     5101 gttctagaaa gcgttagaac tcagcactac ttttcttaat aatctcacta ataatatctg
     5161 ttgttgaagc aggatatcca ggtacatatc ggtcaccaat aataatattg atatcttcta
     5221 actctttaaa tgtttcccag cgtggattcg tcgtgtagaa gacatcgggc tttaagtgac
     5281 gaaatatctt cgtataggct agatccgttg ggtcaaaggg cgcaacaaac gtgtaatcaa
     5341 ccccttttat agagtcaaca agcaaaactc gatgatcctg cgccaagacc ggcctttgta
     5401 gccctttctt atgtcgaacc cctgcatcat ccatcacacc taccacaaca atatctccgt
     5461 acttttttag cgctttaaga tacggaatgt gcccccaatg gagcaggtca aaaacaccac
     5521 tccctaacac aatcctttcg tgcgaatgtt ttttacgaat ttccgaaagt ttatcgaaag
     5581 atatcgtcat ttcttttcct cttatattct cgttaaatta tatgtccttc tactcttaag
     5641 catagcactc cataccaaaa aagcacccgt atggatgctt ctttggttct aactctctgc
     5701 tagataatca atttttacct ataaagtcgt tggctccgaa ctgcagacca tctccggact
     5761 caaacattag cttaagtgat taattattaa ctttggccat ttctcgtccc actttttctt
     5821 ggccagacgg gattcgtaat ctccaggctt ttttcgccaa tgcggagtcg gattcacagt
     5881 tagcgtcacc agatcttgta tgccgtgcgg ggcgatcaat gcaactccac ccttgtcatt
     5941 cagtgtaacg gcaacacttg ttggtgtttc tggccacaac gatagggcat gttcactcga
     6001 tttatagggc ggttcgttgc ttggaatatg catccgagct tgattggtaa cagaccaggt
     6061 gattgtggaa tcaattttct ttaatttgtc ttggattgcg tcttcgacgg cttcgtccag
     6121 attactggga tcaaaataga taacatcgac atcagcaagt ggcgttctct cttcgtagcc
     6181 atgcaaatga tcccaaactt tattgcgcac aaagcccgcg ccaatccacc aatctggtaa
     6241 attgagatca cgcgtaattt caagaatacg catcatccat atgtcctgct cgatgagaat
     6301 tagaatatct ttctctttca tgatgtcact ataacaaaag aatacgaaaa agcagccttt
     6361 cggccgctac ttcgttggct ccggaggtcg gattcgaacc gacgaccaag ggattaacag
     6421 tcccctgctc tgccgctgag ctactccgga ataatgtatt attaggtaca cagatatttt
     6481 accaattgta tccacacctg taaatacaat ttacggactg atccgacaca aacataagaa
     6541 gatactgcgc aaaatgaata ttgcccgcct aactaagata tgtaatggta aggctgcaaa
     6601 ggagccaaat ggaaaaaatg cttgttatta ctcgtcaccg ttttgaacca caggaactac
     6661 gagttacacg cggggagact attacgattc gtaatgaatc ccgagaaaac cacaccgtta
     6721 cggcggatga tgggatattt gacagtgata ttattggacc gacagaagag attataatgg
     6781 aaatccctga cgacattccg cttggagtaa acacgtatca ctgttctatt atggaagata
     6841 tgaaaggtgt tattgttgta cgagaaagta aagacgaaac aagaaacaac ttagcctaaa
     6901 agaaaaaacg cccatccggg cgtttttatt tatagttacc gacccaaaac tatttaagat
     6961 aatcttgtaa cttatgtgac tcttcgtgtt tcttgagtcg ttgcaatgct ttggcttcga
     7021 tttgacgaat acgttcgcgg gtaactccaa actcttcacc cacttcttcg agcgtatgtg
     7081 tccggccatc gtctaagcca aaacgcatac ggaggatttt ttgttcacgt ggagagaggt
     7141 attgtaagaa ttccgatacg tgatcgcgaa gcaactggta gatagcttgt tcttcgggac
     7201 catgttcggt tgacggaatg aagtctccaa gagctgaatc ttcctcttct cctacaggag
     7261 cttcgagaga gaccgtttcc tgagaaatct tcataatgtg ttcaacctta tcaaccggaa
     7321 ttttcattgc cttagcaatt tcttctggcg atggttcacg gccaagctct tgcactaagc
     7381 gacgttgctc acggataaga cggttaatag tttctaccat gtgaacagga atacgaattg
     7441 tacgagcttg gtcggcaata gcacgggtaa tagcctggcg aatccaccag gtggcgtaag
     7501 ttgagaactt gaatccttta ttgaagtcga acttttcgac agcacgcatg agacccatgt
     7561 taccctcttg gatcaaatcc agaaggctta aaccacggcc aatgtatttc ttcgcaatcg
     7621 aaacaaccaa tcgtaagtta gcctgagcga ggcgcatctt ggcgttggtg tcgcccttca
     7681 taatgcgttt cgccaaagac acttcctcat cgcccgacaa gagaggataa tggccgattt
     7741 ctcgaagata catacggact gagtcgtcgg agatatcacc gagttcatcg tctttgacat
     7801 cttttttgtt tggttccgaa ttaggcaatt gaaggaggga tttcgattcg cgaacttcaa
     7861 taccacggga tgctaacagc tcataaaggc ggtcaagcaa aataatatcg tcttcgatag
     7921 tgggaaacga ctgtaagatt tcctggtagg tcacaaaacc aaattgacga cctttgtcga
     7981 ctaaacgttt taatgactct ggaaaatcag ctggaatttc atctacc
//
LOCUS       DASWYB010000081         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_302155, whole genome shotgun sequence.
ACCESSION   DASWYB010000081 DASWYB010000000
VERSION     DASWYB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302155"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VGE59_04690"
     CDS             <1..957
                     /locus_tag="VGE59_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074954.1"
                     /translation="AYARANRLSSPLTEKSIEWIHRICRKDTDQSIRLLPKLFYECLN
                     HKTDNTHNSFFVTLGQASLFGWIAYTIYDDFLDGEGDKTALPVANVALRETVNLLRQA
                     LPDNPEFHRHVSQILDQLEAANAWETTHCRTSPLLSLEEIGGKLPNYQDLSQLAERSL
                     GHALGVLGVLYHADYTRKRPEIKQTETFFRHYIIARQLNDDAHDWQDDLKAGHLTAVT
                     TQLLRQALVTLPIKNHPRSILKLLPKLEDLFWQEELLRICNLISYHLSRARTALKNNP
                     AIHYPDPLLALLHPIEASITRARETHHTTGEFLDNYSPPSSI"
     gene            972..1802
                     /locus_tag="VGE59_04695"
     CDS             972..1802
                     /locus_tag="VGE59_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1074955.1"
                     /translation="MRDILSLANMALFPARAAMCQPDLSKGNLPVTYEEAVEELVKAR
                     LLLQAQAPFIHLASITSGIINQINNLLSRPTMQCSLILEAPDLPEEVKRRIGCMKGDL
                     AVLAQLVRLINADFHGGPEDNTRRSANEMVNLADLLEYVRALAAHRAEFKQHMVDIAV
                     DASPARIVLPSPLLRIALINLAFNGVDATPVGSVTLKGDILPDYVEIHVTDTGHGMSP
                     EILTQCRESFFTTRLSEGGCGLGLWTADQIIQSVNGTLEIHSEQGSGTCCLIRLPLIT
                     "
     gene            1974..2480
                     /locus_tag="VGE59_04700"
     CDS             1974..2480
                     /locus_tag="VGE59_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEY1074956.1"
                     /translation="MSTDTPEPMDMATKPIKEHEWLQKLVGEWRVESEMVMPEGTKLK
                     TQGTARTKSLGGLWAFTESTDTMPDGTKMESYFALGYDVSFKEYRGCMVMSASSHLWK
                     YTGELSADGKTMTFNCEGPSMITDGETALYRDVIELIDENHRTLTSNGQDEKGEWQEF
                     MKAHYYRV"
     gene            2643..2834
                     /locus_tag="VGE59_04705"
     CDS             2643..2834
                     /locus_tag="VGE59_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074957.1"
                     /translation="MNHFTKEELEEALRAIASTISKCEKVQPKLKPGTSQHTLLIRRI
                     KALTIASALITQALDSQEK"
     gene            complement(2889..>3375)
                     /locus_tag="VGE59_04710"
     CDS             complement(2889..>3375)
                     /locus_tag="VGE59_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1074958.1"
                     /translation="VAESIPWDDGTFDSVVMADVLEHCANDRDSLQEVFRILKENGRA
                     VITVPAYAWLWSGHDTANHHYRRYSKQELQQKLLASGFVIEKITYYNTFLFPFVVLTR
                     GLKKVLRRTGSDLTMPPAIINKFLAAVFDSEKVILSRGGVFPFGVSLLCVVRRPMTNK
                     V"
BASE COUNT          995 a          873 c          687 g          820 t
ORIGIN      
        1 gcctatgcgc gtgcgaatcg cctttcttca cccctcacag aaaaaagcat tgaatggatt
       61 catcgcattt gtcgcaaaga tactgatcag tcgattcgtc ttttaccaaa actattttat
      121 gaatgtttaa accataaaac agataacacg cacaactctt ttttcgttac attgggccaa
      181 gcttctttat ttggctggat tgcctacact atatacgatg acttcctcga cggagaaggg
      241 gacaaaactg ccctacctgt ggcaaatgta gctttacgcg aaacggtcaa cctattacgg
      301 caagccctac cagataaccc cgagttccac cgacacgttt ctcagattct tgaccaatta
      361 gaggcggcca atgcgtggga aacaactcac tgccgtacat ctccgctcct ttctttggaa
      421 gagattgggg gaaagctacc caactaccaa gacctttctc agcttgccga acgttcactc
      481 ggacatgctc tcggagtgct cggcgtactc tatcacgcag actacacaag aaaaagaccc
      541 gaaataaaac aaactgaaac cttttttcgt cactatatta ttgcgcgcca acttaacgac
      601 gatgcccatg actggcaaga cgatctaaag gctggccacc tcacggccgt aacgacacaa
      661 cttttacgcc aggctctcgt aacgctacca ataaaaaacc accctaggtc tatactgaaa
      721 cttttgccca aacttgaaga tttattttgg caggaggagc tcctccgtat ctgcaacctt
      781 atttcctacc acctctccag agcacgaact gctctcaaaa ataaccctgc catacactat
      841 cccgatccac ttctcgctct ccttcacccc attgaagcat caataactcg agcaagagag
      901 acgcaccata ccacgggcga atttctggat aactactctc caccttcgtc tatttaatat
      961 ttttttaagg catgagagat atacttagtt tagccaatat ggctcttttc cctgcgagag
     1021 ctgccatgtg ccaaccagat ttgtccaagg gtaatcttcc cgtcacgtat gaagaagcag
     1081 ttgaagagct cgtaaaggca cggttgctac ttcaagctca ggcacctttt atccaccttg
     1141 catcaatcac ctctgggatt ataaaccaga tcaataacct gctcagccgt ccaacgatgc
     1201 agtgctctct catcctcgaa gccccagatt tacccgagga agtcaagaga cggatcggat
     1261 gtatgaaagg agacttggca gttcttgccc agttagttcg acttattaat gccgactttc
     1321 atgggggacc agaggataat accaggaggt cggccaatga aatggttaat ctcgctgatc
     1381 ttctggaata tgttcgcgca cttgcagcac atcgtgccga gttcaagcaa catatggtcg
     1441 atatagcggt agatgcgtca cccgcacgta tcgttctccc ctctccgctc ctgcgtatcg
     1501 ccttgatcaa cttggcgttc aacggtgtgg acgccactcc ggttggtagc gtaaccctca
     1561 aaggagacat cctccctgac tacgtagaaa tacacgtcac ggatactgga cacggtatgt
     1621 ctcccgagat ccttacccag tgtcgagaat ctttctttac cactcgatta tcagaaggag
     1681 ggtgcggcct cggtctgtgg actgccgatc agatcatcca atccgtcaac ggcacactcg
     1741 aaatccacag tgaacagggt tccggaacct gctgcctcat ccggctccca cttatcacct
     1801 agcccacctc tctcgcgtat ccttcccttg cgcgtctcca cccggagacg cgttttttcg
     1861 tcaaatttaa agaagtgcat atgaaaaaac tttttcacct aggcacaata gatattattt
     1921 tataaattat gctacagtca tagtaactat cctccaaaca aaaggaccac actatgagca
     1981 ccgacacacc tgagccaatg gatatggcaa ccaaaccgat aaaagaacac gagtggctcc
     2041 aaaagctcgt gggagagtgg agagttgaaa gtgagatggt catgccagag ggcacaaaac
     2101 tcaagactca gggaaccgca cgcactaaaa gcctcggagg gttatgggca tttaccgaaa
     2161 gtacagatac tatgcctgat gggaccaaaa tggaatcata ctttgcgctc ggctatgatg
     2221 tctctttcaa agaataccga ggctgtatgg taatgtcggc atcatcccat ctttggaaat
     2281 atacgggaga actcagtgcg gatggaaaaa cgatgacatt taactgcgaa ggcccaagca
     2341 tgattacaga cggcgagaca gccctttacc gcgacgttat cgaactcatc gatgaaaacc
     2401 accgtacact cacctcaaat ggccaagatg aaaaaggcga gtggcaagaa tttatgaagg
     2461 cgcattacta ccgagtataa actcaggcca gtatacgcaa ttactacata cggaaaagaa
     2521 gaggtttctc acctcttctt ttcaaattga ataatagtgc gacaagggcg taaagcgtgt
     2581 tttcaagagg aacggtgtat aataactatg agaaaattaa ctctacagag gaaatagtag
     2641 gtatgaatca ttttaccaaa gaagaattag aagaagctct ccgagcaatc gcctcaacaa
     2701 ttagcaagtg tgagaaagta cagcccaaac taaaacccgg tacttctcaa cacacattac
     2761 tcatacgcag aattaaagcg cttactattg cctcagcttt aataactcag gcactcgata
     2821 gccaagaaaa ataatacagc cacctccagg attgggccat cagaattaat cccctcggtc
     2881 tgatctattt aaaccttatt cgtcatggga cgtctcacca cacacaaaag agagacaccg
     2941 aacgggaata caccaccccg tgatagtatt actttttctg aatcaaaaac tgcagctagg
     3001 aacttattta taatcgctgg cggcatggtc aaatcactac cagttcgacg caacactttt
     3061 ttgagacctc gagtcaaaac aacgaacgga aaaagaaacg tattataata ggtgattttt
     3121 tcgataacga aacccgaagc tagaagtttt tgctgtaact cttgtttgct gtaacgtcga
     3181 tagtgatgat ttgcagtatc gtgtccactc cacaaccaag cgtacgccgg tacagtaatg
     3241 actgcccgac cattttcttt aagaatccgg aaaacttcct gcaggctgtc acgatcatta
     3301 gcacagtgct cgagcacgtc ggccatcaca actgaatcaa aggttccgtc atcccagggg
     3361 atagattccg ccaca
//
LOCUS       DASWYB010000082         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_504623, whole genome shotgun sequence.
ACCESSION   DASWYB010000082 DASWYB010000000
VERSION     DASWYB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504623"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1121
                     /gene="ruvB"
                     /locus_tag="VGE59_04715"
     CDS             105..1121
                     /gene="ruvB"
                     /locus_tag="VGE59_04715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY1074959.1"
                     /translation="MAISTYDDESDVPAGVEESGSPTPSLDTALRPRILDDYVGQEAI
                     KKSLTLTLQAAKQRDEPAEHILFAGPPGLGKTTLAGIIASELQVPFRITSGPALERSG
                     DLASIIASLQPGEVLFIDEIHRLNRNIEEMLYPVMEDYALDLVLGKGAGARTMRIQLP
                     RFTLVGATTRPGSLSSPLRDRFGLHYHLEYYTERELTHIITRTASLLDVDIEDAAAHA
                     LAIRSRGTPRVANRLVKRVRDYATVHGDGLIRLRDVQEALHDLGIDDKGLDAIDRRIL
                     KTIIEQFDGGPVGVETIAAATAIERATLEDVYEPYLLQIGFLQRTPRGRKVTDLAIAY
                     LNSD"
     gene            1219..2481
                     /locus_tag="VGE59_04720"
     CDS             1219..2481
                     /locus_tag="VGE59_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1074960.1"
                     /translation="MNSVSERATRHQLPIVILTLILDYIGFAILIPIGPLLFTEPSSP
                     HYLLSNYSSDSGYILFGLLVASYPLAQFLAAPVLGQLSDRYGRKPVLSIALVGTMFSY
                     ILFALGIFTANIPLLFFSRILDGITGANVTVAQASLADITEPQHRSKVFGYMGASIGV
                     GITLGPLVGGKLSDPSVVNWFNSATPFWFAAILSFLNVLSVQAFFVETLKTKNNQATL
                     KINKSFANIVRAFSLPNLRTLFTTSFLFGAGFAFYTAFASVFLVHRFGFDQSKLGNFF
                     FYTGMMVVLTQAFVPRLMATRFSERQLLRVSLIAASVFPLLYVLTHVWWCLLLIFPFY
                     SAFVSLSMANITSLVSKSAKAENQGEVLGINTSVQGLAQSIPPILGGIVAAQWVYFAP
                     LVVSAGTLFLAGVVFLLGYKSEGKPNES"
     gene            2504..3121
                     /locus_tag="VGE59_04725"
     CDS             2504..3121
                     /locus_tag="VGE59_04725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1074961.1"
                     /translation="MLHIYLARHGQDQDNIQGILNGRRDMPLTEKGLEQAGLVAGELQ
                     ASGIHFDKIYASPLQRAYKTAEIIADAVGAEKPEPMELLIERDFGIMTGKPQSEIVAL
                     CSPDIIKTSTITYFLSPEGAETFPDLIVRAKEILEEIRGKHTDGNILLVSHGDIGKMI
                     YCAFYNLDWQDVLHMFHFGNSEVLVLSPDSNAQDVYLYQAEQYNL"
BASE COUNT          846 a          765 c          800 g          973 t
ORIGIN      
        1 gcagtataat ggctatatgc ccggtggtta tgggtactac cgcgtaatag accacggtaa
       61 tggcctctac accatgtacg gccacctcac gaactaaccg acccatggca atttctacct
      121 acgacgatga atcagatgta ccagcaggcg tagaagaatc cgggtcaccg actccttctc
      181 tggataccgc gctacgccct cggatcctgg acgattacgt cggtcaagag gcaattaaaa
      241 aaagtctcac tcttacgtta caagcagcga aacaacggga tgaaccggca gagcatattc
      301 tctttgccgg acctcccggt ttaggaaaga ctaccctggc gggaattatt gcctccgaac
      361 ttcaagttcc gtttcgtatt acctctggtc cggctctaga acgaagtggc gatctggctt
      421 cgattatagc gagcttgcaa ccaggcgagg tactctttat cgatgagatt caccgcctta
      481 accgaaatat tgaagagatg ctctacccgg tgatggaaga ctatgcccta gacttagtcc
      541 tgggcaaagg ggcaggtgct cgcaccatgc gcattcagtt gcctcgtttt acgctcgtgg
      601 gggcgacaac ccggccaggc agtctgtcgt ctcctttacg ggatcgcttt gggttgcact
      661 accaccttga atattacacg gagcgagagc taacgcatat tattacgcgg acggcgagtt
      721 tactggatgt ggacatagaa gacgctgctg cccatgcctt agccattcgg tctcgcggta
      781 cgccccgagt tgccaatcga ctcgtaaaac gcgtgagaga ctacgctacg gtccatggcg
      841 atggccttat tcgtttgcgg gacgtgcaag aagccttgca cgatttaggg atagacgata
      901 aaggtctcga cgcaattgat cgccgtattc taaaaactat tattgagcag ttcgatggtg
      961 gtcctgttgg tgtagaaaca attgcagctg ccactgccat cgaacgggcc acattagaag
     1021 atgtctacga accatattta ttacaaatcg gatttctcca acgcacccca cgtggaagaa
     1081 aagtaacgga tctcgctata gcctatctaa actcagatta aatatgatga atgtttcgag
     1141 aaaaagaaag tagattcgtt aatagtctga aaccaattaa gcagctttat ggctgttttt
     1201 ttggtatcat atacccatat gaattctgtt tctgaaagag caacacgtca tcagcttccg
     1261 attgttattc tgacattaat attagattac attggttttg ctattttaat tccgattggt
     1321 ccacttctct ttacggaacc tagctcgcct cattatctac tgtcaaacta ctcgtcggat
     1381 tcaggctata ttctttttgg acttttagtg gcctcgtatc ctttggctca gtttttagca
     1441 gcacctgtgc ttgggcagtt atcagatcgg tatgggagaa aacctgtact tagtattgcg
     1501 cttgtaggaa ccatgttctc ctatatttta tttgcactcg gtattttcac tgctaatatc
     1561 ccacttttat ttttctctcg tattctcgat ggtattaccg gtgcaaatgt gaccgtcgca
     1621 caagcctcct tagctgatat caccgagcct caacatcgtt caaaagtatt tggatatatg
     1681 ggagcctcaa tcggagttgg tattacgtta gggccactgg tcggtggcaa gttgtctgat
     1741 ccgtcagttg tgaactggtt taattcagcc acaccgtttt ggtttgctgc tatcttaagt
     1801 tttcttaatg ttctttctgt tcaagccttc tttgtagaaa ccctcaaaac gaagaacaat
     1861 caagccacac tcaagatcaa taagtcgttt gctaatattg ttcgagcatt ttcgctgcca
     1921 aatttacgaa ctctttttac aacaagcttt ttatttggcg ccggttttgc cttttatact
     1981 gcatttgctt ctgttttcct ggttcatcgc tttggatttg atcaatcaaa gctcggtaat
     2041 ttctttttct acacgggaat gatggtggtg ttgacgcaag cctttgtacc gcgcttaatg
     2101 gcaactcgat ttagtgaaag gcaactgctt cgtgtaagtt tgattgcggc atccgttttc
     2161 cctcttctgt acgtgctcac ccatgtttgg tggtgcttgt tgcttatttt tccgttttat
     2221 tccgccttcg tatccctctc gatggctaat atcacctcgc tcgtgagtaa gtctgccaag
     2281 gccgaaaacc agggtgaagt actaggaatt aatacaagcg tgcaaggtct cgcgcagtcg
     2341 attccgccaa tcttaggcgg gattgttgcg gcccagtggg tttactttgc tcctttggtt
     2401 gtctcagcgg gaacgctctt tttagcggga gtggtgtttc ttctggggta taagtcagaa
     2461 ggaaagccga acgaatcgta aaaatatttg ctatactcag aacatgttac atatttacct
     2521 cgctcgacac ggtcaagatc aagataatat tcaagggata cttaacggac ggcgtgatat
     2581 gccgttgaca gagaaaggtt tagaacaagc tggcttggta gcgggggagc tacaggcttc
     2641 ggggattcat tttgataaaa tatacgcttc gcctcttcag cgagcttata agacggctga
     2701 gattattgct gacgcggttg gggctgaaaa acccgaacct atggagttac tcattgagcg
     2761 tgactttgga attatgaccg gaaagccaca aagtgaaatt gttgccctgt gttcacccga
     2821 tataattaag accagcacaa ttacctactt cttatctccc gaaggagcgg agacgtttcc
     2881 ggatctcatt gtccgcgcaa aggagattct ggaagaaatc agaggtaaac atacggatgg
     2941 aaatatttta ttggtcagcc atggagatat tggcaaaatg atatattgtg ccttttataa
     3001 cctggactgg caggacgtgc tgcacatgtt tcattttggg aattccgagg tacttgttct
     3061 ctcgcctgat tctaatgctc aagatgtcta tctctatcag gcagaacagt ataaccttta
     3121 gttaggatta tataaatata aaggaaagag agccgacgat accgcacggg taacaaccgg
     3181 ctctcttgac acgttctggc gaggaattag cgctgagtcg ccttcctgag gatgaaaatg
     3241 aggaaggcca cgcctactgc gcttggccag aaatggatgc tgggccagat cagcccgagt
     3301 gcgaaggccc cgaccagagg gagtgccacg taatagaaga ggaagtggag caccctcagt
     3361 atctggtaga tgatttgatc agcg
//
LOCUS       DASWYB010000083        10303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_97024, whole genome shotgun sequence.
ACCESSION   DASWYB010000083 DASWYB010000000
VERSION     DASWYB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97024"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VGE59_04730"
     CDS             <1..1428
                     /locus_tag="VGE59_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074962.1"
                     /translation="HTSFLMNGLKVVGETVTKSRPAKQVASFETGSLEPEETSVVTEE
                     STKIKSPVEITSPVHNSTEEGDFRVHTYWQGKTSVSPHVEEIEEAETELYQTRPPLVT
                     SQVNIWKRTQPRTLYLLLGCLAVLGLTIKVVNTVLKHTPDTSQAIAERDSLITQAQES
                     ARKAETALVSDDKTTAIAEFLASQDFLAQISDKNQTETSRSLAARTSEKIDTLTKTTQ
                     LSQTQSAQSLPTASKQVTTTANGTYILTHENTLYKYTATELTPLTTLPTGFALTEAVP
                     YENLQKLALYGKTSDTVPALLSIVTVTDQLTPLTRSDSAPWPDAKHIASFESNLYLVG
                     DTMSKAIPKDSAYRVIPYANNDSTKTITNILNNGFAFYALEEGKQLVRIAANTAKTPV
                     KLYGVPDAFLPTAINRLISTRKEGSLYLLDTNGQRILEISTDGAYRQQYKLPKNDTYT
                     DCDATDKEFICTTTKKEIKTIPFSS"
     gene            complement(1458..3158)
                     /gene="argS"
                     /locus_tag="VGE59_04735"
     CDS             complement(1458..3158)
                     /gene="argS"
                     /locus_tag="VGE59_04735"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY1074963.1"
                     /translation="MMQQLRDALKTATRTAYPHIALPTYEVSRPDTSAHGDFSTNLAL
                     IFARMVKENPYDVAEKIVAHLPKLEMVEKVETVRPGFINFYMKPACFLDKLTELVEQG
                     ITITPQSTEPERILLEFGQPNTHKLPHIGHLFSYIYGASCANLLEATGVNVLRLNYQG
                     DVGPHVAKCLWAYRKKQQADPELLEDKISYLQQCYQEGAQAYEEDTTAKAEINALNTE
                     IYEHDPDVMSDWKKTREWSVAYYRDHLHKVLGISYDTLYYESETGPLGKSLVKEKVGL
                     VFEEDQGAVVFRGEQFGLHTRVFLSSAGNPTYEAKEIGLFACKKRDYYPFDRAVITTA
                     VEQNEYWQVVKKAAEMVFPDLEGKIKHIGYGMINLTSGKMSSRTGNILSAFGLIDLVR
                     DTVKEKVAARDTYTQEEISTITHEVTLGAIRYSFLKTSPGKNIAFDLDTSIAFEGNSG
                     PYLQYTYARCQSVLRKAEVEASVSDAIDPNDEELAVLRWLSRYDEVVHAAAESYAPHL
                     VANYLFELAQKYSYFYNQHHILTEDQGQTQFRLAITKVVGETLKHGLHLLGIETLEKI
                     "
     gene            complement(3155..4375)
                     /gene="tyrS"
                     /locus_tag="VGE59_04740"
     CDS             complement(3155..4375)
                     /gene="tyrS"
                     /locus_tag="VGE59_04740"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY1074964.1"
                     /translation="MDTHSYSHSSLLTRRVIEVIDRDHIAKRLTAGEKLRVKLGIDTN
                     KPDLHIGHAIPLWKLREFQDAGHTAVLILGDYTSQLGDPTDRSEARKTISEEETKANA
                     AKCLDQVFKILDSDKTEIRYNSEWFDTFNLRDTINLMGATTLNQLLAHETFANRLSGG
                     TPLYTHEILYPLLQGYDSVMVEADLELGGLDQKFNVLMGRTIQRAHNKPEQDVMLFAY
                     LPGTDGQAKMSKSLGNTVNLSDTPNDMFGKVMSIPDDLLASYFELATSLPEKDIEVTL
                     ARLSSGELHPRDAKVILAKQIVSQYHNQDAADKAEEHFNKVFRDKQTPEDIETLILPQ
                     GTYDLADILVTRSTLISSKSEFRRLVQQRGIKVNQKPVEDLAATIATEQGDDIIIQVG
                     ARRFIKVNWKVGAK"
     gene            4482..7319
                     /locus_tag="VGE59_04745"
     CDS             4482..7319
                     /locus_tag="VGE59_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY1074965.1"
                     /translation="MFKKFLKKVKRSAKKIPSVPDVKQIQSNLTIKKLRKKLTWRFIF
                     RWAIALAIAGLVCGALLFAWYFKDLPRPGELRNRNAEESTILYDRNGKQIYDISGDER
                     RILLKSSEIPDVIKEATIAIEDKNFYHHYGFDFKGLARAALGLGTQGGGSTITQQYIK
                     NAILSPQFSLTRKIKELILAIELEAIYSKDDILTLYLNEIPYGSNIYGVEAASQAYFG
                     KSAKDGLTLSEAATLAAIPQAPTRYSPYGNNLNLLMARKNKVLDDMVKTNAITSEEAD
                     KAKTEAPLVAKDFAQKRDNFPAPHFVMYIREQLVKKYGEEMVQRGGLRVTTTLDLDLQ
                     EKADDAVKNQGAKTLPRVKASNASLVAIDPRSGHLLAMVGSLDYFNRDNEGNFNVATA
                     QRQPGSSIKPLVYATLFKDKYSPGSVFWDVPTDFNGYKPNNFDGRFRGPLTLRSALGS
                     SLNIPAVKALSLAGVNDFIETATDLGITTYGDKKGAGLALALGAGETKLVDLTAAYSV
                     FANNGDKHPLTSIIKIQDSRGKVLDEWKDSSKQVLKPEIAYEVSEMLADVNAKRATFG
                     SAVNLLSVSGHAVASKTGTTNDFKDALTVGYTPSISVGVWAGNNNGDEMEHGGGSTAA
                     APIWHDFMLKVLSGKPNEPFSRPNTISEITVDMLSGKKPTPASGQLIKDLFAPWQLPT
                     KDDDVHQVIKVCKSNGLLATDATPAEEIEEKVFTKVHSERPDNPAWEIPVQSWAKASN
                     LVSDPPTEKCDIAFTNPKVDITSPTENATVSGIFTATADVIFPPNNDYGTVEFSVDGK
                     SVSKDTAEPFTATIDASSLSSGSHTILVTATGSNGAQGTDSITISVSKDTTAPGDVTN
                     VTLLPGAKNATVSWTNPSDSDLSSVTFYVSQSSGVKGSKVGTVNANQSTTQTTVLNNL
                     VSGITNYVTIITTDKSGNESSSTKQYPVTPL"
     gene            complement(7376..7897)
                     /locus_tag="VGE59_04750"
     CDS             complement(7376..7897)
                     /locus_tag="VGE59_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1074966.1"
                     /translation="MPTSKISLDHAKPDKLFYFVANVVVYRKSDGRCLLLKRSEREKV
                     HPGKYAIPGGKLEWANLDSTKATRLNGDVIDFDNAVEDLIAREVFEEAGITVSSQLTY
                     LRSVAFIRPDGVPVILVKFAAMYESGDVKLEEGAFSDFVWVNSAEVVSYACIDGIHDE
                     VKRTISLFTSNNE"
     gene            complement(7878..8822)
                     /locus_tag="VGE59_04755"
     CDS             complement(7878..8822)
                     /locus_tag="VGE59_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY1074967.1"
                     /translation="MIESQQDLLFLSIALAVAVCAYFIFRRLLHVSFPYFFLGLVGLI
                     TGLIIGSLVSGLLDGLPGNFGRWLPIIVNVIIAVGIFDLFLAQAQSATDFLRVILKRD
                     EKSNSFDVVLDTSVLIDGRIEQIVETGFIPGTLIVPQVVLQELQKLADARDPMKRAKG
                     KRGLNILASLRNQPRVKITIVQEPKAEETVDAELIRISQKHNAHLLTLDYNLNQIAHI
                     RGVRVLNINALASALRPVLLPGESLMVAIIQKGKEPGQGLGYLPDGTMIIVEGGDTLV
                     DHEVECEVVRIYQTVSGKIVFVAPKGSKKEKSRANLKD"
     gene            complement(8815..10164)
                     /gene="radA"
                     /locus_tag="VGE59_04760"
     CDS             complement(8815..10164)
                     /gene="radA"
                     /locus_tag="VGE59_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY1074968.1"
                     /translation="MAKTRTMYVCDNCGEESIAWQGRCPVCQEWNTFKEFKTEVRGSG
                     GSGRSNTSGQALDLKPLNTQIETGIKSRIETRLPEFDRVLGGGIVPGSVVLLGGEPGI
                     GKSTLLLQVAQYVADSHTVIYFSGEESESQVAGRAKRLGLNPTFLFANETRLGTIQQT
                     LTQLKPTLVIIDSIQTLYDDAFPSTPGSLVQVRECSLRLQEFAKANGISIILVGHITK
                     EGTVAGPKTLEHMVDVVLYLEGEPRSEMRILRSMKNRFGATYEVGLFELGEKGMSSIA
                     DPSSLFIEDHTGEVPGTALTAVLEGVRPILVEVQALVVPTAFGYPKRTSSGVDLQRLH
                     ILLAILESRTGASFSSADVFLNVVGGYRISDRAADLAICMALLSAASGVALPAKQVFY
                     GEVGLTGEVRRVGQMERRVTEVTRLGYLASPAFKSVRDIGNEFGVLKMRRRKANTDD"
BASE COUNT         2948 a         2607 c         2095 g         2653 t
ORIGIN      
        1 cacacgtcat ttttaatgaa tgggctcaaa gttgtgggcg aaaccgtgac gaaatcccgc
       61 cccgcaaagc aagtcgcctc ttttgaaact ggatccttag aacccgagga aacatccgtc
      121 gtcaccgaag aatcaaccaa gataaagtcc cctgtagaaa tcaccagtcc cgtgcacaac
      181 agtacggaag aaggcgattt tcgtgtccat acctattggc aaggaaaaac atccgtatct
      241 cctcacgttg aagaaatcga agaggcagag acagagcttt accaaacacg cccaccgctt
      301 gtaacaagcc aggtgaacat ttggaaacgg acgcaacccc gaaccctcta tcttctgctt
      361 ggctgccttg cagtacttgg acttacgata aaagtagtta acaccgttct taaacatacc
      421 ccagatacct cacaagccat cgctgagaga gactctctca ttacacaagc gcaagagtca
      481 gcgcgcaaag cagaaaccgc tttagtgagc gatgacaaaa caacagcgat tgccgagttc
      541 cttgccagcc aagatttttt ggcccagatc agtgacaaga accagaccga gacaagccgc
      601 agccttgcag cacgtacgag cgaaaaaatc gacacgctta caaaaacgac ccaactctca
      661 caaacacaat cagcccaaag tctcccgact gcaagcaaac aagtgacaac aaccgcaaac
      721 ggcacttaca tccttaccca cgagaacaca ctttataaat ataccgcgac cgaacttaca
      781 cctctcacaa ctcttccgac aggattcgca ctgactgaag ccgtacccta tgaaaacttg
      841 caaaaactcg ccctttacgg aaaaaccagc gatacggttc ccgctcttct gtctattgtc
      901 accgtcaccg accaactgac gcccctcact cgtagtgatt ctgctccttg gccagacgcc
      961 aaacacattg ccagttttga atcgaacctt tacctagttg gcgataccat gagcaaagca
     1021 attcctaagg actcggcata tcgagtcatt ccttatgcca acaacgactc taccaaaaca
     1081 atcaccaata ttttaaataa cggctttgca ttttatgctc tcgaagaagg aaaacaactc
     1141 gtacgcattg ctgcaaacac cgccaaaact cctgtgaaac tctacggtgt tcccgatgcg
     1201 tttttaccta ctgctattaa ccgtttaatt tccacacgca aagaaggctc tctttacctc
     1261 cttgatacaa acgggcaacg cattttagaa atcagcaccg acggcgcata tcgtcagcag
     1321 tacaaacttc ccaaaaatga cacctacact gattgtgatg caaccgataa agaatttatc
     1381 tgcaccacaa ctaaaaaaga aataaagaca attcctttta gctcgtaaaa agcagctacc
     1441 tactagctgc tttttagtta gatcttttcg agagtttcaa taccgagaag gtggagaccg
     1501 tgtttaagcg tttctcctac cacttttgta atggctaaac ggaattgagt ttgcccttgg
     1561 tcctctgtca aaatgtgatg ctgattatag aaataactgt atttctgagc cagctcaaat
     1621 aagtaatttg cgacaagatg tggggcatag ctctcagcag cagcgtgtac aacttcatca
     1681 tatcgagaaa gccaacgtag gacagctaac tcttcgtcat taggatcaat cgcgtcactt
     1741 acacttgctt ctacctctgc cttgcgcaat acactctgac aacgagcata ggtgtattgt
     1801 aagtaaggcc ccgagtttcc ttcaaatgca atactagtat ccaaatcaaa cgcgatattt
     1861 ttaccaggag acgtttttaa gaacgaatag cgaatcgccc ctaaagttac ttcgtgcgtt
     1921 attgttgaaa tctcttcttg tgtataggta tcccgagcag ccaccttttc ttttaccgta
     1981 tcacgaacca aatcaatcaa tccaaaagcc gaaagaatgt ttcctgttcg ggaagacatt
     2041 ttgccactcg taagattaat catgccatac ccgatatgtt tgatcttgcc ctctaaatca
     2101 ggaaacacca tttcagccgc ttttttaacg acctgccagt attcattttg ttcaacagca
     2161 gtcgtgataa cggcacgatc aaaaggatag taatcccgtt ttttacaggc gaacaatcca
     2221 atctctttag cctcatacgt gggattaccg gcgctactca aaaatactcg agtatgcaat
     2281 ccaaactgtt ctccacgaaa aacgactgcc ccttgatcct cttcgaatac aaggccaact
     2341 ttttctttga cgagagattt cccgagcgga cctgtttcac tttcgtagta cagcgtatcg
     2401 taagaaatac cgagcacttt gtgcaggtgg tctctatagt acgcaacact ccattctcga
     2461 gtttttttcc aatcagacat gacgtctgga tcatgctcgt aaatttccgt atttaaagca
     2521 ttaatttccg ctttcgcggt tgtatcttcc tcatatgcct gtgccccttc ttggtagcat
     2581 tgctggagat agcttatttt gtcctcgagt aattctgggt cggcttgttg cttcttacga
     2641 taggcccata gacattttgc cacatgtggc cctacatctc cctggtagtt caaacgaaga
     2701 acgttcacgc cagtcgcctc taataagttc gcacaacttg caccatagat gtaagagaac
     2761 agatgaccaa tatgcggaag cttatgcgta ttgggctggc cgaattctaa taaaatacgc
     2821 tcaggctcag tactctgagg agtaatagtt attccttgct caactaactc tgttaattta
     2881 tcaaggaaac acgctggctt catataaaag ttaataaacc cagggcgaac cgtttctact
     2941 ttctctacca tttctaactt aggaagatga gccacaatct tctcagcaac atcatacgga
     3001 ttctctttga ccatccgggc gaaaataagg gctaagttcg tcgaaaaatc accgtgtgcc
     3061 gaagtatccg gtcgactaac ttcataggta ggaagcgcaa tgtgcgggta cgcggttcgg
     3121 gttgccgttt tgagtgcgtc acgaagctgc tgcatcattt ggcgccaact ttccagttca
     3181 ctttaataaa gcgacgagcc ccgacttgaa taataatatc atctccctgc tcggtggcga
     3241 ttgtggcggc gagatcttca accggttttt gattaacttt aatccctcgt tgttgcacca
     3301 atcggcgaaa ctcactttta cttgaaatca atgttgaacg tgtcactaaa atgtccgcga
     3361 ggtcataagt accctgtggc aaaataagag tctcgatatc ttcgggagtc tgtttatcgc
     3421 ggaacacttt attaaaatgc tcctcagctt tgtccgcggc atcttggttg tggtattgcg
     3481 atacaatttg tttcgctaaa attacttttg catctcgtgg gtgtaattca cccgagctaa
     3541 gccgcgcaag ggttacttca atatccttct cgggcagtga cgttgcaagt tcgaagtatg
     3601 aagcaagtaa atcatcagga atagacatta ctttaccgaa catgtcgttg ggagtgtcgg
     3661 ataaattaac cgtgttacct aaactcttcg acatcttggc ctggccatca gtaccaggaa
     3721 gatatgcaaa gagcatcaca tcttgctccg gcttattgtg cgctctttgt atagtgcgcc
     3781 ccatcaagac attaaacttt tgatccaatc caccgagctc aagatctgcc tctaccataa
     3841 ccgagtcata tccttggagt aagggataga gtatttcatg ggtatacaga ggagtgccac
     3901 cgctcaaacg gttggcaaat gtttcgtgag ccaaaagttg gttgagagta gtagctccca
     3961 tgaggttgat tgtgtcgcgt aagttaaagg tatcaaacca ctcagagtta tagcgaattt
     4021 ctgttttatc cgaatccaat attttaaaaa cctggtctaa acatttagcc gcgttcgctt
     4081 tggtctcttc ttcagaaatt gtcttacgtg cctcgctccg gtcggtggga tctccgagct
     4141 gtgacgtgta atcgcccaga attaaaaccg cagtatggcc tgcgtcttga aattcgcgta
     4201 gcttccataa aggaatcgca tgtccaatat gtaaatcggg tttattggta tcaatgccaa
     4261 gtttaacgcg taacttttct ccggcggtta aacgcttggc aatgtgatct cggtcgataa
     4321 cttcaataac ccgtcgagtg agaagtgaag agtgagaata agagtgtgta tccattaccg
     4381 tataattata ctctgaagga cgggacttcg tctacggagc caacagacca ctattcttgc
     4441 tatcggcgct cggagtaagt acaatagaag taataaaaat tatgttcaaa aaatttctta
     4501 aaaaagttaa gcgctctgcc aaaaagatcc cttctgtccc cgacgttaag cagattcaat
     4561 ccaacctaac cattaaaaag ttacgcaaaa agctgacgtg gcgatttatt tttcgctggg
     4621 caattgcttt agcaattgct ggacttgtat gtggcgcgct tctttttgct tggtacttta
     4681 aagatctccc ccgcccgggc gaacttcgta accgtaatgc cgaagaatca accattctct
     4741 acgatcgcaa tggaaagcaa atttacgata tctccggtga tgagcgacga atcttactca
     4801 aatcgagtga gatccctgat gtgataaaag aagccactat tgcaattgaa gataaaaact
     4861 tctatcacca ctatggcttc gactttaaag gcctggcacg cgccgctctc gggctcggca
     4921 cccaaggtgg tggaagtacc attacgcaac aatatattaa aaacgccatt ctttccccac
     4981 aattttcttt gacccgcaaa atcaaagagt tgattttggc cattgagcta gaagcgattt
     5041 actccaaaga cgatatcctg accctttact taaatgaaat tccttacggc tctaatattt
     5101 atggcgtcga agcggcaagc caggcttatt ttgggaagtc ggctaaagat ggtttaacac
     5161 tctccgaagc cgcgacactt gccgccattc cacaggcccc gacacgctat tccccttacg
     5221 gcaacaacct aaaccttctt atggcccgga aaaataaagt cctcgacgac atggtaaaaa
     5281 ccaatgccat tacttccgaa gaagctgata aggcaaaaac tgaagcccca ctcgtcgcaa
     5341 aagattttgc acaaaagcgc gataatttcc ctgcaccgca ctttgttatg tatatccgtg
     5401 aacagctcgt taagaaatac ggtgaagaaa tggttcagcg tggtggtctt cgtgttacta
     5461 ctacacttga cttagacctc caggagaaag ctgacgacgc cgtcaaaaac caaggtgcga
     5521 agacgctgcc acgcgtaaaa gcttctaacg cctccttagt tgcgatagat ccacgcagcg
     5581 gacacctcct cgctatggtt gggtctctcg actatttcaa tcgtgataac gaaggtaact
     5641 ttaatgtcgc cacggctcag cgtcagcctg ggtcttccat taagcccctt gtctatgcca
     5701 cgctttttaa agataagtac agcccgggat cagtattctg ggatgttccc accgacttta
     5761 acggctacaa accaaataac tttgatggcc gcttccgcgg acccctcacc cttcgaagtg
     5821 ctttaggaag ctcactcaac attcctgctg ttaaggctct tagcttggct ggcgtgaacg
     5881 attttattga gacagctacc gacttaggca tcactacgta tggagacaaa aaaggagcgg
     5941 gattagctct cgcactcggt gccggtgaaa caaagctcgt tgatcttacc gctgcgtaca
     6001 gtgtatttgc caataacggc gataagcatc cgttaacttc cattattaaa attcaagata
     6061 gccgcggaaa agtcctcgac gaatggaaag atagcagtaa acaggtcttg aagcccgaaa
     6121 tcgcttacga ggtcagcgaa atgctggctg acgtgaacgc aaaacgagcg acatttggca
     6181 gtgccgtgaa tctgttatca gtgtctggcc acgcagtcgc ttccaaaact ggcaccacca
     6241 atgactttaa agatgctctt accgttggct atacgccttc tatttctgtt ggagtatggg
     6301 ctggaaacaa taatggagat gaaatggagc atggaggggg ttccacagcc gccgctccaa
     6361 tctggcacga cttcatgctc aaagttctct cgggaaaacc taatgagcca ttctctcgtc
     6421 caaatacgat tagcgaaatt acggtcgaca tgttatcggg taaaaaacca acccctgcat
     6481 caggccaact tattaaagac ctctttgccc cctggcaact gcccaccaag gatgacgatg
     6541 tgcatcaggt tataaaagtt tgcaagagca atggtcttct ggcgaccgat gctactccag
     6601 ccgaagaaat agaagaaaag gtgttcacaa aagttcactc tgaacggcca gacaaccccg
     6661 cttgggaaat tccagtacaa agctgggcca aagcaagtaa ccttgtgagt gatccgccaa
     6721 cagaaaaatg cgatatagcc tttaccaatc cgaaagtaga catcacatcc ccgacggaaa
     6781 atgccacagt tagcggaata tttactgcta ccgctgacgt tatattccct cctaataatg
     6841 actatggaac tgtagaattt agtgtcgatg gcaagagcgt gtcaaaagac acagcagaac
     6901 cttttactgc gacgatcgat gcttctagcc tctctagcgg atcacacact attttagtaa
     6961 ccgcaacagg cagtaacggg gcgcagggaa ctgactctat taccatatcg gtttccaaag
     7021 ataccacggc tcctggcgat gtaaccaatg taacgcttct tccgggcgca aagaacgcca
     7081 ccgtgagttg gacaaaccca tcggattctg atttgagctc agtgaccttc tacgtatccc
     7141 aatcatcggg agtaaagggg tcaaaagttg gaacggttaa tgccaatcaa agtaccacac
     7201 aaacgactgt attaaacaac ttagtaagcg gcataacaaa ctacgtaacg atcatcacaa
     7261 ccgacaaaag cggaaatgaa agtagctcta ctaagcaata tcccgtcact ccgctgtagt
     7321 gataaagaaa cgcttcagtc attccaagaa tcttacttta atgatttatc tgcttttact
     7381 cgttatttga tgtaaacaac gagattgtac gttttacttc gtcgtgaata ccgtcaatgc
     7441 aggcataact cactacttca gcactgttca cccacacaaa gtcactaaaa gcgccttctt
     7501 ctagtttaac atctcccgat tcatacatgg cggcaaactt aaccaaaatt acaggaacac
     7561 catcagggcg gatgaatgca acactcctga gataggttaa ctgtgaagag acggtaatgc
     7621 ccgcctcttc aaaaacctcc cgggcaatta gatcttcaac tgcattatca aaatcaatca
     7681 catcaccgtt caaacgagtt gctttcgtac tatctaagtt tgcccactcg agttttcctc
     7741 ctgggatagc gtatttcccg ggatgaactt tttctctctc ggagcgtttt aaaagcaaac
     7801 agcggccgtc agatttacga tacaccacaa cattggccac aaaataaaac aacttgtcag
     7861 gcttggcgtg atcaaggcta atctttgagg ttggcacgcg atttctcctt cttagaaccc
     7921 ttaggagcca caaatacgat ctttccactg acagtctgat aaatacgcac gacttcgcat
     7981 tccacctcgt gatccaccaa tgtatcgcct ccttcgacaa tgatcatggt gccatctgga
     8041 aggtaaccta acccttgccc aggttctttg cctttctgga taattgccac cattaacgac
     8101 tcacctggca acagtaccgg ccgcagtgcg ctcgctaaag cgttaatatt gagtacccga
     8161 actccacgga tatgcgcaat ttggttcaag ttataatcca gggttaaaag gtgggcatta
     8221 tgtttttgcg aaattcggat aagctcagcg tcaactgttt cttcggcttt cggctcttga
     8281 acaatcgtaa tctttacccg tggctgatta cgaagagaag ctaaaatatt gagcccccgc
     8341 ttcccttttg cccgtttcat tgggtcacgc gcatcagcta gcttttgaag ctcttgtaat
     8401 accacttgtg gcactataag ggttccagga ataaaccctg tttctacgat ttgctcaatg
     8461 cgcccatcaa ttaataccga agtatcgagt acgacgtcaa agctattact tttttcatca
     8521 cgtttaagaa tgacccgcag aaagtcggtc gccgattgtg cttgagccag aaacagatca
     8581 aatatgccaa cggcgataat aacattcaca ataataggaa gccatcggcc aaaattaccc
     8641 ggcaggccat ctaagagtcc actaaccaaa gagccaataa taagccccgt gattaagccg
     8701 actaagccga ggaagaaata aggaaaagag acatgaagta accgtcgaaa aataaagtac
     8761 gcgcacacgg caacagccag ggcgatagaa agaaacaaaa gatcttgttg cgactcaatc
     8821 atcagtattt gcctttcgcc gtctcatctt taagacacca aattcattgc caatatcgcg
     8881 gaccgactta aaggccggag aagccaaata accaagccga gtaacttcgg ttacacggcg
     8941 ttccatttgt cccacacgac ggacctctcc agtcaatcct acttccccat aaaacacctg
     9001 cttggccggt aatgcaaccc ctgatgcggc cgatagcaaa gccatacata tcgctaagtc
     9061 ggctgctcgg tcgctaatac ggtaaccacc cactacgttt aagaagacat ccgctgaaga
     9121 aaaagaggca ccggtccgac tttctaaaat tgccagtaaa atatgaagcc gctgcaaatc
     9181 gactcctgaa ctcgttcgct ttgggtaacc gaaggcggtc ggcacaacca atgcttgaac
     9241 ttcaaccaaa atcgggcgca caccttccaa tacggccgtt agagcagttc caggaacttc
     9301 tcccgtgtgg tcttcaataa aaagtgatga tggatcagca atgctcgaca ttcctttttc
     9361 gcccagctca aagagcccga cctcataggt ggccccaaat cggttcttca tacttcggag
     9421 gatacgcatt tcactccgtg gctcaccttc caggtataaa accacatcaa ccatatgctc
     9481 aagcgtttta ggtccggcca ccgtcccttc tttcgtaata tgtccaacta aaatgatgct
     9541 aatgccgtta gctttcgcaa attcttgcaa acggagtgaa cactctcgaa cctgaaccaa
     9601 actacccgga gtactcggaa aggcatcgtc atagagcgtt tggatggagt caataataac
     9661 taatgttggc ttgagttgcg ttaaggtctg ctggatcgtt ccaagccggg tttcattggc
     9721 aaagaggaat gttggattca gccctagtcg tttagccctt ccagcaactt gagactccga
     9781 ctcttcaccc gaaaaataga ttactgtatg agaatcagcc acatactgtg caacttgcaa
     9841 aagaagagtc gacttaccga tacctggttc accgcctaag agcaccacac tccctgggac
     9901 aataccccca cccaacactc ggtcgaactc cggtaaccgt gtttcgattc tggatttgat
     9961 ccccgtttcg atctgggtgt taagcggttt caaatcgagc gcttgaccag aggtgtttga
    10021 tcgtccactt cctccactgc cccgcacttc tgtcttaaat tctttgaacg tattccactc
    10081 ttggcaaacc ggacatcggc cttgccatgc aattgattct tctccacaat tatcacaaac
    10141 atacatcgta cgtgttttag ccattatgta acgccactcc tttaatcata aactgaggag
    10201 ttttctttcc tctaaactca ttaatcgcca gagtaccgta tatgtcaagc ttagagctta
    10261 aaagcggctt tattgcctcc gctcgaaaat atataactgg aag
//
LOCUS       DASWYB010000084        10979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_1011507, whole genome shotgun sequence.
ACCESSION   DASWYB010000084 DASWYB010000000
VERSION     DASWYB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011507"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="secD"
                     /locus_tag="VGE59_04765"
     CDS             <1..839
                     /gene="secD"
                     /locus_tag="VGE59_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY1074969.1"
                     /translation="PNGSSVVFDQTTRQPIINLVFTDEGADKFYAITSRNVGKPLAIF
                     LDDSPLQVATVQQAISGGQAQISGSFALPEAQETVRLLNAGALPVPLNLKEQRIIGAT
                     LGQESIQRSLVAGAVGLILVAGFMILYYRLLGFVAVLSLLLYTIFSLAIFKLIPVTLT
                     LAGITGFILSIGMAVDANILIFERLKEELREGKSLRRAIEEGFSRAWSSIRDSNASSV
                     MTALILYNFGSSVLLKDSPLHLLLEFSSPSLPRLPSLEPSYTSLSELDYFPVAIKIPE
                     KT"
     gene            883..1890
                     /gene="secF"
                     /locus_tag="VGE59_04770"
     CDS             883..1890
                     /gene="secF"
                     /locus_tag="VGE59_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY1074970.1"
                     /translation="MVTMMEKKKIWLWISALLLIPGIVSLFVWKLQLGIDFKGGSLSE
                     YQTTSLEQGREQVEKIFTEEKVGEIQLQSDVKTSDDIRLYVKSQTLSEDTHRNIVRRL
                     EETNPVVKELSFETIDPQVGRDVTRKALMAIIVASAAIILYLAYSFRGVPKPVSSWQF
                     GVIAVIALLHDVLFIIGFYSLMGHFAGYEVRAEFVTAALTVMGFSVHDTIVVFDRLRE
                     NLKRHPSNTFSQIANMSVAQTMTRSLNTSLTVILVLLAIVLLGGESIREFTLTLLVGI
                     AVGTYSSIFVATPLLDWWQTAGPKVRGFFQRSGNALYLRIKDYQLPSLRRQSARKTQK
                     A"
     gene            complement(2007..2300)
                     /locus_tag="VGE59_04775"
     CDS             complement(2007..2300)
                     /locus_tag="VGE59_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074971.1"
                     /translation="MVKKLFYSLLGVSLILFIFGGFYLWQYFSHVRADYDLLSRDRGS
                     ELIGWGMVLLIIAYGVLIGMFYHHREKAYKWWLILLSPFMFYSVITLGMLLFR"
     gene            complement(2665..6018)
                     /locus_tag="VGE59_04780"
     CDS             complement(2665..6018)
                     /locus_tag="VGE59_04780"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEY1074972.1"
                     /translation="MTMIILIASLVLFIFLGVIAIVSYMRDIEDTNKKIALAEKRHYT
                     LIKIAVPKQNTKSPFAAEQMFSALHGIYRKSEVDQEHFSFEIAATSTSINFYMLLPTH
                     LRDFIEGQVYSQYPTVEIESVSDYSNVSLEDKAIAGAEIKLTKADVFPIRTFANFDVD
                     PLAGITGVLSKINEGEVMSIQAVLSPISDAWQKDGLDYVKKIKEGKSVAPPPPLHKEL
                     QKQGATFLKEIGTGVIKGVAGTAAEAKKEEKKEVKLSGPEEAGLKGIEAKVVKLGYAM
                     TIRVLSIANNEILAQTRVESVLGAFKQFNLTNLNGFDLGEITTDASFLRTFRTRSLGS
                     KPFRMNVEEIASVFHLPDASVETPNIAWAGSKKGEPPQNLPIDGEVPAAELTTFGMTS
                     FRNRKIKFGIKQIDRLLHFYIIGKTGTGKSTLIENMIYDDIKEGRGVAVVDPHGELID
                     HILDFIPEERVKDVVYFNPADTDHPLAFNLLEDIENADMRGIIASGLMSIFTKLWAGV
                     WSARMEYILRNSILAVLEVPNATLLSIMRVLNDPVYRKYVMSHVTDPVVRDFFINEYD
                     KYDAKFRQEAIAPIQNKVGQFLSSSTIRNIVGQPKSTFNLLEVMDSGKIFLVNLSIGR
                     IGEDSSKLLGSMLITKLQLAAMSRAKKLATERREFFLYVDEFQNFATDSFATILSEAR
                     KYKLGLILANQYIAQIPELVADAIFGNVGSMVSFRVGPTDADKLVKEFVPVFDANDLI
                     NLPNRNIYTKMAIDGVTSRPFSAHTLPPRGLATDNKEKIIELSRQTYSRRRDDVEREI
                     NDLAQADYRPESEKEYDDFRQYPYILGSTLYKEFSAKGGQRWYFGQPPEFAIEELDAA
                     GVPIPEEMRAAAQLVGKEPKKESDTPPTAPASVEAETPKTITPVEKVSAHDVVPYKEP
                     EILPAVMNDPEPQMSVVQPDSPVSVVPQESPHPTSENSEIKKKRKRKRKKKNNGNGGE
                     GQALVSSHEHVPSMPSSDPAQEISYEDPDHGMATSLPESEYAAVSEPEVYESYEEQSP
                     IEYPAEVIAEEVYHYPQESYAPIPQDESMAASTPYQQVEPSGAGEWPTEEGGVTLLTE
                     GTEYRDVPESEPSAVQRPQESGWMPLDEL"
     gene            complement(6077..8545)
                     /locus_tag="VGE59_04785"
     CDS             complement(6077..8545)
                     /locus_tag="VGE59_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEY1074973.1"
                     /translation="MMFLPLIITIGLGVLGYWGWQVMSRRWRLTRAVRRAKDFVLLEV
                     LVPKEINKAENETPPQLKELISVMEQFLTNLSGYYTPGLKGLGGNQPVFSLEIVARHS
                     EIRFFIGTPAEYESAIEKQILGYYPEAQVERSKSFRIMEKGYAMAAGQLKLMRKFIFP
                     IQTYMDVESDPLQSITTSLSKMGPDSRSLIQILIQPTDNAWRNQSDMAIRKIQEGKDV
                     SMVSGPWWVKLLHNSGKAVTENTAPKDPTKPYIALSPAQQEMQKLLQSKGSKLGFSTH
                     INIVAVAPETGEAQFQLANILAAFSQYNTAQGNGFKAKRIKQMRDFLIHVMLRTPEKR
                     GMILNTTEIASLFHMPNYSIDTPGIVWLLAKFAAAPPNTPTEGILLGENVFRGQRKNI
                     YLKPEDRMRHVYAVGQTGTGKTTLFENMILQDIREGKGVCYIDPHGAAIENILSRIPR
                     ERAKDVILFDPGNMDRPMGLNLLEWHRPEEKDFLINEFILMFYKLFDPNRTGMVGPQF
                     EHWARNAALTIMSDPEGGTLIEIPRLFTDDKFRERILSYVQDPVVLRFWNEQMAKTAD
                     FHKSEMFNYFISKFGRFMTNELMRNIIGQPKSAFDVREAMDSGKILLVNLSKGKIGDI
                     NAQMLGMILVAKINSATLARQDMLAQGQQPRDFYLYVDEFQNLATDTFGTILSEARKY
                     RLSLNITHQYIAQLPEDVRNAVFGNVGTVVAFRVGVEDAKFLVSQFTPAFSEGDLMNI
                     DRYNAYVRYLVDNAPIRPFSIRTMKDDSPTDPQLRNAIVELSNLSYGRNRQEVDQQIR
                     NRAQLAEGVGVAEAVTTSTGKKSS"
     gene            complement(8546..9154)
                     /locus_tag="VGE59_04790"
     CDS             complement(8546..9154)
                     /locus_tag="VGE59_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074974.1"
                     /translation="MDFTTLDGAQSAPLPTAEVPVSVTPEVSLQATSVEDGAINVPEA
                     NLQAPLTVEGTGASIPSTEQAPNNAVLLGQPAGQNVGVPTPPSMGAVDGVLPPTTSRP
                     ELQSGGVGSEKVFTTETAPAAPSEPLPLSSPPVITPPQPAPIMAVAPVPPIAQMQPQP
                     TPPRRLAPDTLIRDILSPRPKEQLDDLLFRLELAVMSQSEQV"
     gene            complement(9215..10375)
                     /gene="tgt"
                     /locus_tag="VGE59_04795"
     CDS             complement(9215..10375)
                     /gene="tgt"
                     /locus_tag="VGE59_04795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY1074975.1"
                     /translation="MKFNLLKTDTKTRARRGELHLAHGVVQTPIFMPVGTVGSVKAVS
                     NEDLDALGAQIILGNTYHLHLRPTSEFIKDHFDSLHNFMGWKKPILTDSGGYQVFSLG
                     LRAEARGEGPQLVKITEEGATFYSHIDGSKHLFTPERVIDIQRNLGSDIMMPLDACPD
                     GNADHMAIKKAMEQTHRWAQRGIEYWNKVKGESLQNYFGIVQGGPFADLRKESAQFIS
                     SLPFDGIAIGGVANGGESKDKMTEAVEYAVPYIPHDKPRYLMGVGEPIDMIKAVSQGI
                     DMFDCVLPTRLGRHGVAWVGDEKRGFDSLDIAKSIYKTDKNVLDEICDCPACRNGYSR
                     AYIHHLLKEKEILGIRLLTLHNLRHVLSLFERMRQSLEEGRFSEQFKSYIAE"
     gene            complement(10390..10692)
                     /locus_tag="VGE59_04800"
     CDS             complement(10390..10692)
                     /locus_tag="VGE59_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074976.1"
                     /translation="MEGIVSYPQWFAFWAFVGTGGALCFAGWILVGLTQLTLSCSRLG
                     SAGSPPTKSQWILRVALLLFCVGLAINSWSNLRHFQSQNKTVRSRGPSFLFLQGIF"
     gene            complement(10695..10919)
                     /locus_tag="VGE59_04805"
     CDS             complement(10695..10919)
                     /locus_tag="VGE59_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074977.1"
                     /translation="MEYLVWLRSSLVGLGVGLLLTMGVLFAVSLLVTLNAKKEYLGRA
                     QKFLQITLAGVFISLAFLVIVVSAQAGAGR"
BASE COUNT         3002 a         2647 c         2332 g         2998 t
ORIGIN      
        1 aaccaaatgg cagctccgta gtttttgatc aaaccacacg gcaaccaatc atcaatcttg
       61 tctttactga tgaaggtgct gataaatttt atgctatcac cagccgaaat gtcgggaaac
      121 cacttgccat ttttctcgat gactcaccac tccaagttgc caccgtgcaa caagccattt
      181 ctgggggaca ggcccaaatt agcgggtcat ttgcgctccc tgaagcccaa gagacggtgc
      241 ggctcttaaa cgctggtgca cttcctgttc ccctaaacct taaagaacaa cgcatcattg
      301 gtgccacact cggccaggaa tctattcagc gaagcctcgt cgccggagcg gtaggattaa
      361 ttcttgtcgc tggcttcatg attctttatt atcgactact cgggttcgta gcggtcttgt
      421 cactcttgct atatactatt tttagccttg cgatctttaa actcataccg gtaaccttaa
      481 cccttgccgg aatcacaggc ttcatactct ccatcggtat ggccgttgac gcgaatatcc
      541 ttatctttga acgcttgaaa gaagaattgc gcgaaggaaa gtcactccgt cgggcaatag
      601 aagaaggatt ttcccgtgcc tggtcgagta tccgagactc taacgcatct tccgtgatga
      661 cagcgttaat cctttataat tttggatctt cagtcttatt aaaggattct cccttacact
      721 tattattgga attctcgtct ccctctttac cgcgattacc gtctctcgaa ccctcttata
      781 cctcattgtc ggaactcgat tacttcccag tggcgataaa gatacccgag aaaacgtaaa
      841 aggggagcgt agtcttagaa aacgagcaac aaggatgcaa cgatggtcac aatgatggaa
      901 aaaaagaaaa tttggttgtg gatttccgct ctactcctta tacctggtat tgtgagtttg
      961 ttcgtgtgga aactccaact tggcatcgac tttaaaggcg ggagtttatc cgaatatcaa
     1021 acaacatctt tagagcaggg gagagagcag gtagaaaaga tcttcactga agaaaaagta
     1081 ggcgagatcc aacttcagtc ggatgtaaaa acttcggatg acatccgtct gtatgtaaag
     1141 tcgcaaacat tatctgaaga cactcacaga aatattgtgc gacgccttga agaaacaaac
     1201 ccagtggtga aagaattgag ctttgaaaca attgacccac aggtaggaag agatgtgact
     1261 cgcaaagccc ttatggcaat tattgttgca tcggccgcta tcattttgta cctcgcttac
     1321 tcgtttagag gagtaccaaa acccgtctca tcctggcagt ttggcgtgat tgcggtgatt
     1381 gccttactcc atgatgtatt gtttattatt ggattttact ctcttatggg acactttgca
     1441 ggctatgagg tgcgagcaga attcgttacg gctgccttaa ccgtgatggg cttctcggtt
     1501 catgatacca tcgttgtttt cgaccgactg agagagaacc tcaaacgcca tccatcgaat
     1561 actttttctc aaattgctaa tatgagtgtc gcacaaacaa tgacccgctc tcttaatacc
     1621 tctctcacgg ttattctggt gctcttagcc atcgtattac tcggcggtga gagtattcgc
     1681 gagtttacgc taaccctcct tgtgggtatt gccgttggga cctattcatc tattttcgtg
     1741 gcaacaccgt tactcgattg gtggcaaacc gccggaccga aagttcgagg attcttccag
     1801 cgaagtggaa atgccttgta tctgcgtatc aaagactacc aacttccaag ccttcgtcga
     1861 caatcagctc gcaaaaccca aaaagcgtaa ccttcgtcgt cgaccaccat tgatatttca
     1921 catcgggcga tagattagtc gtccaatata aaatcggcgc ttaaaatcga ttctagagcc
     1981 tttaaacctg ttggatggct ctagcatcag cgaaaaagca acatgcctag agtaatcaca
     2041 gaataaaaca taaacggcga taataaaatc agccaccact tatatgcctt ctcacgatgg
     2101 tgataaaaca taccgataag tacgccatac gcaataataa gtagtaccat accccaacca
     2161 attaattcac ttccacgatc ccgagagagg agatcataat ccgcccgaac gtgagagaaa
     2221 tattgccaga gataaaaccc gccaaaaata aaaagaatga gactcacacc cagaagactg
     2281 taaaataatt tcttaaccat acataaacac tatcaataac tcatcgaaag aaaaagagcg
     2341 ggcgtgcagg cgaagagagt gggggagagg aaaaagaagc ccggatttat ccgctcgaca
     2401 cgtgcgaaag tgttcaccag acaaaaacat agcgtcgcac aatgtttatt ttgcgacgta
     2461 ataagccaga tttcatccag tacggctaat tgttgtttac tggcttagag gctaaattta
     2521 ggattatttt ttgccttttt acttccctct tttgtgacag cacaaaaaac tttcagacta
     2581 aagaaaaatc atttaaaaca ctatgtgcga cgcattttat agcgtcgcac aatatttcat
     2641 ataagagggt agaaaagaga gggcctacaa ttcatccaga ggcatccaac cactctcttg
     2701 cggccgctga accgccgatg gctcactttc tggtacatct cgatactctg ttccttcggt
     2761 aaggagagtc actccccctt cttctgtcgg ccactcccct gccccactgg gctcaacctg
     2821 ttgataaggc gtagaagcag ccatagactc atcttgaggt ataggagcgt atgattcttg
     2881 aggatagtga tacacctctt cagcaataac ttccgcagga tactcaattg ggctctgttc
     2941 ttcgtagctc tcataaacct caggctcaga aactgcggca tattcacttt ctggtaatga
     3001 ggttgccata ccatgatcag gatcttcata ggatatttcc tgggctggat cagacgaagg
     3061 catggaaggg acatgctcgt gagaacttac gagtgcctgc ccctcaccac cgtttccgtt
     3121 atttttcttt ttgcgcttcc gttttcgctt cttctttatt tcgctatttt ctgatgtggg
     3181 atgaggcgat tcctgcggga cgaccgaaac tggagagtca ggctgtacaa cactcatctg
     3241 tggctctgga tcattcataa cagcggggag tatctcaggc tctttatatg gaacgacatc
     3301 gtgcgcagag actttctcaa ccggtgttat agtttttgga gtttcagcct caacactagc
     3361 tggagcagtc ggcggtgtgt ctgattcctt cttaggttct tttccaacta actgcgccgc
     3421 ggcccgcatt tcctcaggaa ttgggactcc cgctgcatcc agttcctcaa tcgcaaattc
     3481 tggtggctga ccaaaatacc agcgttgacc acccttggcc gagaattctt tatagagtgt
     3541 gcttcccagg atataggggt actgacgaaa atcgtcatac tctttttcac tctccggccg
     3601 gtagtcggcc tgggcgaggt cgttaatttc tcgctcaaca tcatcacgcc gacgcgagta
     3661 cgtctgacgg gacagttcga taatcttttc tttattatcg gtagctaatc cgcgaggagg
     3721 caaagtatga gccgagaacg gacgtgaggt caccccatca atagccattt tggtataaat
     3781 atttcggttc ggcaggttga taagatcatt ggcatcaaaa acaggcacaa actctttgac
     3841 aagtttatcg gcgtcagttg gcccaacccg gaacgaaacc atactcccaa cgttaccaaa
     3901 gatcgcatcg gccaccagtt cgggaatttg ggcaatgtac tgattcgcga gaattaaccc
     3961 aagcttatac ttgcgcgcct ctgacagaat agtggcaaaa gagtcagtcg cgaagttttg
     4021 gaactcatct acgtagagaa agaattctcg tcgttcggta gccagcttct tcgctcggct
     4081 cattgccgcc aattgcaatt tcgtaatcag cattgaaccc aaaagctttg agctgtcttc
     4141 tccaatccgt ccaatcgata ggttaaccaa gaagatttta cccgaatcca taacctcgag
     4201 taagttaaaa gtcgatttcg gctggccgac aatattacgg atcgtagagg atgaaaggaa
     4261 ttgcccgacc ttgttctgaa ttggcgcaat tgcttcttgg cgaaactttg catcgtattt
     4321 gtcgtattcg ttaataaaga agtcacgaac cacagggtct gtcacgtgag acatcacgta
     4381 tttacgatat acggggtcat ttaagacacg catgatactc aagagtgtcg cattcggaac
     4441 ttctaggacg gccagaatcg agttccgaag gatatattcc attcgagcac tccagactcc
     4501 ggcccacagt ttggtaaaaa tactcattaa acccgaggca ataatgccac gcatatcggc
     4561 attttcaata tcttcaagaa ggttaaaagc taaaggatgg tcagtatcgg ctgggttaaa
     4621 gtacacaaca tctttaaccc gttcctctgg gataaagtct aagatgtgat caataagctc
     4681 accgtgcgga tcaacaaccg ctacgcctct tccctctttg atgtcgtcgt aaatcatatt
     4741 ctcaatcaaa gttgacttac ccgtaccagt ctttccaata atgtaaaagt ggagcaaacg
     4801 gtcgatttgt ttaatcccaa acttaatctt ccggttacgg aaactggtca taccaaaagt
     4861 ggtaagttct gcagcaggaa cttccccgtc aattggtaag ttctgaggcg gctccccttt
     4921 tttacttccg gcccatgcaa tattaggagt ttcaaccgaa gcatccggca agtgaaatac
     4981 cgatgcaatt tcttcgacat tcatacgaaa cggtttgctg ccaagactgc gggtgcggaa
     5041 tgtccgtagg aatgacgcat cggtcgtaat ttcaccaaga tcaaatccat tgaggtttgt
     5101 gaggttaaat tgcttgaacg cccctaaaac cgattcaact cgtgtttgag cgagtatctc
     5161 attattcgcg atagagagga cacgaatagt catcgcatac cccagtttga caactttggc
     5221 ttcaatccct ttcaaaccag cctcttctgg gcccgaaagt ttaacttctt ttttctcttc
     5281 tttttttgcc tcagcagccg taccagcgac gcctttaatg acgcctgtac caatttcttt
     5341 caggaaggta gctccctgtt tttgaagctc tttatgaagc ggaggtggcg gcgcaacact
     5401 cttcccctcc ttaattttct tgacgtagtc taatccatct ttttgccagg catcactaat
     5461 aggactgagt actgcctgaa tactcataac ttcaccttcg ttaattttgc tcaacacgcc
     5521 ggtaataccg gcgagcggat caacgtcgaa gttagcgaaa gtccgaattg gaaagacatc
     5581 tgccttagtg agtttaattt cggctccggc aatagcctta tcctctaaag aaacgttcga
     5641 gtaatccgag accgattcga tttctaccgt tggatattga gaataaacct ggccttcaat
     5701 aaaatcgcgt agatgcgttg gaagaagcat gtaaaagtta atcgaggtgc tggtcgcggc
     5761 aatttcaaag ctaaagtgct cttggtcgac ttcgctcttt ctatagatac cgtgcagcgc
     5821 tgagaacatt tgttcggccg caaagggtga ttttgtgttt tgctttggta cggcgatctt
     5881 aatgagcgta tagtgtcgtt tctcggctaa tgctattttt ttattcgtgt cttcaatatc
     5941 gcgcatgtaa gagacaatgg cgataacacc tagaaaaata aacagaacta atgaagcaat
     6001 cagaattatc attgtcatag aatggcctct attctactct ttaaagacat atttttcaag
     6061 gatctgtgat ggaatgtcat gatgattttt ttccggttga tgttgtaaca gcttctgcca
     6121 caccgacccc ttcggctagt tgtgcccggt tacgaatttg ttgatccact tcttgtctat
     6181 ttcggccgta acttaaatta gaaagttcaa cgattgcgtt acgaagctga gggtcagtcg
     6241 gtgaatcatc tttcattgtg cggatactaa acggacgaat cggcgcatta tcgacaagat
     6301 aacgaacata cgcgttatag cgatcaatgt tcatcaaatc cccctcagag aaagctggcg
     6361 taaactgact gactaaaaac tttgcatctt ccaccccaac gcgaaatgca acaacagttc
     6421 ccacattgcc aaaaaccgca ttacgaacat cttctggtaa ctgggcaata tattgatgcg
     6481 ttatattcaa gctcaaacga tattttcggg cttcggaaag gattgttcca aacgtgtcgg
     6541 tagctaggtt ttgaaattca tcaacataga ggtaaaagtc acgcggctgt tgcccttgtg
     6601 ctagcatatc ttgtcgagca agcgtggctg aattaatttt tgcgacaaga atcataccaa
     6661 gcatctgagc gttaatgtcg ccaattttcc ccttcgataa gttaaccagc agaattttac
     6721 ctgagtccat tgcttcacga acgtcaaagg cggattttgg ctgaccaata atattacgca
     6781 tgagctcatt ggtcataaaa cgaccaaact tagaaataaa gtagttaaac atttccgact
     6841 tatgaaaatc agccgtcttc gccatttgtt cattccagaa tcgtaatacc accggatcct
     6901 gtacataaga taaaattcgc tcgcgaaatt tatcgtccgt aaataagcga ggaatctcga
     6961 ttaaagttcc tccctccgga tcggacataa tcgttaaggc agcattacgc gcccagtgtt
     7021 caaactgcgg cccgaccatt cctgttctat taggatcaaa aagtttatag aacattaaaa
     7081 taaactcgtt aatcaaaaag tccttctctt caggccgatg ccattcgagt aagttaagcc
     7141 ccatagggcg atccatatta ccagggtcaa aaagaattac atcttttgcc cgttctcgtg
     7201 ggatacgcga caaaatattc tcaatcgcag ccccatgggg atcgatataa cataccccct
     7261 tcccctcgcg aatatcttgt agaatcatat tctcgaataa tgttgtttta cctgttcccg
     7321 tctgaccaac agcatagaca tgacgcatac gatcttctgg cttgagataa atatttttac
     7381 gctgcccacg gaatacgttc tcccctagaa gaattccttc ggttggcgta tttggcggag
     7441 cggcggcaaa tttagccaat aaccatacaa ttccaggtgt atcaattgaa tagttcggca
     7501 tatggaaaag cgaagctatt tctgttgtat taaggatcat tcctcgtttt tctggagtcc
     7561 gtaacatgac atgaatcaga aaatctcgca tttgcttaat acgcttcgcc ttaaacccgt
     7621 ttccttgcgc ggtattatat tgcgaaaaag cggccagaat attagcaagc tgaaattgag
     7681 cttctccggt ttcaggtgcg acggctacaa tattaatatg agtcgaaaaa ccgagtttcg
     7741 atccttttga ctgaagaagt ttttgcatct cctgttgtgc aggagataag gcaatatatg
     7801 gtttcgtggg atctttcggc gccgtattct cagtcacggc tttacctgag ttatgcaata
     7861 atttaaccca ccaaggtccc gacaccatcg agacatcttt cccctcctga atttttcgaa
     7921 ttgccatatc ggactgatta cgccaggcat tgtcagtggg ctgaattaag atttgtatca
     7981 gagaccgaga atcaggcccc atctttgata gagaagttgt gatcgattgg agcgggtcgc
     8041 tctctacatc catataggtt tggataggaa aaataaattt acgcataagt tttaattggc
     8101 cagcagccat tgcgtagcct ttttccataa tgcggaatga tttacttcgt tcaacctggg
     8161 cttccggata atagccaaga atttgttttt caatcgccga ttcatattca gcgggtgtcc
     8221 caataaaaaa ccggatctca ctatggcggg caacaatttc cagactaaaa accggttgat
     8281 ttccacccaa accttttaac cccggcgtgt agtaaccaga aagatttgtt aaaaattgct
     8341 ccatgacgga aatgagctct tttagctgcg gcggagtttc gttttcagcc ttatttatct
     8401 ctttaggaac caaaacctca agcaaaacaa aatccttagc ccgtcgtacg gctcgggtca
     8461 aacgccatcg acgtgacatg acttgccatc cccaatagcc gagaacgcca agtccaatgg
     8521 taataataag agggagaaac atcatttaaa cctgctcgct ttgtgacata acggctaact
     8581 caagtcgaaa aagtagatca tcaagttgtt cttttggtcg aggcgataaa atatctcgga
     8641 tgagtgtatc aggggccaga cgacgaggag gggttggctg cggctgcatc tgagcaatcg
     8701 gaggaacggg agcaacagcc attataggcg ccggctgcgg cggcgttatt actggcggac
     8761 tagaaagagg aagcggttcg gaaggagccg caggagcagt ttcggtagta aaaacttttt
     8821 ccgagccgac tcctccgctt tgtaactccg gacgacttgt cgtgggaggc aatacaccgt
     8881 ctacagctcc cattgaaggc ggtgttggca cacctacatt ttgtcctgcg ggctgcccta
     8941 gcaacacagc gttatttgga gcttgctctg tcgaaggtat ggatgcgccc gttccttcaa
     9001 ccgtcaatgg agcttgaaga tttgcctcgg gtacgtttat tgccccatcc tcaacagagg
     9061 tcgcctgtag cgaaacctct ggtgtaacgc ttactggcac ctctgcagta ggcagaggtg
     9121 cgctttgtgc cccatccaac gtcgtaaaat ccataatttc atggtagcac actcgaataa
     9181 aaaaagaacg cccagacatc tgggcgttct ttttttattc ggcgatgtaa cttttaaatt
     9241 gttccgaaaa tcggccttct tcaagactct gccgcatgcg ctcaaacaga cttaaaacat
     9301 gcctcaggtt atgaagcgtc aataaacgga tacctaaaat ttctttttct tttaacaaat
     9361 gatgaatata ggcacgagaa taaccattgc gacaagccgg acaatcgcag atttcatcca
     9421 aaacgttttt atcagtttta tagatagatt ttgcaatatc gagcgaatca aatcctcgct
     9481 tctcgtcgcc cacccatgca acgccgtgtc gccctagacg agttggtaag acacagtcaa
     9541 acatgtcgat accttgcgat acggctttaa tcatatcaat cggctcgccc acgcccatca
     9601 ggtaacgagg tttatcatga ggaatatagg gaactgcgta ctctaccgct tcggtcattt
     9661 tgtccttgct ctctccccca tttgccaccc caccaatagc aataccatca aatggcaggc
     9721 ttgaaataaa ctgcgcggat tcttttcgca agtccgcaaa cggtccacct tgtacaattc
     9781 caaaataatt ttgcaggctc tcccctttta ctttattcca gtattcaatc ccccgctgtg
     9841 cccagcgatg cgtttgctcc atagctttct taatagccat atgatccgca tttccgtccg
     9901 gacaggcatc aagcggcatc ataatatctg accctaagtt tctctgaata tcaattactc
     9961 gttcaggtgt aaaaagatgc ttgctcccat caatgtgaga ataaaaagta gcgccttctt
    10021 cggtgatttt gacaagctga ggaccttctc ctcgagcttc tgcccgtaat cccaggctaa
    10081 atacttggta accaccactg tcagtcaaaa taggcttctt ccaacccata aaattatgca
    10141 aagaatcaaa atggtcttta ataaattcgc tcgttggccg gaggtgtaag tgataggtat
    10201 tgcctaaaat aatttgtgcc ccaagcgcat ctaagtcttc gttcgagacc gcctttacgc
    10261 tccccacggt tcccaccggc ataaaaatag gcgtttgaac gacgccatgg gctaaatgta
    10321 attctccacg tcgagcgcga gtttttgtat ctgttttaag taaattaaat ttcatacttg
    10381 cctattatac tagaaaatac cctgtaaaaa aagaaacgac ggtccgcgac tgcggaccgt
    10441 cttgttctgt gactgaaaat gccgcaggtt gctccaggag ttgatcgcca gaccaacaca
    10501 gaacagcagc agagccaccc ggagtatcca ctggcttttg gttggcggag aacccgcact
    10561 acccaaccgg ctgcaggaaa gtgtaagctg ggtcagcccc accagtatcc aaccggcgaa
    10621 gcaaagtgcg ccaccggtac caacgaaggc ccagaacgca aaccactgtg ggtaactaac
    10681 aatcccttcc atctctatct ccctgcgccc gcctgggcgc tcacgacgat taccagaaaa
    10741 gcgaggctta taaagactcc tgcaagtgta atttgcaaga acttttgcgc tcgtccaagg
    10801 tactcttttt tcgcgttaag agtcactaac agggagacag caaaaagtac tcccatagtc
    10861 agcaacaagc caaccccgag ccctacaagg ctcgatctga gccagacaag gtactccatc
    10921 tcccccactc cttaactaac acagatgcgc atattatatt ctattttctt cattttggc
//
LOCUS       DASWYB010000085         6147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_44946, whole genome shotgun sequence.
ACCESSION   DASWYB010000085 DASWYB010000000
VERSION     DASWYB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44946"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3005
                     /locus_tag="VGE59_04810"
     CDS             <1..3005
                     /locus_tag="VGE59_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY1074978.1"
                     /translation="LQAVETIDGPVMVLAGPGTGKTQVLAFRIAEILQQTDVHPRNIL
                     ALTFTESGVVAMRERLVSLIGVTAYGVGIYTFHSFANRVINDAGAEFYKSHSLDPIDD
                     ITQLKLIIRLIDEVNNERLRPIRAPYFYVKPIIHSIKSLKNEGVSPDRLRTLCREEIT
                     TLETSDESISKSGKNKGGLKQSVKDKIERLERSAALADIYERYEAEITERGLYDYEDM
                     ILFVIKSFQENPSLKAQYQEQFQYILVDEYQDTNNAQNTLVRSLCDYFESPNLFVVGD
                     DKQSIYRFQGASMANLLNFLEWYPDATLISLRQNYRSGQPILDNAHTLISSNTEQLTK
                     VIPSIHSELTAQFSEAKVIFTPYTTADKEALGVVQGIKALLAQGVKASEISVIYRENS
                     EAASFTDLLARQGIPFHLEAGNNVLKDPDVRQLLNILELTHNPDNEMALFRLLHAPYS
                     GIETRDLITLGRWRKQQRTTWTKLLAGDCPETDVPINDWSCFQTLKHKVEAWHRYSLQ
                     HNLGDTVEHVFLDSGFLAHVMQQHDHMDRLHRVRCFFDEIKSIMTSVNFATLDDLFER
                     LEIRQTYNLALIPSPLSERSDDAIRLMTSHKSKGLEFEYVFVPHFYDGLWSNGRKSEL
                     ISLPEGIVPHHQESDEQAIQEERRLFYVALTRAKKAVFLSYSELDAANKKLLPCQFLT
                     ELNSVTTAAPVATNLMPITAFFSPIQTHFVEETSKAYLRDIVTKQSITPTGLNTFLTC
                     PLEYLYKDVYCIPGVREAFQAYGTAIHKALEMWGNWSKTGAHDFGLASILSVFTETLV
                     KEGLTQDEVNRYQRLGEDVLTAYYQEFALGWVPPLATEYSFTPHGVLLDGRIPITGKI
                     DKIEPIKGSKHVRVIDYKTGRVKSRNEIEGNTASSDGDYKRQLVFYAVLAESDPFFPY
                     TIGECALSFIDDQKKFTTEVFEITKQEKDELRELIRNIYAEITSLNFIHTPHKQSFNK
                     GESLCDLLRSDMLTGLPATPASMQESAL"
     gene            3068..4420
                     /locus_tag="VGE59_04815"
     CDS             3068..4420
                     /locus_tag="VGE59_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY1074979.1"
                     /translation="MDDLVKKVVESPARAVDDFVAVANSPRYKKPIRIAKKYWGELGP
                     GLTTGASDDDPSGIATYSQAGAIFGFSFLWMSLYLIPFMVVAQEMCGRIAHVTGRGIS
                     GVLKQHYSKRIVRSTVLMLFAANTLNIGANIAAMAASTRLVFPGLPFTVLAVFFTLLS
                     LILQISMPYSRYEKFLKYLTLVLFAYIITAFLIRADWAEVIRHTVQPHFIFTKDGIFM
                     MTAIVGTTISPYLFYWQASQVIEQQREEYGRKTLTSLAGTNAKEISLMRKDVTTGMVF
                     SNIVMFFIIAVAGTVLMPKGITIDTAADAAQALRPLAGDFAFLLFAIGIVGTGMLAIP
                     VLAGSASYAFSEAFSLNHGLDKQLKRAYGFYGVIILSMGIGLLINFLGIHPIKALIYS
                     AVINGLVAPVLLTLILLVANNHKIMGEWKNGPVSNILGWFITIIMYIAGIATLWGMFF
                     "
     gene            4501..4914
                     /locus_tag="VGE59_04820"
     CDS             4501..4914
                     /locus_tag="VGE59_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074980.1"
                     /translation="MPSWVFGVGAVALLLGLVCICMSQRALRIFGQGVLVIAAYWMAV
                     IFITELPLILLDLIVTGVKMAGTAGIDWLKGFVGIKPQGVNAAELVFVFLKPSLVLFL
                     LARAGLRFAQIIESAIVEDQTVLQTLRESRHIRWY"
     gene            complement(4999..5289)
                     /locus_tag="VGE59_04825"
     CDS             complement(4999..5289)
                     /locus_tag="VGE59_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074981.1"
                     /translation="MSLSEVPNVTAQQIMGGNIGIMNAIMHLTRLPKFKAAGEATTLK
                     LLVLATSDDPATPLTGPDLWDRLKELFPLEGGGWDINQLVQAMEQCLDDETT"
     gene            complement(5434..5682)
                     /locus_tag="VGE59_04830"
     CDS             complement(5434..5682)
                     /locus_tag="VGE59_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074982.1"
                     /translation="MSKKGERTKPTVMDHISQIAILGGAIGMMFITSGHWIGFVLSLV
                     TQPFWFYTSWRNRQWGVFTGSVIYTIAFLVGLSRALHK"
BASE COUNT         1528 a         1416 c         1488 g         1715 t
ORIGIN      
        1 agcttcaagc cgttgagacg atcgacggtc cggtgatggt gcttgccgga ccgggaaccg
       61 gtaagacgca agtgctcgct tttcgaattg ccgaaatttt gcagcaaacc gatgtccatc
      121 cccgcaacat attagccctt accttcacgg aaagcggggt tgtggcgatg cgtgagcgct
      181 tagtttcgtt aattggcgtt actgcctacg gagtcggtat ttataccttc cacagttttg
      241 ctaaccgggt catcaacgat gctggtgcag aattttataa gagccattct cttgacccga
      301 tcgacgatat cacgcagctt aaactaatca tccggctgat tgatgaagtc aataacgaac
      361 gtttgcgtcc tattcgtgcc ccctattttt atgtaaagcc gattattcat tcaattaaga
      421 gcctaaaaaa tgaaggcgtg tcgcctgatc gtttgcggac gctctgccgc gaagaaatca
      481 cgacgttaga aacgagcgac gaatccatta gtaagtcggg taaaaataaa ggtggcttaa
      541 agcaatctgt aaaagataaa atagaacgcc tcgagcgcag tgctgccctg gctgatattt
      601 acgaacgcta tgaagcagaa attaccgagc gtggcttata cgattacgaa gacatgattc
      661 tttttgtgat taaaagcttc caagaaaatc caagtttaaa agcgcagtac caagagcagt
      721 ttcagtacat cttagtggat gaataccaag atacgaataa cgcccaaaat accttggtgc
      781 gttccctatg tgattacttt gaatctccca acctgtttgt ggtaggggat gataagcagt
      841 cgatttatcg ttttcagggc gcatcgatgg caaacctgct caactttttg gagtggtatc
      901 ctgacgcaac gttaattagt ttgcgtcaaa actatcgtag tggccaacct attttagata
      961 atgcgcatac tttaatttcg agcaataccg agcagttaac caaagttata ccttccattc
     1021 acagtgagct tacggcccaa tttagtgagg caaaagttat ttttactcct tatactacgg
     1081 ccgacaaaga agctttgggg gtggtgcagg gaataaaggc attactagcg caaggggtca
     1141 aagcttctga gatttccgtt atttatcgtg agaatagcga agcggcttct tttacggatt
     1201 tacttgcacg ccaaggcatt ccgtttcact tagaggcagg caacaatgtt ctcaaggatc
     1261 ctgatgtaag acagctatta aacatcctag aattaacgca taaccctgat aatgagatgg
     1321 cgctctttcg gttattgcac gcgccatata gtgggattga aactcgggat ctgatcaccc
     1381 tgggacgatg gcgaaagcaa caacggacga cgtggaccaa attacttgcc ggggactgcc
     1441 cggaaaccga tgtccctatc aacgactggt cgtgtttcca gacgttaaag cataaagtgg
     1501 aagcgtggca tcgctattcg ttgcagcata acttgggaga tacggttgag catgtctttt
     1561 tagattcagg atttttggct catgtcatgc aacaacacga tcatatggat cgcttacatc
     1621 gggtccgctg tttcttcgat gaaataaaaa gtattatgac atcggttaac tttgccacgc
     1681 ttgatgacct gtttgaacgt ttggaaattc gccagacgta taacttagct ctgattcctt
     1741 ctcctctttc agagcgcagt gacgacgcaa ttcgactgat gacttctcat aagtcgaaag
     1801 gcttagagtt tgaatatgtc tttgtgccgc atttttatga tgggctatgg agtaacggcc
     1861 gtaaaagcga actcatttct ctcccggaag ggattgtccc acatcaccaa gagtcggatg
     1921 agcaagcgat ccaagaagaa cggcgcctct tttatgttgc actaactcgt gccaaaaaag
     1981 ctgttttctt atcgtattca gaactcgatg cggccaacaa aaaacttctt ccctgccaat
     2041 ttctgaccga gttgaactcc gtgactactg ctgctcctgt tgccaccaac cttatgccta
     2101 taacggcgtt cttttcacca atccaaaccc actttgtaga agagacgagt aaggcctatt
     2161 tacgagatat tgttaccaag caatctatta cccccacagg gctcaataca tttttaacgt
     2221 gtcctttgga atacctctat aaagatgtct actgcattcc tggagtccgg gaggcgtttc
     2281 aggcttatgg aacggccatc cataaagctc ttgagatgtg gggaaactgg agcaagacgg
     2341 gggctcatga ttttggtttg gccagcattt tatcggtctt taccgaaaca cttgtgaagg
     2401 aaggtttaac gcaggacgaa gtaaatcgat atcagcgact cggggaagat gtgctcacgg
     2461 cttactatca ggagtttgcg ctgggttggg ttcctccttt agcgaccgaa tacagtttta
     2521 ctcctcacgg cgtacttctg gatggcagga ttcctatcac cggcaaaatt gacaagatag
     2581 aaccaatcaa aggttcaaag cacgtccggg tgatcgatta taaaactggt cgcgtaaagt
     2641 cacgtaatga aatcgagggt aatacggcct ccagtgacgg agattacaag cgtcagttag
     2701 tcttctatgc ggtattagca gaatccgatc cgtttttccc ctatacgatc ggggagtgtg
     2761 ctctctcgtt tattgatgat cagaaaaagt ttacaaccga agtcttcgaa ataacaaagc
     2821 aagaaaaaga tgagctacgg gagctgatcc gaaatattta tgccgaaata acttcattaa
     2881 actttattca tacgccgcac aagcagtcct ttaataaagg cgagagcctg tgtgatctgc
     2941 tacgatcaga catgcttacc gggttaccag caacacctgc gagcatgcaa gaatcggctc
     3001 tctagacacc tagtgtccac catactacac tgggaataca gaattgtttt ctcccaggag
     3061 ggctctcatg gacgatcttg ttaaaaaggt ggttgaatct cccgcacgtg ccgtagatga
     3121 ttttgtggca gttgccaatt ctccccgtta caaaaaaccg atccgtatcg cgaagaaata
     3181 ttggggtgaa ctgggcccag gcttaactac tggtgcctct gacgacgacc cttctggtat
     3241 tgctacctac tcacaggcag gagccatttt tgggttctcc tttctttgga tgtctctcta
     3301 tctgatacct tttatggttg tcgcccaaga aatgtgtggg agaattgctc atgttactgg
     3361 tcgcggaata tcgggggtgc tcaagcagca ctattcaaaa cgtatcgtac gaagtacggt
     3421 tctgatgctc tttgcggcga ataccctcaa cattggggca aatatagccg ctatggccgc
     3481 atcgactcgg cttgtgtttc ctgggttgcc atttactgtg ttggctgttt tcttcacgct
     3541 cctttctctt attttgcaaa ttagcatgcc ttattctcgg tatgagaagt ttctgaaata
     3601 tctgacgctt gttctttttg cgtatatcat caccgccttt cttatccgtg ctgattgggc
     3661 cgaggtgata cgtcatacgg ttcagccgca cttcatattt accaaggacg gtatctttat
     3721 gatgaccgcg attgttggga caactatttc gccttacctg ttttactggc aggcttcgca
     3781 ggttattgaa cagcaacgag aggaatatgg gcggaaaacg cttacttcac tcgccggcac
     3841 gaacgctaaa gaaatcagtt tgatgcgtaa agacgttacc accggaatgg ttttctctaa
     3901 tattgtcatg ttctttatta ttgctgtggc gggtactgtc ctcatgccaa agggaattac
     3961 tattgatact gcggcagatg ctgcccaagc cttgcgtcca cttgcagggg atttcgcgtt
     4021 cttactcttt gctatcggaa ttgtgggtac gggtatgctc gcgattcccg tcttggccgg
     4081 ctcggcgtcg tacgcttttt ctgaggcatt ttcgctcaat catggtctcg ataaacagct
     4141 caaacgggcg tatggctttt atggcgtaat tatactttcg atgggaatcg gcctgttgat
     4201 taactttttg ggaatccacc ccattaaggc ccttatttac tcggctgtta ttaatggcct
     4261 cgtggctcct gttctcttaa cgctcatact tctcgttgct aataaccaca agattatggg
     4321 tgaatggaaa aatggcccgg ttagtaatat tcttgggtgg tttattacta ttattatgta
     4381 cattgcaggt attgcaacac tctggggaat gttcttttaa gtattgatta ttttaagttt
     4441 tcatgatagg ataagggcgc cttaaaacag tatgcagccg cacggctgtg gagaaaaatc
     4501 atgccttcgt gggtgttcgg tgtaggggcg gtagccttgc tgctgggctt ggtttgtatc
     4561 tgtatgtcgc agcgagccct gcggatattt ggccagggag tgttggtgat cgctgcatat
     4621 tggatggctg tgatctttat tacagagttg cccctcattc ttcttgacct tattgtcacg
     4681 ggggttaaga tggcgggaac ggctggtatc gactggctaa aaggctttgt gggcatcaag
     4741 ccccaaggcg taaatgcggc ggagttggtc ttcgtctttc tcaaaccttc gttggttctc
     4801 tttctcctcg cgcgggcagg cttacgcttc gcgcagatca ttgagtcagc gattgtggaa
     4861 gatcagacgg tactccaaac cctccgggaa tcccgtcata ttcgctggta ctaaccaacc
     4921 agctctatcc ctgataaaca aaaagtgcct gccccggaag gggcaggcct ttgcatttta
     4981 gggcgagacg gcaaatgact acgtcgtctc gtcatccaga cactgttcca ttgcctggac
     5041 gagctggttg atatcccaac ctcccccctc gagagggaag agctctttga gccggtccca
     5101 gaggtccgga ccggtgagag gggtggcagg atcgtcgctc gtcgcgagga cgagcagctt
     5161 gagagtcgtg gcttccccgg ctgctttgaa ttttggcagc cgggtgagat gcatgatggc
     5221 attcatgata ccgatgttgc cacccatgat ctgctgtgct gttacgttcg ggacttctga
     5281 aagcgacatg actactcctt ggtgaggtaa acgagaaaaa tatactacaa aaaatatact
     5341 ttgtcaatat attggcgtat aaaaagaaaa accgtcgcac gagctgccgg atggcaacaa
     5401 gcgtgacggt taggctaagc cctaacgggc ggcttactta tgtagggcac gggagagtcc
     5461 cacgagaaac gcaattgtgt aaatcacgct acccgtaaag actccccatt gccgatttct
     5521 ccaactggtg taaaaccaga agggttgggt gacgaggctc aggacgaacc cgatccagtg
     5581 gccgctggtg atgaacatca tcccgattgc tcctccgagg atagcgatct gagaaatatg
     5641 atccatcacg gttggtttcg ttctttcccc ttttttgctc attctctcta actcccgtga
     5701 gtacaggagt atgctggatg gctatcgaga gggatgaggt atgtacgagc gaacgccgtg
     5761 gcctgctcag gccggccaaa gtcatgctca ccatacgcct cgtagccgca gatgatgccg
     5821 aactccggct tgattaggca ctcactgcct tccgggtaat cctcggcgta gaggcatgag
     5881 cacagtctcc actttgtccg gtcgaagtag agccggacgt tcccacctat cacgagaatc
     5941 catacacctt gggtcaccgt ccgtcggtac gttatgtgtt taatgtgcct tcggttacac
     6001 cactgaatca aatgtccccg attcatctcc ttgtactcct ttgaagcgca tattgtaaac
     6061 tatactatat ttcttgcctt acgacaatta ttttctctgt ggatgcatct tcagaggatg
     6121 gttgagagaa cacttctata taaaaac
//
LOCUS       DASWYB010000086         6792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_99242, whole genome shotgun sequence.
ACCESSION   DASWYB010000086 DASWYB010000000
VERSION     DASWYB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99242"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..900
                     /locus_tag="VGE59_04835"
     CDS             217..900
                     /locus_tag="VGE59_04835"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074983.1"
                     /translation="MTIQDWSTTTVDALRDGWDRIVTYLPDLLGAIVIIVIGVLVASL
                     VKWIVVRILEAARVQRAFDDLHLAKTLREANMTTNIAEIAGEFLKWVTIILFLIPAAT
                     VLGLPQVSNMLNDIIRYLPNVGSAVIILFLGALFAEFIGNVVRATAASLGSRMSGGLA
                     VFSRYVIYVFIGLAALTQLGIAIQVINILLTGFVAALAIAAGLAFGLGGKDAAADLIA
                     KIRRDFSSK"
     gene            924..1361
                     /locus_tag="VGE59_04840"
     CDS             924..1361
                     /locus_tag="VGE59_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074984.1"
                     /translation="MPRSHLLSLIARLAIGFVILGLLAFFLFTKLRKPSDKTITPDDT
                     VVIVTPTPVVSESPRPTDTNEDTATTTDNDVVVTKDKDGAVTITINDKGSKTVVTKGT
                     NSMESKSSAAGSQSTATARGRVTSASTSAGPGYASASASASSW"
     gene            complement(1429..2127)
                     /gene="rnc"
                     /locus_tag="VGE59_04845"
     CDS             complement(1429..2127)
                     /gene="rnc"
                     /locus_tag="VGE59_04845"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY1074985.1"
                     /translation="MLTEQHIAEIEAIIGLAFNDKNLLKQVFIHRSYLNENPGCELGH
                     NERLEFLGDAVLELVVTEFLYRRFPNPEGELTNWRSALVRGQHLAEISGELQLGDYLL
                     LSRGEAKSGGKTRQILLANMFEALLGAIYMDAGLEPARAFIEKHVTGRLEDILERELH
                     IDPKSKLQEITQARVGITPHYEVLREEGPDHAKEFTVGVFLATRKLAEAKGASKQSAE
                     QAAASVAIKQFSQE"
     gene            complement(2143..2613)
                     /gene="nusB"
                     /locus_tag="VGE59_04850"
     CDS             complement(2143..2613)
                     /gene="nusB"
                     /locus_tag="VGE59_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY1074986.1"
                     /translation="MNRHLARTLAMQTIYEWDFQAHKSIHEMLASNVNAVDHADDDTV
                     FVEKLINGVVANKEEIDGLITTAAPEWPLDQISVVDKSILRLAIYEILHETDVPAKVA
                     INEAVELAKTFGGDNSSRFVNGVLGTIYRQTERFKQDSANQPATGVAETTEDKE"
     gene            complement(2635..2820)
                     /gene="rpmF"
                     /locus_tag="VGE59_04855"
     CDS             complement(2635..2820)
                     /gene="rpmF"
                     /locus_tag="VGE59_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY1074987.1"
                     /translation="MAEPKKKLSATRSGRRRSHLALTVQAPAQCTNCKALVTPHTVCV
                     SCGFYKGKQVLPKNQAK"
     gene            complement(2869..5670)
                     /locus_tag="VGE59_04860"
     CDS             complement(2869..5670)
                     /locus_tag="VGE59_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1074988.1"
                     /translation="MEQKYNPHEIEAKWRSIWPKERDSYKLQPGDEPYYVLDMISYPS
                     SEGLHMGHWRPYTIADVWARYNTLLGKKVLSPVGFDAFGLPAENAAIKNGRHPADYTV
                     QSMGNFTRQIQEMGKMYDWSTLINTSSPEYYKWTQWIFTQLYKNGLAYKKAAAVWWCP
                     SCQTVLANEQVISGECERCGSEVIRKNLSQWFFKITDFADDLLSFEGLNWPAQVKTLQ
                     TNWIGKSQGAEIDFKVVNSEDVIRVFTTRPDTLMGVTYIVVAPEHTLVTALMTDETKG
                     KVEAYVLDASKKSDIDRMADDRTKTGVFTGSYVIHPITNAHIPVWVADYVVGTYGTGA
                     VMAVPAHDQRDYEFAQVYDLPIQTVIEPVVGTPQEHPEYRQSIVALVHDPKQDRYLTI
                     NWGEMGGTLLIGGGREEGEDPVACAQREIEEETGYSNVAFINKTETIHHSYFAHSKNV
                     ARAIDAVGVSFELASDATTATRLEAAEQGKFTVEWLTADEADKRIIDPLHRCVFERFA
                     KGNVYTGYGVLVNSGEFDGLTSEVAKDKIVAHLESQGRGRLTTQYRLRDWLISRQRYW
                     GAPIPVVTCEKCGEQVVPDDQLPVVLPKDVEFLPTGGSPLERHPSFKHTTCPTCGGDA
                     IRETDTLDTFVDSSWYFLRYTDPKNPSAPFSLEGVKAWLPVDFYVGGTEHSILHLLYA
                     RFVMKALHRLGHVPYHEPFQTFFGNGMVYLNGKKMSKSKGNIVNPDDMVKRYGVDAMR
                     GYILFMGPADQDVEWQENGITGVSRFLDKAWQSFAKVDSSNNATSLSPAIQKAYQDVN
                     AHLQRFTFNRCISSLMIALNEVNGHVNKAEAEVLIRLFAPFFPHFAEEVWSNLGHTSS
                     IFEATWPEVKIEASQTASYVVQVNGKLRATLDVLATAEENEVIALASELTAVKSHLEG
                     KSIEKTIFVAGRIINFVVR"
     gene            complement(5780..6688)
                     /locus_tag="VGE59_04865"
     CDS             complement(5780..6688)
                     /locus_tag="VGE59_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074989.1"
                     /translation="MNKKKMVMGAALFLLGVLMLLYAIFGYGEKTVPPSSPVTAPSSP
                     SQSAVEPTPPPGYITTETVEMDTRRKKVFASYSEAFRNLAARSSNPEVKAVYNFLVQH
                     EMAATLRGNSLSLFEKKKPNRVAVLTVLPEDPAEVQSLLREEAGARFVDETQMVCLRE
                     KLVSKFSAVLAYGHEWYHAYDYDKRGYSWPEGYYESGEGELNAYNFQLLLQSEVGGSA
                     YEAWVNRKCESVADEAQLHQGEAGYVLKAGDYDPELDKILGPSVSDFDRETRQKQCWR
                     AAVWRMYDRYLVGDPKQAKLDFIRDR"
BASE COUNT         1764 a         1724 c         1508 g         1796 t
ORIGIN      
        1 gcctcttttt agatttacac agagactatt ctaattctct aaaaataatc ttcttagacg
       61 tccgtcgttt gtgcatttat accagttgac aggggagaaa gagaaggtat atgatccagg
      121 taaggtcgaa aagccaacta gggcttctca caaacctagt agaaatacct atttatgaat
      181 gtaccgccaa taagcggcta ctgaaaggag ctttcaatga ctattcagga ttggtcgaca
      241 acaactgtcg atgcgcttcg tgatggatgg gatagaattg taacgtatct acccgattta
      301 ctcggtgcta ttgtaattat tgttattggc gtactcgtgg ccagcttggt aaagtggatc
      361 gtagtccgca ttcttgaagc ggctcgtgta cagcgcgcgt ttgacgacct tcaccttgca
      421 aaaacattgc gtgaagcaaa catgacaacc aatattgctg aaatcgcagg tgagttcttg
      481 aagtgggtaa ctatcatcct cttcctcatt cctgctgcga cagtgcttgg attgccacaa
      541 gtcagcaata tgttaaatga tattattcgt tacttaccaa acgtgggtag tgctgttatc
      601 attcttttcc tcggtgcgct ctttgctgaa ttcattggta atgtcgtccg cgctactgct
      661 gccagccttg gctcacgtat gtcaggtgga cttgctgtat ttagccgcta cgttatctac
      721 gtctttatcg gacttgctgc ccttacccag ctcggaattg ctatccaagt cattaacatc
      781 ttgttgactg gcttcgtagc tgccctcgct attgcggcag gcctggcatt tggccttggt
      841 ggcaaagatg ctgcggcaga ccttatcgct aagatccgac gtgattttag ctcaaaataa
      901 cttttaggag atattgcttc gttatgcctc gatcacactt gctcagtctt atcgcccgtc
      961 ttgccatcgg ctttgttatc cttggcctct tggcattctt tctcttcacc aaacttcgta
     1021 aaccttcgga taaaacgatc acaccagacg atacggttgt tattgtaacg ccaacgcctg
     1081 tcgtaagcga atcaccccgt ccgacagata cgaatgaaga tactgcgaca acaacagaca
     1141 atgacgttgt cgtcacgaaa gacaaagatg gagccgttac gattactatc aacgacaaag
     1201 ggagcaagac ggtcgtaaca aaaggcacaa actcaatgga aagcaaatct tcggcagctg
     1261 gctcacagag cacggcgact gctcgtggac gagtgacatc ggcttccaca tctgctggcc
     1321 ctggctacgc ttcagcatct gcctctgcta gctcgtggta aaacgatcct cggcacacaa
     1381 aagcccggag caagactccg ggcttttggt atccaggcgg actaattatt actcttggct
     1441 aaactgttta atcgcaacag aagctgcagc ttgttcggct gactgttttg aagcaccttt
     1501 ggcctctgcc aatttacggg tcgcaaggaa aactccaaca gtaaattctt tggcgtgatc
     1561 ggggccttct tcgcgcaaca cttcatagtg aggggtaatc ccaactcgag cctgcgtaat
     1621 ttcctgaagt ttactctttg gatcaatatg cagttcgcgt tcgagaatat cttcgaggcg
     1681 gcctgtcacg tgcttttcaa tgaacgcacg tgccggctcg agaccggcat ccatataaat
     1741 agccccgagc aaggcttcaa acatatttgc taagagaatc tgacgggttt taccacctga
     1801 ttttgcttcg ccgcgtgaga gaagaaggta atcacctaat tgcaattccc cggagatttc
     1861 ggctaaatgc tgtccacgca caagggcact acgccaattg gtgagctctc cctcgggatt
     1921 aggaaaacga cggtagagaa attctgttac gacaagttct aaaacggcgt cgcctaaaaa
     1981 ttctagacgt tcgttatgcc ccaactcaca gccaggattt tcgtttaaat acgatcgatg
     2041 aataaagact tgtttgagca agtttttatc gttaaaagca aggccgataa tcgcttcgat
     2101 ttcggcgata tgctgttcag taagcatgtt gagtccttac ccttattctt tgtcttcggt
     2161 tgtttcagcc actccagtag ctggctgatt ggcactatct tgcttaaagc gctctgtttg
     2221 acggtatatc gtaccaagta cgccattcac gaagcgcgat gagttatcgc caccaaatgt
     2281 cttggcaagc tcaacagctt cgttaatcgc gaccttggct ggtacatcgg tttcatggag
     2341 gatttcataa atcgccaagc ggagaattga tttatcaacc acggaaattt gatcaagcgg
     2401 ccactcagga gcggcagtcg tgatcagccc atcaatttct tctttattcg caacaacacc
     2461 gttaatcagc ttctcgacga atacagtatc atcatcagca tgatcgacgg cgttgacgtt
     2521 agaagcaagc atttcatgaa tcgacttatg tgcctggaaa tcccattcat aaatggtttg
     2581 cattgccagt gtacgagcca gatgtcgatt cataggaatc ctttactttt aaacttactt
     2641 tgcctgattt ttaggaagaa cttgcttccc tttatagaaa ccacacgaaa cacaaactgt
     2701 atgaggagta acaagcgcct tacagttagt gcactgagct ggagcttgaa ccgtgagcgc
     2761 taagtggcta cgtcggcgac cagaacgtgt ggcggatagt ttctttttcg gctctgccat
     2821 agttatttcc tatgttttat agtagcataa tttgcctgaa tacaagagtt atcttaccac
     2881 aaagttgata attcgtccag caacaaatat agttttttcg atacttttac cctcaagatg
     2941 gcttttcacg gctgttagtt cacttgccaa ggcaatcact tcgttttcct ccgctgtagc
     3001 tagcacatca agtgtagcgc gaagttttcc gttaacctgg acaacgtacg aagccgtttg
     3061 agaagcctca attttcactt ctggccatgt agcctcaaaa attgaacttg tatgccccag
     3121 gttgctccag acttcttcgg cgaaatgtgg aaagaaagga gcaaaaagac gaataagtac
     3181 ttcggcctcg gctttattta cgtgaccgtt tacctcattt aaggcaatca tcaagctgct
     3241 aatacatcgg ttaaaggtga atctctgcaa atgagcattc acatcctgat aggctttttg
     3301 aatagccgga gagagagatg tagcgttatt tgaactatca actttggcaa atgactgcca
     3361 ggctttatca aggaaccgac tcacaccagt aataccgttt tcttgccatt ccacatcctg
     3421 atcagctggt cccataaaga gaatgtagcc gcgcatagca tcaacaccgt atcgcttcac
     3481 catatcatcg gggttaacaa tatttccctt tgatttagac attttcttac catttaagta
     3541 gaccatgcca tttccaaaga aggtctggaa tggttcgtga taggggacat gtcctaatcg
     3601 gtgtaaagcc ttcattacaa accgagcata caaaaggtgg agtatggaat gctctgtgcc
     3661 gccgacgtaa aaatcgacag gcaaccaagc ttttacacct tccaaggaga atggagcgga
     3721 cggattctta ggatcagtat aacgcaagaa ataccacgaa gaatcaacaa aggtatctaa
     3781 cgtgtcggtc tcacgaatag catctccgcc acatgtaggg caggtcgtat gtttaaagct
     3841 cggatggcgc tccagcggcg acccaccggt tggcaggaat tcgacatcct tgggcaagac
     3901 gacaggtaat tggtcatcag gaacaacttg ttcaccacat ttttcacagg taacaacagg
     3961 gatcggcgct ccccaatatc gctgacgcga aataagccag tcgcgcaaac gatattgcgt
     4021 cgtcagtctt cctcgtccct gggattctaa atgggcaact attttatctt ttgctacttc
     4081 gctggttaaa ccgtcaaatt cccctgaatt aacgagtact ccataccctg tgtaaacatt
     4141 ccctttggca aaccgttcaa agacacatcg atgcaaggga tcaataatac gtttatcagc
     4201 ctcatcggcg gtaagccact caacggtgaa tttaccctgt tccgctgctt ctaatcgagt
     4261 agcggtcgtc gcatccgatg ctaactcaaa cgaaacgcca actgcatcga tggcgcgagc
     4321 aacatttttt gaatgtgcaa aatagctgtg atggatcgtc tcggttttat taataaacgc
     4381 aacattcgaa taaccagttt cttcttcgat ttctcgttgc gcgcatgcga caggatcttc
     4441 gccttcttca cgccccccgc cgataagcag agttccgccc atctcccccc agttaattgt
     4501 taaataccga tcctgcttgg ggtcatgcac gagcgccaca atgctctggc gatactcagg
     4561 atgttcttgg ggtgtcccca caaccggctc gataacagtt tgaatgggta agtcatacac
     4621 ttgagcaaac tcgtaatcac gttgatcatg agccggaacg gccataacgg cgcccgtgcc
     4681 atacgttcct accacatagt ccgcaaccca taccggaata tgggcattgg taatcgggtg
     4741 aataacatac gagccagtaa acactcccgt tttcgtacgg tcgtcagcca tacggtcaat
     4801 atctgacttc ttcgaggcat cgaggacgta cgcttcaacc ttaccttttg tttcgtccgt
     4861 catgagagca gtaaccaatg tgtgctctgg ggctacaaca atgtaagtta cccccatgag
     4921 cgtatcggga cgggtggtaa atacacgaat gacatcctca gaattgacta ctttaaagtc
     4981 aatttcagcg ccttggctct ttccaatcca gttggtttgt aaagttttaa cctgagccgg
     5041 ccagttgaga ccctcaaaac ttaaaaggtc atcagcaaaa tcagtaatct taaagaacca
     5101 ctgacttaaa ttttttcgaa taacttcact gccacagcgt tcacactcac cactaataac
     5161 ttgttcgttt gccaaaactg tttggcagct tgggcaccac catactgcgg cggctttttt
     5221 ataagccaaa ccgtttttat ataactgcgt aaaaatccac tgcgtccatt tgtagtattc
     5281 cggcgacgat gtattaatca gcgtggacca gtcgtacatt tttcccattt cttggatctg
     5341 acgcgtaaag ttccccattg actggaccgt ataatcagcc ggatgacggc catttttaat
     5401 ggcagcattc tctgccggta agccaaatgc atcaaaacca acgggggaga gtaccttttt
     5461 tccaagcagc gtattatagc gagcccatac atcggcaatc gtgtaaggac gccaatgtcc
     5521 catatgtaaa ccctcgctcg agggatagga aatcatatct aaaacatagt acggctcatc
     5581 gccgggctgg agcttataac tatcgcgttc tttgggccaa atcgagcgcc actttgcttc
     5641 aatttcgtga ggattatact tctgttccat atggttcaat tttacgcttc accagaagaa
     5701 aatgcaatga aaaagaaaag ccgcagacca agacacgcat gtctcaaacc tgcagcttct
     5761 aaggtcggcc aactgagctc taacgatccc ggatgaaatc cagcttggcc tgtttcggat
     5821 ctccaacaag ataacggtca tacatccgcc acactgcggc acgccaacac tgcttctgtc
     5881 gggtctcccg gtcgaagtcg gaaacggaag gcccaagaat cttgtccaac tcgggatcgt
     5941 aatcaccggc cttaagaacg tagcctgctt ctccttggtg gagctgcgct tcgtcggcga
     6001 cagactcaca cttacggttt acccacgcct cataggccga tccgcccact tccgactgaa
     6061 ggagaagctg aaagttgtac gcattcaact caccctcacc ggattcgtag tagccttccg
     6121 gccaagaata accacgtttg tcgtagtcgt aggcgtggta ccactcgtgt ccgtacgcaa
     6181 gcaccgcaga gaacttgctg actagtttct cacgcaggca caccatctgc gtctcgtcga
     6241 cgaaacgagc acccgcctcc tctcggagga gcgattgaac ttcagccgga tcctctggca
     6301 agaccgtcag gactgccacc cggttcggct tctttttctc aaagagactc agactgttac
     6361 cacgaagcgt cgctgccatc tcatgctgga cgaggaagtt gtagaccgcc ttgacttcgg
     6421 ggtttgaact tcgtgcagct agattacgaa acgcttcaga gtaactggca aagacctttt
     6481 tccggcgcgt atccatctcc accgtttcgg tcgtaatgta tcccggcggc ggagtcggct
     6541 ccaccgctga ctgcgatggc gacgaaggcg ccgtaacagg ggaggatgga ggcaccgtct
     6601 tttccccata tccgaaaatc gcgtagagaa gcatgagcac gccgagcaaa aacagggcag
     6661 cccccatcac catcttcttc ttgttcactc tcttccccct tagaacgaag ccactaccag
     6721 gatcaacgga tcgttaatcc tggggtgggg gcgtctctac cgagacggcc gaatctaacc
     6781 tccaacaaca ca
//
LOCUS       DASWYB010000087        18142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_916272, whole genome shotgun sequence.
ACCESSION   DASWYB010000087 DASWYB010000000
VERSION     DASWYB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18142
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916272"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VGE59_04870"
     CDS             complement(<1..382)
                     /locus_tag="VGE59_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEY1074990.1"
                     /translation="MKKRYVASPILLLTVATVWNVVFKADTPKATAEDIQNITAPQIA
                     DSRHPIEVFQVGVGSGETENDVLAQLSTVVYPEDKVRFLIDPKFGLGTVVHIERALPV
                     TIKDGKRVITVRTWDNTVGELLDSV"
     gene            complement(1243..3147)
                     /locus_tag="VGE59_04875"
     CDS             complement(1243..3147)
                     /locus_tag="VGE59_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY1074991.1"
                     /translation="MTELDQIQQSPLFEGLNSEQKQAVITTEGPVLVLAGAGSGKTKT
                     LTHRIAYLLREKKLSPFNVLAVTFTNKAAGEMAERISKLLSGNENNRINLPFLGTFHS
                     IGVKLLRRDGHHIGLDPSFTIYDSDDQLSLIRSICQELAISPKQYSPQAIRSYISGAK
                     NELMTAEQYRKFAHGYFQTLVCDVFARYEKALTSAQAVDFDDLIGLPVKLFSEHPEVL
                     ERYQDLWRYIMIDEYQDTNKAQYEFVRLLAVKYRNIFVVGDDAQSIYGWRGANFRNIL
                     DFEKDYPDAAVIKLEQNYRSTQIILDASNAVIEHNTQRINKALWTEKGEGAPITVYQA
                     EDGRDEAEFIMTEVISLQRQGYRLGDMAILYRTNAQSRALEEICLNYTLPYRLVGAVR
                     FYERKEVKDILAYLRFICNSKDEVSFERLLSVPPRGLGDKSFQDLKKVVIHGEPESTL
                     SPRIMKAWKPLELHFDRWRKLRDTISVTDLIDIVVKESGYEKFLLDGTPEGEARFENV
                     KELKSVASRTEDLPTFLAEVALVSDVDGYNPDEDAITLMTLHSAKGLEFPVVFMVGME
                     EGLFPNLRSVMDPLELEEERRLCYVGITRAKDKLYCLHAASRLIYGSFQANLPSRFIN
                     EIPEHLVDKI"
     gene            complement(3198..4568)
                     /gene="dnaB"
                     /locus_tag="VGE59_04880"
     CDS             complement(3198..4568)
                     /gene="dnaB"
                     /locus_tag="VGE59_04880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY1074992.1"
                     /translation="MARRETASSLTNSLQSLATRMPPQNVEAEQSILGAVLIDADSMI
                     RITDKLKPEDFYREDHKVIFEHMLKLFEKRQPIDILTLTDSLDSSKQLAFVGGAGYIA
                     SLTSMVNSSAHIVSHAEIVRSKALLRRLIQAGGKIVDLGYQEDAESDMILSDAEAALF
                     NVSNAYLSSNFVPIGNILSESFDRIDELHRNKGAVRGVPTGFKDLDSLLGGLQRSDLV
                     ILAARPSMGKTAFVLNIAENAAVKSGAKVAVFSLEQSKDQLVDRLLCSQAQVDSWKLR
                     SGNLSDEDFPRIGAAMGVLSEAPIFIDDTPGLTSLEIRTKSRRLQMEHGLDMIIIDYL
                     QLMQGRSGIENRTQEISEISRSLKGIARELNVPVIALSQLSRAVEQRPDKRPMLSDLR
                     ESGSIEQDADVVMFIYRDEYYDRESEKKNIAEILVRKHRNGPVGEVDLYFQGEQTRFR
                     NLDKRH"
     gene            complement(4571..6106)
                     /gene="dnaX"
                     /locus_tag="VGE59_04885"
     CDS             complement(4571..6106)
                     /gene="dnaX"
                     /locus_tag="VGE59_04885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY1074993.1"
                     /translation="MATQISLYRKWRPQTFEDLKGQDVIRDTLRYALTHNQMSQAYLF
                     CGPRGTGKTTTARLIAKLVNCLAEGEEKPCNTCSNCLSITDGANLDIIEVDAASNRGI
                     DEIRQLRDSVRFAPTQARYKVFIIDEVHMLTREAFNALLKTLEEPPSHALFILATTEA
                     HKIPATIISRCQRYDFRLATLDGLTTHLMYVAEQEGMNLAPDAARFIARLGDGSFRDS
                     LSLLDQVRAANHPEYTLQVVEEMFGYIPVEQMATCLTAILSGDLVKAHGSVDAALARG
                     ADLRAFCDQLLALSQQCLEALVLQEFKTLPSALGTAVQGAGLSRLVQWLELLLEAIGD
                     SKLSPIPRLPLDVAITKLNVAPQAARAVQPMAEVRVATPPAIAAGPSITAPPPRPQEE
                     SVVVPMQETVSVPVMVAVPHVVEAQDWQRVLEGLKKDAPSLLTSLSGARVTGVENGTM
                     NVAVKYKMHADKVNQVKNKMKIEVVLQEVFQVPLKIEAVVVKDMATEDDSVDVASVFE
                     FEE"
     gene            complement(6159..7610)
                     /locus_tag="VGE59_04890"
     CDS             complement(6159..7610)
                     /locus_tag="VGE59_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY1074994.1"
                     /translation="MRKIARILSFFIILGWIGTSFAGTNDGLAFLRSKQGPDGTISGL
                     GNTGWAVMALSGYGERNDRAIQSLQTVQTSLSTRSATDIEKQILALVAGGENPRTFAG
                     VDAVVELQSRVMGNQIGDTDYLNDDIFGLLALRSAGASVPAEVSSQLLQHQNQDGGWG
                     LIVGGASSTDLTAVALIALKGSVSQESLVRAMDYIRSLQNDDGGFAISSGGSSVASTA
                     WVDWMLTEYGQSYAPWSKGGHSPRDFLLSTQGSDGSWGGSVLMTSYALIALSGRGFPI
                     IGQFSTPTPTPSATTTPTPSPTLSVAPSPTPSPSHSPSTTPTVLPTATPSPSPTVTFS
                     PLPTSVPSPSPSVSAIPTPSASSIVVPTASPTLNPSPGLLSTPAPTVLPVVAPTSTLM
                     PVSTAPKVMRRTISLVQRIFPAKGTSAPVVTPTVSLVPVIFSPVPSDMSNGQFVPLNL
                     HDYSRHFFFGGLLLAVNTLATGYEIWQAHKRTK"
     gene            complement(7637..8146)
                     /locus_tag="VGE59_04895"
     CDS             complement(7637..8146)
                     /locus_tag="VGE59_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074995.1"
                     /translation="MQFSFRRSDTPGVARSSRQTRVARRPPPKISVMVYFAQAGKSKK
                     VGSFKAVPGAKLGESLRQAGVTCAHEHPEYPDDFITAVNYRRWRIVASHDPVTRTYVW
                     GVGSQQITFKVNGDEFINVGAEWYELPNRSCRLVVELEDVIDMEEPDGVSLRRLTTPL
                     PGSIVFGGA"
     gene            complement(8213..8893)
                     /locus_tag="VGE59_04900"
     CDS             complement(8213..8893)
                     /locus_tag="VGE59_04900"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEY1074996.1"
                     /translation="MDPGPWRGFFMSLGVSRVISGLLMLATCVFGTTLVRDVQGAEVK
                     SVPNVSPHVERTFESTPLVNTPAGVGNSVRREQTVIAPPTRRPRMKVTPEVATSQVVR
                     TAETIAPTVIITPSPNPVVTVTPQLREVGYSIMGGGVNLEGKIETQEEPVGLLTKRIA
                     ANRGISFIYEVSDMGWFVTEIAGVKQNPRAGYYWLFYVNGALANRSADQVLVKAGDTL
                     TWRYEKTN"
     regulatory      complement(8931..9075)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9217..9375
                     /locus_tag="VGE59_04905"
     CDS             9217..9375
                     /locus_tag="VGE59_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074997.1"
                     /translation="MDHETEMPKTDMPVEGEEMETTTPEGDTEEGEEEMPAKDGEGDA
                     EDTEEGEE"
     gene            complement(9487..9768)
                     /locus_tag="VGE59_04910"
     CDS             complement(9487..9768)
                     /locus_tag="VGE59_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074998.1"
                     /translation="MSELDERPIMIFLDCGNPECETKWMAAGTYEGVEGSVPRVMDER
                     DRICPSSGCRCSDSIVREWGPLKFELMGKIWVVAGQPWIWTPAGYNPAR"
     gene            complement(9896..10477)
                     /locus_tag="VGE59_04915"
     CDS             complement(9896..10477)
                     /locus_tag="VGE59_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1074999.1"
                     /translation="MKVLFVTENGRVSHAGLDGVEINIFEKATDQFQRYPHTVVRTVA
                     DRLGFTVQGIEESARVKRENPFQWLDLRYGNGLEILTPSSESGRVVHFWKVAGEMDED
                     EKLRRGIPVSGRFPASFLQKRPPEGGWVDPYVYLLDPSGLTPVERDLIGLFLPGPCYS
                     GGTRFDGYFQMHIRGVAEVSRRLGFVPQFEGKE"
     gene            complement(10619..10936)
                     /locus_tag="VGE59_04920"
     CDS             complement(10619..10936)
                     /locus_tag="VGE59_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075000.1"
                     /translation="MKTVITIEIRTEVDVDTNLTNLFDNHGLDNFIYNTGTSVVEQVW
                     KMVAQQVGEQHREVVEDVKKYWVAKDARRNLLRSGEDAHERSKELGETMKQIEEKYGL
                     ESK"
     gene            complement(11031..11372)
                     /locus_tag="VGE59_04925"
     CDS             complement(11031..11372)
                     /locus_tag="VGE59_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075001.1"
                     /translation="MIVRFGKGAYLEVSNEKGNVTYYQLQGEMEVELWGNPPMRLADL
                     TGPRQSGGRVHATRLSGCTSFGDESTRVEIDDITLQGDTETLSGRRGGYYGTLYHVKS
                     GAESYNFFTGS"
     gene            11673..12425
                     /locus_tag="VGE59_04930"
     CDS             11673..12425
                     /locus_tag="VGE59_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1075002.1"
                     /translation="MQPIIHELGLSPKEEVVLIALIELGAAQVTRIAQKANLNRTTCY
                     DILEHLNILGLVGYVADQKKKTFVAEPPETLVSYLERRSKQFAAKAEEVRKALPELKA
                     KYSERGRGPKIRFYEGKEGLKTVYEDTLTTHEPIRAYASVRNMHDALPDYFPEYYKRR
                     TKKNISIRAILPATPEGIERSQHDAEEARESALVDPEQFNFSPEINVYDNKIAIMSLA
                     EEFAVIIESKEIAEAQKKIFELAWKGAKSEEK"
     gene            complement(12525..13184)
                     /locus_tag="VGE59_04935"
     CDS             complement(12525..13184)
                     /locus_tag="VGE59_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075003.1"
                     /translation="MNQIALALQLRRFAYTFAAREIAPNVVADGIGWNVTINATTGIS
                     ISVGFNGFYTDYRYRIEAEALGKSLENSEDQILRNLKCEEYGNHVDGSHKDGYKVWLW
                     SHYKVSLDLIPEDIRDSIVDCTRRGSEANRAYHLLDDTRRVSVYELDEEGTSRLIGVV
                     REAGAIHKAARLKELDADKRECIGRVLQFLGLDGLGVVERGQRDKLSPKPLEWVEVSC
                     Y"
     gene            13360..14151
                     /locus_tag="VGE59_04940"
     CDS             13360..14151
                     /locus_tag="VGE59_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1075004.1"
                     /translation="MEQQLTQLGLSPKAARTYLALLELGPSSILAIARKAGLNRTTLY
                     DIIPVLITEGLISRVINSKTEKYEAESPTRLPLLLERKIQELQLQMGTAKDLATKLGY
                     LAFTGKESKPKVTLYEGEEGIKKMYEDSLLCKTYIRSFLAPEELKSFDDEFADSYFER
                     RRKKNIRIQGIINDSPESRNYIEHEKELLRDIHLVPTNAMNIKPEVYIYDNKVAFYSL
                     KEKYGVLIESADIAEAIAKLYDLAWAESERSDKELRIQKAPNSKS"
     gene            14342..14809
                     /locus_tag="VGE59_04945"
     CDS             14342..14809
                     /locus_tag="VGE59_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075005.1"
                     /translation="MFENKSLSYFGTCLDTLLPYALSLLVFAGFIHRAIGHMPRAKPF
                     VQTARILRILSLFMVGLVVTPYSVGPEIKLAHTIVAMVASDIMLGLTFWLALFVRRDR
                     VSVSLLITQVTAGILAGLALLDKGGLFLAGEMVYVLAFGAILARTIHLTHPAP"
     gene            complement(14847..15587)
                     /locus_tag="VGE59_04950"
     CDS             complement(14847..15587)
                     /locus_tag="VGE59_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1075006.1"
                     /translation="MDYAKLLTFIGFSETEAKLYLANLRLGPATAIQLSQKVGISRQM
                     VYTLLPSLLEQGLIKETVVGSRRLFEALSPEVLQDRAADILTEVKKAVPVLKTKQATN
                     NALPILSVYENPISMREWYRRFMEEAAEGDELWIWATNKTWLSVDPDFLEQFVAFKQR
                     MGIKDRIIAPDTPESRLFSTKLGQSHAQYRFTRTWWHTATEKWIWNNTVSYLTISENA
                     TNLIVLESKQLTELERFNFQQVWNSLNP"
     gene            15781..16536
                     /locus_tag="VGE59_04955"
     CDS             15781..16536
                     /locus_tag="VGE59_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEY1075007.1"
                     /translation="MKDPHAPPTSLTIMQPPREPFFIFSADGIFYSLNGEGATIGYPA
                     VYARLHGCNLNCSWCDTPYTWNRSLTEFHEYLQRGRLSIPDTLEQLIAADVSGCKRLV
                     FTGGEPLLQQKAIAAILPQLTHWSVEVETNGTIAPQKELFDCQFNCSPKLPNSGISAE
                     RAVKPQAIHTLKGLNTYFKFVVRTANDVAEMQRIYEQPFQIPRDRIILSPEGITVSDT
                     VRVMQNLAEIAKDQGYRLTPRLHILLWGDTRRT"
     gene            16543..16923
                     /gene="queD"
                     /locus_tag="VGE59_04960"
     CDS             16543..16923
                     /gene="queD"
                     /locus_tag="VGE59_04960"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY1075008.1"
                     /translation="MSLQVSVTKIFTFESAHSLPSHRGKCRRPHGHSYKLEVTLSGPL
                     KARGSSQGMVMDFADVSKVVKREIITPWDHQSLNDLFSFPTTAENLALEAFRRLRAAK
                     LTVTRVRLWETATSYAEVESHDMG"
     gene            16910..17644
                     /locus_tag="VGE59_04965"
     CDS             16910..17644
                     /locus_tag="VGE59_04965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1075009.1"
                     /translation="MTWDNRIVAAYQASDEKPDKKYSIGPTVVGLADPEGKEVLDLGC
                     GSGFFTRRLIWAGAKRVIGLDNCPEKIRKAKIQPLPRLEYRLGDMFTDELPSVDVVTA
                     PFTLNYARNEDQLLLLLARLYASLRAGGRLVSAIDLPEGRDLIRFGARKTLLGPKQDG
                     TLIQIELFNQGLHFCTLPLAAHYFAPATIERTLTQIGFINLTWHKPIISPEGFKAFKN
                     APDFWKDYPDHPELGYITAQKPPSKS"
     gene            complement(17715..>18142)
                     /locus_tag="VGE59_04970"
     CDS             complement(17715..>18142)
                     /locus_tag="VGE59_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1075010.1"
                     /translation="AVRIIVIQRNAEQDQSVRTRVWSAIDAIPLTQRPMDIALWSAWK
                     ETDLPRGVGYEYFAIGRVPQYSRVYISEARAHVHQNSDSFIYVISGAGVCVLGKEEYA
                     IGEGNLIVVPRGLRHALRGSFVASSGQWGGEIDNDYTPK"
BASE COUNT         4842 a         5052 c         4003 g         4245 t
ORIGIN      
        1 tcaccgagtc taaaagttcg ccgacggtgt tgtcccaagt ccgaactgtt atcacccgct
       61 ttccatcttt aatcgtaact ggcaacgctc gttcaatatg cacaaccgtg cctaacccaa
      121 actttggatc aattaaaaaa cgcaccttgt cttcagggta aaccaccgtc gacaattgcg
      181 ctaagacatc attttctgtt tcgccacttc ctacgccgac ttgaaatacc tcaatcggat
      241 ggcgagaatc ggctatttgc ggcgcagtta tattctgaat atcttctgct gtcgccttgg
      301 gcgtatcggc cttaaataca acgttccata ctgttgcaac agtcagtaac aagataggcg
      361 aggcgacata acgctttttc atagtcaaga aataccctaa caggacagcc ccttttgcgc
      421 aagtgaatac caccgcccat accttgacaa atcaggaata tatgtatata ctacagtcgt
      481 attctgcaat acgcagaaga gcactctcgg ccaaccggcc cctgttcaca acggaataat
      541 gagaagaaca cagacgaggg acttttcgag gaatgcgctg ttccaaacgc tgcttaccag
      601 cggcaatcgg cgcactctct ccatcccgga ggtccggcca gcaacctgca cttgccccgg
      661 accgatctgt acggacatga tcgaggagca gacgtccctg ccaacttacc ggtggttcga
      721 acggacccag acgcatctga tggcggtcct gggcgtctgc ctctgggata cctggagctg
      781 gaaaaccaac ctccgggagc tcccgcatga ggcctggagc aacctggttc gctgggatgc
      841 tgatggtcac cccatttccg tctactggtc tcgcacgccg acagaaggag aaaagtggga
      901 gcctgagccc tcaaagctca agcgaacgac cgagctcatg cgactgatgc gagagaacca
      961 tgttaccgtc gtcggactcg ccgcggcaac gcggaggaat cccggatacg tggtcacggc
     1021 ccttcgaaac ggaccgaccg cctggttcta tgaaatcagc cgccggtatt tcgaagacgg
     1081 gtgtccggac tttagtccgc acacctgccc cgtgttcacg accggtgccc ggcctatgcc
     1141 ggagaacacc gcgccctact ggctgtccga atccgaatag gaatggatac gccacaccgg
     1201 tggaagtagt ctcagccgag actgcctcca ccggttttgt ttttatattt tatcgactaa
     1261 atgttcggga atttcgttaa taaatcgcga tggcaaatta gcctggaatg acccataaat
     1321 aaggcgtgat gcagcatgga ggcagtagag cttatctttg gcacgggtaa taccaacgta
     1381 acagagtcga cgctcttctt cgagttcaag gggatccatc acactgcgta agttaggaaa
     1441 gagcccttct tccatgccca ccataaagac aacgggaaac tccagacctt tggccgaatg
     1501 taacgtcatg agtgtgattg catcttcatc ggggttatag ccgtccacat ctgagacgag
     1561 agccacttct gctaaaaagg ttggtaagtc ctcggttcgt gacgcaactg atttcaactc
     1621 tttgacgttc tcaaaacgcg cttctccttc gggagtacca tccagtaaaa acttttcgta
     1681 accagattct ttaacaacta tatcgattaa atcggttact gaaatcgtgt cacgcaactt
     1741 acgccagcga tcaaagtgta attcgagcgg cttccaggct ttcattattc gaggagacag
     1801 agttgattcg ggctctccgt gaatcaccac ttttttaaga tcttgaaacg acttatcccc
     1861 taatcctcga ggcggcacac tgagtaaccg ctcaaagctc acttcatcct tagagttaca
     1921 aataaagcgc aagtaggcca aaatatcttt gacctcttta cgttcataga atcgaactgc
     1981 gccgactaac cggtatggca gggtgtaatt taaacaaatt tcttccagag cacgggactg
     2041 ggcattggta cgatacaaaa tagccatatc acccagacga tatccttgcc gctgcaggct
     2101 aataacctca gtcataataa actcagcctc atctcgccca tcttccgcct ggtaaacagt
     2161 aatgggtgcg ccttcgcctt tttcggtcca gagcgcttta ttaattcgct gcgtattgtg
     2221 ctcaatcacg gcattcgacg catcaaggat aatttgagtc gagcgatagt tttgctccag
     2281 cttaattacc gcggcgtccg gataatcttt ctcaaaatcc aaaatattac ggaagttcgc
     2341 cccgcgccaa ccgtaaattg actgcgcgtc gtcaccaacc acaaaaatat tacggtattt
     2401 tacggctaaa agacgaacaa attcgtactg cgccttgttc gtatcctgat actcatcaat
     2461 cataatgtaa cgccatagat cctggtaccg ttccagaacc tcaggatgct cgctaaagag
     2521 ttttaccggc aaaccaatga gatcatcaaa atccactgct tgcgctgacg taagggcctt
     2581 ctcgtagcgt gcaaagacat cgcagactaa cgtttgaaaa tagccgtgcg caaacttacg
     2641 atactgttca gcagtcataa gttcgttttt agcacctgaa atgtacgagc gaatcgcctg
     2701 aggagaatat tgctttgggc taatcgccaa ttcttggcaa atcgaacgga tcaaggagag
     2761 ctgatcgtct gaatcgtaaa tcgtaaaact ggggtcgagt ccaatatggt gaccatcccg
     2821 gcgaagcaac ttaactccga tcgagtggaa cgtacccaaa aacggcaaat taatccggtt
     2881 attttcgtta ccggacagta atttggaaat acgttcggcc atttcaccgg ctgctttatt
     2941 ggtaaaggta acagccagca cgttaaaagg cgaaagtttt ttctcgcgca acaggtaagc
     3001 gatacggtgc gtgagcgttt tagtctttcc actccccgct cctgccaaca ctaaaacagg
     3061 cccttccgtc gtaatcacgg cctgtttttg ttcgctattg agtccctcaa aaaggggtga
     3121 ctgctggatt tggtcgagtt ctgtcatgac tgtcctaccc taacaaaaaa tcctctcctt
     3181 ggcgagatac ggccacctta atgacgctta tccaggttac gaaaacgagt ttgctcaccc
     3241 tggaagtaga gatctacttc accgacagga ccattacgat gttttctgac taaaatttcc
     3301 gcaatattct ttttttcgga ttctcgatca tagtattcgt cacgataaat aaacatcaca
     3361 acgtcagcat cttgctcgat cgaacctgat tcacgcagat ctgaaagcat aggacgtttg
     3421 tcggggcgtt gctccacagc acgagaaagc tgcgaaagag caatcacagg aacgttcagc
     3481 tcacgcgcaa tgcccttcaa agaccgagaa atttctgaaa tttcttgggt acggttttcg
     3541 atcccgctgc gtccctgcat cagctggagg tagtcaataa tgatcatatc caagccgtgc
     3601 tccatctgga gacgacggct cttggtacgg atctcgagag aggttaagcc gggcgtgtcg
     3661 tcaatgaaaa taggggcttc cgaaagaaca cccatagctg ccccgatacg aggaaaatct
     3721 tcgtcactta aattaccact gcggagcttc cagctatcaa cttgggcctg agaacaaagc
     3781 aaacgatcga ccagctgatc tttagactgc tcgagggaga acaccgcgac ttttgcgcct
     3841 gattttacgg ccgcgttctc ggcaatgttt aagacaaacg ccgtctttcc catggaaggc
     3901 cgggcggcga gaataactaa gtccgatcgc tgcagaccac ctagcaggct atctaagtct
     3961 ttaaagccag tcggaacacc ccgaaccgct cctttattac ggtggagttc gtcgatacgg
     4021 tcgaacgact cagacaaaat attgccgatc ggcacaaagt tactgcttaa ataggcatta
     4081 ctgacgttaa aaagagccgc ttcggcatca ctcaggatca tgtcgctctc cgcgtcttct
     4141 tggtacccaa ggtccacaat cttgccgcca gcttggatta atcggcgcaa taacgctttg
     4201 ctccgtacaa tctccgcatg gcttacaata tgcgccgagg agttaaccat actcgttaac
     4261 gacgcaatgt atcccgctcc ccccacaaac gccagctgtt ttgacgaatc caagctatca
     4321 gtcaaagtta aaatgtcgat gggctgacgc ttctcgaaca acttgagcat atgttcaaag
     4381 ataactttat gatcttcgcg ataaaaatct tctggcttta gcttatccgt aatacggatc
     4441 atcgaatcag catcaattaa aaccgcacct aaaatactct gctcggcctc cacattctgt
     4501 ggtggcatac gagtagccag tgattgaagt gaattagtga gtgagcttgc agtttctcgt
     4561 cgagccatac ctactcctca aactcaaaga cagaggccac atcaaccgag tcatcctcag
     4621 ttgccatatc ttttaccacc acagcttcta tcttgagtgg tacctgaaat acttcttgaa
     4681 gaacaacttc tatcttcatt ttatttttga cctggttgac tttgtctgca tgcattttgt
     4741 atttaaccgc aacattcatg gttccgtttt ccactcctgt tacacgagct cccgacaagc
     4801 tggttaaaag cgatggcgca tctttcttta acccctctaa tacccgctgc caatcttgcg
     4861 cttcgacaac atgcggaaca gcaaccataa ctgggaccga gaccgtctct tgcatgggaa
     4921 caacaacact ttcttcctgc ggacgaggcg gaggtgccgt aatcgaaggt ccagccgcaa
     4981 tcgccggcgg agtagcaaca cggacctctg ccattggttg taccgcacgt gcagcctgcg
     5041 gcgcaacatt caacttggtt atagccacat caagcggtaa tcgaggaatc gggcttaact
     5101 tactatcgcc aatggcttct aagagtaatt cgagccactg cactaagcga gacaatcctg
     5161 ccccttgcac ggctgtcccc aacgcagacg gtaatgtctt aaattcctgc aaaacaaggg
     5221 cttcaaggca ctgctgactc aatgccagta attggtcaca aaaagctcgc agatctgcac
     5281 ctcgtgccaa tgcggcgtcc acacttccat gtgccttcac taagtcgccc gacagaattg
     5341 cggttaaaca ggttgccatt tgctcgacag ggatgtaacc aaacatttct tcaaccacct
     5401 gaagcgtata ttcaggatga ttggctgcgc gaacttgatc taaaagcgat aacgaatcac
     5461 ggaacgaccc gtcacctaaa cgggcgataa accgggctgc atcaggagcg aggttcatcc
     5521 cctcttgctc tgcaacgtac atgagatgag tagttaagcc atctaaagtt gccagccgaa
     5581 aatcataccg ctgacagcgc gaaataatcg tggcaggaat cttatgcgct tcggttgtgg
     5641 cgagaataaa gagggcgtgc gaaggtggtt cttcgagcgt tttgagaagc gcgttaaatg
     5701 cctctcgggt taacatgtga acttcgtcaa tgataaacac tttatagcgc gcctgcgtgg
     5761 gtgcgaacct gaccgaatct cgaagttgtc gaatctcgtc gatacctcgg ttagaagccg
     5821 catccacctc aataatatcg aggtttgcgc catccgtaat tgataggcag ttagagcaag
     5881 tattacacgg cttctcttct ccttcagcca aacagttcac aagtttggca atcaatcggg
     5941 cagtggtggt cttacccgtc ccgcgcggac cacaaaaaag atacgcctga ctcatctggt
     6001 tatgcgtcaa tgcataacgc aacgtatctc gaataacatc ctgacctttc aagtcctcga
     6061 atgtttgcgg ccgccatttt ctgtacaagg aaatttgtgt cgccattgca gctccgacta
     6121 taagcgatta gccctgcttt tactagcccc ttgacgaact acttagtacg cttgtgtgcc
     6181 tgccaaattt catagcctgt ggcaagcgta ttcaccgcca ataacagccc tccaaaaaag
     6241 aaatgcctcg agtaatcgtg tagattcaat gggacgaact gaccatttga catatcgctc
     6301 gggactggcg aaaaaataac cggcacaaga gaaaccgtcg gcgtgacgac aggcgcagaa
     6361 gttccctttg cggggaaaat tcgctgcacg agagaaatgg tacgacgcat aactttaggc
     6421 gcagtcgaga cgggcatgag agtagaagtc ggagcaacca ccggcaagac tgttggagct
     6481 ggcgtgctta agaggccagg cgacggattc aaggttggac tcgccgtcgg cactacgatt
     6541 gaagaagccg agggcgtagg aatagcacta accgagggtg aagggctcgg aaccgacgtt
     6601 ggcaggggag aaaaagtcac ggttggcgaa gggctcggcg ttgcggtggg taaaacggtt
     6661 ggggtcgtgc tcggagagtg acttggactg ggcgtcggag aaggcgcgac agagagcgtt
     6721 gggctcggag ttggcgtagt agttgccgaa ggggtaggtg tcggcgtgga aaactggcca
     6781 ataatcggga aaccgcgacc ggaaagcgca atcaaagcat agctcgtcat caggacgcta
     6841 ccaccccacg acccatctga gccttgagtc gacaaaagga aatcccgggg cgaatggcct
     6901 cccttactcc agggcgcata tgattgccca tattccgtaa gcatccaatc aacccaggcc
     6961 gtactcgcaa cactactgcc gcccgagcta atggcaaatc cgccatcgtc gttttgcaaa
     7021 gaacggatat aatccatcgc acgaactaga gattcttgag agaccgagcc ctttaaggca
     7081 atcaaagcga cggctgttaa atcagtactc gaagctcctc caacgattaa accccacccg
     7141 ccatcttggt tctgatgttg aagcaactga gaggaaacct ccgctggaac cgaagcgcct
     7201 gccgaccgca gggcaagaag accaaaaata tcgtcattca aataatctgt atcgccgatt
     7261 tgattcccca ttacccgcga ttgcaactca acaaccgcat ccacacccgc aaaagtacgc
     7321 gggttttcac ctcccgccac aagcgctaaa atttgctttt caatatctgt tgccgagcgg
     7381 gttgataatg aagtctgaac cgtctggaga gactgaatcg cgcgatcgtt acgctcgccg
     7441 taacccgaaa gcgccataac agcccaccca gtattaccga gcccactgat tgtcccatca
     7501 gggccttgct ttgatcgtaa aaaagctaag ccgtcatttg tgccagcaaa actagtgcca
     7561 atccaaccaa gtatgatgaa aaaactaaga attcgagcta ttttacgcat aattttgaaa
     7621 aaagtgggtg ggggcgctat gcgcccccga aaacaatcga tccaggaaga ggagtcgtga
     7681 gtctacgaag acttaccccg tcgggttcct ccatatcgat cacgtcttcc aattccacaa
     7741 ccaaccggca agagcgattt ggtaactcat accattctgc tccgacgttg atgaactcat
     7801 ctccgttgac cttgaacgtt atctgctgag agccgacgcc ccagacatac gtcctcgtta
     7861 ccgggtcgtg agaggccaca atccgccatc tccgataatt cactgccgta atgaagtcgt
     7921 ctggatattc cggatgctca tgagcacacg ttactccggc ctgacgaagg gattcaccca
     7981 acttcgcacc cggaacagct ttaaagctcc ctaccttctt gctcttaccc gcctgcgcga
     8041 agtaaaccat caccgaaatc ttcggtggcg gtcgacgggc tactcgggtt tgccgagaag
     8101 aacgcgccac ccctggagta tcgctccgcc gaaaagaaaa ctgcatttcc cccctctttt
     8161 ccgtagccac agtgctccgc ctttaatgcg cgaagcacag tcagacacag aattaattcg
     8221 ttttttcgta tcgccaggtt aatgtgtctc ccgccttaac caaaacttgg tctgcgctac
     8281 gattagctaa agcgccattg acgtaaaaga gccagtagta ccctgcccga ggattctgct
     8341 tcactccagc aatctcggtc acaaaccatc ccatatccga cacctcgtat ataaaactga
     8401 taccccgatt cgcggcaatc cgtttcgtaa gcagccccac aggctcctcc tgcgtttcta
     8461 tcttcccctc aaggtttact ccacctccca taattgaata accaacttct ctcagttgag
     8521 gagtaactgt gacgactgga tttggcgagg gagtaataat aactgttggc gcaatcgttt
     8581 cagcagttcg cacaacttga cttgtcgcca cctcgggcgt cactttcatt cgtggccgcc
     8641 tcgttggtgg agcaatcact gtctgttctc ttctcacgga gtttcccaca ccagcaggcg
     8701 tattaacgag cggggtcgac tcaaaagtac gttcaacgtg aggggaaacg tttgggacgg
     8761 attttacttc ggcaccctgg acatcacgga caagagtagt cccaaagaca catgtggcaa
     8821 gcattagtaa tccagagatc acacgcgata cgcctaaaga cataaaaaat cctcgccagg
     8881 ggccaggatc cataaaaatc aggatactga accacccatc ttccgtgagt tacgttatca
     8941 ggtgtctgtg ctggttttct gacttaccac cttcaggtgg attacagtag cggagactgt
     9001 cccggattta caccggattc cctaaagcaa ttccctcaac agaatcactt cttcacgcag
     9061 agacgacctt actatagcag gcaaagaagc attgtcaaac aagctctttc ctaactattt
     9121 tcctcggagt ggcaaaccat tgtagaaatc gctagttgct tttttagaag acttctatta
     9181 cggtgtgggt gtacaagaaa aagcggaggt gccactatgg accacgaaac agaaatgcca
     9241 aagaccgaca tgcccgtaga aggcgaagag atggagacca ccactcctga aggcgacacc
     9301 gaagaaggtg aagaagaaat gccagccaaa gatggcgaag gcgacgccga agatacagaa
     9361 gaaggcgaag agtaattcaa ctcttcatcg agttccaaaa agcgccccac cagggcgctc
     9421 tttggttata aaaacgaaag tggctgggtg tcgcatacag cgaccaccca gccaccagcc
     9481 caccccttat cgggcaggat tataaccagc aggagtccag atccagggtt gaccagcgac
     9541 aacccaaatc ttacccatca gctcaaattt cagaggtccc cactcccgga caatgctatc
     9601 cgagcaacga caacccgagg acggacaaat gcgatcacgt tcgtccatca cgcgaggtac
     9661 acttccttcc actccttcgt acgtcccagc ggccatccat ttggtttcac attccggatt
     9721 accgcaatcc agaaagatca taatcggccg ctcatctagc tcgctcattc ctccccctct
     9781 tcagaaacga tacgttgaaa cagtatacca tatatcaaga ataaggcctc ttaacacaaa
     9841 aaagcgcccg gggccaaaac cccgggcaaa cctaccttat ttccttcttt tgcaatcact
     9901 ccttcccttc gaactgaggt acgaatccga ggcgccgaga gacttccgcg acacctcgaa
     9961 tgtgcatctg gaagtaccca tcaaaccgag ttcctcctga gtaacatggc cccggcaaga
    10021 aaagtccaat tagatctctt tcgacggggg taagaccaga tggatccaga aggtaaacgt
    10081 aaggatcaac ccatcctcct tcgggaggtc gcttctgaag aaaagaagcc ggaaaccgac
    10141 ccgaaacggg gatacctctc cgtagctttt catcctcatc catctctccg gctaccttcc
    10201 aaaaatgcac tacccgtcca ctctcagagc taggcgtaag gatctctaaa ccgttaccat
    10261 accgcaggtc gagccactga aagggatttt cacgcttaac gcgagccgat tcttcgatcc
    10321 cctggacggt aaaccccagc cgatcagcta cagtacgaac aaccgtatga ggataccgct
    10381 gaaattgatc agtggctttt tcgaagatgt tgatttccac accatcaagt ccagcatgac
    10441 ttactcgacc gttctccgtc acaaaaagca ctttcatctg ctgttcccct ttcaccgcat
    10501 atagtttcaa ccaacattcg aagtcatcac ttgtgtgact accccgaaat tggtgccggg
    10561 gccgaagccc cggcaatctc tggccagcaa cgttcaacag aaccaaatgt gtttaacctt
    10621 acttagattc caggccgtac ttctcctcaa tttgtttcat agtctcgcct aactccttac
    10681 tccgttcgtg tgcatcctct cctgaccgca ggaggttacg ccgagcatcc ttggccaccc
    10741 aatacttctt aacgtcttcc acaacttccc gatgctgctc tccaacctgc tgcgcaacca
    10801 ttttccagac ctgctccaca acagatgttc cggtgttgta tataaagttg tctagcccat
    10861 gattgtcaaa cagattggtg agattcgtat ccacgtcaac ctcagtgcga atctcaatag
    10921 tgataactgt cttcatctca gttcctccac accaacaccc ggacacagga ccaacttcct
    10981 gttcccaaga ttggtgccgg ggccgaagcc ccggcgcaat tgttacttcc ctaactcccg
    11041 gtaaagaagt tataggactc cgcaccgctc ttaacgtgat agagagtgcc ataataccca
    11101 ccgcgacggc cggagagggt ctcagtgtca ccctgaagag tgatgtcatc gatctctacg
    11161 cgggtggact catctccgaa ggatgtgcag ccgctcaacc gcgtggcgtg tacccgtccc
    11221 cctgactgac gaggcccggt aagatctgcc agtcgcatcg gtggatttcc ccacagttcg
    11281 acttccatct caccctggag ctggtaatac gtcacattac ccttctcgtt gctcacctcg
    11341 agatacgcac ccttaccaaa acggactatc atctcgttcc tctttctcag cgggctggtc
    11401 cgcgttcgat atcatccagc tcgccagtca caagctgccg cgcttatgac tactgagcta
    11461 ggcccgacag ctggaggttc gtaaggaact tccttttgca atcgagcatt cgttgttaac
    11521 gttctcgctc gcttatcgtt acgagctgta atatacttca agacagtcgt tttgtaaata
    11581 taaatccacg atattttatt gacttttaac catatatatg aaaaactata cttagtttgt
    11641 cgaagcactt ccacacaaaa ataaggtaaa atatgcagcc aattatccac gaattaggcc
    11701 tctctcccaa agaagaagtc gtccttattg ccctcataga actgggcgca gcccaagtta
    11761 cccgcatcgc ccaaaaggca aatctcaacc gtacaacctg ttacgacatt ctcgagcacc
    11821 ttaatatcct tggccttgtg ggctatgtag ccgatcaaaa aaagaaaacc ttcgtggctg
    11881 agccacccga aacgctcgtt tcttacctgg aacggcggag taaacagttt gctgctaaag
    11941 ccgaagaagt ccgcaaagcg ctacccgaat taaaagcaaa gtacagcgaa cgtggcagag
    12001 ggcctaaaat acgcttctac gaggggaaag aaggccttaa aactgtctac gaggacacat
    12061 taactacgca cgaacctatt cgtgcctatg cttcagttcg caatatgcac gacgccctac
    12121 cagattattt tcctgagtac tataaacgtc gaaccaagaa aaatatctca atccgcgcta
    12181 ttcttcctgc tacacctgaa ggcattgaac gtagccagca tgacgccgaa gaagcacgag
    12241 aaagcgctct ggttgaccca gagcagttca atttctctcc cgaaattaac gtttatgaca
    12301 ataaaatcgc cattatgtca ttagccgagg aatttgccgt cattattgag tcaaaagaga
    12361 ttgccgaagc ccagaaaaag atcttcgagt tagcctggaa aggcgcaaaa tcagaagaaa
    12421 aataaaaaaa gcccgaggcg ccaacaaggc aacctcggac tcaaagaaca cctaacctta
    12481 ccctctatcc tctgaataac ttatggccca tccttccgca agagctaata acaactgacc
    12541 tcgacccatt caagaggctt gggcgagagc ttatcccgct ggccacgctc cactaccccc
    12601 aggccatcca gccccaagaa ctgaaggaca cgcccaatgc attcgcgctt gtcggcatcg
    12661 agctctttta atcgagccgc cttatggatc gctccggcct cccttaccac accaatcagg
    12721 cgagaggtgc cctcttcatc taactcataa acgctcaccc tccgcgtatc atcaagcaaa
    12781 tggtacgcac gattagcttc tgaccctctc cgcgtgcagt cgacgatact atcacgtata
    12841 tcctccggaa tgaggtccag cgatactttg tagtgcgacc agagccacac cttgtagccg
    12901 tccttatgac taccgtcgac gtgattccca tactcctcac acttcagatt ccgtaagatc
    12961 tgatcctcag aattttccaa ggacttgccg agcgcctcag cctctatacg gtacctgtag
    13021 tctgtgtaga agccattaaa gccaacactg atcgaaatgc cagtggtagc attaatagtt
    13081 acgttccagc caatgccatc tgccaccacg ttgggcgcga tttctcgggc agcgaacgta
    13141 taggcaaatc gacgaagctg aagcgccagc gcaatctgat tcatcttcct ctcctccggc
    13201 ttctggccgg acagcaacaa aaaggcacta cctccacctc ttcgcggcag acaataccat
    13261 tatctctgta ttatgtcaat agaactccac atctattaac ctatttacag gttagaatat
    13321 ccatgttatt atttatatgt cgacatttat ccacacatta tggaacagca actcactcaa
    13381 cttggccttt ctcctaaagc cgcccgaacc tatctggccc tcttggaatt gggtccttcg
    13441 agtattctgg ccatcgctag aaaagcaggc ctaaatagaa caaccttgta cgatattatt
    13501 ccagtcctaa tcacagaagg actcatttct cgtgttatta actccaaaac cgagaagtac
    13561 gaagcagaat ccccaactcg attaccactt ctactagagc gtaagattca ggaactacaa
    13621 ctccagatgg gtacggcaaa agatttagcc acaaaactag gatatcttgc tttcacgggc
    13681 aaagaatcaa agccaaaggt cactctgtac gaaggagaag aaggcataaa aaaaatgtac
    13741 gaagattccc tcctttgcaa aacatacatc cgctcttttc tggcccccga ggaattaaag
    13801 agttttgatg acgaatttgc cgatagttac tttgaacgcc gacgcaagaa aaacattcga
    13861 atccaaggga ttatcaatga ctctccggag tcgcggaatt atatcgagca cgaaaaagag
    13921 ctccttcggg atattcacct tgttccaact aatgcaatga atataaaacc cgaagtatac
    13981 atatacgaca acaaagtggc attttattca ctcaaagaga aatatggagt acttatcgag
    14041 tcagccgata tcgctgaggc aattgccaaa ctgtacgacc tcgcctgggc agagtcagaa
    14101 cgctctgaca aagagcttcg gatacaaaaa gctccgaaca gtaagtcgta atgccccacc
    14161 gccgtggaag acacttggca agtacacata attaaagtat acttttacta tcagccgcac
    14221 cttcctcacc gagaggtaca gagacgtgaa accaagaaac atgatcgtcc gcaatttgct
    14281 ttgcggaatg ggcgcgttcc tttgtctcaa tctgctctgc ctctccttgc gccccgaaag
    14341 cgtgtttgag aataagagcc tgagctactt tggaacatgc ctcgatacgc tcctgccgta
    14401 cgccctcagc ctgctcgtgt tcgcaggatt catacaccgc gcaatcgggc acatgccgcg
    14461 ggctaaacca tttgtccaga ccgcccgtat tctgcgaatc ttatcgctgt ttatggtcgg
    14521 cctcgtcgtg acgccttact cggttggccc agagatcaaa ctggcgcaca ccatcgttgc
    14581 catggtcgct tcggacatca tgctgggcct caccttttgg ctcgcactct tcgtccgtcg
    14641 cgaccgcgta agcgtctccc tgcttataac ccaggtaact gcagggatac tggccggact
    14701 cgccctcctc gataaaggcg gcctatttct cgccggagag atggtctacg tccttgcctt
    14761 tggggcaatt ctcgcccgca ccattcacct cacccacccc gccccgtaga caaaactacg
    14821 gggcggtttg catactaaat ccaaagttac ggattcaacg agttccacac ctgttgaaag
    14881 ttaaaacgtt cgagctccgt taactgtttt gattcaagaa caatcagatt tgttgcattc
    14941 tcgctgatcg ttaaatatga aacagtgtta ttccaaatcc acttctcggt agccgtatgc
    15001 caccatgtac gcgtaaatcg gtattgtgcg tgtgactgcc ctaatttagt cgaaaataaa
    15061 cgcgattcgg gcgtatccgg agcaataatt cgatctttta tacccattcg ctgcttaaat
    15121 gccacaaatt gctctaaaaa atcagggtcg acacttaacc aggtcttatt ggttgcccaa
    15181 atccatagct catctccttc tgcagcctcc tccataaacc gacgatacca ctcccgcatg
    15241 ctaatcgggt tttcgtatac cgagagtatt ggcagggcgt tattagtcgc ctgcttcgtc
    15301 tttaaaaccg gtacggcttt ttttacttca gtcaaaatat ctgccgcacg atcttgcagt
    15361 acctctgggc tcaacgcttc aaacaaccgg cgactcccca ctaccgtttc cttaatgagg
    15421 ccttgctcta agagcgaagg cagcaacgta taaaccatct gacgcgagat tcccactttc
    15481 tgcgatagct gaatcgccgt ggccggcccc aaacgtaaat ttgccaagta taatttggcc
    15541 tctgtctcac taaacccgat aaacgttaac aacttcgcat aatccataga cacctatttt
    15601 gtaatatttt attttacatt atcaaacctt atacactaat ctttgcatat acaaaccagt
    15661 atagtcaagt tatatagttg tcataaagta gttgactaat aatatctact ccgttatatt
    15721 tacgccgtct caaccagaga caaagcagct tcacaagtac atcctagacc ggcaacaacc
    15781 atgaaagatc cacacgctcc gcccaccagt ctcaccatta tgcaacctcc ccgggaaccg
    15841 ttctttatct tctccgctga cggcatcttt tacagcctta acggcgaagg ggcaacgatc
    15901 ggctaccccg cggtgtacgc gcgactccac ggttgcaatc ttaattgcag ctggtgcgac
    15961 actccctaca catggaaccg ttccctaaca gagttccatg agtacctcca gagaggaagg
    16021 ctatctatcc ctgacacact cgagcaactc atcgctgcag acgtaagtgg ctgcaaacgc
    16081 ctggtcttca ctggcggaga gcctctgctc cagcagaagg caatcgcagc catactgccc
    16141 cagcttacgc actggtcggt cgaagtcgag actaatggga ctatcgcgcc tcaaaaggag
    16201 ctctttgact gccagttcaa ctgttcaccc aagctaccta actcaggtat ttctgccgag
    16261 cgagccgtaa agcctcaagc cattcacacc ttaaaagggc ttaataccta cttcaagttc
    16321 gtggttcgga ccgccaacga cgtggccgag atgcaacgta tctacgagca accgttccag
    16381 attccacgcg acaggatcat cctgtcaccc gaagggatca ctgtcagcga cacggttcgc
    16441 gtcatgcaaa atctagcaga gattgccaaa gatcaaggct accgcctcac tccgaggctc
    16501 cacatcctac tctggggaga cacccgaagg acctagccaa tcatgtcact tcaagtcagc
    16561 gttacgaaga tcttcacgtt cgagtcagca cactctctcc cgagccatcg ggggaagtgt
    16621 cgtcggcctc atggccactc gtacaagctc gaagttactc tctcggggcc actaaaggcg
    16681 cgggggagct cacaaggtat ggttatggac tttgccgacg tctctaaagt tgtaaaacga
    16741 gagattatca caccatggga ccaccagtcc ctgaacgacc ttttctcgtt tccaacaacg
    16801 gccgagaacc ttgctctgga ggcatttcga cggttacgcg ctgcaaaact gaccgtaact
    16861 cgagttcgcc tctgggaaac ggccacttct tatgccgagg tagaaagtca tgacatggga
    16921 taacagaatc gtcgccgcct atcaagcaag cgacgagaaa ccagacaaaa agtactcgat
    16981 cggcccgaca gtagtcggcc tggccgatcc agagggtaaa gaagtcctgg acctagggtg
    17041 tggcagcggc tttttcacta gaagactcat ctgggcaggc gccaagcgag tcatcggact
    17101 cgataactgc cccgaaaaga tccgaaaagc caaaatccag ccccttcccc gcctagagta
    17161 ccgacttggg gacatgttca cggatgaatt gccgtccgta gacgtcgtaa ccgccccatt
    17221 cacgctcaat tacgccagaa acgaagatca gctccttctt ttgcttgcga ggctctacgc
    17281 gagcctcagg gcgggaggaa gactcgtgag tgcaatcgac ctacccgaag ggagagacct
    17341 tatccggttt ggagcccgga aaacgctcct tggacccaaa caagacggca ccctgatcca
    17401 gatagaactt tttaaccaag gtctacactt ctgcacactc ccgttagcgg cccactactt
    17461 cgctccagcc accatcgagc gaactcttac gcagattggg ttcatcaacc taacctggca
    17521 caaaccaatt atctctccag aaggctttaa agcgttcaaa aacgccccag acttctggaa
    17581 agactaccca gaccaccccg aactcggtta tataaccgcc caaaagccgc ccagcaaaag
    17641 ctaggcggct tttattataa aaagtgccga ccacgccgat tcctcggcat ggcgggcaaa
    17701 cgtaaaggcc attactactt gggagtgtaa tcattgtcga tttcacctcc ccactggcct
    17761 gacgaggcaa caaagctccc tcggagtgca tggcgaaggc ctcgaggaac aacaatcagg
    17821 ttcccttcgc caattgcgta ctcctccttc cccagcacac agactcccgc acccgatatc
    17881 acgtagataa aagagtcgga attctggtgc acatgcgctc gcgcttcaga gatgtacact
    17941 cgggaatatt ggggtactct accaatggca aagtactcat acccaacccc gcggggtaaa
    18001 tccgtctctt tccacgcgga ccaaagcgcg atatccatgg ggcgttgcgt taacgggatg
    18061 gcgtcgatcg cactccatac acgagtccta acagactgat cctgttcagc attacgctga
    18121 atgacaatta tccgaactgc at
//
LOCUS       DASWYB010000088         5949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_46157, whole genome shotgun sequence.
ACCESSION   DASWYB010000088 DASWYB010000000
VERSION     DASWYB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46157"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VGE59_04975"
     CDS             <1..420
                     /locus_tag="VGE59_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY1075011.1"
                     /translation="ETLHFASILLSAGARLKDITAHISQLTSVAKLKLWGVALSRIHQ
                     HPELGIISTYITIADILQTGASEEDIAGIATLLSSIPSEMAVIALEMPSGEVQVRMRT
                     KSRAVNLSRLTSYLGGGGQKKSAGFSFKGSLNEFITK"
     gene            complement(479..563)
                     /locus_tag="VGE59_04980"
     tRNA            complement(479..563)
                     /locus_tag="VGE59_04980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(528..530),aa:Leu,seq:taa)
     gene            650..1306
                     /locus_tag="VGE59_04985"
     CDS             650..1306
                     /locus_tag="VGE59_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1075012.1"
                     /translation="MRKKLSILISVLIVIALGVGIYFAVPNIFGSVIVPLPDEYKGLV
                     EKYAKLNGLDPCLVAAVINGESRWNAGASSHVGAQGLMQVMPATKRAVEKKFGLPSGP
                     ATDPESNIRVGTRLLRYNFDSYGSLRNVLVAYNAGGSRVVRPDNQLPKETQFYIVKIT
                     RYYELYKAVYPTFCAATTSANPKAAPEPDLPDFVVPAPIESPDLLDPNNFWKAFLPES
                     "
     gene            1319..3058
                     /locus_tag="VGE59_04990"
     CDS             1319..3058
                     /locus_tag="VGE59_04990"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY1075013.1"
                     /translation="MTHQDIRKKFIDYLISHGHVELPAASLVPDNDPTVLFTVAGMQQ
                     FKQLYEHPDQAPSKRVVTIQPCIRTIDIDEVGDDTHSTVFEMMGWFSFGYTGSEDTLH
                     GEDPYFKNTAIKMGWEFLTKELGVDPNRIRATYFGGDAKRPADVESKEILESLEGLSL
                     IEATGENFWGPVGEEGPCGPNAEFYIDDTEVLNNVFNQYIRDTEGNYTQAEYRGVDMG
                     TGFERLVAAIQGVPNIYETDLVKPFLQCVRAKSYNYNERHGRIIVDHVKAALYLLADG
                     VKPGNKGRDYVLRRLIRRSVRAAQIVGFQEFEALLNMLADEYGTTRDAIAKNRDGAIA
                     LFLSERDKFLKTLQDGTSQLKKLIGNERGGEITGEQAFKLFDTYGFPIELTQEMAEEL
                     GWTVDADGFTDELNKHRELSKQGSEKIFKGGLADHDPQTIAHHTAHHLLLAALREVLG
                     DHVVQRGSNVTSERLRIDVAHPEKITDDQIKTAEDIVNQKISEDLPVISEIMDRDEAF
                     KLGALAEFGAKYPDKACVYSIIDQDGEVFSREFCGGPHVMFTGELGHFEIIKEEASGA
                     GIRRIRARVVPRT"
     gene            complement(3105..3536)
                     /gene="dut"
                     /locus_tag="VGE59_04995"
     CDS             complement(3105..3536)
                     /gene="dut"
                     /locus_tag="VGE59_04995"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY1075014.1"
                     /translation="MTIRLKRFDPSVSIPEYKTPGAAAMDVSARIDVTILPKQVGKIP
                     LNFALDLPDGYFSLIAPRSSTHKLGLMSGNSIGIIDSDYRGDNDEYIFLAYNFTDNPV
                     TIEKGTRVAQMLILPVSRVRFEEVATLDTPDRGGFGTTGHH"
     gene            3601..4854
                     /locus_tag="VGE59_05000"
     CDS             3601..4854
                     /locus_tag="VGE59_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEY1075015.1"
                     /translation="MGIFNLGARRSRRTQSAIALDIGTEYVKALIFTVDSDGIGHIVG
                     VGRQHQKLSDMQGGTITDIAGVVHNCELALEQAAEQAGFLPDNVIMGIAGELVKGTTT
                     TITITRPQPTQKITMPELQKLIADVQKRAFDQARDVIGFESGVEQVDVRLVNAAIVDV
                     TIDGYKVANPIGFQGKEVKVGVFNAFAPLVHLGAYQTVADELKLDLLSIAAEPYAVSR
                     LYGPEMTQDFSAIFVDIGGGTTDIAVVQNGGVLGTKMFAIGGRAFTKRIATVAGVPFS
                     EAEEMKVNYSAKKTNGEDTELIKHALVSDANVWVSGVELTLSEFMNIDFLPSRILLCG
                     GGSNLPEIKELLKTDWHTDLPFAKKPTVHFIQPEEVTTLVDETGKLKAPQDITPMALA
                     NLALELVDTEGPIDSILNRVIAATK"
     gene            4865..>5949
                     /locus_tag="VGE59_05005"
     CDS             4865..>5949
                     /locus_tag="VGE59_05005"
                     /inference="COORDINATES: protein motif:HMM:NF044707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075016.1"
                     /translation="MNSFEEIYLETDEEVTSAIDKIKKSKKKNVALCLPRHAVMGQSI
                     VNLKLLYKEAIASGKKLALVSSDKITRALAEKVGFFTFEQASDIVFDKDEIEEAPEPK
                     KQLKEAEEVSITRERFPVSEKEPQEEVSEQPAPATPTVKGFTKQTVTDIPEEELKESE
                     QSPLDDKTESSPEVKSSRPKSSMIPTKGNLRFYRQSKRPAFLIPLLIAVAVVVIGGVS
                     AVLAIPRATVDITVSAQPFNETVNSNVDIETTAVDVTKSTIPGKLVSVEYETKSSAKA
                     TGKKDTGEKATGTVLLINEWDSLNHTFEAGTKLRAKNGNEFVLTEDVVLPGASSTLSG
                     GKTVITSGKKEANVVAAAAGPNYNNAAT"
BASE COUNT         1675 a         1215 c         1496 g         1563 t
ORIGIN      
        1 gagacgctgc actttgcttc aattcttctc agtgcgggcg ctcggttaaa agatattacg
       61 gcccatattt cgcagctcac ttcggtggca aaacttaagt tatggggagt tgcgctcagt
      121 cgcatccatc agcatcccga actgggaatt atttctacct atattacgat tgccgatatt
      181 ttgcagacag gcgcctcgga ggaagatatt gcaggtatcg cgaccttgct ttcgagtatt
      241 ccgtccgaaa tggccgtcat tgctctcgag atgccgagtg gggaggttca agtcagaatg
      301 cgcacaaaga gtcgtgcggt taacctttcc cgccttacga gctacttagg aggcggtggc
      361 caaaagaagt cggccggatt ttcgtttaag ggtagcttaa acgagtttat aacgaagtaa
      421 gtgggcacaa tattattgta ccaacctaaa aacgagcaga tcctgctcgt ttttaggttg
      481 gtaccgaggg cgggattcga acccgcaagc cttttgggca aaggatttta agtccttcgt
      541 gtataccgtt ccaccacctc ggctaacagg tttattataa cataagctat aaataaaata
      601 ctacagctgc ggactattga gaaaaagctt ttttaggtat actaaatagg tgaggaaaaa
      661 actttctatt ctcatcagtg tactcattgt tattgcgctg ggcgtaggga tttattttgc
      721 cgtaccgaat atttttggaa gtgttattgt tcctctcccg gatgaatata aaggtctcgt
      781 ggaaaagtat gcaaaattaa acggactcga cccttgtctt gtggcggcgg taatcaacgg
      841 agagagtcgt tggaatgcag gtgccagttc tcacgtggga gcgcaaggct taatgcaggt
      901 aatgccagca acaaaacgag ctgttgaaaa gaagtttggt cttccttccg gtcctgcgac
      961 tgacccagag tcaaatatcc gagtcggcac acggttacta cgctataact ttgattcgta
     1021 tggctcattg cgcaacgttc tcgtagctta caatgcgggc ggaagtagag ttgttcgtcc
     1081 ggataatcag ctccccaaag aaacgcaatt ctatattgta aaaatcaccc gatactacga
     1141 actttataag gccgtttatc ctaccttctg tgccgccaca acctctgcga accctaaggc
     1201 cgcaccagaa cccgatttac cggatttcgt ggtgcccgca ccgattgaga gtcctgacct
     1261 cttagaccct aataattttt ggaaagcatt tctccccgaa tcataattaa ggatatgtat
     1321 gacgcatcaa gatattcgta aaaaatttat tgattactta atctctcatg gccatgtcga
     1381 gttgccggca gcttccttag tacccgacaa cgacccaacg gttttattta cagttgcagg
     1441 aatgcaacaa tttaaacagc tttacgagca tccagaccag gcgccgagca agcgtgttgt
     1501 aacaattcag ccatgtatcc gtacaattga tattgatgaa gttggtgatg atacacacag
     1561 tactgtattt gaaatgatgg ggtggtttag tttcggctat acaggaagtg aggatactct
     1621 tcacggtgaa gatccttact ttaaaaacac tgccataaaa atgggatggg agtttcttac
     1681 gaaagaactc ggcgttgatc cgaatcgaat tcgagccact tactttggtg gtgatgccaa
     1741 acgccctgca gatgtagaat caaaggaaat tttagaatct cttgagggat taagcttgat
     1801 tgaagcaacg ggagaaaatt tctggggtcc agtaggcgaa gagggaccct gtggacctaa
     1861 tgccgaattt tatatcgatg ataccgaagt tctcaataat gtctttaacc aatatatccg
     1921 agatacagaa ggcaattata cgcaagctga gtatcgaggg gttgatatgg ggactggctt
     1981 tgaacgttta gtcgccgcta tccaaggtgt acccaatatt tatgaaacag acttggtgaa
     2041 accattctta cagtgtgttc gtgcaaaaag ctacaattac aatgaacgac atggccgtat
     2101 tattgttgac cacgtgaagg cggcacttta tttattggcg gatggggtaa agccgggtaa
     2161 taaaggccgt gattacgtgt tacgtcgctt aatccgtcga tcggttcgtg cggcgcagat
     2221 tgtcggattc caagaattcg aggcgctcct taatatgctc gcggacgaat atggcacgac
     2281 acgtgatgcg atcgccaaaa atagagacgg agccatcgcc ctctttctct ctgagcggga
     2341 taaattttta aagacgcttc aagatggtac gtcccaactc aaaaagttaa ttgggaatga
     2401 acggggaggc gaaattacgg gagagcaagc ctttaaactc tttgatacct acgggttccc
     2461 gattgaacta acgcaagaga tggctgaaga gttgggttgg accgtggatg ccgatgggtt
     2521 tactgacgag ttaaataaac atcgtgaact ttccaaacaa ggttcggaga aaatctttaa
     2581 aggcggtttg gctgatcacg atccacagac tattgcccat catacggcgc accatttact
     2641 cttagcagcg ttgcgcgaag ttttgggtga tcatgtagtt caacgtggtt caaatgttac
     2701 aagcgagcgg cttcgtattg atgttgccca cccagaaaaa atcactgacg accagataaa
     2761 gacagctgaa gatatagtta accaaaagat ttcggaagat ttaccggtta tcagcgaaat
     2821 tatggatcga gatgaagcat ttaaactagg ggctcttgca gaatttggtg ccaaatatcc
     2881 tgataaagct tgtgtgtaca gtattattga tcaggatgga gaggttttct cgcgtgaatt
     2941 ctgtggtggt ccgcatgtca tgttcaccgg tgaacttggc cactttgaaa ttataaaaga
     3001 agaagcaagc ggggcaggga tccggcgtat tcgcgctcgg gtagtccctc ggacataata
     3061 tatcccctta taacaaagaa ctgcctccaa gaggcggttc tttgttagtg gtggcctgtg
     3121 gtgccgaatc ctccacggtc cggggtgtcg agtgtcgcaa cttcctcgaa gcgtactcgg
     3181 gaaacgggta gtatgagcat ctgagccacg cgggtacctt tttcaattgt cacagggtta
     3241 tcggtaaagt tgtaagcaag aaaaatatat tcatcattat ctccccgata gtccgaatca
     3301 atgataccga ttgagttacc cgacattaac cctagcttat gcgtagagct acgaggggca
     3361 attaatgaaa agtagccatc aggtaggtcc aaggcaaaat ttaaaggtat tttaccgact
     3421 tgttttggaa gaatcgttac atcaattcgg gcgctgacat ccattgcagc ggcgcctgga
     3481 gtcttgtatt caggtatgga tacggagggg tcgaagcgtt tcaagcgaat agtcatggaa
     3541 acattgtagc atacgtcacg acctgggttt tctttctcat ttatggcata gtaaaaaggt
     3601 atgggaattt ttaatttagg tgcacgtcgt tcccgtcgga cgcaatcagc gattgcttta
     3661 gatattggga cagaatacgt aaaagccctc atttttacag ttgattcaga tgggataggg
     3721 catattgttg gtgttggtcg gcagcatcaa aaactttcag atatgcaagg cggcaccatt
     3781 actgatattg cgggtgttgt tcataactgt gaactcgctc tagagcaggc ggcagagcaa
     3841 gccggattct taccggataa tgtgattatg gggattgccg gagaacttgt aaaaggaacg
     3901 accaccacga ttacaattac acggccgcaa ccaactcaaa agattaccat gccggaatta
     3961 caaaagttaa ttgccgatgt acaaaaacgg gcttttgacc aagctcgaga tgttattggc
     4021 tttgaatcgg gggtggagca agtagacgtc cggcttgtga atgctgccat cgttgacgtg
     4081 acgattgatg gctacaaagt ggctaacccg atcgggtttc aaggcaaaga agttaaagtt
     4141 ggggtattta atgctttcgc tcccttggtt catttgggag cctaccaaac ggtagcggat
     4201 gaactcaagc tcgatttact ttccattgct gctgaaccct acgcggtctc ccgtctctat
     4261 ggtcctgaaa tgacccaaga cttttcggcg atctttgtgg atattggggg ggggacaact
     4321 gatattgctg tcgtgcaaaa cggtggcgta ttgggtacga aaatgtttgc tatcggaggc
     4381 cgcgctttta ctaagcgcat cgcaacggtg gcgggggttc ccttctctga agcagaagaa
     4441 atgaaagtta actacagtgc caaaaagaca aatggggaag acactgaact tatcaaacat
     4501 gctctggtga gtgatgcgaa tgtttgggtt tctggggtcg agctaaccct gtctgaattt
     4561 atgaatattg attttcttcc gtctcgaatt ctgttgtgtg gcggagggag caatcttccc
     4621 gagatcaaag agttattaaa gactgactgg catacggatt tacccttcgc aaaaaaaccg
     4681 acggtccatt ttatccagcc tgaggaagta acaacgctgg ttgatgagac gggaaaactc
     4741 aaggctcctc aggatattac accgatggcg ttggcgaact tagcgttaga attggttgac
     4801 acggaggggc caattgatag tattttaaat agagttattg ccgcaacaaa gtaaaggcct
     4861 cgaaatgaat tcatttgaag aaatctattt agagaccgac gaagaagtga cgtcggcaat
     4921 cgacaagata aaaaagagca aaaagaaaaa tgttgctctc tgtttgccgc gtcatgcagt
     4981 tatgggacag agtattgtca atttaaaact cctctataaa gaggcaattg ccagtggcaa
     5041 gaagctggcc ctggttagct ctgataaaat cactcgggcg ttagccgaga aagttgggtt
     5101 ttttacgttt gaacaagcga gcgatattgt ctttgacaaa gacgagattg aggaagctcc
     5161 ggaacctaag aagcagctaa aagaggctga ggaagtttcg attactcgag aacggtttcc
     5221 tgtttcagaa aaagagccgc aagaagaggt gtctgagcaa ccagctccgg cgacaccgac
     5281 ggttaaaggc tttaccaaac aaactgttac ggatatccct gaagaagaat taaaagaatc
     5341 agaacaatct cctcttgacg ataaaacgga gtcgagccca gaagtaaagt catcacgtcc
     5401 caaatcgagc atgattccta ccaaaggaaa cttgcggttc tacaggcagt caaaacgtcc
     5461 ggcattctta attcctttac ttatcgccgt tgctgttgtc gtgattggag gtgtctcagc
     5521 agttctcgca attcctcgtg ccaccgttga tatcaccgtg agtgcccaac catttaatga
     5581 aacagttaac tcaaacgtgg atattgaaac aacggctgtg gatgttacga aatcgactat
     5641 tccaggtaag ttggtttcgg ttgaatatga aacgaagtcg tcagcgaaag caacaggaaa
     5701 aaaagatacg ggggagaagg caacgggtac ggttcttttg attaatgagt gggatagttt
     5761 aaatcataca tttgaagcag ggacgaaact acgggctaaa aatgggaatg aatttgtcct
     5821 gacggaagat gtcgttctgc ctggtgcctc ctcgacgttg tcgggtggga agacggtcat
     5881 tacatcgggt aagaaagaag ctaatgtggt ggcggctgcg gcaggaccaa attacaacaa
     5941 tgccgctac
//
LOCUS       DASWYB010000089         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_153163, whole genome shotgun sequence.
ACCESSION   DASWYB010000089 DASWYB010000000
VERSION     DASWYB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153163"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1049
                     /gene="ychF"
                     /locus_tag="VGE59_05010"
     CDS             3..1049
                     /gene="ychF"
                     /locus_tag="VGE59_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY1075017.1"
                     /translation="MFNALVKSSVAEAANYPFCTIEPNVGIVDIPDEKLPQLAQIAKA
                     EKIVPAAIEFVDIAGLVRGASQGEGLGNKFLSHIREVDAIILVLRGFHNENVINTQDS
                     VHPERDAQIIDLELILADMETVSKRKESIHKDLKAGKEEAQILAKALELALPHLESEQ
                     PLRTLSLTDKQANALQQMQLLTMKPFMIVLNADENEIGNPIEALRERYVPNLSQNYPL
                     VPVSAKIESEIAQLSQEEAKEFMESLGMTESGLDKVAKAAYSLLGLQSYYTAGEKEAR
                     AWTIKQGSTAPQAAGVIHGDFERGFIAAEIISFNDFISLGGWSGGRPAGKVRTEGKTY
                     VMQPDDVVLFRFNV"
     gene            complement(1076..1306)
                     /locus_tag="VGE59_05015"
     CDS             complement(1076..1306)
                     /locus_tag="VGE59_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075018.1"
                     /translation="MVFKYGAIGLVLGVSTVFLSVIIVDALLGGVVDTTLAAASSLNL
                     WSALGIIVLYTLLAIGVGEILDSAHRDTKSKK"
     gene            1482..2027
                     /gene="rpsF"
                     /locus_tag="VGE59_05020"
     CDS             1482..2027
                     /gene="rpsF"
                     /locus_tag="VGE59_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY1075019.1"
                     /translation="MKTYEVTYLTLGEEGYDASTISETLAKHGCKIVSVHPWGARRKL
                     AYPINKQDQAFYTTVVFDADASAVLPIENALKLNNDVLRALVTTFEPGYFQRMANTTA
                     PTSGSEAKESEKPKAEEVKEETPSEEVAPIEEVTEEAITEETPAAEEKPKRRTTKNAD
                     KEDSQALDEKIDALLNEDITK"
     gene            2094..2558
                     /locus_tag="VGE59_05025"
     CDS             2094..2558
                     /locus_tag="VGE59_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY1075020.1"
                     /translation="MFSLNRATLIGNLTRDPELRQTPSGQSVASFAVATNRRWTTQQG
                     DTQDATEFHEVVAWGKLAEISEQLLGKGKKVYIEGRLQTRTWDAPDGSKRQRTEIIAE
                     NIIALSPKGGEGMGGGDSFPSTQSKTTKAKSASQDAPAATGGDEIDLSDLPF"
     gene            2649..2858
                     /gene="rpsR"
                     /locus_tag="VGE59_05030"
     CDS             2649..2858
                     /gene="rpsR"
                     /locus_tag="VGE59_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY1075021.1"
                     /translation="MAKQRKQCFFTKKNIRPDFKDVSLMQRYITPWGKIKEGRDTGTC
                     AKCQRQLSTAIKRARFLAILPYSTR"
     gene            2863..3429
                     /gene="efp"
                     /locus_tag="VGE59_05035"
     CDS             2863..3429
                     /gene="efp"
                     /locus_tag="VGE59_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY1075022.1"
                     /translation="MATLAIGDIRPGVAIIWNGQPHIVIWREHAMMGRGGGFVRTRLK
                     NLINGSTVENTFKGNDKIDEADLFRSQAQFLYFEGTDPVFMDVATFEQFTLPRDQIGE
                     SAGFLTEGMNVDVLSYQDRPITVTLPIKVELKVTYTEPGFKGNTASGTNKPATLETGA
                     HIQVPLFINQGDTIRVDTRDGSYVERVK"
BASE COUNT         1091 a          890 c          783 g          764 t
ORIGIN      
        1 ctttgtttaa tgccttagtt aaatcatctg tagcagaagc tgcgaactac cctttttgca
       61 cgattgagcc aaacgtgggc attgttgata ttcccgacga gaagcttccc cagttagccc
      121 aaattgctaa ggcagaaaaa atcgtaccag ccgccattga atttgtagat atcgctggcc
      181 tagtccgtgg agccagccag ggcgaaggat tgggtaataa atttttaagt cacatccgtg
      241 aagtagatgc tattattctc gttttacgtg gctttcataa cgaaaacgtt attaataccc
      301 aagatagcgt gcaccctgaa cgagatgccc aaattattga cctcgaatta attcttgccg
      361 acatggaaac agtatccaag cgaaaagagt caatccacaa agacttaaaa gcaggtaaag
      421 aagaggcaca aatattggcc aaggcgctag aactagcctt acctcacctg gaaagcgaac
      481 agcctcttcg aaccctctca ctcaccgata agcaagctaa cgccttacag caaatgcaac
      541 ttttaaccat gaaacctttc atgattgttt taaatgccga cgaaaatgaa attggaaacc
      601 ctatagaagc attacgtgaa cgctatgtac cgaacctgtc tcaaaactac ccactcgtcc
      661 cagtcagcgc caaaatagaa tccgaaatcg ctcaattatc ccaagaggaa gcaaaagaat
      721 tcatggaatc cctcgggatg accgaaagcg gattagataa agtcgccaaa gcagcctact
      781 ctctcttggg cctccagtcg tactataccg ccggcgagaa agaagcccgg gcgtggacta
      841 ttaaacaagg aagcaccgct cctcaagcgg ccggtgttat tcatggtgat tttgagcgag
      901 gatttattgc cgcagaaatc atctcattta atgattttat ttctcttgga ggatggagcg
      961 gaggacgacc tgccggcaaa gtccgcaccg aagggaaaac ctacgtcatg caaccagatg
     1021 acgtagtcct ctttcgtttt aacgtataag aaaaacggcc ttgcggccgc tcttcttatt
     1081 ttttagattt tgtatcgcga tgggctgaat ctagaatttc gccaaccccg attgctaata
     1141 aagtgtaaag tacaataata ccaagtgcgc tccacaaatt aagagaagaa gcagcggcaa
     1201 gcgtagtgtc tacaacacca cccaaaaggg catcaacaat aataaccgat aaaaataccg
     1261 tcgagacacc taaaacaaga ccaattgcac catatttaaa taccatagtg aactccttta
     1321 ctatctagcc tgcctgaaat aaacacaaaa agcaatctaa tccattgtgc ttaacagaga
     1381 aaaatggtat agtacgaggg tcattggtaa cgattgtcct gcttgtgtaa gcaggtccaa
     1441 tacttcccaa caatcgtaat ccataaacaa gaaggaagag agtgaaaaca tacgaagtta
     1501 cctacctcac cctcggtgaa gaagggtacg atgcatcaac cattagcgag accctcgcaa
     1561 agcatggttg taaaatcgtc tctgtacacc catggggcgc ccgtcgtaaa ctggcttatc
     1621 ctatcaacaa acaggatcaa gccttttaca ccactgttgt ttttgacgca gatgcatcag
     1681 ctgtactgcc aattgaaaac gccctcaaac tcaataacga cgtgcttcgc gcactcgtga
     1741 cgacgtttga acctggttac ttccaacgca tggctaacac caccgcccca acaagcggca
     1801 gcgaagccaa agagagcgaa aagcccaaag ccgaagaagt aaaagaagaa actcctagtg
     1861 aagaagtcgc tcccatcgaa gaggtgaccg aagaagctat cacggaagag acacctgcgg
     1921 cagaagagaa accaaaacgc cgcacaacca agaatgccga caaagaagat tctcaggctc
     1981 ttgacgaaaa gattgacgca ctgttaaacg aagatatcac caagtaacat taagtaagtg
     2041 gagcgagtca ggacgacttc tggtcgcctg ctcctcgaag taaaggagta cctatgttta
     2101 gtttgaaccg cgctaccctc attggaaacc tcacacgaga tccagaactt cgccagacgc
     2161 caagcggcca atctgttgca agtttcgccg ttgcgaccaa ccgtcgttgg acaacacaac
     2221 aaggggacac tcaggacgcg actgaatttc acgaagtcgt agcctggggc aaattagcgg
     2281 aaatttctga acaactcctt ggaaaaggca agaaagttta catcgaaggg cgcttacaga
     2341 cccgcacttg ggatgccccc gacggatcaa agcgccaacg aaccgaaatt attgccgaaa
     2401 acattattgc cctcagcccc aaaggcggcg aaggcatggg tggtggcgat agtttcccct
     2461 caacccaatc aaaaacaaca aaagcaaaga gcgcatcgca agatgctccg gctgcaacag
     2521 gcggagacga gatcgactta tccgatcttc cgttctaggc tcgacagctg aaggattgtt
     2581 cgagccaccc atgaagacag gatagcgaaa aatagtccgc agcccacaac atacttaaaa
     2641 ggatacacat ggcaaagcaa cgcaagcaat gtttcttcac caagaagaac atccgccccg
     2701 actttaagga cgtatcgctc atgcagcgct acattacccc ttggggcaaa attaaagaag
     2761 gtcgagacac tggcacctgt gccaaatgtc agcgtcagct ctcaacggcg atcaaacgcg
     2821 cccgcttctt agcgattctt ccttactcaa cccgttaatc atatggcaac actggcgatt
     2881 ggcgatattc ggccaggcgt ggccatcatc tggaatggac aaccccacat tgttatctgg
     2941 cgcgaacacg ccatgatggg acgtggtggt gggtttgtcc gtactcgcct caaaaacttg
     3001 ataaatggat caaccgtaga aaacaccttt aaaggcaatg acaagattga cgaagccgac
     3061 ttgttccgtt ctcaggctca attcttgtac ttcgaaggta ccgacccagt attcatggac
     3121 gttgcgacat tcgaacagtt cacacttccc cgcgatcaaa taggggagag cgcaggattc
     3181 ctcacggaag gcatgaatgt tgacgtactt tcctatcagg accgtcccat cacggttaca
     3241 ttaccgatca aagtagaact caaagtaacg tataccgaac ctgggtttaa agggaataca
     3301 gcatctggga caaataagcc tgccaccctt gagacaggcg ctcatataca ggttccattg
     3361 tttatcaatc aaggtgacac aattcgcgtc gacacccgag atggctcgta cgttgaacgc
     3421 gtaaagtaat aaggagccgg ggcatgacgt acacatcagc ccgttcttac cttggattgc
     3481 ccctcattag cgctgactcg gcgcgaagtg gcggggcaat ttttggta
//
LOCUS       DASWYB010000090         9236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_509286, whole genome shotgun sequence.
ACCESSION   DASWYB010000090 DASWYB010000000
VERSION     DASWYB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509286"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..232
                     /gene="infA"
                     /locus_tag="VGE59_05040"
     CDS             14..232
                     /gene="infA"
                     /locus_tag="VGE59_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY1075023.1"
                     /translation="MEEKEFVEVQGTVTEALPNRMFRVKLDNEAMVLGILSGQMARHK
                     IRVLLGDRVTLELPIIDLTKGRIIRRLS"
     gene            250..366
                     /gene="rpmJ"
                     /locus_tag="VGE59_05045"
     CDS             250..366
                     /gene="rpmJ"
                     /locus_tag="VGE59_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY1075024.1"
                     /translation="MKVRPSVKKMCDTCQIIRRKGVVVVLCKKSPRHKQRQG"
     gene            379..765
                     /gene="rpsM"
                     /locus_tag="VGE59_05050"
     CDS             379..765
                     /gene="rpsM"
                     /locus_tag="VGE59_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY1075025.1"
                     /translation="MVRIAGTNLNNEKRIEIALTGIFGIGPTTSTKILEACNIQGNPR
                     TKDLTDDEVERIRAYIDKNLTVESDAKILITRNIKRLKDISAYRGLRHNAGLPVHGQR
                     TKTNARTKRGKKVTMGSGRKKAAAKT"
     gene            810..1196
                     /gene="rpsK"
                     /locus_tag="VGE59_05055"
     CDS             810..1196
                     /gene="rpsK"
                     /locus_tag="VGE59_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY1075026.1"
                     /translation="MAQVTSRRKKKITNVPKGRIYVQSTFNNTIITLTDSNGGVLGWE
                     TAGHSGFRGARKSTPYAAQVAMKSVIDKAQTFQVEEVDVYVKGVGSGRESAVRALQGS
                     GITVTGIKDITPIAHNGCRPKKPRRV"
     gene            1215..1766
                     /gene="rpsD"
                     /locus_tag="VGE59_05060"
     CDS             1215..1766
                     /gene="rpsD"
                     /locus_tag="VGE59_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY1075027.1"
                     /translation="MANTPVKPQTAYSSDKPAAAAAPRPSAPRGRKVSDYGRQLAEKQ
                     KARNEYGLRETQFHRYFEKAHKSSVATGQALFTSLETRLDNVVFRAGFAKSRAMARQM
                     VGHGHVFVNGKRVNMPSYPVKAGEVINLKKAETFEHNKEAILPDWLSYSSKTKSATVE
                     RMPKADDIVTDINSQLIIEFYSR"
     gene            1830..2648
                     /locus_tag="VGE59_05065"
     CDS             1830..2648
                     /locus_tag="VGE59_05065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY1075028.1"
                     /translation="MAYILPDTELLTIKQVAETGVNQATFEIEPLSPGYGVTVGNTLR
                     RILLSSMEGAAITSVRIDGATHEFTTVPGVHEDMVNLILNMKQVKLRLHGNEPTSLVL
                     TKKGPGAVTVADFKANSAVEFINPEQVIANLDKGAQLNLEIEVRKGRGYSPVEARRDE
                     KLPLGTIAIDSIFSPVIRVTYDIENTRVGNVTNYDKLIMTVITDGSISPQDALQTAAK
                     IAVEHFSAVAGITTNLAETETVVATAMEVTVDEDAVVAAPKKRASRKTATKEEE"
     gene            2648..3097
                     /gene="rplQ"
                     /locus_tag="VGE59_05070"
     CDS             2648..3097
                     /gene="rplQ"
                     /locus_tag="VGE59_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY1075029.1"
                     /translation="MNKSSFGRKRDQRDILLRNLATSVILYESVVTTEAKGRTVQPLV
                     ERLIRIAQKEDKLSARRELMTYIFDEKAIHKLFEEITPRYTDRTSGFTRRFRLPARPG
                     DGAARMVVQLTKSVRFEEPVTAKASKEEKKVAPSETKEVTTEGESHE"
     gene            3090..3476
                     /gene="rplM"
                     /locus_tag="VGE59_05075"
     CDS             3090..3476
                     /gene="rplM"
                     /locus_tag="VGE59_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY1075030.1"
                     /translation="MNKKPINRAVVDRAAVTISARGKVLGRLATEVATILRGKDSVHF
                     ESHLLSGRPVIVTHAKDVIVTGNKLEDKKYYRHTGYLGNMKTETLSDMMKTKPDEVIR
                     HAVRGMLPDNRLRQHWLAALEIREEE"
     gene            3481..3867
                     /gene="rpsI"
                     /locus_tag="VGE59_05080"
     CDS             3481..3867
                     /gene="rpsI"
                     /locus_tag="VGE59_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY1075031.1"
                     /translation="MAKTETSNAYFTGVGRRKTAVAQIRVMSGSGQFTFNGEKTDPHH
                     AWVRPLDLVGKKDSTDVSIVVRGGGFASQDDAISLGIARALVTMDDTLKPTLRKAGLV
                     TVDARVKERKKPGLKRARKAPQWSKR"
     gene            3945..4661
                     /locus_tag="VGE59_05085"
     CDS             3945..4661
                     /locus_tag="VGE59_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1075032.1"
                     /translation="MNTATFHFPLVRKRQIAENTFEFTFNTQNTDYLFRPGQYAYVSL
                     IHHNLGDQSRIFSFVNAPRPTGEVIIAARIRAQSDYKQALAHLQPGEVAEFSRALGFF
                     DLPANKGIPIVMMAGGIGIVPFLSLLESEAHAASTRPIELYISHQSPESEVYMKEIKG
                     LAPRLPNLILHQTYTQKYPTAWHGKKGRFTTDDFSFQLNTHFYVAGTPAMTDHIISQL
                     RSLDIPLSHIFMEAFSGYEI"
     gene            4735..5049
                     /locus_tag="VGE59_05090"
     CDS             4735..5049
                     /locus_tag="VGE59_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075033.1"
                     /translation="MAVSEDIAEIVTCPWTRERLQELVTALEELSEENQAILKKHLVA
                     FAQLERKLSPDAFWFQVCRDSADFRETEGAIDEVCNIVDPVIDLMRTSREVCTWIQRT
                     MQ"
     gene            complement(5629..6744)
                     /locus_tag="VGE59_05095"
     CDS             complement(5629..6744)
                     /locus_tag="VGE59_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075034.1"
                     /translation="MMHSRTYRILIIVAIVSLVITSATLAYMRRIDASTFNLTQQTNT
                     TLYQTEKIRQFELEDSIAAAGAQLAQKEAEVKAKQEELEAKQAELAKAETERLAKVKE
                     LESAQKKIKDQQSQISSTASELKRLSDRPPLFSFKVESSTLASVEQKKNDVKEVVTAA
                     YDLIGDIYGKPYLLKAVTISFVDSFSSPSAAAEIVITNGSEGLDVTIKLKDFDKNSFN
                     DVNSIIHEVVHAYHGIAILEPVAYEEGITVAATDVVMAQLIAQGKIPSFKPLYIRLAS
                     SEYATASSLPSNAATLYGSPQVAHYYQVAGYGWYQLYKADSNFFKNFNDRLYSYKRDG
                     NELTESLVKEVIKQAYSGPVQGKSIGSWLETAAFKLQ"
     gene            6821..7162
                     /locus_tag="VGE59_05100"
     CDS             6821..7162
                     /locus_tag="VGE59_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEY1075035.1"
                     /translation="MNITRHQQLLSLIKRVTSAILRELDHEHLHSAVITNVILSNDGR
                     EARIWVDAPAATITLLNTKYRGEIQHLFMKQYARKVVPRLTFEKDTGEIDRLEVLLSE
                     EPLQDEPQSDL"
     gene            7140..7523
                     /locus_tag="VGE59_05105"
     CDS             7140..7523
                     /locus_tag="VGE59_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075036.1"
                     /translation="MNRSQIFSAVSISASALAVWIFIVTQVQPDKNDPLIISTFFLSL
                     AVWLGSLLAAIIYTMKVRKNNREVIYAHIRPSIRQGLLIATSLSTLLFLQMIRVLSVW
                     DAILVIFIVILFELALRQNDPMTKA"
     gene            7526..8218
                     /gene="pheS"
                     /locus_tag="VGE59_05110"
     CDS             7526..8218
                     /gene="pheS"
                     /locus_tag="VGE59_05110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY1075037.1"
                     /translation="MQKGSLHPLTQIQRIAVSALGDMGFDIVEGPEIDTAWYTFDSLR
                     LHADHPARSDQDTFWLTDGKLLRPHTSNMQLHVAQQKNLPIRAVYFGNCYRNDATDAT
                     HEIVFRQIECLAIDETITLQNLLATLETFIKRVLGPETTYRFRPHNFPYTEPSIEVDV
                     LHNGKWVELLGAGMVHPEVLRNMGIDSNVYSGFAFGIGIDRLALMKWGVEDIRLLRSN
                     RLPFLRQFRSNS"
     gene            8215..>9236
                     /locus_tag="VGE59_05115"
     CDS             8215..>9236
                     /locus_tag="VGE59_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEY1075038.1"
                     /translation="MKYLYSWLKELYPTIPGLGELESLLIQLGHDVESITPVTYDGVI
                     VAQIQTIEKHPNADRLSLVTITTGHTTHKVVCGAPHLTVGQKVAYAPVGTTLHCGLTL
                     RPISIRGEASEGMLCAPDELGIGTSHEGLLTLPDTATAGEPLDEYAPQDAIISLDITS
                     NRGDVLSHFGLARDLKAGAEQKLLTPSFLSIQTEAPTEAEIGEIHSDVTAFSLASTTD
                     VKSSTTPLYMQSRLLLLGQKPINLATDITNYLQLEYGQPLHAYDAKKLQAPIQISVRR
                     AHQDERIQLLNGKSTTLSPQSLVISSHEHAVALAGVMGGEETKVTKETTDVLLESAHF
                     HAKAVT"
BASE COUNT         2643 a         2218 c         1983 g         2392 t
ORIGIN      
        1 aatcagtagg tacatggaag aaaaagaatt tgtagaagtc caaggcacgg ttactgaagc
       61 cctgccgaac cgaatgttcc gcgttaagct tgataacgaa gctatggtat taggaatact
      121 atcgggtcaa atggcgcgac ataaaattcg tgttcttctg ggagaccgag tcacactcga
      181 gctcccgatc attgatctta ccaaaggccg tattatacgg cgtctcagtt aaagcacgag
      241 gtacacctaa tgaaagtacg tccatctgtc aaaaaaatgt gcgatacctg ccagattatt
      301 cgtcggaagg gtgttgttgt cgtgctctgc aaaaaaagtc cgcgccacaa acagcgccaa
      361 ggttaaataa gggtaattat ggtacgtatt gctggtacaa atttaaataa cgaaaagcgc
      421 attgaaattg ctttaaccgg tatcttcgga atcggaccaa ccacctcaac taagattctt
      481 gaggcctgca atattcaagg gaacccccgc acgaaagacc ttaccgacga cgaagttgaa
      541 cgcattcgtg cctatattga taaaaatctg actgtcgaaa gcgatgcaaa gattcttatt
      601 acccgtaaca ttaaacgatt aaaagatatt agcgcctatc gtggtcttcg ccacaacgca
      661 ggtcttcctg ttcatggcca acgaacaaaa accaacgcac gtaccaaacg tggtaagaaa
      721 gttacaatgg gttcgggtcg caaaaaagct gccgctaaaa cctaataacg aaacagcgtt
      781 acactaaaaa gattgaagta aaggaacgta tggctcaagt tacgtcacgt cgcaaaaaga
      841 aaatcacaaa tgtccccaaa ggccgtattt acgtccagtc cacttttaat aacacgatta
      901 ttaccttaac cgatagtaat ggtggcgtac ttggctggga aactgctgga cactctggtt
      961 ttcgtggcgc ccgtaaatcc accccatacg ctgcccaggt cgcaatgaaa tcagtcatcg
     1021 acaaagctca gactttccaa gtcgaagaag ttgacgtcta cgttaaaggc gttggatcag
     1081 gacgagaatc agctgtccgc gccctccaag gcagcggtat cactgttact ggcatcaaag
     1141 acattacgcc aattgcccac aatgggtgcc gtcctaaaaa gccacgtcgg gtctaaccca
     1201 agcaaggaaa ttgtatggca aatacaccag tcaaaccaca aactgcatat tcttccgata
     1261 aaccagccgc tgccgctgcc cctcgtcctt ctgcccctcg tggccgtaag gtgagtgact
     1321 atggccgtca gcttgctgaa aaacaaaaag cacgtaacga atacggctta cgagaaactc
     1381 agttccaccg atactttgaa aaagcccata aatcatcggt agcaaccgga caagcacttt
     1441 tcacatcatt agaaacacga cttgataatg ttgtattccg tgctggattt gccaaaagcc
     1501 gcgctatggc acgacaaatg gttggtcacg gccatgtctt cgttaacggt aaacgtgtta
     1561 acatgccaag ctatccggtt aaagccggtg aagtgatcaa cctcaaaaag gctgagacct
     1621 tcgagcataa taaagaagca attttacctg actggttgag ctactcgagc aaaacgaaat
     1681 ccgctacggt cgaacggatg ccaaaagccg acgatatcgt cactgatatc aactcacagc
     1741 tcattattga gttctacagc cgctaataaa aatatttttc tggacgaatc ctcgtggctg
     1801 tacgaggaga tgacagagaa aggactacca tggcctacat tctacctgat actgaattac
     1861 taacgatcaa acaagtcgct gagactggtg taaaccaagc cacctttgag attgagccac
     1921 tatcgccagg ctacggcgta acggttggca atacgcttcg tcgtattctt ttaagctcaa
     1981 tggaaggcgc cgccattact tcggttcgca ttgacggtgc aactcatgaa tttactaccg
     2041 ttcctggcgt tcatgaagat atggtcaacc tcatcttaaa catgaaacag gttaagctcc
     2101 gacttcatgg caacgaacca accagccttg tcttaaccaa aaaaggccct ggtgccgtaa
     2161 ccgtagctga ctttaaagcg aattctgctg ttgaatttat caaccctgaa caggttatcg
     2221 cgaaccttga taaaggcgct caacttaact tagaaattga agtgcgtaaa ggacgtggat
     2281 acagcccagt tgaagcacgt cgtgacgaaa aactgcccct tggcactatt gctatcgaca
     2341 gcattttctc acccgttatt cgcgtcactt atgacattga gaatacccgc gttggcaatg
     2401 ttacgaacta cgacaaactc atcatgaccg ttatcaccga tgggtcaatt agcccacagg
     2461 atgcactcca gacagctgcg aaaattgctg ttgagcattt ctctgccgta gcaggcatta
     2521 ctacgaattt agccgaaaca gaaaccgttg ttgcgaccgc catggaagta actgttgatg
     2581 aagatgccgt tgtcgctgct cctaaaaagc gagcaagccg caagactgcg actaaagagg
     2641 aagaataatg aataaatcgt cttttggaag aaaacgcgat caacgcgaca ttttactgcg
     2701 caacttagcg acttccgtta ttctttatga atctgttgtg acgaccgaag caaaaggtcg
     2761 gacagttcaa ccactcgttg aacgtctgat tcgtattgct caaaaagaag acaagctttc
     2821 agctcgtcgg gaacttatga cttacatttt cgacgaaaag gcaattcata aattattcga
     2881 agaaatcaca ccgcgttaca ctgatcgcac atccggcttt acccgccgtt tccgtttacc
     2941 agcccgtcca ggagatggag ccgcgcgtat ggttgttcag ctcacaaagt ctgtccgttt
     3001 tgaagaaccc gttacagcga aagcttcaaa agaagaaaaa aaagtagctc cttccgaaac
     3061 caaggaagtc actactgaag gtgaaagtca tgaataagaa accaattaac cgcgccgtcg
     3121 ttgatcgcgc agccgtcact atctcagctc gtggaaaagt tctcggtcgg cttgctactg
     3181 aagttgctac tattttacgt ggcaaggact cggtccactt cgaaagtcac cttttatcgg
     3241 gccgccctgt aattgtgaca cacgccaaag atgtaatcgt taccggtaac aagctcgaag
     3301 ataaaaaata ttatcgacac accggatacc tcggcaacat gaaaactgaa acactcagcg
     3361 atatgatgaa aaccaaacca gatgaagtaa tccgacatgc cgttcgtggt atgctccctg
     3421 ataaccgact tcgtcagcac tggttagcag cactcgaaat ccgggaagag gaataagaat
     3481 atggcaaaga cagaaacctc aaacgcctac tttactggcg taggacgccg taaaaccgcc
     3541 gtagcgcaaa ttcgcgttat gtcaggatca ggacaattca cttttaacgg cgaaaagacc
     3601 gatcctcatc atgcatgggt tcgtcctctt gatttagtcg gcaaaaaaga tagcaccgat
     3661 gtttccattg tggttcgtgg tggtggattt gcaagccaag acgacgctat ttccctcggt
     3721 attgcccgtg cgttagttac catggacgac acgctcaaac caacactccg caaagctggt
     3781 ctcgtcaccg tcgatgctcg tgtcaaagag cgtaagaaac caggtctcaa gcgtgcccgt
     3841 aaagcaccgc agtggtcaaa acgttaaaga tcaagaaatt tcttgtaaca aaaaaccgtc
     3901 cttgtgtggc ggttttttgt tactgaaggt atgatcacag tgttatgaat actgctacat
     3961 tccatttccc cttagtaagg aaacgtcaaa ttgctgaaaa tacatttgag tttactttta
     4021 atactcaaaa tacagactat ctctttcgcc cagggcaata tgcatacgtg agcctaatac
     4081 atcacaatct tggggatcag agccgtatct tctcttttgt taacgcccct cgtccgactg
     4141 gagaagtcat tatagcggca cgtatccggg cacaaagcga ttataaacaa gcccttgcac
     4201 accttcagcc tggtgaagtt gcagagttca gccgagcact cggttttttt gatcttcccg
     4261 cgaataaagg gataccgatt gtaatgatgg ccggaggaat tggtatcgta ccgtttttaa
     4321 gtcttcttga atctgaggcc catgccgctt cgacccgacc aatagagcta tatatcagtc
     4381 atcaatcacc cgaatcagaa gtatacatga aagagatcaa gggtcttgct cctcggttac
     4441 cgaatctcat actccaccaa acgtatacgc agaagtatcc tacagcgtgg catggaaaaa
     4501 agggaaggtt cactaccgac gacttttcct ttcaactgaa tactcatttt tatgttgctg
     4561 gtacgccggc catgaccgat catattattt cccagctacg ctcactcgat atacctctct
     4621 ctcatatttt tatggaggct ttttcaggat acgaaatata attgacgaaa atcgcaaaat
     4681 actctatact accattactt tctcaatccg catgaagaag gggggtgata cacgatggcg
     4741 gtgagtgaag acatcgcaga gatagtaacg tgtccttgga caagagaacg actccaagaa
     4801 cttgtcactg ctctggagga gctgagcgaa gaaaaccaag ctattttgaa gaaacatttg
     4861 gtcgcattcg cacagctcga aaggaagctg agtcccgacg ccttctggtt ccaggtttgt
     4921 cgtgattcag cggacttccg tgagaccgag ggtgctattg acgaagtctg taacatcgtc
     4981 gatcccgtta ttgacctcat gcgaaccagc cgagaggtct gcacctggat ccaacgcacg
     5041 atgcagtagg agtatcatgc atctgtcctc accctgaagc ccggcattct ctcgagggaa
     5101 aatgccgggc ttctcatata gttttataat tccactgctt gacaggccat tccttggcag
     5161 gctatgctga gtataagcaa taaggaggga cttttttcta ccaacttatt gcattaacaa
     5221 agtctgatgc aggtaatagt agcctataat gtggcagaac cgtacagttc tcgcctgtgt
     5281 aatgtataat caagctactc caggcctccc agactgcaca tcatattcgc taggtgggat
     5341 tctgattgcg caaagaattc cagaggacaa tgttatatgg gtcgtagtca gtggacgcgg
     5401 tcgcgtaaca tgaattgcgt gccttcctct ggcacaaaag aatgcctttc cccggcacct
     5461 ccgatgcaaa acggatgagc agttcgctcc acgggtaagg ttccagtcga cggaagccga
     5521 acggtttgca acgttcggct tttcttttta ttttccttct ttgaaggaaa ataaaacttc
     5581 gaacaaaact gagaagtctt tcttatatta cttagaacta attcgatact attgcaactt
     5641 aaaagcagcc gtttcaagcc acgaaccaat ggatttacct tgaacaggcc cgctatacgc
     5701 ttgtttaatc acttctttca cgagtgactc tgtgagttca tttccatctc gtttatatga
     5761 gtaaagtcgg tcgttaaaat ttttaaaaaa gtttgaatcc gctttataga gttgatacca
     5821 gccgtaccct gcgacttgat aataatgcgc gacttgagga gacccataca gcgttgcagc
     5881 gttgctaggc aacgaggacg cagtggcata ttcactagaa gctagacgaa tatagagtgg
     5941 tttaaaactt ggaatctttc cttgcgcaat gagctgtgcc atgacgacat cagtggcagc
     6001 gacggtaata ccttcttcat aggccacagg ctctagaata gcaataccat gatatgcatg
     6061 aacgacctcg tgaataatcg agttaacgtc attaaaacta tttttatcaa aatctttgag
     6121 cttaatcgta acatctaacc cttcactccc gttggtaata actatttcgg cagcagctga
     6181 cgggcttgaa aagctgtcaa cgaatgagat agtaacggct tttaaaaggt agggtttccc
     6241 atatatgtca ccaataagat catacgctgc ggtgacaacc tcttttacat cgtttttctt
     6301 ttgctctaca cttgcaagcg tacttgactc aactttaaag gagaaaagag gaggacgatc
     6361 actcaatcgt ttaagttcag atgcagttga agaaatttgt gattgctgat ctttaatttt
     6421 cttttgagcg ctctcaagtt ctttcacttt ggcgagacgc tcagtttcgg ctttggctaa
     6481 ttctgcttgc tttgcctcta actcttcctg ttttgccttt acttctgctt ctttttgagc
     6541 caattgagcc cccgcagctg caattgagtc ctccaactca aattggcgaa ttttctctgt
     6601 ctgatagaga gttgtattcg tttgctgtgt gagattaaac gtacttgcgt cgattcttct
     6661 catatacgcc aaagtagcac tggtgataac caggctcaca attgcaacga ttataaggat
     6721 gcgataggta cgagagtgca tcattattta ccataacatt gacctactgg agagacaacg
     6781 tccgttgagt cttgggtcac gaatatggta tgatagacaa gtgaatataa cacgacacca
     6841 acaactgctc tcactcatta aacgcgttac aagcgctatt ttgcgtgagc tagaccacga
     6901 acatctccat agtgccgtta ttaccaacgt gatcctcagt aacgacggtc gggaagctcg
     6961 tatttgggta gatgctcctg ctgcgactat tactcttttg aatacaaagt atcgaggaga
     7021 aatccaacac ctctttatga aacagtacgc tcgaaaagtt gtaccacgat taactttcga
     7081 aaaagacacc ggggaaattg accgcttaga ggtactgtta agtgaggagc ctttacaaga
     7141 tgaaccgcag tcagatcttt agcgccgtta gcatttctgc ctctgcgctg gccgtgtgga
     7201 ttttcattgt gacgcaagtt caacctgata aaaacgaccc tctcattatt agtaccttct
     7261 ttctctcgtt agccgtatgg cttggcagtc tcttggccgc gataatttat accatgaaag
     7321 ttcgtaaaaa taaccgagaa gtgatatacg ctcatatccg gccatcaata cgacaaggac
     7381 tattgattgc cacctccctc tctactctct tatttttaca aatgatccgt gtcctatcgg
     7441 tatgggatgc tatcctcgtt attttcattg ttattctctt tgagctagcc ctccgacaaa
     7501 atgatcctat gacaaaggcg taaacatgca aaaaggctca cttcacccct taacacaaat
     7561 acaacgtatt gccgtctcag cgctcggaga tatgggcttt gatattgtag aaggtccgga
     7621 aatagataca gcctggtaca cgtttgatag tttacgtctc catgccgatc atcccgcccg
     7681 ctcagaccaa gataccttct ggctcacaga cggcaaattg cttcggcctc atacttcgaa
     7741 catgcaactt catgttgccc agcagaagaa cctccccatc cgagcggtct actttggaaa
     7801 ttgctatcgt aacgacgcca ccgacgctac tcacgaaatt gtcttccgac aaattgaatg
     7861 cctcgcaata gacgaaacta tcacgttaca aaacttactc gccacactcg aaacattcat
     7921 taaacgtgtt ctcggcccag agacaacata tcgtttccga cctcataact ttccttatac
     7981 ggaaccaagc attgaggtcg atgtccttca taatggaaaa tgggtcgagc ttcttggcgc
     8041 gggcatggtt catccagaag ttttgagaaa tatgggcatt gattctaacg tatatagcgg
     8101 ctttgctttt ggtattggaa ttgatcgact ggcccttatg aaatggggag tcgaagatat
     8161 tcgtctttta cgaagtaacc gcttaccatt tttacgtcaa ttccggagca actcatgaaa
     8221 tacctttatt cttggctcaa agaactctat cctacaattc ccggtttagg agagcttgaa
     8281 tctcttttaa tccaacttgg acacgacgta gagtcaataa cgccagtaac atatgatggt
     8341 gttatcgtgg cgcagatcca gactatcgag aaacatccaa atgctgaccg tttgtcactt
     8401 gtgactatca caacagggca cacgacccac aaagttgtgt gtggggcgcc tcacttaact
     8461 gtcggacaga aagttgctta cgcgcctgtt ggtacgaccc ttcactgcgg cctgacgcta
     8521 cggccaattt ctattcgagg agaagcttct gaaggaatgc tctgcgcccc agacgagctt
     8581 ggcattggca cttctcacga ggggctactg accttaccgg ataccgctac tgccggtgag
     8641 cctctcgacg aatatgctcc ccaagacgct attatttcat tagatataac atcaaatcgc
     8701 ggcgatgtgt tgtctcactt cggacttgca cgagatctta aagcgggagc ggaacagaag
     8761 ctgcttaccc cttcatttct tagtatacag acagaagcac cgaccgaagc agaaatcggc
     8821 gagatccatt cggatgtcac tgctttttct ttggcaagta caacagatgt aaaatcaagc
     8881 acgacgccgc tctacatgca aagtcgccta cttttacttg gtcaaaaacc cattaatctt
     8941 gctacagata tcaccaacta tcttcaactt gaatatgggc aacccctcca cgcctatgac
     9001 gcgaagaaac ttcaagcacc tatccaaatc agtgtccgac gcgcgcacca agatgagcgt
     9061 attcagctcc tgaatgggaa atctacaaca ctttctccgc agtcccttgt tatttcatcg
     9121 cacgaacatg cggtagcgtt agcaggagtt atggggggag aggaaacaaa agttaccaag
     9181 gaaactacgg acgtattact tgagagtgct cactttcatg ctaaagcagt aacaat
//
LOCUS       DASWYB010000091         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_101357, whole genome shotgun sequence.
ACCESSION   DASWYB010000091 DASWYB010000000
VERSION     DASWYB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101357"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VGE59_05120"
     CDS             <1..679
                     /locus_tag="VGE59_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1075039.1"
                     /translation="LCFVTMTDYGKDVTSIVAYEPQSIEATLASAVCAADMNQAHIAE
                     TEKYPHATYFLNGGKEDTHAKEEHVLIPSNKHVKTHDEAPEMKAKEICDAAIERLATN
                     DFIFVNFANPDMVGHTAKKEAIITAVETVDRELGRLAEATLKANGAVIAIADHGNAER
                     MIDPETGAPHTAHTTNLVPCILIADSHAHVSLAQGGGLKDIAPTVLDLLGIEKPASMT
                     GASLITE"
     gene            complement(763..1125)
                     /locus_tag="VGE59_05125"
     CDS             complement(763..1125)
                     /locus_tag="VGE59_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075040.1"
                     /translation="MQRGAYQDTFRSPRDLVHALMGLGVDFYVVVSGGGSVSLLQHDD
                     PIAAVTQQLAEYSRRLPRSCHFEARSTSVYFRSFDCTIEIRREPEGPLVLRVRRFGAD
                     GRVNPRFAPVGWQFVPEA"
     gene            1232..2638
                     /locus_tag="VGE59_05130"
     CDS             1232..2638
                     /locus_tag="VGE59_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY1075041.1"
                     /translation="MLPEKIALVDVETSGARVTRDRIIEIGILRIENGVLVKTYQSLI
                     NPGGYLSPFIEQYTGITREELVGAPKFEDIAHEIEAMLDDCVFVAHNVRFDYGFLKNE
                     FRRLEKQFAPKHFCTVKLSRALYPQHNRHGLDALIERFNIVCPNRHRALDDAKVMWEF
                     LQISQQLIPQEKFAAEIKRALKQPSLPSQLSKDSIDNLPEGPGVYIFYGEEGYPLYIG
                     KSKNIRERVLSHFSIDHTSSREMHMCQQVASIETIETSGELTALLRESQLVKQMQPLY
                     NRKLRHSRSLVLLRKTSTPDGYETATIESTPHIQTEDLDSILAVCKSVRQAKDLLQST
                     AQTYGLCHKLLGLEKGSGPCFGSQLGTCSGACQGKEPPVRYNFRIMNAFIASKLQAWP
                     FDGPILIFEDEGDGTQALIVDNWCVVGTMNESNQADFTPSEYAFDLDTYKILRQFLVT
                     SKKKLRIKPFEQMAMAEI"
BASE COUNT          762 a          726 c          597 g          635 t
ORIGIN      
        1 cctctgcttt gtgacgatga ctgattacgg caaagacgtc acttcgatcg tcgcctacga
       61 gccacagtct atcgaagcaa cattagcaag cgctgtctgc gcagccgata tgaaccaagc
      121 tcacattgcc gagaccgaaa aatacccgca cgccacctat ttcttaaacg gcggaaaaga
      181 agacacacat gcaaaagaag agcacgtact cattccaagt aacaagcatg taaaaaccca
      241 cgacgaagca ccagaaatga aagccaaaga aatttgcgac gccgcaattg aacggctcgc
      301 taccaacgac tttatcttcg tcaactttgc caacccagac atggtaggac acactgctaa
      361 aaaagaagca attattaccg ctgttgaaac agttgatcgc gagcttggcc gcttagccga
      421 agcaaccctc aaagccaacg gtgctgttat tgccattgcc gaccacggga acgccgaacg
      481 catgatcgac cccgaaacag gcgcaccaca cacggctcat acaaccaacc tcgtgccatg
      541 tattctgatt gctgactctc atgcccatgt gtcactggca caaggcgggg gactgaaaga
      601 tatcgcccct acggtgttgg acttgcttgg aatcgaaaaa ccggcctcta tgaccggtgc
      661 gtcacttata actgaataaa agagttagta taaaaagaag tggtcggctt acgctgaagc
      721 cgaccttcta ttcttcatga agttcccctt tgaacctttt ccctacgctt caggcacgaa
      781 ttgccatcct acgggtgcaa accgcggatt gactcttcca tctgcgccaa agcgacgtac
      841 tcgtaggacg agtggccctt ccggctcacg ccgaatctct attgtgcaat caaatgaccg
      901 gaagtacaca gacgtagacc gcgcctcaaa gtggcaacta cgtggtaatc gccgactgta
      961 ctccgctagc tgctgcgtca cggccgcaat cgggtcgtcg tgttgaagga gactcacaga
     1021 gccaccccca ctcacaacaa cgtaaaaatc cacccctagt cccatcagag cgtgaaccag
     1081 atcacgcgga cttcgaaagg tatcttggta ggccccccgt tgcatagtat caaccctccc
     1141 taattgagta ttatagttgg gacaaagcag gcgtacaagc gcatactttg tcattgacac
     1201 ttcggcaaat tatcggtagc gtgaggaaca tatgttgcca gaaaaaatcg cccttgttga
     1261 tgtcgaaacc tccggtgcgc gtgtaacgcg agatagaatt attgagattg gcattctccg
     1321 aattgagaat ggcgtacttg ttaaaacgta tcaatcgctc attaatcccg gcggttatct
     1381 ttcacccttc attgagcagt acactggcat tacacgtgaa gaattagtcg gcgctccaaa
     1441 gtttgaagac attgcgcatg agatagaagc tatgctcgac gactgcgtgt ttgtcgctca
     1501 taacgttcgg tttgattacg gatttttaaa aaacgagttt cgacgcttag aaaagcagtt
     1561 tgccccaaag catttttgca ctgttaagct ttctcgcgca ctttacccac aacataaccg
     1621 gcacggactc gacgccttga tcgagcgatt taatattgtc tgtcccaacc gtcatcgtgc
     1681 cttggacgat gccaaagtta tgtgggagtt tttgcagatt tctcagcaac tgattccgca
     1741 agaaaagttt gctgcggaaa ttaaacgtgc cctcaagcaa ccttcactgc caagccagct
     1801 cagcaaagac tcaatcgaca atttgcccga aggtcccgga gtttacattt tctatggcga
     1861 agaaggttac cctttatata ttggcaagag taaaaatatt cgtgaacgcg ttttatcgca
     1921 cttttcgatc gaccatactt cctcgcgtga aatgcacatg tgccaacaag tggcctcgat
     1981 agaaacaatc gaaacgagcg gcgaactcac cgccttgctc cgcgaatccc aacttgtgaa
     2041 gcaaatgcag ccgctgtata accgcaagct ccgccattcg cgtagtttag tcctgctacg
     2101 aaaaacaagt acaccagatg gctacgaaac agcaaccatc gaaagcacgc cccatatcca
     2161 gaccgaagat ctcgactcta ttttagctgt ctgcaaatcc gttcgccaag ccaaagactt
     2221 attacaaagt accgcccaaa cttatggttt atgccacaag ctgcttgggc tcgaaaaggg
     2281 gagtggaccg tgttttggtt cacagcttgg tacctgcagc ggtgcctgcc agggcaaaga
     2341 accgcccgta cgttataact tccgtattat gaacgccttt attgcatcca agctccaagc
     2401 ctggccattc gatggaccta tcctcatttt cgaagatgaa ggcgacggca cccaagcttt
     2461 gattgtcgac aactggtgcg tggtcggtac tatgaacgaa tcaaaccaag ccgattttac
     2521 cccaagcgaa tatgcctttg atttagatac gtataaaatt ctgcgccagt ttttagtaac
     2581 ctccaaaaag aaactccgca taaaaccgtt cgaacaaatg gccatggctg aaatataaga
     2641 gaagagagca catctctgcg ctctcttctg cctgtagaac tgctattccc tcttagcaag
     2701 agagggctac ttccaaacgg
//
LOCUS       DASWYB010000092         9243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U3700
            k141_1016114, whole genome shotgun sequence.
ACCESSION   DASWYB010000092 DASWYB010000000
VERSION     DASWYB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959508
            Sequence Read Archive: SRR11449186
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 04:44:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,033
            CDSs (total)                      :: 980
            Genes (coding)                    :: 977
            CDSs (with protein)               :: 977
            Genes (RNA)                       :: 53
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016114"
                     /isolate="SMAG_U3700"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.65 N 116.53 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VGE59_05135"
     CDS             <1..789
                     /locus_tag="VGE59_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY1075042.1"
                     /translation="EQVVEQIIRPTGLIDPKLTLHQTADQVAHLLENIKLRVAKNERT
                     LVTTLTKKMAEDLSEYFIGQGLKVRYLHSDVDTIERIEILRDLRKGIFDVLVGVNLLR
                     EGLDLPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVDGEVILYADVMTGSLQRAI
                     QETERRRAIQIAYNEKHGITPQSIQREIVSIVNREIEGEETHKLDQVEREMARGNLDA
                     VIKQKEREMKAAAKDLQFELAALLRDELGELRKLQRQQVSNLLS"
     gene            complement(802..2118)
                     /locus_tag="VGE59_05140"
     CDS             complement(802..2118)
                     /locus_tag="VGE59_05140"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY1075043.1"
                     /translation="MIHLPLSKDPGIFTHTTLSNGISIYYKQAEQLFGLPKIACCALV
                     TVGGRDDPRGKEGTAHFFEHMPFRGTKHFPTLAELTQDIENNGGYVNAFTSDEVTGYE
                     VIAPTSMIEGAIARVADMFLYPILRPEDIEIERSVILEELRNKHAHVNFFVRSKVMEG
                     LLGNHPLVYATIGTEQALNSIAQEDLVAFHEKYYNSHNLTLFFTGDFKSDELLSHCET
                     FFGSITHKGEKTERNLELKPHSIVEPVQTYTPSEYNRSVYALGRVLPKADLRIATILK
                     LYTDMLTRGFTSPLYQEIREKRGLAYSMGMHAGIYQDLTLLMFTISTQYRYMDQVDTI
                     AWEQMRAILSNDERFQEVKHSIKQSILHREYSVGSLNDEAVDDFQDYGRIIPLTEYVA
                     LLDSIQLQDVQDFIEPLLVKEEFLNIRVNCDRQGLSESTTTTENVE"
     gene            2244..5117
                     /gene="uvrA"
                     /locus_tag="VGE59_05145"
     CDS             2244..5117
                     /gene="uvrA"
                     /locus_tag="VGE59_05145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY1075044.1"
                     /translation="MQDDKIIIRGAREHNLKNIDLELPRNSLIAFTGLSGSGKSSLAF
                     DTIYAEGQRRYMESLSSYARQFLGQMEKPDVDSIDGLSPAISIDQKSTSHNPRSTVGT
                     ITEINDYLRLLYARIGVPHCPVCNRIISRQPTEQIVDTILRYPESSRVLILAPVVRGR
                     KGEYHQLLYDMYHGGFARVRVNGEIRSLSDSIQLDRYKAHDIDIVIDRLTIGHVMTSR
                     VQEAVEQALKLTKGLVTILNETTGEEVILSQQFACPVDGTSIEELTPRSFSFNSPFGA
                     CETCNGLGYIRELDATLLMPDTSKSIQDGAIMPWHYRGNNYYGSIIKSIAQHYNISLT
                     QPLNKLDKKKLDTIIQGAAEPVLIPATYMTSTGPKRFSIRFQGLMNHLQKRYQETESD
                     AVRSDIERYMAAKQCATCQGQRLRHNSLLVTIYDKNIAEIGEFTIEEALNWFRGLKLN
                     DKEMQIALQIVFEITNRLEFLYKVGLNYLTLSRSAVTLSGGEAQRIRLASQIGSALTG
                     VLYVLDEPTIGLHQRDNTRLIETLKTLKDRGNTIIVVEHDEDTIRASDYVVDIGPGAG
                     EHGGNVVAAAERSAFLADDSSITARYLSGKELIPTPEKRRQANKLREVTVHHARENNL
                     KDITVSFPLGLMTCVTGVSGSGKSTLVNEILYKGLTNIVNRTNHPVGKHDKISGAEEV
                     ERVIIIDQSPIGRTPRSNPATYTKSFDHIRDLFSRAPEAKIRGYKPGRFSFNVDGGRC
                     DNCKGDGVIRIEMHFLADVEIPCEVCKGTRYNRETLEVRFKGKNISQVLNMTVDEGVE
                     FFSSIPSLHDTLLTLQAVGLGYIRLGQSATTLSGGEAQRVKLAAELGKRTHGKSLYIL
                     DEPTTGLHFEDIRKLLEVLNRLVNKGNTIVIIEHNLDVIKNADWIIDLGPEGGVQGGT
                     IVAKGTPEEVAEVSSSYTGHYLRIHLDQAKQYKKIALKTRAKA"
     gene            5126..6727
                     /gene="uvrC"
                     /locus_tag="VGE59_05150"
     CDS             5126..6727
                     /gene="uvrC"
                     /locus_tag="VGE59_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY1075045.1"
                     /translation="MTEHIKEKLRHLPHSPGVYHYFDSQQNLLYVGKAKDLFKRVHSY
                     WNKQHQSPWTQLLVEEIDDIKIIEVKTELEALMLENSLIKSLRPKYNIQLRDDKTFPF
                     LRVSREDFPTFGITRKVKEDGARYFGPYLSASYLRSMLKLLQSLYGIKTVSEKSYESR
                     SSVPNQIGLGARNLENREAYNQNVKEAIRFLSSPQPEMEREIKQAMDTAAHNEEYERA
                     AILRDRLGAVQQLRQNQSLFSPKGLSTDYIGWTQAGKLITVYVLMEREGKILDKRDFV
                     FEFPSPLTPNELTDEILDYLYIQGLAIPQEIIVSYEPSDKDALETYLREQTGRRVTIL
                     VPQKGDKKKRLATAQENAAYQLKLELLKKSRRHNGLADLAKIIGLGSIPRRIEAFDIS
                     NLGSTHIVGASIVFIDGQPAKGEYRKYKIETPEGQNDFASMRELVFRRMHNKNRPTPD
                     LLLIDGGKGQLSAAGEALQLAKADVPFISLAKKEELIFTPGSSDPIVLNQNSDALLLL
                     TSIRDEVHRFVITFHRQRRSKSLLK"
     gene            complement(6735..7574)
                     /locus_tag="VGE59_05155"
     CDS             complement(6735..7574)
                     /locus_tag="VGE59_05155"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY1075046.1"
                     /translation="MIIGAHVSAAGGIMNAIPRAKEIGASAVQIFASAPGNWNPPKAT
                     LEEGRAFGEACRSEGITHIFFHSIYLINLASDKPFLIHKSKESLIAGLNLNAAMGGNG
                     VIFHVGSTKDRTFDAAKENIIKSLNDIIAKSDPKSILIIETNAGQGNCVGDTFEEVAE
                     LIAGVEQKERIGVCLDTAHTFASGYDLVNVGPESIMQQFDEIIGLKYLKAIHCNDSKT
                     DFNSRVDRHENIGLGKIGEEPFRQLLHLPELKNIPFILEVPGIEGNGPDRINVDKLKD
                     LAV"
     gene            complement(7577..8512)
                     /gene="trxB"
                     /locus_tag="VGE59_05160"
     CDS             complement(7577..8512)
                     /gene="trxB"
                     /locus_tag="VGE59_05160"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY1075047.1"
                     /translation="MSQPLPSHTKVAIIGSGPAGLTAAIYAGRALLNPFVIAGREVGG
                     QLMTTTDVENFPGFPGGVMGPELMTKMREQAERFEVDFLEENVVSIDTQAKPFSIKTE
                     SHSLTADAVILATGASAKWLGLPSEQRLRGHGVSSCATCDGFFFRGKEIAVIGGGDSA
                     MEEATFLTRFASKVYLVHRREEFRASKIMLEKAKSDPKIEFVLNSEIEEVLGEEKVSG
                     LKIKNNQTNEVSTLSVEGVFVAIGHQPNSGFLAGAIEVDATGYVVIKDDYHTSVPGIF
                     VAGDLHDHKYRQAITAAGAGCAAALEAERYLARKD"
     gene            complement(9043..>9243)
                     /locus_tag="VGE59_05165"
     CDS             complement(9043..>9243)
                     /locus_tag="VGE59_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1075048.1"
                     /translation="RFKGEFADVGKALDSARNKYEQVATTRYKQIDRLTTKINSYQLE
                     QDDPPQLIEAPLEVIETLEVSE"
BASE COUNT         2646 a         2192 c         1975 g         2430 t
ORIGIN      
        1 gaacaagttg tcgaacagat cattcgacca actggtttaa ttgatcccaa gctaaccctc
       61 caccagaccg ccgatcaggt agcccacctc ctggagaata tcaagcttcg tgtcgcaaaa
      121 aacgagcgaa cccttgtcac cactcttaca aagaaaatgg ctgaagactt aagtgaatat
      181 tttatagggc aaggccttaa agttcggtac cttcactccg acgttgatac aatcgagcgc
      241 atcgaaattt tacgtgattt acgaaaaggg atatttgacg ttttagtcgg tgtaaacctt
      301 ttgcgggaag gcttagactt acctgaagta tcgctcgttg caatcctcga cgccgataaa
      361 gaaggttttt tacgctccga acgttcactc atccagacga taggacgagc ggcccgtaat
      421 gttgacggcg aggtaatcct ttacgccgac gttatgaccg gttcgcttca acgtgctatt
      481 caagaaacag aacgacgccg cgctatccaa attgcctata acgaaaagca tggcattacc
      541 cctcaaagca ttcagcgtga aattgtttct atcgtcaacc gagaaattga gggagaagag
      601 acacacaaac tcgaccaagt cgaacgcgaa atggcgcgtg gaaacctaga cgcagtcatt
      661 aaacaaaagg agcgtgaaat gaaagcagct gctaaggatc ttcaatttga attagcggca
      721 ttactcagag acgaactggg agagcttcgc aaactccaac gacagcaagt tagcaactta
      781 ctttcatagt ggagagttac cctattcaac attctcagtg gtggtagttg attcgcttaa
      841 tccttgccta tcacaattaa ctcgaatatt gagaaattcc tctttaacaa gcaagggttc
      901 aataaagtct tgaacatctt gcagttggat tgagtctaac aatgcgacat actcagtgag
      961 tggaataatt ctaccgtaat cttggaaatc atcgactgct tcatcattaa gggagccaac
     1021 actatattct cggtgcaaaa ttgattgctt aatgctatgt ttaacttcct ggaatcgttc
     1081 atcattagaa agaatggccc gcatttgctc ccatgcaatg gtatccactt gatccatata
     1141 acgatactgg gtcgaaatcg tgaacatgag aagggttaga tcctgataaa tgcctgcatg
     1201 cattcccatg ctataggcga gccctcgttt ttcacgtatc tcttgataca aggggctcgt
     1261 aaaaccacgg gttagcatgt cagtgtatag ttttaaaatt gtagcaatgc gtaaatcggc
     1321 tttcggcaat acccgtccta atgcatacac agaacggtta tactcgcttg gggtatatgt
     1381 ttgaactggt tcgacaatag aatgaggctt taattcgaga ttccgttctg ttttttcccc
     1441 tttatgagta attgagccaa agaaggtctc acagtgagaa agtaattcat ctgacttgaa
     1501 atctcctgta aaaaagaggg tcagattatg agagttatag tacttttcat gaaatgcaac
     1561 gaggtcttct tgtgcaatac tgttcagcgc ctgttctgta cctatcgttg cgtagaccaa
     1621 cggatgatta ccaagtaacc cttccatgac tttacttcgt acaaagaagt taacatgggc
     1681 atgcttattc cgtaattctt ccaggataac gcttcgttca atttctatat cttcagggcg
     1741 taaaatagga tagagaaaca tgtccgccac gcgcgcaatt gccccttcaa tcatacttgt
     1801 tggagcaatt acttcgtaac cagtgacttc atcagatgta aatgcattca catacccacc
     1861 attattttcg atatcctgag ttaactcagc cagagtagga aaatgcttcg tacctcgaaa
     1921 gggcatgtgc tcgaaaaagt gagccgtccc ctctttgccg cgaggatcgt ctcgcccccc
     1981 cactgtcacg agggcacagc aagcaatttt aggcagccca aagagctgtt ctgcctgttt
     2041 gtaataaatt gatataccat tagaaagcgt tgtgtgagta aatatgcctg ggtctttgga
     2101 taaaggtaaa tgaatcatgt tcgaaccata tcatgagtcg aatgatgaat gaagggcatt
     2161 gctaccaata aaatagtact caagagacaa atctcgtgac ggaagaacta aaaagtgtta
     2221 caatgacaac gcttacaaaa tatatgcaag acgataaaat aattatccgc ggcgctcgag
     2281 agcataatct caaaaatatt gatcttgagt taccgcgcaa tagcctcatc gcatttactg
     2341 gcttatccgg atcaggtaag tcatctttag cgttcgacac gatttatgcc gagggtcaac
     2401 gtcggtatat ggagagcctt tcaagctacg cccgccaatt tttgggtcag atggaaaagc
     2461 ctgacgtcga tagtattgat ggcctctcac ctgctatctc catcgatcaa aagtcgacca
     2521 gccataaccc acgatcaacg gtaggaacga ttacagagat taatgactat ttacgccttc
     2581 tctatgcgcg gattggcgta ccacactgtc cggtctgcaa tcgtattatt tctcgtcaac
     2641 caactgaaca aattgttgat actattttac gttatcctga aagttcacgc gtattgattt
     2701 tagctcctgt tgtgcggggc cgtaaaggcg aatatcacca actcctctat gatatgtatc
     2761 atggtggatt tgctcgtgtt cgagttaacg gcgaaattcg gtctctttcc gattctattc
     2821 agcttgatcg ctacaaagcg cacgatattg atattgtgat tgaccgttta actattgggc
     2881 atgttatgac gagccgcgtt caggaagcag tcgagcaggc cttaaaactc acgaaaggcc
     2941 tcgtaacaat acttaacgaa acgaccggcg aagaagttat tctcagccaa caattcgcct
     3001 gccctgtcga tggaacatca atcgaagaac tgactccccg atcattctcc ttcaacagtc
     3061 cttttggcgc ctgcgaaaca tgtaatgggt taggatatat ccgcgagctt gatgcgaccc
     3121 ttttaatgcc agacacgagt aaatcgatcc aagatggggc aattatgccg tggcactacc
     3181 gaggcaataa ctactatggg tcaattatta aatcaattgc tcaacactat aatatttctc
     3241 tcacgcagcc acttaataaa cttgataaga aaaagttaga caccattatt cagggggcag
     3301 ctgagccagt tttaatccct gcaacataca tgacatcaac ggggcccaaa cggtttagta
     3361 ttcgttttca aggtctgatg aaccatttgc aaaaacgcta ccaagaaacg gaaagcgatg
     3421 ccgtacgatc tgatattgag cgatacatgg cggcaaaaca gtgtgcaacg tgtcaaggac
     3481 aacgtttacg ccataactcc ctgctggtta cgatttatga taaaaatatc gccgaaatag
     3541 gtgagttcac tattgaggaa gctctcaatt ggtttcgagg attaaaactt aacgataagg
     3601 aaatgcaaat tgcgctccaa attgtgttcg aaattaccaa ccggcttgag ttcctatata
     3661 aggtaggact aaactacctg accctgtctc gaagtgcagt gactctttcc ggtggagaag
     3721 cgcagcgcat tcgtttagct agccaaattg gttcggcctt aactggcgtt ttatacgttc
     3781 tcgatgagcc aactattggg cttcaccaac gtgacaatac acgcttgatt gaaacactca
     3841 aaacactcaa ggatcgagga aacacaatta ttgtcgttga acatgatgaa gataccatcc
     3901 gggcttcaga ttatgttgta gacatagggc ccggtgcggg agaacatgga ggcaatgtag
     3961 tagctgcagc tgaacgatca gcatttttag cggacgatag ctctataacc gcccgttatc
     4021 tgagcggcaa agagcttatc ccaactccag aaaaacgacg acaggcaaat aagcttcgag
     4081 aagttaccgt tcaccatgcc cgagaaaata atcttaaaga tatcaccgtc tcctttccac
     4141 ttggtctcat gacgtgcgtt accggcgttt ctggaagcgg caaatcaact ttggttaacg
     4201 aaattcttta taaaggtctg actaatatcg tgaaccggac aaatcatccg gttgggaagc
     4261 acgataaaat ttcgggtgca gaagaagttg aacgcgtcat tattatcgac caatcaccta
     4321 ttggacgaac tcctcgaagt aacccagcta cctatacgaa gtcttttgac catattcgag
     4381 acttattctc gcgagctcca gaagcaaaaa tacgtggtta taaacctgga cgatttagct
     4441 tcaacgtcga cggcggccgt tgtgataact gtaaaggaga tggtgtcatt cggattgaaa
     4501 tgcacttttt ggccgacgta gagatcccct gcgaagtctg taaaggcaca cgctataacc
     4561 gtgaaactct tgaagttcga tttaagggta aaaatatttc acaagtatta aatatgacag
     4621 tagatgaagg tgtggaattt ttctccagta tcccaagtct ccacgacacc ttactcactc
     4681 tccaagcagt cggcttagga tatatccggt taggccagag cgccacaact ctttctggag
     4741 gggaagcaca acgtgtaaaa cttgccgccg aacttgggaa acgaacacat ggcaagagct
     4801 tatacatcct cgacgaacca accaccgggc ttcactttga agacatccga aagcttttag
     4861 aagtattgaa ccgattagtt aacaagggta acaccatcgt cattatcgaa cacaatcttg
     4921 atgttattaa aaatgctgat tggataattg acttggggcc tgagggcggg gtccaaggcg
     4981 gcacaattgt cgccaagggt accccagaag aagtcgctga agtaagcagt tcctacaccg
     5041 ggcattatct tcgtattcat cttgatcagg caaaacaata caagaaaatc gcacttaaaa
     5101 cccgggcaaa agcataggta gcgccatgac tgagcacatt aaagaaaagc tccgtcactt
     5161 acctcatagt cctggcgttt accactattt cgattcgcaa cagaatcttc tgtacgtcgg
     5221 taaagcaaag gaccttttta aacgcgtcca ttcttactgg aataaacagc atcaatcgcc
     5281 ctggacgcag ctccttgtcg aagagattga tgacattaaa ataatcgagg ttaaaaccga
     5341 gctagaagcg ctcatgctgg aaaatagcct tatcaagtca ctgcgcccaa aatataatat
     5401 tcaactgcgt gacgacaaaa cattcccttt tctccgtgta tcccgggaag attttcctac
     5461 ttttggcatc acccggaagg tgaaagaaga cggcgcacgc tactttggcc cttatctatc
     5521 agcttcgtac cttcgttcca tgctcaagtt actgcagagt ctctatggaa taaaaaccgt
     5581 cagcgaaaag tcgtatgaat cacgctcttc cgtcccgaat cagattggtc tcggtgcccg
     5641 caatcttgaa aaccgagaag catataacca aaatgttaaa gaggcgatcc gctttctctc
     5701 ttctccgcag ccagagatgg aacgagagat caaacaagca atggacacgg ccgctcataa
     5761 tgaagaatat gagcgagcgg ccatactccg cgatcgactt ggtgcagtac agcagctacg
     5821 gcagaatcag tcactcttta gcccgaaagg actcagcacc gattatatcg ggtggactca
     5881 agcaggaaaa cttataacgg tatatgtctt gatggagcga gagggcaaaa ttcttgataa
     5941 acgtgatttt gtttttgagt ttccttcccc actcacacca aacgaactta cagacgaaat
     6001 actggattac ctctacatcc agggattagc tatccctcaa gaaattatcg tttcatatga
     6061 gccctcagac aaagatgccc tcgagacata tttgcgagaa caaaccggcc gtcgcgtgac
     6121 cattcttgtg cctcaaaaag gtgataaaaa gaaacgctta gcaacagcgc aagagaatgc
     6181 agcgtatcaa ctaaaacttg aactgctaaa gaagtctcgg cgacacaacg gtttggctga
     6241 tctagccaag ataatcggcc ttgggtctat tccacgccgg atcgaagcat ttgatatctc
     6301 taacctcgga tcgactcata ttgttggtgc ctcgatcgtc tttattgatg gccaaccggc
     6361 aaaaggtgag tatcgaaaat acaaaataga aaccccagaa ggccaaaatg acttcgccag
     6421 tatgcgagag ctcgtcttcc gtcgtatgca caataaaaat cgccctacgc ccgacctact
     6481 gcttattgac ggaggaaaag gacaactttc tgctgccggc gaggcccttc agctggccaa
     6541 agccgacgtt ccttttattt cgttagcaaa aaaagaagag cttattttca cacccggcag
     6601 cagcgatcct attgttttga accaaaattc ggatgcgctc ttactcctga caagcatacg
     6661 agatgaagtg caccgattcg ttataacgtt tcatcggcaa agaagaagca agtcactgct
     6721 aaagtagatc tcgattatac ggccaagtct tttaacttat ctacgtttat acggtcaggg
     6781 ccgttcccct cgatacccgg aacttcaaga atgaacggaa tattttttaa ttccggtagg
     6841 tgaagcaact gacggaatgg ctcttcgcca atctttccta atccaatatt ctcatgccga
     6901 tcaactcgcg agttaaaatc ggtttttgaa tcattacaat ggatagcttt caaatacttt
     6961 agcccaataa tctcatcaaa ttgctgcata atagactccg ggccaacatt caccaggtca
     7021 tatcccgaag caaacgtatg agcagtatcc agacaaacgc cgatgcgttc tttttgctct
     7081 acgccagcaa taagctcagc cacttcttca aatgtatcgc caacacaatt cccttgtcct
     7141 gcatttgttt caataatgag tatcgacttc ggatcgctct tggcaataat atcgttcaag
     7201 gatttaataa tgttttcttt tgccgcatca aaggttcgat ctttggtgct tcccacgtga
     7261 aaaataaccc cattacctcc catagcagca ttcaaattaa gcccagctat aagagattct
     7321 tttgatttgt gaattaaaaa tggcttatct gaagctaagt taataaggta aatgctatga
     7381 aagaaaatat gggtaatacc ttcggagcga cacgcctcac caaatgctct tccttcttca
     7441 agggtcgcct tgggaggatt ccaattacct ggggcagagg caaatatttg aaccgcactc
     7501 gcaccgattt cttttgcgcg agggatggca ttcataatcc ctccagcggc actaacatga
     7561 gcaccaatta tcatgcttaa tccttacggg ccaaatagcg ttctgcttct aacgctgccg
     7621 cacaacctgc gcctgcggca gtaatagcct gccgatattt gtgatcatgg agatctccgg
     7681 ctacaaatat acccggaaca gatgtgtgat aatcatcctt tatcacaaca taccctgttg
     7741 catcaacctc aatggctccg gcaagaaatc cagaattggg ctgatggccg atcgccacaa
     7801 acactccttc tacggatagg gtggagactt cattcgtttg gttattcttt attttaagcc
     7861 ctgagacttt ttcttctccc agcacttctt caatttcact atttaaaaca aattcaatct
     7921 tcgggtcaga cttcgctttt tcgagcatga ttttgctcgc acgaaactct tcgcgtcggt
     7981 gcacgagata caccttactt gcaaagcggg tcaaaaatgt tgcttcttcc atagcactat
     8041 cccccccgcc gatcactgca atttccttgc cccggaagaa aaatccatcg catgtggcac
     8101 agctggacac accgtgaccg cgtaatcgtt gctcacttgg gagaccaagc catttagctg
     8161 atgccccagt agccaaaata actgcatcgg cggtcagtga atggctttct gtcttgatcg
     8221 aaaatggctt tgcttgtgtg tcgatggaaa cgacgttttc ttctagaaaa tccacttcaa
     8281 aacgttctgc ttgctctcgc atttttgtca tgagctcggg tcccatcacc ccaccaggaa
     8341 acccgggaaa gttctcaaca tcggttgtgg tcataagttg tcccccaacc tctctgccag
     8401 caatgacaaa cggattaagt aaagctctac cggcgtaaat agcggcggta agaccagcgg
     8461 gcccggaacc gattattgca actttcgtat gggaagggag aggttgtgac acgttactat
     8521 cctttattat ttaacggcgt ataaaaattt aattctcgct gcgttttatt taaacacact
     8581 caccaacgct tgacaaacta gccaacatat ggtatattcc gatacaatca ctgtgatggc
     8641 actttctaag ccatcgtatg aggtaattac gtgaaaagag ttacgacact cgtgttcggg
     8701 ctgatcacgc ttgccgcaat gctggggctc cttgatggtc cagtggatgc tatggcggca
     8761 gcaatcccgt ctccaacctt tgaaacggtg tatgccgccg tagagggcat cataaccctc
     8821 agtaaaatag ttggagtctc gctcatcgca ttcgtcgggt acgcgacgtg gcggattgca
     8881 atccgccccc aggtacgtcg gtggaacaag cggcggaaag ccgcctctac cagactcgcg
     8941 atttcgcagt atccgcacgc cgcatgaagc attccttccc ctacggtgta gtccttaagc
     9001 cgtagatgtc tctcccctcg tgggaagcgt ctacggcttt tgttattcgc tcacttccag
     9061 ggtctcgata acttctaacg gagcttcaat aagctgagga ggatcatctt gttctagttg
     9121 atagctatta atcttcgtcg ttaaacggtc gatctgttta tagcgggtcg tcgccacttg
     9181 ttcatattta ttgcgagccg agtcgagagc cttgcctaca tccgcaaact cgcctttgaa
     9241 acg
//
